LOCUS       DASUAP010000001         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1185618, whole genome shotgun sequence.
ACCESSION   DASUAP010000001 DASUAP010000000
VERSION     DASUAP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185618"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFU45_00005"
     CDS             <1..363
                     /locus_tag="VFU45_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5357469.1"
                     /translation="VFGEQFWRPYVHVRDVARAVTAVLGAPVADVQNAVFNVGGTAEN
                     YTKKMLVDLILERLPGTNVEFVKRDADPRDYRVRFGKIEQQLGFRPTFVVPRGLDEVI
                     TAIRTGVIADFTRKDFRN"
     gene            438..1577
                     /locus_tag="VFU45_00010"
     CDS             438..1577
                     /locus_tag="VFU45_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HEU5357470.1"
                     /translation="MCEPYVAGNEWRYVKECLDTGWVSSVGSFVDRFEKEFAARVGVR
                     FAVAMSSGTAALHIAALVAGIEPGDEVVAPAVTFIAPLNAMRYAGAWPTVVDVEPDYW
                     QLDAREARRFLADDCERRDGVLVNRHTGRRVRALMPVDILGHPVELDAIHALADEFAL
                     TVIEDATESLGARYQDRPLGQRSPITCFSFNGNKLITTGGGGMLVCDDETTAKRARYL
                     ATQAKDDPLEYVHGIVGYNYRLTNVLAAMGCAQLEQLDAFIAKKRGIAARYTEALRDV
                     PGITPMREAPWAFSAFWLFTVLVDEGRFGMDSRALLRRLADAKIQCRPLWQPMHQSAA
                     HAGSWSRACPVAESIHARALSLPCSVGLTDGDQERVIAAIRAAAR"
     gene            1574..2218
                     /locus_tag="VFU45_00015"
     CDS             1574..2218
                     /locus_tag="VFU45_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU5357471.1"
                     /translation="MTPAPDRPIVVLGGGGHAKVVIAVLKRSDRRILGYTDQQDRGEI
                     LSGPYLGTDAALAGVLRSHRGCSAVVAVGKIDASPRRLALQAEAGALGFEFPAIVSPH
                     AIVNEAVQLGPGTVVLDGVVVNSGTETGGACILNTGSTVDHDCRLGDNVHIAPGVTLS
                     GGVTIGDHCLIGAGTTVIQGVTICAGTLVGAGATVVKDLTAPGTYAGVPARRLH"
     gene            2215..2922
                     /locus_tag="VFU45_00020"
     CDS             2215..2922
                     /locus_tag="VFU45_00020"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEU5357472.1"
                     /translation="MKSIAVIPARGGSKRVPGKNVKPMFGKPLIAYTIEAAQQSGLFQ
                     RIVVTTDSEEIAATARRFGAEVPFLRGQNLADDVTPVSAATVDVLLRLDPAGDVFDYA
                     CQLMANCPLRTAEDILDSYRQFATTGADSQLSVVKYGWQNPWWALRRDANFRLEPVFK
                     DYIMQRSQDLPEIFCPTGAIWWAKTPVLRREGTYHIANRTGWAMSSWERALDIDTEED
                     WALVEALMGLARARSGA"
     gene            2919..3839
                     /locus_tag="VFU45_00025"
     CDS             2919..3839
                     /locus_tag="VFU45_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357473.1"
                     /translation="MTTTLALYTTVYPGVEPYLREWYRSVQEQTDRDFQLWIGLDTLS
                     VDAVREALGADPNATYVAARPADTPSEVRQRALARVVDAHDAVVLVDSDDILYPSRVA
                     TARAALRESDVASCAIRVVNGQGKDLGLTFALPPSGGVDAVLPRYNIFGLSNSSFRSS
                     VLKRCLPIPAGAVMVDWYLATLAWLLGARFRADTEVGMAYRQYGSNTARVLPPFDADQ
                     VTRDTDRVRQHFGRVQSAALPGASLDRRDDIGAAAAEVEAFYRQVVLHPAVFDNYLRH
                     LNTLTPASYWWSCVAHPALRELWAPARSLS"
     gene            3836..>4428
                     /locus_tag="VFU45_00030"
     CDS             3836..>4428
                     /locus_tag="VFU45_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEU5357474.1"
                     /translation="MNVVKLGDIPIGADQPPYVIAEVGSNHNGDMDLCRRLIDAAAAA
                     GAHAVKFQSWSESSLIAKEEYDRNPSYSDKKKHFGSLRDMVKAYQFTPQMHREADAYC
                     RTRGIAFCSSVFAPDEADLLESLNVPFYKIASMDAVNLPLLDYVARKGRPVVISTGMC
                     TLAEIERAVETVRRAGNEQTVLLHCVSIYPPDYEMIHL"
BASE COUNT          688 a         1501 c         1520 g          719 t
ORIGIN      
        1 gtgttcggcg agcagttctg gcgtccctac gttcacgtcc gggacgtcgc tcgcgcggtg
       61 accgcggtgc tgggtgcgcc ggtggcagac gtgcagaacg cggtattcaa cgtcggcggc
      121 accgccgaga actacacgaa gaagatgctg gtggacctga ttctggagcg gctgcccggg
      181 acgaacgtgg agttcgtcaa gcgcgacgcg gatccccgcg actaccgggt ccgattcggg
      241 aagatcgagc agcagctcgg cttccggccc acgttcgtcg tgccccgcgg attggacgag
      301 gtgatcaccg ccatccgcac cggcgtcatc gcggacttca cccgcaagga cttccgcaac
      361 tgatcccccc ctccggcggt acggccgaag cgcgccgatt cgagccgggc gccccggcta
      421 cgggcgggtt catcggtctg tgcgagccgt acgtggcggg caacgagtgg cgctacgtga
      481 aggaatgcct cgacaccggc tgggtctcct ccgtcggcag cttcgtggac cggttcgaaa
      541 aggagttcgc ggcccgggtc ggcgttcgct tcgccgtggc gatgagctcc ggtactgccg
      601 cgcttcacat cgccgccctg gtggccggga tcgagccggg cgacgaggtc gtcgcacccg
      661 cggtgacctt catcgctccg ctcaacgcca tgcgctacgc cggtgcgtgg cccacggtgg
      721 tcgacgtcga gcccgattac tggcagctcg atgcgcgcga ggcgcgtcgc tttctcgcgg
      781 acgactgcga acgacgggac ggcgtcctgg tgaaccgcca cacggggcgc cgggtgcgtg
      841 ccctgatgcc cgtggacatc ctcggccatc cggtcgagct ggacgcgatc cacgcgctgg
      901 ccgacgagtt cgccctgacc gtcatcgaag acgcgaccga gtccctgggc gcgcggtacc
      961 aggaccgccc cctcggccag cggagcccga tcacgtgctt cagcttcaac ggcaacaagc
     1021 tgatcaccac cgggggcggc gggatgctcg tctgcgacga cgagacgacc gccaagcggg
     1081 ctcggtacct cgcgacccag gccaaggacg atcccctgga atacgtgcac gggatcgtcg
     1141 gctacaacta ccggctgacc aacgtcctcg cggcgatggg ctgcgcgcag ctcgagcagc
     1201 tcgacgcgtt catcgcgaag aagcgcggca tcgccgcgag gtatacggag gcccttcggg
     1261 acgtgcccgg catcacgccg atgcgggagg cgccttgggc gttctccgcg ttctggctct
     1321 tcaccgttct ggtggacgag gggcggttcg ggatggacag ccgggcgctg ctgcggcggc
     1381 tggccgacgc gaagatccag tgccggccgc tctggcagcc gatgcatcag agcgcggcgc
     1441 acgccggatc ctggagccgg gcctgcccgg tggcggagtc catccacgcc cgtgccctga
     1501 gcctgccgtg ctccgtcggc ctcaccgacg gggatcagga gcgcgtcatc gcggcgatcc
     1561 gcgccgccgc gcggtgacgc ccgcgccgga ccggccgatc gtcgtgctgg gcgggggcgg
     1621 tcacgccaag gtcgtgatcg ccgtgctgaa gcggtccgac cggcgcatcc tcgggtacac
     1681 ggatcagcag gaccggggtg agatcctgtc ggggccctac ctcggcaccg acgccgcgct
     1741 cgcgggtgtc ctccgctcgc atcgggggtg ctcggcggtc gtcgcggtcg gcaagattga
     1801 cgcatcgccc cgccggctgg ccctgcaggc cgaagccgga gcgctcggat tcgaatttcc
     1861 cgccatcgtt tccccccatg cgatcgtcaa cgaagcggtg caactgggtc ccgggaccgt
     1921 ggtcctggac ggcgtggtgg tgaacagcgg caccgagacc ggcggcgcgt gcatcctcaa
     1981 caccggcagc accgttgacc atgattgccg cctcggagac aacgtccaca tcgcgcccgg
     2041 cgtcacgctg agtggcggtg tcacgatcgg cgaccactgc ctgatcgggg cggggacgac
     2101 cgtcatccag ggcgtcacca tctgcgccgg caccttggtc ggcgccggcg cgaccgtggt
     2161 gaaagacctg acggcgccgg ggacgtatgc cggcgttccg gcccggaggc tccattgaag
     2221 tcgatcgccg tgatccccgc ccggggcggc tccaagcgcg tccccggcaa gaacgtgaag
     2281 ccgatgttcg gcaagcccct gatcgcctat acgatcgagg cggcccaaca gagcgggctg
     2341 ttccagcgca tcgtcgtcac cacggacagc gaggagatcg ccgccacggc gcggcgcttc
     2401 ggcgccgagg tccccttcct ccgcggccag aatctggccg acgacgtcac gccggtctcg
     2461 gcggcgacgg tggatgttct cttgcgtctc gacccggccg gggatgtctt cgactatgcc
     2521 tgccagctga tggccaactg cccgctccgg actgccgaag acatcctgga cagctaccgc
     2581 cagttcgcga ccaccggtgc ggattcccag ctgtcggtgg tcaagtacgg atggcagaac
     2641 ccgtggtggg ccctgcggcg cgacgccaac tttcggctgg agcccgtgtt caaggactat
     2701 atcatgcagc gcagccagga cctgcctgag atcttctgtc ccaccggcgc catctggtgg
     2761 gccaagacac cggtcctccg gcgggaaggc acctatcaca tcgcgaaccg gaccggctgg
     2821 gccatgtcgt cgtgggagcg ggccctcgac atcgacaccg aagaggactg ggcgctggtc
     2881 gaggcgttga tggggttggc ccgggctcgc tcgggggcat gacgaccacc ctcgccctgt
     2941 acaccaccgt gtacccgggc gtcgagccct acctccggga gtggtatcgc tccgtgcagg
     3001 agcagaccga ccgggatttc cagctctgga tcggcctcga cacgctctcc gtcgatgcgg
     3061 tgcgcgaggc gctgggagcc gatccaaacg cgacctacgt ggcggcccgc ccggccgaca
     3121 caccgtcgga ggttcggcaa cgggccctcg cgcgagtcgt ggacgcacac gacgcggtgg
     3181 tgctggtgga ctcggatgac atcctctatc cctcacgagt cgccacagcg cgagccgccc
     3241 tgcgggaatc ggacgtcgca tcctgcgcaa tccgcgtggt caacgggcag ggcaaggacc
     3301 ttgggctgac gttcgccctt ccgccctccg gtggtgtgga cgcggtgttg ccgagataca
     3361 acatcttcgg tctttccaac tccagcttcc ggtcctcagt gctcaagcgc tgcctgccga
     3421 tcccggcggg cgcggtgatg gtggattggt atctggcaac gctggcctgg cttctcggcg
     3481 ccaggttccg agcggacacc gaggtcggca tggcgtaccg acagtatggt tccaatacgg
     3541 cacgggtttt gcctccgttc gacgccgacc aggtcacccg ggacacggac cgcgtccgcc
     3601 agcactttgg ccgggtccag tcggccgctc tgcccggcgc ttcgctcgat cgccgcgacg
     3661 acattggtgc ggcggcggcc gaggtggagg cgttctaccg gcaggtcgtg ctgcacccgg
     3721 cggtgttcga caactacctc aggcacctga acacgttgac gcccgcatcc tactggtggt
     3781 cttgtgtggc gcacccggcg ctccgcgagc tgtgggcacc ggcaaggagt ctgtcatgaa
     3841 cgtggtgaag ctcggggaca ttcccatcgg cgccgatcaa ccgccgtatg tgatcgcgga
     3901 ggtcggatcc aatcacaacg gcgacatgga tctgtgccgg cgcctgatcg acgcggcggc
     3961 ggcggcgggg gcgcacgcgg tcaagttcca gtcctggagc gaatcctcac tcatcgccaa
     4021 ggaggaatac gaccggaacc cgtcgtattc cgacaagaag aagcacttcg gctcgctccg
     4081 cgacatggtg aaggcctatc agttcacgcc ccagatgcat cgggaggccg acgcctattg
     4141 ccgcacgcgg ggaatcgcgt tttgctcgag cgtgttcgct ccggacgaag cggacctgct
     4201 cgagagcctg aacgtgccct tctacaagat cgcgtcgatg gacgcggtca acctgccgct
     4261 gctggactac gtggcgcgga agggacgccc cgtggtcatc tccacgggga tgtgcaccct
     4321 ggcggagatc gagcgggcgg tcgagacggt gcgccgtgcg gggaacgagc agaccgtgct
     4381 cctgcactgc gtgagcatct atccgcccga ctacgaaatg atccatct
//
LOCUS       DASUAP010000002         4933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1117511, whole genome shotgun sequence.
ACCESSION   DASUAP010000002 DASUAP010000000
VERSION     DASUAP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117511"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VFU45_00035"
     CDS             complement(<1..1052)
                     /locus_tag="VFU45_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5357475.1"
                     /translation="MGSSTSAERTFRDGWIAAPLIDAGVLTRAQVERARADGVPELWR
                     AAVAEGWAASDRIIGVLAERFGFPVADLERAEHRVMALVPEAVARRHRVLPIAANGRR
                     IIIATADPRDLSVERDLAFLTGRTVEFQLAAPEALLARLDELYRPERSIERLVAGLGQ
                     RATQTAVETGPAAGVLKDPVLEAPVARLVGALIAEGVRERASDIHFDPEEGGMAVRFR
                     VDGVLREVMRLPDAAGGSLVRRVKILADLDVTNPLVPADGRASVRVDGQPVDLRVATA
                     PVARRGQKAVIRILDPANLKTRLSDLGLVPSEHAGLTRLLGHREGIVLVTGPTGSGKT
                     TTLYAALNQLKTGKVN"
     gene            1127..1972
                     /locus_tag="VFU45_00040"
     CDS             1127..1972
                     /locus_tag="VFU45_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357476.1"
                     /translation="MRPDSLAVLGLGATGASAAWSARTAGVPRVLGWAASRADATSAL
                     RAGAVDDLTDRADRAVTGAAVVLVADAPGTADQVLRRVAGSLRPDALVLLLSEVHGAA
                     LATAGALGLGDRTAALDPMGESPGPGFAGAGPDRLRGRLTYVSSTDTEAGHQAARSAM
                     SFVEEILGGAPVLIDPARHDEQVAWTRQLPGAALAVLCHLLDGRRLGGVTWDAAARSA
                     RAGVPADADVAADVLLADRRAVAMALGALGAEVEVLRKLVDEGNGAGVRAFLERAAQF
                     RPGPS"
     gene            complement(1965..2426)
                     /locus_tag="VFU45_00045"
     CDS             complement(1965..2426)
                     /locus_tag="VFU45_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357477.1"
                     /translation="MIRSRRGIALVELAVALAALLVLAGLIAPMIRARRVRVRAIRIV
                     AEVKKGVVALGNYDREHNQWPASAGTGLTPAGLGSGPTGPTFRADDYVLRYTLRPSTT
                     PDGPVEHPIIVVRPTDPRACSDVYDGLGGEENPNAFAYCSTESGAVYLFLN"
     gene            complement(2423..2944)
                     /locus_tag="VFU45_00050"
     CDS             complement(2423..2944)
                     /locus_tag="VFU45_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357478.1"
                     /translation="MPAEVQAVDPGNRDAIYASIAELEEQAWRRREAEEALPAPPPPP
                     TRRWSVAIALGALAVVIWVMPLVTRPRIHVVTVVPRNAPAARVERALQTVADLVEQYR
                     LAERRLPASISLAGPVTPQIHYFPDTGGRFILEMPTTRGIARLTVERGGTAFQMFGPD
                     VLRPAPPVLRSRR"
     gene            3089..4366
                     /gene="aroA"
                     /locus_tag="VFU45_00055"
     CDS             3089..4366
                     /gene="aroA"
                     /locus_tag="VFU45_00055"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU5357479.1"
                     /translation="MRVSGNVRVPGDKSVTHRALMLASLAGGTSWIGGALTAFDARSS
                     ARVLRALGAEISPLVPDRTVQVRAGRRFHRPEARLDCGNSGTTTRLLLGLLAAHPFRA
                     ELTGDASLRRRPMRRVTGPLARMGAHFEELGAGDGLPLAVRGGRLHELTWELPVSSAQ
                     VKSALLLAGMAGGVPVRLREPGGRSRDHTERMLRAFGYSVVEAADGWIRFEPGARLAP
                     FELQVPGDPSSAAFLVAAALLAEGGELGVTGVGLNPTRTGFLQVLARMGGDVRQEEVR
                     EEFSEPVGTLVARASALTATTVGGAEIPGLIDEVPVLAVLASRASGTTVFHDVGELRV
                     KESDRLGLLAEDLRRVGVAAEISGDSLHVTGSDRPPRGRVRTAGDHRIAMAFAVLNTV
                     PGADIRIDDLACAGVSFPGFSATLRSLARRPAR"
     gene            4434..>4933
                     /gene="cmk"
                     /locus_tag="VFU45_00060"
     CDS             4434..>4933
                     /gene="cmk"
                     /locus_tag="VFU45_00060"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU5357480.1"
                     /translation="MTDLRVIAIDGPSASGKSSTAQAVARALGLPHLDSGALYRGLAL
                     VALELGIAGKVPAAEVPAEAILAEAERRKLELHPAAGVIGVWLDGVDAEARIRTPEVT
                     GAVSPVSARPAIREWVNARLRRAAAEAGGVVVDGRDIGTVVFPDARLKVFLTATPEAR
                     ARRRSA"
BASE COUNT          616 a         1808 c         1840 g          669 t
ORIGIN      
        1 atgttgacct tcccggtctt gagctggttg agcgcggcgt agagggtggt ggtcttcccg
       61 ctcccggtgg ggccggtcac caggacgatc ccctcccggt ggccgaggag gcgggtgagc
      121 ccggcgtgct ccgacggcac cagccccagg tcggagagcc gggtcttgag gttggcgggg
      181 tcgagtatcc ggatcaccgc cttctggccc cgccgcgcca ccggcgcggt cgccacgcgg
      241 aggtcgaccg gctggccgtc gacgcgcacc gaggctcggc catcggcggg gaccagcggg
      301 ttggtgacat cgagatcggc caggatcttg acccgccgca ccagcgagcc gccggcggca
      361 tcggggagcc gcatcacttc ccggagcaca ccatccacgc ggaaccgcac cgccatcccg
      421 ccctcttcgg ggtcgaaatg gatgtcgctg gcccgctccc gcaccccctc ggcgatcagt
      481 gcgccgacga gccgggccac cggtgcctcg agcaccgggt cctttagcac gccggcagcg
      541 ggacccgtct ccaccgctgt ttgagtggca cgctggccga ggcctgccac gagccgctcg
      601 atgctccgct ccgggcggta cagttcatcc agccgggcga gcagcgcttc cggcgccgcg
      661 agctggaatt ccacggtcct cccggtcagg aaggccaggt cgcgctcgac cgacaggtct
      721 cgcgggtcgg cggtcgcgat gatgatgcga cggccattgg ccgcgatggg caggacgcgg
      781 tgccggcggg cgacggcttc ggggaccagt gccatgaccc ggtgctccgc ccgctccagg
      841 tcggcgaccg ggaatccgaa ccgctcggcg agcactccga tgatccggtc cgacgcggcc
      901 cacccttccg ccaccgccgc ccgccagagt tccggcacgc cgtcggcacg ggcccgctcg
      961 acctgtgcgc gggtgaggac ccccgcgtcg atcagcggcg ccgcgatcca cccgtcgcga
     1021 aaggtgcgct ccgccgacgt tgaggaaccc atggccgata actaccattg gcccatgcca
     1081 tcagcgtcac cgtgcgtgcg ccggacggcg caaaaagacg gcgcgcgtgc gccctgactc
     1141 cctcgcggtg ctgggactgg gcgccaccgg tgcatccgcg gcctggtccg cgcggaccgc
     1201 cggggttccc cgggtgctcg gctgggccgc cagccgcgcc gacgcgacct cggccctccg
     1261 cgccggcgcc gtcgacgacc tgaccgaccg cgccgaccgc gccgtcaccg gcgccgcggt
     1321 ggtgcttgtg gccgacgcgc ccgggacggc cgaccaggta ctgcgccgcg tagcgggaag
     1381 cctccgaccg gatgcgctcg tcctgctcct gtccgaagtc cacggcgccg cgctcgcaac
     1441 ggccggggcg ctcggtctcg gcgaccgcac tgcggcactc gacccgatgg gcgaatcgcc
     1501 cggccccgga ttcgccggag cgggccccga ccggctgcgt gggcgcctga cctacgtgtc
     1561 ctcgaccgac accgaggcgg gacaccaggc ggcccgcagc gccatgagct tcgtcgagga
     1621 gatcctgggc ggggcgccgg tcctcatcga cccggcccgt cacgatgagc aggtcgcgtg
     1681 gacccgccag ctcccggggg cggctctcgc ggttctctgc catctcctcg acggcaggcg
     1741 cctgggcggc gtcacgtggg atgctgcggc gcgctccgcc cgtgccggtg tgccggccga
     1801 cgccgacgtg gcggcggacg tgctcctcgc cgaccggcgc gcggtcgcga tggcgctggg
     1861 ggcgctcggg gccgaagtgg aggtgctgcg gaagctggtg gacgagggca acggggccgg
     1921 cgtgcgcgcg ttcctcgagc gcgccgcgca gttccgcccg gggcctagtt gaggaagagg
     1981 tacacggcac cgctctcggt gctgcagtag gcgaaggcgt tgggattctc ctcgccgccc
     2041 agcccgtcgt acacgtcgga gcacgcccga gggtcggtcg gccggacgac gatgatcgga
     2101 tgctccaccg gcccgtcggg tgtggtcgaa gggcgcagcg tgtagcggag cacgtagtcg
     2161 tcggcgcgga acgtgggacc cgtcggaccc gatccaagcc ccgccggggt gaggccggtg
     2221 cccgcgctgg ccggccactg gttgtgctcc cggtcgtagt tgccgagtgc cacgacgccc
     2281 ttcttgacct cggccacgat gcgtatggcc cgcacccgca cccgccgggc ccggatcatg
     2341 ggcgcgatga gcccggcgag caccaggagc gcggcgagcg ccaccgccaa ctcgaccagg
     2401 gcgatcccgc ggcgcgagcg gatcatcgcc gggagcggag caccgggggc gccggccgca
     2461 ggacatcggg gccgaacatc tggaacgccg tgcccccgcg ctccaccgtg agccgggcga
     2521 tcccgcgggt ggtcggcatc tcgaggatga accggccgcc ggtgtcgggg aagtaatgga
     2581 tctggggcgt caccggcccc gccagtgaga tggaggccgg caggcgacgc tcggccaggc
     2641 gatactgttc gaccaggtcg gcgacggtct ggagtgcccg ctccacccgg gccgcgggcg
     2701 cattccgcgg gacgacggtc accacgtgga tgcggggacg ggtcacgagc ggcatgaccc
     2761 agatcaccac cgccagcgcg ccgagcgcga tcgcgaccga ccagcgccgg gtgggcggcg
     2821 gcgggggagc gggcagcgcc tcttcggcct cgcgccgccg ccacgcctgc tcctcgagct
     2881 cggcgatgga cgcgtagatc gcgtcccggt tgccgggatc cacggcctgg acctcggcgg
     2941 gcaccgccgg cgtactcggg cgcggtggga ccggggcggg tggctcggct atcggatcat
     3001 cgtcagggag cacggccggt cttcaggaga agggtgcggg ggtgaacacc gtcaaactta
     3061 ggcccaacct acagccagcg acagaccgat gcgcgtgagc gggaacgtcc gggtccccgg
     3121 cgacaagagt gtcacccatc gcgcgctgat gctcgcctcc ctggccggtg gcaccagctg
     3181 gatcggcggg gccctcaccg cgttcgatgc ccgctcgtcc gcgcgcgtgc ttcgcgcgct
     3241 gggtgcggag atctcgccgc tcgtccccga ccgcaccgta caggtgcgcg cgggccgccg
     3301 gttccatcga cccgaggcgc ggctcgactg cggcaactcc ggcaccacga cccgccttct
     3361 cctcgggctc ctggcggccc atccgttccg ggccgagctg accggcgacg cgtcgctccg
     3421 ccgccggccg atgcggcggg tcaccggacc gctggcgcgg atgggggcgc acttcgagga
     3481 gctcggtgcg ggcgacgggc tcccgctcgc ggtccgcggc ggccggttgc acgagctgac
     3541 ctgggaactc cccgtctcga gcgcccaggt caagagcgcg ctcctcctgg ccgggatggc
     3601 gggtggcgtg ccggtccgcc tgcgggaacc cggcgggcgc tcccgcgacc acactgaacg
     3661 gatgctccgc gccttcggct actcggtggt ggaagctgcg gacggctgga tccgcttcga
     3721 acccggcgcc cggctcgccc ccttcgagct ccaggtcccg ggcgacccgt cctcggcggc
     3781 cttcctggtg gccgcggcgc tcctcgccga aggtggcgag ctcggcgtca ccggggtcgg
     3841 cctcaatccg acgcggacgg ggttcctcca ggtattggcg cggatgggtg gtgacgtgcg
     3901 ccaggaggag gtccgcgagg aattcagcga gccggtgggg acgctcgttg cgcgggcatc
     3961 ggcgctcacc gcgaccacgg tgggcggtgc cgagatcccg gggctcatcg acgaagtgcc
     4021 cgtgctggcg gtcctcgcct cgcgcgcctc ggggaccacg gtgttccacg atgtcgggga
     4081 gctccgggtc aaggagagcg accggctcgg gctcctggcc gaggaccttc ggcgggtcgg
     4141 cgtcgcggcg gagatctcgg gcgactcgct ccacgtcacc ggcagtgacc ggccccctcg
     4201 cggccgggtc cgcaccgccg gcgatcaccg gatcgcgatg gcctttgccg tgctcaacac
     4261 cgtgcccggt gccgacatcc ggatcgacga cctcgcctgc gccggggtga gctttccggg
     4321 gttctcggct acgctccgct ccttggcccg gcgccccgct cgataaagcg catagattca
     4381 ccccatgttc cgcgacccgc gacccttgac ccgcgacctg tgacccgaac cccgtgaccg
     4441 acctccgcgt catcgctatc gatgggccgt cggcgtcggg caagtcgagc actgcccagg
     4501 cggtcgcccg cgcgctcggc ctgccgcacc tcgacagcgg tgcgctgtac cgcgggctgg
     4561 cgctggtggc gctcgagctt ggcatcgccg ggaaggtgcc ggcggccgag gtgccggcgg
     4621 aggccatcct ggcggaggcc gagcggcgga agctcgagct tcaccccgcc gccggcgtca
     4681 tcggggtctg gctcgacggc gtggacgccg aggcgcggat ccggacgccg gaggtgaccg
     4741 gcgccgtctc gccggtgtcg gcacggccgg ccatccgcga gtgggtcaac gcccggctgc
     4801 ggcgggcggc ggcggaagcg gggggcgtgg tggtggacgg acgggacatc ggcaccgtcg
     4861 tcttcccgga cgcccggctc aaggtgttcc tgaccgctac tccggaggcc cgcgcccggc
     4921 gccggtcggc cca
//
LOCUS       DASUAP010000003        16334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_804079, whole genome shotgun sequence.
ACCESSION   DASUAP010000003 DASUAP010000000
VERSION     DASUAP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 16334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16334
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804079"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..819)
                     /gene="thiD"
                     /locus_tag="VFU45_00065"
     CDS             complement(13..819)
                     /gene="thiD"
                     /locus_tag="VFU45_00065"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU5357481.1"
                     /translation="MPPLLPIALTVAGSDSGGGAGIQADLKTFHQFGAYGTSVLTAVT
                     AQNTVEVRRVDPLDPGLVRAQLDALADDLPPAALKTGMLATGAIAIVVAETIAGRAWR
                     HYVLDPVMTSTSGHRLLDAAAASTVRDRLLPLAELVTPNLDEAEALTGIVVRDPAAMD
                     DAGRRLLALGAGAVLVKGGHLAGDDLVDVLVTRTGIRRFRHRRVATNSTHGTGCTLSA
                     AITAGLALGWELERAVTTALDFVARAIAAAPGLGSGKGPVGHVVQVNGEQ"
     gene            921..1691
                     /locus_tag="VFU45_00070"
     CDS             921..1691
                     /locus_tag="VFU45_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5357482.1"
                     /translation="MSTLLSTIRDLHRRKARERRALALAEGIRLVEEALAAGVAIKGA
                     AIAPALEGTARGQALKAALTARSVPLEVVTDAELVGLADTEHPQGVVAVASPRVWSLA
                     DIHVTPGHPVLVLDGVQDPGNVGTIARTALALGAAGLVALPGTADLLNPKALRGSMGA
                     LFRLPAVHAADAECLDWLTAGGVALWVTDANGEDIRRIGPPTAPVALVLGNEGAGIRA
                     DLLAAGMRRVSIPIAQEAESLNVAVAAGILLHEVTRGR"
     gene            1681..2454
                     /locus_tag="VFU45_00075"
     CDS             1681..2454
                     /locus_tag="VFU45_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU5357483.1"
                     /translation="MAGDPTLTLFGALLGAVLGSFLNVCILRLPADESVVRPPSRCPK
                     CGYALRWFDNIPVLSWVLLRGTCRRCAAPISIQYPLVELATALIWAGFAWAAGPSLEA
                     VRGAVFFTILLGIAMTDAREYVIPDEFSLGGLILGVALHLPDGFGGIGRSLLGAAVGF
                     GVLWLVAVVGKKVFKEDAMGGGDIKMMAMVGAFTGWTGVLLTIFLGSVLGVLIFVPPA
                     ILGKKKLVPFGVFLALGAATTYLVGGAIIDWYRQVAFGG"
     gene            2451..3692
                     /locus_tag="VFU45_00080"
     CDS             2451..3692
                     /locus_tag="VFU45_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357484.1"
                     /translation="MRQRVLVAAVVALAACRGRPPATSDAAELTRLMDSLAPYVEHAT
                     GLRFTRLPRAEILSRDQLRAYLDARFREEYPGQRMRDLRDAYRLLTLLPDSTDLGKLY
                     LDVLSEQVAGFYDPDSAAFFGVAGAPPAFRVSTVAHELVHALQHDYIPLDSILGQKAD
                     ADRLLAAQAILEGQATIAMMRMQPGVGDRVLDPALWETVRDQLAQNQRTMPVFAGAPR
                     VVREGLIFPYLSGAEFMRWWMTTHPDSVQPYGARMPTSTEQILLPSHYLAGDRPVPVS
                     FTAGPPDSADTDVLGDEGVRILLAEIRGQAEVPTAIPIGWGGDRYVIYETAPGPALVW
                     AAVWDTPVAREGFLAAAKGPWLRRVRPGYRMSLEAKDIDGRPGTLFVHAPTGWPGWRD
                     LPVAEARSAPRSPSQAPSAKP"
     gene            3776..5107
                     /gene="ffh"
                     /locus_tag="VFU45_00085"
     CDS             3776..5107
                     /gene="ffh"
                     /locus_tag="VFU45_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU5357485.1"
                     /translation="MFDELSGKLTATLQKLTGRGVLTEEAVREGLREVRRVLLEADVS
                     FELTREFLERVQEKAVGVVALKSVRPGQQIVKIVHDELVALLGERQAPLAFASVPPTV
                     VLIVGLQGSGKTTTAGKLARRLKLEQKAPFLVAADVYRPAAVEQLETLGRQVQVGVHA
                     EPGVTDVVGIVRRGIEAAGKVRARTVLVDTAGRLQVDDELMDELRRLKDAVKPHEILL
                     VADGMTGQDAVRIARGFHEALGVTGVVLTKMDGDARGGAALSIYGVTRAPIKYVGTGE
                     GLDALEPFHPERMAGRILQQGDVLTLVEKAQGVVDQAEAERLAKKAVSKKGMDLQDFL
                     GAMKQMQALGPLKGVLGMLPGVNAQALSKANLDEKKLKHVEAIVLSMTPKERTDPDVI
                     NGSRRLRIAKGAGRPVQEVNQLLAQFGQMKKFMKSAGKPGARMPFGPRSPA"
     gene            5131..5391
                     /gene="rpsP"
                     /locus_tag="VFU45_00090"
     CDS             5131..5391
                     /gene="rpsP"
                     /locus_tag="VFU45_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU5357486.1"
                     /translation="MATRIRLRRVGRKGVPLYRIVVAEKEKARDGRFIATLGEYNPKA
                     KTAAEKVKVNVEQAKAWLAKGATPSDTVAALLRAAGVAVASA"
     gene            5393..5917
                     /gene="rimM"
                     /locus_tag="VFU45_00095"
     CDS             5393..5917
                     /gene="rimM"
                     /locus_tag="VFU45_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU5357487.1"
                     /translation="MAGGSRHLVVGRLRKPHGLKGEYTVFPLTDRPEEVFVAGRRLER
                     WDLGGAVLGEVQVERAKGYHREWLLKFRDVEDRVALDQWRGQFLAAAVEELAPPAEGE
                     VYLHELAGFSVRGPDGVPVGLVSGVYELPQGLMIEVQGPKREFLLPFRKEFVTAVDRA
                     ERRLSVVVPDGLVE"
     gene            5917..6603
                     /gene="trmD"
                     /locus_tag="VFU45_00100"
     CDS             5917..6603
                     /gene="trmD"
                     /locus_tag="VFU45_00100"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU5357488.1"
                     /translation="MLQVNVVTLFPEAFAPWLSASIPGRAAAAGKVGYRLVQLRDFTH
                     DRHHTVDDAPYGGGAGMVLKPEPFFEAVESFRPSGPIVLLSARGRRFGHNDAVRYSLG
                     SELTLLCGHYKDVDERVAAHLATEEVSVGDFVLTGGEPAALCVIDAVVRLLPGALGDH
                     ESASSDSHYDGLLSPPSYTRPPEYRGHRVPEVLLSGDHAAIAAWRAREAERLTQERRP
                     DLWAAYDEDT"
     gene            6657..7004
                     /gene="rplS"
                     /locus_tag="VFU45_00105"
     CDS             6657..7004
                     /gene="rplS"
                     /locus_tag="VFU45_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010334229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU5357489.1"
                     /translation="MEKLATVAREGLRADHPAFDAGDTVKVMVRVREGDKERLQAFEG
                     LCIAKRGGGISESFTVRKVSAGVGVERIFPLHAPTIASIELVRKGRVRRAKLYYLRAV
                     SGKAARIREKREG"
     gene            7007..7636
                     /locus_tag="VFU45_00110"
     CDS             7007..7636
                     /locus_tag="VFU45_00110"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU5357490.1"
                     /translation="MPALRPTLERERAAWAAGKLLVGVDEVGRGPLAGPVVAAAVVFS
                     AGAIPPDGVRDSKTLSASRRDALTPAIRAAALAVAIGAASTAEIDRWNIRRATALAMR
                     RAVARLQRALGAVDCLLLLDGLPLPEIGYPHQALVDGDAHCMSIAAAGIVAKTLRDRL
                     MARLAARHPGYGWETNAGYGTAEHLAALRERGPTRHHRVSFAPVAQLGL"
     gene            7715..10045
                     /locus_tag="VFU45_00115"
     CDS             7715..10045
                     /locus_tag="VFU45_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5357491.1"
                     /translation="MRRLLAVLVLAFLPVTLAAQSNAERILNDHYTRSHDYDLVHQDI
                     EVRDFDWDSLAFKGRVATTLVALTPGMDSVVLDAGALLHVSQVRGAAGPLDFGRRGDT
                     LVVHLSHSADFGDTVRFTVTYDARISNGRGLTFLTGQGRPHRPDQIWSQGEDMNNHFW
                     FPTYDFPNDKATWTLTAVVPARDMAVSNGRLVSNVAARGVRTVRWSEDRPASTYLVSL
                     VVAPLVRIHDQWHGIPVDYYVYPEDSALARPLFRVTPDMIDTYSRLTGVAYPWQKYAQ
                     TTVADFFGGMENVSATTLVDWLPDARAYADRPWYQYILIPHELAHQWFGDLVTTENWA
                     NTWLNEGFAEFMPGQYWGTRLGAHAEQDYYADEYRQFMAIDHRKRMPLAAQGSNNIYP
                     KGALVLEMLKGYLGPERFWASLHRYLTRNAYDNAVTDDLRQAVLDATGENLDWFFDEW
                     VYQAGYPEFTVTQSYDSAAHRVTLVVLQTQVDSSKPDSATGIRYTTPAVFRMPVTVRI
                     GTAAGDVTARDWIDARQDTIVVSGITSAPRFVIFDDGDRILKRLTFDQPTAMLAEQLK
                     HDPDLWDRQWVLDQLGGRPTDPDAAAAVADAAEHADHPFTRVTAATLLARFPAAAVLP
                     ALRAATNDTSALVRAAAVGAIGMLKDGGDSALAAARAAWTTDQSYGVRAAAVLALARL
                     APDERGATIRTALATPSYQDAIAMAGLRSLFLGPVDSTTLAAVEGKLGDIQSVSFALA
                     ALAGQGDAAAKAILDRHADDARPWVRDWVKRATTRR"
     gene            10135..10656
                     /locus_tag="VFU45_00120"
     CDS             10135..10656
                     /locus_tag="VFU45_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5357492.1"
                     /translation="MRAFVAQVGALDRAWLRGIAGWKAPRWLDRSIRAATHLGGATAT
                     VAIGAVLVVPAATRTLGLAVLVANALSHALVQLLKRSVLRERPGLADDGPVPLAPIPD
                     AYSFPSGHSAAALAVALPAALSGHPLGIPAVGLALLVGASRVYLRVHYVTDVLMGQIL
                     GALGAIAAVHLLA"
     gene            10756..10827
                     /locus_tag="VFU45_00125"
     tRNA            10756..10827
                     /locus_tag="VFU45_00125"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10788..10790,aa:Lys,seq:ttt)
     gene            10863..11717
                     /gene="ilvE"
                     /locus_tag="VFU45_00130"
     CDS             10863..11717
                     /gene="ilvE"
                     /locus_tag="VFU45_00130"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006185.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEU5357493.1"
                     /translation="MTTIWLDGKWCDRDTAMVSVFDHGLLYGDGIFEGIRVYGGRIFR
                     LAEHLDRLYDSAKAIWLTIPVAQDELARVHEEAARRSGLSEAYIRTVVTRGVGDLGLD
                     PRKCARPSVFVIVDKIALWPREKYEQGLVAVTAGTPVPHREALSPRVKSLNYLPHILA
                     KIEGIQAGADEVLMLESGGWVAEASGSNLFVVRRRVVQTPATASGILRGVTRDAVLEL
                     AAAAGYAVRETTLNRFDVYTADEVFFTGTGAEIIHARQVDGRLIGTGARGPVTTDLAE
                     RFHRLVRG"
     gene            11799..12038
                     /locus_tag="VFU45_00135"
     CDS             11799..12038
                     /locus_tag="VFU45_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357494.1"
                     /translation="MAWRVVELGDTVWNVTCAAERRAHTSSWQLILSFRSARPPKSSF
                     WAAYPLESTSKSSLFAQAERISHDRLTAVLAEHLR"
     gene            12047..12316
                     /locus_tag="VFU45_00140"
     CDS             12047..12316
                     /locus_tag="VFU45_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357495.1"
                     /translation="MTASMQDLRRVADALGSVRGQAVVEATVRPDLRQLRLAFAGGSI
                     LVVGAGSDDEGRGRLEVDIVRPAEEPTRQLEVPFEPGAVGGGGRP"
     gene            12313..12729
                     /locus_tag="VFU45_00145"
     CDS             12313..12729
                     /locus_tag="VFU45_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357496.1"
                     /translation="MSSLDMPERLRFRSFILERLPATRCKARVELAWGDDDSFVGESE
                     GLASPAGELRCAAEACVMALDRAVHGEVTLELLGVKAVKAFDNNVVIVSLAYQDKGAG
                     QRLVGCFLADVDSARGAALAVLNATNRVIGNRFFMR"
     gene            12845..14653
                     /locus_tag="VFU45_00150"
     CDS             12845..14653
                     /locus_tag="VFU45_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5357497.1"
                     /translation="MKPESRPSAALLLALLAALTGCAGSRPAPVAPAPKPAPPSSTGP
                     AASATAADPAPASLATSSAATGARTAGTADSGLSPADTAAADDSGATADEADSAADAA
                     ALEQLANASPGDSVDGVGGPAPTGGAKAIPAVTWDIDVNSFSNHERVQFYLDFFQGPA
                     RDRMAIWLQRMPKYEPMIRAAFAHEGLPGDLEYLALIESGFSNSATSRARAVGMWQFM
                     KGTGRMYGLRIDRWVDERRDPVKATPAAARYLRDLRDRFGSLYLAAAAYNAGGGRVSR
                     GISRLEDADGVDDESGSDSTFFRLYDTRYLRRETRDYVPKLIAAALIAKEPERYGFTP
                     APTVVPLTYDSLIVPDATGLDVIARLADTTIQAIRELNPEYLRMATPPRTASVVRIPS
                     GTGVQVAERYADLPASKRLTFREHFVERGETLGHIARVNHVTIEDIRLANPRIDPRRL
                     RLGSRLIIPVSGVAFDPRAAALAEASDAPRRRHAAPGGTHRVGSGESWWTLSRRYGVT
                     IAELREWNGASPTAGLRIGQWVKVAPAGRVRGRTRHAAAPSAVASAATHKVRYGETLS
                     ALAERYGVSISALRRFNALAPDEALRSGTVLRIPRG"
     gene            complement(14722..15396)
                     /locus_tag="VFU45_00155"
     CDS             complement(14722..15396)
                     /locus_tag="VFU45_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU5357498.1"
                     /translation="MRDRFDDGPARTPNLWLRGAGILVAAVIVSGLAGTLRGPEAAVA
                     SGVTLGPMPDVQGLEHQLNATRDALDVERMKNTRADAISSFSTAYQIPADLAAAIYDN
                     ALNAGIDPALGFQLVKVESGFRATARSSAGALGYTQLRLPTARTYEPWVTESTLVDRN
                     TNLRIGFRFLRELIGQYHGDVRLALLAYNRGPGRINEALAYGDDPDNGYSRAVLHHRP
                     ALPKKK"
     gene            complement(15393..15866)
                     /gene="moaC"
                     /locus_tag="VFU45_00160"
     CDS             complement(15393..15866)
                     /gene="moaC"
                     /locus_tag="VFU45_00160"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU5357499.1"
                     /translation="MKLSHLDDASRARMVDVGDKPVTARAAEAEGRVRMSAAAFALVR
                     DHAVAKGDVLAVAETAGTMGAKRTADLIPLCHPLGLDLVTVEATLDSELPGVLVRATT
                     KVVGKTGVEMEALTAVAVACLTVYDMTKAADRSAVIEEIRLVAKSGGKSGEWRKP"
     gene            complement(15863..>16334)
                     /locus_tag="VFU45_00165"
     CDS             complement(15863..>16334)
                     /locus_tag="VFU45_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357500.1"
                     /translation="LPDPEVRAFLRSQLSPAPSAAEIDARVARAGGVIGRAIDPSDDA
                     ARSRQAADALLSAIRGGPGARLERALSQGAFAARGDFTSLLDALAERLLETARTRLGH
                     PAPAGGLPAAVERTDAEGALRALERVRAARDAAQGNVNPQLILATLGAELAGAL"
BASE COUNT         2231 a         5850 c         5864 g         2389 t
ORIGIN      
        1 cccgttcacc gttcactgtt caccgttcac ctgaacaacg tgccccaccg gccccttccc
       61 gctcccgagg cccggtgccg cggcgatggc gcgggccacg aagtcgagtg cggtggtcac
      121 cgcgcgttcc agttcccagc cgagcgcgag cccggcagtg atcgccgccg agagcgtgca
      181 gccggtcccg tgggtcgagt tcgtggcgac ccggcggtga cggaagcgcc ggatgccggt
      241 ccgggtcacc agcacgtcga ccaggtcgtc gccggccagg tggccgccct tgacgagcac
      301 ggcgccggcg ccgagcgcca ggaggcgccg gcccgcgtcg tccatcgccg ccggatcccg
      361 cacgacgatc ccggtgaggg cctccgcttc gtcgagattc ggcgtcacca gttccgccag
      421 cggcagcaga cggtcgcgca cggtcgatgc ggcagccgcg tccagcagcc ggtggcccga
      481 tgtcgaggtc atcaccgggt cgagcacgta atggcgccag gcgcgtcccg cgatcgtctc
      541 ggccaccacg atggcgatgg cgccggtggc cagcatgccg gtcttgagcg cggcgggcgg
      601 gaggtcgtcg gccagggcgt cgagctgggc gcggaccagt cccgggtcga gcggatcgac
      661 acggcgcacc tccaccgtgt tctgggcggt gacggcggtg aggaccgacg tgccgtaggc
      721 cccgaattgg tggaacgtct tgaggtcggc ctggatgccg gccccgccgc cggagtcgga
      781 tccggcgacg gtgagcgcga tcggcaatag cgggggcatc gctacaatat atggacgcgc
      841 cgccgccctc cgtcccggtg gcgctgtcgg ggccacgttc ccacttcccc tttcccccgt
      901 cttcccgctt cttgaaggcg atgagcacac tcctttccac catccgggac ctccatcgcc
      961 gcaaggcgcg agagcgccgc gcgctggccc tggccgaggg gatccgcctg gtcgaggagg
     1021 cgctggccgc cggggtcgcg atcaaggggg cggccatcgc gccggcgctc gaggggacag
     1081 cgcggggcca ggcgctcaag gcagcgctca cggccaggtc cgtgccgctg gaagtggtca
     1141 ccgacgctga actcgtgggc cttgccgaca ccgagcaccc gcagggtgtg gtggcagtgg
     1201 cctcgcccag ggtgtggtcg ctggccgata tccacgtgac gccagggcac cccgtcctcg
     1261 tgctcgatgg cgtgcaggac cccggcaacg tcgggacgat cgcgcggacc gcgctggcgc
     1321 tcggcgcggc ggggctggtg gcgctccccg gcaccgccga cctcctcaac cccaaggcgt
     1381 tgcgcggatc gatgggcgcg ctcttccgcc tgccggcggt ccacgccgcc gatgccgaat
     1441 gcctggactg gctcacggcg ggcggcgtag cgctctgggt caccgacgcc aacggcgagg
     1501 acatccgccg gatcggaccg ccgaccgcgc cggtggccct ggtcctcggc aatgaaggcg
     1561 ccgggatccg cgccgacctc ctggccgcgg gcatgcggcg ggtgtcgatc ccgatcgcgc
     1621 aggaggcgga gtcgctcaac gtggccgtgg cggccggcat ccttctccac gaggtgaccc
     1681 gtggccggtg acccgaccct gaccctcttc ggcgcgttgc tcggcgccgt cctcggcagc
     1741 ttcctcaacg tctgcatcct gcgcctgccg gccgatgaat cggtggtgcg cccgccgtcg
     1801 cgctgcccca agtgcgggta cgcgctccgg tggttcgaca acatccccgt cctctcctgg
     1861 gtgctgctgc gcggaacgtg ccgccggtgc gccgcgccga tctcgatcca gtacccgctc
     1921 gtcgaactcg cgactgcgct catctgggcc ggcttcgcct gggcggccgg cccctcgctc
     1981 gaggcggtcc gcggcgcggt cttcttcacc atcctcctcg gcatcgcgat gaccgacgcc
     2041 cgggagtacg tcatccccga cgaattctcg ctcggcggcc tcatcctcgg cgtggcgctc
     2101 caccttcccg acggattcgg cgggatcggg cgatcgctcc tgggtgccgc ggtcgggttc
     2161 ggcgtcctct ggctcgtggc ggtggtgggg aagaaggtgt tcaaggaaga cgcgatgggg
     2221 ggcggcgaca tcaagatgat ggcgatggtg ggcgccttca ccgggtggac cggcgtgctg
     2281 ctcacgatct tcctcggttc ggtgctcggc gtgctcatct tcgtgccgcc ggcgatcctc
     2341 ggcaagaaga agctggtgcc cttcggcgtc ttcctggcgc ttggcgcggc cacgacctac
     2401 ctggtcggcg gcgccatcat cgactggtac cgccaggtcg cgttcggcgg atgaggcagc
     2461 gcgtcctcgt cgcggcggtc gtggcgctcg ccgcctgccg cgggcgtccg cccgcgacat
     2521 cggacgccgc tgagctcacc cgcctcatgg attccctggc gccgtacgtg gaacacgcca
     2581 cggggctccg tttcacccgc ctgccgcggg ccgagatcct ctcccgcgac cagctgcggg
     2641 cgtacctcga cgcccggttc cgcgaggaat acccgggcca gcggatgcgc gacctgcggg
     2701 acgcctatcg cctcctcacc ctccttcccg attccactga cctcggcaag ctctacctcg
     2761 acgtgctgag cgaacaggtc gccgggttct acgaccccga ctccgccgcc ttcttcggcg
     2821 ttgcgggtgc gccgccggcg ttccgggtgt ccaccgtcgc ccacgaactg gtccacgcgc
     2881 tccagcacga ctacatcccc ctcgattcca tcctgggcca gaaggcggac gccgaccggc
     2941 tgctggcggc gcaggcgatc ctcgagggcc aggcgaccat cgcgatgatg cggatgcagc
     3001 ccggggtcgg cgaccgggtg ctcgaccccg cgctctggga gacggtgcgg gaccagctcg
     3061 cgcagaacca gcggacgatg ccggtcttcg ccggcgcccc gcgggtggtg cgagaggggc
     3121 tcatcttccc gtacctgtca ggggccgagt tcatgcgctg gtggatgacc acccaccccg
     3181 attcggtcca gccgtacgga gcccggatgc ccaccagcac cgagcagatc ctcctgccga
     3241 gccattatct ggccggagac cggccggtcc cggtctcgtt caccgccggc ccccccgact
     3301 cggccgacac cgacgtcctc ggtgacgaag gggtgcggat cctcctcgcc gagatccggg
     3361 ggcaggccga agtgccgacg gccattccga tcggttgggg cggggaccgg tacgtcatct
     3421 atgaaacggc gcccggaccc gcgctcgtgt gggctgcggt ctgggacacg ccggtggccc
     3481 gggaggggtt cctggcggcg gccaaggggc cctggctcag gcgcgtgcgt cccggatacc
     3541 ggatgtcgtt ggaagcaaag gacatcgacg gccgccccgg gaccctgttc gtccacgccc
     3601 cgaccggctg gcccggatgg cgggacctgc cggtcgcaga agcacgctcg gcgccccgct
     3661 cgccgtctca agccccaagc gccaagccct aagcgccaag ccctaagccc taagccccga
     3721 gcgtactccc cgcccggttg ctcctgccac gtaaccccgc tataattcaa ggcttatgtt
     3781 cgacgagctc tccggaaaac tgactgccac gctccagaag ttgacggggc gcggggtcct
     3841 gaccgaagag gccgtccgcg agggtcttcg cgaggtccgc cgggtactgc tggaggcgga
     3901 cgtgagcttc gagctcacgc gcgagttcct cgagcgggtg caggagaagg ccgtcggggt
     3961 cgtggcgctc aagagcgtcc gccccgggca gcagatcgtc aagatcgttc acgacgaact
     4021 ggtggcgctt ctcggcgagc ggcaggcacc gctcgccttc gcgtcggtgc ccccgacggt
     4081 ggtcctgatc gtcggactgc agggttcggg caagacgacg acggcgggga agctcgccag
     4141 gcggctgaag ctcgagcaga aggcgccgtt cctcgtggcc gcggacgtct accgtcccgc
     4201 cgcggtggaa cagctggaaa cgttggggcg gcaggtgcag gtgggggtgc atgcggagcc
     4261 cggtgtcacc gacgtggtgg gcatcgtgcg gcgcggcatc gaggcggcgg ggaaggtgcg
     4321 ggcccgcacc gtcctggtgg acaccgccgg ccgcctgcag gtcgacgacg agctgatgga
     4381 tgagctccgc cgcctcaagg acgcggtcaa gccccacgag atcctcctcg tggccgacgg
     4441 gatgacgggc caggacgcag tgcgcatcgc gcgcggcttc cacgaggccc tcggcgtgac
     4501 gggggtggtg ctcaccaaga tggacggcga tgcccgcggc ggcgcggcgc tctccatcta
     4561 cggggtgacc cgggcgccga tcaagtacgt cggcacgggc gaagggctcg acgccctgga
     4621 gcccttccat cccgagcgga tggccgggcg gatcctccag cagggcgacg tcctgacgct
     4681 ggtggagaag gcgcaggggg tcgtcgacca ggccgaggcc gagcggctcg ccaagaaggc
     4741 ggtgtcgaag aaggggatgg acctccagga cttcctgggc gccatgaagc agatgcaggc
     4801 gctggggccg ctcaagggcg tgctggggat gctgccgggg gtcaatgccc aggcgctttc
     4861 caaggcgaac ctggacgaga agaagctcaa gcacgtcgag gccatcgtgc tctcgatgac
     4921 gccgaaggag cgcaccgacc cggacgtgat caacgggtcg cgccggctcc ggattgccaa
     4981 gggggcgggc cggccggtgc aggaagtgaa ccagttgctg gcgcagttcg gccagatgaa
     5041 gaagttcatg aagtcggcgg gaaagcccgg ggccaggatg cccttcgggc ctcgatcccc
     5101 cgcctgaggt ctcacgcaga ggagcaggga atggcaacga ggattcgtct ccgccgggtc
     5161 ggccgcaagg gcgtgccgct ctaccgcatc gtggtggccg agaaggaaaa ggcgcgcgat
     5221 ggccggttca tcgccacgct cggcgagtac aacccgaagg cgaagaccgc cgcggagaaa
     5281 gtgaaggtca acgtcgagca ggcgaaggcg tggctggcca agggcgccac gccgagcgac
     5341 accgtggcgg cgctgctcag ggccgccggc gtcgccgtcg cctcggcctg acgtggcggg
     5401 cgggtcgcgc cacctggtgg tggggcggct ccgcaagccg cacggcctga agggcgagta
     5461 cacggtgttc ccgctcaccg accggccgga ggaggtcttc gtcgccggac gccggctgga
     5521 gcgctgggac ctgggcgggg cggtgctggg cgaggtccag gtggagcggg ccaagggcta
     5581 tcaccgcgag tggctgctca agttccgcga cgtggaggac cgggtggccc tggatcagtg
     5641 gcgggggcag ttcctcgccg ccgcggtgga ggagctggcg ccgccggccg agggcgaggt
     5701 ctacctgcac gagctggccg ggttcagcgt gcgcggcccg gacggggtgc cggtggggct
     5761 ggtgagcggc gtctacgaac tgccgcaggg gctgatgatc gaagtgcagg ggccgaagcg
     5821 cgagttcctg ctgccgttcc gcaaggagtt cgtcacggcg gtggatcgcg cggagcggcg
     5881 gctgtcggtg gtggtcccgg acgggctggt ggagtgatgc tgcaggtcaa cgtggtgacg
     5941 ctcttcccgg aggcgttcgc cccctggctc tcggcgagca tcccggggcg ggcggcggcg
     6001 gcggggaagg tggggtaccg gctggtgcag ctccgggact tcacccatga ccggcaccac
     6061 accgtggacg atgcgccgta cggcggcggg gcggggatgg tgctcaagcc ggagcccttc
     6121 ttcgaggcgg tggagtcgtt ccggccctcg gggccgatcg tgctcctctc ggcccggggc
     6181 cggcggttcg ggcacaacga tgcggtgcgg tattcgctgg gcagcgagct caccctgctc
     6241 tgcgggcatt acaaggacgt ggacgaacgg gtggcggcgc acctggccac cgaggaagtg
     6301 tcggtgggcg acttcgtcct caccggcggc gagcccgcgg cgctctgcgt catcgatgcc
     6361 gtggtgcggc tcctccccgg cgcgctgggc gaccatgaat cggccagttc cgattcgcac
     6421 tacgacgggc tgctgagccc gccgagctac acccggcccc ccgagtaccg gggccaccga
     6481 gtgcccgagg tgctcctctc gggcgaccac gcggcgatcg cggcgtggcg cgcgcgggag
     6541 gcggagcgac tgacgcagga acggcggccc gacctctggg ccgcgtacga cgaggacacg
     6601 tgaaccgccc cccggggcga ccggcagcgc ccacggacgg cgcagaggac aggaccatgg
     6661 aaaaattggc gacagtggcg cgcgaaggcc tgcgtgcgga ccacccggcg ttcgacgccg
     6721 gcgacacggt caaggtgatg gtgcgcgtcc gcgaaggcga caaggagcgg ctgcaggcgt
     6781 tcgagggcct ctgcatcgcg aagcggggcg gcgggatcag cgagtcgttc acggtgcgga
     6841 aggtctccgc cggcgtgggc gtcgagcgca tcttcccgct gcatgcgccg acgatcgcga
     6901 gcatcgagct ggtccgcaag ggccgcgtgc gccgtgccaa gctctactac ctccgcgcgg
     6961 tgagcggcaa ggcggcgcgc atccgggaga agcgcgaggg ctgaccgtgc ccgccctgcg
     7021 gcccaccctc gagcgcgagc gcgcggcgtg ggcggcgggg aagttgctgg tcggcgtcga
     7081 cgaggtcggc cgtggccccc tggctggtcc agtggtggcc gcggccgtcg tcttttcggc
     7141 cggtgccatc ccgccggacg gcgtgcggga ctcgaagacc ctctccgctt cccgccgcga
     7201 cgccctgacc cctgccatcc gtgccgccgc gcttgccgtc gccatcggcg cggcgtccac
     7261 cgccgagatc gaccgctgga acatccgccg cgccaccgcg ctcgccatgc gccgcgcggt
     7321 cgcccgcctg cagcgggccc tcggcgccgt ggattgcctg ctcctcctcg acggcctgcc
     7381 gctgcccgag atcgggtatc cccaccaggc gctggtcgat ggcgacgcgc actgcatgag
     7441 catcgccgcc gccgggatcg tcgccaagac gctccgcgac cggctgatgg cccgcctcgc
     7501 ggcccggcac cccggctacg gctgggagac caacgccggc tacggcaccg ccgagcacct
     7561 ggcggcgctc cgcgagcggg gaccgacccg gcaccaccgg gtgagctttg cgccggtggc
     7621 ccagctgggg ttgtaggcgt taatttcagc tatcagctat cagctatcag ttgtcagttg
     7681 tcagttgtca gtccccccac ccccgatggg cccaatgcgc agactcctcg ccgttctcgt
     7741 cctcgcgttc ctcccggtga ccctcgccgc ccagagcaac gccgagcgga tcctcaacga
     7801 ccactacacc cgctcgcacg actatgacct ggtccaccag gacatcgagg tgcgggactt
     7861 cgactgggac tccctcgcgt tcaagggacg ggtggcgacg acgctcgtgg cgctcacgcc
     7921 ggggatggac tcggtggtgc tcgatgccgg ggcgctgctc catgtgtccc aggtgcgcgg
     7981 cgccgccggc ccgctcgact tcggccggcg cggcgacacg ctggtggtgc acctctccca
     8041 ctccgccgac ttcggtgaca cggtgcggtt cactgtgacg tacgacgcgc ggatctcgaa
     8101 cggacggggg ctcacgttcc tcaccggcca aggccgcccc catcgccccg accagatctg
     8161 gagccagggc gaggacatga acaaccactt ctggttcccg acctacgact tccccaacga
     8221 caaggcgacc tggaccctca ccgccgtggt gccggccagg gacatggcgg tctccaacgg
     8281 tcggctcgtg tccaacgtcg ccgcgcgggg cgtccgcacg gtccggtggt cggaggaccg
     8341 gccggcctcg acctacctcg tgtcgctcgt ggtggcgccc ctggtccgga tccacgacca
     8401 gtggcacggg atcccggtcg actactacgt ctaccccgag gacagcgcgc tcgcgcggcc
     8461 gctcttccgg gtgaccccgg acatgatcga cacctactcc cggctcaccg gggtcgccta
     8521 tccctggcag aagtacgccc agaccaccgt ggccgacttc ttcggcggga tggagaacgt
     8581 cagcgcgacg acgctggtcg actggctccc cgatgcccgc gcctacgccg accgcccgtg
     8641 gtaccagtac atcctgatcc cgcacgagct ggcccaccag tggttcggcg acctggtcac
     8701 cacggagaac tgggccaaca cctggctcaa cgagggcttt gccgagttca tgccggggca
     8761 gtactggggc accaggctcg gcgcccatgc ggagcaggac tactacgccg atgaataccg
     8821 gcagttcatg gccatcgacc accgcaagcg gatgccgctc gcggcgcagg ggtcgaacaa
     8881 catctacccc aagggcgcgc tcgtcctcga gatgctgaag ggctacctgg ggccggagcg
     8941 cttctgggcg tcgctgcacc gctacctgac gcgcaacgcc tacgacaacg ccgtcaccga
     9001 cgacctccgc caggcggtgc tcgatgccac cggggagaac ctggactggt tcttcgacga
     9061 gtgggtgtac caggcgggct atccggagtt caccgtcacc cagtcgtacg actcggcggc
     9121 gcatcgggtg acgctcgtgg tgctgcagac gcaggtcgac agctccaagc ccgattccgc
     9181 caccggcatc cgctacacca cgccggccgt gttccggatg cccgtgacgg tgcgcatcgg
     9241 caccgcggcg ggcgacgtga ccgcccgcga ctggatcgac gcgcgccagg acacgatcgt
     9301 ggtgtccggc atcacctcgg cgccccggtt cgtgatcttc gacgacggcg accggatcct
     9361 caagcgactc accttcgacc agcccaccgc gatgctggcg gaacagctca agcacgatcc
     9421 cgacctctgg gaccggcagt gggtgctcga ccagctgggc gggcggccca ccgatcccga
     9481 cgcggcggcg gcggtcgccg atgcggcgga gcacgccgac catcccttca cccgggtgac
     9541 ggcggccacc ctgctggcgc gcttccctgc cgccgccgtg ctccccgcgc tccgggcggc
     9601 gacgaacgac acgtcggcgc tggtgcgcgc ggcggcggtc ggcgccatcg gcatgctcaa
     9661 ggacggcggc gactcagccc tcgcggcggc gcgcgccgcc tggaccaccg accagagcta
     9721 cggcgtccgc gcggccgcgg tgttggcgct ggcccggctg gcaccggatg agcgcggggc
     9781 cacgatccgg acggcactcg cgacgccgtc gtaccaggac gccatcgcca tggcgggcct
     9841 gcggtcgctc ttcctggggc cggtcgactc caccaccctc gcagcggtgg aagggaagct
     9901 gggcgacatc cagagcgtct cgttcgcgct ggcggcgctg gccggccagg gggacgcagc
     9961 ggccaaggcc atcctcgacc gccacgccga cgatgcccgc ccctgggtcc gggactgggt
    10021 caagcgggcg acgacccggc gctagcccac cggtgttacc ccgccgttac cggcgccgga
    10081 tgacaacgtg acgcccgcgc gcgaccttcc cgtcacatgg tggaaggctt cgcgatgcgg
    10141 gcgttcgtcg cccaggtagg cgccctcgac cgggcgtggc tccgagggat cgccggttgg
    10201 aaggcgcccc ggtggctcga ccggagcatt cgcgcggcga cccacctcgg cggcgccacg
    10261 gcgacggtcg cgatcggcgc cgtgctcgtc gttcccgccg ccacccgcac gctcggcctg
    10321 gcggtcctgg tggcgaacgc cctgtcccac gcgctggtcc agctcctcaa gcgctcggtg
    10381 ctgcgtgagc gccccgggct cgccgacgat ggaccggtgc cgctcgcccc gatccccgac
    10441 gcctactcgt tcccctcggg ccactctgcg gccgcgctcg cagtggccct cccggccgcc
    10501 ctatccggac atccgctcgg gatcccggcc gtgggcctgg cgctcctggt cggcgcctca
    10561 cgggtgtatc tccgcgtcca ctacgtcacc gacgtgctga tgggccagat cctcggcgct
    10621 ctcggggcca ttgccgcggt gcacctgctc gcctgagcgc cgcggcccgg ccccgctcgt
    10681 tccgcaccgc tcccccccgc gttatcttcg gatcggacgt tcacgcgccg gtccgccagc
    10741 gcgtccgcca catccgcgcc cgtggtgaaa cggtatcaca tcggcctttt aagccgctag
    10801 ttcctggttc gagtccaggc gggcgcatcg cagtccacgt cacttttccg acaggcacgc
    10861 gcgtgaccac catctggctc gacgggaagt ggtgcgaccg ggacaccgcg atggtgtcgg
    10921 tgttcgacca tggccttctc tatggcgacg ggatcttcga ggggatccgg gtgtacggcg
    10981 gcaggatctt ccggctcgcc gagcacctcg accggctgta cgattcggcc aaggccatct
    11041 ggctcacgat cccggtcgcg caggatgagc tcgcccgggt ccacgaggag gcggcgcgcc
    11101 gttcgggcct ctccgaggcc tacatccgca ccgtcgtgac gcgcggcgtg ggcgacctgg
    11161 ggctcgatcc acggaagtgc gcccggccgt cggtgttcgt gatcgtcgac aagatcgcgc
    11221 tctggccgcg ggagaagtac gagcagggac tggtggcggt caccgccggc accccggtgc
    11281 cgcatcgtga ggcgctctcc ccccgggtca agtcgctcaa ctacctgccg cacatcctgg
    11341 ccaagatcga gggcatccag gccggcgccg acgaggtcct gatgctcgag tcgggcggat
    11401 gggtggccga ggcgtcgggg tcgaacctct tcgtcgtgcg gcggcgggtg gtgcagacgc
    11461 cggccacggc atcggggatc ctccgcgggg tgacccgcga cgccgtgctc gagctggccg
    11521 cagcggcggg gtacgccgtg cgcgagacga cgctcaatcg gttcgacgtg tacacggccg
    11581 atgaggtctt cttcaccggg accggtgccg agatcatcca cgcgcggcag gtcgacggcc
    11641 ggctgatcgg caccggcgca cgcggccccg tcaccaccga cctcgcggag cggttccacc
    11701 ggctggtgag gggctgacgc accgctcttg accggcctct ccgctgaccg gcatattgcg
    11761 aaggtccata ccccaacgcc cagggagact gcatcgcaat ggcgtggcgc gtcgtcgaac
    11821 tgggagacac cgtctggaac gtgacctgtg cggccgagcg ccgggcgcac acctcgtcgt
    11881 ggcagttgat cctctccttc cggtcggcgc gtccgccgaa gtcctccttc tgggccgcct
    11941 atccccttga atcgacgtcg aagtcgagcc tcttcgccca agcggagcgg atctcgcacg
    12001 atcgtctgac ggcggtcctc gccgagcacc tgcgctgagg gtcgacatga cggcctcgat
    12061 gcaggatctc cggcgggtcg ccgacgccct cggctcggtg cggggacagg ccgtcgtcga
    12121 ggcgaccgtc cggcccgacc ttcggcagct ccggctcgcg ttcgccggcg ggagcatcct
    12181 ggtggtgggc gcgggcagcg acgacgaggg gcgcggccgg ctggaggtcg acatcgtacg
    12241 gcccgccgag gagccgaccc gtcagctcga ggtgccgttc gagcccggcg cggtgggcgg
    12301 cggagggcgg ccgtgagcag cttggacatg ccggagcgcc tgcgcttccg cagcttcatc
    12361 ctggagcggc tgcccgccac ccgctgcaag gcccgggtcg agctggcttg gggcgacgac
    12421 gactcgttcg tgggcgagag cgaaggcctt gcctcgccgg ccggggagct gcgctgcgcc
    12481 gccgaggcgt gcgtcatggc gctcgaccgc gccgtccacg gcgaggtgac gctcgaactc
    12541 ctcggggtca aggcggtcaa ggcgttcgac aacaacgtcg tgatcgtgtc gctcgcgtac
    12601 caggacaagg gcgccggcca gcgactggtc gggtgcttcc tggccgacgt cgactcggcc
    12661 cgcggcgcgg cactcgccgt cctcaacgcc accaatcgcg tcatcggcaa ccgcttcttc
    12721 atgcggtgac gctccgaaga tcgaccgaaa ccatttggtc tcgccggcta acttcttaca
    12781 tttcaagcgt tccccgtggt ccggccagcg gccggtcccg gctgcggcct tccaaggagc
    12841 gcgtttgaag cctgagtccc gaccgagcgc cgcgctcctg ctggcgctcc ttgccgccct
    12901 caccggatgt gccggcagtc gtccggcgcc ggtggccccg gcgcccaagc cagccccccc
    12961 atccagcacc ggccccgccg cctcggcgac ggccgcggac ccggcgcctg catcactcgc
    13021 cacgtcgtcc gctgccaccg gggcccggac cgccggcacc gctgacagcg gcctgtcgcc
    13081 ggcggacact gccgcggccg atgattccgg cgccaccgcc gacgaggccg actccgctgc
    13141 ggacgcggcg gcgctggagc agctggccaa cgcgagcccc ggggattcgg tcgacggcgt
    13201 cggcgggccg gcgccgacgg gcggcgccaa ggcgatcccg gcggtgacct gggacatcga
    13261 cgtcaactcg ttctcgaacc acgaacgggt ccagttctac ctcgacttct tccaggggcc
    13321 ggcccgcgac cggatggcca tctggctgca gcggatgccc aagtacgagc cgatgatccg
    13381 cgcggcgttc gcccacgagg ggctcccggg cgacctggag tatctcgccc tgatcgagag
    13441 cggcttttcc aacagcgcca ccagccgggc gcgtgcggtc gggatgtggc agttcatgaa
    13501 gggcaccggc cggatgtacg ggctccggat cgaccggtgg gtcgacgagc gccgcgaccc
    13561 ggtcaaggcg accccggcgg ccgcccgcta cctccgtgac ctgcgtgacc ggttcggctc
    13621 gctctacctg gcggcggcag cttacaacgc cggcggcggc cgggtctcac gggggatctc
    13681 gcggctcgag gacgccgacg gcgtggacga cgagtcgggc agcgattcca ccttcttccg
    13741 tctctacgac acccgctacc tccggcgcga gacccgcgac tacgtaccga agctgatcgc
    13801 ggcggcgctc atcgcgaagg agccggagcg gtacggcttc acgccggcgc ccacggtggt
    13861 gcccctcacc tacgactcgc tcatcgtgcc ggacgccacc ggactcgacg tgatcgcccg
    13921 cctggccgac accacgatcc aggccatccg cgaactcaac cccgagtacc tccggatggc
    13981 gacgccgccc cggaccgcgt cggtggtgcg gatcccgtcg ggcaccggcg tgcaggtcgc
    14041 ggaacgctac gctgacctgc ccgcctcgaa gcggctcacc ttccgggagc atttcgtgga
    14101 gcggggcgag acgctggggc acatcgcgcg ggtgaatcac gtgacgatcg aggacatccg
    14161 gctggccaat ccgcggatcg acccgcggcg gctgcggctc ggctcgcggc tcatcatccc
    14221 ggtgtccggg gtggcgttcg acccccgcgc ggccgcgctg gccgaggcgt cggatgcgcc
    14281 ccgccggcgc catgcggcgc cgggcggcac ccaccgggtc gggtcgggcg aatcgtggtg
    14341 gacgctctcc cgccgttacg gcgtcaccat cgccgagctt cgtgagtgga acggtgcctc
    14401 gccgacggcc gggctccgga tcgggcagtg ggtcaaggtc gccccggccg ggcgcgtgcg
    14461 cggccggacg cgccacgccg cggctccgtc ggccgtggcg tcggccgcca cccacaaggt
    14521 gcgctacggt gagacgctct ccgccctggc cgagcgctac ggcgtgagca tctcggcgct
    14581 ccgccggttc aacgcgctgg cccccgacga ggcgctccgg tcgggcacgg tgctgcggat
    14641 cccccgaggc tgagcgcggc gggtcccacg aacgacgaac ccccgtccag ggccggacgg
    14701 gggttcgtca ttccgggtgg gtcacttctt cttcgggagc gcgggacggt ggtggagcac
    14761 cgcgcgggag tacccgttgt ccgggtcgtc gccgtaggcg agggcctcat tgatccggcc
    14821 cgggccgcga ttgtaagcga ggagcgccag gcgcacgtca ccgtggtact ggccgatcag
    14881 ctcgcgcagg aagcggaacc cgatccggag gttggtgttc cgatcgacca aggtcgactc
    14941 cgtgacccac ggctcgtacg tccgcgcggt gggaaggcgg agctgggtat agccgagggc
    15001 gccggccgag ctccgggcgg tggcccggaa tccgctctcc accttgacca gctggaagcc
    15061 cagggccggg tcgatcccgg cgttgagcgc gttgtcgtag atcgccgcgg ccaggtcggc
    15121 cgggatctgg tatgcggtcg agaagctgga gatcgcatcg gcgcgggtgt tcttcatccg
    15181 ctccacgtcg agcgcgtcgc gggtggcgtt gagctggtgc tccaggccct gcacgtccgg
    15241 catcggaccc agggtcacgc cggacgccac tgcggcctcg gggccgcgga gggtccccgc
    15301 gagcccgctc acgatcaccg ccgcgaccag gatgcccgcg ccgcggagcc agaggttggg
    15361 ggtccgggcc gggccgtcat cgaaccggtc tctcatggct tcctccactc accgctcttg
    15421 ccgccggact tcgcgaccag ccggatctcc tcgatcacgg ccgaccggtc cgcggccttg
    15481 gtcatgtcat acacggtgag gcacgccacc gcgacggcgg tgagcgcctc catttccacc
    15541 ccggtcttcc cgacgacctt ggtcgtggcc cgcaccagga cgccggggag ctccgagtcg
    15601 agcgtcgctt cgacggtcac caggtcgagg ccgagcggat ggcagagcgg gatcaggtcc
    15661 gccgtccgct tggcgcccat cgtaccggcc gtctccgcca ccgcgagcac gtctcccttg
    15721 gccaccgcgt ggtcccgcac cagcgcgaat gccgcggccg acatccggac ccggccttcg
    15781 gcctcggccg cgcgcgcggt gaccggcttg tcgccgacat cgaccatccg ggcgcggctt
    15841 gcgtcgtcga ggtggctcag cttcacaggg cccccgcgag ttcggcgccc agtgtggcca
    15901 ggatgagctg ggggttcacg ttgccctggg ccgcatcgcg ggccgcgcgg acacgctcca
    15961 gggcgcggag ggcaccctcg gcatccgtcc gctcgaccgc ggcgggtagt ccgcccgcag
    16021 gtgccggatg gccgaggcgg gtgcgggccg tctcgagcag ccgctcggcc agggcgtcga
    16081 gcagggaggt gaagtccccg cgtgcggcga aggcgccctg cgagagtgcg cgctcgagcc
    16141 gggccccggg gccgccacgg atggcggaga gcagcgcatc ggccgcctgc cggctccggg
    16201 ccgcgtcgtc ggacggatcg atcgcgcggc cgatgacacc gccggcccgt gctacccgtg
    16261 cgtcgatctc ggccgcggat ggcgcggggg agagctggga ccggaggaac gcccggacct
    16321 cggggtcggg gagc
//
LOCUS       DASUAP010000004         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1117573, whole genome shotgun sequence.
ACCESSION   DASUAP010000004 DASUAP010000000
VERSION     DASUAP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117573"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFU45_00170"
     CDS             <1..874
                     /locus_tag="VFU45_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357501.1"
                     /translation="GGTTIPRLLEALGCEVVAINLETDGRFPRAPEPIPENLGDLRRL
                     VRESKADIGMAVDPDVDRLALVDETGHPIGEDYTLAFAVRAVLGGRTIGQAHGRTERG
                     KSASGPTVRPSAGAPTVVANLSTSRVVEDAAAAFGARFLRAPVGEANVARMIRDEHAV
                     IGGEGNGGVILPAVHIGRDAPVGVALILELLRREGRPVSGIVSEAPQYIIVKAKAPRG
                     PDLAPVYDGLRRRFPEAAADTRDGLRLAWADRWVHARPSGTEPIVRFIAEAPTRADAE
                     ALIEACRGIFQQGN"
     gene            881..2707
                     /gene="glmS"
                     /locus_tag="VFU45_00175"
     CDS             881..2707
                     /gene="glmS"
                     /locus_tag="VFU45_00175"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5357502.1"
                     /translation="MCGIVGYIGGREAAGLLIDGLRRLEYRGYDSAGIAVVSGGRVDV
                     RKAAGKLVNLERAIADDMPRGTLGIGHTRWATHGAPTTPNAHPHTDQSGKIALIHNGI
                     IENAGSIRKLLLQRGHTFTSETDTEVLAHLVGEHYRGKLAEAVAAALREVEGAYGIAV
                     ISAAEPDVLVAARQGSPLLVGVGDHEWFVASDASAILSHTRSVVYLDDGEMAVLTREG
                     YSVRDLALSRISKPVNQIDWDLATIERGGYDHFMLKEIFEQPETIRNTMRGHLLEEQG
                     TARLDGLNLTDEELKKFDRIVITACGTSWHSAIIGEYMIEELARVPVEVEYASEFRYK
                     NPLADERTLVIGISQSGETADTLAAIREAKQRGARTLGLVNVVGSTIAREVHGGTYLH
                     AGPEVGVASTKAFTSQVTALAMFALRLARLRNMSILQGRQFIEALQKVPDQVARILER
                     AVDVEKLAARFEHAGNALYLGRGVNFPVALEGALKLKEISYIHAEGYPAAEMKHGPIA
                     LIDENMPVVFIAPKDGVHQKIMSNIEEVKARGGRVIAIVTEGDEEIARIADATFPIPE
                     TIDLLTPLLTIIPLQLFAYYVAVKRGCNVDQPRNLAKSVTVE"
BASE COUNT          477 a          984 c         1037 g          441 t
ORIGIN      
        1 ggggggcacc accatccccc ggctcctcga ggcgctcggc tgcgaggtcg tggccatcaa
       61 cctcgagacc gacggccgct tcccccgcgc ccccgagccg atcccggaga acctgggtga
      121 cctccgccgg ctggtccggg aatccaaggc cgacatcggg atggcggtcg atcccgacgt
      181 ggaccggctg gcgctcgtcg acgagaccgg ccatccgatc ggtgaggact acacgcttgc
      241 gttcgcggtg cgggcggtgc tgggtggtcg gacgatcgga caggcgcacg ggcggaccga
      301 acggggcaag tcagcgtccg gtccgactgt gcgtccatcc gccggtgcgc ctaccgtcgt
      361 cgcgaacctt tcgacctcgc gcgtggtgga ggacgcggcg gcggcgttcg gggcccggtt
      421 cctccgtgca ccggtgggcg aggccaacgt ggcccggatg atccgggacg agcacgccgt
      481 catcgggggc gaggggaatg gcggggtgat cctgccggcg gtccacatcg gccgcgacgc
      541 gccggtgggg gtggcgctca tcctcgagct cctccggcgg gaagggcggc ctgtgtccgg
      601 catcgtgtcg gaagcaccac agtacattat tgtgaaggcc aaggctcccc gggggcccga
      661 cctggcgccg gtgtacgacg ggctccggcg gcggttcccc gaggcggccg cggacacgcg
      721 cgatggcctg cggcttgctt gggccgaccg ctgggtccac gcccggccct cggggaccga
      781 gccgatcgtg cggttcatcg cggaagcgcc gaccagggcg gacgcggagg ccctcatcga
      841 ggcgtgccgg ggcatcttcc agcaagggaa ctgacgacgc atgtgcggca tcgtggggta
      901 tatcggcggg cgtgaggcgg cggggctcct catcgacggg ctccggcgcc tggagtaccg
      961 cggctacgac tcggccggga tcgcagtggt gagcggcggc cgggtggatg tgcggaaggc
     1021 ggccgggaag ctggtcaacc tggagcgggc catcgccgac gacatgcccc ggggcacgct
     1081 cggcatcggc cacacccggt gggccaccca cggcgcgccc accaccccca acgcgcaccc
     1141 gcacaccgac cagtccggga agatcgccct catccacaac gggatcatcg agaacgccgg
     1201 gagcattcgg aagctgctcc tgcagcgcgg ccacacgttc acctcagaga ccgacaccga
     1261 ggtgctggca cacctggtgg gcgagcacta ccggggcaag ctggcagagg cggtggcggc
     1321 cgcgctccgg gaggtcgagg gcgcctacgg catcgccgtc atcagcgccg cggagcccga
     1381 tgtgctggtg gcggcccgcc agggctcgcc gctcctcgtg ggcgtgggcg accatgagtg
     1441 gttcgtggcg tcggacgcct cggccatcct gtcccacacc cggtcggtgg tgtacctgga
     1501 tgacggcgag atggccgtgc tcacccgcga aggctactcc gtgcgcgacc tggcgctctc
     1561 gcggatcagc aagccggtca accagatcga ctgggacctg gccaccatcg agcggggcgg
     1621 gtacgaccac ttcatgctga aggagatctt cgagcagccc gagaccatcc gcaacacgat
     1681 gcggggccac ctgctcgagg agcaggggac ggcgcggctc gacggcctca acctgaccga
     1741 cgaggagctc aagaagttcg accggatcgt catcacggcg tgcggcacct cctggcactc
     1801 ggccatcatc ggcgagtaca tgatcgagga gctggcccgg gtgccggtcg aggtggagta
     1861 cgcctccgag ttccgctaca agaacccgct cgccgacgaa cgcacgctgg tcatcggcat
     1921 ctcccagtcg ggcgagaccg ccgacaccct ggccgcgatc cgcgaggcca agcagcgcgg
     1981 ggcccggacc ctggggctgg tcaacgtggt gggttcgacc atcgcgcgcg aggtgcacgg
     2041 cgggacgtac ctccacgccg ggcccgaggt cggggtcgcg agcacgaagg cgttcaccag
     2101 ccaggtcacc gcgctcgcga tgttcgccct ccggctggcg cggctccgga acatgagcat
     2161 cctgcagggg cgccagttca tcgaagcgct gcagaaggtg ccggaccagg tggccaggat
     2221 cctcgagcgg gcggtcgacg tcgagaagct ggcagcgcgc ttcgagcacg ctgggaacgc
     2281 gctctacctg ggccgcggcg tcaacttccc ggtggccctc gaaggggcgc tcaagctgaa
     2341 ggagatctcc tacatccacg ccgaggggta cccggcggcg gagatgaagc acgggccgat
     2401 cgccctcatc gacgagaaca tgccggtggt gttcatcgcg cccaaggacg gcgtgcacca
     2461 gaagatcatg tcgaacatcg aggaggtgaa ggcgcggggc ggtcgggtca tcgccatcgt
     2521 gaccgaaggg gacgaggaga tcgcccggat cgccgatgcc accttcccga tcccggagac
     2581 gatcgacctg ctgacgccgc tcctcacgat catcccgctg cagctcttcg cctattacgt
     2641 ggcggtcaag cgggggtgca acgtggacca gccgaggaac ctggcgaaga gcgtgacggt
     2701 ggagtgatcg tgagacgtaa gacgtaagac gtgagacgta agacgtaaga cgtgagaggt
     2761 tggaggaaag ggaagaggga ataggacgga ggagaggggg agcccggtcc cttctccttc
     2821 tccttctcct tctccctcct tctccttctc cttttccttc tccttcttct ccctctattc
     2881 cctttcccct cttcactccc cttctcactt ctcacttctc acttcttact tatcacccg
//
LOCUS       DASUAP010000005         8084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_422576, whole genome shotgun sequence.
ACCESSION   DASUAP010000005 DASUAP010000000
VERSION     DASUAP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422576"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFU45_00180"
     CDS             complement(<1..722)
                     /locus_tag="VFU45_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5357503.1"
                     /translation="MTDRPPESAYDPQAVEAKWRARWAERHTNEPDLDRPVDPFYNLM
                     MFPYPSAEGLHVGNMFAFTGADVYGRYQRLRGRTVFEPIGYDAFGIHSENYALRIGEH
                     PGVVIPRNIANFRRQLERIGGMFDWRHTVDTTDPAYYRWTQWVFLELYRAGKAFKKKA
                     AVNWCPNDKTVLANEQVEGGRCERCGAVVEQRVLEQWFFRITDYAGRLLDHLDGLDWS
                     DTTRTAQRNWIGRSQGAEVDFDV"
     gene            843..2855
                     /locus_tag="VFU45_00185"
     CDS             843..2855
                     /locus_tag="VFU45_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5357504.1"
                     /translation="MAATDRGLRRKLYLFGGGVILAITVVAALLVLTTAAGLVSRQTD
                     AELATYASRTARMVQTTDEERRHEADLLALMPLTEQLAATGKADKGVDAVQALLRLRT
                     RTSFRDLAIYGPDGHLVITTAAHDSSGAAGKDWFRAALSSGTTQTAPYRSSDGQVLDI
                     ATPIRVEGQRAPAGVLRATLPLEALGRPLRRDLRVDSLTLVEVVDQHGLVAVTGIPGG
                     EVGQPFSDDSLLGRSTELHFARVKDTRGTERLAIVGIAAPAAWTVVARQPASAGLRIF
                     QGTWREMVIKALIFTLLALLLLAWLATWIDRTVLTPLKQAEGIASRVALGDLHQVDGL
                     RHDGTDEVSRLGRALRTMVTGLRELVGSIRTSAGESVDIATSISATTEQMNAATIEVS
                     GTCNDMTQRATVQADLVRETQQGAERIRAIAEQLATGARGAAERNSALARMAQGSRSR
                     LSQSAADLEALAEEVRLGTAEAAALAQASAEIQLFVAQAKSIAAQTHMLALNAGIEAA
                     RAGEHGRGFAVVADEVRKLARQAEASATTTTRTVQAVLARVEGTRERLARLATGGEAA
                     RTAAQRSAEEMEKLASQAEESDAWTREISGSAGDVHGLVETMSERMRGITGSTEEFAA
                     AAQQISASTQELSASTQEVAATAQLLAGAAERLSTAVKVFDLGGSA"
     gene            complement(2918..3535)
                     /locus_tag="VFU45_00190"
     CDS             complement(2918..3535)
                     /locus_tag="VFU45_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357505.1"
                     /translation="MSALTVVGIACAAVALVCLVLARRALRKRRPFATLTRALLAALF
                     VSLAAIAALVAVGTRGYRALTDEELAATVRVTPEGPHRFSAAFTFADGRSATYDIDGD
                     QLSVDARVLKWHYWADLLGLHTLYALDRVGGRYADLNDELHRPRTLYALGPERPLDLF
                     KLIREYSVLAPLADAEYGSGTFLDVTAPTTLDLRVSTTGLLLRKH"
     gene            complement(3532..4497)
                     /locus_tag="VFU45_00195"
     CDS             complement(3532..4497)
                     /locus_tag="VFU45_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5357506.1"
                     /translation="MRRAVALAALLLPAALVAQAPTDTSFADSLAVPGGILRGTLATP
                     TTPGPWPAVLFISGSGPTDRDGNSPLLAGRNNSLRYLADALAARGIASVRYDKRGIAA
                     SNGFALREADLRFDDYVGDAATWIRALKADRRFRSVTVVGHSEGSLVGMLAAAEAGAD
                     AFVSIAGAGRPIAQILHEQLAPQLPPDLLRQADSIMARLGTGERVDSVPPRLWMLYRQ
                     SVQGYLISWMRHDPARDLAALKVPVLIAQGTTDIQVDTLDARLLAAADPAARLLLIPG
                     MNHVLKMVPADRAAQVASYSDPALPVAPALVYGIATLVLGLGRAR"
     gene            complement(4494..5132)
                     /locus_tag="VFU45_00200"
     CDS             complement(4494..5132)
                     /locus_tag="VFU45_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU5357507.1"
                     /translation="MAATIFWDVDTQHDFMRPDGKLYVPGAEAIAPLLRALTDYAHRE
                     GIRIVASADDHVPGHRELSTAPDFLTTFPDHCMRGTPGQRKVAETALRDPLVIEPEVD
                     PGVAARVRAHRGDILLHKHWFDVFTNENVPAVVDALAPDRIVLYGVATDVCDKYAIEG
                     LRRRYPAIELWFVTDAARAIHPERTDLLLAEWRAAGVRLTDSAAVLRGGVPR"
     gene            complement(5282..6343)
                     /locus_tag="VFU45_00205"
     CDS             complement(5282..6343)
                     /locus_tag="VFU45_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357508.1"
                     /translation="MLARRPRLDPAIFNLPVEKMRAGYYSDKYFVRAREILLADGTHP
                     RVTMQVFGKSHAFLGGIDEAVAILKLCAVEWPALTVHALHEGDEVEPWETVMLIEGPY
                     DAFAHLETLYLGVLARRTRVGTNTRRVVEAAHPKEVMFFPARHDHWLVQTGDGYAAHV
                     AGAIGVSTDAQASWWGSEGVGTVPHALIAAYGGDTVLATRKFAEYIPSSVQVVTLADF
                     ENDCVRTSVECARALGSRLYGVRLDTSEMLVDRSLWDSMGRFKPNGVNPQLVRNVREA
                     LDREGFQGVRIIVSGGFTVEKIREFEAQQVPVDAYGVGSSLFQGRYDFTADVVLREGK
                     PCAKVGREYRPNPRLERVE"
     gene            complement(6344..6988)
                     /locus_tag="VFU45_00210"
     CDS             complement(6344..6988)
                     /locus_tag="VFU45_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357509.1"
                     /translation="MSLLDGSDPLDVLRAVLRILLVGATLVSLIVWLALGEPRMLQLA
                     TATWAIYGVMRAFVNGFLAPVAELPAAIGDDLRHDAPGSGSQVGALTATEQFRAAEAR
                     YRAPALPSADVDAAIQQAEAGAGPPGGAAAAVGALEALRAAHRLNADDDVRVGLLLVR
                     LHEEALHDPAAALREFRRLVFRHPDPPQVTRIRGALADISSRFLPAEPAENQAP"
     gene            7116..>8084
                     /locus_tag="VFU45_00215"
     CDS             7116..>8084
                     /locus_tag="VFU45_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEU5357510.1"
                     /translation="MPDSVSRREFLAATGAVVAASGATHPVPGFPAVHIRPPAKAVAV
                     SSANGFRGVQKAVDLMITGTDTLDAAIEGVKIQELDPNDHSVGYGGLPNEDGVVQLDA
                     SCMHGPTRRAGAVGALEGIKLASEIARLVLKYTDHIMLVGEGAKKFALSYGYQEEDLL
                     TPEARQIWLTWRANRGNKDDWMNVPDGTPMVHRPTGTINMDVVNALGEISSVTTTAGL
                     AFKIPGRVGDSPIIGAGQYTDNDVGAAGSTGLGELNIVACGAFFTVDGMRRGLKPTDA
                     AIETLKRVMQLAPPSLVAANGKPKFQLNYYAVNKKGEYGSAALYPSK"
BASE COUNT         1240 a         2766 c         2963 g         1115 t
ORIGIN      
        1 acgtcgaagt ccacctcggc gccctgcgac cgcccgatcc agttgcgctg ggccgtgcgg
       61 gtggtgtcgg accagtcgag cccgtcgaga tggtcgagca ggcgccccgc gtagtcggtg
      121 atccggaaga accactgctc cagcacccgc tgttccacca ccgccccgca ccgctcacac
      181 cgccccccct ccacctgctc gttggccagg acggtcttgt cgttggggca ccagttgacc
      241 gccgccttct tcttgaacgc cttccccgcc cggtagagct cgaggaagac ccactgggtc
      301 cagcgatagt acgccgggtc cgtggtgtcc acggtatggc gccagtcgaa catcccgccg
      361 atccgctcga gctggcggcg gaagttggcg atgttccgcg gaatgacgac ccccggatgc
      421 tcgccgatcc ggagcgcgta gttctcgctg tggatgccga aggcgtcgta cccgatgggc
      481 tcgaagaccg tccgcccgcg gaggcgctgg tagcgcccgt acacgtcggc cccggtgaac
      541 gcgaacatgt tccccacgtg cagcccctcg gccgagggat aggggaacat catcaggttg
      601 tagaacgggt ccaccggccg gtcgaggtcg ggctcgttgg tgtggcgttc cgcccaacgg
      661 gcgcgccact tggcctcgac ggcctgcgga tcgtatgcgg actcgggcgg acggtcggtc
      721 atgggagggg gaattgtaac gattttgggc gtgacgcggc acctttgact ttgccgcacc
      781 actcttgcag attgcgcgaa ccctgagccc ctcaatgaga catatcagcc agttccatat
      841 ccgtggccgc gactgatcgc ggcctgaggc ggaagctcta cctcttcggt ggcggcgtga
      901 tcctggccat caccgtggtc gcggcgttgc tcgtcctgac gacggccgcc gggctggtga
      961 gccgccagac cgacgcggag ctggccacct acgcgtcgcg gaccgcgcgg atggtccaga
     1021 ccacggacga ggagcgccgc cacgaggcgg acctgctggc gctgatgccg ctcaccgagc
     1081 agctcgccgc gaccggcaag gccgacaagg gcgtagatgc tgtccaggcg ctcctgcggc
     1141 tcaggacccg caccagcttc cgcgacctgg ccatctacgg cccggacggc cacctcgtca
     1201 tcacgacggc ggcccacgac tcctccggcg ccgcggggaa ggactggttc cgggccgcac
     1261 tctcctccgg cacgacgcag accgcgccat accgctcctc cgacggacag gtcctcgaca
     1321 tcgcgacgcc gatccgggtc gaggggcagc gcgcccccgc cggcgtgctc cgggcgaccc
     1381 tcccgctcga ggcgctgggc cgcccgctcc ggcgcgacct ccgggtcgac tcgctcaccc
     1441 tggtcgaggt ggtggaccag cacgggctgg tggcggtcac cgggatcccg ggcggagagg
     1501 tgggacagcc cttctccgac gattccctgc tgggtcgatc gaccgagctt cacttcgcgc
     1561 gcgtcaagga cacccgcggt accgaacggc tggccatcgt gggcatcgcg gcgccggcgg
     1621 cgtggacggt agtggcgcga cagccggcat cggcggggct ccgcatcttc cagggcacct
     1681 ggcgcgagat ggtcatcaag gcgctgatct tcaccctcct agcgctcctc ctcctggcgt
     1741 ggctcgccac ctggatcgat cgcacggtgc tcacccccct caagcaggcc gaggggatcg
     1801 catcgcgggt ggcgctgggc gacctgcacc aggtcgacgg gctccggcac gacggcaccg
     1861 acgaagtgtc gcgcctgggc cgggcgctgc gcacgatggt cacaggcctg cgtgaactgg
     1921 tcggctcgat ccggacctcc gccggggagt cggtggacat cgccacgtcg atctcggcga
     1981 ccacggagca gatgaacgcc gcgacgatcg aggtatccgg cacctgcaac gacatgaccc
     2041 agcgcgccac ggtccaggcc gacctcgtgc gggagacgca gcagggcgcc gaacggatcc
     2101 gggccatcgc cgaacagctg gccaccggcg cccggggggc cgctgagcgc aactcggccc
     2161 tggcgcggat ggcccagggc agccggagcc ggctgagcca gagcgcggcg gacctcgagg
     2221 cgctggccga ggaggtgcgg ctcggcaccg cggaagccgc tgcgctggcc caggcctcgg
     2281 ccgagatcca gctctttgtg gcgcaggcca agtcgatcgc ggcgcagaca cacatgctgg
     2341 cgctcaacgc cgggatcgag gcggcccgcg ccggtgagca cggccgcggc ttcgcggtcg
     2401 tggccgacga ggtccgcaag ctcgcccgcc aggccgaggc ctcggccacg acgaccaccc
     2461 gcacggtgca ggcggtgctg gcgcgggtgg aagggacccg cgagcggctg gcccggctcg
     2521 ccaccggcgg cgaggccgcc cggacggcgg cacagcgttc ggcagaggaa atggaaaagc
     2581 tggcgtcgca ggccgaggag tccgatgcgt ggacccgcga gatctcgggc tccgcgggcg
     2641 acgtgcacgg cctggtggag acgatgtcgg agcggatgcg cgggatcacc ggctcgacgg
     2701 aggaattcgc ggccgcggcc cagcagatct ccgcctcgac ccaggagctg agcgcctcca
     2761 cccaggaggt ggcggccacg gcccagctcc tcgcgggggc ggcggagcga ctctcgacgg
     2821 cggtgaaggt gttcgacctg ggggggagtg cgtagcagct atcagctatc agctatcagc
     2881 gcgaagataa cgcccggccc actgtgtgag ccgggcgtca gtgcttccgg aggaggagtc
     2941 cggtcgtcga gacgcggagg tcgagcgtgg tgggtgcggt cacgtcgagg aaggtcccgc
     3001 tgccgtattc ggcatcggcc agcggcgcca gcacgctgta ctcccggatg agcttgaaga
     3061 ggtcgagcgg ccgttccggc cccagcgcgt agagcgtccg gggtcggtgc agttcgtcat
     3121 tgaggtcggc gtagcgcccg ccgacgcgat cgagggcgta gagggtgtgg agccccagca
     3181 ggtcggccca gtaatgccac ttgaggacgc gggcgtcgac ggagagttgg tcgccgtcga
     3241 tgtcgtaggt agcgctccgg ccgtcggcga aggtgaacgc cgcgctgaat cggtgcgggc
     3301 cctccggcgt gacgcgcacg gtggccgcca gctcctcgtc ggtgagcgcc cggtatcccc
     3361 gggtgccgac ggccacgagc gcggcgatgg ccgcgagcga cacgaagagc gccgcgagga
     3421 gggcgcgtgt gagggtcgcg aacggccgcc gcttccggag tgcccggcgc gcaagcacga
     3481 ggcacaccag ggcaacggcg gcacaggcga tcccgacgac cgtgagggcg ctcaccgggc
     3541 ccggccgagc cccaggacca gggtcgcgat cccgtagacc agggccggcg ccaccgggag
     3601 cgcggggtcg gagtacgagg ccacctgcgc cgcgcggtcg gccgggacca tcttgagcac
     3661 gtgattcatc cccgggatca gcaggagccg ggcggcggga tccgcggcgg caaggaggcg
     3721 ggcatccagc gtgtcgacct ggatgtcggt ggtaccctgg gcgatcagca ccgggacctt
     3781 gagcgccgcc aagtcccgcg ccggatcgtg ccgcatccag gagatgagat acccctggac
     3841 gctctgccga tagagcatcc agagcctcgg cggcacgctg tccacgcgct cgccggtgcc
     3901 gaggcgggcc atgatcgagt ccgcctggcg gaggagatcg ggcgggagct ggggggcgag
     3961 ctgttcgtgg aggatctgcg cgatgggccg ccccgcccct gcgatcgaca cgaaggcgtc
     4021 ggcgccggcc tcggcggccg cgagcattcc cacgagcgat ccttcgctgt gccccacgac
     4081 cgtgaccgag cggaagcggc ggtcggcctt gagcgcgcgg atccacgtgg ccgcgtcgcc
     4141 gacgtagtcg tcgaagcgga ggtcggcctc gcgaagggcg aatccgttgc tcgcggcgat
     4201 gccgcgcttg tcatagcgta ccgacgcgat gccgcgggcg gccagggcgt cagccaggta
     4261 ccgaaggctg ttgttccggc cggccaggag gggggagttg ccgtcgcggt cggtggggcc
     4321 cgagcccgag atgaagagta ccgccggcca ggggcccggc gtcgtgggcg tcgcgagcgt
     4381 tccccggagg atcccacccg gcactgcgag cgaatcggcg aacgacgtgt ccgtcggcgc
     4441 ctgggccacg agggccgccg ggagcagcag cgccgcgagc gccacggccc gcctcatctg
     4501 ggcaccccgc cccggagcac cgcggccgag tcggtgagcc gcaccccggc ggcgcgccac
     4561 tcggcgagca gcaggtcggt gcgctcggga tggatggcgc gggcggcgtc ggtcacgaac
     4621 cagagctcga tggccgggta tcgccgccgg agcccctcga tcgcgtactt gtcgcagaca
     4681 tcggtcgcga caccgtagag cacgatgcgg tcgggcgcga gcgcgtcgac caccgccggc
     4741 acgttctcgt tggtgaatac gtcgaaccag tgcttgtgga gcaggatgtc gccgcgatgg
     4801 gcccgcaccc gcgccgcgac gcccgggtcg acctcgggtt cgatgaccag cgggtcacgg
     4861 agcgccgtct cggcgacctt ccgctgaccg ggggtccccc gcatgcagtg gtcggggaac
     4921 gtcgtcagga agtccggcgc ggtggagagc tcgcggtggc cggggacgtg gtcgtcggcg
     4981 ctggcgacga tccggatgcc ttcccggtgc gcgtagtcgg tcagggcccg gagcaggggc
     5041 gcgatcgcct cggcacccgg gacataaagc ttcccgtccg gtcgcatgaa gtcatgctgg
     5101 gtgtcgacgt cccagaagat ggtcgcggcc atggctgata ataagcgacg tgagcggtga
     5161 gcggtaagcg gtgagagggc gcccagagcg cggcgccagg aatggcctga acgatccccc
     5221 cagcgggcgg atcttccgga cgccatcccc gcctcccgcc ccacctctca cctcttcccg
     5281 ctcactccac cctctccagc ctcgggttcg gccgatactc ccgcccgacc ttggcacagg
     5341 gcttcccttc ccgcagcacc acgtcggcgg tgaagtcgta tcgcccctgg aagagggagc
     5401 tgccgacgcc gtaggcgtct acgggcacct gctgcgcctc gaactcgcgg atcttctcga
     5461 ccgtgaagcc ccccgacacg atgatgcgca cgccctggaa cccctcgcgg tcgagcgcct
     5521 cgcggacgtt gcggacaagc tgggggttga ccccgttggg cttgaagcgc cccatcgagt
     5581 cccagagcga gcggtcgacg agcatttcgc tggtgtcgag gcggacgccg tagagccggg
     5641 agccgagggc gcgggcgcat tcgaccgagg tgcggacgca gtcattctcg aagtccgcga
     5701 gcgtcaccac ctggacgctc gacgggatgt actcggcgaa tttccgggtg gcgagcaccg
     5761 tatcgccccc gtaggcggcg atgagggcgt gcggcaccgt gcccacgccc tcggagcccc
     5821 accaggaggc ctgggcgtcg gtcgagacgc cgatggcccc cgcgacgtgg gcagcgtagc
     5881 cgtcgccggt ctgcaccagc cagtggtcgt gccgggccgg gaagaacatc acttccttgg
     5941 ggtgcgccgc ttcgaccacc cgccgggtgt tggtgccgac ccgggtgcgg cgggcaagga
     6001 cgcccagata gagcgtctcg aggtgggcaa aggcgtcgta ggggccttcg atgagcatca
     6061 ccgtctccca cggttcgacc tcgtccccct cgtggagggc gtggacggtg agcgcaggcc
     6121 actcgacggc gcagagcttg aggatggcca cggcctcgtc gatgccaccc aggaaggcgt
     6181 gcgacttgcc gaagacctgc atcgtcacgc gcggatgggt gccgtcggcc aggaggatct
     6241 cccgggcgcg gacgaagtat ttatcggagt agtagcccgc gcgcatcttc tcgacgggaa
     6301 gattgaagat cgcgggatcg agcctcgggc ggcgcgcgag cattcaggga gcctggttct
     6361 ccgccggttc ggcgggcagg aatcgggacg agatgtcggc gagggcgccg cggatccggg
     6421 tgacctgcgg cgggtcaggg tggcggaaga cgagccggcg gaattcgcgc agcgcggccg
     6481 ccgggtcgtg cagtgcttcc tcgtggagcc gcaccaggag gaggcccacc cgcacgtcat
     6541 cgtcggcgtt gaggcgatgg gcggcccgca gcgcctcgag cgccccgacc gccgcggccg
     6601 ccccaccggg aggaccggcc cccgcttcgg cctgctggat cgcggcatcg acgtcggccg
     6661 aggggagggc gggcgcacga tagcgcgcct ccgcggcgcg gaactgctcg gtcgccgtga
     6721 gcgcaccgac ctggctgccg gatccggggg catcgtggcg caggtcgtcg ccgatggccg
     6781 cgggcaattc ggcgaccggc gcgaggaatc cgttcacgaa ggcgcgcatc accccgtaga
     6841 tggcccaggt cgccgtggcc agctgaagca tccgcggctc gcccagcgcc agccagacga
     6901 tgagcgacac gagggtggcg cccaccagca ggatgcgcag caccgcgcgc aggacgtcga
     6961 gcgggtcact accgtcgagc agggacatcg cggcccggtt gaggagaacg ggaaaggtac
     7021 ggcccggcgt gccggcggag caagttgaca ggccgacccg acccgcgtaa atcccggtgc
     7081 cccttgatgt tccttccccg aatggagcct tgcccgtgcc tgattccgtc tcccgccggg
     7141 aattcctcgc cgcgaccggc gccgtggtgg ccgcttccgg cgccacccac cccgttcccg
     7201 ggttcccggc ggtgcatatc cgcccccccg ccaaggccgt cgccgtgagc tcggccaacg
     7261 ggttccgcgg cgtccagaag gcggtagacc tgatgatcac ggggaccgac acgctggatg
     7321 cggccatcga gggggtcaag atccaggagc tcgatcccaa tgaccactcg gtgggctacg
     7381 gcggtctccc caacgaggac ggcgtcgtgc agctcgatgc ctcgtgcatg cacgggccga
     7441 cccggcgggc cggggccgtc ggtgcgctcg aggggatcaa gctcgccagc gagatcgcgc
     7501 gcctggtgct caagtacact gatcacatca tgctggtggg tgagggtgcg aagaagttcg
     7561 cgctgtcgta cggctaccag gaagaggacc tgctgacgcc cgaagcgcgg cagatctggc
     7621 tcacctggcg cgccaaccgc ggcaacaagg acgactggat gaacgtgccg gacgggacgc
     7681 cgatggtgca ccgccccacc ggcacgatca acatggatgt ggtcaacgcc ctgggggaga
     7741 tctcctcggt caccacgacg gcggggctcg cgttcaagat ccccggccgg gtgggcgatt
     7801 cgccgatcat cggggcgggc cagtacaccg acaacgacgt cggtgcggcg ggctcgaccg
     7861 gcctgggcga gctcaacatc gtcgcgtgcg gggccttctt caccgtggac ggcatgcggc
     7921 ggggcctcaa gccgacggac gcggcgatcg agacgctcaa gcgggtcatg caactggccc
     7981 cgccgagcct ggtggcggcc aacgggaagc cgaagttcca gctcaactac tacgcggtca
     8041 acaagaaggg ggagtacggc tccgccgccc tctatcccag caag
//
LOCUS       DASUAP010000006         9215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1117615, whole genome shotgun sequence.
ACCESSION   DASUAP010000006 DASUAP010000000
VERSION     DASUAP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117615"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..867)
                     /locus_tag="VFU45_00220"
     CDS             complement(85..867)
                     /locus_tag="VFU45_00220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU5357511.1"
                     /translation="MLSYQHLVDTARAAADIAARYLRDTRPPEPSAWEVKSPRDFVTS
                     VDRAAEEMIREALLRTEPGSRVLGEEFSPDERATGLLWIVDPLDGTTNFLHGVPAWAV
                     SIGVAVDGELVGGVVHDVPHGVVSHASLGAGAWQGARRLSVSGTRDPAQALIGTGFPF
                     KDLADADRYLAQFRRVMAATSGIRRPGAASLDLVSVASGAFDGFWELSLAPWDVAAGT
                     VIIREAGGRLTGFDGTDRGIGHGPVVAGNPWIHEWLLTTLRD"
     gene            904..1911
                     /gene="trpS"
                     /locus_tag="VFU45_00225"
     CDS             904..1911
                     /gene="trpS"
                     /locus_tag="VFU45_00225"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU5357512.1"
                     /translation="MPRVFSGIQPSGELHIGNWLGAIKNWVELQQRYDCMFSVVDLHA
                     ITGKYEAATLAGRTREMALGILAAGVDPAKAVLFVQSHVPAHSELQWLLTTLTPLGEL
                     ERMTQFKDKARRVESVPAGLLAYPVLMAADVLLYRADLVPVGDDQVQHLELAREICRK
                     WNAAFGGGADFLPEPQPILGGGRRILGLDGQAKMSKSLGNTVGLLDPPDEIWKKVRMA
                     VTDPARITRHHPGNPDICNVYALHKHFSPPETVVEVADNCRGAKWGCVDCKRVLAENI
                     GRTLAPMRERAAELAADPGAVDRTLAAGAEVARERAAGTLREVKARMGFLPPATAPRG
                     T"
     gene            1908..3011
                     /locus_tag="VFU45_00230"
     CDS             1908..3011
                     /locus_tag="VFU45_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEU5357513.1"
                     /translation="MTDPTQAQGAGTGIEKIIKAAVERGASDLHIKAGDVVRARVHGT
                     LVPLTKQRLTPAQTRAIALKLLPGDAERNALDELRDYDCSWGMPGVGRFRVNILRQRS
                     SFMVVMRVIPFALPTFEGLGLPDVVRQLSESDRGLVFVTGVTGSGKTSTVAAMVHHVN
                     QTLQKHVVTLENPIEYLHRDINCSVTQREIGVDTESFAVGLRAALRQDPDLLVIGDIR
                     DTEIMDLALKGAEEGHLVVSVMPTPDVTSTVKRLLSLFPPEEREIGRVRLSEALRAVV
                     AQRLLPKKDGSGRVAAVEVMVCTPRIREVLRDPVRIDDLKVEMIRGRDEHGMQTFGQH
                     LDDLVERELVAIETARAALTEPGSLPVGAGAPA"
     gene            3097..4377
                     /locus_tag="VFU45_00235"
     CDS             3097..4377
                     /locus_tag="VFU45_00235"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU5357514.1"
                     /translation="MFDPKHHCVVVVGAQWGDEGKGKLVDVLAERADVVVRYQGGANA
                     GHTVVIGEEQFILQQIPSGVLHAGVTCVVGNGVVLDPEQFFAELDGLTAKGVDIAGRV
                     RVSERAHLVLPYHKLLDQANESSQKIGTTGRGIGPAYEDKFGRRGVRVGDLADLPTVS
                     TLVRERVERANRVLAMLGSSERASADEHVALLERLAPRLVPMSVDAGLLVHRALRGGK
                     RVLLEGAQGALLDVDHGTYPFVTSSNTTAGGAATGAGIGPTAIDGVLGVVKAYTTRVG
                     NGPLPTEALGAEGEELRKIGAEFGAVTGRPRRCGWFDATVVRYSVRVNGLTGLAVTKL
                     DVLDTFAQIPVGTAYTLDGASCEEMPSDIERLGRIKPVYETLPGWQRSTAEARRLADL
                     PPQARAYLDRLQDLAGAPIRYVSVGTRRDQIIEV"
     gene            4571..5899
                     /locus_tag="VFU45_00240"
     CDS             4571..5899
                     /locus_tag="VFU45_00240"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEU5357515.1"
                     /translation="MADATLMDKLVSLCKRRGFVFQSSEIYGGLGSVWDYGPLGVELK
                     KNVKDRWWRAMVHERDDIEGLDAAILMHPKVWEASGHVAGFSDPLVDCRTCKARFRAD
                     KLEDARCPQKPSKQPGQHSTCDLTEPRNFNLMFRTFMGPVEDSAAVVYLRPETAQGIY
                     VNYLNVLNSSRQKVPFGIAQIGKAFRNEITPGNFIFRTREFEQMEMQFFVTPGSDLEW
                     FERWREWRMGWHHAIGLTPARLRWHQHGPGELAHYAKAAYDIEYEFPFGWSEIEGIHD
                     RTDFDLARHQEFSGKKLEYFDQAANTRYLPYVVETSAGADRVTLTTLVDAYREEEVEG
                     ETRVVLGLHSAIAPIKAAVLPLVKKDGMPETATALYDDLRKRVRCFYDDSGAIGRRYR
                     RMDEVGTPFCITVDGQTSQDQAVTIRHRDAMTQERIGIDQVAGWLGERLG"
     gene            6096..6365
                     /locus_tag="VFU45_00245"
     CDS             6096..6365
                     /locus_tag="VFU45_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357516.1"
                     /translation="MTHECTTTLPGAEVLARAVTFFAERVPQYAAFLDERGPAFVTLR
                     GQGGEEVAIAVSAAGAATHVRASTLFFDQAVARFLNTLPAAEVVA"
     gene            6362..6622
                     /locus_tag="VFU45_00250"
     CDS             6362..6622
                     /locus_tag="VFU45_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357517.1"
                     /translation="MSTMVPVRVRVHDSWEEVALELDARAPVAELKQRALTGARRPGD
                     PARYEVKFRGAAILDESASLESVGVVRNAELIVLPRRRQPVR"
     gene            6619..6873
                     /locus_tag="VFU45_00255"
     CDS             6619..6873
                     /locus_tag="VFU45_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357518.1"
                     /translation="MSGRFRTLTLAAVGFLGLDGVLFLWLAGAAHRPGFYWAAAGCWL
                     VAGLVVLAAREQRQRLEAIAREREAIRRAAEELRELAHRR"
     gene            6870..7763
                     /locus_tag="VFU45_00260"
     CDS             6870..7763
                     /locus_tag="VFU45_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEU5357519.1"
                     /translation="MTVIFTHPDCLLHDPGQGHPEVPERLRTVLDRARSAKHEVREAA
                     AAAIDALQDIHPDAYVRDIESMCLRGGAIIGQDTVLNRSSYAAALGGLGAILAAVEHA
                     HAGRGNAFAAVRPPGHHALRDRAMGFCIFANAVVAARHAQRLGRARVLIVDWDVHHGN
                     GTQALVEHDATISYVSMHQWPWYPGTGAAAERGVGNVFNVPQGPGLPAARYVEDLWAA
                     VEAAARFAPPDAVILSAGYDSMRGDPLGGFTLEPADYATWTARIRERFPRAPLIGVLE
                     GGYAPARLADGVAATVGAMDA"
     gene            7887..8909
                     /locus_tag="VFU45_00265"
     CDS             7887..8909
                     /locus_tag="VFU45_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEU5357520.1"
                     /translation="MPKLHLEAEVQTLRTRHPFIIARGGKSEYRTVVVKLRDADGLEG
                     WGEAAGIAYYGETPETALAALQAYAQVLPASAFDLESAERGFEAALKGNPGARAALSA
                     ALHDLVGKRLGVPLWKLWGLDPARAPKSTFTIGLDTVEQMQAKVREAAEYPILKVKLG
                     TDRDVDILRGLREVTDKELRVDANCGWTVKRAIQMLPVLEEFGVTVLEQPVEPGDLDG
                     LGEVRRHSSLPVIADESCLVAAHIPALVGRVDGINIKLAKCGSLREAIRMVHVARAHG
                     LAVMVGCMIESSLAITAAAHFTPLVDIVDLDGAALLKDDPFVGATIANGQVRLPDAPG
                     LGVTRR"
     gene            8906..>9215
                     /locus_tag="VFU45_00270"
     CDS             8906..>9215
                     /locus_tag="VFU45_00270"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357521.1"
                     /translation="MTDGAGFAQVALPLAVPEPYTYQVPDALLDRVAPGARVVVPLRN
                     REVVGVVTATDAPPPPAAAKPILGVLDPEPALPRPLLETVQWMAGYYGAPLGLALKAV
                     L"
BASE COUNT         1391 a         3143 c         3378 g         1303 t
ORIGIN      
        1 caaggcccgc cacgaccttg gcgccctggg ccgggagggg gagcgcggca cccagcgcca
       61 gggcggcgcc gagggcgagg ccggtcagtc gcggagggtc gtgaggagcc attcgtggat
      121 ccacgggttg ccggccacca ccgggccgtg gccgatgccg cgatcggtcc cgtcgaaccc
      181 ggtgagccgg ccgcccgcct cgcggatgat gacggtgccg gccgcgacgt cccacggagc
      241 gagcgaaagt tcccagaagc cgtcgaacgc gccgctggcg acgctcacca ggtcgagcga
      301 tgcggccccg gggcgacgga tgccgctggt ggcggccatc acccggcgga actgggccag
      361 gtaccggtcg gcatcggcca agtccttgaa ggggaacccg gtcccgatga gcgcctgggc
      421 ggggtcgcgg gtgccggaga cgctgagccg gcgggcaccc tgccaggcgc ccgcaccgag
      481 gctcgcgtgg gagacgacgc cgtgcggcac gtcgtgcacc acgccgccga ccagctcgcc
      541 gtccaccgcg acgccgatcg acaccgccca cgcggggacg ccgtgcagga agttggtggt
      601 gccgtcgagg gggtcgacga tccagaggag accggtcgcg cgctcgtcgg gggagaactc
      661 ctcgcccagg acgcggctgc cgggttcggt cctcaagagc gcctcgcgga tcatctcctc
      721 cgccgcgcgg tccaccgatg tgacgaagtc gcggggcgac ttgacctccc acgccgaggg
      781 ctcgggcgga cgggtgtccc gaagatagcg ggcggcgatg tcggccgcgg cccgggcggt
      841 gtccactaag tgctgatagg ataacatctt gcggctccgg cccaggcacg gtatgttgcg
      901 ccgatgcctc gcgtcttctc agggatccag ccctcgggcg aactccacat cggcaactgg
      961 ctcggcgcca tcaagaactg ggtcgagctg cagcagcgct acgactgcat gttctccgtg
     1021 gtggacctgc acgctatcac cggcaagtac gaggcggcaa ccctcgcggg ccgcacccgg
     1081 gaaatggcgc tcgggatcct ggccgccggc gtcgaccccg cgaaggccgt cctcttcgtg
     1141 caatcgcacg tgccggcgca cagcgagctc cagtggctcc tcacgacgct cacgcccctg
     1201 ggcgaactgg agcggatgac gcagttcaag gacaaggcgc gccgggtcga gagcgtaccg
     1261 gccgggttgc tggcgtaccc ggtgctgatg gcggccgacg tgctgctcta ccgcgccgac
     1321 ctggtgccgg tgggggacga ccaggtgcag cacttggagc tggcgcgcga gatctgccgg
     1381 aagtggaatg ccgccttcgg cggcggcgcc gacttcttgc cggagccgca gccgatcctt
     1441 ggcggcggcc gacggatcct cgggctcgac ggccaggcca agatgtcgaa gagcctgggc
     1501 aacacggtcg ggttgctcga cccgccggac gagatctgga agaaggtccg catggccgtg
     1561 acggatccgg cccggatcac gaggcaccac ccgggcaacc ccgacatctg caacgtgtac
     1621 gcgctgcaca agcatttttc cccgcccgag acggtggtgg aggtggcgga caactgtcgc
     1681 ggtgccaagt ggggctgcgt cgactgcaag agggtgctgg cggagaacat cggccggacg
     1741 ctggcgccga tgcgggagcg ggccgccgag ctcgcggccg atcccggcgc ggtcgaccga
     1801 acgctggcgg cgggggcgga ggtggcgcgg gagcgggcgg ccggcacgct ccgggaagtg
     1861 aaggctcgga tgggattcct gccgccggcc acggcgccga ggggcacatg acggatccga
     1921 cgcaggcgca gggtgcgggc accgggatcg agaagatcat caaggctgcc gtggagcggg
     1981 gggcctccga cctccacatc aaggccggcg acgtcgtgcg cgcgcgcgtg cacgggacgc
     2041 tggtgccgct caccaagcag cgcctgacgc cggcgcagac gcgggccatc gccctcaagc
     2101 tcctgccggg cgatgccgag cggaacgcgc tcgacgagct ccgggactac gactgttcgt
     2161 gggggatgcc cggtgtgggc cgcttccggg tcaacatcct gcggcagcgc tcctccttca
     2221 tggtggtcat gcgggtgatc ccgttcgccc tgccgacgtt cgaggggctc gggcttcccg
     2281 acgtggtgcg tcagctctcg gaatccgacc ggggtctcgt cttcgtcacg ggtgtcacgg
     2341 ggagcggcaa gacgtcgacc gtggcggcga tggtgcacca cgtcaaccag acgctgcaga
     2401 agcacgtcgt gacgctcgag aacccgatcg agtacctcca ccgggacatc aactgctcgg
     2461 tgacgcagcg cgagatcggg gtcgacaccg agagctttgc cgtggggctc agggccgcgc
     2521 tccggcagga tccggacctc ctcgtgatcg gcgatatccg ggacaccgag atcatggacc
     2581 tggcgctcaa gggggcggag gaagggcacc tggtggtgtc ggtcatgccg acgccggacg
     2641 tgacctcgac ggtcaagcgg ctgctctcgc tcttcccgcc ggaggagcgc gagatcggcc
     2701 gggtgcggct ctcggaggcg ctccgggcgg tggtggcgca gcggctcctg ccgaagaagg
     2761 acggcagcgg ccgggtggcg gcggtggagg tgatggtctg cacgccgagg atccgggagg
     2821 tcctgcgtga cccggtgcgg atcgacgacc tcaaggtcga gatgatccgg ggccgcgatg
     2881 agcacgggat gcagaccttc ggccagcacc tggacgacct ggttgagcgc gagctggtgg
     2941 ccatcgagac cgcccgcgcg gcgctcaccg aacccgggtc gctgccggtc ggggcggggg
     3001 cgccggcctg attgaccgga tgcggggccc cgcgtagctt tcacccaagg atccacgggc
     3061 cccgtcgggg cccttttttg ttggaggcgg gccaggatgt tcgatccgaa gcaccattgc
     3121 gtggtggtcg tcggcgccca gtggggcgac gagggcaagg gcaagctcgt cgacgtgctg
     3181 gccgagcggg ccgacgtcgt cgtccgctac cagggcgggg ccaacgcggg gcacaccgtc
     3241 gtgatcgggg aggagcagtt catcctccag cagatcccgt ccggtgtcct gcacgcgggg
     3301 gtgacctgcg tcgtggggaa cggcgtggtg ctcgacccgg agcaattctt tgccgagctg
     3361 gacgggctca ccgccaaggg cgtcgacatc gcggggcggg tgcgggtgtc ggagcgggcc
     3421 cacctggtgc tgccgtacca caagctgctc gaccaggcca acgagtcgag ccagaagatc
     3481 gggaccacgg ggcgggggat cggcccggcc tacgaggaca agttcgggcg ccgcggcgtg
     3541 cgggtgggcg acctggccga cctcccgacc gtgtcgaccc tcgtgcggga gcgggtcgag
     3601 cgggccaatc gggtcctcgc gatgctgggc tcgtccgagc gcgcctcggc cgacgagcac
     3661 gtggcgctgc tggagcggct ggcgccacgc ctcgtgccga tgtcggtgga tgcgggcctt
     3721 ctggtgcatc gggcgctccg gggcgggaag cgcgtcctcc tcgaaggggc gcagggcgcg
     3781 ctgctcgatg tggaccacgg cacctatccc ttcgtgacga gcagcaacac caccgccggc
     3841 ggcgccgcga cgggggccgg catcgggccc accgccatcg acggcgtgct cggggtggtc
     3901 aaggcgtaca ccacccgcgt gggcaacggt ccgctcccga ccgaggcact gggagctgag
     3961 ggagaggagc tccggaagat cggcgccgag ttcggcgcgg tgaccggccg gccccggcgc
     4021 tgcgggtggt tcgacgccac cgtggtgcgc tattcggtgc gggtgaacgg gctcaccggg
     4081 cttgcggtca cgaagctcga cgtgctggat accttcgccc agatcccggt gggcaccgcg
     4141 tacacgctcg acggcgcgag ctgcgaggag atgccgtccg acatcgagcg tctgggccgg
     4201 atcaagccgg tctacgagac gctgccgggg tggcagcgct cgaccgccga ggcgcggcgc
     4261 ctggccgacc tgccgccgca ggcccgggcc tacctcgacc ggctgcagga cctggcgggc
     4321 gcgccgatcc ggtatgtgag cgtggggacg cggcgggacc agatcatcga ggtgtgaggt
     4381 ggtgaacggg caacggtgaa cggtgaacgg gggcggacaa gatccaagtc ggagcgcccg
     4441 ttcaccgctc accgctcacc gcttaccgct taccgttcac cgactttcgc ccgccggccg
     4501 tctcccctac ctttaatgcc agccagtcgc tcaaccgggg cggcccttcc ttttccttcc
     4561 tgaccgatcc atggccgacg ccaccctgat ggacaagctc gtgtcgctct gcaagcggcg
     4621 cggtttcgtg ttccagtcct ccgagatcta cggcgggctc ggctcggtgt gggattacgg
     4681 ccccctcggc gtggagctca agaagaacgt gaaggatcgg tggtggcggg caatggtcca
     4741 cgagcgcgat gacatcgagg ggctcgacgc cgccatcctg atgcacccga aggtgtggga
     4801 ggcgtcgggg cacgtggcgg ggttcagcga tccgctggtg gactgccgca cctgcaaggc
     4861 gcgcttccgg gccgacaagc tcgaggacgc gcgctgtccc cagaagccga gcaagcagcc
     4921 gggtcagcac tcgacgtgcg acctcacgga gccgcggaac ttcaacctca tgttcaggac
     4981 gttcatgggc ccggtggagg actccgcggc ggtcgtgtac ctgcggccgg agacggcgca
     5041 gggcatctac gtcaactacc tcaacgtcct caacagctcc cggcagaagg tgccgttcgg
     5101 gatcgcgcag atcgggaagg cattccgcaa cgagattacc ccggggaact tcatcttccg
     5161 cacccgcgag ttcgagcaga tggagatgca gttcttcgtg accccggggt ccgacctcga
     5221 gtggttcgag cggtggcgcg agtggcggat ggggtggcac cacgcgatcg ggctcacccc
     5281 cgccaggctc cgctggcacc agcacggccc cggcgagctg gcgcactacg ccaaggcggc
     5341 gtacgatatc gagtacgagt tccccttcgg ctggagcgag atcgagggga tccacgaccg
     5401 caccgacttc gacctggccc gacaccagga gttcagcggg aagaagctcg aatacttcga
     5461 ccaggccgcg aacacgcgct acctcccgta cgtcgtcgag acatcggcgg gcgccgaccg
     5521 ggtgacgctc accacgctgg tcgatgccta ccgggaggag gaggtcgagg gggagacgcg
     5581 ggtggtcctc ggcctccact cggccatcgc gccgatcaag gccgcggtgc tgccgctggt
     5641 caagaaggac gggatgcccg agaccgccac cgcgctctac gacgacctcc gcaagcgcgt
     5701 ccggtgcttc tacgacgaca gcggcgccat cggccggcgg taccggcgga tggacgaggt
     5761 cgggacgccg ttctgcatca ccgtggacgg ccagacgtcg caggaccagg cggtgaccat
     5821 ccgccaccgc gacgcgatga cccaggaacg gatcgggatc gaccaggtgg cggggtggct
     5881 gggggagagg ctaggctaga gggaagaggg aaaagtgaaa agggaaaagg gaaaagggaa
     5941 gagtgaaaag ggaaaaggga gagggaatag ggtcgacgcg atcttatcgg tccacgacct
     6001 gtgacccgcg attcacgact cacgaccctc gactcacgac tcacgacccg cgactcacga
     6061 ctaacgacca ctgaccaacg accaacgact ctcctatgac ccacgaatgc accaccaccc
     6121 tgccgggcgc cgaggtactg gcgcgggccg tcacgttctt cgccgagcgg gtgccgcagt
     6181 atgccgcgtt cctggatgag cgtgggcccg cgttcgtgac gctgcggggc cagggtggtg
     6241 aagaggtggc gatcgccgtg tcggccgccg gcgccgccac ccacgtccgc gcgtcgacgc
     6301 tcttcttcga ccaggcggtg gcccgattcc tcaacaccct gccggcggcg gaggtggtgg
     6361 catgagcacg atggtgccgg tccgggtgcg ggtgcatgac tcgtgggaag aggtggccct
     6421 ggagctcgat gcgcgggcgc ccgtggccga gctcaagcag cgcgccctga ccggggcccg
     6481 gcgccccggc gatccggcgc gctacgaggt caagttccgg ggcgccgcga tcctcgatga
     6541 gtcggcctcc ctcgagagcg tgggcgtggt ccggaacgcc gagctgatcg tcctgccgcg
     6601 gcggcggcag ccggtgcggt gagcgggcgg ttccggacgc tgaccctcgc cgccgtcggg
     6661 ttcctcggac tcgacggcgt gctctttctc tggctcgccg gcgcggcgca ccgccccggg
     6721 ttctactggg ccgcggccgg atgctggctg gtggccgggc tcgtggtgct cgcggcgcgg
     6781 gagcagcgcc agcggctcga ggcgatcgcg cgggagcgcg aggcgatcag gcgggcggcg
     6841 gaggaacttc gggaactggc ccaccggcga tgaccgtcat ctttacccac cccgactgcc
     6901 tcctgcacga tccggggcaa ggacacccgg aggtcccaga gcggctgcgc acggtgctgg
     6961 accgcgcccg cagtgcaaag cacgaggtgc gggaggccgc cgccgcagcc atcgacgcgc
     7021 tccaggacat ccaccccgac gcctacgtcc gcgacatcga gtcgatgtgc ctccgcggcg
     7081 gtgccatcat cggccaggac acggtcctca accgctccag ctacgccgcc gccctcggcg
     7141 gactcggcgc catcctggcc gcggtggaac acgcccacgc gggccggggt aatgcctttg
     7201 ccgcggtgcg gccccccggc caccacgccc tgcgggaccg ggcgatgggc ttctgcatct
     7261 ttgccaacgc cgtggtcgcc gcgcgtcacg cgcagcgcct cggccgcgcc cgggtgctca
     7321 tcgtcgactg ggacgtccac cacgggaacg ggacccaggc gctggtcgag catgatgcga
     7381 cgatctccta cgtctcgatg caccagtggc cctggtatcc cggcaccggc gccgcggccg
     7441 aacgtggcgt ggggaacgtc ttcaacgtgc cgcagggccc cggactcccg gccgcgcggt
     7501 atgtggagga cctctgggcc gcggtcgagg cggcagcaag gttcgccccg cccgacgcag
     7561 tcatcctgtc ggcgggctac gattcgatgc ggggcgaccc gctcggcggc ttcaccctcg
     7621 aacctgccga ttacgccacg tggacggcac ggatccggga gcgcttcccc cgcgccccgc
     7681 tcatcggcgt cctggaaggg ggctatgcgc cggcccggct ggccgacggg gtcgcggcga
     7741 cggtcggggc gatggatgcc tgaccttgcg gtggcgcccg gatcggcggg cgcagcatgc
     7801 tccgcccctg cctgccggat gacttcgctg cgtccggccg gtatcttctg acgtctcacg
     7861 tctcacgtct tatgtctcac gaccctatgc ccaaactcca tctcgaagcc gaagtccaga
     7921 ccctccggac ccggcacccg ttcatcatcg cccgcggcgg gaagagcgag taccgcacgg
     7981 tcgtggtcaa gctgcgcgat gccgatgggc tcgaggggtg gggcgaagcg gcgggcatcg
     8041 cctactacgg cgagaccccg gagacggcgc tggccgcgct gcaggcatac gctcaggtgc
     8101 tccccgcgtc ggccttcgac ctcgaatcgg ccgaacgagg cttcgaggcc gccctcaagg
     8161 ggaaccccgg tgcccgtgct gcgctctcgg cggcgctcca cgacctcgtg ggcaagcgcc
     8221 tcggcgtgcc gctctggaag ctctggggcc tcgaccccgc ccgggcgccg aagagcacct
     8281 tcaccatcgg cctcgacacg gtcgagcaga tgcaggccaa ggtgcgggag gccgccgagt
     8341 atccgatcct caaggtcaag ctcggcaccg accgcgatgt cgacatcctg cgcgggctcc
     8401 gcgaggtgac cgacaaggag ctccgggtcg acgccaactg cggctggacc gtgaagcgcg
     8461 ccatccagat gctgccggtg ctcgaggagt tcggcgtcac cgtgctcgag cagccggtcg
     8521 agcccggcga cctcgacggc ctgggcgagg tgcgccgtca ttcctcgctc ccggtcatcg
     8581 ccgacgagag ctgcctcgtg gcggcgcaca tcccggcgct ggtgggcagg gtcgacggga
     8641 tcaacatcaa gctcgccaag tgcggctcgt tgcgggaggc gatccggatg gtccacgtgg
     8701 cgcgcgcaca cggcttggcc gtgatggtcg gctgcatgat cgagagctcg ctcgcgatca
     8761 cggccgcggc gcacttcacc ccgctcgtcg acatcgtcga cctcgacggc gcggcgctcc
     8821 tcaaggacga cccgttcgtc ggcgccacga tcgcgaacgg gcaggtccgc ctccccgacg
     8881 cccccgggct cggcgtcacc cgccgatgac cgacggcgcc gggttcgccc aggtcgccct
     8941 cccgctcgcg gtcccggaac cctacaccta ccaggtcccc gacgccctcc tcgaccgggt
     9001 ggcccctggc gcgcgcgtgg tggtgccgct ccggaaccgg gaggtcgtcg gcgtggtgac
     9061 ggccaccgat gcgccgcccc cgcccgccgc ggcgaagccg atcctgggcg tcctcgatcc
     9121 cgagccggca ctgccgcgcc ccctgctcga gacggtgcag tggatggcgg ggtattacgg
     9181 cgcgccgctc ggcctggcgc tcaaggcggt gctgc
//
LOCUS       DASUAP010000007         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_245509, whole genome shotgun sequence.
ACCESSION   DASUAP010000007 DASUAP010000000
VERSION     DASUAP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245509"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFU45_00275"
     CDS             complement(<1..184)
                     /locus_tag="VFU45_00275"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HEU5357522.1"
                     /translation="MSMTETAVLAGGCFWCLEAVFERLRGVEQVVSGYTGGTRPDPTY
                     EQVCSGATGHAEAIRVT"
     gene            complement(229..807)
                     /locus_tag="VFU45_00280"
     CDS             complement(229..807)
                     /locus_tag="VFU45_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEU5357523.1"
                     /translation="MSAGTPGGRRRAVFLDRDGTLIEDTGYTARPEEVRLIDGAAAAV
                     ARINAAGYLAIVATNQSGIARGLITLEQYHAVAARTAELLAAQGARLDAQFFCPHYPD
                     VTGPCDCRKPGVKLYRDADAQFGIDFARSVWIGDRLRDVEPARTLGGTGILVLTGQGA
                     GDADAARTEGFRVVADVGTAVRRVVQAGNRRS"
     gene            complement(804..2165)
                     /gene="selA"
                     /locus_tag="VFU45_00285"
     CDS             complement(804..2165)
                     /gene="selA"
                     /locus_tag="VFU45_00285"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEU5357524.1"
                     /translation="MTDPRRALPSVDRLLKEPEVSALLDTVPRSAVVAAVREVLEGAR
                     KRAGVPDDWALAIRERLVEREAPSLVPVLNATGVVLHTNLGRAPLAESAQAAIAAVAG
                     GYSTLEFDLPTGIRGHRGDHSRGLLAELSGAEDGLAVNNAAGALVLALQALARGREVV
                     ISRGELIEIGGSFRIPEILETSGARLREVGTTNRTHLRDYEAAIGADTGAVLIVHRSN
                     FAQRGFVTSPAPSEVASLAAARGVPCIYDVGSGLLADLTPWGLVSEPRVPDAVASGAD
                     LVVYSGDKLLGGPQAGCLVGTAEMVRRCREHPMARALRADKLTLAALDATLRLYRDPE
                     RARREIPTLAMLTTGPEELSARAARLRDLCPASLTPRTIPGSSSVGGGSFPDAELPTT
                     LVSLDPGPLGADGLALRLRLGRPAVVARVKDGQILLDPRTLPEAAFADVGAALRRALE
                     AET"
     gene            complement(2162..3580)
                     /locus_tag="VFU45_00290"
     CDS             complement(2162..3580)
                     /locus_tag="VFU45_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5357525.1"
                     /translation="MGGEVALCLLALLSACARIAPPPGGPPDLKPPVIVSTVPESTAV
                     LPDFKGDVEFIFDETVSEGTSADFGLGTGSLERLVLLSPTTKQPVVRWHRGMISVRPK
                     EGWKPGRVYRAELLPGVADLRRNVMKQGNVITFTTGAPLPDVTLRGTTIDWVGGRAAP
                     VAMVEAVLAPDSLVYRSASDSTGRFSFGPLPRGRYHIYAYIDQNHDLKRAYREPWDSV
                     SIVADRDSVNVPPFWMAPRDTTPPRFVEPQVTDSQTVTLGLNQPLDPYIDFDSVTVRL
                     LALPDSTPVTVASLLPKRIDDSLRARARAVADSIRADSIAKAHPDTGRKKAALPPAPP
                     AQPAPSLLAGLRGRPRVDSAMIKLIATRPPLTDRLVLRTATPLKVQGKYVIEVTGIRN
                     VNRVGGRIVGGIVVPKPPPPPKRPAIDSTKADSTKADSTRKLRPGAALPTPPDSTAER
                     LDSLKRHLRARPVPPPADSTKP"
     gene            complement(3667..>4336)
                     /locus_tag="VFU45_00295"
     CDS             complement(3667..>4336)
                     /locus_tag="VFU45_00295"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5357526.1"
                     /translation="GDQPGGDVARAVAYRVFDTALRLLHPVMPFITEELWQKLPGRAD
                     GEWLAAAAWPGSDRRAADPAALRQFALVQEAVGAIRQIRAEYGIEPGTQIAAVITPAS
                     PDAEAALAAEAGTVSRLAKLASLSLGRAEQGVGAHAVLSDGTPVFVPLGGAIDLAREC
                     GRLAEERTRLQGLVAAQERKLANGQFVARAPAAVVENERTKLSDWSRQVAVLEEKRKR
                     LGCG"
BASE COUNT          579 a         1553 c         1631 g          573 t
ORIGIN      
        1 acgtgacccg gatggcctcc gcgtgcccgg tggcgccgct gcacacctgc tcgtacgtcg
       61 ggtcggggcg ggtaccgccg gtgtagcccg aaacgacctg ctccaccccg cgcagccgct
      121 cgaagacggc ctcgaggcac cagaagcagc cgccggcgag cacggcggtc tcggtcatgg
      181 acattcacgc actccagtca gtgaacagac gctcggacgc tcaggcgctc aggagcgtcg
      241 attcccggcc tgcaccacgc gccgcactgc ggttcccaca tcggccacga ccctgaaccc
      301 ctcggtccgc gccgcgtcgg cgtcgcccgc tccctggccg gtcagcacca gtatcccggt
      361 cccgccgagc gtgcgggcag gctcgacgtc ccgcaggcgg tcgccgatcc agacgctcct
      421 cgcaaagtcg atcccgaact gcgcgtcggc gtcgcgatag agcttgaccc ccggcttccg
      481 gcagtcgcag ggaccggtca cgtcggggta gtgcgggcag aagaactgcg catcgagccg
      541 ggcaccctgg gccgccagca gctcggcggt ccgcgccgcc accgcgtggt actgctcgag
      601 cgtgatgagc ccgcgcgcga tccccgactg gttggtggcc acgatggcca ggtacccggc
      661 ggcgttgatc cgcgccaccg cagcggcggc gccgtcgatc aggcggacct cttccggccg
      721 cgcggtgtag ccggtgtcct cgatcagcgt cccgtcgcgg tcgaggaaca cggcgcgccg
      781 acggccgcca ggcgtcccgg cgctcacgtc tccgcctcga gggcccgtcg gagggccgcc
      841 cccacatcgg caaaggccgc ctccggcagg gtccgtggat cgagcagtat ctgtccgtcc
      901 ttgacccgcg ccaccaccgc cggccggccg agccggagcc ggagcgccag cccgtcggcg
      961 ccgagcgggc cagggtcgag ggagacgagc gtggtcggga gctccgcgtc ggggaagctc
     1021 ccgcctccga cggaggagga tcccgggatg gtccggggag tgagcgatgc ggggcagagg
     1081 tcgcggaggc gcgccgcccg ggcggagagc tcctccggtc cggtggtgag catcgccagc
     1141 gtcgggatct cgcgccgggc gcgttcgggg tcgcggtaca gccggagcgt cgcatccagg
     1201 gcggcgagcg tgagcttgtc ggcccggagt gcgcgggcca tcggatgctc gcggcagcgg
     1261 cgcaccatct cggccgtccc cacgaggcac ccggcctggg gcccgcccag gagcttgtcg
     1321 ccgctgtaga ccacgaggtc ggcgccggag gccaccgcgt cggggacgcg cggttcggag
     1381 acgagtcccc acggcgtgag gtcggccaag aggccgctcc cgacgtcgta gatgcacggg
     1441 acgccgcggg ccgcggccag cgaggccacc tccgatggcg ccggcgaggt cacgaacccg
     1501 cgttgcgcaa agttggaccg gtgcacgatg aggacggcgc cggtgtcggc gccgatcgcc
     1561 gcctcgtaat cgcgcaggtg ggtgcggttg gtggtgccca cctcacggag gcgcgcgccg
     1621 ctcgtctcca ggatctcggg gatccggaac gacccgccga tctcgatcag ttcgccgcgg
     1681 gagatgacca cctcgcggcc ccgggccagc gcctggagcg caagcacgag ggcgccggcg
     1741 gcgttgttga ccgccagtcc gtcctccgcg cccgagagct cggccaggag cccgcggctg
     1801 tggtcgcccc ggtggccgcg gatcccggtg gggaggtcga actcgagggt ggaatacccg
     1861 cccgccaccg cggcgatggc ggcctgggcg ctctcggcga gcggcgcgcg gccgaggttg
     1921 gtgtgcagaa cgacgccggt ggcattgagc accgggacga gcgagggcgc ttcgcgttcc
     1981 accagccgct cccggatggc gagggcccaa tcatccggca cgcccgcgcg cttgcgcgcc
     2041 ccctccagca cctcgcgcac cgcggcgacg accgcgctcc gcggcaccgt atccagcagg
     2101 gcggaaacct ccggctcctt gaggaggcgg tcgacactgg ggagcgcgcg gcgcggatcg
     2161 gtcacggctt ggtggagtcc gcaggcggcg gcaccggccg ggcccggagg tgtcgcttca
     2221 gcgaatcgag gcgctcagcc gtcgagtcgg gcggggtcgg taaggcggct cccggccgga
     2281 gcttcctggt cgagtcggcc ttggtcgagt cggctttggt cgagtcgatg gcgggccgct
     2341 tggggggcgg gggcggcttc ggcaccacga tcccgcccac gatcctgcct cccacgcggt
     2401 tcacgttgcg gatcccggtg acctcgatga cgtacttccc ctggaccttg aggggcgtcg
     2461 ccgtgcggag gaccagccgg tcggtgagcg gtggacgggt cgcgatgagc ttgatcatcg
     2521 cgctgtcgac gcgggggcga ccgcggaggc cggcgaggag ggagggcgcg ggctgcgcgg
     2581 ggggtgccgg cggcagcgcc gccttcttcc ggccggtgtc gggatgggcc ttggcgatcg
     2641 agtcggcgcg gatcgaatcc gccacggccc gggcccgggc ccggagggag tcgtcgatgc
     2701 gcttgggaag gagcgacgcc accgtgaccg gcgtcgaatc ggggagcgcc agcagccgga
     2761 cggtcaccga atcgaagtcg atgtacgggt cgagcggctg gttcaacccg agcgtcaccg
     2821 tctggctgtc ggtcacctgc ggctccacga accggggcgg cgtggtgtcg cgcggggcca
     2881 tccagaaggg cgggacgttg acgctgtccc ggtcggccac gatgctcacg ctgtcccacg
     2941 gctcgcggta tgcgcgcttg aggtcgtggt tctggtcgat gtaggcgtag atgtggtagc
     3001 ggccccgcgg gagggggccg aaggagaagc gaccggtcga gtcgctcgcc gagcggtaca
     3061 cgaggctgtc gggcgccagg accgcctcga ccatcgccac cggcgcggcg cgcccgccca
     3121 cccagtcgat cgtggtcccc cggagcgtca cgtccgggag cggggcgccg gtggtgaagg
     3181 tgatgacgtt cccctgcttc atcacgttcc gccggaggtc ggcgacgccg ggaaggagct
     3241 ccgcccggta cacccgcccc ggcttccatc cttccttcgg ccgcaccgag atcatcccgc
     3301 ggtgccagcg gaccaccggc tgcttcgtgg tgggcgagag gagcaccagc ctctcgagcg
     3361 acccggtgcc gaggccgaag tcggcgctcg taccctcgga gaccgtctcg tcgaagatga
     3421 actcgacgtc gcccttgaaa tcggggagca ccgccgtcga ctccggcacg gtggacacga
     3481 tgaccggcgg cttgaggtcg ggcgggccgc cggggggcgg ggcgatgcgg gcgcaggcgg
     3541 agaggagggc gagaaggcag agcgccacct caccccccaa ccccttctcc cttacgggag
     3601 cgggggagca actctgatgc cttcccccct ctcccataag ggagaggggg acagggggtg
     3661 aggtggtcac ccgcacccca gccgcttcct cttttcctcc agcaccgcga cctgcctgct
     3721 ccagtcggac agcttggtgc gctcattctc gaccacggcg gcgggcgccc gcgccacgaa
     3781 ctgcccgttg gcaagcttcc gttcctgcgc cgccacgagc ccctggagcc gggtgcgctc
     3841 ctccgccagg cggccgcact cccgcgcgag gtcgatggcc ccgcccagcg gcacgaagac
     3901 gggcgtgcca tccgacagca ccgcgtgggc gccgaccccc tgctcggccc gccccagcga
     3961 gagcgatgcg agcttggcga gccgcgagac cgtccccgcc tccgctgcca gcgcggcctc
     4021 tgcatccggg ctcgccgggg tgatgaccgc ggcgatctgc gtgccgggct cgatcccgta
     4081 ttcggcacgg atctgccgga tggccccgac cgcctcctgc accagggcga actgccggag
     4141 cgcggcggga tcggcggcgc ggcggtcgga gccgggccac gcggccgcgg cgagccactc
     4201 gccgtccgcg cggccgggga gcttctgcca gagctcctcg gtgatgaacg gcatcaccgg
     4261 gtggagcagg cggagcgcgg tgtcgaacac ccggtacgcc accgcgcgcg ccacatcgcc
     4321 gccgggctgg tcgccg
//
LOCUS       DASUAP010000008         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_940623, whole genome shotgun sequence.
ACCESSION   DASUAP010000008 DASUAP010000000
VERSION     DASUAP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940623"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2548)
                     /locus_tag="VFU45_00300"
     CDS             complement(<1..2548)
                     /locus_tag="VFU45_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5357527.1"
                     /translation="MVISDFAIKRPIVTVTAMIALVAFGVASLLNLQTDEFPVISQPV
                     VVVTIAYPGASPATVEREVVEPIEEGIFAISGLDGEKTTATATDGLAQITAFFSFEKP
                     IDQASQDIRDAISARRSDLPTEMKEPVLTRIDPGDFPVMQLALTSDSLTPAQLTRIAD
                     PAITRAIRGIPGVAEATVVGGVKRELTVELHAAALQAAGVSVADVVNALNAQNLAAPV
                     GRITSGLTETAIRLAGRPQRPEDFYDLVVTERNGQPIRLGQLADVADGHEEPRTEALF
                     NEHEAVGIDIKKTREASTTDVSRRIHEAVDRITPTLPKGVRFSVVQDAGVRVSNAVRH
                     VEEALVEGALLTVIVVFLFLNSWRSTVITGLALPVSVLASFIAVWAFGFTLNTMSLLG
                     LSLSIGILIDDAIVVRENIVRHVELGMDHYQAAFEGTDEIGLAVAATTFSIVAVFVPV
                     AFMYGIAGQWFKPFALTIACSVLVSLFVSFSLDPMLSAYWPDPELEAHQKGPVTRVLE
                     RFNLWFNRQADRYQGVIAWALDHRLAMVGIATGTFVLAIVMIGAGHPGGGLVPDSDRS
                     EINLIVEAPPGSSLEYTRQKVDEVVAILRRHPEVAYTYSTLGTQVFLKAPSVNQALVY
                     ARLTPRASRAIAQKDLGRILRRELERLGGADISVFTSGFGGAMKQVQLQLRGPDMAVL
                     TDLAQRIEAEVKKAPGAVDVGLSVEGNKPEIQVDLNRGLAGSLGITVGQVALSLRPAF
                     AGLHAGDWVDPAGETRDVEVRLAPGSRDRPSDLAALPLVVGRGGNGAPITVPLGQVAD
                     IRQGVGPGQINHLDREKVINVEANVEGSLNQVMQGIMARVSKVQLPPGYDL"
     gene            complement(2548..3678)
                     /locus_tag="VFU45_00305"
     CDS             complement(2548..3678)
                     /locus_tag="VFU45_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5357528.1"
                     /translation="MDRMKAGPVAYAALTMVLVAGGCGKAAPGTSAAALDPVAVGPEN
                     VTVLTRDTLRSGPTVSGALAAEQEADLRAEVGGRVLRVLADPGQPVRQGQLLVQLDDA
                     AIRDAWLSARSGVTAAQSTADQAHRDEERDRRLADAGAIAARDLEAAHRGSLAADAAL
                     ADAQARLANAQLQLDRTQVRAPFGGLVSDRPANAGDVVQVGAPLVTVVDPVGMRLEAQ
                     VPVDQLAAVHLGTPVLFSVSGLGDRRFTGRIDRINPSVDPVTRQVRIRVSIPNSGHAL
                     VSGLYAQGRVSTVTTVGLSAPPAAVDLTGPAPVVRRLRGGKVELLEVRIGVRDDLAGR
                     VELIGSLAAGDTLLLGSAQALSAGTAVRVLPADAGAPRRQVP"
BASE COUNT          545 a         1404 c         1387 g          548 t
ORIGIN      
        1 agagatcgta gcccgggggg agctggacct tcgagacccg cgccatgatc ccctgcatca
       61 cctggttgag cgatccttcg acgttggcct cgacgttgat caccttttcc cgatcgaggt
      121 ggttgatctg tcccggcccg acgccctggc ggatgtccgc cacctgcccc agcgggacgg
      181 tgatcggggc gccattgccg ccccgcccca ccacgagcgg cagcgcggcg aggtccgacg
      241 gccggtcgcg cgaacccggc gcgaggcgca cctcgacgtc gcgcgtctca ccggcggggt
      301 ccacccagtc gccggcgtgg agcccggcaa aggcgggccg cagcgacagc gccacctgcc
      361 ccaccgtgat cccgagcgac cccgcgagcc cccggttgag gtccacctgg atctcgggct
      421 tgttcccctc gaccgacagg ccgacgtcca ccgccccggg cgccttcttc acttcggcct
      481 cgatgcgctg ggccaggtcg gtaagaacgg ccatgtcggg tcccctgagc tggagctgga
      541 cctgcttcat cgctccgccg aacccgctcg tgaacaccga gatgtcggcg ccgccgagcc
      601 gctcgagttc ccggcgcagg atgcggccaa ggtccttctg cgcgatggcc cgggacgccc
      661 tcggcgtgag ccgcgcgtac accagcgcct gattgacgct cggcgccttg aggaacacct
      721 gggtgccgag ggtggagtag gtgtacgcca cctcggggtg ccgacggagg atggccacga
      781 cctcgtcgac cttctgccgg gtgtactcga ggctcgagcc gggaggcgcc tccacgatga
      841 ggttgatctc gctccggtcg ctgtcgggga cgaggccgcc cccggggtgc cccgcaccga
      901 tcatcacgat cgcgagcacg aaggtgccgg tcgcgatccc caccatcgca agccggtggt
      961 cgagcgccca ggcgatgacc ccctggtagc ggtcggcctg gcggttgaac cagaggttga
     1021 agcgctccag gacgcgggtg accggtccct tctggtgcgc ctcgagctcc gggtcgggcc
     1081 agtaggccga gagcatcggg tcgagggaga agctcacgaa gagcgacacc agcaccgagc
     1141 aggcgatggt gagcgcgaac ggcttgaacc actggcccgc gatcccgtac atgaacgcca
     1201 ccgggacgaa caccgccacg atcgagaacg tggtggccgc cacggcgagc ccgatctcgt
     1261 cggtcccctc gaaggcggcc tggtagtggt ccatccccag ctcgacgtgg cgcacgatgt
     1321 tctcgcgcac cacgatggcg tcgtcgatca ggatgccgat cgagagcgag agcccgagga
     1381 gcgacatcgt gttgagcgtg aagccgaagg cccagaccgc gatgaaactc gccagcacgc
     1441 tcaccggcag tgccaggccg gtgatgaccg tcgagcgcca ggaattgagg aacaggaaga
     1501 ccacgatgac cgtgagcagc gcgccctcga cgagcgcctc ctcgacgtgc cgcaccgcgt
     1561 tggacacccg gacgccggcg tcctgcacca cgctgaagcg cacccccttc gggagcgtcg
     1621 gggtgatgcg gtcgaccgcc tcgtggatgc ggcgcgagac gtccgtggtg ctcgcctcgc
     1681 gggtcttctt gatgtcgatg ccgacggcct cgtgctcgtt gaagagcgcc tcggtgcggg
     1741 gctcctcgtg tccatctgcg acatcagcga gctggcccag ccggatcggc tggccgttcc
     1801 gctcggtcac cacgaggtcg tagaagtcct ccgggcgctg ggggcggccc gcgagccgga
     1861 tggccgtctc ggtcaggccg ctggtgatcc gccccaccgg tgccgcgagg ttctgggcgt
     1921 tgagggcgtt gaccacgtcg gccacgctca cccccgccgc ctggagcgcc gcggcgtgca
     1981 gctccacggt cagctcccgc ttgaccccgc ccaccacggt ggcctcggcc acgccgggga
     2041 tcccccggat ggcgcgcgtg atggccggat cggcgatgcg ggtgagctgt gccggcgtga
     2101 gggagtcgga ggtgagcgcc agctgcatca ccgggaaatc gccgggatcg atgcgggtga
     2161 gcaccggctc cttcatctcg gtcggcaggt cggaccggcg ggcggagatc gcgtcgcgga
     2221 tgtcctgcga ggcctggtcg atcggcttct cgaagctgaa gaacgccgtg atctgcgcca
     2281 gcccgtcggt cgcggtggcg gtggtcttct cgccgtcgag gccgctgatg gcgaagatcc
     2341 cttcctcgat cggctcgacc acctcgcgtt cgacggtggc cggtgaggcg ccggggtacg
     2401 cgatggtgac caccaccacg ggctgcgaga tgaccgggaa ttcgtcggtc tgcaggttga
     2461 ggagcgacgc cacgccgaag gccacgagcg cgatcatcgc cgtgacggtg acgatcggcc
     2521 gcttgatggc gaagtcggag atgaccatca gggcacctgg cggcgcggcg caccagcatc
     2581 ggcgggcagc acccgcacgg cggtgccggc gctgagcgcc tgggcgctcc cgaggaggag
     2641 cgtgtccccg gcggcgagcg acccgatcaa ctcgacgcgg ccggccaggt catcgcgcac
     2701 cccgatgcgc acctcgagca gctcgacctt cccgccgcgg agccggcgca ccaccggcgc
     2761 ggggccggtg aggtccacgg ccgccggcgg cgccgagagc cccacggtgg tgacggtgct
     2821 cacccggccc tgcgcgtaga gccctgacac gagcgcgtgg ccggagttgg ggatgctcac
     2881 ccggatgcgg acctggcggg tcacgggatc gaccgacggg ttgatccggt cgatccggcc
     2941 ggtgaagcgc cggtcaccga ggccgctcac cgagaacagc accggcgtgc ccaggtggac
     3001 ggccgcgagc tgatccaccg ggacctgggc ctcgagccgc atgccgacgg gatcgaccac
     3061 ggtcacgagc ggagcgccga cctgcaccac gtcgcccgcg ttggcggggc ggtcgctcac
     3121 cagcccgccg aacggcgcac ggacctgcgt ccggtcgagc tgcagctggg cgttggcgag
     3181 ccgcgcctgc gcgtcggcca gtgcggcgtc ggccgcgagc gacccgcggt gcgccgcctc
     3241 gaggtcgcgc gcggcgatgg cgcccgcatc ggccaggcgg cggtcgcgct cctcgtcgcg
     3301 gtgggcctgg tcggccgtgc tctgggcagc ggtgacgccg ctccgggccg agagccacgc
     3361 gtcccggatc gccgcgtcgt cgagctgcac gaggagctgg ccctgacgca ccggctgacc
     3421 cggatcggcc agcacccgga gcacccggcc gcccacctcg gcccgcaggt cggcctcctg
     3481 ctccgccgcc agcgcccccg acacggtcgg cccgctccgg agcgtgtccc tggtcagcac
     3541 ggtcacgttc tccggcccca ccgcgaccgg atcgagcgcc gcagcggacg tgcctggcgc
     3601 cgccttgccg cacccgcccg cgaccagcac catcgtcagg gccgcgtatg cgaccggtcc
     3661 cgccttcatc ctgtccatga tccccacgtt cgttgtcatt gtgccgcgcc ggtcactggc
     3721 cgaggggcag gtcgcccagc agggcgatcc tgatccgcgc cagttgcagg tcgcgcgccg
     3781 cctgggcccg cgtcgcctgg gcctgctgca gctgcagccg ggcgtcgctc agctcggtct
     3841 gggtcgagat cccctcgcgg tagcgcacct cggcgatccc gtac
//
LOCUS       DASUAP010000009         4380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_286486, whole genome shotgun sequence.
ACCESSION   DASUAP010000009 DASUAP010000000
VERSION     DASUAP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286486"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFU45_00310"
     CDS             <1..583
                     /locus_tag="VFU45_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEU5357529.1"
                     /translation="TLRATGFFNSHPYLAGVAAGAEARAEYDGVDGGQVERLRAALST
                     PLGALGDQLFWAGVGPAVLGAALIAVALGAGWVVPVGAVAAMLVVRLRVTRWALRTGL
                     RSGMQVGAALQHTRLRSLIPGFGLAAAFAVGAAVPLVVREAAGVRGWPVALAAAVAVA
                     GVAVGQRRLVRLTGVGFGLAAFVVVVLVRLAVG"
     gene            580..846
                     /locus_tag="VFU45_00315"
     CDS             580..846
                     /locus_tag="VFU45_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEU5357530.1"
                     /translation="MITREAPIVNPLGLHARPAAQFVKLASTFGSEIEVHKDGLSVNG
                     KSIMGVMMLAAECGSTITITANGTDEAAAVEALLALVARGFGEA"
     gene            843..2600
                     /gene="ptsP"
                     /locus_tag="VFU45_00320"
     CDS             843..2600
                     /gene="ptsP"
                     /locus_tag="VFU45_00320"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU5357531.1"
                     /translation="MSAQRMIKGIGVSPGVAYAPAVVVRWDLPDVPDREVGAAEVEAE
                     VARLRAAVQETVTALEQLQERVKERAGVEESRIFEAQVMLAQDEDFLRSVEHLIRKNR
                     LSAETAFEYKALEVRNLWANSTSTRLRDRLADLGAIQIRMLGILTGKPAEAVWSGPAD
                     GAEQVIVITHELSPGLTVQLDRDHVVGLISEEGTRTSHAAILAHSLGIPAVMGAAGAL
                     ERIKPGTMVLLDGQAGTLHLEPGPAELEEARVRLSRRQKTELELEAAISQPAESPDGR
                     RYTLLGNVDLPEEIQPALRHGAGGVGLLRTEFLVTGRTHLPTEEEQYAYFRRVTEAFA
                     GRPVVIRSFDLGGDKFPAAFHAPHEPNPFLGWRSIRVCLDEPDLFRPQLRAVLRVAVD
                     RDVQLMLPLIMEVDEVLATKRLLAEEADRLAAAGVRAARSVPVGVMIETPAAVALADQ
                     LARVSAFFSVGSNDLTQYTLAVDRGNARLADRFAPHHPAVVRQLARVVQAGADAGIPV
                     SVCGEMASEPLTAVLLAGLGYTTLSVAPPALPLVKWVLRTVPHAAARTAATAALDASA
                     PGEVLALLQQAADPYLSRR"
     gene            2671..3894
                     /gene="metK"
                     /locus_tag="VFU45_00325"
     CDS             2671..3894
                     /gene="metK"
                     /locus_tag="VFU45_00325"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU5357532.1"
                     /translation="MALQKRHVFTSESVTEGHPDKVADQISDAVLDAILTLDPNGRVA
                     CETLVTTGMAVVAGEITTHAYVHIPDIVRRTVEAIGYTDGSHGFDFKTAAVLSSIDRQ
                     SPDIAMGVNSARGKAQGAGDQGMMFGYATDETRERMPLPILLAHRLAERLAAVRKGGG
                     RLPHLPWLRPDGKSQVSVEYEGDRPVAVRTVVVSTQHAETHRGRELTHRQIQEAIARE
                     VIRPVLEAHGLESRKVRFLVNPTGRFVIGGPHGDAGLTGRKIIVDTYGGMARHGGGAF
                     SGKDPSKVDRSAAYAMRWVARNIVDAGLARRCEVQVAYAIGVAEPVSVFIDTFGTGQM
                     DDRRLERLVRDTFDLTPAGIIRALKLKNPIFGPTAAYGHFGRKPFERQVAGKRREFFT
                     WERSDRAAALRAAAR"
     gene            3901..>4380
                     /locus_tag="VFU45_00330"
     CDS             3901..>4380
                     /locus_tag="VFU45_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU5357533.1"
                     /translation="MIEVVVAHLGLDRTTNAPVVILREKEGARMVPIWIGPAEASAIA
                     MELQGFKPPRPMTHDLLRQVLIGLGADLQRVVVTSVKGNTYFAELHLRRGDDRVQVDA
                     RPSDGIALAVRTHAPIFTSDELLAEAGAAPSGPAPEQRLDADQLKRYLENLDPEDFGK
                     "
BASE COUNT          601 a         1560 c         1612 g          607 t
ORIGIN      
        1 gaccctccgg gcgaccggct tcttcaattc ccatccgtac ctggccggcg tggccgcggg
       61 ggcggaggcg cgggcggagt atgacggcgt cgacggcggc caggtcgagc gcttgcgcgc
      121 cgcgctcagc accccgctcg gggcgctcgg cgaccagctc ttctgggcgg gcgtcgggcc
      181 ggcggtcctc ggcgcggcgc tcatcgccgt ggcgctcggc gccgggtggg tggtgccggt
      241 cggcgccgtg gcggcgatgc tcgtggtgcg gctccgggtg acccgatggg cgctccgcac
      301 cgggctccgg tcggggatgc aggtgggtgc cgcgctgcag cacacccggc tgcggtcgct
      361 gatcccgggg ttcggcctgg cggcggcctt tgcggtcggc gccgcggtgc cgctggtggt
      421 gcgcgaggcc gcgggcgtgc ggggctggcc ggtggcgctg gcggcggcgg tggcggtggc
      481 gggtgtggcc gtcgggcagc gccggctggt gcggctcacc ggcgtcggat tcgggctcgc
      541 ggcgttcgtg gtcgtcgtcc tcgtgcgcct ggcggtcgga tgatcacgcg cgaggcgccg
      601 atcgtgaacc cgctcgggct ccacgcccgg cccgcggccc agttcgtcaa gctcgcgagc
      661 accttcggtt ccgagatcga agtccacaag gacgggctca gcgtcaacgg caagagcatc
      721 atgggggtca tgatgctggc ggcggagtgc ggcagcacga tcaccatcac ggccaacggc
      781 acggacgagg cggcggcggt cgaggcgctc ctcgcgctcg tggcccgcgg gttcggtgaa
      841 gcatgagcgc ccagcggatg atcaagggga tcggggtctc gcccggggtg gcctatgcgc
      901 ccgcggtggt ggtgcgctgg gacctgcccg acgttcccga tcgcgaagtc ggcgccgccg
      961 aggtcgaggc ggaggtggcc cggctccgcg ccgcggtgca ggagaccgtg acggcgctcg
     1021 agcagctcca ggagcgcgtc aaggaacgcg ccggggtcga ggagagccgg atcttcgagg
     1081 cgcaggtgat gctcgcccag gacgaggact tcctccgctc ggtggagcac ctcatccgga
     1141 agaaccggct gagtgcggag acggccttcg aatacaaggc gctcgaggtc aggaacctct
     1201 gggccaactc gaccagcacc cggctccgcg accgcctggc cgatctcggt gccatccaga
     1261 tccgcatgct cgggatcctc accgggaagc ccgcggaggc ggtgtggagc ggccccgcgg
     1321 acggggcgga gcaggtgatc gtgatcaccc acgagctctc gccggggctc accgtgcagc
     1381 tcgaccgcga ccacgtggtg gggctcatca gcgaggaggg gacccgcacc tcgcacgcgg
     1441 ccatcctggc gcactcgctc gggatcccgg ccgtgatggg cgccgccggc gcgctcgagc
     1501 ggatcaagcc cggcacgatg gtcctcctcg atggccaggc gggaacgctg cacctcgaac
     1561 ccggccccgc cgagctcgag gaggcgaggg tccggctgag ccgccgccag aagaccgagc
     1621 tcgagctcga ggcggccatc tcccagccgg cggagagtcc cgacggtcgg cgctataccc
     1681 tgctcggcaa cgtggacctt cccgaggaga tccagcccgc actccgacac ggcgccggcg
     1741 gggtggggct cctgcggacc gagttcctgg tgaccggccg gacccacctg cccaccgagg
     1801 aggagcagta cgcctacttc cggcgggtca ccgaggcatt cgccgggcgg ccggtggtga
     1861 tccggtcatt cgacctgggg ggtgacaagt tcccggccgc cttccacgcc ccgcatgaac
     1921 cgaatccgtt ccttggctgg cgctcgatcc gggtctgcct cgacgagccg gacctcttcc
     1981 ggccccagct gcgcgcggtg ctccgggtgg cggtcgaccg cgacgtgcag ttgatgctgc
     2041 cgctgatcat ggaggtggac gaggtgctgg ccaccaagcg gctgctggcc gaggaggcgg
     2101 accggctggc ggcggcgggc gtgcgggcgg cacggtcggt gccggtgggg gtgatgatcg
     2161 agacgccggc ggccgtggcg ctcgccgacc agctcgcccg cgtcagcgcc ttcttcagcg
     2221 tcggctccaa cgacctgacg cagtacacgc tggccgtcga ccggggcaat gcgcgcctgg
     2281 ccgaccgctt cgcgccgcac catccggcgg tagtgcgcca gcttgcacgc gtggtgcagg
     2341 cgggcgccga cgccgggatc cccgtgagcg tctgcggcga gatggcgtcc gagccgctta
     2401 ccgccgtcct gctcgcgggg cttggctata cgaccctcag cgtggcgccg ccggcgctcc
     2461 ccctcgtcaa gtgggtgctg cgcacggtgc cgcacgccgc cgcgcgcacg gcggcgacgg
     2521 ccgcgctcga cgcgagcgca cccggcgagg tgctggcact cctccagcag gcggccgatc
     2581 cgtacctcag tcgccgctag cggagcgccc gccgccgcga ctagcttgct cccgtcctcg
     2641 cagttcccga cactccagcc agatcggacc atggccctgc agaagcgcca cgtcttcact
     2701 tccgagtcgg tcaccgaagg tcatccggac aaggtggcgg accagatctc cgacgccgtg
     2761 ctcgacgcca tcctcacgtt ggatcccaac ggacgggtgg catgcgagac cctcgtcacc
     2821 accgggatgg cggtcgtggc cggcgagatc accacccacg cctacgtcca catccccgac
     2881 atcgtccgcc gcacggtcga ggccatcggc tacaccgatg gcagccacgg cttcgacttc
     2941 aagaccgcgg cggtgctctc gtcgatcgac cgccagtccc ccgatatcgc catgggcgtc
     3001 aactccgccc ggggcaaggc gcagggcgcc ggcgatcagg ggatgatgtt cggatatgcc
     3061 accgacgaga cccgcgagcg gatgccgctg ccgatcctcc tcgcgcaccg actggccgag
     3121 cgcctggcgg cggtgcggaa gggcgggggt cggctcccgc acctcccgtg gctcaggccc
     3181 gacggcaagt cccaggtgtc ggtggaatac gagggcgacc ggccggtggc ggtccgcacc
     3241 gtggtggtct ccacccagca cgccgagacc caccggggcc gcgagctgac gcaccgccag
     3301 atccaggagg ctatcgcccg ggaggtcatc cgcccggtgc tggaggcgca cggcctcgag
     3361 agccggaagg tgcgcttcct cgtcaacccg accgggcgct tcgtgatcgg cggcccccac
     3421 ggcgacgccg ggctcaccgg gcgcaagatc atcgtcgaca cctacggcgg catggcgcgc
     3481 catggcggcg gcgccttcag cggcaaggat ccctccaagg tggaccggtc ggccgcgtac
     3541 gcgatgcgat gggtggcccg caacatcgtc gacgccgggc tggcgcggcg ctgcgaggtg
     3601 caggtggcct acgccatcgg ggtcgccgag ccggtgtcgg tgttcatcga caccttcggg
     3661 accggccaga tggacgatcg ccggctcgag cggctcgtcc gcgacacctt cgacctgacg
     3721 ccggcaggca tcatccgcgc gctcaagctc aagaacccga tcttcgggcc caccgcggcc
     3781 tacgggcatt tcggccgcaa gccgttcgag cggcaggtgg cggggaagcg gcgggagttc
     3841 ttcacgtggg agcggagcga ccgggcggcg gccctgcggg ccgcggcgcg ctgaccatcg
     3901 atgatcgagg tcgtggtggc ccacctcggg cttgaccgaa cgaccaacgc gccggtggtc
     3961 atcctgcgcg agaaagaagg cgcgcggatg gtgccgatct ggatcggacc cgccgaggcg
     4021 agcgccatcg cgatggaact ccagggcttc aagccccccc ggccgatgac ccacgacctc
     4081 ctgcgccagg tgctcatcgg cctgggcgcc gatctccagc gcgtcgtggt cacctccgtc
     4141 aagggcaaca cgtacttcgc ggagctccac ctgcgccggg gcgacgaccg tgtgcaggtg
     4201 gacgcccgcc cgtccgacgg gatcgcgctc gcggtccgga cccacgcccc gatcttcacc
     4261 tccgatgaac tcctcgctga agccggcgcc gcgccgtcgg ggccggcccc ggaacagcgc
     4321 ctcgatgccg accagctcaa gcgctacctc gagaacctcg accctgaaga ctttggcaag
//
LOCUS       DASUAP010000010         5258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_313772, whole genome shotgun sequence.
ACCESSION   DASUAP010000010 DASUAP010000000
VERSION     DASUAP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313772"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFU45_00335"
     CDS             complement(<1..431)
                     /locus_tag="VFU45_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5357534.1"
                     /translation="MTPPGIKVLVADDHTVVREGIRHILERAPGFHVVAEAANGADAL
                     RLAGSTLPDVAVLDVSMPGMSGLKVAAELRRAVPEARVLILSMYDNPEYVLEAVRAGA
                     HGYILKDSAATDLRKAVEAVHAGESFFSPVIAKRLSEAERG"
     gene            complement(428..2206)
                     /locus_tag="VFU45_00340"
     CDS             complement(428..2206)
                     /locus_tag="VFU45_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEU5357535.1"
                     /translation="MQYAIPPATAQEFSAGALQALVALALALLCLHLALRYHRAYFRW
                     WAAAWALYLARILCILSFLATQHWSWLYWHQVITGWTALAILYAALVFSRNLHWSPRH
                     ALYALFPPVWSYIAIYAMRDFFWAALPMVLFLSFATVWTGWVFLQYRRRVGGSAGATV
                     LATTFLLWGVHHLDYPVLRERGTWVPWGYYVDVLFFLGAGSGILLLVLDELRRGLAAL
                     SALSGDLQQGGAGDRLDALLGRPLSLPGVRGSALVRALADGEQQVVRGAGGCAGWTGV
                     VPPGPGAAAIRAALGSGRPEITNQWSGEGVLGTPNGYVAILPVFSGARATGALLIAGE
                     ARDPFAVLDTAFLVALGHQVGTALEYGGLTARLEARTAELERLSSRMVRLHEDERRRL
                     SRELHDETAQVLSAVKMQLGLLRERSDPAAVLELDRALALLDAGIRGIRNLTNDLRPS
                     LLDDLGLLPALRSLTAEFAGRSGTVVEFRAPEELPTLSEEAELAIFRALQEALANVAR
                     HAGARVVKVELVAAGAGLSLDVVDDGPGFGRVDLGRLEQEGHMGLVGMRERIAALGGK
                     LELSGEDEGARVHIDVPLPRDTGRSR"
     gene            complement(2298..2951)
                     /locus_tag="VFU45_00345"
     CDS             complement(2298..2951)
                     /locus_tag="VFU45_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357536.1"
                     /translation="MRTLSTLLLLTLTAAPLAAQQHKDMKDPDKAVQGGGTLPTGWSA
                     RTDKNAPLTNVKFVTMGTGLHATMGPAAIFWQPGDTATGDFHTLATFTQMKAPMHPEA
                     YGLIIGGSDLSGAGQRYTYLIVRGDGKYMIKRRNGDSVTTVVPWTANDAVVKAGADGK
                     ATNEVSIQVKGGTVSFMVNGKEVHSGSAADFDTKGIVGLRVNHNLDVHIAGFAVHHI"
     gene            3058..3228
                     /locus_tag="VFU45_00350"
     CDS             3058..3228
                     /locus_tag="VFU45_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357537.1"
                     /translation="MFQAIYVTLAFVGAIGIICALAFVTLLIGVLVNSMVTLMSGPLI
                     DPLLKKYKPEWL"
     gene            complement(3286..3648)
                     /locus_tag="VFU45_00355"
     CDS             complement(3286..3648)
                     /locus_tag="VFU45_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357538.1"
                     /translation="MAVKLSSKQQAQLAFLEPYGRQLTAMGSLIEQMAAPKADEQLGR
                     TLLRQVSASKTQAQGLGLTRLADAMGGIEQVARRTGGGVSKFRTLRELYQMLKINYEA
                     ALKVASTAEAHAEEESGA"
     gene            complement(3726..4055)
                     /locus_tag="VFU45_00360"
     CDS             complement(3726..4055)
                     /locus_tag="VFU45_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357539.1"
                     /translation="MTEGSGRAAPRGARSPLLDTLGQSIQNGKELASYLWQVPGDVDA
                     HRQIEALVATIRKESAAQGRREMGRVCDEIERAAGASPTPQQVELLQDGFERLYKLWL
                     AARSGIM"
     gene            4096..>5258
                     /locus_tag="VFU45_00365"
     CDS             4096..>5258
                     /locus_tag="VFU45_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEU5357540.1"
                     /translation="MRRSLLPLAALVFVLSLPRAAEAQLTRPERTAFKETSSYADVMA
                     FLDSLRLRGAQFREGVMGLSPEGKPIPYVVVSRPLVSTPVEAARSGKPVIYIQANIHA
                     GEIEGKEAAQMLLRDLTLGALRPLLDSVVIVMAPIYNSDGNDRWAPGEVNRPGQNGPA
                     LVGTRANGQGYDLNRDYVKLEAPETRGSLALLTAWDPDVFLDLHTTDGSYHGYALTYS
                     PGLNPNSPPANDYVRDWFLPRIRERVRQRHHEEMFWYGNFRDQDPDSLTQGWETYDPR
                     PRFGTNLFGMRGRISILSEAYSNDDLRTRITSTYDFVHEILSLLAEDRTRVKAVIARS
                     DTFHPDSVTVRSVMAPPTMQDVIAELTRPAGEGSGPYARRTRTGVFRTIRMPV"
BASE COUNT          771 a         1890 c         1816 g          781 t
ORIGIN      
        1 tggccgcgtt cggcctccga gagccgcttg gcgatgaccg ggctgaagaa cgactcgccg
       61 gcgtggaccg cctccaccgc cttccggagg tccgtggcgg cgctgtcctt gaggatgtag
      121 ccgtgggcgc ccgcccggac ggcctccagc acgtactccg ggttgtcgta catgctgagg
      181 atgaggacgc gggcctccgg gacggcgcgc cggagttccg ccgccacctt gaggcctgac
      241 atccccggca tcgacacgtc gaggacggca acgtcaggga gggtcgagcc ggccagccgg
      301 agcgcgtcgg cgccgttggc ggcctcggcc acgacgtgga atcccggcgc ccgctcgagg
      361 atgtgccgga tcccctcgcg caccacggtg tggtcgtcgg ccacgagcac cttgatcccc
      421 ggcggcgtca tcgggacctc cccgtgtccc ggggcagggg gacgtcgatg tggacccgcg
      481 ccccttcgtc ctcgccgctc agctcgagct tcccgccgag cgccgcgatc cgttcccgca
      541 tgccgaccag tcccatgtgc ccttcctgtt cgagtctgcc gaggtcgacc cggccgaagc
      601 cggggccgtc gtccaccacg tcgagggaca gcccggcgcc tgccgccacg agctccacct
      661 tcaccacccg cgcccccgcg tgacgcgcca cgttcgcgag cgcctcctgc agcgcccgga
      721 atatggcgag ctcggcctcc tccgagaggg tcggcagctc ctccggcgcg cggaactcga
      781 ccacggtccc gctgcgcccg gcgaattcgg ccgtgagcga ccgcagcgcg gggaggagtc
      841 ccaggtcgtc gaggagcgac ggccggaggt cgttggtgag gttccggatg ccccggatcc
      901 cggcgtcgag cagcgccagt gcgcggtcga gctcgagcac ggcggccggg tcggagcgtt
      961 cccggaggag gccgagctgc atcttgaccg ccgagagcac ctgcgccgtc tcgtcgtgca
     1021 gctcccgcga caggcggcgg cgctcgtcct cgtgcaggcg caccatccga ctggagaggc
     1081 gttcgagctc ggcggtgcgg gcctcgagcc gcgcggtgag gccgccgtac tcgagcgccg
     1141 tgccgacctg gtggcccagc gccacgagga aggcggtgtc gagcacggcg aacgggtcgc
     1201 gcgcctcgcc ggcgatgagg agcgcgccgg tggcccgggc accgctgaac accggcagga
     1261 tggcgacgta cccgttcggc gtccccagca cgccctcgcc ggaccactgg ttggtgatct
     1321 ccgggcgccc cgatccgagg gccgcgcgga tcgccgcggc acccgggccg ggcggcacga
     1381 ccccggtcca cccggcgcac ccgccggcgc cgcggaccac ctgctgctcg ccgtcggcca
     1441 gcgcccggac cagcgcgctg ccccgcaccc ccggaaggct caaggggcgt ccgaggagcg
     1501 cgtcgaggcg gtcgccggcg ccgccctgct ggaggtcgcc cgagagcgcc gacagcgccg
     1561 cgagtccgcg gcgcagctcg tcgagcacga gcaggaggat cccgctgcct gcgccaagga
     1621 agaagagcac gtcgacgtaa tagccccacg ggacccaggt accgcgctca cggagcaccg
     1681 ggtagtcgag gtggtggacc ccccacagca ggaaggtcgt ggcgagcacc gtcgcacccg
     1741 ccgacccgcc cacccgccgc cggtactgca ggaagaccca gccggtccag acggtggcga
     1801 agctcaggaa cagcaccatc ggcagcgcgg cccagaagaa gtcccgcatc gcatagatgg
     1861 cgatgtagga ccagaccggc gggaagagcg cgtagagcgc gtggcgcggg ctccagtgga
     1921 ggttccgcga gaagacgagc gcggcgtaga ggatcgcgag cgcggtccag cccgtgatga
     1981 cctggtgcca gtagagccag ctccagtgct gggtggccag gaacgagagg atgcagagga
     2041 tgcgcgcgag gtagagtgcc cacgccgcgg cccaccagcg gaagtacgcc cggtggtagc
     2101 ggagcgccag gtgcaggcag aggagcgcga gcgccagcgc caccagcgcc tggagggcgc
     2161 cggccgagaa ctcctgtgcc gtggcggggg ggatcgcgta ttgcatcggt ggggaatcta
     2221 ccgccggacc cggaatgcgg tcgtgggccg cgggtcaccc ccacgaccca cgacccacga
     2281 ctcacgaccc acgactctca gatgtgatgt accgcgaacc cggcgatgtg gacgtcgagg
     2341 ttatggttga cccggagccc gacgatgccc ttggtgtcga agtccgccgc gctgccgctg
     2401 tggacctcct tgccgttcac catgaaggac acggtgccgc ccttgacctg gatcgacacc
     2461 tcgttggtcg ccttgccgtc ggcgccggcc ttgacgaccg cgtcgttggc cgtccacggg
     2521 accaccgtgg tgaccgagtc gccgttgcgg cgcttgatca tgtacttccc gtcgccccgg
     2581 acgatcaggt aggtgtagcg ctggccggcg ccgctcaggt cgctcccgcc gatgatcagc
     2641 ccgtacgcct ccgggtgcat cggcgccttc atctgcgtga aggtcgcgag ggtatggaag
     2701 tcgccggtgg cggtgtcgcc cggctgccag aagatcgcgg ccggccccat cgtggcgtgg
     2761 agcccggtgc ccatcgtgac gaacttcacg ttcgtgagcg gggcgttctt gtcggtccgg
     2821 gcggaccacc cggtgggcag ggtgccgccc ccctggaccg ccttgtcggg gtccttcatg
     2881 tccttgtgct gctgggccgc caggggtgcg gcggtaaggg tcaggaggag cagggtggac
     2941 agggtgcgca tgggatgacc tcgctcaggt ggagggacgc gggccgcggt tgcggggcgt
     3001 tcccggttcg actagactga cttccgatct gggagacccg aacaccgggg agggacgatg
     3061 ttccaggcca tctatgtcac gctcgccttc gtcggcgcca tcggcatcat ctgcgcgctg
     3121 gcgttcgtca cgctgctcat cggcgtgctg gtcaattcga tggtcacgct gatgtccggg
     3181 cccctcatcg atccgctgct caagaaatac aagcctgaat ggctctgacc cggacctgcg
     3241 gacgctgacg gccggaccac ctggtccggc cgttctcatt ccccgtcagg ccccggactc
     3301 ctcttccgcg tgtgcctccg cggtcgaggc caccttgagc gccgcctcgt agttgatctt
     3361 gagcatctgg tagagctcgc ggagcgtgcg gaacttgctg accccgccgc cggtgcgtcg
     3421 tgcgacctgc tcgatcccgc ccatcgcgtc ggccagccgg gtgagcccga gcccctgcgc
     3481 ctgggtcttc gaggccgaca cctggcggag gagcgtgcgg cccagctgct cgtccgcctt
     3541 gggcgccgcc atctgctcga tcagcgagcc catcgccgtg agctggcggc cgtacggctc
     3601 caggaacgcc agctgggcct gctgcttgga actgagcttg accgccatgt tcgggccttt
     3661 cgggggacct gccggggatg gccggccggt ggccggcacc cgggaaagat actcgacccg
     3721 ccgccctaca ttatgcccga ccgcgccgcc agccagagct tgtacaggcg ctcgaacccg
     3781 tcctgcagca gctccacctg ctgcggcgtc ggagaggcgc cggccgcccg ctcgatctcg
     3841 tcgcagacgc ggcccatctc ccgccgcccc tgggcggccg attccttccg gatcgtggcg
     3901 acgagggcct cgatctggcg gtgcgcgtca acgtcgccgg ggacctgcca gaggtaggag
     3961 gcgagctcct tgccgttctg gatcgattga cctagcgtgt cgagcagcgg ggagcgtgcc
     4021 cctcggggcg cggcccggcc tgatccttcc gtcatgagcg tttctctccc aggaattgga
     4081 acggaaagct aaccgatgcg tcgatccctc ctgccgctcg cggccctggt ctttgtgctt
     4141 tccttgcccc gggccgcgga ggcgcagctc acccgacccg aacgcaccgc cttcaaggag
     4201 acctcctcct acgcggacgt gatggcgttc ctcgactcgc tccggctccg cggcgcgcag
     4261 ttccgggaag gggtcatggg actgagcccc gaaggcaagc cgatcccata tgtggtcgtc
     4321 tcccgccccc tcgtgtcgac tccggtcgag gcggcccgga gcgggaagcc ggtgatatac
     4381 atccaggcca acatccacgc cggcgagatc gaggggaagg aagcggccca gatgctgctc
     4441 cgcgacctga ccctgggcgc cctgcgcccg ctcctcgaca gcgtcgtgat cgtcatggcg
     4501 ccgatctaca acagtgacgg gaacgaccgg tgggcccccg gggaggtcaa ccggccgggc
     4561 cagaacggcc ccgccctcgt gggcacccgc gccaacggcc aggggtacga cctcaaccgc
     4621 gactacgtca agctcgaggc ccccgagacc cgcgggtcgc tggcgctcct cacggcgtgg
     4681 gatcccgacg tcttcctcga cctccacacc accgacggca gctatcacgg ctacgccctg
     4741 acgtattcgc cgggcctcaa ccccaacagc cccccggcca acgactacgt gcgcgactgg
     4801 ttcctgcccc ggatccgcga gcgcgtgcgc cagcggcatc acgaggagat gttctggtac
     4861 ggcaacttcc gcgaccagga tcccgactcc ctcacccagg gctgggagac gtacgatccc
     4921 aggccgcgct tcggcaccaa cctgttcggg atgcggggac ggatctcgat cctgagcgag
     4981 gcgtacagca acgacgacct ccggacccgg atcacctcga cctacgactt cgtccacgag
     5041 atcctctcgc tccttgccga ggaccgcacg cgggtcaagg ccgtcatcgc ccggtcggac
     5101 actttccatc ccgactccgt caccgtgcgc tcggtgatgg cgccgcccac gatgcaggat
     5161 gtgatcgcag aactgacccg gccggcgggc gaggggagcg gtccctatgc ccgtcgtacc
     5221 cgtaccggtg tcttcaggac catccggatg ccggtctt
//
LOCUS       DASUAP010000011         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_272828, whole genome shotgun sequence.
ACCESSION   DASUAP010000011 DASUAP010000000
VERSION     DASUAP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272828"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFU45_00370"
     CDS             complement(<1..300)
                     /locus_tag="VFU45_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357541.1"
                     /translation="MVVMLTATAGLSAWYAAYSPLSAVPIVGFTALAVFLEQGSTRLS
                     LSAMGSVVFVIHISATILFGPIEGAIIAAASTAVSEMLFRRAPIKAVFNTAQKTLA"
     gene            complement(653..1375)
                     /locus_tag="VFU45_00375"
     CDS             complement(653..1375)
                     /locus_tag="VFU45_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357542.1"
                     /translation="MTGSASAREPGDDKEPRTSSPDVWGVRRAENLLTSLSGVLSARV
                     VTTPLGEVSEVHILAQAGVAAKQIVRNVESALLAQLGLKVDHRKISVAQTAEVQPIQA
                     VEQEAVKQRALQRAVLFEGMQVGPGRKNRVSVEVTLGFNGRTASVSEEATDTPKGRLE
                     AAGRAAAAVVDELLGDHSIALDGVKLVAAFDQEVVVAGVQGLGGREPVLLVGTAVVRE
                     SAEMAAVLAVLDATNRWADVRR"
     gene            complement(1383..>2677)
                     /locus_tag="VFU45_00380"
     CDS             complement(1383..>2677)
                     /locus_tag="VFU45_00380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5357543.1"
                     /translation="AVEREYPELVPYLAPVTTPAEAEARYLRALHGPDVPLVYAGVVP
                     PSGKEVLDAAITFADLGELFERRGVDPAGQPDVFVRLPEERRRYLSLAGGMPLALLAE
                     VGKPGARRVRTIRGLEALPAIARAVRAGQDLGFVDLLSHEGTLDHPIAGPKEDLFWRR
                     SVVEASEPPRSRFPVVDAGVVASIGATFDIRPRKRPVNEVFVGAILDAIGTGPNGRAW
                     DCRACGFPSCRRFAEAASEGRATLRQCTPYQVRRADEAQRQAAVDSLTGLATYRVLRD
                     RLAAEIERSKRSGEKVAIVFLDLDRFKDLNDRWGHETGNRVLERLGRELQGGVRGSDL
                     AARYGGDEFVILLPRTDLSGGQRVAETLRRRLEGVGPELGLPAGSVTASLGVAEHDPA
                     APAAGDLIELADRALYAAKTAGRNVVAPAEAGDRDQST"
BASE COUNT          381 a         1107 c          795 g          394 t
ORIGIN      
        1 ggcgagggtc ttctgggcag tattgaagac tgccttgatc ggcgctcgcc ggaacagcat
       61 ttccgagacg gccgtcgacg cagccgcgat aatggcacct tcgatcgggc cgaataggat
      121 cgtcgctgaa atatggatca caaagacgac cgaccccatc gcactcaggg agagcctggt
      181 gctcccttgt tccaggaaga ccgcaagtgc cgtaaaacca acgatcggca cggcactcag
      241 cggtgaatag gcggcatacc acgcactaag gcccgccgtg gcggtgagca tcaccaccac
      301 ggctacgaag atccggacct tagtttctgg tctcatttcg tacggcgaag gaggaatccc
      361 ggtcttcacg cacttcctgg acgcatcaga cccaggacac agtgtcggtc gtgaaaaggc
      421 gagcgacaaa atgaatcgcc gccgcgagcc aagtgatatg cagcatcgag cgtcacctcc
      481 tttcgggtac cactgacctg ccgaaagtgc caagcctcgc tatggcctga ccgacgctcc
      541 gctcatccct ggtccgctcg tctccgctcc cagcttcgcg aagaccgggg cctcctccat
      601 cctccttccg aagttccgct ccgcccgttg tgggcccttc ccggcgcgcg cgctaccgcc
      661 gcacgtcggc ccatctgttg gtggcgtcga gcaccgcgag caccgccgcc atctcggcgc
      721 tctcccgcac caccgccgtc cccaccagca agaccggctc ccgccccccc agcccctgca
      781 cccccgccac caccacttcc tggtcgaacg ccgccaccag cttgacgccg tcgagcgcga
      841 tggagtggtc gcccagcagt tcgtcgacga ccgccgccgc cgcccgcccc gccgcctcca
      901 gccgcccctt cggcgtgtcg gtggcctcct ccgacacgct ggcggtccgc ccattgaagc
      961 cgagggtcac ctcgacggac acccggttct tccgccccgg ccccacctgc atcccctcga
     1021 agagcaccgc ccgctgcagc gcccgctgct tcacggcctc ctgctccacg gcctggatcg
     1081 gctgcacctc ggcggtctgc gccacgctga tcttccggtg gtccaccttg agcccgagct
     1141 gcgccagcag cgccgactcc acgttccgca cgatctgctt ggccgccacc ccggcctgcg
     1201 ccaggatgtg gacctcgctc acctcgccca gcggcgtcgt gaccacccgc gccgagagca
     1261 cccccgagag gctggtgagc aggttctccg cccggcgcac cccccacacg tcgggggagg
     1321 aggtcctggg ctccttgtcg tctcccggct cacgcgccga tgccgatccg gtcactgacc
     1381 cctcacgtcg attggtcccg gtcgccggcc tcggccggcg ccaccacgtt ccgtcccgcc
     1441 gtcttggccg cgtagagcgc gcggtcggcc agttcgatca ggtcccccgc cgccggcgcc
     1501 gccgggtcgt gttcggccac gcccaggctc gccgtcacgc tccccgccgg gagccccagc
     1561 tccggcccca caccttcgag ccgccgccgg agggtctccg ccacccgctg cccgccgctc
     1621 aggtcggtgc gcgggagcag gatcacgaat tcatcgccgc cgtagcgcgc cgccaggtcg
     1681 gacccccgca ctcccccctg cagctcgcgc cccagccgtt ccagcacccg gttgccggtc
     1741 tcgtgccccc accggtcgtt caggtccttg aaccggtcga ggtcgaggaa gacgatggcc
     1801 accttttcgc cgctgcgctt gctgcgctcg atctccgccg ccagccggtc gcggagcacc
     1861 cggtacgtcg cgagccccgt aaggctgtcg accgcggcct gccgctgcgc ctcgtcggcc
     1921 cggcgcacct ggtacggcgt gcactgccgg agcgtcgccc ttccctccga ggctgcctcg
     1981 gcaaagcgcc ggcagctcgg gaacccgcag gcccggcagt cccaggccct gccgttcggc
     2041 cccgtcccga tggcgtcgag gatggcgccc acgaacacct cgttgaccgg ccgcttccgg
     2101 gggcggatgt cgaacgtcgc cccgatgctc gccaccaccc cggcgtccac caccgggaac
     2161 cggctccgcg gcggctcgct cgcctccacc accgaccggc gccagaagag atcctccttg
     2221 ggccccgcga tcggatggtc gagcgtcccc tcgtggctca gcaggtccac gaagccgagg
     2281 tcctgccccg cccgcaccgc ccgcgcgatg gcggggagcg cttccaaccc ccggatggtc
     2341 cgcacccgcc gggcgccggg ctttcccacc tccgccagga gcgccagcgg catccccccc
     2401 gccaggctca ggtaccgccg ccgctcctcc ggcagccgga cgaagacgtc gggctgtccg
     2461 gccggatcca ccccgcgccg ctcgaagagc tcacccaggt cggcaaaggt gatcgccgca
     2521 tcgagcacct ccttgccgga gggcggcacc acccccgcat acaccagcgg cacgtcgggc
     2581 ccgtgcagcg cccgcaggta cctcgcctcc gcctcggccg gcgtcgtcac cggcgccagg
     2641 tacggcacca gctccggata ctcccgctcc accgcct
//
LOCUS       DASUAP010000012         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_54796, whole genome shotgun sequence.
ACCESSION   DASUAP010000012 DASUAP010000000
VERSION     DASUAP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54796"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="VFU45_00385"
     CDS             <1..1471
                     /locus_tag="VFU45_00385"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEU5357544.1"
                     /translation="GPLDVRVQADSADVEMLEAFTPNARDLTGSFSADAGIQGTWDDP
                     RLTGTVGVRDAGVSLPALGVRYDRITGAARLSGDSITLDSLSLRGGEGSLAVRGGVRL
                     ERLTRPVFDLDLRAQGFRAMDVRNYLTLEASGQLRLTGPFVGATLTGRMTADQGVLYF
                     ADLVTKQVVDLSDPAIAALLDTALVRDARLEPSIENRFLDSLGIDNLRLAVGDEFWLR
                     STEANIRLTGDMTVSKAARNYRLDGTLKADRGNYALKVGFITRPFTVQSGTVRFLGTP
                     DLNAELDIQAVHTVRPADASPDIPVVAHITGTTLAPKLALSSPQTPTLSETELVSYLM
                     FGRPSFALDQTAGTGVGGEQAALQTGIAYLSSALSTEIERALVSDLGVPVDFVEIRPG
                     GAGGSFVNPNAGLTTLSAGWRIGKRTFFTFNAGLCPNLGQLSYKSFGASIEQRLDPWL
                     RAQVSVEPVRTCQPNVAVDALGATNQYQLGFDLLWEREY"
     gene            1473..2384
                     /locus_tag="VFU45_00390"
     CDS             1473..2384
                     /locus_tag="VFU45_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU5357545.1"
                     /translation="MTRALVITNPAAARTDRDAVRVVERILRRGGWQADVLVTGGGGD
                     ARRYAAEGVRAGVDVVAVFGGDGTTMQAAAALVGTGVALGVLPGGTGNLLAGNLRIPT
                     HPQRAAELLLESRPRAFDLGRMERQDGTHFFAVACGAGFDARVMEETPAAFKRQWGMG
                     AYIATTLKILPEVRSTDTWITVDGTEHRLRSAMVLVANCGEVIPPLIRLRSGISPRDG
                     LLDVIAIRADSVGQSVLALWDLLREVAVDDDDRYVTHLRGREISVRAAEALPVQLDGE
                     MGGLTPFAVQVVPNAIQVMVPEGTTLP"
     gene            2387..>4447
                     /locus_tag="VFU45_00395"
     CDS             2387..>4447
                     /locus_tag="VFU45_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5357546.1"
                     /translation="MSAPATLPVEILAAVAQATAELAPPERVYDTLRVQTERLLETDA
                     FYLALWDEDRQRIHFVAHHDGGVRLAPSETPLGSGPTSWVIRNRRSYRLHGQGDPVHE
                     AANFFGDHRRSGSALHVPLQVGDRLVGVISAQAYRAGAYTADSQRLLEALAAHGAIAL
                     EVGRIARRTRDLDHEVRRRLDQLDAVGQVAHLLATVDDAQRTMEAAAREGMKVFDATR
                     CGLLLIDQATGRIEAPVSVGLSSRYLDAVAERFHQIPVAPTIMAGEAVFFEDMPSAPD
                     SPVVTEAYAEGFRTLAVLPLVYSGETIGLLAFMHDAPHIWSPAERRVAATFADQAALA
                     IGKSRLLDRVTQVKSEWQAVFDAAPSGLAVLDREGRIQRANRWIADLAGVAVTELPGL
                     ELRVVTSEWPAGGADPLERARSTGTPVTALIPGRRGRLLVLTVAPQPDGRAVAALDDV
                     TEVVRTEARYRALFRAAPVAIVTLDREGRFQSVNDAATGLFGAESSPRRLIDAVVPAE
                     RGHVQAVLAASFRGERRDFIFHVPQAGGGVREAQGVAVPVEERGGVATVLVLARDVTD
                     EVDLRERVGHSEKMAALGQLVSGVAHELNNPLAGIAALSQALLSDGSGDDGTDRVLHS
                     IRGEAERAGRIVKDLLAFARQRPIERREVDLNAVVRAALAGPNVHPDRWQLDLADALP
                     PVSGD"
BASE COUNT          576 a         1668 c         1582 g          621 t
ORIGIN      
        1 cggtccgctc gacgtccggg tccaggccga cagcgcggac gtcgagatgc tcgaggcgtt
       61 cacgcccaac gcgcgggacc tgaccggcag cttcagcgcc gatgcaggca tccagggcac
      121 ctgggacgat ccgcggctta ccggcaccgt cggcgtccgg gacgccgggg tgtcgctgcc
      181 cgccctcggg gtccggtacg accgcatcac cggcgccgcg cgcctgagcg gcgacagcat
      241 cacgctcgac agcctctcgc tccgcggcgg cgaggggtcg cttgcggtgc gcggcggcgt
      301 gcggctggag cggctcaccc gcccggtgtt cgatctcgac ctccgggcgc agggcttccg
      361 cgcgatggac gtccggaact acctcaccct cgaggcgtcg ggccagctcc ggctgaccgg
      421 gcccttcgtc ggtgcgacgc tcaccggacg gatgaccgcc gaccaggggg tgctctattt
      481 cgccgacctc gtgaccaagc aggtcgtcga cctgagcgac ccggccatcg cggccctcct
      541 cgacacggcc ctggtgcggg atgcgcggct cgagccgtcg atcgagaacc gcttcctcga
      601 ctcgctcggc atcgacaacc tccgccttgc cgtcggcgac gagttctggc tccggtccac
      661 cgaggccaac atccggctga ccggcgacat gacggtgagc aaggccgccc gcaactatcg
      721 cctcgacggc acgctcaagg ccgaccgcgg caattacgcg ctcaaggtcg ggttcatcac
      781 ccggccgttc accgtgcagt cgggaacggt ccggttcctc ggcacgcccg acctgaatgc
      841 cgagctcgac atccaggcgg tgcacaccgt gcgtcccgcc gacgcgagtc ccgacatccc
      901 ggtcgtggcg cacatcaccg gcaccacgct ggcgcccaag ctggcgctct ccagtccgca
      961 gactcccacg ctgtcggaga cggagctcgt gtcctacctc atgttcgggc gcccgtcgtt
     1021 cgcgctcgac cagaccgccg gcaccggggt gggcggggag caggccgcgc tgcagaccgg
     1081 tatcgcttat ctctccagcg cgctctcgac cgagatcgag cgcgcgctcg tgtcggacct
     1141 gggcgtgccg gtggacttcg tggagatccg ccccggcggc gccggcggca gcttcgtcaa
     1201 cccgaacgcc gggctcacca cgctctcggc cggctggcgc atcggcaagc ggacgttctt
     1261 caccttcaac gcggggctct gccccaacct cggtcagttg agctacaaga gcttcggcgc
     1321 cagcatcgag cagcgcctcg atccctggct ccgggcgcag gtcagcgtgg agccggtgcg
     1381 gacctgccag cccaacgtgg cggtggacgc gctcggcgcg accaaccaat atcagctggg
     1441 gttcgacctc ctctgggaac gggaatactg acgtgacgcg cgccttggtc atcaccaatc
     1501 cggccgccgc ccgcaccgac cgcgatgcgg tgcgggtggt cgagcgcatc ctccgccgcg
     1561 gcggctggca ggccgacgtc ctcgtcaccg gcggcggcgg agacgcccgg cgctacgccg
     1621 ccgagggcgt ccgggccggc gtcgacgtcg tcgccgtctt cggtggcgac ggcaccacga
     1681 tgcaggccgc tgcggcgctg gtcggcaccg gggtggccct cggcgtcctc cccggcggga
     1741 ccggcaacct cctcgccggg aacctgcgca tcccgaccca tccccagcgc gcggccgagc
     1801 tgctcctcga atcgcgcccc cgcgccttcg atctcggccg gatggagcgc caagacggca
     1861 cccacttctt cgcggtggcg tgcggggcgg ggttcgacgc ccgcgtgatg gaggagacgc
     1921 cagccgcgtt caagcgccag tgggggatgg gggcgtacat cgccaccacg ctcaagatcc
     1981 tgccggaagt gcggagcacc gacacctgga tcaccgtgga cggcaccgag caccggctcc
     2041 gttccgcgat ggtgctggtg gccaactgtg gggaggtcat cccgccgctc atccggctcc
     2101 gcagcggcat ctcgccccgc gacggcctgc tcgacgtgat cgcgatccgc gccgacagcg
     2161 tgggccagag cgtgctggcg ctctgggacc tgctccgcga ggtcgcggtg gatgacgacg
     2221 accggtacgt gacgcacctg cggggccgcg agatctccgt gcgggctgct gaggccctcc
     2281 cggtgcagct tgacggcgag atgggcggcc tcacgccgtt cgccgtccag gtggtcccga
     2341 acgccatcca ggtgatggtg cccgagggga ccaccctccc gtgacgatgt ccgcgccggc
     2401 cacgctcccc gtggagatcc tggcggccgt cgcccaggcc acggcggaac ttgccccgcc
     2461 ggagcgggtc tacgacacgc tccgggtcca gaccgagcgg ctgctcgaga ccgatgcgtt
     2521 ctaccttgcc ctctgggacg aggaccggca gcgcatccac ttcgtggccc accacgacgg
     2581 cggcgtgcgc ctggcgccgt cggagacgcc gctgggcagc gggccgacga gctgggtcat
     2641 ccggaaccgc cgttcgtacc ggctccacgg ccagggcgac ccggtgcacg aggctgccaa
     2701 cttcttcggc gaccaccggc gttccgggtc cgcgctccac gtgccgctcc aggtcggcga
     2761 ccgcctggtc ggcgtgatct cggcgcaggc ataccgggcc ggggcgtaca ccgccgattc
     2821 gcagcggctc ctggaggcgc tcgcggccca cggcgccatc gcgctcgagg tcggccgcat
     2881 cgcgcgccgc acccgggacc tcgaccacga ggtccggcgc cgcctcgacc agctcgacgc
     2941 cgtgggccag gtggcgcacc tgctggccac cgtggacgac gcccagcgta cgatggaggc
     3001 ggcggcccgc gaggggatga aggtcttcga cgccacccgc tgcgggctgc tgctcatcga
     3061 ccaggccacc ggccggatcg aggcgccggt ctcggtgggg ctctcgtccc gctacctcga
     3121 cgcggtggcc gagcggttcc accagatccc ggtggcgccc acgatcatgg ccggcgaggc
     3181 ggtcttcttc gaggacatgc cctccgcccc cgattcgccg gtggtcacgg aggcctacgc
     3241 cgagggattc cgcaccctgg cggtgctgcc gctcgtctac tccggcgaga ccatcggcct
     3301 cctcgcgttc atgcacgatg cgccccatat ctggtccccg gctgagcgca gggtcgcggc
     3361 gacctttgcc gaccaggccg cgctcgccat cggcaagagc cggctcctcg accgcgtgac
     3421 ccaggtcaag tcggagtggc aggccgtctt cgacgccgcg ccgagcggct tggccgtgct
     3481 cgaccgggag gggcggatcc agcgggccaa ccggtggatc gccgacctgg cgggcgttgc
     3541 ggtcacggag cttcccgggc tcgagctccg ggtggtcacc tccgagtggc ccgccggcgg
     3601 cgccgacccg ctcgagcggg cgcggagcac cggcaccccg gtcaccgccc tcatcccggg
     3661 gcgccggggc cggctgctcg tcctcacggt ggcgccgcag ccggatggcc gcgcggtcgc
     3721 ggccctggac gacgtcaccg aggtcgtgcg caccgaggcg cggtaccgcg cgctcttccg
     3781 cgccgcgccg gtcgcgatcg tgaccctcga ccgcgaggga cggttccagt ccgtcaacga
     3841 cgcggccacc gggctcttcg gtgctgagtc ctccccccgc cgcctgatcg acgcggtggt
     3901 gccggcggag cggggtcacg tccaggcggt gctggccgcg agcttccggg gcgaacgccg
     3961 cgacttcatc tttcacgtgc cgcaggcggg cggcggcgtt cgcgaggcgc agggtgtggc
     4021 ggtgccggtg gaggagcggg gcggcgtggc gaccgtgctg gtcctggcgc gcgacgtgac
     4081 cgatgaggtc gacctgcgcg agcgggtggg acacagcgaa aagatggccg cgctgggcca
     4141 gctcgtgtcc ggggtggccc acgagctcaa caacccgctc gccggcatcg ccgcgctctc
     4201 gcaggcgctc ctctccgacg gctccggcga cgatggcacc gaccgggtgc tccactccat
     4261 ccgcggcgag gccgagcggg cgggccgcat cgtcaaggac ctgctggcct tcgcgcggca
     4321 gcgcccgatc gagcggcgcg aggtggacct gaatgccgtg gtgcgcgcgg cgctggcggg
     4381 ccccaacgtc cacccggacc ggtggcagct cgacctcgcc gacgccctcc cgccggtgag
     4441 cggcgac
//
LOCUS       DASUAP010000013         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_381927, whole genome shotgun sequence.
ACCESSION   DASUAP010000013 DASUAP010000000
VERSION     DASUAP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381927"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..360)
                     /locus_tag="VFU45_00400"
     CDS             complement(28..360)
                     /locus_tag="VFU45_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5357547.1"
                     /translation="MAFSAAKTNGVMVVHVEGQLIVGNRQELKQLVQEALDHGERKFL
                     IDFAKAGYIDSSGLGALVSISKKVRETGGELRLSGLNDDLRSLFELTKLDTLFAINDG
                     LTQALAGF"
     gene            complement(375..1310)
                     /locus_tag="VFU45_00405"
     CDS             complement(375..1310)
                     /locus_tag="VFU45_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEU5357548.1"
                     /translation="MTFQEDLLVRAARIRPRLVFPEGGEPRVVEALREVERRGIARPI
                     VIGGAGGIIPAEDPRLGRVARHLRERRPDKVPDDATATALATDPLRFAAGLVGIGEAD
                     GCVSGAVATTGDVIRAGLLALGTAPGIPLVSSSFYMAFPDGRVFTFTDCAVVPDPCAD
                     QLAAIAWAAAAARRALVGDEPRVAFLSYSTKGSAAGPRVEKMQEAARLFREQHPEVVS
                     DGELQGDAALVAEVAGRKAPGSPIAGNANVLVFPDLDAGNIAYKLVQRLGGATAIGPI
                     LQGMARPMSDLSRGAVARDIVEVAAMVALQCTPTP"
     gene            complement(1307..1810)
                     /gene="coaD"
                     /locus_tag="VFU45_00410"
     CDS             complement(1307..1810)
                     /gene="coaD"
                     /locus_tag="VFU45_00410"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU5357549.1"
                     /translation="MTHDPRPTPRTAIYPGSFDPPTRGHEDLIRRALAFCDRLIVAVA
                     VNQNKQPLFSVEERLAMLRSIAGGDKRIEVASFEGLLADYAQKRGATLVLRGLRAVSD
                     FEYEFQMALMNRQLHAELETVFLVPAVDLTYLSSSLVREVARYGGNISGLVHPEVAAA
                     LARKFTK"
     gene            complement(2174..2779)
                     /gene="rsmD"
                     /locus_tag="VFU45_00415"
     CDS             complement(2174..2779)
                     /gene="rsmD"
                     /locus_tag="VFU45_00415"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU5357550.1"
                     /translation="MPPARSSPAARPLPAHCARMRVISGTFGGRRLKMPKGDQVRPTA
                     DRVREAWFSILAAAVPGARVLDLFAGSGALGLEALSRGAAHAEFVERHRLSLAALREN
                     IAALGVEARTIVRPVEAMKFAAALAPGAFDLAFADPPYATPHAAELVALFRKTPFARI
                     LSVEHAASVALDGDDTRRYGDTAVTFLWAEGAGSGVASHGS"
BASE COUNT          439 a         1022 c          975 g          376 t
ORIGIN      
        1 aggcggaggg ggctggtcac ggcaacgtca gaacccggcg agcgcctggg tgagcccgtc
       61 gttgatggca aagagggtgt cgagcttggt gagctcgaag agcgaacgga ggtcgtcgtt
      121 gagccccgag agccgcaact caccgccggt ctcccgcacc ttcttggaga tcgacaccag
      181 ggcgccgagg ccggaggagt cgatgtaccc ggccttggcg aagtcgatca ggaacttccg
      241 ctcgccgtgg tccagcgcct cctggaccag ctgcttgagc tcctgccggt tcccgacgat
      301 cagctgcccc tcgacgtgga ccaccatcac gccattggtc tttgccgccg aaaacgccat
      361 ctgtcccctc cccgtcacgg ggtcggtgtg cactgcaagg cgaccatcgc ggccacctcc
      421 acaatatcgc gcgccacggc cccgcgcgac aggtcgctca tcggacgggc catcccctgc
      481 aggatcgggc cgatggcggt ggccccgccc agccgctgga ccagcttgta ggcgatgttg
      541 ccggcgtcca ggtcgggaaa gacgaggacg ttcgcgttcc ccgcgatcgg cgaccccggc
      601 gccttgcgac cggcgacctc cgccaccagc gccgcgtccc cctggagctc cccgtccgac
      661 accacctccg ggtgctgctc ccggaagagc cgggccgctt cctgcatctt ctccaccctg
      721 ggcccggcgg cactcccctt ggtgctgtag gagaggaacg ccacccgggg ctcgtcgccc
      781 accagcgccc gacgggccgc cgccgccgcc caggcgatcg ccgccagctg gtcggcgcag
      841 ggatcgggca ccacggcgca gtcggtgaag gtgaataccc ggccgtcagg gaaggccatg
      901 tagaaggacg acgagacgag cgggatcccc ggcgcggtgc cgagcgccag gaggccggcc
      961 cggatgacat cgccggtcgt ggccacggcc cccgacacgc acccgtcagc ttcgccgatc
     1021 ccgacgagcc ccgccgcaaa gcggagcgga tcggtggcga gtgcggtggc ggtggcgtcg
     1081 tcgggcacct tgtcggggcg tcgctcgcgc aggtgccggg cgacccgccc caggcgcgga
     1141 tcctcggccg ggatgatccc acccgcaccg ccgatcacga tcggccgcgc gatcccccgc
     1201 cgctcgacct cccgcagtgc ctccaccacc ctcggctccc cgccctcggg gaagacgagc
     1261 cggggccgga tccgcgccgc gcggacgagg aggtcctcct ggaaggtcac ttcgtgaact
     1321 tccgggccag cgccgccgcc acctccgggt gcaccagccc gctgatgttg ccgccgtagc
     1381 gcgccacctc ccgcaccagc gatgagctca ggtacgtcag gtccaccgcc ggcaccagga
     1441 ataccgtctc gagctcggcg tgcagctggc ggttcatcaa cgccatctgg aactcgtact
     1501 cgaagtcgct caccgcgcgg agcccgcgca ggacgagcgt ggccccgcgc ttctgggcgt
     1561 agtcggccag gagcccctcg aagctcgcca cctcgatccg cttgtcgccg ccggcgatcg
     1621 accggagcat cgccaggcgc tcctcgacgg agaagagcgg ctgcttgttc tggttcacgg
     1681 ccacggcgac gatgagccgg tcgcagaagg cgagcgcccg ccggatcagg tcctcgtgcc
     1741 cgcgggtcgg cgggtcgaag gagccgggat agatggcggt gcggggggtg ggtcgtgggt
     1801 cgtgggtcat gggtcgtggg tcgtgggtcg tgagacgtaa gacgtaagac gtaagacgtg
     1861 agacgtgaga cggggtgtgg agttccgatc ctacatccga gtcatcccga ggggccttga
     1921 ggccccgaag gatctcgaac cgataccggc atcgacgccg gaacaccgcg tgcccgaatc
     1981 ggccaggatc aggttgagat ccttcgcttc gctcaggatg actccgtgcg cggccggccg
     2041 ccgctgccgc aaccgagtca tcctgcggga ccttcaggcc ccgacgtgcc agtcccgtgc
     2101 agagcgaggg gatctcgaac cgatgccggt cactgcgccg gaacgcggcg tgcccgaagc
     2161 gatcggccac gactcacgac ccatgactcg cgacccccga tccagccccc tccgcccaca
     2221 ggaacgtaac cgccgtatcc ccgtaccgcc tcgtgtcgtc gccgtcgagc gccaccgacg
     2281 ccgcatgttc caccgagagt atccgcgcaa aaggcgtctt ccggaagagg gcgacgagct
     2341 ccgcggcgtg cggcgtcgca tacggcgggt cggcaaaggc cagatcgaag gccccggggg
     2401 cgagcgccgc cgcgaacttc atcgcctcga cagggcgcac gatggtccgc gcctcgacgc
     2461 cgagtgccgc gatgttctcc cgaagtgccg cgagcgagag ccggtggcgc tcgacgaact
     2521 cggcgtgcgc cgccccgcgc gagagcgcct cgagccccag cgccccgctc cccgcaaaga
     2581 gatcgagcac ccgcgccccc ggcaccgcgg cggccaggat gctgaaccac gcctcccgca
     2641 cccggtcggc ggtggggcgg acctgatcgc ccttgggcat cttgagccgc cggccgccga
     2701 aggtgccgga gattacgcgc atgcgtgcac agtgggctgg cagcgggcga gcagcgggcg
     2761 aggagcgggc ggggggcacg cagcgggctg acagcgggca tacagcgggc ga
//
LOCUS       DASUAP010000014         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_627255, whole genome shotgun sequence.
ACCESSION   DASUAP010000014 DASUAP010000000
VERSION     DASUAP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627255"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFU45_00420"
     CDS             <1..385
                     /locus_tag="VFU45_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357551.1"
                     /translation="WSAAHNSFVQIGAELGVPGLLIFLALLLTAFRTLHRIAVEARRR
                     SGPQAREAALAQALIASLIAYCVAGFFLSEAFSPYLYSVLGMTVGLAKAVASSPAATG
                     GAVAPGRPGTSAGWRASRMIPPPSP"
     gene            490..1659
                     /locus_tag="VFU45_00425"
     CDS             490..1659
                     /locus_tag="VFU45_00425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5357552.1"
                     /translation="MAIRSYHIMRLLAERYDVTALCFIRANERPTPAHVAESVMAISA
                     FAKIEAFPIPQEHSRVRLLWDHLRSVLTRRPYTVYAYESAAFRTRVRELLERLQPDLV
                     HMDSLDLSGYLDLVPAASTVCVHHNVESALLRRRADAEKSWWRRAYLHLQADLLDGEE
                     RRWCPRMGLNVAVSPDDRAIFEQNAPGARWAVVPNGVDVDYYRPGAQGSDGLVCVGAT
                     TWFPNRDALYFFCNEIQPQLKAARNAPARWVGRSTREDQETCRRDFGVELTGYVDDTR
                     PYLERAACFIVPLRVGGGTRLKILNAWAMGKAIVSTSIGCEGLDAMDGVNILIRDTAQ
                     GFAEAIDLVVADEVFRRRLGAEGRRTVERTYSWKIIGRDMEGLYHGVAKRLGSPA"
     gene            1691..>2553
                     /locus_tag="VFU45_00430"
     CDS             1691..>2553
                     /locus_tag="VFU45_00430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5357553.1"
                     /translation="MRPGVAGLLRWMARRAPLILAYHNVVPDDASPSGDRSLHLPRRE
                     FAAHLDILAERCRVVPLADLPDARADDKPVVAITFDDAYRGAVTTAVDELVRRALPAT
                     IFVTPKFVGDGAFWWDELTPPGAVAPAIPRYIALDALCGDDDSIRKWAAEHGVSVTRP
                     PSHSRVATEAELRAAVAKPGITLGSHTWGHPNLAALSYEDTSRELTQSWTWLRDRFPD
                     RLIPWIAYPYGGVSEDVQEVAAATGYRAGLRVDGGRARAGAWRNTPHAVPRYNVPSGI
                     SRRGFRARVGGG"
BASE COUNT          365 a          892 c          871 g          425 t
ORIGIN      
        1 gtggtccgcg gcgcacaatt cgttcgtgca gataggcgcg gagctcggcg tcccggggtt
       61 gctcattttc ctcgccctcc tcctcaccgc cttccgcacg ctgcatcgga tcgccgtgga
      121 ggccaggcga cgatccgggc cccaggcgcg ggaggcggcg ctggcccagg cgctcattgc
      181 gtcgctcatt gcgtactgcg tggcgggatt cttcctgtct gaagcgttct cgccctacct
      241 ctactcggtg ctcggcatga ccgtcgggct cgccaaagcc gtggcgtcgt ccccggcggc
      301 gaccggcggg gctgtggcac ccgggcggcc gggcacgtcc gccggatggc gggcctcccg
      361 gatgatccct ccaccgtccc cgtgaccgcg accagccagc gccagcgggc cggcgccgtc
      421 tctgcaaagg gcgcccgccc cccactcctt ttcctctgcc agacactgcc gtaccccccg
      481 gatggcgggg tggccattcg gtcctatcac atcatgcgac ttctggccga gcgctacgac
      541 gtgacggcgc tttgcttcat acgtgcgaac gagcgcccta cgccggccca cgttgccgag
      601 agcgttatgg cgatctcagc attcgcgaag atcgaggcgt tcccgatccc ccaggagcac
      661 agtcgggttc ggttgctctg ggatcacctt cgcagtgtgt tgacgcgccg gccgtacacc
      721 gtctacgcgt acgagtcggc ggcgttccgg actcgcgttc gcgagctgct cgagcgactg
      781 cagcctgatc tcgtgcacat ggacagcctc gacctgtcgg ggtacctgga cctcgttccc
      841 gcggccagta cggtctgtgt gcatcacaac gtggagtccg cgttgctccg ccgccgggcc
      901 gacgctgaga agtcctggtg gcgacgggcg tatctgcatc tgcaggcgga cctgctcgat
      961 ggcgaagaac gccgctggtg ccctcgcatg gggctgaacg tcgccgtgtc tcccgatgat
     1021 cgcgccatct tcgagcagaa cgctcccggc gcgcggtggg cggtggtgcc gaacggggtg
     1081 gacgtggact actaccggcc gggcgcccaa gggagcgacg gcctggtctg tgtcggtgcg
     1141 acgacgtggt tcccgaatcg ggatgccctg tacttcttct gcaacgagat ccagccgcaa
     1201 ctcaaggcgg cgcggaacgc cccggcccga tgggtcggac ggagcactcg tgaagaccag
     1261 gagacctgcc gtcgagactt cggtgtcgag ctgaccggct atgtcgacga cacccgaccg
     1321 tacttggagc gggccgcgtg cttcattgtt cccctgcgcg tgggcggagg gacacgactc
     1381 aaaatcctga acgcctgggc gatgggcaaa gccatcgtga gcacgtccat cgggtgcgaa
     1441 gggttggacg ccatggacgg cgtgaacata ctgatccggg ataccgcgca ggggtttgcc
     1501 gaagccatcg atctggtcgt ggcggatgag gtgttccgac gccggttggg tgccgaaggg
     1561 cgccggacgg tggagcggac gtacagctgg aaaatcatcg gccgtgacat ggaagggctg
     1621 tatcacggtg tcgcgaagag gttgggcagc cctgcgtaac ctggtcaaaa acgttgccga
     1681 ggctctggcc gtgcggcccg gcgtcgccgg cctcctgcgg tggatggccc gccgcgctcc
     1741 gctgatcctc gcctatcaca acgtggtccc cgatgacgcg tcgccatcgg gagaccgctc
     1801 tctgcacctg ccccgccgcg agttcgccgc gcacctcgac atcctcgccg agcggtgcag
     1861 ggtggtcccc ttggcggacc ttcccgacgc gcgtgcggac gacaagccgg tggtcgccat
     1921 cacgttcgac gacgcctacc ggggcgccgt cacgacggcg gtcgacgagc tggtgcgccg
     1981 cgcgctcccc gccacgatat tcgtcacgcc caagttcgtc ggtgacggcg cgttctggtg
     2041 ggatgagctc accccgcccg gcgcggtggc gccggccatc ccgcgctaca tcgccctcga
     2101 cgccctctgc ggggacgacg actccattcg gaagtgggcg gctgagcatg gcgtgagcgt
     2161 caccaggccg cccagtcact cccgcgtcgc caccgaagcg gagctccgcg ccgccgtcgc
     2221 caaacccggc atcaccctcg gctcccacac ctggggtcac cccaacctgg ccgccctgtc
     2281 ctacgaagac acgtcgcgcg agctgaccca atcgtggacc tggctgcgcg accgctttcc
     2341 ggaccgcctg atcccctgga tcgcgtatcc ctacggcggc gtcagcgagg atgtccagga
     2401 ggtggcggcc gccacggggt accgggcggg gctgcgggtg gacggagggc gcgcgcgggc
     2461 gggagcctgg cggaacacgc cccacgccgt cccgcgctac aacgtgccct cggggatctc
     2521 acgccgcggc ttccgcgccc gcgtcggcgg agg
//
LOCUS       DASUAP010000015         4974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_123048, whole genome shotgun sequence.
ACCESSION   DASUAP010000015 DASUAP010000000
VERSION     DASUAP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123048"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFU45_00435"
     CDS             <1..625
                     /locus_tag="VFU45_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU5357554.1"
                     /translation="LAIDRHVPIGGKDFKTGQTLMKTVLAPMFKARMLGVSGWYSTNI
                     LGNRDGEVLDDPDNFKTKEESKLGVLEYILQPKMYPQLYAKMYHKVRINYYPPRGDNK
                     EGWDNIDIFGWLGYPMQIKVDFLCRDSILAAPLVLDLALFFDLAQRAGMSGIQEWLSF
                     YFKSPQVAKGLYPEHDLFIQQTKLKNTLRWLMGEDPITHLGMEYYQK"
     gene            622..912
                     /locus_tag="VFU45_00440"
     CDS             622..912
                     /locus_tag="VFU45_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357555.1"
                     /translation="MKYFHRTSIAPDAVMARATAFFGARLTPAEEGARRRRFTGTLGH
                     LSVGAEAEGGHYTRITVETDQPGESEADKLAKRFLATVHTLADPGHVLRGAY"
     gene            987..3458
                     /locus_tag="VFU45_00445"
     CDS             987..3458
                     /locus_tag="VFU45_00445"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEU5357556.1"
                     /translation="MKSLEHVRARVRPIGAALAALVALPLALQAQSAGGGEASLKVPD
                     LNTITFLGGLTGHQLLLTGLVICALGFGFGLWMYAHLRDLPVHASMREISELIYETCK
                     TYLVTQGKFLIALWVFIGAVAALYFGELAPTTDATGATVHGYPLGTVVVILLFSLVGI
                     AGSYSVAWFGIRVNTFANSRTAFASLKGKPFPCYDIPLKAGMSIGMLLISVELLMMLG
                     ILLFIPGEYAGPCFIGFAIGESLGAAALRIAGGIFTKIADIGADLMKIVFNIKEDDAR
                     NPGVIADCTGDNAGDSVGPSADGFETYGVTGVALITFILLAVPDVVVQVQLLVWIFVL
                     RVMMIIAAGLSYFINAAIAKARYQQADKLNYEVPLTTLVWLTSLVSVAITYVASYLTI
                     PSVQGDTTLWWKLSTVVTCGTLAGAIIPEFVKVFTSVESRHVREVVTSSREGGPSLNI
                     LSGLVAGNFSAYWLGITMLVLMAIGYLVSGSFPAGIMMAPAVFAFGLVAFGFLGMGPV
                     TIAVDSYGPVTDNAQSVFELSIIETLPNIKAELKKDYGFDVDFEKAKHHLEENDGAGN
                     TFKATAKPVLIGTAVVGATTMIFAIIVALTNGLQPALVANLSILHAPFLLGLICGGAM
                     IFWFTGASIQAVTTGAYRAVEFIKANIKLEGVTKASVEDSKAVVEICTKYAQKGMWNI
                     FLAVFFATLAFAFVEPYFFVGYLISIALFGLYQAIFMANAGGAWDNAKKIVEVELKQK
                     GTPLHAATVVGDTVGDPFKDTSSVALNPIIKFTTLFGLLAVELAVSLSDARGPLLGHV
                     LAAVFFVISAAFVYRSFYGMRIESQ"
     gene            3620..4729
                     /locus_tag="VFU45_00450"
     CDS             3620..4729
                     /locus_tag="VFU45_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5357557.1"
                     /translation="MPPSSSPAQPQKAPPAGLSHDQLLELYAWMRLTRTLEERLVALY
                     RQTKVVGGLFRSLGQEADAVGTALALDRSKGDVLSPLIRNLGSMLVQGATPLEILRQY
                     MARGDSPTRGRELNIHFGSPERGFIGQISHLGDMVPVMAGVTLSFKLRGEPRVGMVYV
                     GDGATSTGAFHEGINLAAVQRCPLVVIIESNGYAYSTPTRRQTAARRFVDKAPGYGIP
                     GFEADGNDVLAVYDVTRKAVDRARQGGGTSLIELHTYRRKGHAEHDNQSYVPPGEIER
                     WAETNDPIDRYIKVLRAEYGVEPEALAAIDARVSAEVDAATDEAERSPFPEPLDALHG
                     VYADPPTAPVLWYREGRAAAVDRNERAQGWGTYHG"
     gene            4722..>4974
                     /locus_tag="VFU45_00455"
     CDS             4722..>4974
                     /locus_tag="VFU45_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5357558.1"
                     /translation="MAEVTYLEAIRQGLVEEMERDPNVFCLGEDIGAYGGAFKVTEGL
                     LDRFGGTRVIDTPISEAAIVGAAAGAAHMGMRPVCEMQFI"
BASE COUNT          777 a         1777 c         1617 g          803 t
ORIGIN      
        1 gctcgccatc gaccggcacg tgccgatcgg cggcaaggat ttcaagaccg ggcagacgct
       61 catgaagacc gtgctggcgc cgatgttcaa ggcgcgcatg ctgggggtct cggggtggta
      121 ttccaccaac atcctcggca accgcgacgg cgaggtgctg gacgacccgg acaacttcaa
      181 gacgaaggaa gagtcgaagc tcggcgtcct cgagtacatc ctccagccga agatgtatcc
      241 ccagctctat gccaagatgt accacaaggt gcggatcaac tattacccgc cccgggggga
      301 caacaaggaa ggctgggaca acatcgacat cttcgggtgg ctcggctacc cgatgcagat
      361 caaggtcgac ttcctgtgcc gtgactcgat cctggcggcg cccctggtgc tcgacctggc
      421 gctcttcttc gacctggccc agcgcgccgg gatgtccggg atccaggagt ggctctcctt
      481 ctacttcaag agcccccagg tggccaaggg gctctatccg gagcacgacc tcttcatcca
      541 gcagaccaag ctcaagaaca cgctgcgctg gctgatgggc gaggacccga tcacccacct
      601 gggcatggag tactaccaga agtgaagtac ttccaccgca cgtcgatcgc gcccgacgcc
      661 gtgatggcgc gggcgaccgc cttcttcggc gcccggctca cgccggccga ggagggcgcg
      721 cgccgccgcc ggttcaccgg gacgctgggg cacctgtcgg taggggcgga ggcggagggg
      781 gggcattaca cgcggatcac ggtcgagacc gaccagccgg gcgagagcga ggccgacaag
      841 ctggccaagc gcttcctcgc cacggtgcac accctggccg acccggggca cgtgctccgg
      901 ggcgcctact gagtcatcga ccgggcgggg ccgccgcccg gccgcacggg gacgcttccc
      961 cgtcacttca ggagaaacgc acgcccatga agtcgctgga gcacgtccgc gcccgcgtcc
     1021 gcccgatcgg cgccgcgctc gccgccctcg tggccctgcc gctcgccctt caggcccagt
     1081 cggccggggg cggcgaggcg agcctcaagg tacccgacct caacaccatc accttcctcg
     1141 gcgggctcac cggccatcag ctgctgctga cgggcctggt catctgcgcc ctcggcttcg
     1201 gcttcgggct gtggatgtac gcgcacctcc gcgacctccc ggtccacgcg tcgatgcgcg
     1261 agatctccga gctcatctac gagacctgca agacgtatct cgtcacgcag gggaagttcc
     1321 tgatcgccct gtgggtgttc atcggggccg tggcggcgct ctacttcggc gagctggccc
     1381 cgaccaccga cgccaccggc gccacggtgc acgggtaccc cctcggcacc gtcgtggtca
     1441 tcctgctgtt cagcctggtc ggcatcgcgg ggagctacag cgtggcctgg ttcgggatcc
     1501 gggtcaacac cttcgccaac agccgcacgg cgttcgcgag cctcaagggg aagcccttcc
     1561 cctgctatga catcccgctc aaggccggga tgagcatcgg gatgctcctg atcagcgtcg
     1621 agctcctgat gatgctcggc atcctgctct tcatccccgg cgagtacgcc ggcccctgct
     1681 tcatcggctt cgccatcggc gagtcgctgg gtgccgcggc gctccggatc gcgggcggca
     1741 tcttcaccaa gatcgccgac atcggcgccg acctgatgaa gatcgtcttc aacatcaagg
     1801 aagacgacgc gcggaacccc ggcgtcatcg ccgactgcac cggcgacaac gccggcgact
     1861 cggtcggccc ctcggccgac ggcttcgaga cctacggcgt caccggcgtc gcgctcatca
     1921 cgttcatcct gctggcggtg ccggacgtgg tggtccaggt gcagttgctc gtctggatct
     1981 tcgtgctgcg ggtgatgatg atcatcgcgg ccgggctctc gtacttcatc aacgccgcca
     2041 tcgcgaaggc ccgctaccag caggcggaca agctgaacta cgaagtgccg ctcaccacgc
     2101 tggtgtggct cacctcgctc gtctcggtgg ccatcaccta cgtggcctcg tacctgacca
     2161 tcccgtcggt ccagggtgac accacgctct ggtggaagct gtcgaccgtg gtcacctgcg
     2221 gcacgctcgc cggggccatc atccccgagt tcgtgaaggt gttcacgtcc gtcgagtcgc
     2281 ggcacgtgcg cgaggtcgtg acctcctccc gcgagggcgg gccgtcgctc aacatcctgt
     2341 cgggcctggt ggccggcaac ttcagcgcct actggctcgg catcacgatg ctggtgctga
     2401 tggccatcgg ctacctcgtg agcggctcct tcccggccgg catcatgatg gcgccggcgg
     2461 tgttcgcctt cggcctggtg gcgttcgggt tcctcggcat ggggcccgtc accatcgcgg
     2521 tcgactccta cggcccggtg accgacaatg cccagtcggt cttcgagctc tccatcatcg
     2581 agacgctccc caacatcaag gcggagctca agaaggacta cggcttcgac gtcgacttcg
     2641 agaaggccaa gcaccacctc gaggagaacg acggcgcggg caacaccttc aaggccacgg
     2701 ccaagccggt gctgatcggc accgccgtgg tcggggccac cacgatgatc ttcgcgatca
     2761 tcgtggcgct caccaacggc ctccagccgg cgctggtggc caacctctcg atcctgcacg
     2821 cgcccttcct gctcggcctc atctgcggcg gggcgatgat cttctggttc accggcgcgt
     2881 cgatccaggc cgtgaccacc ggcgcctacc gcgccgtcga gttcatcaag gccaacatca
     2941 agctcgaggg cgtcaccaag gcctcggtcg aggacagcaa ggccgtcgtc gagatctgca
     3001 ccaagtacgc gcagaagggg atgtggaaca tcttcctggc cgtcttcttc gcgacgctgg
     3061 ccttcgcctt cgtcgagccg tacttcttcg tcgggtacct gatctcgatc gcgctcttcg
     3121 ggctctacca ggccatcttc atggccaacg ccggcggcgc ctgggacaac gcgaagaaga
     3181 tcgtggaggt cgagctcaag cagaagggca cgccgctcca cgccgccacc gtcgtcggcg
     3241 acaccgtggg cgaccccttc aaggacacga gctcggtggc cctcaacccg atcatcaagt
     3301 tcaccaccct cttcggcctc ctggccgtgg agctggcggt ctcgctcagc gacgcccgcg
     3361 ggccgctgct gggccacgtc ctcgccgcgg tcttcttcgt catctcggcg gcgttcgtct
     3421 accggtcgtt ctacgggatg cggatcgaga gccagtgagc ggtgagcggt aagtggtgag
     3481 cggggggctc cggtcggtga cggggccccc cgctgactgc ttccccctca ccgctgaccg
     3541 cgcaccttcc ttgactcccc ccgcccgccg ggaaatcttt ccccgctcga cacttaagcg
     3601 ctctcgagca cggacccgga tgccgccgtc ttcatcgccc gcccagcccc agaaggcccc
     3661 cccggccggg ctctcgcacg accagctcct cgaactatac gcctggatgc ggctcacccg
     3721 gacgctggag gagcggctcg tcgccctcta ccggcagacc aaggtcgtcg gcgggctctt
     3781 ccggtcgctg gggcaggagg ccgatgcggt gggcaccgcc ttggccctcg accggtcgaa
     3841 gggcgatgtc ctctcccccc tcatccgcaa cctcggctcg atgctggtgc agggcgccac
     3901 ccccctcgag atcctccgcc agtacatggc ccggggcgat tccccgaccc gcggacgcga
     3961 gctcaacatc cacttcggct cccccgagcg cggcttcatc ggccagatct cccacctggg
     4021 cgacatggtc ccggtgatgg cgggcgtcac gctctccttc aagctccgcg gggagccgcg
     4081 cgtggggatg gtctacgtcg gcgacggcgc cacctcgacc ggggcgttcc acgaggggat
     4141 caacctggcg gcggtgcagc gctgcccgct ggtggtcatc atcgagagca acggctacgc
     4201 ctactcgacg cccacccgcc ggcagaccgc cgcccgccgg ttcgtggaca aggcaccggg
     4261 ctacgggatc cccggcttcg aggcggacgg caacgacgtc ctcgccgtct acgacgtgac
     4321 caggaaggcg gtcgaccggg cccgccaggg aggcggcacg tcgctgatcg agctgcacac
     4381 ctaccggcgc aaggggcatg cggagcacga caaccagtcg tacgtgccgc ccggtgagat
     4441 cgagcgctgg gccgagacca atgacccgat cgaccgctac atcaaggtgc tgcgcgccga
     4501 gtacggcgtg gagcccgagg cgctcgccgc catcgacgcg cgggtgagcg ccgaggtcga
     4561 cgccgccacc gatgaggccg agcgctcgcc gttccccgag ccgctcgatg cgttgcacgg
     4621 cgtctacgcc gatccgccga cggcgccggt gctctggtac cgcgagggcc gcgccgccgc
     4681 cgtggaccgg aacgagcggg cgcaggggtg ggggacgtac catggctgag gtcacctacc
     4741 tcgaggcgat ccgccagggg ctcgtggagg agatggagcg cgacccgaac gtcttctgcc
     4801 tcggcgagga catcggggcg tacggcggcg cgttcaaggt caccgagggg ctgctcgacc
     4861 gcttcggcgg cacgcgggtg atcgacacgc cgatcagcga ggcggccatc gtcggggccg
     4921 cggccggcgc cgcccacatg gggatgcgac cggtctgcga gatgcagttc atcg
//
LOCUS       DASUAP010000016         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_259351, whole genome shotgun sequence.
ACCESSION   DASUAP010000016 DASUAP010000000
VERSION     DASUAP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259351"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFU45_00460"
     CDS             <1..371
                     /locus_tag="VFU45_00460"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU5357559.1"
                     /translation="RSGAALASLRSLAGFDPARDLLVLVDDVALPAGKFRLRGAGSAG
                     GHNGLKSIEGTLRRQDYPRLRIGVGPVPAGLFDLADFVLAAPSPDDHAAITELLEPMS
                     QAVEVWLSDGIEKAMSLFNR"
     gene            complement(361..801)
                     /locus_tag="VFU45_00465"
     CDS             complement(361..801)
                     /locus_tag="VFU45_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU5357560.1"
                     /translation="MGYFKDLKVWRAAHGLALAVYRATGGFPPAERFGLTAQTRRAAV
                     SVSANLAEGAGRRSDAELVRFAGIALGSLHELEAELLLARDLGWLDVELSASLESDMG
                     RLGRAIAALQRRLRNAGAGARRPLARGSGDLRRTTNDQRPTTTD"
     gene            969..2066
                     /gene="ychF"
                     /locus_tag="VFU45_00470"
     CDS             969..2066
                     /gene="ychF"
                     /locus_tag="VFU45_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU5357561.1"
                     /translation="MLRLGIVGLPNVGKSTLFNALTQAGALVANYPFATIEPNTGVVT
                     VPDPRLDELARIVSPERTVPAAVEFVDIAGLVKGASQGEGLGNQFLANIREVDAIVHV
                     VRCFADDNIQHVMGAVDPARDRDIINSELGLADLAVVEKRLDKAARAAKSGDAVAKVE
                     HALLQRLHPALADGKPARSVVPTDEEAPVFRTLNLLTAKPVLYAANVPEGEINAGNAF
                     VQALAAAVAKDGETAEIVTFSAQVEMELAELAPDERVPFLESLGLTESGLDRLARAAY
                     HLLGLQSYFTAGEKEVRAWTIHRGDRAPAAAGVIHSDFEKGFIRAETVSYADFVRVGG
                     WKGARDQGLARAEGKEYVVQDGDVMLFRFTS"
     gene            2120..2836
                     /locus_tag="VFU45_00475"
     CDS             2120..2836
                     /locus_tag="VFU45_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU5357562.1"
                     /translation="MSDPNLYRSAAPKYAGLDESQALIASFLRRVYGWMFVGLAATAI
                     TAWQVASSEAAVKVIFGTPFLFFALLFGELGLVWWISARVGKMAPSTTAALFVVYSVV
                     NGLTLSVVLLAYTGASIETTFLTAAAMFGALALFGTLTGRNLQGMGQFAMMGLFGLLA
                     AMLVGFFWHSDALQFIISIVGVIVFTGLTAWDAQKLKSMALALEGRESGGYAISGALA
                     LYLDFVNLFLFLLRFLGRRR"
BASE COUNT          446 a         1135 c          952 g          522 t
ORIGIN      
        1 accggagcgg tgccgcactg gcatcgctcc gttcgcttgc cgggttcgac cccgcgcgtg
       61 acctgctggt gctggtcgac gacgtggccc tccccgccgg gaagttccgc cttcggggcg
      121 ccggctcggc cggaggacac aatggcctaa agagcatcga gggaacgctc cgccggcagg
      181 actacccccg gctccgcatc ggcgtcgggc cggtgcccgc cggcctgttc gaccttgccg
      241 acttcgtgct cgccgctcct tcaccggatg accacgccgc gatcaccgaa ctcctcgagc
      301 ccatgagcca ggcggtcgaa gtctggcttt ccgacgggat cgagaaggcg atgagtttgt
      361 tcaatcggta gtcgttggtc gttggtcgtt cgtcgttcgg cgcaggtccc ccgaacctcg
      421 cgcgagcggc ctccgagcac ccgcaccggc gttccgcaat cggcgttgga gcgccgcgat
      481 ggctcgaccc agcctaccca tgtcggattc cagcgacgct gacagttcga cgtcgagcca
      541 cccgaggtct ctggccagca gcagttcagc ctcaagctcg tggagcgacc cgagcgcgat
      601 gcccgcgaat ctgaccagct ctgcatcgct gcgccgcccc gcgccttcgg cgagattcgc
      661 agagactgag acggccgcac gccgggtttg cgcggtcaac ccgaaccgtt cggcgggtgg
      721 gaatccaccc gtggcccgat agaccgcgag ggccagtcca tgcgcggccc gccatacttt
      781 caagtccttg aaatacccca tcccacctcc tcgccttcga caagttggga tgggaagtca
      841 tcccgtgcgt cacccattcc gtgcatctcc ggtactgaag cccgcgacac tcgactagct
      901 taccccgcgc gaccgactga ccgctgccgt cttgcgaacg accaacgacc atcgaccaac
      961 gactctcaat gctcagactc ggtatcgtcg gccttcccaa cgtcgggaag tcgacattgt
     1021 tcaacgccct cacgcaggcg ggcgcgctgg tggccaacta ccccttcgcc acgatcgagc
     1081 cgaacaccgg cgtggttacg gtgcccgacc cgcggctcga cgaactggcg cggatcgttt
     1141 cgccggagcg caccgtgccc gccgcggtcg agttcgtgga catcgccggc ctggtcaagg
     1201 gcgcctccca gggcgagggg ctcggcaacc agttcctcgc caacatccgc gaggtcgacg
     1261 cgatcgtcca cgtggtgcgc tgctttgccg atgacaacat ccagcacgtg atgggcgcag
     1321 tcgacccggc gcgcgatcgc gacatcatca attccgagct cggcctcgcc gacctggcgg
     1381 tggtcgagaa gcggctcgac aaggcggccc gcgccgccaa gtcgggcgac gccgtggcca
     1441 aggtggaaca cgccctcctc cagcgcctcc accccgccct cgccgacggc aagccggccc
     1501 ggagcgtggt gccgaccgat gaggaagcgc cggtcttccg caccctcaac ctcctcaccg
     1561 ccaagccggt gctctacgcc gccaacgtcc ccgagggcga gatcaacgcc gggaacgcgt
     1621 tcgtccaggc cctcgccgcc gccgtggcga aggacggcga gaccgccgag atcgtcacat
     1681 tctcggccca ggtggagatg gagctcgccg aactggcgcc cgacgagcgg gtccccttcc
     1741 tcgagtcgct gggcctcacc gagtccgggc tcgaccgcct ggcgcgcgcc gcctaccacc
     1801 tcctcggcct gcagagctac ttcaccgccg gcgagaagga ggtccgggcc tggaccatcc
     1861 accggggtga ccgcgctccg gcggccgccg gcgtcatcca ttccgacttc gagaagggct
     1921 tcatccgcgc cgagacggtg agctacgccg atttcgtgcg ggtgggtggg tggaagggcg
     1981 cccgcgacca gggactcgcc cgcgcggaag gcaaggagta cgtggtgcag gatggggatg
     2041 tgatgctgtt ccgattcacg agttagtcgc gactcgcgac cctcgactat atttcccccg
     2101 tccctttcac gagtccctaa tgtccgatcc gaatctctat cgttccgccg cgccgaagta
     2161 tgccggcctc gacgaatccc aggccctgat cgcgtcgttc ctccgccggg tgtacggctg
     2221 gatgttcgtg ggcctcgccg ccaccgccat caccgcctgg caggtcgcga gctccgaggc
     2281 ggcggtcaag gtcatcttcg gcacgccgtt cctcttcttt gccctcctct tcggcgagct
     2341 ggggctcgtg tggtggatct cggcccgcgt gggcaagatg gcgccgagca ccacggcggc
     2401 gctcttcgtg gtgtactcgg tcgtgaacgg cctcaccctg tccgtggtgc tcctcgccta
     2461 caccggtgcc tcgatcgaga cgaccttcct taccgccgcg gcgatgttcg gggcgctggc
     2521 gctcttcggc accctcaccg gccggaacct ccagggcatg ggacagttcg ccatgatggg
     2581 gctcttcggg ctgctcgcag cgatgctcgt cggcttcttc tggcacagcg acgcactgca
     2641 gttcatcatc tcgatcgtgg gcgtgatcgt cttcaccggt ctcaccgcct gggacgccca
     2701 gaagctcaag tcgatggccc tggcgctcga gggccgggag agcggcggct atgcgatctc
     2761 gggtgcgctg gccctctacc tcgacttcgt caacctcttc ctcttcctgc tccggttcct
     2821 cggccgccgc cgttaggcca cgccgaccgc ggacaggaga gtcttccgag cgccgtccca
     2881 cccaccgggc ggcgctcgcc gttccagcac ctttgccgtc gtcaccaccc cggaggcccc
     2941 gcatgcgtcg cccctcgctc gccgtgatcc tcaccgccct gctcgcggtg gccccggcgc
     3001 tcgccgccca gcgcccgacc ggcgtcatcg gcgcctacac tccgccccgc gactg
//
LOCUS       DASUAP010000017         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_627292, whole genome shotgun sequence.
ACCESSION   DASUAP010000017 DASUAP010000000
VERSION     DASUAP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627292"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VFU45_00480"
     CDS             complement(<1..1033)
                     /locus_tag="VFU45_00480"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HEU5357563.1"
                     /translation="MSAARAVPGIVLLLVGAAAAHAQVPPRATIAGCVYDTLASAPVR
                     LATVRVAGTTRSLLTDDAGCFRIDVDTGDVTVDVRQVGYRPAALHLFAWAGINRRNVY
                     LHPFAVQVAPLTITATPDEATRLIRAAIARKHELAATTHDVRYQAYVKFAVRDLSKPP
                     DSAASILVITESRSQIFWEPPDHYQETILSRRQTGNLQAGQNLVTAGEIADFQRERIE
                     FRQFSVVSPIADDALDEYRYALLDTLDVDGRRIFRLALTPRSEAVPLFIGTVDIADST
                     FDLAGIDVGFDRAVHFTALKELRYRQRLREVAPGRWMPYRITLSGELHLTIPLPRFPH
                     DLAFQHVAEL"
     gene            1254..2003
                     /locus_tag="VFU45_00485"
     CDS             1254..2003
                     /locus_tag="VFU45_00485"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEU5357564.1"
                     /translation="MQKRTFIQSLAVLAASSVVRADRLAAWKPRRRKDLAWALNLDVL
                     ETCSCPVFCQCFFTDKPPASMDGPGMSEQHVCRFDQAYRVNAGQVGAVQLDGARFWFA
                     GDAGDDFDKPKLAWAELTFDSSLSREQRDALLVALRHLHWYRPDRWMSYSIADAAPMT
                     WTADARGAHATLGGGQLGEVKLTTMIGQHDRPVMMNNMDYFGYPRNNGFVLMPSDVLS
                     YHRGDRSFEYRGKGTNGLHTTLEMTADDFPA"
BASE COUNT          379 a          796 c          845 g          361 t
ORIGIN      
        1 tgagctccgc cacatgctgg aacgcgaggt cgtgcgggaa gcgtggcagc gggatcgtga
       61 ggtgcagctc gccgctcagc gtgatccggt acggcatcca gcgtcccggc gccacctcgc
      121 ggagccgctg gcggtagcgg agctccttga gcgccgtgaa gtggaccgcg cggtcgaagc
      181 ccacgtcgat ccccgccagg tcgaaggtcg aatcggcgat atcgaccgtg ccgatgaaga
      241 gcggcaccgc ctccgagcgg ggggtcaggg ccaggcggaa gatccggcgg ccatcgacat
      301 cgagcgtgtc aagcagcgca tagcggtact cgtcgagcgc atcgtctgcg atcggcgaga
      361 ccaccgagaa ttgccggaac tcgatccgct cacgctggaa gtcggcgatc tcgccggcgg
      421 tcacgaggtt ctggccggcc tgcaggttcc ccgtctggcg gcgggagagg atcgtctcct
      481 ggtagtggtc gggaggctcc cagaagatct ggctcctgga ttcggtgatc accaggatcg
      541 acgccgccga gtcgggcggc ttcgacaggt cgcgcacggc gaacttgacg tacgcctggt
      601 agcggacgtc gtgggtggtg gcggcgagct cgtgcttccg ggcgatcgcc gcgcggatga
      661 gccgcgtggc ctcgtccggg gtggcggtga tcgtgagtgg cgccacctgc accgcgaaag
      721 ggtggagata gacattgcgg cggttgatgc ccgcccacgc gaagaggtgg agcgccgccg
      781 ggcggtaccc cacctgccgg acgtcgaccg tgacgtcgcc ggtatcgacg tcgatccgga
      841 agcatcccgc gtcgtcggtg aggagcgacc gggtcgtgcc ggccacgcgg accgtggcga
      901 gccgcaccgg cgcgctcgcg agcgtgtcgt agacgcagcc ggcgatcgtg gcgcgcggcg
      961 gcacctgggc gtgcgcggcg gctgcgccga ccaggagcag gacgatcccc gggaccgcgc
     1021 gtgccgcaga catcactctg gaacgtgcca ccctgcctcc cgggccggcg acgctcgcgc
     1081 ggcgcaggcc gaattccctc gacatcggtg caaggtatcc cgggcggcgt ggcgctgcac
     1141 cggcgccctc gagggcgggc gccgcatcca tcttgaccat cccggtaccg ggctgcatag
     1201 tggcgactgg cgccgaaacc gtcgccgacc cacccgacac cctcggaggt cccgtgcaaa
     1261 agcgcacatt catccagagc ctggccgtgc tcgccgcctc gtccgtggtc cgcgcggatc
     1321 gccttgccgc gtggaagccg cgccggcgga aggatctggc gtgggcgctc aatctcgatg
     1381 tcctcgagac gtgcagctgc ccggtgttct gccagtgctt cttcactgac aagccgccag
     1441 cgtcgatgga cggcccggga atgtccgagc agcacgtctg ccggttcgac caggcctacc
     1501 gggtcaatgc cgggcaggtc ggcgcggttc agctcgacgg cgcgcgattc tggttcgcgg
     1561 gcgacgccgg ggacgacttc gacaagccga aacttgcgtg ggccgagctc accttcgact
     1621 cgagcctctc ccgcgagcag agggacgccc tccttgtcgc gctgcggcac ctgcactggt
     1681 accgtcctga ccggtggatg tcctactcga tcgccgatgc ggcgccgatg acctggacgg
     1741 ccgatgcccg gggcgcccac gcgacgctcg gcggcgggca gctgggggag gtcaagctga
     1801 ccacgatgat cggccagcac gaccgcccgg tgatgatgaa caacatggat tacttcggct
     1861 atccacggaa caacggcttc gtgctcatgc cgagcgatgt cctgtcgtac caccggggcg
     1921 accggtcgtt cgagtatcgg gggaagggga cgaacgggct gcacaccacc ctcgagatga
     1981 ccgcggacga cttcccggca tgacctgacc ccggcggcga ccaaggtggg gcgccgccgg
     2041 gcagtcttga cgccaaaaat cttcggtata tattcggtat caaccgaaca cacaccgaag
     2101 cgagccagct atgcacgccc cagccccccg cccgtacccg ccggcccaga ccgaccgcaa
     2161 ccccgagacc ctgctcctga tcaaggtgtg ccccgagtgc gccggacccg tggtgcggtc
     2221 gtcgggatgc gtggcgtgcg tccagtgcgg gtggggaagg tgcgggtgag tacggacgct
     2281 atccgctatc cgttatccga tgctccgagc gccgatcggt cccttggacg cgatcggggt
     2341 ccgatcgctc accggcgcat cggataacgg atagcggata g
//
LOCUS       DASUAP010000018         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_927188, whole genome shotgun sequence.
ACCESSION   DASUAP010000018 DASUAP010000000
VERSION     DASUAP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927188"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VFU45_00490"
     CDS             complement(<1..1458)
                     /locus_tag="VFU45_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEU5357565.1"
                     /translation="MGIVNAGQLAVYDDIEPALLERVEDLVLNRRPDATERLLEVADV
                     AARSSRESGPDLSWRKQPVGERLTHALVEGIADFVVEDTEEARQQAARPLDVIEGPLM
                     DGMNVVGDLFGAGKMFLPQVVKSARVMKRAVAHLLPFMEAEKSADAQAKGRILLATVK
                     GDVHDIGKNIVGVVLQCNNYEVIDLGVMVPAAKILEAAAERNVDIIGLSGLITPSLEE
                     MTFVASEMERTGLDLPLLIGGATTSRAHTAVKIEPAYRRGPTVHVADASRAVGVVGSL
                     LSPEQRADYAVRVRDEYRALREERSSRRAGERRVTIEEARANRLVPDWARPVPVPAFT
                     GVRYLRDYPLAELLPRIDWTPFFQSWEMRGHYPDILNDPVQGKAATQLFDDARTLLAQ
                     LVAGGRLRAAAAFGFWPAAARGDDLVLRLSDGGEAVSRTLRQQMQKGAGRANLALADF
                     VAPEGNGITDYVGLFAVTAGLGAPELIAERRAQHDD"
     gene            complement(1456..3144)
                     /locus_tag="VFU45_00495"
                     /pseudo
     CDS             complement(1456..3144)
                     /locus_tag="VFU45_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
BASE COUNT          442 a         1142 c         1088 g          481 t
ORIGIN      
        1 gtcatcgtgc tgcgcgcgcc gctcggcgat cagctccggc gcacccaggc cggcggtcac
       61 ggcgaagagc cccacatagt cggtgatgcc attgccctcc ggcgccacga agtcggccag
      121 cgccaggttg gcgcgtcccg ctcccttctg catctgctgc cgcagggtgc gcgataccgc
      181 ctcgccgcca tcggagaggc ggagcacgag gtcgtcgccg cgggcggcgg cgggccagaa
      241 accgaaggcc gcagccgccc ggaggcggcc tcccgccacc agctgcgcca gcagcgtccg
      301 ggcgtcgtcg aagagctgcg tcgccgcctt gccctggacc gggtcgttca ggatgtcggg
      361 gtagtgcccg cgcatctccc acgactggaa gaacggcgtc cagtcgatgc gcgggaggag
      421 ctcggcgagc gggtagtccc tcaggtagcg gacaccggtg aacgcgggca ccggcaccgg
      481 ccgcgcccag tccggcacca gccggttggc gcgcgcctcc tcgatcgtga cccggcgctc
      541 gccggcccgg cggctcgagc gctcctcccg gagggcgcgg tactcgtcac gcacgcggac
      601 ggcatagtca gcgcgctgtt ccggactgag caggctcccc accacgccca ccgcgcggga
      661 ggcgtccgcc acatgcaccg tggggccgcg gcggtaggcc ggctcgatct tgaccgcggt
      721 gtgcgcccgc gaggtcgtcg cgccgccgat caggagcggc aggtccagcc cggtgcgctc
      781 catctcgctc gcgacgaagg tcatttcctc gagtgacggc gtgatgagtc cggaaaggcc
      841 gatgatgtcc acgttccgtt cggcggccgc ctccaggatc ttcgcggccg ggaccatgac
      901 gccgagatcg atcacctcgt agttgttgca ctgcaggacc acgccgacga tgttcttgcc
      961 gatgtcgtgc acgtcgccct tgacggtggc gagcaggatc cggcccttcg cctgcgcgtc
     1021 ggcggacttc tccgcttcca tgaacggcag caggtgcgcc accgcccgct tcatcacccg
     1081 ggcgctcttc accacctgcg gcaggaacat cttcccggcg ccgaacaggt cgccgacgac
     1141 attcatgccg tccatgagcg ggccctcgat cacgtcgagc ggccgggcgg cctgctggcg
     1201 cgcctcctca gtgtcctcca cgacaaaatc cgcgattccc tccaccagcg catgcgtgag
     1261 ccgttcaccc accggctgct tccgccagga gaggtccggg ccgctctcgc ggctgctccg
     1321 ggccgccacg tcggccacct ccagcagccg ctcggtggcg tcggggcgcc ggttgaggac
     1381 caggtcctcc acccgctcca gcagggcggg ctcgatgtca tcgtacacgg cgagctggcc
     1441 ggcgttgaca atgcccatgt ccaggccggc ggcgatggca tggtagagga agacggcgtg
     1501 gatcgcctcg cgcaccggat cgttcccgcg gaacgagaac gagacgttgc tgatgccgcc
     1561 gctcaccagc acgcgcggaa gctgctcctt gatgcggcgg gtcgcctcga tataggcgac
     1621 cgcgtatccc gcgtgctcct cgatgccggt accgatcgcg aagacgttgg ggtcgaagat
     1681 gatgtcctcg ggcgggaagc cggcctgctc ggtcagcagg cgataggccc gtgcacagac
     1741 cgcgacccgc cgctccaccg tgtccgcctg gccctcctcg tcgaaggcca tcaccaccac
     1801 cgccgcgccg gagcggcgcg ccagctgcgc atgctccagg aacggcccct cgccctcctt
     1861 gagctagacg gagttcacca cgcccttccc ctggacgcag cggagcccgg cctcgatcac
     1921 cgaccacttc gaggagtcga tcatcaccgg cacgcgggag atggcgggct ccgaggccac
     1981 gagattgagg aaggtcgcca tggcccgttc ggcatccagc atgccctcgt ccatgttcac
     2041 gtcgatcatc tgcgcgccgt tttccacctg ctggcgcgcc acctcgagcg ccgcctcgtg
     2101 ctccccgccg aggatgagct tcgcaaagcg ccgggacccg gtgacgttgg tgcgctcgcc
     2161 cacgttcacg aagagtgagt cgtccttgat gatcagcggc tccagtccgg ccagccgggt
     2221 ggccggcggt accgcgggcc gttgccgggg cgtcaggccg cgcacggcgg acgcgatggc
     2281 agcgatgtgc tccggcgtgg tgccgcagca gccgcccacg atgttcacca gtccgtgctc
     2341 cgcccactcc cggagctggg tggacattgt ttccggcgtc tcgtcgtagc cgccgaactc
     2401 gttcgggagg ccggcgttgg ggtgcgcgct cacgtacacc ggcgccacgc gaccgacatc
     2461 ctgcacgtac tggcgcagct gcttcgcgcc gagggcacag ttgaggccga tgctgagcgg
     2521 cgccgcgtgc gccacgctgt accagaatgc ttctgcggtc tggcccgaga gcgtccggcc
     2581 gctggcgtcg gtgatggtgc cggagatcat caccggcagc cgcggtccac cccgggcgac
     2641 gaggctctcg agcgcgaaga tcgcggcctt cgcgttgagc gtgtcgaaga cggtctcgat
     2701 cagcagcagg tcggcgccgc cttccaccag cgcggcggcg gcctcctcgt acgcctcggc
     2761 cagggccgcg aacgacgtgt tccgcgcgcc gggatcgttc acgtccggcg agatgctggc
     2821 ggtgcggttg gtgggaccaa gcactcccgc cacgtaccgg gggcgggagg ggtcggccgc
     2881 ctcgaattcg tcggccacgg cgcgcgccag gcgggcgccc tcgcgattca actcggcaac
     2941 gagcgactcc gcgccgtaat ctgcctgggc gatccgggtc gcgttgaacg tattggtctc
     3001 gatgatgtcg gcgccggcgg cgaggtactg ccggtgcaca tccgccacga tggccggctg
     3061 ggtgagcagc agcaggtcgt tgtcgccctt gaggtcccgc ggccaggcgg cgaaccgctc
     3121 gccccggaag gcggcctcgt ccaggcggtg ccg
//
LOCUS       DASUAP010000019         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_177547, whole genome shotgun sequence.
ACCESSION   DASUAP010000019 DASUAP010000000
VERSION     DASUAP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177547"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFU45_00500"
     CDS             <1..471
                     /locus_tag="VFU45_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5357566.1"
                     /translation="SDTHNLLRPEVFEVFREVDLILHAGDIGDPGLLTELEAIAPVIA
                     VWGNTDGMDIRARVPEVVQRRIEGIDFVLVHGHQVGSPTPEKLHAAYPEAEVIVYGHT
                     HRPLLVNLEVVVTVMNPGGAGARRFDLPPSVGIMELEAGLPPRARLVPLQDLDE"
     gene            500..1612
                     /locus_tag="VFU45_00505"
     CDS             500..1612
                     /locus_tag="VFU45_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5357567.1"
                     /translation="MRRLIFLLFLPVALSAQARPRARALGIAPGFLPPGRWNAITDVA
                     GVRVGQVTIDSGDSIHTGVTAIIPEDSNTFAWRLPAAIYVGNGFGKLLGVTQVQELGE
                     IESPILLTCTLCVWRAADALTGWMLARPGMGPVRSFNPVVGETNDGGLSAIRLRPIRP
                     EDVIRALEGAASGPVEEGSVGAGRGTINFGWKGGIGTSSRKLPDRLGGWTVGVLVQTN
                     YGGDLTVMGVPVGRIWGHQPFPEPRGPGGSVMVVVATDAPLLERNLGRLAKRATFGLA
                     RTGSWMDNGSGDYVIAFSTAPDLRRPFGATRVGGLEVSNDQMSPLFAAIIEATEEAVY
                     NALLAATPVRNAQGRTADSLPGDRLQALLRERHAIP"
     gene            complement(1777..2880)
                     /locus_tag="VFU45_00510"
     CDS             complement(1777..2880)
                     /locus_tag="VFU45_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEU5357568.1"
                     /translation="MKQLSALALPATDLDALRARVKALAEPRPGVYRMVDPAGRVLYV
                     GKARRLRARLLSYFRARYPDDKAARILHSAHDIQWDYAPSEFAALLGEIREIRRHRPP
                     YNFQGNRSRLPVFISVTSDLAPRLEARRRAPTTEERCYGPFGSAGRVEEGLRTLNDLL
                     GLRDCAAKMPIVFADQTDLFAAPRQAACLRHQFGSCLGPCAALTTREAYDGRMEDAIA
                     FLEGRSLAPVGRVVELMQQAAASQSFEEAARWRERFEALEWLLSATTRARAAVELLSF
                     VYRDPGIHGDDRAYLIKRGTVRAAFPYPTTPIEIEAFRAVVAQELARPEPPAGLLPVA
                     RLDEMLVVMSWFRRHPESFRRTTKLEEWSSVPN"
     gene            complement(2877..>3679)
                     /gene="mutM"
                     /locus_tag="VFU45_00515"
     CDS             complement(2877..>3679)
                     /gene="mutM"
                     /locus_tag="VFU45_00515"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU5357569.1"
                     /translation="TIVRDLRPALVGRRIEQAALSHEDVLRGVTRRRLVTSLPRRTIH
                     DLSRRAKHAIFDLGDRRLVVQPGMTGALLTYDHALTAAERRYAVLRLTLDDGRLLVYR
                     DVRRLGTLLLLDARGWAAYDAALGPEPLADDFTPEVLHRSFARSRQAVKKVLMDQRHV
                     VGVGNIYANEALFAAGIDPSRPAAKLSLADATRLHTEVRRILRAAIASQGTTFRDYRT
                     GTGERGNFQLELQVYGREGEPCRVCGTRLAGTHAIDQRITVFCHRCQA"
BASE COUNT          528 a         1278 c         1339 g          534 t
ORIGIN      
        1 tccgacaccc acaacctcct gcgtccggag gtcttcgagg tcttccgcga ggtggacctc
       61 atcctccacg cgggcgacat cggcgacccc ggccttctca ccgaactcga ggcgatcgcc
      121 ccggtcatcg ccgtctgggg gaacaccgac gggatggaca tccgcgcccg cgttcccgag
      181 gtggtccagc ggcggatcga ggggatcgac ttcgtgctgg tgcacggcca ccaggtcgga
      241 agcccgacgc cggagaagct gcacgccgcg tacccggagg ccgaggtcat cgtctacggc
      301 cacacccatc ggccgctgct cgtgaacctc gaggtcgtgg tgacggtcat gaaccccggc
      361 ggggccggcg cccggcggtt cgacctgccg ccatcggtcg gcatcatgga acttgaggca
      421 ggattgccgc cgcgcgctcg gctggtgccg ctgcaggatc tcgacgaatg actggtgcct
      481 tgtcccgatc ggaggatcta tgcgtcgact gatcttcctc ctcttccttc ccgtggcgct
      541 gagcgcgcag gcccgcccgc gcgcccgcgc cctcggcatc gcgccggggt tcctcccgcc
      601 gggccgctgg aacgccatca ccgacgtcgc gggcgtccgg gtggggcagg tcaccatcga
      661 ctcaggtgac tcgatccaca ccggcgtcac cgccatcatc ccggaggact ccaacacgtt
      721 cgcctggcga ctccccgccg ccatctacgt cgggaacgga ttcgggaagc tcctcggcgt
      781 cacgcaggta caggagctcg gcgagatcga gtcccccatc ctcctcacct gcaccctctg
      841 cgtctggcgg gcggcagacg ccctcaccgg ttggatgctc gcccggccgg gaatggggcc
      901 ggtgcggtcg ttcaacccgg tggtcggcga gacgaacgat ggggggttga gcgcgatccg
      961 gctccggccg atccggccgg aggacgtgat ccgcgcactg gagggtgccg cgagcgggcc
     1021 ggtcgaggag gggagcgtcg gtgcggggcg ggggacgatc aacttcgggt ggaagggcgg
     1081 gatcggcacc agcagccgca agctcccgga ccggctcggc ggatggaccg tgggcgtcct
     1141 ggtgcagacc aactatggcg gcgacctcac ggtcatgggc gtgcccgtcg ggcggatctg
     1201 ggggcaccag ccgttcccgg aaccccgcgg ccccggcggc tcggtgatgg tcgtcgtggc
     1261 gaccgacgcc ccgctcctcg agcggaacct tgggcgcctc gccaagcgcg ccacgttcgg
     1321 cctggcgcgg acggggtcat ggatggacaa cgggtcgggc gattatgtga tcgcgttctc
     1381 gacggcgccc gaccttcgac gccctttcgg cgccacccgc gtaggcggcc tcgaagtgag
     1441 caacgaccag atgagcccac tcttcgccgc catcatcgag gccaccgaag aggccgtcta
     1501 caacgcgctt ctggcggcga cgccggtcag gaacgcgcaa ggtcgaacgg ccgactctct
     1561 gcccggcgac cggctgcagg ccctgcttcg cgagcgccac gcgatcccat gaggtggcgc
     1621 cgacccctcg ccgtcacttc gcgtcgaccg ccggcggact gcgcatgaag ggccgaggcg
     1681 gcgggcgagg cgagcaggac ggcggcaagg catcgggtgg gcatcgcggt tcctggagtt
     1741 ggacagccac gatgcgtcag cgccgcgatg actctgtcaa ttcggaactg aagaccactc
     1801 ttccagcttg gtcgtccgcc ggaacgattc cgggtgtcgg cggaaccagc tcatcaccac
     1861 cagcatctcg tcgagcctgg ccaccggcag gagtcccgcc ggcggctcgg gccgcgccag
     1921 ctcctgggcc accacggcgc ggaacgcctc gatctcgatg ggtgtcgtgg gatacgggaa
     1981 cgccgcccgc acggtgccgc gcttgatgag gtacgcccgg tcgtcgccgt ggatgccggg
     2041 gtcgcgatag acgaagctca ggagttccac cgccgcgcgg gcgcgcgtcg tggccgagag
     2101 gagccactcc agcgcctcga accgctcgcg ccaccgcgcc gcctcctcga acgactggct
     2161 cgccgccgcc tgctgcatca gctccaccac ccggcccacc ggcgccagcg acctcccctc
     2221 caggaaggcg atggcgtcct ccatcctccc gtcgtacgcc tcgcgggtgg tcagcgccgc
     2281 gcagggaccc aggcaggagc cgaactggtg gcggaggcag gcggcctggc gcggcgcggc
     2341 gaagaggtcg gtctggtcgg caaagacgat gggcatcttg gcggcgcagt cccggaggcc
     2401 aaggaggtcg ttgagcgtgc ggagcccttc ctccacccgc cccgccgagc cgaagggtcc
     2461 ataacagcgc tcctcggtgg tcggcgcacg tcggcgcgct tcgagccggg gcgccaggtc
     2521 gctggtgacg ctgatgaaca ccggcaagcg ggagcggttg ccctggaaat tgtagggcgg
     2581 gcggtgccgt cggatctcac ggatctcccc caatagcgcc gcgaactcgc tcggcgcgta
     2641 gtcccactgg atgtcgtgcg ccgagtggag gatccgcgcc gccttgtcgt cgggatagcg
     2701 ggcgcggaaa tagctgagga gccgcgcgcg gagtcggcgc gctttcccga cgtagagcac
     2761 gcgtcccgcc gggtccacca tccggtagac acccggtcgc ggctccgcca gcgccttgac
     2821 gcgcgcgcgc agcgcatcga ggtccgtggc cgggagggcg agggcgctca gctgcttcat
     2881 gcctggcacc ggtggcagaa cacggtgatc cgctggtcga tcgcgtgggt cccggccagg
     2941 cgggtgccgc acacccggca cggttctcct tcacgcccgt agacctgcag ttcgagctgg
     3001 aagttgcccc gctcgccggt gccggtgcgg taatcgcgga aggtggtgcc ctgggaggcg
     3061 atggcggccc ggaggatccg gcgcacctcc gtgtgcagcc gggtggcgtc ggcgagcgac
     3121 agctttgctg ccggtcgcga cgggtcgatc cccgccgcga agagcgcttc gttggcgtag
     3181 atgttcccca cccccaccac gtggcgctgg tccatcagca ccttcttcac ggcctggcgc
     3241 gaccgcgcga agctccggtg cagcacctcc ggcgtgaagt cgtcggcgag cggctccggg
     3301 ccgagcgccg cgtcgtaggc cgcccacccg cgcgcatcga gcagcaggag ggtgccgagc
     3361 cgccggacgt cgcggtagac gaggagccgg ccgtcatcga gcgtgaggcg gaggacggcg
     3421 taccggcgtt cggcggcggt gagcgcgtgg tcgtaggtaa ggagagcgcc ggtcatgccc
     3481 ggttggacca cgaggcggcg gtcgcccagg tcgaagatcg cgtgcttggc gcgacgggag
     3541 aggtcgtgga tggtgcgtcg gggcagggag gtcacgaggc gacggcgggt gacgccccgg
     3601 aggacgtcct cgtgtgagag ggccgcctgc tcgatccgtc ggcccaccag tgcggggcgc
     3661 aggtcccgga cgatcgtct
//
LOCUS       DASUAP010000020         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_627379, whole genome shotgun sequence.
ACCESSION   DASUAP010000020 DASUAP010000000
VERSION     DASUAP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627379"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="VFU45_00520"
     CDS             <1..1421
                     /locus_tag="VFU45_00520"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5357570.1"
                     /translation="DLSFSEDVQKRFTGYGWKVIRVSSGTDLKAIQRALAAARRETDR
                     PTLIILRTIIGEPAPNKQNTSGAHGAPLGAAEVKATKQMMGWPETPFYVPAEVDAQRA
                     RFARRGRREGRAWRRLFDAYAAQHPAAAAEFEAWMRGTLPAGWDAALPDFAGQSGLAT
                     RQASQSVLEAAAKVLPNLVGGSADLAHSTGTVLKEGGEFTAKAPGRNVHWGIREHGMT
                     AALNGMAAHGGVRPFGSTFLIFSDYLKPSLRLAALMKLPVIVIGTHDSIGVGEDGPTH
                     QPVEQLAALRATPNVHVFRPADATETTECWRMALERTDGPSVLALTRQKVPVLDRTKL
                     RPAAGARRGGYILFEPPTAPSAIILGTGSEVQLALAAAERLTAAGTPTRVVSLPSWEV
                     FEAQPASYRDQVLPPAVTARVSVEAASTFGWARYTGTAGVAVGLDHFGASAPAETLFQ
                     EFGITADRVVDAVRRSLAQGAA"
     gene            1421..>2446
                     /gene="tal"
                     /locus_tag="VFU45_00525"
     CDS             1421..>2446
                     /gene="tal"
                     /locus_tag="VFU45_00525"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU5357571.1"
                     /translation="MSNPLVTLGKLGQSPWYDFITRDLVQSGKLRRLIDDDGLLGMTS
                     NPTIFEKAVSSSDLYDEDIRRLTAEGKNAAEVFESLAVADVQSACDVFSETYERTRGM
                     DGLVSLEVSPTLARNTVGTIAEAERLWKRVARPNVMIKIPGTAEGLEAITRCLAAGIN
                     VNVTLLFSVARYREVIDAFFRGIEQRAAVGEPVDRVASVASFFVSRVDGRVDPELDKK
                     GDPSKLRGTIAIANAAAAYAIFEEEFQGPRWEKLVARGAQVQRPLWASTSTKDPKYPD
                     TYYVDALIGPHTVNTLPPETFAAFGDHGKPAVRIREAMARAPGRLEALAGCGIDLGAV
                     TTFLENDG"
BASE COUNT          360 a          900 c          866 g          320 t
ORIGIN      
        1 ccgacctctc gttctccgag gacgtgcaga agcgcttcac cggctacggc tggaaggtca
       61 tccgcgtctc gagcggaacg gacctcaagg ccatccagcg cgcgctcgcg gccgcgcgcc
      121 gcgagaccga ccgccccacg ctcatcatcc tccgcaccat catcggcgag cctgcgccca
      181 acaagcagaa cacctccggt gcccacggcg ccccgctcgg cgcggcggag gtgaaggcca
      241 ccaagcagat gatggggtgg ccggaaacgc cgttctatgt gcccgccgag gtcgatgccc
      301 aacgggcgcg gttcgcgcgg cggggacgcc gggagggccg ggcgtggcgg cgcctgttcg
      361 acgcgtacgc cgcccagcac ccggcggcgg ccgcggagtt cgaggcctgg atgcggggca
      421 cgctgccggc gggctgggac gcggccctcc cggacttcgc gggccagtcg gggctcgcca
      481 cccgacaggc gtcgcagtcg gtgctcgaag ccgccgccaa ggtgctgccc aacctcgtgg
      541 gtgggtccgc cgacctggcc cactcgaccg gcaccgtcct caaggagggc ggcgagttca
      601 ccgccaaggc gccgggccgc aacgtgcact ggggcatccg cgagcacggg atgacggcgg
      661 cgctcaacgg gatggcggcg cacggtgggg tgcggccctt cggcagcacg ttcctcatct
      721 tctcggacta cctcaagccc tccctgcggc tggccgcgct gatgaagctg ccggtcatcg
      781 tgatcggtac ccacgactcg atcggcgtgg gcgaggacgg gcccacccac cagcccgtcg
      841 agcagctggc ggccctccgc gccacgccga acgtccacgt gttccgcccg gccgacgcca
      901 cggagacgac cgagtgctgg cggatggcgc tcgagcggac cgacgggccg agcgtcctgg
      961 cgctgacgcg ccagaaggtg ccggtcctcg accgcacgaa gctccggccc gccgccggcg
     1021 cacgccgtgg cgggtacatc ctcttcgagc cgccgacggc gcccagcgcc atcatcctcg
     1081 gcaccggctc cgaggtgcag ctggcgctcg ccgcggccga gcggctgacc gcggccggca
     1141 cgccgacgcg ggtggtgtcg ctcccgtcgt gggaggtgtt cgaggcgcag ccggcgagct
     1201 accgcgacca ggtgctcccc ccggcggtca cggcgcgggt gtcggtggag gcggcgagca
     1261 ccttcggatg ggcccgctac accggcaccg ccggggtggc cgtggggctc gaccacttcg
     1321 gcgcctcggc tccggccgag accctgttcc aggaattcgg gatcaccgcg gaccgcgtcg
     1381 tcgacgccgt ccggcgatcg ctcgcgcagg gggccgcctg atgtccaacc cgctcgtcac
     1441 cctcgggaag ctcggccaga gcccgtggta tgacttcatc acccgcgacc tcgtgcagtc
     1501 gggcaagctc cggcggctca tcgatgacga tggcctgctg ggcatgacgt ccaacccgac
     1561 catcttcgag aaggcggtga gctcgagcga cctctacgac gaggacatcc gccggctcac
     1621 ggcagaagga aagaacgccg ctgaggtctt cgagtcgctg gccgtcgctg acgtccagtc
     1681 cgcctgcgac gtgttctcgg agacctatga gcggacccgg ggtatggacg gcctcgtgtc
     1741 gctcgaagtc tcgcccacgc tggcgcggaa caccgtcggc accatcgcgg aggcggagcg
     1801 gctctggaag cgggtggcgc gccccaacgt catgatcaag atccccggca ccgccgaggg
     1861 gctcgaggcg atcacccgct gcctggcggc ggggatcaac gtcaacgtga cgctgctctt
     1921 ctcggtggcg cgctaccgcg aggtgatcga cgccttcttc cgcggcatcg aacagcgcgc
     1981 cgccgtgggc gaacccgtcg accgcgtggc ctcggtggcg agcttcttcg tgagccgggt
     2041 ggacggccgg gtggacccgg agctcgacaa gaagggcgac ccgtcgaagc tgcggggcac
     2101 gatcgcgatc gccaacgcgg ccgccgcata cgcgatcttc gaggaggagt tccaggggcc
     2161 ccgctgggaa aagctcgtcg cccgcggcgc gcaggtgcag cgccccctct gggcctcgac
     2221 cagcaccaag gacccgaagt atccggacac ctactacgtc gacgcactga tcggcccgca
     2281 caccgtcaac acgctgcccc ccgagacctt cgccgccttc ggcgaccacg ggaagccggc
     2341 cgtccgcatc cgggaggcga tggccagggc ccccggccgg ctcgaggcgc tcgccgggtg
     2401 cgggatcgac ctgggcgccg tcaccacctt cctcgagaac gacggg
//
LOCUS       DASUAP010000021         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_954469, whole genome shotgun sequence.
ACCESSION   DASUAP010000021 DASUAP010000000
VERSION     DASUAP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954469"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1809
                     /locus_tag="VFU45_00530"
     CDS             <1..1809
                     /locus_tag="VFU45_00530"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5357572.1"
                     /translation="ENNVDWALSRDRYWGTPLPVWQCDRDAAHVEVVGSYAQLFERSG
                     KPRPKDFDPHKPFIDAYTWACTCGGRMTRAPEVIDTWFDSGAMPYAQWHQPFEHEAEF
                     RAHFPADYICEGVDQTRGWFYSLLAIATTAFDAPAYRNVIVNELVLDADGQKMSKSRG
                     NVVDPWSVIGEFGADAVRLFLLAGSQVWLPKRFDRRAIPDVAGGFLNTLRNTYGFFQL
                     YAGDWRPGAAAARTRADDWILGRLDRTVAAVRGSWDGYDATQGVRAIMAFVVDDLSNW
                     YVRTNRDRFWAPDASADPAALATLHECLVTVARLLAPAAPFAADWLHRALTANSAHLA
                     TFPAHPGDRLRGAPGLDGAMDAVRRLTSLARAARDAAGLRVRQPLARMTVAVPAEVLG
                     AEFEALLPLLAREVNVKSVEVAASEDSLVRLKVKGNFRALGKRYGKGTPQAVKALEAL
                     TPAQVRDIESGRGAAVTVDGTTFAYGAEDVVVEREVTSDWLVQSAGAFVAALDPALTP
                     ALAAEGLARELVSRVQRLRRDAGFDYTDRIALAVSGAEPVLAAARDHEAFLRSETLAR
                     QLDVGVRLERPDLEQVVDLDGHAVTVGLRRQSGAAA"
     gene            1834..2217
                     /locus_tag="VFU45_00535"
     CDS             1834..2217
                     /locus_tag="VFU45_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEU5357573.1"
                     /translation="MNKKQLAHMEKRLLEERARVMKELGTYDESFNATLQNEDGDLSS
                     YSFHMADQGTDAMEREKQFLMASKEGRYLWHVNQALRRLYNAPEKFGQCETCGAEIQF
                     ERLDALPHARLCINCKEKEEDAKRR"
     gene            2201..2728
                     /gene="lspA"
                     /locus_tag="VFU45_00540"
     CDS             2201..2728
                     /gene="lspA"
                     /locus_tag="VFU45_00540"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU5357574.1"
                     /translation="MPSAAERRLFLGVAAAVALADLVAKLGAEAWLRASAGVPVVGDW
                     VQLRLVFNPGAAFGVSVGSWSRVGFSMVAIGAIVLLARVSRRAEVGDRLRQLACGLVA
                     GGAAGNLIDRIRSAQGVVDFLDVGVGPHRWPTFNVADIGVTTGALVLAWSLWDEDRRA
                     ALAEAVATPEQGALP"
     gene            2737..>3588
                     /locus_tag="VFU45_00545"
     CDS             2737..>3588
                     /locus_tag="VFU45_00545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5357575.1"
                     /translation="MTDIRRFSVTTAASDRLDRFLADQLDLSRTQAARLVAQGAVTVN
                     GAASRASRLLARGDAVEVAWPEAEPPRTLRPAAIPLTIVFEDEHLAVIDKPAGLVVHP
                     APGHWDDTLVNALVARGTTLSGGAAGRPGIVHRLDRDTSGLMVVAKTDLAHRKLGAAL
                     AARRIVRHYAALAWGHLDESPTVIDAPIARNPKDRTRMSVRPEGRAARTDAWVIARFG
                     ETDLLRLELHSGRTHQIRVHLDHIGHPIVGDPVYAGGGSRRIGGPARQRAEAVERATP
                     RQALHAAF"
BASE COUNT          480 a         1297 c         1325 g          486 t
ORIGIN      
        1 gagaacaacg tcgactgggc gctctcccgc gaccgctact ggggcacccc gctcccggtc
       61 tggcagtgcg accgcgacgc ggcccacgtc gaggtggtcg gaagctacgc ccagctcttc
      121 gagcgttcgg ggaagccgag gccgaaggac ttcgacccgc acaagccgtt catcgacgcg
      181 tacacctggg cctgcacgtg cggcggccgg atgacccgtg cgcctgaggt gatcgacacc
      241 tggttcgact cgggggccat gccctacgcg cagtggcacc agcccttcga gcacgaggcc
      301 gagttccggg cgcacttccc ggccgactat atctgtgagg gcgtcgacca gacccgcggg
      361 tggttctact cgctgctggc catcgccacg acggccttcg acgcgccggc ctaccggaac
      421 gtgatcgtca acgagctggt gctcgacgcg gacggccaga agatgtccaa gagccgcggc
      481 aacgtcgtcg acccctggtc ggtcatcggg gagttcggcg ccgatgcggt ccggctcttc
      541 ctcctggccg ggagccaggt gtggctgccc aagcgcttcg accggcgcgc catccccgac
      601 gtggccggcg ggttcctcaa caccctgcgc aacacgtacg gcttcttcca gctgtacgcc
      661 ggcgactggc ggcccggcgc ggccgcggcc cggacccgcg ccgacgactg gatcctgggc
      721 cggctcgacc gcacggtcgc ggcggtgcgg gggagctggg acgggtacga tgccacccag
      781 ggcgtgcgcg ctatcatggc gttcgtggtg gacgacctct ccaactggta tgtccggacc
      841 aaccgcgacc gcttctgggc gccggacgcc tcggccgatc cggcggcgct cgcgacgctc
      901 catgagtgcc tggtgacggt ggcccgcctg ctggcgccgg cggcgccctt cgccgccgac
      961 tggctccacc gcgcgctcac ggcaaattcg gcccaccttg ctacctttcc cgcccatccc
     1021 ggcgaccgcc tgcggggcgc gccggggctc gacggggcga tggacgccgt ccgccggctc
     1081 acctcgctgg cccgtgcggc ccgcgacgcg gcgggactcc gggtccgcca gccgctcgca
     1141 cggatgacgg tggcggtccc cgccgaggtg ctcggggcgg agttcgaggc gctgctgccg
     1201 ctcctcgccc gcgaggtcaa cgtgaagtcg gtcgaggtcg cggcctcgga ggattccctc
     1261 gtccgcctca aggtgaaggg caacttccgg gcactcggca agcgctacgg gaagggaacg
     1321 ccgcaggcgg tgaaggcgct cgaggcgctc acgccggcgc aggtccgcga catcgaatcg
     1381 gggcggggcg ccgcggtgac ggtcgacggg accaccttcg cgtacggcgc ggaggacgtg
     1441 gtggtggagc gcgaggtgac gagcgactgg ctggtgcaga gcgccggcgc ctttgtggcc
     1501 gcgctcgacc cggcgctcac cccggcgctc gcggcggagg gcctggcgcg ggagctggtg
     1561 agccgggtgc agcgactccg gcgggacgcc gggttcgact acaccgaccg gatcgcgctg
     1621 gcggtgagcg gggccgagcc ggtgctggcg gcggcgcggg accacgaggc gttcctccgc
     1681 tcggagacgc tcgcccgaca gctggacgtg ggcgtgcggc tcgagcggcc ggacctggaa
     1741 caggtcgtcg acctcgacgg ccacgcagtg acggtcggac ttcggcgcca gagcggcgcg
     1801 gcggcctgac accacccgaa tccctgggac cctatgaaca agaagcagct cgcccacatg
     1861 gagaagcgcc tcctcgagga acgcgcgcgc gtgatgaagg aactcggcac ctacgacgag
     1921 tccttcaacg cgaccctcca gaacgaggac ggcgacctct cctcctactc gttccatatg
     1981 gccgatcagg gcacggacgc gatggagcgg gagaagcagt tcctgatggc ctccaaggaa
     2041 gggcgctacc tctggcacgt gaaccaggcg ctccgccggc tctacaacgc cccggagaag
     2101 ttcggccagt gcgagacctg cggcgccgag atccagttcg agcgcctcga tgcgctgccg
     2161 cacgcgcggc tgtgcatcaa ctgcaaggag aaggaagagg atgccaagcg ccgctgagcg
     2221 gcggctcttc cttggcgtgg cggccgcggt cgcgctcgcc gacctcgtgg ccaagctcgg
     2281 cgccgaggcg tggctccggg cctcagcggg cgtgccggtg gtgggggact gggtgcagct
     2341 ccggctggtg ttcaaccccg gcgccgcgtt cggcgtgagc gtcgggagct ggagccgggt
     2401 cgggttctcg atggtcgcga tcggcgccat cgtgctcctg gcgcgcgtct cccggcgggc
     2461 cgaggtcggt gaccggctcc gccagctcgc ctgcggactc gtggccgggg gcgcggcggg
     2521 gaacctgatc gaccggatcc ggagcgcgca gggcgtcgtc gacttcctcg acgtgggtgt
     2581 cggtccccat cgctggccga ccttcaacgt ggccgacatc ggcgtcacca ccggggcgct
     2641 ggtgctcgcc tggtcgctgt gggacgagga ccgccgcgcg gcgctcgccg aggcggtcgc
     2701 gacgccggag cagggcgcgc tcccctgacc cctcctgtga cggacatccg ccggttcagc
     2761 gtcaccaccg cggcgagcga ccggctcgac cgcttcttgg ccgaccagct cgacctctcc
     2821 cggacccagg cggcgcggct ggtggcgcag ggggccgtga ccgtcaacgg cgccgccagc
     2881 cgcgcctcga ggctcctcgc ccgcggcgat gcggtggagg tcgcctggcc cgaggccgag
     2941 ccgccgcgca cgctgcgccc ggcggcgatc ccgctcacca tcgtctttga ggacgagcac
     3001 ctcgcggtca tcgacaagcc cgcgggcctg gtggtgcatc cggcccccgg ccactgggac
     3061 gatacgctgg tcaacgccct cgtggcgcgg ggcaccacgc tctccggcgg cgcggcgggg
     3121 cggccgggga tcgtccaccg cctcgaccgg gacacctcgg ggctcatggt ggtggccaag
     3181 accgacctgg cccaccggaa gctgggcgcg gcgctcgccg cccggcgcat cgtccgtcac
     3241 tatgcggcgc tcgcctgggg ccacctggac gagagcccga cggtcatcga cgcccctatc
     3301 gcgcggaacc ccaaggaccg cacccggatg tcggtgcgtc ccgagggccg ggcggcgcgc
     3361 accgacgcct gggtcatcgc ccgcttcggc gagaccgacc tcctccggct ggagctccac
     3421 agcgggcgca cccaccagat ccgggtgcac ctcgatcaca tcggccatcc catcgtgggc
     3481 gatccggtgt atgcgggggg cggaagccgg cggatcggcg ggccggcccg ccagcgcgcc
     3541 gaggcggtcg agcgggcgac gccgcggcag gcgctgcacg cggcgttc
//
LOCUS       DASUAP010000022         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_368360, whole genome shotgun sequence.
ACCESSION   DASUAP010000022 DASUAP010000000
VERSION     DASUAP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368360"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VFU45_00550"
     CDS             <1..784
                     /locus_tag="VFU45_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357576.1"
                     /translation="GVPAPDDDSTALHLANNLRAETRMDGSVRGEFLTTAWRAIELMD
                     LDHGADQAGTVRVIGYLLARQDEPGAYGGRNARPEHAGRSLPGFFSPGDPQRPIAPIT
                     LPTGATIGDDADARYAVSCLALRAALKARLEKRPQVHQHTESLAALAQDWGGPMPLTL
                     ACSTLHGLALAAQPMRDAIPGLIARIAAAESGGVWPDADLFHVLQALLSVPDAAATAL
                     IRRAVPALVTLQRPDGGFDGEEPYGEERGWIATRAFLRARGA"
     gene            complement(945..1661)
                     /locus_tag="VFU45_00555"
     CDS             complement(945..1661)
                     /locus_tag="VFU45_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357577.1"
                     /translation="MGTWVTLGTAIVLGFLHALEVDHMLAVTAFVSRRPALATAAGFG
                     ARWGLGHSISVCLAGGVLLAIGLHWPARYDSLGEIVVGLLLVGLGLWALRSASKLHLH
                     APEGHGDHAHLHHHGHAGAGHAHPHPAPAASHSHDHRHGITWVGMLHGLAGTAGVVAL
                     VPVTLIASRLVGLGYLVAFGVGVTAGMTLFAFVAAAAMRAAAGRSLAWGRRIASAVGL
                     AGIIVGAWWIWRAAGVLASR"
     gene            1689..2750
                     /gene="selD"
                     /locus_tag="VFU45_00560"
     CDS             1689..2750
                     /gene="selD"
                     /locus_tag="VFU45_00560"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEU5357578.1"
                     /translation="MTDAAPAPIRLTAFSHGAGCACKLGATELTEVLRHLPPISDPRI
                     LVDAATRDDAAVYRLDADRALIATTDFFTPIVDDAFDWGRIAAANALSDVYAMGGRPL
                     FALNLVGWPRTSLPFTLLGEVLRGAGQVAAAAGCLLVGGHSVDDPEPKFGLAVVGEVH
                     PDRILTNAAGQAGDLLILTKPLGTGILTTALKRDRVGPEALAPAVESMATLNDGAMRA
                     ALAHGVRCATDITGFGLLGHLGNVLAGSGLGAEVWVDALPLLPGARELAEAGVIPGGT
                     ERNYKAAAGTRWAQDLSRAEQVLCCDAQTSGGLLLAVRPERAEALVAALTAERTPAAA
                     VIGRLVDGPAGSVSVERQR"
     gene            2924..3142
                     /locus_tag="VFU45_00565"
     CDS             2924..3142
                     /locus_tag="VFU45_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357579.1"
                     /translation="MRWTAERMDQLERAVRDGLRVALSRRGSEFIVVARRITSVAQRD
                     ALVGRLPMTNEEKTFILDELDGFSILGA"
     gene            3174..3941
                     /locus_tag="VFU45_00570"
     CDS             3174..3941
                     /locus_tag="VFU45_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5357580.1"
                     /translation="MFTRLLVGLDGSKPSRVALAQAIELGKRFRARLLLAHVSRPAEV
                     AATQGPGRDTGAFTLAILDEAAAAAAEAGLQGEMIQRRGEVVEQLMALAHEADAVFVG
                     RAGLDARDPLGPETRALIQRCPVPVFVAATSVAQFDRCAVAYDGGATSQRALAFAARF
                     VGIRGGRLEVVTAGEDEDRAAETMARAAAALSESPLRFGTHFARGRIGPAVARTVRDL
                     GCDALITGAHRVEGRAEMPSHTEELLRATDIAVVVQP"
BASE COUNT          568 a         1514 c         1519 g          537 t
ORIGIN      
        1 gggcgttccc gcccccgacg acgattccac cgccctccac ctggcgaaca acctccgcgc
       61 cgagacgcgg atggacggga gcgtacgggg ggaattcctc accaccgcat ggcgcgccat
      121 cgagctgatg gacctcgatc acggcgccga ccaggccggc accgtgcgag tgatcggcta
      181 cctgctggcg cggcaggacg agccgggcgc ctacggcggc cgcaacgcgc gccccgagca
      241 cgccggtcgg agccttcccg gcttcttctc acccggcgat ccccagcggc cgatcgcgcc
      301 gatcacgctg cccaccggtg ccaccatcgg cgacgatgcc gacgcccgtt acgcggtgag
      361 ctgcctggcg ctccgggcgg cgctcaaggc ccggctcgaa aagcgccccc aggtgcacca
      421 gcacaccgag agcctcgccg cgctggcgca ggactggggc ggtccgatgc cgctcacgct
      481 cgcctgctcg acgctccacg ggctggcgct cgccgcgcag ccgatgcggg acgcgatccc
      541 cgggctcatc gcgcggatcg ccgcggcgga gtcgggaggc gtctggcccg acgcggacct
      601 cttccacgtg ctgcaggcgc tgctgtcggt ccccgacgcg gcggcgacgg cgctcatccg
      661 gcgggcggtc ccggcgctcg tcacgctgca gcggccggac ggcgggttcg acggcgagga
      721 gccgtacggg gaggagcggg gctggatcgc gacgcgggcg ttcctgcggg cgcggggggc
      781 gtaaggggtg ggaccacgga gggcgcggag ttcacggaga ggcacggagg aaagcaactt
      841 gacgtggtct cgcacgcgga gctccgtccg cacggccaca tcacgagcag ttctccgaga
      901 atctccgcgt tcctccgtgc cctccgtggt cccacgcccc ttcgctaccg cgatgccagc
      961 acccccgcgg cgcgccagat ccaccaggcg ccgacgatga tcccggcgag gcccacagcg
     1021 gacgcgatcc ggcggcccca cgcgagcgaa cggccggcgg cggcgcgcat cgcagcggcg
     1081 gccacgaagg caaagagcgt catccccgcg gtgacgccga cgccgaaggc gacgaggtac
     1141 ccgagcccca cgaggcggct ggcaatgagc gtgaccggca ccagcgccac cacgcccgcc
     1201 gtgccggcca ggccgtggag catcccgacc caggtgatgc cgtgcctatg gtcgtgggaa
     1261 tgggatgccg cgggcgccgg gtgcgggtgc gcgtgcccgg cgccggcgtg cccgtgatgg
     1321 tggaggtggg cgtggtcgcc gtgtccctcg ggagcatgca ggtgtaactt cgaggccgag
     1381 cgcagcgccc agagtccgag ccccacgagg agcagcccca ccacgatctc gccgagcgag
     1441 tcgtaccgcg ccggccagtg caatccgatg gcgaggagca ccccgcccgc caggcagacg
     1501 ctgatcgaat ggccaaggcc ccagcgggcg ccgaacccgg cggcggtggc gagcgccggg
     1561 cgccggctca cgaaggcggt caccgccagc atatggtcga cctccagcgc gtgcaggaag
     1621 cccaggacga tcgctgtgcc caacgtgacc cacgtgccca tgtcagtcct ttcagacagg
     1681 ccgttccgat gaccgacgcc gcccccgcac cgatccggct caccgcgttc tcccacggcg
     1741 ccggctgcgc ctgcaagctc ggcgccaccg agctgaccga ggtgctgcgg cacctgccgc
     1801 cgatcagcga tccccggatc ctggtggacg ccgccacccg tgatgatgcg gcggtctacc
     1861 ggctcgacgc cgaccgggcg ctcatcgcca cgaccgactt cttcacgccc atcgtcgacg
     1921 acgccttcga ctgggggcgg atcgccgccg ccaatgcgct ctccgacgtc tacgccatgg
     1981 gtggccggcc gctcttcgcc ctcaacctgg tggggtggcc ccgcacgtcg ttgccgttca
     2041 cgctcctcgg cgaggtcctc cgcggcgcag gccaggtggc ggcagcggcc ggctgcctcc
     2101 tcgtgggcgg ccactcggtg gacgacccgg agcccaagtt cggcctggcg gtggtcggcg
     2161 aggtgcaccc cgaccggatc ctcaccaacg ccgccgggca ggccggcgac ctgctcatcc
     2221 tcaccaagcc gctcggcacc gggatcctga ccaccgcgct caagcgggac cgggtcgggc
     2281 ccgaggcgct ggccccggcg gtcgagagca tggccacgct caacgacggg gcgatgcgcg
     2341 cggcgctggc gcacggcgtg cggtgcgcca ccgacatcac cggcttcggg ctcctcggcc
     2401 acctcggcaa tgtgctggcc ggaagcgggc tcggcgccga ggtgtgggtc gacgcgctgc
     2461 cgctccttcc cggggcgcgg gagctcgccg aggccggcgt cattcccggc ggcaccgagc
     2521 ggaattataa ggcggcggcg gggacgcgct gggcgcaaga cctttcccgg gcggagcagg
     2581 tgctctgctg tgacgcgcag acctcaggcg ggctcctgct cgcggtccgg ccggagcgag
     2641 cggaggcgct ggtggcggcg ctcacggccg agcggacgcc ggccgcggcg gtcatcgggc
     2701 ggctggtgga cggccctgcc gggagcgtct cggtcgagcg tcaacgatga cagatgtggg
     2761 accacggagg actcggagga acacggagat ccacggagaa ctgctcgtga tgtggtcctg
     2821 cggtcggagc tccgcgcgcg agaccacaat gggtcgcttg tctccgtgcc tctccgtgga
     2881 ctccgtgtcc tccgtggtcc caccctttct gaccggagcc tgaatgcgct ggacggcgga
     2941 acggatggac cagctggaac gcgcggtgcg ggacgggctc cgggtggcgc tctcgcggcg
     3001 cgggtcggag ttcatcgtcg tggcccggcg gatcacgtcg gtggcgcagc gtgacgcgct
     3061 ggtggggcgg ctgccgatga cgaacgagga gaagaccttc atcctggacg agctggacgg
     3121 gttctcgatc ctcggcgctt gacgccggcg gtccgcgccc gcaccctcaa ggtatgttca
     3181 cccgcctcct cgtcggcctc gacggctcga aaccctcccg ggtggcgctg gcacaggcca
     3241 tcgaactcgg caagcggttc cgcgcccggc tgctcctggc ccacgtgagc cgacccgccg
     3301 aggtcgcggc gacccagggc ccggggcgcg acaccggcgc cttcaccctc gcgatcctcg
     3361 acgaggcggc ggccgccgcg gccgaggcgg ggctccaggg ggagatgatc cagcgacggg
     3421 gcgaggtggt ggagcagctg atggcgctgg cccacgaggc cgacgcggtg ttcgtcggtc
     3481 gcgcgggcct cgatgccagg gaccccctcg gcccggagac ccgggccctg atccagcgct
     3541 gccccgtgcc ggtgttcgtg gcggccacgt cggtggcgca gttcgaccgg tgcgcggtgg
     3601 cctacgacgg cggcgccacc agccagcggg cgctggcctt cgcggccaga ttcgtcggca
     3661 tccgcggggg ccgcctcgag gtcgtgaccg ccggcgagga cgaggaccgg gcggcggaaa
     3721 cgatggcgcg cgccgccgcc gcgctttcgg agagcccgct ccgcttcggg acccactttg
     3781 cccgcggccg gatcggcccc gcggtggccc gcacggtccg cgacctcggc tgcgacgcgc
     3841 tcatcaccgg cgcgcaccgg gtcgaggggc gcgccgagat gccgtcgcac accgaggaac
     3901 tgctccgggc caccgacatc gcggtggtgg tgcagccgtg attgggaggt caaggcgggg
     3961 gaaggggcaa aaggagcagg cgggcgggaa agcgagaatg gagagagtgg tccggacgat
     4021 gagacgctag cccggggcat tcgcgccttc tcgctcttcc cgcccgcttt cccccttctc
     4081 cttttacctt tcccctcgtg ctgaccgcca tcgcccacct cgacgaaccc tgcctcgg
//
LOCUS       DASUAP010000023         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_314039, whole genome shotgun sequence.
ACCESSION   DASUAP010000023 DASUAP010000000
VERSION     DASUAP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314039"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFU45_00575"
     CDS             complement(<1..196)
                     /locus_tag="VFU45_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5357581.1"
                     /translation="MSFPAAASATSAGGPRPQLGVGVLILREGKLLLGQRRGSHGAGT
                     WAPPGGHLDSGESVEQCACRE"
     gene            complement(244..681)
                     /locus_tag="VFU45_00580"
     CDS             complement(244..681)
                     /locus_tag="VFU45_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357582.1"
                     /translation="MPDIRHQIQVAATPKAIYPLISSAEGFARWWAADAKHTGGASGV
                     IEVALFGRRSVYRLRPQTFLAPNQTSWQCESGEEWAGTLINFVVGPHAAGSVLRFTHG
                     GWAAETDFFITSNTTWGALLYRLRAVAEGREPGPLFTVDGQSY"
     gene            complement(744..1781)
                     /locus_tag="VFU45_00585"
     CDS             complement(744..1781)
                     /locus_tag="VFU45_00585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5357583.1"
                     /translation="MELGIYTFGETTPDRHGVTISTARRLHDLLEEVELADQVGLDVF
                     GIGEHHRADYSVSAPAVVLAAAAARTSRIRLTSAVTVLSSDDPVRVFQQFATLDLLSG
                     GRAEIMAGRGSFTESFPLFGYDLNDYDDLFAEKLGLLLTLRTAGRVTWSGRHRAPLRD
                     QPVFPRPIQEPLPVWVAVGGTPQSVVRAATLGLPMALAIIGGQPARFAPLVDLYRTAG
                     AEAGHDPATLRVGINSHGFIADDATRAADEAFPPYADTMGRIGRERGWPPPTRAHFDR
                     EVAPAGALLVGDPSRVTDKLLAEHEIFRNDRFLLQFSVGTMPHDRILRSIELFGTRVA
                     PAVRKALGSKA"
     gene            2006..>2237
                     /locus_tag="VFU45_00590"
     CDS             2006..>2237
                     /locus_tag="VFU45_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357584.1"
                     /translation="MSSCNTTLSGAKTGTYACAALGAWTSGTNWGGFELSLSNIDPVI
                     IKANISFPGDLHPGTFASSDTTVTGGVTLQQGG"
BASE COUNT          343 a          741 c          841 g          312 t
ORIGIN      
        1 cctctcggca cgcgcactgc tcgacactct ctcccgagtc gagatgtccg ccgggcggcg
       61 cccacgtccc cgcgccgtgc gatcctcggc gctgacccag gaggagcttc ccctcgcgga
      121 ggatcaggac gccgacgccg agctgggggc gggggccgcc ggccgaggtc gccgatgcag
      181 cggccgggaa cgacacgagg tcccgggccg cccgggacct cgaagagttc ccacctacgg
      241 cggtcagtac gattggccgt cgacggtgaa gagcgggccg ggctcccggc cttcggccac
      301 cgccctcagg cgatagagca gggcgcccca ggtggtgttg gaggtgatga agaagtcggt
      361 ctccgccgcc cacccgccgt gggtaaagcg gagcacggaa ccggcggcgt gcgggccgac
      421 gacgaagttg atgagcgtcc ctgcccactc ctcgccgctc tcgcactgcc agctggtctg
      481 gttcggggcc aggaaggtct ggggccgcag ccgatacacc gagcggcgtc cgaacagtgc
      541 cacctcgatc acgccggagg cgccaccggt gtgcttcgca tcggcggccc accagcgggc
      601 gaacccctcc gccgaggaga tcagcggata gatggccttc ggggtggcgg cgacctggat
      661 ctggtggcgg atgtccggca tcgggggcct cgcgggagtg caatggaaat accagtccag
      721 cgccacggtg gcaaccccgc cgttcaggcc ttcgacccca gggccttccg caccgccggc
      781 gccacccggg tgccgaagag ctcgatggag cggaggatcc ggtcgtgggg catcgtcccc
      841 acgctgaact gcagcaggaa gcggtcattc ctgaagatct cgtgctcggc caggagcttg
      901 tcggtcaccc gcgagggatc gcccacgagc agggcgcccg ccggggccac ctcccggtcg
      961 aagtgcgccc gggtgggggg cggccatccc cgctcgcggc cgatgcgccc catcgtgtcg
     1021 gcatacggcg ggaacgcctc atccgcggcg cgcgtcgcgt cgtcggcgat gaagccgtgg
     1081 gagttgatcc ccacccggag cgtggcggga tcgtgtcccg cctcggcgcc ggcggtgcgg
     1141 tagaggtcga ccagcggcgc gaagcgggcc ggctgcccgc cgatgatggc gagcgccatc
     1201 gggaggccga gcgtggcggc gcggaccacc gactgcgggg tgccgccgac ggccacccag
     1261 accgggagcg gctcctggat cgggcggggg aagaccggct ggtcgcggag cggagcgcgg
     1321 tggcgccccg accaggtgac ccgtcccgcc gtgcgcagcg tgagcaggag cccgagcttc
     1381 tcggcaaaga ggtcgtcgta gtcgttcagg tcgtaaccga agagcgggaa cgattcggtg
     1441 aacgatcccc gacccgccat gatctccgcc cgccccccgg agaggaggtc gagcgtggcg
     1501 aactgctgga agacgcggac cgggtcgtcg gagctcaaga cggtgacggc gctggtgagc
     1561 cggatgcggc tggtccgcgc cgcggccgcc gcaaggacca ccgcgggtgc ggacaccgag
     1621 tagtcggcgc ggtggtgctc gccgatgccg aagacgtcga gcccgacctg gtcggcgagc
     1681 tccacctcct cgaggaggtc gtggagccgc cgggcggtgc tgatggtgac gccgtgacgg
     1741 tcgggcgtgg tctcgccgaa ggtatagatg ccgagttcca tggggaccag atcgtgggga
     1801 agatgagtgc accggcggag ttgacggcgg cgccgggacg ccggaagtta gtggagggct
     1861 tgaggccctg atgtcaaacg atgttggtcg tgatatccgg gagcgatctg cgatgcgata
     1921 tccggcggtc gccacgatgg tcctcgtggc ttgcggtgcg ttgggcgctt cgagttgcgg
     1981 atcgggcacg tcgccatccg gcccggtgag cagctgcaac accacgctga gcggcgcgaa
     2041 gaccgggacc tacgcgtgcg ccgccctcgg tgcctggacc agcggcacca attggggtgg
     2101 gttcgagctt tccctgagca acatcgatcc ggtcatcatc aaagcgaaca tctcattccc
     2161 cggagacctg cacccgggga ccttcgcttc gtcggacacc acggtgaccg gcggcgtgac
     2221 gctgcagcag ggcggta
//
LOCUS       DASUAP010000024         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_682037, whole genome shotgun sequence.
ACCESSION   DASUAP010000024 DASUAP010000000
VERSION     DASUAP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682037"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VFU45_00595"
     CDS             complement(<1..1071)
                     /locus_tag="VFU45_00595"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5357585.1"
                     /translation="MPFSVFKLHPDLLRGIKELGFTRPTPIQSDAIPPALEGRDLLAC
                     AMTGSGKTAAFLLPILDRLMTKPRRVTRALVLAPTRELAAQIVEELNALAVHTPITAA
                     AVFGGVGMGPQEHAFRSGVDVIVATPGRLLDHFRMPYAKLAGLEHLVLDEADRMLDMG
                     FLPDIRKVLRHLPTKRQTFFFSATLPPEIQALSREMLKDPATINIERPSAPATGITQA
                     VYPVGRELKSTLLLELLKRKEVGDALVFTRTKHRANRLAEQLVKAGIKAERIHGNRSQ
                     SQRTEALAGFKNGKYRVLVATDIAARGIDIEALQHVINFDVPAVPEDYIHRSGRTARA
                     ERTGFAFTLVSPDEEGDLKRIER"
     gene            complement(1110..1364)
                     /locus_tag="VFU45_00600"
     CDS             complement(1110..1364)
                     /locus_tag="VFU45_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357586.1"
                     /translation="MPSNKNEQRRIILRGRQFHFVSYDATPANEKRGELAVPPMWYLM
                     NEGHRRPVMPHVPGQDPVTLERALSRWVDEHIFSQATSGA"
     gene            1623..2522
                     /locus_tag="VFU45_00605"
     CDS             1623..2522
                     /locus_tag="VFU45_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEU5357587.1"
                     /translation="MLNPVLRRRLAALHRWSALAMAPVIAVILVTGIILAFRPILGTP
                     QDEHPSGAVLDPGRLLAVLDSIGGTADFVLVQDDRRSITVFTGPRQPPKTWELATGRE
                     VATPPFTPPDFFDKVERVHKDLWGGLGFLVTLASIAMVVLIVLGPILARPSRGRKNVL
                     GWHIWGGWFAWPLLALLPVSLVMMKLHTPFGRRRAGPPPSIAAVLGTAARTLDLTHLD
                     GAQRFPGGAIVVIGGVHGEGRYVVRGDSVQPLESGMSRLGKELHTGTWAGPWSGALNV
                     VAALGLLVMLWTGTVAWVRKLRR"
     gene            complement(2649..>2949)
                     /locus_tag="VFU45_00610"
     CDS             complement(2649..>2949)
                     /locus_tag="VFU45_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEU5357588.1"
                     /translation="YVEALHYELNERRIPFDSEVLLHLRYKDRWMTTSYRADLVCYGT
                     IIVELKARREVGGPEDAQVLNYLKASGLSRGLLLNFGTPRLEYRRYVWSHRSSGP"
BASE COUNT          435 a          988 c         1031 g          495 t
ORIGIN      
        1 gcgctcgatc cgcttgaggt cgccctcctc atcgggcgag accagcgtga aggcgaatcc
       61 ggtccgctcc gcgcgcgccg tccggccgga gcggtggatg tagtcctcgg ggaccgccgg
      121 cacgtcgaag ttgatgacgt gctgcagcgc ctcgatgtcg atgccgcgcg cggcgatgtc
      181 ggtggccacc agcacccggt acttgccgtt cttgaagccg gcgagcgcct cggtgcgctg
      241 gctctgggaa cgattcccgt ggatgcgctc cgccttgatc cccgccttga cgagctgttc
      301 cgcgaggcgg ttggcccggt gcttggtacg ggtgaagacg agcgcgtcgc ctacttcctt
      361 gcgcttgagg agctcgagga ggagggtcga cttgagctcg cgccccaccg gatacaccgc
      421 ctgcgtgatc cccgtggccg gtgcggaggg gcgctcgatg ttgatcgttg ccggatcctt
      481 gagcatctcg cgcgagagcg cctggatctc gggcggcaac gtggcgctga agaagaacgt
      541 ctgccgcttc gtgggcaggt gccggagcac cttgcggatg tcggggagga aacccatgtc
      601 gagcatccgg tccgcttcat cgagcacgag gtgctcgagc cccgcgagct tcgcgtacgg
      661 catccggaag tgatcgagca gccggcccgg ggtggcgacg atgacgtcga ccccgctccg
      721 gaaggcgtgc tcctgcggcc ccatccccac gccgccgaag accgccgcgg cggtgatggg
      781 ggtatggacg gcgagcgcat tgagctcctc gacgatctgg gccgccagct cccgcgtcgg
      841 ggccagcacc agcgcgcggg tcacccggcg cggcttggtc atcaggcggt cgaggatcgg
      901 gaggaggaag gcggcggtct tgccgctccc ggtcatcgca caggccagga ggtcgcgccc
      961 ctcgagggcc ggcgggatcg catcggactg gatcggagtg gggcgggtga agccgagctc
     1021 cttgatgccg cggagcaggt cggggtggag cttgaagacg ctgaacggca agggacttcc
     1081 tgtcgggctt gaagggtggg ggacagcgtt cacgcgccgc tcgtggcctg ggagaagatg
     1141 tgctcgtcca cccagcgact gagggcgcgc tcgagcgtga ccggatcctg cccgggcacg
     1201 tggggcatca ccggccggcg gtgtccctcg ttcatcaggt accacatcgg cggcacggcg
     1261 agctcccccc gcttctcatt cgcgggggtc gcgtcgtacg acacgaaatg gaattggcgg
     1321 ccacgcagga tgatccggcg ctgttcgttc ttgttggacg gcattgcgac acgctcacac
     1381 gcgcgggaca ggggcggtgg gagcgcgcgc aagccaccgg acgcgggcgg aagcccacgg
     1441 ttagaaagat aaccccgtgt acccccttcc cgagcccctg aggcgccggg agggccgagg
     1501 cgggggcggg ggtaccctcg cgccaacctg gtgacctctt cgcgtttgtt ccattgtgaa
     1561 actttcacac tgccgctccg tagaccggtg gacccgagcc ccgcacctca accaccgagc
     1621 cgatgctcaa tcctgttctg cgccgccgcc tcgcggcact gcatcgctgg tccgcactcg
     1681 cgatggcccc ggtcatcgcg gtcatcctgg tcaccgggat catcctcgcc ttccgcccga
     1741 tcctcgggac gccgcaggac gagcacccct ccggcgccgt gctcgacccc ggccgcctct
     1801 tggccgtcct cgacagcatc ggtggcacgg ccgacttcgt cctcgtgcag gacgaccgca
     1861 ggtcgatcac ggtcttcacc ggcccgcggc aaccgccgaa gacttgggag ctcgcgaccg
     1921 ggcgggaggt cgcgacgccc ccgttcacac cccccgactt cttcgacaag gtcgaacgtg
     1981 tccacaagga cctctggggt gggctcgggt tcctcgtgac cctggcctcg atcgcgatgg
     2041 tggtcctcat cgtgctcggg ccgatcctgg cccgtccctc ccgcggccgg aagaacgtgc
     2101 tcggctggca catctggggc gggtggttcg cctggcccct tctcgcattg ctgccggtgt
     2161 cgctggtcat gatgaagctc cacaccccgt tcgggcgcag acgcgccggc ccgccgccct
     2221 cgatcgccgc cgtcctgggc acggcggcaa ggaccctgga cctgacgcac ctcgacggcg
     2281 cgcagcggtt ccccggcggc gccatcgtgg tgatcggcgg ggtccacggc gagggacgct
     2341 acgtggtccg tggcgactcc gtgcagcctc tggagtcggg gatgtcgcgc ctcggcaagg
     2401 aactccacac cggcacctgg gccggtccct ggtccggcgc gctcaacgtc gtcgcggcgc
     2461 tcggcctgct ggtcatgcta tggacgggaa cggtggcgtg ggtcaggaag ctgcggcggt
     2521 gagggatccg catctgacgc agattgcgca gatgcgcgca cgacgattga gtcactgaca
     2581 atcagtaaag ttcgcagacg gcgccgatcc tcgccgggtg atctgcgcaa tctgggtcat
     2641 ctgcggattc aaggaccgga cgaccggtgc gaccagacgt accggcgata ctccaggcgg
     2701 ggtgtgccga agttgaggag cagcccccgg gaaaggccgc tggccttcag atagttgagg
     2761 acctgggcat cctccgggcc gcccacttcg cgtcgagcct tcaactcgac gatgatcgtg
     2821 ccgtagcaga cgagatcggc ccgataactg gtggtcatcc agcggtcctt gtacctcagg
     2881 tgcagcagca cctcgctgtc gaacgggatc cttcgctcat tcagctcgta gtggagcgct
     2941 tccacgtag
//
LOCUS       DASUAP010000025         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_191460, whole genome shotgun sequence.
ACCESSION   DASUAP010000025 DASUAP010000000
VERSION     DASUAP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191460"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..2141
                     /locus_tag="VFU45_00615"
     CDS             258..2141
                     /locus_tag="VFU45_00615"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU5357589.1"
                     /translation="MDPKLLRRLAWTGAAFTFLLIVLGGIVRITGSGLGCGEHWPNCN
                     GRWFPPLDLPTLIEISHRWVAALVTITVTSVGIVAWLRHRNEPRIRNPATVAFVLLVV
                     QVLLGAITVKLTLPPSVVVVHLANAMLVLAALLVTAMRASTTQEAVPAAERHPGHPLA
                     LVTAGLGFIVILFGALTANLHAGWYCSGFPLCDGKLSPPTVPLALIHWIHRLLAFTFV
                     GFVMALAWTARRRRGPAGLRYRRMTAALLVVSLAQVAVAAAMVLNTLPPALRALHLFT
                     GTSVWAVLVVLLFFSRRTPAVEAESAETAGDARPSVLRDLVTLTKPRIISLLLVTTIF
                     PMFITGHGVPSVGLVIWVVIAGYLMAGGANAINMWFDRDIDDRMSRTKLRPVPSGRMP
                     AWGALVFGMALGASSFALFWAYVNPLSAFLALAGLLFYVLVYTIWLKRWTPQNIVIGG
                     AAGAFPPLVGWAAMAGRLDLTAIYLFAIIFYWTPPHFWALALIKQGEYAKAGIPMMPL
                     VQGEHRTKLQMLGYTVMLLPLTLLPWISGDLGLFYAAAAVLLGARLLWYCIRLLREPA
                     VTPTAWKMYKYSLLYLFLLFAAMGVDRAVPFGHGNRAPEVIELPAPPPSAAPTSPETS
                     HGH"
     gene            2176..>2939
                     /locus_tag="VFU45_00620"
     CDS             2176..>2939
                     /locus_tag="VFU45_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU5357590.1"
                     /translation="MAPPGQLARLWPRVRPYRGALSLAFATLVLSGAISLAFPQLAGS
                     LVDAAFVRHSRTYLDRIALGLVALFTVQAALNYVQTYLLTATGERAVAGLRRELFGKL
                     LEMPPGFFADRRVGELTSRLNVDIGLLQSAMSTQVAEFFRQTMTLVGGILILTLSQPH
                     LMLTALLVAPLVVGSAVFFGRRLRKVSKGLQDKVADANALSEEALSQIRTVQSFVQEP
                     TERGRYAAKIQAIITTALGRAHVRGIFFGVITLVTF"
BASE COUNT          365 a         1097 c          968 g          509 t
ORIGIN      
        1 cctcgaaccg tccgctttgg gcgtgctgca aatcatctgg gattgtcacg gcggatgatg
       61 gaaagaaggc cccgagatct gcgattggca acgggccttc cggtgccggc ccgcagacgt
      121 gccaggagcg gccgagaagc gctcggggcc cggctctccg ccttgacatc acgcgttgaa
      181 acacggtttt ttggggtcgg accgaccctc gcgcgcgcct gagcacccgc gtccaatcgc
      241 ctgccggaaa aggagttatg gaccccaagc tgcttcgacg cctggcctgg accggcgctg
      301 cgttcacctt cctgctcatc gtcctgggcg ggatcgtccg gatcaccggc tccgggctcg
      361 gctgcggcga gcactggccc aactgcaacg gccgctggtt cccccccctc gacctcccga
      421 ccctgatcga gatcagccac cgctgggtgg cggccctcgt caccatcacg gtgacgtccg
      481 tcgggatcgt ggcctggctc cgtcatcgga acgagccccg gatccggaac cccgccaccg
      541 tcgccttcgt gctcctggtg gtccaggtcc tcctcggcgc catcacggtc aagctcaccc
      601 tcccccccag cgtcgtcgtg gtccacctgg ccaacgcgat gctggtgctc gccgccctgc
      661 tggtcacagc catgcgcgcc tccacgaccc aggaggccgt ccccgccgcc gagcggcacc
      721 cgggccaccc gctggcgctc gtgaccgcgg ggctcgggtt catcgtcatc ctcttcggcg
      781 ccctcaccgc caacctgcac gccggctggt actgctcggg gttcccgctc tgcgacggca
      841 agctctcgcc gccgacggtc ccgctggcgc tgatccactg gatccaccgg ctgctggcgt
      901 tcaccttcgt cggctttgtg atggccctgg catggaccgc ccggcgccgc cggggtcccg
      961 ccggcctccg ctaccggcgg atgacggcag cgctcctggt ggtgtccctg gcccaggtgg
     1021 cggtggccgc ggcgatggtg ctcaacaccc tgcccccggc gctccgggcg ctgcacctct
     1081 tcaccggcac gtcggtgtgg gcggtgctgg tggtgctgct cttcttctcc cgccgcaccc
     1141 cggcggtcga agcggagagc gcggagacgg cgggcgatgc gcgcccctcg gtgctgcgcg
     1201 acctggtgac cctcacgaag ccgcggatca tctcgctcct gctcgtcacc acgatcttcc
     1261 cgatgttcat caccggccac ggtgtcccgt cggtggggct cgtgatctgg gtggtcatcg
     1321 caggatacct gatggcgggt ggcgccaacg ccatcaacat gtggttcgac cgggacatcg
     1381 acgaccggat gagccgcacc aagctccggc cggtgccgtc ggggcggatg ccggcgtggg
     1441 gggccctggt gttcgggatg gccctcggcg cctcgtcctt tgcgctcttc tgggcctacg
     1501 tcaacccgct ctcggcgttc ctggcactgg ccgggctcct cttctatgtg ctggtgtaca
     1561 ccatctggct caagcgctgg acgccccaga acatcgtgat cggcggggcg gccggcgcct
     1621 tcccgccgct ggtggggtgg gcggcgatgg ccggccggct cgacctcacg gccatctacc
     1681 tctttgcgat catcttctac tggaccccgc cccacttctg ggcgctcgcg ctcatcaagc
     1741 agggggagta cgccaaggcc gggatcccga tgatgccgct ggtgcagggg gaacaccgga
     1801 ccaagctcca gatgctgggc tacaccgtga tgctcctgcc gctcaccctg ctcccgtgga
     1861 tctcgggcga cctggggctc ttctacgcgg cggcggcggt gctcctgggc gcccggctcc
     1921 tctggtactg catccgcctt ctccgggagc cggcggtgac gccgacggcg tggaagatgt
     1981 acaagtactc gctcctgtat ctcttcctcc tcttcgcggc gatgggcgtc gaccgggcgg
     2041 tgccgttcgg gcacggcaac cgggcaccgg aggtgatcga actccccgcc ccgccgccct
     2101 cggccgcgcc aacgagcccg gagacctccc acggccactg acgcgcccgg cggcggagag
     2161 ccgcgccgga ggctcgtggc cccgccggga cagctggcgc ggctctggcc ccgggtccgg
     2221 ccctaccggg gggccctctc cctcgccttt gccacgctgg tcctgagcgg ggccatctcg
     2281 ctcgccttcc cgcagctcgc cgggtccctg gtcgacgccg ccttcgtccg ccacagccgc
     2341 acctacctcg accgcatcgc cctgggcttg gtagcgctct tcaccgttca ggccgcgctc
     2401 aactacgtcc agacctacct cctcaccgcc accggcgaac gggccgtggc ggggctccgc
     2461 cgggagctct tcgggaagct cctcgagatg cccccggggt tctttgccga ccggcgggtg
     2521 ggcgagctca ccagcaggct taacgtggac atcgggctcc tccagtcggc gatgagcacc
     2581 caggtggcgg agttcttccg ccagacgatg acgctggtgg gcgggatcct catcctgacc
     2641 ctgagccagc cccacctgat gctcaccgcc ctcctcgtgg cgccactggt cgtgggctcg
     2701 gcggtgttct tcgggcgccg gctgcggaag gtgtcgaagg ggctccagga caaggtggcc
     2761 gacgccaacg ccctctccga ggaggcgctg agccagatcc ggacggtgca gagcttcgtc
     2821 caggagccga ccgagcgggg ccgctacgcc gccaagatcc aggccatcat caccacggcg
     2881 ctcggccggg cgcacgtgcg ggggatcttc ttcggggtca tcacgctggt cacgttctg
//
LOCUS       DASUAP010000026        10984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1036396, whole genome shotgun sequence.
ACCESSION   DASUAP010000026 DASUAP010000000
VERSION     DASUAP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10984
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036396"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(536..1399)
                     /locus_tag="VFU45_00625"
     CDS             complement(536..1399)
                     /locus_tag="VFU45_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5357591.1"
                     /translation="MRDDPPRFGEAGGWQASPRSSGAALAQPEPAAVTPGPRGKVAVV
                     TGGATGLSRAICHEFARLGCHVAFCFVELPGRDVTEMALLTETGLLGMGVQVYAARCD
                     VRDPAAVQGFIDETARRFGGFHYLVNNAGIAVDGALWRLTDEAWNEVMATNVTGAFNC
                     IRAATPHFRARRYGKVVSISAHQAARPGFGVANYAASKAALEGLTRAAAVELGPANVN
                     VNAVAPGFIRTERMMMLPREVVERARMHAVLGRVAEPEDVARVVAFLCSEEARHITGQ
                     TIVVDGGLSLE"
     gene            1539..3002
                     /locus_tag="VFU45_00630"
     CDS             1539..3002
                     /locus_tag="VFU45_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5357592.1"
                     /translation="MIRALTPVLLAALVATSLAAQEPFTPAVARAPLAALADDSMGGR
                     LVGTAGARAAAEYLAGRLSALGLAPAGDSGGYLQHIPLERRGIADSSTVLLDAGSRAV
                     TLAWGQTFAVEWGRPDSSKTYDAIWGDLYTRAGIDSTFKSAAGKFLVINVPDGTGLLV
                     QGFPPTGRPAGIIALFSTARFRVRAARTARPAPVTFIGANPGNPPLVIIADRDSVAAA
                     LGSVAQGPAGTPAPFRITPRLVASIIPVDGWNVVATRPGGDPGRRGEYVLYTAQFDHV
                     GRADDPAGFCRAVGADQVCNGAGAVSGAVAELLGARRAATDPAPHRSMLFAWLGAEEA
                     GSLGLRWLLAHPPAPTDSIIAVIGLGPVGRGAADSLEEGGVRELAPDLLRVVDSVAQS
                     DSPPFALIGRFERLDDPRRIYCRTPAAGLATMGIPSITFSSGYYPEFRRADDSVALVD
                     ADKVARVGRLAYEVGMALGDARPRLATDSTLSRTLCR"
     gene            complement(2999..4171)
                     /locus_tag="VFU45_00635"
     CDS             complement(2999..4171)
                     /locus_tag="VFU45_00635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5357593.1"
                     /translation="MRAFERIPRTVRGLALVSLFNDFASEMVYPLLPAFVTTVLGAGA
                     VTLSALDGAADLTAAVLKWASGWLSDRPGWRKPLILAGYGTAVLIRPLIAFANSAGQV
                     IGFRVIDRVGKGLRTPPRDALIADSAPPELHGRAFGFHRGADHLGAVAGSVAAWWFLS
                     RHAEVRQVIEWSAVPGVVVLVALAVVLRGKGGTARTDDDPPVAPAPEPGRAYWAPVLT
                     LAVFALARLPETLLLLRLQQVGVALPLIPLAWAGLHMVRSGASYPGGRLNDHLGPSAG
                     ILVGAAVYGATLYALAVAASPLASLGAFLALGLAAGATESAERTLVARLSPRRQGRGF
                     GGYHAVTGFAALPAALLFGAAYAHAAGTALTLSAGLTVLSAVLWVVVGRRVPVPAR"
     gene            complement(4168..5727)
                     /locus_tag="VFU45_00640"
     CDS             complement(4168..5727)
                     /locus_tag="VFU45_00640"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEU5357594.1"
                     /translation="MSGLGGLCRSYLDVRWTFDPAQGTRAGNEASDALLPMLDAESVR
                     LQLATLRSLAGAVEDLSLDDEEEIDRTGLLDDLRTTIFRFESEVPHRHNPAAWLSSVA
                     QAWQSLLAHPGRVPELRAQAALARLQGAPAVLSAAAATLKAPAVLFVDEAVALAPAVA
                     LLPARLARVAAGWLPGIADQLGAAAALAEGAVRDFAARLTSGTVPTADPAALAVGEDE
                     FEHRLHFEHALHASAPELWRWGQRSLEEQTAAVVQAAERVEPGAAPRALAERLRRERP
                     AAGDAQLVFLRTVARARKFVDDRGLLVAPSGDVRLLDTPEFLRPIVPVAGYEAPTVVY
                     LTPGPTVAREELVHVALREVCPGRHLHRAHSAGLRSDVRRLVSTPVSVDGWALYATDL
                     MFRAGFAESAEEEFFHQLALLHAAALVVVDVGLHTRGMAPAEAISFLMEHLPLDRSQA
                     LADVRRVAAWPTYGLAAAAGRREIRQLHDAWRARAGVDAPIKAFHQALLDYGGLPVSL
                     ARWGMDLGLDE"
     gene            complement(5756..6103)
                     /locus_tag="VFU45_00645"
     CDS             complement(5756..6103)
                     /locus_tag="VFU45_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5357595.1"
                     /translation="MDLPYRVEKPWGYELIWARTDRYVGKILHVKAGQALSVQYHHRK
                     DETMHLLSGEMIFRSGSEGQLTERRVAAGTSLHIPPPLVHQVEAVTDCDILEASTPEL
                     DDLVRVTDRYGRR"
     gene            complement(6103..6678)
                     /locus_tag="VFU45_00650"
     CDS             complement(6103..6678)
                     /locus_tag="VFU45_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5357596.1"
                     /translation="MFTPPGGERRDFLRGVVGKYLDALVKRTEDRVVQRRFARPPGAL
                     PEVGFLAACTRCNACADVCPPKAIRRAETDAGLAAGTPFIDPLLQPCVACPEMPCAAA
                     CPTGALVVPAGKWAGYRLNLVELVPERCITFHGSSCGVCAQACPVGSSALAMDADGHP
                     VLKAEGCVGCGVCVRACVTAPSSFTIRSLEA"
     gene            complement(6807..7343)
                     /gene="atpH"
                     /locus_tag="VFU45_00655"
     CDS             complement(6807..7343)
                     /gene="atpH"
                     /locus_tag="VFU45_00655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEU5357597.1"
                     /translation="MSTIGVARNYAEALFALAERDGLAAEYAELIDLVAGAVELAPRV
                     EAVLMSPRVTKAEKGRILTAALPKAPKAFRAFLVAVVKRGRQRLLRQIATQYLVLLDV
                     KLDRVRARVVLARAADAAQQKEIAAALTRALGKAVIPSFEADATILGGAVVQVGDRRY
                     DGSVRRKMATLRRALVSK"
     gene            complement(7340..7900)
                     /gene="atpF"
                     /locus_tag="VFU45_00660"
     CDS             complement(7340..7900)
                     /gene="atpF"
                     /locus_tag="VFU45_00660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU5357598.1"
                     /translation="MRSVWLAGILTALGATPLMAEGGGLLTVDGGLMVWTLVVFGLFF
                     LILKRFAWPVVLGAVEAREKKLEEQIAQAAKDREAAATLLAEQQKLLADARGQVVTIL
                     NDAKAAAERERQLGLEKARAEADDLMARARREIEAEKDRAVVELRREAVDLALAAAGK
                     LIGQRLQGEQDRKIVQDYLATLGPSK"
     gene            complement(7963..8241)
                     /gene="atpE"
                     /locus_tag="VFU45_00665"
     CDS             complement(7963..8241)
                     /gene="atpE"
                     /locus_tag="VFU45_00665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEU5357599.1"
                     /translation="MLHMLPLLQAAADTSVGKGYGLIGAGLAAGLAVLGAGVGIGRIG
                     GQAVEGMARQPENSAQIQTAMIIAAALVEGVALFAAVVAFVMQGKISF"
     gene            complement(8428..9393)
                     /gene="atpB"
                     /locus_tag="VFU45_00670"
     CDS             complement(8428..9393)
                     /gene="atpB"
                     /locus_tag="VFU45_00670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU5357600.1"
                     /translation="MNRLTRGIAIVLALAFGAGAAPVVAQGPAAPEHAGVPSREIDIM
                     HHLGNAHAIEYPCFEPGLTCEAELPQWKPVQVGPLAIDFSPTKHLVWMLLATVLALLM
                     LLLVSRSVARAEAAGRPATGFAGAIEAMALWVRQEVIVPNVGHHGEGYVPYLLSLFFF
                     ILFSNLLGLLPYGAPATGNIAVTGALAILALVIIEISGMRSLGTKGYLGTIFFVPHGL
                     PGGIGGAFMKGVLLIIMTPIEIIGKLAKPFALAVRLFANMTAGHVVILALLGLTFFFH
                     SYTVGVGASVLATGIMLLELFVAFLHAFVFTLLTAVFIGLIRTSH"
     gene            complement(9390..9764)
                     /locus_tag="VFU45_00675"
     CDS             complement(9390..9764)
                     /locus_tag="VFU45_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357601.1"
                     /translation="MTSDSRRYVIGVAAVAAAAGAVALALPGESGRGAWFGLTLALAV
                     QAPLGWWLVGTLGTGRFLTAWIVGMGARVGLLALAGFVILPVLRWPMGPGLVGLAAVL
                     VVLLGVEVFVAMAAGRSKIEAR"
     gene            complement(9767..9994)
                     /locus_tag="VFU45_00680"
     CDS             complement(9767..9994)
                     /locus_tag="VFU45_00680"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEU5357602.1"
                     /translation="MTSTPPGRGRAPDALRYAGLGIQLAVTVGALAWLGQWLDRRFGT
                     GGIITVVLVLVGFGGMMASLLKELRERSGRK"
     gene            10223..>10984
                     /locus_tag="VFU45_00685"
     CDS             10223..>10984
                     /locus_tag="VFU45_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5357603.1"
                     /translation="MSPSDDITALAQLAQAIQSLRAQVAARVVGQRDAVDGMLTAVLS
                     GGHALLVGVPGLAKTLMVATLAEALQLSFSRVQFTPDLMPGDITGTDVLEEDPASGRR
                     VFRFLRGPIFANVVLADEINRTPPKTQAALLQAMQEHRVTAGGTTYDLPAPFFVLATQ
                     NPIEQEGTYPLPEAQLDRFMLELRIGYPSRAEEEAIVERTTGAATAPVTPVLDAATIL
                     EMQGLVRRIPVARSLISSAVKLARMTRPDDADAPAV"
BASE COUNT         1658 a         4109 c         3821 g         1396 t
ORIGIN      
        1 gagggcgagc ggcaccaccg agagggtctg gccagcgagt gcgttggtgg agagggggag
       61 gttgacgatc tgctcgggct tcggggccgg ttcagcccgg tgcccgcacg ccgagaggag
      121 cactgccaag ccgatgaggg acaaggactt aggcaatcgc gacctgccgg gttggagcgg
      181 aaggagactg gttacggggg aaaggtaaat ggagggtggg cgagcgacta ggtacgaggt
      241 gcggaaaagg aggggcgagg agcggggtac aacgtacgag ggtcaaggtt cggggtactc
      301 caccgatggc gaggagccga ggcgcatccc tcaggatgac gcacggagga gatcacgatc
      361 ccacgggcgc ggcgtgccgc gcccctgcgt ctgacgtctc acctctcacg tccccaaaga
      421 atggccaggc cccgatcgcg atcccgaccg gggggcccgt cacgtcttcc gtctcacgtc
      481 tcacgtctta cgtctcacgt cccgtgacct gccgctcgcg cgtcgaacct cgcacctatt
      541 cgagcgacaa ccccccgtcc accacgatgg tctgcccggt gatgtgccgc gcctcctcgg
      601 aacagaggaa ggccacgacg cgggccacgt cctcgggctc cgccacccgc cccagcacgg
      661 cgtgcatccg ggcccgttcg acgacctccc gggggagcat catcatccgc tcggtgcgga
      721 tgaaccccgg ggccaccgcg ttcacgttga cgttggcagg gccgagctcc acggcggcgg
      781 cgcgggtgag cccctcgagc gccgccttgc tcgcggcgta gttggcgacg ccgaaccccg
      841 gccgcgcggc ctggtgggcg ctgatcgaca cgaccttgcc gtagcgccgg gcgcggaagt
      901 ggggcgtggc ggcgcggatg cagttgaagg cgccggtcac gttggtggcc atcacctcgt
      961 tccacgcctc gtcggtcagg cgccagagcg cgccgtccac cgcgatcccc gcgttgttga
     1021 ccaggtagtg gaaccccccg aagcgccggg ccgtctcgtc gatgaatccc tggaccgccg
     1081 cgggatcgcg cacgtcgcag cgggcggcgt agacctggac acccatcccg aggagcccgg
     1141 tctcggtgag gagcgccatc tcggtcacgt cgcggccggg cagctccacg aagcagaacg
     1201 ccacgtggca gccgagccgg gcgaattcgt ggcagatggc gcggctgagc ccggtggcgc
     1261 cgccggtcac cacggcgacc ttcccccggg gccccggggt gacggccgcg ggttcgggct
     1321 gggccagtgc ggcgccggag gagcgcgggg aggcctgcca gccgccggcc tcgccgaagc
     1381 gaggagggtc gtcacgcatc gtgacccgaa ggtacgggcc ggccacggga tggcaagaga
     1441 cgagtgcacc tcttctcccc acctccgccc ccgatacttt tccccgcacc atgtgccgca
     1501 ccgatcccag gatcccatcg aaccttccgg agcatccgtt gatccgcgcg ctcactcccg
     1561 tcctgctggc cgcgctcgtc gccacttcgc tcgccgccca ggagccgttc acgccggcgg
     1621 tggcccgggc tccccttgcc gcgctggccg acgactcgat gggcggccgc ctggtcggca
     1681 ccgccggcgc gcgcgcggcc gccgagtacc tggccgggcg cctcagcgcg ctcggactcg
     1741 ctcccgccgg ggacagcggc ggttacctgc agcacatccc gctcgagcgg cgcggcatcg
     1801 cggactcgag caccgtgctc ctcgacgccg gcagtcgagc ggtgacgctc gcgtgggggc
     1861 agacatttgc ggtcgagtgg gggcggccgg acagttcgaa gacgtacgat gccatctggg
     1921 gcgatctcta cacccgggcc ggcatcgaca gcacgttcaa gtcggcggcc gggaagttcc
     1981 tcgtcatcaa cgtgcctgac gggaccgggc tcctggtcca gggattcccg ccgacgggcc
     2041 gccccgccgg gatcatcgcc ctcttttcga cagcgcgctt ccgggtgcgc gccgcccgga
     2101 cggcccgacc ggcgccggtg accttcatcg gcgccaatcc ggggaatccc ccgctggtca
     2161 tcatcgcgga ccgggacagt gtcgcggccg cgctcggaag tgtggcccag ggacccgccg
     2221 gcacgccggc gccgttccgc atcacgccga gactcgtcgc ctcgatcatc cccgtggacg
     2281 gctggaacgt cgtggcgacg cgccccggcg gcgaccccgg gaggcgcggc gagtacgtgc
     2341 tgtacaccgc gcagttcgac cacgtggggc gggccgatga tccggcgggg ttctgtcgcg
     2401 cggtgggggc cgaccaggtc tgcaacggcg cgggtgcggt gtcgggggcc gtcgccgagc
     2461 tcctcggggc ccggcgcgcc gccaccgatc cggcccccca ccgctcgatg ctctttgcct
     2521 ggttgggcgc ggaggaggca ggttcgcttg gcctccgctg gctcctcgcg catccgccgg
     2581 cgccgaccga cagcatcatc gccgtcatcg ggcttgggcc ggtgggacgc ggagccgccg
     2641 attcactgga ggagggcggt gtacgtgagc tcgcgccgga cctgctgcgg gtggtcgatt
     2701 cggtcgcaca gagcgactcg ccgccctttg cgctgatcgg acgattcgag cggctggacg
     2761 accctcgtcg gatctactgc cgcacaccgg cggccgggct ggcgacgatg gggatcccgt
     2821 cgatcacctt ttcgagcggc tattacccgg aattccgccg cgccgacgac agcgtggcgc
     2881 tggtggacgc ggacaaggtg gcccgagtgg ggcgcctggc ctatgaagtg ggaatggcgt
     2941 tgggggacgc acggccgcga ctggccacgg actcgacact ctcgcgtacg ctctgtcgct
     3001 agcgggccgg caccggtacg cgccgtccga ccacgaccca gaggaccgcc gacaacacgg
     3061 tgaggccggc cgagagcgtg agtgcggtgc ccgccgcgtg cgcgtaggcg gcgccgaaga
     3121 ggagtgccgc cggcagggcc gcgaaacccg tcaccgcgtg gtacccgccg aacccgcgcc
     3181 cctggcgccg gggggaaagc cgcgccacca gcgtccgctc cgccgactcg gtggcaccgg
     3241 ccgcgaggcc cagcgccagg aacgcgccca gcgacgcgag cggcgacgcc gcgaccgcaa
     3301 gtgcgtagag ggtggcgccg tagaccgccg cccccaccag gatcccggcg ctcggcccaa
     3361 ggtggtcgtt gagccggccg cccgggtatg acgcgccgct ccgtaccata tggaggccgg
     3421 cccacgcgag cgggatgagc ggcagcgcca cgcccacctg ctgcagacgc aggagcagga
     3481 gcgtctccgg cagccgcgcc agtgcaaaga ccgccagcgt cagcaccggc gcccagtagg
     3541 cccggcccgg ctccggtgcc ggcgccaccg gcggatcgtc gtcggtccgg gccgtgccgc
     3601 ccttgccgcg cagcaccacc gccagcgcca ccagcaccac gacccccggc actgcgctcc
     3661 attcgatgac ctgccgcacc tccgcgtgac gggagaggaa ccaccacgcc gccacggacc
     3721 ccgccaccgc gccgaggtgg tccgcgccac gatggaagcc gaacgcgcgc ccgtgcagtt
     3781 ccgggggcgc gctgtcggcg atgagcgcgt cccgcggcgg cgtccgcagc cccttgccga
     3841 cccggtcgat cacgcggaac ccgatgacct ggcccgccga gttcgcgaag gcgatgagcg
     3901 gccggatgag caccgccgtc ccgtatcccg ccaggatgag cggcttgcgc cagccggggc
     3961 ggtcggagag ccagccgctg gcccacttga ggaccgccgc cgtcaggtcg gcggcgccat
     4021 cgagcgcgct cagcgtgacc gcgcctgccc ccagcaccgt ggtcacgaat gcggggagga
     4081 gcggatagac catctcgctc gcgaagtcgt tgaagagcga gacgagggcc aggccccgca
     4141 ccgtgcgcgg gatccgctcg aacgccctca ttcgtcgagg ccgaggtcca tcccccaccg
     4201 cgccagggag acgggcagcc cgccgtagtc gaggagcgcc tggtggaacg ccttgatcgg
     4261 cgcgtcgacg ccggcgcggg cgcgccaggc atcgtgcagc tggcggatct cccggcgccc
     4321 cgcggcggcg gcgagcccgt aggtgggcca cgccgccact cgccgcacat ccgccaacgc
     4381 ctgcgaccgg tcgagcggca ggtgttccat caggaaggag atcgcctccg ccggcgccat
     4441 cccccgggtg tggagcccga cgtccaccac cacgagcgcg gccgcgtgga gcagcgcgag
     4501 ctggtggaag aactcctcct cggccgactc cgcgaatccg gcacggaaca tcaggtccgt
     4561 ggcatagagc gcccagccat ccaccgacac cggcgtcgac accagccgcc gcacgtcgga
     4621 gcggagcccc gccgagtggg cccggtggag gtggcgcccc gggcagactt cgcggagcgc
     4681 cacgtgcacc agctcttcgc gggccaccgt gggccccggg gtgaggtaca ccaccgtcgg
     4741 cgcttcgtac ccggccaccg gcacgatcgg gcgcaggaac tccggggtgt cgaggagccg
     4801 cacgtcgccc gacggcgcca ccaggaggcc gcgatcgtcg acgaacttcc gggcccgcgc
     4861 caccgtgcgg aggaacacca gctgcgcgtc gcccgcggcg gggcgctccc ggcggaggcg
     4921 ctcggccagc gcccgcggag cagcgccagg ctccacccgt tcggcggcct ggaccaccgc
     4981 ggccgtctgc tcctccagcg accgctggcc ccagcgccag agttcgggtg cgctggcgtg
     5041 gagcgcatgc tcgaagtgga gccgatgctc gaactcatcc tcgccgacgg cgagcgccgc
     5101 cgggtcggcg gtcggcaccg tgcctgacgt gagccgcgcg gcaaagtcgc ggaccgctcc
     5161 ctcggcgagc gccgcggccg ccccgagctg gtcggcgatc cccggcagcc acccggccgc
     5221 cacccgggcc agccgggccg gcaggagcgc caccgcgggc gcgagcgcca ccgcctcgtc
     5281 gacgaacagc accgccggtg ccttgagcgt cgcggcagcc gcagaaagga ccgccggcgc
     5341 tccctggagc cgggccagcg ccgcctgcgc ccgcaattca ggcacccgcc ccgggtgcgc
     5401 cagcaacgac tgccacgcct gcgccacgga gctgagccat gccgccggat tgtgccggtg
     5461 cggcacctcg ctctcgaacc ggaagatcgt ggtgcggaga tcgtcgagga gcccggtgcg
     5521 gtcgatctcc tcctcgtcgt cgagggagag gtcctccacc gcccccgcca gcgagcggag
     5581 cgtagcgagc tggagccgga ccgactcggc gtcgagcatc ggcagcaggg catcggaggc
     5641 ctcgttcccc gcgcgcgtcc cctgcgcagg gtcgaaggtc cagcgcacgt cgaggtacga
     5701 gcggcagagc ccgccgagcc cggacatcag cgccgtcccc cgcgacggtg cacggtcagc
     5761 gccgtccgta ccggtcggtc acgcgcacca ggtcgtcgag ctcgggcgtc gacgcctcga
     5821 ggatgtcgca gtcggtgacc gcctcgacct ggtggaccaa gggcggcggg atgtggagcg
     5881 acgttccggc cgcgacgcgg cgctcggtga gctgcccttc gctccccgag cggaagatca
     5941 tctccccgga caggaggtgc atcgtctcgt ccttccggtg atggtactgc acgctcagcg
     6001 cctgccccgc cttgacgtgc aggatctttc ccacatagcg gtcggtccgc gcccagatga
     6061 gctcgtagcc ccacggcttc tcgaccctgt acggcaggtc catcacgcct ccagcgaacg
     6121 aatggtgaaa gacgacggcg ccgtcacgca cgcccgcaca cataccccgc acccgacgca
     6181 cccctcggcc ttgagcaccg ggtgtccgtc ggcgtccatc gcgagcgcgg aactccccac
     6241 cgggcaggcc tgggcgcaga cgccgcagga ggagccgtgg aacgtgatgc agcgctccgg
     6301 caccagctcg acgaggttga gccggtaccc ggcccacttc ccggccggca ccaccagcgc
     6361 accggtgggg cacgccgcgg cgcagggcat ctcgggacac gccacacacg gctggaggag
     6421 cgggtcgatg aacggcgtcc ccgcggcgag cccggcgtcg gtctcggcgc ggcggatcgc
     6481 cttcggggga cagacgtccg cacacgcgtt gcaccgggtg cacgccgcca ggaacccgac
     6541 ctccggcaac gcccccggcg gccgggcgaa gcgccgctgg accacccggt cctcggtgcg
     6601 cttcaccagc gcgtcgaggt acttgcccac gacgccgcgc aggaagtcgc ggcgctcgcc
     6661 gcccggaggg gtgaacatga ggggaagata cagggaagcg ggcacagcgc gggccaagac
     6721 gggcaaaaag cgggcaaaaa gcgggcgaca agcgggcaag aagcgggcgg aaaggtcggc
     6781 ccgctttccc gcccgctgtc cgcccgctac ttactgacca gcgccctccg gagcgtggcc
     6841 atcttccggc gcaccgagcc gtcgtagcga cgatcgccga cctgcaccac ggccccgccc
     6901 aggatcgtcg cgtcggcctc gaagctcggg atgaccgcct tgccgagagc ccgggtcagc
     6961 gccgcggcga tctccttctg ctgcgccgcg tccgccgcgc gcgccaggac cacccgcgca
     7021 cgcacccgat cgagcttgac gtcgagcagc accaggtact gcgtggcgat ctgccgcagc
     7081 aggcgctgcc gcccgcgctt gaccacggcc accaggaagg cgcggaacgc cttcggcgcc
     7141 ttggggagcg ctgccgtgag gatccgcccc ttctcggcct tggtgacccg cggggacatg
     7201 agcaccgcct cgacccgggg cgccagctcc acggcgcccg ccaccaggtc gatcagctcg
     7261 gcgtactccg ccgccagccc gtcacgctcc gccagggcaa agagcgcctc ggcgtagttc
     7321 cgcgcgacgc cgatcgtgct cacttcgacg gccccagcgt cgccaggtag tcctggacga
     7381 tcttccggtc ctgctccccc tgcaaccgct ggccgatgag cttgcccgcc gccgcgagcg
     7441 ccaggtccac cgcctcgcgc ctgagctcga ccaccgcgcg gtccttctcg gcctcgatct
     7501 cccgccgggc gcgggccatg aggtcgtccg cctctgcgcg ggccttctcg agcccgagct
     7561 gccgctcccg ctccgccgcc gccttggcgt cgttgaggat cgtgacgacc tggccccgcg
     7621 cgtcggcgag cagcttctgc tgctcggcga ggagcgtggc cgccgcctcg cgatccttgg
     7681 ccgcctgggc gatctgctcc tcaagcttct tttcccgggc ctccactgcg cccagcacca
     7741 ccggccacgc gaagcgcttg aggatgagga agaagagccc gaagacgacg agcgtccaca
     7801 ccatcaggcc gccgtccacc gtcaacaggc cgccgccctc cgccatcagc ggcgtggcgc
     7861 cgagcgccgt caggatcccc gccagccaga ccgaccgcat accgacctcg cgtggtgaaa
     7921 tggagcggcg ggggacgctt ccgtcccccg ccccacccgt ccttagaagc tgatcttgcc
     7981 ctgcatgacg aacgcgacga ccgcggcgaa gagcgcgacg ccctcgacca gcgcggcggc
     8041 gatgatcatc gccgtctgga tctgcgccga gttctcgggc tggcgcgcca tgccctcgac
     8101 ggcctggccg ccgatgcggc cgatgccgac gccggcgccg agcaccgcga gaccggcggc
     8161 gaggcccgcg ccgatgaggc cgtagccctt gccgacggac gtgtcagcag cggcctgaag
     8221 caggggaagc atgtgcagca tggtccactc cggttccgtt gacggaagaa cgttggtcag
     8281 gtgcgcccgc cgtggtgccg ccggctcttc cgggaccggc gtcccctggc cgcccgccgg
     8341 tgtcccgaag gacagcgggc ctcgtccgcc gttcggcgga gtacggtgct gcggcggcgg
     8401 ccggggcgcc cggcggccgc gggcctgcta gtgcgacgtc cggatcagcc cgatgaagac
     8461 ggccgtcagg agcgtgaaca cgaacgcgtg caggaacgcc acgaagagct ccagcagcat
     8521 gatccccgtg gccagcacgc tcgcgcccac gccgactgtg tagctgtgga agaagaacgt
     8581 gagcccgagg agcgccagga tcaccacgtg gccggcggtc atgttcgcga acagacgcac
     8641 cgccagggcg aacggcttgg ccagcttccc gatgatctcg atcggcgtca tgatgatgag
     8701 cagcacgccc ttcatgaacg cgccgccgat cccgcccggc aggccgtgcg gcacgaagaa
     8761 gatcgtgccg aggtacccct tggtgccgag cgagcgcatc ccggagatct cgatgatcac
     8821 gagcgccagg atcgccaggg cgccggtcac cgcgatgttg ccggtcgcgg gcgcgccgta
     8881 cggcaggagc ccgagcaggt tactgaacag gatgaagaag aagagggaga gcaggtacgg
     8941 cacgtaccct tcgccatgat gaccgacgtt gggcacgatc acttcctgcc gcacccagag
     9001 cgccatcgcc tcgatggccc ccgcgaaccc ggtggccggc cgccccgccg cctcggcccg
     9061 cgccaccgag cgcgacacca ggaggagcat caggagggcc agcaccgtcg ccagcagcat
     9121 ccagaccagg tgcttggtgg gcgagaagtc gatcgcgagc ggcccgacct gcaccggctt
     9181 ccactgcggc agctcggcct cgcaggtgag ccccggctcg aagcagggat attcgatggc
     9241 gtgggcgttg cccaggtgat gcatgatgtc gatctcgcgg ctcggcacgc cggcgtgctc
     9301 cggcgccgcc ggcccctgcg ccacgacagg cgccgccccg gccccgaatg ccagggccag
     9361 tacgatcgcg atcccgcggg tcaaccggtt catcgtgcct cgatcttgga acgtcccgcc
     9421 gccatcgcca cgaacacctc gacgccaagg agcaccacca gcaccgccgc cagccccacc
     9481 agccccggtc ccatcggcca ccggagcacc ggaaggatca cgaaccctgc cagtgccagc
     9541 agccccaccc gggcacccat ccccacgatc cacgcggtga ggaagcgccc cgtgccgagt
     9601 gtgccgacca gccaccaccc gaggggggcc tgcaccgcca gcgccagcgt gagcccgaac
     9661 caggccccgc gtcccgactc gccggggagc gcgagcgcca ccgcgcccgc ggccgccgcc
     9721 actgccgcca ctccgatcac gtagcgccgg gagtcggatg tcatcgtcac ttcctgccgg
     9781 agcgctcgcg cagctccttg aggaggctcg ccatcatccc cccgaacccg accagcacca
     9841 gcaccaccgt gatgatcccg ccggtcccga accgccggtc aagccactgg ccgagccacg
     9901 ccagggcacc gaccgtcacc gccagttgga tccccaggcc ggcgtatcgg agggcgtcgg
     9961 gcgccctgcc cctgccgggc ggagtgcttg tcacggggcg caaaagtagc cgcttgtgaa
    10021 aagtttcgca aggaaaaaac ggtcgtgagt cgagggtcga gggtcgtggg tctcgggtcg
    10081 aaggtcgcgg gtcacgggtc gtgggtctcg gcgacccagg agcgtcattt tgcggaacaa
    10141 cagttaccat tcatcctgct acccacccgc agcccgcgtc ccctgtcgcg tgtcactctc
    10201 cactcaccgt ctaccccccg ccatgtcccc atccgacgac atcaccgcac tcgcccaact
    10261 cgcccaggcg atccagtcat tgcgggccca ggtcgccgcg cgggtggtgg gccagcggga
    10321 cgcggtcgac gggatgctga ccgcggtgct gagcgggggc cacgcgctcc tggtcggcgt
    10381 gcccggcctc gccaagacgc tgatggtggc gaccctggcc gaggcgctgc agctctcctt
    10441 cagccgggtg cagttcaccc ccgacctgat gccgggcgac atcaccggca ccgacgtgct
    10501 ggaagaggac ccggccagcg gccggcgggt gttccggttc ctgcgcggcc cgatcttcgc
    10561 gaacgtggtc ctggccgacg agatcaaccg caccccgccc aagacccagg ccgccctcct
    10621 gcaggccatg caggaacatc gggtcaccgc gggcgggacc acgtacgacc tgccggcccc
    10681 gttcttcgtc cttgccaccc agaatccgat cgagcaggag gggacctatc cgctcccaga
    10741 ggcgcagctc gaccggttca tgctcgagct tcggatcggc tatcccagtc gggcggaaga
    10801 ggaggcgatc gtcgagcgga ccaccggagc ggccacggcg ccggtgacac cggtcctcga
    10861 cgcagcgacg atcctcgaga tgcaggggct ggtgcgccgc attcccgtgg cccgatcgct
    10921 catcagctcg gcggtcaagc tggcacggat gacccgtccc gacgacgccg atgcgccggc
    10981 ggtg
//
LOCUS       DASUAP010000027        10980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_845659, whole genome shotgun sequence.
ACCESSION   DASUAP010000027 DASUAP010000000
VERSION     DASUAP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845659"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFU45_00690"
     CDS             <1..492
                     /locus_tag="VFU45_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357604.1"
                     /translation="LLLSRREWGRSGDAFPIEITDIRSAHRVLRGADPVGEVEVDPAD
                     LRTALERDLASGLMRFRQAWALHAGEPARLGEVARGAAAPMVALCRGVLTLARNAVPV
                     SADAVVREAARVIGAAPDALATVVAARRQKGWKCPPETFEGFLAAVDAAAQYVDRFSP
                     GDR"
     gene            492..1055
                     /locus_tag="VFU45_00695"
     CDS             492..1055
                     /locus_tag="VFU45_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5357605.1"
                     /translation="MRAMRMTALLSVGALLLSGCGYNRIQQMDEQVNQAKGQIAAQLQ
                     RRADLVPNLVATVKGYAAHEDTVFIHVADARAKLAGAVQGGNIQQMAAANEALTAPLG
                     RLLAISENYPQLKADQNFRALQDELTGTENRIAVARQDYNTAVGDYNKFIRTFPYNLT
                     ARMFGQSTPRDYFQAEGSAAAPPKVQF"
     gene            1060..2157
                     /locus_tag="VFU45_00700"
     CDS             1060..2157
                     /locus_tag="VFU45_00700"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HEU5357606.1"
                     /translation="MTATGQEEFARVRALVLAHGWNSTAYQVVNPGISHWFSSAGDAV
                     TGYVDCGTVWVVAGAPVASEARLPAVVAEFEAAAAQAGASVCYFGAEARLERLCRARA
                     DRAFVTLGAQPCWDPAAWPGILDHHASLRGQLARARNKGVGVTRWASERAQEHPALRA
                     CLTEWLATRGLPPLRFLTTPFTLGRLVDRLVFVAERDGHVVGFLVASPVPARDGWLVE
                     QIVRGAGAPNGTAELLLDAAFTAMIAAGSGYVTLGLAPLSTRGGSGGRIDPWWLRWTL
                     RWARAYGRRFYDFDGLDRFKAKFDPEWWEPVFAMTDQPTFRPRMLYAVAQAFSDNAPI
                     RLVATALVRALRQELRWVVAGHPEGDGTRQG"
     gene            complement(2244..3173)
                     /locus_tag="VFU45_00705"
     CDS             complement(2244..3173)
                     /locus_tag="VFU45_00705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5357607.1"
                     /translation="MDERRWRVVVVPPGAGTSKVLEVSQTVVKLALGVGLVIGLAAVL
                     LSYVTLSRSIAISHNAALARENQLLAEQIGQLQGRMAGLHDTLQTIAERDVRVRLLAN
                     LDPIDPQVAEAGVGGPAPDAPTDSELAVSGVLGRRATDVRVDLDALIRRANLLAWSFN
                     TAAESLSAHSTRLAALPSIMPTRGWLSSAFSQMREHPILHINRPHEGIDVAAPMGAPI
                     EAPAGGVVTSAQWETGYGNVVVIDHGYGIVTKFAHCSKLLVRAGQRVRRGDKVALVGN
                     TGLATGPHLHYEVHVNGKPVDPLRYVLPDAVVD"
     gene            complement(3297..4847)
                     /gene="metG"
                     /locus_tag="VFU45_00710"
     CDS             complement(3297..4847)
                     /gene="metG"
                     /locus_tag="VFU45_00710"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU5357608.1"
                     /translation="MAKFYLTTAIDYANGDPHLGHAFEKIGADAIARYRRLRGDDVWF
                     LIGMDEHGQKVAQTAAERGLSPQALTDQVAVRFQEMWARLGISHDQFMRTTAPQHRDA
                     VRELIERIFRRNPDDFYEKAYAGRYCVGCESFKQDAEIVDGRCALHPTRTLEWVEERN
                     WFFRLSRYQEFLARHIEAHPEFIQPATRRNEILGLLRQGLDDVSASRARFGWGVPFPR
                     PTSDGEAQTTYVWFDALPNYWTATRFPGSRATWPAQLHVIGKDITRFHCVIWPAMLQA
                     AGLPLPERVWAHGFITYGGERFSKSAGVRLELAEAVDRFGADAFRYFLLREVPWDADG
                     SFSWERFEERYTADLADGLGNLASRALAMVEKYRGGVLPTGEATTLDGDGERAVADYQ
                     RAMDVLDLQGGAAAAWSHVAAANLFVQQSAPWTLAKEGRDAELDAVLGALGRSLYRLA
                     ILVAPFMPGKAQALWEALGQAGEVTRIPSPWFRASWGPGKLAWPGGAQVQKPAGLFPK
                     PAPTGSAP"
     gene            complement(4848..5882)
                     /gene="ricT"
                     /locus_tag="VFU45_00715"
     CDS             complement(4848..5882)
                     /gene="ricT"
                     /locus_tag="VFU45_00715"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEU5357609.1"
                     /translation="MTVQLVEVRFKGNRKGYYRWTAEEDPLRLEEKVIVEAERGHDLG
                     RVSAAGESAEKKCGSCAGCERGATPAPSAQPAVVRRATQEDVKALNDLRRTEDEARRK
                     VAERVRHHQLLMKVSDTEWQWDRNKLTIYFTAEKRVDFRALVRDLASQFRTRIDLHQI
                     GVRDEAARLTGVGRCGREYCCSTWLTELSPVNLALAKDQHLSLNPQQISGGCGRLLCC
                     LKYEHEFYVASRMRFPKEGKTVTTAIGAEKVVAVDIFRERVFLRSEQHGPRIITLADL
                     KTEIELLGETLPLAESRPRPSAPAPGPAARPADAAPATAGDRPRRRRRGRRGRGGAGP
                     GAPPPSAPES"
     gene            complement(5879..6841)
                     /locus_tag="VFU45_00720"
     CDS             complement(5879..6841)
                     /locus_tag="VFU45_00720"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU5357610.1"
                     /translation="MAVAYTLEFEKPLQELDRQIDDLKKLGAERSIDVDGEVRVLQGR
                     LEALRREIYASLTPMQRVIVARHPKRPYTLDYLSTIFTDFIELHGDRLYRDDLAIVGG
                     WARLDGTSVMVIGHQKGRDTKENLKRNFGMAHPEGYRKALRLMKLAAKFEAPIITLID
                     TPGAYPGLGAEERGHSEAIARNIIEMASLPTPIIAAVIGEGGSGGALALGVADRVVIL
                     EHSVYSVISPEGCAAILWKDSSQRERAAEALKLTAQDLVQLKVADEIVPEPQGGAHQD
                     PDRMAESLRAVLLRQLKELRSVKPDKLVRRRAEKYASMGAFVEA"
     gene            complement(6844..10323)
                     /gene="dnaE"
                     /locus_tag="VFU45_00725"
     CDS             complement(6844..10323)
                     /gene="dnaE"
                     /locus_tag="VFU45_00725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5357611.1"
                     /translation="MAFVHLHTHSEFSLLDGANRIPELVAHVKKLGMDSLAVTDHGNM
                     HAAWSFYEEARAQKIRPILGFEAYLAFGSRRATQKPADAPAQYSHLVLLAKDRAGYKN
                     LIKLSSIGFTEGFYRRPRIDKEVLAAHSEGVVCLAACLSGEVALYLRQQQYDKARASA
                     EWFARTFGKDDFWLEIQSHGIDEEAIVTEGMLRLGEELGLGVVATNDAHYLRREDAEA
                     HDLLLAIGTGSELDDPKRFRFTGTESYVKSEKEMKALFGDHPDTLANTQVVADRCEFS
                     FEKKYFVPAFPRPEGFVSEEALLEHLALAGITARYGDPIPPAVRERLDYELGVINKAG
                     YAGYFLIVQDFIQAARERGIPVGPGRGSAAGSIVAYALRITDVDPLKFDLLFERFLNP
                     ERVSMPDIDVDFCFERRGEVIEYVRQRYGRGSVGQIITFGTLKARAALKDVARLLRIP
                     PGDADKLTKLVPSGPAFAMTVDEAARKVPELKELVGTDPLYERLFNLSSRIEGISRHA
                     SVHAAGVVIAPGPLADYVPVCTQPTKGAGASQSGEDAIICQYDMIALEKVGMLKMDML
                     GLKTLTVIHDAVEMVAARHGTRPDMDTLPLDDPKVYEMLRQGRTAGIFQFESALATET
                     LRAMKCDRFDDLVASNALLRPGPLDAGMHLVFIRRKLGQEPVSYPHESLREILEPTYG
                     VITYQEQVMRIANVLAGFSLAEADVLRKAVGKKDAALIKKELGKFVERAVAAGHARKM
                     VEELSAQIETFGRYGFNKSHSVAYSVLGYQTAWLKAHYPAEFLAALLSSEIGDTDKVV
                     QYIREAREIGLEVLPPDVNESGFKFTVTGERRIRFGLGAVRNVGQGAIESIIAGRAEA
                     PYRTLQEFVDLIDLRLCNKRVVESLIAAGALDAMGDRAALFAALDSALGEAQLAQLDR
                     ESGQSSLFGDGPAPVRTRAGGLPEVPPWTEAERLTREKEVLGFFISGHPLERWRTEVE
                     LFGTRTTATLHEWSEHTVTIAAVVTVVKRQISKKTGKEYARLVLEDFHGTAEAIVFPD
                     AWAKLNQSITVDQATLLTGGYSPRDRGEERAPFVIETAERLADLRAAGQVGVALRWQA
                     PAAPPPEALRAVAALCAAHPGPSPLYIEWSDGNGERLRLRSKSLRIAPDDALVRALSD
                     VLDRASVHFVKAG"
     gene            10587..>10980
                     /locus_tag="VFU45_00730"
     CDS             10587..>10980
                     /locus_tag="VFU45_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357612.1"
                     /translation="MRTRRSAALLALLAGAVAPALYAQAGRPYPVVLVLPASVRFAGL
                     GGAGVPVIGDAGSVFVNPAGLAAVPKFGVEGAYDRYAGTASQSMAALALRVGQFDFGA
                     GAHYVKFGDTAATRDNLVYVGTAVYRYGL"
BASE COUNT         1516 a         3786 c         3965 g         1713 t
ORIGIN      
        1 ctgctcctca gtcgccggga atggggccgc tcgggcgatg cgttcccgat cgagatcacc
       61 gacattcggt cggcgcaccg cgtgctccgc ggcgccgacc cggtgggtga ggtcgaggtc
      121 gatcccgcgg acctgcgcac cgcgctcgag cgcgatctgg cctcggggct gatgcgattc
      181 cgccaggcgt gggcgctcca cgccggtgag ccggcccggc tcggcgaagt ggcccgcggg
      241 gctgccgccc cgatggtggc gctctgccgc ggcgtgctca cgctggcccg gaacgcggtg
      301 cccgtatcgg cggatgccgt ggtgcgcgag gcggcccggg tcatcggtgc cgcgccggat
      361 gcgctggcca ccgtggtcgc ggcccggcgc cagaagggat ggaagtgccc cccggagacc
      421 ttcgaggggt tcctcgccgc cgtggatgcg gcggcccagt atgtcgaccg tttttccccc
      481 ggagaccggt gatgcgtgcc atgcgaatga ccgccctgct cagcgtcggt gccctcctgc
      541 tctcggggtg cggctacaac cgcatccagc agatggacga gcaggtcaac caggccaagg
      601 gccagatcgc cgcccagctc cagcgccggg ccgacctcgt ccccaacctg gtcgccacgg
      661 tcaagggcta tgccgcccac gaggacacgg tgttcatcca cgtcgctgac gcccgcgcca
      721 agctggccgg cgccgtgcag ggcgggaaca tccagcagat ggcggcggcc aacgaggcgc
      781 tcaccgcgcc gctcggccgg ctcctcgcga tctcggagaa ctacccgcag ctcaaggccg
      841 accagaactt ccgcgcgctg caggacgagc tcaccgggac cgagaaccgc atcgccgtcg
      901 cgcggcagga ctacaacacc gccgtgggcg actacaacaa gttcatccgg accttcccct
      961 acaacctgac cgccaggatg ttcggtcagt cgacgccgcg cgactacttc caggccgagg
     1021 ggtcggccgc ggcgccgccg aaggtgcagt tctgagccga tgaccgcgac cggccaggag
     1081 gagttcgcgc gggtgcgcgc gctcgtcctg gcccacggct ggaattccac cgcctaccag
     1141 gtcgtcaatc ccgggatctc gcactggttc tccagcgcgg gcgacgccgt caccggctac
     1201 gtcgactgcg gcacggtgtg ggtggtggcg ggggcgccgg tcgcgtccga ggcgaggctc
     1261 cccgcggtgg tcgcggagtt cgaggccgct gcggcccagg cgggcgcgtc ggtctgctat
     1321 ttcggcgccg aggcccgcct cgagcggctc tgccgcgcgc gggcggaccg tgccttcgtg
     1381 accctgggcg cccagccgtg ctgggatcct gccgcgtggc cgggcatcct ggaccaccac
     1441 gcctccctgc gcggccagct ggcgcgcgcc cggaacaagg gcgtcggggt gacccgctgg
     1501 gcgtcggagc gggcgcagga gcacccggcg ctccgtgcct gcctcactga atggctcgcc
     1561 acccggggcc tgccgcccct ccgcttcctg accactccct tcacgctcgg acgcctggtc
     1621 gaccgcctgg tattcgtggc cgagcgggat ggccatgtgg tcgggttcct ggtcgcgagc
     1681 ccggtcccgg cgcgggacgg atggctggtg gagcagatcg tgcgcggggc gggggcgccc
     1741 aacggcaccg cggagctcct gctcgacgcg gccttcacgg cgatgatcgc cgcgggctcg
     1801 gggtacgtca cgctcggctt ggcgccgctc tcgacccggg gcggcagcgg cggcaggatc
     1861 gatccctggt ggcttcggtg gacgctgcgg tgggcgcggg cgtacgggag acggttctac
     1921 gacttcgacg gcctcgaccg gttcaaggcc aagttcgacc cggaatggtg ggagccggtc
     1981 ttcgccatga ccgaccagcc gaccttccgg ccgcgcatgc tctacgccgt ggcccaggcg
     2041 ttcagcgaca acgcgccgat ccggctagtg gctacggcac tcgttcgcgc gctgcgacag
     2101 gaactccgct gggtcgtggc ggggcacccg gagggagatg ggacccgcca ggggtgacaa
     2161 tagatggaca acaaaaggaa cggggctccg catcgtgcgg ggccccgtcg ccttctgtcg
     2221 tccgttccag agcgcatgtg ccgtcagtcg accacggcgt cggggagcac ataccgcagc
     2281 ggatcgaccg gcttgccgtt cacgtgcacc tcatagtgca ggtgcgggcc ggtggccagg
     2341 ccggtattgc ccaccagggc caccttgtcg ccgcggcgga cccgctggcc cgcccggacg
     2401 agcagcttgg agcagtgggc gaacttggtg acgatgccgt agccgtggtc gatgaccacc
     2461 acgttgccgt agcccgtctc ccactgcgcg ctggtcacga ccccgccggc cggcgcctcg
     2521 atcggcgcgc ccatcggggc ggccacgtcg atgccctcgt gcggccggtt gatgtgcagg
     2581 atcgggtgct cccgcatctg ggagaatgcg ctgctgagcc agccccgcgt cggcatgatg
     2641 gacggcagcg ccgccaggcg cgtgctgtgc gccgagaggc tctcggctgc ggtgttgaac
     2701 gaccacgcca ggaggttggc ccggcggatc agggcgtcca ggtcgacgcg cacgtccgtc
     2761 gcgcggcggc cgaggacgcc gctgacggcg agctcggaat cggtcggggc atcgggagcg
     2821 ggaccgccca ccccggcctc cgccacctgt ggatcgatcg ggtcgaggtt ggccaggagg
     2881 cgcacccgca cgtcccgttc cgcgatggtc tgcagcgtgt cgtggagccc ggccatccgc
     2941 ccctgcagct gcccgatctg ctcggccagg agctggttct cgcgggccag ggcggcgttg
     3001 tgcgagatgg cgatggagcg cgagagggtg acgtagctga gcagcaccgc ggcgagcccg
     3061 atcacgagcc cgacgcccag cgccagcttg accaccgtct gcgacacctc gaggaccttc
     3121 gaggtgccgg cacccggcgg cacgacgacc acccgccaac gccgttcgtc catccacgct
     3181 cccggaatag gaccggggcc tcagggagag gccccggtgg agaggctaaa gatggagtcg
     3241 ggtaccggac ctgtcaaccc gggactgtaa tctcgaaacc attacgcaac aacgacttac
     3301 ggtgctgacc ccgtgggggc tggcttgggg aagagcccgg cgggcttctg gacctgggcc
     3361 ccccccggcc aggcgagctt gccggggccc cagctcgccc tgaaccaggg tgaggggatc
     3421 cgggtcacct ccccggcctg tcccagcgcc tcccacagcg cctgcgcctt cccgggcatg
     3481 aacggcgcca ccaggatggc caggcggtag agtgaccggc ccagggcgcc gagcacggcg
     3541 tcgagctcag cgtcgcgccc ctccttggcg agcgtccaag gcgctgactg ctggacgaag
     3601 aggttggcgg cggccacatg cgaccaggcc gccgcggcgc ccccctggag gtcgagcaca
     3661 tccatcgcgc gctggtagtc ggcgacggcg cgttcgccgt ccccgtcgag ggtcgtggcc
     3721 tcccctgtcg gcagcacgcc gccccggtac ttctcgacca tcgccagggc gcgcgaggcg
     3781 aggttgccca ggccgtcggc caggtcggcg gtgtagcgct cctcgaaccg ttcccagctg
     3841 aagctcccgt cggcgtccca cggcacttcg cggagcagga agtagcggaa ggcgtcggcg
     3901 ccgaagcggt cgacggcctc ggcgagctcg agccggacgc ccgcgctctt gctgaagcgc
     3961 tcgccgccgt aggtgatgaa cccatgggcc cagacccgct cggggagcgg gagccccgcg
     4021 gcctggagca tcgccggcca gatgacgcag tggaaccggg tgatgtcctt gccgatcacg
     4081 tgcagctgtg ccggccaggt ggcccgggat ccggggaagc gggtggcggt ccagtagttg
     4141 ggcagggcgt cgaaccagac gtaggtggtc tgggcctcgc cgtcactggt ggggcggggg
     4201 aaggggacgc cccagccgaa gcgcgcccgg ctcgccgaga cgtcgtccag tccctggcgc
     4261 aggagcccga ggatctcatt acgtcgcgtg gccggctgga tgaactccgg gtgcgcctcg
     4321 atgtgccgcg ccaggaactc ctggtagcgc gagagccgga agaaccagtt ccgctcctcg
     4381 acccattcga gcgtgcgggt ggggtggagg gcgcagcggc cgtcgacgat ctcggcgtcc
     4441 tgcttgaacg attcgcaccc gacgcagtac cggccggcgt aggccttctc gtagaagtcg
     4501 tccgggttcc ggcggaagat gcgctcgatc agctcgcgca ccgcgtcccg gtgctggggc
     4561 gcggtggtgc gcatgaactg gtcgtgcgaa atgccgagcc gcgcccacat ctcctggaag
     4621 cggaccgcca cctggtcggt gagtgcctgg ggcgagagcc cgcgttcggc cgcggtctgc
     4681 gccaccttct ggccgtgctc gtccatcccg atgaggaacc agacgtcgtc gccccggagg
     4741 cgccggtacc gggcgatggc gtcggcgccg atcttctcga aggcatggcc caggtggggg
     4801 tcgccgttgg cgtaatcgat ggcggtggtg aggtagaact tcgccactca ggactcgggg
     4861 gctgacggtg ggggagctcc gggccctgcg ccgccgcggc cgcgcctccc gcgtcgccgg
     4921 cgccgcggcc ggtcgcccgc ggtggccggg gcagcgtcgg ccggccgcgc agccggaccc
     4981 ggggcgggtg ccgacgggcg cgggcgggat tcggcgagcg gcagcgtctc gccgaggagc
     5041 tcgatctcgg tcttgaggtc cgcgagggtg atgatccggg ggccgtgctg ctcggaacgg
     5101 aggaagaccc gttcccggaa gatgtcgacg gccacgacct tttccgcgcc gatggcggtg
     5161 gtgaccgtct tcccctcctt ggggaagcgc atgcgcgagg cgacgtagaa ctcgtgctcg
     5221 tacttgaggc agcagaggag ccggccgcat ccgccggaga tctgctgcgg gttgagcgag
     5281 aggtgctggt ccttggcgag cgccaggttg accggggaga gctcggtgag ccaggtggag
     5341 cagcagtact cgcgcccgca gcggcccacg ccggtgagcc gcgcggcctc gtcccggacg
     5401 ccgatctggt gcaggtcgat ccgggtgcgg aactgggagg ccaggtcgcg gacgagggcg
     5461 cggaaatcga cgcgcttctc ggccgtgaag tagatggtga gcttgttgcg atcccactgc
     5521 cactcggtgt cgctcacctt catgagcagc tggtggtgcc tgacccgctc ggcgaccttg
     5581 cgacgcgcct cgtcctcggt gcggcggagg tcgttgagcg ccttgacgtc ctcctgggtg
     5641 gcgcggcgga cgacggccgg ctgggccgac ggcgcgggcg tggccccgcg ctcgcacccg
     5701 gcgcaggatc cgcacttctt ctcggcggac tccccggcgg ccgacacccg gccgaggtcg
     5761 tgcccgcgct ccgcctcgac gatgaccttc tcctcgagcc ggagcgggtc ctcctcggcg
     5821 gtccagcggt agtatccctt gcggttgccc ttgaaccgga cctcgacgag ttgcacggtc
     5881 atgcctcgac gaacgcgccc atcgacgcgt acttctccgc gcgccggcgg accagcttgt
     5941 ccggcttgac cgaccggagt tccttgagct gccggaggag caccgcgcgg agcgactcgg
     6001 ccatccggtc ggggtcctga tgggcgccgc cctggggctc gggcacgatc tcgtcggcca
     6061 ccttgagctg caccaggtcc tgggccgtca gcttgagcgc ctcggccgcg cgctcgcgct
     6121 gggaggaatc cttccagagg atggccgcgc acccctcggg ggagatgacc gagtagaccg
     6181 agtgctccag gatgacgacc cggtccgcga cgccgagcgc cagcgcgccg ccggagccgc
     6241 cctcgccgat caccgcggcg atgatgggcg tcgggagcga ggccatctcg atgatgttcc
     6301 gcgcgatcgc ctccgaatgg ccgcgttcct cggccccgag cccggggtag gcgcccgggg
     6361 tgtcgatgag cgtgatgatc ggcgcctcga acttggcggc gagcttcatc agccggagcg
     6421 ccttgcggta tccctcggga tgcgccatgc cgaagttgcg cttgaggttc tccttcgtgt
     6481 cgcgcccctt ctggtggccg atgaccatca ccgacgtgcc gtcgagccgc gcccatccgc
     6541 ccacgatggc gaggtcgtcg cggtagaggc ggtcgccgtg cagctcgatg aaatcggtga
     6601 agatcgtgct gaggtagtcg agggtgtagg gccgcttcgg gtggcgggcc acgatgaccc
     6661 gctgcatcgg ggtcagcgag gcgtagatct cccgccgcag cgcctccagg cgtccctgca
     6721 ggacccgcac ctcgccgtcg acgtcgatgc tccgctcggc gccgagcttc ttgaggtcgt
     6781 cgatctgccg gtccagttcc tggagcggct tctcgaactc gagggtgtac gccacggcca
     6841 tggtcagccc gccttgacga agtggacgga ggcgcgatcg aggacgtcgc tgagggcccg
     6901 gaccagggcg tcatccgggg cgatgcgcag gctcttcgag cgaagccgca ggcgttcgcc
     6961 gttgccatcg ctccattcaa tatagagcgg ggagggcccg gggtgggcgg cgcagagggc
     7021 ggccaccgcc cgcagggcct ccggcggggg ggccgccggt gcctgccacc ggagcgccac
     7081 ccccacctgg cccgcggcgc ggaggtcggc caggcgttcg gccgtctcga tcacgaaagg
     7141 tgcgcgctct tccccgcggt cgcggggact gtacccgccg gtgagaaggg tggcctggtc
     7201 caccgtgatc gattggttga gcttggccca cgcatcgggg aagacgatgg cctcggcggt
     7261 gccgtggaag tcctcgagga ccagccgcgc gtattccttg ccggtcttct tcgagatctg
     7321 gcgcttgacc accgtcacga cggccgcgat cgtcacggtg tgctcgctcc actcgtgcag
     7381 ggtcgcggtg gtgcgggtcc cgaagagctc cacctcggtg cgccagcgct cgagcgggtg
     7441 gccggagatg aagaagccga ggacctcctt ttcccgcgtg aggcgctcgg cctccgtcca
     7501 gggcggcacc tcggggagcc cgcccgctcg ggtgcggacg ggggcggggc cgtcgccgaa
     7561 gagcgaggac tggcccgatt cccggtcgag ctgcgccagc tgggcctcgc cgagggccga
     7621 gtcgagcgcg gcgaagagcg cggcgcggtc gcccatggcg tcgagggcgc cggccgcgat
     7681 gagcgattcc accacccgct tgttgcagag acggaggtcg atcaggtcga cgaattcctg
     7741 cagcgtccgg tacggcgcct cggcgcggcc cgcaatgatg ctttcgatgg cgccctggcc
     7801 cacgttccgg acggcgccca ggccgaagcg gatgcggcgc tcgccggtga cggtgaactt
     7861 gaagcccgac tcgttcacgt cggggggcag cacctcgagc cctatctccc gcgcctcgcg
     7921 gatgtactgc accaccttgt cggtgtcgcc gatctcggag ctcaggagcg cggccaggaa
     7981 ctcggcaggg tagtgcgcct tgagccacgc cgtctggtag cccaggaccg aatatgccac
     8041 cgagtgcgac ttgttgaacc cgtagcggcc gaaggtctcg atctgcgccg agagctcctc
     8101 gaccatcttc cgggcgtggc ctgccgcgac ggcgcgctcg acgaacttgc cgagctcctt
     8161 cttgatgagt gccgcgtcct tcttgcccac cgccttccgg agcacgtcgg cctcggccag
     8221 cgagaacccc gccagcacgt tggcgatgcg catcacctgt tcctggtagg tgatgacccc
     8281 gtaggtcggc tccaggatct cgcgcagcga ctcgtgcgga tagctcaccg gctcctgccc
     8341 gagcttccgg cggatgaaca ccaggtgcat gcccgcgtcg agcggccccg ggcggaggag
     8401 ggcgttgctc gccaccaggt cgtcgaaccg gtcgcatttc atcgcccgca gcgtctcggt
     8461 ggcgagcgcc gactcgaact ggaagatgcc ggcggtccgg ccctgccgga gcatctcgta
     8521 gaccttcggg tcgtcgagcg ggagggtgtc catgtcgggc cgggtgccgt ggcgcgccgc
     8581 caccatctcc accgcgtcgt ggatgacggt cagcgtcttc aggccgagca tgtccatctt
     8641 gagcatgccg accttttcga gtgcgatcat gtcgtactgg cagatgatcg cgtcctcgcc
     8701 gctctgcgag gcgcccgcac ccttggtggg ctgggtgcac acgggcacgt agtcggcgag
     8761 cggccccggc gcgatcacga cacccgcggc gtgcaccgag gcgtgccggc tgatcccctc
     8821 gatccggctc gagaggttga acagccgctc gtagagcggg tccgtgccga ccagctcctt
     8881 gagctccggg accttccgcg ccgcctcgtc gacggtcatc gcgaacgcgg gtcccgaggg
     8941 cacgagcttc gtcagcttgt cggcgtcccc gggggggatc cggaggagcc gcgccacgtc
     9001 cttgagcgcc gcgcgcgcct tgagcgtccc gaaggtgatg atctggccca cgctgccgcg
     9061 gccgtagcgc tggcgcacgt actcgatcac ctcgccccgg cgctcgaagc agaagtcgac
     9121 gtcgatgtcc ggcatcgaca cgcgctccgg attgaggaag cgctcgaaga gcaggtcgaa
     9181 cttgagcggg tcgacgtcgg tgatccggag cgcgtaggcc acgatcgatc cggcggccga
     9241 gccgcggccc gggcccacgg ggatgccccg ctcccgcgcg gcctggatga agtcctgcac
     9301 gatcaggaaa tagccggcat acccggcctt gttgatgacg ccgagctcgt agtcgaggcg
     9361 ctcgcggacc gccggcggga tgggatcgcc gtagcgcgcc gtgatccccg cgagcgccag
     9421 gtgctcgagc agcgcctcct cgctcacgaa cccctcgggg cgggggaagg ccgggacgaa
     9481 gtacttcttc tcgaaggaga attcgcaccg atccgccacg acctgggtgt tggcgagcgt
     9541 gtcgggatgg tcgccgaaga gcgccttcat ctccttctcg ctcttgacgt agctctcggt
     9601 gccggtgaag cggaaccgct tcggatcgtc gagctcgctg ccggtgccga tcgccagcag
     9661 caggtcgtgc gcctcggcgt cctcccgccg caggtagtgc gcgtcgttgg tcgccaccac
     9721 gccgaggccg agctcctccc ccaggcggag catcccctcg gtcacgatcg cttcctcgtc
     9781 gatcccgtgc gactggatct cgagccagaa gtcgtccttc ccgaacgtcc gcgcgaacca
     9841 ctcggcggag gcgcgggcct tgtcgtactg ctgctgccgg aggtagagcg ccacctcgcc
     9901 cgagaggcag gcggcgaggc acacgacgcc ctcgctgtgc gcggcgagga cttccttgtc
     9961 gatccggggg cgccggtaga atccctcggt gaagccgatg gaggagagct tgatgaggtt
    10021 cttgtagccg gcccgatcct tggccagcag caccaggtgg ctgtactgcg ccggagcgtc
    10081 ggcgggtttc tgggtcgcgc gccgggaccc gaacgcgagg taggcctcga agccgaggat
    10141 cggacggatc ttctgggccc tggcctcctc gtagaacgac cacgccgcgt gcatgttgcc
    10201 gtggtcggtc accgccagcg aatccatccc cagcttcttg acgtgcgcca ccagctccgg
    10261 gatgcggttg gcgccgtcga ggagggagaa ttcgctgtgg gtatggaggt ggacgaatgc
    10321 cattgggcta ccagctatca gctatcggct atcagctatc agggatcggt catcggctac
    10381 ggcatcaggg gggcgaagga ccggaccgac gatccaagtg ccggtctttg cccgtcgcat
    10441 tggctgatgg ccgatagctg atagctgata gcttggtgta ccagcggatg attgcgcgtg
    10501 ctcaaggtaa ttccccgtcc ggcgcattgt caaaacccct tgtggcactt acacttggca
    10561 catcgggccg caccgcgccc gcacccatgc gaacccgccg atccgccgcc ctgctggccc
    10621 tcctggccgg cgcggtcgcg cccgccctct atgcccaggc ggggcggccc tatcctgtcg
    10681 ttctggtgct cccggcgtcg gtccggttcg ccggcctcgg cggtgccggc gtgccggtga
    10741 tcggcgatgc cgggtcggta tttgtcaatc ccgccggcct cgccgccgtg ccgaaatttg
    10801 gggtcgaggg cgcctacgac cggtacgccg gcaccgccag ccagtcgatg gcggcgctcg
    10861 cgctccgggt gggacagttc gacttcggcg ccggcgcgca ttacgtcaag ttcggcgaca
    10921 ccgccgccac gcgcgacaac ctggtctatg tcggcacggc ggtgtaccgc tacggactga
//
LOCUS       DASUAP010000028         5258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_137138, whole genome shotgun sequence.
ACCESSION   DASUAP010000028 DASUAP010000000
VERSION     DASUAP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137138"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFU45_00735"
     CDS             <1..232
                     /locus_tag="VFU45_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357613.1"
                     /translation="DNPVVLDVTSGGFLLNGQPVPDAQLEAVLRSIYVPRAAKLLFVR
                     PSSDETLQQVTTAVDRARSAGVEVTALLPAER"
     gene            315..1073
                     /locus_tag="VFU45_00740"
     CDS             315..1073
                     /locus_tag="VFU45_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357614.1"
                     /translation="MGPRAVPLLALQHRAVRSPLGVALSIVLHILVLALLIAPWAYFD
                     PEVRAGLVAALTGGGGGGGRGGDATGVAYISVPAPKAPPPSAPTPVPTPVSRPTVPQV
                     PDATDVAPPVDSAVSPAKGVAAPGQADGAGKGGGTGGGEGPGSGTGSGSATGPGKGGN
                     GGKGTPPDPRQMIIPPNGQVPKALRGRMLAVTFWVTETGDVARVSETPPIEDAKYRRA
                     FEEAMLAYRFRPARDSLGRPTAATIVITQTLGTR"
     gene            1111..2649
                     /locus_tag="VFU45_00745"
     CDS             1111..2649
                     /locus_tag="VFU45_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5357615.1"
                     /translation="MSQLFARKSLADCENDIAERGGLKRSLNKWHLTALGVGATIGAG
                     IFATTGTAIVGDALRPGAGPAIVFSFILTAVTCGFAALCYAEFAAMVPISGSAYTYAY
                     ASLGEIVAWIIGWDLIIEYAVGNIGVAIGWAGYFRELMSHFGVAVPAWLSTDYRSAFD
                     AARQVAAGATDPAMQYLAAAVTGAPHLFGVPVIFNLPAFLVVALITVILVIGIKESAD
                     SNTAMVVLKIGIILFFCAVGATLIKPIHWNDPATGGFAPNGFAGISAGAAIVFFSYIG
                     FDATSTAAEEAKDPARDMPFGIIMSLVVCTVLYIVLAVVMTGMAPWKQLGTPEPMITA
                     LALADGSPRLLSVSRLIVSLGAVIAMSSVLLVFQLGQPRIFMSMARDGLLPPFLSKVH
                     PRFKTPWIGTILTGIFVGVFAAFANIAEVVDLTNIGTLFAFILVSAGVIVLRRTEPDR
                     VRPFRAPWVPVTPIISILACLYLMFQLPTITWIRFGVWLVIGLTIYFSYSVRHSRYRA
                     GHRQ"
     gene            complement(2830..3726)
                     /locus_tag="VFU45_00750"
     CDS             complement(2830..3726)
                     /locus_tag="VFU45_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEU5357616.1"
                     /translation="MTTTFVGVDVGGTKTSAVVGSATEVLSRANGNGAAVRPGRALAS
                     AQIIAEVARKALAASGQLTARAMVVGAAGAGRPAEREELQRALAAEKIADQVHVTTDI
                     ELALVAAFGKEAGIVLSAGTGSIALGRDTTGETHRAGGYGWQMGDEGSGYGIGRAALG
                     AVSRAHDGRGPETKLSIRVMAAAKARTFDQLINWATTATPADVASLAPGVMEMAAHGD
                     AVAQGIVEYAVRELVLLATALAPLTGKAVVPVALSGGLLGEEGPLRSAVTAKLRESAG
                     LNVREEPVEGALGALAMAREMK"
     gene            complement(3723..>5258)
                     /locus_tag="VFU45_00755"
     CDS             complement(3723..>5258)
                     /locus_tag="VFU45_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU5357617.1"
                     /translation="AYRSPLTAYLITEPILHGAPLHSLDLATVVVYLAVITTIGIRAG
                     RGQRTASDYFLGARDLPALAVLFSIVATETSALTVISVPGIGFTGDLTFLQLGFGYVV
                     GRIGVAAWLLPGYFNGSQETAYARLGSRFGDRTRRVVSAVFLVTRFLGDGVRVFAGAI
                     PLALLAGWSIPTAIVVMGAITLLSTWYGGLKAVVWTDVLQLAVYTTGGIVALGIALHL
                     GGGAGAVLSAAAHAGKLRVISAHWNLTEPYTLVGGLVGGALLSAASHGTDHLIVQRLL
                     ATRSLRDARRALVGSGVAVCALFALFLLIGVSIRTGGLAAPDLAGDQVFPQFIIAHLP
                     VGLAGLMIAGILAASMGTHSSAISALASSVTHDLYANWTGRSDPGHLLRIGRAFALGW
                     GVALILAALAFHAFAAGNTPVVVLALSIASVTYGGLLGTYLLAGGWARARGRDVIAGI
                     AVTVVVMTVVIFAARLALLPGLDRLAPVGRLAWPWYVPLGTALTLGVAVLSSFLPHSR
                     AER"
BASE COUNT          768 a         1862 c         1836 g          792 t
ORIGIN      
        1 ggacaatccc gtggtgctcg acgtgaccag cggtgggttc ctgctcaacg gccagccggt
       61 gcccgatgcc cagctcgagg ccgtcctgcg gtccatctac gtcccgcgcg ccgccaagct
      121 cctgttcgtc cgtccctcct ccgacgagac cctccagcag gtgaccaccg cggtcgaccg
      181 ggcccgctcg gccggcgtcg aggtgacggc actccttccc gccgagcggt gatcgcgcgg
      241 gcggcggccg gtgtaggttc ggggggatga cgacgtctcc tcgggagccg gctcctccct
      301 cgtcgaggcc acctgtgggt ccgcgggccg taccgctcct cgccctccag caccgcgccg
      361 tccgctcccc actcggcgtg gcgctctcga tcgtgctcca catcctcgtc ctggcgctct
      421 tgatcgcgcc gtgggcctac ttcgatccgg aggtccgcgc cggcctggtc gcggccctga
      481 cgggcggtgg cggcggcggc gggcgcggtg gcgatgcgac tggcgtcgcc tatatttccg
      541 tgcccgcgcc caaagcccca ccaccgagcg cgccgacgcc ggtcccgacg ccggtttcac
      601 gaccgactgt gccccaagtc cccgacgcga cggatgttgc tccgcccgtc gacagcgcgg
      661 tatcgcctgc gaaaggggtg gcggcgccgg ggcaggctga cggagcgggg aagggcggcg
      721 gtaccggcgg cggcgagggg cccgggagcg ggaccggctc ggggagcgcc accggccccg
      781 ggaagggcgg caacggcggg aagggcaccc cccccgaccc gcgccagatg atcatcccgc
      841 cgaatgggca ggtgccaaag gcgctgcgcg gcaggatgct ggcggtgacg ttctgggtga
      901 ccgagaccgg cgatgtcgcg cgtgtttccg agactccgcc gatcgaggac gcgaagtacc
      961 ggcgcgcctt cgaggaagcg atgctggcct accgcttccg gcccgcgcgc gacagcctcg
     1021 gccgtccgac cgcggcgacc atcgtgatca cgcaaacact cggcactcgg taggcgccct
     1081 gggccggctg gacctttcat cggagagcgc gtgtcccaac tgttcgcccg caaatcgctc
     1141 gcggactgcg agaacgacat cgccgagcgc ggcgggctca agcgctccct caataaatgg
     1201 catcttacgg cgctcggcgt cggcgccacc atcggcgccg gcatcttcgc caccaccggc
     1261 accgccatcg tgggcgatgc gctccgcccc ggcgccggcc ccgccatcgt cttctcgttc
     1321 atcctcaccg ccgtcacctg cggcttcgcg gcgctctgct acgccgaatt tgcggcgatg
     1381 gtgccgatct ccggctccgc ctacacctac gcctacgcct ccctgggcga gatcgtggcg
     1441 tggatcatcg ggtgggacct catcatcgag tacgcggtgg gcaacatcgg cgtggccatc
     1501 ggctgggcgg gctacttccg cgagctgatg agccatttcg gcgtcgcggt ccccgcctgg
     1561 ctctcgaccg actaccgctc cgctttcgac gccgcccggc aagtggccgc gggcgccacc
     1621 gaccccgcca tgcagtacct ggctgccgcg gtcacgggcg ccccgcacct cttcggcgtg
     1681 ccggtcatct tcaacctccc ggccttcctg gtggtggcgc tcatcaccgt catcctggtg
     1741 atcggcatca aggagtcggc cgattccaac acggcgatgg tcgtgctcaa gatcgggatc
     1801 atcctcttct tctgcgcggt cggcgccacc ctgatcaagc cgatccactg gaacgacccg
     1861 gccaccggcg gtttcgcccc gaatggcttt gcgggcatca gcgcgggtgc cgcgatcgtc
     1921 ttcttcagct acatcgggtt cgacgctacc tcgaccgcgg ccgaggaggc caaggacccg
     1981 gcgcgggaca tgccgttcgg catcatcatg agcctggtgg tgtgcacggt gctctatatc
     2041 gtgctggcgg tggtgatgac ggggatggcg ccgtggaagc agctcggcac cccggagccg
     2101 atgatcacgg ccctggcgct cgccgacggc tcgccccggc tcctcagcgt gtcgcgcctg
     2161 atcgtctcgc tcggcgccgt catcgcgatg agcagcgtgc tcctcgtctt ccagctcggc
     2221 cagccccgga tcttcatgtc gatggcgcgg gacgggctcc tgccgccgtt cctcagcaag
     2281 gtccatcccc gcttcaagac cccgtggatc gggacgatcc tcaccggcat cttcgtcggg
     2341 gtgttcgcgg cgttcgccaa catcgccgaa gtggtggacc tcaccaacat cgggaccctc
     2401 ttcgcgttca tcctggtctc ggccggggtc atcgtgctcc gccgcaccga accggaccgg
     2461 gtgcgcccgt tccgcgcccc ctgggtgccg gtgaccccga tcatctcgat cctggcctgc
     2521 ctctacctga tgttccagct gccgacgatc acctggatcc gcttcggcgt ctggctggtc
     2581 atcggcctca cgatctactt ctcctacagc gtacggcact cgcgctaccg ggcggggcac
     2641 aggcagtagg aggggaaaag gaaagagtga aaagggaaaa gtgaaaaggg aatagggaaa
     2701 agggggctcc gtgaccgacg ggcccccgct tcacatcctc acttcgttct tttcacttct
     2761 cacttttcac ttttcacttt tcacttctta cttctcactc cttccttctc atttcttcct
     2821 tctcacttct tacttcatct ccctcgccat cgcgagcgcg ccgagggcgc cttcgaccgg
     2881 ctcctcgcgg acgttgagtc cggcggattc ccggagcttg gcggtcacgg cggagcggag
     2941 cgggccttcc tcgccgagga gtccgccgga gagcgccacc ggcaccaccg ccttgccggt
     3001 gagcggggcg agcgccgtgg cgaggagcac cagctcccgg acggcgtact cgacgatccc
     3061 ctgcgccacg gcgtcgccgt gggctgccat ctccatcacc ccgggggcga gggaggccac
     3121 gtcggcgggg gtggcggtgg tggcccagtt gatgagctgg tcgaaggtac gggccttggc
     3181 ggcggccatc acccggatgg agagcttggt ctccggaccg cggccgtcgt gggcccggct
     3241 caccgcgccg agcgcggcgc ggccgatgcc gtacccggag ccttcgtcgc ccatctgcca
     3301 gccgtaacca ccggcccggt gggtctcgcc ggtggtgtcc cgcccgagcg cgatgctccc
     3361 ggtcccggcg ctcagcacga tccctgcttc ctttccgaac gccgccacca gggcgagttc
     3421 gatgtcggtg gtgacgtgga cctggtcggc gatcttttcc gccgcgagcg cgcgctggag
     3481 ctcctcgcgc tcggcggggc ggccggcgcc ggcggcgccg accaccatgg cgcgtgcggt
     3541 gagctggccc gaagcggcga gcgccttgcg cgccacttcg gcgatgatct gggccgaggc
     3601 gagggcgcgc ccggggcgca cggcagcgcc attgccgttg gcgcggctca gcacctcggt
     3661 cgccgaaccc accacggcgc tggtcttggt gccgccgacg tccactccga cgaaggtcgt
     3721 cgtcatcgtt ccgctctgct gtgggggagg aagctcgaga gcacggccac gccgagcgtg
     3781 agggcggtgc ccaggggaac ataccacggc cacgcgagcc ggcccacggg ggcaagccga
     3841 tccaggccgg gcaggagggc caggcgcgcg gcgaagatga cgacggtcat caccactacg
     3901 gtgaccgcga tgccggcgat gacgtcgcgg ccgcgcgccc gggcccagcc gcccgccagg
     3961 aggtaggtgc cgaggagccc gccgtaggtc accgacgcga tggagagggc cagcaccacg
     4021 acgggggtgt tgcccgcggc gaacgcgtgg aacgccagtg ccgccaggat cagggcgacg
     4081 ccccacccga gcgcgaatgc gcgcccgatg cgcagcagat ggccggggtc ggaacggccg
     4141 gtccagttgg cgtagaggtc gtgggtgacc gagctcgcca acgcgctgat ggccgagctg
     4201 tgcgtgccca tcgatgcggc gaggatcccc gcgatcatga gccccgcgag cccgacgggg
     4261 aggtgcgcaa tgatgaactg ggggaacacc tggtcgccgg cgaggtccgg ggcggcgagc
     4321 ccgccggtgc ggatgctcac gccgatcagc aggaagaggg caaagagcgc gcataccgcg
     4381 acgcccgacc cgaccagcgc ccggcgcgcg tcccggaggg agcgggtggc caggagccgc
     4441 tggacgatca ggtgatcggt gccgtgagaa gcggcggaga ggagggcgcc gccgacgagc
     4501 ccgccaacga gggtgtaggg ctcggtcagg ttccagtgcg ccgagatgac ccggagcttc
     4561 ccggcatggg cggcggcgga gagcaccgcc cccgctcccc cacccagatg gagcgcgatc
     4621 cccagcgcca cgatgccgcc ggtcgtgtac accgccagtt ggaggacatc ggtccacacc
     4681 accgccttga gtccgccgta ccaggtagag agcagcgtga tggcgcccat cacgacgatc
     4741 gcggtgggga tgctccaccc cgcgagcagg gcgagcggga tggccccggc gaacacccgg
     4801 acgccgtcgc ccaggaagcg ggtcaccagg aacaccgccg agacgacccg ccgggtacgg
     4861 tcgccgaagc gcgagccgag gcgcgcgtat gccgtctcct gggagccgtt gaaatagccg
     4921 gggaggagcc acgccgccac gccgatccgc ccgaccacgt atccgaaccc gagctggagg
     4981 aacgtcaggt cgcccgtgaa cccgatcccg gggacggaga tgacggtgag cgccgaggtc
     5041 tcggtggcca cgatcgagaa gagcacggcg agcgccggga ggtcccgggc cccgaggaag
     5101 taatccgacg cggtgcgctg cccccgaccg gcgcggatgc cgatcgtggt gatgacggcg
     5161 aggtagacga cgaccgtggc gaggtcgagg gagtgcaggg gagctccgtg aaggatcggc
     5221 tccgtgataa ggtaagcggt aagcggtgag cggtaagc
//
LOCUS       DASUAP010000029         3896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1213544, whole genome shotgun sequence.
ACCESSION   DASUAP010000029 DASUAP010000000
VERSION     DASUAP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213544"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VFU45_00760"
     CDS             <1..1513
                     /locus_tag="VFU45_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5357618.1"
                     /translation="PRPRPRLVVLIAVDQLRPDYLTRFDRQFTGGFRRILDQGTVFLQ
                     GEQDHAVTVTAPGHSTMLSGRSPASTNILTDELGAPDPAYPILGAPAATGASPMRFRG
                     TALFDWMRAADPGARALSVSRKDRGAIFPIGRARQDVYWYVDGRWTTSTYYRDSLPAW
                     LTAYDARPGARALAGRAWDLLRPDSAYAEPDSEPYEHGGRDFTFPHRLPADSTQAARA
                     VVDFPWMDSLTLDVALEGVRQLKLGRTGHTDLLSISLSTTDAVGHAFGPDSREIHDQV
                     LRVDRWLGQFLDSLSKLVPGDSVVFALTADHGVTPYPEDLRARQQPGGRISLSAIVRQ
                     LDSTYDQRFSVPFGFDEEAGLISGDVSALRARGVDVDSLASAIAAEIRALDGVAQVYT
                     PRSLAAASDADSTAGRWRRELPDDFGWLVAGVAKPGFQWSDGPRSTSHGTTNSPDVLI
                     PIAFWGAGIPAARPTRVARSVDIGPTLAWLVGVTPTERVEGRPLPEVTGVKAR"
     gene            1513..1872
                     /locus_tag="VFU45_00765"
     CDS             1513..1872
                     /locus_tag="VFU45_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357619.1"
                     /translation="MPRYPKLPCFPCPHDAACCAWGTTLSDDEAAAVRREFGRETVYR
                     TRWGEWRTRVRGRRCVFYDRSAGCTLHDRPFYPAVCRGFPWTDAETGGPYEFDQTICP
                     EFITRPELIQIGRRPEP"
     gene            1913..3178
                     /locus_tag="VFU45_00770"
     CDS             1913..3178
                     /locus_tag="VFU45_00770"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5357620.1"
                     /translation="MTRRTVGAMCLLALVSAGAASAQQPTAPDPALAHANRLLSRVPL
                     IDGHNDLPWVIRTDSAAPMSVEAYDIRGHVSGMTDIPRMRAGHLGAQFWSVYTPGEDK
                     DSGYARLQLEQIDIARRMIARYPDALMPAYTADDIERAFKAHKVGSLLGLEGGHAIEN
                     SLGALRAYYDLGVRYMTLTHNVTLDWADAALDSARHGGLTPFGKEVVREMNRLGMLVD
                     LAHVSPGTMSAALDVSEAPVIFSHSGARALVDSPRDVPDSILARVTKNGGVVMVPFYP
                     PFVSPAVHDSEVSYGRQIDSLEAGITDSSQRRTIRQQFRAAHPMPHATIKDVADQIEH
                     IRAVAGVDHVGIGSDFDGIDAWPDSLEDVSTYPRLFAELIRRGWSDADLTKLAGGNVL
                     RVMRQAEAVAARLQRERAPSVRTIRELDR"
     gene            3292..3654
                     /locus_tag="VFU45_00775"
     CDS             3292..3654
                     /locus_tag="VFU45_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU5357621.1"
                     /translation="MAKTFEELSVWQESRALVRDVYRISESGDFRRDLALRVQIRRAV
                     ISIPSNIAEGFERGGGREFRQFVTVAKGSCGEVRTQLYILRDIGHVESDAADPLIERI
                     GRMLGALRRALIPAGRRA"
     gene            complement(3655..>3896)
                     /locus_tag="VFU45_00780"
     CDS             complement(3655..>3896)
                     /locus_tag="VFU45_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357622.1"
                     /translation="LPYPVLPFLLFQADDSTLPRWPRRLDAPTLDEGPYRSYAIQWFA
                     FAAIFLFGGGIWFARRPGTGSADQVPGGEQGPSGG"
BASE COUNT          567 a         1422 c         1324 g          583 t
ORIGIN      
        1 gccgcgcccc cggccccggc tggtggtgct catcgcggtg gaccagctcc ggcccgacta
       61 cctcacccgc ttcgaccgcc agttcaccgg cggcttccga cggatcctcg accaggggac
      121 ggtcttcctg cagggggaac aggaccacgc ggtgacggtc acggcacccg ggcactccac
      181 gatgctctcc ggccgctcac ccgcgagcac caacatcctg accgacgaac tcggcgcccc
      241 ggaccctgca tatccgatcc tcggcgcgcc ggccgccacc ggcgcctcgc cgatgcggtt
      301 ccgcggcacc gcgctcttcg actggatgcg tgccgccgat cccggcgccc gggcgctctc
      361 ggtctcccgc aaggaccggg gcgcgatctt ccccatcggc agggcccggc aggacgtcta
      421 ctggtacgtg gacgggcgct ggaccacgag cacctactac cgtgattcgc ttccggcgtg
      481 gctcaccgcc tacgacgccc gcccgggggc gcgcgccctg gcgggccggg cgtgggacct
      541 gctccgcccc gattcggcgt acgccgagcc cgacagcgag ccgtacgagc acggcggccg
      601 ggacttcacg ttcccgcacc ggctcccggc cgactcgacc caggcggccc gcgcggtggt
      661 cgacttcccg tggatggact ccctgacgct cgacgtggcg ctcgagggcg tccggcagct
      721 caagctcggt cgcaccgggc acaccgacct cttgagcatc tcgctctcga ccaccgacgc
      781 ggtggggcac gccttcgggc ccgattcccg ggagatccac gaccaggtgc tccgggtcga
      841 ccgctggctc ggccagttcc tcgattccct gtcgaagctc gttccggggg acagcgtcgt
      901 cttcgccctc accgccgacc acggggtcac gccatatccc gaggacctcc gcgcccggca
      961 gcagcccggc ggacggatct cgctctcggc catcgtccgg cagctcgact ccacctacga
     1021 tcagcgcttc tcggtgccgt tcggcttcga cgaagaggcc ggcctcatct cgggcgacgt
     1081 gagcgcgctc cgcgcccgcg gtgtcgatgt ggatagcctc gcgagcgcca tcgcggccga
     1141 gatccgcgcc ctggacggcg tggcgcaggt ctacacgccg aggagccttg ccgctgcgag
     1201 cgacgcggac tccaccgcag ggcgctggcg gcgcgaactc cccgacgact tcgggtggct
     1261 ggtggccggc gtggccaaac ccggctttca gtggtcggac ggtcccaggt cgaccagcca
     1321 tggcaccacc aacagccccg acgtgctgat cccgatcgcc ttctggggcg cgggcatccc
     1381 cgccgcgcgt ccgacccggg tggcgcgttc ggtcgacatc gggccgaccc tcgcgtggct
     1441 ggtgggcgtc accccgaccg aacgggtcga agggcgcccg cttcccgagg tgaccggcgt
     1501 caaggcgcgg tgatgccgcg ctatccgaag ctcccgtgct tcccgtgtcc ccacgacgcg
     1561 gcctgctgcg cctgggggac tacgctctcc gacgacgagg cggcggcggt acggcgcgag
     1621 ttcggccggg agaccgtcta tcgcacccgg tggggggagt ggcgcacgcg ggtgcggggt
     1681 cggcgctgcg tcttctacga ccggtcggcg ggctgcacgc ttcacgaccg cccgttctat
     1741 ccggctgtct gccgcggctt cccctggacc gacgccgaga ccggcggacc ctacgaattc
     1801 gaccagacca tctgtccgga gttcatcacc cgcccggagc tcatccagat cggcaggcgc
     1861 ccggagccgt gaaccatcca aggagggacc gctcgtgtcc gtgtcgactt ccatgacccg
     1921 ccgtaccgtg ggggccatgt gcctcctggc actcgtctcc gccggtgccg cgagcgccca
     1981 gcagcccacc gccccggacc cggcgctggc ccacgcgaac cgattgctct cccgggtgcc
     2041 cctgatcgat ggtcacaatg acctcccgtg ggtcatccgc accgactcgg cggcaccgat
     2101 gtcggtcgag gcgtacgaca tccgcgggca cgtaagcggg atgaccgaca tcccccggat
     2161 gcgcgccggg cacctcggcg cccagttctg gtccgtgtac acgccgggcg aggacaagga
     2221 cagcggctac gcccgcctcc agctggagca gatcgacatc gcccgccgca tgatcgcgcg
     2281 ctatcccgac gcgctcatgc ccgcctacac cgcggacgac atcgagcggg ccttcaaggc
     2341 ccacaaggtc ggttcgctcc tgggcctcga aggcgggcac gccatcgaga actcgctcgg
     2401 cgcgctccgg gcctactacg acctcggcgt gcggtacatg acgctgacac acaacgtcac
     2461 gctcgactgg gccgacgccg cgctcgactc cgcccgccac ggcggactca cgcccttcgg
     2521 caaggaagtg gtgcgggaga tgaaccggct ggggatgctg gtggacctgg cgcacgtgtc
     2581 gccggggacg atgagcgcgg cgctcgatgt ctccgaggcg ccggtgatct tctcccactc
     2641 cggggcccgg gccctggtcg actccccgag ggacgtccct gattcgatcc tggcgcgggt
     2701 gacgaagaac gggggcgtgg tgatggtgcc gttctatccg ccgttcgtga gccccgccgt
     2761 ccacgactcc gaggtgagct acggccggca gatcgacagc ctcgaggccg gcatcaccga
     2821 ttcgtcacag cggcgcacga tccggcagca gttccgcgcc gcgcacccga tgcctcatgc
     2881 caccatcaag gacgtggccg accagatcga gcacatccgg gccgtggccg gagtggacca
     2941 cgtcgggatc gggagcgatt tcgacgggat agacgcatgg cccgattcgc tggaggacgt
     3001 ctccacttat ccccggctct tcgctgaact gatccgccgc ggatggagcg acgccgacct
     3061 caccaagctc gccggcggca acgtcctgcg cgtgatgcgc caggcggagg cggtggccgc
     3121 gcggctgcag cgcgaacggg cgccgagcgt gcggacgatc agggaactgg atcggtagca
     3181 gagcccgcga acgaggaacg ggcgggggac gggatcgggt cgcgcgttgc cttgatgttg
     3241 cccgcggcat tttgatgatg gtctggcctc acggcgccgc tatgatcggc aatggcgaag
     3301 accttcgagg aactgtcggt gtggcaggaa tcccgcgccc tggtccggga cgtctaccgg
     3361 atctccgagt ccggggattt tcgtcgggac ctcgcgctgc gcgtgcagat ccgccgggcg
     3421 gtcatttcga tcccatccaa catcgctgaa ggattcgaac gcggcggcgg gcgggagttc
     3481 cggcagttcg tgacggtggc caagggatcg tgtggtgaag tgcgcaccca gctctacatc
     3541 ctgcgtgaca tcggccacgt tgagtcggac gcagcggatc cgctgatcga acggatcggt
     3601 cggatgctcg gtgcgctccg acgagcgctc atcccggccg gccgacgagc ttgatcaccc
     3661 gccactcggc ccttgctccc cacccggcac ctgatccgcg ctccccgtgc ctggtctgcg
     3721 tgcgaaccag atacctccgc cgaacaagaa gatcgccgcg aacgcgaacc actggatcgc
     3781 gtagctcctg tacgggccct cgtcgagggt cggggcgtcg agccggcggg gccagcgggg
     3841 cagggtcgag tcgtcggcct ggaacaggag gaacggcagc accggatagg ggagcc
//
LOCUS       DASUAP010000030         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_832156, whole genome shotgun sequence.
ACCESSION   DASUAP010000030 DASUAP010000000
VERSION     DASUAP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832156"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VFU45_00785"
     CDS             <1..1176
                     /locus_tag="VFU45_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5357623.1"
                     /translation="LVSAPVDDPASANWREEVPHRDDVMVEGFDLFRDFTVVQQRRDG
                     LPELRVMGVDGANDHLIAMSEPVYTATAGTNAEWEAAHYRYSYQSFITPPSVFDYEVR
                     SRTRTLRTQVEVLGGYDPAQYESVRVHAAAADGVQVPVSLVWRRGTPRDGSAPMLLTG
                     YGAYGIPYPVAFSSTRLSLLDRGVVFAVAHVRGGGDLGKPWHDGGRMMNKMNTFTDFI
                     ACAESLLRDGWTSPARLVIEGGSAGGLLMGAVANLRPELWGAVVSHVPFVDVLTTMLD
                     ASLPLTVGEYEEWGDPNDETVYRYLRRYCPWSNLEPKAYPAMYIRTSLNDSQVMYWEP
                     AKYVAKLRALKTDRNPVVLLTNMGAGHGGASGRYDHLHELARDYAWMLQRMGLAGGE"
     gene            1263..1910
                     /locus_tag="VFU45_00790"
     CDS             1263..1910
                     /locus_tag="VFU45_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357624.1"
                     /translation="MPRPTLADLVAALPNFLLAGTAILTWYDPTVLGVAKVSYFVALM
                     LLEFIVLHSAAFMGTVAYGKEPKVGRVAGILGLALFYSLFAGGVALAFHRMWPLWAFW
                     ALVLNRLAGVLMGQGGDDGAAAVVRAGWAAGMMFYLVATLATLFLPLPHLGITAEVVA
                     AQHFTASGLWVEQPWRVLAMAAVYYMLQGVSTLKAHGWAQRKSTNTPLSTNVERG"
     gene            complement(1960..3000)
                     /locus_tag="VFU45_00795"
     CDS             complement(1960..3000)
                     /locus_tag="VFU45_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5357625.1"
                     /translation="MVSAFYWVALSAAIARGPAAGDTVQVGLSEWKVELSAAEATPGR
                     VTFQVKNGGTIPHQFEVEGRGIERRSRLLQPGDSATLVLTLNAGRYEIYCPVGGDSHK
                     KLGMETYLVVGKSRPAARDDDDHAVEAVEGIRVEGAGPVIQILPGPFPFVDSLRSVLP
                     LFGDEAASIRKQAENGPYSDRVARIRGSFAVTAWDLGATRDSLLGLAEFTTADGTRWR
                     LVLDRVQTKDVPHHPRFGGVIFGLYYHGNTGVHTPLVPTINSRVAAWGVAHLFRNDTL
                     VTDDAQVHLMLLSRTRRLGDFALQCWDCSKEPLEELQLQVTPAPGEPAFQAPGGVLFV
                     SWEKSSGKAIRQ"
     gene            3136..3654
                     /locus_tag="VFU45_00800"
     CDS             3136..3654
                     /locus_tag="VFU45_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5357626.1"
                     /translation="METSDHRRFEELILPHLDAGYALARWLLRADEDARDVVQEGALR
                     AVRAFGGYRGGDPRAWFLAIVRNTAWSWRRKHRRDAEWEEFDDELHGNADPARDADLD
                     RLGAVEQVHAALGRLAPEYRDVIVLREFEGLSYREIGEAIGVPVGTVMSRLSRARKRL
                     EELLIRSERSAR"
     gene            3651..4439
                     /locus_tag="VFU45_00805"
     CDS             3651..4439
                     /locus_tag="VFU45_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5357627.1"
                     /translation="MTHDEALAFLDALLDGELAPSEAQEVEAHLSECADCARARERRL
                     ALRERLLHARNAITAPTDLRRQVSDAIAGSGVTRFRPRWRAGAVVRIIGAAAALLLVG
                     TGGWWMAKRGATERELESELLASHIRSLLPGHLTDVLSSDQHTVKPWFDGKVDFSPPV
                     TDLAAEGFPLIGGRLDYVGGRVVAALAYGRRRHLISVFLWPASSPSSASSVPSASPAL
                     LTRQGYHLLRGDCGAYTCWIVSDVGEGDLRELARMLRPSAGSGE"
     gene            complement(4462..4782)
                     /locus_tag="VFU45_00810"
     CDS             complement(4462..4782)
                     /locus_tag="VFU45_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357628.1"
                     /translation="MTFKPAVWKPIALALGAVNLAGVWFAARGGEAWHATLHGALAVA
                     FAVWAQHLRSRSAAGGHELPAGMQTLEGEVNALRQELGEAQERLDFAERLLAQQPERR
                     REKD"
     gene            complement(4796..>4986)
                     /locus_tag="VFU45_00815"
     CDS             complement(4796..>4986)
                     /locus_tag="VFU45_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357629.1"
                     /translation="GIVFVNGHNLGRYWDVIGPQRTLYLPGPWLRKGQNDIAVFEQLN
                     DSVPGHVVGLTAAILDSL"
BASE COUNT          727 a         1777 c         1684 g          798 t
ORIGIN      
        1 ctggtgtcgg ccccggtcga cgaccccgcg agcgccaact ggcgggagga agtgccgcat
       61 cgggacgacg tgatggtcga ggggttcgac ctgttccgcg acttcaccgt cgtccagcag
      121 cggcgcgacg ggctgccgga attgcgcgtg atgggcgtcg acggcgcgaa cgaccacctc
      181 atcgcgatga gcgagccggt ctacaccgcc accgccggga ccaacgcgga atgggaggcg
      241 gcgcactacc ggtacagtta ccagtcgttc atcaccccgc cgtcggtctt cgactacgag
      301 gtccgctccc gcacccgcac cctccggacg caggtcgagg tgctcggcgg ctacgatccg
      361 gcgcagtatg agtcggtccg ggtgcatgcg gcggcggccg acggcgtcca ggtcccggtg
      421 tcgctcgtgt ggcgccgggg caccccccgg gacgggtcgg ccccgatgct cctcaccggg
      481 tatggcgcat acgggatccc gtaccctgtg gcgttctcgt ccacccgcct ctcgctgctc
      541 gaccggggcg tggtgttcgc cgtggcgcat gtccgcggcg gcggcgacct cggcaagccg
      601 tggcacgatg gcggccggat gatgaacaag atgaacacgt tcaccgactt catcgcctgc
      661 gccgagtcgc tgctccggga cggatggacg tcgcccgcgc ggctcgtcat cgagggcggg
      721 agcgcgggcg ggctcctgat gggcgccgtc gccaatctcc gccccgagct ctggggggcg
      781 gtcgtgagcc acgtgccgtt cgtcgacgtc ctcaccacga tgctcgacgc ctcgctcccg
      841 ctcaccgtcg gcgagtacga ggaatggggc gatcccaacg acgagacggt gtaccgctac
      901 ctccggcgct actgcccctg gagcaacctc gaaccgaagg catatccggc gatgtacatc
      961 cgcacctcgc tcaacgacag ccaggtgatg tactgggagc cggccaagta cgtcgccaag
     1021 ctgcgcgcgc tcaagaccga ccggaatccg gtggtgctcc tcaccaacat gggcgccggc
     1081 cacggcggag catccgggcg atacgaccat ctccacgaac tggcccggga ctacgcgtgg
     1141 atgctccagc ggatggggtt ggccggtggg gagtagtcgg tggtcgttgg tcgttagcca
     1201 ctggctgttg gctgttggcc gttgactgat agctgatagc tgatagctga tagctttccc
     1261 ccatgccccg ccccacgctc gccgatctcg tcgccgcgct gcccaacttc ctcctcgcag
     1321 gcaccgccat cctcacctgg tacgacccga ccgtgctcgg cgtcgccaaa gtgagctact
     1381 tcgtggcgct gatgctcctc gagttcatcg tgctgcactc cgccgcgttc atgggcaccg
     1441 tggcctacgg caaggagccg aaggtcgggc gggtcgccgg catcctcggc ctcgcgctct
     1501 tctattcgct ctttgccggc ggggtcgcgc tggcatttca ccggatgtgg cccctctggg
     1561 ccttctgggc cctggtgctc aaccggctcg cgggggtcct gatgggacag ggaggagatg
     1621 atggtgcggc ggcggtggtg cgggcgggtt gggcagcggg gatgatgttc tatctcgtcg
     1681 ctacgcttgc gacgctcttc ctcccgctac cccacctcgg catcacagcc gaggtcgtgg
     1741 cggcccagca cttcaccgca tccgggctct gggtggagca gccctggcga gtgctcgcga
     1801 tggcggcggt gtactacatg cttcagggag tgtcgacgct caaggcgcac gggtgggctc
     1861 agcgcaagtc gacgaacacc cccctctcca cgaacgtgga gagggggtag agggtaggga
     1921 agagcggtgg caacggggac actcgctgaa gagacgcctc tactgcctga tcgccttccc
     1981 gctcgatttc tcccagctga cgaacagcac ccctcccggc gcctggaacg ctggctcccc
     2041 cggagccggc gtcacctgca attgcagttc ctccagcggc tccttcgaac agtcccagca
     2101 ctgcagggcg aagtcaccga gccgccgcgt gcgcgagagg agcatcaggt gcacctgcgc
     2161 atcgtcggtc accagcgtgt cgttccggaa gagatgcgcc accccccacg ccgccacgcg
     2221 gctgttgatc gtcggcacca gcggcgtgtg cacccccgtg ttcccgtggt agtagagccc
     2281 gaagatcacg ccgccgaacc gcgggtggtg cggtacgtcc ttggtctgca cccggtcgag
     2341 tacgagccgc catcgcgtgc cgtcggcggt cgtgaactcg gcgaggccga ggagggagtc
     2401 gcgggtagcg ccgagatccc acgccgtcac cgcgaacgac ccgcggatcc gcgccacccg
     2461 gtcggaatac ggcccgttct cggcctgctt ccggatcgag gccgcctcgt cgccgaagag
     2521 ggggagtacc gaacggaggc tgtccacgaa cgggaacggc cccggcagga tctggatcac
     2581 gggacccgcc ccttccacgc ggatcccctc gaccgcttcc accgcgtgat cgtcgtcatc
     2641 ccgcgccgcc ggccgggact ttccgaccac gaggtacgtt tccatcccca gcttcttgtg
     2701 ggagtcgcct ccgacggggc agtagatctc gtatcgtccg gcattgaggg tcaggaccag
     2761 ggtggcgctg tctcccggct ggaggagccg gctccgccgt tcgatccccc gtccctcgac
     2821 ttcgaactga tgcgggatcg tccccccgtt cttcacctgg aatgtcaccc gtcccggcgt
     2881 cgcctccgcg gccgaaagct ctaccttcca ctccgacagg ccgacctgca cggtgtcgcc
     2941 ggccgccggg ccgcgggcga tggccgcgga cagcgccacc cagtagaatg ccgaaaccat
     3001 atcagaccct ctccgtcaaa tgggacgtga tcgcggacgg aagggtgacg ccgctccggc
     3061 gtcccgtatt cccgggaata accgcgacga tcccggcgtc tgctcagggt gcccgatccg
     3121 aggaatgagg agccgttgga gaccagcgac caccggcggt tcgaggaact gatcctcccc
     3181 catctcgatg cggggtacgc ccttgcgcgc tggctcctcc gggcggacga ggacgcgcgc
     3241 gacgtggtcc aggagggggc gctccgcgcc gtgcgcgcct tcggtggcta ccggggcggg
     3301 gacccgcgcg cctggttcct ggccatcgtg cgcaacaccg cctggagctg gcgccgcaag
     3361 caccgccgcg acgccgagtg ggaggagttc gacgacgagc tccacggcaa cgccgacccc
     3421 gcccgcgatg ccgatctcga ccggctcggc gccgtcgaac aggtgcacgc cgcgctgggg
     3481 cggctggcgc cggagtaccg cgacgtgatc gtcctgcggg agttcgaggg cttgtcgtac
     3541 cgggagatcg gcgaggccat cggcgtcccg gtcgggaccg tgatgtcccg cctgagccgc
     3601 gcccggaagc ggctggaaga gctcctgatc cgctcggaaa ggagtgcccg atgacgcacg
     3661 acgaagcgct cgccttcctc gacgcgctcc tcgatgggga gctcgcgccg tccgaggcgc
     3721 aggaggtcga ggcccacctg tcggagtgcg ccgactgcgc ccgcgcgcgg gagcggcggc
     3781 tggccctccg cgagcggctc ctccacgcgc gcaatgccat caccgcgccc accgacctcc
     3841 ggcggcaggt gagcgatgcc atcgccggga gcggggtgac gcgcttccgg ccccgctggc
     3901 gcgccggtgc cgtcgtgcgg atcatcggcg cggcggcggc gttgttgctg gtgggcactg
     3961 gcgggtggtg gatggccaag cggggcgcga cggagcggga gctcgaatcg gagctcctgg
     4021 caagccacat ccgctcgctc ctgccggggc acctgaccga cgtgctctcg tccgatcagc
     4081 acaccgtgaa gccctggttc gacggcaagg tggacttctc accgccggtg accgacctgg
     4141 cggcggaagg cttcccgctc atcggcgggc ggctcgatta tgtcgggggg agggtcgtcg
     4201 cggcactcgc ctacgggcgg cgccggcacc tgatcagcgt ctttctctgg cctgcttcat
     4261 cgccatcatc cgcctcgtcc gtcccgtccg cctcacccgc cctcctcacc cgccagggct
     4321 accatctcct ccgcggcgac tgcggcgcgt acacctgctg gatcgtgtcg gacgtgggag
     4381 agggcgacct gcgagagttg gcgcggatgc tgaggccatc ggctgggagt ggagaataag
     4441 cgacccttcg cgcgaggcac atcagtcttt ctcccgccgc cgttcgggct gctgcgccag
     4501 aagtcgctcg gcaaagtcca gccgctcctg cgcttcaccc agctcctgcc gcagcgcgtt
     4561 cacctcgccc tcgagcgtct gcatcccggc cggcagttcg tgcccgcccg ccgcactccg
     4621 cgaccggagg tgctgtgccc acacggcgaa cgccacggca agggcgccgt ggagcgtcgc
     4681 gtgccaagcc tcgccgcccc gcgccgcgaa ccacaccccc gccaggttca ccgcacccag
     4741 cgcgagcgcg atcggcttcc acaccgcagg cttgaacgtc acgagcgtct ccgtgtcaca
     4801 atgaatccag gatcgcggcc gtgagcccca ccacgtgtcc cggcaccgag tcgttgagct
     4861 gctcgaagac ggcgatgtcg ttctgcccct tccggagcca cggccccggc agatagagcg
     4921 tccgctgcgg gccgatcacg tcccagtacc gcccaaggtt gtgcccgttc acgaagacga
     4981 tcccct
//
LOCUS       DASUAP010000031         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_368690, whole genome shotgun sequence.
ACCESSION   DASUAP010000031 DASUAP010000000
VERSION     DASUAP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368690"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /gene="tsaB"
                     /locus_tag="VFU45_00820"
     CDS             complement(<1..475)
                     /gene="tsaB"
                     /locus_tag="VFU45_00820"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU5357630.1"
                     /translation="MWLAIDTSADRAAVAVGDAAGPRAERSVQGARQHAAQLLPMIDA
                     VLAEAGAGLGELDGVLLADGPGSFTGLRVGAAVAKGIAAARSLEVRTAPALLILAAGA
                     LPPHAGPVLALTDALRGDVYAAVYDIQEDSVTVLRAPTVVKAAEAASLAPGGAAVV"
     gene            complement(476..886)
                     /gene="tsaE"
                     /locus_tag="VFU45_00825"
     CDS             complement(476..886)
                     /gene="tsaE"
                     /locus_tag="VFU45_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU5357631.1"
                     /translation="MPALTEPELAAFGERLGAELPHGAVVTLAGELGAGKTTLVQAIA
                     RGLGVTAPATSPTYNLVHRYEGRRGPVFHLDCYRLRHPDEAADLDWEGILEEGDAVLI
                     EWPEKAGPWVPAPTRRLVLRHVPGAPDRRDVEAG"
     gene            complement(888..2948)
                     /gene="uvrB"
                     /locus_tag="VFU45_00830"
     CDS             complement(888..2948)
                     /gene="uvrB"
                     /locus_tag="VFU45_00830"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEU5357632.1"
                     /translation="MPPFQVVAPFEPAGDQPKAIGALTDGLRHGEKYQTLLGVTGSGK
                     TMTLAHVIANYGKPTLVLSHNKTLAAQLYGELRQFLPGNAVEYFVSYYDYYQPEAYVP
                     ATDVYIEKDASINQDIEQLRLRATSSLMERNDVVIVATVSAIYGLGDPVQYRKLMVTV
                     TRGESPGRDGILAELVRIQYGRNDVSFEPGTFRVRGDTIEIYPAYADQAVRVELWGDQ
                     VERITKIDPLTGAPIAVLDQAAIYPARHFVTERPTIERAVGLIRAELAERLRVLREAG
                     KLLEAQRLETRTTFDIEMLLEAGMCAGIENYSRHLTGRAEGERPACLIDYFPADFLVV
                     ADESHVTLPQIGGMYNGDRARKTTLVEYGFRLPSALDNRPLRFEEFISLVPQLLAVSA
                     TPGDYELGLSHGHVVEQVIRPTGLVDPEVIIRPVKGQVDDLLGEIRERVQRHERTLVT
                     TLTKRMSEDLTDYLQQTGVRVRYLHSDIDAIERMEILRGLRLGEFDVLVGINLLREGL
                     DLPEVSLVAILDADQEGFLRSDRSLIQTVGRAARNVHGKAILYADRVTGSMQRALDEM
                     DRRRTIQLAYNAEHGITPRSIIKSLEEVKLSTRVADARTERPQAKGKAVPAADLHDPA
                     ERAKAMRALEQQMREAAADLEFEVAAILRDQLNELRALEAPDVRRGAPPQRRGAGRSR
                     AGRR"
BASE COUNT          421 a         1050 c         1109 g          450 t
ORIGIN      
        1 ctaccaccgc cgcgccaccc ggggccagcg acgcggcctc ggccgccttg acgaccgtgg
       61 gcgcccgaag cacggtgacc gaatcttcct ggatgtcgta gaccgcggcg tagacgtcgc
      121 cgcgaagggc gtcggtgagc gccagcaccg ggcccgcgtg cggcggcagc gcccctgccg
      181 cgaggatcag gagcgcgggc gcggtccgca cctccagact ccgcgccgca gcgatcccct
      241 tggcgaccgc ggcccccacc cgcaatcccg tgaagctccc cggcccgtcg gcgagcagca
      301 cgccgtcaag ttcgcccagg ccggcgcccg cctcggccag caccgcgtcg atcatcggca
      361 gcagctgcgc ggcgtgctgc cgcgccccct gcaccgaccg ttcggcccgg ggccccgcgg
      421 catcacccac ggcgacggcg gcccggtcgg ccgaggtgtc gatcgcaagc cacatctagc
      481 cggcctccac gtcgcgacgg tccggggcgc cgggcacatg ccggagcacc aggcgccggg
      541 tcggcgccgg cacccaggga cccgccttct ccggccattc gatgagcacc gcgtcccctt
      601 cctccaggat cccttcccag tccaggtcgg cggcttcgtc gggatggcgc agccggtagc
      661 agtcgaggtg gaacaccggc ccgcgccggc cctcgtagcg atgcacgagg ttgtaggtgg
      721 ggctcgtggc cggcgcggtg acgccgagcc cgcgggcgat ggcctgcacc agggtggtct
      781 ttcccgcgcc gagctcaccg gcgagcgtca ccacggcgcc gtgggggagc tcggcgccga
      841 gccgctcgcc gaaggccgcg agctcaggct ccgtcaacgc aggcatccta gcgccgtccg
      901 gcccgcgagc ggccggcacc ccgccgctgc ggcggggcgc cgcgacgcac gtcgggcgcc
      961 tcgagggcgc ggagttcgtt gagctggtcg cgcaggatgg cggccacctc gaactcgagg
     1021 tcggccgccg cctcgcgcat ctgctgctcg agcgcccgca tggccttggc ccgctccgcc
     1081 ggatcgtgga ggtcggcggc gggcaccgcc ttccccttgg cctgcgggcg ctcggtccgg
     1141 gcgtcggcca cgcgggtgga gagcttgacc tcctccagcg acttgatgat cgagcggggc
     1201 gtgatgccgt gctcggcgtt gtaggccagc tggatcgtcc gccgccggtc catttcgtcg
     1261 agcgcccgct gcatcgagcc ggtgacccgg tcggcgtaga ggatcgcctt gccgtggacg
     1321 ttgcgcgccg cgcggcccac cgtctggatg agagagcggt cggagcggag gaacccctcc
     1381 tggtcggcgt cgaggatggc gaccagcgac acttcgggca ggtcgagccc ctcgcgcagc
     1441 aggttgatgc ccaccagcac gtcgaactcg ccgagccgga gcccgcgcag gatctccatc
     1501 cgctcgatcg cgtcgatgtc ggagtggagg tatcgcaccc gcaccccggt ctgctgcagg
     1561 tagtcggtca ggtcctccga catccgcttg gtgagcgtgg tgacgagggt gcgctcgtgg
     1621 cgctggaccc gctcgcggat ctcgcccagc aggtcgtcga cctggccctt cacgggccgg
     1681 atgatgacct cgggatccac cagcccggtg gggcggatga cctgctccac cacgtggccg
     1741 tgggagaggc cgagctcgta gtcgcccggc gtggccgaga cggcgaggag ctggggcacc
     1801 agcgagatga actcctcgaa tcggagcggc cggttgtcga gcgccgaggg gagccggaag
     1861 ccgtactcga cgagggtcgt tttccgcgcg cggtcgccgt tgtacatccc gccgatctgc
     1921 ggcagcgtca cgtggctctc gtcggccacg acgaggaagt cggccgggaa gtagtcgatg
     1981 aggcaggcgg ggcgttcgcc ttcggcgcga ccggtcaggt gccgggagta gttctcgatg
     2041 ccggcgcaca tcccggcctc gaggagcatc tcgatgtcga aggtggtgcg ggtctccagg
     2101 cgctgcgcct cgaggagctt tcccgcctcg cgcaggaccc gcagccgctc ggccagctcg
     2161 gcgcggatga gccctaccgc gcgctcgatc gtcgggcgct cggtgacgaa gtgccgggcg
     2221 ggatagatgg ccgcctggtc gagcaccgcg atcggtgccc cggtgagcgg gtcgatcttg
     2281 gtgatccgct ccacctggtc gccccagagc tcgacccgca ccgcctggtc ggcgtacgcg
     2341 ggatagatct cgatcgtgtc accccggacc cggaaggtgc cgggctcgaa gctcacgtca
     2401 ttgcggccgt actggatccg caccagctcc gccaggatcc cgtcgcggcc gggactctcc
     2461 ccccgggtga cggtgaccat gagcttccgg tactgcaccg ggtcgcccag gccatagatg
     2521 gcgctcaccg tggccacgat gaccacgtcg ttccgctcca tcaacgacga cgtggcccgc
     2581 aggcggagct gctcgatgtc ctggttgatc gaggcgtcct tctcgatgta tacgtcggtc
     2641 gccgggacgt aggcctcggg ctggtagtag tcgtagtagg agacgaaata ctcgaccgcg
     2701 ttgccgggca ggaactgccg gagctcgccg tagagctggg ccgcgagcgt cttgttgtgc
     2761 gacagcacca gcgtcggctt gccgtagttg gcgatgacgt gcgccagcgt catcgtcttc
     2821 cccgagccgg tgacgccgag gagcgtctgg tacttctccc cgtgacggag gccatcggtg
     2881 agcgcgccga tggccttggg ctggtcaccg gcgggctcga agggggcgac gacctggaac
     2941 gggggcatgg cgggagtata gcactatcgg tgtttgttcg ggagagggga gaggagaagg
     3001 aggagaagag gaggagaaat ggagaaggag
//
LOCUS       DASUAP010000032         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_968412, whole genome shotgun sequence.
ACCESSION   DASUAP010000032 DASUAP010000000
VERSION     DASUAP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968412"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFU45_00835"
     CDS             <1..827
                     /locus_tag="VFU45_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEU5357633.1"
                     /translation="SAFPDQAFTAGVERIREYIRAGDVFQAVLSRRLDLPAPDPFLAY
                     RYLRALNPAPYLFFLQCDDLHLVGSSPEVLVRVEGDEVTVRPIAGTRPRGATAEADAR
                     NEEALLADPKEVAEHLMLLDLGRNDVGRVAAYGSVRVTAQMVIERYSHVMHIVSEVRG
                     RLRAGIDALAALAAAFPAGTVSGAPKVRAMEIIDELEPTRRGPYAGAVGYVGWGARSL
                     DTAIAIRTCVIRGGRAWVQAGAGIVADSDPALELAETEAKARGVLAALALAARDRH"
     gene            864..2462
                     /locus_tag="VFU45_00840"
     CDS             864..2462
                     /locus_tag="VFU45_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5357634.1"
                     /translation="MTTFRLVAVTSDQSFDCPPGRVFVLGRATGNDLAVPDPTISRRH
                     AEIEATPAGLHIKDLGSSNGTFINGAKITEGVATTKDTVTFGKVTFAVQSVSAVASAA
                     GPVLSPMPGGTIVRPIASLSGSGAALANPDGSPSKVGLLRVQGTNTQERQAKKLSLLL
                     DIAQKLSGQLDLDRVLGTVADMTFEVMAVDRVSILLRNEVTGELNSRVARSRLGDASS
                     HAVPRSIANKAVEEKVAILTDNAAADARFKGQSIVLQSVRSAMCSPLIDTKDEAIGLL
                     YVDNMTATNSFSDEDLQFLVAFSNLASIAVRNVRFAEQIQREAMVRSNFERYFAPNVA
                     AEIAMQADAVKLGGEKRPVTVMFTDIRGFTSMSEAMNPDAIAQLLSDYFTEMVDIIFE
                     FGGTLDKFIGDAIMALWGAPIAHPDDPDRAVQAAIAMQHAMAELNERLKKEGRPRIGV
                     GIGINHGEVFAGNIGSHRRLEYTVIGDAVNTASRLCSKAAGGEILISQPFFDALSDKP
                     PVDQLEPLALKGKTQAVPVLRVKA"
     gene            complement(2667..>2943)
                     /locus_tag="VFU45_00845"
     CDS             complement(2667..>2943)
                     /locus_tag="VFU45_00845"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEU5357635.1"
                     /translation="VITSIALLAGGRLHQATDVTKVTFRAAGDEYLRAYIATGEPMDK
                     AGAYGIQGYGAALVERVEGDFFSVMGLPVRLVLKVLAEAGWEYRFGG"
BASE COUNT          467 a         1092 c          961 g          423 t
ORIGIN      
        1 tctcggcctt ccccgaccag gcgttcacgg ccggggtgga gcggatccgc gagtacatcc
       61 gggcgggcga cgtattccag gcggtgctgt cccgccggct cgacctcccg gccccggatc
      121 cgttcctcgc gtatcgctac ctgcgcgcac tcaacccggc gccatacctg ttcttcctcc
      181 agtgcgacga cctgcacctc gtggggagct cgcccgaggt gctggtgcgg gtcgaggggg
      241 acgaggtgac cgtccgtccg atcgcgggga cccgcccccg gggtgccacc gcggaagcgg
      301 acgcgcggaa cgaggaggcg ctcctggccg accccaagga ggtggcggag cacctgatgc
      361 tcctcgacct cggtcgcaac gacgtgggac gggtggcggc gtacggcagc gtccgggtca
      421 ccgcgcagat ggtcatcgag cggtactccc atgtgatgca catcgtgagc gaggtgcggg
      481 ggcggctccg cgcggggatc gacgcgctgg ccgcgctcgc cgcagcgttc ccggccggca
      541 ccgtgagcgg cgcccccaag gtccgcgcga tggagatcat cgacgaactg gagcccaccc
      601 gccgcggccc gtacgccggc gcggtggggt acgtgggctg gggcgcgcgg agcctcgaca
      661 ccgccatcgc catccgcacc tgcgtcatcc ggggcggccg cgcctgggtc caggcggggg
      721 ccgggatcgt ggccgactcc gaccccgccc tggagctcgc cgagaccgag gccaaggccc
      781 gtggggtgct cgccgccctc gcactcgcgg cccgggaccg ccattagcgc gccccgccgg
      841 ccgccggtat atttcgccct ccgatgacca ccttccgcct cgtcgccgtc acgtccgacc
      901 agagcttcga ttgccccccc ggccgcgtct tcgtgctcgg ccgcgccacg ggcaacgatc
      961 ttgccgtccc cgacccgacc atctcccggc gccacgccga gatcgaggcg acgccggcgg
     1021 gcctccacat caaggacctc ggcagctcca acggcacgtt catcaacggc gccaagatca
     1081 ccgagggggt cgccaccacc aaggacacgg tgaccttcgg caaggtgacc ttcgcggtgc
     1141 agtccgtgtc ggcggtcgcg tcggccgcgg gcccggtgct ctcgccgatg ccgggcggca
     1201 cgatcgtccg gccgatcgcg agcttgagcg ggagcggcgc agcgctcgcc aaccccgacg
     1261 gctcgccgtc gaaggtcggc ctcctccggg tccagggcac caacacccag gagcgccagg
     1321 ccaagaagct ctcgctcctc ctcgacatcg cccagaagct ttccggccag ctcgacctcg
     1381 accgggtgct cggcaccgtg gccgacatga ccttcgaggt gatggcggtg gaccgggtgt
     1441 cgatcctgct ccgcaacgag gtgaccggcg agctcaactc gcgggtggcg cggagccgcc
     1501 tcggcgatgc ctcgagccac gccgtgccgc gctcgatcgc caacaaggcg gtggaggaaa
     1561 aggtcgccat cctgaccgac aacgcggccg ccgatgcccg gttcaaggga cagtcgatcg
     1621 tgctccagag cgtccggagc gccatgtgca gcccgctcat cgacaccaag gacgaggcga
     1681 tcgggctcct ctacgtcgac aacatgaccg ccaccaactc gttcagcgac gaggacctgc
     1741 agttcctggt ggcgttcagc aacctcgcgt cgatcgcggt gcgcaacgtg cggttcgccg
     1801 agcagatcca gcgcgaggcg atggtccggt cgaacttcga gcggtatttc gcccccaacg
     1861 tcgccgccga gatcgcgatg caggccgacg cggtcaagct ggggggcgaa aagcgcccgg
     1921 tgacggtcat gttcaccgac atccgcggct tcacctcgat gtccgaggcg atgaaccccg
     1981 acgccatcgc ccagctcctc tcggactact tcaccgagat ggtcgacatc atcttcgagt
     2041 tcggcggcac gctcgacaag ttcatcggcg atgcgatcat ggccctctgg ggcgcgccga
     2101 tcgcccaccc ggacgacccc gaccgcgccg ttcaggccgc catcgcgatg cagcacgcga
     2161 tggccgagct caacgagcgc ctgaagaagg aggggcgccc gcggatcggc gtcgggatcg
     2221 ggatcaacca cggcgaggtc ttcgcgggga acatcgggag ccaccgccgg ctcgagtaca
     2281 ccgtcatcgg cgacgccgtc aacaccgcca gccggctctg ctccaaggcc gccggcggcg
     2341 agatcctgat cagccagccc ttcttcgacg ccctctcgga caagcccccc gtcgaccagc
     2401 tcgagccgct ggccctcaag ggcaagacgc aggcggtgcc ggtgctcaga gtgaaagcgt
     2461 aggaaagcgg ggaaggcgga gaagaggttc aggatccggt tcaaggtaca gggcaaaggc
     2521 tggcgagcag gcgagaaaga agaagagggg cttcccgggt cactgggagc cccttgttcg
     2581 cgccttctcg cattcccgcc cacttgctcc ctgccccctg cacccctccg ataccaactt
     2641 gcaccttccc ccgttttccc ccggctttat cctccgaacc gatactccca ccccgcctcc
     2701 gccagcacct tgagcaccag ccgcaccggc agccccatca cgctgaagaa atctccctcc
     2761 acccgttcca ccagcgccgc gccatagccc tggatcccat acgcgccggc cttgtccatc
     2821 ggctccccgg tcgcgatgta ggcccgcaga tattcgtcgc cggcggcgcg gaacgtcacc
     2881 ttcgtgacat cggtggcctg gtggagcctg cccccggcca ggagcgcgat cgacgtgatg
     2941 acc
//
LOCUS       DASUAP010000033         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_477757, whole genome shotgun sequence.
ACCESSION   DASUAP010000033 DASUAP010000000
VERSION     DASUAP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477757"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFU45_00850"
     CDS             complement(<1..414)
                     /locus_tag="VFU45_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357636.1"
                     /translation="MLMTKRAHVGMALTLAAALGLAACGSKGSTGPSFAATISDSAAA
                     AAGQSGADFAGTMMGTFDFGGPGINLSAQSAPKAVALLNGAWMYATHSSKPRYTVKGL
                     ATAPVMQFSSMSGCNGGNYTTVSGDTTDTDNDGIPA"
     gene            753..>2726
                     /locus_tag="VFU45_00855"
     CDS             753..>2726
                     /locus_tag="VFU45_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5357637.1"
                     /translation="MSIPHQLRCLGYPTLHAPDGSAVRFRVKKHLALLVYLAMAPRIH
                     RRDRLAELLWPAVSEREGRHSLATGLSVLRAKLGHAAIEAGRDTVRLVPGSLELDLDR
                     LAAGDILGSETVPVLAVAPLLEDFDIPDAPGWNQWKDAEAANWQPRIRDALLTLTDRS
                     RRHGDTRAMERLADSLLAIDPLSEEGIRSKMEARAFAGDRLSALKHFEAWKARLADEV
                     GAQPGELVEGMAVRLRRRGWERTNESHIAPVHTDQWKDRPFIGRGAQYRVLYEAWEST
                     ERGAPRHAIITGDSGVGKSTLAERLATAAGLQGAVSAREQCHEVARDVPYAAIGGLAV
                     GLLDKPGASTIAPEWLGELGRAVSEVRRRFPHAPPPLEAEGETVRIRIADAFVELVNA
                     VAEEQPVILILDDLHLADDASVSVLHLLLRRLERQRVQVLLTARPGEISNGGNAQRLV
                     ERADQVGLSGVAVPPLSPEEASELLDSLVATRGEAGPTPAARRALLQAAAGYPMVLEL
                     LTDDWHTHGERCLALSLGGMTEQAATEGPGAMYARILERLTAELDPTSHNVLNLAAVL
                     GARLNDFALYGLADLTLAQTMAGLSRLAAVRILRDAGEGLEFINELMRAQVYVAVPSS
                     VRRVLHSKVADQLIARADGGEPLLGLEIAWHCIR"
BASE COUNT          418 a          932 c          972 g          404 t
ORIGIN      
        1 ggccgggatc ccgtcgttat ccgtatcggt ggtgtcaccc gagacggttg tgtagttgcc
       61 gccattgcac ccgctcatgc tggagaactg cataaccggg gcggtggcaa ggcccttgac
      121 ggtgtagcgc ggcttggatg aatgcgtggc gtacatccag gccccgttga gcagggccac
      181 cgccttgggg gccgactggg cagagaggtt aatgcccgga ccgccgaagt cgaacgtgcc
      241 catcatcgtg ccggcaaagt cggcacccga ctggcccgcg gccgcggccg cggaatcgga
      301 gatggtggcg gcgaagctcg gcccggtcga tcccttgctg ccgcaggcgg cgagcccgag
      361 cgcggcggcg agggtgaggg ccatcccgac atgagcccgc ttggtcatga gcactgctcc
      421 tcgcgctgct ggtgaaggtg gctggcgcgg aaggtcgcca gcgggagcta ttgtgggtcg
      481 cgggaagcga ctccgtggag gtacggataa gacggagcaa agatgcaaca aatccccggc
      541 gccgccagtg ccctccgtca cattcgtgaa aatggcttcc atgcaaggag ttaccgcatc
      601 ctgcgccact agggaaaacc ctagtgggcg cccttaatcc ccgatactgg gaaggggatc
      661 ggctccgcgc acaggccgca gtggtcgcag cgatttgacg ttcggttgct ggcgccggtg
      721 gagtctttgt aacatcttaa taccggctcc ctatgtccat accccaccag ctccgctgtc
      781 tcggctaccc gaccctccac gcgcccgacg gctccgccgt ccggttccgg gtcaagaagc
      841 atctggcgct cctcgtctat ctggcgatgg cgccgcggat ccaccggcgc gaccggctgg
      901 ccgagctcct ctggccggcg gtgagcgaac gggaggggcg gcattccctg gccaccgggc
      961 tctccgtcct ccgcgccaag ctggggcacg cggccatcga ggccgggcgg gacaccgtgc
     1021 ggctcgtccc cggctcgctg gaactcgacc tcgaccgcct ggccgcgggc gacatcctcg
     1081 gcagcgagac cgtgccggtc ctcgcggtgg cgccactcct cgaggatttc gacatccccg
     1141 atgcgccggg gtggaaccaa tggaaggacg ccgaggcggc caactggcag ccccggatcc
     1201 gcgacgcgct gctgactctt accgaccgct cccgccgcca cggcgacacg cgcgcgatgg
     1261 agcgcctggc cgattccctc ctcgccatcg atccactgag cgaggagggg atccggtcca
     1321 agatggaggc ccgcgcattc gccggcgacc ggctgagcgc cctcaagcat ttcgaggcgt
     1381 ggaaggcccg gctggccgac gaggtcggcg cccaacccgg tgagctggtc gaggggatgg
     1441 cggtgcggct ccggcgccgg ggctgggagc ggacgaacga gtcccacatc gcgccggtac
     1501 ataccgacca gtggaaggac cgcccgttca tcgggcgggg ggcgcagtac cgggtgttgt
     1561 acgaggcctg ggaatcgacc gaacgcgggg cgccgaggca cgccatcatc accggcgatt
     1621 ccggcgtcgg gaagagcacg ctcgccgagc ggctggcgac ggcggcgggg ttgcagggtg
     1681 cggtgagcgc ccgggagcag tgccatgagg tcgcgcgcga cgttccctat gccgccatcg
     1741 gcgggctcgc cgtgggactc ctcgacaagc caggcgccag cacgatcgcg cccgaatggc
     1801 tcggcgaact ggggcgtgcc gtgagcgagg tgcggcggcg gtttccccac gcgccgccgc
     1861 cgctcgaagc ggaaggtgag accgtccgga tccgcatcgc cgacgcgttc gtcgagctcg
     1921 tgaacgcggt cgccgaagag cagcccgtca tcctgatcct cgacgacctc cacctggcgg
     1981 atgacgcgag tgtctccgtg ctccacctgc tgctgcggcg gctcgaacgc cagcgggtgc
     2041 aggtgctcct caccgcgcga ccgggcgaga tctcgaacgg cggcaacgcc cagcgcctgg
     2101 tcgagcgggc cgaccaggtg ggcctgagcg gcgtcgcggt gccgcccctc tcccccgaag
     2161 aggcctcaga gctgctcgac tccctggtgg ccacccgcgg cgaagccgga cccacgccgg
     2221 cggcgcgacg ggcgttgctc caggccgccg ccgggtatcc gatggtcctg gagctcctca
     2281 ccgacgactg gcacacccac ggggagcggt gcctggcgct gagcctgggg ggcatgaccg
     2341 agcaggccgc caccgaaggc ccaggcgcca tgtacgcgcg gatcctggaa cgcctgaccg
     2401 ctgagctcga tccgaccagc cacaacgtgc tcaacctcgc ggccgtcctc ggcgcccggc
     2461 tcaacgactt tgcgctttat gggcttgccg acctgacgct ggcacagacg atggccggac
     2521 tgtcccgcct ggcggcggtg cgcatcctgc gggacgccgg tgagggactc gagttcatca
     2581 acgagctgat gcgggcgcag gtgtacgtag cggtgccgtc atcggtccgg cgggtgctgc
     2641 acagcaaggt ggccgaccag ctcatcgcgc gggctgatgg cggcgagccg ttgctcggcc
     2701 tggagatcgc atggcattgc atccgc
//
LOCUS       DASUAP010000034         6734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_219027, whole genome shotgun sequence.
ACCESSION   DASUAP010000034 DASUAP010000000
VERSION     DASUAP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219027"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..803)
                     /locus_tag="VFU45_00860"
     CDS             complement(111..803)
                     /locus_tag="VFU45_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU5357638.1"
                     /translation="MPRASVIAHRGASGYAYQNSIAAFALAREMGADGIELDIHATTD
                     GALVVHHDPEVEGLGIIAEHTFADVQRVTLPNGEPIPTLDAALAAAGALDVWIEVKTL
                     PARWDDRLLETIARGPAPSRYGVHAFDHRIIARLGARRPGLRLGVLSASRPIDPVAQV
                     SAAGAGVLWQESSLTDADLVAALHAAGKGVIVWTANTPEELARLSHLGVDGLCGNFPD
                     RTRAAVGLSPRP"
     gene            876..1424
                     /locus_tag="VFU45_00865"
     CDS             876..1424
                     /locus_tag="VFU45_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5357639.1"
                     /translation="MTPAPESGAPEDVDLVAGWRAGDQAAATALVARHTGAVARFLHS
                     SGAPPADLDDLVQEAFFRAFRRIDSWRGDAGFRAWLISIAGNLLRDQWRRRKGRRILP
                     LDDRDLAGADAPDGEVEADEAAERIRAELGRLPRLQREVFLLRVQEGAGYEEIARALG
                     TTSGAARVHYHHAVRRLKERVR"
     gene            1421..1927
                     /locus_tag="VFU45_00870"
     CDS             1421..1927
                     /locus_tag="VFU45_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357640.1"
                     /translation="MTSCEGLQERMPAVARDEDRWSAADLAHVRGCAECSAEWSVVEG
                     TVRLGHATPAVDPGRTAAAVVRRLGADRVRARVRRRWVGGAVGVAAAAALAFVLWPAA
                     RPAPAAPVELQASVFLPELDSLTVDQLKAVLGAVDRPLGSDRTLDSSGLSGLSDDELS
                     AVLQSMEG"
     gene            1927..2427
                     /locus_tag="VFU45_00875"
     CDS             1927..2427
                     /locus_tag="VFU45_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357641.1"
                     /translation="MRFLGRSLAAIALIAAPLTAQRVPPDQRGVLQRRIENIFLARVT
                     EELGLTEAQAAQLRGTAERMFARRRALEAESRRLNQLLAGQLRPGVAADPDSVRHLTD
                     SLLTLRVTYARLYQDEVAEMGRYLSPVQCAQYYLLRERLLQRIQQARQARTMDGPEGA
                     EPDPGS"
     gene            2576..2660
                     /locus_tag="VFU45_00880"
     tRNA            2576..2660
                     /locus_tag="VFU45_00880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2609..2611,aa:Leu,seq:caa)
     gene            2686..3750
                     /locus_tag="VFU45_00885"
     CDS             2686..3750
                     /locus_tag="VFU45_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357642.1"
                     /translation="MILRRTIPLALAAALASLGACNDLPTSLPIVPTTWILPIKADTI
                     SVAQFLPASVDTAGGLFVTSVKRDSIRQSLGQMCAACGAVNGTTDSVPAFTVTLAHAD
                     SFPSQVISITPGGFALSYRLDNNLGFDPLRPGVGQFGSIVTVLKDSTGAVIATDSVDG
                     ASDSLASGTSLTRNIPLGSNPISGLVNVVATITVPHGDPVTIDTSAAFQVTTLTDTAT
                     LAGVTVQLTSQSIQSDSLSVDWSGIGSDMQSNLQGAELRLDIQNPFTAAGSGSVSFKQ
                     GGTDVIPAKALTFAAGASTDTLSITQSEIQSLTSAGKFSILVNASVSGSGPGQSVALT
                     PTQVATVAGHVLLSIVYGGK"
     gene            3754..5028
                     /locus_tag="VFU45_00890"
     CDS             3754..5028
                     /locus_tag="VFU45_00890"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HEU5357643.1"
                     /translation="MRRLLLAVLVMAAPAALGAQVVPAGARAAGLGDNYGVLARGFGA
                     GYWNPANLGQRGNPAFSLELPGVGVSASADPIKLSDIKKYGGDTLPNSVKQQWLNQIG
                     PNGSLELRTQADVSEAGLSIGPVAVTVGTTAGIRGDLPHDAVATMLYGNAGPSGQGDT
                     LSFTNGALQAFAVSHVGVSLGVPIPVHITHAADERFSVGITAKYVMGHGLLDARDGKG
                     QLTPDSASVRFVTVALYDPNNAISGTGMGFDIGAAWTSGKLAAGLTIYDLANSFRFKD
                     QGKVGSLNAFVSPDSSGGTSNQTPIDSATDSLKQAAQDLASNERFLPSLRLAVGYRLT
                     SRIQLSGDILHESTDPHALVTQKGTTVGVGAELRFIPLIPLRAGVRFGDGATSFSVGT
                     GLALGPVHIDGAVGRRTSTGYNAAVGITFGAW"
     gene            5059..6546
                     /locus_tag="VFU45_00895"
     CDS             5059..6546
                     /locus_tag="VFU45_00895"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU5357644.1"
                     /translation="MTLDITVASAAPGTRATACLVLAVPQGPLPASLAGVDTATGGAL
                     ARLYAAGDFAGKKDEVAVLYPPGPAARIVVVGLGKGEALTRTQLRRGAAIGAKRARSL
                     GAGAAEFYLAPEGRGPVTPRDAGQVVAEGLGQGAWQFTEMKRPPEEPKPPLGSVVILA
                     PADTAEWTAGAATGAAVATGQALTRRIQMLPGNVCTPTFIAEQARELAARHKLKVTIL
                     DRAAIVQEGMGALLAIAQGSAEEPRFIALEFDGAAGKPVVLVGKGVTFDTGGISIKPA
                     QSMEDMKFDMSGAAAVLGAFEALGRIRPKVRVVGLIPSAENMPSGTAVKPGDVVKSHL
                     GKTIEVINTDAEGRLLLADALSYARRYDPAVVLDAATLTGAIVIALGHTALGIMGNDD
                     AVVEEVRRAGERAGERGWPLPLWEEYRELNKSDIADVRNTGGRPAGSITAGWFLREFA
                     ESFPWAHLDIAGTAYSDREDPASAKGPTGMGVRLFTEFVLGRAGK"
     gene            6533..>6734
                     /locus_tag="VFU45_00900"
     CDS             6533..>6734
                     /locus_tag="VFU45_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357645.1"
                     /translation="MRGSSRLGLFLLCTGAALGGPLPLAAQNPDSLAADTVLTDSLRR
                     PVDETQKLLDGQTLELRRVPVPS"
BASE COUNT          957 a         2418 c         2403 g          956 t
ORIGIN      
        1 cctggccggc ggccggcgga gcgagcgtgg cgcacgcgag ggccgtgagc aggagggagc
       61 ggggcgtcac agggtcaaga atggtgcgcc cgcggccggc cgtccagcgg tcacggccgg
      121 ggggacaggc ccaccgcggc ccgggtgcgg tcggggaaat tgccgcagag gccgtcgacg
      181 ccgaggtgcg acagccgcgc gagctcctcc ggggtgttcg cggtccagac gatgacgccc
      241 ttgcctgccg cgtggagcgc agcgaccagg tcggcgtcgg tgagcgaact ctcctgccag
      301 aggacgcccg cgccggccgc cgatacctgc gccaccgggt cgatcgggcg gctggcggag
      361 aggaccccca gccggagtcc aggcctgcgg gcgcccaggc gcgcgatgat ccggtggtcg
      421 aacgcgtgga cgccgtagcg ggacggggcg ggacctcgcg cgatcgtctc gagcaggcgg
      481 tcgtcccagc gcgccgggag ggtcttcacc tcgatccaga cgtccagcgc cccggcagcg
      541 gccaatgccg catcgagggt cgggatcggt tccccgttgg ggagcgtcac gcgctgaacg
      601 tccgcgaacg tatgctcggc gatgatcccg agcccttcga cctcgggatc gtgatggacc
      661 accagtgcgc cgtcggtggt ggcgtggatg tcgagctcga tcccgtcggc gcccatctcc
      721 cgggcgagcg caaacgcagc gatcgaattc tggtacgcat aacccgaggc accgcggtgg
      781 gcgatgacgc tggcacgggg catcaggcac gttcctcgcg gtagatggct gacgtagctt
      841 aacactgtcc tgtccccacc gtctgccgag gaccgatgac cccagcgccg gagtccggag
      901 ccccggagga cgtggacctg gtcgcaggct ggcgcgcggg cgatcaggcg gccgcgaccg
      961 cgctggtcgc gcggcacacc ggtgcggtgg cccgcttcct tcactcctcc ggcgcgcctc
     1021 cggcagacct cgatgacctg gtgcaggagg cattcttccg ggccttccgg cggatcgaca
     1081 gctggcgcgg agatgcgggg ttccgcgcct ggctcatcag catcgccggc aacctcctcc
     1141 gcgaccagtg gcggcggcgc aaggggcgcc ggatcctgcc gctggatgac cgggacctgg
     1201 caggggccga cgcgcccgac ggcgaggtcg aggccgatga ggccgctgag cggatccgtg
     1261 ccgagctcgg tcgattgccc cggctgcagc gggaggtctt cctcctacgg gtccaggagg
     1321 gcgcgggtta cgaggaaatc gcccgcgcac tcggcaccac tagcggggcg gcccgggtgc
     1381 actaccatca cgcagtgcgg cggctgaagg agcgggttcg atgaccagct gtgaggggct
     1441 ccaggagcgg atgccggcgg tcgcgcggga tgaggatcgt tggagcgctg ccgatcttgc
     1501 ccacgtcagg ggctgcgcgg agtgctcggc cgagtggtcg gtggtggagg ggaccgtccg
     1561 gcttggccac gccactcccg cggtggaccc ggggcggacg gccgccgccg tggtgcggcg
     1621 cctcggtgcc gaccgcgtcc gggcgcgggt ccggcgccgg tgggtcgggg gagcggtcgg
     1681 ggtggccgcg gcggcggcgc tcgcgttcgt gctgtggccg gccgcgcggc cagcccccgc
     1741 cgcgccggtg gaactgcagg catcggtctt ccttccggaa ctggattcgc tcaccgtcga
     1801 ccagctcaag gcggtcctcg gtgcggtgga ccgccccctc ggctccgacc ggaccctcga
     1861 ttcctcgggg ctgagcggac tgtcggacga cgaactctcg gccgtactcc aatcgatgga
     1921 ggggtgatgc gcttcctggg tcggtccctc gccgcgatcg cgctcatcgc cgcgccgctc
     1981 accgcgcagc gggtcccccc ggaccagcgc ggcgtgctgc aacggcggat cgagaacatc
     2041 ttcctggccc gcgtcaccga ggagctgggg ctcaccgagg cgcaggccgc ccagctgcgg
     2101 ggcacggcag agcggatgtt cgctcgtcgc cgggcgctcg aggccgagtc gcgaaggctc
     2161 aatcagctcc tggccggtca gctgcgcccg ggtgtcgccg ccgacccgga cagcgttcgc
     2221 cacctcaccg acagcctgct caccctgcgg gtgacgtacg ccaggctgta tcaggatgag
     2281 gtggccgaaa tggggcgcta cctctcgccg gtgcagtgcg cccagtacta cctgctgcgg
     2341 gagcggctcc tgcagaggat ccagcaggcg cgccaggcgc gaacgatgga cggcccggaa
     2401 ggcgcagagc ccgatccggg gtcctgacgt tgaaggcccg ttcgggatgg ggcgacctgc
     2461 aaggatctcc ctgtcggtgt gcgctggatc acctcaaata tccttcgacg acagtacctt
     2521 aggcccgttc ttgcgacttg cggaagccgc gcggatgggc gagcttgttg cccaagcccg
     2581 agtggcggaa tggcagacgc agcggactca aaatccgccg gggggcgacc cccgtgtggg
     2641 ttcgactccc acctcgggta tgatctcccg agcgaggccc caaccatgat cctgcgacgt
     2701 acgatccccc ttgcgctcgc cgcggcgttg gcgagcctcg gcgcctgcaa cgacctcccc
     2761 acgtcactcc cgatcgtccc caccacctgg atcctcccga tcaaggcgga cacgatctcg
     2821 gtggcgcagt tccttccggc ctcggtcgac accgccggag ggctcttcgt gaccagcgtg
     2881 aagcgggact cgatccgcca gtccctggga cagatgtgcg cggcctgcgg cgccgtcaac
     2941 gggaccacgg actccgtccc ggcattcacc gtgacgctgg cccatgcgga ttccttcccg
     3001 tcgcaggtca tctccatcac gccggggggc ttcgcgctct cgtaccggct cgacaacaac
     3061 cttggattcg atccgctgcg ccccggcgtc ggtcagttcg ggtcgatcgt gacggtcctc
     3121 aaggactcga ccggcgccgt gatcgcgacc gacagcgtgg atggtgcgag cgacagcctc
     3181 gcgagcggca cgtcgctcac ccggaacatc cccctcgggt cgaacccgat ctccggcctg
     3241 gtcaacgtcg tggccaccat cacggtgccc cacggcgacc cggtcacgat cgacaccagt
     3301 gccgccttcc aggtcaccac gctgaccgac accgcgaccc tggccggcgt gacggtgcag
     3361 ctgaccagcc agagcatcca gagcgactcg ctgagcgtgg actggtccgg catcggctcc
     3421 gacatgcagt cgaacctgca gggtgccgaa ctccggctcg acatccagaa cccgttcacc
     3481 gccgccggca gcggctccgt gagcttcaag cagggaggca ccgacgtcat ccccgccaag
     3541 gcgctcacgt tcgctgcagg cgcgtccacc gacacgctca gcatcacgca gtccgagatc
     3601 cagtcactga cctctgccgg gaagttctcg atcctggtca acgcctcggt cagcgggtcc
     3661 ggacccgggc agtcggtggc cctcacgccg acccaggtgg ccaccgtggc cgggcacgtg
     3721 ctcctgtcca tcgtctacgg ggggaagtga acgatgcgtc gactcctcct ggcggtcctg
     3781 gtgatggcgg cgcccgcggc cctcggcgcc caggtggtcc cggcgggcgc gcgcgccgcc
     3841 ggcctcggtg acaactacgg cgtgctggcg cgcgggttcg gcgccggcta ctggaatccg
     3901 gccaacctcg gccagcgggg gaacccggcc ttctccctcg agctgccggg cgtcggcgtc
     3961 tcggcctcag ccgacccgat caagctgtcg gatatcaaga aatacggcgg cgacacgttg
     4021 ccgaactcgg tgaagcagca gtggctcaac cagatcggcc cgaacggctc gctcgagctc
     4081 cgcacccagg cggatgtgag cgaggccggc ctgtccatcg gtcccgtggc cgtcaccgtc
     4141 gggacgaccg ccggcatccg cggcgacctg ccgcacgatg ccgtcgccac gatgctctac
     4201 ggcaacgccg gccccagcgg ccagggcgat acgctctcgt tcaccaacgg tgcgctccag
     4261 gccttcgccg tctcccatgt cggcgtgagc ctcggcgtcc cgatcccggt ccacatcacc
     4321 cacgccgccg atgagcggtt ctccgtcggc atcaccgcca agtacgtgat ggggcatggc
     4381 ctcctcgacg cccgcgacgg caagggacag ctgaccccgg acagcgcgtc ggtccgcttc
     4441 gtcaccgtgg ccctgtacga tccgaacaac gccatcagcg gcaccgggat ggggttcgac
     4501 atcggcgcgg cgtggaccag cggcaagctc gccgcggggc tcacgatcta tgacctggcc
     4561 aactcgttcc gcttcaagga ccaggggaag gtcgggagcc tgaacgcctt cgtctcgccg
     4621 gacagcagcg gcgggacgtc gaaccagacc cccatcgact cggccaccga ctcgctcaag
     4681 caggcggccc aggacctcgc cagcaacgag cgcttcctgc cgagcctgcg cctggccgtc
     4741 ggctaccggc tcaccagccg gatccagctg tcgggcgaca tcctgcacga atccaccgac
     4801 ccgcacgcgc tggtgaccca gaagggcacg accgtcgggg tcggtgcgga gctgcggttc
     4861 atcccgctca tcccgctccg cgccggcgtc cggttcggcg acggggcgac gtcgttctcg
     4921 gtcggcaccg ggctcgcgct cgggccagtg cacatcgacg gcgcggtggg gcggcggacc
     4981 tcgacggggt acaatgccgc cgtcgggatc accttcggcg cctggtgact ttcacccgct
     5041 aggcattacc cttccggtat gaccctcgac atcaccgtcg cgtccgccgc gccggggacg
     5101 cgtgccaccg cgtgcctggt gctcgccgtg ccccaggggc cgctccccgc gtccctcgcc
     5161 ggcgtcgaca cggccacggg cggcgccttg gcgcgcctct acgccgccgg cgacttcgcg
     5221 ggcaagaagg atgaggtcgc ggtgctgtac ccgccggggc cggcggcccg catcgtggtg
     5281 gtcggcctcg gcaagggcga ggcgctcacc cgcacgcagc tccgccgcgg tgctgcgatc
     5341 ggggccaagc gcgcccggag cctcggggcc ggtgcggccg agttctacct ggcgccggag
     5401 gggcgggggc cggtgacgcc gcgcgacgcc ggccaggtgg tcgccgaagg cctcgggcag
     5461 ggcgcgtggc agttcaccga gatgaagcgc ccgccggagg agccgaagcc gccgctcggg
     5521 agcgtcgtga tcctcgcgcc ggccgacacc gcggagtgga ccgcgggcgc cgcgaccggg
     5581 gccgcggtgg ccacggggca ggcgctcacc cggcgcatcc agatgcttcc gggcaacgtc
     5641 tgcacgccga cgttcatcgc cgagcaggcg cgcgagctcg cggcccgcca caagctcaag
     5701 gtgacgatcc tcgaccgggc ggccatcgtg caggagggga tgggcgcgct gctcgccatc
     5761 gcgcagggga gcgccgagga gccgcgtttc atcgcgctcg agtttgacgg ggccgcgggg
     5821 aagcccgtgg tgctggtggg gaagggcgtc accttcgaca ccggcggcat ctcgatcaag
     5881 ccggcacaga gcatggagga catgaagttc gacatgtccg gcgccgcggc ggtgctcggc
     5941 gcgttcgagg cgctgggccg catccgcccg aaggtccgcg tggtggggct catcccctcg
     6001 gcggagaaca tgccgtcggg caccgccgtc aagccgggtg acgtcgtgaa gagccacctc
     6061 ggcaagacca tcgaggtgat caacaccgac gccgaggggc ggttgctcct tgccgatgca
     6121 ctgtcgtacg cccgccggta cgatccggcc gtggtgctcg acgccgccac cctcacgggc
     6181 gccatcgtga tcgcgctggg gcacaccgcg ctcggcatca tgggcaacga cgacgccgtc
     6241 gtggaggagg tccggcgggc gggggaacgt gcaggcgagc gcgggtggcc gctgccgctc
     6301 tgggaggagt accgcgagct caacaagtcg gacatcgccg acgtgcgcaa caccggcggc
     6361 cggccggcgg gcagcatcac cgcggggtgg ttcctccggg agttcgccga gagcttcccc
     6421 tgggcccacc tcgacatcgc cggcacggcg tacagcgatc gcgaggaccc cgccagcgcc
     6481 aagggcccca ccgggatggg tgtccgcctc ttcactgagt tcgtgctggg ccgtgcgggg
     6541 aagtagccgc ctcggcctgt tcctcctgtg caccggggcc gcgctgggcg gccctttgcc
     6601 gttggccgcg cagaatcccg actccctcgc cgccgacacc gtgctcaccg actcgctccg
     6661 ccgaccggtc gacgagacgc agaagctcct cgacggccag acgctcgagc tccgccgggt
     6721 gcccgttccc tcgc
//
LOCUS       DASUAP010000035         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_123553, whole genome shotgun sequence.
ACCESSION   DASUAP010000035 DASUAP010000000
VERSION     DASUAP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123553"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFU45_00905"
     CDS             complement(<1..560)
                     /locus_tag="VFU45_00905"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU5357646.1"
                     /translation="MPHLYTLFDTPFGRSGIAWDERGITRIQLAEASAAATAARLEAH
                     GARRARITPPPHVQDAAQRVRRHLAGKPQDFSSVPLALDGLPAPRRRIYQAARAVPAG
                     ATVSYGELAASIGSPGAARAVGAAMAENPFILAVPCHRVMGSNGQLHGFSAHGGIETK
                     RRILALEGVRVYIPRHAPRRYPDGLE"
     gene            730..>2050
                     /locus_tag="VFU45_00910"
     CDS             730..>2050
                     /locus_tag="VFU45_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU5357647.1"
                     /translation="MDLDAFRTNAHRIADWMADYLASLPDRPVTPSVEPGHVRRQIPA
                     MPPAAGEPFDRILADFERIVLPGMSHWQHPGWFGYFPADTSPASVLAEMLTAAMGANC
                     MSWATCPAGTETEQAMMEWLRSLIGLPTTFTGVIQDTASSATLVAILSARERATGFGW
                     ARQGASAEDARRLTVYASREAPRSIAKGVRLAGIGEERLRSIGTDSTYAMRPDLLAAA
                     MEADRAAGLKPACVVATVGTTSSTAIDPVPAIADIAARYGAWLHVDAAYAGSAAVLPE
                     QRHLLAGVERADSFVFNPHKWLFTNFDCSAYFVRDVDTLVRTCGIHPEYLRSVHDEQV
                     ANYRDWGIPLGRRFRALKLWFVIRDFGVEGLQARIRQHIALAQEFRGWVEAASDFEPM
                     APSPLGLVCFRHRPTGMHPDDSAVDDLNRRLLAAINATGRALLTHTTL"
BASE COUNT          292 a          742 c          733 g          283 t
ORIGIN      
        1 tactcaaggc catcgggata ccggcgcggg gcgtggcgcg ggatgtagac ccgcacccct
       61 tcgagcgcca ggatgcggcg cttggtctcg atcccgccgt gggcggagaa gccgtgcagc
      121 tggccgttgc tccccatcac ccggtggcac gggaccgcca ggatgaaggg attctcggcc
      181 atcgccgcgc cgaccgcgcg cgcggcgccg ggcgagccga tgctcgcggc gagctcgcca
      241 taggagaccg tggcgccggc cggcaccgcc cgcgcggcct ggtagatccg tcgccgcggg
      301 gcggggaggc cgtcgagggc gagggggacc gacgagaagt cctggggctt gcccgcaaga
      361 tgacggcgga cacgctgggc ggcatcctgg acatgcggcg gcggagtgat gcgtgcacgg
      421 cgcgcgccgt gggcctcgag ccgcgcggca gtggccgcag ccgacgcctc ggcgagctgg
      481 atgcgggtga tcccgcgttc gtcccacgcg atgccggacc ggccgaacgg ggtgtcgaag
      541 agggtataga ggtgaggcac gggttagaat ataacggacg gggctacccg gttcttgcga
      601 atgggtcgtg ggtcgagggt cacgggtcgt gagtccaggc acgacgacgc ccgatccacc
      661 gtgcgctccg cacccaccgt gtcatatcgt tcgcccacga cccacgaccc acgatccgcg
      721 accatctcca tggacctcga cgccttccgc accaacgccc accgcatcgc cgactggatg
      781 gccgattacc tcgcgtcgct gccggaccgc ccggtgacgc cgtcggtcga gcccggccac
      841 gtccgtcggc agatcccggc gatgccgccg gcggccgggg agccattcga ccggatcctg
      901 gccgacttcg agcggatcgt gctccccggg atgagccact ggcagcaccc ggggtggttc
      961 gggtacttcc cggccgacac gagcccggcg tcggtcctgg ccgagatgct gaccgcggcg
     1021 atgggcgcca actgcatgag ctgggcgacc tgccccgccg ggaccgagac cgagcaggcg
     1081 atgatggaat ggctccgcag cctgatcggg ctccccacga cgttcaccgg ggtcatccag
     1141 gacaccgcct cgagcgccac gctggtggcg atcctctccg cccgggagcg cgccaccggc
     1201 ttcggctggg cccggcaggg tgcctccgcg gaagacgccc ggcggctcac cgtctacgcc
     1261 tcgcgcgagg cgccccgctc gatcgccaag ggtgttcggc tcgcggggat cggcgaggaa
     1321 cggctgcgat cgatcggaac ggacagcacg tacgccatgc ggccggacct gcttgcggcg
     1381 gcgatggagg ccgaccgcgc ggcggggctc aagccggcgt gcgtggtggc cacggtgggg
     1441 acgacttcgt ccaccgcgat cgacccggtg ccggccatcg ccgacatcgc ggcgcggtac
     1501 ggtgcctggc tccacgtcga cgcggcctac gccggttcgg cggcggtgct cccggaacag
     1561 cggcacctcc tcgccggcgt cgagcgggcc gacagcttcg tcttcaaccc ccacaagtgg
     1621 ctcttcacca acttcgactg ctcggcctac ttcgtccgcg acgtcgacac gctggtgcgg
     1681 acctgcggca tccatcccga gtacctccgg tccgtccacg acgagcaggt ggccaactac
     1741 cgcgactggg gcatcccgct gggccggcgc ttccgcgcgc tcaagctctg gttcgtgatc
     1801 cgcgacttcg gggtggaggg gctgcaggcc cggatccggc agcacatcgc actggcgcag
     1861 gagttccgcg gatgggtcga ggcggcctcc gacttcgagc cgatggcgcc atcgccgctc
     1921 ggactggtgt gcttccggca ccgtcccacg gggatgcacc ccgacgactc cgcggtcgac
     1981 gacctcaacc gccggctcct cgccgcgatc aacgccaccg gcagggcgct cctcacgcac
     2041 accacgctcg
//
LOCUS       DASUAP010000036         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_491449, whole genome shotgun sequence.
ACCESSION   DASUAP010000036 DASUAP010000000
VERSION     DASUAP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491449"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VFU45_00915"
     CDS             <1..1132
                     /locus_tag="VFU45_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5357648.1"
                     /translation="PPTQLPLLDSPAPWRELARHERGARYVRIEPRRVLNGPESTGMG
                     FWSINPYIGCEFGCTYCYARDTHRWAVERAGGRMDAWADGRMAEETVEVDKPAEAIIR
                     PSDHPSTRLPAWLAFEKVILVKEEAAAVLAGSFAPAQVMGDTIIIGTATDPYQPAEKM
                     FGVTRRILERFLGYRGLKLGIITKSPLVVRDIDLLVRLSALHELKVNISLASLDRQMV
                     RRLEARSPAPQARLRALKALTDAGVEAGLMVAPIVPGLTDGRAALRALLVAGKEAGAR
                     YAYGSALRLGPAARNRFLPHLARKFPALQERYARRYGRSDKAGKDYEDALSARFRALQ
                     EELGYTTQISFTRNKRGTTEGHGESRRNAVVVGDQIGSVDQD"
     gene            1240..1743
                     /locus_tag="VFU45_00920"
     CDS             1240..1743
                     /locus_tag="VFU45_00920"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5357649.1"
                     /translation="MPTITELLLPEFDREMASTRKVLERVPEAKGAWKPHPKSYTLGD
                     LAVHVATLPHWGTVTFRDSGFDYTEKVAAEMARKFEGTKALLAMFDRHSAEARAELLK
                     VADPAWSAPWTFKYQGQPVFTKPRYETYRTDTLNHLVHHRAQLTVYLRLNDVPLPNLY
                     GPTADEK"
BASE COUNT          342 a          726 c          671 g          318 t
ORIGIN      
        1 gccacccacc caactccccc tcctcgactc ccctgcccct tggcgggagc tcgcgcgcca
       61 cgagcgcggc gctcgctacg tgcggatcga gcccaggcgg gtgctcaacg ggccggagtc
      121 gacggggatg ggcttctggt caatcaaccc ctacatcggc tgcgagttcg ggtgcaccta
      181 ctgctacgcg cgggatacgc accggtgggc ggtcgagcgg gcgggtggcc gcatggacgc
      241 ttgggcggac ggacgcatgg cggaggagac ggtcgaagtt gacaagccgg ccgaggcgat
      301 catccgacca tccgaccatc cgtccacgcg ccttcccgcc tggctcgcat tcgaaaaggt
      361 catcctcgtc aaggaagagg ccgccgcggt gctcgctgga agctttgcgc cggcccaggt
      421 catgggcgac acgatcatca tcggcacggc caccgacccg taccagcccg ccgagaagat
      481 gttcggcgtc acccggcgga tcctcgagcg gttcctggga taccgcgggc tcaagctcgg
      541 catcatcacc aagtcgccgc tggtggtgcg cgacatcgac ctgctggtgc ggctttcggc
      601 actgcacgaa ctgaaggtga acatttccct cgcctcgctc gaccgccaga tggtccgtcg
      661 gctggaggcc cgctcacccg cgccgcaggc ccgcctccgg gcactcaagg cgctcaccga
      721 tgcgggcgtc gaggccgggc tcatggtggc gccgatcgtc ccgggcctca ccgacgggcg
      781 ggcggcgctc cgggcgctcc tcgtggccgg gaaggaagcg ggggcgcgct acgcgtacgg
      841 gtcggcgctc cgactcggac ccgcggcgcg caaccgtttc ctcccccacc tggcccggaa
      901 gttcccggca ctccaggaac gctatgcgcg gcggtatggc cggtcggata aggcggggaa
      961 ggattacgag gacgcgctgt cagcgcgctt ccgggcgctc caggaggagc tcgggtacac
     1021 tacacagatc tcgttcacgc ggaacaagcg tgggaccacg gaggggcacg gagaatcacg
     1081 gagaaatgca gttgttgtgg gtgaccagat cggctccgtg gaccaggact gaggccggcc
     1141 ggtcccgcca catcgaccgg cagttctccg tctctctccg tgttactccg tgccctccgt
     1201 ggtcccacac gtcaccatca ctcagaagag gacgatagaa tgccgaccat caccgaactg
     1261 ctgctccccg aattcgaccg cgagatggcg tccacccgga aggtcctcga gcgcgtcccc
     1321 gaggcgaagg gcgcctggaa gccccacccc aagtcctaca ccttgggcga cctcgcggtc
     1381 cacgtcgcca ccctgcccca ctgggggacg gtgacgttcc gcgacagcgg cttcgactac
     1441 accgagaagg tcgcggccga gatggcacgg aagttcgagg ggacgaaggc gctcctcgcg
     1501 atgttcgacc gacactcggc ggaggcgcgg gcggaactgc tcaaggtggc cgacccggcc
     1561 tggtcggccc cgtggacctt caagtaccag gggcagccgg tcttcaccaa gccgcgctat
     1621 gagacgtacc gcaccgacac gctcaaccac ctggtgcacc accgggccca gctcacggtg
     1681 tacctgcggc tcaacgacgt cccgctcccg aacctctacg ggccgacggc ggacgagaag
     1741 taggcgcatg ggcgcatgag gcgcgtggac gcatgaaaga ccgcatggaa accgagtaaa
     1801 gactgcgtgc tgtttacgcg ccgatgcggc ctttcatgtg gctgttgacc cgcctatgcg
     1861 gctgttcatg cggcagttca tgcgcctgtt cgtgcggcct ctcacgcgcc acccacctac
     1921 aacatgatct tcgccagctc cggccccttc atctcggggc cgccgttgtc catcggctcc
     1981 acgctcagcg cggcgcccat caccttggcg tctcccggcg gcatgcccgt ggtgaacgtg
     2041 gcgaccttcc ccggcac
//
LOCUS       DASUAP010000037         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1349974, whole genome shotgun sequence.
ACCESSION   DASUAP010000037 DASUAP010000000
VERSION     DASUAP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349974"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFU45_00925"
     CDS             <1..435
                     /locus_tag="VFU45_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU5357650.1"
                     /translation="VGLGGDLSAAGEAPVGGWPVRVTDNHAHPLDGPGPVIALASGGL
                     ATSTTTVRRWTRGGDTVHHLLDPATGLPPPEHWRTASVAAGSCVDANIASCASILLGA
                     GAPSWLNDLGLPARLVNSSGGVTLVGGWPESDPIDNEAVLTP"
     gene            432..1544
                     /locus_tag="VFU45_00930"
     CDS             432..1544
                     /locus_tag="VFU45_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU5357651.1"
                     /translation="MTSAIGTNSQALWYMTRGFGLIDLVLLTVTMVMGLTQVVRYARP
                     GWPRFVVSALHKNVSLLAVVFLAVHVVTAVLDTFAPISIASVFIPFVGKYRPLWLGLG
                     ALSLDLLIALVITSLMRERMGQRAWRLLHWAAYLCWPVAIVHGLGTGSDTKLGWVLAI
                     NAFCVLAGVAAIWWRLAERWRTAPERTPAAASKRLAAVGGTLAVPLLVGVWLLQGPLH
                     PGWARRAGTPANLVGASPAASSSASGQGSSGASQGSTASLAIPFQSNFRAVERRGGTD
                     SSGQVTIVFDGTFSSGSFRVTLLGSPADEGVQLSSGRVTLGPAGSPSQYQGNVTQLRG
                     DTIVANLSGAGRSFTVTIRLTGRDGGNLSGVIEASS"
     gene            1541..>2718
                     /locus_tag="VFU45_00935"
     CDS             1541..>2718
                     /locus_tag="VFU45_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU5357652.1"
                     /translation="MTHPRTESRRDAHHAPPATSLPRLLATADDPGFDAHFRQWGQMP
                     AGGPLLIDEVDRAGLRGRGGASFPTARKLAAVSQGGATPVVVANGTEAEPLSLKDKTL
                     LSSAPHLILDGVSIAAETVGADQAIVCVERSSPGTRVLRQALSERENVRADTVRINLE
                     EVPSRYVAGEESALVHWLNGGDAKPTFVPPRPFQRGVAGRPTLVNNVETLANLALIAR
                     YGADWFRSVGTAEDPGSALITILGDVAHPGVYEFALGTPVQTALRSAGVGPSAHAVLS
                     GAYAGTWLPAGRAANLTVDRASFSAANAALGCGSLFVHGSQYCGLATTARIAKWMASQ
                     SAGQCGPCAFGLPAVADALAALAAGDPRGRWEGQLRRWLDVIPGRGACHHPDGTVRMI
                     R"
BASE COUNT          344 a         1013 c          930 g          431 t
ORIGIN      
        1 gtcgggctgg gcggagatct ctccgcggcc ggcgaggctc cggtgggcgg ctggccggtc
       61 cgcgtcacgg acaaccacgc ccatccgctc gacggtccgg gcccggtgat cgcgttggca
      121 tcgggcggtc tggccacgtc caccacgacg gtgcgccgtt ggacccgggg tggcgacacc
      181 gtccaccacc tcctggaccc ggccaccggt ctccctccac cggagcactg gcgtacagcc
      241 tcggtcgccg ccggttcatg cgtcgacgcg aacatcgcct cctgcgcgtc gatactgctc
      301 ggcgccgggg ccccctcctg gctgaacgac ctggggctgc cggcccgcct ggtcaactct
      361 tcgggcggcg tgaccctcgt cggcggatgg cccgagagcg acccgatcga caatgaagcg
      421 gtgctgaccc catgaccagc gcgatcggca ccaactcgca ggcgttgtgg tacatgaccc
      481 gcggtttcgg gctcatcgac ctggtcctgc tgaccgtgac gatggtgatg ggcctcaccc
      541 aggtggtgcg ctacgcgcgc cccgggtggc cccgcttcgt cgtgtcggcg ctgcacaaga
      601 acgtctcgct gctggccgtc gtgttcctgg cggtccacgt ggtgacagcc gtgctggaca
      661 cgttcgcgcc catctccatc gcgagcgtct tcatcccctt cgtcggcaag tacaggccgc
      721 tctggctcgg tctcggtgcg ctctcgctcg acctccttat cgctttggtg atcaccagcc
      781 ttatgcgcga acggatgggc cagcgggcgt ggcggctgct ccactgggcg gcctacctgt
      841 gctggccggt cgcgatagtg cacggcctcg gcaccggctc ggacaccaag ctcggctggg
      901 tcctcgcgat caacgcgttc tgtgtcctcg ccggcgtcgc agccatctgg tggaggctcg
      961 cggaacgctg gcggacagct cctgagcgca cgcccgcagc tgcctctaag cggctcgccg
     1021 ccgtgggggg cacgctggcc gtgccgctgc tcgtcggcgt gtggctcctg cagggcccgc
     1081 ttcaccccgg ctgggcgcgc cgggccggca cgcccgccaa cctcgtaggc gcctcgccgg
     1141 cagcctcttc ttccgcttcg ggccagggct ccagcggcgc gtcgcaggga tccaccgcgt
     1201 cgctggccat ccccttccaa tcgaacttcc gcgcggtgga gcgccgcggc ggcacggact
     1261 cctcggggca ggtgacgatc gtgttcgacg ggaccttctc gtcaggttcg ttccgtgtga
     1321 cactcctcgg ctccccggct gacgagggcg ttcagctcag ctcgggacgc gtgacgctgg
     1381 gtcccgctgg gtcgccgagc cagtaccagg ggaatgtcac ccagctgcgc ggggacacca
     1441 tcgtcgccaa cctctccggg gctgggcgct cgttcacggt cacgatccgg ctgaccggcc
     1501 gcgacggcgg caacctcagt ggtgtgatcg aggcgagctc gtgacccacc ctcggaccga
     1561 atcccgaagg gacgctcacc acgcgcctcc cgcgacatcc ctgccgcgcc tgctggccac
     1621 cgctgacgat cccggcttcg acgcccactt ccggcagtgg ggccagatgc ccgccggcgg
     1681 gcctcttctc atcgacgagg tcgacagagc ggggctgcgc ggaagaggag gagcgtcgtt
     1741 ccccaccgcc cggaagctgg ccgcggtatc gcagggcggc gcgaccccgg tcgtcgtcgc
     1801 caacgggaca gaggcggagc ccctcagcct caaggacaag accctcctct cctccgcccc
     1861 gcacctcatc ctcgacgggg tcagcatcgc ggctgagacc gtgggcgccg atcaggcgat
     1921 cgtctgtgtc gagaggagct ctcccggcac tcgggtgctc cgccaggcgc tctccgagcg
     1981 cgagaacgtg cgggccgaca ccgtccggat caacctcgag gaggtcccgt cgcgatacgt
     2041 cgcaggcgag gagtcggcgc tcgtgcactg gctcaacggg ggtgacgcca agccgacctt
     2101 cgtcccgccg aggcctttcc agcgcggcgt cgccggccgg ccgaccctcg tcaacaacgt
     2161 ggagaccctc gccaacctgg cgctcatcgc ccgctacggc gcggactggt tccgctcggt
     2221 cgggaccgcg gaggacccgg ggagcgcgct cataaccatc ctcggtgacg tggcccatcc
     2281 gggtgtctac gagttcgccc tcggcacccc ggtgcagacc gcgctgcgca gcgccggcgt
     2341 aggtccgtcc gcgcacgcgg tcctctccgg ggcgtacgcg ggaacctggt tgcctgccgg
     2401 aagggcagcc aacctgacgg tcgaccgggc ttcgttctcg gccgccaacg ccgcgctggg
     2461 atgcggttcg ttgttcgtgc acggcagcca gtactgcggg ctcgccacca cggcccgcat
     2521 cgcgaaatgg atggcgtccc agagcgccgg ccagtgcggg ccgtgcgcct tcggcctgcc
     2581 cgcggtcgcg gacgccctgg ccgccctcgc cgccggtgac ccccggggtc gctgggaggg
     2641 ccagctgcgc aggtggctcg acgtgattcc cgggcgcggt gcctgccacc accccgacgg
     2701 gaccgtccgg atgatccg
//
LOCUS       DASUAP010000038         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_55419, whole genome shotgun sequence.
ACCESSION   DASUAP010000038 DASUAP010000000
VERSION     DASUAP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55419"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..2319
                     /locus_tag="VFU45_00940"
     CDS             163..2319
                     /locus_tag="VFU45_00940"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU5357653.1"
                     /translation="MTVAIADLAPGFRAALERHADRLDLDVIDRALRFSASAHRGQKR
                     MSGDDFVSHSIAVAQILVDQLLDTTSIAAALLHDVVEDSAVRLDDIVREFGPEIAGLV
                     DGLTKISHLTFRSTTEAQVENYRKLLLSVAKDARVIIIKLADRLHNMRTLEHLTPEKR
                     ERIATETREIYAPLAHRFGMAGVKAELEDLAFKFLEPADYQGLAEQVAAKRTAREASI
                     LKLRTPLEFELTKAGIGWFEVTGRPKHLWSIYRKMRSRDKPFDEIYDLMAVRVMVKTV
                     ADCYHVLGIIHHVWTPIQERIKDYIASPKSNGYQSLHTTIFGPGGQLFEVQIRTQEMH
                     RTAEYGIAAHWLYKDGETPDELDRQLGWFRQLLELQQDAASPEEFLEFLKIDLYPDEI
                     FVFTPQGDVKRLPKGATAIDFAFHVHTEVGLRCQGARVNGRIAPLHRALKNGDTIEIL
                     TGPSARPSRDWLNHVHTARARHKIKQWINHEEERVQETMGRELLGREVKRRRLDAPDD
                     ARMERAAAALSVNGAAGLAVAVGRGDVAIGAVIKALFPDLPDDELVEPRPTVFGRVID
                     KFRQRKGIRIQGADGLLVRYAQCCQPLPGDAVVGYVTQGRGISIHRADCPNLLTLPVD
                     GRRVDIDWQEVEGESFAVRLAVSGDDRRGLYADLMSAVSSTGTNIKGAEIQSKDGTMY
                     GTVLVEVDSLPHLAKVLKAMRRVKGVSEVERREAGN"
     gene            2385..3230
                     /locus_tag="VFU45_00945"
     CDS             2385..3230
                     /locus_tag="VFU45_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5357654.1"
                     /translation="MAQRSPTAQVVTLGTGNPFPDPDRQGPATAVTVGPRVFLFDAGA
                     GVERQLRAAALPISGPTALFLTHLHTDHTLGLADLILTSWVMRRTRPFPIYGPRGTKA
                     MVRHLLAAYGEDIRIRTDGLEHEVKDGWRVVVHEIGPGVAFDSGGVRVTAIPVRHGNW
                     PVAFGYRIDAPGRSIVISGDTRPSDSLAVAATGVDVLVHEAYPASRVAPEDRPGGKEW
                     PAYLRAFHTSDEELGALAARARPKLLVVTHVEWMGASDSEVVSGIRRGGFTGNVAIAK
                     DLDRF"
     gene            3309..3692
                     /locus_tag="VFU45_00950"
     CDS             3309..3692
                     /locus_tag="VFU45_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU5357655.1"
                     /translation="MAYWLLKTEPSTYAIGDLQREKRTRWDGVKNAAALINLRKMAVG
                     DQVLIYHTGAEKAIVGVARVFVADPKDGVITLEFERVLRRPVPLAAIKADPAFKDLAL
                     VRISRLSAMPVSPAHWTRLLKPSGP"
     gene            complement(3673..>3869)
                     /locus_tag="VFU45_00955"
     CDS             complement(3673..>3869)
                     /locus_tag="VFU45_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357656.1"
                     /translation="GEEEEKWRRRRRTRSGGRRRGREEEEKWRRRRRRRRRSGGRRRG
                     REEEEKRRRRARDGIRVRSA"
BASE COUNT          574 a         1449 c         1248 g          598 t
ORIGIN      
        1 gtcaggtcga caccaacgcg gcgcgcgcac tgggacacgg ggggaactcc tgcccccagc
       61 cggagccgcg ccccttcccg gactgaccga cggccgcacg cccctccccc tgcggccggg
      121 caccccgcgc cggccgcatt agtttaggga ggggagggtg ccgtgaccgt agccatcgcc
      181 gacctcgcgc ccggcttccg cgccgcactc gagcgccacg ccgaccggct ggacctggat
      241 gtcattgacc gggccctccg cttcagcgcg tcggcgcacc ggggccagaa gcggatgtcg
      301 ggcgacgact tcgtgtcgca ctcgatcgcg gtggcccaga tcctggtgga ccagctcctc
      361 gacaccacgt cgatcgcggc ggcgctcctg cacgacgtgg tcgaggattc cgcggtccgg
      421 ctcgacgaca tcgtccgcga gttcggcccc gagatcgccg gactggtgga cgggctcacc
      481 aagatctccc acctcacctt ccgctccacc accgaagcgc aggtcgagaa ctaccggaag
      541 ctcctgctct cggtggccaa ggatgcgcgc gtcatcatca tcaagctggc cgaccggctg
      601 cacaacatgc gcaccctcga gcacctcacg cccgagaagc gcgaacgcat cgccaccgag
      661 acccgcgaga tctacgcgcc gctggcgcac cgcttcggca tggcgggcgt caaggcggag
      721 ctggaggacc tggccttcaa gttcctcgaa ccggccgact accaggggct cgccgagcag
      781 gtcgccgcca agcgcacggc ccgggaggcc tcgatcctca agctccggac gccgctcgag
      841 ttcgagctga ccaaggcggg gatcggctgg ttcgaggtga ccggccggcc caagcacctg
      901 tggtcgatct accggaagat gcgcagccgg gacaagcctt tcgacgagat ctacgacctc
      961 atggcggtcc gggtgatggt caagacggtg gcggactgct accacgtgct cggcatcatc
     1021 caccacgtct ggaccccgat ccaggagcgg atcaaggact acatcgcgag ccccaagtcg
     1081 aacggctacc agtcgctcca caccacgatc ttcggccccg gcggccagct cttcgaggtg
     1141 cagatccgca cccaggagat gcaccgcacc gccgagtacg gcatcgcggc ccactggctc
     1201 tacaaggacg gcgagacgcc cgacgaactc gaccgccagc tcggctggtt ccgccagctg
     1261 ctggagctgc agcaggacgc cgccagcccc gaggaattcc tcgagttcct gaagatcgac
     1321 ctctacccgg acgagatctt cgtcttcacc ccgcagggcg acgtgaagcg gctgcccaag
     1381 ggcgccaccg cgatcgactt cgccttccac gtccacaccg aggtggggct ccgctgccag
     1441 ggggcgcgcg tcaacggccg gatcgcgccg ctgcaccggg cgctcaagaa cggcgacacg
     1501 atcgagatcc tcaccggccc gagcgcccgc ccctcccgcg actggctcaa ccacgtccac
     1561 accgcccggg cacgccacaa gatcaagcag tggatcaacc acgaagagga gcgggtccag
     1621 gagacgatgg gccgcgagct cctcgggcgc gaggtgaagc gccgtcgcct cgacgcgccg
     1681 gacgacgcac ggatggaacg ggccgcggcc gcgctctcgg tcaacggcgc ggccgggctc
     1741 gcggtcgccg tcggccgggg cgacgtggcc atcggcgccg tcatcaaggc gctctttccc
     1801 gacctgcccg acgacgagct ggtggagccc cggccgacgg tcttcggccg tgtcatcgac
     1861 aagttccggc agcggaaggg gatccggatc cagggcgccg acgggctcct ggtccgctac
     1921 gcccagtgct gccagccgct ccccggcgat gccgtggtcg gctacgtgac gcagggccgc
     1981 gggatctcga tccaccgcgc cgactgcccc aacctcctca cgctcccggt cgacgggcgg
     2041 cgggtggaca tcgactggca ggaggtggag ggggagagct tcgccgtgcg cctcgccgtc
     2101 agcggcgacg accgccgcgg cctctacgcc gacctgatga gcgccgtgag ctccaccggc
     2161 accaacatca agggcgccga gatccagtcg aaggacggca cgatgtacgg caccgtgctg
     2221 gtggaggtcg attcgctgcc gcacctggcc aaggtcctca aggcgatgcg gcgggtgaag
     2281 ggcgtgtcgg aggtcgagcg gcgcgaggca gggaactgac cgggatcggc ggccggtccg
     2341 ccctggggct ggcagccgcg ctcctcggcg cggtcccggc gcttctggcg cagcggtccc
     2401 cgacggccca ggtcgtcacc ctcggcaccg ggaacccctt tcccgatccc gatcgccagg
     2461 ggccggcgac cgcggtgacg gtcggcccgc gggtcttcct cttcgacgcg ggcgccgggg
     2521 tcgaacgcca gctccgcgcc gccgcgctcc cgatctccgg tcccacggcg ctgttcctca
     2581 cccatctcca caccgaccac accctcgggc tcgccgacct gatcctgacc tcgtgggtca
     2641 tgcgccggac gcggcccttc ccgatctacg gaccccgcgg cacgaaggcg atggtgcggc
     2701 acctgctggc ggcatacggg gaggacatcc ggatccgcac cgacggactg gaacacgagg
     2761 tgaaggatgg ctggcgggtc gtggtgcacg agatcggccc cggcgtggcg ttcgacagcg
     2821 gcggcgtccg ggtcaccgcc atcccggtgc ggcacggcaa ctggcccgtc gcgttcggct
     2881 atcggatcga tgccccgggg cggagcatcg tgatctccgg cgacacccgg ccgagcgact
     2941 cgctcgccgt cgcggcgacc ggggtggatg tgctggtgca tgaggcgtac ccggcgtcgc
     3001 gggtggcgcc ggaggaccgc cccggcggga aggagtggcc agcgtacctc cgggcgttcc
     3061 acacgtcgga cgaggaactc ggtgccctcg ccgcgcgggc ccgcccgaaa ctgctggtgg
     3121 tgacccacgt cgagtggatg ggagcgagcg attcggaggt ggtgtccggc atccgtcgcg
     3181 gcggcttcac cggcaacgtc gccatcgcga aggacctcga tcggttctga ccgggccggc
     3241 gccacaccca gtcccttttc cctttttcct tttcactctt cacttttccc ttttccctct
     3301 tccctcccat ggcctactgg ctcctcaaga ccgaaccgtc gacctacgcc atcggcgacc
     3361 tccagcggga gaagcgcacg cggtgggacg gggtcaagaa tgccgcggcg ctcatcaatc
     3421 tccggaagat ggcggtgggc gaccaggtgc tcatctacca caccggggcg gagaaggcga
     3481 tcgtcggggt cgcgcgggtc ttcgtggccg atcccaagga cggcgtcatc acgctcgagt
     3541 tcgagcgggt cctccggcgc ccggtgcccc tcgccgccat caaggccgac cccgcgttca
     3601 aggacctggc cctggtgcgg atctcccgcc tgtcggcgat gccggtctcg ccggcgcact
     3661 ggacccgtct cctcaagccg agcggaccct gatcccgtcc cgcgctcttc tcctcctctt
     3721 ctcctcctct tctcttcctc ttctcctccc tccgctcctc cttctccttc tccttctcct
     3781 tctccatttc tcctcctctt ctcttcctct tctcctccct ccgctcctcg ttctccttct
     3841 ccttctccat ttctcctcct cttctcctc
//
LOCUS       DASUAP010000039         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_682370, whole genome shotgun sequence.
ACCESSION   DASUAP010000039 DASUAP010000000
VERSION     DASUAP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682370"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFU45_00960"
     CDS             complement(<1..210)
                     /locus_tag="VFU45_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEU5357657.1"
                     /translation="MWTGRRVVLGVSGGIACYKSCVLARRLTEAGVQVDVVMTRAAAE
                     FVRPLTFEALTGRPVLSSLWEPGGAL"
     gene            complement(216..428)
                     /locus_tag="VFU45_00965"
     CDS             complement(216..428)
                     /locus_tag="VFU45_00965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU5357658.1"
                     /translation="MRIVTPKEVAQQSGNKYLGVLVAAKFARYLNEFPKGSEAIGDEK
                     LTTMSLEALADGDLNYKLVRRRRSEA"
     gene            complement(452..1093)
                     /gene="gmk"
                     /locus_tag="VFU45_00970"
     CDS             complement(452..1093)
                     /gene="gmk"
                     /locus_tag="VFU45_00970"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU5357659.1"
                     /translation="MKPFLLVLSSPSGGGKTTIARALLDGREDMGYSVSATTRPMRDG
                     EADGRDYHFLSREEFERRVKAGAFLEWAEYGGNLYGTLRSEIDAILARGSVAVLDIEI
                     RGAAQIRARVPDAVHVFVLPPSAAVLGERLRARRTESSARIRQRLERAADEVMAVPDY
                     DYVVENANLVTAVDQVAAIVEAELHRVSRQERLGERIARLHGEILGEARREQA"
     gene            complement(1090..1953)
                     /locus_tag="VFU45_00975"
     CDS             complement(1090..1953)
                     /locus_tag="VFU45_00975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEU5357660.1"
                     /translation="MTGFGAAEGETSRGTLRVEIRTVNHRYFNLAPKLASDLLPLEGE
                     LRERLRRDFDRGHVAVSARWIGAGDGEVRLAVDMAQARQVVAGLRELQTALGLSGSID
                     LALVARQPDVLVRSAQDPATVTWPEVEPIVARAAQECRAMREREGAALSADILARLDA
                     LEQAAADIARRAPERLVRERDRLRAAVRQLMDGAAPDEARLAQELALSADRLDVTEEL
                     VRLGSHLDACRQALGSSEAIGKRLGFLSQELGREVNTIGSKANDAGIAALVIGMKGEL
                     EKIREQLENLE"
     gene            complement(2140..>2498)
                     /locus_tag="VFU45_00980"
     CDS             complement(2140..>2498)
                     /locus_tag="VFU45_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357661.1"
                     /translation="WYSQATTPAPDGAPPRHWSVTGTIRSRLLHTFRSGAFDLKLELG
                     YEGWRAGVLGQDAGGAPVVLSEAHYLRSLVQVGIESFSVFWESRNLSDEAVAYVPGFR
                     IPRFSGVFGVRWGFMN"
BASE COUNT          365 a          958 c          794 g          381 t
ORIGIN      
        1 aagcgccccg cccggctccc acagggaact caggacgggg cgccccgtca gtgcctcgaa
       61 tgtcagcggc cgcacgaatt ccgcggcggc ccgggtcatc accacatcaa cctggacccc
      121 cgcctcggtc agccgccggg caagaacgca actcttgtag caggcgatcc cgcccgacac
      181 gcccaggacg acgcgccggc cggtccacac cggactcagg cctccgagcg gcggcggcgg
      241 accagcttgt agttcaggtc cccgtccgcc agcgcctcga gcgacatggt ggtcagcttc
      301 tcgtccccga tcgcctcgct ccccttgggg aactcgttca ggtaccgggc gaacttggcg
      361 gccaccagca cgccgagata cttgttgccg ctctgctgcg ccacttcctt cggtgtcacg
      421 atccgcatgc ctgctcctgt tggtcttgcg ttcacgcctg ttcccggcgg gcctcgccga
      481 ggatctcgcc atgcagccgg gcgatccgct cgcccaggcg ttcctggcgc gacacccggt
      541 gcagctccgc ttccacgatc gccgcgacct ggtccaccgc cgtcaccagg ttcgcgttct
      601 cgaccacgta gtcgtaatcg ggcaccgcca tgacttcatc cgccgcgcgc tccagccgct
      661 ggcggatccg ggcggacgac tcggtgcgcc gcgcccgcag ccgctccccc agcaccgccg
      721 ctgacggcgg cagcacgaac acatgcaccg cgtcgggcac ccgggcgcgg atctgggcgg
      781 ccccccggat ctcgatgtcc agcaccgcca ccgagccgcg ggcgagaatc gcgtcaatct
      841 cgctccggag cgtcccgtag agattgccgc cgtactcggc ccattccagg aaggcgcccg
      901 ccttcacgcg ccgctcgaat tcctcgcgcg agaggaagtg gtagtcgcgg ccgtccgcct
      961 ccccgtcccg catcggccgc gtggtggccg acaccgaata gcccatgtcc tcgcgcccgt
     1021 cgagcagcgc ccgcgcgata gtggtcttgc caccccccga gggcgaggac agcacgagca
     1081 ggaacggctt cactcgaggt tctcgagctg ttcgcgaatc ttctcgagct cgcccttcat
     1141 cccgatcacc agcgcggcga tgccggcatc gttggccttg gagccgatgg tgttgacctc
     1201 gcgcccgagc tcctgcgaga gaaagcccag ccgcttcccg atggcctcgc tcgaccccag
     1261 cgcctgccgg caggcatcga ggtggctgcc gagacgcacc agctcctccg tcacgtccag
     1321 ccggtcggcg ctcagcgcca gttcctgcgc cagccgcgcc tcgtcgggtg ccgctccgtc
     1381 catcagctgg cgcaccgccg cacggagccg gtcgcgctcg cggaccagcc gttcgggagc
     1441 gcgccgggca atgtcggccg ccgcctgctc cagggcgtcg aggcgggcca ggatgtcggc
     1501 ggagagcgcg gccccctcac gttcccgcat ggcgcgacat tcctgtgcgg cgcgggccac
     1561 gatcggctcc acctcgggcc atgtcaccgt tgccgggtcc tgggcactcc ggaccagcac
     1621 gtccggctgc cgcgcaacca gggccaggtc gatgctgccg ctcaagccca gcgccgtctg
     1681 gagttcccgg aggccggcca ccacctgccg ggcctgcgcc atgtccaccg ccaggcggac
     1741 ctcgccgtca ccggcaccga tccagcgcgc ggacacggcc acatggcccc ggtcgaagtc
     1801 ccgccggagg cgctcccgca gctcgccttc gagcggcagc aggtcgctcg cgagcttcgg
     1861 cgccaggttg aagtagcggt ggttgaccgt acggatttcg acccggagcg tgccgcggga
     1921 ggtctcgccc tccgccgctc cgaagcccgt catgctgtac ggcaagtcca ctcccccgct
     1981 tcgagaatag cttgggaata tagctcagga tgggcaggat gggcaggata tgggtcgtgc
     2041 gtccttcccg cgcacgcggg aatccatgtt gcccggcctt ctcgaacttg gaccctcgcg
     2101 ttcgcgaggg tgacggtcat cgtggcactc ccgcctgtct cagttcatga atccccaccg
     2161 gaccccgaac acccccgaga atcggggaat cctgaagccg ggcacgtacg ccaccgcctc
     2221 gtcgctcagg ttccggcttt cccagaagac ggagaacgac tcgatcccga cctggacaag
     2281 ggaccggagg tagtgggcct cgctcagcac gaccggcgca ccgccagcgt cctggccgag
     2341 gactccggcg cgccatccct cgtatcccag ttcaagcttc aagtcgaacg ccccgctccg
     2401 gaaggtgtgc agcagccggg accggatcgt gccggtcacc gaccagtgcc gcggtggggc
     2461 tccgtcgggc gcgggcgtgg tggcctgcga gtaccaac
//
LOCUS       DASUAP010000040         5077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_695905, whole genome shotgun sequence.
ACCESSION   DASUAP010000040 DASUAP010000000
VERSION     DASUAP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695905"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1047
                     /gene="argB"
                     /locus_tag="VFU45_00985"
     CDS             112..1047
                     /gene="argB"
                     /locus_tag="VFU45_00985"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU5357662.1"
                     /translation="MSIDSSQGITGLKGALRYVRAYRDHVFVVKLGGDVLRDPAVLDQ
                     VAAQLALLSSLSIRLVVVHGGGPQANELTRRLGGEPKMVAGRRVTDDQALDVVKMVYA
                     GQLNIDLLAALREHEIQAVGLSGVDADLITARRRPPVKVVDDDGRELTVDYGHVGDVT
                     AVDPRVLTTLLDQRFVPVVASLAGDSDGHVFNVNADTVAESLAVALKAQKLVFLTGAP
                     GVLRDRNDPSTLVAFADPDDLAELMASGALAGGMRPKVEACVRAATGGVERTHIIDGR
                     APDALLLEVFTGAGCGTMIVGRKEKATYLGVDLAG"
     gene            1025..2098
                     /locus_tag="VFU45_00990"
     CDS             1025..2098
                     /locus_tag="VFU45_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5357663.1"
                     /translation="MASTSPAEIELLRELVAIPSVSGTEEELGRRVESEARRWGLDAS
                     CDATGVLVEVRGKAAGPTLAFASHLDTVPPGEGWTRDPFTPVIEDGKLYGRGSGDAKA
                     SVAAMLTAARDLAGQGGPARGRLLLLLGFGEETRHTSMPALVARAGSIDAAVVGEPTN
                     LDVAVAQRGLMMVDLIARGDQRHAGYAAEGSFTNAVTTLAEDLLALGAVAQDRPHPVL
                     GQPTITATMVEAGVSRNVTPPSARAVLDVRSTPAWSHGELADALRARLRSEVVVTSER
                     LVPCETPSGSALLAAVLRARPAARTFGSPTCSDWVFLRGSDAVKCGPGTSRRSHTADE
                     SVDLAEVTEARSFYARVAKEYLR"
     gene            2095..3507
                     /locus_tag="VFU45_00995"
     CDS             2095..3507
                     /locus_tag="VFU45_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEU5357664.1"
                     /translation="MKSRRVSGSAGRRGRTPATLWSPSGGIDALMFAYTVGDDREWDS
                     ALLAWDIYGSLGHLEGMRDAGLVSRRDYAALRRGLRSALVAARRGRLAIGAEHEDAHS
                     AVEAWLTRRLGEAGERIHTGRSRNDQVATDLRLFLKDRLLAIHRSGQAAAEALIRFAG
                     RHRTALWPGYTHTRRAMPSSAALWAAAHAEGIVDTLEGLPALWRRVDRCPLGSAAGYG
                     APLPIRREVAARALGFRAVEPNVAAVQNGRGKLEAAALFWCTQLGHDLAKLSADVILY
                     TGEEFGLLRLPASLATGSSIMPHKRNPDLFELTRARAAALEGDLFATMAIGGRLPSGY
                     HRDFQLLKEPLIRGLRRTEEMLVMTAHAAGGLEVDRARGAALLEGGALATDEVMRRVE
                     SGTPFRRAYREVAATLKHGESFPAPAPREIVARRRATGGVGNVGLQALRTRIGAAARW
                     ARAEERRFRGALTRLDGSGA"
     gene            3504..3959
                     /locus_tag="VFU45_01000"
     CDS             3504..3959
                     /locus_tag="VFU45_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357665.1"
                     /translation="MTSDRRKRHLKILELISARRIGTQEELADALSAEGWEVTQSSVS
                     RDIASLGLVKVEGAYQRARRALKPRTDPDEQRIAEGLLAVDPAGEALVVIHTPPGEAN
                     RVAVALDRLAWPEFMGTIAGDDTIFVAVRDGKAQRDFIRRLKRLAGPTD"
     gene            3976..4341
                     /locus_tag="VFU45_01005"
     CDS             3976..4341
                     /locus_tag="VFU45_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357666.1"
                     /translation="MARSEIITHAGKRVAVLDFSGILGEQDGLAAIAEAGKLVQAQPP
                     KSVYTLTDVTGARFNAATLAALKALASDNAPYVRAGAVFGMSTLHKAAYLTVMYFSRR
                     QIPAFDTKEQALAWIEKQE"
     gene            4509..5012
                     /locus_tag="VFU45_01010"
     CDS             4509..5012
                     /locus_tag="VFU45_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5357667.1"
                     /translation="MAIKDTLLPEFDQEIALTRKMLERVPEDALDWQPHAKSMTLGRL
                     ATHLAETVAWGERALTSDSFDVLPPGSPPPVRKALPSRAAMLELLDRNAARSRAAIAS
                     TDDAAFGRPWSLLRGGATVFTLPRIGIVRTMLLSHLIHHRGQFSVYLRMRDVPVPGMY
                     GPSADER"
BASE COUNT          735 a         1741 c         1888 g          713 t
ORIGIN      
        1 gtaagaggtg aaacgtatga ggagaaggga aatcgctaac ttcgcgctga tagctgatag
       61 ctgatagctg atagccgaag gatggcattg aggacaccgc acgggggatc gatgagcatc
      121 gacagttccc agggcatcac cggactcaag ggcgcgctcc gctacgtgcg ggcctaccgc
      181 gaccacgtat tcgtcgtcaa gctcggcggc gatgtcctgc gcgacccggc ggtgctcgac
      241 caggtggccg cccagctggc gctcctctcc tcgctcagca tccgcctggt ggtggtgcac
      301 ggtggcgggc cgcaggccaa tgagctgacc cggcggctcg gtggggaacc gaagatggtg
      361 gcgggccggc gcgtcaccga cgaccaggcg ctcgatgtcg tcaagatggt ctacgccggc
      421 cagctcaaca tcgacctcct ggccgcgctg cgggaacacg agatccaggc ggtcgggctc
      481 tccggcgtgg acgccgacct catcaccgcc cgccgccgcc cgccggtcaa ggtggtggac
      541 gacgacggcc gcgagctcac cgtcgactac ggccacgtgg gcgacgtgac cgccgtcgac
      601 ccgcgcgtcc tcaccacgct cctcgaccag cgcttcgtac cggtggtggc gagcctggcc
      661 ggcgacagcg acgggcacgt cttcaacgtg aacgccgaca ccgtggcgga atcgctcgcg
      721 gtggcgctca aggcgcagaa gctggtgttc ctgaccggcg cccccggcgt cctccgcgac
      781 cggaacgacc cttcgacgct ggtcgcgttc gccgaccccg acgacctggc cgagctgatg
      841 gcgagcggcg cgctggcggg ggggatgcgc cccaaggtgg aggcgtgcgt ccgtgcggcc
      901 accggcgggg tcgagcgcac gcacatcatc gacggacgcg cgcctgacgc gctcctcctc
      961 gaggtgttca ccggtgcggg gtgcggcacc atgatcgtgg gccggaagga gaaggccact
     1021 taccttggcg tcgacctcgc cggctgagat cgagctgctc cgcgagctcg tcgcgatccc
     1081 ctcggtgagc ggtaccgagg aggagctggg gcgccgggtg gaatcggagg cccggcgctg
     1141 ggggctcgac gcctcctgcg atgcgaccgg cgtgctggtc gaggtgcgtg ggaaggcagc
     1201 gggacccacg ctcgccttcg cctcgcatct cgacacggtg ccaccgggag agggatggac
     1261 gcgcgacccc ttcaccccgg tcatcgagga cggcaagctc tacgggcgcg gctcgggcga
     1321 cgccaaggcg agcgtcgccg cgatgctcac cgcggcgcgg gacctggccg ggcagggcgg
     1381 cccggcgcga ggacggctcc tcctccttct cggcttcggc gaggagacgc gacacacgtc
     1441 gatgccggcg ctggtggcgc gggcggggag catcgacgcc gcggtggtgg gggagccgac
     1501 caatctcgac gtcgcggtgg cgcagcgcgg cctcatgatg gtggacctca tcgcccgcgg
     1561 cgaccagcgc cacgccggct acgccgctga gggcagcttc accaacgcgg tgacgacgct
     1621 ggccgaggac ctgctggcgc tcggcgcggt ggcgcaggac cggcctcatc cggtgctggg
     1681 acagcccacc atcaccgcca cgatggtgga ggcgggggtg agccggaacg tgacgccccc
     1741 ttccgccagg gcggtgctcg acgtcaggag cacgccggcg tggtcgcacg gcgaacttgc
     1801 cgatgcgctc cgggcccggc tccgatcgga agtcgtcgtg acgagcgaac ggctcgtgcc
     1861 ctgcgaaacg ccgtccggtt cggcgctcct cgcggcggtg ctgcgggccc ggcccgccgc
     1921 gcgaacgttc ggcagcccga cctgttcgga ttgggtcttc ctccgcggtt cggacgcggt
     1981 caagtgcggg cccgggacca gccggcggtc gcataccgcc gacgaatcgg tcgacctcgc
     2041 cgaagtcacc gaagcacggt cgttctacgc gcgggtcgcg aaggagtacc tgcgatgaag
     2101 tccaggcggg tcagcggatc ggcgggccgg cggggccgga cgccggcgac gttgtggtcg
     2161 ccgtcggggg gcatcgatgc gctgatgttc gcctatacgg tcggcgacga ccgcgaatgg
     2221 gactccgcac tcctcgcatg ggacatctac gggagcctcg gacacctcga gggcatgcgg
     2281 gatgccgggc tggtctcgcg ccgcgactac gccgcgctcc ggcgcgggct tcgctcggca
     2341 ctcgttgcgg cacgacgcgg ccggctcgcg atcggcgcgg agcacgagga cgcccacagc
     2401 gcggtggagg cgtggctcac ccgccggctg ggcgaggccg gcgagcggat ccataccggc
     2461 cgctcccgga acgaccaggt ggcgaccgac ctccggctct tcctcaagga ccgcctcctc
     2521 gcgatccacc gatcgggtca ggcggcggcc gaagcgctga tccggttcgc ggggcggcac
     2581 cggacggcgc tctggcccgg ctacacccac acccggcgag ctatgccatc gtcggcagcg
     2641 ctctgggccg cggcgcatgc cgaagggatc gtggacacgc tcgagggact gccggcgctc
     2701 tggcgccggg tggaccggtg cccgctcggg agcgccgcgg ggtacggcgc gccgctcccc
     2761 atccgccgcg aggtggcggc gcgcgccctc ggcttccgcg cggtcgagcc caatgtggcg
     2821 gcggtgcaga atgggcgcgg caagctcgag gcggcggcgc tcttctggtg cacccagctc
     2881 gggcacgacc tcgccaagct ctccgccgac gtgatcctct acaccggcga ggagttcggt
     2941 ctcctccggc ttccggcgtc gctcgcgacc gggtcgagca tcatgccgca caagcggaac
     3001 ccggacctct tcgagctcac ccgcgcgcgg gcggcggcgc tcgagggcga cctctttgcg
     3061 acgatggcga tcggcggccg gctgccgagc gggtatcacc gcgatttcca gctcctcaag
     3121 gagccgctga tccggggcct ccgccgtacc gaggagatgc ttgtgatgac ggcgcacgcc
     3181 gcgggcgggc tcgaggtcga ccgcgcgcgg ggggcggcgc tccttgaagg gggtgcgctc
     3241 gccaccgacg aagtgatgcg gcgggtcgag tcggggaccc ccttccgccg ggcgtaccgt
     3301 gaggtcgccg cgacgctcaa gcacggggaa tcctttccgg cgcccgcgcc ccgcgagatc
     3361 gttgcgcgcc ggcgtgcgac cggcggcgtc ggcaacgtgg gcctgcaggc gctccggaca
     3421 cggatcggtg cggcggcgcg ctgggcccgg gcggaggagc gccggttccg gggcgcgctc
     3481 acccggctcg acgggagcgg cgcgtgacgt ccgaccgccg caagcgccac ctcaagatcc
     3541 tggagctcat cagcgccagg cggatcggca cgcaggaaga acttgccgac gcgctgagcg
     3601 ccgaggggtg ggaggtgacg cagtcgtcgg tgagccggga tatcgcctcg ctggggctcg
     3661 tcaaggtgga aggcgcctac cagcgcgcgc ggcgggcgct caagccccgc accgacccgg
     3721 acgagcagcg cattgcggag gggctgcttg cggtcgaccc cgcgggtgag gcgctggtcg
     3781 tgatccacac gccccctggg gaggcgaacc gggtggcagt ggcgctcgac cggctggcgt
     3841 ggccggaatt catgggtact attgcggggg acgacacgat cttcgtggcg gtgcgcgacg
     3901 gcaaggcgca gcgggacttc atccggcgcc tgaagcggct tgctggcccg acggactgaa
     3961 cccgcggagg atccgatggc tcggagcgag atcatcaccc atgccgggaa gcgggtggcc
     4021 gtgctcgact tctccgggat cctgggggaa caggacggcc tcgcggccat cgcggaggca
     4081 ggcaagttgg tgcaggcgca gccgcccaag tcggtctaca ccctcaccga cgtgaccggc
     4141 gcccggttca acgccgccac cctggcggcg ctcaaggcgc tcgcatcgga caatgcaccg
     4201 tatgtcaggg ccggcgccgt gttcgggatg agcaccctcc acaaggccgc ctacctcacg
     4261 gtgatgtact tctcccgccg ccagatcccc gcgttcgaca ccaaggagca ggcgctggcg
     4321 tggatcgaga agcaggagta agtggtgaga cgtgagcggt gagacgtgag agggaagagg
     4381 taagcggtga gcggtgagag gtgagagggc cccggtcgag atctcgaagg ggagcccttt
     4441 tcctccttcc ccctttcatt ttccctctca cctcttacct ctcacgtctc accttcagga
     4501 gcgctcggat ggccatcaag gacacgctgc tgccggagtt cgaccaggag atcgccctga
     4561 cccgcaagat gctcgagcgg gtgccggagg acgcgctcga ttggcagccc cacgccaagt
     4621 cgatgacgct gggccggctc gccacccacc tggcggagac ggtggcgtgg ggggagcggg
     4681 cgctcacctc ggactcgttc gacgtcctgc cgccgggttc gccgccgccg gtgcggaagg
     4741 cgctgccttc ccgcgcggcg atgctcgagc ttctcgaccg gaacgcggcc cggtcgcggg
     4801 cggccatcgc gtccaccgac gacgcggcgt tcggccggcc gtggagcctc ctccgcggcg
     4861 gtgccaccgt cttcaccctg ccgcgcatcg ggatcgtccg cacgatgttg ctgagccacc
     4921 tgatccacca tcggggccag ttcagcgtct acctgcggat gcgggacgtg ccggtgccgg
     4981 ggatgtacgg gccgagcgcg gacgagcggt agtcagacgc cgtccccgtc cgcccgggga
     5041 ttgaagaaga ggtcgaacgc gaggaaggct gtcttcg
//
LOCUS       DASUAP010000041         5834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1009490, whole genome shotgun sequence.
ACCESSION   DASUAP010000041 DASUAP010000000
VERSION     DASUAP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009490"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1379)
                     /gene="hemG"
                     /locus_tag="VFU45_01015"
     CDS             complement(36..1379)
                     /gene="hemG"
                     /locus_tag="VFU45_01015"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEU5357668.1"
                     /translation="MRRLVVIGGGISGLAAAWAAQRAGGVEVLVLERAPQVGGKANSI
                     VRDGWLVEGGPSGYLGGRPELERLIDEVGLSGERVPANSASARRFLFRAGRMRRLIAN
                     PVGLLTEGVLGPAGVARMLAEPVIPRRKDGADESVWDFAARRLGSDVADRMILPMALG
                     IFAGDARRLSLAAAFPRMAALEREHGSLIRGMIARRKGPGPGGLSSFRLGMQQLPRTL
                     AERGGFTVRCNASVNGLARAGTEWLVAVAGDREPIAADGVILAGEPWSVAPLLVPHDE
                     RLAAELTAIPCPPVAVVALGYAPAAAARIPTGFGVLIARGEGFRMLGNLWEAQLYPGR
                     GPAEHALVRTMYGGAVDPEAGALSEGELVALARKEVGQLFGISEAPVMEQVVRWPRAI
                     PQYEIGHSARVKRIEAAVAALPGLAITGNGLRGVSFTDAATDGLRTGEALARSLV"
     gene            complement(1381..2814)
                     /gene="hemN"
                     /locus_tag="VFU45_01020"
     CDS             complement(1381..2814)
                     /gene="hemN"
                     /locus_tag="VFU45_01020"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU5357669.1"
                     /translation="MDPKHPASAASGAERPVGREVTAELLARHDRPGPRYTSYPTAVE
                     FNEGIGTAEYLVRLAEADKLGDAPLSLYLHLPFCEQRCLFCGCHVIITKHRDVAEPYL
                     ELVKREIDLLAERLPNRRRFAQLHLGGGTPTYFSPAQLTDLLSHLLQRFHPSKGAELA
                     LEADPRVTSAAHIDALADLGFNRISFGVQDLTPVVQEAINRVQSLEQTAALVEQARRR
                     GFRGINVDLIYGLPLQTPESFERTVDSVIGLGIDRAAVYSFAFVPWVRGHQKKIEEDQ
                     LPDAATKVALFAIARERFLKAGYEPIGMDHFARPDDELARAKHEGRLRRNFQGYAVIP
                     GDDVLGIGISAIGDVRGAYIQNEKKLSTYEARIKAGELPVTRGVPRSADDEVRRTVIH
                     ELMCNFRVDTGDISRRYGIDFASYFAEDLALLAEPEREGLVRIGNERIEATPVGELFV
                     RNLAMCFDRYQREKHEGEAKPVFSRTV"
     gene            complement(2814..3788)
                     /gene="hemH"
                     /locus_tag="VFU45_01025"
     CDS             complement(2814..3788)
                     /gene="hemH"
                     /locus_tag="VFU45_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU5357670.1"
                     /translation="MPVTHLVLVNLGTPEAPEPASVREFLDEFLGDPAVVDLPRWFWL
                     PILRGIILRRRPARVAEQYRSIWTDGGSPLRVATERIVADVRARAAGRFGVSSAYRYG
                     EPSLDTVMQRLARDESGPIVVSPLFPHRTDATTGTAFLRAREAAERAGIAHRLVERLV
                     DPAAPGYINAMAARWRRDLDSAGHDVEHLVISFHGIPVRYDRRERGVYVRDCRATTDA
                     FLKAIAWPAERATLAFQSKFGPEPWLKPTTAGLLEELPRRGVRRVAVITPGFVTEGLE
                     TVEEIGIRGRESFIEAGGTHFLRIGSVELEPEFLDELAELATTSNGRA"
     gene            complement(4138..4593)
                     /locus_tag="VFU45_01030"
     CDS             complement(4138..4593)
                     /locus_tag="VFU45_01030"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU5357671.1"
                     /translation="MPLNAGDTAPDFTLPDQRGTPVSLGAFRGRRTVVLYFYPRDETR
                     GCTAEACRFRDDFEVFQEAGAEVIGVSSDSVKSHAEFAANHRLPFTLLADEDGAVRKR
                     YGVERQLLVVPGRTTFIIDRQGIIRHVFSQLFDATKHVDEALSVVRSLG"
     gene            complement(4805..>5834)
                     /locus_tag="VFU45_01035"
     CDS             complement(4805..>5834)
                     /locus_tag="VFU45_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5357672.1"
                     /translation="IYYVGTEASPLERQLYAVRFDGSHARRLTTATGTHSIDMSPSTR
                     YYIDRWSSVSTPRQVELWATGGKMLKKIEDNAKVTAWVASHEYSPTKLFNFTTSDGVK
                     LDGSMVLPVPFDSTRRYPVVFAVYGGPGSQQVYNSWGGGGYGQWLAQHGYIVVGLNNR
                     GTNNYGSAFMKVVYKHLGEWESHDFAEAARYLATLPYVDGKRVAIMGTSYGGYSTVYT
                     MAKYPDLFTVGVANSAVTDWRLYDDIYTERYMSLPSDNAAGYDSSSAVVLAPRLTGHL
                     LLIHSMMDDNVHPQNTMQFLTAMANAGHDVDLRIYPPGHHGAAYNFQSFLLIQQATDE
                     WLGRWLKP"
BASE COUNT          853 a         2023 c         2100 g          858 t
ORIGIN      
        1 cgcgcggcgg agccggcggc aactccgacg gaagactaga ccaggctgcg cgccagcgcc
       61 tccccggtcc ggagcccgtc ggtggcggcg tcggtgaacg acaccccccg gagcccgttc
      121 ccggtgatgg cgagccccgg aagcgccgcg accgcggcct cgatccgctt gacccgggcg
      181 gagtgaccga tctcgtattg cgggatggcc cggggccagc gcaccacctg ctccatcacc
      241 ggcgcttcgg agatcccgaa gagctgaccc acttccttcc gcgccagcgc cacaagctct
      301 ccctcggaga gcgcgcccgc ctccggatcc accgcgccgc cgtacatcgt gcggaccagc
      361 gcgtgttcgg cgggccctcg accgggatag agctgcgcct cccagaggtt gccgagcatc
      421 cggaaccctt ccccccgggc gatgagcacg ccgaatcccg tcgggatgcg agccgcggcc
      481 gcgggagcgt agccgagcgc cacgaccgcg acgggagggc aggggatggc ggtgagctct
      541 gccgccaggc gctcgtcgtg tggcaccagg aggggcgcca ccgaccaggg ctcgccggcc
      601 aggatcacgc catcggcggc aatcggctca cggtctcccg ccacggcgac aagccattcg
      661 gtccccgcac gtgcgagccc attgacgctc gcgttgcagc gcacggtgaa cccaccccgt
      721 tcagcgagtg tgcgggggag ctgctgcatg ccgagcctga aggaggagag tccgccgggg
      781 ccgggtccct tccgccgggc gatcatcccg cggatcaggg aaccgtgctc ccgctcgagc
      841 gccgccatcc gggggaacgc cgccgcgagc gagagccgtc gcgcgtcgcc ggcgaagatg
      901 ccgagcgcca tcgggaggat catccggtcg gcgacgtcgc tgcccaggcg gcgggcggcg
      961 aagtcccaga ccgactcgtc ggcgccgtcc ttgcgccggg ggatgaccgg ctcggccagc
     1021 atccgcgcca caccggcggg gcccagcacc ccttcggtga ggagccccac cgggttggcg
     1081 atgagccgcc gcatccggcc ggcgcggaag aggaagcggc gggcggatgc cgagttggcc
     1141 ggcacccgct caccgctgag accgacctcg tcgatgagtc gttcgagctc tggccgcccg
     1201 cccaagtagc ccgacgggcc cccctccacc agccagccgt cgcgcacgat gctgttcgcc
     1261 ttgccgccga cctgcggggc gcgctccagc accagcacct cgacaccgcc ggcgcgctgt
     1321 gccgcccacg ccgccgccag ccccgagatc ccgccgccga tgacgacgag gcgtcgcacg
     1381 ctataccgtg cggctgaaga ccggcttcgc ctcgccctcg tgcttttccc gctggtagcg
     1441 gtcgaagcac atcgccaggt tgcgcacgaa gagctcgccc accggcgtgg cctcgatgcg
     1501 ctcgttgccg atcctgacga gcccttcccg ctcgggttcc gccaggagcg ccaggtcctc
     1561 cgcgaagtag ctcgcgaagt cgatcccgta ccgccgcgag atgtcgccgg tgtcgacccg
     1621 gaagttgcac atcagttcgt ggatcactgt gcggcggacc tcgtcgtcgg cgctccgggg
     1681 gacgccgcgc gtcaccggga gttcgcccgc cttgatgcgc gcctcgtagg tcgagagctt
     1741 cttctcgttc tggatgtagg cgccccgcac gtcgccgatg gcggagatgc cgatgccgag
     1801 gacgtcgtcg ccggggatga cggcatagcc ctggaagttc cgccgcagac gcccttcgtg
     1861 cttggcgcgg gcgagttcgt cgtcggggcg ggcgaagtgg tccatgccga tgggctcgta
     1921 ccccgccttg aggaagcgct cccgcgcgat ggcgaagagc gccaccttgg tggcggcatc
     1981 ggggagctgg tcctcctcga tcttcttctg gtgcccgcgc acccacggca cgaaggcgaa
     2041 tgagtacacc gccgcgcggt cgatgccgag cccgatgacc gagtccacgg tgcgttcgaa
     2101 gctctccggc gtctggagcg ggagcccgta gatgaggtcg acgttgatgc cgcggaaccc
     2161 ccgccggcgg gcctgttcca ccagcgccgc ggtctgctcc agcgactgca cccggttgat
     2221 cgcctcctgc accacggggg tcaggtcctg gacgccgaag gagatccggt tgaagccgag
     2281 gtcggcgagg gcgtcgatgt gggcggcgga ggtgacccgg ggatcggcct cgagcgccag
     2341 ctccgccccc ttcgacggat ggaatcgctg gagcaggtgg ctcagcaggt cggtgagctg
     2401 cgccggggag aagtaggtcg gcgtcccccc gcccaggtgc agctgcgcaa agcgccggcg
     2461 attcggcagc cgctcggcca gaaggtcgat ctcgcgcttg acgagctcga ggtatggctc
     2521 ggcaacgtcg cggtgcttgg tgatgatcac gtggcagccg cagaagagac agcgctgctc
     2581 gcagaacggc aggtgcaggt agagcgagag cggggcatcg cccagcttgt ccgcctccgc
     2641 cagccgcacc aggtactccg cggtgccgat cccttcgttg aactcgacgg cggtcgggta
     2701 gctggtgtag cgcggcccgg gccggtcgtg gcgggccagc agctcggcgg tgacctcgcg
     2761 ccccaccggc ctctcggcgc cgctcgccgc ggatgcagga tgcttcggat ccattacgcc
     2821 ctcccgttcg acgtcgtggc gagctccgcc agctcgtcga ggaattccgg ctccagctcc
     2881 accgacccga tgcggaggaa gtgggtgccc cccgcctcga tgaacgactc ccgcccccgg
     2941 atcccgatct cctcgaccgt ctccaatccc tcggtcacga accccggagt gatgaccgcg
     3001 acccggcgga cgccgcggcg cggcagttct tccaggaggc cggcggtggt gggcttgagc
     3061 cacggctccg ggccgaactt cgactggaag gcgagggtcg cgcgttcggc cggccaggcg
     3121 atcgccttga ggaaggcgtc ggtcgtcgcg cggcagtcgc ggacgtagac gccccgttcg
     3181 cggcggtcgt agcgcaccgg gatcccgtgg aacgaaatga caaggtgctc gacgtcgtgc
     3241 cccgccgagt cgaggtcgcg gcgccagcgg gcggccatgg cgttgatgta gcccggcgcc
     3301 gccgggtcca ccaggcgctc caccagccgg tgggcgatcc ccgcgcgctc ggcggcctcg
     3361 cgggcgcgca ggaaggcggt gccggtggtg gcgtcggtgc ggtggggaaa gagggggctc
     3421 acgacgatcg gtcccgattc atcccgggcc agtcgctgca tcaccgtgtc gagggagggt
     3481 tcgccgtagc ggtacgccga gctcacgccg aagcgtcctg ccgcgcgggc gcggacgtcg
     3541 gccacgatcc gttccgtggc cacgcggagg ggggagccgc cgtcggtcca gatggagcgg
     3601 tactgttccg ccacccgggc cgggcggcgg cggaggatga tcccgcggag gatggggagc
     3661 cagaaccagc gggggaggtc gacgaccgcc gggtcgccca ggaattcgtc caggaactcg
     3721 cgcaccgagg ccggctccgg cgcctcgggg gtccccagat tgaccagcac cagatgtgtc
     3781 accggcaccc acggcagctg agtggtcgtg aagtcaccga ttgaatgaac aacggccggg
     3841 ccgatccggc ttccgtatat ccccggacgg gcgggaatgc agcaatggcg cggcgcaggg
     3901 cctatgggag tcgcatggat ccgcaggggc ggcgcatgcg ccgcccgccg gcgtgggggt
     3961 cgctcattca ggcggggggg tcgccggcgg cgacccgacg ctccgcggtg acgatgggcc
     4021 cgcaatgggg tggccgcagg ccacccctta caccgccgtc ttgcccatcg aatttcgggt
     4081 cgccgcaggc gacccccaca actgcagact gtcgggtggc cgcaggccac cgcctactca
     4141 ccccagcgac cgcaccacgc tgagcgcctc gtccacgtgc ttcgtggcgt cgaagagctg
     4201 ggaaaagacg tgccggatga tgccctggcg gtcaatgatg aacgtggtcc gtcccggcac
     4261 cacgagcagc tgccgctcga cgccgtagcg cttccgcacg gcaccgtcct catcggccag
     4321 gagggtgaac ggcaggcggt ggttggcggc gaactccgcg tgcgacttga ccgagtccga
     4381 gctcacaccg atcacctcgg cgcccgcctc ctggaacacc tcgaagtcgt cgcggaagcg
     4441 gcacgcctcg gcggtgcagc cgcgagtctc gtccctgggg tagaagtaga ggaccacggt
     4501 ccgccggccc ctgaatgcgc cgagcgacac cggcgtgccg cgctggtcgg ggagggtgaa
     4561 gtcgggggcg gtgtcgccgg cgttgagggg cattcggggt gcctggtcga ggtgggatcg
     4621 tgggggtcgc gcactcaggg gtgggggtcg cctgcggcga cccgacacct cgcggtgacg
     4681 aggagtggcc tgcggccacc tcctacatcg ccacgttgcc catcaaatgt cgggtcgccg
     4741 caggcgaccc ccacgccaca aggtggtcgg gtggccgcag gccaccccca cgggcgctgg
     4801 acccctacgg cttgagccac cgtcccagcc actcgtcggt ggcctgctgg atcaggagga
     4861 agctctggaa gttgtacgcc gcgccgtggt gccctggcgg gtagatccgg aggtccacgt
     4921 cgtggccggc gttggccatc gcggtcagga actgcatcgt gttctgcgga tggacgttgt
     4981 cgtccatcat cgagtggatg agcagcaggt gcccggtgag ccgcggcgcc aggaccaccg
     5041 ccgagctcga gtcgtacccc gccgcgttat cggaggggag gctcatgtag cgctcggtgt
     5101 agatgtcgtc gtagagccgc cagtcggtca cggccgagtt ggcgacgccg acggtgaaga
     5161 ggtccgggta cttcgccatc gtatacaccg tgctgtagcc gccgtagctc gtgcccatga
     5221 tggccacgcg cttcccgtcg acgtacggca gggtggccag gtaccgcgcc gcctcggcga
     5281 agtcgtggct ctcccactcg cccaggtgct tgtacaccac cttcatgaac gcgctgccgt
     5341 agttgttggt gccgcggttg ttgagcccca ccacgatgta gccgtgctgg gccagccact
     5401 ggccgtaccc cccgccgccc caggagttgt acacctgctg ggagccgggg ccgccgtaca
     5461 ccgcgaagac caccgggtag cgccgggtcg agtcgaacgg caccggcagc accatcgaac
     5521 cgtcgagctt gacgccgtcg gaggtggtga agttgaagag cttggtgggg gagtactcgt
     5581 gtgatgccac ccacgccgtc accttggcat tgtcctcgat cttcttgagc atcttgccgc
     5641 cggtggccca gagctccacc tggcgcgggg tgctcaccga cgaccagcgg tcgatgtagt
     5701 accgggtgct gggcgacatg tcgatcgaat gggtgccggt cgcggtggtc aggcgccggg
     5761 cgtggctgcc gtcgaaccgg acggcgtaga gctgtcgctc cagcggagag gcctcggtgc
     5821 cgacgtagta gata
//
LOCUS       DASUAP010000042         6463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_314571, whole genome shotgun sequence.
ACCESSION   DASUAP010000042 DASUAP010000000
VERSION     DASUAP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314571"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFU45_01040"
     CDS             <1..493
                     /locus_tag="VFU45_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEU5357673.1"
                     /translation="GEDAPVPVRPVTVTPGSRSCGELLLEMQRERRHLAVVLDEFGGT
                     LGIATLEDLLEALVGEIFDELDGPGGPGSATTGRPAPLEADAATPLADVIARFGVTLP
                     VTTATTVAGLVAELARRIPEAGERFTLRGLEFDVLQASPARAERILVRAAGGATSLDR
                     ETP"
     gene            490..1833
                     /gene="mgtE"
                     /locus_tag="VFU45_01045"
     CDS             490..1833
                     /gene="mgtE"
                     /locus_tag="VFU45_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU5357674.1"
                     /translation="MSQPGVPFDELTALVRAGRIREFQERARGLDPADLADVFAALDE
                     EERLAAVRTLPARLSGQALVMMPEDEHAEETLAALAPTEAAGIVGTLEDDDAADILGE
                     MAPDEQRRILDQVGAEDRAEFQSLLKYGKETAGGLMTARVMTVRDIDTAAEAIEEIRG
                     QSEETGEFYQVFVVDGTGKLQGVLPLRDLVTSRADRPVREFMEAVVHSVRPEEDQEAV
                     ARLMSRYNLPSVPVIDQEGRLLGRVTFDDVIDVVEAEGTEDMLKFGGTSADEEVAGTW
                     WQAVKSRLPWLYVNLATAFLAGGVVKIFLDTVNRLTDLAIWMPIIAGMGGNAGTQALA
                     VSVRGLALGLLTMKEFWRVIGKEMLVGLVNGAANGAVSALVALLIHETPMLGVCVFLS
                     MTGNLFVAGFAGAFIPMILKRLNVDPAIASSIFVTTFTDVCGFLMLFMTARALIL"
     gene            1910..2944
                     /gene="meaB"
                     /locus_tag="VFU45_01050"
     CDS             1910..2944
                     /gene="meaB"
                     /locus_tag="VFU45_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU5357675.1"
                     /translation="MSALASEITSGNTPALARAISVVENGRPGFEELLVGLHPRLGRA
                     RRIGITGPPGAGKSTLTERLILALRAQGHKVAVVAVDPTSPFTGGALLGDRIRMESVA
                     LDPDVYIRSMATRGSLGGLATSTREVCDVLDAAGFDRILIETVGVGQSELAVTRSADT
                     TVLVLVPESGDGIQALKSGVMEAADVYVVNKADRPGADRLRQEIEVSLGIRSGHGFRH
                     VPAHHGALLSARRVRAEAGDPAADAWLPPVLATVAAAGDGVDDLVTALDRHAEWLERS
                     GELDERRRRRISDRIREVVARHLQQWLWDETQADRMVRERLDEVAAGRQSPYEVAAEV
                     LDQVRQGARV"
     gene            2962..4656
                     /locus_tag="VFU45_01055"
     CDS             2962..4656
                     /locus_tag="VFU45_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5357676.1"
                     /translation="MAKGHLEAELARKDRELERLRAEVDAWRRVAGALPVRDDSDFTT
                     ISGHAVEPLYTALDLGDAGAAAVGLPGEFPYTRGIHHTGYRGKLWTMRQFAGFGTAED
                     TNRRYKFLLERGQTGLSVAFDFPTLMGYDSDHPRSEGEVGKCGVAISSLADMETLFDG
                     IPLDRVSVSMTINGPAAMLFCFFVAAAEKQGIPIEKLQGTIQNDILKEYMAQHAWIYP
                     AEPALKVIVDLFEWGSVHTPKWNTISISGYHIREAGATAAQELAFTLADGFCYVEHGV
                     ARGLDVDAFAPRLSFFWDVHNDFFEEIAKLRAARRIWARVLRDKYGAKDPRSWKMRFH
                     CQTAGVSLTAQQPMNNVVRVAYQALAAVLGGTQSLHTNALDETLALPTEQSVRVALRT
                     QQILAYETGVPNTHDPLGGSWFVEALTDQMEREAMGLFAEIDAQGGVVAAIDSGWFQR
                     QIARSAARFQSEVEAGSKVVVGLNRFEEAEEAAPEILKISDQAERQQRERMARMRAGR
                     DQALVDRRLEALRSAAVADQNVIPAMLDCARAYCTLFEIRHVLENVYGSYREPVFF"
     gene            4667..5830
                     /locus_tag="VFU45_01060"
     CDS             4667..5830
                     /locus_tag="VFU45_01060"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357677.1"
                     /translation="MPSPIARQIERLARERSGPPVVSCYLKLEPRDRARGKYLIKLKN
                     RLKAAEQALERRDFSRVEREGARADLERLREYLGSHDRLPPTQGIAVFVSGARKLFEA
                     IPLPSVHRSRLSVERVPLVRELAGVEDEIGRLLVVVADRASARFFQVTAFGAEPVGSP
                     VLADDSAPARPRPRSGRRVSSRVGEHAYHNRIRSVQQRHWEAVAQQLFLLDRTEPARA
                     IVILGQGPAAAAIGPFLHPYLAERVIGHGTLSPREATPSAVYAAALDVRHEWERARER
                     ADVARMRERVGRGWSTNGVVPTLKALATGQVRTLLVHGDATVPGYRLAGSGRLVIPGK
                     SEAWRGEGEPEPVADVIDEALEEALRQRIDLDVLYDAEAARGVDGLAAILRFK"
     gene            5877..6323
                     /locus_tag="VFU45_01065"
     CDS             5877..6323
                     /locus_tag="VFU45_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU5357678.1"
                     /translation="MMMMAPQQAPTATHKRPIRVLVAKPGLDGHDRGAKVVAAALRDA
                     GMEVIYTGLHQTPEMIARAAVQEDADVVGLSILSGAHMTLFPRVKQLLDAEGRGDVLL
                     TGGGIIPQDDIQQLEAMGVGRLFGPGTPTTSLVAYIQEWAAAHLRD"
BASE COUNT          915 a         2342 c         2306 g          900 t
ORIGIN      
        1 cggcgaggat gcgccggtcc cggtgcgccc ggtgaccgtg acgccgggga gccgcagctg
       61 cggcgagctc ctgctggaga tgcagcgcga acgccgccac ctcgccgtcg tcctcgacga
      121 attcggcggc accctcggga tcgcgacgct cgaggacctg ctcgaggcgc tggtcggcga
      181 gatcttcgac gaactcgacg gccccggagg tccgggatcg gccacgacgg gccgtcctgc
      241 gccgctcgag gccgacgcgg cgacgcccct cgccgacgtg atcgcccggt tcggcgtgac
      301 gctcccggtc accaccgcca cgaccgtcgc cgggctcgtg gcggagctgg cccgccggat
      361 cccggaggcc ggggagcgct tcaccctgcg ggggctcgag ttcgatgtcc tgcaggcctc
      421 gcccgcgcgc gccgagcgga tcctcgtccg ggccgcgggt ggcgcgacca gcctcgatcg
      481 ggagacgcca tgagccagcc cggggtcccg ttcgatgaac tgacggcgct cgtccgcgcg
      541 gggcggatcc gcgagttcca ggagcgcgcg cgggggctcg atcccgccga cctggccgac
      601 gtcttcgccg cgctcgacga ggaggagcgc ctcgcggcgg tccggaccct gccggcccgg
      661 ctctccgggc aggcgctggt gatgatgccc gaggacgagc acgcggagga aacgctcgcc
      721 gcgcttgcac cgacggaggc ggcggggatc gtcggcacgc tcgaggacga cgacgcggcc
      781 gatatcctgg gcgagatggc gcccgacgag cagcgtcgga tcctcgacca ggtcggcgcc
      841 gaggatcgcg ccgagttcca gtccctcctc aagtacggca aggagaccgc cggcgggctg
      901 atgaccgcac gggtcatgac cgtgcgcgac atcgacaccg ccgccgaggc catcgaggag
      961 atccgcgggc agtccgagga gacgggcgag ttctaccagg tgttcgtggt cgacgggacg
     1021 ggaaagcttc agggcgtgct gcccctccgt gacctggtga ccagccgggc cgaccgcccg
     1081 gtgcgggagt tcatggaggc cgtggtccac tcggtgcggc cggaggagga ccaggaggcg
     1141 gtggcgcggc tgatgtcgcg ctacaacctg ccgagcgtgc cggtgatcga ccaggagggg
     1201 cggctcctgg gccgcgtcac cttcgacgac gtgatcgacg tcgtcgaggc cgaaggcacc
     1261 gaggacatgc tcaagttcgg cggcaccagc gccgacgaag aggtggccgg cacctggtgg
     1321 caggcggtga agagccgcct cccctggctc tacgtcaacc tggccaccgc gttcctcgcg
     1381 ggcggcgtgg tcaagatctt cctcgatacc gtcaacaggc tgaccgacct cgccatctgg
     1441 atgcccatca tcgcggggat gggagggaac gccggcaccc aggcgctcgc cgtctcggtc
     1501 cgcggcctgg ccctcggcct cctcacgatg aaggaattct ggcgcgtcat cgggaaggag
     1561 atgctcgtcg gcctcgtcaa cggcgccgcc aacggagcgg tgagcgccct cgtcgccctg
     1621 ctgatccacg agaccccgat gctcggggtc tgcgtcttcc tgtccatgac ggggaatctc
     1681 ttcgtcgccg ggttcgccgg cgcgttcatc ccgatgatcc tcaagcggct gaacgtggac
     1741 cccgccatcg cctcgtcgat cttcgtgacg acgttcaccg acgtctgcgg gttcctgatg
     1801 ctgttcatga ccgcgcgggc gctgatcctg tgacgccggg cggagactga ggccgaggcg
     1861 cgcctccttg cccctccggc ccgcccacgc gacctttcct tatatgcgcg tgtccgccct
     1921 ggcctccgag atcacgtccg gcaacacccc ggcgctggcc cgggccatct cggtcgtgga
     1981 gaacgggcgt cccggcttcg aggagctgct ggtcgggctc catccccggc tcggccgggc
     2041 gcgacggatc gggatcaccg gccctccggg tgccggcaag tcgacgctca ccgagcggct
     2101 catcctggcc ctgcgcgccc aggggcacaa ggtcgcggtg gtggccgtcg atcccaccag
     2161 tcccttcacc ggcggcgccc tcctcggcga ccggatccgg atggagtcgg tggcgctcga
     2221 ccccgacgtc tacatccgct cgatggccac ccgcggatcg ctcggcgggc tcgccacctc
     2281 cacccgcgaa gtctgcgacg tgctcgacgc tgccgggttc gaccggatcc ttatcgagac
     2341 cgtcggggtg gggcagtcgg agctggccgt cacccgctcc gccgacacca cggtcctcgt
     2401 gctcgttccc gagtcgggcg acgggatcca ggcgctcaag tccggcgtca tggaagcggc
     2461 cgatgtgtac gtggtcaaca aggccgaccg ccccggcgcc gaccgcctgc gccaggagat
     2521 cgaggtctcc ctcggcatca ggtccggcca cggcttccgg cacgtcccgg cacaccacgg
     2581 cgccctcctg tcggcgcggc gagtgcgcgc cgaggcgggc gatcccgccg ccgacgcctg
     2641 gctgccgccg gtgcttgcca cggtcgcggc ggcgggggac ggggtggacg acctcgtcac
     2701 cgccctcgac cggcacgccg agtggctgga gcggagcggg gagctggatg agcgccggcg
     2761 gcggcggatc agcgaccgga tccgcgaagt ggtggcccgg cacctgcaac agtggctctg
     2821 ggatgagacc caggccgacc ggatggtgcg cgagcgcctc gatgaggtgg cggcggggcg
     2881 gcaaagcccg tacgaggtcg ccgccgaggt gctcgatcag gtgcgccagg gcgcacgcgt
     2941 ctagggcaag gagggggacc gatggccaag gggcatctgg aggcggagct ggcccggaag
     3001 gaccgggagc tcgagcggct gcgcgccgag gtcgatgcgt ggcggcgggt cgcgggcgcg
     3061 ctgcccgtgc gcgacgacag cgacttcacg accatctccg gccacgccgt ggagccgctc
     3121 tacaccgcgc tcgacctcgg tgatgccggg gcggcggcgg tggggctccc cggagaattc
     3181 ccctataccc gcggcatcca ccacaccggc taccgcggca agctctggac gatgcggcag
     3241 ttcgccggct tcggcaccgc cgaggacacc aaccgccgct acaagttcct gctcgagcgg
     3301 ggccagaccg gcctctcggt ggcgttcgac ttcccgaccc tgatgggcta tgactccgac
     3361 cacccccggt cggagggcga ggtcggcaag tgcggtgtcg ccatctcgag cctggccgac
     3421 atggagaccc tcttcgacgg catcccgctc gaccgggtgt cggtctcgat gacgatcaac
     3481 ggcccggccg cgatgctctt ctgcttcttc gtggccgccg ccgagaagca gggcatcccg
     3541 atcgagaagc tgcagggcac catccagaac gacattctca aggagtacat ggcgcagcac
     3601 gcgtggatct accctgcgga gccggcgctc aaggtcatcg tggacctctt cgagtggggg
     3661 agcgtccaca cccccaagtg gaacaccatc tccatctccg gctaccacat ccgcgaggcc
     3721 ggcgccaccg ccgcgcagga gctcgcattc acgctggccg acggcttctg ctacgtggag
     3781 catggcgtgg cgcggggcct cgacgtcgac gcgttcgcgc cccggctctc gttcttctgg
     3841 gacgtccaca acgacttctt cgaggagatc gccaagctcc gggccgcgcg ccgcatctgg
     3901 gcccgcgtcc tgcgcgacaa gtacggcgcg aaggacccgc gcagctggaa gatgcgcttc
     3961 cactgccaga ccgccggcgt ctcgctcacc gcccagcagc cgatgaacaa cgtcgtccgg
     4021 gtggcctacc aggcgctcgc cgcggtactg ggcggcaccc agtcgctcca caccaacgcg
     4081 ctcgacgaga cgctggcgct ccccaccgag cagtcggtgc gggtggcgct ccgcacccag
     4141 cagatcctcg cctacgagac cggcgtgccc aacacccacg acccgctcgg cggttcctgg
     4201 ttcgtcgagg cgctcaccga ccagatggag cgcgaggcga tgggcctctt cgcggagatc
     4261 gacgcacagg gcggcgtggt ggccgccatc gactccggct ggttccagcg ccagatcgcc
     4321 cgctccgccg cccgcttcca gtccgaggtc gaggccgggt ccaaggtggt cgtgggcttg
     4381 aaccgcttcg aggaggcgga ggaagcggca ccggagatcc tcaagatctc cgaccaggcc
     4441 gagcggcagc agcgggagcg gatggcgcgg atgcgcgccg gccgcgacca ggcgctcgtc
     4501 gaccgccggc tggaggcgct ccgctcggcg gcggtcgcgg accagaacgt gatcccggcg
     4561 atgctcgact gcgcgcgcgc gtattgcacg ctcttcgaga tccgccacgt cctcgagaac
     4621 gtctacggct cctaccggga gccggtcttc ttctagcagg ccccctatgc catcccccat
     4681 cgcccgccag atcgaacggc tcgctcgcga acggagcggg ccgccggtcg tctcctgcta
     4741 cctcaagctc gaaccgcgcg accgcgcgcg gggcaagtac ctcatcaagc tcaagaaccg
     4801 gctcaaggcc gccgagcagg cgctcgaacg ccgcgacttc tcccgcgtcg agcgcgaagg
     4861 cgcccgggcc gacctcgagc gcctgcggga atacctcggc tcccacgacc ggctcccccc
     4921 cacccagggg atcgcggtgt tcgtgagcgg agcccgcaag ctcttcgagg ccatcccgct
     4981 gccgtcggtc caccgatccc gcctgtccgt cgagcgcgtg ccgctggtgc gcgagctggc
     5041 cggcgtcgag gacgagatcg ggcgcctgtt ggtggtggtg gccgaccggg cgagcgcccg
     5101 gttcttccag gtgacggcct tcggcgccga gccggtcggg tccccggtcc tggccgacga
     5161 ttcggcgccg gcccggccgc gcccccgctc cggccgccgg gtgtcctcgc gcgtcggcga
     5221 gcacgcctac cacaatcgga tccgcagcgt gcagcagcgg cactgggagg cggtggccca
     5281 gcagctcttc ctgctcgacc ggaccgagcc ggcgcgggcc atcgtcatcc tggggcaggg
     5341 gccggcggcg gcggcgatcg ggccgttcct ccatccctac ctggccgagc gggtcatcgg
     5401 gcatgggacc ctcagcccgc gggaggccac gccgagcgcg gtgtatgccg ccgcgctcga
     5461 cgtgcgccat gagtgggagc gggcccgcga gcgggccgac gtcgccagga tgcgtgaacg
     5521 ggttggccgg gggtggtcca ccaacggcgt cgtgccgaca ctcaaggcgc tggccaccgg
     5581 gcaggtacgg acgctgctcg tgcacggcga tgccacggtg cccgggtacc ggctggccgg
     5641 gtccggccgc ctggtcatcc ccggcaagag cgaggcgtgg cgcggggagg gggagcccga
     5701 gcccgtggcc gatgtcatcg acgaggcgct cgaggaggcg ctccgccagc ggatcgacct
     5761 cgacgtgctc tatgacgccg aggccgcgcg cggtgtcgat ggcctcgcgg ccatcctgcg
     5821 gttcaagtga agcacccgcg ccgaaccacg aacttcttgc cgcctgaggt ccgacgatga
     5881 tgatgatggc tccgcagcag gcccccaccg ccacgcacaa gcgccccatc cgggtgctgg
     5941 tggccaagcc cggtctcgac ggccacgacc ggggcgcgaa agtggtggcg gcggcccttc
     6001 gtgacgcggg gatggaggtc atctacacgg gtctccacca gacgccggag atgatcgcgc
     6061 gcgccgcggt gcaggaggac gccgacgtcg tcgggttgtc gatcctgtcg ggcgcgcaca
     6121 tgacgctctt cccccgcgtc aagcagctgc tcgacgccga gggccggggt gacgtcctcc
     6181 tcaccggcgg cgggatcatc ccgcaggatg acatccagca gctcgaggcg atgggcgtcg
     6241 gccggctgtt cggccccggc acgccgacca cgtcgcttgt cgcctacatc caagagtggg
     6301 ccgccgccca cctccgcgat tgaccgccgc gccgcgcccc tcgcggctcc gcgagctcac
     6361 cgacgagtac caggcgctcg ccgtccgcct ctgccagggc ggcgggcccg agcgggccgc
     6421 caagatgcgg gccaaggggc agctcacccc gcgagaacgg gtg
//
LOCUS       DASUAP010000043         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1241045, whole genome shotgun sequence.
ACCESSION   DASUAP010000043 DASUAP010000000
VERSION     DASUAP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241045"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..2031
                     /gene="dxs"
                     /locus_tag="VFU45_01070"
     CDS             136..2031
                     /gene="dxs"
                     /locus_tag="VFU45_01070"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU5357679.1"
                     /translation="MTLLDGIKSPADLKRLKREQLPVLAQDIRDRMIDAVSVTGGHIG
                     AGLGVVELAIALLYEFDSPKDKVVWDVGHQAYPWKLLTGRNDRFDTLRQSGGISGFLK
                     RSESEHDQFGAGHAGTAMSAALGMATARDLKGEDYQVVAVVGDGALTCGLSYEGMNNA
                     GHSDRDILLIVNDNGMSISPNVGAISKMLGGIVASPAANRAREMVKKMTGSIPGPFGE
                     RVSEFARTVEESVKNLWSDGMLFEELGFRYFGPIDGHDLHKLGETLAFVRQMKGPRVL
                     HVLTQKGKGFPLAEANREKWHGLAAYDPVTGEARKKSSGPAPWTQAFGEALTAIAAQR
                     QDLVVITAAMPSGTGTNIFQKRWPERFFDVGIAEGHAVTFAAGLATQGLRPVVAVYST
                     FLQRAYDNIIHDVAVQRLPVIFAMDRAGLVGEDGQTHMGLYDIAYMLAVPEMTVTAPK
                     DGDELVGLLKCALQHDGPFATRYPRDKTPADPRPADEVPAVAYGTWELLREGKDLAIL
                     AVGTMVLPAVEAARKLAAEGIDCSVVNARFLKPYDRAMLESLVGRHRTLVTVEEGTVV
                     NGFGAYLAGILQTTRPEVRVVPLGVPDKLQEQAPRAEQLESFGLTADGIARRIVALRR
                     EESLEAR"
     gene            2028..2900
                     /locus_tag="VFU45_01075"
     CDS             2028..2900
                     /locus_tag="VFU45_01075"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEU5357680.1"
                     /translation="MNVGVVGNPRYGDLGPLIDVLAREAPKRGITLFSEPGLEGLFPG
                     PVGTLASAPLDFLITFGGDGTLLRGARLLDGREAPILGVNLGRVGFLTSAARDSLVEA
                     LDALATGHHAIDRRQALRASIVSADGTERSVTHALNDVVVHKGGVARVIRLRLHIGEE
                     EVGPYSADGLIVATPTGSTAYSLSAGGPVVVPGVEALVVTPICAHTLAVRPLVVHGGS
                     RVVIEPIAPWADDLLVSFDGQVGTTLVAGDRVHVRRADHRVLLVRLSTEDGFFSVMRR
                     KLHWGDLLGREPRP"
     gene            2897..>3220
                     /locus_tag="VFU45_01080"
     CDS             2897..>3220
                     /locus_tag="VFU45_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5357681.1"
                     /translation="MIAELRVRDLATIAEVTLQLGPGLNVLTGETGAGKSMLVDALAL
                     LLGERADSGLVRTSASRAVVEGAFEVADAPRRKRIEELGLDVDEGRVVVRREISAEGR
                     SRAWVN"
BASE COUNT          483 a         1192 c         1101 g          444 t
ORIGIN      
        1 ggatagcgga tagcggatag cggatagcgg atagcggata gcggatagcg gatagcggat
       61 agcggatagc ggatagtgga tagcggatag cggatagcgg atagcggatt accaaccccc
      121 ggagccccac agcgcatgac cctgctcgac ggcatcaagt ctcccgctga cctcaagcgc
      181 ctgaagcgcg aacagcttcc cgtgctcgcc caggacatcc gcgaccggat gatcgacgcc
      241 gtgtcggtga ccggcggcca catcggcgcg gggctcgggg tcgtggagct cgccatcgcg
      301 ctcctctacg agttcgactc gccgaaggac aaggtggtgt gggacgtggg gcaccaggcc
      361 tatccgtgga agctcctcac cggccgcaac gaccgattcg acaccctccg gcagtcgggt
      421 ggcatctcgg ggttcctcaa gcgcagcgag agcgagcacg accagttcgg ggccgggcac
      481 gccggcaccg cgatgtccgc cgcgctcggg atggccaccg cccgcgacct gaagggcgag
      541 gactaccagg tcgtggccgt cgtcggcgac ggggccctca cctgcgggct ctcctacgag
      601 gggatgaaca acgccggcca ctccgaccgc gacatcctcc tcatcgtcaa cgacaacggg
      661 atgagcatca gcccgaacgt cggtgccatc agcaagatgc tgggcggcat cgtcgcgagc
      721 cccgcggcca accgcgcccg ggagatggtc aagaagatga ccggctcgat cccggggccc
      781 ttcggcgagc gggtgtcgga attcgccagg acggtcgagg agagcgtcaa gaacctctgg
      841 tccgacggga tgctcttcga ggagctcggc ttccgctact tcggccccat cgacgggcac
      901 gacctccaca agctgggcga gaccctggcc ttcgtgcgcc agatgaaggg gccgcgggtc
      961 ctccacgtgc tgacccagaa ggggaagggg ttccccctgg cggaggccaa ccgggagaag
     1021 tggcacggcc tcgcggcgta cgaccccgtc accggcgagg cgcgcaagaa gtcgagcggg
     1081 ccggccccct ggacccaggc gttcggcgag gcgctcacgg cgatcgcggc ccagcgccag
     1141 gacctggtgg tcatcaccgc ggcgatgccc tcgggcaccg gcaccaacat cttccagaag
     1201 cggtggccgg aacggttctt cgacgtcggc atcgccgagg ggcacgcggt caccttcgcg
     1261 gcgggcctcg cgacgcaggg gctccgcccg gtggtggcgg tgtattccac cttcctccag
     1321 cgcgcctacg acaacatcat ccatgacgtg gcggtgcagc ggttgccggt gatcttcgcg
     1381 atggatcgcg ccgggctggt gggcgaggac ggccagaccc acatgggcct ctacgacatc
     1441 gcctacatgc tggcggtgcc ggagatgacg gtcaccgccc ccaaggacgg cgacgaactg
     1501 gtgggcctgc tcaagtgcgc gctccagcac gacggcccct ttgccacccg ctatccgcgc
     1561 gacaagacgc ccgccgaccc gcgccccgcc gacgaggtgc cggcggtggc gtacggcacc
     1621 tgggaactgc tgcgggaagg gaaggacctc gccatcctgg ccgtcggcac gatggtgctc
     1681 ccggcggtcg aagcggcgcg gaagctcgcc gccgagggca tcgactgcag cgtcgtcaac
     1741 gcacgcttcc tcaagcccta cgaccgcgcc atgctcgagt cgctggtcgg ccgccaccgc
     1801 acgctggtca cggtcgagga aggcaccgtg gtcaacggct tcggcgccta cctggccggg
     1861 atcctccaga ccacccggcc cgaggtgcgc gtcgtgccgc tcggcgtccc cgacaagctc
     1921 caggagcagg cgccgcgtgc ggagcagctc gagtcgttcg ggctcaccgc cgatggcatc
     1981 gcccgccgca tcgtggcgct ccgccgcgag gagagcctcg aggcccgatg aacgtcggcg
     2041 tcgtcggcaa cccgcgctac ggcgacctgg gcccgctcat cgacgtcctc gcccgcgagg
     2101 cgccgaagcg ggggatcacg ctcttctctg agcccggcct cgaggggctc ttccccgggc
     2161 ccgtcggcac gctcgcgtcg gccccgctcg atttcctcat caccttcggc ggcgacggca
     2221 cgctgctccg cggcgcccgg ctcctcgacg gccgcgaggc cccgatcctg ggcgtcaacc
     2281 tcggccgcgt cgggttcctc acctcggccg cgcgcgactc gctggtcgag gcgctcgacg
     2341 ccctcgccac cgggcaccac gccatcgacc gccgccaggc gctccgcgcc tcgatcgtct
     2401 cggccgacgg caccgaacgc tccgtcaccc acgccctcaa cgacgtcgtg gtccacaagg
     2461 gcggcgtcgc ccgtgtcatc cgcctccggc tccacatcgg cgaggaggaa gtgggcccct
     2521 acagcgccga cggcctgatc gtggccacgc ccaccggctc caccgcctac tcgctctcgg
     2581 ccggcggtcc ggtcgtggtc cccggcgtcg aggcgctggt ggtgaccccg atctgcgccc
     2641 acaccctcgc cgtccgcccc ctcgtggtgc acggggggag ccgcgtggtg atcgaaccga
     2701 tcgcgccctg ggccgacgat ctcctcgtct ccttcgacgg ccaggtgggc accacgctcg
     2761 tcgccggtga ccgggtgcac gtccgccgcg ccgaccaccg ggtgctcctg gtccggctct
     2821 ccaccgagga cggcttcttc agcgtcatgc gccgcaagct ccactggggc gacctcctcg
     2881 gccgcgagcc gcggccgtga tcgccgagct ccgcgtccgc gacctcgcca cgatcgccga
     2941 ggtcaccctc cagctcggac ccggcctcaa cgtcctcacc ggcgagaccg gcgccggcaa
     3001 gtcgatgctc gtcgacgcgc tggccctcct cctcggcgaa cgcgccgaca gcggcctggt
     3061 ccgcaccagc gcatcgcgcg cggtggtgga aggcgccttc gaggtggccg acgccccgcg
     3121 ccggaagcgg atcgaggagc tcggcctcga cgtcgacgag ggacgcgtgg tggtccggcg
     3181 cgagatctct gcggaaggac gctcccgcgc gtgggtcaac
//
LOCUS       DASUAP010000044         4886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_573514, whole genome shotgun sequence.
ACCESSION   DASUAP010000044 DASUAP010000000
VERSION     DASUAP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573514"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="VFU45_01085"
     CDS             complement(<1..1533)
                     /locus_tag="VFU45_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5357682.1"
                     /translation="MTGPADRLAAALADRYRIERELGAGGMATVYLAQDVRHDRKVAV
                     KVLRPELAAVIGAERFVVEIKTTAQLQHPHILPLFDSGEAEGFLYYVMPYVEGETLRD
                     TLNRETQLGIDEAVRITVAVADALDYAHRHGVIHRDIKPENILLHDGRPMVADFGIAL
                     ALSAAAGGRMTETGMSLGTPHYMSPEQATADKEITGRSDIYSLASMLYEMLTGNPPHT
                     GASAQQIIMKIIAEPAQPVTAYRKSVPPNVAAAVARALEKLPADRFQTAKAFAEALGD
                     PGFRATGADATVGASARRSRGAVAALGGAVVVLAALALWGWLRPAPLHPVRRYALGMA
                     AGQAIRSGVIGVNIAVSPDGSKLVYVGADTGQGTQLWLRDRDRLDATPIPGTAGATNP
                     FFSPTGDRIAFSIGVQYDLRVVSVAGGPATTLATMGPQSAGGGTWSAAGYIYEDSPGG
                     LSRVPAAGGTPHLAVPLDSAAGEIGYAWPAALPNGRGLLFRRRLSTDVADFDVMAVDL
                     RTG"
     gene            1746..>4886
                     /locus_tag="VFU45_01090"
     CDS             1746..>4886
                     /locus_tag="VFU45_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357683.1"
                     /translation="MSRSLRVVAVALLLASASASAQSPATYGEMHWRQIGPPRAGRAR
                     ALAGVPSQPNVFYAGFDNGGVWRTTDYGSTWQPLFDQEPTGSIGAIAVAPSDPNVIYV
                     GTGAGIIRPDLATGDGVYKSTDGGRTWAHLGLDGTQMIAWIAVDPANANRLFVAALGH
                     PYGPNPERGIFRSTDGGTTWQKVLYTDEYTSGNCVVIDPSDPNTVYAGLWQQQQSFRE
                     GDEFGGAGHGIYKSTDGGTTWTHLTAGLPDAIEANIAVAPSDPSRLYAMVAGVASAGA
                     RDKGGPTTGVVGLYTSTDRGEHWSAVDAPGVPADTSSPADPRPLSRIGGGDLPPVTVD
                     PKDPNVVYSSSVVLWRTMDGGHTWAAVRGAPGGDDYQNTWVNPNDPDILFVVSDQGAV
                     VSANRGKSWSNWYTQPTAAMYHATTDNAFPYRVCSGQQDSGSGCVDSRSMDGEITFHD
                     WHPVNIQEYGMAAPDPRDPDLVYGSGRTGISLYDRRTGQTTHIGPDQEERGADYGRNV
                     RTMPLIWSPVAPDVLFYASNVVWKSADHGHSWRRISPDLARATWAVPASAGKYADSVR
                     AKPEGAITALAPSPRDVKVLWAGTDDGSVQVTTDGGATWRNVTPAGIAPWTRIYNMDA
                     GHFSTGTAYVAANTLRIDDLNPHFWRTHDGGRTWTEIDHGIAPGSPVNSIREDPRVPG
                     LLYGATDTQVWVSFDDGDHWESLRLDMPAVSVRDLTVKDDKGCLCSDLVVATHGRGFW
                     ILDDVTPLRQAAAARAAREAYLFKPAPAVRVRFGTNDPTPWPPELPAGENPPPGGIID
                     YYLAADVHGPVGLEILDAAGAVVRSYSSDDSVLVPDPGREPAKYEDEVCEHKPDAADC
                     DVPLYWPAPQMRLSTAAGMHRFSWDLRYDPIDDRGPGSANGAAVPYHTYPSRYAPWAP
                     AGAYTVQLTVNGKRYTQPLRLYLDPRVRTPAPALAELAALTHEMYLGARAAHQDAVRA
                     HALAAVLAKADGTDVAALRAELDSIAPAPKPEGGRGFRRRRNGAKAAPTLGAVSGEML
                     GAAMAMQEADVAPTAEN"
BASE COUNT          703 a         1780 c         1747 g          656 t
ORIGIN      
        1 cccggtgcgg aggtcgacgg ccatcacgtc gaagtcggcc acgtcggtgc tcaggcgccg
       61 ccggaagagc agcccgcgtc cgttggggag cgccgcgggc caggcgtagc cgatctcgcc
      121 ggccgcggaa tcgaggggca cggcgaggtg aggggtgccg ccggccgctg gcacccggct
      181 cagtccgccg ggggagtcct cgtaaatgta tccggcggcg ctccacgtgc cgcccccggc
      241 gctctgcggg cccatcgtgg cgagcgtcgt ggcgggcccc ccggcgaccg acacgacccg
      301 aaggtcgtac tgtacgccga tggagaaggc gatccggtcg ccggtcggcg agaagaacgg
      361 attggtggcc cctgccgtcc cggggatcgg agtcgcatcg agccggtcgc ggtcgcggag
      421 ccagagttgc gttccctgcc cggtgtcggc gccgacgtac accagcttcg agccgtcggg
      481 ggagaccgcg atgttgaccc cgatcacccc cgagcggatc gcctgtccgg cggccatccc
      541 gagcgcgtag cggcggaccg ggtggagcgg cgcgggccgg agccagcccc acagcgcgag
      601 cgccgccagc accacgacgg cgccgccgag cgcggccacc gcgccccggg agcgccgcgc
      661 ggacgcgccg accgtggcat cggctccggt cgcgcggaag ccaggatcgc cgagcgcctc
      721 ggcgaacgcc ttcgcggtct ggaaccggtc agcggggagc ttctcgaggg cgcgggccac
      781 cgcggcggcg acgttgggcg gcaccgactt ccggtacgcc gtgaccggct gcgccggctc
      841 ggcgatgatc ttcatgatga tctgctgcgc gctcgccccg gtgtgcggcg ggttcccggt
      901 cagcatctcg tagagcatgc tcgccaggga gtagatgtcg ctccgcccgg tgatctcctt
      961 gtcggcggtg gcctgctcgg ggctcatgta gtgcggcgtg ccgagcgaca tccccgtctc
     1021 ggtcatccgg ccgccggccg ccgccgagag ggcgagcgcg atcccgaagt cggccaccat
     1081 cgggcggccg tcgtggagga ggatgttctc tggcttgatg tcgcggtgga tgacaccgtg
     1141 gcggtgggcg tagtcgagcg catcggccac ggccacggtg atccgcaccg cctcgtcgat
     1201 cccgagctgg gtctcgcggt tcagcgtgtc gcggagggtc tccccttcga cgtagggcat
     1261 cacgtagtag aggaagccct cggcttcccc cgagtcgaac agcggcagga tatgcggatg
     1321 ctggagctga gcggtggtct tgatctcgac cacgaaccgc tccgcgccga tgacggcggc
     1381 gagctcgggc cgcaggacct tgaccgcgac cttgcggtcg tgccggacgt cctgggccag
     1441 gtacaccgtg gccatcccgc cggcgccgag ctcgcgttcg atgcgatagc ggtcggcgag
     1501 ggccgcggcg aggcgatcgg cgggcccggt catcgggcgc ccgcgtccgt cgggtacacg
     1561 tgaagcactc tggaccgcgc ttgtatggaa gggaacgccc gggcacggat gccccaggac
     1621 gctggtgaag tatatcattt ccgtggcagg ctcgccccac gcgccgtcgg gcgtggcaga
     1681 atgggccccg tcacgccata ctccgtgcac ctccgaaccg tgctttcctt taataggcgg
     1741 tccccgtgtc ccgttccctc cgtgtcgtcg ccgtcgccct gctcctcgcc tctgcctccg
     1801 cctccgccca atcgcccgcc acctacggcg agatgcactg gcggcagatc ggtcccccgc
     1861 gcgccggccg cgcccgcgcc ctggccgggg tgccgagcca gccgaacgtc ttctatgcgg
     1921 ggttcgacaa cggcggcgtc tggcggacca ctgactacgg ctccacctgg cagccgctct
     1981 tcgaccagga gcccaccggc tcgatcgggg ccatcgcggt cgcgccgtcg gatccgaacg
     2041 tcatctacgt cggcaccggc gccgggatca tccgtcccga cctggccacc ggcgacggcg
     2101 tctacaagtc caccgacggc ggcaggacct gggcccacct gggcctcgac gggacgcaga
     2161 tgatcgcgtg gatcgcggtg gaccccgcca acgccaaccg tctcttcgtc gccgcgctgg
     2221 gccacccgta cggccccaac ccggagcggg ggatcttccg ctccaccgac ggcggcacga
     2281 cctggcagaa ggtgctgtac accgacgagt acacgagcgg gaattgcgtc gtcatcgacc
     2341 cgtccgatcc caacaccgtc tacgccgggc tctggcagca gcagcagagc ttccgcgagg
     2401 gcgatgagtt cggcggcgcg ggacacggca tctacaagtc gaccgacggc ggcaccacct
     2461 ggacccacct gaccgccggc ctccccgatg cgatcgaggc gaacatcgcg gtcgcgccga
     2521 gcgacccgag ccggctctac gcgatggtgg cgggcgtggc gtcggcgggt gccagggaca
     2581 agggcggccc gaccaccggc gtggtcggtc tctacacctc caccgaccgg ggcgagcact
     2641 ggtccgcggt cgacgcgccc ggcgtgccgg ccgacacttc cagtccggcc gatcctcggc
     2701 ccctctcgcg gatcggcggc ggtgacctgc cgcccgtcac cgtggatccg aaggatccga
     2761 acgtcgtgta cagctccagc gtcgtgctct ggcggacgat ggacggcggg catacctggg
     2821 ccgcggtgcg cggtgccccc ggcggcgacg attaccagaa cacctgggtc aaccccaacg
     2881 atccggacat cctcttcgtg gtgtcggacc agggggccgt ggtctccgcc aaccgcggga
     2941 agtcgtggag caactggtac acccagccga ccgcggcgat gtaccacgcc accaccgaca
     3001 acgcgttccc gtatcgcgtc tgcagcggac agcaggactc cggctcgggg tgtgtcgaca
     3061 gcaggtcgat ggacggcgag atcaccttcc acgactggca tccggtcaac atccaggagt
     3121 acgggatggc ggcgcccgat ccccgcgacc ccgacctggt gtacgggagc ggccggaccg
     3181 gtatctcgct ctacgaccgc cggaccggcc agacgaccca catcggcccc gaccaggaag
     3241 agcgcggcgc cgactacggc cgcaacgtgc gcacgatgcc gctcatctgg tcgccggtgg
     3301 cgccggacgt gctcttctac gcctcgaacg tggtgtggaa gagcgccgac cacgggcaca
     3361 gctggcgccg gatcagcccc gacctggcgc gggcgacgtg ggccgttccc gcgagcgcgg
     3421 ggaagtacgc cgacagcgtc cgcgcaaagc ccgaaggcgc catcaccgcg ctcgcgccct
     3481 cgccgcgtga cgtgaaggtg ctctgggcgg ggaccgacga cggctccgtc caggtgacca
     3541 ccgatggcgg cgccacctgg cgcaacgtga cgcccgcggg gatcgcgccg tggacccgga
     3601 tctacaacat ggatgccggg cacttcagca ccggcaccgc ctacgtcgcc gccaatacgc
     3661 tgcgcatcga cgacctgaac ccccacttct ggcgcacgca cgacggcggg cgcacctgga
     3721 ccgagatcga ccacgggatc gcgcccgggt cgccggtcaa ctcgatccgc gaggatccgc
     3781 gggtgccggg gctcctctac ggcgccaccg acacgcaggt ctgggtctcc ttcgacgatg
     3841 gcgaccactg ggagtcactg cggctcgaca tgccggcggt gtcggtgcgg gacctcaccg
     3901 tcaaggacga caaggggtgt ctctgctccg atctcgtggt cgccacccac ggccgaggct
     3961 tctggatcct cgacgacgtg acgccgctcc ggcaggccgc ggcggcgcgg gcggccaggg
     4021 aggcgtacct cttcaagccg gcgcccgcgg tgcgggtccg cttcggcacc aacgacccga
     4081 cgccgtggcc gccggagctc ccggcggggg agaacccgcc gccgggcggg atcatcgact
     4141 attacctcgc cgccgacgtg cacggcccgg tggggctcga gatcctcgac gccgcgggcg
     4201 cggtggtacg cagctattcg agcgacgact cggtgctcgt ccccgatccg ggccgcgagc
     4261 cggcgaagta cgaggacgag gtctgcgagc acaagcccga cgccgccgac tgcgacgtgc
     4321 cgctctactg gccggcgccg cagatgcggc tctccacggc ggcggggatg caccggttca
     4381 gctgggatct ccgctacgac ccgatcgacg accgcgggcc cggctcggcc aacggcgcgg
     4441 cggtgccgta ccacacctat ccctcgcgct acgccccgtg ggcgcccgcc ggcgcgtaca
     4501 cggtccagct cacggtgaat ggcaagcggt acacccagcc gctccggctc tacctcgacc
     4561 cgcgggtcag gacgccggcg ccggcgctgg cggagctcgc ggcgctgacc cacgagatgt
     4621 acctgggcgc cagggcggcc caccaggacg ccgtccgcgc ccacgcgctc gcggcggtgc
     4681 tcgccaaggc ggatggcacg gacgtggcgg cgctccgggc ggagctcgat tcgatcgcgc
     4741 cggcgccgaa gccggaaggc gggcgcggct tccgccgccg ccggaacggg gccaaggccg
     4801 cgcccacgct cggcgcggtg agcggcgaga tgctgggtgc cgcgatggcg atgcaggagg
     4861 cggacgtggc gccgacggcg gagaac
//
LOCUS       DASUAP010000045         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_55598, whole genome shotgun sequence.
ACCESSION   DASUAP010000045 DASUAP010000000
VERSION     DASUAP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55598"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFU45_01095"
     CDS             <1..876
                     /locus_tag="VFU45_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU5357684.1"
                     /translation="KVHGGKMRAWRFPEIVICVPSGSTSVERRASRDAASNAGASAVY
                     LIEEPMAAAIGADMPVTAPIGSMVVDIGGGTTEVAVLSLRGLAYTTSVRVGGDKMDEA
                     ISSYVRRNHNLLIGEATAERIKKEVGIAKPPVDGIGKTVHIKGRDLVNGVPKEISINQ
                     GQIAEALSEPVGTIVEGVRIALENTAPELAADICDFYDALRTNRPYRGAWESDKVLAY
                     IEAGLGREFDADAGHAFVEMMREWGQRSVVVDESTPVRSDVRISGAQHARTSGGQPAV
                     PAAEAPATPPGPAAQ"
     regulatory      1018..1124
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1189..>2269
                     /locus_tag="VFU45_01100"
     CDS             1189..>2269
                     /locus_tag="VFU45_01100"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEU5357685.1"
                     /translation="MATTADRPAARPAGSSFEEAFPGSRKIHVEGPHGIQVPFREISL
                     SGGEPALRVYDTSGPQGNDVRLGLPELRAPWIRARGVTETGAISGGKEELMPPTLRRK
                     VLRGAGRVTQMHYARKGEVTPEMEFVALREGLPADFVRAEVARGRAIIPANINHPELE
                     PMAIGRNFKVKINANIGNSAVTSSIEEEVEKLRWSTLWGADTVMDLSTGRNIHETRGW
                     ILRNSAVPIGTVPIYQALEKVGGVAEDLTWEAYRDTLIEQAEQGVDYFTVHAGVLLRF
                     IPLTADRVTGIVSRGGSIMAKWCLAHHQESFLYTHFREICEILAAYDVSFSLGDGLRP
                     GSIADANDEAQFAELRVQGELNRIAW"
BASE COUNT          375 a          771 c          796 g          327 t
ORIGIN      
        1 aaggtgcatg gcggcaagat gcgcgcctgg cgctttccgg aaatcgtgat ctgcgttccg
       61 tcggggtcga cctccgtcga acgccgcgcg agccgcgatg cagcgtccaa tgcaggcgcg
      121 agcgcagtct atctgatcga ggagccgatg gccgcagcga tcggcgccga catgccggtg
      181 accgcgccga tcggctcgat ggtggtcgac atcggcggcg gaacgaccga ggtcgccgtc
      241 ctctcgcttc gcggcctcgc ctacaccacc tcggtccgcg tcggcggcga caagatggac
      301 gaagcgatca gctcctacgt ccgccgcaac cataaccttc tcatcggtga agccacggcc
      361 gagcggatca agaaggaagt cgggatcgcc aagccgccgg tcgacggcat cggcaagacc
      421 gtccacatca agggccgcga cctcgtaaac ggcgttccca aggaaatctc gatcaaccag
      481 ggccaaatcg ccgaggcgct gtccgaaccg gtcgggacga tcgtcgaggg cgtcagaatc
      541 gcgctcgaga acaccgcgcc ggagctcgcc gccgacatct gcgacttcta cgacgcgctc
      601 cggaccaacc gcccgtaccg tggggcgtgg gagtcggaca aggtgctggc ctacatcgag
      661 gcgggactcg gccgcgaatt cgacgccgac gccggccacg ccttcgtcga gatgatgcgg
      721 gagtggggcc agcggagcgt cgtcgtcgac gagtcgaccc cggtccggag cgacgtgcgc
      781 atctccgggg cgcagcacgc gaggacatcg ggtgggcagc cggcggtgcc ggcggcggaa
      841 gcgccggcca cgccacccgg gccggccgct cagtagctgc agaccgcgcc ctccggcccc
      901 ctctccctta cgggagaggg ggaagtcgtt caatgcactc cccctctccc gtaagggaga
      961 gggggtcggg gggtgaggtc ggaggctggc ctccgccgat ccccgccgat actattgact
     1021 gaccgccagg ggcatcccgc cgcgccagcg cgccgggatt gagatgagac ccttggaacc
     1081 tgatccggtt gataccggcg aagggagtgc ggtgtggatg ccgccttcct gcgacctcgc
     1141 cggagcggca tttcgtgttt ccgggccccg tgccctgagg agagtctgat ggccaccacc
     1201 gccgaccgtc ccgccgcccg acccgccggc tcctcgttcg aggaggcgtt ccccgggtcg
     1261 cggaagatcc acgtcgaagg cccccatggc atccaggtcc cgttccggga gatctccctc
     1321 tccggcgggg agccggcgct ccgggtgtac gacaccagcg ggccgcaggg caacgatgtc
     1381 cggctgggac ttccggagct ccgcgccccc tggatccgcg cccgcggcgt gaccgagacc
     1441 ggcgccatct ccggtggcaa ggaggaactg atgccgccga cgctccggcg gaaggtgctc
     1501 cgtggggcgg ggcgcgtcac ccagatgcac tacgcccgca agggcgaggt gacgcccgag
     1561 atggagttcg tggcgctgcg cgaagggctg ccggccgact tcgtgcgcgc ggaggtggcg
     1621 cgggggcggg ccatcatccc cgccaacatc aaccaccccg aacttgagcc gatggccatc
     1681 ggccggaact tcaaggtcaa gatcaacgcc aacatcggca actcggccgt gacgtcgtcg
     1741 atcgaggagg aggtggagaa gcttcgctgg tccaccctct ggggtgccga cacggtgatg
     1801 gacctttcga cgggaaggaa catccacgag acccgcgggt ggatcctccg caactccgcc
     1861 gtgccgatcg gcacggtgcc gatctaccag gcgctcgaga aggtgggcgg cgtggcggag
     1921 gacctgacgt gggaggcgta tcgcgacacg ctgatcgagc aggcggaaca gggggtcgac
     1981 tacttcacgg tgcacgccgg cgtgctgctg cggttcatcc cgctcaccgc cgaccgggtc
     2041 accgggatcg tgtcccgcgg cgggtcgatc atggccaagt ggtgcctggc ccaccaccag
     2101 gagagctttc tctacaccca cttccgcgag atctgcgaga tcctggcggc gtacgacgtc
     2161 tcgttctccc tgggcgacgg gctccgtccc gggtcgatcg ccgacgccaa cgacgaggcg
     2221 cagttcgccg agctccgggt gcagggggag ctcaaccgga tcgcgtggg
//
LOCUS       DASUAP010000046         3594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1050494, whole genome shotgun sequence.
ACCESSION   DASUAP010000046 DASUAP010000000
VERSION     DASUAP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050494"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFU45_01105"
     CDS             <1..594
                     /locus_tag="VFU45_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5357686.1"
                     /translation="TAADREARVRELLGKVGLRPDVAARYPHELSGGQRQRVGIARAL
                     AVEPRFIVLDEPVSALDVSVQAQVVNLLADLQQRRGLSYLFIAHDLAVVRQIARRVAV
                     MYLGRLVEVGPVEEVLSRPKHPYTQALLAAVPEPDPAVRPVRLVIPGEPPSPSAPPPG
                     CPFHPRCQHPGVDARCRTERPDLRELGADRVACHHAV"
     gene            658..1053
                     /locus_tag="VFU45_01110"
     CDS             658..1053
                     /locus_tag="VFU45_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5357687.1"
                     /translation="MSRQLIASGAPWEAIVGYSRAVRVGNVVHVAGTTATGPDGAIVG
                     ARDAYRQMQQALRNIEAALATAGATLGDVVRTRVFVTDISRWQEVGKAHGEAFADIRP
                     ASTMVQVAALVDPAMLVEVEVDAIIGSAG"
     gene            1090..1878
                     /locus_tag="VFU45_01115"
     CDS             1090..1878
                     /locus_tag="VFU45_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEU5357688.1"
                     /translation="MPTPLIQVDAFTDKPYHGNPAAVCLLSAPMDDAWLRNVANEMNL
                     SETAFLLPEKDQWRLRWFTPTVEVDLCGHATLASAHVLWELGKLKRDQPARFITRSGL
                     LTATAADGWIELDLPADAAEETAAPAELVEALHTPIVHTAQGKFDYLVEVESEALVRE
                     LAPDIRLLGELTTRGICVTAPATTKGYDFVSRFFAPGAGIAEDPVTGSAHCMLGPWWA
                     RRLGRDTMTAAQVSARGGVVRVQVGPERVRLGGQAVTVLRGELA"
     gene            1875..3014
                     /locus_tag="VFU45_01120"
     CDS             1875..3014
                     /locus_tag="VFU45_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5357689.1"
                     /translation="MSDPAGARHFETLAAHAALGPDPATGAAVPPIVLSTTFERAPDG
                     TFPHGYIYSRTANPNRTALEAALAGLEGGAACAAFASGQAAVLAVFQALAPGDHIVAP
                     ADVYHGVRTLLKDIFERWGLAVTFADLSDLGAAERAITAKTKLVWAESPSNPLLRITD
                     LAKLAELAHARGALLAVDNTWATPALQRPLALGADLVMHSTTKYLGGHSDVLGGAIVA
                     RGADGFFAGIRKVQQVGGAVPSPFDAWLVARGIRSLPSRMRTHCENAARVAAFLAARK
                     GVAAVHYPGLATHPGHDVARRQMSAFGGMLSIEVEGDAARAQRVAARVKVFLRATSLG
                     GTESLIEHRRSVEGPYSTTPETLLRLSIGLEHPDDLIADLDQAIG"
     gene            3055..3384
                     /locus_tag="VFU45_01125"
     CDS             3055..3384
                     /locus_tag="VFU45_01125"
                     /inference="COORDINATES: protein motif:HMM:NF007495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEU5357690.1"
                     /translation="MTPTEAFETLDVRVGRIVEATVNARARKPSYVLRIDFGPLGHRT
                     SSAQLTVHYTPEQLIGRQVVAAMNLGTRNIAGVVSEVLVLGLPDQDGAVVLLAPEREV
                     PLGGRMY"
     gene            3425..>3594
                     /locus_tag="VFU45_01130"
     CDS             3425..>3594
                     /locus_tag="VFU45_01130"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEU5357691.1"
                     /translation="MAESVYKVIELVGTSSVSWEKAAAAAVARASKSLRDLRVAEVTQ
                     LDMAIKNGKVES"
BASE COUNT          504 a         1354 c         1243 g          493 t
ORIGIN      
        1 accgcggccg accgcgaggc gcgggtgcgc gagctgctcg gcaaggtcgg gctccggccc
       61 gacgtcgcgg cgcgctaccc gcacgagctg tcgggcggcc agcgccagcg cgtcgggatc
      121 gcgcgggcgc tcgcggtcga acctcgcttc atcgtgctcg acgagccggt ctccgcgctc
      181 gacgtgtcgg tccaggcgca ggtcgtcaac ctcctggccg acctgcagca gcgccgcgga
      241 ctctcctatc tgttcatcgc ccacgacctc gccgtcgtgc ggcagatcgc gcggcgcgtg
      301 gcggtgatgt acctggggcg gctggtcgag gtgggcccgg tcgaggaggt gttgtcccgg
      361 ccgaagcacc cctacaccca ggcgctcctg gccgcggtgc cggaacccga ccccgcggtg
      421 cggcccgtcc gcctcgtcat cccgggcgag ccaccgagcc cctcggcgcc acctcccgga
      481 tgccccttcc atccccgctg ccagcacccc ggcgtcgacg cccggtgccg caccgaacgc
      541 cccgacctcc gcgagctcgg cgccgaccgc gtcgcgtgcc atcacgcggt ctgaatcggc
      601 tcgcggcgcg gcagtcacgc gcctaaattc cggcgtcccg tccaccccgg agcgcagatg
      661 tcccggcaac tgatcgcgag cggcgcgccg tgggaggcca tcgtcggcta ttcccgcgct
      721 gtgcgcgtcg gcaatgtcgt gcacgtggcg ggcaccaccg ccaccggccc cgatggcgcc
      781 atcgtcggcg cccgcgacgc ctaccggcag atgcagcagg cgctccggaa catcgaggca
      841 gcgctggcca cggccggcgc cacactgggc gacgtcgtcc gcacccgggt cttcgtgacc
      901 gacatctccc ggtggcaaga ggtcggcaag gcgcacggtg aggcgttcgc cgacatccgg
      961 ccggcatcaa cgatggtcca ggtcgcggcg ctggtggatc ccgcgatgct cgtcgaggtc
     1021 gaggtcgacg cgatcatcgg ttcggccggc tgacgcatcc cgtcgccgga acccttcctt
     1081 ccagagacca tgcccactcc gctcatccag gtcgacgcct ttaccgacaa accgtaccac
     1141 ggcaaccccg ccgccgtctg cctcctgtcg gcgccgatgg acgatgcgtg gctccggaac
     1201 gtcgcgaacg agatgaacct ctcggagacg gcgttcctgc tccccgagaa ggaccagtgg
     1261 cggctccgct ggttcacccc gacggtcgag gtcgacctct gcggccacgc gaccctggcc
     1321 tccgcccacg tgctctggga actgggcaag ctcaagcgcg accagcccgc ccgcttcatt
     1381 acccggagcg gcctcctcac ggccaccgcg gccgacggat ggatcgagct cgacctcccg
     1441 gccgacgcgg ccgaggagac cgccgccccg gcggagctgg tggaagcgct gcacacgccc
     1501 atcgtgcaca cggcgcaggg gaagttcgac tacctggtcg aggtcgagtc ggaggcgctg
     1561 gtgcgcgaac tcgcccccga catccgcctc ctcggcgaat tgaccacccg cggcatctgc
     1621 gtcaccgcgc ccgccacgac caagggctat gacttcgtct cccggttctt cgcgccgggc
     1681 gccgggatcg ccgaggaccc ggtgaccggg tcggcgcact gcatgctcgg gccctggtgg
     1741 gccaggcgcc tcggccgcga cacgatgacc gcggcgcagg tctccgcccg cggcggcgtc
     1801 gtgcgggtgc aggtcggccc ggagcgggtg cggctgggcg gccaggccgt caccgtgctg
     1861 cggggagagc tcgcatgagc gacccggccg gcgcccgcca tttcgagacc ctcgcagccc
     1921 acgccgcgct cgggcccgat cccgccaccg gcgccgccgt gccgcccatc gtcctgtcca
     1981 ccaccttcga gcgcgcgccc gacggcacct tcccgcacgg ctacatctac agccgcaccg
     2041 ccaacccgaa ccggacggca ctggaagcgg cgctcgccgg gctcgaaggg ggcgccgcct
     2101 gcgccgcgtt cgcctcgggc caggcggcgg tgctggcggt gttccaggcg ctggcgccgg
     2161 gcgaccacat cgtggcgccg gccgacgtgt atcacggcgt ccgcacgctg ctcaaggaca
     2221 tcttcgagcg gtgggggctc gccgtcacct ttgccgacct gagcgacctc ggggcggccg
     2281 agcgggcgat cacggcgaag acgaagctcg tgtgggccga atcgccatcg aacccgctgc
     2341 tgcggatcac cgacctggcg aagctcgcgg agctggccca cgccaggggc gcactcctcg
     2401 cggtggacaa cacctgggcc acaccggcgc tgcagcgccc cctggccctg ggcgccgacc
     2461 tggtgatgca ctccaccacc aagtacctgg gcggccacag cgacgtgctg ggcggcgcca
     2521 tcgtcgcccg gggcgccgat ggattctttg cggggatccg gaaggtgcag caggtcggcg
     2581 gggcggtccc ctcgccgttc gacgcgtggc tcgtggcgcg gggcatccgg agcctcccga
     2641 gccggatgcg cacccattgc gagaacgccg cgcgggtggc cgcattcctg gcggcacgga
     2701 agggcgtcgc cgcggtgcac tatccggggc tcgccaccca tccgggccac gacgtggccc
     2761 ggcgccagat gagcgccttc ggcgggatgc tgtccatcga ggtcgaggga gatgcggcgc
     2821 gagcgcagcg cgtagcggcc cgggtcaagg tgttcctccg cgccacctcg ctcggcggca
     2881 ccgagagcct gatcgagcac cggcgctcgg tcgaggggcc gtattccacc acgcccgaga
     2941 ccctgctccg cctgtcgatc ggcctcgagc acccggacga cctgatcgcc gacctcgatc
     3001 aggccatcgg gtgaccgacg ccgcgactcg cgacctgcga accgtgacgc cttcgtgacc
     3061 cccaccgaag ccttcgagac gctcgatgtc cgtgtcgggc ggatcgtcga agccacggtc
     3121 aacgccaggg cccggaagcc gagctatgtc ctccggatcg acttcggccc gctcggtcac
     3181 cggacgtcga gcgcgcagct gaccgtgcac tacacccccg aacagctgat cggccgtcag
     3241 gtggtcgcgg ccatgaacct cggcacccgc aacatcgccg gcgtcgtatc cgaggtgctg
     3301 gtcctcgggc tccccgacca ggacggtgcg gttgtgcttt tggcccctga gcgggaagtt
     3361 cccctgggcg gccggatgta ctgaccggcg cacggcggcc gccatcctcc accaggagct
     3421 ccgtatggcg gaaagcgtct acaaggtcat cgaactggtc ggcaccagca gtgtgtcgtg
     3481 ggaaaaggcg gccgcggccg cggtcgcgcg ggcgtcgaag tcgctcaggg acctgcgggt
     3541 ggcggaagtg acgcagctcg acatggccat caagaacggg aaggtcgaga gcta
//
LOCUS       DASUAP010000047         7829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_668989, whole genome shotgun sequence.
ACCESSION   DASUAP010000047 DASUAP010000000
VERSION     DASUAP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668989"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFU45_01135"
     CDS             complement(<1..565)
                     /locus_tag="VFU45_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357692.1"
                     /translation="MTIRRSAILAASIAGLAVLVVARWARTGAPVGVYIDDGFYAILG
                     RALATGHGLSYIGWPGAPAPSHYPPGYPLLLAMLWRLGGSVDAVARWGEWINAGALAI
                     AAALVAAALRLRFGIAPWIAAPAAVLGAVATPVLAVSTLLLSEACFLLLLVLVLWMAD
                     RSGATAGRLGWAVAAGALAGTAALVRTI"
     gene            complement(615..1001)
                     /locus_tag="VFU45_01140"
     CDS             complement(615..1001)
                     /locus_tag="VFU45_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5357693.1"
                     /translation="MARQPNGFTLIELLIVVVIIGILAAIAIPKYNASKGEAMVATMK
                     SDLRNLAVAEEAYAYDHYTYYGGTDLTGQLNMHVSGNNVITIAHADSSGWGATATNPA
                     ATKTCALFVSGAAVAPATVAGVLACS"
     gene            complement(1119..1487)
                     /locus_tag="VFU45_01145"
     CDS             complement(1119..1487)
                     /locus_tag="VFU45_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5357694.1"
                     /translation="MSNRKGFTLIELLIVVVIIGILAAIAIPKFAATKDKAKLASVKS
                     DLRNLMTAEESYFSDNSTYTSALATSIFAVSTGNAYTAAVTGTSGYAATVSNSSISTG
                     STSCTVNVGAGSTNDGVINC"
     gene            complement(1757..2125)
                     /locus_tag="VFU45_01150"
     CDS             complement(1757..2125)
                     /locus_tag="VFU45_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5357695.1"
                     /translation="MSGRRGFTLIELLIVVVIIGILAAIAIPKFAATKDKAKLASVKS
                     DIHNLMTAEESYVADNGTYTSALPTTMFATSTGNACTAAVTGTSGYAATVSNSSISTG
                     TTSCTVNVGGGATNSGVISC"
     gene            2251..3987
                     /locus_tag="VFU45_01155"
     CDS             2251..3987
                     /locus_tag="VFU45_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357696.1"
                     /translation="MNIPEGASPPPGRTRLGGPALVLLAALAMGGVVLHRFWLQDEIP
                     IIEHYRMIHGATGLWLPFLKPYWPPPFSPDLYRPLSLVFFTLQWAAGDGAIWLFHLLN
                     LVLYILCVTATWRLARQMLPAGAAWAAAALFAVHPVHVEAAALSINQSETLVALLIIS
                     AVGVYVDWRRAPDRHPRAPWVVLALYLVSCLVKESGIVLPGLLLAAELTILDDGTPLV
                     RRAVRLRPWALGMMLIASAFIGVRTLVLHGFVGSFLAEGLFGLGWSGRVLTMLGVVPR
                     WAALTLWPAHLQEDYSPREIVGTNHWGTDQTLGLLILLVTLVLLVRSWRRNKAAAFGL
                     LWFFVAIFPVSNVVVPTGIVLAERTLFLPSVGVALALGGLVAGLVARYGAGAGLLLRR
                     AAPAALALVLVLGVVRSFTRMKVWHDPRSLWGQSMLDAPESFRVNEAFAYLMQRIGRN
                     DRFVYYMRQAIALAGRSARLNTALGDWYRNHNDCTTALPLYAEALRIDPEDEVTRASS
                     LACLFSRGKYRQARGVATVGRGYLPDSRVLARFQVLSESLAVADPAGRGVKLTIDSLA
                     PALLLVTGPAAR"
     gene            complement(4000..4704)
                     /locus_tag="VFU45_01160"
     CDS             complement(4000..4704)
                     /locus_tag="VFU45_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5357697.1"
                     /translation="MTASDAPDITVVLMTYNEQATVAEVAAELSGALEALGRPWELLI
                     VDDGSTDGSGPVAVQLAAADDRVRVVRHGVNGGLGAVYRTGFQAARGRHVTFFPADGQ
                     FPADILHTFRPAMDECDLVLGFLPERRGAPLSRFLSAAERATYRVLLGPMPRFQGVLM
                     YRRELIEAMTLRSDGRGWAVVMEFVLRVSRGPYRVVSLPTSVRPRKAGRSKVNNLRTI
                     LANVRQVLPLRRLVRS"
     gene            complement(4701..5354)
                     /locus_tag="VFU45_01165"
     CDS             complement(4701..5354)
                     /locus_tag="VFU45_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEU5357698.1"
                     /translation="MIPFLILGAGGTARDVLEWLPALAAAGRPHRCIGVLDDDPAKLG
                     NDIGGARVVGALPDAVRWPDALVLDALGGPRSHRRRAEMIGRTGLTRDRFFTLVHPTA
                     VVAGSAVIGVGALVYPFTWIGPGVRLADHVTVLSHGSVNHDAEVGTGTILASHVMLAG
                     GVMVEDHCYLGAGARVRQGVRVGAGAMVGMGSVVVGDVPAGATVAGNPARPLRSAGA"
     gene            complement(5351..6487)
                     /locus_tag="VFU45_01170"
     CDS             complement(5351..6487)
                     /locus_tag="VFU45_01170"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5357699.1"
                     /translation="MVSGADEPLIRLARPDIDDADIAAVAEVLRSGFLVQGPQVQAFE
                     SRIAEMAGAAHGVAVANCTAALQLALLVLDVGPGDAVAVGAYSWPASANVIALVGAEP
                     VFVDIDPVSWTMRPDALKTALATHPHVKAVMPVHVFGAMADLPAICALAESRRLPVVE
                     DAACALGASLAGRPAGGWGRVGCFSFHPRKSVTTGEGGVLVTSDPAVARRARALRNHG
                     LDPDAAAPDFILPGYNLRLTEMQGALGVSQLRKFTRLLDRRREVAGWYAEALAGSDVT
                     LQGAPAPEAHAYQAYVVLLPRGVQRDSAIAALRQAGIETTIGTHHQPLTSWFRTRGRH
                     RPGDFPVTDDVAARALALPLHSALTRRDVDRVARALTQVLRGPR"
     gene            complement(6460..7422)
                     /locus_tag="VFU45_01175"
     CDS             complement(6460..7422)
                     /locus_tag="VFU45_01175"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5357700.1"
                     /translation="MTISGKRVLLTGGAGFIGSRLAARLLADNEVVVFDSGHRDSLRD
                     TAASAHRNLTLIRGDVLDAAALTAAARGCQAVVHLAAIAGVDTVIKMPVETMKVNILG
                     THNALEAARALPGLERFIDFSTSEVFGAFAFKVREGDATSLGAVGEARWTYAVSKLAT
                     EHLAFSYHRAYGVPAVAVRPFNIYGPGQVGEGAIHRFIVQALRNEDITIHNDGSQIRA
                     WCYVDDMVDGILAALERPEAVGHTFNIGNPRSVLTIYQLAQDVVRLSGSTSRLTFKPW
                     GSTEVELRIPNIDKARELLGYEPKVDLAEGLMRTIEWYRARTSR"
     gene            7494..>7829
                     /locus_tag="VFU45_01180"
     CDS             7494..>7829
                     /locus_tag="VFU45_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357701.1"
                     /translation="MTDPLPPASHRSTFALGAGAAAAAVLALLLYRPWEGRPFAILDF
                     SEFLPLLKGQGGFVARLGALVHYYLFEHGRLNLASYVALSAKWTVLGAEPTRWQWARF
                     VEMGALVGGV"
BASE COUNT         1112 a         2707 c         2862 g         1148 t
ORIGIN      
        1 cgatggtccg gaccagtgcc gctgttccgg caagggcacc ggccgccacc gcccatccga
       61 gacggccggc cgtggcgccg ctccggtccg ccatccagag aaccaggacc aggaggagga
      121 ggaagcacgc ttcggagagg agcagcgtcg acaccgccag gaccggtgtc gcgacggcgc
      181 cgaggacggc ggcgggtgct gcgatccacg gcgcgatgcc gaaacggagt cgaagggcgg
      241 cggcgaccag cgctgcggcg atggcgaggg cgccggcgtt gatccactcc ccccaccggg
      301 caacggcgtc caccgacccg ccgagacgcc agagcatggc gaggaggagc ggatagcccg
      361 gcgggtagtg cgatggcgcc ggtgccccgg gccagccgat gtagctgagg ccgtgcccgg
      421 tggcgagggc gcggccgagg atggcgtaga agccgtcgtc gatgtagacg ccgaccggcg
      481 ccccggtgcg ggcccagcgc gcgacgacga gcaccgccag gccggcgatg gaggcggcga
      541 ggatcgcgga acgccggatg gtcatgcggg gactgcggga aggggcgcgc gcgaactcgc
      601 agccgctgga gcggtcagga acaggcgagg acgccggcca ccgtggcggg cgccacggcc
      661 gcgccgctca cgaagagggc gcaggtcttg gtggcggcgg ggttggtcgc cgtggcgccc
      721 cagccgctcg aatcggcgtg ggcgatggtg atgacgttgt tgccggagac gtgcatgttc
      781 agctggccgg tgaggtcggt gccgccgtag tacgtgtagt ggtcgtaggc atacgcctct
      841 tccgccacgg ccaggttccg gaggtcggac ttcatcgtcg cgaccatcgc ctcgcccttg
      901 ctggcgttgt acttcgggat cgcgatggcg gcgaggatgc cgatgatcac gaccacgatc
      961 aggagctcga tgagggtgaa tccgttcggc tgtcgggcca tcgatccact ccgtccggcc
     1021 ggaggatgcg ggcaacgcag ggggacgaaa gccgaccggc ctggccgtgc cgaggggctg
     1081 gaccaggccg gcggacttcc gctcccgatg ctacgggatc agcagttgat gacgccgtcg
     1141 ttcgtggagc cggcgccgac gttcacggtg caggacgtgg acccggtcga gatcgagctg
     1201 ttcgacacgg tggccgcgta gccgctggtg ccggtgaccg ccgccgtgta ggcgttgccg
     1261 gtcgagacgg cgaagatgct ggtggccagc gcgctggtgt aggtcgagtt gtcggagaag
     1321 tagctctcct cggccgtcat caggttgcgc aggtcggact tgaccgacgc gagcttggcc
     1381 ttgtccttgg tcgccgcgaa cttcgggatc gcgatggcgg ccaggatgcc gatgatcacg
     1441 accacgatca gcagctcgat gagcgtgaaa cccttgcggt tggacacggg aactccggtg
     1501 aattggggag ggaacggtcc caggcggtcc tggattcacc cctccacagg gcaatgccgg
     1561 ggccacctgg agcgcaattc ccaactgcct accgggcaac atcttgcggc gttcggccgc
     1621 ggggacacta aggaaatgaa ggcgatacaa gcgggcattc cgcgaatatc gggccgtcta
     1681 cgatggtcgc ccgtggcggg ccggggcgtg tatagattgc ggtgcgccgg cgcgccggcc
     1741 gccccgcgga ccaggctcag cagctgatga cgccgctgtt ggtggccccg ccgccgacgt
     1801 tgacggtgca ggaggtcgtc ccggtcgaga tcgagctgtt cgacacggtg gccgcgtagc
     1861 cgctggtgcc ggtgaccgcc gccgtgcagg cgttgccggt cgacgtggcg aacatcgtgg
     1921 tcgggagcgc gctggtgtac gtgccattgt cggcgacgta gctctcctcc gccgtcatca
     1981 ggttgtggat gtcggacttg accgacgcga gcttggcctt gtccttggtc gcggcgaact
     2041 tcgggatggc gatcgcggcc aggatgccga tgatcaccac gacgatcagc agttcgatga
     2101 gcgtgaagcc tcggcggccg gacatttgcg tctcctcatg ggggcgccac ggaccacgcc
     2161 gcggcagggg acgacgacct tcattctgac cacaatctac cgaacgaatg gcggaatgcg
     2221 atggcctcac cgcactccag tgcacccggc gtgaacatcc ccgaaggcgc ctccccgcct
     2281 cccgggcgga cccgcctcgg aggcccggcg ctcgtcctcc tcgccgcgct ggcgatgggg
     2341 ggcgtcgtgt tgcaccggtt ctggctccag gacgagatcc cgatcatcga gcactaccgg
     2401 atgatccacg gcgccaccgg gctctggctg ccgttcctca agccctactg gccaccgccc
     2461 ttcagccccg acctgtaccg tccgctctcg ctggtgttct tcaccctgca atgggcggcg
     2521 ggcgatggcg ccatctggct cttccacctg ctcaacctgg tgctgtacat cctgtgcgtc
     2581 accgcgacct ggcgcctggc gcggcagatg ctcccggccg gggcggcgtg ggcggccgcg
     2641 gcgctcttcg cggtgcaccc ggtccacgtg gaggcggcgg cgctctcgat caaccagagc
     2701 gagacgctgg tggcgctcct catcatctcg gccgtcggcg tctacgtcga ctggcgccgc
     2761 gcacccgacc ggcacccgcg ggcgccgtgg gtcgtgctgg cgctctacct ggtgtcctgc
     2821 ctggtcaagg agagcgggat cgtcctgccg ggcctgctgc tcgccgccga gctcacgatc
     2881 ctcgacgacg gcacgcccct ggtccggcgc gcggtgcggc ttcgtccgtg ggcgctgggg
     2941 atgatgctca tcgcctcggc gttcatcggc gtacggacgc tcgtcctcca cggcttcgtc
     3001 ggcagcttcc tggccgaggg acttttcggg ctggggtgga gcggacgggt gctcacgatg
     3061 ctgggcgtgg tgccgcgatg ggcggcgctg accctctggc cggcgcacct gcaggaggac
     3121 tactcccccc gcgagatcgt cggcaccaac cactggggca ccgaccagac cctgggcctg
     3181 ctgatcctcc tcgtgacgct ggtgctcctg gtgcggagct ggcgccgcaa caaggcggcc
     3241 gcgttcggcc tgctctggtt cttcgtggcg atcttcccgg tgagcaacgt ggtggtgccg
     3301 accggcatcg tgctggcaga gcggacgttg ttcctgccga gcgtgggcgt ggccctggcg
     3361 ctgggcgggc tggtggcggg gctcgtggcc cggtacggcg ccggggcggg gctcctgctg
     3421 cgccgggcgg cgcccgcggc gctcgctctg gtgctggtcc tgggcgtggt ccggagcttc
     3481 acccggatga aggtctggca cgacccgcgc tcgctctggg gccagtcgat gctcgacgcg
     3541 ccggagagct tccgcgtcaa cgaggcgttc gcctacctga tgcagcggat cgggcggaat
     3601 gaccggttcg tgtactacat gcgccaggcc atcgcgctgg cgggccggag cgcccggctc
     3661 aacaccgccc tgggcgactg gtaccgcaac cacaatgact gtacgaccgc gctcccgctc
     3721 tatgccgagg cgctccggat cgacccggag gacgaggtga cccgtgcgtc gtcgctcgcg
     3781 tgcctgttct cgagggggaa gtaccggcag gcacggggcg tggccaccgt gggccggggc
     3841 tacctccccg acagccgggt cctggcccgg ttccaggtgc tgagtgaaag cctcgcggtg
     3901 gccgaccccg ccgggcgggg ggtcaagctc acgatcgact cgctggcgcc cgcactgctg
     3961 ctcgtgaccg gccccgcggc gcgctgaccg cgagaccgtt caggaccgga ccaggcgtcg
     4021 gagcgggagg acctggcgca cattggccag gatggtgcga aggttgttga ccttggagcg
     4081 cccggccttc cgaggccgga cgctcgtggg aagtgaaacg acgcggtacg gcccccgcga
     4141 cacccgcagc acgaactcca tcaccaccgc ccagccccgc ccgtccgacc gcaacgtcat
     4201 cgcctcgatc agctcccgcc ggtacatcag gaccccctgg aacctgggca tcggccccag
     4261 gagcacgcga tacgttgccc gctccgccgc cgacaggaac cgcgagagcg gcgccccgcg
     4321 gcgctccggg aggaacccga gcaccaggtc gcactcgtcc atcgcgggcc ggaacgtgtg
     4381 caggatgtcg gccgggaact ggccgtcggc ggggaagaac gtcacgtggc ggccgcgtgc
     4441 agcctggaac cccgtgcgat acaccgcgcc cagcccgccg ttcacgccgt ggcgcaccac
     4501 gcggacccgg tcgtcggcag cggccagttg cactgcgacc gggccgctgc cgtcggtgct
     4561 cccgtcgtcg acgatgagga gttcccacgg ccgccccagc gcctcgagcg cgccggagag
     4621 ctccgccgcg acctcggcca ccgtcgcctg ctcattgtag gtcatcagga cgacggtgat
     4681 gtcaggcgcg tccgaagccg tcacgcgccg gcgctccgca ggggccgcgc gggattcccg
     4741 gcgacagtgg caccggccgg gacatcgccg accaccacgc tgcccatccc caccatcgcg
     4801 ccggcgccca cccgcacgcc ctggcgcacc cgggcgccgg cgccgaggta gcagtggtcc
     4861 tccaccatca cgccgcccgc cagcatcacg tgcgaggcaa ggatggtccc cgtccccact
     4921 tcggcgtcat ggttcacgct gccgtgggag aggacggtca cgtggtccgc caggcggacg
     4981 cccggaccga tccaggtgaa cgggtagacc agcgccccga cgccgatgac cgcgctcccg
     5041 gcgacgacgg cggtcgggtg caccagcgtg aagaaccggt cgcgcgtgag cccggtgcgc
     5101 ccgatcatct cggctcggcg ccggtggctt cggggaccgc cgagcgcatc caacaccagg
     5161 gcatccggcc agcgcacggc gtccggcagc gcgccgacca cccgcgcgcc cccgatgtcg
     5221 ttcccgagct tggccgggtc gtcgtcgagc acgccgatgc accggtgggg ccgaccggcg
     5281 gcggccagcg ccggcagcca ctcgaggacg tcccgggcgg tgccgccggc gccgaggatc
     5341 aggaacggga tcatcgcggg cctcgcagga cctgcgtgag ggcgcgtgcg acgcggtcga
     5401 cgtcgcggcg ggtgagggcg gaatggagcg gcagcgcaag cgcgcgcgct gcgacgtcgt
     5461 cggtgaccgg gaagtcgccg gggcggtggc ggccgcgggt ccggaaccag ctggtgagcg
     5521 gctggtggtg ggtcccgatc gtcgtctcga tgccggcttg gcgaagtgcc gcgatggcgc
     5581 tgtcacgctg cacaccgcgg ggaagcagca caacgtacgc ctggtaggcg tgcgcttcgg
     5641 gcgccggcgc cccctgcagc gtcacgtcac tcccggccag cgcctcggca taccagcccg
     5701 cgacctcgcg tcgacggtcc aacaggcggg tgaacttccg caattgggag acgcccaggg
     5761 cgccctgcat ctcggtgagg cgcaggttgt agccggggag gatgaagtcc ggggcggcgg
     5821 cgtcgggatc gagcccgtgg ttccggagcg cgcgcgcccg gcgggccact gcggggtcgg
     5881 aggtcacgag cacgccgccc tcgccggtgg tgacgctctt ccgcgggtgg aagctgaagc
     5941 agcccacgcg accccatccc cccgcgggcc gccccgcaag cgaggcgccg agcgcgcagg
     6001 ccgcatcctc gaccacgggg aggcgccgcg actccgcaag ggcgcagatg gcggggaggt
     6061 cggccatcgc cccgaagacg tgcaccggca tgaccgcctt cacatgcggg tgggtggcga
     6121 gcgccgtctt gagggcatcg ggtcgcatcg tccaggacac ggggtcgatg tcgacgaaca
     6181 ccggctcggc accgaccagg gcgatcacgt tggccgaggc gggccaggag taggcgccga
     6241 ctgccacggc atcgccgggc ccgacgtcca ggacgaggag cgcgagctgc agcgcggcgg
     6301 tgcagttggc caccgccacg ccgtgggccg cgccggccat ctccgcgatc cgggactcga
     6361 acgcctggac ctgcggcccc tggaccagga agccgctccg aaggacctcg gccaccgcgg
     6421 cgatgtccgc gtcgtcgatg tccggccgcg ccaggcggat cagcggctcg tccgcgcccg
     6481 ataccattcg atggtccgca tcaggccttc ggcaaggtcc accttcggct catagccaag
     6541 gagttcgcgc gccttgtcga tattgggtat ccggagttcg acctcggtgc tgccccacgg
     6601 cttgaacgtg agccgggacg tggagccgct gaggcggacc acgtcctgcg cgagctggta
     6661 gatggtcagc acgctccgcg ggttcccgat gttgaacgtg tggcccacgg cctccgggcg
     6721 ctcgagcgcg gccaggatgc cgtccaccat gtcgtcgacg tagcaccagg cgcggatctg
     6781 gctgccgtcg ttgtggatcg tgatgtcctc gttgcggagc gcctggacga tgaagcggtg
     6841 gatggcccct tcgcccacct ggccggggcc atagatgttg aacggccgca cggcgaccgc
     6901 ggggacgccg taggcccggt gatagctgaa cgccaggtgt tcggtggcga gcttgctcac
     6961 cgcgtaggtc cagcgggcct cgcccacggc gccgaggctg gtggcgtcgc cctcgcggac
     7021 cttgaaggcg aaggcgccga agacctcact ggtggagaag tcgatgaagc gttcgaggcc
     7081 cggcagcgcc cgcgcggcct ccagcgcgtt gtgggtgccg aggatgttca ccttcatcgt
     7141 ctcgaccggc atcttgatga ccgtgtcgac gccggcgatc gccgcgaggt ggaccaccgc
     7201 ctgacatccg cgggccgccg ccgtgagcgc cgccgcatcg agcacgtcgc ctcggatcag
     7261 ggtcaggttc cgatgcgcac tggccgcggt atcgcgcaga gaatcccggt ggccgctgtc
     7321 gaacacgacc acttcgttgt cggcaaggag ccgcgcagca aggcggctgc cgatgaagcc
     7381 ggccccgccg gtcaggagga cacgcttacc gctgatcgtc acgcagggat cccggatgga
     7441 gggtggcatc gcccgaaacg cccgcggcgg cgccgtcggc tataatatac cggatgaccg
     7501 acccactgcc gccggcgtcg caccgctcga ccttcgcgct cggcgccggc gcagcggccg
     7561 ccgccgtact ggccctcctg ctctaccgcc cgtgggaagg gcgccccttc gcgatcctcg
     7621 atttctccga gttcctgccg ctcctgaagg gccagggcgg attcgtcgca cgcctgggcg
     7681 ccctcgtcca ctactacctg ttcgagcacg gccggctcaa tctcgcatcg tacgtcgccc
     7741 tttcggccaa gtggaccgtc ctcggcgccg aaccgacgcg gtggcagtgg gcgcggttcg
     7801 tcgagatggg cgccctggtg ggcggggtg
//
LOCUS       DASUAP010000048         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1132199, whole genome shotgun sequence.
ACCESSION   DASUAP010000048 DASUAP010000000
VERSION     DASUAP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132199"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFU45_01185"
     CDS             <1..556
                     /locus_tag="VFU45_01185"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEU5357702.1"
                     /translation="WSPDGKWLVVRTDNGTAGAGDIVGVRTSGDTTPVPLAASRFTEL
                     SPAVSPDGRWLAYASNETGANEVYVRRFPDSEGGQRQVSAHGGAEPRWSRDGQTLYFL
                     DDDSRLMAAQVRTTPEFEVAAIKPLFDAPQFIRDGFHQSYEVAPDGRSFLFLHQRPGP
                     SGAQGPRPVLVQHWLSDLKAPQAR"
     gene            647..>2784
                     /locus_tag="VFU45_01190"
     CDS             647..>2784
                     /locus_tag="VFU45_01190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5357703.1"
                     /translation="MDLQSQLQAHLGKTFVIERELGGGGMSRVFVADEVRLGRKVVIK
                     VLSPDLAQGISVQRFEREILLAASLQQANIVPVLSAGQVDNLPWFTMPFVEGESLRAR
                     LALGPLPIADVLSVLRDVTKALAYAHARGIVHRDIKPDNVLLSGGTAVVTDFGIAKAL
                     SASRGANSGVTLTQLGTAIGTPAYMAPEQVAGDPNLDHRVDLYALGCVAHELLTGQAP
                     FSDRTPQKVLAAHLSEAAVSVSTLRVDCPPALADLVTRLLRKDPADRVQTAVEVLHDL
                     DAVGTTSSQRAALSAPGMLPKALGLYVLAVGAAAILAKAAVVGIGLPDWVFPGTMVVM
                     LLGLPALLATAYIKRVARQAVTATPTLTPGGTSVPKLPGGTIATLALKANPHVSWRRT
                     ARGGVYAMGAFVAAIVAFMTLRALGIGPWGSLLANGTLAQNARVVVGSFTAAPDDSSL
                     APIVGEAVRAALSQSTAIRVLDPAQVAPILVQMRRNPQAPLDPATAREVATRAGAAAV
                     VGGRLARAGTGYAVSVELTNAADGASLASYQGTADGPKDLLAVVDGLAKKLRARVGES
                     LRRVQNAVPLQQATTSSLEALRLYSEAAQANDVDQDFARAIQRARQAVAIDSTFALAW
                     RKLAVAYVNSAAGVSAVDSALDHAMRYADKLPDREKYLVRGFYYEQHHANADPAKALA
                     AYQAVLAIDRTHTTAPPELVRLYGARGEYD"
BASE COUNT          373 a         1138 c          900 g          373 t
ORIGIN      
        1 ctggtcgccc gacggcaaat ggctggtggt ccgcaccgac aacggcaccg ccggcgcggg
       61 cgacatcgtc ggtgtccgca ccagcggcga cacgaccccg gtcccgctcg cggcctcgcg
      121 gttcacggag ctgagcccgg cggtctcgcc cgacggccgc tggctggcgt acgcctcgaa
      181 cgagaccggg gccaacgagg tctacgtccg ccgcttcccc gacagcgagg gggggcagcg
      241 ccaggtctcg gcccacggcg gcgccgagcc ccgctggtcg cgcgacggcc agaccttgta
      301 cttcctcgac gacgacagcc gactcatggc cgcccaggtc cggacgacgc cggaattcga
      361 ggtcgcggcg atcaagccgc tgttcgacgc accgcagttc atccgggacg gcttccacca
      421 gtcgtacgag gtggcacccg acgggcgatc cttcctcttc ctgcaccagc ggcccggccc
      481 ctcgggcgcc cagggcccgc ggccggtcct ggtccagcac tggctgagcg acctcaaggc
      541 ccctcaggca cgctgaccgg agggggcgcc ggcgctggcg tcccccaccg ccccaccgta
      601 agtttcccgc tcctgcccgc aggtgcacac ccgctcgagg accgacttgg acctccagtc
      661 gcagctgcaa gcccatctcg gcaagacctt cgtcatcgag cgcgagctcg gcggcggcgg
      721 catgtcgcgc gtcttcgtgg ccgacgaggt gcgcctcggc cgcaaggtgg tcatcaaggt
      781 cctgagcccc gacctggccc agggcatcag tgtccagcgc ttcgagcgcg agatcctcct
      841 cgccgcctcg ctgcagcagg ccaacatcgt ccccgtcctc tccgccggcc aggtggacaa
      901 cctcccctgg ttcacgatgc cgttcgtcga gggcgaatcg ctccgggcgc gcctggccct
      961 gggcccgctc cccatcgccg acgtcctctc cgtcctgcgc gacgtcacca aggccctcgc
     1021 ctacgcccac gcccgcggga tcgtccaccg cgacatcaag ccggacaacg tgctcctctc
     1081 cggcggcacc gccgtcgtca ccgacttcgg gatcgccaag gcactctccg cctcgcgcgg
     1141 cgccaactcc ggcgtcaccc tcacgcagct cggcaccgcc atcggcaccc cggcctacat
     1201 ggcccccgag caggtggccg gcgacccgaa cctcgaccac cgggtggacc tctacgccct
     1261 cggctgcgtg gcccacgaac tcctgaccgg ccaggcgccc ttctccgacc gcaccccgca
     1321 gaaggtcctc gccgcgcacc tgagcgaagc cgcggtgtcg gtgtcgacgc tccgggtcga
     1381 ctgcccgccg gcgctcgccg acctggtgac ccgcctcctc cggaaggacc ccgccgaccg
     1441 ggtgcagacg gcggtcgagg tgctgcacga cctcgatgcg gtcggcacca cgtcgagcca
     1501 gcgcgccgcc ctctccgcgc ccgggatgct ccccaaggcg ctcggcctct acgtcctcgc
     1561 cgtcggcgcg gcggcgatcc ttgccaaggc cgcggtcgtc ggcatcgggc tccccgattg
     1621 ggtcttcccc ggcacgatgg tggtgatgct cctggggctc ccggcgctcc tcgccaccgc
     1681 gtacatcaag cgggtggccc gccaggccgt caccgccacg cccacactca cccccggggg
     1741 cacctcggtg ccgaagctcc ccggcgggac catcgccacc ctggcgctca aggccaatcc
     1801 ccacgtgtcg tggcgccgca ccgcgcgcgg cggcgtgtac gcgatgggcg ccttcgtggc
     1861 ggccatcgtc gccttcatga cgctgcgcgc gctcggcatc ggcccgtggg gttcgctcct
     1921 cgccaacggg acgctggccc agaacgcgcg cgtcgtggtc ggaagcttca ccgccgcccc
     1981 cgacgattcc tcgctcgccc cgatcgtggg cgaggcggtg cgcgccgcgc tttcgcagtc
     2041 caccgccatc cgggtgctcg atccggccca ggtggcgccg atcctcgtgc agatgcggcg
     2101 gaatccgcag gccccgctcg accccgccac cgcgcgcgaa gtggccaccc gcgccggcgc
     2161 ggccgccgtg gtcggcgggc ggctggcccg ggccggcacc ggctacgcgg tgagcgtcga
     2221 actcaccaac gccgccgacg gcgcctcgct cgcctcctat cagggcaccg ccgacggacc
     2281 gaaggacctg ctcgcggtgg tggacgggct cgccaagaag ctccgcgccc gggtcggcga
     2341 gtcgctccgc cgggtgcaga acgcggtccc gctccagcag gccaccacca gctcgctcga
     2401 ggcgctccgg ctctacagcg aggccgccca ggccaacgac gtggaccagg acttcgcccg
     2461 cgcgatccag cgggcccgcc aggcggtcgc gatcgactcg acctttgcgc tcgcctggcg
     2521 gaagctcgcc gtggcgtacg tgaacagcgc cgccggcgtg agcgcagtgg attcggccct
     2581 cgaccatgcc atgcgttacg ccgacaagct tcctgaccgt gagaagtacc tggtccgcgg
     2641 tttctactac gaacagcacc acgccaacgc cgacccggcc aaggccctcg ccgcctacca
     2701 ggcggtgctc gccatcgacc gcacccacac cacggccccc cccgagctgg tgcggctcta
     2761 cggcgcccgc ggcgagtacg acag
//
LOCUS       DASUAP010000049         4629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_805233, whole genome shotgun sequence.
ACCESSION   DASUAP010000049 DASUAP010000000
VERSION     DASUAP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805233"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..306)
                     /locus_tag="VFU45_01195"
     CDS             complement(34..306)
                     /locus_tag="VFU45_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357704.1"
                     /translation="MSKKKPRRPTTGPSALDQARDELFSHILRCGVMEAEPEHQKDWF
                     DDTMLYLAERYEELTPEELAQVRTLGERYCQPVVSRLPQTVGASAE"
     gene            460..762
                     /locus_tag="VFU45_01200"
     CDS             460..762
                     /locus_tag="VFU45_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357705.1"
                     /translation="MSEHDQVYGDERERRLRERLIEVAARIRELEVRGELLASVPELL
                     RLLGEARSELFHYEVRVTYDTPELAEHRKLVERAAEGWTPDDEGDDELPWRNRRVD"
     gene            738..1445
                     /locus_tag="VFU45_01205"
     CDS             738..1445
                     /locus_tag="VFU45_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5357706.1"
                     /translation="MAQPTGGLNRFYAVLAALAVAGLAVIGWLVLRKPPLSIPANVVV
                     TAADTTGFTGYYLGSDSAPVTITEYGDFECPLCASWDGVQFPAVREQLINTGKVRWRF
                     RDWPIDGLHRWTRVASHAAACADEQGKYWPMHEALFVWQSKWAFSSDAPSLIHDYAVQ
                     AGLDGGKFDACMASKKYAGRIQASFNEGEKLGVNATPSFLIGGRLYVGVEHMSSDEIG
                     KLVDSIIAAMPKPAKKP"
     gene            1442..1873
                     /locus_tag="VFU45_01210"
     CDS             1442..1873
                     /locus_tag="VFU45_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEU5357707.1"
                     /translation="MKHRMAVGLLSLAGFFLALYLYLYKIGLIGTLACGTGGCETVQM
                     SPWSRFLGVEVALYGVIGYALLFGLALVSLQAGEARRHWSDRLVLLGAGAGVLFTAYL
                     TSLELFVIHAICRWCVGSAAIITSIFVVAFMGRRAAAGTTG"
     gene            1879..3792
                     /locus_tag="VFU45_01215"
     CDS             1879..3792
                     /locus_tag="VFU45_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEU5357708.1"
                     /translation="MRTDARREPVGVALAGLSLGALGVVYGDIGTSPLYALTECFRGV
                     HSVEPTRDNIFGVLSLVFWALTFVVTFKYLLFVMRADNRGEGGILALFALVRPRGSAH
                     GRAWVIVGLGLFGAALLYGDGVITPAITVLGAVEGVSVATPAFQHLVVPLSVAILVAL
                     FWFQKRGTAAIGRVFGPVMLVWFLSITALGVRGILMYPAILRALDPRLAIELFLRDGT
                     AGFLVLGGVVLVVTGAEALYADMGHFGKRPIRVAWAALVLPALVLNYFGQSANLLVHP
                     EAIRNPFYSLVPDWALYPMILVATLAAIVASQALISGAFSLTRQAVQLGYAPRVTIVH
                     TSSTEIGQIYIPEVNSALAVACVALVLGFRSSSALAAAYGIAVTGTMIITSVLFHQVA
                     VRRWKWPEWQATALTTLFLVVDLAFFGANVVKIGDGGWFPLGVAAVVYTLMTTWKKGR
                     DVLGAFSRENSLPMGLFLDDVRRRRPARVPGTAVFMSSDPSGAPPVLLHHLKHNKMLH
                     ERVILVSLTSEEIPQVPDRERVACTDLGEGFFQVVARYGFMETPNVPELLHSLEQRGN
                     VDAMGLGGIVVRPLDTTFYLGRETLLPDGPSRMARWRKRLFIVMARNAVSASSFFALP
                     ANRVVEMGAQIQL"
     gene            complement(3933..>4629)
                     /locus_tag="VFU45_01220"
     CDS             complement(3933..>4629)
                     /locus_tag="VFU45_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HEU5357709.1"
                     /translation="ETGGIMITPLPGITTTKPGSATVPFPGVQVALVDATGKALSTGG
                     GFLVLTAPWPGMLRTIYGDDERYRQTYWSRFPGRYFAGDGAKVDDDGYWWILGRVDDV
                     LNVAGHRIGTMEVESALVDHPAVAEAAVVGKAHELKGQALAAFVTLKEGRHPTAELKD
                     DLREHVAKKIGAIARPDDLFFTADLPKTRSGKIMRRLLRDIAEGRALGDTTTLADPAV
                     VARLKDQYESEES"
BASE COUNT          623 a         1584 c         1615 g          807 t
ORIGIN      
        1 ggacgccgtg tggacttccg ggaccgcggt ggcctactcg gccgacgcgc cgaccgtctg
       61 gggcagccgc gagaccaccg gctggcagta ccgctcgccc agggtgcgga cctgcgccag
      121 ttcctccggc gtcagctcct cataccgctc ggcgaggtac agcatcgtgt cgtcgaacca
      181 atccttctgg tgctcgggtt ccgcttccat cactccgcag cgcaggatgt ggctgaagag
      241 ttcgtcgcgc gcctggtcga gggcggacgg gccggtggtc ggacgccgag gcttcttctt
      301 ggacattaac ggcgctcctt gcgcgtaatg gggtcgtaca ccgcagcaaa tatacaaagg
      361 aagggcgccg gaatgcagtg gggcgcgccg ccgcgccggg tcggcgcccg cgattagatt
      421 ccgggtcgcg gccgatcgta cggccgggga ggcgcggcga tgtcggagca cgaccaggtg
      481 tacggcgacg agcgggagcg ccggctgcgc gaacggctca tcgaggtcgc cgcgcggatc
      541 cgggagctgg aggtgcgggg cgagctgctg gcgagcgtgc cggagctcct ccggttgctc
      601 ggcgaggcgc gcagtgagct gttccactat gaagtgcggg tcacctacga caccccggag
      661 ttggcggagc accgcaagct ggtggagcgg gccgccgagg ggtggactcc tgacgacgaa
      721 ggggacgacg aactgccatg gcgcaaccga cgggtggact gaaccggttc tatgcagtgc
      781 tggcggcgct ggcggtggcc ggccttgcgg tcatcgggtg gctggtgctg cggaagccgc
      841 cgctcagcat cccggccaac gtggtggtca ccgcggccga cacgaccggg ttcacggggt
      901 attacctcgg gtcggactcc gcgccggtga ccatcaccga gtacggcgac ttcgagtgcc
      961 cgctctgcgc cagctgggac ggggtgcagt tcccggcggt gcgcgagcag ctcatcaaca
     1021 ccggcaaggt gcgctggcgg ttccgtgact ggcccatcga cgggctgcac cgctggaccc
     1081 gggtggcatc ccacgccgcg gcgtgcgccg acgagcaggg caagtactgg ccgatgcacg
     1141 aggcgctctt cgtctggcag agcaagtggg ccttctcgtc ggatgcgccg tcgctcatcc
     1201 acgactatgc cgtgcaggcg ggccttgacg gcggcaagtt cgatgcctgc atggcctcca
     1261 agaagtacgc cgggcgtatc caggcctcgt tcaacgaggg cgagaagctc ggcgtgaacg
     1321 cgacgccgag cttcctgatc gggggccggc tctacgtcgg cgtcgagcac atgagttcgg
     1381 acgagatcgg caagctggtc gactcgatca tcgcggcgat gccgaagccg gcgaagaagc
     1441 cgtgaagcac cggatggccg tggggctcct gagcctcgcc gggttcttcc tggccctgta
     1501 cctgtacctc tacaagatcg ggctcatcgg cacgctggcc tgcggcaccg gcgggtgcga
     1561 gacggtgcag atgagcccgt ggagccggtt cctgggcgtc gaggtggcgc tgtacggggt
     1621 catcggctat gcgctgctct tcgggctcgc gctcgtgtcg ctccaggccg gcgaggcgcg
     1681 gcggcattgg agcgaccggc tcgtcctcct cggcgccggc gccggcgtcc tcttcacggc
     1741 ctacctcacc tcgctcgagc tgttcgtcat ccacgccatc tgccggtggt gcgtcggctc
     1801 ggcggccatc atcacctcga tcttcgtggt ggcattcatg ggccgccggg cggccgcggg
     1861 gaccaccggc tgaggacgat gcgcaccgac gcccgccgtg agcccgtcgg cgtcgcgctc
     1921 gccggcctgt ccctcggggc gctgggcgtg gtgtacggcg acatcggcac cagcccgctc
     1981 tacgccctca ccgagtgctt ccgcggtgtc cactccgtcg agccgacccg cgacaacatc
     2041 ttcggcgtgc tgtcgctcgt gttctgggcg ctcaccttcg tcgtcacgtt caagtatctc
     2101 ctgttcgtca tgcgggcgga caatcggggc gagggaggga tcctcgcgct cttcgccctg
     2161 gtgcgccccc gcggctcggc ccatgggcgt gcctgggtca tcgtcgggct cgggctcttc
     2221 ggcgccgcgc tcctctacgg cgatggcgtc atcacccccg ccatcaccgt cctcggcgcg
     2281 gtcgaggggg tgagcgtcgc gaccccggcc ttccagcacc tcgtggtacc cttgagcgtg
     2341 gcgatcctgg tggcgctctt ctggttccag aagcggggca ccgccgccat cggccgggtc
     2401 ttcgggccgg tgatgctggt gtggttcctc tccatcacgg cgctcggcgt ccgcggcatc
     2461 ctgatgtacc cggccatcct ccgcgccctc gacccgcgcc tggccatcga gctcttcctc
     2521 cgcgacggca ccgccggctt cctggtcctg ggcggggtgg tgctggtggt caccggcgcc
     2581 gaagcgctgt acgccgacat ggggcacttc gggaagcggc ccatccgcgt ggcctgggcg
     2641 gcgctggtgc tgccggcgct cgtcctcaac tacttcgggc agtcggccaa cctgctggtg
     2701 cacccggagg ccatccggaa cccgttctac tcgctggtac ccgactgggc gctctacccg
     2761 atgatcctgg tggcgacgct cgccgccatc gtggcgtcgc aggcgctcat ctccggcgcg
     2821 ttctcgctca cccggcaggc cgtgcagctg gggtacgcgc cgcgggtcac gatcgtccat
     2881 acctcgagca ccgagatcgg gcagatctac atccccgagg tcaacagcgc cctcgcggtg
     2941 gcgtgcgtgg cgctcgtgct cgggttccgc tcgtcgtcgg cgctggccgc ggcgtatggc
     3001 atcgccgtca ccgggacgat gatcatcacc agcgtcctgt tccatcaggt ggccgtgcgc
     3061 cggtggaaat ggccggaatg gcaggcgacg gcgctcacga cgctcttcct ggtggtggac
     3121 ctcgccttct tcggggccaa cgtcgtcaag atcggcgacg gcgggtggtt ccccctcggc
     3181 gtcgcggccg tggtgtacac cctgatgacc acctggaaga aggggcgcga cgtcctcggc
     3241 gccttctccc gcgagaactc gctcccgatg gggctcttcc tcgacgacgt gcgccgccgc
     3301 cgcccggcgc gggtcccggg cacggcggtg ttcatgagct ccgatccctc gggtgcgccg
     3361 ccggtgctgc tccaccacct caagcacaac aagatgctgc acgagcgggt gatcctcgtg
     3421 tccctcacga gcgaggagat cccccaggtg cccgaccgcg aacgggtggc ctgcaccgac
     3481 ctgggcgaag ggttcttcca ggtcgtggca cgctacggat tcatggagac gcccaacgtc
     3541 ccggaactgc tgcattcgct ggagcagcgc gggaacgtcg acgccatggg cctgggcggg
     3601 atcgtggtgc ggcccctcga taccacgttc tacctcggcc gcgaaacgct cctccccgac
     3661 ggcccgtcgc gcatggcgcg ctggcgcaag cgactgttca tcgtgatggc gcgcaacgcg
     3721 gtctcggcct cgagcttctt cgcccttccc gccaaccgcg tggtggagat gggggcgcag
     3781 atacaactct agctatcagc tatcagctat cagctaacag ctgaccgcgt tggcttggca
     3841 tcggtgtgac gagggctccc cgtatcggga gcccttgagt gtttggaacc tctgatagct
     3901 gatagccgat agctgatagc tgatagctcc ccttacgact cctcactctc gtactggtcc
     3961 ttcagccgcg ccaccaccgc cgggtcggcg agggtggtcg tgtcgcccag cgcgcgtcct
     4021 tccgcgatgt cgcgaagcaa ccggcgcatg atcttcccgc tgcgggtctt gggaaggtcg
     4081 gcggtgaaga agaggtcgtc cgggcgggcg atggcgccga tcttcttggc cacgtgctcg
     4141 cgcaggtcgt ccttgagctc ggcggtggga tggcgtcctt ccttgagcgt cacgaacgcg
     4201 gccagggcct ggcccttgag ttcatgcgcc ttcccgacga cggcggcctc cgccacggcg
     4261 ggatggtcca ccagtgcgct ctcgacctcc atcgtcccga tccggtggcc ggcgacattg
     4321 agcacgtcgt ccacccggcc gaggatccac cagtacccgt cgtcgtccac cttggcgccg
     4381 tcgcccgcga agtagcggcc gggaaaacgg ctccagtagg tctggcggta ccgctcgtcg
     4441 tcgccgtaga ttgtgcggag catcccgggc cagggcgcgg tgagcaccag gaacccgccg
     4501 cccgttgaca gcgccttgcc ggtcgcgtcg accagcgcca cctgcacgcc gggaaagggc
     4561 accgtcgccg agccgggctt ggtggtcgtg atgccgggga gcggcgtgat catgatgccg
     4621 cccgtctcg
//
LOCUS       DASUAP010000050         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_873143, whole genome shotgun sequence.
ACCESSION   DASUAP010000050 DASUAP010000000
VERSION     DASUAP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873143"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFU45_01225"
     CDS             <1..996
                     /locus_tag="VFU45_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5357710.1"
                     /translation="HKEWYHAPCPRCGKQGRRETDVSDTFLDSAWYFLRYPSVGIDDK
                     PFDPARTARWLPVTSYIGGNEHAVLHLLYSRFITMVLHDLGHVGFEEPFTRFRAHGMI
                     VKDGAKMSKSRGNVVIPDEYIAKWGADTFRTYLMFLGPLTEGGDFRDDGVSGPRRFLD
                     KLWALVGDALAGGGAAPSPGLTAKWHRAKQRVTEGMEELRYNTAIAALMELLNAVREE
                     GCHDRALVEEMILMVAPTAPHFAEECWERLGHQGSVFDARWPAFDPALAVEDSLEMVV
                     QVNGKTRGRVTVPRLAPQEAVVAAALADPAVRRFLDGKEPKKTIYVAGRLVNFVV"
     gene            complement(1105..1578)
                     /locus_tag="VFU45_01230"
     CDS             complement(1105..1578)
                     /locus_tag="VFU45_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5357711.1"
                     /translation="MPTSSFPRRRSPRLLNFPYNGPWSYFVTLITAERRPILAECTGG
                     RFRLRPIGRLLRDIWRELGEHPPAADPLDHIVMPDHFHGIVRVRSEAPGSLSRVVNGL
                     KGAVTREARRRGLLGHDPLWARSFHDRAIRSEAMLLEVQRYIAENPARWSLRRAE"
     gene            complement(1760..>2741)
                     /locus_tag="VFU45_01235"
     CDS             complement(1760..>2741)
                     /locus_tag="VFU45_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEU5357712.1"
                     /translation="DRHTEIWRKLIADAGHPVRAVRPSVRARTLAWLARRFGPGFLLP
                     MLLEEEGREVKGYLSLYKRTPAGNAQDTALLLARESAEHAGSLSGIAGRDGEPWHKTG
                     AGGFLRNLVYGFNDGLTANFGLVAGMLGAQASLGAASHAVLVAGIAGTAANALSMGSS
                     GYLAAKSEREVFEHEIAMERDEIRLMPEVERDELELIYQAKGIPAAQAKLLADDVMRD
                     PERALGEQVREELGIGEATSTPMREAWITGSATAVGSLIPVLPFFLFHGPVAIWSAFT
                     IAMLSHFGVGAARSLFTGRGIIRSGMDMFLIGLGVALVGYFVGEGIVRVL"
BASE COUNT          432 a          979 c          929 g          401 t
ORIGIN      
        1 cacaaggagt ggtaccacgc gccctgtccc aggtgcggga agcaggggcg ccgggagacc
       61 gacgtctccg acaccttcct ggattcggcc tggtacttcc tccggtatcc gtcggtcggg
      121 atcgacgaca agcccttcga cccggcccgc acggcgcgct ggctcccggt cacgtcctac
      181 atcggcggca acgagcacgc cgtgctccac ctgctctaca gccgcttcat cacgatggtg
      241 ctgcacgacc tcggccacgt cgggttcgag gagccgttca cccggttccg cgcccacggg
      301 atgatcgtca aggacggcgc caagatgtcc aagtcgcggg ggaacgtcgt catccccgac
      361 gagtacatcg cgaagtgggg cgccgacacc ttccggacct acctcatgtt cctgggaccc
      421 ctcaccgaag ggggggactt ccgggacgat ggcgtgagcg ggccgcggcg cttcctggac
      481 aagctctggg ccctcgtggg tgacgctctc gccggcggcg gcgctgcgcc ctcgccgggg
      541 ctcaccgcca agtggcaccg tgccaagcag cgcgtcaccg aggggatgga ggagctgcgc
      601 tacaacaccg ccatcgcagc gctgatggag ctcctcaacg ccgtgcggga ggaggggtgc
      661 cacgaccgtg cgctggtgga ggagatgatc ctgatggtgg cgcccacggc gccgcacttc
      721 gccgaggagt gctgggagcg cctggggcac caggggagcg tgttcgacgc ccggtggccc
      781 gcgttcgacc cggcgctcgc ggtcgaggat tcgctggaga tggtcgtgca ggtgaacggc
      841 aagacccgag gccgggtcac cgtgccccgc ttggctccgc aggaggccgt ggtcgccgcc
      901 gcgctcgccg acccggcggt ccggcgcttc ctggatggca aggaaccgaa gaagaccatt
      961 tacgtggccg gtcggctggt gaacttcgtg gtgtgatcgg gacgacgtaa ctcgagggcg
     1021 gcgcatgagg attgaaccgc atggggtggc gaagcgctag gatatcgggt cgcaggggcg
     1081 gcgcatgcgc cgcccgtacc atcctcactc agcccgccgc agtgaccatc tcgccggatt
     1141 ctccgcgata taacgctgca cctccagcaa cattgcttcg ctccggatcg cccgatcgtg
     1201 aaagctgcgt gcccacagcg ggtcgtgccc cagcagtccc cgtcgccggg cctcccgagt
     1261 gacggcaccc ttgagcccgt tgaccacccg actaagtgag cctggcgctt ccgaccggac
     1321 ccgtacgatg ccgtgaaagt gatctggcat cacgatgtga tcgagggggt cggcggcggg
     1381 cggatgctca ccgagttccc gccagatgtc gcgcaggagc cggccgatgg gccgcaatcg
     1441 gaatcggccg ccggtacact cggccaagat cgggcgacgc tcggcggtga tgagcgtgac
     1501 gaagtagctc caggggccgt tgtaggggaa gttgaggagc cgcggggaac gccggcgggg
     1561 gaacgaggaa gtaggcatcg gtcgaagttg cgcccttcct tcgtcctggc cggcatcaga
     1621 tccgcgccgg taccggcgaa atagcgcgca cctggccttc cgggcggcgc aggcgccgcc
     1681 cctgctgaat cattcgcgcc gcccctgctg aataatgtgc gccgcccccg ctcacccctc
     1741 accgctgacc gctcaccgcc tacagcaccc gcacgatccc ttcaccgacg aaatacccca
     1801 ccagcgccac gcccagcccg atcaggaaca tgtccatccc cgaccggatg atcccccggc
     1861 cggtaaagag gctccgcgcg gcgccgacgc cgaaatgcga aagcatggcg atcgtgaacg
     1921 ccgaccagat ggccaccgga ccgtggaaga ggaagaacgg gagcaccggg atgagcgacc
     1981 cgacggcggt ggcgctcccg gtgatccacg cctcccgcat cggcgtgctg gtggcctcgc
     2041 cgatgcccag ctcctcccgc acctgctcgc cgagcgcgcg ctcgggatcg cgcatcacgt
     2101 cgtcggccag gagcttggcc tgtgccgccg ggatcccctt ggcctgatag atgagctcca
     2161 gttcgtcccg ctcgacctcc ggcatcagcc ggatctcgtc ccgctccatc gcgatctcgt
     2221 gctcgaacac ctcgcgctcg ctcttggccg cgaggtagcc cgacgacccc atcgagagcg
     2281 cgttggcggc ggtcccggcg atcccggcca ccagcaccgc atggctggcg gcgccgagcg
     2341 aggcctgggc gcccagcatc cccgccacga ggccgaagtt ggcggtcagg ccatcgttga
     2401 agccgtacac caggttgcgg aggaagccgc cggcgccggt cttgtgccac ggctccccgt
     2461 cgcgccccgc gatccccgac agcgacccag cgtgctccgc ggactccctc gcgagcagga
     2521 gggccgtgtc ctgcgcgttc ccggcgggcg tgcgcttgta gagcgagagg tagcccttca
     2581 cctcccgccc ctcctcctcc agcagcatcg gcaggaggaa cccgggcccg aagcggcggg
     2641 cgagccaggc cagcgtccgc gcccgcaccg acgggcggac cgccctgacc gggtggcccg
     2701 cgtcggcgat cagcttccgc cagatctcgg tgtggcgatc c
//
LOCUS       DASUAP010000051         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_123884, whole genome shotgun sequence.
ACCESSION   DASUAP010000051 DASUAP010000000
VERSION     DASUAP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123884"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..666
                     /gene="ruvC"
                     /locus_tag="VFU45_01240"
     CDS             169..666
                     /gene="ruvC"
                     /locus_tag="VFU45_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU5357713.1"
                     /translation="MGVDPGTAIAGFGVVDGGAGGRARPSLVECGVIRTTARETLPER
                     LRVVHEGIVELIQRHRPDVVAVEGIFYARNVRSTIVLGHARGVILLAAAAAGLPVAEY
                     APAMVKRTVVGRGAAAKAQVGYMVAQLLRLAQAPSPADAADAVAIALTHLLRGGRMVG
                     PAGDR"
     gene            663..1253
                     /gene="ruvA"
                     /locus_tag="VFU45_01245"
     CDS             663..1253
                     /gene="ruvA"
                     /locus_tag="VFU45_01245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU5357714.1"
                     /translation="MIAAISGLISAREGDTVVVQTQGGVGYAITLPLGVHERLPPVGE
                     RVSLFTELVVREDGWSLYGFDDPFERTVFQRLLSASGVGPKLALAVLSALGPARAARS
                     IRDRDLAALASVTGIGKKKAERIVLELQDRFKELADAAPSAGPALAGSAAVAALAALG
                     YSAANAEAAVRAELAAGTPDETAQLVRRALQRLAGK"
     gene            1370..1546
                     /locus_tag="VFU45_01250"
     CDS             1370..1546
                     /locus_tag="VFU45_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357715.1"
                     /translation="MPVAAITAEWICTRCGTTNRRLVSEETRATADRCVNCHARHDVT
                     INDRPVRWDAKLAG"
     gene            1696..>2525
                     /gene="ruvB"
                     /locus_tag="VFU45_01255"
     CDS             1696..>2525
                     /gene="ruvB"
                     /locus_tag="VFU45_01255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU5357716.1"
                     /translation="MSRLQVTTPDALPEEAAADHALRPSRLTEFVGQEGVRQSLQIAI
                     EAAQKRGEPLDHLLFFGPPGLGKTTLAMLLAREMGVQLRTTSGPVLEKPGDLVGLLTT
                     LGRGDILFIDEIHRLRPQLEEFLYPAMEDWRVDVRIADGPNAQTIPMTVERFTLIGAT
                     TRFGLLTPPMRARFGIVERLSFYPDADLDTIVTRSAATLKVPIEPAGALEIARRSRGT
                     PRVANRLLRRVRDFAEVRAGGTITAQVATDALARLNVDEFGLDDMDARILNTIIEKFG
                     G"
BASE COUNT          367 a          935 c          872 g          351 t
ORIGIN      
        1 ggccgagatc ggcatggtcg ccaaggccac cgtccatgtc gacgggcccg acgcgctgaa
       61 ggtgctcaag ctcctcgacg cgctcgagga gctcgacgac gtcgccaagg tgttctccaa
      121 cttcgacatc gacgcctcca cgatggccga ggccgaggcc tgagggtcct gggcgtcgac
      181 ccgggcacgg ccatcgccgg attcggggtg gtggacggag gggcgggcgg gcgcgcccgc
      241 ccctcgctcg tcgaatgcgg cgtcatccgc acgacggccc gcgagacgct ccccgagcgt
      301 ctccgggtgg tgcacgaagg gatcgtcgag ctgatccagc gccaccgtcc cgatgtcgtg
      361 gcggtcgagg gaatcttcta cgctcgcaac gtccgctcga ccatcgtcct gggacacgcc
      421 cgcggggtca tcctccttgc cgccgcggcc gcggggttgc cggtcgccga gtacgcgccg
      481 gcgatggtca agcggacggt ggtgggccgg ggcgccgccg ccaaggcgca ggtcggctac
      541 atggtggccc agctcctccg gctcgcgcag gcaccgagcc ccgccgacgc ggccgatgcg
      601 gtggccatcg cactcaccca cctcctcagg ggcggtcgga tggtcggacc cgcgggtgac
      661 cgatgatcgc cgcgatctcg gggctcatct ccgcccgcga gggcgacacg gtcgtggtcc
      721 agacccaggg cggagtcggc tacgccatca ccctgcccct cggcgtgcac gaacgcctgc
      781 cgccggtggg ggaacgcgtg agcctcttca ccgagctggt ggtccgggag gacgggtggt
      841 cgctctacgg cttcgacgac ccgttcgagc gcaccgtctt ccagcgcctc ctgagcgcga
      901 gcggcgtcgg cccgaagctg gcgctggcgg tcctctccgc cctcggcccc gcgcgggcgg
      961 cccggagcat ccgcgaccgc gacctcgcgg cgctcgccag cgtcaccggg atcggcaaga
     1021 agaaggcgga gcgcatcgtg ctggaactgc aggaccggtt caaggagctt gctgacgccg
     1081 cgccgagcgc ggggcctgcc cttgcgggct cggccgccgt cgccgcgctc gccgcgctgg
     1141 ggtactccgc ggcgaatgcc gaggccgccg tgcgggccga gcttgccgcc ggcacgccgg
     1201 acgagaccgc ccagctggtg cggcgcgcgc tgcagcggct ggcggggaag tgatcgaagc
     1261 ttggagctga gagctgtgag ctttaagctg caggctgata gctgatagct gatagccgac
     1321 agccgatagc tcccggctcc tggcgctcag cccccacccc ggagccaaca tgcccgtcgc
     1381 cgccatcacc gcagaatgga tctgcacccg ctgcggcacc accaaccggc gccttgtgtc
     1441 cgaggagacc cgcgccaccg ccgaccggtg cgtcaactgc cacgcccggc acgacgtgac
     1501 gatcaacgac cgcccggtcc ggtgggacgc gaagctggcg gggtgacccg atagcgggtg
     1561 aaaagtgaaa agtgaggagt gaaaagggag aaaggggcag cggccacgct cggcgcccga
     1621 tccctgcccg ttacccttct cctccccagc ctccttctta cttctcactt ctcactcttc
     1681 acttacctta caggcatgtc ccgcctccag gtcacgaccc ccgacgcgct ccccgaagag
     1741 gccgcggcgg atcacgcgct ccgtccgtcc cggctgacgg agttcgtggg ccaggaaggc
     1801 gtgcgccagt cgctgcagat cgcgatcgag gcggcgcaga agcgcgggga gccgctcgac
     1861 cacctgctct tcttcggccc ccccggcctc ggcaagacca cgctcgcgat gctcctcgcg
     1921 cgcgagatgg gcgtccagct ccgcaccacc tcggggccgg tgctcgagaa gcccggcgac
     1981 ctcgtgggcc tgctcaccac gctcggccgc ggcgacatcc tgttcatcga cgagatccac
     2041 cggctccggc cgcagctcga ggagttcctc tacccggcca tggaggactg gcgggtggac
     2101 gtgcgcatcg ccgacgggcc caacgcgcag acgatcccga tgaccgtgga gcgcttcacg
     2161 ctcatcggcg ccacgacgcg cttcgggctc ctgaccccgc cgatgcgcgc gcggttcggc
     2221 atcgtcgagc ggctgagctt ctaccccgac gccgacctcg acacgatcgt gacccgctcg
     2281 gccgcgacgc tcaaggtgcc gatcgagccg gccggcgccc tcgagatcgc gcgccgcagc
     2341 cggggcacgc cgcgggtggc caaccggctg ctccgccggg tgcgggactt cgcggaggtc
     2401 cgggccggcg ggaccatcac cgcccaggtg gccaccgacg cgctcgcccg gctcaacgtc
     2461 gacgagttcg ggctcgacga catggacgcc cgcatcctca acacgatcat cgagaagttc
     2521 ggcgg
//
LOCUS       DASUAP010000052         6351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_451087, whole genome shotgun sequence.
ACCESSION   DASUAP010000052 DASUAP010000000
VERSION     DASUAP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451087"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1303
                     /locus_tag="VFU45_01260"
     CDS             158..1303
                     /locus_tag="VFU45_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357717.1"
                     /translation="MTRRSDERGIALILAVFALVVIGALVAGIFYTAQLEQRSGSNAL
                     AGQQAADAAQAGIDYAIANWDNSWTTMGTGNSITVSNKQLGSTGAYYTDLVTALNGFT
                     YLVTATGQSKNGSGSVLATRTYGMIYKKNIPTLNVQSSVTVAGGVSVGGSADINGNDV
                     PPTTGGWSSTCAGQPTSNQPAIRTDSSVSTHGSPTLTGGTVSHDTTVGTTITDVNQAY
                     NTFINSANVTIASGTITGANPVLNATTHACDRTNTSNWGDPEHSLAYTVGATTTYPCA
                     SYYPIIHVTGDLKVTGTIGQGVLLVDGNFTSGGGFTFYGLVIVKGSITKGNGTSNFYG
                     GLLSESASITDDLTGTINIQYSSCAINTVVSNSAVARPIVQRPFIQY"
     gene            1593..2636
                     /gene="pilM"
                     /locus_tag="VFU45_01265"
     CDS             1593..2636
                     /gene="pilM"
                     /locus_tag="VFU45_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEU5357718.1"
                     /translation="MGLFGRKKTTVGLDIGSGLIKVVEISHGGGDPVLSRVAFAPVSA
                     DAIVDGEIIDQGVVADAIKQLLASSKIKTKSVVIAVGGRDVIVKKIQMDRMSEPEARE
                     VIRWEAEQHVPFDMENVELDFQILDPEGEGLQMTVLLVAAKRELVEGRMSLLAEAGLE
                     ASIIDVDAFALHNAFELNHPDDLSGVVGLVNIGNEMSNINILEDGIPVLTRDLPVGVR
                     KFREDLQRERGLSVEEADKILQGFERNEMLDPFLESRGEELAVGIERAAAFLQSASRS
                     AGSLTRIFTVGGGARIPGLNKVLADRLRIPVEQGNPLARLQVADTAFETMSMDEVAPL
                     LMLPVGLALRSAA"
     gene            2665..3345
                     /locus_tag="VFU45_01270"
     CDS             2665..3345
                     /locus_tag="VFU45_01270"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEU5357719.1"
                     /translation="MITVNLRPGQKRKTGSAGKQLQARLKELAARVKEPLLIAAAAAW
                     VVVVAGLVWLYGSTSHQYSTLEPRLSQARSEEARFKGLMAQKRKAEQIRDSLLSQIRV
                     IRRVDGDRYIWPHLLDEVAKALPPYTWLVDLTIVGTQPVDTGAVDSTAHSKVQFQLDG
                     RTVDYQAYTQFLRQLEASPWISGVEPVNSQAVVEQGRPVTAFQIKATFTQADSAYIRT
                     VPLNQSVR"
     gene            3353..3964
                     /gene="pilO"
                     /locus_tag="VFU45_01275"
     CDS             3353..3964
                     /gene="pilO"
                     /locus_tag="VFU45_01275"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEU5357720.1"
                     /translation="MNQTRQVAILLIFLAAVAGYLGYDGFTGVLDGVKGKQERVRVMQ
                     DSVTALQKEIDSAKAVLAKGSIEQLRRRLEGYRQSLDLLRRLVPEENEVPNLLDQIST
                     RAKIRGVDLAGVKPGATINGPPPFRTNQYDIAVLGHYDQLGEFLSDIASLPRIIVPVG
                     FKVQAAPASAGQALGDSSGALLSAQFSIRTYVKTGKEAADSAQ"
     gene            3954..5027
                     /locus_tag="VFU45_01280"
     CDS             3954..5027
                     /locus_tag="VFU45_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357721.1"
                     /translation="MLNKRQLLAALAASVLVAGGCDRIKGWMGKGGIDQDSLTKAHAL
                     QARKDSLEKAQAARDSVARVHWIACSDSVEKALSKTAAGRRELKARAKMPAGMLLPSV
                     LKACGAEPPAPRPVAVAPAPGKPGAAAPPAGGKPGAPAPAPVTAAKPATAAPGAKPGA
                     VAPVGGAKPGAPTPAAQAKPATPSPAPAHPALTPAQARIARADSIRKAKDAAHADSLA
                     KAREKARVDSLAKAKADSIRQDSIALAKETEVVRETFAYTGGSRDPFVSLIQRNSSSV
                     EFGDLVLIGIYQDLRSSRNSVALLRNKNTGKRYKVHIGEKVGQRMTVSQITDHDVTFT
                     IQDFGFERQETLSLTKKGEEDNP"
     gene            5024..>6351
                     /locus_tag="VFU45_01285"
     CDS             5024..>6351
                     /locus_tag="VFU45_01285"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEU5357722.1"
                     /translation="MKPLRLTLLPAVLAVVAAPLVARPTGPRDGQVLSVAVQPAPGAA
                     EVVIGLRGAVEVNDFTLREPARLVLDLTGATLGVAPTLYDGVDRGGIKNIRVGQFAPN
                     VVRVVIDLSALKDYQVVRDGNSVRVHFGADRSFLAWSSLAPSAMPAPEAPARLAAARA
                     PEVSLPAPVRMDQQVPPDRPINVSYDNADIADVVAGFAAFSGRSIVLGAGIAGTIKVN
                     TEIKNKPWRAAFAAILSANGLSATETPEGIIRVDSPKALAALDSLEPLETRLVRVNYA
                     NASAIAKNLTGIISKRGSVVADSGTNSLIITDTRSKVSGVEDFVKGLDVRTPSVSIQA
                     KIILVNREDIEGLGIQYDLGAQAAGEGSFFNQLVQRIDPATGLPYQNPNINYVDLGGT
                     SMAAIGNAKATLTNPALNVILTTVIGGMSLTSFVQATQEINLTDVQAEPI"
BASE COUNT         1093 a         2264 c         2115 g          879 t
ORIGIN      
        1 aacgtaccca agatgcgcac catcaaggtg tcgctccgcg gcgtcaccag cggctcggtg
       61 tcggaggccg gcagcagctc ccgcgcgcag atcaacgaca gcctggtcac gtacgtgacg
      121 ctccgcaatg cgccgttcaa ctgaggccga ggtgcctatg acccgccgct ccgacgaacg
      181 agggatcgcc ctcatcctgg ccgtctttgc cctggtggtg atcggggcgc tcgtggccgg
      241 catcttctac accgcccagc tcgagcagcg gagcgggagc aacgccctcg ccggccagca
      301 ggccgccgac gccgcgcagg ccgggatcga ctatgccatc gccaactggg acaactcctg
      361 gaccacgatg ggcaccggca actcgatcac cgtttccaac aagcagctcg gctccaccgg
      421 ggcgtattac accgacctgg tcaccgcgct caacggcttc acctatctcg tcaccgccac
      481 cgggcagtcg aagaacgggt cggggagcgt gctggccacg cggacgtacg ggatgatcta
      541 caagaagaac atcccgaccc tgaacgtgca gtcgagcgtc acggtggccg gcggcgtgag
      601 cgtgggcggc agcgcggaca tcaacgggaa cgacgtgcca ccgacgacgg gaggctggtc
      661 gtccacctgc gccgggcagc cgacctccaa ccagcccgcc atccggaccg actcgagcgt
      721 ctcgacccac ggcagcccga cgctcactgg cggcaccgtg tcgcacgaca ccaccgtcgg
      781 caccacgatc accgacgtga accaggcgta caacaccttc atcaacagcg cgaacgtcac
      841 gatcgcctcc ggcaccatca ccggcgccaa tccggtgctg aacgccacga cgcacgcctg
      901 cgaccggacg aacacgagca actggggcga cccggaacac agcctggcct acacggtggg
      961 agccaccacc acgtacccgt gcgcgtccta ctatccgatc atccacgtga ccggcgacct
     1021 caaggtgacc ggcacgatcg ggcagggagt cctgctcgtg gatggcaatt tcacctcggg
     1081 gggcggcttc acgttctacg ggctcgtcat cgtgaagggg agcatcacca aggggaacgg
     1141 cacctccaac ttctacggcg ggctcctctc cgagagcgcc agcatcaccg acgacctgac
     1201 gggcacgatc aacatccagt actcgagctg cgccatcaac acggtggtct ccaactccgc
     1261 ggtggcccgg cccatcgtcc agcgaccgtt catccagtac tgacaccatc ccgccactgt
     1321 cacgccgggc cggcctctcg gggtcggccc ggcgtatttt tccggccttg aggccgccat
     1381 tcggcacccg aatggacgcc gaagacgcgg ccatggcgcg gcggcggagc cggaggttgg
     1441 cgggcacgtc atgaggtccg gcactggtaa agtctgaccc cgcaacatct tgttccgact
     1501 cacctcaagc accttcgagc ttgacaattt ccttgcccaa tggtagcgta gccagtccaa
     1561 gtccccattt ccacaaccac ggccgggtca gtatgggtct cttcggacgc aagaagacca
     1621 ctgtcgggct cgacatcggc agcgggttga tcaaggtggt cgagatcagc cacggcgggg
     1681 gtgacccggt gctgtcccgg gtcgcattcg cgccggtgtc ggcggacgcc atcgtcgacg
     1741 gtgagatcat cgaccagggc gtggtggccg atgccatcaa gcagctcctt gcctcctcca
     1801 agatcaagac caagtcggtc gtgatcgcgg tgggcgggcg cgacgtcatc gtcaagaaga
     1861 tccagatgga ccggatgtcg gagccggagg cccgtgaggt gatccgctgg gaggcggagc
     1921 agcacgtgcc gttcgacatg gagaacgtcg agctcgactt ccagatcctc gaccccgagg
     1981 gtgaggggct ccagatgacg gtgctgctgg tcgcggccaa gcgggagctg gtggaggggc
     2041 ggatgtcgct cctggccgag gcggggctcg aggcgagcat catcgacgtc gacgctttcg
     2101 cgctgcataa cgccttcgag ctcaaccacc ccgacgacct ctccggcgtg gtggggctgg
     2161 tcaacatcgg caacgagatg tcgaacatca acatcctcga ggacgggatc ccggtgctca
     2221 cgcgggacct cccggtcggg gtgcggaagt tccgggagga cctgcagcgc gagcgcggcc
     2281 tctcggtgga ggaggccgac aagatcctgc agggtttcga gcgcaacgag atgctcgacc
     2341 ccttcctcga gagccgcggc gaggagctgg cggtgggcat cgagcgggcg gcggccttcc
     2401 tgcagtcggc cagccggtcg gcggggtcgc tcacgcggat cttcaccgtc ggcggcggcg
     2461 cccggatccc ggggctcaac aaggtgctgg ccgaccggct ccgcatcccg gtggagcagg
     2521 gcaatccgct ggcccggctg caggtggccg acacggcctt cgagacgatg agcatggacg
     2581 aggtggcgcc gctgttgatg ctgccggtcg gactcgcgct caggagcgcg gcatgacgcc
     2641 gccccggagg atcaccaccc gatgatgatc acggtcaatc tccggcccgg gcagaagcgc
     2701 aagaccggga gtgccggcaa acagctccag gcccgcctga aggaactggc ggcccgggtc
     2761 aaggagcccc tgctcatcgc tgccgcggcg gcgtgggtcg tcgtggtggc ggggctcgtg
     2821 tggctctacg ggagcacctc gcaccagtac tccaccctcg agccgcgcct gtcgcaggcg
     2881 cgctcggagg aggcccgctt caaggggctg atggcccaga agcggaaggc cgaacagatc
     2941 cgcgactcgc tgctctcgca gatccgcgtc atccgccggg tcgacggcga ccggtacatc
     3001 tggccccatc tcctcgacga ggtggccaag gcgctgccgc cgtacacctg gctggtggac
     3061 ctgaccatcg tcggcaccca gccggtcgac accggcgccg tcgactccac cgcccacagc
     3121 aaggtccagt tccagctcga cgggcgcacg gtggactacc aggcgtacac ccagttcctc
     3181 cgccagctgg aggcgtcgcc gtggatctcc ggcgtcgagc cggtcaattc gcaggcggtg
     3241 gtcgagcagg ggcgcccggt cacggcgttc cagatcaagg ccacgttcac gcaggccgac
     3301 tcggcctaca tccgcaccgt accgctcaac cagtcggtga ggtgaccgcg cgatgaacca
     3361 gacccgacag gtcgcgatcc tcctcatctt cctggccgcc gtggccggct acctcgggta
     3421 cgacggcttc accggcgtgc tcgacggggt caaggggaag caggagcgcg tcagggtgat
     3481 gcaggacagc gtgaccgcgc tgcagaagga gatcgacagc gccaaggccg tcctggccaa
     3541 ggggagtatc gaacagctcc ggcgccggct cgaagggtac cgccagagcc tcgatctcct
     3601 ccgccggctg gtgccggaag agaacgaggt gccgaacctc ctcgaccaga tctcgacccg
     3661 tgcgaagatc cgcggcgtgg acctggcggg cgtcaagccc ggcgccacca tcaacgggcc
     3721 gccgcccttt cggaccaacc agtatgacat cgccgtgctg gggcactacg accagctggg
     3781 cgagttcctc tccgacatcg cctccctgcc gcgcatcatc gtgccggtcg ggttcaaggt
     3841 gcaggccgcg cccgcctcgg ccggccaggc cctgggcgac tcgagcggcg cgctcctgtc
     3901 ggcgcagttc tccatccgga cctacgtgaa gaccgggaag gaggcggccg acagtgctca
     3961 ataagcggca gctcctcgcg gcgctcgcgg cgtcggtcct ggtggccggc ggctgcgacc
     4021 gcatcaaggg atggatgggc aagggcggca tcgaccagga ctcgctcacc aaggcccacg
     4081 ccctccaggc ccggaaggac agcctggaga aggcgcaggc cgcccgcgac tccgtggccc
     4141 gggtgcactg gatcgcctgc agcgactcgg tcgagaaggc gctcagcaag acggccgcgg
     4201 gccggaggga actgaaggcc cgggccaaga tgccggcggg gatgctcctg ccctccgtgc
     4261 tcaaggcgtg cggcgccgaa ccgccggcac ccaggccggt ggccgtcgca cctgcgccgg
     4321 gcaagcccgg tgcggcggca cccccggccg gcggcaagcc gggcgctccg gcacctgctc
     4381 ccgtcaccgc ggccaagcct gcgacggccg cacccggggc caagcccggg gcggtcgcac
     4441 cggtgggcgg cgccaagccc ggagctccga cacccgcggc gcaggccaag cccgccacgc
     4501 cgtcccccgc gcccgcgcat ccggcgctga ccccggcaca ggcgcggatc gcccgcgccg
     4561 actcgatccg gaaggccaag gacgcggccc acgccgattc gctcgccaag gcgcgcgaaa
     4621 aggcccgggt ggactcgctc gccaaggcca aggccgactc gatccgccag gactcgatcg
     4681 cgctggccaa ggagaccgag gtggtgcggg agacgttcgc ctacaccggc gggagccgcg
     4741 acccgttcgt gtcgctcatc cagcgcaaca gctcgagcgt cgaattcggc gacctggtgc
     4801 tcatcggcat ctaccaggac ctgcgctcgt cgcggaacag cgtggcgctg ctccggaaca
     4861 agaacaccgg caagcgctac aaggtgcaca tcggcgagaa ggtcggccag cggatgaccg
     4921 tgtcgcagat caccgatcac gacgtgacgt tcaccatcca ggatttcgga ttcgagcggc
     4981 aggagacgct gtcgctgaca aagaagggtg aggaggacaa cccatgaagc ccctgcgtct
     5041 gaccctgctg ccggccgtgc tcgcggtcgt ggcggcccca ctggtggccc ggcccaccgg
     5101 cccccgcgac ggccaggtgc tgagcgtcgc cgtccagccg gcgcccggcg ccgccgaggt
     5161 ggtcatcggc ctgcgtggcg cggtcgaggt caacgacttc accctgcgcg agcccgcccg
     5221 cctggtgctc gacctcaccg gcgccacgct gggcgtggcc ccgacgctct acgacggcgt
     5281 cgaccgcggc gggatcaaga acatccgggt ggggcagttc gccccgaacg tggtgcgggt
     5341 ggtcatcgac ctgtcggcgc tcaaggacta ccaggtggtg cgcgacggga actcggtgcg
     5401 ggtccacttc ggcgccgacc gcagcttcct ggcgtggtcc tcgctggcgc cgtcggccat
     5461 gcccgcgccc gaggccccgg cccgcctggc cgccgcccgc gcgccggagg tgtcgctccc
     5521 ggccccggtg cggatggacc agcaggtccc gcccgaccgc ccgatcaacg tgagctacga
     5581 caacgccgac atcgccgacg tcgtggcggg ctttgccgcc ttcagcggcc ggtcgatcgt
     5641 gctcggcgcc ggcatcgccg gcaccatcaa ggtcaacacc gagatcaaga acaagccctg
     5701 gcgcgcggct ttcgccgcga tcctctcggc caacggcctc tcggccaccg agacccctga
     5761 ggggatcatc cgggtcgact cgcccaaggc gctcgcggcg ctcgattccc tggagcccct
     5821 cgagacccgg ctcgtgcgcg tcaactacgc caacgcctcc gcgatcgcca agaacctgac
     5881 cggcatcatc tccaagcgcg gatcggtggt ggccgactcg ggcaccaact cgctcatcat
     5941 caccgacacc cgaagcaagg tgagcggcgt cgaggacttc gtgaagggcc tcgacgtgcg
     6001 caccccgtcg gtgtcgatcc aggcgaagat catcctcgtc aaccgcgagg acatcgaggg
     6061 cctcggcatc cagtacgacc tcggcgccca ggcggcgggc gagggctcgt tcttcaacca
     6121 gctggtgcag cggatcgacc ccgccaccgg cctgccctac cagaacccga acatcaacta
     6181 cgtggacctg ggtggcacgt cgatggccgc catcggcaac gccaaggcga cgctcaccaa
     6241 cccggcgctc aacgtgatcc tcaccacggt gatcggcggg atgagcctga cctccttcgt
     6301 gcaggccacc caggagatca acctcacgga cgtgcaggcc gagccgatca t
//
LOCUS       DASUAP010000053         9368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1350312, whole genome shotgun sequence.
ACCESSION   DASUAP010000053 DASUAP010000000
VERSION     DASUAP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350312"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFU45_01290"
     CDS             <1..498
                     /locus_tag="VFU45_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357723.1"
                     /translation="DMRLNARVLGDGWVGGDPLPLQHRVALGGPDVLPGYDFRAVNCA
                     AGGYSDPAIPALCDRMLAAQVELRSRVHFGLLWHYRDKAHPELDRAFGLGEADLVVFT
                     DAGKAWLTGAGPGRVPNDRIPAFREWKADIGVGIDAGGIGAYLAKGFANGQPVQFSIR
                     LQQRF"
     gene            499..1149
                     /locus_tag="VFU45_01295"
     CDS             499..1149
                     /locus_tag="VFU45_01295"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEU5357724.1"
                     /translation="MRRTVWTVGLSLVLLAPAARAQDARVTLDVALTPDTAANGTRQA
                     ILRTDHLLDDPRIETMLSSEFPIRLHYRLELWRSRPSWFDAFERSVEWDVVIRHEPLL
                     DQYTVATVDVRTTRIDHYADRTALVGGLRRLTRIAIQPAERGEYYYAVALDISTLSDT
                     DIKELENFLQGDVGPAATGDEQVGSAISRGVRRILLQVAGLPNLRLEKQSSTFKVR"
     gene            complement(1136..2119)
                     /locus_tag="VFU45_01300"
     CDS             complement(1136..2119)
                     /locus_tag="VFU45_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU5357725.1"
                     /translation="MAGAARRQYGVLSPGRAGDFLLTTGLFNAIKAQDPDVDLTVVVG
                     PRAAGMARAHPAIDRVLVFDRHPLRILPFFLRLRDRVFDAWLDPKDHFSRNQTLIARA
                     VRARVKVGFNRPGGGPFQLLVRPPTDPTRHFSEMMMAPLDLLGVPWTRPPRLSIGVPE
                     AVEARAAETIGARAPFEVFVNISAGLPIRYWTEAKWIELLPRLAAERDTRFWLSAAPE
                     DREAAVRIEQAVAARGVAVRRTPAGSILDVAALVRRMDAVLTVDTSIVHLAAIYDRPI
                     VALYRDKRPECDQFRPLSTRQRVLFSSPGGLVAEIEVGDVAAAWRALLSGL"
     gene            complement(2122..2673)
                     /locus_tag="VFU45_01305"
     CDS             complement(2122..2673)
                     /locus_tag="VFU45_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5357726.1"
                     /translation="MRCLALNASFEPLTMVPVRRALRLVIEGKAEVVEADRHEVIRSE
                     RLTLPRPSIIRLVKFVHVPRRFRRQVTNTFLFARDNYSCQYCQRTQAQLRPRECLTRD
                     HLVPLSRGGGNEWTNVVTACSSCNTRKGNHLPEECGMHPHTVPHEPHFVHLAWAVRSL
                     TPIQSKYIRLFYGPEALRALGGR"
     gene            complement(2774..3223)
                     /gene="argR"
                     /locus_tag="VFU45_01310"
     CDS             complement(2774..3223)
                     /gene="argR"
                     /locus_tag="VFU45_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU5357727.1"
                     /translation="MKSRRHALILRVIAEERIENQDRLRSALARHGVDVTQATLSRDL
                     RELGLVKLSDPAGGAFYARPEESTPRPALDRLLPALLVSVDGVGPLLVLKTAAGAAGA
                     VTAALDQAGWDDVMGTIAGDDTILVVTRSERARLGVADRLKAFIRQH"
     gene            complement(3377..4594)
                     /locus_tag="VFU45_01315"
     CDS             complement(3377..4594)
                     /locus_tag="VFU45_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5357728.1"
                     /translation="MTGPVLRAGWVVPMAGPPIEGGEVVIDGDGRIGTVRPSELQPAP
                     PAGRWAILPGLVNAHTHLELTGFDGLVGDADFADWIPHVIRLKAARSAAGFLEAARRG
                     IRDSWAMGVTTVADCGDSGAVIQALHELGASGIAYHEVFGPDPADAPAQLAAWTARMR
                     ELRRFESVRVTLGASPHAPYTVSGELYRLAAAWARSEGMPLAVHIAEAADESALLRDG
                     TGGFAEAWRRRGVPIPEARCSPVEWLDRHGVFGPDTTAIHAVRVDAADVATLAQRGCG
                     VAHCPRSNRRHGHGDAPLQLILASIDRLGVGTDSVASVSPLDLMAEARAARALGGLSA
                     ERALELVTTGAARAIGLEREVGSLEAGKWGDAVAIRVPEDTTRDRIYEAVLASSPADV
                     AGTWVSGRLVHGA"
     gene            complement(4599..6767)
                     /locus_tag="VFU45_01320"
     CDS             complement(4599..6767)
                     /locus_tag="VFU45_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU5357729.1"
                     /translation="MPSRVIRQLLRWWHTPVIRRWTLTAAFSALGFGTALGWGSWARA
                     CLNNACPSVAGLGGYSADQASKVYAADGRLVTDFGLQRRTVISLSQMSPAVIAAFLAT
                     EDKRFYQHHGIDWIRVFGAIKDILIERRIAGGASTITMQLAGNLWPEEINRSDRSFRR
                     KLREAQVAMELERTYPKDKILELYLNQIDLGNRAFGVEAAAQRYFGKSARDLNVAEAA
                     TLAALPKSPAYYNPRRHRLHAIERRNTIINLLRDNGYLTAAEAERWKAYPLVLSNRSD
                     FSEVAPYFTEYVRQLMEARFGPDLYTSGYRIHTTLDLDIQLAAERSLEAQLEAIENGQ
                     YGPYHHETYAQYIQDKADKGDASQEGPSYSPYLQGISVVLEARTGNILAMVGGRDFDD
                     SKYNRAVNARRQPGSTFKPFVYSAALRAGHPLSEILVDEPLSVPMGPTEPPWEPQNYD
                     QTFRGPMTLRQALTMSDNIVTIRLGMEELGEQAVIAEATKFGITTKIPSVPSIHIGSA
                     DVQPLEMISAYTAFANMGERSVPNPILWVEDKDGNIVWRPQAQLKPVMDSDQAWLMID
                     VMRDVLRSPLGSAHQAWLDGNLDLPAAGKTGTTDDGTDVWFIGYTPDLVAGVWMGLDK
                     PDRIKNGAAGGILAAPAWMAMMKDIYQRRAVPRDPWPRPPDLTFAEIDRQSGERALPF
                     CPTENRFVESYLPGTEPKTYCHLHAFNPIGGGTPQYPGQN"
     gene            7078..8340
                     /gene="tyrS"
                     /locus_tag="VFU45_01325"
     CDS             7078..8340
                     /gene="tyrS"
                     /locus_tag="VFU45_01325"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU5357730.1"
                     /translation="MTALLPELTARGFVADLTPGLEERLARGPIIGYAGFDPTADSLH
                     VGNLVPVMGLAWLQRAGGTPIVLIGGGTGLIGDPSGKRAERPMLDAAQVVANARGIEA
                     QLRRFVSFEGKHGARVRNNLEWLAELRLIDFLREVGKHFTVSYMLQKESVKSRLETGI
                     SFAEFAYMLAQAHDFWHLYRHEGCELQVGGSDQWGNITAGCELVSRREGHQVHGLVFP
                     LLTTGSGAKFGKSDGDNIWLDPRRTSPYRFYQFWLNQEDADVERFLKLFTFLPLEEIA
                     AIGRTHRADPGKREGQRRLAAEVTALVHGRETADRAAAASEVLFGGQDVAATAADVLA
                     VLADELPVHRVTTAGVSGGMSLVDALVAGGLAATKAEARRGLEQRSYSVNGQKADANR
                     ALGGTDLLAGRYVLLQKGKKHHAMLVAD"
     gene            complement(8448..>9368)
                     /locus_tag="VFU45_01330"
     CDS             complement(8448..>9368)
                     /locus_tag="VFU45_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5357731.1"
                     /translation="ADEIALATNTTWGLALAAAALPLGAGDVVLASDKEFPANVYPWL
                     LQQERGVRLELVATTAQGWPDEAALLQRLERPEVKAVAVSLVQFSNGYRADLAALSAA
                     TRRLGKFLVVDGIQALGQVPVDLQATPVDILSCGGQKWLLSGWGSGFTYVRRELIGRL
                     RPAMTGWMAFEGTDDFSKLTAYDPRLRDDARRFELITLPFHDLAGFNTSVAMLLGVGI
                     DRIAAHLTALHAPVIAAAERTGARITSPRGAHGSGILCVAPKDLDGTFARLKAAGVYA
                     SLREGAIRLSPHFYTTPEEMERVAGILGGR"
BASE COUNT         1362 a         3222 c         3414 g         1370 t
ORIGIN      
        1 gacatgcgcc tcaacgcccg ggtgctgggg gatggctggg tgggcggcga tccgctgccg
       61 ctgcagcacc gggtggcgct gggcgggccg gacgtgctgc cggggtacga cttccgcgcc
      121 gtcaactgcg ccgccggcgg ctactccgac ccggcgatcc cggccctctg cgaccggatg
      181 ctggccgccc aggtcgagct ccggagccgg gtccacttcg ggctcctctg gcactaccgc
      241 gacaaggcgc accccgagct cgaccgcgcc ttcgggctcg gcgaagccga cctcgtggtg
      301 ttcaccgacg ccggcaaggc gtggctcacc ggcgccgggc cgggccgggt ccccaatgac
      361 cggatcccgg ccttccgcga atggaaggcg gacatcgggg tcggcatcga cgccggcggg
      421 atcggcgcct acctggccaa ggggttcgcc aacgggcagc cggtgcagtt ctccatccgg
      481 ctgcagcagc gcttctagat gcggcgcacc gtctggaccg tggggctgtc gctcgtcctg
      541 ctcgcgccgg ccgcgcgggc gcaggacgcg cgggtgacgc tcgacgtggc gctcaccccg
      601 gacaccgcgg cgaacggcac gcggcaggcg atcctgcgga cggaccacct gctcgacgac
      661 ccccggatcg agacgatgct gagctcggaa ttcccgatcc ggctgcacta ccggctcgag
      721 ctctggcggt cgcgcccgtc ctggttcgac gccttcgagc ggtcggtcga gtgggatgtg
      781 gtcatccggc acgagccgct gctcgaccag tatacggtgg ccaccgtcga cgtgcgcacc
      841 acgcggatcg accactacgc cgaccgcacg gcgctggtgg gggggctgcg gcgcctgaca
      901 cggatcgcca tccagcccgc cgaacgcggc gagtactatt acgcggtggc gctcgacatc
      961 tcgacgctct ccgacacgga catcaaggag ctggagaact tcctgcaggg cgatgtcggc
     1021 ccggccgcca cgggcgacga gcaggtcggc agcgccatct cgcggggagt ccgccggatc
     1081 ctgctgcagg tggcggggct gccgaacctc cgcctggaaa agcagtcgtc gaccttcaaa
     1141 gtccgctgag cagcgcgcgc cacgccgccg ccacgtcccc cacctcgatc tcggccacca
     1201 gcccaccggg cgaactgaac agcacacgct ggcgcgtact gagggggcgg aactggtcgc
     1261 actccgggcg cttgtcgcgg tagagcgcca cgatcgggcg gtcgtagatg gccgccaggt
     1321 gcacgatcga cgtgtccacc gtcagcaccg cgtccatccg ccgcaccagc gccgccacgt
     1381 cgaggatcga gcccgccggg gtccgccgca ccgccacgcc acgggccgcc accgcctgct
     1441 cgatccggac ggcagcctcg cggtcctccg gggcagcgct cagccagaac cgggtgtcgc
     1501 gctccgcggc gagccgcggc agcagctcga tccacttggc ctcggtccag tagcggatcg
     1561 ggagtcccgc cgagatgttc acgaacacct cgaacggcgc ccgggccccg atggtctcgg
     1621 cagcccgcgc ctccacggcc tcgggcacgc cgatcgacag gcgggggggc ctggtccacg
     1681 gtacgcccag caggtcgagc ggcgccatca tcatttcgct gaagtgccgc gtggggtcgg
     1741 tcggcgggcg caccagcagc tggaacggcc cgccccccgg ccggttgaag ccgaccttca
     1801 cccgcgcccg caccgcccgc gcgatcagcg tctggttccg gctgaagtgg tccttgggat
     1861 cgagccaggc gtcgaagacc cggtcgcgca ggcggaggaa gaacgggagg atccgcaggg
     1921 gatggcgatc gaacacgagc acccggtcga tggccggatg cgcgcgcgcc atgccggccg
     1981 cgcgggggcc cacgaccacc gtcaggtcga cgtcggggtc ctgggccttg atggcgttga
     2041 agagaccggt ggtgaggagg aaatcgcccg cccggccggg cgagaggacg ccgtactgcc
     2101 gccgggcggc accggccacg ctcagcgtcc gccgagcgcc cggagcgcct ccggcccgta
     2161 gaagagccgg atgtacttcg actggatggg cgtgaggctc cgcaccgccc aggcgaggtg
     2221 cacgaagtgc ggctcgtgcg gcacggtgtg cggatgcatc ccgcactcct ccggcaggtg
     2281 gttccccttg cgggtgttgc agctgctgca ggccgtcacg acgttggtcc attcgttccc
     2341 gccgccccgc gagaggggca ccaggtggtc gcgcgtcagg cactcacgcg gccgaagctg
     2401 cgcctgcgtc cgctgacagt actggcagct gtagttgtcg cgggcgaaca ggaaggtgtt
     2461 ggtgacctgg cggcggaagc gacgcggtac gtgcacgaac ttgaccaggc ggatgatcga
     2521 cggcctcggc agcgtcaggc gctcgctgcg aatgacctca tgccggtccg cctcgacgac
     2581 ctcagccttg ccttcgatga caaggcggag cgcccggcgg accggtacca tggtcagcgg
     2641 ctcgaacgac gcattcaacg cgaggcagcg caccccgatc ctcctggtgg actcaacccc
     2701 aagggcgata accacttgcc ttgtagcgta acggcgacac ttccggcggg caaggggaat
     2761 cgcgccgggc cggtcagtgc tgccggatga aggccttcag gcggtcggcc acgcccaacc
     2821 gggcgcgctc gctccgggtc acgaccagga tggtgtcgtc ccccgcgatc gtccccatta
     2881 catcgtccca tccggcctgg tcgagggccg ccgtcaccgc gccggcggcg ccggccgccg
     2941 tcttgagcac gagcagcggg cccaccccgt ccaccgacac gagcagcgcc gggagcagcc
     3001 tgtcgagggc cggccgaggc gtggactcct ccgggcgggc gtagaacgcg ccgccggcgg
     3061 gatccgacag tttgaccagc ccgagctcgc gcaggtcgcg cgagagggtc gcctgggtga
     3121 cgtcgacgcc gtgacgcgcc agcgccgagc ggaggcggtc ctggttctcg atccgctcct
     3181 cggcgatgac tcgaaggatg agcgcgtggc gtcgggattt catcgggtat cggggctcgg
     3241 cgctcagggt ggagggcttg gggcgtaggg cttagtgctt agtgcttagg gcttagcgct
     3301 tagggctcag ggctcagggc ttggcggacg tctcgcacga cccacaacaa acgactcacg
     3361 accaacgacc aacgactcac gccccatgca ccagacggcc ggagacccag gtgccggcga
     3421 cgtcggccgg tgaacttgcg aggacggctt cgtagatccg gtcgcgggtg gtgtcctccg
     3481 gcacccggat cgcgacggca tcgccccact tccccgcctc cagcgacccg acctcccgct
     3541 ccaggccgat ggcgcgggcg gcgccggtgg tcaccagttc gagcgcccgc tccgcgctga
     3601 gcccgccgag cgcacgggcc gcgcgggcct ccgccatgag gtcgaggggc gacacgctgg
     3661 cgaccgaatc ggtccccacc ccgagccgat cgatcgaggc caggatcagc tggagcggcg
     3721 catcgccgtg gccgtgccgg cggttcgacc ggggacaatg tgccacgccg caccctcgct
     3781 gcgcgagggt ggccacgtcc gcggcgtcca cccggacggc gtggatggcg gtggtgtcgg
     3841 gtccgaagac gccgtggcgg tcgagccatt ccaccggcga acaccgcgcc tcggggatcg
     3901 gcacgccgcg ccgccgccac gcctcggcga agccgccggt cccgtcccgg agcagcgccg
     3961 actcgtcagc ggcctcagcg atgtggacgg ccagcggcat tccttcactc cgggcccagg
     4021 ccgccgcgag tcggtagagt tcgccgctca cagtgtacgg ggcgtgtgga gacgcgccga
     4081 gggtgacgcg caccgactcg aagcggcgga gctcccgcat ccgggcggtc caggcggcga
     4141 gctgcgcagg ggcgtcggcg ggatcgggcc cgaagacctc gtggtacgcg atgccgctgg
     4201 cgccgagctc gtggagcgcc tggatgacgg cgccggagtc gccgcaatcg gccacggtgg
     4261 tgacgcccat cgcccacgag tcgcggatcc cccggcgtgc ggcctcgagg aacccggcag
     4321 ccgagcgggc cgccttgagc cggatgacgt gcgggatcca gtcagcgaag tcggcatcgc
     4381 cgaccagccc gtcgaagccg gtgagctcga ggtgggtgtg ggcgttgacc aggccgggga
     4441 ggatcgccca ccggccggcc ggaggggcgg gctggagctc cgacgggcgg accgtgccga
     4501 tccgaccgtc gccatcgatg acgacctccc cgccctcgat cggcggcccc gccatcggga
     4561 ccacccagcc ggcgcggagg acgggcccgg tcacgcgatc agttctggcc cgggtactgc
     4621 ggcgtgccgc cgccgatcgg attgaaggcg tgcaggtggc agtaggtctt gggctcggtg
     4681 cccggcaggt acgattcgac gaaccggttc tcggtggggc agaagggcag cgcccgttcc
     4741 cccgactgcc ggtcgatctc ggcgaaggtg aggtccggcg gccggggcca cggatcccgg
     4801 gggacggcgc ggcgctggta gatgtccttc atcatcgcca tccaggccgg ggccgccagg
     4861 atgccgccgg ccgcgccgtt cttgatgcgg tccggcttgt cgagccccat ccagacaccc
     4921 gccaccaggt cgggggtgta gccgatgaac cagacatcgg tgccgtcgtc ggtggtgccg
     4981 gtcttcccgg ccgccggcag gtcgagattg ccgtcgagcc aggcctggtg ggcgctcccc
     5041 agcgggctgc ggagcacgtc gcgcatcacg tcgatcatca gccacgcctg gtcggagtcc
     5101 atgaccggct tgagctgcgc ctggggccgc cagacgatat tgccgtcctt gtcctcgacc
     5161 cagaggatcg ggttcggcac gctgcgctcg cccatgttgg cgaacgcggt gtaggccgag
     5221 atcatctcga ggggctgcac gtcggcggag ccgatgtgga tcgaggggac gctggggatc
     5281 ttggtcgtga tgccgaactt ggtggcctcg gcgatgaccg cctgctcgcc cagctcctcc
     5341 atccccaggc ggatcgtcac gatgttgtcc gacatcgtga gtgcctggcg gagcgtcatc
     5401 gggccgcgga aggtctggtc gtagttctgc ggttcccacg gcggctcggt ggggcccatc
     5461 gggacgctga gcggttcgtc gaccaggatc tccgacaggg ggtgcccggc gcggagcgcg
     5521 gcggaataga cgaacggctt gaaggtggag ccgggctggc gccgggcgtt gaccgcgcgg
     5581 ttgtacttgc tgtcgtcgaa gtcgcggccg ccgaccatcg cgaggatgtt gccggtcctg
     5641 gcctcgagca ccaccgagat cccctgcagg tagggcgagt agctggggcc ctcctgggac
     5701 gcgtcaccct tgtccgcctt gtcctggatg tactgggcgt aggtctcgtg gtggtacggc
     5761 ccgtactggc cgttctcgat cgcctcgagc tgcgcctcga gggagcgctc cgccgcgagc
     5821 tggatgtcga ggtcgagcgt ggtgtggatc cggtacccgg aggtgtagag gtcggggccg
     5881 aagcgcgcct ccatcagctg gcgcacgtac tcggtgaagt acggcgccac ttcgctgaag
     5941 tcggagcggt tcgacagcac cagcggatac gccttccacc gctccgcctc ggcggcggtg
     6001 aggtagccgt tgtcgcgcag gaggttgatg atggtgttgc gccgctcgat ggcgtgcagc
     6061 cggtggcggc gggggttgta gtacgcgggg ctcttcggca gggccgcgag ggtggcggcc
     6121 tccgccacgt tgaggtcgcg ggcggacttg ccgaagtagc gctgggccgc cgcctcgacg
     6181 ccgaaggcgc ggttgccgag gtcgatctgg ttgaggtaca gctcgaggat cttgtccttg
     6241 gggtaggtgc gctcgagctc catggcgacc tgcgcctccc gcagcttgcg ccggaacgag
     6301 cggtcgctgc ggttgatctc ctcgggccag aggttgccgg cgagctgcat cgtgatcgtc
     6361 gacgccccgc cggcgatccg gcgctcgatc aggatgtcct tgatggcgcc gaacacccgg
     6421 atccagtcga tgccatgatg ctggtagaac cgcttgtcct cggtggccag gaacgcggcg
     6481 atgacggcgg gtgacatctg gctcagcgag atgacggtcc gacgctgcag cccgaagtcg
     6541 gtgaccagcc ggccgtcggc cgcgtagacc ttggacgcct ggtcggcact gtagccgccg
     6601 agccccgcga ccgaggggca ggcgttgttg aggcaggccc gcgcccacga gccccagccg
     6661 agcgcggtgc cgaacccgag cgccgagaac gccgctgtga gggtccagcg ccggatgacg
     6721 ggggtgtgcc accagcggag cagctggcgg atgacgcggg agggcatggt cgaatactag
     6781 ccgggggtgc gagcggggaa aagagagaag cgagggagaa ggaggagagg agaaggagaa
     6841 ggagaaggag aagaggaaaa ggagaaggaa gaggagaagg agacgcgagc cgcgagccgg
     6901 gagccgggag ccgggagccg ggagaaatga gactgcggcc gcagcggcgt gagatcaagc
     6961 aagtcgcgcc cggttccctg ttccccgttc cctgtcccct gttccctgtt cacttttccc
     7021 tcttccctct tcccttttca cttctcactt ctgacgccaa tccgatagat tccccgcatg
     7081 accgccctcc tgcccgagct caccgcccgc ggcttcgtag ccgacctgac tcccgggctc
     7141 gaggagcgcc tggcccgcgg cccgatcatc ggctatgccg ggttcgaccc gacggcggat
     7201 tcgctccacg tcggcaacct ggtgccggtg atgggcctgg cgtggctgca gcgcgcgggc
     7261 gggacgccga tcgtgctgat cggcgggggc acggggctga tcggcgaccc gagcggcaag
     7321 cgcgccgagc gcccgatgct cgatgcggcg caggtggtgg cgaatgcccg ggggatcgag
     7381 gcccagctcc ggcgcttcgt gtcgttcgag gggaagcatg gcgcgcgggt gcggaacaac
     7441 ctcgagtggc tcgccgaact gcggctgatc gacttcctgc gcgaggtcgg caagcacttc
     7501 accgtgagct acatgctcca gaaggagtcg gtcaagagcc ggctggagac ggggatctcc
     7561 ttcgccgagt tcgcgtacat gctggcgcag gcgcacgact tctggcacct ctaccggcac
     7621 gaggggtgtg agctgcaggt gggcgggagc gaccagtggg ggaacatcac cgccggctgt
     7681 gagctcgtct cgcgccggga ggggcaccag gtgcacgggc tcgtcttccc gctcctcacc
     7741 acgggatcgg gggccaagtt cgggaagagc gacggcgaca acatctggct cgatccccgg
     7801 cgcacgagcc cgtaccggtt ctaccagttc tggctcaacc aggaggacgc cgacgtcgag
     7861 cggttcctca agctcttcac gttcctgccg ctcgaagaga tcgctgcgat cgggcggacg
     7921 caccgcgccg accccgggaa gcgggagggg cagcggcgcc tggccgcgga agtgacggcg
     7981 ctggtgcacg gtcgggagac cgctgaccgg gcggccgccg cgagcgaggt cctcttcggc
     8041 ggccaggacg tcgcggcgac cgccgccgac gtgctggccg tcctggccga cgagctcccg
     8101 gtgcatcggg tcacgacggc gggtgtgtcg ggggggatgt cgctcgtgga tgcgctggtg
     8161 gcaggcgggc tcgccgccac gaaagccgag gcgcgccgcg gcctggagca gcgttcgtat
     8221 tcggtcaatg gccagaaggc tgacgccaac cgtgccctgg gcggcaccga cctcctggcc
     8281 ggtcgctacg tcctgctgca gaaggggaag aagcaccacg cgatgctcgt cgccgactag
     8341 ccgcgccgcg ctccccccat cgcccccctt ctcctccttc tccttctcct ccttctcctt
     8401 ctccttcttc tccttctccc tcttctcctc ttctcctctt ctcctcctca ccgccctccc
     8461 agtatccccg ccacccgctc catctcctcc ggcgtcgtgt agaagtgcgg cgacagccgg
     8521 atggcacctt cccggagcga cgcgtacacg cccgccgcct tgagtcgggc gaatgtgccg
     8581 tcgaggtcct tcggcgccac gcagaggatc cccgacccgt gcgccccgcg gggggaagtg
     8641 atccgcgcgc ccgtccgctc cgctgcggcg atgacgggcg cgtggagcgc cgtcaggtgg
     8701 gccgcgatcc ggtcgatccc cacgccgagc agcatcgcga ccgacgtgtt gaaccccgcc
     8761 aggtcgtgga acgggagggt gatgagctcg aaccggcgcg cgtcgtcccg gaggcgggga
     8821 tcgtaggcgg tgagtttcga gaagtcgtcg gtcccctcga acgccatcca tccggtcatc
     8881 gcgggccgga gccgaccgat cagctcacgc cggacgtagg tgaaccccga gccccagccg
     8941 gagaggagcc acttctgccc gccgcaggag aggatgtcga ccggcgtcgc ctgaaggtcg
     9001 accggcacct gccccagcgc ctggatcccg tcgaccacca ggaacttgcc aaggcgccgc
     9061 gtggcggcgg agagcgcggc caggtcggcg cggtagccgt tgctgaactg gaccagcgag
     9121 acggccacgg ccttcacctc ggggcgctcg agccgctgga ggagcgccgc ctcatcgggc
     9181 cacccctgcg cggtggtggc cacgagctcg aggcggacgc cccgctcctg ctggaggagc
     9241 caggggtaaa cgttggccgg gaattccttg tcgctcgcga ggaccacgtc gccggcgccg
     9301 agggggagcg ccgccgcggc gagtgccagc ccccaggtcg tgttcgtggc gagcgcgatc
     9361 tcgtcggc
//
LOCUS       DASUAP010000054         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_369105, whole genome shotgun sequence.
ACCESSION   DASUAP010000054 DASUAP010000000
VERSION     DASUAP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369105"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VFU45_01335"
     CDS             complement(<1..1242)
                     /locus_tag="VFU45_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5357732.1"
                     /translation="MTRPEPVSGRPGPVTALAMRGIRKAFGNVIALRNADLAVRSGEI
                     HALLGENGAGKSTLMHIAAGLVRPDAGAMALIGSEGFPATPRAAVERGVAMVHQHFTS
                     IAALSVSENIALATAWPVRPAPLRARVAALLKDLGLPLDPDARAGDLSVGLRQRLEIA
                     KALAAGPAVLLLDEPTGVLSPAESAELFRSLRTFVAAGGAAVLITHRLDEALEQADAI
                     TVLRQGEVTLADTPAATLTRDRLLTAMLGPEPVVAPVRAHRPPDASVAVAVRGLAVER
                     EGRGGMAVRDVSLEVRPGEIVVVAAVEGNGQRELLRAIAGALPVAGGTRRVASPVAFI
                     PEDRTTEALIGEFSLVENLALGDRAAPRRGWLDWTEVRGRAARLIAEFDVKAQGPDAP
                     AASLSGGNQQKFVNARALDVRP"
     gene            complement(1277..2035)
                     /locus_tag="VFU45_01340"
     CDS             complement(1277..2035)
                     /locus_tag="VFU45_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU5357733.1"
                     /translation="MTRAAELRDLVAWLDGYLRIAEAPDHPNAVNGLQVENSGQVTRI
                     VAAVDASLETIAGLRRNDLLLTHHGLLWDGNQPVTGRRYRRLKALFDLDAALYSAHIP
                     LDLHPDVGNNVLLARALGVGVSGGFGLHQGVAIGVQGALAMDRSDLVARIASLLGGPL
                     KLIAGGPTRTSRIAIVTGAGGGLVREARDAGCDTLITGEGAHHTYFDAVEWGVNLVYA
                     GHYATETLGVKALAEKTALQFGLPWEFHDHPTGL"
BASE COUNT          290 a          787 c          781 g          281 t
ORIGIN      
        1 gggacgcacg tcgagcgcac gcgcgttcac gaacttctgc tggttaccgc cgctcaggct
       61 cgcggccggc gcatccggcc cctgcgcctt gacgtcgaat tccgcaatga gccgcgccgc
      121 gcggccacgc acctcggtcc agtcgagcca gccccggcgc ggcgcggccc ggtcgcccag
      181 cgccaggttc tcgaccaggg agaactcgcc gatcagcgcc tcggtggtgc ggtcctccgg
      241 gatgaacgcc accggggacg ccacccgccg cgtgcccccc gcgaccggca gcgcgccggc
      301 gatggcccgc agcagctccc gctgcccgtt cccctcgacg gcggcgacca ccacgatctc
      361 tcccggccgc acctcgagcg acacatcgcg gacggccatc cccccgcgcc cttctcgctc
      421 cacggcgagt ccgcgcacgg cgacggccac cgaggcgtcg ggcgggcggt gcgcgcgcac
      481 cggggcgacg acgggctcgg ggcccagcat cgcggtgagg aggcggtcgc gggtcagggt
      541 tgcggccggc gtgtccgcga gcgtgacctc cccctggcgg agcaccgtga tcgcgtccgc
      601 ctgttccagc gcttcgtcga gccggtgggt gatgagcacc gccgccccgc ccgcggccac
      661 gaacgtccgc agcgagcgga agagctccgc cgactccgcg ggcgacagca ctccggtcgg
      721 ctcgtcgagc aggagcacgg ccggccccgc cgcgagtgcc ttggcgatct ccaggcgctg
      781 ccggagtccg accgacaggt cgcccgcccg cgcatcggga tcgagcggca accccaggtc
      841 cttgagcaac gccgccacac gggcgcggag cggggcgggg cgcaccggcc atgcggtggc
      901 cagcgcgatg ttctccgaca ccgacaacgc cgcgatcgag gtgaaatgct ggtgcaccat
      961 cgccaccccg cgctcgacag cggcgcgggg cgtggcgggg aagccctcgc tcccgatgag
     1021 cgccatggcg ccggcatcgg gacggaccag gccggctgcg atgtgcatca gggtcgactt
     1081 ccccgccccg ttctcgccga ggagggcgtg gatctcgccg gatcggaccg ccaggtccgc
     1141 attgcggagt gcgatgacgt tgccgaacgc cttccggatc ccccgcatcg ccagcgcggt
     1201 gacgggcccg gggcgaccgg aaacaggttc gggacgggtc acggtgggaa gcgcgccggc
     1261 ggggtacccc ggcgcgtcaa agcccggtgg ggtggtcgtg gaactcccac ggcaggccga
     1321 actggagggc cgtcttctcg gccagcgcct tgacgcccag cgtctcggtg gcgtagtggc
     1381 cggcgtagac caggttgacg ccccactcca ccgcatcgaa gtacgtgtgg tgtgcgcctt
     1441 ctccggtgat gagtgtgtcg catccggcgt cgcgcgcctc gcgcaccagg cctccgccgg
     1501 cgccggtgac gatggcgatc cgtgacgtcc tggtcggtcc gccggcgatg agtttcagcg
     1561 gcccaccgag cagcgaggcg atgcgcgcca ccagatcgct gcggtccatg gccagcgcgc
     1621 cctggactcc gatggccacg ccctggtgca gcccgaaccc gccactcacc ccgacaccga
     1681 gcgcccgggc aagcaggacg ttgttgccca cgtcggggtg aagatcgagc gggatgtggg
     1741 ccgagtagag cgcggcgtcg aggtcgaaga gcgccttgag gcggcggtag cggcgaccgg
     1801 tgaccggctg gttcccgtcc cagagcagtc cgtggtgggt caggagaagg tcgttgcggc
     1861 gcagcccggc gatcgtctcg agactcgcgt cgaccgcggc gacgatgcgg gtcacctgcc
     1921 cgctgttctc gacctggagc ccgttcaccg cgttcgggtg gtcgggtgcc tcggcgatcc
     1981 ggagatagcc gtcgagccag gcgaccaggt cccgcagctc tgcggcgcgc gtcattgctg
     2041 gtccggcacc gggatcagcg tcccggcgga gatcgagtcc gccgcggcgg ccacccgacg
     2101 agagagttcg gccggcaccc gtgccgtgag cgtggtatc
//
LOCUS       DASUAP010000055         7796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_178397, whole genome shotgun sequence.
ACCESSION   DASUAP010000055 DASUAP010000000
VERSION     DASUAP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178397"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1905)
                     /locus_tag="VFU45_01345"
     CDS             complement(<1..1905)
                     /locus_tag="VFU45_01345"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5357734.1"
                     /translation="MPGARPTITPMPLVARRAAATLMLLAAATVSAAPATLAAQGAQS
                     ATSSIQQAAAAPLPASARSVTATRATVPPVIDGRDDDAVWRTAPAITGFRVYRPVEDG
                     APKFRTEAKVAYDASNLYVFIRAYDPHPDSIVGLLARRDAMTPSDLLSVFIDGYHDRR
                     TGNEFDVNPAGVKLDAAIYQDGNEDMAWDGIWDVATRIDSLGWTAEYRIPLSQIRHAA
                     EPSDTFGFTVARQVERTSEQSSWPLYSVSKPGLASQLGTLTALNDLAPPRRIELAPYL
                     VTKNIEVPSGTGFGRSQRLTAGADLKVAVASNLTLTGTINPDFGQVEADPAVLNLSAF
                     EPFFEEKRPFFVEGNGLFQFSVDCSAVNCSNEQLFYSRRIGRAPHLTDQYGDANSPTA
                     TPIDGAAKLTGQLPGGLSLGVIDAYTPRETGVAGATIEPATNYAVLRATKDFRGGASG
                     VGLMFTGVDRDQDSWTAPYLAHSAYVGALDFRHQFLNRRYELSGSFDLTTVNGSTAAI
                     TALQEDPVHYYQRPDGPLSVDSTRTSLSGDAEEIKFGKIAGVRTQWETSYLRRSPGFE
                     VNDLGYLRQADQQSWNNWLGLSWNEQTRAYLRFRWNFNWWQWWSAAGLPTERAFNTNA
                     HAQLLNRWFVN"
     gene            complement(2136..2921)
                     /locus_tag="VFU45_01350"
     CDS             complement(2136..2921)
                     /locus_tag="VFU45_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5357735.1"
                     /translation="MTIRLSRLLAWTVLLLAPAAALAQAPAEHSAPADLLALDAGADS
                     ALVGSRIRQVSQALMTGVASGDTALWDRYLADDAMLTDENGDTHTKREVLASLRPLPP
                     GFSGQIHVEHAIPHVRGDLATLTYDAMEDEVVFGQHIGTRYRTTDIYAWRHGRWRLFG
                     SHTSVIPSEHSAVPGDTVRYGDYVGEYAVSQTMRLVVTSTAAHLYAGSPGHPPAELFP
                     LGPDRFFRKGAPRGERLFVRDPAGRVVAMIDRRDNNDLVWRRH"
     gene            3043..3951
                     /locus_tag="VFU45_01355"
     CDS             3043..3951
                     /locus_tag="VFU45_01355"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEU5357736.1"
                     /translation="MRTMAGRGAVVALVILLAPGTLCAQGGDGTFHLRSGGLERSYLL
                     HAPAGSGARPLVVLLHGRLGTGAGMAKMTHFDAVADRAGILVAYPDGWRRSWNDGRPG
                     TPAHRRQVDDVSFILAMIDDIGRRMPVDPRRVYVAGMSNGGFMTERLACEAGERFAAV
                     AVDVATLSDSLASTCRLPRPVPAIFFHGTADPLVPYGGGRMGDRGSGLSVDETVATWA
                     RWNGCAPSPVMESLPDTAREGIQVTRRTWPRCRDGADVVAYKFDGGGHAWPGGTQYLP
                     ARFVGRVSRNVDASEEIGRFFERHIR"
     gene            4055..5167
                     /locus_tag="VFU45_01360"
     CDS             4055..5167
                     /locus_tag="VFU45_01360"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5357737.1"
                     /translation="MSKKLRILIGVVFIVGGLIWWLTRTPKYLTLTGIVTTNDVVVSP
                     LVSGQVGQFLVVEGDSVKPGQLLATIVPDELRADRNYYLHSAQGFAAEAAASEATLRF
                     QVQQAASQLREAEANLAATKAQQAEAQATVDHAKRNWDRAEALAKAGQLAPQDADQSR
                     TTWVVAEARLDAADKQVTAGEAAVALARAQADQVTATRQSALSARQQEAAASAQATKA
                     DVRLGYTELRAPIGGFVDVRAARTGEVVNAGQPVVALIDPDDLWIRADVEETYADRVR
                     PGDSLSVRLPSGDVRRGVVFYRGVDAGFATQRDVSRTKRDIKTFEIRIRLDNRDRRLA
                     VGMTAYVLFPTARPAERGAPPAPPTPDDRPTPPASR"
     gene            5164..6153
                     /locus_tag="VFU45_01365"
     CDS             5164..6153
                     /locus_tag="VFU45_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5357738.1"
                     /translation="MTVAIDVRNIVKRFGDFTAVAGISFGVQDGEIFGLLGPNGAGKS
                     TLIRMMTTLVPPTEGTAIIGGFDVAQDPDGVRNVIGVIPQAMTSDIDLTVEENIRIFA
                     RLYGVPREKRIRLTRELLEAVELTQWADKPVKNLSGGMRRRVEIARGLIHEPKIFFLD
                     EPTTGLDPVSRTAVWEMLRKVKQERDLTVLITTHYMDEADKLCDRIAIVDHGKLVALD
                     SPLRLKAAIGGTNAVEVAFDHAPDGWKDRLGAVPAVEAVTQDGHVFRLHSKDGPATTL
                     GLLDLAHEAGVEVRSLSVQSATLDDVFVHFTGRQLRDQLQEASAADRLAPFRR"
     gene            6150..7280
                     /locus_tag="VFU45_01370"
     CDS             6150..7280
                     /locus_tag="VFU45_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5357739.1"
                     /translation="MKRVVVGRAAAIIERELIRFKRSRALLIMSLTAPLVQLIVLGYA
                     FGGAVKHLRLGVVDQDGGLPAVKLRELAGAVSANAQTFTQSSYADMGQALTDLKNGHL
                     NGVLAIPPGFSRKVLAGQGPRVALVEDNSDNFVAASLTGSLSGVVQAYDQPPETSDRM
                     AGATQLEQVEVYPYVPYIQYLLPGSIVMSIFMMVMIGGGIIYIDDKARGLHEGYLVTP
                     ISKFELIAGFTVSGAIKAILAGMVLMTVGSLIAGVPNPFDPLRMVRLFVVICFTSVAL
                     VAMMFLLMARMSDPLLPRALFGVLNTILYFPSGAVYPQQAFPGWMRAIAVADPFTYAV
                     HAFKALLLKNTGFAAIGTDLLYLGLFAAVTMTGATLLFRRSL"
     gene            7277..>7796
                     /locus_tag="VFU45_01375"
     CDS             7277..>7796
                     /locus_tag="VFU45_01375"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HEU5357740.1"
                     /translation="MRGPDGRGSSEDTRRRLFDAARELFTDQGFDNVTVRDLCTRAGA
                     NLAAINYHFGDKLALYLEVVRSAVEEMRSTNHEMMAGPELPAADRLRRYVREYVPRLM
                     LIDRRRAWIQKLMRNEMSEPTAAAQLIIEEILRPRVAYLTGIVAELVGCAPDDPRVTR
                     CVSAIQMQCFACI"
BASE COUNT         1163 a         2633 c         2801 g         1199 t
ORIGIN      
        1 gttcacgaac cagcggttga ggagctgggc gtgcgcgttg gtgttgaacg cccgctcggt
       61 cgggagcccc gccgccgacc accactgcca ccagttgaag ttccagcgga agcggaggta
      121 cgcccgggtc tgctcgttcc acgagagccc gagccagttg ttccagctct gctggtcggc
      181 ctggcgcagg tagccgaggt cgttgacctc gaatccggga gagcgccgca ggtagctcgt
      241 ctcccactgg gtgcggaccc cggcgatctt cccgaacttg atctcctcgg cgtcgcccga
      301 cagcgaagtc cgggtcgagt cgacgctgag cgggccgtcg gggcgctggt agtagtggac
      361 cgggtcttcc tgcagcgcgg tgatggcggc ggtcgagccg ttcaccgtcg tgaggtcgaa
      421 cgaccccgac agctcgtagc gccggttcag gaactggtgc cggaagtcga gggcgccgac
      481 gtaggcggaa tgcgccaggt acggggctgt ccaggaatcc tggtcgcggt cgaccccggt
      541 gaacatcagg ccgacgccgc tggcaccgcc gcggaagtcc ttggtggcgc gcaggaccgc
      601 atagttggtg gcgggctcga tggtggcgcc ggccacgccc gtctcccgcg gggtataggc
      661 atcgatgacg ccgagcgaga gccctccggg gagctggccg gtcagcttgg ccgcgccgtc
      721 gatcggggtg gcggtggggg agttcgcgtc gccgtactgg tcggtcaggt gcggcgcccg
      781 gccgatccgg cgggagtaga agagctgctc gttgctgcag ttgacggcgc tgcagtcgac
      841 gctgaactgg aagaggccgt tcccttcgac gaagaagggg cgcttttcct cgaagaacgg
      901 ctcgaacgcc gagaggttga gcaccgccgg atcggcctcg acctgcccga aatcgggatt
      961 gatcgtgccg gtgagtgtca ggttggacgc caccgccacc ttgaggtcgg cgccggcggt
     1021 caggcgctgg gaccgtccga atccggtgcc cgacgggacc tcgatgttct tggtgacgag
     1081 atacggtgcc agctcgatgc ggcggggcgg ggccaggtcg ttgagcgccg tcagtgtgcc
     1141 cagctgtgac gcgagccccg gcttggacac gctgtagagc ggccagctcg attgctcgga
     1201 ggtacgctcc acctgccgcg ccacggtgaa accgaacgta tcgctcggct cggcggcgtg
     1261 ccggatctgg gacaacggga tgcggtactc cgctgtccac cccagcgaat cgatccgggt
     1321 ggccacgtcc cagatcccgt cccaggccat gtcctcgttc ccgtcctggt agatggccgc
     1381 atcaagcttg acgccggcgg ggttcacgtc gaactcgtta cccgtgcgcc ggtcgtgata
     1441 gccatcgatg aacaccgaca ggaggtcgga aggcgtcatt gcgtcgcgtc gggccagcag
     1501 gcccacgatg ctgtcggggt ggggatcgta cgcccggatg aacacgtaga ggttgctggc
     1561 gtcgtaggcg accttggcct cggtgcggaa cttgggggcg ccgtcctcca ccggccggta
     1621 gacccggaat ccggtgatgg ccggtgccgt gcgccacacg gcgtcgtcat cccggccgtc
     1681 gatgaccggc ggcaccgtgg cccgggtcgc ggtcaccgac cgggccgagg ccgggagggg
     1741 tgccgcggcg gcctgctgga tcgacgacgt tgcggactgc gccccctgcg cggcgagggt
     1801 cgccggggcc gctgataccg tcgcggccgc caggagcatc agggtcgcgg ccgctcggcg
     1861 ggcaaccaag ggcatcggag tgatggtggg gcgtgcgccg ggcattcccg ctccggaagt
     1921 gaaatgctga aatttcagca tattttatct gccgttgcgg actgcgtcaa gtcgcggcgc
     1981 cagtgccggc atcgccttgt gagactggtg caccgcatta cgcgcctcgt ccggggatgc
     2041 ggattagacg agacgcgggg cccaatggtt gcacgttgca cgattggaac ggggtgggga
     2101 ggagatcgcc gtccccggac ctgccactgc cggcgtcagt gacgccgcca gacgaggtcg
     2161 ttgttgtccc gtcggtcgat catggcgacc acccgtccgg ctgggtcccg cacgaagagc
     2221 cgctctcccc ttggcgcccc cttccggaag aaccgatcgg ggcccagggg gaagagctcc
     2281 gcgggcgggt ggcctgggct gccggcatac aggtgggccg cagtggaggt cacgacgagc
     2341 cgcatcgtct gggaaacagc gtattcgccc acgtagtccc cataccggac agtgtcgcct
     2401 ggaacggccg agtgttcgct cgggatgacc gatgtgtggg aaccgaagag ccgccagcgg
     2461 ccgtggcgcc acgcgtagat gtcggtggta cggtatcggg tgccgatgtg ctggccgaag
     2521 acgacttcgt cctccatcgc gtcgtaggtc agcgtggcga ggtcgccgcg gacgtgcggg
     2581 atggcgtgct ccacgtggat ctgcccggag aagccgggcg gaagcgggcg cagcgaggcg
     2641 aggacctccc gcttcgtgtg tgtgtcgccg ttctcgtcgg tgagcatcgc gtcatcggcg
     2701 aggtagcggt cccagagcgc tgtatcgccg ctcgcgaccc cggtcatcaa cgcctggctc
     2761 acctggcgga tacgtgagcc gaccagcgct gagtcggcac ccgcatctag tgccagcagg
     2821 tcggcggggg ccgagtgctc ggccggcgcc tgcgccagtg ccgcggcggg agccaggagg
     2881 aggacggtcc aggcgaggag gcgagacagg cggatggtca ccgcggactc cggtggatgg
     2941 cttgcgatcg ggagccgtgg tcggcaccct tgtcccgaga gggaacacct ggcacatgcg
     3001 cgtcccgatc cacgacgatg ccgagaggga atgggtttcc gaatgaggac aatggccggc
     3061 cgcggggcgg tggtcgccct ggtgatcctg ttggcgccgg gcacgctctg cgcgcagggg
     3121 ggagacggca cgttccacct ccggagcggc ggcctcgagc gcagctactt gctccacgcg
     3181 cctgccgggt ccggcgcccg cccgctcgtg gtgctcctcc atggccggct cggcaccggc
     3241 gcggggatgg ccaagatgac ccacttcgac gccgtcgccg accgggcggg gatcctcgtg
     3301 gcgtatcccg atggatggcg ccggagctgg aatgatgggc gcccgggcac gccggctcat
     3361 cggcgccagg tcgacgacgt gtcattcatc ctcgcgatga tcgacgacat cgggcgccgg
     3421 atgccggtgg acccgcgccg ggtgtacgtc gcggggatgt cgaacggcgg gttcatgacc
     3481 gagcggctcg cgtgcgaggc cggcgagagg ttcgccgccg tcgccgtcga tgtcgcgacc
     3541 ctgagcgatt cgctggcgag cacctgccgg cttccgcgcc ccgttcccgc gatcttcttc
     3601 cacggcaccg ccgatccgct cgtgccttac ggcggcggcc ggatgggcga tcggggctcc
     3661 ggcctgagcg tggatgagac cgtggcgacg tgggcccgct ggaacgggtg cgcaccatcg
     3721 cccgtgatgg aatcgctgcc cgacacggcg cgcgagggga tccaggtcac ccggcgcacc
     3781 tggccgaggt gccgggacgg ggcggacgtg gtggcgtaca agttcgatgg gggcgggcac
     3841 gcgtggccgg gggggacgca gtacctgccg gccaggttcg tggggcgggt gtcgcggaac
     3901 gtcgatgcca gcgaggagat cgggcggttc ttcgaacgac acatcagata gcctgcacct
     3961 cgtgcggtga gaactcgact tgcaccaatg aaacaactgt tttatacatc cgtatgtcag
     4021 ccccagcccc cgcagtcggc tcgtggagat acggatgtcg aagaagctcc ggatactgat
     4081 cggcgtcgta ttcatcgtgg gtggcctcat ctggtggctc acccgcaccc ccaagtacct
     4141 gaccctcacc gggatcgtca ccaccaatga cgtggtggtg agccccctcg tctcgggcca
     4201 ggtcgggcag ttcctcgtgg tcgagggaga ctcggtcaag ccgggccagc tcctcgccac
     4261 gatcgtcccc gacgaactcc gcgctgaccg caactactac ctccactccg cccagggctt
     4321 cgccgccgag gcggcggcga gtgaggcgac cctccgcttc caggtacagc aggccgcctc
     4381 ccagctccgc gaggcggagg ccaatctcgc tgccacgaag gcccagcagg ccgaggcgca
     4441 ggcgaccgta gaccacgcga agcggaactg ggatcgggcc gaagcgctcg ccaaggccgg
     4501 gcagctggca ccccaggacg ccgaccagag ccggaccacg tgggtcgtgg cagaggcccg
     4561 gctcgatgct gccgacaagc aggtgacggc gggcgaggcg gccgtggcgc tcgcccgcgc
     4621 gcaggcggac caggtgaccg cgacgcgcca gtccgcgctg agcgcccgcc agcaggaggc
     4681 ggccgcctcc gcccaggcca ccaaggccga cgtccgcctc ggctacaccg agctccgcgc
     4741 cccgatcggc ggcttcgtgg acgtgcgggc ggcgcgcacc ggcgaggtgg tgaacgccgg
     4801 ccagcccgtc gtcgccctca tcgaccccga cgacctctgg atccgcgccg acgtcgagga
     4861 gacgtacgcc gaccgggtgc ggccggggga ttcgctcagc gtgcgccttc cgtccggcga
     4921 cgtccggcgc ggcgtcgtct tctatcgcgg cgtggacgcc ggcttcgcga cccagcgcga
     4981 cgtgagccgc accaagcggg acatcaagac cttcgagatc cggatccggc tcgacaaccg
     5041 cgaccggcgc ctggcggtcg ggatgacggc gtacgtcctc ttcccgaccg cgaggcccgc
     5101 agagcgtggg gcgccgccgg cgccccccac gccggacgat cgacccactc cgcccgcgtc
     5161 ccgatgaccg tcgccatcga cgtccggaac atcgtcaagc gcttcggcga cttcaccgcc
     5221 gtggcgggga tctccttcgg cgtccaggac ggcgagatct tcggcctcct cgggcccaac
     5281 ggcgccggga agtccacgct catccggatg atgaccacgc tggtcccgcc caccgagggc
     5341 acggcgatca tcggcggctt cgacgtggcg caggaccccg acggcgtgcg gaacgtgatc
     5401 ggcgtcatcc cgcaggcgat gacgagcgac atcgacctca ccgtcgagga gaacatccgg
     5461 atcttcgcgc ggctctacgg cgtcccgagg gagaagcgga tccgcctcac ccgggagctc
     5521 ctcgaggcgg tggagctgac ccagtgggcc gacaagccgg tcaagaacct gtcgggcggg
     5581 atgcggcggc gggtcgagat cgcccgcggg ctcatccacg agccgaagat cttctttctc
     5641 gacgagccga ccacggggct cgacccggtg tcgcgcacgg cggtctggga gatgctccgg
     5701 aaggtcaagc aggaacggga cctcacggtg ctcatcacca cccactacat ggacgaggcc
     5761 gacaagctct gcgaccgcat cgcgatcgtg gaccacggga agctcgtcgc cctcgactcc
     5821 cccctccggc tcaaggcggc gatcggcggg accaacgcgg tcgaggtcgc gttcgaccac
     5881 gcccccgacg ggtggaagga ccgcctcggc gcggtgccgg cggtcgaggc ggtgacgcag
     5941 gacgggcacg tcttccggct ccattcgaag gacggccccg ccaccaccct tggcctcctc
     6001 gacctcgccc acgaagcggg ggtcgaggtg cggtcgctct cggtgcagag cgcgacgctc
     6061 gacgacgtct tcgtccactt cacggggcgc cagctccgcg accagctgca ggaggcgtcg
     6121 gcggcggacc gcctggcgcc gttccggcga tgaaacgcgt cgtggtcggg cgcgccgccg
     6181 ccatcatcga gcgggagctg atccgcttca agcggagcag ggcgctgctc atcatgtcgc
     6241 tcacggcgcc gctggtgcag ctcatcgtcc ttggctacgc cttcggcggc gcggtcaagc
     6301 acctccggct cggcgtggtg gaccaggacg gcgggcttcc ggcggtcaag ctccgcgagc
     6361 tggcgggggc cgtctcggcc aacgcccaga cgttcacgca gtcctcctac gccgacatgg
     6421 ggcaggcgct caccgacctc aagaacggcc acctcaacgg ggtgctcgcg atcccgcccg
     6481 gattctcccg caaggtgctg gcgggccagg ggccgagggt ggccctcgtc gaggacaatt
     6541 cggacaactt cgtcgcggcg agcctgaccg gaagtctctc cggcgtggtg caggcgtacg
     6601 accagccccc cgagacgtcg gaccggatgg ccggcgccac ccagctcgag caggtcgagg
     6661 tgtacccgta cgtgccgtac atccagtacc tgctcccggg ctcgatcgtg atgtccatct
     6721 tcatgatggt gatgatcggc ggcgggatca tctacatcga tgacaaggcg cgcggcctgc
     6781 acgaggggta cctggtgacg ccgatcagca agttcgagct gatcgccggg ttcaccgtct
     6841 cgggggccat caaggccatc ctggccggga tggtgctgat gacggtcggc tcgctcatcg
     6901 cgggggtgcc gaacccgttc gacccgctcc ggatggtgcg gctcttcgtc gtcatctgct
     6961 tcacgtcggt ggcgctcgtc gcgatgatgt tcctcctgat ggcccggatg agcgatccgc
     7021 tcctgccgcg cgccctcttc ggggtgctca acacgatcct ctacttcccg agcggcgcgg
     7081 tgtaccccca gcaggcgttc cccgggtgga tgcgcgcgat cgcggtggcg gaccccttca
     7141 cctacgcggt gcacgccttc aaggcgctcc tcctcaagaa caccggcttc gccgcgatcg
     7201 ggaccgacct cctctatctg ggcctcttcg ccgcggtcac gatgaccggc gccacccttc
     7261 tcttccggcg gtcgctgtga gggggccgga cgggcgcggc tcgagcgagg acacccgccg
     7321 gcggctcttc gacgcggcgc gcgagctctt caccgaccag ggcttcgaca acgtgaccgt
     7381 gcgtgacctc tgcacgcgcg ccggcgccaa cctcgccgcc atcaactacc atttcggtga
     7441 caagctggcg ctctacctcg aggtggtgcg gagcgcggtc gaggagatgc ggagcacgaa
     7501 ccacgagatg atggcggggc cggagctccc ggccgcggac cggctccggc gctacgtccg
     7561 ggagtacgtg ccgcggctga tgctcatcga ccggcggcgc gcctggatcc agaagctgat
     7621 gcggaacgag atgtccgagc cgacggcggc ggcgcagctc atcatcgagg agatcctgcg
     7681 gccgcgggtc gcctacctca cggggatcgt ggcggagctc gtcggctgcg ccccggacga
     7741 cccccgcgtg acgcgctgcg tcagcgcgat ccagatgcag tgtttcgcct gcattc
//
LOCUS       DASUAP010000056         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_846201, whole genome shotgun sequence.
ACCESSION   DASUAP010000056 DASUAP010000000
VERSION     DASUAP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846201"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1318)
                     /locus_tag="VFU45_01380"
     CDS             complement(47..1318)
                     /locus_tag="VFU45_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357741.1"
                     /translation="MALLELGRAWAEEHIDFRVEVPPAPVTRDLVVMNGNEALGLGAV
                     AAGMELCAMYPITPATSVSHYLSEVFGRFGGKVHQAEDEIAAVGVAIGASYAGKVACT
                     VTSGPGLALKTEFLGLAAMTEMPLVLIDVQRGGPSTGLPTKVEQSDLLAALFASPGDA
                     PHVVVAPATIEECFHVMVTARRLAETFRAVVMVLSDANLATGVTPFPRPVPDARWQAA
                     PVDLAPVAEGTKAYQWNPVTGLSRRIVPGQPGGAYTVTGLSHDEGSKVAYSSGVHQRS
                     SAMRSRKLAVLQSMLTPPVTYGDPEGDLLIVGWGSTKGAIEEAVDRARAEGLKVSALH
                     LRFLSPLEPGLKGIFGRFGKVCTVELNYSDEPGEPFITEESRRRGQLAWILRAATLVD
                     VDCWTRVPGEPLRPGAILDAIRARLPRGGAK"
     gene            complement(1373..1939)
                     /locus_tag="VFU45_01385"
     CDS             complement(1373..1939)
                     /locus_tag="VFU45_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5357742.1"
                     /translation="MTVTRPAPRSRRAPRAVRKAEHTIEIVSDSGEGAQKCGQIFGAV
                     SAKMGNGVWTVEIIPAEIQPPPRVPEGASGYRIRFGVGAITNWGDATDLVLAFNEQVL
                     LARHRLGALAEDAVLLVEDKWATDDDAEIRAAWAAGMEELSHRRYRIIPVPMEAQCLT
                     LVDNPRKGKNMFALGLLACIYGRDLERI"
     gene            complement(1936..2211)
                     /locus_tag="VFU45_01390"
     CDS             complement(1936..2211)
                     /locus_tag="VFU45_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU5357743.1"
                     /translation="MTNRRTLRLSPSQDVRPVTEFRAHASAFLQQIRATKHPLILTQH
                     GRCAAVLLDISLYEELLGELAMLRGRRSTDPREPLPAPAPLVRTLPS"
     gene            complement(2334..>3218)
                     /locus_tag="VFU45_01395"
     CDS             complement(2334..>3218)
                     /locus_tag="VFU45_01395"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEU5357744.1"
                     /translation="LEALVRKEGAGKIAYVSLAATVNMAGGQPVSMANVKALRAFCDR
                     HKIRLFLDATRLAENAFFIQQREPGYAEVPIAKIVREFCDQTDGAWMSGKKDNLVNIG
                     GWLAVNDWTLFEELRNLIVVYEGLHTYGGMAGRDMEAMAVGIAESLEDDHIRSRVGQV
                     HYLGELLTDWDIPIVLPVGGHAIFLDAKAFYPHIPQSEFPAQTLAAELYLDSGIRTME
                     RGIVSAGRDPATGDHYYPKLELTRVTIPRRVYTQAHMDVVAESIKTIYDRRRETRGLR
                     MTYEPKYLRFFQAKFERL"
BASE COUNT          460 a         1080 c         1181 g          497 t
ORIGIN      
        1 tcatcctgct ccggctcgag gaccggcagg aagccgcagg cgacggtcac ttggcacctc
       61 cccgcggcag ccgtgcgcgg atcgcgtcga ggatggcccc gggacggagc ggctccccgg
      121 gcacgcgggt ccagcaatcc acgtcgacga gcgtggcggc ccggaggatc cacgcgagct
      181 ggccccgccg gcgcgactcc tcggtgatga agggttcgcc cggctcgtcg ctgtagttca
      241 gttccacggt gcacaccttc ccgaagcgcc cgaagatccc cttgagtccc ggctcgagcg
      301 gcgagaggaa ccggaggtgg agggccgaga ccttgagccc ctcggcgcgg gcgcggtcca
      361 ccgcctcctc gatggcgccc ttggtgctgc cccagccgac gatgaggagg tcgccctcgg
      421 ggtcgccgta ggtgacgggc ggcgtcagca tcgactgcag cacggcgagc ttccggctcc
      481 gcatcgcgga actgcgctga tgcacgccgg agctgtaggc caccttgctg ccttcatcgt
      541 gcgagaggcc cgtgacggtg taggcgccgc cgggctggcc ggggacgatg cgccgggaga
      601 gtcccgtcac cggattccac tggtaggcct tcgtcccctc ggccaccggg gccaggtcga
      661 ccggcgccgc ctgccaccgg gcgtcgggta ccgggcgggg gaacggcgtg acgccggtgg
      721 cgaggttggc gtcggagagc accatcacca cggcgcggaa ggtctcggcc aggcgccggg
      781 ccgtgaccat cacgtggaag cattcctcga tggtggcggg ggccaccacg acatgcggcg
      841 cgtcccccgg gctcgcgaag agcgccgcca ggaggtcgga ctgctccacc ttggtgggga
      901 gcccggtgct cggcccgccg cgctgcacgt cgatgagcac gagcggcatc tcggtcatcg
      961 ccgccaggcc gaggaattcg gtcttgagcg ccaggccggg ccccgaggtc accgtgcagg
     1021 cgaccttgcc ggcgtaagag gcgccgatgg cgacgcccac ggcggcgatc tcgtcctccg
     1081 cctggtgcac cttcccgccg aagcgcccga agacctcgct caggtagtgc gacaccgacg
     1141 tggccggggt gatcggatac atcgcgcaga gctccatccc cgcagccacg gcgccgagcc
     1201 ccagcgcctc gttcccgttc atcaccacca ggtcgcgggt gacaggcgcc ggcggcacct
     1261 ccacccggaa gtcgatgtgc tcctcggccc aggcgcggcc gagctcgagg agcgccacgt
     1321 tctgcgcata gaccgcctcg ctcttcttcc ggaaggcgta ggcgatctgt tcctagatcc
     1381 gctcgaggtc gcgcccgtag atgcaggcca ggagcccgag cgcgaacatg ttcttcccct
     1441 tgcggggatt gtcgaccagc gtgaggcact gcgcttccat cggcaccggg atgatccggt
     1501 agcgccggtg ggagagctcc tccatccccg ccgcccaggc ggcacggatc tcggcgtcgt
     1561 cgtcggtggc ccacttgtcc tcgaccagga ggacggcgtc ctccgccagt gcgccgagcc
     1621 ggtggcgcgc caggagcacc tgctcgttga aggcgagcac gaggtcggtg gcgtcgcccc
     1681 agttggtgat ggcgccgacg ccgaaccgga tgcggtagcc gctggcgcct tccgggacgc
     1741 ggggcggcgg ctggatctcg gcggggatga tctccaccgt ccagacgccg ttgcccatct
     1801 tggcggagac ggccccgaag atctggccgc acttctgcgc cccttcgccg gagtcggaga
     1861 cgatctcgat ggtatgctcg gccttccgga ccgcacgggg cgcgcggcgg gagcggggag
     1921 cgggtcgggt gacggtcacg aggggagggt cctcacgagt ggtgccgggg ccgggagcgg
     1981 ttcgcgcggg tcggtggacc ggcgtccgcg cagcatcgcg agctcgccca ggagctcttc
     2041 atacagggag atgtcgagca gcaccgccgc gcagcggccg tgctgggtca ggatgagcgg
     2101 gtgcttggtg gcgcggatct gctggaggaa cgccgaggcg tgcgcccgga attcggtgac
     2161 cgggcggaca tcctgggagg gactcagtcg gagcgtcctg cggttcgtca cagtgtacgc
     2221 ctgccggtac gcggagaatg aacgtgccgg acgttgtcac gcccccattg aagggggcgt
     2281 gaaggggcga tgagcaggtg aggaaggggg gcggagggcc ccggccccgg gagtcagagg
     2341 cgctcgaact tggcctggaa gaagcggagg tacttcggtt cgtacgtcat ccggaggccg
     2401 cgggtctccc ggcgccggtc gtagatcgtc ttgatcgact cggccaccac gtccatgtgg
     2461 gcctgggtgt acacccggcg ggggatggtc acccgggtga gctcgagctt cgggtagtag
     2521 tggtcgccgg tagcgggatc gcggccggcg ctcacgatcc cccgttccat cgtgcggatg
     2581 cccgagtcga ggtagagctc cgccgcgagt gtctgggcgg ggaattccga ctgcgggatg
     2641 tggggataga acgccttcgc gtcgaggaag atcgcgtggc ccccgaccgg caggacgatc
     2701 gggatgtccc agtcggtcag gagctcgccc aggtagtgca cctggcccac gcgggagcgg
     2761 atgtggtcgt cctcgagcga ttccgcgatg ccgaccgcca tcgcctccat gtcgcgcccc
     2821 gccattccgc cgtaggtgtg gagcccctcg tagaccacga tgaggttccg gagctcctcg
     2881 aagagcgtcc agtcgttgac cgccagccac ccgccgatgt tgaccaggtt gtccttcttg
     2941 ccgctcatcc aggcgccgtc ggtctggtcg cagaattcgc gcacgatctt cgcgatcggc
     3001 acctcggcgt accccggctc ccgctgctgg atgaagaagg cgttctcggc caggcgcgtg
     3061 gcatcgagga agagccggat cttgtggcgg tcgcagaagg cgcggagcgc cttgacgttg
     3121 gccatcgaga ccggctggcc tccggccatg ttgaccgtcg cggccaggct cacgtaggcg
     3181 atcttcccgg ccccttcctt ccggaccagc gcctcgag
//
LOCUS       DASUAP010000057         5500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_968933, whole genome shotgun sequence.
ACCESSION   DASUAP010000057 DASUAP010000000
VERSION     DASUAP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968933"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFU45_01400"
     CDS             complement(<1..236)
                     /locus_tag="VFU45_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357745.1"
                     /translation="MHPLAGHLALRRKLSAALAADHLPQLLLFTGPAGVGKQRLALWL
                     AQSTLCAAPAGGEPCGTCPSCRLVLELSHPDLHW"
     gene            712..784
                     /locus_tag="VFU45_01405"
     tRNA            712..784
                     /locus_tag="VFU45_01405"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:745..747,aa:Asn,seq:gtt)
     gene            complement(876..1655)
                     /locus_tag="VFU45_01410"
     CDS             complement(876..1655)
                     /locus_tag="VFU45_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357746.1"
                     /translation="MDRNQKLVRILFATGIVCVAGLVLVYGYSVLLFQEVPPAWAGWL
                     TTVGYASAAILAVTGFGLLFDRSAQLSVRILLPFLLLWLLTRIPVVLADPLREISWFA
                     VGEVAVPFSAALILFTRLADPGTTSRIRRAAVTHGPIVTRVLVGASLVTFGLSHFFEF
                     AARTVSLVPAWLPYRTAWADLAGAAQVAAGLGVLFKVYPRLAAAAEAAMLSLFTILVW
                     IPAVIARPGLQSNWVEFLFTWALAAACWVVAESIPARHVGA"
     gene            complement(1793..2692)
                     /locus_tag="VFU45_01415"
     CDS             complement(1793..2692)
                     /locus_tag="VFU45_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEU5357747.1"
                     /translation="MPELPDITVYVEALGRRVTGQPLQHARLRSPFLLRSVSPPIADA
                     EGRTVRAVTRLGKRVVLELEGELFLVLHLMIAGRLHWKAAGAKPAGKTGLAAFDFPAG
                     TLTLTEAGTKRRASLHLVRGREELRQFDRGGLEPLATSVADLLARLRSENHTLKRALT
                     DPRMLSGVGNAYSDEILHRARLSPLRLTSKLTDVEGARLHAALRVVLVEWTDRLRREV
                     GDGFPEHVTAFRAGMAVHGRFREPCPVCGTPVQRIRYAENETNYCPACQTDGRLLADR
                     AMSRLLREDWPRSLEEMDERLGK"
     gene            complement(2710..4563)
                     /locus_tag="VFU45_01420"
     CDS             complement(2710..4563)
                     /locus_tag="VFU45_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU5357748.1"
                     /translation="MRARIRFALTYFVCWVAYFEVGRLLFLGWYWQDTATLGFGTFLG
                     VLFHGLRMDAASAAYLMLVPVLALMATSYVPWKFVRWLVAGWTGLFVVVVAALTIGDL
                     GLFREWGFRLDSTWLMYINRPKEMIASAGSSPVTALLSVFVAYAALGFWTFRRWALPT
                     ADDPDLRPVGWPVLPAGVFSVAVLAFVSRGGFQLTPLTHSSVYFSDNDFADQATLNVG
                     WNFFYSVNHHEYQKANPYAFLPAAEAKARADTLLAPDRGTPPRLFRLAHPNVIVVIWE
                     SFSAKLAASTGGRSGVVPEFDSLARTGILFDSIYASGNRTEKGLPAIMSGYPALGNES
                     ILKSPRKFSSLPGLARDLGAAGYDSRFLYAGELAWANFRYYLRDAGFRTVSGEEAFSG
                     DRFRTKWGYHDQVLFERAGGIVDSLARPFFFGLLTLSSHDPYDIPVAHAFPGNDVGQL
                     FLSAHHYSDSTLGAFVRALAARPVWDSTVVIILADHGHRLPELDPPADLSDPSAFHIP
                     MLWLGGALAVHDTVIHTIGSQTDLASTLLDQLDVPRTHYIWGDDLLLSGHRPDAYYGF
                     HDGFGYLDARGGFTYNDVGRRVMQQWGTVDSADVRNGMALQQQLVADYEAR"
     gene            complement(4563..>5500)
                     /locus_tag="VFU45_01425"
     CDS             complement(4563..>5500)
                     /locus_tag="VFU45_01425"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU5357749.1"
                     /translation="LPAPRRRIYQAARAVPAGATVSYGELAASIGAPGAARAVGAAMA
                     DNPFILAIPCHRVMGSNGQLHGFSAHGGIETKRRILALEGVRVYIPRTAPRRYPDGLE
                     YDRAAAVDHLRRADPVLARLIKRAGPYVLTVRRMHDSFQSLVRSIVYQQLSGKAAETI
                     LGRVLDLYAPKDFPTPDDILATTDATLRGAGLSAGKTRALKDLATRTNEGVVPPLRAL
                     HRMGDDEVIERLTSIWGVGRWSVEMMLMFRLGRPDVLPVDDLGVRQGFDLTYGRRRPV
                     SRDQLAEHAEAWRPYRSVASWYMYRAVDLHRKGDA"
BASE COUNT          899 a         1734 c         2084 g          783 t
ORIGIN      
        1 aaccagtgga ggtccgggtg agagagctcg aggaccaggc ggcaggaggg gcaggtgccg
       61 cagggctcgc cgccggcggg ggcggcacat agggtcgact gggcgagcca gagcgccagg
      121 cgctgcttcc ccaccccggc cgggccggtg aacaggagga gttgggggag gtggtcagcc
      181 gcgagcgcgg cggagagctt ccgccggaga gcgaggtggc ctgccagggg gtgcatggtc
      241 acgaaagaag gatagacggg tgggagttcc cggggaactg gctacagcgt aacagatttt
      301 cccacaatga gttaggtcgc gtttttttgg cctcacccgg cactatttcg gtgcatttgt
      361 gcctcgatga aattgtgtga gcggttgctc gcaacgcaat gatgtgagtg actgctcgct
      421 atttatgcaa atcattgttt ctaatagcgt tagctatgtg gtgctggcgg agcccagccg
      481 ttggtcgaaa tcgggcaaag cgctggcggt tgactgtgaa gttgctgtga tgacggtggc
      541 gctcgagggg cgattcgtgc tgctggtggt gtgcgcgccc tggtcgaaga tccgccgctg
      601 gagggtgctc cgacgaacgg tcatcgccgc cgctgcaccg atcgagtgag tgcgggtgag
      661 agtgatgcgt cgcttgattc tgctggtgag cgcccttagt ttgccgcctc ttcccgagta
      721 gctcagttgg tagagcagcg gactgttaat ccgcgggtcg caggttcgag tcctgcctcg
      781 ggagctcgag cgccgcaagc cattgcttgc ggcgctttga cgttcggggc gggtcgtgcg
      841 cgccctgtga tcccggagcc ggcgggcccg ccagctcagg cgccgacgtg ccgagccggg
      901 atgctctcgg ccacgaccca gcacgccgcc gccagcgccc acgtgaagag gaactccacc
      961 cagttggact ggagcccggg ccgggcgatg accgccggga tccagacgag gatcgtgaac
     1021 aggctcagca tcgcggcctc cgccgcggcg gcgaggcgcg ggtagacctt gaagagcacg
     1081 cccaggccgg ccgcgacctg tgcggcgccg gccaggtcgg cccaggccgt ccggtagggc
     1141 agccacgccg gcaccagcga cacggtgcgg gcggcgaatt cgaagaaatg ggacagcccg
     1201 aacgtcacga gcgacgctcc cacgaggacg cgcgtgacga tcggcccgtg tgtgaccgcc
     1261 gcccgtcgga tcctcgacgt ggtgcccggg tcggcgaggc gagtgaagag gatcaatgcc
     1321 gccgagaagg gcacggcgac ctcgccgacg gcgaaccagc tgatctcccg gagcggatcg
     1381 gcgaggacca ccgggatgcg ggtgaggagc cagaggagga ggaatgggag gaggatgcgc
     1441 accgacagct gggcggatcg gtcgaagagg aggccgaacc cggtgaccgc gagtatggcg
     1501 gcggaggcgt agccgacggt tgtcagccat ccggcccacg ccggcgggac ctcctggaac
     1561 aggagcactg aatagccgta caccagcacg agcccggcta cgcagacgat gccggtggca
     1621 aagaggatcc gtacgagctt ctggtttcgg tccacgggcg ccctccacgg tgagcgaccg
     1681 gcgcgggtgc gcaggcctgt tacggcgggt gacatcggga cggagacgag cggccctccg
     1741 gcggaatgac atcacatgac ccggggcgcc gccggccccg aaccctcctc cgctatttcc
     1801 ccagccgctc gtccatctcc tccaagctcc tcggccaatc ctccctcagc agccgcgaca
     1861 tcgcacggtc ggccaggagc cgcccgtcgg tctggcacgc ggggcagtaa ttggtctcgt
     1921 tctcggcgta ccggatccgc tggaccggcg tgccgcacac agggcagggc tccctgaagc
     1981 ggccgtggac cgccattccc gcccggaagg cggtgacgtg ctccgggaag ccgtcaccca
     2041 cctcccgccg gagccggtcg gtccactcga caaggacaac cctgagcgcg gcgtggaggc
     2101 gcgctccttc gacgtcggtg agcttcgagg tgagtcggag gggcgacagc cgggcgcgat
     2161 ggaggatctc gtcggagtag gcatttccga cgccgctcag catccgggga tcggtgagcg
     2221 cccgcttgag ggtgtgattc tcgctccgca ggcgagcgag caggtccgcc accgacgtgg
     2281 cgaggggctc gaggcccccg cgatcgaact ggcgcagctc ctcgcggccc ctcaccaggt
     2341 gcagtgacgc gcggcgcttc gtgcccgctt cggtgagggt gagtgtcccc gcggggaagt
     2401 cgaacgccgc gagcccagtc ttgccggcgg gcttggcgcc cgccgccttc cagtggagcc
     2461 gcccggcgat catcaggtgg aggacgagga acagctcgcc ttcgagctcg aggacgaccc
     2521 gcttaccgag gcgagtgacg gcgcggaccg tgcgcccttc ggcatcggcg atcggaggtg
     2581 agacgctgcg gaggaggaac gggctccgga gacgcgcgtg ctgcagcggc tggcccgtga
     2641 cgcggcgtcc gagcgcctcg acgtacacgg tgatgtcggg gagctcaggc atcgccgtcg
     2701 gtccggcgcc tagcgcgcct cgtagtcggc cacgagctgc tgctgcagcg ccatcccgtt
     2761 gcgcacgtcg gcactgtcga ccgtgcccca ctgctgcatc acgcggcggc cgacgtcgtt
     2821 gtaggtgaat ccgccgcggg cgtcgaggta cccgaatccg tcgtggaagc cgtagtaggc
     2881 gtcagggcgg tggccggaaa ggaggaggtc gtcgccccag atgtagtgcg tccgggggac
     2941 gtcgagctgg tcgaggaggg tcgaggccag gtcggtctgg gatccgatgg tgtgaatgac
     3001 ggtatcgtgg actgcgaggg cgccgccgag ccagagcatc ggaatgtgga atgcgctcgg
     3061 gtcggagaga tcggccggcg ggtcgagctc cggcagccgg tggccgtggt cggccaggat
     3121 gatgacgacg gtgctgtccc acaccggcct ggcggcgagc gcccgtacga aggcgccgag
     3181 ggtcgagtcg ctgtagtggt gggcgctcag gaagagctgg cctacgtcgt tgccggggaa
     3241 ggcgtgggcc accgggatgt catagggatc gtggctcgac agcgtgagca ggccgaagaa
     3301 gaacggccgc gcgagcgagt cgacgatccc gccggcgcgc tcgaagagca cctggtcgtg
     3361 gtacccccac ttggtgcgga agcggtcgcc cgagaaggct tcctcgccgg acacggtccg
     3421 gaagccggca tcgcggaggt agtagcggaa gttggcccag gccagctcgc cggcgtacag
     3481 gaagcgcgag tcgtacccgg cggcgccgag atcgcgggcg agccccggca gcgatgagaa
     3541 cttccgcggc gacttgagga tcgactcgtt gccgagcgcg gggtagccgc tcatgatggc
     3601 cggaagcccc ttctcggtgc gattgccgct cgcgtagatg ctgtcgaaga ggatgccggt
     3661 gcgggccaat gaatcgaact cgggcaccac gccgctccgg ccgccggtcg aggccgcgag
     3721 cttggcggag aagctctccc agatgaccac gatcacgttg gggtgcgcca ggcggaagag
     3781 gcgcggcggc gtgccccggt ccggcgcgag cagcgtatcg gcgcgggcct tggcctccgc
     3841 ggcgggcagg aaggcgtagg gattggcctt ctggtactcg tggtggttga cggagtagaa
     3901 gaagttccag ccgacgttga gcgtggcctg gtcggcgaag tcgttgtcgc tgaagtagac
     3961 gctgctgtgc gtgagcgggg tgagctggaa gccgccgcgc gacacgaacg ccagcacggc
     4021 gacgctgaac acgccggcgg ggaggaccgg ccaaccgacg ggccggaggt cggggtcgtc
     4081 ggcggtcggg agcgcccagc gccggaaggt ccagaacccg agcgctgcgt aggcgacgaa
     4141 gacgctgagg agcgccgtga cgggcgagga gccggccgag gcgatcatct ccttgggccg
     4201 gttgatgtac atcagccagg tgctgtcgag ccggaagccc cactcccgga agagccccag
     4261 gtcgccgatg gtgagcgccg ccaccaccac gacgaagagc cccgtccacc cggcaacgag
     4321 ccagcgcacg aacttccacg gcacgtacga cgtggccatc agcgccagga ccgggacgag
     4381 catcaggtac gcggccgagg cggcatccat tcggaggccg tggaagagca cgcccaggaa
     4441 ggtgccgaag ccgagcgtgg cggtatcctg ccagtaccag cccaggaaga gcaggcggcc
     4501 cacctcgaag taggccaccc agcagacgaa atacgtcagc gcaaagcgga tgcgggcccg
     4561 catcaggcgt ctcccttccg atgcaggtcc accgcgcggt acatgtacca gctcgccacc
     4621 gagcgatacg gccgccacgc ctcggcgtgt tccgccagct ggtcgcggga caccgggcgg
     4681 cgccgcccat aggtgaggtc gaagccctgg cggacgccga ggtcgtcgac cgggaggacg
     4741 tcggggcggc cgagccggaa catcagcatc atctccaccg accagcggcc cacgccccag
     4801 atggaggtga ggcgctcgat gacctcgtca tcgcccatcc ggtgaagcgc gcggagcggc
     4861 ggcaccacgc cctcgttggt gcgtgtggcg aggtccttga gcgcgcgggt cttgccggcg
     4921 gagagcccgg cgccgcggag cgtggcgtcg gtggtggcaa ggatgtcgtc cggggtgggg
     4981 aagtccttcg gggcgtagag gtcgagcacc cgtcccagga tcgtctcggc ggccttgccg
     5041 ctcagctgct ggtagacgat cgaccgcacc agcgactgga aggagtcatg catccggcgg
     5101 acggtgagga catacggccc cgcacgcttg atgaggcgcg cgagcaccgg gtccgcgcga
     5161 cggaggtggt ccacggccgc ggcgcgatcg tactcgaggc cgtcgggata tcggcgcggg
     5221 gcggtgcgcg ggatgtagac ccgcacccct tcgagcgcca ggatgcggcg tttggtctcg
     5281 atcccgccgt gcgcggagaa cccgtggagc tggccgttgc tccccatcac ccggtggcag
     5341 gggatcgcca ggatgaaggg attgtcggcc atcgccgcgc cgaccgcgcg tgccgcgccg
     5401 ggcgcgccga tgctcgcggc gagctcgccg taactgaccg tggcccccgc cggcaccgcc
     5461 cgggcggcct ggtagatccg tcgccgcggg gcggggaggc
//
LOCUS       DASUAP010000058         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1268535, whole genome shotgun sequence.
ACCESSION   DASUAP010000058 DASUAP010000000
VERSION     DASUAP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268535"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFU45_01430"
     CDS             complement(<1..992)
                     /locus_tag="VFU45_01430"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEU5357750.1"
                     /translation="MSVLRRYQGAQAVRGPLVFLRRTPAAVLGEWVTLRSPNASPRRG
                     QVIDAGEDVSVIQVLEDTVGLAPARTELELTGREAHVVVGRELLGRVLTGTGDPRDGL
                     PAPIGESLRPTWGAPLNPVRRARPAEMIETGISAIDGMNTLVRGQKLPVFSGPGLPGL
                     EVAARIVAGARAPRGEPFAVVFGGIGITARELEYFVSSVEASGALPRTVLYLNETRDP
                     TIERLLAPRAALAQAEFLAFEAGMHVLVVLADMTNYCEALREIATAREEMPGRRGYPG
                     YMYTDLASLYERAGIIEGGSGSLTQIPIVTMPDDDITHPIPDLTGYITEGQVVL"
     gene            complement(1007..2743)
                     /locus_tag="VFU45_01435"
     CDS             complement(1007..2743)
                     /locus_tag="VFU45_01435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEU5357751.1"
                     /translation="MTVTTPRVVRASGSLVEARPLPQAALYELVRVGERGLLGEVVRL
                     SDDLATVQVYEETVGLKVGEPVTLAGETLAVTLGPGLLGSVLDGVGRPLAALAAAHGD
                     FIGPGLVAPTLDRARRWKFSASIAPGRTVAPGDVIGVVQEREGFEHRVMVPPGVSGVV
                     EAIASGEYDVTQAVGRIAGGVELRLAQAWPVRIPRPAAARLPAERPFLTGQRIFDLLF
                     PVAEGGTVAVPGGFGTGKTVIEQSLAKYADADVIVYIGCGERGNEMAEVLHEFPRLAD
                     PRTGRTLADRTVLVVNTSNMPVAAREASVFVGVTIAEYYRDMGYRVALMADSLSRWAE
                     ALREMGARLQEMPGEEGYPTTLGNRLGKFYERAGRVRAVGLPERTGALTIINAVSPPG
                     GDFSEPVTQASLRVAGALWALDPGLAHQRQFPAVDWNASYSLYADALAPWFASASGAA
                     WDDLRRGVLKLLAEERELREIAGLVGPEALQDADRLRLEVARAIRENVLGQSAFDPND
                     AASSIDKTRRLAEAGLECLRAGLAALQSGGSFEQMDLPAAVRALADLRRAAPDQVRAA
                     AENALAASRTLA"
     gene            complement(2740..3069)
                     /locus_tag="VFU45_01440"
     CDS             complement(2740..3069)
                     /locus_tag="VFU45_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEU5357752.1"
                     /translation="MSHSVRILARPETAAGFRLAGLTPRVTEADAGELSASRAEMRTA
                     DAGVILMDDRIFQRLPAEDRRRLGRRPLPIVVPFPTPEWRTEREAAEAYVLELLRQVV
                     GYRVRLK"
     gene            complement(3066..>3273)
                     /locus_tag="VFU45_01445"
     CDS             complement(3066..>3273)
                     /locus_tag="VFU45_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357753.1"
                     /translation="YGSVFRKGSRPGLEDALLDARLEVQRRAARAAPLGVAPVLWFAL
                     ELRRESTQISRLIWALALGAPVSA"
BASE COUNT          518 a         1200 c         1089 g          466 t
ORIGIN      
        1 ctcaatacga cttgcccctc ggtgatgtag ccggtgagat cgggaatggg atgcgtgatg
       61 tcgtcgtccg gcatcgtcac gatggggatc tgcgtcagcg atcccgatcc accctcgatg
      121 atcccggcgc gttcatacag actggcgagg tcggtgtaca tgtaacccgg ataaccccgc
      181 cggcccggca tctcctcgcg cgcggtggcg atctcccgga gggcctcaca gtagttcgtc
      241 atgtccgcca gcacgacgag cacgtgcatg ccggcctcga aggccagaaa ttcggcctga
      301 gccagggcgg cccggggtgc cagcagtcgc tcgatggtcg gatcccgcgt ctcgttgagg
      361 tagaggacgg ttcgcgggag cgctccactg gcttccacac tcgagacgaa atactcgagc
      421 tcgcgggccg taatgccgat gcccccgaac accaccgcga agggttcgcc ccggggcgcc
      481 cgggcgcccg cgacgatacg cgcggccact tcgagaccgg gcaatcccgg accggagaag
      541 accggaagct tctgcccgcg taccagcgtg ttcatgccgt cgatggccga gatgccggtt
      601 tcgatcatct cggcaggccg ggcgcgccgg acggggttca agggcgctcc ccacgtgggt
      661 cgcaggctct ccccgatggg cgctggaagg ccatcccgcg gatccccggt gccggtgagc
      721 actcggccca gcaactcgcg cccgaccact acgtgggcct cacgacccgt cagctccaac
      781 tcggtccggg cgggggccag gcccaccgtg tcctccagca cctgaatgac cgatacgtcc
      841 tcaccggcat cgatcacctg cccccggcgc ggagaggcgt ttggggagcg gagcgtcacc
      901 cattcgccga gcaccgccgc gggtgtccgc cggaggaata ccaggggacc cctcaccgcc
      961 tgtgcgccct ggtatctccg cagaacgctc atggagcggc actcgcttag gcgagggtcc
     1021 ggctggcggc gagtgcgttc tcggccgctg cccggacctg atccggcgcg gcgcggcgca
     1081 agtcggccaa cgcccggacc gcagccggga ggtccatctg ctcgaacgac ccgcccgact
     1141 ggagtgccgc gagcccggcg cggagacact cgaggcccgc ttccgccaac cgacgcgtct
     1201 tgtcgatcga cgatgccgcg tcgttcggat cgaaggcact ctggcccagt acgttctccc
     1261 gaatggcgcg cgcgacctcg agccgcagcc ggtcggcatc ctgcagcgcc tcagggccaa
     1321 ccagccccgc gatctcgcgc aactcccgtt cttcggcgag gagtttgagc acaccccggc
     1381 ggagatcatc ccacgcggcg cccgacgcgg acgcgaacca cggcgccaac gcatccgcgt
     1441 acaggctgta gctcgcgttc cagtcaaccg ccgggaactg ccgctggtgg gcgagccccg
     1501 ggtcgagggc ccacaacgcc ccggccaccc gcaacgaggc ctgggtgacg ggctcggaga
     1561 agtctccgcc aggtggggat accgcgttga tgatggtgag ggcaccggtg cgctccggca
     1621 ggccgacggc ccgcacccgt ccggcccgct cgtagaactt cccgagccgg ttgcccaagg
     1681 tcgtggggta tccttcttcg cccggcatct cctgcagccg ggcgcccatt tcgcggaggg
     1741 cctcggccca gcgcgacagg ctgtcggcca tcaacgccac ccgatacccc atgtcccggt
     1801 agtactcggc gatggtaacg cccacgaaga cggacgcctc gcgcgccgcc accggcatgt
     1861 tggacgtgtt caccaccaag accgtccggt cggcgagcgt ccgcccggtc cgaggatccg
     1921 cgagccgggg gaactcgtgg agcacttcgg ccatttcgtt cccgcgctct ccgcagccga
     1981 tgtagacgat gacgtccgcg tcagcgtact tcgcgagtga ttgctcgatg accgtcttgc
     2041 cggtcccgaa gccgcctggc accgcgacgg tcccgccctc ggccaccggg aagagcaggt
     2101 cgaatatccg ctggcccgtg agaaacggcc gctcggcggg cagcctcgcc gccgctggac
     2161 gtgggatccg caccggccat gcctgtgcga gccgcagctc cacgcctccg gcgattcgac
     2221 cgacggcctg ggtcacgtcg tactcgccgc tcgcgatggc ctcgaccacc cccgacacac
     2281 cgggcggcac catgacccga tgttcgaacc cttcccgttc ctgaaccacc ccgatcacgt
     2341 cccccggggc cacggtgcgt ccgggggcga tcgacgcgct gaacttccag cggcgcgctc
     2401 gatcgagggt cggcgccacc aacccgggtc cgatgaagtc accgtgcgcg gcggccagcg
     2461 cggccagagg acgtccgacg ccatccagca cgctgccgag caaccccggc cccagcgtga
     2521 cggccagggt ctcaccggct agggtcaccg gttccccgac tttcaggccc accgtttcct
     2581 catagacctg cacggtggcg aggtcgtccg acagacggac gacctcgccc agaagaccgc
     2641 gctctccgac tcgaaccaac tcgtagagcg cggcttgggg caggggccgg gcctcgacca
     2701 acgaacccga tgcgcgcacc acccgaggcg tggtgaccgt catttcagcc ggacccggta
     2761 cccgacgacc tgccggagca actccaggac ataggcctcg gccgcttccc gctccgtccg
     2821 ccactccggc gtcgggaagg ggaccacgat ggggagtggc cgacggccca gccgccgccg
     2881 gtcctcggcg gggagccgct ggaagatccg gtcgtccatg aggatcacac cggcgtcggc
     2941 ggtgcgcatc tccgcccggg acgcagacag ctcccccgcg tcggcctcgg tcacgcgcgg
     3001 tgtgagacct gccaggcgaa agccggcggc ggtttcaggc cgggccagaa tccgtaccga
     3061 gtgactcatg cgctcaccgg cgcgccgagc gccagcgccc agatcaagcg gctgatttga
     3121 gtggattccc ggcggagttc gagtgcgaac cagagcacgg gcgcgacgcc caagggggct
     3181 gcgcgcgcgg cccggcgttg gacctcgagg cgggcatcga gcagggcgtc ttcgagcccg
     3241 ggccgggatc cctttcggaa gacggatccg tag
//
LOCUS       DASUAP010000059         4384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1023178, whole genome shotgun sequence.
ACCESSION   DASUAP010000059 DASUAP010000000
VERSION     DASUAP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023178"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFU45_01450"
     CDS             <1..228
                     /locus_tag="VFU45_01450"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HEU5357754.1"
                     /translation="TDPLPVQAVPPVVPGAASARLLEGIPPGAAETIRFTAPDGEYRL
                     FCAVPGHGLAGMWLRVRVSKRVRAPALRATP"
     gene            complement(225..1787)
                     /locus_tag="VFU45_01455"
     CDS             complement(225..1787)
                     /locus_tag="VFU45_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5357755.1"
                     /translation="MTDHAAPLSGPDLAAGIVLADIPDGGILLGHTQGEAVLLSRSGQ
                     EVFAIGAVCTHYGGPLNEGLVVDGTVRCPWHHACFSLRTGEALAAPALRPVDRWVVER
                     RGDAVLVRAKAPPFAPRRLTGAGPGSIVILGAGAAGAAAAERLRREGYGGPLTLVDAE
                     PDGPVDRPNLSKDYLAGTAPEAWIPLFPDDWFSRHRITRRFGVRAGAIDPAARVVTLA
                     DGERLSFDRLLIATGADPARLPIPGADLPHVHTLRSLGDSRTLIAAAAKARRAVIVGS
                     GFIGLEVAASLRARGLEVDVVTPDSRPLQLQLGSELGGFVRQLHEEHGVRFHLRAGVE
                     AITPGGVRLVGGGQVPAELVVLGVGVRPAVGIASAAGLTVDGGILVDEFLETSVPGIF
                     AAGDVARWPDPRSGGRVRVEHWAVAQRQGQVAALNLLGGREPFRAVPFFWSQHYDVTI
                     AMVGDPAGCDRVVARGAPATRYCLVAFHRKGKVAAIATIGRDRESLLAEAAFERGDEV
                     ALSALVADGGPA"
     gene            complement(1879..2418)
                     /locus_tag="VFU45_01460"
     CDS             complement(1879..2418)
                     /locus_tag="VFU45_01460"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU5357756.1"
                     /translation="MKQLFLPLAALVALAAFAPRRVAPAMQASAALDDATIVAIFDAA
                     NTADIETGDLAAKMGHDKRVRALGAQFSHDHATVRQQGRDLAKKLGVTPTPPADDKSA
                     ADQAAVMKMLRSKKGAAFDKAYLQHEVAFHAAVIDAVTKTLLPAIQNAELKAFVEKVA
                     PAFQGHLLAAQELEKQLGD"
     gene            2558..3190
                     /locus_tag="VFU45_01465"
     CDS             2558..3190
                     /locus_tag="VFU45_01465"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5357757.1"
                     /translation="MVSTVWNQRFLKTTRGRIVDLLRREPRTAEALARAIGLTPNAVR
                     GHLALLERDGMIQADGMRRGVGKPAHIYRLTAAAEAQFSKAYAPMLRGLLDELASRLD
                     PAQLEELLRATGQRLAPRAGAARDASDEQCIAAAEAALAELGGVIASVTANGTIQIDG
                     LACPLAEVTGTHAAVCRGIEALLSGATGRTVTERCERTGGRARCRFEMGK"
     gene            complement(3346..>4384)
                     /locus_tag="VFU45_01470"
     CDS             complement(3346..>4384)
                     /locus_tag="VFU45_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5357758.1"
                     /translation="RLDNIPRLSLPVGEAGVSPFTLTAGSRGGDQVLMAEDVKVAVGD
                     RVLLDRFSSRVKRGDVIGFVGANGAGKTTLLKTLLGERPAEGGTVRVGDGTVVAYYQQ
                     DLGQVPADKTIYDIIADLRPKWTRGAVQGHLGTFGFSGDSVLRVAGSLSGGERARVAL
                     AMMALAEANLLVFDEPTNHLDVESIESLEESLEAYEGTVILVSHDRALLRNLVTRVWA
                     LENARIDDVEGTFDDWEAFRAAREKRARTAAAEARQAETKRPRQKADPEEERRARQSA
                     ERTAGRKRAAAETRVQQLEGAVRELESRLADPALYQGKNGAAEAARLNAELSARKDDL
                     DRAMEDWAALN"
BASE COUNT          630 a         1552 c         1588 g          614 t
ORIGIN      
        1 acggacccgc tgccggtgca ggccgtcccg cccgtggtac ccggggccgc gagcgcccgg
       61 ctcctcgagg ggatcccgcc gggagcggcc gagacgatcc ggttcaccgc acccgacggc
      121 gagtaccggc tcttctgcgc ggtcccgggc cacgggttgg cggggatgtg gctgcgagtg
      181 cgcgtctcga agcgggtgcg cgcgcccgcg ctccgggcga cgccctaggc cggcccgccg
      241 tcggccacga gcgcgctgag cgccacctcg tccccgcgct cgaaggcggc ctccgccagc
      301 aggctctcgc gatcccggcc gatcgtggcg atcgccgcca ccttcccctt ccggtggaac
      361 gcgacgaggc agtagcgtgt cgccggagcg ccacgcgcca cgacccggtc gcagccggcg
      421 ggatcgccca ccatggcgat ggtcacgtca tagtgctggc tccagaagaa cggcacggcg
      481 cggaagggct cgcgtccacc gagaaggttg agcgcggcga cctgaccctg ccgttgcgcc
      541 accgcccagt gttccacccg cacgcggcca ccggaccgcg gatcgggcca gcgggccacg
      601 tcgcccgcgg caaagatccc ggggacgctc gtctccagga actcgtcgac caggatcccg
      661 ccgtcgactg tgagccccgc ggcggaggcg atgcccaccg cgggccggac gccgaccccg
      721 agcaccacaa gttccgccgg gacctgcccg cccccgacca gccgcacccc gcccggcgtg
      781 atcgcctcga cgcccgcgcg caggtggaac cgcaccccgt gctcttcgtg gagctggcgg
      841 acgaacccgc ccagttcgct gccgagctgg agctggagcg gccgggagtc gggggtgacc
      901 acatccacct cgaggcccct cgcgcggagc gatgccgcga cctcgagacc gatgaacccc
      961 gacccgacga tcacggcgcg ccgggccttt gcggcggcgg cgatgagcgt gcggctgtcg
     1021 ccgagagagc gcagcgtatg gacgtgcggc aggtcggccc cggggatcgg gagcctggcg
     1081 ggatcggcgc cggtggcgat caggagccgg tcaaaggaca ggcgctcgcc gtccgccagc
     1141 gttacgacgc gtgcggcggg atcgatggcc ccggcgcgca cgccgaaacg gcgggtgatc
     1201 cggtgccggg agaaccagtc gtccgggaag agggggatcc acgcctcggg ggcggtgccg
     1261 gccaggtagt ccttggaaag gttgggacgg tcgaccggtc cgtccggctc ggcgtcgacc
     1321 agcgtcagcg gcccgccata cccttcgcgc cgcaaccgtt cggccgcggc ggccccggcg
     1381 gcgccggccc cgaggatcac gatcgaaccg ggacccgcgc ccgtcagtcg gcggggagcg
     1441 aacggcggcg ccttggcccg gaccagcact gcatcgccac ggcgttcgac cacccagcga
     1501 tccaccggcc ggagcgcggg tgcggcgagc gcctccccgg ttctgaggct gaagcaggcg
     1561 tggtgccagg ggcagcggac ggtgccgtcg accacgagcc cttcgttgag cggaccgccg
     1621 tagtgggtgc agacggcgcc gatggcgaag acctcctgcc cgctgcggga gagcaggacc
     1681 gcctcgccct gggtgtggcc cagcaggatc ccgccgtcgg ggatgtcggc cagcacgatg
     1741 ccggcagcga ggtcaggtcc ggacaagggt gcggcgtggt cggtcatggg atgtccgtga
     1801 aggaaggcct accggggaac gagcgccgct ccccggaggt tccggtgggc cccgcccggt
     1861 tggccgggcc ccgccggctc agtctccgag ctgcttctca agttcctgcg cggcgagcaa
     1921 atggccctgg aacgcgggag ccaccttttc cacgaaggcc ttgagttcgg cgttctggat
     1981 cgcggggagg agcgtcttgg tgaccgcgtc gatgacggcg gcatggaagg ccacttcatg
     2041 ctgcaggtag gccttgtcga atgccgcacc cttcttgctc cggagcatct tcatcaccgc
     2101 cgcctggtcg gcagcgctct tgtcgtcggc cggcggcgtc ggggtgacac cgagcttctt
     2161 ggcgaggtcc cggccctgct ggcgcaccgt ggcgtgatcg tgggagaact gcgcgccgag
     2221 cgcgcggacc cgcttgtcgt ggcccatctt cgcggcgagg tcgccggtct cgatgtcagc
     2281 cgtgttggcg gcgtcgaaga tggcgacgat cgtggcgtcg tcgagcgcgg cggacgcctg
     2341 catcgccggg gcgacgcgcc ggggggcaaa ggcggccagc gcgaccagcg cggcgagggg
     2401 aaggaacagt tgcttcatag ggacctccgg aacagggtga cgggatcgga acggccgcag
     2461 ctgcagcagc ccgacctcaa gatagacggt tacaccaatt acacaagtat ttacttgtct
     2521 aattgacata actccatcct gaacgtagtt ttaggtcatg gtcagcacgg tctggaatca
     2581 acgattcctc aagacgaccc gcggcaggat cgtcgacctg ctccggcgcg agccgcgcac
     2641 cgcggaggcg ttggcgcggg cgatcggact gacgcccaac gcggtgcggg ggcacctggc
     2701 gctgctcgag cgcgatggga tgatccaggc cgatgggatg cggcgtgggg tgggcaagcc
     2761 ggcgcatatc taccgcctga cggcggcggc ggaggcgcag ttctcgaagg cctacgcgcc
     2821 gatgctgcgg gggctgctgg acgagctcgc atcgcgcctg gacccggccc agctcgagga
     2881 attgctgcgc gccaccggtc agcggctggc accccgcgcg ggggcggccc gcgacgcctc
     2941 ggacgagcaa tgcatcgcgg cggcagaggc ggcgctggcg gagctcggcg gcgtcatcgc
     3001 gtcggtgacc gcgaacggga cgatccagat cgacgggctg gcgtgccccc tcgcggaggt
     3061 gaccggcacg cacgcggcgg tgtgccgcgg catcgaagcc ctcctgtcgg gggcgacggg
     3121 gcgcacggtc acggagcgat gcgagcgtac cggagggcgg gcccgctgcc ggttcgagat
     3181 ggggaaatag agctatcagc tatcagctat cagctatcag ctatcagcta tcagctatca
     3241 gctatcagct atcagctatc agcgcaggaa gatcgacgat atgaccaccc ccgcaaggcg
     3301 tgcgggggtt tgtcgtttcg gaggcggcgg caggcgaccg gcacatcagt tcaacgcggc
     3361 ccaatcctcc atcgcgcggt cgaggtcgtc cttccgcgct gaaagttcgg cattcaggcg
     3421 tgccgcttcg gcggccccgt tcttcccctg atacagcgcc gggtcggcga gccgcgattc
     3481 gagctcgcgc acggcgccct ccagctgctg cacccgcgtc tcggcggccg cgcgcttgcg
     3541 cccggcggtc cgctccgccg actggcgcgc ccggcgctcc tcctccggat cggccttctg
     3601 ccggggccgc ttcgtttcgg cctggcgggc ctcggcagcc gccgtgcggg cccgcttctc
     3661 ccgcgccgcg cggaacgcct cccagtcatc gaaggtccct tcgacatcgt cgatgcgggc
     3721 gttctcgagc gcccagaccc gggtcaccag gttgcggagg agcgcccggt cgtggctcac
     3781 caggatcacg gtcccctcat aggcctcgag gctctcttcc agcgactcga tggactcgac
     3841 gtcgaggtgg ttggtcggct cgtcgaagac cagcaggttg gcctccgcca gcgccatcat
     3901 cgcgagcgcc acgcgggcgc gctcccctcc cgacagcgat cccgccacgc gaaggaccga
     3961 atcgcccgag aagccgaacg tgcccaggtg cccctgcacc gccccgcggg tccacttggg
     4021 ccggaggtcg gcgatgatgt cgtagatggt cttgtcggcc ggcacctgcc ccaggtcctg
     4081 ctggtagtac gccaccacgg taccgtcgcc gacgcggacg gtgccgccct cggcgggacg
     4141 ctcgccgagg agggtcttga ggagcgtggt cttccccgcc ccgttcgcgc cgacgaagcc
     4201 gatcacgtcg ccccgcttga cccgggatga gaagcggtcg agcaggaccc ggtcgcccac
     4261 ggccaccttc acgtcctccg ccatcagcac ctggtcgccg ccgcggctgc cggcggtgag
     4321 cgtgaacggg ctcacccccg cctcgcccac gggcagcgag aggcggggga tgttgtccag
     4381 ccgc
//
LOCUS       DASUAP010000060         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_996161, whole genome shotgun sequence.
ACCESSION   DASUAP010000060 DASUAP010000000
VERSION     DASUAP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996161"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /gene="rsfS"
                     /locus_tag="VFU45_01475"
     CDS             <1..313
                     /gene="rsfS"
                     /locus_tag="VFU45_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU5357759.1"
                     /translation="AAVEGLKARDVVVLDLRGLTDATDYFIIASGTSDTHVRGIADSV
                     ARALGAVGQHPHHVEGLTAGRWALLDYVDFVVHLFHPEARAFYQLDRLWDDAPALRTG
                     S"
     gene            350..>2595
                     /locus_tag="VFU45_01480"
     CDS             350..>2595
                     /locus_tag="VFU45_01480"
                     /inference="COORDINATES: protein motif:HMM:NF016345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HEU5357760.1"
                     /translation="MRFTRRLLALLLLLPALPAAAAAQYFGQNQVQYSTFDFKIIETE
                     HFDVYYYDSERAAALDAARMAERSYARLSRILDHRFSERKPIILYASHSDFQQNNSVG
                     YSEGIEGVTDALKQRNVLPFPGSYKDFEHVLTHEMVHQFQYDTWSRGHSGGNLGALLT
                     LSPPGWFVEGMAEYLSIGPSDPATAMWLRDAALNGQLPTIDQLTNDPRIFPYRYGHAL
                     MAYIGERWGDEAIGTILKGTLSGSLEAAFRRVTGEDFKRLSTEWHDAIQKRFLPEIAD
                     RQKARAFAAEMLSQDKVGGTYHLAPSLSPDGRQVAFFSERNTFFVDLWLADAVTGKVI
                     RRLFKSTWSSNYETFRFIYSSVSWSPDGKYLAFVAKRGPRDVLIILDVAQNREVRAIP
                     VPLAAALTPSWSPDGKQLVFSGLDGGLSDLFIVNADGSGLRRLTSDKYADLEPVWSPD
                     GTSIAFVTDRGPETDFRTLTFGNYRIALYHLDSGTVEVLPHMDYGRNTSPQWSPDGHQ
                     LAFISDRTGVSDIYLYDLGERDLYRLTDLYTGAEGITPISPALSWAPQADRLAFVYYE
                     KGDFDVYSVLDPRAMKQQPFVDHPGENAMASLTLERPAALATTGVPAAPDTARAPVDS
                     AALRAQALTQGGSLYRSPHGFRPADIAPRHDSAAPAPMSVAAVLANDTISLPDTAEFT
                     LKRYHVKFTPDYVARPTVGYTRDNFGHGFYGGTTIVLSDILGNNSMIISGYLNGSLAE
                     ANVLFAYQ"
BASE COUNT          414 a          977 c          797 g          407 t
ORIGIN      
        1 cgcggcggtg gagggcctga aggcgcgcga cgtcgtggtg ctggacttgc gcgggctgac
       61 cgatgccacc gattacttca tcatcgcctc ggggacgtcg gacacccacg tgcgcggcat
      121 cgctgactcg gtcgcgcggg cgcttggcgc ggtggggcag cacccgcacc atgtcgaggg
      181 actgaccgca gggcgctggg ccctgctcga ttacgtggac ttcgtggtgc acctgttcca
      241 ccccgaggcg cgggcgttct accagctcga ccggctctgg gacgatgccc ccgcgctccg
      301 gacgggttca tagcggaccc ggttccatag atagaagagg gcttcgcaga tgcgcttcac
      361 ccgccggctc ctggcgctcc tcctcctcct ccccgccctc cccgcggccg cggccgcgca
      421 gtacttcggc cagaatcagg tccagtacag cacgttcgat ttcaagatca tcgagaccga
      481 gcacttcgac gtctactact acgacagcga gcgcgcggcg gcgctcgatg ccgcgcggat
      541 ggcggagcgc tcctacgccc ggctgtcgcg catcctggac catcgcttct ccgagcggaa
      601 gccgatcatc ctttacgcct cgcactccga tttccagcag aacaactcgg tcggctacag
      661 tgaaggcatc gagggcgtca ccgacgcgct caagcagcgg aacgtcctcc cgttcccggg
      721 atcgtacaag gacttcgagc acgtcctgac ccacgagatg gtccaccagt tccagtacga
      781 cacctggagc cgcgggcact ccgggggcaa cctcggcgcc ctgctgacgc tgagcccgcc
      841 cgggtggttc gtcgagggga tggcggagta cctctcgatc ggcccgtccg accccgccac
      901 cgcgatgtgg ctccgggacg ccgcgctcaa tgggcagctc cccacgatcg accagttgac
      961 caacgatccg cggatcttcc cgtatcggta cggccatgcg ctcatggcct acatcggcga
     1021 gcgctggggc gatgaggcca tcggcaccat cctcaagggg acgctctccg gctccctcga
     1081 ggcggccttc cgccgggtga ccggcgagga cttcaagcgc ctctccaccg aatggcacga
     1141 cgccatccag aagcgcttcc tccccgagat cgccgaccgg cagaaggcgc gcgccttcgc
     1201 cgccgagatg ctctcccagg acaaggtcgg gggcacctac cacctggcgc cgtcgctctc
     1261 gccggacggc cggcaggtcg cgttcttcag cgagcgcaac accttcttcg tggacctgtg
     1321 gctggccgac gccgtcaccg gcaaggtgat ccgccggctc ttcaagtcca cgtggagcag
     1381 taactacgag acgttccgct tcatctactc gtcggtgtcc tggtcgccgg acgggaagta
     1441 cctggccttc gtggccaagc gaggcccgcg cgacgtcctc atcatcctgg acgtggcgca
     1501 gaaccgcgaa gtccgcgcca tcccggtccc gctcgcggcc gcgctcacgc cgagctggag
     1561 ccctgacggg aagcagctgg tgttcagcgg cctcgacggc gggctctccg acctcttcat
     1621 cgtgaacgcc gacggctccg gccttcggcg cctcacgagc gacaagtacg ccgacctgga
     1681 accggtctgg tcccccgacg gcacgtcgat cgccttcgtc acggaccgcg gtccggagac
     1741 cgacttccgg acgctcacct tcggcaacta ccggatcgcg ctctaccacc tcgacagcgg
     1801 caccgtcgag gtcctgccgc acatggacta cggccggaac acgagcccgc agtggtcacc
     1861 ggatggccac cagctggcct tcatctccga ccgcaccggt gtgagcgaca tctacctcta
     1921 cgacctcggc gagcgggacc tctaccgcct caccgacctc tacaccgggg ccgaggggat
     1981 cacgccgatc tcaccggcgc tctcgtgggc gccccaggcc gaccgcctcg ccttcgtgta
     2041 ctacgagaag ggggacttcg acgtctacag cgtcctcgat ccccgggcga tgaagcagca
     2101 gccgttcgtc gaccaccccg gcgagaacgc gatggcgtca ctcacgctgg agcgccccgc
     2161 cgccctggcc accaccggcg tgcccgcggc ccccgacacc gcgcgggccc ccgtcgattc
     2221 ggccgccctg cgcgcgcagg cgctcaccca gggggggtcg ctctaccggt ccccgcacgg
     2281 attccgcccc gctgacatcg cgccccggca cgacagcgcg gcgcccgccc cgatgtcggt
     2341 ggcggcggtg ctggccaatg acacgatctc gcttccggac accgccgaat tcaccctcaa
     2401 gcggtaccac gtcaagttca cccccgatta cgtcgcccgg cccacggtag gctacacccg
     2461 cgacaacttc gggcacggct tctacggcgg cacgaccatc gtcctgagcg acatcctcgg
     2521 caacaactcg atgatcatct cgggctactt gaacggcagc ctggcggagg cgaacgtgct
     2581 cttcgcctac cagaa
//
LOCUS       DASUAP010000061         7366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1186971, whole genome shotgun sequence.
ACCESSION   DASUAP010000061 DASUAP010000000
VERSION     DASUAP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186971"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1176)
                     /gene="aroF"
                     /locus_tag="VFU45_01485"
     CDS             complement(136..1176)
                     /gene="aroF"
                     /locus_tag="VFU45_01485"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5357761.1"
                     /translation="MLVVMRHDAAQEDIQRVVSAIEELGYQARPMPGKQRTTVGLVGN
                     DGRVDSSRLAALPGVAEIIHVTQPYKQVSREWKAESTQVRLAGGVVVGGEDVVIMAGP
                     CSVETEAQILAAAHAVRDAGATILRAGAFKPRSSPYSFQGLGKPGLALLKRAREETGL
                     LIVTEAMDPEGADLVAEVADIIQVGARNMQNYSLLKRVGRLRKPVLLKRGLSATIQEL
                     LLSAEYILAEGNPDVILCERGIRSFDSQTRNLFDLSAVPVVKSLSHLPIIADPSHGTG
                     HREFVPPMARAAVAAGADGLLVEVHPAPDRALSDGAQSLYPDQFDRMMREVRQIAEAI
                     GRRVAEPAGLGA"
     gene            complement(1480..4944)
                     /locus_tag="VFU45_01490"
     CDS             complement(1480..4944)
                     /locus_tag="VFU45_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5357762.1"
                     /translation="MKLTRLELSGFKSFADTMSLDFEQGVTAIVGPNGCGKSNVSDAV
                     RWVLGEQSARLLRGGKMEDVIFQGSTGRRPGNLSEVSLYLDNSDGVLPVAYKEVVITR
                     RLSRSGQSEYLLNKANVRLRDIQDLLRGTGLGSDAGVVIEAKMIDLLLSDRAEERRAL
                     FEEAAGIGLYRDRKQSTERRLEETAVDLQRVEDLIAEVQTQLRSLARQRGKAERHSKL
                     QEEKFSVQVTLAHRQLARIEDLLGTFGDRRQELATALPEARERLAAAERRRDEAERAR
                     TSVDAERTEIVRRLGAVRVELGRLDGDLALAAERLANAAQRRVRAADEREMMLERIRG
                     AAREREAASAEREAALLEHARIQGELTGRSATEQGVRDRLTAERTALRKLEEELQQRA
                     HTLRALEGERAALDLDLAAVREQEAQAQARHDALTAELAVAESRRQDAAETAERQAQV
                     ARQAGATAEQARHDVAEAKRREALERAARRQAEEALAQARARRQALEELERDRVGLAP
                     GAAALLAARGRFGDAILGPLSDFITTSQRDADLAERLLGEWVHAVLVRDRAAVSAIQA
                     WHQETQPGAVVVLPLHPGPTGAAGRSVGHGLQVAEPAVAWVHALVSGSEVLDPTGRVL
                     QRPNGAILLTGAAPSGPLRRRADLESLGVEVAAAKQKLAAADATLAATVQRLAELEAA
                     LEAAAAATEQAREGERAAAAVREDAARVAANLAREITESERQAASLKERLERGERRLA
                     EVDAAHTDGELARVKQDEALSSAREALHGLEGQQEGAREARVRWQVLEAQVSARLEAA
                     AERVARAERTAAEAEAQAGALGAEVAQLALDAEAVEGQRAQWLEQRAERRVAALELEA
                     AAADVEATAADAEQELVEADAALSAGRTAVESMTEEHHRMEVDATDLIARRRAIVERI
                     EADWRKPLDALLADAVLLDLDLETLEAENARIVEALEGIGPVNALAVEEYGEEAKRLE
                     FLTAQRDDLVAGRLSLQQAIREIDGTAKAMFVETFDAVRENFKRVFQTLFGGGECELR
                     LASPDEPLESEIDIHAAPRGKRTQRIHLLSSGERTLVATSLLFSIYLTKPSPFCLMDE
                     VDAPLDDANVGRFTRLLDEFKSATQFIVITHNPRTMQSADAVYGVTMQEPGVSTIVGV
                     RLGETAGA"
     gene            complement(4967..6355)
                     /locus_tag="VFU45_01495"
     CDS             complement(4967..6355)
                     /locus_tag="VFU45_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU5357763.1"
                     /translation="MPAPSRIIPGLILLAGAACRSVPSRGPLYAATAQPPQAVSLLRV
                     PLKGGKPALLRLPTLTPSSWVGREDLPAPRALVGTDIDARLVYVRDLKGNVVALDLEA
                     GRNHPVLSNVAAATVGPDGTLYTVGTDHVVSETADRTPVTFPCQLSAGARELFAGGDE
                     SALALLEGPAPALAIVDRQHPPTLVSIPSGEAAATTWGNLVAIAADSAVVLYTPGSAH
                     PFRSIDAGDHVREVAFSPSGHRLYVGRAGSDALGVFDRFSWTRLSDIDLPGDPAALRP
                     DPFGEYLLVRPARGDSVWVVDLSTNELLGSWPASWGADLPTVAKSRYLLVRQGQDIVS
                     YDLATAKLPETGRVPGGAADLWLVLQWAPRGEIPLGPEIDSTALAAADSAPDTRIYVQ
                     VSSSQNPAWANELARKMKDVGLPAQVIKPTHEDEGYRVVLGPYPTREAAESTGKKLGQ
                     PYFIYTPDAAAQ"
     gene            complement(6441..>7366)
                     /locus_tag="VFU45_01500"
     CDS             complement(6441..>7366)
                     /locus_tag="VFU45_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5357764.1"
                     /translation="GHRINYGVGLSTAPYYLIGAGSNFNNDTVTTSVDRFIVRQAFGQ
                     AFYPFNRFRRLEGQLALTNIQEDSLKILDDLLSGTEKRDTKYLGSAYFAQPSLALVFD
                     NSIPGYVGPYIGRRSRFEVSQTLGDWHITQLAADYRRYDHLVGPFVLATRAMVVDRSG
                     RDAGRQLLFLGYPDYIRGYTSGSFYSNECVNVDQGGYTGCKGLDNLLGTSIGVANAEL
                     RFPLLNASLGFVPIGFPPIEGAIFYDVGKAWGSPDGSLHAAPIANGDLVSSWGTSIRV
                     NLLGFVVLRFDYAFPTRPVKGYWTVSLGPTF"
BASE COUNT          975 a         2569 c         2746 g         1076 t
ORIGIN      
        1 agtcggcctg gaacgccgca agcgcgccgt acgcagcggc ggcgcgctgg accacggcaa
       61 cgccgtcctg ggcggcgagc ggaagggcgg ccgccgcgag gaggagcgcg gcgagcggcg
      121 ccacccggcg ggcagtcacg cgccgagtcc ggcgggctcc gccacccgtc ggccgatcgc
      181 ctcggcgatc tgccggactt cgcgcatcat ccggtcgaac tggtcggggt agaggctctg
      241 ggcaccgtca gagagggccc ggtcgggggc gggatgcacc tcgaccagca ggccgtcggc
      301 gccggccgcc accgcagcgc gcgccatcgg cgggacgaac tcccggtggc cggtgccatg
      361 gctcgggtcg gcgatgatcg ggaggtggga gagcgacttg accaccggga ccgccgaaag
      421 gtcgaagagg ttccgggtct ggctgtcgaa gctccggatg ccgcgctcgc acaggatgac
      481 gtccggattc ccctcggcga ggatgtactc ggccgagagg aggagctcct ggatcgtggc
      541 ggagagtccc cgcttgagga ggaccggctt cctgagccgg ccgacgcgct tgagcaggga
      601 atagttctgc atgttccggg cgccgacctg gatgatgtcg gccacctcgg ccacgaggtc
      661 agcgccttcg gggtccatcg cctcggtgac gatgaggagc ccggtctcct cccgcgcccg
      721 cttcaggagc gccagcccgg gcttgcccag gccctggaat gagtagggcg agctgcgggg
      781 cttgaaggcg ccggcccgga ggatcgtggc gcctgcgtcg cggaccgcgt gcgcggcggc
      841 caggatctgg gcctcggtct cgaccgagca ggggcccgcc atgatgacca cgtcctcgcc
      901 cccgaccacc acgcccccgg cgagccggac ctgggtggac tccgccttcc actcgcggga
      961 cacctgcttg tacggctggg tgacgtggat gatctcggcc acaccgggca gcgccgccag
     1021 gcgtgagctg tccacccggc cgtcgttgcc gaccaggccg acggtggtgc gctgcttccc
     1081 cggcatcggg cgggcctggt agcccagctc ctcgatggcg gagacgaccc gctggatatc
     1141 ttcctgggcg gcatcgtgcc gcatgacgac gagcatcagg tcctcggacg aaagaccccg
     1201 gggcatcgga ccccggggag gtgcctgcct cggtgaacgg taaacgggga acggtgaacg
     1261 ggccactccg gcggggtcgc gggtcgaggg tcgcgagtcg cgggtcgcga gtcgcaggtc
     1321 gaagggcaca tcgcccgctc ccacacgtcc catcccccac ctcttacctc tcacctctta
     1381 cctcttacct ctcacctctt acctctcacc tctcacctct tacctctcac ctcttacctc
     1441 tcacctctca cctcttacct ctcaccttcc acccgcccct cacgcccccg ccgtctcccc
     1501 cagccgcacg ccgacgatcg tcgagacccc cggttcctgc atcgtgacgc cgtagaccgc
     1561 gtcggccgac tgcatcgtgc gcgggttgtg ggtgatgacg atgaactggg tggccgactt
     1621 gaactcgtcg agcagccgcg tgaagcggcc cacgttggcg tcgtcgagcg gtgcatccac
     1681 ctcgtccatg aggcagaacg ggctcggctt ggtgaggtaa atcgaaaaga ggagcgaggt
     1741 ggccaccagc gtccgctcac ccgaggagag caggtggatc cgctgggtcc gcttgccgcg
     1801 gggggcggcg tggatgtcga tctcgctctc cagcggctcg tcggggctgg ccaggcgcag
     1861 ctcgcactcg ccaccgccga agagggtctg gaacacccgc ttgaaattct cccgcaccgc
     1921 gtcgaacgtc tcgacgaaca tcgccttggc ggtaccgtcg atctcgcgga tggcctgctg
     1981 cagcgagagc cgcccggcca cgaggtcgtc ccgctgggcg gtcaggaact ccaggcgctt
     2041 tgcctcctcc ccgtactcct cgaccgccag cgcgttgacc gggccgatcc cctcgagcgc
     2101 ctcgacgatg cgggcgttct ccgcctcgag cgtctcaagg tcaaggtcga ggagcaccgc
     2161 gtcggccagc agggcgtcga gcggcttgcg ccagtcggcc tcgatgcgct cgacgatggc
     2221 ccggcgccgg gcgatgagat cggtggcgtc cacctccatc cggtggtgct cctcggtcat
     2281 cgactcgacg gccgtgcggc cggcgctgag cgcagcatcg gcctcgacca actcctgttc
     2341 cgcgtcggcc gcggtcgcct cgacatcggc ggcggcagcc tcgagctcca gcgcggccac
     2401 gcggcgctcg gcgcgctgtt cgagccactg cgcccgctgc ccctcgacgg cctcggcgtc
     2461 gagcgccagc tgcgcgacct ccgcgcccag ggcgccggcc tgcgcctccg cctcggcggc
     2521 ggtgcgctcc gcccgtgcca cgcgctccgc cgccgcctcc agccgggcgg acacctgcgc
     2581 ctcgagcacc tgccagcgga cccgcgcctc ccgcgcgccc tcctgctggc cctcgagccc
     2641 gtgcagtgcc tcgcgggcgg agctcagcgc ctcgtcctgc ttgacgcggg ccagctcgcc
     2701 gtcggtgtgc gccgcgtcga cttcggccag ccggcgctcg ccccgctcga ggcgttcctt
     2761 gaggcttgcc gcctggcgct ccgattcggt gatctcccgg gccaggttgg ccgcgacccg
     2821 cgcggcgtcc tcccgcacag cggcggcggc gcgctccccc tcgcgggcct gttcggtggc
     2881 ggcggcagcg gcctcaagcg cggcctcgag ttcggccagg cgctggacgg tggccgccag
     2941 ggtggcgtcc gccgcggcga gcttctgctt ggcggcggcg acttcgacgc cgaggctttc
     3001 gaggtccgcg cggcggcgga gcgggccgga aggcgccgcc ccggtgagca ggatggcgcc
     3061 gttgggccgc tggaggaccc ggccggtcgg gtcgagcact tccgatcccg agaccagtgc
     3121 gtggacccag gccacggcgg gctcggccac ctgcaggccg tggcccaccg accgtccggc
     3181 ggcaccggtc gggcccggat ggagcgggag caccacgacc gccccgggct gggtctcctg
     3241 gtgccaggcc tggatggcgc tcacggcggc gcggtcccgg accaggaccg cgtggaccca
     3301 ttctcccagg aggcgctcgg ccaggtcggc gtcgcgctgg gaggtggtga tgaagtcgct
     3361 gagcgggccg aggatggcgt cgccgaagcg gccgcgggcc gcgaggaggg cggcggcgcc
     3421 gggggccagg cccacgcgat cccgttccaa ctcctcgagc gcctggcgcc gggcgcgcgc
     3481 ctgggcgagc gcctcctccg cctggcggcg ggccgcccgt tccagcgcct cgcggcgctt
     3541 cgcctcggcc acgtcgtggc gcgcctgttc ggcggtggcg cccgcctggc gggccacctg
     3601 ggcctggcgc tcggccgtct cggcggcgtc ctggcgccgg ctctccgcca cggcgagctc
     3661 ggcggtcagg gcatcgtgcc gcgcctgggc ctgcgcctcc tgctcccgca ccgcggcgag
     3721 gtcgaggtcg agcgcggcgc gttcaccttc gagggcccgg agcgtgtgcg cccgttgctg
     3781 gagctcctcc tcgagcttgc ggagcgcggt gcgctcggcg gtgagccggt cgcgcacccc
     3841 ctgctcggtg gcgctccggc cggtgagctc gccctggatc cgggcgtgct ccaggagggc
     3901 ggcctcccgt tcagcggacg cggcctcgcg ttcgcgggcg gcgccgcgga tgcgttcgag
     3961 catcatctcc cgctcatcgg cggcgcgcac cctgcgctgg gcggcgttgg ccaggcgctc
     4021 tgccgccaag gccagatcgc cgtcgaggcg gcccagctcg acccggacag cgcccagccg
     4081 ccggacgatc tcggtccgct cggcgtcgac cgacgtgcgg gcccgttccg cctcatcccg
     4141 gcgccgttcg gcggcggcca ggcgctcgcg ggcctcgggc agcgcagtgg cgagttcctg
     4201 gcgccggtcg ccgaaggtgc cgaggagatc ctcgatccgg gccaactgac ggtgcgccag
     4261 ggtcacctgg accgagaact tctcctcctg gagcttggag tgtcgttccg ccttcccgcg
     4321 ctggcgggcc aggctccgga gctgggtctg cacctccgcg atcaggtcct ccacccgctg
     4381 gaggtcgacc gcggtctcct ccagccggcg ttcggtgctc tgcttgcggt cgcggtagag
     4441 gccgatcccc gccgcctcct cgaagagggc ccggcgctcc tccgcgcggt cggagagcag
     4501 gaggtcgatc atcttggcct cgatcacgac gccggcgtcg ctgccgagcc cggtgccgcg
     4561 gaggaggtcc tggatgtcgc ggaggcggac gttcgccttg ttgaggaggt actcgctctg
     4621 gcccgaccgg gagagccggc gggtgatcac gacctccttg tacgccaccg ggaggacgcc
     4681 gtcggagttg tcgaggtaga gcgacacctc cgagagattg ccgggacgcc gtccggtgga
     4741 tccctggaag atcacgtcct ccatcttgcc gccgcgcagg aggcgcgccg actgctcgcc
     4801 cagcacccag cgcaccgcgt ccgagacgtt cgacttgccg cagccgttgg ggccgacgat
     4861 ggcggtgacg ccctgttcga agtcgagcga catcgtgtcg gcgaacgact tgaagccgga
     4921 gagctcgagc cgggtgagct tcatcgcagg tccggggtgg aggggctcat tgggcggcgg
     4981 cgtccggggt atagatgaaa tagggctggc cgagcttctt gccggtggat tcggcggcct
     5041 cacgggtcgg gtaggggccg agcaccacgc ggtagccttc gtcctcgtgg gtgggcttga
     5101 tgacctgggc ggggaggccc acgtccttca tcttgcgcgc cagttcattg gcccacgccg
     5161 ggttctgcga actgctcacc tggacgtaga tccgggtgtc gggggcgctg tcggcggccg
     5221 cgagcgccgt ggagtcgatc tcgggcccga gcgggatctc gccgcggggc gcccactgca
     5281 gcaccagcca gaggtcggct gcgccgccgg ggacgcggcc ggtctccggg agcttggcgg
     5341 tggccaggtc gtagctgacg atgtcctggc cctggcgcac cagcaggtag cgcgacttgg
     5401 ccaccgtggg caggtccgcg ccccagctcg cgggccagct gccaaggagc tcgttggtgg
     5461 agaggtcgac gacccagacc gaatcgcccc gggcgggacg caccaggaga tactcgccga
     5521 acgggtcggg acggagggcg gccgggtcgc cggggaggtc gatgtcggag agccgggtcc
     5581 aggaaaagcg gtcgaacacg ccgagcgcgt ccgagcccgc ccgcccgacg taaagccggt
     5641 gcccgctcgg cgagaacgcc acctctcgga cgtggtcgcc ggcgtcgatg gaccggaatg
     5701 gatgggccga gccgggggtg tagaggacca ctgcgctgtc cgcggcgatc gcgaccaggt
     5761 tgccccaggt cgtcgcggcc gcctcgccgg aagggatcga cacgagcgtg ggcggatgct
     5821 ggcggtccac gatggcaagc gcgggggcgg gtccctcgag gagggccagc gccgattcgt
     5881 ccccgccggc gaagagttct cgcgcgcccg cggagagctg gcacggaaag gtgacggggg
     5941 tccggtcggc cgtctcgctc accacgtggt cggtgcccac ggtgtagagc gtgccgtcgg
     6001 ggcccacggt ggccgcggcc acgttggaga ggaccgggtg gttccgcccc gcctcgaggt
     6061 cgagcgccac gacgttgccc ttgaggtcgc gcacgtacac cagccgggca tcgatgtcgg
     6121 tcccgaccag cgccctgggc gccgggaggt cctcccgacc cacccagctc gacggtgtga
     6181 gggtcgggag gcggaggagc gcaggcttcc cgcccttgag cgggacgcgg agcagtgaga
     6241 cggcctgggg cggctgggcg gtggcggcgt agaggggccc gcgcgacggc acggaacggc
     6301 aggcagcgcc cgccaggagg atcaggcccg gaatgatgcg ggaaggggct ggcacggcct
     6361 caagataatg aaaggcgggg cagtgggtca gtgggggagt gggtcagtgg ccgctgccgc
     6421 actgacccgc tgacccgctc ctagaacgtc ggtccgaggc tcaccgtcca gtatcccttg
     6481 actggacgcg tggggaaggc gtagtcgaac cggagcacca cgaagcccag caggttgacg
     6541 cggatcgagg tgccccacga ggagacgaga tcgccgttgg cgatgggcgc ggcgtggagc
     6601 gacccatcgg ggctgcccca cgccttgccg acgtcgtaaa agatggcgcc ctcgatcggg
     6661 gggaacccga tcggcacgaa cccgagcgag gcgttgagga gcgggaagcg gagttccgcg
     6721 ttcgccacgc cgatcgaggt gccgaggagg ttgtcgagcc ccttgcaccc ggtgtagccg
     6781 ccctggtcga cgttgacgca ctcgttgctg tagaatgagc cggacgtgta gccgcggata
     6841 tagtcgggat agccgaggaa gaggagctgg cgcccggcgt cgcggccgga gcggtccacc
     6901 accatggcgc gggtggcgag cacgaagggg cccaccaggt ggtcgtagcg gcggtagtcg
     6961 gccgcgagct gggtgatgtg ccagtcgccg agcgtctggg agacctcgaa gcgtgaccgc
     7021 cggccgatgt agggccccac gtatcccggg atcgagttgt cgaagaccag cgccagggac
     7081 ggctgggcga agtaggcgga accgaggtac ttggtgtcgc gtttctcggt cccgctcaac
     7141 aggtcatcga ggatcttgag agagtcctcc tggatgttcg tgagcgcgag ctggccctcg
     7201 aggcggcgga accggttgaa cgggtagaac gcctgcccga acgcctgccg cacgatgaag
     7261 cggtcgaccg acgtggtcac cgtgtcattg ttgaagttgc tgcccgcgcc gatcaggtaa
     7321 tagggcgccg tcgagaggcc gacgccgtag ttgatgcggt ggcccg
//
LOCUS       DASUAP010000062         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_28657, whole genome shotgun sequence.
ACCESSION   DASUAP010000062 DASUAP010000000
VERSION     DASUAP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28657"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..434)
                     /gene="rpsH"
                     /locus_tag="VFU45_01505"
     CDS             complement(39..434)
                     /gene="rpsH"
                     /locus_tag="VFU45_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU5357765.1"
                     /translation="MSLTDPVADMLTRIRNACAAGHRRVDMPVSKLKGELARLLKDNH
                     YIADFKILDDGAHGVLRLYLKYHDEAPVIRELRRVSTPGLRRYVKSREIPRVKNGLGM
                     AIVSTSRGLMSDRDARTQNLGGELLAVVW"
     gene            complement(447..632)
                     /locus_tag="VFU45_01510"
     CDS             complement(447..632)
                     /locus_tag="VFU45_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEU5357766.1"
                     /translation="MAKTCWIERAKRTPKFKSRVVRRCQRCGRSRAVLRKFGLCRICF
                     RDLALQGMIPGVRKASW"
     gene            complement(679..1239)
                     /gene="rplE"
                     /locus_tag="VFU45_01515"
     CDS             complement(679..1239)
                     /gene="rplE"
                     /locus_tag="VFU45_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU5357767.1"
                     /translation="MPRLRQRYQDTVRARLKQEFALGNVHEIPTVVKVVLNVGMGEAS
                     KNPKQLEQALEELALITGQRPVVTRAKKAIANFGLREGMPVGCTVTLRGARMWEFLDR
                     FITLAVPRVRDFRGLPSRSFDGRGNYTFGVKEQMIFPEINFDKVEKVHGMDITIVTST
                     TRDDLAMALLRELGWPFRGEQPPAAG"
     gene            complement(1345..1725)
                     /gene="rplX"
                     /locus_tag="VFU45_01520"
     CDS             complement(1345..1725)
                     /gene="rplX"
                     /locus_tag="VFU45_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU5357768.1"
                     /translation="MKPLVYRKAKRVRQAPVVRQKLHITKGDTVQVISGDDKGKRGKV
                     LRVHPKSGRLTIEGVNVTTKHVRATQQAEGGRVKREAPIHHSKAMLIDPKTNAPTRVR
                     RQKDSDGTVERVGVKSGVAIPRSR"
     gene            complement(1727..2095)
                     /gene="rplN"
                     /locus_tag="VFU45_01525"
     CDS             complement(1727..2095)
                     /gene="rplN"
                     /locus_tag="VFU45_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU5357769.1"
                     /translation="MIQQETIVRIADNSGARKALVIRVLGGSRRRYAGLGDKVVVAIK
                     DAIPTGQVKKGDVAKAVIVRTAKETRRKDGSYIRFDENAAVLINEQGEPRATRIFGPV
                     ARELRDKRYMKIVSLAPEVL"
BASE COUNT          276 a          811 c          671 g          403 t
ORIGIN      
        1 ccacgggcat cttgccgatg cggctcacgg tcgcctcctc accacacgac ggcgagcagc
       61 tcgccgccga ggttctgggt gcgcgcgtcg cggtcgctca tgagccctcg cgacgtgctc
      121 acgatggcca tgccgaggcc gttcttcacg cgcgggatct cgcggctctt cacgtagcgc
      181 cgcaggccgg gcgtggagac gcggcgcagc tcgcggatga ccggcgcctc gtcgtggtac
      241 ttgaggtaca gccggagcac gccgtgcgcg ccgtcatcga ggatcttgaa atccgcgatg
      301 tagtggttgt ccttcagcag ccgcgcgagc tcgcccttga gcttgctcac cggcatgtcc
      361 acgcgccggt ggcccgccgc gcaggcgttg cggatgcggg tcagcatgtc cgctacggga
      421 tctgtcaggc tcatcgcacc cttcctctac cagctggcct tgcgcacgcc ggggatcatc
      481 ccctgcagcg cgagatcgcg gaagcagatt cggcacaggc cgaacttgcg cagcaccgcg
      541 cgcgagcggc cgcaccgctg gcagcggcgc accacccggg acttgaactt cggcgtgcgc
      601 ttcgcgcgct cgatccaaca cgtcttcgcc ataaaactcc ctgttcgggc agagccgtgg
      661 ctctgcccct gtcgtccgtt accctgccgc gggcggctgt tcgccgcgga acggccagcc
      721 cagctcccgg aggagcgcca tggccaggtc gtcccgcgtg gtgctcgtca cgatcgtgat
      781 gtccatcccg tggaccttct cgaccttgtc gaagttgatc tccgggaaga tcatctgctc
      841 cttgacgccg aaggtgtagt tcccgcggcc gtcgaagctc cggctcggca gcccgcggaa
      901 atcgcgaacc cgcggcacgg caagggtgat gaaccggtcg aggaactccc acatgcgggc
      961 gccccggagc gtcaccgtgc agcccaccgg catcccttca cggaggccga agttggcgat
     1021 cgccttcttg gcccgcgtca ccaccggccg ctggccggtg atgagcgcca gctcctccag
     1081 tgcctgctcc agctgcttcg ggttcttgga cgcctcgccc atcccgacgt tcagcaccac
     1141 cttcaccacg gtcggaatct cgtgcacgtt gccgagggcg aattcctgct tcagccgcgc
     1201 ccgcaccgtg tcctggtacc gctgccggag ccgcggcacg ccggcgctcg cctgcggcgc
     1261 cgactccacc gcatagctcg ccttggcctt gtccttcccg ccgcgcttcg gcgccttgac
     1321 ctgttccgct gtgtccgcca tcgcttaccg gctcctcggg atcgcaacac ccgacttgac
     1381 gcccacccgc tccaccgtgc cgtcgctgtc cttctgccgc cgcacccggg tcggggcatt
     1441 ggtcttcggg tcgatcagca tggccttcga gtgatggatc ggcgcctcgc gcttgacccg
     1501 ccctccctcg gcctgctggg tggcccgcac gtgcttcgtg gtcacgttca ccccttcaat
     1561 ggtgagccgg cccgacttgg ggtgcacgcg gagcaccttc ccccgcttcc ccttgtcgtc
     1621 gcccgagatc acctgcacgg tgtcgccctt ggtgatgtgc agcttctgcc gcaccaccgg
     1681 ggcctggcgc acccgctttg ccttccggta taccagcggc ttcatgtcag agtacctccg
     1741 gcgcgagcga cacgatcttc atgtaccgct tgtcgcgcag ctcgcgcgcc accgggccga
     1801 agatgcgggt cgcgcgcggc tccccctgct cgttgatcag caccgccgcg ttctcgtcga
     1861 agcggatgta ggagccgtcc ttgcggcggg tctccttcgc ggtgcggacg atgaccgcct
     1921 tggccacgtc ccccttcttc acctggccgg tcgggatggc gtccttgatg gcgaccacca
     1981 ccttgtcgcc caggccggcg taccgccggc ggctcccgcc caggacccgg atgaccagcg
     2041 ccttgcgggc gccggagttg tccgcgatcc ggacgatggt ttcctgttgg atcatttggc
     2101 ccgctccacg atctcgatca cccgccagcg caccgtcttc gcgagcggcc gcgtctccat
     2161 g
//
LOCUS       DASUAP010000063         7866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_165084, whole genome shotgun sequence.
ACCESSION   DASUAP010000063 DASUAP010000000
VERSION     DASUAP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165084"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="VFU45_01530"
     CDS             <1..1668
                     /locus_tag="VFU45_01530"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEU5357770.1"
                     /translation="LDGEQWVGQRGLLAVGVTALVLALAYLLKLSFDRGWVSPAVRCT
                     GGALVGVLVGGIGWRLERRGFRSYGAALVGAGAGMTYLAVWAAGRLYGFLPPPSAIAA
                     LALVSLGLALLAYAIDVEALGGTAAVGAFLAPVAIGPSSSNPNLLLIYLGAMGAALGT
                     VAALKRWRTAAILVLLAYFGLGSWAAHEAYPWLSLVFAVVGGAGGIWLGLRERWWETR
                     LVAFGGSWGCLAAAAPGLGPDGLLLAGGAALSLPVWRHGLAHPVVPFAGRDGTGASWR
                     ESSYFYATPWLLGWAAGRIQLPAFESHPGLAAACVAVAYLAAAFGAAGRPFALVAAGA
                     AGVGALCEWSGLHAAWALGALALLWGAVGRGVRRPDWERYGGVTLLAAVTQLALIARD
                     QRAPGAPAFVDSWALTLWALTVLAVAWSAGLVGGPTDDPVLRRIRRAGWLVGGAMIFV
                     GVTGELQRFFALADLSRETRALAGGLAVSAWWIVFAAGLILLGFRGSLKPVRVAGLWV
                     SGLAVGKVLLVDLSALDALYRIGSVLLLGLGSLGAAYLYHRRARAAG"
     gene            1759..3465
                     /locus_tag="VFU45_01535"
     CDS             1759..3465
                     /locus_tag="VFU45_01535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEU5357771.1"
                     /translation="MPSIVTPDTFAQAGWAGVRPWYEELEARPLGAGSLEAWLADWSR
                     LESALGEAAARAMIAYTCDTDNAAKLDAHLRFSSDILPKAEELSVRLARRFVAFGVVP
                     PGLEQPVARFRRAIEVFREANVPLFTETERLGSEYQQITGGLTAAWGDETLPLPRLAP
                     FLKSIDRAERERAFRAWAAPYIGRRAEFAALFDRMYVARQEVARNAGFADFQAYSFAA
                     KCRFDYTPDDCRRFHDSVEQAVMPAVERIHARRRARLKLRALRPWDTNVDLWGRPAIR
                     PFSSEAEFIATAERVFQRVDPALGREFATMREERLLDLTSRTGKAPGGYCETLSASGR
                     PFIFMNAAGTVEDVMTLLHESGHAFHAFASHPLPYLWQRHPTMEMAELASMSMELLAF
                     SALRKADGGFFSDAEHRRAKREHLEDALLTLAHVASVDAFQSWIYTSGAGGDAAARDQ
                     AWLRIRGRFERGIDWSGLDAERVARWYRQLHIFLYPFYYIEYGLAGLGALQLWRNSLA
                     DHPGALAAYRRALALGGTRSLPELYRAAGARLVFDAAGMAELVAVVEEELGRLDEGEP
                     GR"
     gene            3519..3737
                     /gene="infA"
                     /locus_tag="VFU45_01540"
     CDS             3519..3737
                     /gene="infA"
                     /locus_tag="VFU45_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU5357772.1"
                     /translation="MAKEEGIEMEGVVTEVLPDRNYRVLLENGHSVLAYAAGKMSKFK
                     IRVLEGDRVSVVLSPYDLTRGRVIYRHK"
     gene            complement(3841..4053)
                     /locus_tag="VFU45_01545"
     CDS             complement(3841..4053)
                     /locus_tag="VFU45_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU5357773.1"
                     /translation="MARMQGTVKWFNDAKGFGFITPENGEKDCFVHHTAIKADGFRSL
                     AEGEKVEFDMVMGAKGPAAENVTKVK"
     gene            complement(4292..6064)
                     /locus_tag="VFU45_01550"
     CDS             complement(4292..6064)
                     /locus_tag="VFU45_01550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5357774.1"
                     /translation="MATTPVTSTPPPLATHDRVKAAVGARYRLDTLVASSRERVLYAA
                     YDTVLNRPVSLRVNFYTDEAIRRWFLREAEALGQLDHPAIRHAYDAGVAGDVAFRVGN
                     WIEGESLQEAVQRGPRPIPAVHSLARDLLSALEHAHAHGIIARRIVPAAVLLNLAGRG
                     VVTELRFSSWTLPMIPPDERPSGLPFMAPEVRDGQVGDQASDVYTVGAILYYAITGVE
                     PPLDCTTLKRPMELRPATPRAIERILLRALRPNPADRYLTASEMLEDFASDAGTFDNP
                     ALMVPAARTGATGFEDTGLYEKRLRRALGDDYELLSLLGAGGFGRVYRVRDLHLEREV
                     ALKVMHPHLTADPAVVERFRREAQLAARLNHPNIVDIYDIAGRSGLLWYTMELVDGPN
                     VAQKVERDGPLPVDDLMRLLREALSALGHAHQQGLVHRDVKPENLLLERDGRLRITDF
                     GLALALRGQGKFGGATSQSGTPQFASPEQLLGERVDQRTDLYSLGAVAYFALLGEPPF
                     PGSTPEQVLARQTANRFPELQRRRPDVRLQLVEVLERALQSEAELRYPSAAEFQQALN
                     RVTGLRHRSGEWTKQALRWWKSRP"
     gene            complement(6309..6908)
                     /locus_tag="VFU45_01555"
     CDS             complement(6309..6908)
                     /locus_tag="VFU45_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357775.1"
                     /translation="MTGYSDRTHHALAFAAKHLSRDATRPAAALARPANLAVILASYG
                     CDEVTVVAAVLHEVIAQSRAQERADLIRRLGEKFGPVALAVALEDQPQDPPASRGLRT
                     TWETECVDRLARLAMLEPRTLSLRAAQEILDCGSILSDVRRLGREYLPNSRAVSGPQL
                     VWWFRSVAETLDRHDAWPRREMLGELRLLTRDLASELGA"
     gene            complement(6905..7567)
                     /gene="queE"
                     /locus_tag="VFU45_01560"
     CDS             complement(6905..7567)
                     /gene="queE"
                     /locus_tag="VFU45_01560"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEU5357776.1"
                     /translation="MPYAVKELFYTLQGEGAQAGTPAVFCRFAGCNLWSGREEDRAGA
                     VCTFCDTQFVGTDGPGGGKFASAEALARRAAEIWDDGDPARRAARTSPGSALVVCTGG
                     EPLLQLDRALIDAFHAAGFRVAVETNGTQPAPPGIDWICVSPKSGAPLVLTRGDELKL
                     VFPQPGGGPERYGSLDFDHFFLQPMDGPDRDRNTALALRYCLDHPRWRLSLQTHKLLG
                     IP"
     gene            complement(7674..>7866)
                     /locus_tag="VFU45_01565"
     CDS             complement(7674..>7866)
                     /locus_tag="VFU45_01565"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEU5357777.1"
                     /translation="TLTKREIIARGRALGVDYAATLTCYDPTPDGRACGRCDACRLRL
                     RGFAENGIEDPAVYVERER"
BASE COUNT         1021 a         2629 c         2938 g         1278 t
ORIGIN      
        1 ctcgatggcg agcagtgggt cggccaacgc gggttgctcg cggtgggcgt cacggcgctc
       61 gtgctggcgc ttgcgtacct cctcaagctc tcattcgatc gcgggtgggt atccccggcg
      121 gtacgctgta ccggcggcgc gctcgtcggc gtcctcgtcg gcggcatcgg ctggcgcctg
      181 gagcgtcggg ggttccggtc ctacggcgca gcgctcgtcg gggcaggggc agggatgacc
      241 tacctcgccg tctgggcggc ggggcggctc tacgggttcc tgccgcctcc gagcgccatc
      301 gccgcgctcg cgctcgtgtc tctcgggctg gccctcctgg cctacgccat cgacgtcgag
      361 gccctcggcg gcacggccgc cgtgggcgcc ttcctggcgc cggtcgcgat cggccccagt
      421 tcgagcaatc ccaaccttct cctgatctat ctcggcgcga tgggtgcggc cctcgggacg
      481 gtcgccgcgc tcaagcggtg gcgcaccgcg gccattctcg tcctgctcgc gtacttcggg
      541 ctcggcagct gggcggcgca cgaggcctat ccgtggctgt ccctggtctt cgcggtcgtc
      601 gggggcgccg gtgggatctg gctcggattg cgcgaacggt ggtgggagac ccgcctggtc
      661 gccttcgggg gaagctgggg ctgcctggcc gccgcggcgc cgggtctcgg accggacggc
      721 ctgctgctgg ccggtggggc agcgctctcg ctcccggtgt ggcgccacgg gcttgcgcat
      781 ccggtggtgc cctttgccgg gcgcgacggg acgggcgcgt cgtggcgcga atcatcctac
      841 ttctacgcca cgccctggct cctgggatgg gccgccggcc ggatccaatt accggcgttc
      901 gagtcccacc ccggcctcgc cgccgcgtgc gttgcggtcg cgtacctggc ggccgcgttc
      961 ggcgcggccg gaaggccgtt cgcgctggtg gcggccggcg ccgcgggggt cggcgcgctg
     1021 tgcgagtgga gcggccttca cgcggcctgg gcgctggggg cgctggcgct cctctggggg
     1081 gccgtcgggc gtggggttcg gcggccggat tgggagcgct acggtggcgt gacgctgctc
     1141 gcggccgtga cccagctcgc gctcatcgcg cgcgaccagc gggcacccgg agcgccggct
     1201 ttcgtggaca gctgggcgct cacactgtgg gccctgacgg tcctggcggt ggcgtggagc
     1261 gcggggctgg tcggcggacc gaccgatgat cccgtcctgc gccggatccg ccgtgccggc
     1321 tggctggtcg ggggcgccat gatctttgtc ggcgtgaccg gcgagttgca gcgcttcttc
     1381 gcactcgccg acctgtcgcg tgagacccgg gcgctcgccg gtgggctcgc cgtgagcgcc
     1441 tggtggatcg tgttcgcggc cggcctcatc ctccttggct tccgcggctc gctcaagccg
     1501 gtgcgggtgg ccgggctctg ggtctcaggt ctggccgtcg ggaaggtgct gttggtggac
     1561 ctgagtgcgc tcgacgcgct ctaccggatc ggctcggtcc tgttgctcgg gctgggctcg
     1621 cttggggccg cctatcttta ccatcgccgg gcccgggccg cgggttgacg ctcgcttgat
     1681 gccgcccggt gacattggcc gtcccgcccg tcttgcgcgg ccccatcaca ctgctcgagg
     1741 agcctggtca tcgctgtgat gccatcgatc gtcactccgg acacgttcgc ccaggcgggt
     1801 tgggcggggg tccgtccgtg gtacgaggaa cttgaggcgc gcccactcgg cgcgggatcc
     1861 ctcgaagcct ggctggcgga ctggtcccgc ctcgagtcgg ccctgggcga ggccgcggcg
     1921 cgtgcgatga tcgcgtacac gtgtgacact gacaatgcag cgaagctcga tgcccatctg
     1981 cgcttttcca gtgacatcct ccccaaggcc gaggagctga gcgtccgcct tgcccgccgg
     2041 ttcgttgcct tcggcgtggt gccgccgggc ctcgagcagc cggtggcccg gtttcggcgg
     2101 gccatcgagg tcttccgcga ggccaacgtc ccgttgttca ccgagaccga gcgcctcggg
     2161 tcggaatacc agcagatcac cgggggcctc acggccgcgt ggggcgacga gacccttccg
     2221 ctgccgcggc tcgcgccctt cctcaagagc atcgaccgcg cggagcgcga gcgggccttc
     2281 cgggcgtggg ccgcacccta tatcggacgg cgcgctgagt tcgcggcgtt gttcgaccgg
     2341 atgtacgtcg cccggcagga ggtcgcccgg aatgcggggt tcgccgactt ccaggcctac
     2401 agcttcgcgg cgaagtgccg gttcgattac acgccggatg actgccgtcg gttccacgat
     2461 tcggtcgagc aggcggtgat gccggcggtc gagcggatcc acgcccggcg ccgagcccgg
     2521 ctcaagctgc gggcgctccg tccctgggac acgaacgtgg atctgtgggg acgcccggcc
     2581 atccggccgt tctcctccga ggcggaattc atcgcgaccg ccgagcgggt gttccaacgg
     2641 gtcgacccgg cgctgggccg ggagttcgcc acgatgcgcg aggagcggct ccttgacctg
     2701 acgagccgca ccgggaaggc gccgggcggc tactgcgaga ccctgagtgc gagcggccgg
     2761 ccgttcatct tcatgaacgc cgccgggacg gtcgaggacg tgatgacgct gctgcacgag
     2821 tcgggacacg cgttccacgc gttcgcgtcc catccgctcc cctatctctg gcagcgccac
     2881 ccgacgatgg aaatggcgga gctcgcgtcg atgtcgatgg agctgctggc gttctccgcg
     2941 ctccgaaagg ccgacggcgg gttcttctcc gacgccgagc accgccgcgc caagcgcgaa
     3001 cacctggagg atgcgctcct cacgctcgcg cacgtcgcgt cggtggacgc cttccagtcg
     3061 tggatctaca ccagcggcgc cggcggggac gctgcggcgc gcgaccaggc ctggctccgg
     3121 atccgcgggc ggttcgagcg gggcatcgac tggtcggggc tcgatgcgga gcgggtggcc
     3181 cggtggtacc gccagctgca catcttcctc tatccgttct attacatcga gtacgggctg
     3241 gccggtcttg gggcgctgca gctgtggcgg aacagcctgg ccgatcatcc cggtgcactc
     3301 gccgcgtacc gccgggccct tgcgctcggc ggcacccggt ccttgccgga gctgtaccgg
     3361 gccgccggcg cacggctcgt gttcgacgcg gcggggatgg cggagctggt ggcggtggtg
     3421 gaggaggaac tgggccgcct cgacgagggg gagccgggcc gttgattccc gggcccgggg
     3481 aggcgatgtt tccgccttac ccttggggag cggagtggat ggccaaagaa gaaggcatcg
     3541 aaatggaagg cgtcgtgacc gaggtgctgc ccgatcggaa ctatcgggtg cttctcgaga
     3601 acggccactc cgtgctggcc tacgcggccg ggaagatgag caagttcaag atccgggtac
     3661 tcgagggcga ccgcgtgagc gtggtgctct cgccgtacga cctgacccgc ggccgggtca
     3721 tctaccgcca taagtagcag gaggcgcctg cggaggcccg cagttccagg cccgccccct
     3781 cccccgaaac acgacgggcc gcctcttgcg aggcggcccg tcacgtttct ccgctgggtc
     3841 ttacttgacc ttggtcacgt tctcagcggc gggccccttg gcgcccatca ccatgtcgaa
     3901 ctcgaccttc tcaccctcgg ccaggctgcg gaagccgtcg gccttgatcg cggtgtggtg
     3961 cacgaagcag tccttctcgc cgttctccgg ggtgatgaag ccgaacccct tggcgtcgtt
     4021 gaaccacttc acggtgccct gcatgcgtgc catcgaacta acctcgtgtt atgtgactcg
     4081 tcggggtccg gttcaacggc accatccgac tccttgtgga ccgcgggcct gtcccgcgtc
     4141 ccaagctctg aaaagaccag cgcgaagcgc cggtcttcga ttccagctgc tgcacttcaa
     4201 gatgccgggg gatgaaaaag gaaccgccgg acaacgttca aagctcgccc ggcggcccca
     4261 ttcctgtcaa gggccgggcg cctaacccct ttcaggggcg cgacttccac caccgaagcg
     4321 cctgtttggt ccactcgccg ctccggtgcc ggaggccggt cacccgattg agggcctgct
     4381 ggaactcggc tgccgatgga tagcggagct ccgcttccga ctgaagcgcc cgttcgagga
     4441 cctcgaccag ctgcaggcgc acgtcggggc gccgccgctg gagctcgggg aagcgattgg
     4501 cggtctggcg cgcgaggacc tgctccggcg tgctgccggg gaacggcggc tcgccgagga
     4561 gcgcgaagta cgcgaccgcg ccgaggctgt acaggtcggt ccgctggtcc acccgctcgc
     4621 cgagcagctg ctcggggctc gcgaactgcg gcgtcccgct ctggctcgtg gcgccgccga
     4681 acttcccctg gccccggagc gccagcgcga gcccgaagtc ggtgatccgg agccgaccgt
     4741 cgcgctcgag gaggaggttc tccggcttca cgtcccggtg gacgagcccc tgctggtggg
     4801 cgtggcccag cgcggagagc gcctcccgga ggagccgcat gaggtcgtcg accgggaggg
     4861 ggccgtcgcg ttccaccttc tgggcgacgt tgggcccgtc gaccagctcc atcgtgtacc
     4921 agagcaggcc ggagcggccg gcgatgtcgt agatgtcgac gatgttcgga tggttgaggc
     4981 gggcggcgag ctgggcttcg cggcggaacc gttcgaccac ggcgggatcg gcggtcaggt
     5041 gcgggtgcat cactttgagc gccacctcgc gttcgaggtg caggtcccgc acccggtaga
     5101 cccggccgaa tccgcccgcg cccaggagcg aaaggagttc gtagtcgtcg cccagggcgc
     5161 ggcggagccg tttctcgtag agcccggtgt cttcgaagcc ggtcgccccc gtccgggcgg
     5221 ccggtaccat cagcgcggga ttgtcgaacg tgccggcgtc ggaggcgaag tcctcgagca
     5281 tctccgacgc cgtcaggtag cggtcggcgg gattgggccg gagggcccgg agcaggatcc
     5341 gctcgatggc ccgcggcgtg gcgggccgga gctccatcgg ccgcttgagg gtggtgcagt
     5401 cgaggggcgg ctcgacaccg gtgatggcgt agtagaggat cgcgccgacg gtgtagacgt
     5461 cgctggcctg gtcgcccacc tggccgtcgc ggacctcggg cgccatgaac ggcagccccg
     5521 aggggcgttc atccggcggg atcatcggca gcgtccagct ggagaaccgg agttcggtca
     5581 ccaccccgcg gccggccagg ttgaggagca ccgcagcagg cacgatgcgg cgggcgatga
     5641 tcccgtgcgc gtgcgcgtgt tccagcgccg acaggaggtc gcgggcgagg gagtggaccg
     5701 cggggatggg gcgggggccc cgctgcaccg cttcctggag gctctccccc tcgatccagt
     5761 tgcccacccg gaaggccacg tccccggcga cgccggcatc gtaggcgtgc cggatcgcgg
     5821 ggtggtcgag ttgtcccagc gcttcggcct ctcgcaggaa ccagcggcgg atggcctcgt
     5881 cggtgtagaa gttgacccgg agcgacaccg gccgattgag caccgtgtca taggccgcgt
     5941 agagcacccg ctcccgggac gatgcgacga gcgtatcgag gcggtagcgg gctcccacgg
     6001 ccgccttgac acggtcgtgc gtggccagcg ggggcggggt ggacgtcacg ggggtggtgg
     6061 ccacgggact cgcggttcgg gggtcgccag caaccgacat aatatagcca actagctgcc
     6121 gtgcttggag ttacggttct gtcacaagcc gggggttgcc agcggcaggc tgatatggta
     6181 gcattatccc cgttgcaggt gatgtgacat agcccatttg ggccccaggg gtttggcttg
     6241 cagcctgggt gaacaatcct gacaagccca actccccggc gcgttctacg cgctggggtt
     6301 tttgcttgct aggccccgag ttcgctggcg aggtcccgcg tcagcagtcg cagctctccc
     6361 agcatttccc gccgcggcca cgcgtcgtgg cgatcgagcg tttccgccac ggagcggaac
     6421 caccagacga gctgggggcc ggagaccgcg cggctgttgg ggaggtactc ccggccgagc
     6481 cggcgcacgt cgctcaggat gctgccgcaa tcgaggatct cctgcgcggc gcggagcgag
     6541 agcgtgcggg gttcgagcat cgcgaggcgc gcgagccggt ccacgcactc ggtctcccac
     6601 gtggtccgca ggccccgcga ggccggcggg tcctggggct ggtcttcgag cgccaccgcg
     6661 agcgcgaccg gcccgaactt ctcgccgagg cgtcggatga ggtcggcgcg ctcctgcgcg
     6721 cgcgactggg cgatcacctc atggagcacg gcggcgacga cggtgacctc gtcgcatccg
     6781 tacgacgcga ggatgacggc caggttcgcg ggccgtgcca gggcggcggc ggggcgggtg
     6841 gcgtcgcgcg agaggtgctt cgccgcgaag gccagcgcgt gatgggtgcg gtcggaatag
     6901 ccggtcacgg gatgccgagg agcttgtggg tctggaggga gaggcgccac cggggatggt
     6961 cgaggcagta gcgcaaggcg agcgccgtgt tccggtcgcg gtcggggccg tccatcggct
     7021 gcaggaagaa gtggtcgaag tcgagcgatc cgtagcgttc gggcccgccg ccgggctggg
     7081 gaaagaccag cttgagttca tcgccccggg tgaggacgag cggcgcgccg gacttggggc
     7141 tcacgcagat ccagtcgatc cccggcggcg cgggctgggt gccgttggtc tcgacggcga
     7201 cgcggaatcc ggcggcgtgg aacgcgtcga tgagcgcgcg gtcgagctgg aggagcggct
     7261 cgccgccggt gcacaccacg agcgccgacc cgggcgacgt gcgggcggcg cgccgcgccg
     7321 gatcgccgtc atcccagatc tccgcggcac ggcgcgcaag cgcctccgcc gaggcgaact
     7381 tgccgccgcc ggggccgtcg gtccccacga actgcgtgtc gcagaaggtg cagaccgcgc
     7441 cagcccgatc ctcttcccga ccgctccaga ggttgcagcc ggcgaaccgg cagaagaccg
     7501 ccggcgtgcc ggcctgggcg ccttcgccct gcagggtgta gaagagttcc ttgacggcgt
     7561 agggcatgtc gaaaagtaag cggtgagggg ggagcggtga gcggtaaacg gtaaacggtg
     7621 aacggtgaac agtgaacggg cctcccttca acgacccgtt gaccattcaa cgttcaccgt
     7681 tcacgctcga catacaccgc cggatcctcg atcccgttct ccgcgaatcc ccggagccgg
     7741 agccggcagg cgtcgcagcg cccgcaggcg cggccgtcgg gcgtcgggtc gtagcaggtg
     7801 agggtcgcgg cgtagtcgac gccgagcgcg cgcccgcggg cgatgatctc gcgtttggtg
     7861 agcgtg
//
LOCUS       DASUAP010000064         6163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_369329, whole genome shotgun sequence.
ACCESSION   DASUAP010000064 DASUAP010000000
VERSION     DASUAP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369329"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2824
                     /locus_tag="VFU45_01570"
     CDS             <1..2824
                     /locus_tag="VFU45_01570"
                     /inference="COORDINATES: protein motif:HMM:NF039643.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEU5357778.1"
                     /translation="WRRRDRHPELKRVEVIDATGRRVTDAWVPRLDRESGDVVFAAAS
                     AGDYDIYYMPYQGTFRSNYPKITYRKPDTTASGEWRVAGGVDSAGLLAEKWRALPQAT
                     VAEFDDVDSLASVYPMEVIATAAETDALRRAHPDAAYLLFPEDRTRPIKMRHSLPERW
                     TSGAGGAVTGTADRGEFYAFQVGVWALRPVGGVTVRFSGLTGPGGGRIPASAFQSFNT
                     GGVDWQGRPFARTLSVDSGAVQALWCGVMVPDSAAAGAYDGTVTVTAAGVATTLPIHL
                     TVTSALIPDHGDDEPWRLARLRWLNSTLDLDTTLVPPYTPVSVKGTTLSILGRTVALA
                     PSGFPRQVTSWFTPEMTAIGRKPRGLLAEPVALTVVDSGGREVRWAHGPVRFVRRMPG
                     AAVWEVRSSAGAVSATTHAQMEFDGNIDYTVALTASHPTPLADVRLDVPYRRSVAKYF
                     MGMGEKGGLTPDSYDWAWDVKKNQDAVWIGDVNAGMQVTLSDQHYIRPLNTNFYQLRP
                     LVMPESWDNGGKGRCHFGGDGPVAYRMRCTSGARMLAPGDTLYFNVRLLVTPFHPIDP
                     KAQFSTRFYHAYVPVTQVADSGANVVNVHHATDINPFINYPFLRPREMRAYADSAHAI
                     GIRFKIYYTVRELTNHAPEIWALRSLGNEVLADGPGGGHSWLQEHLVDHYITGWVVPE
                     RRDVAVVTSGISRWHNFYVEGLDWLVKHEQIDGIYLDDVAFDRTTMQRIRRVLARGRP
                     HPMIDLHSANQYDKNDGYASSANLYLEHFPYIDRLWFGEYFDYNSQPDYWLTEISGIP
                     FGLMGEMLQDGGNPWRGMVFGMTDRLPWAGDPRPLWLAWDAFGITDARMLGWWAPSDP
                     VKTGRSDILATSYVRKGRTMVAVASWAKDTVALHLSIDWKALGLDPRTATISAPAIEK
                     FQPAAMFKVGDPIPMVPGKGWLLVIK"
     gene            2877..4502
                     /locus_tag="VFU45_01575"
     CDS             2877..4502
                     /locus_tag="VFU45_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU5357779.1"
                     /translation="MAAASVLARPHGVAGSAVRSASRKPALPSPQQVAWQDLQLGMFV
                     HLAPNTWTDKEYDDLSLSPEDVVTNIDAEQWADVALSLGAKYVVLVAKHAGGYCLWQT
                     DTTDYSIRNSPWKDGRGDAMADLSRACKERGLGLGVYLSPRDDKHGAGVSGRCKTPTA
                     QAAYNALYREQLTEVLGRYGPIVELWLDGNSVVPTGDIVARLQPGAMVFQGPQATIRW
                     VGNEDGFAPYPCWNALTVEDALDGSATALEGDPDGEVWRPVEADVSVRRPDWFWSTTN
                     EKDLLSLDELVEIYYRSVGHGTQLLVNFPPDRSGRIPAVDAARAKEFGDLIRHRFGAP
                     VAEVSGRGKKLTVEFGVERRVDHVVLQEDIAQGERVRSYEIEARIDDKWRVIGAGTAI
                     GHKHIQPIGGVVADALRLHVHHSDGKPQIRRFAVHNTGAEPPKTWNLQPELTGDDVVG
                     GWTGDRFEVDLTPHLDRAALYRIRFAGIHGEPVKVQWIELLEGGDSAPQLIRREKKRK
                     DAFLVSVPEMGRAVVVRGRILGADAGWVLLRKG"
     gene            4666..5781
                     /locus_tag="VFU45_01580"
     CDS             4666..5781
                     /locus_tag="VFU45_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU5357780.1"
                     /translation="MGTKLALERAPRTAKPAAAGTMKAFVMHGIGRVGFMDKPVPADP
                     GPDDAIIRTTHALICTSDTHTVAGAIGARSDLTLGHEAVGIVARIGANVKGVQLGDRV
                     AVNAITPCYKCDNCQRGFTSQCTEMLGGWKFGNTKDGVFAEYFHVNDADANLAVIPAT
                     VPDDKAVYATDMMSTGFMAAEHANIPIGGSVAIFAQGPVGLMATVGARLLGAGLVIAV
                     ESAPKRKDLATRFGADVVVDHTKMDAVEAIRSLTNGHGVDSAIEALGSEETFDACIRA
                     TRPGGTISNVGYHGKGEYLRIPRVAWGVGMSDKTIRTGLCPGGRERMMRLLRLIENGR
                     VDPTPLTTHRFPFAELDRAFELMSTKGDRIIKPLITF"
     gene            complement(5833..>6163)
                     /locus_tag="VFU45_01585"
     CDS             complement(5833..>6163)
                     /locus_tag="VFU45_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357781.1"
                     /translation="LGLGTDSTTVVYGPGSRDLDEAPRFLSCRWAGLPSTPGDMSASL
                     RYVVRPDGSVDSATVGVSVASSRSGAPKAEAFARALVTHCSFAPARVLGRPVPAVVEH
                     SITVRHN"
BASE COUNT          984 a         2143 c         2142 g          894 t
ORIGIN      
        1 gtggcggcgc cgggaccggc atccggagct caagcgggtc gaggtgatcg atgcgaccgg
       61 gcgccgggtg accgacgcct gggtcccgcg gctggaccgg gagtcggggg acgtcgtctt
      121 cgccgcggcc tcggcaggcg actacgacat ctactatatg ccgtaccagg ggacgttccg
      181 gtccaactat ccgaagatca cgtatcggaa gccggacacg acggcgagtg gtgagtggcg
      241 agtggcgggc ggcgtggact ctgccgggct gctggccgag aagtggcgcg cgttgccgca
      301 ggccaccgtc gccgagttcg acgacgtcga ctcgctcgcg tcggtctatc cgatggaggt
      361 catcgccact gcggcggaga ccgacgcgct ccggcgcgcg caccccgatg ccgcctacct
      421 cctctttccg gaggaccgca cccggccgat caagatgcgg cactcgctcc cggaacggtg
      481 gacatcgggc gcggggggtg cggtcaccgg caccgccgac cgcggggagt tctacgcttt
      541 ccaggtgggg gtctgggcgc tcaggccggt cggcggcgtc accgtccgat tcagtggcct
      601 gacggggccc ggcggcgggc ggatcccggc gtcggcgttc cagtcgttca acaccggcgg
      661 cgtcgattgg cagggccgcc ccttcgcgcg cacgctttcg gtcgacagcg gcgcggtgca
      721 ggcgctctgg tgcggggtga tggtgcccga cagcgccgcg gccggtgcct acgacggcac
      781 ggtgacggtc accgccgccg gggtcgcgac gacgctgccg atccacctca cggtcacctc
      841 cgcgctcatc cccgaccacg gcgacgacga accctggcgc ctggcccggc tccgctggct
      901 caacagcacc ctcgacctcg ataccacgct ggtcccgccc tacacgccgg tctcggtgaa
      961 gggcaccacg ctctcgatcc tcggccgtac ggtggcgctc gctccgagcg gcttcccccg
     1021 gcaggtcacc agctggttca cgccggagat gaccgccatc gggcggaagc cgagggggct
     1081 cctggcggag ccggtggcgc tcacggtggt ggatagcgga ggtcgggagg tccggtgggc
     1141 gcacggcccg gtgcgcttcg tgcggcggat gcccggagcg gcggtgtggg aggtccggag
     1201 cagcgcgggt gccgtgtccg ccacgaccca cgcgcagatg gagttcgacg gcaacatcga
     1261 ctacaccgtg gcgctcaccg cgtcccatcc gacgccgctc gctgacgtcc gcctcgacgt
     1321 gccgtatcgc aggagcgtgg cgaagtactt catgggcatg ggcgagaagg gcggcctcac
     1381 gcccgattcc tacgactggg cctgggacgt gaagaagaac caggacgccg tctggatcgg
     1441 cgacgtgaac gcggggatgc aggtgacgct ctccgaccag cattacatcc gtccgctcaa
     1501 caccaacttc taccagctgc gcccgctggt gatgcccgag agctgggaca acgggggcaa
     1561 gggcaggtgc cacttcgggg gtgacggccc ggtggcctac cggatgcggt gcaccagcgg
     1621 cgcgcggatg ctggcaccgg gcgacaccct ctacttcaac gtccgcctgc tggtgacccc
     1681 gttccacccg atcgacccga aggcacagtt ctccacccgg ttctaccatg cgtacgtgcc
     1741 ggtgacccag gtggccgatt ccggcgccaa cgtggtgaac gtccaccacg ccaccgacat
     1801 caacccgttc attaactacc cgttcctgcg tccgcgggag atgcgcgcct acgccgacag
     1861 cgcccacgcg atcgggatcc ggttcaagat ctactatacc gtccgcgagc tcaccaacca
     1921 cgcccccgag atctgggcgc tccggagcct gggcaacgag gtcctggccg acgggcccgg
     1981 cggcggccac tcctggctgc aggagcacct cgtcgatcat tacatcaccg gctgggtggt
     2041 gcccgagcgg cgggacgtgg ccgtggtgac gagcggcatc agccggtggc ataacttcta
     2101 cgtcgagggg ctcgactggc tggtcaagca cgagcagatc gacgggatct acctcgacga
     2161 cgtggcgttc gaccgcacca cgatgcagcg gatccgccgg gtgctggcgc gcgggcggcc
     2221 gcacccgatg atcgacctgc acagcgccaa ccagtacgac aagaacgacg gctacgcctc
     2281 gagcgccaac ctctacctcg agcacttccc ctacatcgac cggctctggt tcggggaata
     2341 cttcgactac aactcccagc ccgactactg gctgaccgag atctccggga tccccttcgg
     2401 cctgatgggg gagatgctgc aggacggcgg caacccctgg cggggcatgg tcttcgggat
     2461 gacggaccgg ctcccctggg ccggtgatcc gcggcccctc tggctggcgt gggatgcctt
     2521 cgggatcacc gacgccagga tgctggggtg gtgggcgccg tccgacccgg tgaagaccgg
     2581 ccgctccgac atcctggcca ccagctacgt gcggaagggc cggacgatgg tggccgtggc
     2641 cagctgggcg aaggacaccg tggcgttgca tctttccatc gactggaagg cgctgggcct
     2701 cgaccctcgg accgccacga tctcggcgcc ggccatcgag aagttccagc cggcggccat
     2761 gttcaaggtg ggcgacccga taccgatggt gcccggaaag gggtggctcc ttgtcatcaa
     2821 gtgatcggcg ttccgggccg gggggtgagg tccctcgccg caccttcctc gccacgatgg
     2881 ccgcggcgtc ggtcttggcc cgtccgcacg gtgtcgcggg ctccgccgtc cggtccgcga
     2941 gccggaagcc ggccctgccc tcgccgcaac aggtggcgtg gcaggacctc cagctcggga
     3001 tgttcgttca cctggccccc aacacctgga ccgacaagga gtacgacgac ctctcgcttt
     3061 cccccgagga cgtcgtcacc aacatcgacg ccgaacagtg ggccgacgtc gcgctgtcgc
     3121 tgggggcaaa gtacgtggtg ctggtggcca agcacgccgg cgggtactgc ctctggcaga
     3181 ccgacaccac cgactactcc atcaggaatt caccttggaa ggacggccga ggcgacgcga
     3241 tggccgacct ctcccgcgcg tgcaaggagc gcggactcgg gctcggcgtg tacctgagcc
     3301 cgcgcgacga caagcacggc gccggcgtga gcggccgctg caagaccccc acggcccagg
     3361 ccgcctacaa cgcgctctac cgcgagcagc tcaccgaggt gctcggccgt tacggaccca
     3421 tcgtggaact ctggctcgac ggcaacagcg tcgtccccac cggtgacatc gtcgccaggc
     3481 tgcagccggg ggcgatggtg ttccagggcc cccaggccac catccgctgg gtggggaacg
     3541 aggacggctt cgcaccgtat ccctgctgga acgccctgac cgtggaggat gccctcgacg
     3601 ggagcgccac cgcgctcgag ggagatcccg acggcgaggt gtggcgtccc gtggaggccg
     3661 atgtctcagt gcgccgcccc gactggttct ggagcaccac caacgagaag gatctgctct
     3721 cgctcgacga actggtcgag atctactacc ggtccgtggg tcacgggacg cagctcctcg
     3781 tcaacttccc cccggaccgg agcggccgga tcccggcggt ggacgccgcg cgtgcgaagg
     3841 agttcggtga cctgatccgg caccgcttcg gcgcgccggt ggccgaggtg tcggggcggg
     3901 gaaagaagct gaccgtcgag ttcggggtgg agcggcgggt ggatcacgtg gtgctgcagg
     3961 aggacattgc gcagggagag cgggtgcgga gctacgagat cgaggcgcgc atcgacgaca
     4021 agtggcgcgt catcggcgcc ggcaccgcca tcggacacaa gcacatccag cccatcggtg
     4081 gcgtcgtggc cgacgcactc cggctccacg tccatcattc cgacggaaag ccccagatcc
     4141 gccgattcgc ggtccacaac accggcgccg agccgccgaa gacgtggaac ctccagccgg
     4201 aactgaccgg cgacgacgtc gtcggcgggt ggaccggcga ccgcttcgag gtcgacctca
     4261 cgccgcacct agaccgggcc gccctctacc ggatccgctt tgccgggatc catggcgagc
     4321 cggtcaaggt gcagtggatc gagctgctcg aaggcggcga ctcggcaccg cagctcatcc
     4381 gccgggaaaa gaagcggaag gatgccttcc tcgtgtcggt gcccgagatg ggccgtgcgg
     4441 tggtggtgcg gggccggatc ctgggtgcgg atgccgggtg ggtgctgttg cggaaggggt
     4501 gatcccacgg acgatcgttc catgccctga accccttctc atcgcccctt cacggcccta
     4561 tcaccgcctc ggcgccacct tcctgacccc ccaacggaag tgtggcgccg ctcgcatccg
     4621 atatcccatc gggacgcgcg cgccgcaagg cagcggaggt tgcagatggg aaccaaactc
     4681 gccctcgaac gcgcgccgcg caccgccaag cctgcggccg ccgggaccat gaaggcgttc
     4741 gtgatgcacg gcatcggccg tgtggggttc atggacaagc cggtgccggc cgacccgggc
     4801 cccgatgacg ccatcatccg gaccacccac gcgctcatct gcacgtccga cacccacacg
     4861 gtggcgggcg ccatcggggc gcggagcgac ctgacgctgg gccacgaggc ggtggggatt
     4921 gtcgcacgga tcggcgccaa cgtgaagggc gtccagctcg gcgaccgggt ggcggtcaac
     4981 gccatcaccc cctgctacaa gtgcgacaac tgccagcgcg gcttcacgtc gcagtgcacc
     5041 gagatgctgg gcgggtggaa gttcggtaac acgaaggacg gcgtcttcgc cgagtacttc
     5101 cacgtcaacg acgcggacgc caacctggcc gtcatcccgg ccaccgtccc cgacgacaag
     5161 gccgtctacg ccaccgacat gatgtcgacc gggttcatgg cggcggagca cgccaacatc
     5221 cccatcgggg gatcggtggc catctttgcc caggggcccg tcggcctgat ggccacggtc
     5281 ggcgcccgcc tcctcggcgc cgggctcgtg atcgccgtgg agagcgcgcc caagcggaaa
     5341 gacctcgcca cccgcttcgg cgccgatgtg gtggtggatc acaccaagat ggacgcggtg
     5401 gaggccatcc gctccctcac caacgggcac ggagtcgact ccgccatcga ggcgctcggt
     5461 tcggaggaga ccttcgacgc ctgcatccgc gccacccgcc ccggcggcac catctccaac
     5521 gtgggctacc acgggaaggg cgagtacctc cggatcccgc gcgtggcgtg gggggtgggg
     5581 atgagcgaca agaccatccg caccgggctc tgccccggcg gccgtgaacg gatgatgcgg
     5641 ctcctgcggc tcatcgagaa cggccgggtg gacccgacgc cgctcaccac ccaccggttc
     5701 ccgttcgcgg agctggaccg ggcgttcgag ctgatgtcca ccaagggtga ccggatcatc
     5761 aagccgctga tcaccttctg atcggcacgg cggcatgcgc ggagggggcg gcagccccct
     5821 ccgcgcggcg tttcagttat gcctgaccgt gatcgaatgc tccaccacgg ccggcaccgg
     5881 gcgcccgagc acccgggcgg gcgcgaaact gcagtgcgtc accagcgccc gggcgaatgc
     5941 ctcggctttc ggagcgccgg acctcgaact ggcaacggac acaccgacgg tcgcggaatc
     6001 cactgagccg tccggtcgga cgacgtaccg caagctggcc gacatgtcgc cgggtgtgct
     6061 cggcagaccg gcccagcggc aggacaggaa ccggggcgct tcgtcgaggt cccggctgcc
     6121 agggccatac acgacggtgg tggagtcggt gccgaggccg agt
//
LOCUS       DASUAP010000065         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_859978, whole genome shotgun sequence.
ACCESSION   DASUAP010000065 DASUAP010000000
VERSION     DASUAP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859978"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2096)
                     /locus_tag="VFU45_01590"
     CDS             complement(<1..2096)
                     /locus_tag="VFU45_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEU5357782.1"
                     /translation="MQLNVFETANAGFAQAMYEEYLRDPASVAPEWRRLFDSGVVGER
                     PAAVIGSGNGSAPAPTSAGAAPSAPVPAGAVEIKGPAARLVQNMNESLRIPTATTFRE
                     INVTTLDARRRELNAALAASGKPGKVSFTHLVAWSLVQGLKRHPMMGHALQEQGGTAY
                     RVIPDGINLGLAVDVQRKDGSRGLVVPVLKHAEAMDFGGFHATYEELVEKARTNKLMP
                     DAFQGATVTLTNPGGLGTSASVPRLMAHQGTIIATGAIGYPAAFAATPPDQLKALRVG
                     KVMAMTSTYDHRIIQGAESGEFLRTVESLLQGDAGFFEGVAAGLGVAIGAAPAAAAPL
                     PAVKAAPLSEAPISPDALAQVAAAMGLVKAFRTHGHLAARLDPLGSDPIGDPALEPGP
                     LGLTPEIMRTIPAPTLRVAVPGATLADALPRLRETYCGTIAYEVEHISSHEERVWLRE
                     AIESGRYRRPLTPAERTALLARLTQVETFEKFLHKAYLGQRRFSIEGVDLLVPMLDLT
                     TDLAAQQGARDVVIGMAHRGRLNVLVHNVGRPYEAIFAEFEGNKPQHDEPDAGTGDVK
                     YHLGADGAVRAANGKTVSITLSPNPSHLEFTGPVVDGRARAQQTQRKGPTAEHDPTAA
                     VPVVIHGDAAFAGQGVVAESLNLSKLPGYGTGGTVHIITNNQIGFTTDIKDARSTRYA
                     SDLAKGFDIPIVHVNA"
     gene            2367..>3279
                     /locus_tag="VFU45_01595"
     CDS             2367..>3279
                     /locus_tag="VFU45_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5357783.1"
                     /translation="MRSSSAIGSVILAAALAACGGGGGGSPSPSLSLSKPAASGDAQQ
                     AEVGHAVASAIKVLVKLDTAVSAGHTVTWSASAGGSASPPTSQSGPDGVASTTWTLGS
                     TPGGQTLTATLAGAAGSPVTYTATAIAPVVAKATVSGDAQSDTVHATLATPLGVEVTL
                     SGVPLSGRTIQWATANPGGVVTAIDTTASTTGIATAHWKLGTVAGPQSATATTVGAVA
                     GVTFTATAKPGLAKTLSKASGDSQVVDTSHALANPLVVKAVDQFGNARSGVTVQWNVT
                     SANATVSPSSDTTAVNGTASATVTSGSS"
BASE COUNT          426 a         1187 c         1177 g          489 t
ORIGIN      
        1 tccgcgttga cgtggacgat cgggatgtcg aaccccttgg cgaggtcgga cgcgtagcgg
       61 gtggagcgcg cgtccttgat gtcggtggtg aagccgatct ggttgttggt gatgatgtgg
      121 acggtcccgc ccgtcccgta cccggggagc ttgctcaggt tgagcgattc cgccaccacg
      181 ccctggcccg cgaacgccgc gtcgccgtgg atcacgacgg gcacggcggc ggtcgggtcg
      241 tgctcggcgg tcgggccctt gcgctgggtc tgctgcgccc gcgcccgacc gtcgaccacc
      301 ggcccggtga actcgaggtg gctcgggttg ggggagaggg tgatgctcac cgtcttgccg
      361 ttggcggccc gcaccgctcc gtcggcgccg aggtggtact tcacgtcgcc ggtgccggca
      421 tcgggctcgt cgtgctgggg cttgttcccc tcgaactcgg cgaagatcgc ctcgtacggg
      481 cggccgacgt tgtgcaccag cacgttgagc cggccgcggt gcgccatgcc gatcaccacg
      541 tcgcgcgcgc cctgctgggc cgcgaggtcg gtggtgaggt cgagcatcgg gaccagcagg
      601 tcgacgccct cgatcgagaa gcggcgctgg ccgaggtacg ccttgtggag gaacttctcg
      661 aacgtctcca cctgggtgag ccgcgccagc agcgcggtgc gctccgccgg cgtgagcggc
      721 cggcggtacc gccccgactc gatcgcctcg cgcagccaca cccgctcttc gtggctggag
      781 atgtgttcca cctcgtaggc gatggtgccg cagtacgtct cccggaggcg cggcagcgca
      841 tcggccagcg tggcgcccgg gaccgccacc cgcagcgtcg gcgccgggat ggtgcgcatg
      901 atctccggcg tgaggccgag cggcccgggc tcgagcgccg ggtcgccgat gggatcgctg
      961 ccgagggggt cgagccgcgc cgccaggtgc ccgtgggtgc ggaacgcttt cacgagcccc
     1021 atcgccgccg ccacctgcgc cagcgcgtcg ggcgagatgg gcgcctcgct gagcggggcc
     1081 gccttgaccg cggggagcgg cgccgccgcg gccggtgccg cgccgatcgc gacgccgagc
     1141 cccgctgcca ccccttcgaa gaagccggcg tcgccctgca gcagcgactc caccgtccgg
     1201 aggaactcac ccgactccgc gccctggatg atgcggtggt cgtaggtgct ggtcatggcc
     1261 atcaccttcc cgacccgcag cgccttcaac tggtcgggcg gcgtggcggc gaaggcggcc
     1321 gggtagccga tcgcgccggt cgcgatgatc gtgccctgat gggccatcag ccgcggcacc
     1381 gacgccgacg tgccgaggcc cccgggattg gtgagcgtca ccgtcgcgcc ctggaaggcg
     1441 tccggcatca gcttgttggt gcgcgccttc tcgaccagct cctcgtacgt ggcgtggaat
     1501 ccgccgaagt ccatcgcctc ggcgtgcttg agcaccggga ccaccagccc gcgcgacccg
     1561 tccttgcgct gcacgtccac cgcgaggccc aggttgatcc cgtcggggat gacgcgatac
     1621 gccgtgccgc cctgttcctg cagggcgtgc cccatcatcg ggtggcgctt caggccctgc
     1681 accagcgacc acgccaccag gtgggtaaag gagaccttcc ccggcttccc cgacgcggcg
     1741 agcgcggcgt tcagctcccg ccggcgcgcg tcgagggtcg tcacgttgat ctcgcggaag
     1801 gtggtggccg tcgggatccg gaggctctcg ttcatgttct ggacgagccg tgcggccgga
     1861 cccttgatct ccaccgcgcc ggcggggacc ggggccgatg gtgccgctcc cgcggacgtg
     1921 ggcgcgggcg cggatccgtt gccggagccg atcaccgctg ccggccgctc gccgaccacg
     1981 ccgctgtcga agaggcgccg ccactccggc gccaccgacg ccggatcccg gaggtattcc
     2041 tcatacatcg cctgcgcgaa tccggcgttg gccgtttcga acacgttcag ttgcacggac
     2101 ttccgctcca ccaggaccgt gggatcgtgg ggacccattc cccgccggcc gcagccggtt
     2161 cgagcgccaa aggtaacgcg ccgcccggcg ccttgcaggg cggagcccca ccggcgggaa
     2221 cctgggtacg gccgtccgtc gtattccgta tgggtgtgag tgttccgggc tgcaccgtca
     2281 ttccagagtc catcgttgat cggtccgttc cccgtcacgg cgctcccggc gtcgtgcgcg
     2341 atatttcttc tcgtcggagg tcaaggatgc ggtcatccag tgccatcggg tcggtcatcc
     2401 tcgcggctgc gctcgcggcg tgcggcggcg gtggcggagg gagcccctcg ccgtcgctca
     2461 gcctctccaa gcccgcggcg agcggcgatg cacagcaggc ggaggtgggg cacgccgtcg
     2521 catcagccat caaggtcctg gtgaagctcg acacggcggt gtcggccggg cacaccgtca
     2581 cctggtccgc gtcggccggc gggtcggcga gccccccgac cagccagtcg gggcccgatg
     2641 gcgtggcctc gaccacctgg acgctcggtt ccacgccggg cggacagacg ctgacggcca
     2701 cgctcgcggg cgcggccggt tcgccggtga cgtataccgc caccgcgatc gcgccggtgg
     2761 tggcaaaggc gacggtgagc ggcgacgccc agtccgacac cgtgcacgcc acgctcgcga
     2821 cgcccctggg cgtcgaggtg acgctcagcg gcgtgccgct ctccgggcgg acgatccagt
     2881 gggccaccgc gaaccctggc ggggtcgtga ccgccatcga caccaccgcc agcaccaccg
     2941 gcatcgcgac ggcacactgg aagctcggca cggtggcggg cccccagtcg gccacggcca
     3001 cgacggtggg cgccgtcgcg ggcgtcacct tcaccgccac cgccaagccc gggctcgcca
     3061 agacgctcag caaggcctca ggcgacagcc aggtcgtcga cacgtcacac gccctggcca
     3121 atccgctcgt cgtgaaggcg gtcgaccaat tcggcaacgc gcggtccggg gtcaccgtgc
     3181 agtggaacgt cacgtccgcg aacgccaccg tgtcgccgtc gagcgacacg acggccgtga
     3241 acggcaccgc gtccgcgacg gtcacgtcgg gatcttcca
//
LOCUS       DASUAP010000066         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_601312, whole genome shotgun sequence.
ACCESSION   DASUAP010000066 DASUAP010000000
VERSION     DASUAP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601312"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFU45_01600"
     CDS             <1..459
                     /locus_tag="VFU45_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357784.1"
                     /translation="YTTVLRGWKAFGDYATWWIDGRVLASTTSGTVPLDAQFFIPTWE
                     RSIRSYGGAETNRGLVDQRYTGPDLLAATLDVNRDLVNVGLYGAVSVHAFVDAGRVFD
                     TGGFQLTTSGMKVGGGGGVALRIGKTAIYTFNFADGPDGFVFTLGDGWMF"
     gene            complement(561..1097)
                     /locus_tag="VFU45_01605"
     CDS             complement(561..1097)
                     /locus_tag="VFU45_01605"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU5357785.1"
                     /translation="MSAVAAPASLDFRALWGTAIPFSQFLDECAPEHLPLWQGIYRTA
                     SIPTWIATLDLAPSCRLLVITEDWCGDGANTVPVIQRWAEEEGIELRILRRDEHPQVM
                     DRYVLHGARSIPVVIALDADFRELGHWGPRPAELQAWVLAHKDSMDKDARYKETRRWY
                     ARDRGETTLREVAGMMVR"
     gene            complement(1187..2329)
                     /locus_tag="VFU45_01610"
     CDS             complement(1187..2329)
                     /locus_tag="VFU45_01610"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5357786.1"
                     /translation="MKMLVLGAGLQGCACAYDLLQVKAVTEVTLADLDPTRLPKFLAG
                     DHGGRLRTLKLDVTDGAALRAAIHGHESVMSALPYYLNEPIARAAVEIGAHFCDLGGN
                     TEIVLQQKKLNDAAIRQGVSVIPDCGLAPGMVNILAAEGIRRMDVAERVKIYVGGLPQ
                     KPEPPLNYQIVYSLEGALDYYTTPSLILRGGKKVEVDALSELEDVKFPKPVGTLEAFH
                     TAGGISTMPITFEKKVKVMEYKTLRYPGHVAVIRPIRELGLLDNKPIKVKGKDVVPRD
                     LFIAAVTPKLHKPEGKDLVALRVEVTGKRKGKKAREAWQLIDKYDAKHGISAMMRTTG
                     YSLAITGLMQADGRVQNHGVLTPDEAVPAGIYIKELAKRGVKIVEI"
     gene            2469..3092
                     /locus_tag="VFU45_01615"
     CDS             2469..3092
                     /locus_tag="VFU45_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5357787.1"
                     /translation="MTTPQNSPPRRPRDAEATRARLVRAALELFTTLGYRATTTALVA
                     GRAGIAEGTIYRHFGGKEELFLEVRRAAQRWGAELVRSTSEGGERPLPARERLRRVGR
                     GLLEAADREPALVRMALGLRHEPPPDDRGRDAAREFREALEQVIASGKSDGVIRAGPA
                     ELWSAVWLEVAGFAAEKVASREWPADHPSIGPVLEAAWDAIAPRSNP"
     gene            complement(3067..3852)
                     /locus_tag="VFU45_01620"
     CDS             complement(3067..3852)
                     /locus_tag="VFU45_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357788.1"
                     /translation="MTKSSLPVLALLALAACTQHFTPLQNRIAVGRQAYVVFAADGQG
                     GQGDLFAVDAAGGSPYPFTYSLADESHPALDPTGVMLAFIRSTSDTDRASRRVWVMDL
                     LNGRERHLGLPDGVVPDAVAWSRDGGTVFVATSTGDWALAAPPAASAPRRLTGADSLA
                     ADSALAVLVGDPPFARIGPCHPGADAICAFQGALTPTPVDSPARGPFRWGADSLGYFV
                     GERLMVRPVASGRPREVRVERVPDHPRDATWFRGLVRDSTGAR"
BASE COUNT          597 a         1294 c         1437 g          582 t
ORIGIN      
        1 tacaccacgg tgctgcgggg gtggaaggcg ttcggcgact acgccacctg gtggatcgac
       61 gggcgggtgc tggcctcgac caccagcggc acggtgccgc tcgacgccca gttcttcatc
      121 cctacctggg agcgctcgat ccggagttac ggcggagcgg agaccaaccg ggggctcgtg
      181 gaccagcgct acaccgggcc cgacctgctc gccgcgaccc tcgacgtcaa ccgcgacctc
      241 gtgaacgtag ggctctacgg cgcggtgagc gtccatgcct tcgtcgacgc cgggcgggta
      301 ttcgacaccg gcggattcca gctcacgacc agcgggatga aggtgggggg aggcggtggg
      361 gtggcgctcc ggatcggcaa gacggcgatc tacaccttca acttcgccga cggcccggac
      421 ggcttcgtgt tcacgctggg ggatgggtgg atgttctaag gaagaagtaa gaagtgaaaa
      481 gggaaaaggg ggctccgatg acgttcggag ccccctcttc actctcccct tttcacttct
      541 tacttctcac ttctcactta ctaccggacc atcatccccg ccacctcacg cagcgtcgtc
      601 tcgccgcggt cgcgggcgta ccagcgccgg gtctccttgt agcgggcgtc cttgtccatc
      661 gagtccttgt gcgccagcac ccaggcctgg agctccgccg ggcgggggcc ccagtgcccc
      721 agctcgcgga agtcggcgtc cagcgcgatg acgaccggga tcgatcgcgc cccgtggagg
      781 acgtagcgat ccatcacctg cgggtgctcg tcgcgccgga ggatacggag ctcgatcccc
      841 tcctcctccg cccaccgctg gatgaccggc accgtgttgg cgccgtcgcc acaccagtcc
      901 tccgtgatca cgagaaggcg gcacgagggc gcgaggtcga gggtcgcgat ccaggtcggg
      961 atcgaggcgg tgcgatagat cccctgccag aggggcaggt gttccggggc gcactcgtcg
     1021 aggaactggc tgaaggggat cgcggtgccc cagagggcgc ggaagtcgag tgacgcgggt
     1081 gcggcgacgg cggacatcaa atgtgcctca tttcggggac gagaaggcgc ggccaagcat
     1141 agaaagcaat acctgaccgc gccgtgaagt tcccgtgaag ccgcgttcag atctcgacga
     1201 tcttgacccc gcgcttggcg agttccttga tgtagatccc tgccggcaca gcctcatccg
     1261 gcgtcaggac accgtggttc tgcacccgcc catcggcctg catcaggccg gtgatggcga
     1321 gggagtagcc ggtggtccgc atcatcgcgc tgatgccgtg cttggcgtcg tacttgtcga
     1381 tgagctgcca cgcctcgcgc gccttcttcc ccttccgctt gccggtcacc tcgacccgca
     1441 gcgccacgag gtccttcccc tcgggcttgt ggagcttggg cgtcaccgcg gcgatgaaga
     1501 ggtcgcgcgg cacgacgtcc ttccccttca ccttgatggg cttgttgtcg aggaggccga
     1561 gctcgcggat ggggcggatg acggcaacgt ggcccggata gcggagggtc ttgtactcca
     1621 tcaccttgac cttcttctcg aaggtgatgg gcatcgtgct gatcccgccg gccgtgtgga
     1681 acgcctcgag ggtgccgacg ggcttcggga acttgacgtc ctcgagctcc gagagggcat
     1741 cgacctcgac cttcttcccg ccccgcagga tgagcgacgg cgtcgtgtag tagtcgagcg
     1801 caccctcgag cgagtagacg atctggtagt tgagcggcgg ctccggcttc tgcggcagcc
     1861 ccccgacgta gatcttgacc cgctccgcca cgtccatccg gcggatcccc tcggccgcga
     1921 ggatgttgac catccccggc gcgagcccgc agtccgggat gaccgaaacg ccctggcgga
     1981 tggcggcgtc gttcagcttc ttctgctgga ggacgatctc ggtgttgccg ccgaggtcgc
     2041 agaaatgggc gccgatctcg accgccgccc gggcgatcgg ctcgttgagg tagtacggca
     2101 gcgccgacat caccgactcg tgcccgtgga tcgcagcgcg gagcgcggcg ccgtcggtca
     2161 cgtcgagctt cagggtccgg agccggccgc cgtggtcgcc ggcgaggaac ttggggaggc
     2221 gagtggggtc caggtcggcg agcgtcactt ccgtgaccgc cttgacctgc aggagatcgt
     2281 acgcgcaggc gcagccctgc agaccggcgc caaggaccag catcttcatc gggactccag
     2341 gacaggatca gtgaggaaaa agcaccggaa tctagccggg cccgacccct cgttcaaccg
     2401 cggatcatcc tgcgtttgcg gcgccaggcg gagccggaaa gcgatgtccg acatagcttt
     2461 atgaagtgat gaccacgccg cagaactccc cacctcgccg cccccgggac gccgaagcga
     2521 cccgggcccg gctggtgcgc gccgccctgg agctcttcac cacgctcggg taccgcgcca
     2581 ccaccacggc gctcgtggcc ggccgggcag ggatcgccga gggaacgatc taccggcact
     2641 tcggcgggaa ggaggagctc ttcttggagg tgcgccgggc ggcgcagcgg tggggggcgg
     2701 agctggtccg gagcacgtcg gagggcggcg agcggccgct gcccgcccgc gagcggctgc
     2761 ggcgcgtcgg ccgcgggctc ctcgaggcgg ccgaccgcga gccggcgctg gtccggatgg
     2821 cgctgggcct gcggcacgag ccaccgcccg acgaccgggg cagggacgcc gcacgggagt
     2881 tccgggaggc gctggagcag gtgatcgcgt cgggcaagtc cgacggcgtg atccgcgcgg
     2941 ggccggcgga gctctggtcg gcggtgtggc tcgaagtggc gggcttcgcg gcggagaagg
     3001 tggcgtcacg ggagtggccg gcggaccacc cctccatcgg gccggtgctc gaagccgcgt
     3061 gggacgctat cgcgccccgg tcgaatccct gacgaggccg cggaaccagg tggcgtcacg
     3121 ggggtgatcc ggcacgcgct ccacccgcac ctcgcggggg cggcccgacg ccaccgggcg
     3181 caccatcagc cgctcaccga cgaagtagcc gagggaatcg gcgccccagc ggaaggggcc
     3241 gcgggcgggg gaatcgaccg gggtcggcgt cagtgcgccc tggaaggcgc agatggcgtc
     3301 ggctcccggg tggcagggcc cgatccgggc aaagggcggg tcgcccacca ggaccgcaag
     3361 cgccgaatcc gcagcgagcg agtcggcgcc ggtgaggcgg cgcggcgccg atgcggcggg
     3421 gggcgccgcc agggcccaat cgccggtgga cgtagcgacg aacaccgtgc caccgtcgcg
     3481 cgaccaggcc accgcgtcgg gcaccacgcc gtccgggagg cccaggtggc gttcccgtcc
     3541 gttgagcagg tccatcaccc acaccctccg cgacgcgcga tcggtgtcgc tcgtgctccg
     3601 gatgaaggcg agcatcaccc ccgtcgggtc gagggcggga tgggactcgt cggccagtga
     3661 gtaggtgaac gggtacgggc tcccgcccgc agcgtcgacc gcaaagaggt cgccctggcc
     3721 tccctggccg tcggccgcga agaccacgta ggcctgcctc ccgaccgcga tccggttctg
     3781 cagcggggtg aagtgctggg tgcaggcggc gagggcgagg agggcgagga cggggagcga
     3841 ggacttggtc atggggcgga aatgtagtgg cgaggagcgg gtcgcgagtg gcgggtgggg
     3901 ggcgggggac
//
LOCUS       DASUAP010000067        15708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1159917, whole genome shotgun sequence.
ACCESSION   DASUAP010000067 DASUAP010000000
VERSION     DASUAP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 15708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15708
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159917"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2388
                     /gene="polA"
                     /locus_tag="VFU45_01625"
     CDS             <1..2388
                     /gene="polA"
                     /locus_tag="VFU45_01625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU5357789.1"
                     /translation="GDKDFYQLIGPGIAVLNPGRGGAASVDPVWVDESNAQERLGVPP
                     AQVADYLALVGDSADNVPGVRGIGEKGAAELLRQYGDLETLLAHAGEIRQKRYREGLE
                     QHADLARLSRRLVTIRHDAPATLDLEAFAVRPADPDALARLYAELEFTSLLRQLGSAA
                     ATAATVTPARGPAAGAAAPSVPGVGPTVAVVDDPAALAGVIARLRQAPLVALDTETSS
                     LEPHSAELIGLSLAASATEVWYLPFGHRPAGGELAAPAPVRNLPPIGDPACRGVVDLL
                     RDPSVPKAGHNIKYDWQVLRRAGVELAGVAYDSMLASFVLDPGRRSHAIDVLSLEHLN
                     VPMRGYTDLTGKGKAQIPFAEVPVADAAAYCGHDSATVLALHALFAPRLEETGLAHLL
                     TDLELPLVPVLVDMEWTGIAIDRALFAKVSGELTHDLQELEARIAKAAGASVNLNSPK
                     QLGALLFEKLQLPVLKKTKTGASTDAEVLEELAGMGHEVPKLILEYREAQKLKSTYVD
                     ILPAAVHPATGRIHTSFHQTGAATGRLSSSDPNLQNIPVRTPRGEEIRRGFVPAPGCR
                     FVVADYSQIELRLLAHFSEDPAFIEAFHAGGDIHRGTAAVIFGVAEADVTPEMRARAK
                     TINFGTIYGQGPFALSRQLGISQEEARAFITQYFERFAGVRAWLDRMVELARAQGYVE
                     TLFGRRRYIPEIKDRNFSVRSFGERVATNSPLQGSAADLLKLGMIRLHAALHADGLGA
                     EILLQVHDELVVEAPAAEVERVERLVREKMEGAAQLRVPLVVSVGVGDNWLDAKH"
     gene            2474..3814
                     /locus_tag="VFU45_01630"
     CDS             2474..3814
                     /locus_tag="VFU45_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357790.1"
                     /translation="MPRASTLVAVALLCLAPFRALWAWQSPASDTGQARLRVFIDCQQ
                     TFCDDNFFRTELTWVDHVRTPEDAEVQVLITSQTSGGGGDAYTLVFLGHARFAGRQDT
                     LAYFAPRDNTDDATRKGLLQRIELGLVRYAADTRAADRLHVTFAPPAAGNPSRAPARD
                     PWDHWVFRVSGSTNLNGQQTSKFTYVSGSVSASRVTDRSKFRLSFNDSYNRSTFDFDV
                     VDSTYGDTTIFSHTEHSVSESKYFNGDALMVWSVGGRWSAGGEVTATRSTSLNETLAL
                     RVAPGLEYDLFPYSESAHRLLTFRWQVGLDRYVYDDTTIFDKTRETLLDQSFNVSFNA
                     VQAWGSAGLSIEAAQYLNDLGHNHLSVFGNASVRLVRGLSLNFFGSVASIHDQLYLSR
                     SGLSPVDVYLQRRQLATNYQYSLYTGLSYTFGSIFNNVVNPRFEGGGGGVFFFN"
     gene            complement(3830..6118)
                     /locus_tag="VFU45_01635"
     CDS             complement(3830..6118)
                     /locus_tag="VFU45_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU5357791.1"
                     /translation="MGSVAETLVRWRWGVILAWVVAGFFAFRAAPQTPALLNTRGGSR
                     HPTEASRADSLIRTRFPEPLSEYFAVTVQGPDRLTDLPGRTLIDSLSEASRRLPYVRD
                     VLGFGSTNDSMFASRDGRTAFFVVALRVPTSDSAGALVQPYRDALSRAFAAQPGHERY
                     TIQVTGRAPLDVDIRNVSAQDSARSEQRLLPLTLVILVLAFGALVAAVLPLVVGFLAI
                     AVTLAIVGVLAHHIPMSVFVLNMTSMIGLGVGIDYSLLVVTRFREELTRGLTRTQAAA
                     STLRTAGAAVITSGLTVVVGFAALLFTPLIETQSVGIGGLVVVVTAVLLATTLLPALL
                     AVFGRQIDRPRWLARRLAWYHAPAIWEQWARSLARHPRRALTLGGLGVALLTAPVVLI
                     HIGLPSRNWWPLATEAGRGVQTLQEIGAAGVILPVRVVVEFPTGRNAVDATSLRGLMQ
                     FSDSLRADGRVADVRSIVSLQRGGGSLLGYSMLYSDLPAARAQYGTYLDAYLSTDARV
                     TLLDVLVGDTTSLTATMDLVRRARALAAHPPKGLRGARILVGGYQAASVDFQDDLLAR
                     FPLMVLMILGSTGLMLAIAFRSLLVPVKAVIMNTLSVSATFGVIVLVFQYGYGSRVFG
                     LDGPADAIFVAVPVLVFAVVFGLSMDYEVFLLARIKEAFDTHGRNSQATMEGLSATAS
                     TITSAALIMILVFGVFAFARVLVVQFLGFGLAVAVLLDATLIRMVLVPAFMHLAGRWN
                     WWPGVRRRPVAPARPEPPTAGT"
     gene            6247..7011
                     /locus_tag="VFU45_01640"
     CDS             6247..7011
                     /locus_tag="VFU45_01640"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU5357792.1"
                     /translation="MPRVRVLAVVTLMLCAAVTADALHRAWMAHRGHQPAAAPPPPPV
                     HSSLSARAAPLPASAADTLSAGDRAAARRAISAAAGTVYFDSLFAETDSTLRRWPDSV
                     IGSLGIVMDGGGPPEWRPEMLDQVRDAMRGWESALPGLHFVQLLDTTGADITVHWVER
                     LGADRTGQADLVWDRAGRIHHVDVSLALRGPTGRTLSDFGLRAVAAHELGHALGLAHS
                     ADSTDVLYPATRTDTLSPRDVATVQLLYRLRPGGVK"
     gene            7011..8177
                     /locus_tag="VFU45_01645"
     CDS             7011..8177
                     /locus_tag="VFU45_01645"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5357793.1"
                     /translation="MRRTVRSAALLGALALASPPTVRAQSFAAADAAVAAGVRAGVYP
                     GAVLVVGTRDSVLHARGFGRLTWSRRSVPVSPDSTVWDLASLTKVVATTPAVMLLVDR
                     GLVDLDAPVARYLPRFTGAGKDRVTVRMLLDHTSGEPAWRPFSSLAPDRDSAIALLYA
                     TPLARTPGASAVYSDLNAMLLGLLVAQLTGTPLDAFATRDIFAPLGMHDTRFRPPGSW
                     RHRVAPTGRFHGRPVAGIVNDRNAERLGGVAGHAGLFSTGRDLARYAQFWLRGGTLPS
                     GTPLVSGATMARFLAPAARSGNRLLGWERPDPAEFSPDPYGSLLSDSAYGHTGWTGTQ
                     IWIDPARDLFVVLLTNRSYDPRVPKPFTALHAVRGQVSDAVVRARDVDVRRARP"
     gene            8223..8519
                     /locus_tag="VFU45_01650"
     CDS             8223..8519
                     /locus_tag="VFU45_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEU5357794.1"
                     /translation="MLTEETVRKALRGVKDPELNLNVVDIGLIYDIAVNDAGDVHVKM
                     SLTSPGCPSGPEITGDVERVIKDLEDVGTVEVELVWDPYWTPERMDPRIRAFLG"
     gene            complement(8626..9762)
                     /locus_tag="VFU45_01655"
     CDS             complement(8626..9762)
                     /locus_tag="VFU45_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEU5357795.1"
                     /translation="MPNSGTDLIATTTGLAELIARIGRPPLVALDTEAASFHRYSDAI
                     CLVQLSTRDVTAVIDPLAVPDLSGIGSLVADPAVEVVFHDADYDLRLLARDHAFHCTR
                     LFDTRVAAQLLNEPVIGLAALLEKHLGVSLDKKFQRADWSLRPLAPGMLEYAASDTRH
                     LPALRDRMRDLLVSAGRWTWAEEEFERQASVRWEPEDASMAFLRIKGSGKLRGRQLAV
                     LREVYNWREETARRLDRASFRVVNPDVLLALALAEPRDIEQLKGIKGISPGQAERYGG
                     EILAAVARACSLPEAEWPRIERGRRPPPDPEFDARLERLKAVRTAAAERIGLAPGVLC
                     PNGTLEAIARAAPVSMEALRQVPELRKWQAEVVGPELLAAVEKR"
     gene            complement(9752..9922)
                     /locus_tag="VFU45_01660"
     CDS             complement(9752..9922)
                     /locus_tag="VFU45_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357796.1"
                     /translation="MRPPTEYIRHAAKLARKLQMDAAMGGSAQPDAEFWKALGKALDE
                     AAAGLEMVSRAE"
     gene            10038..11246
                     /locus_tag="VFU45_01665"
     CDS             10038..11246
                     /locus_tag="VFU45_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5357797.1"
                     /translation="MPIPDIDLTVYPDDCDAFGHLNQAAFLSLFERARWEMLARGPGM
                     DVFTRANAWPAVRKTTIDYHAPAFPGDVLRFQQALTHHGQTSFTMRQTARRVADDSLI
                     ATAEFVFVCINREGRPVPVPVEVARFMNARPSSRGEMARLTVNGVNLAVDIRGDGPAI
                     LFVHGYPLDRTIWQHQVGALTGWRRIAPDLRGFGLSDAPDLGYSMATWADDLAALLDA
                     LGVEQAVLCGHSMGGYIGFEFARRHRARLRALVLVDSRAEADTAEGKRAREAAMVVAR
                     EQGPRAIAAQMLPKMLAPGAAQTMPQVADRVRGIMESAPLSGILGALGAMRDRPDSTP
                     LLPELAGVPTLAIVGEQDELTPPPMSRAIVDRVPGAVLGVIPNAGHLPPVEQPLATTR
                     VLGEFLDSLH"
     gene            complement(11470..11733)
                     /gene="fdxA"
                     /locus_tag="VFU45_01670"
     CDS             complement(11470..11733)
                     /gene="fdxA"
                     /locus_tag="VFU45_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU5357798.1"
                     /translation="MTYVITEACIGVKDRACVDVCPVDCIYEGEDQLYIHPDECIDCG
                     ACEPECPVTAIFPEEDVPANLTQYIEKNREAFNSETPPGRPKR"
     gene            11861..13918
                     /locus_tag="VFU45_01675"
     CDS             11861..13918
                     /locus_tag="VFU45_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5357799.1"
                     /translation="MRGITLSAAMLFIPATVVVAQAPCPRSGTALVLSGGGAKGLAHI
                     GVLRALDSLGIHPDFIVGSSMGAVIGALYASGRSPAEIDSLFRAIPSSALFSNDIPVM
                     PRALGVLKPLVAWEQTDHGLELQRSAVNDGPINAWLGAALLDGDLAARGDFCRLSTPF
                     YAVATDLARRKPVPLSTGDLALAVRASVAIPLVFQPIREGGRYLADGGLSANTPAIVA
                     RGLGADRLIISEMTEHDDDSVDFASPFTVANYLLTYLFRQPGDTAYPGDVLIRTDVTG
                     IGNLDFTVPILDSAEAHGYAATMAAFAGVPRTDGAGPAPRVAAPPPLLAEVSTPGLRA
                     TDARDLLRALSLTPGQPIDTARLRRGLLDLAQSDRFRGVWLHPDSGAPGDSLHLRLEV
                     QKAPSRTAALGLAYDNELGARLWVGAIDRHILDRAAEASAVAFFGGLETRVVLGLRRT
                     WKSNAIPRPTLMLTVIDQRIPTFDPGGDELAKLDAEQAVGFAGFERLFGAEWVVDAGL
                     EGRLWAEPAASRQRSGGIVFRVLQADPGREARFDLSLEWADRYRRASFEWQSPIPWGR
                     VTITPRLRAGIGDSLPLQLTFPLGGDDGFPGLHIYELRGDREAFGSLTIAVPIAGSLE
                     LRAEAATGQAAFGSGALALDNWHLGGRIGLGVSTPLGPARLEYGVTRDFRNQVLLRVG
                     RWF"
     gene            complement(14136..14708)
                     /locus_tag="VFU45_01680"
     CDS             complement(14136..14708)
                     /locus_tag="VFU45_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5357800.1"
                     /translation="MTVHRIRLLGDPVLRARCEPIAKPRSAAVRVVLDDLKDTLRDVR
                     RQVGFGRAIAAPQIGAPVRLVYVELDQPWPLLNPEIVDIGTEDFTVWDDCLSIPDLMV
                     RVVRAYRIKVRYQDVKGVEHSVDLEGDRAELLQHEIDHLDGVLPVDRPHGLDPFCYRA
                     EWERFYKDHGRYGAPEPRANHYATPLTGLL"
     gene            15013..>15708
                     /locus_tag="VFU45_01685"
     CDS             15013..>15708
                     /locus_tag="VFU45_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU5357801.1"
                     /translation="MSSSVESLVNQEYKYGFVTDIDADVFPPGLNEDVVRAISARKQE
                     PAFLLEWRLQAYRKWLTLREPHWANVTYGPIDYQAIRYFSAPKTKKPLGSLAEVDPEL
                     LRTYEKLGISLTEQKMLAGVAVDAIFDSVSVATTFKAKLKEYGIIFCSFSEAVREHPE
                     LVQKYLGTVVPSGDNFFAALNAAVFSDGSFVYIPKGVKCPMELSTYFRINTADVGQFE
                     RTLIVADEGAQVSY"
BASE COUNT         2295 a         5787 c         5265 g         2361 t
ORIGIN      
        1 ggcgacaagg atttctacca gctcatcggc cccggcatcg cggtgctcaa cccgggacgg
       61 ggcggggccg cgagcgtcga cccggtgtgg gtcgacgagt ccaacgcgca ggaacgcctg
      121 ggggtgccgc cggcccaggt ggcagactac ctggcgctcg tcggcgacag cgccgacaac
      181 gtccccgggg tccgcgggat cggcgagaag ggcgccgcgg aactcctccg ccagtacggc
      241 gacctcgaga cgctgctggc ccacgccggc gagatcaggc agaagcgcta ccgcgagggg
      301 ctggagcagc acgccgacct cgcgaggctc tcccgccgcc tcgtcaccat ccggcacgat
      361 gcgcccgcca cgctcgacct cgaggcgttc gcggtccgcc ccgccgatcc cgacgccctc
      421 gcgcggctct acgccgagct cgagttcacc tcgctcctcc ggcagctcgg ctccgccgcg
      481 gccacggccg cgacggtcac gcccgcccgc ggccccgccg ccggcgccgc ggccccgtcg
      541 gtgcccggcg tcggcccgac cgtggcggtg gtggacgatc cggccgcgct cgccggggtc
      601 atcgcccggc tgcgacaggc gccgctcgtg gcgctcgata ccgagacgag ctcgctcgag
      661 ccgcacagcg ccgagctgat cgggctctcg ctggccgcat cggccaccga ggtgtggtac
      721 ctcccctttg gccaccgtcc ggccgggggc gagctcgccg cccccgcccc ggtcaggaac
      781 ctccccccga tcggcgatcc cgcctgccgc ggggtcgtcg acctcctccg cgacccgtcg
      841 gtgcccaagg ccgggcacaa catcaagtac gactggcagg tgctgcgccg ggccggggtg
      901 gagctggcgg gcgtggccta cgactcgatg ctcgcgagct tcgtgctcga tccgggccgc
      961 cgctcccacg ccatcgacgt gctgagcctc gagcacctga acgtgccgat gcggggctac
     1021 accgacctca ccggcaaggg gaaggcgcag atccccttcg ctgaggtccc ggtcgcggac
     1081 gcggccgcct actgcggcca cgacagcgcc acggtcctcg ccctgcacgc gctcttcgcc
     1141 ccgcgcctgg aggagacggg gctcgcccac ctgctcaccg acctcgagct cccgctggtg
     1201 ccggtgctgg tggacatgga atggaccggg atcgccatcg accgggcgct ctttgccaag
     1261 gtctccggcg agctgaccca cgacctgcag gaactcgagg cgcggatcgc gaaggccgcg
     1321 ggcgcgagcg tcaacctcaa ctcgccgaag cagctcggcg cgctcctgtt cgagaagctg
     1381 cagctgccgg tcctcaagaa gaccaagacc ggcgcctcca ccgacgccga ggtgctcgag
     1441 gagctggcgg ggatgggcca cgaggtgccg aagctcatcc tcgagtaccg cgaggcgcag
     1501 aagctcaaga gcacctacgt cgacatcctc cccgccgccg tgcacccggc caccggccgg
     1561 atccacacga gctttcacca gacgggggcg gccaccggcc ggctctcctc gtccgacccc
     1621 aacctccaga acatccccgt gcgcacgccg cgcggggagg agatccgccg cgggttcgtc
     1681 cccgcgccgg ggtgccggtt cgtcgtggcc gactacagtc agatcgagct ccggctgctg
     1741 gcgcatttct cggaggatcc cgcgttcatc gaggcgttcc acgccggcgg ggacatccac
     1801 cgcggcaccg ccgcggtgat cttcggcgtc gccgaggcgg acgtgacgcc cgagatgcgc
     1861 gcccgggcca agacgatcaa cttcgggacg atctacggac agggtccctt cgcgctctcc
     1921 cgacagctcg ggatctcgca ggaggaggcc agggccttca tcacccagta tttcgagcgc
     1981 ttcgccgggg tccgcgcgtg gctcgaccgg atggtggagc tcgcgcgcgc gcaggggtat
     2041 gtcgagacgc tcttcgggcg ccggcgctac atccccgaga tcaaggaccg caacttcagc
     2101 gtccgttcct tcggcgagcg ggtggccacc aactccccgc tccagggctc cgccgccgac
     2161 cttctcaagc tcggcatgat ccgcctccac gccgccctcc acgccgacgg gctcggcgcc
     2221 gagatcctcc tccaggtgca cgacgagctg gtggtcgagg cgcctgccgc cgaggtggag
     2281 cgggtcgagc gcctggtgcg cgagaagatg gaaggcgccg cccagttgcg ggtgccgctg
     2341 gtggtgtccg tcggcgtcgg cgacaactgg ctcgacgcca agcactgagc gccgcgcaac
     2401 cattccgggg cgggcggcat ccgtatcccg ggtgttcggc ccccttcccc ttccctgtcg
     2461 gtcaggagct tcgatgcctc gcgcgtccac gctggtggcg gtggccctgc tgtgcctcgc
     2521 cccgttccgc gccctctggg cctggcaaag ccccgcctcc gacaccggac aggcccgcct
     2581 ccgggtcttc atcgactgcc agcagacctt ctgcgacgac aacttcttcc gcaccgagct
     2641 cacctgggtg gaccacgttc ggacgccgga ggacgccgag gtgcaggtgc tgatcacctc
     2701 ccagacgagc ggcggcggcg gggacgcgta taccctcgtc ttcctgggcc atgcccggtt
     2761 cgccgggcgc caggacaccc tggcctactt cgcaccgcgg gacaacaccg acgacgccac
     2821 ccggaagggc ctgctgcagc gcatcgagct ggggctcgtc cgatacgccg cggacacccg
     2881 ggccgccgac cggctgcacg tgacgttcgc gccgccggcg gccgggaacc cgtcccgcgc
     2941 gccggcacgc gacccgtggg atcactgggt gttccgggtg agcggcagca ccaacctcaa
     3001 cggccagcag accagcaagt tcacctacgt cagcgggtcg gtctccgcca gccgggtcac
     3061 cgaccggtcg aagttccgcc tgtcgttcaa cgacagctac aaccggagca ccttcgactt
     3121 cgacgtcgtc gatagcacct acggcgacac cacgatcttc agccacaccg agcacagcgt
     3181 cagcgagtcg aagtacttca acggggacgc actgatggtg tggagcgtgg gcgggcgctg
     3241 gtcggcgggg ggtgaggtga cggccacacg gtccacgtcc ctcaacgaga ccctggcgtt
     3301 gcgcgtggcg ccaggactgg aatacgacct cttcccctat tcggagtcgg cccaccggct
     3361 gctgacgttc cgctggcagg tcggcctgga ccggtacgtg tacgacgaca cgaccatctt
     3421 cgacaagacg cgggagacgc tcctggacca gtcgttcaac gtgtcgttca atgcggtgca
     3481 ggcgtgggga tcggcggggc tgtccatcga ggcggcacaa tatctcaacg acctggggca
     3541 caaccacctg tcagtgttcg gcaatgcgag cgtgcggctg gtgcgcgggc tctcgctcaa
     3601 tttcttcggc agtgtggcgt ccatccacga ccagctctac ctctcccgca gcggcctgag
     3661 cccggtggac gtctacctcc agcgacggca gctggccacc aactaccagt attccctcta
     3721 caccggcctc agctacacct tcggctcgat cttcaacaac gtggtgaatc cgcggttcga
     3781 agggggcggg ggcggcgtgt tcttcttcaa ctgacgccgt gctggccgat caggtcccgg
     3841 cggtgggcgg ctcgggccgg gcgggcgcca ccgggcgccg gcgcaccccc ggccaccagt
     3901 tccagcgccc ggcgaggtgc atgaaggccg ggaccagcac catgcggatg agtgtcgcgt
     3961 cgaggagcac cgccaccgcc aggccgaagc cgaggaactg caccaccagc acccgcgcaa
     4021 aggcgaacac gccgaacacc aggatcatga tgagcgcggc cgacgtgatc gtcgacgcgg
     4081 tggcggagag tccctccatc gtggcctggg agttccggcc gtgggtgtcg aaggcctcct
     4141 tgatccgcgc cagcaggaac acctcgtagt ccatcgacag cccgaacacc accgcgaaga
     4201 cgagcaccgg caccgcgacg aaaatcgcgt cggccgggcc gtcgagtccg aagacccgcg
     4261 agccgtagcc gtactggaac acgagcacga tgacgccgaa cgtcgcactc accgagaggg
     4321 tgttcatgat caccgccttg accggcacca ggagcgaccg gaacgcgatc gccagcatca
     4381 gcccggtgga gccgaggatc atgagcacca tcagcgggaa gcgggccagc aggtcgtcct
     4441 ggaagtccac gctcgcggcc tggtagccgc cgaccaggat gcgcgcgccg cggagcccct
     4501 tgggcgggtg ggcggccagc gcgcgcgccc ggcgcaccag gtccatcgtc gccgtgagcg
     4561 aggtggtgtc acccacgagc acgtcgagga gcgtcacccg cgcgtcggtg ctcaggtagg
     4621 cgtcgaggta ggtgccgtac tgcgcccggg ccgcggggag gtcgctgtag agcatcgagt
     4681 agccgaggag cgagccgccg ccgcgctgca gcgacacgat gctccgcacg tcggccaccc
     4741 gtccgtcggc gcggagcgag tcgctgaact gcatcaggcc gcggaggctc gtcgcgtcga
     4801 cggcgttgcg ccctgtgggg aactcgacca cgacccgcac cggcaggatc acgcccgccg
     4861 cgccgatctc ctgcagcgtc tgcaccccgc gtcccgcctc ggtggcgagc ggccaccagt
     4921 tccgggacgg cagcccgatg tggatgagca ccaccggggc ggtgaggagt gccacgccga
     4981 gcccgccgag cgtgagcgcg cggcgcgggt ggcgtgccag gctccgggcc cactgctccc
     5041 agatggcggg cgcgtggtac caggccagcc gccgcgccag ccagcggggc cggtcgatct
     5101 ggcgcccgaa cacggcgagc agcgccggca gcagcgtggt ggccaggagc accgcggtca
     5161 ccaccaccac gagcccgccg atgccgacgc tctgcgtctc gatgagcggg gtgaagagga
     5221 gcgccgcaaa gcccaccacc acggtgagtc ccgacgtgat gacggcggcc cccgcggtcc
     5281 ggagcgtgga ggccgcggcc tgggtgcggg tgagcccgcg cgtcagctct tcccggaacc
     5341 gggtcacgac cagcagcgag tagtcgatgc cgacgccgag cccgatcatc gacgtcatgt
     5401 tgaggacgaa caccgacatc gggatgtggt gcgccagcac gcccacgatc gccagcgtca
     5461 ccgcgatggc caggaaaccg accaccagcg ggagcaccgc cgccaccagc gccccgaagg
     5521 ccagcaccag gatgacgagc gtgagcggca ggagccgctg ctcgctgcgg gcgctgtcct
     5581 gcgcgctcac gttgcggatg tcgacgtcga gcggcgcgcg cccggtgacc tggatggtgt
     5641 accgctcgtg gcccggctgt gcggcgaacg cccgcgacag cgcgtcgcgg tacggctgca
     5701 ccagtgcgcc cgcgctgtcc gaggtcggta cccggagcgc gacgacgaag aacgcggtcc
     5761 gcccgtcgcg gctcgcgaac atcgagtcgt tggtcgagcc gaagccgagc acgtcccgca
     5821 cgtacggcag ccggcgcgat gcctcggaca gggaatcgat cagcgtccgg cccggcaggt
     5881 cggtgagccg gtcggggccc tgcacggtga cggcgaagta ttcgctgagg ggctcgggaa
     5941 agcgcgtgcg gatgagcgag tcggcccggg aggcctcggt cgggtggcgg ctgcccccgc
     6001 gggtgttgag gagcgccggc gtctggggcg cggcgcggaa cgcgaagaac cccgccacga
     6061 cccaggcgag gatcacgccc caccgccacc ggaccaacgt ctccgccacg gatcccatcg
     6121 accggatgcc tcctcgtgcg cgccgggtgc tccgccggcc gcccggaggc gctgcggagg
     6181 cccaatgtta ggcgcggccg gcccggggcg ctagcggtta tgcgggccgc ggccgtattc
     6241 tccggggtgc cccgagtccg cgtcctggcc gtcgtcaccc tgatgctctg tgcagccgtc
     6301 accgccgacg cgctgcaccg cgcgtggatg gcgcaccgcg gccaccagcc tgccgccgca
     6361 ccgccgccgc cgccggtcca ttcctccctg tccgcccgcg ccgcccccct cccggcgtcc
     6421 gctgccgaca ccctgagcgc cggcgaccgc gccgccgcgc gccgcgccat ttccgcggcg
     6481 gccggcacgg tgtacttcga ttcgctcttc gccgagaccg actccaccct ccgccgctgg
     6541 cccgattcgg tcatcggttc gctcggcatc gtgatggacg gcggcggccc gccggagtgg
     6601 cgccccgaga tgctcgacca ggtgcgcgac gcgatgcggg gctgggaatc ggcgctcccg
     6661 gggctccact tcgtccagct cctcgacacc accggcgccg acatcaccgt ccactgggtc
     6721 gagcgcctcg gcgccgaccg caccggccag gccgacctcg tctgggaccg cgccggccgc
     6781 atccaccacg tcgacgtgtc gctcgccctg cgcggcccga ccggccggac gctgagcgac
     6841 ttcggcctgc gcgccgtcgc cgcccacgaa ctcgggcacg cgctcggcct ggcgcattcg
     6901 gccgactcca ctgacgtgct ctacccggcc acgcgcaccg ataccctctc accccgcgat
     6961 gtggccacgg tgcagctgct gtatcgtttg cgccccggcg gagtcaagtg atgcgccgca
     7021 ccgtccggtc cgcggcgctg ctgggcgcgc tcgccctggc ctccccgccc accgtccgcg
     7081 cccagtcctt cgcggcggcg gatgcggcgg tggccgcggg cgtgcgggcc ggcgtgtatc
     7141 cgggcgccgt cctcgtcgtc ggcacccgcg acagcgtgct ccacgcccgg ggtttcggcc
     7201 gcctcacctg gagccggcgc tcggtcccgg tatcgcccga ctccacggtg tgggacctcg
     7261 cctcgctcac caaggtggtg gcgacgacgc cggcggtgat gctcctcgtc gaccgcggcc
     7321 tggtcgacct cgacgcaccg gtggcccggt acctgccgcg attcaccggc gccggcaagg
     7381 accgggtcac cgtgcggatg ctcctcgacc acacgagcgg cgagccggcg tggcgaccgt
     7441 tctcgtccct cgcccccgac cgcgacagcg ccatcgcgct cctctacgcg accccgctcg
     7501 cccggacccc cggcgcgtcc gcggtctaca gcgatctcaa cgcgatgctc ctgggcctcc
     7561 tggtggcgca gctcactggt acgccgctcg atgcattcgc gactcgcgac atctttgccc
     7621 ccctcgggat gcacgacacc cggttccgcc ccccgggatc ctggcgccac cgtgtcgcgc
     7681 cgacaggccg gttccacggc cgcccggtcg ccggcatcgt caacgaccgg aacgccgagc
     7741 gcctcggcgg cgtggccggc cacgccgggc tcttttccac cggccgcgac ctcgcacgct
     7801 acgcccagtt ctggctccgc ggcgggacgc tgccgtcggg gacgccgctc gtttccggcg
     7861 cgacgatggc tcgcttcctt gcccctgcgg cccggagcgg caaccgcctc ctcggctggg
     7921 aacggcccga tccggcggag ttctcccccg atccctacgg atcgctcctc tccgactcgg
     7981 cgtacggcca caccggctgg accggcaccc agatctggat cgacccggcg cgggatctct
     8041 tcgtcgtgct cctcaccaac cgcagctacg acccgcgggt gccgaagccg ttcaccgcgc
     8101 tccacgccgt ccgcgggcag gtgtccgacg cggtggtgcg cgcacgggac gttgatgtgc
     8161 gccgtgcgcg cccctaagtt gcggggagcc ggccgtgtgt cggcgctcca gggaggaatg
     8221 tgatgctgac cgaagagacc gtccgcaagg cgttgcgcgg cgtgaaggac ccggaactca
     8281 acctcaacgt cgtcgacatc gggctgatct acgacatcgc ggtcaacgac gcgggcgacg
     8341 tccacgtcaa gatgtcgctc acctcgcccg ggtgcccgtc ggggcccgag atcacgggcg
     8401 acgtcgaacg ggtgatcaag gacctcgagg acgtggggac ggtggaggtg gagctggtct
     8461 gggatccgta ctggaccccc gaacggatgg atccgcggat ccgggcgttc ctggggtgag
     8521 acgtgagagg tgagacgtga gaggtgagac gtaagaggtg agcggtgagt ggtaagaggg
     8581 cccccagtcg agatctcgat cgggggccct ctcacgtctc acctctcacc tcttctccac
     8641 cgccgccaac agctccggcc cgacgacctc ggcctgccat ttccgcaact ccggcacctg
     8701 ccgcagcgcc tccatcgaga ccggcgccgc tcgcgcgatc gcctccagcg tgccgttcgg
     8761 gcagagcacc cccggcgcca gtccgatccg ttccgcggcc gcggtgcgga ccgccttgag
     8821 ccgctcgagc ctcgcgtcga actccggatc gggaggcggg cgcctgcccc gctcgatccg
     8881 gggccactcc gcctccggca gcgagcatgc ccgggccacc gcggcgagga tctccccgcc
     8941 gtagcgctcc gcctgccccg gcgagatccc cttgatcccc ttcagctgtt cgatgtcgcg
     9001 cggctcggcc agcgccagcg ccagcagcac gtcggggttg accacccgga acgatgcccg
     9061 gtcgagccgg cgggcggtct cttcccgcca gttgtacacc tcccggagca ccgccagttg
     9121 gcgccctcgg agcttccccg accccttgat ccggaggaag gccatcgacg catcctccgg
     9181 ttcccagcgc accgacgcct ggcgctcgaa ctcctcctcc gcccacgtcc accgaccggc
     9241 gctgaccaga aggtcgcgca tccggtcgcg cagggccggg aggtggcgcg tgtccgatgc
     9301 ggcgtactcg agcatccccg gtgcgagcgg gcggagggac cagtcggccc gctggaactt
     9361 cttgtccagg gacacgccga ggtgcttctc gaggagcgcc gccaggccga tcaccggctc
     9421 gttgaggagc tgtgctgcga cccgggtgtc gaagaggcgg gtgcagtgga acgcgtggtc
     9481 gcgcgccagg agccgaaggt cgtagtcggc gtcgtggaag accacctcga ccgcggggtc
     9541 ggccaccagc gagccgatgc cgctcaagtc gggcaccgcc agcgggtcga tgaccgcggt
     9601 gacgtcgcgg gtcgagagct ggaccaggca gatcgcgtcg gagtagcggt ggaagctcgc
     9661 cgcctcggtg tcgagcgcca cgagtggtgg ccggccgatc cgcgcgatca gctcggccag
     9721 ccccgtcgtg gtcgcgatca ggtcggtgcc gctattcggc acgcgacacc atctccaggc
     9781 cggcggcggc ctcgtcgagg gccttgccca gggccttcca gaactcggcg tccggctggg
     9841 cgctcccgcc catcgcggcg tccatctgga gcttgcgggc cagcttggcg gcgtggcgga
     9901 tgtactcggt cggtgggcgc atgcggcgat cctccctggg gacggcgtct atcccggacg
     9961 gcgggcacgc catataatct cagtgcaagc tagccggctg gccggcgccg aagaatgcac
    10021 cgatcatcgg atccctgatg cccatccccg acatcgacct caccgtctat cccgacgact
    10081 gcgatgcgtt cggccacctg aaccaggcgg cgttcctgtc actcttcgag cgggcccggt
    10141 gggagatgct cgcccgcggc ccgggcatgg acgtgttcac ccgcgccaac gcctggcccg
    10201 ccgtgcggaa gacgaccatc gactaccacg ctcccgcctt tccgggcgac gtcctgcgct
    10261 tccagcaggc cctcacccac cacggccaga ccagcttcac gatgcgccag acggcgcggc
    10321 gcgtggccga cgactcgctc atcgccactg cggaattcgt ctttgtctgc atcaatcgcg
    10381 aaggccgtcc ggtccccgtg ccggtcgagg tcgcgaggtt catgaatgcg cgcccgtcga
    10441 gccgcgggga gatggcccgc ctcacggtca acggcgttaa cctcgccgtc gacatccgcg
    10501 gcgatggccc cgccatcctc ttcgtgcacg gatacccgct cgaccgcacc atctggcagc
    10561 accaggtggg cgcgctcacc gggtggcggc gcatcgcgcc cgacctgcgc ggcttcggcc
    10621 tctccgacgc gcccgacctc ggctactcga tggccacctg ggccgacgac ctcgcggcgc
    10681 tcctcgacgc gctcggggtc gagcaggcgg tgctctgcgg tcactcgatg ggcgggtaca
    10741 tcggcttcga attcgcccgg cgccaccgtg cccggttgcg ggcgctcgtg ctggtggaca
    10801 gccgtgccga ggccgatacg gccgaaggca agcgcgcgcg ggaggccgca atggtggtgg
    10861 cccgggaaca gggcccccgc gccatcgctg cgcagatgct cccgaagatg ctcgcgcccg
    10921 gcgccgccca gacgatgccg caggtggccg accgggtccg gggcatcatg gagtcggccc
    10981 cgttgagcgg gatcttgggc gccctcggcg cgatgcgcga ccgccccgac agcaccccct
    11041 tgctccccga gctcgccggc gtccccaccc tcgcgatcgt cggggaacag gacgagctca
    11101 cgccaccgcc gatgtccagg gccatcgtcg accgggtccc cggcgccgtg ctcggcgtga
    11161 tccccaacgc cggccacctc cccccggtcg aacaacccct cgccaccacc cgcgtgctgg
    11221 gcgagttcct cgactcgctg cactgaaacc atccggccgg ctcgggcgtc tatatgtgtg
    11281 gcggtcggac cctactccct cgggtccacg ccgatcgaag tcggtagttc ctccagttcg
    11341 ggccccgggg tcgcggccaa ggcagtacga tcccggggcc tgccacgtcc gccccctcgt
    11401 gttccaagcc gaaatgcaca gaccccccgc ccgcgtgggc gaggggcctc ggatgatcca
    11461 tgggaccggc tatcgctttg ggcgccccgg cggcgtctcc gagttgaagg cctcccggtt
    11521 cttctcgatg tactgcgtca ggttggccgg tacatcctcc tcggggaaga tggccgtcac
    11581 cgggcactcg ggttcacacg cgccgcagtc gatgcattcg tcggggtgga tgtaaagctg
    11641 gtcctctcct tcatagatgc agtcgacggg gcagacatcg acgcaggcgc gatccttcac
    11701 gccgatgcag gcctccgtga tgacgtacgt catttccgcc tgacctcctg cctgaccggt
    11761 ttccagttct ccgcgacgac gctcataagt atgagggggc cgcgctctac gcaaggggag
    11821 cggatgctct cgaccgagcg gcggcgctgt atcttccggc atgcgcggca tcaccctctc
    11881 tgcagcgatg ctgttcatcc cggccacggt cgtcgtggcc caggcgcctt gtccccggag
    11941 cggcacggcg ctcgtgctct cgggaggcgg ggccaaggga ctggcgcaca tcggggtgct
    12001 ccgcgcgctc gacagcctcg gtatccatcc cgacttcatc gtcggctcga gcatgggtgc
    12061 ggtcatcggc gcactctacg ccagcggccg ctcccccgcg gagatcgatt ccctcttccg
    12121 cgcgatcccg tcgtcggcgc tcttcagcaa cgacatcccc gtcatgcccc gcgcgctcgg
    12181 cgtcctcaag ccgctcgtgg cgtgggagca gaccgaccac ggactcgagc tccagcgctc
    12241 ggccgtcaac gacggaccga tcaacgcgtg gctcggcgcc gcactgctcg acggcgacct
    12301 ggccgcccgc ggcgacttct gccgcctcag caccccgttc tacgccgtcg ccaccgacct
    12361 ggcccgccgc aagccggtgc ccctcagcac cggcgacctg gcccttgcgg tccgggcgag
    12421 cgtggccatc ccgctggtct tccagccgat ccgcgagggc gggcgctacc tggccgacgg
    12481 cgggctctcg gccaacacgc cggccatcgt ggcgcgcggg ctcggcgccg accgcctgat
    12541 catctcggag atgaccgagc acgacgatga ttcggtcgac ttcgcctcgc cattcacggt
    12601 cgcgaattac ctgctcacct acctgttccg ccagccgggg gacacggcct acccgggcga
    12661 cgtgctgatc cgcaccgacg tcaccggcat cgggaacctc gacttcacgg tgcccatcct
    12721 cgactcggcg gaggcgcacg gctacgccgc gacgatggcc gcgttcgccg gtgtaccccg
    12781 gaccgatggg gcggggccgg ccccgcgggt ggccgcacca ccgccgctgc tcgcggaagt
    12841 cagcacgccg ggattgcggg ccaccgatgc ccgcgacctc ctccgggccc tctcgctcac
    12901 gcccggccag ccgatcgaca ccgcccggct ccgtcgcggg ctcctcgacc tcgcccagtc
    12961 cgatcggttc cgcggcgtct ggctccatcc tgactcgggc gccccgggag actcgttgca
    13021 cctgcggctc gaggtgcaga aggcgccgag ccggacggcc gccctcgggc tcgcgtacga
    13081 caacgaactc ggagcgcgcc tgtgggtcgg cgccatcgac cggcacatcc tcgatcgcgc
    13141 cgccgaagcg agcgccgtcg cgttctttgg cgggcttgag acgcgggtgg tgcttggact
    13201 ccggcgcacc tggaagagca acgccattcc ccgccccacg ctgatgctca ccgtcatcga
    13261 ccagcggatc ccgacgttcg acccgggtgg cgatgagctg gccaagctcg acgccgagca
    13321 ggcggtcgga tttgccggct tcgagcggct cttcggcgcg gaatgggtgg tggacgcggg
    13381 gttggaaggg aggctctggg ctgaaccggc cgcgtcacgc cagcgctccg gcgggatcgt
    13441 cttccgggtg ctgcaggccg atcccggccg cgaggcgcgg ttcgacctct cgctggaatg
    13501 ggccgaccgc tatcggcgcg cttcattcga atggcagtcg ccgatcccgt ggggccgcgt
    13561 caccatcact ccgcgtcttc gggccggcat cggcgattcg ttgccgctgc agctcacctt
    13621 cccgctcgga ggggacgacg gctttccggg cctccacatc tatgagctgc gcggtgatcg
    13681 cgaggcattc gggtcgctca ccatcgccgt cccgatcgct ggctcacttg agctccgcgc
    13741 cgaggccgcc accggccagg cggcgttcgg cagcggggcg ttggcgctcg acaactggca
    13801 tctgggggga cggatcggac ttggcgtcag caccccgctg ggtccggccc gcctggaata
    13861 cggcgtcacc cgcgacttcc gcaaccaggt gttactgcgc gtggggcggt ggttctaagt
    13921 aagaagtaag aagtaagaag tgagacgtga gaaggacggg gtaagacgtg gaacgtaaga
    13981 tgtgagaaac gagagaatag aactcaaacg gggtctccga atccgtggag accccttctg
    14041 acttctcatg cactccttct cacttcttgc ttctcacttc ttacgtctca cttctcactt
    14101 ctcacttctt acttctcact tctcacttct cactttcaca acagccccgt cagcggcgtc
    14161 gcatagtggt tggcccgcgg ttcaggggcg ccgtagcggc cgtgatcctt gtagaagcgc
    14221 tcccactccg cgcgatagca gaacgggtcg aggccatggg gccggtccac gggcagcacg
    14281 ccgtcgagat ggtcgatctc gtgctggagc agttcggcgc ggtcgccctc gaggtccacc
    14341 gagtgctcga cacccttcac gtcctgatac cggaccttga tccggtaggc ccgcaccacc
    14401 cgcaccatca ggtcggggat cgagaggcag tcgtcccaca ccgtgaagtc ctccgtgccg
    14461 atgtcgacga tctccgggtt gaggaggggc cacggctgat ccagctcgac gtacaccagc
    14521 cgcaccgggg ccccgatctg cggggccgcg atggcgcgcc cgaagccgac ctgccgccgg
    14581 acgtcgcgca gggtgtcctt caggtcgtcc agcaccacgc gcaccgcggc cgatcgcggc
    14641 ttggcgatcg gttcgcaccg ggcacggagc accggatcgc ccaggaggcg gatgcggtgt
    14701 acggtcatcg tccatccctt cggaggatct tcattggggc caatatacgg gttatcaatg
    14761 aattcgcacg cccttaaagt agtagctagt cagactacgt tatttggcag tgattggcta
    14821 cgaccgatga gcccgctgat gtcgcgtatg gccggccgcg tggtcaacgc ccgtcgcctg
    14881 cgcattaaac cattcacacg tcagcgatta gccgacagct ccgatatcca ccgccggttg
    14941 attgacaatt ccgacttcgg aacataggat tctttgttca cggtccggaa gcgtcgtcgg
    15001 aggcaggtcg ccatgagttc atcggtcgag tccctggtca accaggaata caagtacggc
    15061 ttcgtcaccg acatcgacgc ggacgtcttc ccgccgggcc tcaacgaaga tgtcgtccgg
    15121 gccatttccg cccggaagca ggagccggcg ttcctcctcg agtggcgcct gcaggcctac
    15181 cggaagtggc tcaccctccg cgagccgcac tgggccaacg tgacctacgg cccgatcgac
    15241 taccaggcca tccgctattt ctcggccccg aagaccaaga agccgctggg ctccctggcc
    15301 gaggtcgacc ccgagctgct gcgtacctac gagaagctgg gcatctcgct caccgagcag
    15361 aagatgctcg ccggcgtggc cgtggatgcc attttcgaca gtgtgtcggt ggccacgacg
    15421 ttcaaggcga agctcaagga gtacgggatc atcttctgct ccttctccga ggcggtgcgc
    15481 gagcacccgg agctggtgca gaagtatctg ggcacggtgg tcccctccgg cgacaacttc
    15541 ttcgccgccc tcaacgccgc ggtgttcagc gacgggtcgt tcgtctacat cccgaagggc
    15601 gtgaagtgcc cgatggagct gtcgacctac ttccgcatca acaccgcgga tgtcgggcag
    15661 ttcgagcgca ccctgatcgt ggccgacgaa ggcgcccagg tgagctac
//
LOCUS       DASUAP010000068        11946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1146227, whole genome shotgun sequence.
ACCESSION   DASUAP010000068 DASUAP010000000
VERSION     DASUAP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11946
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146227"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFU45_01690"
     CDS             complement(<1..216)
                     /locus_tag="VFU45_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU5357802.1"
                     /translation="MSPRIGITGRVRRSGGEFHAGVNAAYVAAVAAAGGIALVLPPTT
                     PASSAGAVAAALEGLIISGGADIDPARY"
     gene            complement(213..689)
                     /locus_tag="VFU45_01695"
     CDS             complement(213..689)
                     /locus_tag="VFU45_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357803.1"
                     /translation="MRRTLLLLVGLAGCGRPAGGSVTCGLAAMFGPLAILGEFSTPGQ
                     TLATPPGSLPGKLPVRLVAGPALPALVGRDHDRWVIGVIGAPPPKFRAGYGVLVQDTA
                     YRALGVVVYEGEIVRGAPVIGSVVLADSTVPLIGISLLPAKVEDPHCPIFPDSTVR"
     gene            825..1400
                     /locus_tag="VFU45_01700"
     CDS             825..1400
                     /locus_tag="VFU45_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357804.1"
                     /translation="MGFFARLRDRLDRLIDGATVPPTPRESAAALQQALIDLKVGLGD
                     LRRARESTERELAEERRQLADAERRGALAAAIPDPETVRVAQEFAGRHRERVAVLERK
                     LAVQMDEVALAEREAAELVERWREARRGLGPSGMTPSVEEAWRGLEAAGADRPELDLE
                     HDLLRHQADRAARDQAADLQLQALKKKLGKE"
     gene            1404..2984
                     /locus_tag="VFU45_01705"
     CDS             1404..2984
                     /locus_tag="VFU45_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEU5357805.1"
                     /translation="MSDQRSLLPDHPRTLTDHQEHRVASNSSDTAFFGHPRGLATLFG
                     TEMWERFSFYGLRAVVILYMTAALENGGLGWSVGKAGAIYGMYAALVYMLSLPGGWVA
                     DKLIGLRRAVLYGGILIMLGQLTLAIPGAAFLFPGLGLIILGTGLLKPNVSVIVGELY
                     PEKDLRRDAGFSVFYMGINLGATIAPLIVGGLAQAPGFRSLLTSWGFNPVNAWHWGFG
                     VGALGMLAGVIQFRLGWKHLGTAGAAPSVARDSAEGRSAWRIARLSVVAAVAVVVGLV
                     FLVRGGTWTAEGVAAKFPYLFGVLVVAFFVWLFGMGNWTRDERKRLVVVLVLFVASAI
                     FWAAFEQAPSTLNLFALQNTRNSIFGWTFPSTWLQSVNSLCIIALAPVFAWIWIRLGT
                     RDPSSPTKFGFGLLFAGLGFLVMMFAARASAGGVLVSPMWLVVTYLLHTVGELCLSPV
                     GLSSMTKLAPDRVKGMIMGVWFLGTSLGNLIAGNVTRLYGSLTVPQLFGAVAAFCIVP
                     AIIMFLLVVPIRRMLARA"
     gene            complement(3066..4484)
                     /locus_tag="VFU45_01710"
     CDS             complement(3066..4484)
                     /locus_tag="VFU45_01710"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEU5357806.1"
                     /translation="MRARGTPYLLQVLGLALTYIVGARLGQFTAIAPGNVSALWAPSG
                     IAVAAIALFGTRVWPGIWLGSFAYNFGFFHGGIAIDPSAAMGVAIGIATGSTLMAVAG
                     RYAVQLIQGPVPTVDSPRDALLWTLVAGFLAALISATVGTTMLSLGGILPLAAPVVWR
                     TWWLGDATGVVLIAPVVILWAQHGVLRFRAGQETETLAASAAFGVICFLVFGTTAPRT
                     SAPYLLAVPVLFLSYTALRFGRRITATTALVLAVVAGVATASGRGPLHATEYPEALVE
                     LQAFLAFGATLSLTLAGAVVQSRPAASEPAPALPAETGAFPIAELVSFRRTAPAVPAS
                     PAAPDPATSIAAPPPTAPAAPTTDSDLILGALPEPVYVLTRGTLAVSRCNLAFAQALG
                     FADRAAVEGRSLFDLFPPAVALAAADESRRVFHTGEARETRERWAGTDPASPEFEVSR
                     TPLRDAEGGVEALLARAHRVLG"
     gene            complement(4536..7295)
                     /locus_tag="VFU45_01715"
     CDS             complement(4536..7295)
                     /locus_tag="VFU45_01715"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5357807.1"
                     /translation="MKRLTGQVLALLAAATPLAAQGTGVISGRVHDVVSGRGLHGAMV
                     RLVGGPAVQTDTDGTFRIREVHPGYWTITVAQIGYNPVTRDSLVVRPGEVLRLEVALS
                     SVAIRVADIGITVAQDRLLDPLAPAETQRITAADLHNLPVSSLDDALAVSAGAVGQSY
                     RGGRVGEQAFILDGVGFKNQLDASTGGLGVRLPPDLLTEASLVTNGFSARYGQAISGL
                     VNVVTRDGGDHWEGRTAYETDRPFGKGWDYGLDRAVVTADGPLPGGVRILGVLDVTGR
                     VDADPVNAPPPTDPRDPRSQSPVLPGNSGEQYDGAIKLSVPVLGAPLRMLLVRSVEQR
                     RLLDPAFRYDISGSPAERVTGTLVSAQVQRSLGSGVNPLLLDLRFGYFDRDFLRGTPT
                     GPADYAFGGFTGRPLHVIGEDLARRQDTIAAEGPIPGIVGPQLTDQSPYGVPAFFLGG
                     GSRGDLAWNHYRETRVQADFTAGAGENVDYYFGGEASFQRVQTFQRALGYLPVGDSVP
                     KPSAADFSPIAVAAYTEMQARMQDLALTAGLRYDLFDARTSDATQSRGAHGSLNPRLA
                     VSTVLKGATFVASWGRFSQAPDYQYLVDAAFDDTTRTGRFRVGNPDLGFEQATQYEFS
                     VRSRPSDKVSLKVGVYVKRLDKLVASVPFGLNPDSTFFGNLDHGSVRGLEVIAEREVI
                     NGLRLRASYTLQEATASGSDAFRLRTLYLDPVTGDTVRPASDEFPLDYDRRHNFTGIV
                     EYRAPDRAGPSVLGGSVFGGLSAAAILRYQSGLPYSRYSATGDTLVGPPNSYRLPDQT
                     NVDLLVRKPFRLLGRDGSVYFDARNLFNSRDVIAVDRVTGTPGVSDATVTAMATAAYD
                     AHPEAIPYESPRYRSWADLNHDGLISGPAELMPLYLEAARDYTRPLFTYGPPRLVRVG
                     MELRF"
     gene            complement(7292..8317)
                     /locus_tag="VFU45_01720"
     CDS             complement(7292..8317)
                     /locus_tag="VFU45_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357808.1"
                     /translation="MAPTRDCGTWLAVALIATLGCAHVDPGAPSDPPIARPHDPTPPV
                     RLTLNPGQDHWPAFTVDGRGIWYAYEDLDRTDHDQCLGQLPAGGGTRTVSACQDAPVA
                     AAESLDVASSPAVHDGQVAWMRVTSPIGGVLPDGGDLVVASATDLSAPRSVRHFPFVA
                     PSGRYQSFGTDVQWLDDSTLAYVGVQINYNSISHDTSIGGVEVTLVHLAGTSPATSVV
                     GGTLGATSLSAGPTPGELFFTRGDTRIYQMQLPDTVPTVAFDFGALGSAEDVRVAGSR
                     MLAVVGDSAGRGGSIYLVDGGVAAAVTPPGTYWHHPALRSDARAFAAERFDTLTATYD
                     IYSDSLP"
     gene            8515..11205
                     /locus_tag="VFU45_01725"
     CDS             8515..11205
                     /locus_tag="VFU45_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5357809.1"
                     /translation="MLILRAAAFAWSVLLLRRLRDWRIGLLSALLLLLTLDHLFRPAA
                     LPVPGLLLSVVALALVGFLARSFEDHRRAADALRIEKAYFEHLFENAPEPMVLADAHG
                     AIQRVNSEFTRLFGYTADEVRGQSLDEILAPHALTEARLLTSDSAGGRTVRFDTQRRR
                     RDGALVHVSIIGSPIVTDGRQLGVYAVYRDNTQRVRAEEALKTSDRYFRSLMANTLDI
                     IIVLDTDGKLRYASGSIEAVLGYEAEELLGRDAFGVVHPDEVGHVRALFLRQLDRPGP
                     APRMEFRCRHRDGSWRIFEASANNLLADPAIDGVVVTMRDITQRKRSEEALRESEERY
                     ALAERGANDGLWDWNLRTGRVYFSPRWKAMIGAAEHEVGTAPEEWFGRVHPDELEQLK
                     MDIDLHVTGHTPHLETEHRMRHRDGLYRWVLTRGLAVRDSTGRAYRLAGSQTDITARK
                     SVEEQLLHDAFHDPLTGLPNRALFMDRLGHAIRRGRRSDSRSAVLFLDLDRFKVVNDS
                     LGHVRGDQLLVAIARRLEDCVRPGDTVARLGGDEFVVLLDEVPTVEEAEEIAGRIQES
                     LDVPFQLAGQEVYTTASIGIAISSTGHERAEDLLRDADTAMYDAKAQGKATHRVFHQG
                     MHSHAMTQLQLETDLRRALDRGELVLFYQPIVSLAHGRVTGFEALVRWRHPERGFLGP
                     EEFIPLAEETGLIQRIGQWVLGEACRTLRGWLDQHPGYGPLSVSVNISARQFQTPELV
                     AEVAAVLTDVPLPPGALRLEITEGTIMGQAESSVAVLRGLKQAGVQVQVDDFGTGYSS
                     LSYLQRFQLDALKIDRSFVGALGNPGENPEIVRTIITLAKNLGMAVIAEGVETPRQRT
                     ILQQLGCDYVQGWLFSHPLDVEAAAALLGVELAGGTRRPG"
     gene            11268..11870
                     /locus_tag="VFU45_01730"
     CDS             11268..11870
                     /locus_tag="VFU45_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357810.1"
                     /translation="MPRATAPARDPVPLEERARADLSFIRRTMAGAAEFTDVPGRGLV
                     AVGITAIGAAAIAAGASDPRRWLSTWLLEAALAAILGTTATVHKIRAHEGPGFTPAAR
                     KFLLSFVPAVVAGAALTAAVGADRALLPGLWLLLYGSSVVTAGAYSVRVVPVMGLAFM
                     ALGGAALLPAAAGRVDWLLAAGFGGLHVGFGWVIARRHGG"
BASE COUNT         1689 a         4055 c         4511 g         1691 t
ORIGIN      
        1 gtagcgcgcc gggtcgatgt cggcgccgcc ggaaatgatc agcccctcga gggccgcggc
       61 caccgcgccg gcggaagaag ccggcgtggt cggcgggagc acgagcgcga tgccgccggc
      121 ggcggcgacg gcggcgacgt acgccgcgtt gacgcccgcg tggaattcgc cgccgctgcg
      181 gcgcacacgt ccggtgatcc cgatccgcgg gctcatcgga ccgtggaatc cgggaagatg
      241 gggcagtggg gatcctcgac cttggccggc aagagcgaga tgccgatcag cggcaccgtg
      301 gagtcggcga gcaccacgga gccgatgacc ggggcgccgc ggacgatctc tccctcatat
      361 accaccacgc cgagggcccg gtaggccgtg tcctggacca ggaccccgta cccggcccgg
      421 aacttcggtg gcggcgctcc gatgaccccg atgacccagc ggtcgtggtc gcgccccacc
      481 agcgcaggga gggccggccc ggccacgagc cggacgggga gcttcccggg gagggatccc
      541 gggggcgtgg cgagggtctg gcccggggtc gagaactcgc cgaggatggc cagcggcccg
      601 aacatcgccg ccagaccgca ggtcacgctg ccgccggccg ggcggccgca cccggcgagt
      661 cccaccagca ggagtagcgt gcggcgcata gggctcccgg gaaggcgtca ccggcctaaa
      721 ggtaaccgct cgccgaggtc acgggcgggc cgcgcttgac gagccgcccg cgggggaatg
      781 acatttgagc ggctcacctc cactcccatt tcccgggcgc atccgtggga ttcttcgcgc
      841 gtctgcgcga ccgcctcgac cggctgatcg acggggccac cgtgccgccc accccgcggg
      901 agtcggctgc ggcgctgcag caggcgctca tcgacctcaa ggtcggcctc ggcgacctcc
      961 gccgggcgcg ggaatccacc gaacgggagc tggccgagga gcgtcgtcag ctcgccgatg
     1021 cggagcgccg gggcgcgctt gcggcggcca tccctgatcc ggagacggtg cgggtggccc
     1081 aggagttcgc cggccggcat cgggagcgcg tggcggtcct cgagcggaag cttgccgtgc
     1141 agatggatga ggtggcgctg gcggagcggg aggccgcgga gctggtggag cggtggcggg
     1201 aggcacggcg ggggctcggt ccgtccggga tgacgccgag cgtcgaagag gcgtggcgcg
     1261 gcctcgaagc ggccggggcc gaccgtcccg aactcgacct cgagcatgac ctgctccggc
     1321 accaggccga ccgcgcggcg cgggaccagg ccgccgacct tcaattgcag gcgctcaaga
     1381 agaagcttgg caaggaatag ttggtgagcg accagcggtc gctcctcccg gatcatcctc
     1441 gcaccctcac cgaccaccag gagcatcgcg tggcgtcgaa ctcgtccgac accgccttct
     1501 tcggccatcc ccggggactg gcgaccctct tcgggaccga gatgtgggag cgcttctcct
     1561 tctacggcct tcgcgcggtc gtcatcctgt acatgaccgc tgccctcgag aacggggggc
     1621 tcggctggag cgtggggaag gccggggcca tctacgggat gtacgccgcg ctcgtataca
     1681 tgctctcgct tccgggcggc tgggtggccg acaagctgat cgggctgcgc cgagccgtgc
     1741 tctatggcgg catcctgatc atgctgggcc agctcacgct cgcgatcccc ggcgccgcat
     1801 tcctgttccc aggcctcggt ctcatcatcc tcggcaccgg cctgctcaag ccgaacgtga
     1861 gcgtgatcgt cggcgagctc tatccggaga aggacctccg gcgggacgcg ggcttctcgg
     1921 tcttctacat ggggatcaac ctcggtgcca ccatcgcccc gctcatcgtg ggggggctgg
     1981 cgcaggcacc gggattccgg tcgctcctta cgtcgtgggg attcaatccc gtcaacgcgt
     2041 ggcactgggg attcggggtc ggcgcgctcg ggatgctggc gggcgtgatc cagttccggc
     2101 tggggtggaa gcacctcggg acggcgggcg cggcgccaag cgtggcgcgc gattcggccg
     2161 aggggcgctc ggcgtggcgg atcgcccggc tcagcgtcgt ggcggcggtg gcggtggtgg
     2221 tcgggctcgt cttcctcgtg cggggcggga cgtggacggc cgaaggcgtc gcggccaagt
     2281 tcccgtacct cttcggggtc ctcgtcgtgg cgttcttcgt gtggctcttc gggatgggca
     2341 actggacacg ggacgagcgg aagcgcctgg tggtggtgct ggtgctcttc gtcgcgtccg
     2401 cgatcttctg ggccgcgttc gagcaggccc cctcgacgct caacctcttt gccctccaga
     2461 acacccggaa cagcatcttc ggctggacct tcccctccac gtggctccag tcggtcaatt
     2521 ccctctgcat catcgcgctc gcaccggtct tcgcctggat ctggatccgg ctcggcacgc
     2581 gggacccgtc gagcccgacc aagttcgggt tcgggctcct ctttgccggt ctcggcttcc
     2641 tggtgatgat gttcgcggcc cgggcctccg ccgggggcgt gctcgtgagc ccgatgtggc
     2701 tggtggtgac gtatctcctc cacaccgtgg gcgagctctg cctgagcccg gtcggcctga
     2761 gctccatgac caagctcgcg cccgatcgcg tgaaggggat gatcatgggg gtatggttcc
     2821 tcgggacgtc gctcgggaac ctcatcgccg ggaacgtgac gcggctctac ggctcactga
     2881 cggtgccgca gctcttcggg gcggtggcgg cgttctgtat cgtgccggcg atcatcatgt
     2941 tcctgctggt ggtcccgatc cggcggatgc tcgcccgggc ctgaagctcc gcacccggcc
     3001 accaacgtgt gagggggcgg cgccggtgcg ccgccccctc atggcgttcg gcgcgccggc
     3061 cgttgtcagc ccagcacccg gtgcgcgcgg gcgaggagcg cctcgacgcc gccctcggcg
     3121 tcacggaggg gggtgcggct gacctcgaac tcgggcgagg ccgggtcggt cccggcccat
     3181 cgttcccggg tctcccgggc ctcgccggtg tggaagacgc ggcgcgactc gtcggcggcg
     3241 gcgagcgcca cggccggggg aaagagatcg aagagcgagc gcccctcgac ggcggcgcga
     3301 tcggcgaatc cgagggcctg cgcgaaggcg aggttgcagc gggacacggc cagggtcccg
     3361 cgggtgagca cgtagaccgg ctcaggcagg gcgccgagga tgaggtccga gtcggtggtg
     3421 ggggcagcag gcgcggtcgg gggcggcgcc gcgatggagg tcgccgggtc cggcgctgcg
     3481 ggtgacgccg gaaccgctgg cgccgtccgg cggaacgaga ccagctcggc gatggggaag
     3541 gcgccggtct ccgccggcaa tgccggtgcc ggctcgctcg ccgccgggcg gctctggacc
     3601 acagcgccgg ccagcgtcag gctgagggtc gcgccaaatg cgaggaatgc ctggagctcg
     3661 acgagcgcct cgggatactc cgtcgcgtgc aaggggcccc ggccggaggc ggtggccacg
     3721 cccgccacca ccgcgaggac cagcgcggtg gtggcggtga tccggcggcc gaaccgaagc
     3781 gcggtgtagg aaaggaagag caccggcacc gcgaggaggt acggggcgga ggtccgcggg
     3841 gccgtggtgc cgaagaccag gaagcagatg acgccgaacg ccgcggaggc ggccagcgtc
     3901 tcggtctcct gcccggcccg gaagcggagc accccgtgct gggcccagag gatgaccacg
     3961 ggcgcgatca acacgacgcc ggtggcgtcg ccgagccacc aggtgcgcca gaccaccggc
     4021 gccgccagcg gaaggatgcc gccaagcgag agcatcgtgg tcccgaccgt cgcactgatg
     4081 agggcggcga ggaaccccgc gaccagggtc cagagcaggg cgtcgcgcgg ggagtcaacg
     4141 gtcggcaccg gcccctggat cagctgcacg gcataccgcc cggcgacggc catcagggtg
     4201 ctgccggtcg cgatcccgat cgcgacgccc atcgccgcgg acggatcgat ggcgatgccg
     4261 ccgtggaaga agccgaagtt gtacgcgaac gatccgagcc agatgcccgg ccagacccgc
     4321 gttccgaaga gggcgatggc cgcgacggcg atcccggacg gcgcccagag ggcactgacg
     4381 ttccccgggg cgatggccgt gaattggccg aggcgggcgc cgacgatgta cgtcagcgcc
     4441 aggccgagga cctgcaggag atagggggtg ccgcgggcgc gcatgagccg gcaatgtacc
     4501 gcccggggcg ggtggtggcg agcgcgccag cccggctaga agcgcaattc catccccacc
     4561 cgcacgagcc ggggcgggcc gtaggtgaag agcggccggg tatagtcccg cgcggcctcg
     4621 agatagagcg gcatcagttc ggccggtccg gagatcagtc cgtcgtggtt gaggtcggcc
     4681 caggaccggt atcgcggcga ttcgtatggg atcgcctcag gatgggcgtc gtacgccgcc
     4741 gtcgccatcg cggtgacggt cgcgtcggac acgcccgggg tcccggtgac gcggtcgacg
     4801 gcgatgacat cgcgcgaatt gaagagattg cgagcgtcga agtacacgct cccgtcacgg
     4861 ccgaggagcc ggaacggctt gcgcaccaga agatccacgt tggtctggtc ggggagtcgg
     4921 tagctgttgg gcgggccgac cagcgtgtcg ccggtggcgc tgtaccggga gtagggaagg
     4981 ccggactggt agcgcaggat cgccgccgcg ctcaggccgc cgaagaccga ccccccgagg
     5041 acgctcgggc ccgcgcggtc gggggcgcgg tattccacga tgcccgtgaa gttgtggcgc
     5101 cgatcgtagt cgagcgggaa ctcgtcgctg gccgggcgga cggtgtcgcc ggtgacgggg
     5161 tcgaggtaca gggtgcggag ccggaatgcg tcggagcccg atgccgtcgc ctcctgcagc
     5221 gtgtacgagg cgcgcagccg caggccgttg atgacctcgc gctcggcgat gacctccagg
     5281 ccgcgcacgc tcccgtggtc gagattgccg aagaaggtcg aatccgggtt gaggccgaac
     5341 gggacggagg ccaccagctt gtcgagccgc ttgacgtaga cgccgacctt gagcgacacc
     5401 ttgtcggacg ggcgcgaccg gacgctgaac tcgtactgcg tggcctgttc gaagcccagg
     5461 tcggggttgc cgacccggaa gcggccggtg cgggtcgtgt cgtcgaaggc cgcatcgacc
     5521 aggtactggt agtcgggcgc ctggctgaac cgaccccagc tcgcgacgaa cgtcgccccc
     5581 ttcagcaccg tcgagaccgc caggcgcggg ttgagcgagc cgtgggcgcc gcgggactgc
     5641 gtcgcgtccg acgtgcgggc atcgaagagg tcgtagcgga gcccggctgt gagcgcgagg
     5701 tcctgcatcc gtgcctgcat ctcggtgtac gccgccacgg cgatgggcga gaagtcggcg
     5761 gcgctgggct tgggcacgct gtcgccgacc gggaggtagc cgagcgcccg ctggaacgtc
     5821 tgcacccgct ggaacgacgc ctcgccgccg aagtagtagt cgacgttctc gccggcgccg
     5881 gcggtgaagt cggcctgtac ccgggtctcg cgataatgat tccacgcgag gtcgccgcgc
     5941 gacccgccgc ccaggaagaa ggcaggtacg ccgtacgggc tctggtcggt gagctggggc
     6001 ccgacgatgc cgggaatggg gccctcggcg gcgatcgtgt cctggcgtcg cgcgagatcc
     6061 tcgccgatca catgcagtgg ccggccggtg aaaccgccga aggcgtagtc ggccgggccg
     6121 gtgggggtgc cccggaggaa gtcgcggtcg aaatagccga accggaggtc gagcaggagc
     6181 ggattgacgc ccgatcccag gctccgctgg acctgggcgc tgacgagcgt ccccgtcacg
     6241 cgctctgccg gcgagccgct gatgtcgtac ctgaacgcag ggtccagcag ccgccgctgc
     6301 tcgaccgacc gcaccagcag catccggagc ggcgcgccca ggaccgggac cgacagcttg
     6361 atggccccgt cgtactgctc gccgctgttc cccggcagga cggggctctg ggagcggggg
     6421 tcgcgcggat cggtgggtgg gggggcgttg acgggatcgg catcgacccg accggtcacg
     6481 tcgagcacgc cgaggatgcg gaccccgccg gggagcggtc catcggcggt gaccacggcg
     6541 cggtcgaggc cgtaatccca ccccttgccg aaggggcggt cggtctcgta ggccgtgcgg
     6601 ccctcccagt gatcgccgcc gtcccgggtg acgacgttca cgagcccgga gatggcctgg
     6661 ccgtagcggg cggagaatcc gttggtgacg agcgacgcct cggtgaggag gtcgggcggg
     6721 aggcggacgc cgagtccgcc ggtcgaggcg tcgagctggt tcttgaagcc gacgccgtcg
     6781 aggatgaacg cctgctcacc cacgcgtccc ccccggtagc tctggccgac ggcgccggcg
     6841 gagaccgcga gagcatcgtc gagggaactg accggcaggt tgtgcaggtc tgcggcggtg
     6901 atccgctgcg tctcggcggg tgccagcggg tcgaggagcc tgtcctgggc cacggtgatg
     6961 ccgatgtcgg cgacccgtat ggcgaccgat gacagcgcca cctcgagccg gagcacctcg
     7021 ccggggcgaa cgacgaggct gtcccgggtg acggggttgt agccgatctg ggctacggtg
     7081 atggtccagt atccggggtg gacctcccgg atccggaacg tgccgtcggt gtcggtctgg
     7141 accgccgggc ccccgacgag gcggaccatc gccccgtgca gtccacggcc cgaaacgacg
     7201 tcgtgcacgc ggccggagat gacgccggtg ccctgcgcgg cgaggggcgt cgccgcggcg
     7261 agcagggcca gtacctgtcc cgtgagccgc ttcacggcag ggaatccgag tagatgtcgt
     7321 atgtcgcggt gagggtatcg aagcgttcgg cagcgaaggc ccgtgcgtcg gaccggaggg
     7381 ccggatggtg ccagtacgtg ccgggcggcg tgacggctgc ggccaccccc ccgtccacga
     7441 ggtaaatcga gccgccgcgg ccggccgaat cgccgaccac cgcgagcatc ctgctgccgg
     7501 ccacccggac atcctccgcg ctgccgaggg cgccgaagtc gaacgcgacg gtcgggacgg
     7561 tgtcgggaag ctgcatctgg tagatgcggg tgtcgccgcg ggtgaagaac agctctcccg
     7621 gcgtggggcc ggctgagagg ctcgtcgcgc cgagggtgcc gccgaccacg gacgtggcgg
     7681 ggctggtgcc ggcgaggtgc acgagcgtga cctcgacccc gccgatggac gtgtcgtgcg
     7741 agatggagtt gtagttgatc tggaccccga cgtaagcgag cgtcgagtca tcgagccact
     7801 gcacgtcggt gccgaacgac tgataccggc cgctcggcgc gacgaacggg aagtgccgta
     7861 ccgatcgtgg ggcgctgagg tccgtggcgc tggccacgac gaggtcgcca ccatccggca
     7921 gcacgcctcc gatcggagag gtgaccctca tccacgcgac ctggccgtcg tggaccgccg
     7981 ggcttgaagc aacgtcgagc gattccgccg cggcgaccgg tgcatcctgg caggcggaga
     8041 cggtgcgggt gccaccgccc gcgggcagct ggccgaggca ctggtcgtgg tcggtgcggt
     8101 cgaggtcctc gtaggcgtac cagatcccgc ggccgtcgac ggtgaacgcc ggccagtggt
     8161 cttgtccggg attgagcgtc aagcggaccg gtggggtcgg atcgtgcggg cgggcgatgg
     8221 gcgggtcgct cggcgcgccg ggatcgacgt gggcgcagcc gagggtggcg atgagcgcca
     8281 cggcgagcca ggtgccgcag tcacgcgtcg gtgccatgtg gtccggggcc agggggatgc
     8341 ggcggcggat cggatcgccg caacttatca ggacgaacga tcggccgcct gatggacacc
     8401 gggcgccgcg ttcccaacgg gacgatccgg gggtagctta caggtccccg agcacaaccg
     8461 gtcactcccg ccgtccgagg ccgttcgccc cacgtgacgc ctgtcgacct tgtcgtgctc
     8521 attctccgtg ccgccgcctt tgcctggtcg gtgctgctcc tccgccgtct gcgcgactgg
     8581 cggatcgggc tgctgtcggc cctgctcctg ctcctgaccc tcgaccatct cttccgtccg
     8641 gcggcgctgc cggtgccggg tctcctgctc agcgtcgtgg cgctggcgct cgtcggtttc
     8701 ctggcccgtt cgttcgagga ccaccgccgc gccgccgacg cgctccgcat cgagaaggcc
     8761 tacttcgagc acctgttcga gaacgccccg gagccgatgg tgctggccga cgcccacggc
     8821 gcgatccagc gggtgaacag cgaattcacc cggctcttcg gctacaccgc cgacgaggtg
     8881 cggggccagt cgctcgacga gatcctggcg ccgcacgcgc tcaccgaggc ccggctgctg
     8941 acctcggatt cggcgggcgg gcggacggtc cgcttcgaca cccagcgccg ccgccgcgac
     9001 ggcgcgctgg tgcatgtctc gatcatcggc agcccgatcg tcaccgacgg ccgccagctc
     9061 ggcgtctacg ccgtgtaccg ggacaacacc cagcgggtgc gggccgagga ggcgctcaag
     9121 accagcgacc gctacttccg ctcgctgatg gccaacacgc tcgacatcat catcgtgctc
     9181 gacaccgacg ggaagctccg ctacgccagc gggtcgatcg aggcggtgct cgggtacgag
     9241 gccgaggagc tgctggggcg cgacgccttc ggggtggtcc atccggatga ggtgggccac
     9301 gtgcgggcac tgttcctccg ccagctcgac cgccccgggc ctgcgccgcg gatggagttc
     9361 cgctgccggc accgcgacgg gtcgtggcgg atcttcgaag cgagcgccaa caacctcctg
     9421 gccgaccccg cgatcgacgg cgtggtggtc accatgcgcg acatcaccca gcgcaagcgc
     9481 tccgaggagg cgctccgcga gagcgaggag cgctatgcgc tggccgagcg cggcgccaac
     9541 gacgggctct gggactggaa cctccgcacc ggccgggtgt acttctcgcc ccgctggaag
     9601 gcgatgatcg gcgccgccga gcatgaggtg ggcaccgcgc cggaggagtg gttcgggcgg
     9661 gtccaccccg acgagctcga gcagctcaag atggacatcg acctgcacgt gaccgggcac
     9721 accccgcacc tcgagaccga gcaccggatg cggcatcgcg acgggctcta ccgctgggtg
     9781 ctgacgcgtg gcctggcggt gcgcgacagc accggccgcg cgtaccggct tgcggggtcg
     9841 cagaccgaca tcacggcgcg gaagtcggtc gaggagcagc tcctccacga tgccttccac
     9901 gacccgctca ccggcctccc gaaccgggcg ctcttcatgg accggctggg gcacgccatc
     9961 cggcgcggcc ggcggagcga cagccgctcc gcggtgctct tcctcgacct cgaccggttc
    10021 aaggtggtca acgacagcct gggccacgtc cggggcgacc agctgctggt cgccatcgcc
    10081 cggcggctgg aggactgcgt ccgccccggc gacacggtgg cgcgcctggg cggcgacgag
    10141 ttcgtcgtcc tgctcgacga ggtgcccacc gtcgaggagg cggaggagat cgcgggccgg
    10201 atccaggaat cgctcgacgt cccgttccag ctggcggggc aggaggtcta caccacggcg
    10261 agcatcggga tcgccatcag ctccacgggg cacgaacggg cggaggacct cctccgcgac
    10321 gccgacacgg cgatgtatga cgccaaggcg caggggaagg ccacgcaccg cgtgttccac
    10381 caggggatgc acagccacgc gatgacccag ctccagctgg agaccgacct gcggcgtgcg
    10441 ctcgaccgcg gcgagctggt gctcttctac cagccgatcg tgtcgctggc ccacgggcgg
    10501 gtgaccggct tcgaggcgct ggtccggtgg cggcaccccg agcgcggctt cctcgggccc
    10561 gaggaattca tcccgctggc ggaagagacc gggctcatcc agcgcatcgg ccagtgggtc
    10621 ctgggcgagg catgccgcac ccttcgcggc tggctcgacc agcatccggg ctacgggccg
    10681 ctctcggtga gcgtcaacat ctcggcgcgc cagttccaga cgccggagct ggtggcggag
    10741 gtcgccgcgg tgctcaccga cgtgccgctg ccccccgggg cgctgcggct ggagatcacc
    10801 gaggggacga tcatgggcca ggcggagtcg agcgtggcgg tgctccgggg gctcaagcag
    10861 gccggggtgc aggtgcaggt ggacgatttc ggcaccgggt actcctcact cagctacctg
    10921 cagcgcttcc agctcgacgc actcaagatc gaccggtcgt tcgtcggggc gctggggaac
    10981 ccgggggaga acccggagat cgtccggacg atcatcacgc tggccaagaa cctcgggatg
    11041 gccgtcatcg ccgagggggt ggagacgccc cgccagcgca ccatcctcca gcagctgggg
    11101 tgcgactacg tgcaggggtg gctcttctcc cacccgctgg acgtcgaggc cgccgccgcg
    11161 ctgctcggcg tcgaactcgc cggcggcacc cgccgtccag ggtaagtggt gtagcttcac
    11221 ttcgcaaagt acttgacgtt ctggccgccg ctcgctacca tctccccatg ccccgagcca
    11281 ccgcacccgc ccgcgacccc gtgcccctcg aggagcgcgc ccgcgccgac ctgtcgttca
    11341 tccgccgcac gatggcgggg gcggcggagt tcaccgatgt cccgggccgg gggctggtgg
    11401 cggtgggcat cacggcgatc ggggccgcag ccatcgctgc cggcgcgtcc gacccgcgcc
    11461 gttggctttc cacctggctc ctcgaggccg ccctggccgc gatcctcggc accacggcca
    11521 ccgtgcacaa gatccgcgcg cacgagggtc cggggttcac gccggccgcg cgcaagttcc
    11581 tcctgagctt cgtgccggcg gtcgtggccg gcgcggcgct cacggcggcc gttggcgccg
    11641 accgggcgct cctgcccggg ttgtggctcc tcctgtacgg cagcagcgtg gtgacggcgg
    11701 gcgcctattc ggtgcgcgtg gtgccggtga tggggctggc gttcatggcg ctgggcgggg
    11761 cggcgctcct cccggccgcg gcggggcggg tcgactggct ccttgccgcc ggcttcgggg
    11821 gcctccacgt cggattcgga tgggtcatcg cgcggaggca cggtggctaa gggtggcgcg
    11881 atgcccaagc gcgcagccgg caccgaccag gcactcggcg cggtgacggg cgacccccgg
    11941 gcgcca
//
LOCUS       DASUAP010000069         4510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1146349, whole genome shotgun sequence.
ACCESSION   DASUAP010000069 DASUAP010000000
VERSION     DASUAP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146349"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFU45_01735"
     CDS             <1..500
                     /locus_tag="VFU45_01735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEU5357811.1"
                     /translation="LAYRPGFRGQVVAADFVPEMLALGRHKAVSVRPLTADALELPFA
                     DATFDGVTIGFGVRNFSDIDAGLREVARVLKPGARLVVLDCSQPPFKPFRALYYFYFR
                     HLLPRIGRLISRHADAYTYLPESVLAFPGAEMLRAHMEGAGFERVEYRRLLLGVVAIH
                     AGVRR"
     gene            500..2263
                     /locus_tag="VFU45_01740"
     CDS             500..2263
                     /locus_tag="VFU45_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEU5357812.1"
                     /translation="MHRPWPDLGSYVAELERLGQLKRVNVEVDPKFEVAEIIQRVIRE
                     GGPALLFERVKGARFPLVMNLFGSDRRIELALGRHPQEIGQELVALIQRLNPPSLKAF
                     WAARGQLLRARHLRPRSVRSGPVQEVVEAPALTSLPNLWSWPRDGGPFITWGPTLTVS
                     PANGRRNFGLYRLQVFDDRTTGMHWQSMKGGRGHHYEAERLGRPLEAAVVLGGDPVTM
                     LSAIFPLPEDFDELGFAGYVRGAATRLVRGRSISLDVPANAEVVLEGVVPPGERRMEG
                     PFGDHYGHYSEAELFPVFHVRTMTRRRAPIVPAAVVGIPPQEDKWIGLAVGDIVGPLI
                     KVINPNIVDLFASDDAVFHNLLVVSCKERHPKEVLKTAFNLLGTGQLALTKVTVMVRE
                     DVPVRDLRRVLRELWHRFDPGERMLLIPTAPLDTLDYTSFTLHVGSKLVLDATGDVVQ
                     PAEPPRAVEDPAGFDPRVTGHRLVEGFLVVRVRERPRDVLQRLVQWEGLGPVRFIAAV
                     SEDVDLDDRTSLIWGIFTRFDPARDMLFQEQRFVGAKPVYGGRIGIDATWKPGYPPVV
                     QMPEETVRSVDRRWGEYFGER"
     gene            2443..3381
                     /locus_tag="VFU45_01745"
     CDS             2443..3381
                     /locus_tag="VFU45_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEU5357813.1"
                     /translation="MTHDSRPTTHDPRPATRREGQTFEGRSLLVRYVNFVKLPHTLFA
                     LPFALLGVLAASFQVPVTWRTLGLVVVAFSAARWVAMGFNRIADRAIDARNPRTLMRE
                     LPQGALTLGQAWASVAAAGALFVLAAWLLNPICFALSPVALAWVMVYSLSKRFTSLAH
                     LWLGLGMAIAPVGGYLAITGRWSTPWWLLVAVAVAVMTWGAGFDIFYALPDEGFDRDE
                     GLRSLVVRLGQGRAILAAKLLHGVTIPALVLFGYAAGFGLWYYSGLVVAAAIRASEHQ
                     LVRPGDLSRLDAAFFTMNGVMSVTVFAFALLDRLAR"
     gene            3452..3787
                     /locus_tag="VFU45_01750"
     CDS             3452..3787
                     /locus_tag="VFU45_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357814.1"
                     /translation="MAGKQKPAKPEAKGKSGAKGKNEVKGKQVSKAAPAAPVVAAAPA
                     APAPASAAPAPARPVEKKAKPVPPRHGIPLPAHGSKFFGAPKQGKRPGPRVPPAHPVH
                     MPTGNRRGG"
     gene            3784..4368
                     /locus_tag="VFU45_01755"
     CDS             3784..4368
                     /locus_tag="VFU45_01755"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEU5357815.1"
                     /translation="MSERPLVVAVTGASGAPYAVRLVEVLARHRVPAWLLVSSHGQRL
                     LQEECGIRNVDELKAATGGDWSSVTHFPDGDRGARPASGSVPTRGMVICPCSMGTAGS
                     IAAGTSRSLIERAADVTLKERRPLILVPRETPLSLIHLRNLTALTEAGATILPAAPGF
                     YHRPREIAELVDFVVQRILDQVGLEIEVTRRWGS"
BASE COUNT          628 a         1581 c         1611 g          690 t
ORIGIN      
        1 agctcgcgta tcgtccgggg ttccggggac aggtcgtggc ggcggacttc gtgccggaga
       61 tgctggcgct ggggcggcac aaggcggtga gcgtccgtcc ccttaccgcc gacgcgctgg
      121 agcttccctt tgccgacgcg accttcgacg gcgtcacgat cgggttcggg gtccggaact
      181 tcagcgacat cgacgccggc ctccgcgagg tggcgcgggt gctcaagccc ggcgcccggc
      241 tggtggtgct cgactgctcc cagccgccgt tcaagccgtt ccgggcgctc tactacttct
      301 acttccggca cctgctcccg cgcatcggcc ggctgatctc ccggcacgcg gacgcgtaca
      361 cgtaccttcc cgagtcggtc ctggccttcc ccggggcgga aatgctgcgg gcgcacatgg
      421 agggtgcagg attcgagcgt gtggagtacc ggcggttgct gctgggggtc gtggcgatcc
      481 acgcgggcgt gcggcgctga tgcaccgtcc gtggccggac ctgggatcat acgttgccga
      541 gctggagcga ctcggccagc tcaagcgcgt caacgtcgag gtcgatccca agttcgaggt
      601 cgcggagatc atccagcggg tcatccgcga gggcggtccc gcgctcctct tcgagcgggt
      661 caagggcgcg cgcttcccgc tggtgatgaa cctcttcggc tccgaccgcc ggatcgagct
      721 ggcgctcggg cgccacccgc aggagatcgg ccaggagctc gtcgcgctca tccagcggct
      781 caatccgccg tcgctcaagg cgttctgggc cgcgcggggc cagctgctgc gcgcccggca
      841 cttgcggccc cggtcggtga ggtccggccc ggtgcaggag gtggtggagg cgccggcgct
      901 cacctcgctg cccaatctgt ggagctggcc gagggacggc ggacccttca tcacctgggg
      961 accgacgctc accgtctcgc ccgcgaacgg ccggcggaac ttcggactct atcgcctgca
     1021 ggtcttcgac gaccgcacca ccgggatgca ctggcagtcg atgaaggggg ggcgcgggca
     1081 ccactacgag gcggagcgcc tggggcgccc gctcgaggcg gcggtggtgc tggggggcga
     1141 cccggtcacg atgctctccg ccatcttccc gctccccgag gatttcgacg agctggggtt
     1201 tgcgggttac gtgcgaggtg cggccacccg gctcgtgcgc ggacggtcga tctcgctcga
     1261 cgtaccggcc aacgccgagg tcgtcctcga gggggtggtg ccgcccggcg agcgccggat
     1321 ggaagggccc ttcggcgacc actacggcca ctactccgag gcggagctgt tccccgtctt
     1381 ccacgtgcgc acgatgaccc gccgccgcgc gccgatcgtg cccgcagcgg tggtcgggat
     1441 cccgccgcag gaggacaagt ggatcgggct cgcggtgggc gacatcgtcg gtccgctcat
     1501 caaggtcatc aacccgaaca tcgtggacct cttcgcgtcg gacgacgcgg tgttccacaa
     1561 cctgctggtc gtctcctgca aggagcgtca cccgaaggag gtcctcaaga ccgccttcaa
     1621 cctcctcggc accggccagc ttgcgctcac caaggtgacg gtgatggtcc gggaggacgt
     1681 gccggtgcgc gacctccgcc gggtgctgcg cgagctgtgg caccgcttcg acccggggga
     1741 gcggatgctc ctcatcccga ccgccccgct cgacacgctc gactacacgt cgttcacgct
     1801 ccacgtcggg agcaagctcg tgctcgacgc caccggcgac gtggtgcagc cggcggagcc
     1861 cccccgcgcc gtcgaggatc cggcaggctt cgatccgcgg gtgacgggcc accggctcgt
     1921 ggaggggttc ctcgtggtcc gggtgcggga gcggccgcgc gatgtgctgc agcgcctggt
     1981 ccagtgggag gggctcgggc cggtccgatt catcgccgcg gtgagcgagg acgtggacct
     2041 cgacgaccgc acgagcctca tctggggcat cttcacccgc ttcgacccgg cgcgcgacat
     2101 gctcttccag gagcagcgct tcgtcggcgc caagccggtc tacggcggcc ggatcggcat
     2161 cgacgcgacc tggaagcccg ggtacccgcc ggtggtgcag atgcccgaag agacggtgcg
     2221 gtcggtggac cggcggtggg gggagtactt cggtgagcgg tgagaggtga gagggaacgg
     2281 cgtgaacggt gaacggggaa cagtgaacgg ggaacggtga acggggaacg gtactgggtg
     2341 aacggggcat cggcgagcac gaccaacgac caacgaccaa cgactaccga ccaacgaccc
     2401 gcgacccacg acccgcgacc cacgactcac gacccacgac tcatgaccca cgactcacga
     2461 cccacgacgc atgacccgcg acccgcgacc cgccgagaag gccagacctt cgaggggcgc
     2521 tcactcctcg tgcggtacgt caacttcgtg aagctcccgc acacgctctt cgcgctgcca
     2581 ttcgcgttgc tgggcgtgct ggcggcgtcc ttccaagtgc cggtcacctg gcggaccctc
     2641 ggcctggtgg tggtggcgtt ctccgccgcg cgctgggtgg cgatgggatt caaccggatc
     2701 gccgaccggg ccatcgatgc ccggaacccc cggaccctga tgcgggaact gccgcagggc
     2761 gccctcaccc tgggccaggc gtgggcgtcg gtggcggcgg ccggggcgct cttcgtgctg
     2821 gcagcgtggc tcctcaaccc gatctgcttt gccctctcgc cggtggccct ggcttgggta
     2881 atggtgtaca gcctcagcaa gcgcttcacc agcctggcgc acctctggct cgggctcggc
     2941 atggccatcg ccccggtggg gggatacctc gcgatcaccg gccggtggag cacgccctgg
     3001 tggcttctcg tagccgtggc cgtggcggtg atgacctggg gtgccgggtt cgacatcttc
     3061 tatgccctcc ccgacgaggg cttcgaccgg gacgagggac tgcggagcct ggtggtgcgc
     3121 ctcggccagg gccgcgccat cctcgccgcc aagctcctcc acggcgtcac catccccgcg
     3181 ctcgtcctct tcgggtacgc ggccggattc gggctctggt actattcagg cctggtggtg
     3241 gccgcggcga tccgggcgtc cgagcaccag ctcgtgcgcc ccggcgacct ctcccggctc
     3301 gacgcggcgt tcttcacgat gaacggcgtg atgagcgtga cggtgttcgc gttcgcgctc
     3361 ttggaccggt tggcgcggtg atcgactgat ccgccgcatc gggtgggcag gaagagacag
     3421 gcgtctcccg tccttcacgg aggatgatcg tatggctggg aagcagaagc cggcaaagcc
     3481 cgaggccaag gggaagtccg gggcgaaggg caagaacgag gtcaaaggga agcaggtgag
     3541 caaggcggcc ccggctgccc ccgtggtcgc ggcggcgccg gcagcgcctg cgcccgccag
     3601 cgccgcgccc gcaccggccc ggccggtcga gaagaaggcc aagccggtgc caccacgcca
     3661 tgggatcccg ctcccggcgc acggttcgaa gttcttcggc gccccgaagc aggggaagcg
     3721 tccgggcccc cgggtgccac cggcgcaccc ggtgcacatg cccaccggca accggagggg
     3781 tggttgagcg aacggccgct cgtcgtcgcg gtcaccggcg cctccggggc gccgtatgcg
     3841 gtgcgcctgg tcgaggtgct tgcgcgccat cgcgtccccg cctggctcct ggtgtcgtcc
     3901 cacggccagc ggctcctcca ggaggagtgc ggcatccgga acgtcgacga gctcaaggcc
     3961 gccaccggtg gcgactggag ctcggtgacc catttccccg acggcgaccg gggtgcgcgg
     4021 ccggcgtcag ggtcggtccc cacgcggggg atggtcatct gcccctgctc gatgggcacc
     4081 gccgggtcga tcgccgccgg gacgagtcgc tcactcatcg agcgcgccgc cgacgtgacc
     4141 ctcaaggaac gccgccccct catcctcgtg ccgcgcgaga cgcccctctc gctcatccac
     4201 ctccgcaacc tgaccgcgct caccgaagcc ggcgccacga tccttcccgc cgcgccgggg
     4261 ttctaccacc gacccaggga gatcgccgag ctggtggact tcgtggtgca gcggatcctc
     4321 gatcaggtgg ggctggagat cgaggtgacg cgacggtggg gctcttagcg gcgtgcgatg
     4381 agggacgggt ggcgggtggg gcccgaggaa cgggttgcgt ggacctgacc gcgcaccgga
     4441 tccgcgaccc cgctacccga gccccgctcc ccacccgccg cctttcaccc gctacgcgct
     4501 actcgtaacc
//
LOCUS       DASUAP010000070         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_29031, whole genome shotgun sequence.
ACCESSION   DASUAP010000070 DASUAP010000000
VERSION     DASUAP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29031"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFU45_01760"
     CDS             <1..379
                     /locus_tag="VFU45_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU5357816.1"
                     /translation="LASGRTAVLDIEVQGARQVRKHFAEAVQVFILPPSGAALAERLR
                     GRLTEGQASYIARLKRAAQEMLAVSEYDFVIVNDVLDDAVAEISAILDGEGHRVTRQV
                     GLDQQVETLRREVAREADAADTQ"
     gene            394..606
                     /locus_tag="VFU45_01765"
     CDS             394..606
                     /locus_tag="VFU45_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357817.1"
                     /translation="MRIYTPVEVAKQAGNKYLGVLVAAKFARYANELPKERLAQRNQK
                     LTTVSLDALTEGDLSYKLVRRRRSEA"
     gene            614..1819
                     /gene="coaBC"
                     /locus_tag="VFU45_01770"
     CDS             614..1819
                     /gene="coaBC"
                     /locus_tag="VFU45_01770"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU5357818.1"
                     /translation="MTAWTGRHVVLGVSGGIASYKSCALARRLTEAGAQVDVVLTSGA
                     AEFVRPVTFEALTGRRVLTSLWEPDAALSHVHLAHRADLLVVAPATAHLLARAALGLA
                     DDLLTALLLARGERPLLLAPAMNDEMYAHPATQGNLALLRSRGAAFVGPEPGALAEGP
                     SERPGRMSEPETILAHAARLLRGRTRLSGKRVVVTAGPTREPIDPVRVVTNRSSGRMG
                     YRVAEAVWERGAEVTLISGPVELPPPPGVVVQRIETTAELESAVRRALEAADVLVMAA
                     APADYRPVAPSAEKRPRQDGALALPLEPTADILSATAAARRAGSVMVGFALETGDALV
                     KGRAKLERKALDLIVINDALEPGAGFEVDTNRVAILGRDGTSMILPLQSKRETAEQIL
                     DAVEARLDR"
     gene            1809..>2094
                     /locus_tag="VFU45_01775"
     CDS             1809..>2094
                     /locus_tag="VFU45_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357819.1"
                     /translation="MTGERERYLRQQAELGGREAVLGVGSLEPGVRSVVTGAKSLESG
                     VRSLERGPAVNPVAELHAPSSELQAPSSELPASDWRKGAPPIPGPGLTVVP"
BASE COUNT          287 a          725 c          808 g          274 t
ORIGIN      
        1 cctggcctcg gggcgcaccg cggtgctgga catcgaggtc cagggcgcgc gccaggtccg
       61 gaagcacttt gccgaggcgg tgcaggtgtt catccttccg ccgtccggcg ccgcgctggc
      121 cgagcggctc cggggacggc tgacggaagg ccaggcgtcg tacatcgcgc ggctcaagcg
      181 cgcggcgcag gagatgcttg cggtatccga atatgacttc gtgatcgtca acgacgtgct
      241 cgacgacgcg gtcgcggaga tctcggcgat cctcgacggc gaggggcacc gggtgacgcg
      301 gcaggtgggg ctggaccagc aggtcgagac attgcggcgg gaggtggccc gggaggccga
      361 cgccgctgac acccagtagg gcggaggcag gcaatgcgga tctacacgcc ggtcgaggtg
      421 gccaagcagg cggggaacaa gtatctgggc gtgctggtgg ccgccaagtt cgcgcggtac
      481 gccaacgagc tccccaagga gcgcctggcc cagcgcaacc agaagctgac cacggtgtcg
      541 ctcgacgcgc tcaccgaggg cgacctgtcg tacaagctgg tccgccgccg ccgctccgaa
      601 gcgtgaccgg cgcgtgaccg cctggaccgg ccgccatgtg gtcctcggcg tgtcgggcgg
      661 gatcgcgagc tacaagagct gcgccctcgc ccggcgcctg accgaggccg gcgcccaggt
      721 cgacgtggtg ctcacgtcgg gggcggcgga gttcgtgcgg ccggtcacct tcgaggcgct
      781 caccgggcgc cgcgtcctga cctccctctg ggagccggat gcggcgcttt cgcacgtcca
      841 cctggcgcac cgggccgacc tgctggtcgt cgcaccggcc acggcgcacc tcctggcgcg
      901 ggcggccctc gggctggccg atgacctgct cacggcgctc ctcctggcgc gcggggagcg
      961 gccgctcctc ctggccccgg cgatgaacga cgagatgtac gcccaccccg cgacccaggg
     1021 gaacctggcg cttctccgct cgcggggcgc ggcgttcgtc ggcccggagc ccggcgccct
     1081 cgccgagggg ccgtcggaac gcccggggcg gatgagcgaa ccggagacga tcctggcgca
     1141 tgcggcccgc ctcctgcgcg gccgcacccg gctctctggc aagcgggtcg tggtgacggc
     1201 cggtccgacc cgggagccga tcgacccggt gcgggtggtg accaaccgct cgagcgggcg
     1261 gatggggtat cgtgtggcgg aagcggtgtg ggagcgcggc gctgaggtga ccctcatcag
     1321 cgggccggtc gagctgccgc cgccgccggg cgtggtggtc cagcggatcg agacgacggc
     1381 cgagctcgaa tcggcggtgc gtcgcgcgct cgaggccgcc gacgtgctgg tgatggccgc
     1441 ggcacccgcg gactaccgcc cggtggcgcc aagcgcggaa aagcgcccgc gccaggacgg
     1501 cgcgctggcc ctcccgctcg agcccacggc tgacatcctg agcgccaccg cggcggcgcg
     1561 ccgggcaggg tcggtgatgg tcgggttcgc cctcgagacc ggcgacgcgc tcgtcaaggg
     1621 aagggccaag ctggaacgga aggcgctcga cctcatcgtc atcaatgatg cgctggagcc
     1681 cggtgccggg ttcgaggtcg acaccaaccg cgtggcgatc cttggccggg acggcacgtc
     1741 gatgatcctc ccgctccagt ccaagcgcga gaccgccgag cagatcctcg acgccgtgga
     1801 ggcgcgcctt gaccggtgaa cgcgagcgct acctgcggca gcaggccgag ctgggggggc
     1861 gggaggcggt gctgggcgtt ggcagcttgg agcctggcgt ccggagcgtg gtgacgggcg
     1921 cgaagagtct ggagtctgga gtcaggagct tggagagggg cccggcggtc aatccggttg
     1981 ccgagctcca cgctccaagc tccgagctcc aggctcccag ctccgagctc ccagcgtccg
     2041 actggcgcaa gggagcgccg ccgatccccg gcccggggct caccgtggtc cccc
//
LOCUS       DASUAP010000071         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_219950, whole genome shotgun sequence.
ACCESSION   DASUAP010000071 DASUAP010000000
VERSION     DASUAP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219950"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFU45_01780"
     CDS             complement(<1..306)
                     /locus_tag="VFU45_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU5357820.1"
                     /translation="MRDLVLAANWKMQVTPAEAREFAADFLTRVRPRLGRHLWFFPPS
                     VSLESTVRSFLDRADVRVGAQDVYWEPKGAFTGEVSVPMLQDSCRYVIIGHSERRQFF
                     "
     gene            complement(306..1559)
                     /locus_tag="VFU45_01785"
     CDS             complement(306..1559)
                     /locus_tag="VFU45_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357821.1"
                     /translation="MRRRASAAITLLAGAIGCGRSGPPAAPLAAVTRDSAGIHIIEDR
                     TALWTAGLGWSVDSLLLDLGTPDGEFQGFAAPVAMTRLSDGRIVVADAGAARLDFFSR
                     DGHFLYHVGSAGRAPGQFQSLWGVFTGRADTVVAYDVATSRVTTFDPNGALVDTLTLT
                     VPAGSNGYLPLGRSPTGDLTLLRSETPVPFPGRPWSVAIDTGVVVRFSRAGTPLDSIG
                     ALPVGELVGLPVPQAGGGTVTLPIDRPLGRKAGFVVRGDTVWVGTGAEPALVQYVGGQ
                     PIRMVRIPGALTVVPASLVAEFKRRRRAMTGRVGVIDSAFNASLDSIPFSDYLPAYGR
                     ILGDPAGDIWVQSAGLPDLAPGDASLTWSVLAPDGRWLGQVALPPRFTVREVGADYLL
                     GSWIPDSAGSPHVRLFRLRKPAGGS"
     gene            complement(1552..2736)
                     /locus_tag="VFU45_01790"
     CDS             complement(1552..2736)
                     /locus_tag="VFU45_01790"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU5357822.1"
                     /translation="MNKKSVKDVDVRGKRVYCRVDFNVPMEDGAITDDTRIRAALPTI
                     RYLCDKGCRVVLLSHLGRPKGGPDPKYSLRPVAAALEGLLGAPITFIPDALAPDAAQI
                     TKRLARGGVALVENTRFYPGEEKNDPALARQFALLGDLYVDDAFGSAHRAHASTEGVA
                     HLLKPAVSGFLMELELKYLGEALARPRRPFVAVLGGAKVSGKVDLIDALLPKVDAILI
                     GGAMACTFFRAMGLETGNSLVEADRVEMARALIAKAGAKLVLPTGAVIAERLDRGVST
                     RSVPRDAIPAGWAMYDIDAPTEAEFARRIAAAGTVVWNGPMGVFETPPFDHGTFAMAR
                     ALADATARGAVTVVGGGDSAAAVAEAGLADRMTHVSTGGGASLEFLEGKPLPGVVALD
                     DA"
BASE COUNT          441 a          987 c          974 g          339 t
ORIGIN      
        1 gaagaactgg cggcgttcgc tatggccgat gatgacgtag cggcaggagt cctggagcat
       61 cgggacgctg acctcgccgg taaaggcgcc cttcggctcc cagtagacgt cctgggcgcc
      121 gacccggaca tcggcccggt cgaggaagga ccgcacggtg ctctccagcg acaccgacgg
      181 cgggaagaac cagagatggc gcccgagccg cgggcggacg cgtgtcagga agtccgcggc
      241 gaactcgcgg gcctcggcag gggtgacctg catcttccag ttggcagcga gcacgaggtc
      301 gcgcatcatg accctccggc gggcttccgc aggcgaaaga gccggacgtg cgggctgccc
      361 gctgagtccg ggatccagga gccgagcagg taatccgcgc cgacctcacg caccgtgaac
      421 cgtggcggca gcgccacctg gccgagccag cgtccgtccg gcgccagcac cgaccaggtg
      481 agcgaagcat cgcccggcgc gaggtcgggc aggccggccg actgcaccca gatgtcgccc
      541 gccgggtcgc caaggatccg cccgtacgcc gggagatagt cggagaacgg gatcgaatcg
      601 agcgaagcgt tgaacgccga gtcgatgacc cccacccgcc ccgtcatcgc ccggcggcgg
      661 cgcttgaact cggcgacgag cgaagccggg accacggtga gcgcgcccgg gatccgcacc
      721 atgcggatag gctggccgcc cacgtactgc accaatgcag gctcagcgcc ggtgccgacc
      781 cacaccgtgt caccccggac cacgaagccc gccttgcggc ccaacggacg atcgatcggc
      841 aaggtgaccg tgccgccgcc cgcctgcggc accggtagcc ccaccagctc gccgacggga
      901 agtgcgccga tcgagtcgag cggcgtgccg gcgcgcgaga accggaccac gacgcccgtg
      961 tcgatcgcga cggaccaggg acgccccggg aacggcaccg gcgtctcgct gcgcagcagg
     1021 gtgaggtccc cggtcggtga ccgtccgagc gggaggtacc cgttgcttcc ggccggcacc
     1081 gtgagcgtca gcgtgtcgac cagcgcgccg ttcgggtcga aggtggtcac gcggctcgtg
     1141 gcaacgtcgt acgccaccac cgtatcggca cggccggtga agacgcccca cagcgactgg
     1201 aactggcctg gcgcgcgccc ggcgctcccg acgtgataga ggaagtgccc atcccgggag
     1261 aagaaatcga gccgcgcagc cccggcatcg gccaccacga tccgaccgtc gctcaggcgg
     1321 gtcatcgcca ccggggcggc aaagccctgg aactcgccgt ccggcgttcc cagatccagc
     1381 agcagcgaat cgaccgacca tcccagtccc gccgtccaga gcgcggtgcg atcctcgatg
     1441 atgtggatcc ccgcgctgtc ccgggtgacc gccgcgagcg gggcggccgg cggtccggat
     1501 cgaccgcagc cgatggcgcc agcgaggagc gtgatggccg ccgaggcgcg cctacgcatc
     1561 gtccagcgcc acgacgccgg gcagcggctt cccctcgagg aactcgagcg acgcgccccc
     1621 gccggtcgac acgtgggtca tccggtcggc cagccccgct tcggccacgg cggccgccga
     1681 atcgccgccg cccacgacgg tcacggcgcc ccgcgccgtg gcatccgcca gcgcccgcgc
     1741 catcgcgaag gtgccatggt cgaacggcgg cgtctcgaag acgcccatcg gcccgttcca
     1801 caccacggtc cctgccgcgg cgatccggcg ggcgaactcc gcctcggtcg gcgcgtcgat
     1861 gtcgtacatc gcccagccgg cggggatggc atcgcgaggc accgaccggg tcgacacacc
     1921 acggtcgagc cgctcggcga tgacggcgcc ggtcgggagc accagcttgg caccggcctt
     1981 ggcgatgagc gcccgcgcca tctccacgcg gtcggcctcc acgagcgagt tcccggtctc
     2041 aagccccatc gccctgaaga aggtgcaggc catcgccccg ccgatgagga tggcatccac
     2101 cttggggagg agcgcatcga tcaggtcgac cttgcccgac accttggcgc cgccgagcac
     2161 ggcgacgaac ggccgacgtg gccgggccag cgcctcgccc aggtacttga gctcgagctc
     2221 catcaggaag cccgacacgg cgggcttgag caggtgggcc accccttcgg tgctggcgtg
     2281 ggcgcgatgg gccgagccga aggcatcgtc gacgtacaga tcgcccagga gcgcgaactg
     2341 ccgggcgagc gccgggtcgt tcttctcctc gccagggtag aaccgggtgt tctccaccag
     2401 cgccaccccg ccacgcgcga ggcgcttggt gatctgggcc gcgtccggcg cgagggcgtc
     2461 ggggatgaac gtgatggggg cgccgaggag cccttcgagc gcggcggcga ccggccggag
     2521 cgagtacttg ggatcggggc cgcccttggg gcggcccagg tgcgacagga gcaccacgcg
     2581 gcatcccttg tcgcacaggt agcggatggt cgggagcgcc gcccggatgc gggtgtcgtc
     2641 ggtgatggcg ccgtcttcca tgggaacgtt gaaatcgacg cggcagtaaa cgcgcttgcc
     2701 gcgcacgtcc acatctttga ccgatttctt gttcatcggg g
//
LOCUS       DASUAP010000072         4627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_533323, whole genome shotgun sequence.
ACCESSION   DASUAP010000072 DASUAP010000000
VERSION     DASUAP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533323"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFU45_01795"
     CDS             complement(<1..754)
                     /locus_tag="VFU45_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5357823.1"
                     /translation="MRHIPDEELHAYLDQALSRSQAVEIESHLARCDRCRAARAECAL
                     LRDRTTALLAELGPGTIPLPPFDVVRRAAAERRTRRAVRRRAAWAASILLALGLGWGS
                     RRFLPAPLVPAPLAPKAVAHPLAEVPRQVAAARTPQVVVRRGADHPIRATVEYDSLSR
                     DPAAGPAGLWHSVSLAGAAAAGTPIPRVAGLPVLQIQLQEGPEASPVVAVDQQMSDGQ
                     TVRTIEGPAGPVWNLYTATTVPTDTAGVATLSL"
     gene            complement(738..1253)
                     /locus_tag="VFU45_01800"
     CDS             complement(738..1253)
                     /locus_tag="VFU45_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5357824.1"
                     /translation="MTDDLEALYQSTYTSLVRFLFRKVWDLDRAEDLAQEVFVRAMHH
                     QPEKPRAWVFAVAANLARDEARAAVRRKRHLTLLTSDPAGSPAPEPDAQDLVEHDERL
                     ASVHRALATLTERDQEVLLLWDAGLSYPEISLRTGLALGAVGTTLARARKRLVEAHDR
                     MTHERIDAAHS"
     gene            complement(1437..2144)
                     /locus_tag="VFU45_01805"
     CDS             complement(1437..2144)
                     /locus_tag="VFU45_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357825.1"
                     /translation="MTLRVGGLQDGEASLDDEATWLLGHTGNITFGRVWREEQSLFAE
                     ATLHVPCRHLREGPGEDICKAHGHRGPAPAPTTRTPAPRRLGDSEFEVVEGLALARRT
                     LPLAPRPASLPVYAGTNPCLAAPCRTADNTRGAACCRDLQLGIRCDRGDVALEALVRA
                     RRSPYLCKVDRESDRYLGVEMISSCAYLDDDARSCALHGRIRSDGRPAKPELCFDWPD
                     GEVFHAGCVFRTDALRA"
     gene            2372..2806
                     /locus_tag="VFU45_01810"
     CDS             2372..2806
                     /locus_tag="VFU45_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5357826.1"
                     /translation="MAACGGGEKKAETPAAAPAAAAAAPAAAPATNAPVHVVEMTLDG
                     TTPKYNPSELTIKQGEIVRFINKQGGPHNVSFWPDSIPAGAQAVLNTAMTDQMAPLQS
                     QLLVDVDKTYDISFAGAPEGVYKFYCLPHLALGMHGKITVTK"
     gene            complement(2952..3803)
                     /locus_tag="VFU45_01815"
     CDS             complement(2952..3803)
                     /locus_tag="VFU45_01815"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5357827.1"
                     /translation="MAVTGGPARRYRLVDAITAGYAIIVAVVALREAPSAPGCWWVAG
                     GHLLILPLIWLVRRPGLGWLGRFVAEVYPIALITGLYTALDVLRGPTRPAVHDALIQR
                     WEAAIFGGQPSRDWWRTHPSLFWSTVLHGAYFMYYAIVPLPVLWFWFRKDLVALRRTV
                     LLEMTTFVFCYLWFVFFPVAGPYYEFARPTGAFIQTFTARLVYGALATGSSYGAAFPS
                     SHVAASVAAAGSAWLGDRRLGGVLFVFAGRLTVATVYCQMHYAVDATAGLLVGASAVW
                     LVARRKT"
     gene            complement(3794..>4627)
                     /locus_tag="VFU45_01820"
     CDS             complement(3794..>4627)
                     /locus_tag="VFU45_01820"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5357828.1"
                     /translation="VPGDLDDHTALRRAASGQDAIGHVAALTGAVDEAEFLHANRDGT
                     ANLLRAIEAAAPTARFVHVSSMAAGGPARRGRPRTEGEPDRPVTMYGRSKLAGEAATR
                     ASTLPWVILRPPTVYGPRDRDNLLVLFRAARRGVAPVFGDGSMELSAVHVGDLADAIV
                     RSFAAPAVAGQVFYVNHPEVVTSRQLVTEVAAAQGTRVRIIPLPEFLARAALGAIGTA
                     AALLRRKTILRYDKANEFYQEAWTADPSAFIAATGWAPAFDLRRGLADTCRWYREAGW
                     L"
BASE COUNT          708 a         1600 c         1712 g          607 t
ORIGIN      
        1 ggaggctcag cgtcgccacg ccggcggtgt cggtcggcac ggtcgtggcg gtgtagaggt
       61 tccagaccgg gcccgcgggt ccctcgatgg tgcggacggt ctggccatcg gacatctgct
      121 ggtcgacggc gaccaccggc gaggcctcgg gcccctcctg cagctggatc tggagcaccg
      181 ggagcccggc gacccgcgga atcggggtgc cggcggccgc cgcgcccgcg agcgacacgc
      241 tgtgccagag cccggccggc cccgccgccg gatcccggct cagcgaatcg tactccaccg
      301 tggcgcggat cgggtggtcg gccccgcgcc ggacgacgac ctgcggggtc cgcgcggccg
      361 ccacctgacg cgggacctcg gcgagcgggt gggccacggc cttcggggcg agcggggccg
      421 ggacgagcgg cgccggcagg aaccggcggg agccccatcc gagccccagg gccaggagga
      481 tgctcgcggc ccacgcggcg cgccgacgca cggcgcggcg cgtgcggcgc tctgccgcgg
      541 cccgccggac gacgtcgaac ggcgggagcg ggatcgtgcc gggcccgagt tcggcgagga
      601 gcgccgtcgt gcggtcgcgc agcagcgcac attcggcgcg tgccgcgcgg caccggtcgc
      661 aacgcgccag gtggctctcg atctcgaccg cctgcgagcg cgagagcgcc tggtcgaggt
      721 acgcgtggag ttcttcgtca ggaatgtgcc gcatcgatcc gttcgtgggt catccggtcg
      781 tgggcctcga cgagccgctt gcgggcccga gccagggtgg tgccgacggc gccgagcgcc
      841 aggccggtgc ggagcgagat ctcaggataa ctcaggcccg catcccagag gaggagcact
      901 tcctggtcgc gctcggtgag ggtggccagg gcccggtgca ccgacgccag ccgctcgtcg
      961 tgctccacca ggtcctgggc gtcgggctcg ggcgccggcg aaccggccgg gtcggaggtg
     1021 aggagcgtca ggtgtcgctt gcggcgcacc gccgcgcgcg cctcgtcccg cgccaggttg
     1081 gcagccacgg cgaagaccca ggcccgcggc ttctcgggct ggtggtgcat ggcccggacg
     1141 aagacctcct gcgccaggtc ctcggcccgg tcgaggtccc acaccttgcg gaagaggaac
     1201 ctgacgagcg acgtgtacgt cgattggtag agcgcctcaa ggtcgtcggt cattcggatc
     1261 ctgacgagag cggggggtga cggccctggc cgagaggccg cgtactgggc gattcaccag
     1321 cacctcagcg gacagggacc gtcgtcctgc gttccctcga cggtgatccc cgggggccgc
     1381 cccgggggcc gtcagtctta ggacgtccgg aacgtggtgc ggcgcacagg gcggcgctag
     1441 gccctgagcg cgtccgtccg gaagacgcac ccggcgtgaa acacttcacc atcgggccag
     1501 tcgaaacaga gttcgggctt ggccgggcgg ccatcggacc ggatccggcc gtggagggcg
     1561 cagctgcgcg catcgtcgtc gaggtacgcg caggacgaga tcatctcgac cccgagatag
     1621 cggtccgatt cccgatccac cttgcagagg tacggcgatc gccgggcccg caccagggcc
     1681 tccagtgcca cgtcgccacg gtcgcaccgg atgccgagct gcaggtcgcg gcagcaggcg
     1741 gccccgcggg tgttgtcggc ggtccggcac ggcgcggcga ggcacggatt ggtcccggcg
     1801 tagaccggga ggctggcggg tctcggcgcg agcgggagtg tgcgccgtgc aagggcgagt
     1861 ccttccacca cctcgaactc cgagtccccc aggcgccgcg gtgcgggtgt gcgggtcgtc
     1921 ggagccggtg ccggcccccg gtgcccgtgg gccttgcaga tgtcctcccc cgggccttca
     1981 cgcaggtggc gacaggggac gtggagcgtg gcctcggcga acaggctctg ctcttcccgc
     2041 cagacccgcc cgaaggtgat gttcccggtg tggccgagga gccacgtcgc ctcgtcgtcg
     2101 aggctcgctt ccccgtcctg cagccccccg acgcggaggg tcacggacag gtcccggcac
     2161 tgggggccaa gggcggcaca gggtgggatc ggagtcaggg gcacctcccc ctcgcgtctg
     2221 gcggggtggg ggattatgtt tgtgaaacca ttcacaaggg ttcccggccc gggtggcggg
     2281 ccgcggatgg tcgacagacc acgacaatct atcaggaatc ggaggcggtt gacgtatgcg
     2341 tgcgacttgg cttgcgactg gcctggtact ggtggcagcg tgtggcggtg gcgagaagaa
     2401 ggctgagacc ccggcggcgg ccccggccgc agccgcggcg gctccggctg cagctccggc
     2461 gacgaacgcc ccggtgcatg tggtcgagat gacgctcgat ggcacgaccc cgaagtacaa
     2521 cccgtccgag ctcacgatca agcagggtga aatcgtgcgc ttcatcaata agcagggcgg
     2581 cccgcacaac gtctccttct ggcccgacag catcccggcc ggcgcgcagg cggtgctcaa
     2641 caccgcgatg acggaccaga tggccccgct ccagagccag ctcctggtcg acgtcgacaa
     2701 gacctacgac atctcgttcg ccggtgcccc ggaaggcgtc tacaagttct actgcctccc
     2761 gcacctcgcg ctcggcatgc atgggaagat cacggtcacg aagtaagaag taggaagtaa
     2821 aaagtgagaa ggggctccgg tgaaggccgg ggcccctttt cttgcctcag accccgcctc
     2881 acgtcgatga catcggcacc tctccccgcc cacatctcac ctcttacgcc tgacgtctca
     2941 cgtctcatgt cctacgtctt acgtcttgcg accaaccaca cggccgacgc acccaccagc
     3001 agtccagccg tggcgtcgac ggcatagtgc atctggcaat agacggtggc cacggtgagc
     3061 cgcccggcga agacgaacag cacgccgccc aggcgacgat cgcccagcca cgccgacccc
     3121 gccgcggcga cggacgccgc cacgtgcgag gacgggaacg cggcgccgta tgagctcccc
     3181 gtcgcgagcg ccccatacac caggcgggca gtgaaggtct ggatgaacgc ccccgtgggc
     3241 cgggcgaact cgtagtatgg gcccgccacc gggaagaaga cgaaccagag gtagcagaac
     3301 acgaacgtcg tcatctcgag gagcacggtc cgccgaagcg ccacgagatc cttccggaac
     3361 cagaaccaga gcaccggcag cggtacgatc gcgtagtaca tgaaatacgc gccgtgcagc
     3421 acggtggacc agaagaggct cggatgtgtt cgccaccagt cccggctcgg ctgaccgccg
     3481 aagatcgcgg cttcccaccg ctggatgagc gcgtcatgca ccgccgggcg ggtcgggccg
     3541 cgcagcacgt cgagcgcggt gtagagtccg gtgatgaggg cgatcgggta cacctcggcc
     3601 acgaaccggc cgagccagcc gagcccggga cgacgcacca gccagatgag gggcaggatg
     3661 aggaggtggc ccccggccac ccaccagcac cctggggcgg agggggcctc ccggagcgcc
     3721 accaccgcca cgatgatcgc gtagccggcg gtgatcgcat ccaccagccg ataccggcga
     3781 gcgggcccgc cggtcacagc caccccgcct cgcgatacca ccggcaggtg tcggccaggc
     3841 ctcgccgcag gtcgaacgcg ggcgcccagc cggtggcggc gatgaaggcc gacggatcgg
     3901 cggtccacgc ctcctggtag aactcgttgg ccttgtcgta gcgcaggatg gtcttccggc
     3961 ggaggagggc ggcggcggtg ccgatggcgc cgagtgccgc gcgcgccagg aactccggga
     4021 gcgggatgat gcgcacccgg gtgccctggg ccgccgcgac ctcggtcacc agctgccggc
     4081 tggtgaccac ctcggggtgg ttgacgtaga acacctggcc ggcgaccgcc ggggccgcga
     4141 agctccggac gatcgcgtcc gccaggtcgc ccacatggac ggccgagagc tccatcgaac
     4201 cgtcgccgaa caccggggcc acgccccgcc gggcggccct gaacaggacg aggaggttat
     4261 cgcggtcgcg ggggccgtac accgtgggcg gacggaggat gacccatggc agcgtggagg
     4321 cgcgagtggc ggcctcccct gcgagcttgc tgcggccgta catcgtgacc ggccggtcag
     4381 gctcaccttc ggtgcggggt cggccgcgcc gcgccgggcc gccagctgcc atcgagctca
     4441 cgtggacgaa gcgggccgtc ggcgccgccg cctcgatggc ccggagcagg ttggcggtgc
     4501 cgtcacggtt ggcgtggagg aattccgcct cgtcgacggc gccggtgagc gccgccacat
     4561 ggccgatggc gtcctggccc gaggccgcgc gccggagggc cgtgtggtcg tcgaggtcgc
     4621 cggggac
//
LOCUS       DASUAP010000073         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1010409, whole genome shotgun sequence.
ACCESSION   DASUAP010000073 DASUAP010000000
VERSION     DASUAP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010409"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFU45_01825"
     CDS             complement(<1..767)
                     /locus_tag="VFU45_01825"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5357829.1"
                     /translation="MSAPRRGPRGFTLVETLIALVLLGLVSTVIYKMLITTQRVTDAQ
                     AQRGDLQDNLRSGALVIPNELRQITIGTSSSLDTISDIRAVSDTSITYRAMRGYYTIC
                     ATPAVATTLTVTNASGFSSEYRAPTTSDSAFVFWEGDTTKTSDDAWVQAQIVGVSSGT
                     CTYAGASRSAYTFTLVAPALASTSFPYTKIQAGSPVRTYETVTLSLYTSGGQQWLGMK
                     VGSGTMQPVVGPLAATSGGLYGFQLTYYDSTGAVLSA"
     gene            complement(764..1138)
                     /locus_tag="VFU45_01830"
     CDS             complement(764..1138)
                     /locus_tag="VFU45_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5357830.1"
                     /translation="MTNDRAGFTITEVMIAVVILGIGVLALAGGSAAATRMLGQGQRT
                     TNATAVAAARIERLRRAANATNPRCTSGSFASGTATTNNVSEAWTVPTSGIARTVTEV
                     VTYKKYNGTITDTLATIVSCAT"
     gene            complement(1135..1632)
                     /locus_tag="VFU45_01835"
     CDS             complement(1135..1632)
                     /locus_tag="VFU45_01835"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEU5357831.1"
                     /translation="MFSRSMGSAMVRSRHGFSLIEVVIVLALIAIITTIAVPALSTSS
                     DAKKVKGAANSVASQLGIARTAAIARDRCATVHLNSSGKLWVTTQTCGGSPIDTLTSR
                     SLATAYGVTATACSGSSCSPGSSLDYAFDPRGIPYTQSPATYVLTRNTAADTVNVGQF
                     GRVTR"
     gene            complement(1749..3089)
                     /locus_tag="VFU45_01840"
     CDS             complement(1749..3089)
                     /locus_tag="VFU45_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5357832.1"
                     /translation="MTRVLIIDDDPGLRHSLGLLLGEAGYAVVAEGDPARALELASTD
                     PFDLILCDVRMPGLDGREFLRRYRAAGGTALVVVMSAYGSEDAALEVIREGAYDYLHK
                     PFRPDEVLFTLRKAEERERLRREVAELRATVAGGAAGGGLVVESPAMRQVVELASRVA
                     GHATTVLLTGESGSGKEVIARLIHQRSPRAAERFVAVNCAAIPEALIESELFGHVRGS
                     FTGATGDRRGLFEEADRGTLLLDEIADLPFALQPKLLRALEEGAIRRVGEASERKVDV
                     RVIAATARPLDQLVRDGRFREDLFYRLNVVQLALPPLRDRPEDIPALAAHFLAQAGAR
                     LGRPLSLSPDALGRLLGYRWPGNVRELRNVIERAAVLAPSATLDAGDFLLGDTRGSAP
                     AGTTDSLKPQVEAIERAAIQRALAGADGNRRAAAKALGVSLRTLFYKIRRYGLE"
     gene            complement(3086..4213)
                     /locus_tag="VFU45_01845"
     CDS             complement(3086..4213)
                     /locus_tag="VFU45_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5357833.1"
                     /translation="MRRSLRGELLFSFAFLTSAAVLLVGASATLLAGHDAGAAVWALL
                     FLWLGSTGVIVVFGAWLLRGRVLRPLRLLGEQADALAEGRSPAPAAFDTADFAHVSER
                     LTWMADRLLDARSQVVRAEKLAAVGQLAAGVAHEIRNPLGALGNYLEVLRRRGGDAAV
                     LADMQREVERMDRIVQELLDYARPRATPAPIDVGAAVRATVELLGAQRTVEPARLHLD
                     VAADLPPVRVERHAVEQLVVNLVLNARDARPDGRIWVGVGRATSGPAPEPRRAEDPAG
                     DDRRPAREREFRPWRADRPAGAPGVALHVADEGPGIPEGDRDRVFDLFYTTKAPGAGT
                     GLGLAIAARTVHDAGGVIWVDRGREGGAVFRVFLPAEEVPA"
     gene            complement(4272..>4916)
                     /locus_tag="VFU45_01850"
     CDS             complement(4272..>4916)
                     /locus_tag="VFU45_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357834.1"
                     /translation="WAANCASLVRSNQPAIRSSSSISTGGPKYYITPSSSTSDTSVAT
                     SVAKVNGLFTTLAGMADLSFTSATVPSSIAPVLSGHTCAEGSSNAGNWGDPTYAASGV
                     DGTSHPCAGYFPVIYVNGNTTLPTGSGQGVLLINGNLTFGSSFKFYGVVLVTGTAKAT
                     GGSNGNVYGALLAESGTSNLTGNERFDYSSCAVTLAAEASLIGRPLSVRPYIRF"
BASE COUNT          723 a         1692 c         1783 g          718 t
ORIGIN      
        1 ctggccgaga gcacggcccc ggtcgagtcg tagtaggtga gctggaagcc gtagaggcca
       61 ccgctcgtgg cggcgagcgg tcccaccacc ggctgcatcg tgccgctccc caccttcatg
      121 ccgagccact gctgcccgcc cgaggtgtag agcgagagcg tcaccgtctc gtacgtccgg
      181 accggcgagc ccgcctggat cttggtgtac gggaaagacg tcgaggccag cgccggggcc
      241 accagcgtga acgtgtacgc cgaccgggag gcacccgcgt acgtgcacgt gccgctcgac
      301 accccgacga tctgggcctg cacccaggcg tcgtcggagg tcttggtggt atccccctcc
      361 cagaagacga acgcgctgtc gctggtggtc ggggcccggt actccgagga gaaaccggag
      421 gcgttggtca cggtgagcgt ggtggccacg gccggtgtgg cgcagatggt gtaataaccc
      481 cgcatggcgc ggtaggtgat cgatgtgtcg ctcaccgccc ggatgtccga gatcgtgtcg
      541 aggctgctcg aggtgccgat ggtgatctgg cgcagctcgt tcgggatgac cagcgcgccc
      601 gagcgcaggt tgtcctggag gtcgccgcgc tgggcctgcg cgtcggtgac ccgctgggtg
      661 gtgatgagca tcttgtagat gaccgtcgag accaggccca ggagcacgag cgcgatgagc
      721 gtctcgacca gggtgaagcc gcggggacca cgccgggggg cgctcatgtg gcacagctca
      781 cgatcgtggc gagggtatcg gtgatggtcc cattgtactt cttgtaggtg accacctcgg
      841 tcaccgtccg ggcgatgccg gacgtcggca cggtccaggc ctcggacacg ttgttggtgg
      901 tggcggtgcc gctggcgaag gatcccgacg tgcaccgcgg gttggtcgcg ttggcggccc
      961 ggcggagccg ctcgatgcgg gccgccgcga ccgccgtggc attggtggtg cgctggccct
     1021 ggcccagcat gcgcgtcgcc gccgccgagc cgccggcgag cgccaggacg ccgatgccga
     1081 ggatgaccac ggcgatcatc acttcggtga tggtgaagcc ggcacgatcg ttcgtcatcg
     1141 cgtgaccctc ccgaactggc cgacgttgac ggtgtcggcg gcggtgttcc gcgtcaggac
     1201 gtaggtcgcc ggactctggg tgtaggggat gccccggggg tcgaaggcgt agtcgagcga
     1261 gctccccggg ctgcacgaac tcccgctgca cgcggtcgcc gtcacgccgt aggccgtggc
     1321 caggctgcgg ctggtgagcg tgtcgatcgg cgagccgccg caggtctgcg tcgtgaccca
     1381 gagcttgccg ctgctgttga ggtgcaccgt ggcgcagcgg tcccgggcga tcgccgcggt
     1441 gcgcgcgatg cccagctggc tcgcgacgga attggcggcg cccttcacct tcttcgcgtc
     1501 gctgctcgtg ctgagcgccg gcaccgcgat cgtcgtgatg atcgcgatga gcgcgagcac
     1561 gatcaccacc tcgatcaggc tgaaaccgtg ccgcgatcgt accatcgctg accccattga
     1621 acgagagaac acgccgccct cctaggcagg atgggtgccg ctatctaagc tattgtcaaa
     1681 caacaggtta taacttgggg tgcaatgctt tggcatgcaa tgagatgcac atcgtgcaat
     1741 tatcggcgct attccagccc atatcggcgg atcttgtaga acagggtccg gagactcact
     1801 cccagcgctt tcgccgcggc ccgccggttg ccgtccgcgc cggccaacgc ccgttggatg
     1861 gcggcccgct cgatcgcctc gacctgcggc ttgagcgagt cggtggtccc ggcgggcgcc
     1921 gagccccgcg tgtcgcccag caggaagtcg cccgcatcca gggtcgcgct cggcgcaagc
     1981 acggcagcac gctcgatgac gttccggagc tcccgcacgt tgccgggcca ccggtagccg
     2041 aggagccggc cgagcgcgtc cggactcagg ctcaggggcc gaccgagccg ggcgccggcc
     2101 tgcgccagga agtgcgccgc gagcgcgggt atgtcctccg gccggtcgcg cagcggcggc
     2161 agcgcgagct gcaccacgtt gagccggtag aagaggtcct cccggaatcg gccgtcccgc
     2221 accagctggt cgaggggccg cgcggtcgcc gcgatgacgc gcacgtcgac cttccgctcg
     2281 ctcgcttcgc ccacccggcg gatggccccc tcttccaggg cccgcaagag cttcggctgc
     2341 agcgcgaagg ggaggtcggc gatctcatcg agcaggagcg tcccccgatc ggcctcctca
     2401 aagagcccgc ggcggtcccc ggtggcgccg gtgaacgagc cgcggacgtg gccgaagagc
     2461 tccgactcga tcagcgcctc gggaatggcg gcgcaattga ccgcgacgaa gcgctccgcc
     2521 gcccgcgggc tccgctggtg gatgagcctt gcgatgacct ccttgccgct cccgctctcc
     2581 ccggtgagga gcacggtcgt ggcgtggccg gccacccggc ttgccagctc gaccacctgc
     2641 cgcatcgccg ggctctcgac cacgagaccc ccgcccgccg caccccccgc caccgtggcg
     2701 cgcagctcgg ccacctcgcg ccggagccgc tcgcgctcct cggccttccg cagtgtgaag
     2761 aggacttcgt cgggccggaa gggcttgtgc aggtagtcgt acgcgccttc gcggatgacc
     2821 tcgagcgccg cgtcctcgct cccgtaggcg ctcatcacca cgacgagcgc ggtcccgccg
     2881 gcggcgcgat accgtcgcag gaattcacgt ccatcgagcc cgggcatccg gacgtcgcaa
     2941 aggatgaggt caaagggatc ggtgctcgcc agctccaggg cccgcgcggg atcgccctcg
     3001 gccacgacgg cgtatccggc ctcgccgagc aggagcccga gcgaatgccg gaggccggga
     3061 tcgtcgtcga tgatcagcac ccgcgtcacg ccggcacctc ctcggcgggg aggaacaccc
     3121 ggaacaccgc accgccctcg cggccacgat ccacccagat gacgccgccg gcatcgtgca
     3181 ccgtccgcgc cgcgatggcg agaccgagcc cggtgccggc gccaggcgcc ttcgtggtgt
     3241 agaagaggtc gaacacgcgg tcacggtcgc cctcgggaat gccgggacct tcgtcggcca
     3301 cgtggagcgc gactcccggg gcgccggcag gccggtcggc ccgccagggg cggaactccc
     3361 gttcgcgcgc cggtcgccgg tcatcgcccg ccggatcctc ggcgcggcgc ggttccgggg
     3421 ccggccccga cgtggcccgt cccacgccga cccagatcct tccgtcgggt cgggcgtccc
     3481 gggcattgag cacgaggttc accacgagtt gttccacggc gtggcgctcg acgcggacgg
     3541 gcggaaggtc ggcggcgacg tcgaggtgga gccgggccgg ctcgacggtg cgctgggcgc
     3601 cgaggagttc caccgtcgcg cggacggcgg cgcccacgtc gatcggcgcc ggcgtcgccc
     3661 gggggcgcgc gtagtccagc agctcctgga cgatccggtc catccgttcc acctcgcgct
     3721 gcatgtcggc cagcaccgcg gcgtcgccac ctcggcggcg gagcacctcg aggtagttcc
     3781 ccagcgcgcc gagggggttc ctgatctcgt gggccacgcc ggcggcgagc tgacccaccg
     3841 ccgccagctt ctcggcgcgg accacctggc ttcgggcgtc gaggaggcgg tcggccatcc
     3901 aggtgaggcg ctcgctcacg tgggcaaagt ccgccgtgtc gaacgcggcc ggggcgggcg
     3961 agcgtccctc cgccagggca tcggcctgct cgccgaggag gcggaggggg cggagcaccc
     4021 ggccccgcag aagccaggcg ccgaagacca cgatgacccc ggtgctcccg agccagagga
     4081 agaggagcgc ccacaccgcc gccccggcgt cgtgtccggc caggagcgtg gcgctcgccc
     4141 ccaccagcag caccgccgcc gaggtgagga acgcgaagct gaagaggagc tcgccgcgaa
     4201 gggagcggcg cacgggtggc atgtctgtaa agatgggacg gcttgccggc gggcgcgagg
     4261 gcgcgccggc atcagaaccg gatgtagggc cggacggaga gggggcgccc gatgagcgac
     4321 gcctcggcgg cgagggtcac ggcgcagctc gagtagtcga accgctcgtt gccggtcagg
     4381 ttcgaggtgc cggattccgc gagcagggcg ccatagacat tcccgttcga tccgccggtt
     4441 gccttggccg tgccggtgac gagcacgacc ccgtagaact tgaaggacga cccgaatgtg
     4501 aggttgccgt tgatgagcag gaccccctgc ccgcttccgg tggggagggt cgtgttgccg
     4561 ttgacgtaaa tgaccgggaa gtagccggca cacggatgac tggtgccgtc gacgccggat
     4621 gccgcatagg tcgggtcgcc ccagttcccg gcattggacg acccctcggc acaggtgtgt
     4681 ccgctcagca ccggcgcgat cgacgaaggc accgtggccg aggtgaaact gaggtcggcc
     4741 attccggcga gcgtcgtgaa gagcccgttc accttggcga ccgatgtcgc gaccgacgta
     4801 tcgctcgtgg aactcgacgg ggtgatgtag tacttcggtc cgccggtaga aatgctggaa
     4861 ctggagcgga tcgccggctg attgctccgc accagggacg cacagttggc ggccca
//
LOCUS       DASUAP010000074         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_846793, whole genome shotgun sequence.
ACCESSION   DASUAP010000074 DASUAP010000000
VERSION     DASUAP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846793"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFU45_01855"
     CDS             complement(<1..510)
                     /locus_tag="VFU45_01855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEU5357835.1"
                     /translation="MITLLAPPGSLTLGASVSLDATEAHHLRVRRGEAGELLRLVDGL
                     GRIGYGAVTLGTHGAYVTLEKVEDAPIPPRLVLAVGAGEKDRFAWLAEKATELGVTDL
                     VPIECERTVSVASRVRDGAIERIQRRATEALKQCEGTWAPKIHGNTPLERFLAEAPPG
                     RRWVMDQAGV"
     gene            complement(507..1337)
                     /locus_tag="VFU45_01860"
     CDS             complement(507..1337)
                     /locus_tag="VFU45_01860"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU5357836.1"
                     /translation="MTLWALAVRVDAGRRDALAAWLVARTGQAVEERADGMVVAYVED
                     AAVEALADGVRAREGNTVPVERSPVPPVDWVSHWRDGIGARTVGRILLAPSWIEARPA
                     AGQVRLTLDPETAFGSGEHGSTRGALALLDRHLKPGARVLDLGSGSGILAIAAALLGA
                     SRAVGIELDGDAVPIAQSNADKNGVSDRVVFIHGDAGRSAALGAPADVVCANILRNIN
                     VSLLPAITDAMAPGAVAIFAGMEETEEVLFRPQLAAAGFTAVDEARDEGWWSVAARRA
                     "
     gene            complement(1334..2476)
                     /gene="dnaJ"
                     /locus_tag="VFU45_01865"
     CDS             complement(1334..2476)
                     /gene="dnaJ"
                     /locus_tag="VFU45_01865"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU5357837.1"
                     /translation="MSDHYRLLGVARDATEADLKKAYRKLAMEFHPDRNREPGAEEKF
                     KEITEAYEILRDPDKRAAYDRYGDAAFKAGGMGGSGGFHHVDLSEALGIFMRDFGGMG
                     GFDAFFGGGGRAQTEARRGQDVRVSLKLTLAEVATGVRRTIKLKTLATCDACGGSGAK
                     AGSKPVTCGTCGGAGQVRRAAQSIFGQFVSVSPCPTCGGEGTVIKTPCESCRGEGRVK
                     AERTVNVDVPAGIAENNYITLRGQGAAGPRRGIAGDLLVGIEIEPDERWERQGDDLLH
                     DLPLSFAQAALGGSFKVPSPYGEEKVTVPAGTQTGTVFKLRGKGLPRLQQEGKGHLLV
                     RTHVWTPDELSDEQRRLFQEIAKLEGEPPRKVTHGFWSRLKESLGA"
     gene            complement(2473..>3492)
                     /gene="hrcA"
                     /locus_tag="VFU45_01870"
     CDS             complement(2473..>3492)
                     /gene="hrcA"
                     /locus_tag="VFU45_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU5357838.1"
                     /translation="DQLSDREVRVLEAVIQTYIETAEPAGSQTIAKRFGLGVSSATIR
                     STMSDLEEKGFLFHPHTSAGRIPTDRAYRLYVDHLMHPAPVQPGVRSALELELPARSA
                     VDGLLRRAAEVLSVLTEELGVAAAPAVESAVLERLELVQVASDRLLLVFNLKSGVVRT
                     IFVHAQAVVAANAVEQVARILNERLAGLTLREIRASLAERLRDTRAAGGERDLLNIFI
                     AEGDEIFSVGASSDAVVLGSAQKLADQPEFASNERMRELLTLTERRDLIKQALATREH
                     GLTITIGGEHGDKDLAGFTLVTSTYRVGDLRGVIGVLGPTRMPYEKVIGLVQHTSRLV
                     EGLLQ"
BASE COUNT          446 a         1280 c         1251 g          515 t
ORIGIN      
        1 gacgccggcc tggtccatca cccatcggcg tcccggcggc gcctccgcga ggaagcgctc
       61 gagcggggtg ttgccgtgga tcttgggggc ccaggtccct tcgcactgct tgagtgcctc
      121 ggtggcgcgg cgctggatcc gctcgatggc cccgtcccgc acccgcgagg cgacgctcac
      181 cgtgcgctcg cactcgatcg gcacgaggtc ggtgacgccg agctcggtgg ccttctccgc
      241 cagccaggcg aagcgatcct tctcccccgc tcccaccgcg agcaccaggc gcggcgggat
      301 cggcgcatcc tcgaccttct cgagcgtcac ataggcgccg tgggtgccga gcgtcacggc
      361 gccgtagccg atgcggccca ggccgtcgac cagccggagg agttcccccg cctcgccccg
      421 ccgcacccgc aggtgatgcg cttcggtggc gtcgagcgac accgatgcgc ccagcgtaag
      481 actcccgggc ggggcgagga gggtgatcat gcgcgcctcg ccgccaccga ccaccacccc
      541 tcgtcccgcg cctcgtcgac cgcggtgaac cccgccgccg cgagctgggg ccggaagagg
      601 acctcttcgg tctcttccat cccggcgaag atcgcgaccg caccgggggc catggcgtcg
      661 gtgatcgctg gcaggaggct cacgttgatg ttccggagga tgttggcgca gacgacgtcg
      721 gccggggcgc cgagcgctgc ggagcgtccc gcgtcgccgt ggatgaagac gacccggtcg
      781 gacacgccgt tcttgtcggc attggactgg gcgatcggca ccgcgtcgcc gtcgagctcg
      841 atgcccacgg cgcgggaggc gccgaggagt gccgcggcga tggccaggat cccgctcccg
      901 ctcccgaggt cgagcacgcg ggcgccgggc ttgaggtggc ggtcgaggag tgccagcgcg
      961 ccgcgggtcg agccgtgctc accgctgccg aaggcggtct ccggatcgag cgtcaaccgc
     1021 acctgccccg ccgccgggcg ggcctcgatc cagctcggcg ccagcaggat gcgcccgacg
     1081 gtgcgggcac cgatcccgtc gcgccagtgc gacacccaat cgaccggcgg caccggggag
     1141 cgctcgaccg gcaccgtgtt cccttcgcgg gcccggaccc catcggcgag cgcctcgacc
     1201 gcagcgtcct cgacgtaggc gaccaccatc ccgtcggccc gctcttccac cgcctggccg
     1261 gtccgggcca cgagccacgc ggccagcgcg tcgcgccgcc ccgcgtccac ccgcaccgcg
     1321 agcgcccaga gcgtcacgcg ccgagcgatt ccttgagccg cgaccagaag ccgtgggtca
     1381 ccttccgggg cggctcccct tcgagcttgg cgatctcctg gaagagccgg cgctgttcgt
     1441 cggagagttc gtccggggtc cagacgtggg tgcgcaccag caggtgcccc ttcccttcct
     1501 gctgcaggcg cggcagcccc ttgccgcgga gcttgaacac cgtgccggtc tgggtgcccg
     1561 cggggacggt caccttctcc tcgccgtagg gcgaggggac cttgaacgag ccgccgagcg
     1621 ccgcctgcgc aaaggagagc ggcaggtcgt ggagcaggtc gtcgccctgg cgctcccagc
     1681 gctcgtcggg ctcgatctcg atgccgacga ggaggtcgcc ggcgatgccg cgccgggggc
     1741 ccgccgcccc ctggccccgc agcgtgatgt agttgttctc cgcgatcccc gccgggacat
     1801 ccacgttgac ggtccgctcc gccttgaccc gcccttcgcc tcggcacgac tcgcagggcg
     1861 tcttgatgac cgtcccctcg ccgccgcagg tgggacaggg cgacaccgac acgaactgcc
     1921 caaagatgct ctgcgcggcg cgtcgcacct gacccgcgcc gccgcaggtg ccgcaggtga
     1981 ccggcttgga gccggccttg gcgccgctcc cgccgcaggc gtcgcaggtg gccagggtct
     2041 tgagcttgat cgtgcgccgc acgccggtgg ccacctcggc gagcgtgagc ttgaggctca
     2101 cccgcacgtc ctggccccgc cgcgcctccg tctgcgcgcg cccgccgccg ccgaagaagg
     2161 cgtcgaagcc ccccatcccg ccgaagtcgc gcatgaagat gccgagcgcc tcggagaggt
     2221 ccacgtggtg gaacccgcct gagcccccca tcccgccggc cttgaacgcc gcatcgccgt
     2281 accggtcgta ggcggcgcgc ttgtccggat cgcggaggat ctcgtacgcc tcggtgatct
     2341 ccttgaactt ctcctccgcc cccggttccc ggttccggtc ggggtggaac tccatcgcga
     2401 gcttgcggta ggccttcttg aggtcggcct cggtcgcgtc gcgcgcgacg cccaggagcc
     2461 ggtagtggtc gctcattgga ggagcccttc caccaggcgg ctggtgtgct ggacgaggcc
     2521 gatgaccttc tcgtacggca tccgggtggg gccgaggacg ccgatcacgc cccggaggtc
     2581 gcccacgcga taggtcgagg tgacgagggt gaagcccgcc aggtccttgt cgccgtgctc
     2641 accgccgatg gtgatcgtga gcccgtgctc ccgggtggcg agcgcctgct tgatgaggtc
     2701 gcgccgctcg gtgagcgtga ggagttcccg catccgttcg ttggaggcga attccggctg
     2761 gtcggcgagc ttctgcgcgc tgcccagcac cacggcgtcg gatgatgcgc cgacggagaa
     2821 gatctcgtcg ccttcggcga tgaagatgtt gaggaggtcg cgctccccac cggcggcgcg
     2881 ggtatcgcgc aggcgctcgg ccagcgacgc gcggatctcc cgcagggtga gcccggccag
     2941 ccgttcgttg aggatccgcg ccacctgttc cacggcgttg gcggccacca ccgcctgggc
     3001 gtggacgaag atcgtccgga cgacgccgct cttgaggttg aagacgagga ggagccggtc
     3061 gctcgccacc tggaccagct cgagccgctc gagtaccgcg ctctccaccg ccggcgccgc
     3121 ggcgacgccg agctcctcgg tcaggacgct cagcacctcg gcggcgcggc ggaggagccc
     3181 gtcgaccgcc gagcgggcgg ggagctccag ctccagcgcg gaccggacgc cgggctgcac
     3241 cggtgccggg tgcatcaggt ggtcgacgta gaggcggtac gcgcggtcgg tcgggatccg
     3301 gccggcgctg gtgtgcgggt ggaagaggaa gcccttctct tccaggtcgc tcatcgtgct
     3361 gcggatcgtg gccgaggaga cgccgagccc gaagcgcttg gcgatcgtct ggctgccggc
     3421 gggctccgcg gtctcgatgt aggtctggat gaccgcttcg aggacccgga cctcgcggtc
     3481 ggagagctgg tc
//
LOCUS       DASUAP010000075         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1146486, whole genome shotgun sequence.
ACCESSION   DASUAP010000075 DASUAP010000000
VERSION     DASUAP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146486"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /gene="murJ"
                     /locus_tag="VFU45_01875"
     CDS             complement(<1..1411)
                     /gene="murJ"
                     /locus_tag="VFU45_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU5357839.1"
                     /translation="MLVGMGILASRLVGLVRTKVIAYYLGAGVAADAWQAAFKIPNFL
                     QNLFGEGALSASFIPVYAGLLAQDRKKEADDVAGAVGGLLALAMAVVVAIGVVTTPWL
                     IDLVVPGFTGEKRTLTIELVRIVFPGTGLLVLSAWCLGVLNSHRRFFLSYAAPVLWNL
                     TIIVTLLFFGRRVAEFELAVVAAWASVVGSALQVLVQWPRVLRLLGGFRPSLDHGSAH
                     VREVIRNFGPAFVSRGVVQISSYIDVFLASPLPTGAVAHLSYAQTLFILPVSLFGMSV
                     SAAELPAMSSALGSTEEIAGQLRVRLEGGLREIAFFVVPSAMAFLALGRIIARVIFQA
                     GHFTAADSITVWAVLCGAAIGLLATTQARLYSSAFYALRDTRTPLNIGILRVLLVTAL
                     GYAAAFPIREALGIDGRWGPVGLTASAGIAGWVEFLLLQRALGRRIGPTGIHLAELAR
                     LWVAAAVAAGAAWGAIEALG"
     gene            complement(1441..1722)
                     /locus_tag="VFU45_01880"
     CDS             complement(1441..1722)
                     /locus_tag="VFU45_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357840.1"
                     /translation="MVILGAVLLAVLIIPVLAILFESPLGRAFARRVAGPEPVTGTLA
                     ELAKKVELLEGEVDDLHRAVELLQEENQFLQRLVEDAPRPPTLPPGPRD"
     gene            complement(1731..>2240)
                     /gene="bshC"
                     /locus_tag="VFU45_01885"
     CDS             complement(1731..>2240)
                     /gene="bshC"
                     /locus_tag="VFU45_01885"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HEU5357841.1"
                     /translation="RPVPRWSGLVIEPFVDRLLPRLEVTLDEIVTDGAAAEARIARRQ
                     LPPDVLAALGAMRDRIGASYDLLARVAPAIDPTLVRAMQGLRDRSLFDAERAERKLVR
                     AFKRKGSTELRQLDRLRATLRPADAPQERILTVAPWLARYGAGFLPALAAAVEQWYDG
                     ALEPHSPAR"
BASE COUNT          385 a          770 c          816 g          269 t
ORIGIN      
        1 cgccgagggc ctcgatggcg ccccacgccg ctcccgcagc cacggcggcc gcgacccaga
       61 gccgggccag ctcggccaga tggatacccg tagggccgat ccggcgccca agggcccgct
      121 ggagcaggag gaactcgacc catcccgcga tcccggccga ggcggtgagc ccgaccgggc
      181 cccagcggcc gtcgatgccg agcgcttccc ggatcgggaa cgccgccgcg tagcccaggg
      241 cggtcaccag cagcacccgc aggatcccga tgttgagcgg ggtccgggtg tcgcgcaggg
      301 cgtagaatgc cgacgaataa agtcgtgcct gggtggtcgc caggaggccg atggcggcgc
      361 cacagagcac cgcccagacg gtgatgctgt cggccgcggt aaagtggcct gcctggaaga
      421 tgacccgcgc gatgatccga cccagcgcca ggaatgccat cgccgatggc acgacgaaga
      481 acgcgatctc ccgcaggccg ccttccagcc ggacacggag ctggcccgcg atctcctcgg
      541 tgctgccgag cgcgctcgac atcgccggca gttccgccgc cgagaccgac atcccgaaga
      601 ggctcacggg caggatgaag agcgtctggg cataggagag atgggccacc gcgccggtcg
      661 ggagcgggct tgccaggaag acgtcgatgt aggagctgat ctgcacgacg ccccggctca
      721 cgaaggcggg accgaagttc cggatgacct cgcgcacgtg cgccgacccg tggtcgagcg
      781 agggccggaa ccctccgaga agccgcagga cccgcggcca ctgtacgagg acctggagcg
      841 cgctcccgac cacggacgcc caggcggcca cgacggccag ctcgaattcc gccacgcggc
      901 gcccgaagaa cagaagcgtc acgatgatcg tgaggttcca gagcacgggc gcggcgtacg
      961 acaggaagaa gcgccgatgg ctgttgagga ccccgaggca ccaggccgag agcacgagga
     1021 ggccagtgcc ggggaagacg atgcggacga gctcgatggt gagggtgcgc ttttcgccgg
     1081 tgaatccggg gaccaccagg tcgatcagcc agggcgtggt gacgacgccg atggcgacga
     1141 cgaccgccat cgcgagcgcc agcaatcctc cgacggcacc ggcgacgtca tcggcctcct
     1201 tcttccgatc ctgcgcgagc aggccggcat agacggggat gaaggacgcc gagagcgcgc
     1261 cctcgccgaa gaggttctgg aggaagttcg ggatcttgaa tgcggcctgc cacgcgtccg
     1321 cggcgacgcc ggcgccgagg tagtacgcga tgaccttcgt gcggaccagc cccaccaggc
     1381 ggctggccag gatccccatg ccgaccagca ccgcgccgcg gcggatggca cgttcggagt
     1441 tcagtcgcga ggtcctggcg ggagggtggg cggccggggg gcgtcttcga cgaggcgctg
     1501 gaggaactgg ttctcctcct ggagtagttc gaccgcacgg tgcaggtcat cgacctcgcc
     1561 ctcgaggagc tcgaccttct tggccaactc cgccagcgtg cccgtcaccg gttccgggcc
     1621 ggcgacgcgg cgggcgaagg cccgcccgag cggcgattcg aacaggatcg ccagcaccgg
     1681 gatgatgagc accgcgagga gtaccgcgcc gaggatgacc atgcgggagt ctatcgggcg
     1741 ggggaatgcg gttcaagtgc gccgtcgtac cattgctcga cggcggccgc cagcgccggg
     1801 aggaaccccg cgccgtaccg ggccaaccac ggcgccacgg tgaggatccg ctcctgcggc
     1861 gcgtcggcgg gtcgaagcgt cgcccgcaac cggtcgagct ggcgcagttc cgtcgaccct
     1921 ttccgcttga aggcccggac gagcttccgc tcggcacgct ccgcatcgaa caggcttcgg
     1981 tcgcgcagcc cctgcatggc gcggacaagc gtcggatcga tggccggcgc cactcgcgcg
     2041 aggaggtcgt acgaggcgcc gatccggtcc cgcatcgcgc cgagggccgc caggacgtca
     2101 ggcggcagct gccgtcgagc gatccgcgcc tcggccgcgg ccccatccgt gacgatctcg
     2161 tcgagcgtga cctcgagcct gggcaggagg cggtcgacga agggctcgat cacgaggccc
     2221 gaccaccgcg gcacgggacg
//
LOCUS       DASUAP010000076         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_533475, whole genome shotgun sequence.
ACCESSION   DASUAP010000076 DASUAP010000000
VERSION     DASUAP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533475"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..686)
                     /gene="rpsC"
                     /locus_tag="VFU45_01890"
     CDS             complement(27..686)
                     /gene="rpsC"
                     /locus_tag="VFU45_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU5357842.1"
                     /translation="MGQKTHPTGFRLGIIKPWQSRWFAGKDMPALLKEDELIRKYLKA
                     RLGHAAIAEVHIERRPGKVTVTVHTGRPGVVIGKRGAEVDKLRDELAQLTGKEAAINV
                     EEIKRPELAAQLVADNIAHQLEQRISFRRAMKRAVQSAMRMGAQGIKVRTSGRLGGAE
                     IARTEGYREGRVPLHTLRADVEYATSTARTTSGTIGVKVWIFKGEVVEDLGGRTYSTG
                     A"
     gene            complement(686..1042)
                     /gene="rplV"
                     /locus_tag="VFU45_01895"
     CDS             complement(686..1042)
                     /gene="rplV"
                     /locus_tag="VFU45_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU5357843.1"
                     /translation="MTGQAIQRGVRQSPRKMRLVMDLIRGKDVNEAYALLKFSKKLAA
                     RQIEKTLRSAVANAEQAALKDNEPFDVDVMVVSKAVVDMGQPLKRFTAAAQGRATPIR
                     KRTSHVEIHVAARETN"
     gene            complement(1045..1335)
                     /gene="rpsS"
                     /locus_tag="VFU45_01900"
     CDS             complement(1045..1335)
                     /gene="rpsS"
                     /locus_tag="VFU45_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU5357844.1"
                     /translation="MARSVKKGPFVQDALRRKVDGLNGRNEKKVVKTWSRASTILPEF
                     VGHTFAVHNGNKFGPVDVPENMVGHKLGEFAPTRLFRGHSGKIVADKKAKPE"
     gene            complement(1336..2175)
                     /gene="rplB"
                     /locus_tag="VFU45_01905"
     CDS             complement(1336..2175)
                     /gene="rplB"
                     /locus_tag="VFU45_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU5357845.1"
                     /translation="MSIRQFRPMTAATRFKSVSGFDEITRATPEKSLLEPRKESGGRN
                     NLGHVTSRHRGGGHKQQYRRIDFKRDKFGVPGRVAEIEYDPNRSARIALVVYADGEKR
                     YILHPRGLAQGDTVVAGPGSDVRTGNALPLGEMPLGTSVHNVELKIGKGGQMIRSAGA
                     SAQVVAKEGDYVTLRLRSSEMRRVHGRCLATIGEVGNAEHELLSVGKAGKTRWLGQRS
                     KVRGVAKNPVDHPLGGGEGKTSGGRPPVSPWGKPEGRKTRHRKKASTRHIVRGRKRGK
                     ATR"
     gene            complement(2177..2464)
                     /gene="rplW"
                     /locus_tag="VFU45_01910"
     CDS             complement(2177..2464)
                     /gene="rplW"
                     /locus_tag="VFU45_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU5357846.1"
                     /translation="MPATHEVIVRPVVTEKSSAAFQTRKEYAFEVHPLANKHQIRDAL
                     QQLFGVTVTDVRTMQMRRHAVTRGRTRGTTARWKKAIVTLKDGDSIAVFEG"
BASE COUNT          291 a          949 c          813 g          489 t
ORIGIN      
        1 gatccgcttc ggtgccagca tggtcctcag gcccccgtgc tgtaggtgcg gccgcccagg
       61 tcctcgacga cctcaccctt gaagatccag accttgacgc cgatggtgcc ggaggtcgtc
      121 ctggcggtgg acgtggcgta ctccacgtcc gcgcggagcg tgtggagcgg cacccggccc
      181 tcgcgatagc cctcggtgcg cgcgatctcg gccccgccca ggcggcccga cgtgcgcacc
      241 ttgattccct gcgcgcccat ccgcatggcc gactgcaccg cccgcttcat cgcgcggcgg
      301 aacgagatgc gctgctccag ctggtgcgcg atgttgtcgg ccaccagctg cgccgcgagc
      361 tccggccgct tgatctcctc gacgttgatc gccgcttcct tgccggtgag ctgcgccagc
      421 tcgtcacgga gcttgtccac ctcggcgccc cgcttgccga tcaccacccc cggacggccg
      481 gtatgcaccg tgaccgtcac cttgccgggc cgccgctcga tgtgcacctc ggcgatcgcc
      541 gcgtgcccga ggcgcgcctt caggtacttc cggatgagct cgtcctcctt gaggagggcc
      601 ggcatgtcct tgcccgcgaa ccagcgcgac tgccagggct tgatgatgcc gaggcggaag
      661 cccgtcggat gcgtcttctg tcccatcagt tggtctccct ggcggccacg tggatctcca
      721 cgtggctggt ccgcttccgg atcggcgtgg cgcggccctg ggccgcggcg gtgaagcgct
      781 tgagcggctg ccccatgtcc accaccgcct tgctcaccac catcacatcc acgtcgaacg
      841 gctcgttgtc cttcagggcc gcctgctccg cgttggccac cgccgaccgg agggtcttct
      901 cgatctgccg cgccgcgagc ttcttcgaga acttgagcag cgcgtaggcc tcgttgacgt
      961 ccttgccgcg gatgaggtcc atcacgagcc gcatcttccg ggggctctgg cgcacgcccc
     1021 gctgaatcgc ctgtccggtc atggtcactc cggcttcgcc ttcttgtcgg ccacgatctt
     1081 gccgctgtgg ccgcggaaga gcctggtcgg cgcgaactcg ccgagcttgt ggcccaccat
     1141 gttctcgggc acgtccaccg gcccgaactt gttgccgttg tgcaccgcga aggtgtgccc
     1201 cacgaactcg ggcaggatgg tgctcgcccg gctccaggtc ttgaccacct tcttctcgtt
     1261 ccggccgttg agcccgtcca ccttgcggcg gagcgcgtcc tgcacgaacg ggcccttctt
     1321 gacgcttctt gccatttatc gggtcgcctt tccgcgcttc cgtccccgca cgatgtgcct
     1381 ggtggacgcc ttcttccggt gccgggtctt ccggccctcc ggcttgcccc acggggatac
     1441 cggcggccgg ccgcccgagg tcttgccctc gccgccgccc agcgggtggt ccaccgggtt
     1501 cttggccacg ccacggacct tcgagcgctg gccgagccag cgggtcttcc ccgccttgcc
     1561 gaccgacagc agctcgtgct cggcgttgcc gacctcgccg atcgtggcca ggcagcgccc
     1621 gtgcacccgc cgcatctcgc tcgaccgcag gcgcagggtc acgtagtcgc cctccttggc
     1681 caccacctgg gcgctggcgc cggccgagcg gatcatctgc ccgcccttgc cgatcttgag
     1741 ctccacgttg tggaccgagg tgccgagcgg catctcgccc agcgggagcg cattgccggt
     1801 gcggacgtcc gaaccgggcc cggccacgac cgtgtccccc tgggccaggc cccgggggtg
     1861 taggatgtag cgcttctcgc cgtcggcgta caccaccagg gcgatgcggg cgctccggtt
     1921 gggatcgtac tcgatctccg ccacccggcc cggcacgccg aacttgtcgc gcttgaagtc
     1981 gatgcgccgg tactgctgct tgtgcccgcc gccgcggtgc cgcgacgtga cgtgccccag
     2041 gttgttccgg ccgcccgatt ccttgcgcgg ctcgagcagg ctcttctccg gcgtggcacg
     2101 ggtgatctcg tcgaatcccg agaccgactt gaaccgggtc gccgccgtca tcgggcggaa
     2161 ctgtcggatg ctcatatcag ccctcgaaca cggcgatgga atcgccgtcc ttgagcgtga
     2221 cgatcgcctt cttccagcgt gccgtggtgc cgcgcgtgcg gccccgcgtc accgcgtggc
     2281 gccgcatctg catggtgcgg acgtcggtta cggtcacgcc gaacagctgc tgcagcgcgt
     2341 cacggatctg gtgcttgttg gccagcggat gcacctcgaa ggcgtattcc ttccgcgtct
     2401 ggaacgcggc ggagctcttc tcggtgacca ccgggcgcac gatcacctca tgcgtggccg
     2461 gcatctactt gcccgccttc ttcttcggcg cccgcctggc gctcttcgcc ttgcccttgg
     2521 cgggtgccgc cttggtcttc tt
//
LOCUS       DASUAP010000077         4939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_778712, whole genome shotgun sequence.
ACCESSION   DASUAP010000077 DASUAP010000000
VERSION     DASUAP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778712"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VFU45_01915"
     CDS             complement(<1..1169)
                     /locus_tag="VFU45_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5357847.1"
                     /translation="MNRRVRGVILASGLLMAGCGTPSLSGAPGAPPAPDRPWTPPARL
                     ARAEDSLGSVAIPPGLEARIHSLTLGDVIGIGLANNPVTRISWANARAAAAAYGAGRA
                     SWFPTIDGAINASHTRQPRPGASSVFEQDQLAPALNLSWLLWDFGGRTGNVAALRDAA
                     IAANFTHNSTLQGTVLLIEQAYFQYIATRALLAAQREALAEADTNLAAAEERHRVGLA
                     TIADVLQARTAASQAELVVETTEGDLQTTRGALATSLGFPANLPFDLDSTAAEVPVGR
                     LSDSVDALIDRGLQGRPDLQAARAQARAAEARYSLSRSALLPSLQATGTLGQTYSTSI
                     PAGASNYTVQVGLRIPLFDGFGRAQQAEAAAALADAARAQTDALRQQVVLDVFSA"
     gene            complement(1166..1642)
                     /locus_tag="VFU45_01920"
     CDS             complement(1166..1642)
                     /locus_tag="VFU45_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5357848.1"
                     /translation="MDRQSERFTERMAASLASDGYPRIAGRIYGLLLLSADGLSLDDL
                     TDRLGVSKASASTNARLLEQRGIVERFHLTSDRRDYYRVYVDHFERTMSQRLDKWQRV
                     SEALREARAAARDPVVMARLKMYEDRYGELSGAWREVLSRWAAGRQLASVETGAAR"
     gene            1791..3896
                     /locus_tag="VFU45_01925"
     CDS             1791..3896
                     /locus_tag="VFU45_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357849.1"
                     /translation="MTEPGPPPAASPATAAAETKLLSRELATFLVEFSIALHKSQIYP
                     AGHPLLDASVAGFTDRLHDLLEERTTLSLGVARHQLVIEGVATDPANPLLRELALKLH
                     RHHLGAVKFTQGLEYPEVAQFLAALAVDPHLSGDPLGRGEGDEIRRWPNIWLFPLAYG
                     QLELIEDDLPEGADPASRPEAAGRSTQLWVGLATAALAGQAGQEVPQPAAADPGEVAR
                     AIDEHARETTYDQVVVGYLLQIADELKMNPQPAGAGLQRRVSRLVSALRPETLRRLLE
                     MGGDATQRRRFVLDASQGMAVSAVVDLVKAAADTSQQNISHSLVRMLSKLAAHAEGGA
                     PEARKEADLALRDNVRQLLSGWDLENPNPDQYTGVLEAMSRNQATRGATMPDQECEPS
                     RVVEMAVEIDVLGESVRRAVDTMNERGQFADLLDLLERAPRTNDTVATLWEYIATPER
                     LRELLVRDEIPFELVDRLALRIGAPAAEPMLEVYAAAESRTVRRKLHDRLAQLGPGIG
                     PQVVERLSGTPWYVQRNMLALLGTMAAWPEGFSPEPFVRHEDARVRREAYRLLFRLPG
                     QRDAAVTAALADPDDAVLRLGLTAALEGLPAAAQPRVTAVATDAARPPEVRALAIRAL
                     GAVRSPATLPFLLEHATARQGLFRRDRLAPKSPEMLAALTALATSWAAHADAAPALEL
                     AARSEDPDVRAAVRRKGRR"
     gene            3893..>4939
                     /locus_tag="VFU45_01930"
     CDS             3893..>4939
                     /locus_tag="VFU45_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5357850.1"
                     /translation="MSAAAARFLHAMAQGLSTMALYRDGHPARERALDVAYEQLELLR
                     EGDPLPVFSFLAGEVIYRNEALREMKDWEWAGRLAQASIQRLEVTAQVDRAEFEEFLD
                     EVLARLALSAIDTATQRPGRLSGIRFGTIGVKGVTGDPRVELPATATINYTLAEEAET
                     VRWINQEVEERAALPLLEAEAVVRSLSVAMHGDSEIVIPLLQLKEFDQYTTTHSTNVS
                     VLTMALAEYLGLGPKDVRAYGVAGLLHDLGKVRIPKDILVKPGKLTEQERDIMRAHPT
                     EGARIILAREQRLDLAAAVAYEHHIVLNGGGYPSLHYQRDCHHGSKLVHICDFYDALR
                     TNRPYRGAWESDKVL"
BASE COUNT          711 a         1859 c         1698 g          671 t
ORIGIN      
        1 taggcgctga agacgtcgag gaccacctgc tggcggagcg cgtcggtctg ggcccgcgcg
       61 gcatcggcga gcgcggcggc ggcctccgcc tgctgggccc ggccgaatcc gtcgaagagc
      121 gggatcctga gccccacctg cacggtgtag ttgctggccc cggccgggat cgacgtgctg
      181 taggtctggc ccagcgttcc ggtggcctgc agcgatggga ggagcgccga gcggctgagc
      241 gaataccgcg cttccgcggc ccgtgcctgc gcccgcgcgg cctgcaggtc tggccgcccc
      301 tggaggccgc ggtcgatcag cgcgtccacg ctgtcggaga gccgccccac cggcacctcc
      361 gccgccgtcg aatcgaggtc gaacggcagg ttggccggga agccgagcga agtggccagg
      421 gcgccccggg tcgtctgcag gtctccttcg gtggtctcca ccacgagttc cgcctgggag
      481 gccgcggtcc gtgcctgcag cacgtcggcg atagtggcga gccccacgcg gtgccgctcc
      541 tccgccgccg cgaggttggt gtcggcctcc gccagcgcct cgcgctgcgc cgcgagcaac
      601 gcccgcgtcg cgatgtactg gaagtaggcc tgctcgatca gcaggacggt gccctgcagc
      661 gtcgagttgt gggtgaagtt ggccgcgatc gccgcgtccc ggagcgccgc cacgttcccg
      721 gtccgccccc cgaagtccca gaggagccaa ctcaggttga gcgccggggc cagctggtcc
      781 tgctcgaaca cgctcgaggc ccccgggcgc ggctggcgcg tgtgagacgc gttgatggcg
      841 ccgtcgatcg tggggaacca ggacgcgcgc cccgccccat acgccgccgc cgcggcacgc
      901 gcgttggccc acgagatcct ggtgaccgga ttgttcgcga gcccgatccc gatcacgtcg
      961 cccagcgtga gcgaatggat ccgcgcctcg aggccgggcg ggatcgccac gctgccgagc
     1021 gagtcctcgg cccgggccag gcgggctggc ggcgtccacg gccggtcggg tgcgggcggc
     1081 gcccccggcg caccggagag cgacggggta ccacaccctg ccatcaggag cccggacgcg
     1141 aggatgacac cgcggactcg acgattcatc gcgccgcccc ggtctcgacg gacgccagct
     1201 gccgcccggc ggcccatcgg gagagcacct cccgccacgc gccggagagc tcaccgtacc
     1261 ggtcctcgta catcttgagc cgcgccatca ccacggggtc gcgcgcggcg gcacgcgcct
     1321 cccggagcgc ctccgacacc cgctgccact tgtccagccg ctggctcatc gtgcgttcga
     1381 aatggtcgac atagacgcgg tagtaatcac gccggtcgga ggtgaggtgg aagcgctcca
     1441 cgatgccccg ctgctcgagg agccgggcgt tggtgctggc gctggccttg gagaccccca
     1501 gccggtcggt gaggtcgtcg agggaaagcc catcggcgct caacaggagc agcccgtaga
     1561 tccgcccggc gatgcggggg tagccatcgg aggcgaggct ggccgccatc cgttcggtga
     1621 atcgttcgct ctgccggtcc aagctcggtg ctcccgcggg aagggccatg tttcagtccg
     1681 ttcattacgg actgaatgcc gggaatgttg cagccccgcc cgggcggggc cagcacccgg
     1741 agggtgacct tgcgcaccga ccgggagtcc atccgactta cctttcagtg atgaccgaac
     1801 ccgggcctcc acccgcggca tcgccagcga ccgccgccgc cgagaccaag ctcctctcgc
     1861 gggagctggc gaccttcctg gtcgagttct cgatcgccct gcacaaaagc cagatctacc
     1921 ccgccggcca ccccctgctc gacgcgtcgg tggccgggtt caccgaccgc ctgcacgacc
     1981 tgctcgagga gcgcaccacg ctctccctcg gcgtcgcacg gcaccagctg gtcatcgaag
     2041 gtgtcgccac cgacccggcc aacccgctcc tgcgcgagct ggccctcaag ctgcaccgcc
     2101 accacctcgg cgcggtcaag ttcacccagg gactcgaata ccccgaggtc gcccagttcc
     2161 tggcggcgct cgcggtcgac ccccatctct ccggcgatcc cctcggccgg ggcgagggcg
     2221 acgagatcag gcgctggccc aacatctggc tcttcccgct cgcgtatggc cagctcgagc
     2281 tgatcgagga cgacctcccc gagggcgccg accccgccag ccgccccgag gccgccggac
     2341 gctcgaccca gctctgggtg gggctcgcca cggccgccct cgcgggccag gccggccagg
     2401 aggtgccgca acccgccgcc gccgaccccg gcgaggtggc cagggccatc gacgagcacg
     2461 cccgcgaaac gacctacgac caggtcgtcg tcgggtacct gctccagatc gccgacgaac
     2521 tcaagatgaa cccgcagccc gcgggtgccg ggctgcagcg ccgcgtgtcc cgcctggtct
     2581 cggcgcttcg gcccgagacg ctccgacggc tcctcgagat gggcggcgat gccacccagc
     2641 ggcggcggtt cgtcctcgac gcctcccagg ggatggcggt gagcgcggtg gtggacctgg
     2701 tcaaggccgc cgcggacacc tcccagcaga acatctctca ctcactggtg cggatgctgt
     2761 ccaagctggc ggcccacgcc gagggcggcg cgcccgaggc gcggaaggag gccgacctgg
     2821 ccctccgaga caacgtgcgc cagctcctgt cgggctggga tctcgagaac cccaaccccg
     2881 accagtacac cggcgtcctc gaggcgatgt cccggaacca ggccacgcgc ggcgccacga
     2941 tgcccgacca ggaatgcgag ccgagtcgcg tcgtcgagat ggcggtcgag atcgacgtcc
     3001 ttggcgagtc ggtccgacgc gccgtcgaca cgatgaacga gcggggccag ttcgccgacc
     3061 tgctcgatct cctcgagcgc gcaccccgca ccaacgacac cgtggccacc ctctgggagt
     3121 acatcgccac accggaacgg ctgcgcgagc tcctcgtccg cgatgagatc cccttcgagc
     3181 tggtggaccg cctggcgctc cggatcggcg cgcccgccgc cgagccgatg ctggaggtgt
     3241 acgccgcggc cgagtcgcgc acggtgcggc ggaagctgca cgaccgcctg gcccaactcg
     3301 gccccgggat cgggccgcag gtggtggagc gcctcagcgg cacgccgtgg tacgtccagc
     3361 ggaacatgct ggcgctcctc ggcacgatgg ccgcttggcc cgagggcttt tcccccgagc
     3421 cgttcgtccg gcacgaggac gcccgcgtgc ggcgcgaggc gtaccggctc ctcttccgcc
     3481 tgccgggcca gcgcgacgcc gccgtgaccg ctgccctcgc cgaccccgac gatgcggtgc
     3541 tccggctcgg gctcacggca gcgctcgaag ggctcccggc cgctgcccag ccccgcgtca
     3601 ccgcggtggc caccgacgcc gcccgccctc ccgaggtgcg cgcactcgcc atccgggccc
     3661 tcggcgccgt gcgctcgccg gccaccctgc cattcctgct cgagcacgcc accgcgcgac
     3721 aggggctctt ccgccgcgac cgcctcgccc cgaagtcacc cgagatgctc gccgcgctca
     3781 ccgcgctggc cacgtcgtgg gccgcccacg ccgatgccgc accggcactc gagttggcgg
     3841 ccaggagtga ggatcccgac gtgcgcgccg cggtgcggcg gaagggacgg cgatgagcgc
     3901 agccgcggcc cgcttcctcc acgcgatggc ccagggactg tcgacgatgg cgctctatcg
     3961 cgacggccat cccgcgcgcg agcgcgcgct cgacgtggcc tatgagcagc tcgagctcct
     4021 tcgcgagggc gatccgttgc ccgtcttctc ctttctcgcg ggcgaggtca tctaccggaa
     4081 cgaggcgctc cgggaaatga aggattggga atgggcggga cgcctggccc aggccagcat
     4141 ccagcgcctc gaggtcaccg cgcaggtgga tcgtgccgag ttcgaggagt tcctggacga
     4201 ggtgctggcg cggctggcgc tgtccgccat cgacacggcc acccagcgtc ccgggcgcct
     4261 gtccggcatc cgcttcggca ccatcggggt caagggcgtc accggcgatc cgcgggtcga
     4321 gttgcccgcc accgccacca tcaactacac cctcgccgaa gaggcggaga cggtccgctg
     4381 gatcaaccag gaagtggagg agcgcgccgc cctgccgttg ctcgaggccg aggccgtggt
     4441 ccggtcgctc tcggtggcga tgcacggtga cagcgagatc gtcatcccgc tcctgcagct
     4501 caaggagttc gaccagtaca ccaccacgca ctcgaccaac gtctccgtgc tcacgatggc
     4561 gctggcggag tacctggggc tcggccccaa ggacgtccgc gcgtacgggg tggccgggct
     4621 gctgcacgac ctcggcaagg tgcgcatccc caaggacatc ctggtcaagc ccggcaagct
     4681 caccgagcag gagcgcgaca tcatgcgcgc gcacccgacc gagggcgccc ggatcatcct
     4741 ggcgcgggaa cagcggctcg accttgcggc ggcggtggcg tacgagcacc acatcgtcct
     4801 caacggcggc gggtatccgt cgctccacta ccagcgcgac tgtcaccacg gcagcaagct
     4861 ggtgcacatc tgcgatttct acgatgcgct ccggaccaac cgcccctacc gcggggcctg
     4921 ggaatcggac aaggtgctg
//
LOCUS       DASUAP010000078         3353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_778803, whole genome shotgun sequence.
ACCESSION   DASUAP010000078 DASUAP010000000
VERSION     DASUAP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778803"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFU45_01935"
     CDS             complement(<1..234)
                     /locus_tag="VFU45_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5357851.1"
                     /translation="MANVPLLDLVAQYRAIQDQVLPALQSVIERQAFIMGAEVGQLEA
                     EVAKVCHAKHGVACASGTDAILIPLRALALRPGD"
     gene            complement(333..923)
                     /locus_tag="VFU45_01940"
     CDS             complement(333..923)
                     /locus_tag="VFU45_01940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU5357852.1"
                     /translation="MSDYFVHESSYVDDGAVIGKGSKIWHFSHVMAGAVIGERCNLGQ
                     NVVVMPGTRLGNNVKVQNNVSIYEGVILEDDTFCGPSCVFTNVMNPRSHVSRKHEYRT
                     TRVRRGASIGANATIVCGVTLGEYCFIGAGAVVNRDVPAYALMVGVPARRAGWMCQCG
                     ERLALHDGAAACGACGATYRESDGALRQIAPPSGRG"
     gene            complement(982..>3353)
                     /gene="carB"
                     /locus_tag="VFU45_01945"
     CDS             complement(982..>3353)
                     /gene="carB"
                     /locus_tag="VFU45_01945"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5357853.1"
                     /translation="IEMNPRVSRSSALASKATGFPIARIGTKVAVGYRLDELPNDITR
                     TTPASFEPVLDYVVVKIPRFAFEKFPAADPRLTTQMKSVGEVMAIGRTFKEALQKGLR
                     GLEIGRPGWTIGATPGDDRLQDGERETVRAAIRTPTPERVFQLKRALELGLEVAEIAE
                     RSGIDPWFLHQMRDLVEAERRWAAARPAGDEADRALLREMKRLGFSDRQLADLRGVPE
                     RDFRAERHRLGVRPVYKTVDTCAGEFPSSTPYLYSCYDVENEFEPPAGARTVIILGSG
                     PNRIGQGVEFDYCCVRAALAFRELGFRTVMVNSNPETVSTDYDISDTLYFEPLTLEDV
                     LEIVHRERPLGVVVQLGGQTPLRLARGLEAAGVAILGTSPDAIDAAEDRGRFEALARE
                     LGVAQPPSGTAMSVDDAVAVAERVGYPVLVRPSYVLGGRAMEIVYDADSLRRFFAIAA
                     RVAPGHPVLIDRFLEDAFEADVDAIADGTRCVIGGVMQHIEDAGVHSGDSACVLPPYL
                     LTEDQVEEMRRHTRSFALRLGVVGLLNVQYAVKDGVVYVLEVNPRASRTVPFVSKTCG
                     VPFAALAAAVMAGKTLDQLGLEDDVLQPYVAVKEAVFPFNKLHGVDLILGPEMRSTGE
                     VMGIAESFGMAFAKAQISADGALPLQGTVFITVNDHDKATATPIARRFHELGFRVLAT
                     EGTARYLRQRGIPADRVLKVHEGRPNAIDLLRSGEIQLLINTPLGKLTQQDDYAIRQA
                     ALQQRVSYTTTLSAASAACDAIIALRSRAGRVRSLQEWHQLAREGGAAG"
BASE COUNT          486 a         1240 c         1136 g          491 t
ORIGIN      
        1 gtcacccggc cgcagcgcga gcgcgcgcag cgggatgagg atcgcgtcgg tgccgctcgc
       61 gcacgccacc ccgtgcttgg cgtggcagac cttggccacc tcggcctcga gctgcccgac
      121 ctcggcaccc atgatgaacg cctgccgctc gatgaccgac tggagcgccg ggagcacctg
      181 atcctggatc gcgcgatact gcgcgaccag gtccagcaga ggaacgtttg ccacgatgga
      241 cccctgaacg aagttcgacg cgcccgcggt ccgatcggcc aatgccggcg agcccgggcg
      301 cgtggtagcg caagccgcta ctgggcctga atttagccgc gcccggaggg cggggcaatc
      361 tggcgcagtg ccccgtcgga ctcccggtag gtggccccgc aggcgccgca cgccgccgcc
      421 ccgtcatgga gcgccaggcg ctccccgcac tggcacatcc accccgcccg ccgcgccggg
      481 acccccacca tcagcgcata ggccgggacg tcccggttga ccaccgcgcc ggccccgatg
      541 aagcagtact cgccgagggt gacgccgcag acgatggtcg cattggcgcc gatcgacgcc
      601 ccccgccgga cccgcgtggt ccggtactcg tgcttccggg agacgtggct ccgcgggttc
      661 atcacgttgg tgaagacgca gctcggcccg cagaaggtgt cgtcctcgag gatcaccccc
      721 tcatagatcg acacgttgtt ctggaccttc acgttgttcc ccagccgggt gccgggcatc
      781 acgaccacgt tctgacccag gttgcagcgc tcgccgatca cggctcccgc catcacgtgg
      841 ctgaagtgcc agatcttgct ccccttgccg atgaccgcgc cgtcgtcgac gtaactgctc
      901 tcgtgcacga agtagtccga catgtccgct cgccgtcctt gttggatcgt tcgtcccctg
      961 ccccggtgac ctccggtccc gctacccggc cgccccgccc tcccgcgcca gctgatgcca
     1021 ttcctggagc gaccgcaccc tgcccgcccg gctgcggagc gcgatgatcg cgtcgcacgc
     1081 ggccgaggcc gccgacagcg tggtggtgta ggacacccgc tgctgcagcg ccgcctggcg
     1141 gatcgcgtag tcgtcctgct gggtcagctt cccgagcggc gtgttgatga ggagctggat
     1201 ctcgccggag cggagcaggt cgatggcgtt gggccgccct tcgtgcacct tgagcacccg
     1261 gtccgccggg atgccgcgtt gccgcaggta gcgcgccgtc ccctcggtgg ccaggacgcg
     1321 gaagccgagc tcgtggaacc gccgcgcgat gggggtggcg gtggccttgt cgtggtcgtt
     1381 gacggtgatg aacaccgtcc cctggagcgg gagcgccccg tcggcggaga tctgcgcctt
     1441 ggcgaacgcc atcccgaacg actcggcgat gcccatcacc tcgccggtcg agcgcatctc
     1501 gggcccgagg atcaggtcca ccccgtgcag cttgttgaag gggaacaccg cctccttcac
     1561 cgccacgtac ggctgcagca cgtcgtcctc gagtcccagc tggtcgaggg tcttccccgc
     1621 catcaccgcc gccgcgagcg cggcaaaggg caccccgcag gtcttcgaca cgaacgggac
     1681 cgtgcgcgag gcgcgcgggt tgacctcgag cacgtacacc acgccgtcct tgaccgcgta
     1741 ctgcacgttg aggagcccca ccaccccgag ccggagcgcg aaggaccggg tgtggcgccg
     1801 catctcctcc acctggtcct cggtcaggag gtaggggggc agcacgcagg cggagtcgcc
     1861 ggagtggacg ccggcgtcct cgatgtgctg catcaccccg ccgatgacgc accgcgtgcc
     1921 gtcggcgatg gcatcgacgt cggcctcgaa cgcatcttcc aggaaccggt cgatcagcac
     1981 cggatgcccc ggcgccacgc gggccgcaat ggcgaagaac cgccggagcg agtcggcgtc
     2041 gtagacgatc tccatcgccc gcccgcccag cacgtacgag ggacgcacca gcaccggata
     2101 ccccacgcgc tccgccaccg ccaccgcatc gtccaccgac atcgccgtgc cggacggggg
     2161 ctgggccacc ccgagctccc gtgccagcgc ctcgaagcgg ccgcgatcct cggcggcgtc
     2221 gatggcgtcg ggagaggtcc ccaggatcgc gacgccggcg gcctcgagac cgcgggcgag
     2281 ccggagcggc gtctgtccgc caagctgcac caccacgccc agcggccgct cccggtggac
     2341 gatctcgagc acgtcctcca gcgtgagcgg ctcgaagtag agtgtgtcgg agatgtcgta
     2401 gtcggtggag acggtctccg gattggagtt gaccatcact gtgcggaagc cgagttcgcg
     2461 gaaggccagc gccgcgcgga cgcagcagta gtcgaactcg accccctgtc cgatccggtt
     2521 ggggccgctc ccgaggatga tgaccgtgcg ggccccggcc gggggctcga actcgttctc
     2581 cacgtcatag cacgagtaga gatacggcgt cgagctcggg aactcgccgg cgcaggtgtc
     2641 gacggtcttg tacaccggcc gcaccccgag ccggtggcgc tcggcgcgga agtcccgctc
     2701 cggtacgccg cgcaggtcgg cgagctggcg gtcggagaag ccgaggcgct tcatctcgcg
     2761 caggagcgcc cggtcggcct cgtctcccgc cgggcgcgcc gccgcccagc gccgctcggc
     2821 ctcgaccagg tcccgcatct ggtggaggaa ccacgggtcg atgccgctgc gctcggcgat
     2881 ctcggccacc tcgagcccca gttcgagcgc gcgcttcagc tggaagaccc gctccggcgt
     2941 gggcgtccgg atcgcggcac ggaccgtctc ccgctccccg tcctgcagcc ggtcgtcgcc
     3001 cggcgtggcg ccgatcgtcc agccggggcg cccgatctcg agcccccgga gccccttctg
     3061 cagcgcctcc ttgaacgtgc ggccgatcgc catcacctcg cccaccgact tcatctgggt
     3121 ggtgagccgc ggatccgccg ccgggaactt ctcgaaggcg aagcgcggga tcttgaccac
     3181 gacatagtcg agcaccggct cgaacgacgc cggcgtggtg cgggtgatgt cgttggggag
     3241 ctcgtccagc cggtacccga cggccacctt ggtgccgatc cgggcgatcg ggaatcccgt
     3301 ggccttcgag gcgagcgccg acgaacgcga cacccgcggg ttcatctcga tga
//
LOCUS       DASUAP010000079         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1010560, whole genome shotgun sequence.
ACCESSION   DASUAP010000079 DASUAP010000000
VERSION     DASUAP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010560"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /gene="uvrC"
                     /locus_tag="VFU45_01950"
     CDS             <1..1723
                     /gene="uvrC"
                     /locus_tag="VFU45_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU5357854.1"
                     /translation="IYVGKARRLRSRVRSYVAAPDPADPPKHRMLVRAIADLETIVTP
                     NEAESLLLENNLIKEYQPRFNVRLKDDKTYPSIAVTIKDPFPRVLVTRRRDIPGAKYY
                     GPFTDVALMRRTLALVRRIFTVRSCPDDLPRERRERPCLDYHIGRCRAPCVGWQSQED
                     YRAMIGEVVAFLDGDTVDVRGRVRDLMLAASEREDFERARELRDTLKWLEQVDTPTTT
                     ETVGTGDADAIGYARDGDDAVVTFFRIRDGRVVARDHRFLENVEETPDPDILGAYLVQ
                     YYRLAEGRAGRLILPFPPADAPLVAQLLPESAWAVPQRGTAHRWLELADANARHLLES
                     LRIESFETDERAEDPVYALGRDLGLNTVPRTFICVDISTNQGRDTVGSLVWFEAGRPR
                     KAEYRKFKIKGPGQQDDFAAIHEVITRYLTRRRDESKPIPDLIVIDGGRGQLSSARDA
                     ASALGFGSVPMVSLAKREEEVYFPDRAEPLRLPRRSPSLRLLQRARDEAHRFGVTYNR
                     KRRTARTITSELLSIPGVGASRRRVLLEKFGSLAGVRAASVAELASVPGISTTMAERI
                     LARLAAR"
     gene            1765..2910
                     /locus_tag="VFU45_01955"
     CDS             1765..2910
                     /locus_tag="VFU45_01955"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU5357855.1"
                     /translation="MDWTLSCAACGATDLPDGLKTVCQCGQPWLVRYPDRAWSRATRD
                     ALGPGPGMWRYRTFLPLTASEAPVTLGEGMTPLLAIRRAGAELGATRLWLKDEGVNPT
                     GSFKARGLAAAITRASLAGAARFTLPTAGNAGVAASAYAARAGLPVRVYAPDSTPPTI
                     LSQIRWFGGDLVLLPGHIGDCGKASRQFAAEHNATDLSTLREPYRIEGKKTLGLEIAE
                     QFDWTLPDVILYPTGGGTGLIGMWKAFRELIDAGWVAGPAPRLYSVQSDGCAPVVRAF
                     AAGRASAEPWADPWTVASGLRVPGPLGDRLMLTALRETQGGAIAVSDSELTEAAARYS
                     RLEGLDLSPEGGATFAALRHLLASGQVSPDERIVLFNTGAGWLYRGA"
     gene            2947..>3512
                     /gene="larC"
                     /locus_tag="VFU45_01960"
     CDS             2947..>3512
                     /gene="larC"
                     /locus_tag="VFU45_01960"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HEU5357856.1"
                     /translation="MTPARIAIIDPAAGISGDMLLGALVDAGAPAAWLQGLPDRLGIG
                     EQVTVSIERVMRCGISCAKVNVRLADGSMEHPSDAVVAPHTHDHAAHGTGPHRHDPHA
                     GLHGHRHVADLVAIVERADLSAAVRARALQAFRSLGEAEGKLHGVPWDQVHLHEVGAM
                     DALVDIVGGIEGFERLGVQKVYARPVALG"
BASE COUNT          503 a         1233 c         1253 g          523 t
ORIGIN      
        1 catctacgtc ggcaaggccc ggcgactcag gtcacgggtg cggagctacg tggccgcgcc
       61 cgatccggcg gacccgccca agcaccgcat gctggtgcgc gccatcgccg acctcgagac
      121 catcgtcacg cccaacgagg ccgaatcgct gctcctcgag aacaacctca tcaaggaata
      181 ccagcctcgc ttcaacgtcc gcctcaagga cgacaagacc tacccgtcga tcgccgtcac
      241 catcaaggac ccgttcccgc gagtgctcgt cacccgccgg cgggacatcc ccggtgcgaa
      301 gtattacggg cccttcaccg acgtggcgct gatgcgccgg acgctggcac tggtgcgacg
      361 gatcttcacc gtccgatcct gtccggacga cctgccgcgc gaacggcgcg agcgtccctg
      421 tctcgactac cacatcggcc gctgcagggc gccctgcgtc gggtggcagt cgcaggagga
      481 ctatcgtgcg atgatcggcg aggtggtggc gttcctcgac ggggacaccg tcgacgttcg
      541 gggtcgcgtc cgcgacctga tgctggcggc gagcgagcgg gaggacttcg agcgcgcccg
      601 ggagctccgg gacacgctca agtggctgga gcaggtcgac accccgacca ccacggagac
      661 ggtgggcacc ggcgacgccg acgccatcgg atacgcccgc gacggcgatg acgcggtggt
      721 cacgttcttc cggatccggg acgggcgggt ggtggcgcgc gaccatcgct tccttgagaa
      781 cgtcgaggag accccggacc ccgatattct cggcgcatac ctggtccaat actaccggct
      841 ggcggaggga agggcggggc gcctcatcct gccgttcccg ccggccgatg cgccgctcgt
      901 ggcccagctc ctccccgagt ccgcctgggc cgtccctcag cgcggcacgg cgcatcgctg
      961 gctggagctc gcggacgcca atgcccggca cctgctcgag agcctccgga tcgagagctt
     1021 tgagaccgac gaacgggccg aggacccggt ctacgccctc gggcgtgacc ttgggctgaa
     1081 caccgtcccg cgcacgttca tctgcgtcga catttcgacc aaccaggggc gcgacacggt
     1141 gggctcgctg gtctggttcg aagcggggcg cccgcggaag gcggagtacc gcaagttcaa
     1201 gatcaagggc cccggccagc aggatgactt cgccgccatc cacgaggtca tcacccggta
     1261 cctgacccgc cgccgggacg agtcgaagcc gatccccgac ctcatcgtga tcgacggcgg
     1321 gcgcggccag ctgagctcgg cccgcgacgc ggcgtccgcc ctgggattcg ggtccgttcc
     1381 gatggtgagc ctggccaagc gggaggagga ggtgtacttc ccggaccggg ccgagccgct
     1441 ccgactgccg cgccggagtc cgtcgctccg gctgctccag cgggcgcgcg acgaggccca
     1501 ccgattcggc gtcacctaca accggaagcg ccggaccgcc cggaccatca cgtcggagct
     1561 cctgtcgatc cccggtgtcg gtgcgtcgcg gcggcgggtg ctgctggaaa agttcgggtc
     1621 gcttgccggg gtccgtgccg ccagcgtcgc ggagctggcg tcggtgcccg gcatctcgac
     1681 cacgatggcc gagcggatcc tggcacgcct ggcggcgcgc tgaccgccgg ggtgctgcca
     1741 cactgaacgg agcggtggtg cgatatggat tggacacttt cgtgcgctgc ctgcggtgcc
     1801 accgacctgc cggacggttt gaagacggtc tgtcagtgcg gccagccctg gctcgtccgc
     1861 tatccggacc gcgcgtggtc acgggccacc cgggacgcgc tcggcccagg gccggggatg
     1921 tggcgctatc ggaccttcct cccgctcacc gcatccgaag cgccggtgac cctcggcgaa
     1981 gggatgacgc cactgctcgc catccgccgc gccggagcgg aattgggcgc cacccggctc
     2041 tggctcaagg atgagggggt caacccgacc ggttcgttca aggcccgagg gctcgcggcc
     2101 gcgatcaccc gagcgtccct cgccggggcc gcccggttca ccttgcccac ggccggcaat
     2161 gccggggtgg cggcgtcggc ctacgccgcg cgggccggcc ttccggtgcg ggtgtacgcg
     2221 cccgattcga cccccccgac catcctgtcg cagatccggt ggttcggggg ggacctggtc
     2281 ctgcttcccg ggcacatcgg cgactgcggc aaggcctcgc ggcagttcgc tgccgagcac
     2341 aacgcgaccg acctctcgac cttgcgggaa ccgtatcgca tcgagggaaa gaagacgctc
     2401 ggcctcgaga tcgccgaaca gttcgactgg acgctgcccg acgtgatact gtacccgacc
     2461 ggcggcggta ccggcctgat cggcatgtgg aaggcctttc gcgagctcat cgatgccggc
     2521 tgggtggcag gaccggcgcc gcggctctat tcggtccagt cggacggctg cgcgccggtc
     2581 gtacgggcgt tcgcggccgg ccgcgccagc gccgagccgt gggccgatcc gtggaccgtg
     2641 gcgagcggat tgcgggtgcc gggcccgttg ggcgaccggc tgatgctgac cgcgctccgc
     2701 gagacgcagg gcggcgcgat cgccgtgagt gactccgagc tcacggaggc cgccgcgcgg
     2761 tactcgcgac tcgaagggct cgacctgtcg ccggaagggg gggcgacatt cgcggccctg
     2821 cggcacttgc tggcctcagg ccaggtgtcg cccgacgagc ggatcgtcct cttcaatacg
     2881 ggcgccggct ggctctatcg cggggcctga attgcaggcc accggtcgcc gggcgatatt
     2941 tccgtgatga cacctgcccg gatcgcgatc atcgaccccg ccgccggcat cagtggcgac
     3001 atgctgctgg gcgcgctggt cgacgccggc gcgccggcgg cgtggcttca ggggctgcct
     3061 gaccggttgg ggatcggcga acaggtgacc gtgtcgatcg agcgggtgat gcggtgcggg
     3121 atctcctgtg ccaaggtgaa cgttcgcctg gccgatggct cgatggagca tccgagcgat
     3181 gccgtcgtgg ccccgcatac ccacgaccac gctgcgcacg ggaccgggcc gcaccggcac
     3241 gatcctcatg ctggcctcca tgggcaccgg cacgtggcgg acctggtggc catcgtggag
     3301 cgggcggacc tgtccgccgc ggtccgggcc cgggccctgc aggcgttccg atccttgggc
     3361 gaggccgaag ggaagctcca cggcgtgccg tgggaccagg tgcacctcca cgaggtcggc
     3421 gcaatggacg cgctggtcga catcgtcggc gggatcgagg gcttcgaacg cctgggcgtc
     3481 cagaaggtct acgcccggcc ggtcgctctc gg
//
LOCUS       DASUAP010000080         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_70359, whole genome shotgun sequence.
ACCESSION   DASUAP010000080 DASUAP010000000
VERSION     DASUAP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70359"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFU45_01965"
     CDS             complement(<1..251)
                     /locus_tag="VFU45_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357857.1"
                     /translation="MPKRTDLKTILLIGSGPIVIGQGAEFDYSGTQAARALKEEGYRV
                     VLVNSNPATIMTDPEIADRTYIEPVTPEWVAKVIERERP"
     gene            complement(235..1173)
                     /locus_tag="VFU45_01970"
     CDS             complement(235..1173)
                     /locus_tag="VFU45_01970"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5357858.1"
                     /translation="MNVVVTGADGFVGQWLCRALLQAGHRVTGSHLAAAPAPGILSAE
                     ERATVRWRRLDLADDATVAALAAGPADQVVHLAGFASAGEAARDPGLAWVVNAAGTAR
                     LAHHLSLAAAPPLLLLVSSAEVYGAGEPRPRRETDPILPRSAYAASKAAAELAAEEVA
                     RRTGLRVVVARPFPHTGPGQTATYAFPAFAERIREAGRRGAATVPVGNLEPVRDLLDV
                     RDVVDAYLALLARGEPGEVYNVARGEGVQVGEVFDHLARILGVPVRPVADPALLRAAD
                     IPHLVGDPAKTFAATGWRPRRTLDETLQDLVDAQAD"
     gene            complement(1170..2132)
                     /gene="gmd"
                     /locus_tag="VFU45_01975"
     CDS             complement(1170..2132)
                     /gene="gmd"
                     /locus_tag="VFU45_01975"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU5357859.1"
                     /translation="MPKALITGITGQDGSYLAEFLLGKGYEVIGVVRRSSHDSYERIG
                     HLLERLTIVPADLLDQHSLTQVMQEHRPDEVYNLAAQSYVPTSWTQPVLTGEFTALGV
                     TRILEAIRLAHPGARFYQASSSEMFGKVVETPQTESTPFYPRSPYGVAKVYGHWITVN
                     YRESYDLFAVSGILFNHESPRRGMEFVTRKVTDGAARIKLGRATELRLGNLDAKRDWG
                     YAGDYVEAMWLMLQQKAPHDFVIGTGTQHTVRELVEIAFDRVGLDWKKHVVQDPRFMR
                     PAEVETLLADPSAARRELGWVPKTSFRELITMMVDADLERLARR"
     gene            2230..2421
                     /gene="rpmB"
                     /locus_tag="VFU45_01980"
     CDS             2230..2421
                     /gene="rpmB"
                     /locus_tag="VFU45_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU5357860.1"
                     /translation="MARVCSVCGKGPTTGNNVSHAKNRTKRRWYPNLQTVRVAQAGGV
                     RRIQVCTQCLKSNKVKKAV"
     gene            complement(2502..2858)
                     /gene="rplQ"
                     /locus_tag="VFU45_01985"
     CDS             complement(2502..2858)
                     /gene="rplQ"
                     /locus_tag="VFU45_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU5357861.1"
                     /translation="MRHNAKGRQLSRTSTHKRAMLNNMATSLFEHGRIVTTEAKAKEL
                     RPFAERLITLARRGDLHARRQVARKLKDPAVLQRLFAEIGPRFAARPGGYTRILKLGH
                     RPGDGADQARIELVEG"
     gene            complement(2858..3907)
                     /locus_tag="VFU45_01990"
     CDS             complement(2858..3907)
                     /locus_tag="VFU45_01990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU5357862.1"
                     /translation="MSIDLTGLVRPHFVEMTKRDDNPNVAEFRLQPLERGFGYTLGNS
                     LRRLLLSSLRGSAVWAFRLDGVVHEHQTIPGVLEDVHQIIQRLKNLVLVLSDDVDEAV
                     LKIKRDGKGPVYARDIAPNASVRVVNPDHLLFTLQDDRDVSAELYVNKGRGYVESELH
                     PADRSMPVDVVRIDAIYNPVRRANFTVAETRVGQRTDYDRLTVQVETNGTISPEDAVA
                     YAAALAQEHFRHFVEFGKVPQGRERGGQGADAELRDRLARPIDDFGLSVRSLNSLKNS
                     NIRTLGDLVTYSEDELLKVKNVGDKALGEIAELLRSQGLNFGMAFEESAGQLVVTNPG
                     AAPAARLTADGGEEA"
     gene            complement(3910..>4149)
                     /locus_tag="VFU45_01995"
     CDS             complement(3910..>4149)
                     /locus_tag="VFU45_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU5357863.1"
                     /translation="RRLDIPSYKVLVGQEIRVAPASRELTVVQASLERAARGQVPSWM
                     SVDTAKAVGRVLERPAREAIPINAQEQLIVELYSK"
BASE COUNT          585 a         1397 c         1542 g          625 t
ORIGIN      
        1 tcggggcgct cccgctcgat caccttggcc acccactccg gcgtgaccgg ctcgatgtac
       61 gtccggtcgg cgatctcggg gtcggtcatg atggtggcgg gattcgagtt caccagcacc
      121 acccggtagc cctcttcctt gagggcgcgc gccgcctggg tgccggagta atcgaattcc
      181 gcgccctgtc cgatcacgat ggggccggag ccgatgagga ggatggtctt caggtcagtc
      241 cgcttgggca tccaccaggt cctggagtgt ctcgtcgagg gtgcgccgcg ggcgccagcc
      301 ggtcgcggcg aaggtcttgg cggggtcgcc caccaggtgc gggatgtcgg cggcgcggag
      361 cagcgcggga tcggccaccg gccggaccgg gacaccgagg atgcgcgcca ggtggtcgaa
      421 tacctcgccg acctgaacgc cctccccccg ggccacgttg tacacctcgc ccggctcgcc
      481 gcgcgcgagc agcgcaagat aggcgtccac cacgtcccgc acatcaagga ggtcgcgcac
      541 cggctcgagg ttccccaccg gcacggtggc ggcgccgcgg cggcccgcct cgcggatccg
      601 ctcggcgaac gccgggaagg cgtaggtcgc ggtctggccg ggcccggtgt gcgggaaggg
      661 ccgcgccacc acgacccgga gcccggtacg ccgcgccact tcctccgccg cgagctcggc
      721 ggccgccttg ctggcggcat aggcggagcg cggcaggatc gggtcggtct cgcggcgggg
      781 ccgcggctcc ccggccccgt acacctcggc ggaggagacg agcaggagga gcggtggggc
      841 cgccgccagc gagaggtggt gggccagccg cgcggtgccg gcggcgttca ccacccaggc
      901 gagccccgga tcgcgcgcgg cctcccccgc cgaggcgaac ccggccaggt gcaccacctg
      961 gtccgcggga ccagcggcca gggccgcgac ggtcgcgtcg tccgcgaggt cgagccggcg
     1021 ccagcgcacg gtggcgcgct cctcggccga caggatcccc ggcgccggtg cggcggccag
     1081 gtggctcccg gtcacgcgat ggcccgcctg gagcagcgcc cggcagagcc actggccgac
     1141 gaacccgtcg gcgccggtga cgacgacgtt caccggcggg cgagccgctc gaggtcggcg
     1201 tcgaccatca tggtgatgag ctcccggaag ctggtcttgg ggacccagcc gagctcgcgc
     1261 cgcgcggccg acggatcggc caggagcgtc tccacctccg cggggcgcat gaaccggggg
     1321 tcctggacca cgtgcttctt ccagtcgagc cccacccggt cgaaggcgat ctcgaccagt
     1381 tcgcggacgg tgtgctgggt cccggtgccg atgacgaagt cgtgcggcgc cttctgctgc
     1441 agcatgagcc acatcgcctc gacgtagtcg ccggcgtagc cccagtcccg cttggcgtcg
     1501 aggttgccga ggcggagctc ggtggcccgg cccagcttga tgcgggcggc gccgtcggtg
     1561 accttgcggg tgacgaactc catcccgcgc cgcggcgact cgtggttgaa gaggatcccg
     1621 ctcacggcaa agaggtcgta cgactcccgg tagttgaccg tgatccagtg gccgtacacc
     1681 ttggccaccc cgtacgggga ccgggggtag aacggcgtcg actcggtctg gggcgtctcc
     1741 accaccttgc cgaacatctc cgacgaactc gcctggtaga accgcgcccc ggggtgggcg
     1801 aggcggatgg cctcgaggat ccgggtgacg ccgagggcgg tgaactcgcc ggtcagcacc
     1861 ggctgggtcc acgaggtcgg cacgtaggac tgggccgcga ggttgtacac ctcgtcggga
     1921 cggtgctcct gcatcacctg cgtgagggag tgctggtcga gcaggtccgc ggggacgatc
     1981 gtcagccgct cgaggaggtg accgatccgc tcgtagctgt cgtggctgga gcggcgcacc
     2041 acgccgatca cctcataccc ctttcccagc aggaattcgg cgaggtagga gccgtcctgg
     2101 ccggtgatcc cggtgatgag ggccttaggc atcggtcggt ccttccggcg gttgtgcccg
     2161 gtcgtccaag gggttatttt gcaaggcttt gacggacatg cagtcagtca ccattcgagg
     2221 gatcccacga tggcgcgggt gtgctcagtg tgcggcaagg ggccgacgac cggcaataac
     2281 gtcagccacg ccaagaaccg gacgaagcgg cgctggtacc cgaacctcca gacggtccgg
     2341 gtggcgcagg ccggcggcgt gcgccggatc caggtgtgca cccagtgcct gaagagcaac
     2401 aaggtcaaga aggccgtctg acgcccggaa gggcgagggg ctgaaaaaaa ggggacccgc
     2461 gggtcccctt ctttcgttcc tgggtgccgg ggccaatgcg ctcagccctc gaccagctcg
     2521 atacgggcct ggtcggcgcc gtcgccgggg cggtgcccca gcttgaggat gcgggtgtag
     2581 ccaccgggcc gggccgcgaa ccgcgggccg atctcggcga acagccgctg cagcaccgcc
     2641 ggatccttga gcttgcgcgc cacctggcgc cgggcgtgca ggtcgccccg ccgcgccagc
     2701 gtgatgagcc gctcggcaaa gggccggagt tccttggcct tggcctcggt cgtcacgatc
     2761 cggccgtgct cgaagagcga cgtggccatg ttgttgagca tggcccgctt gtgcgtgctg
     2821 gtgcgggaga gctggcgccc cttggcgttg tgccgcatca ggcctcctcg ccgccgtcgg
     2881 cggtgagccg ggcggccggg gccgcgcccg ggttggtgac gaccagctgg cccgccgatt
     2941 cctcgaaggc catgccgaag ttgagcccct gggagcgcag cagctcggcg atctcgccca
     3001 gcgccttgtc gcccacgttc ttgaccttga gcagctcgtc ctcggagtag gtcacgaggt
     3061 cgcccagggt gcggatgttg gagttcttga gcgagttgag cgagcgcacc gagaggccga
     3121 agtcgtcgat cgggcgggcc aggcggtcgc gcagttcggc gtcggcaccc tggccgccgc
     3181 gctcacggcc ctgcggcacc ttgccgaact cgacgaagtg gcggaagtgc tcctgggcca
     3241 gggccgcggc gtacgccacc gcgtcctccg gcgagatggt gccgttggtc tcgacctgga
     3301 ccgtgaggcg gtcgtagtca gtgcgctgac cgacgcgggt ctcggccacg gtgaagttgg
     3361 cgcgccggac cgggttgtag atcgcgtcga tgcggaccac gtccaccggc atcgaccggt
     3421 cggccgggtg cagctcggac tcgacgtacc cgcggccctt gttgacgtac agctcggccg
     3481 agacgtcgcg gtcatcctgc agggtgaaga gcaggtggtc gggattgacc acgcgtacgg
     3541 aggcgttcgg cgcgatgtcc cgggcgtaga ccgggccctt gccgtcgcgc ttgatcttga
     3601 ggacggcctc gtcgacgtcg tccgagagca ccagcaccag gttcttcagg cgctggatga
     3661 tctggtgcac gtcctcgagg acccccggga tggtctggtg ctcgtgcacc acgccatcga
     3721 ggcggaaggc ccagaccgcg ctcccccgca gggaggagag cagcaggcgg cggagcgaat
     3781 tgccgagggt gtacccgaag ccgcgttcga gcggctgcag gcggaactcg gcgacgttgg
     3841 ggttgtcgtc ccgcttcgtc atttcgacga aatgcggccg gacgagcccg gtcaggtcga
     3901 tgctcatggt cacttcgagt acagctcgac gatgagctgt tcctgggcgt tgatcgggat
     3961 ggcctcacgg gcggggcgtt ccagcacccg gccgacggcc ttggcggtat ccacggacat
     4021 ccagctgggg acctggccgc gggcggcgcg ctcgagcgac gcctgcacca ccgtcagctc
     4081 gcggctggcg ggcgcgacgc ggatctcctg gccgacgagc accttgtacg acgggatgtc
     4141 gagccggcg
//
LOCUS       DASUAP010000081         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_151989, whole genome shotgun sequence.
ACCESSION   DASUAP010000081 DASUAP010000000
VERSION     DASUAP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151989"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..650)
                     /locus_tag="VFU45_02000"
     CDS             complement(99..650)
                     /locus_tag="VFU45_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5357864.1"
                     /translation="MRPLRYSINVTLDGCCDHRVGIADEDLHRNASENIARADALLFG
                     RVTYQMMEEAWRTGAPAAWRQPWMEPFARTIDAARKYVVSSTLKSVDWNAELLRGDLA
                     TAVRALKDRPGKGLFTGGVTLPQALAELDLIDEYEVVVHPRIVGRGPTLFAGLPKPVD
                     LKLVDRVEFGSGAVALRYVPRRM"
     gene            complement(700..1434)
                     /locus_tag="VFU45_02005"
     CDS             complement(700..1434)
                     /locus_tag="VFU45_02005"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEU5357865.1"
                     /translation="MPSSTFLQVALNGDRVHPAVPRTPTAIAEAASAAVRAGAHSVHA
                     HAFDGTGRETLDGGACANVLRSIRRLCPDTPVSLTTSATIVPDPAERLRIVAAWQDLP
                     DLVTANQGEPGIADLCDLLLRRGVEIEAGLLTVDDACAFVASGLASRCRRVLIEPLEL
                     DPEVAVRNAARMEEVVSAAGIALEQVHHGYGIACWDVNRRALARGHGIRTGLEDVTVL
                     PDGSPAPDNAALVTAAAALIRTHTGS"
     gene            complement(1646..2419)
                     /locus_tag="VFU45_02010"
     CDS             complement(1646..2419)
                     /locus_tag="VFU45_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEU5357866.1"
                     /translation="MTIAARRHLFVLAGGMLLGAALAAQSPAPAPGPAEIHTDASAQR
                     SVRPDLAVFTLTFTGIGKTPGEAGQAVAARADTLRRALQALGIPHDSLMIGSRWYWWR
                     GRVETIYGPSRYIPAPAGRLGPGTRVQDTLYRATDAIQVRTHDLTKVGPAIDAALALD
                     ITNISDIQFFATNTDAARTDALREATTSVRQQAEVIATADGARLGRTLSLSTGYDFGV
                     GGVVSGMSVRGGTSSTEIVQPLIPVTVTVTGRWELIDKQ"
     gene            complement(2823..3104)
                     /locus_tag="VFU45_02015"
     CDS             complement(2823..3104)
                     /locus_tag="VFU45_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357867.1"
                     /translation="MESPPRYRAPQGPPRCAQFGLREAGSVRLPAPGPPLQDFVRALR
                     VQAGAIVTPDTEFDPEATDSIRLNFSQNHQAAVQSVERIATMVDRYRQG"
     gene            3403..>3731
                     /locus_tag="VFU45_02020"
     CDS             3403..>3731
                     /locus_tag="VFU45_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5357868.1"
                     /translation="MKLQPLDCYCATLRQAARAVTVLYEEALAGSGLRATQYTALQVL
                     ARAPGLTITEIAQAIGIDQTTATRTLALVRKAGLAKDRVGDDRRERRWALTPKGEARL
                     QALEPRW"
BASE COUNT          572 a         1278 c         1333 g          548 t
ORIGIN      
        1 gaatcccatt gcgctcgacg aacctgctgt cgaccttcgc gctgatagct gatagctgat
       61 agctgatagc tgatagctga tagctgatag ctgatagctc acatccgtct cggaacatac
      121 cgcaacgcca ccgcgcccga cccgaactcc acccggtcga cgagcttgag gtccacgggc
      181 ttcgggagcc ccgcaaagag cgtcggtccg cggcccacga tccgaggatg caccacgacc
      241 tcgtattcgt caatgaggtc cagttccgcg agggcctgcg ggagcgtcac cccgccggtg
      301 aagagcccct tgcccggccg atctttgagg gcccgcacgg cggtggccag gtctccacgc
      361 aggagctcgg cgttccagtc gacgctcttg agcgtgctgg agacgacgta cttccgggca
      421 gcgtcgatcg tccgggcaaa aggctccatc cacggctgcc gccacgccgc cggggccccg
      481 gtccgccacg cctcttccat catctggtag gtcacccggc cgaagaggag cgcatcggcc
      541 cgcgcgatgt tctcagacgc gttccggtgc aggtcctcat ccgcgatccc cacccgatga
      601 tcgcagcagc cgtcgagggt gacgttgatg gagtagcgga gagggcgcac gcggtgttcc
      661 cgggtcaaag tacggttcgt gggcggcgtg atgtgccggt caggacccag tatgcgtgcg
      721 gatcagcgct gccgccgccg tgaccagggc ggcgttgtcc ggggctggcg acccatcggg
      781 caggacggtg acgtcctcga gcccggtccg gatcccgtgc cctcgtgcca gcgcgcggcg
      841 gttgacgtcc cagcaggcga tgccgtagcc gtggtgcacc tgttcgaggg cgatgcccgc
      901 ggcggacacg acctcctcca tccgggccgc gttccggacg gcgacctcgg ggtcaagttc
      961 gaggggctcg atcagcacgc gacgacagcg gctggccagc cctgaggcca cgaaggcaca
     1021 ggcgtcgtcg accgtgagca acccggcctc gatctccacc ccgcggcgga ggaggaggtc
     1081 acacaggtcc gcaatgccag gctctccctg gttggcggtc acgaggtcgg ggaggtcctg
     1141 ccacgccgcc acgatccgga gtcgctccgc gggatccggg acgatcgtcg ctgaggtggt
     1201 cagcgacacg ggcgtatccg ggcagagccg ccggatgctc cgaaggacat tcgcgcaggc
     1261 gccgccatcc agcgtctccc gccccgtccc gtcgaaggcg tgggcgtgca ccgaatgggc
     1321 accggcccgg acggccgcac tggccgcttc cgcgatggcg gtcggggtgc gcggcaccgc
     1381 ggggtggacc cgatcgccgt tgagcgcgac ctgcaggaac gtggatgacg gcaacgcgtg
     1441 ggtccggtgt cgagttggat gccgatcggg cccgccccgg ggaccgatgg cgcataccgc
     1501 caagcccttc agggcgcatt gccgagaggc gccttgaggc gccgagaagc gggggagacc
     1561 ggcatcgact gacccagggg tctctcccgc cggaaggggg acggcgtcca gttcccagcc
     1621 cgcgcgcccg ccggtagcgc cacgctcact gcttgtcgat cagctcccag cggccggtga
     1681 cggtgacggt gaccggtatg agcggctgga cgatttcggt ggagctggtc ccgccccgga
     1741 cactcatgcc ggatacgacc ccaccgacgc cgaaatcata cccggtgctc aatgacaatg
     1801 tgcggccgag gcgcgcgccg tccgccgtgg cgatcacttc ggcctgctgc cggacggacg
     1861 tcgtggcctc gcgcagggcg tcggtgcgtg ccgcgtcggt attcgtcgcg aagaactgga
     1921 tgtcggatat gttggtgatg tcgagtgcga gcgcggcgtc gatcgccggg cccaccttgg
     1981 tgaggtcgtg cgtgcgcacc tggatggcat ccgtcgcacg atacagcgtg tcctggaccc
     2041 gggtcccggg gccgagacgg ccggcggggg ctggtatgta gcgcgaaggt ccatagatgg
     2101 tctcgacccg accgcgccac cagtaccagc ggctgccgat catcagggaa tcgtggggga
     2161 tccccagtgc ctgcagggca cgccgaaggg tgtcggcgcg ggccgccacc gcctggccgg
     2221 cctcgcccgg cgtcttgccg atgccggtga aggtgagggt gaagacggcc aggtcgggac
     2281 gcacgctccg ttgtgcgctg gcgtcggtat ggatctcggc gggtccaggc gccggcgccg
     2341 gcgattgggc ggcgagcgcc gcaccgagca gcatcccacc ggcgagcacg aacaggtgtc
     2401 gtcgagcggc gatcgtcatc gatcctcccg ggtgcgggtg gtgctccagg cgatggtcat
     2461 gcggcgccgc ccgaggcacc gctttcccta tttgacgacg gagggccccc gattgcgaca
     2521 atcagcaggt tcccgacgag gacaggcgga ctgcccggga cgggccggac cgtcaacgga
     2581 gccttcggca tgtacgggaa cagtccggca ccaacgagga ccccgcagag gacccggagc
     2641 catctaccgg cctccgggac ggccgctgcc gtggcggcca cgatgggcag ggcgcatccg
     2701 cacaccgccc ggccctgcgg ggcgcattgc cacggtacgg cgccggtcct gagcgcaacg
     2761 cttgccctga gcccgccgaa gggaaggggg tcggccgccg ggaagtgaac caccctcagt
     2821 cctcacccct gccgataccg gtctaccatc gttgcgatgc gctcgacgga ttggaccgca
     2881 gcctggtgat tctgcgagaa gttgagccgg atgctgtccg tagcctcggg atcgaactca
     2941 gtgtccggcg tgacgatggc gccggcctgc acccggagcg cgcgcacgaa gtcctggagc
     3001 ggcgggcccg gcgccgggag gcggacggag cccgcttccc tcaatccaaa ctgcgcgcag
     3061 cgcggcggtc cctggggggc gcggtaccgt ggggggctct ccatatggca gctcttccgc
     3121 gagcccgccg aggatccctt ccggcccgcg gatgtaccag aggcggcacg cgttctcgta
     3181 ctggaccacg ctcctgacga gtgtcgcgct gcatctaccg agccgggcga gcgggtcgct
     3241 gagctcctcg acgtcgagcg cgttgaccgg gccgcggcgg tgatcggcgg ccgcggtcga
     3301 tacgcacccg tcgaggatgc atcccggaac ggtgtccgtc aatgacgcga gtgccgtcgg
     3361 ccctcttgac agtgtatgca tatgcaccta ttgtcacctg ccatgaagct ccaacccctc
     3421 gactgctact gcgccacgct gcggcaggcc gcccgcgccg tcaccgtgct ctacgaggag
     3481 gcgctcgccg gcagcggcct ccgcgccacg cagtacacgg cgctgcaggt gctcgcgcgg
     3541 gcaccgggcc tgaccatcac cgagatcgcg caggccatcg gcatcgacca gaccacggcg
     3601 acgcgcacgc tggccctcgt ccggaaggcc gggctcgcga aggaccgcgt gggtgacgac
     3661 cggcgcgaac gccggtgggc gctgacgccg aagggggagg ccaggctgca ggccctcgag
     3721 ccgaggtggc a
//
LOCUS       DASUAP010000082         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_261050, whole genome shotgun sequence.
ACCESSION   DASUAP010000082 DASUAP010000000
VERSION     DASUAP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261050"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VFU45_02025"
     CDS             complement(<1..1107)
                     /locus_tag="VFU45_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5357869.1"
                     /translation="MSLASRALSRPVSTIAATIAVVLLGVISLDRLPVSLLPDVTLPV
                     LTIRTAYPGAAASEVSRLVAEPIEQSIAATPGLVDLRSVSRNGEVTTTARFAWGTDMA
                     ATVLTVRERLDNARGSLPETAERPTLLTSDPGERPIAVLALTGPGDLRSIARTATDVH
                     SRRLEQLDGVASVAVVGAPEDEIRIEVNPDRMRALGLSPDDIATAVENANRSVPGGTI
                     RRGQFRFSVRTFAEFRTPDEIGETPVGPPGAGIKLSDLATVTLTSADPRTETLLDGKP
                     AVGLVVYKDAGSNTVAVTQGIYKAMDQLKAEFPDVTMHVVAAQARFVTDALGNLTQEI
                     VAGGVLSLLVILFFLRDWRASLAIGLIVPLSVLMA"
     gene            complement(1154..>2267)
                     /locus_tag="VFU45_02030"
     CDS             complement(1154..>2267)
                     /locus_tag="VFU45_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5357870.1"
                     /translation="ESTMTLRAHLAVATLVLLPLSACKKSQADNGGADSTATRSASTL
                     SLPVVGDLVRKGDLVLTVTTNGQVRSDVAHLKSEAAGTIQEVLVRSGQHVTRGQPLIR
                     FDPRPFDLEVRAAEAAVAQAEVSYADNIVPDSIVSGQAPSEERRKNALARSGLEAARV
                     RLDQAKLARDRAVITAPFSGVVDEVSTAAGSRVGAGEELATVVSLSSMRVEANVLEHD
                     LPLIRQGGKAIVTTAAEPDKEIVGRIAAVLPLVDSTTRAGRVIVSLPNPGPLRPGMYA
                     DVRLESSRLPNEVIVPASAVLEREGRPLVFVVKGGKAKWTYVNPGRSNGAETIVLPDS
                     ASNEIPVAAGDTVLISGHLTLTHDAPVKVEMRKGSS"
BASE COUNT          322 a          749 c          872 g          324 t
ORIGIN      
        1 cgccatcagc accgagagcg gcacgatgag cccgatggcg agcgaggcgc gccagtcccg
       61 gaggaagaag agaatcacca gcagcgagag caccccgccg gccacgatct cctgcgtcag
      121 gttgccgagg gcgtcggtca cgaagcgggc ctgcgccgct accacgtgca tggtgacatc
      181 gggaaactcg gccttgagct ggtccatcgc cttgtagatc ccctgcgtca cggcgacggt
      241 gttggagccg gcgtccttgt acaccacgag cccgacggcg ggcttcccgt ccagcagcgt
      301 ctcggtgcgc ggatcggcac tggtgagggt gacggtggcg agatcggaga gtttgatgcc
      361 ggcgccgggc gggcccaccg gcgtctcgcc gatctcgtcc ggcgtccgga actccgcgaa
      421 ggtccgcacc gagaagcgga actgcccgcg gcggatggtg ccgcccggca cgctgcggtt
      481 ggcgttctcc accgcggtgg cgatgtcgtc gggggagagc cccagggcgc gcatccggtc
      541 ggggttcacc tcgatccgga tctcgtcttc gggcgcgccg accaccgcga cggaggccac
      601 cccgtcgagc tgttccagcc ggcggctgtg cacgtcggtg gcggtgcggg cgatcgagcg
      661 gagatcgccg ggaccggtga gcgcgaggac cgcgatgggt cgctcgcccg ggtcgctggt
      721 gaggagggtg ggccgttccg ccgtctccgg cagcgagccg cgcgcgttat cgagccgttc
      781 cctcacggtg agcaccgtgg ccgccatgtc ggtgccccag gcgaagcgcg cggtggtggt
      841 cacctcgccg ttgcgggaga cgctccggag gtccaccagt cccggtgtgg cggcgatgga
      901 ctgctcgatc ggctcggcta cgaggcgcga gacctcgctg gcggcggcgc cgggataggc
      961 ggtgcggatg gtgagcaccg ggagcgtgac atcgggcagc agcgacaccg gcaggcggtc
     1021 cagcgaaatg acgccgagca gcaccaccgc gatcgtcgcg gcaatggtgc tgacggggcg
     1081 ggagagcgcg cgggaggcta gggacaaggg agctgagagg ttgtcacccc gagcgaagcg
     1141 agggggccat gactcacgac gaccccttcc gcatttcaac cttgaccggc gcatcgtgcg
     1201 tcagcgtgag gtgccccgag atgagcaccg tgtcgccggc ggcgacggga atctcgttgc
     1261 tcgccgagtc gggcagcacg atcgtctcgg cgccgttgga gcggccggga ttcacgtagg
     1321 tccacttggc cttcccgccc ttcaccacga aaaccagcgg ccgcccctcg cgctccagca
     1381 ccgcgctcgc gggaacgatc acctcgttgg ggaggcggct gctctccagc cgcacgtcgg
     1441 cgtacatgcc gggccggagc ggcccggggt tggggagact cacgatcacg cggccggcgc
     1501 gcgtggtgct gtccaccagc ggcagcacgg cggcaatgcg gccgacgatc tccttgtccg
     1561 gctcggcggc ggtggtcacg atcgccttgc cgccctgcct gatgagcggc aggtcgtgtt
     1621 ccagcacatt cgcctccacg cgcatggagg agagactcac cacggtggcg agctcctcgc
     1681 cggcgccgac ccgggagccg gcggcggtgg agacctcgtc caccacgccg ctgaacgggg
     1741 cggtgatcac cgcgcggtcg cgggccagct tcgcctggtc cagccgcacg cgggcggcct
     1801 ccagcccgga gcgcgccagg gcgttcttgc gccgttcctc gctcggcgcc tggccggaga
     1861 cgatcgagtc ggggacgatg ttgtcggcgt agctcacttc cgcctgggcc acggcggcct
     1921 cggcggcgcg gacctcgagg tcgaaggggc gcgggtcgaa gcggatgagc ggctggccgc
     1981 gggtcacatg ctggccggag cggaccagca cctcctggat ggtgccggcg gcttcgctct
     2041 tgaggtgggc cacgtcggag cgcacctggc cgttggtggt gacggtgagg accaggtcgc
     2101 ccttccgcac gaggtcaccc actaccggga gcgagagggt ggaggcggaa cgggtggcgg
     2161 tcgagtcggc gccgccgttg tcggcctggg acttcttgca ggccgagagc gggagcagga
     2221 ccagggtggc gacggcgaga tgggcccgca gggtcatggt ggactcg
//
LOCUS       DASUAP010000083         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1296572, whole genome shotgun sequence.
ACCESSION   DASUAP010000083 DASUAP010000000
VERSION     DASUAP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296572"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1340
                     /gene="aroC"
                     /locus_tag="VFU45_02035"
     CDS             120..1340
                     /gene="aroC"
                     /locus_tag="VFU45_02035"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU5357871.1"
                     /translation="MGHLRFLTAGESHGKALTVIVEGLPAGLPVAAPQVDRDLARRMQ
                     GHGRGARMKIEQDRIEWLAGVRAGETLGSPVALLIHNRDWANWEDVMAAEGTPGEARR
                     RRVTRPRPGHADLAGVLKYDRADARDILERASARETVARVAAGALARRLLEEFGVEVG
                     SHVVSLGGVVVSRPDPLPAPLNAASDRSPVRVLDAAAEAEIIGRIDAAKRDGDTLGGE
                     VEVVARGLVLGLGSHVHWDRKLDGRLAAMLMSIPAVKGVEIGLGFEAARRPGSAVHDP
                     IAADRGRDAAGGFRRLSNNAGGLEGGMTTGEPLVARVAMKPISTLMAPLKTVDLGTGR
                     EAQAQSERSDVTAVPAMGVIAEALVCLVLADAMLEKFGGDSLTEMRRNVTGYLAGLGA
                     RWEAVDRSAAEVEG"
     gene            1340..1858
                     /locus_tag="VFU45_02040"
     CDS             1340..1858
                     /locus_tag="VFU45_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU5357872.1"
                     /translation="MAQHVLLVGLPGSGKTTVGRLVAGKLGAGFVDFDLVITRKMGMP
                     IARVFGEVGEVKFREIERKTAESAFAGPPSIIAPGGGWAAQPGQLEAAKASCFTIYIK
                     VLATTAAQRAGGDGTRPLLAGADPVVAMRELLKEREPLYLTAHAQVKGDNRTAEAVAL
                     DVVALAKQHAGW"
     gene            1980..>3113
                     /locus_tag="VFU45_02045"
     CDS             1980..>3113
                     /locus_tag="VFU45_02045"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEU5357873.1"
                     /translation="MTTPKTKSPSKRAKAAAAPEAAAAAAAPAKPKRARAKAAGGAKR
                     GGAALVIVESPTKAKTIGKYLGAGFDVKATVGHLRDLPTRELGVAVEDGFKPKYVTIK
                     GKTKTLAEIKKAAKGAREVFLATDPDREGEAIAWHVADQIGSAVPTRRVLFQEITKDA
                     VQEAMAHPLDIDEQKVNAQQARRILDRLVGYKASPILWKTIKTGLSAGRVQTVALRII
                     VEREREIRAFKPQEYWSIDALCAKDGQAFEAALKKIDGHDPQLHDQAAAERVVADLRA
                     LPFVVSKVEKRQRRKQPSAPFTTSTLQQEANKKLGFSTRRTMRAAQDLYEGIDVGGDG
                     PVGLITYMRTDSVRVSDQAIAAVREFIGQQYAKPYLPAQPNTYR"
BASE COUNT          468 a         1078 c         1169 g          398 t
ORIGIN      
        1 ccccgcggat cgtcgacgat gcggcggccg gcgcctcgca gtgagcggtc cggtcccacg
       61 ccttccagcg gtgccgagcc cgaccagcgc gtcgggctcg ctgttctagg gtgccgccga
      121 tgggacacct ccggttcctc accgcgggcg agtcgcacgg caaggcgctc acggtcatcg
      181 tcgagggcct cccggccggc ctcccggtgg cggcccccca ggtggaccgg gacctcgccc
      241 gccggatgca ggggcatggc cgcggcgccc ggatgaagat cgagcaggac cggatcgagt
      301 ggctggccgg cgtccgcgcc ggggagaccc ttggttcgcc ggtcgcgctg ctgatccaca
      361 accgcgactg ggccaactgg gaggacgtga tggccgccga ggggacgccg ggcgaggcgc
      421 gccgccgccg cgtcacccgc ccccggccgg gccacgccga cctggcgggt gtcctcaagt
      481 acgaccgcgc ggacgcccgc gacatcctcg agcgggcgag cgcccgggag accgtggcgc
      541 gggtcgcagc gggtgccctt gcccggcggc tcctcgagga gttcggggtg gaggtcggga
      601 gccacgtcgt ctcgctcggc ggggtggtcg tctcccggcc cgacccgctc ccggcgccgc
      661 tcaacgccgc ctccgaccgt tcgccggtcc gggtgctgga cgcggcggcg gaagcggaga
      721 tcatcggccg tatcgacgcc gccaagcgcg acggcgatac cctcggcggc gaggtcgaag
      781 tggtggcgcg agggctcgtc ctcgggctcg gcagccacgt ccactgggac cggaagctcg
      841 acggacggct ggcggcgatg ctgatgtcga tcccggcggt gaagggcgtg gagatcgggc
      901 tcggcttcga ggcggcccgc cgccccgggt ccgccgtgca cgacccgatc gccgccgacc
      961 ggggccgcga cgcggcggga ggcttccggc gtctgagcaa caatgccggc ggcctcgagg
     1021 gtggcatgac cacgggcgag ccgctcgtcg cgcgggtggc gatgaagccg atctcgaccc
     1081 tgatggcccc gctcaagacc gtggacctgg gcaccggccg cgaggcgcag gcgcagagcg
     1141 agcgttccga cgtcacggcc gtgcccgcga tgggcgtcat cgccgaggcg ctggtgtgcc
     1201 tggtgctggc ggacgcgatg ctcgagaaat tcggcggcga ctcgctcacc gagatgcgcc
     1261 gcaacgtgac cggctatctt gccggcctcg gcgcccggtg ggaggcggtc gaccggtcgg
     1321 ccgcggaagt ggaggggtga tggcacagca cgtcctgctc gtggggctcc cggggagcgg
     1381 caagaccacc gtgggccgcc tggtggccgg gaagctcggc gccggcttcg tcgacttcga
     1441 cctcgtgatc acccggaaga tggggatgcc catcgcccgg gtcttcggcg aggtcgggga
     1501 agtgaagttc cgggagatcg agcggaagac cgccgagtcg gcgttcgccg gcccgccgtc
     1561 gatcatcgcg cccgggggcg ggtgggcggc gcagcccggc cagcttgagg ccgccaaggc
     1621 cagctgcttc accatctata tcaaggtgct ggccaccacc gcggcccagc gcgccggcgg
     1681 cgacggcacc cgccccctcc tggccggcgc tgacccggtg gtggcgatgc gggagctgct
     1741 caaggagcgg gagccgttgt acctgaccgc gcacgcgcag gtgaagggcg acaaccgcac
     1801 cgccgaggct gttgccctcg acgtggtggc gctggccaaa cagcacgctg gctggtaagt
     1861 cgttgtctga caacaagttg ctggccaccg ccttgcgggg gtcaggggga tgtcactttc
     1921 ttgcggggaa ccggtggatg ttccggtgac cgtggactgg caatgtgagc cgtgaatcga
     1981 tgaccactcc caagacgaag tctccctcca aacgagccaa ggcggcggct gcgcccgagg
     2041 ctgcggctgc ggccgccgcc ccggccaagc ccaagcgcgc gcgcgccaag gccgccggcg
     2101 gcgcgaagcg gggcggggct gcgctcgtga tcgtggagtc tcccaccaag gccaagacga
     2161 tcggcaagta cctgggcgcc gggttcgacg tgaaggccac cgtcgggcac ctgcgcgacc
     2221 tgccgacccg cgagctcggc gtggcggtgg aggacgggtt caagccgaag tacgtgacca
     2281 tcaagggcaa gacgaagacc ctcgccgaga tcaagaaggc ggccaagggt gcgcgcgagg
     2341 tgttcctggc caccgacccc gaccgcgagg gggaggccat cgcctggcac gtggccgacc
     2401 agatcggctc cgcggtgccg acccggcggg tgctcttcca ggagatcacc aaggacgcgg
     2461 tccaggaggc gatggcccac ccgcttgaca tcgacgagca gaaggtcaac gcccagcagg
     2521 cccgccgcat cctcgaccgc ctggtgggct acaaggcgag ccccatcctc tggaagacca
     2581 tcaagaccgg gctctcggcc gggcgggtgc agacggtggc cctccgcatc atcgtggagc
     2641 gggagcgcga gatccgcgcc ttcaagccgc aggagtactg gagcatcgac gcgctgtgcg
     2701 ccaaggacgg ccaggcgttc gaggcggcgc tcaagaagat cgacggccac gacccgcagc
     2761 tccacgacca ggccgcggcg gagcgcgtcg tggccgacct ccgcgcgctg ccgttcgtgg
     2821 tcagcaaggt cgagaagcgg cagcgccgga agcagccgag cgcgcccttc acgacgagca
     2881 cgctgcagca ggaggccaac aagaagctcg gcttctccac ccgccggacg atgcgcgctg
     2941 cccaggacct gtacgagggc atcgacgtcg gcggtgacgg gccggtgggc ctcatcacct
     3001 acatgcggac cgactcggtg cgcgtgtcgg accaggccat cgcggcggtc cgggagttca
     3061 tcggtcagca gtacgccaag ccgtacctgc cggcgcagcc caatacttat cgg
//
LOCUS       DASUAP010000084         7940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_179261, whole genome shotgun sequence.
ACCESSION   DASUAP010000084 DASUAP010000000
VERSION     DASUAP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179261"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1445
                     /locus_tag="VFU45_02050"
     CDS             105..1445
                     /locus_tag="VFU45_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5357874.1"
                     /translation="MSTGRGGRWLAALGLHRPELRAWALYDWGFAAMVTTITTAVFPI
                     YFVKVAAAHTPGPAATARLAWVSSAALAVAAAISPVLGALADQAPIKKRGLAACMAVG
                     VAACAGMWWVERGDLTLASWLLGIVLVGGTASFVFYEALLPHLARPDEIDRVSTAGYA
                     LGYFGGGILLALNLAWILRPAFFGLPSGPGLTPSQATLPTRLAFVSVAVWWLSFSVVS
                     LRGMREPPITAPEASRREPVRSAFRQLAHTVRLLRRYPQAGLLLAAFLVYNDGIQTII
                     KMATAYGTEIGIGSSALIGSILLVQFVGVPCAFLFGALAGRIGSKRAILLGLGVYAVI
                     AVLGYFMRTGTDFLVLAILVGTVQGGTQALSRSLFATLIPPARSGEFFGLFSVFEKVA
                     GIFGPLFFAAAIWVTGSSRSAILSVILFFVVGAALLLRVDVATGRVHATAPADE"
     gene            complement(1495..2724)
                     /locus_tag="VFU45_02055"
     CDS             complement(1495..2724)
                     /locus_tag="VFU45_02055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5357875.1"
                     /translation="MAESRETVAGAARRMRPFAVLWEPPTGVLLDVSAGGEVLTARIR
                     LAMIALLFVIPLLGMIGGDPWTDTVVGFTIAAVAAMIGLLLLWGVSREVKKRWIPFAT
                     SAIDVSIVSAALLYFAISGQPAVAVNSKILYEVYFLAIAMTCLRYDMRVTALAGGLAM
                     VEYVGVVWWTGAHYDLHGPLVTHAVYGTFSWYTQVSREILLFIATMLGVANVGRARKL
                     HELSTSDSLTGLHNRGYFDERFREEVFRAKRHHRVLSVAMLDVDRFKGFNDTWGHSAG
                     DIALKEVARAIRKALRQTDVVARYGGEEIVIILPETPPESAVRKLEHIRRLVADMPVP
                     LARRTLPAGTVTISAGIASAPEDGDNVDQLLGRADTRLFAAKQMGRDRVVGPDQHSGV
                     RPSSAEFRAIVDPGPAA"
     gene            complement(2914..4551)
                     /locus_tag="VFU45_02060"
     CDS             complement(2914..4551)
                     /locus_tag="VFU45_02060"
                     /inference="COORDINATES: protein motif:HMM:NF045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HEU5357876.1"
                     /translation="MPKLIPLAHQAPLPLDRLLLHEAPSADAIEMDVLFVGAGPAGLA
                     GAIELAQLARKAGRSDLQIGVLEKASGLGEHNLSGAVVNPRAFRELFPGLGDADFPFR
                     QKVDREAVYYLTSGGALRIPTPPTMHNAGNFSASISEMTRWLGAKAEELGVNVFTGFP
                     VDALLVEGNAVRGVRTTPSGLDRDGSRRGDYQGPTDVVAKVTVLTDGTRSALGQAWRE
                     WQKVASPNPQIFALGVKEVWKVRRPLDRIIHTLGWPLPGDAFGGSWCYPQGPEQVSLG
                     LVVGLDYRDAALDVHVLLQRMKGHPLFAEILDGGELLEWGAKTIPEGGYYALPDRLSG
                     DGLLMAGDAVGFVDVPSLKGVHYAMQTGMYAARAAFGALDAGATAAALGAYDRMVRES
                     YVVADMHRTRNMRLAFKDGFLVGAAKAGLMTVTGGRIPGGRIAMHADAEAEKAVGAAA
                     PFVPDGVRTFTKLDAVFKSGNATRDTIPQHLVVGRDITPAVAEFYSHLCPAGVYELAD
                     GVLRVNAPNCVDCKATDVLGPRWLPREGGSGPKYRLM"
     gene            complement(4696..5685)
                     /locus_tag="VFU45_02065"
     CDS             complement(4696..5685)
                     /locus_tag="VFU45_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU5357877.1"
                     /translation="MANILAFAESRGGELRKVALEAVAAARQAADATGGGEVHAMLVG
                     APGIGTKAPQLAKVGADVVVVVEHPALANGNPEVAAATAAARIKAGGYRAAFFAASAQ
                     GKDLAPRVAAALGVSLASDLTSCALSGDALTGTHPTHTGKIITTIRLTGSPAIVTLRP
                     GAVRVTDQPRAGKVEAVAPAIDPANAKVVVTSVGSRGETKLDLGEAPVIVSGGRGLKE
                     AANFKLVEDLAAAFGNAAVGATRAVTDDGWRDPSTQIGQTGRLVSPDLYVAVGISGAV
                     QHLAGMRTSRVIVAINRDKDAPIFKLADYGIVGDLFEVVPVLTAAVRKARAGQ"
     gene            complement(5685..6437)
                     /locus_tag="VFU45_02070"
     CDS             complement(5685..6437)
                     /locus_tag="VFU45_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEU5357878.1"
                     /translation="MKIAVCIKRVPDMDVRFSIAPDGKSLNPGELKYDISDFDGYAVE
                     AALQFNEKQGPGEVVVISLGPDGAQETIRKALGMGADRGILLKCDAPPFDGAAIATAL
                     AAELKDGGYDLVLFGRAANDTGNQSVGPMTAHALGLPCVSAVSKLEIAGGKGSAERQL
                     EGAVESLEFPLPAVVTIDEGVARPRLPSLKGIMAAKKKPLEVKPAQLGSVRLTVEKIE
                     LPADRPPGRIIGEGPDAVPELVRLLQDEAKVL"
     gene            6670..7632
                     /locus_tag="VFU45_02075"
     CDS             6670..7632
                     /locus_tag="VFU45_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357879.1"
                     /translation="MPRKKLSTLTASLSLMALLGTTACGDTTTSPSRMQRAAITPLCA
                     TGCTDIDPYPDSAGVFLGSGITPSACTDGTDADADGLEDFCEDNLALAFAPELKYWDA
                     DEIGREPHLAVRPLDAGADSVRIAYLLSYYLDAGSAGGFLCSLFWVRPECEGHNGDSE
                     AIWLDVYYHVTTQHWVLSRAEYSAHDGANVYARGAGEYPTNLEYPTHPGAYPRSWVSE
                     GKHANYAHRSDCTGGGPWGLDTCADNNASARVSAGAYLNIGSNAHHLSTQDCMPSTNP
                     DYEYYGSGRLECYWTNQRFRGWIPTWVGGSDSDPYSPKLLGHGF"
BASE COUNT         1181 a         2807 c         2649 g         1303 t
ORIGIN      
        1 tcgcccttcg tgcacgtgcc gatcgggccc tcgctcgcca tcatcgtcgg cgtgctgggc
       61 ggcgccgtgg tcctgtcgct gcggcggccg gcaccggacg ccgggtgagc accggacgcg
      121 gcggtcgctg gctcgcggcg ctcggcctcc accggccgga gctccgcgcg tgggccctgt
      181 acgactgggg gttcgcggcg atggtcacca cgatcaccac cgcggtcttt cccatctatt
      241 tcgtcaaggt cgccgcggcg cacacgccgg gaccggcggc caccgcgcgg ctggcgtggg
      301 tgagcagcgc cgcgctggcc gtggcggcgg ccatctcccc ggtgctcggc gccctggccg
      361 atcaggcgcc catcaagaag cggggcctcg ccgcctgcat ggccgtcggc gtcgccgcct
      421 gcgccgggat gtggtgggtc gagcgcggcg acctgacgct cgcgtcgtgg ctcctcggca
      481 tcgtgctcgt cggcggcacg gcgagcttcg tgttctacga ggcgctcctc ccgcacctgg
      541 cccgccccga cgagatcgac cgggtgtcga ccgcgggcta cgcgctgggc tacttcggcg
      601 gcgggatcct gctggcgctc aacctggcct ggatcctccg gccggcgttc ttcggcctcc
      661 cgagcgggcc cggcctcacg ccgagccagg ccacgctccc gacccggctg gcgttcgtgt
      721 cggtggcggt gtggtggctc tcgttctcgg tggtctcgct ccgcgggatg cgggaacccc
      781 ccatcacggc acccgaggcg tcccgacggg agcccgtgcg ctccgccttc cgccagctgg
      841 cccacaccgt gcggctcctc cgccgctacc cgcaggccgg cctcctcctc gcggcattcc
      901 tggtctacaa cgacggcatc cagaccatca tcaagatggc cacggcgtac ggcaccgaga
      961 tcggcatcgg ctcgagcgcc ctcatcgggt cgatcctgct ggtgcagttc gtgggcgtcc
     1021 cgtgcgcatt cctgttcggg gcgctggccg gccggatcgg cagcaagcgc gccatcctgc
     1081 tggggctcgg ggtctatgcg gtgatcgcgg tgctggggta cttcatgcgg accggcaccg
     1141 acttcctggt gctggccatc ctcgtgggca ccgtccaggg cgggacccag gcgctctcgc
     1201 ggtcgctctt cgcgacgctc atcccgccgg cgcgctcggg ggagttcttc gggctgttca
     1261 gcgtgttcga gaaggtggcg gggatcttcg ggccgctctt cttcgcggcc gccatctggg
     1321 tcaccggttc gagccggagc gccatcctga gcgtcatcct cttcttcgtg gtcggggcgg
     1381 cgctcctcct ccgggtggac gtcgcgacgg ggcgggtgca cgccaccgcc cccgcggacg
     1441 aataaggggg cgccgcccgg cgccccctgc cccacagccc cgaccagacc gcgctcaggc
     1501 cgcgggcccc gggtccacga tggcgcggaa ctccgcgctg ctcggccgga cgccgctgtg
     1561 ctggtcgggg cccaccacgc ggtcccggcc catctgctta gccgcgaaga gccgggtgtc
     1621 ggcgcgcccc agcagctggt cgacgttgtc gccatcctcg ggcgcgctcg cgatcccggc
     1681 gctgatcgtg accgtcccgg cggggagcgt ccggcgggcc agcggcacgg gcatgtcggc
     1741 cacgaggcgg cggatgtgtt ccagcttccg gaccgccgac tcggggggcg tctcgggcag
     1801 gatgatcacg atctcctcac cgccgtaacg cgccaccacg tcggtctgcc gcagggcctt
     1861 ccggatggca cgggccacct ccttgagcgc gatgtccccg gcgctgtggc cccaggtgtc
     1921 gttgaacccc ttgaaccggt cgacgtcgag catcgccacc gagagcaccc ggtggtggcg
     1981 cttggcacgg aagacctcct cgcggaagcg ctcatcgaag tagccgcggt tgtggaggcc
     2041 ggtcagcgaa tcgctggtgg agagctcgtg cagcttgcgg gcccgcccca cgttggcgac
     2101 gcccagcatc gtggcgatga agaggaggat ctcgcggctg acctgcgtat accaggagaa
     2161 ggtgccgtac accgcgtggg tcaccagcgg accgtgcagg tcgtagtggg cgccggtcca
     2221 ccagaccacc cccacgtatt ccaccatcgc cagcccgccc gcgagcgccg tgacgcgcat
     2281 gtcatagcgg aggcaggtca tcgcgatcgc gaggaagtac acctcgtaga ggatcttgct
     2341 gttgaccgcc accgccggct ggcccgagat cgcgaaatac aggagcgccg cgctcacgat
     2401 gctcacgtcg atggcgctgg tggcgaacgg gatccaccgc ttcttgacct cacggctcac
     2461 gccccagagc agcagcagcc cgatcatggc ggccaccgct gcgatggtga acccgaccac
     2521 cgtgtcggtc caggggtcgc cgccgatcat cccgagcagc gggatgacga acagcagcgc
     2581 gatcatcgcc aggcggatcc gggccgtgag cacctccccg ccggcgctca cgtcgagcag
     2641 cacgccggta ggtggctccc acagcacggc gaacgggcgc atccggcgcg ccgccccggc
     2701 aacggtctcc ctggattcag ccatgttccg cttgttctaa tgaagggtcg cgcccgatat
     2761 cggcgctcgc tgggtgcctt aatctgcaag agcaccgtgc gaggcgcaac ggcaggaagg
     2821 ccggcaattg caggttctta gcacgatcgg tgcgtcctgg gatccttcgg acgctggcgc
     2881 gtcctcagga tgacttgggc cgtaaggccc aggtcacatc agccggtact tgggtccgct
     2941 gccaccctcc cgtggaagcc atcgcggacc gaggacgtcg gtcgccttgc agtcgacgca
     3001 gttgggggca ttgacccgca gtacgccgtc ggccagctcg tacacgcccg ccgggcagag
     3061 gtggctgtag aactccgcca ccgccggcgt gatgtcccgt ccgacgacca gatgttgcgg
     3121 gatggtgtcg cgggtggcgt tccccgactt gaagacggca tcgagcttgg tgaaggtccg
     3181 cacaccgtcg ggcacgaatg gcgccgcggc gccgaccgcc ttctccgcct ccgcgtcggc
     3241 gtgcatcgcg atgcgcccgc cgggaatgcg cccccccgtc acggtcatca accccgcctt
     3301 cgcagcgccg accaggaacc cgtccttgaa cgcgagccgc atgttccggg tgcggtgcat
     3361 gtcggccacg acgtaactct cccgcaccat ccggtcatac gccccgagcg ccgccgcggt
     3421 ggcccctgcg tccagcgcgc cgaacgcggc ccgcgcggcg tacatccccg tctgcatcgc
     3481 gtagtgcacg cccttgagcg atggcacgtc gacgaacccg acggcgtccc ccgccatcag
     3541 gagcccgtcc cccgaaagcc ggtcgggaag cgcgtagtac ccgccctcgg ggatcgtctt
     3601 ggcgccccat tcgagcagct caccgccgtc gaggatctcc gcaaagagcg ggtgcccctt
     3661 catccgttgc aggagcacgt gcacgtcgag cgcggcatcg cgatagtcga gcccaaccac
     3721 cagccccagc gacacctgct ccggcccctg cggatagcac caactgccgc cgaacgcgtc
     3781 gcctggcagg ggccagccga gcgtgtggat gatccggtcg aggggccgcc ggactttcca
     3841 cacctccttg acgccgagcg cgaagatctg cggattcggc gaggcgacct tctgccattc
     3901 ccgccacgcc tggccgagcg cgctccgcgt cccgtcggtg aggacggtga ccttggccac
     3961 gacgtcggtc ggcccctggt aatcccctcg cctgctgcca tcgcgatcga gccccgatgg
     4021 cgtggtccga acacccctga cggcattccc ttccaccagc agcgcgtcca ccgggaagcc
     4081 ggtgaacacg ttcacgccga gctcctcggc cttggcgccg agccagcggg tcatctcgct
     4141 gatggaggcg gagaagttgc cggcgttgtg catcgtggga ggcgtcggaa tccggagcgc
     4201 gccgcccgat gtcaggtaat agaccgcctc ccggtccacc ttctggcgga acgggaagtc
     4261 ggcgtcgccg agcccgggaa agagctcgcg gaaggcgcgc gggttgacca ccgcgccgga
     4321 gagattgtgt tcgcccaatc cgctcgcctt ctcgagcacg ccgatctgga ggtcggaccg
     4381 gcccgccttc cgcgccagct gtgccagttc gatggcgccc gccagccccg cgggcccggc
     4441 ccccacgaag agcacgtcca tctcgatcgc gtccgcgctc ggcgcctcgt ggaggagcag
     4501 acggtcgagg ggaaggggag cttggtgggc gagcgggatg agtttgggca cggggcttgg
     4561 ggtctgaggt ctcgggtctc gggtctcggg tctcgggtct cgggtctcgt gaagataacg
     4621 gccaacgacc aacgacgtgc gacattcgac ctgcgactcg tgacctgcga ctcgtgaccc
     4681 gcgacccgcg accccctact gccccgccct ggccttccgt accgccgcgg tcagcaccgg
     4741 caccacctcg aagaggtcgc ccacgatgcc gtagtcggcg agcttgaaga tcggggcgtc
     4801 cttgtcgcgg ttgatggcga cgatgacgcg cgaggtgcgc atcccagcca ggtgctggac
     4861 ggcgccggag atgccgacgg ccacgtagag gtcggggctc accagccggc cggtctggcc
     4921 gatctgggtc gacggatcgc gccagccatc gtcggtgacg gcccgggtgg cgccgacggc
     4981 cgcattgccg aacgcggcgg cgaggtcttc caccagcttg aagttcgcgg cctccttgag
     5041 cccgcgcccc ccgctcacga tcaccggcgc ctcacccagg tcgagcttcg tctcgccgcg
     5101 gctccccacc gacgtgacga cgaccttggc gttcgccgga tcgatggccg gggccaccgc
     5161 ctcgaccttg ccagcgcgcg gctggtcggt gacccggacg gcgccggggc ggagcgtcac
     5221 gatggccggc gatcccgtga gcctgatggt ggtgatgatc ttgccggtgt gcgtcgggtg
     5281 ggtgccggtg agcgcgtcgc cagagagcgc gcagctcgtg aggtcggagg cgaggctcac
     5341 gccgagcgcc gccgccacgc gaggagccag gtccttcccc tgggccgagg ccgcgaagaa
     5401 ggccgcacgg tagccgcccg ccttgatccg cgcggcggcc gtggcggcgg ccacttcagg
     5461 gttgccgttg gcgagcgccg gatgttccac caccaccacg acgtccgcgc ccaccttggc
     5521 gagctgcggc gccttggtgc cgatgccggg ggcgccgacg agcatcgcgt ggacctcgcc
     5581 accgccggtg gcatcggcgg cctggcgcgc cgccgccacc gcctcgagcg ccaccttccg
     5641 gagctcgccg ccccgcgatt ccgcgaacgc aaggatgttg gccatcagag caccttcgct
     5701 tcgtcctgga ggagcctgac cagctcgggc accgcgtcgg ggccttcccc aatgatccgt
     5761 cccggcggcc ggtcggcggg gagctcgatc ttctcgacgg tgagccgcac cgatccgagc
     5821 tgggccggct tcacctcgag cggcttcttc ttggccgcca tgatcccctt gagcgagggc
     5881 aacctcggcc gcgccacgcc ctcgtcgatc gtgaccaccg ccgggagcgg gaactcgagc
     5941 gactcgaccg ccccctcgag ctggcgttcg gcggagccct tgccgccggc gatctcgagc
     6001 ttcgacaccg cgctgacgca ggggagcccg agcgcgtgcg ccgtcatcgg gccgaccgac
     6061 tggttccctg tgtcgttggc tgcgcgcccg aagaggacca ggtcataccc gccgtccttg
     6121 agctccgcgg cgagcgccgt tgcaatggcg gcgccgtcga agggcggcgc gtcgcacttg
     6181 aggaggatgc cgcggtcggc acccatcccg agcgccttcc ggatcgtctc ctgggcgccg
     6241 tcggggccga gggagatgac gacgacctca cccgggccct gcttctcgtt gaactggagc
     6301 gccgcctcga ccgcgtagcc gtcgaagtcg ctgatgtcgt acttgagctc gcccgggttg
     6361 agggacttcc cgtcgggcgc gatggaaaag cggacgtcca tgtcggggac gcgcttgatg
     6421 cagaccgcga tcttcaaacg aacctccgga attcccagga acttggccgc ctcagcgccc
     6481 ccaaaatagc tccgcccact ggagggtgac aggggagcga ctcatggacg cctgtcgcaa
     6541 aagcggccca agttgacggg aaagtagcct atgccgcttc ctgaccgcag atagtcttga
     6601 cgagcaccgg tctattacga cgcgccgcaa ccccagtcca gctttccgct ctcacccctt
     6661 ggagcaccaa tgccgcgcaa gaaactctcc acactcaccg cttccctttc attgatggcg
     6721 ctactcggta ccactgcctg cggcgacacg acaaccagcc catcgcgcat gcagcgcgcc
     6781 gccatcacac cgttgtgcgc aacaggatgt acggacatcg atccgtaccc cgactccgct
     6841 ggtgtgtttc tcggcagcgg cattacaccc agcgcttgca ccgatgggac tgacgcggat
     6901 gcggacggcc tcgaagactt ctgcgaagac aacctcgccc ttgcatttgc ccccgaactc
     6961 aagtattggg acgccgacga aatcggtcgc gaaccgcacc ttgcggtccg gccgctggac
     7021 gctggcgcag attcggtccg tatcgcctac cttctttcgt attacctcga tgccgggagt
     7081 gccggcgggt ttctatgcag cctcttttgg gtgcgccccg aatgcgaggg acacaatgga
     7141 gattcggagg ccatctggct cgatgtgtat tatcacgtta cgactcagca ttgggtgctg
     7201 agtcgggcag aatactctgc gcacgatgga gcgaacgtgt atgcgcgcgg cgctggcgag
     7261 tatccaacca acctggaata cccaactcac ccgggagcat atccgcggtc gtgggtgtct
     7321 gagggtaagc atgcgaacta tgctcaccga tctgactgca ctggaggcgg tccctgggga
     7381 ctggatacat gcgcggacaa caacgcgtca gcacgcgtct cggcgggcgc gtatctcaac
     7441 attggcagca acgcgcatca cttgtccacg caggattgca tgccaagcac caatcctgac
     7501 tatgagtact acggcagcgg gagactggag tgctattgga ccaatcagcg attccgcgga
     7561 tggatcccga cttgggtggg cggcagcgac tcggacccat acagccccaa gttactcggc
     7621 cacggttttt gagaggaaac caatctatga acgccctcgg gttcaaaatc gcggcctgtt
     7681 gcttgctatt cggtacgcca caccttgcgg ctcaacaagc atcttcacgc atcagggtga
     7741 ttccgttggc ccgtgtattt gcggcatcca cacagcgtga tccactgctc tcagagagag
     7801 aattaaacct tcggcccatg tctccgccca acaggggcga gactggtgca attatcggcg
     7861 gaatcgctgg cgccgtagtc ggggccgtag tctttaacgg aatttgcact tcgtatgacc
     7921 atggctcgtg tctgggcacg
//
LOCUS       DASUAP010000085         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_274717, whole genome shotgun sequence.
ACCESSION   DASUAP010000085 DASUAP010000000
VERSION     DASUAP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274717"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VFU45_02080"
     CDS             complement(<1..1172)
                     /locus_tag="VFU45_02080"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEU5357880.1"
                     /translation="MTTSKGWTIIAAALILGLLADLLLRSTPWSVGLVLWLGAALAVP
                     FVLRPEGRAGFALPALGALAAAAMMVLRASDDLWVFNLAALLTALAVLLFRARSGVVG
                     EATPEEGLGALVEATVHCMFGPFLLAARDLDWSGERSLGRRILVGVLLASPVLLVLGA
                     LLAGAEPVLGAYLRAVLDLPRLFRHAFVWGVFAWITAGYLRALTLHPEGRWRPGPGPR
                     IGEVESLTVLGAIGLLFAAYLVIEVRTLAGGAELVRSVTGMTYARYARQGFFELVVAA
                     GITLAALLAVDWARGRGEGEPDPRVRGLAWALLAMLGLVIASAFARLALYVSYYGLSS
                     TRVYAAMAIAWAAAASAWFGITVLRGRRKRFVAGALTAAVLWLVAADVANVDALIVR"
     gene            complement(1175..1885)
                     /gene="uppS"
                     /locus_tag="VFU45_02085"
     CDS             complement(1175..1885)
                     /gene="uppS"
                     /locus_tag="VFU45_02085"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU5357881.1"
                     /translation="MPPPRHVAIVMDGNGRWAERRGMPRGAGHQAGADAVHRVVRAAR
                     ERGIAVLSLYAFSSDNWRRPRPEVAALLALFDHFLAAETATCVAQGIALTILGRRDRL
                     PEHLLRSVERAERATAGGRAMRLRIAIDHSGRDAILAASRAAMGTTPGRDRFSDLVAA
                     ADHDLHPAVPVDLYIRTGGERRLSDFLLWEAAYAELLFLDVLWPDFTAADLDSALQDF
                     HSRDRRFGGLPASLETPA"
BASE COUNT          357 a          801 c          702 g          238 t
ORIGIN      
        1 cggacgatca gtgcgtccac gttggccacg tccgccgcca cgagccagag caccgccgcc
       61 gtgagcgccc ccgccacgaa ccgcttccgg cggccccgca ggaccgtgat cccgaaccac
      121 gctgacgccg ccgccgccca ggcgatcgcc atcgcggcgt agacccgggt cgagctgagc
      181 ccgtagtacg acacgtagag cgccagccgc gcgaacgccg atgcgatgac gaggccgagc
      241 atcgcaagga gcgcccacgc gaggccgcgc acgcgcgggt caggctctcc ctcaccgcgc
      301 ccccgcgccc agtccactgc cagcagcgcc gcgagcgtga tccccgccgc gaccaccagt
      361 tcgaagaacc cctgccgcgc gtagcgtgcg tacgtcatcc ccgtcaccga ccggaccagc
      421 tccgccccgc ccgccagggt gcgcacctcg atgacgagat aggccgcgaa caggaggccg
      481 atggcgccga ggacggtgag cgactcgacc tccccgatcc ggggaccggg tcccggcctc
      541 caccgccctt ccgggtgcaa cgtcagcgcg cgcaggtagc ccgccgtgat ccaggcgaac
      601 accccccaga cgaacgcgtg ccggaagagc cgcggcaggt cgaggacggc gcgaaggtag
      661 gcgcccagga ccggctcggc gcccgccaac agggctccga ggacaaggag gaccgggctg
      721 gcgaggagta cacccaccag gatccgccga ccgagcgagc gctcgccgga ccagtcgagg
      781 tcgcgggccg caagcaggaa gggaccgaac atgcagtgga ccgtcgcttc gaccagcgca
      841 ccgagccctt cctctggcgt cgcctccccg accacaccgc tccgggcccg gaagagcagc
      901 accgcaagcg ccgtgaggag cgcggccagg ttgaacaccc agaggtcgtc cgaggcgcgg
      961 agcaccatca tcgccgccgc ggccagggcg ccgagcgcgg ggagcgcgaa cccggcgcgc
     1021 ccctccggcc ggagcacgaa cggcacggcg agcgcggcgc cgagccagag gacgagaccg
     1081 acggaccagg gggtgctgcg cagcagcaag tcggcgagga ggccgaggat gagcgccgcg
     1141 gcgatgatcg tccagccctt cgatgtggtc atggtcacgc gggtgtctcc agcgatgcgg
     1201 gaaggccgcc aaagcggcgg tcccgggagt ggaagtcctg cagggcggaa tcgaggtcgg
     1261 cggcggtgaa gtcgggccac agcacgtcga ggaacagcag ctcggcgtac gccgcctccc
     1321 agaggaggaa atcgctcagc cgacgctccc cgccggtccg gatgtagagg tcgaccggca
     1381 ctgccggatg gaggtcgtgg tccgcggccg ccaccaggtc ggaaaagcgg tcgcgcccgg
     1441 gcgtggtgcc catcgccgcc cgggacgccg cgaggatcgc gtcacgcccc gaatgatcga
     1501 tcgcgatcct gagccgcatc gcccggcccc cggcagtggc ccgctcggcg cgctcgaccg
     1561 agcgaagcag gtgctcgggc aggcgatccc ggcgccccag gatggtgagg gcgatccctt
     1621 gcgccacgca ggttgccgtc tccgccgcca ggaagtggtc gaagagcgcc aggagcgccg
     1681 ccacctcggg ccgcggtcgg cgccagttgt cagacgagaa ggcgtagagc gagagcactg
     1741 cgatcccccg ctcccgggcg gcccgcacca cccggtgcac cgcgtcagcg ccggcctggt
     1801 gcccggcccc gcggggcatc ccacggcgct cagcccagcg gccgttgccg tccatcacga
     1861 tggcgacgtg gcgtggcgga ggcaaatgac tttgcatcat aaagtttcgg atcccgaaaa
     1921 aacgcctctc ggcgcgacca ctgatctcac ccccacacgc ggcacctgcc ctgcacatcg
     1981 cacctcacgt ttctcatctc accgctcacc tcttacctct tacctcttac ctcttacctc
     2041 tcacctctca cctcttacct ccatgcccct cccttctcgc acactcggtc gctccggc
//
LOCUS       DASUAP010000086         6173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1201340, whole genome shotgun sequence.
ACCESSION   DASUAP010000086 DASUAP010000000
VERSION     DASUAP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201340"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1220)
                     /locus_tag="VFU45_02090"
     CDS             complement(3..1220)
                     /locus_tag="VFU45_02090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5357882.1"
                     /translation="MTRPFEQVDVLVAGAGPGGSAVATLLAGLGLRVLAVDGARFPRE
                     KACSEFMSPAAVAVLDRLGVLDDLDAHGAHPAGTSVITGRGGALAGRFAPSAGGRFRG
                     AGLSVARRVLDQRLVEAARAAGAEVREAVTVRDLLYDGGSVAGAVVAGADGTLHAIRA
                     RLTIGADGLRSIVARRLGPRRHGAPHRMAFVAHVAGVRDLGDLAEMHVGPDGYVGLNH
                     IGGGVANVALVVPQRAAAAARGDAAGFFHRQLARYPGVAGRVDQGGVVREVMVTGPFA
                     ARSSRVVADGALLLGDAAEFFDPFTGEGIYRALRGAELAATVAARAMDRGTSPTRQAL
                     RPYVLARRAEFAGHWAVEWIIGWMMHAPALFDRAVRRIESRGLADRLIGVTGDLLPAG
                     AVLSPAFLFRTLL"
     gene            complement(1217..2005)
                     /locus_tag="VFU45_02095"
     CDS             complement(1217..2005)
                     /locus_tag="VFU45_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HEU5357883.1"
                     /translation="MSLPLAAAIAALVATLAALARALSGRGAVAAAAVGTAVLWTAGW
                     AGAAALFAFFLSSSIVSRVLPDPAAAAGEAKGGRRDAGQVLANGGAAAIAALLAAGRP
                     SPALWAITSSLAAAAADTWATAVGATSRGLPHDILSGAAVPPGTSGGVTTRGTLGALL
                     GAFVVAAAAALVVHDARLLVAAVAIGAIGMLADSVAGATVQGRFHCPACDATTERAVH
                     RCGTRTRLVAGHAWLTNDGVNALATGAAALLGIGAWCWIGMPAR"
     gene            complement(2007..2249)
                     /locus_tag="VFU45_02100"
     CDS             complement(2007..2249)
                     /locus_tag="VFU45_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5357884.1"
                     /translation="MTSHELIDCSRMLDELQDVLRGEATPETAAEVQRHLEVCSPCLR
                     YVTFEERFRELLRSCAGQRIPQDVRERLLASLRRAD"
     gene            complement(2246..2803)
                     /locus_tag="VFU45_02105"
     CDS             complement(2246..2803)
                     /locus_tag="VFU45_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5357885.1"
                     /translation="MTEATAAYETEVLPHLDVLFRVARRMTGNAADAEDLVQETLLKA
                     LRGWEGFRPGSNARAWLLTILRNAFISGYRRRKREPVTMDIDAADRHSVIRDVAEADP
                     EATFFTKLVDETVLKAVEALPEEFREALVLSDLEGLAYAEVAQVLDVPVGTVKSRLFR
                     ARRLLQRSLRDHALEQGYLKGGAAT"
     gene            2981..3616
                     /locus_tag="VFU45_02110"
     CDS             2981..3616
                     /locus_tag="VFU45_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357886.1"
                     /translation="MPVFPATEIDAIVPLSLLEAMRNVDTPVEDGLEELAGEIVAKRL
                     GLSPTVAAQIARYDWAAGRGAPVSQEEALAVFRLVGRRPDADLVFADAGRRAARRAAR
                     EAGVAPRALLGIVPEAAARPLGLGLARRLARRVFAGELTLKDAEATAQVPAPLGIDDV
                     PEGGACGFYGAAFAELLRALAGFEGAMRHIRCRARGDDQCQWRASAVGGYQ"
     gene            3613..4797
                     /locus_tag="VFU45_02115"
     CDS             3613..4797
                     /locus_tag="VFU45_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5357887.1"
                     /translation="MSGISAIDRAQLAEGLAELGADGWLLFDFHGLNPVAGRVLALGG
                     LGSRRLFVLIPRTGAPTAVAHKIELQPMEGFPGTIVPYARWEELHAALGPLVKGKTLA
                     MEVSPRDAVPYLDRIPHGVIQLLEGLGAKIVSSDQLVTRFAARWSAAELADHLAAAEV
                     LADVAKEQLAWAVTQGGKGLTESALQRRTVAAMEARGLRLTTHPIVGFGPNAANPHYE
                     PHEGQDRTLAPDEVVLLDLWGGRGRVDTVFADQTWMGFSGRRVPSKVQQVWEVVRDAR
                     DAAVALVQERVAAGKPLAGYEVDRAARGVIERAGFGASFVHRTGHSIDRDLHGSGPHM
                     DDFETHDERRLIPGVGFSVEPGVYLVGEFGIRSEVNMFWGESGARVTPTAPQRDLILP
                     GA"
     gene            5145..>6173
                     /locus_tag="VFU45_02120"
     CDS             5145..>6173
                     /locus_tag="VFU45_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU5357888.1"
                     /translation="MIQRCVRAVARVAHLATLAAVALVLGAGALSAQATAKIEGRVRD
                     SSGQPIANAQVFIVGSAFSALTNAQGYYFINNVPVGTISMKAAFVGYQPVQVNSVQVL
                     GGQTMTQDFSLKASVVTVQALEITAEHPLVPRDAVTSKSRIDGQMAEALPVDRVSSLL
                     ALQPGVVASASGGTLSIRGGRPTEAVTYVDGVPVTPGYRGGGFVGSGGSSITVNANGL
                     QEANVTSGATSAEFGNAQSGIFDFNTRSGGQKLSANLGVSTDQPFARTSSLGYTQIRG
                     GVGGPIPGVANLTYYVSALLEGQQSAATGFDANKVPVFVPAGIDKTVAVPSAYGDPFA
                     DTSYVPVEK"
BASE COUNT          848 a         2159 c         2323 g          843 t
ORIGIN      
        1 gctcacagca acgtccggaa gaggaaggcc ggcgagagca cggcgcccgc gggcaggagg
       61 tcgccggtga cgccgatcag gcggtcggcg aggccgcggc tctcgatccg ccgcacggcc
      121 cggtcgaaga gcgccggtgc atgcatcatc cagccgatga tccattcgac ggcccagtgc
      181 cccgcgaatt cggcgcgtcg cgccagcaca tacgggcgga gcgcctggcg tgtcgggctc
      241 gtgccgcgat ccatcgcccg ggccgcgacg gtcgccgcca gctccgcgcc gcggagcgcg
      301 cgatagatcc cctcgccggt gaacgggtcg aagaactctg cggcgtcgcc gaggaggagc
      361 gcgccatcgg ccaccacgcg ggaggaccgc gccgcgaagg ggccggtcac catcacctcg
      421 cggacaacgc cgccctggtc cacccggccg gccacgcccg gataccgtgc cagctgccgg
      481 tggaagaacc cggccgcatc gccgcgggcc gcggccgcag cacgctgcgg gaccacgagc
      541 gccacattcg cgacgccgcc accgatgtga ttgaggccga cgtatccgtc ggggccgacg
      601 tgcatctcgg ccaggtcgcc gaggtcgcgg acgccggcga catgcgccac gaatgccatc
      661 cggtgcgggg caccgtgccg ccgcgggccc aggcgccgtg ccacgatgga gcggagtccg
      721 tcagcgccga tcgtgaggcg ggcacggatg gcgtggagag tgccgtcggc gcccgccacc
      781 actgcgccgg ccacggagcc tccatcgtag agcaggtcgc gcacggtgac cgcttcgcgg
      841 acctccgcgc cggcggcgcg ggccgcttcg accagtcgtt ggtcgaggac gcgccgggcc
      901 acggagagcc cggccccccg gaagcgcccg cccgcgcttg gcgcaaagcg gcccgccagc
      961 gcgccaccgc gcccggtgat gaccgaggtc ccggcggggt gcgcgccgtg ggcgtcaagg
     1021 tcatcgagta cgcccaaccg gtccagtacc gcgaccgccg ccgggctcat gaactccgag
     1081 caggccttct cgcgcgggaa tcgggcgccg tcgaccgcga gtacgcggag ccccaggccg
     1141 gcgaggagcg tggcgacggc cgagcctccc gggccggccc cggccacgag gacatcgacc
     1201 tgctcgaagg gtcgggtcac cgggccggca tcccgatcca gcaccacgcg ccgatgccca
     1261 gaagggcggc ggcgccggtc gcgagggcgt tgacgccgtc gttggtgagc caggcgtggc
     1321 cggccacgag ccgggtgcgg gtgccgcagc ggtggacggc gcgctcggtg gtggcgtcgc
     1381 aggcgggaca atggaacctg ccctgcaccg tggcgcccgc caccgagtcg gcgagcatcc
     1441 cgatggcccc gatcgccacg gccgccacga gcaggcgggc gtcgtggacg acgagcgctg
     1501 ccgcggctgc gaccacgaat gcgccgagca gcgcgcccag cgtgccgcgg gtggtcacgc
     1561 cgccgctggt gccggggggg accgccgcac cggagaggat atcgtgcggg aggccgcggc
     1621 tggtggcgcc gacggccgtg gcccaggtgt cggcggcggc ggcggcgagg gaggaggtga
     1681 tggcccagag cgcgggggac gggcggccgg cggcgagcag cgcggcgatg gcggcggcgc
     1741 cgccgttggc gagcacctgg cccgcgtctc ggcggccgcc cttggcctca ccggccgccg
     1801 ctgcgggatc gggcagcaca cgggaaacga tcgagcttga gaggaagaag gcgaagagcg
     1861 cggccgcacc ggcccaaccc gccgtccaga gcaccgccgt cccgaccgcc gccgccgcca
     1921 cggcgccccg acccgacagg gcccgtgcca gggcggcgag ggtggccacc agcgcggcga
     1981 tggccgccgc gagcggcagg ctcacgtcag tcggcgcgcc ggaggctcgc gagcaggcgc
     2041 tcgcggacat cctgcgggat gcgctggccg gcgcagctcc gcagcagttc acggaagcgt
     2101 tcctcgaacg tgacgtaccg gaggcagggc gagcatacct ccagatgccg ctgcacctcg
     2161 gccgcggtct cgggcgtcgc ctcgccccgg agcacgtcct gcagctcatc aagcatccgc
     2221 gagcagtcaa tgagctcgtg gctcgtcatg ttgcggcccc tcccttcagg tagccctgtt
     2281 ccagcgcgtg gtcgcgcagc gaccgctgga gcaggcgtcg cgcccggaac aggcgcgact
     2341 tgaccgtgcc caccggcacg tcgagcacct gcgcgacctc ggcgtacgcc agcccctcga
     2401 ggtcgctcag caccagtgcc tcccggaact cctccggcag cgcctcgacc gccttgagca
     2461 ccgtctcgtc caccagcttg gtgaagaacg tcgcctcggg atcggcctcc gcgacgtcgc
     2521 ggatgacgga atgccggtcg gcggcgtcga tatccatcgt gaccggttcc cgcttccggc
     2581 ggcggtagcc gctgatgaag gcgttgcgga ggatggtgag gagccaggcg cgcgcgttgc
     2641 tgccgggccg gaagccctcc cagccccgca gcgccttgag cagcgtctcc tgcaccaggt
     2701 cttcggcgtc ggcggcgttg ccggtcatcc gccgcgcgac gcggaacagc acgtcgaggt
     2761 gcggcaggac ctcggtctcg taggcggcgg tggcttcggt cattggctga aaggtccccg
     2821 gggcgggcgt gggaggcaat ggggccgtgc gtgaagcgtg gcgggtggcg aggggccggt
     2881 cgacccatcg ccatccgcgg gcccccgggc gccactcgcc ccgtgccgcc cgccacctgt
     2941 cactcgtcac tggtcacctg tcgcgttaga atccgtcacc gtgcctgtgt tccctgccac
     3001 cgagatcgat gccatcgtgc cgctcagcct cctcgaggcg atgcggaacg tggatactcc
     3061 cgtcgaggac ggcctcgagg agcttgccgg cgagatcgtg gccaagcgcc tgggtctctc
     3121 gccgacggtg gcggcgcaga tcgcccgcta cgactgggcc gcggggcggg gggcgccggt
     3181 gtcgcaggag gaggcgctcg cggtgttccg cctggtgggc cggcggccgg acgccgacct
     3241 cgtctttgcc gatgcggggc gccgcgccgc ccgccgagcg gcgcgcgagg cgggggtggc
     3301 gccccgggcg ctcctcggga tcgtccccga ggccgccgcc cgtcccctcg gcctcgggtt
     3361 ggcgcgccgc ctggcgcgcc gggtctttgc cggcgaactc accctcaagg acgccgaggc
     3421 gaccgcccag gttcccgcgc cgctcgggat cgacgacgtg cccgaaggcg gggcgtgcgg
     3481 cttctacggc gccgcgttcg ccgagctgct gcgggcgctc gccggattcg agggagccat
     3541 gcgccacatc cgttgccgcg cccgcggcga cgatcaatgc caatggcgcg ccagcgccgt
     3601 gggaggctat cagtgagtgg gatctccgcg atcgaccgtg cgcagctggc cgagggactg
     3661 gccgagctcg gcgccgacgg ctggctcctc ttcgactttc atggactgaa tcccgtggcc
     3721 ggccgggtgc tggcgctggg cgggctcggg tcccgccggc tcttcgtgct catcccgcgc
     3781 acgggcgccc cgacggccgt ggcgcacaag atcgagctgc agccgatgga ggggttcccg
     3841 ggcacgatcg tgccgtacgc ccgctgggag gaactgcacg ctgcgctggg tccgctggtc
     3901 aaggggaaga ccctcgcgat ggaggtctcg ccgcgggacg ccgtgcccta tctcgaccgg
     3961 atcccccacg gcgtgatcca gctcctcgag gggctcgggg caaagatcgt gtcgtcggac
     4021 cagctggtga cccggttcgc cgcgcgctgg tcggcggcag agctcgcgga tcacctggcg
     4081 gcggcggagg tcctggcgga tgtggcgaag gagcagctgg cgtgggcggt gacgcagggc
     4141 ggaaaggggc tcaccgagag cgccctgcag cggcgcaccg tggcggcgat ggaggcccgg
     4201 gggctccggc tcacgaccca tcccatcgtc ggcttcgggc cgaacgccgc caatccgcac
     4261 tatgagccgc acgaagggca ggaccggacc ctggcgccgg atgaagtcgt gctgctcgac
     4321 ctctggggtg gccggggtcg ggtggacacc gtgttcgcgg accagacctg gatgggattc
     4381 tcggggcggc gggtgccgtc caaggtccag caggtgtggg aggtcgtccg cgatgcgcgg
     4441 gatgccgccg tggcgctggt gcaggagcgg gtcgcggccg ggaagccgct ggcgggatac
     4501 gaggtggacc gcgcggcgcg cggcgtgatc gagcgggcgg ggttcggcgc gtcgttcgtg
     4561 caccggaccg gacattccat cgatcgggac ttgcacgggt ccggcccgca catggacgat
     4621 tttgagacgc acgacgagcg gcggctcatc ccgggtgtgg ggttctcggt ggagccgggc
     4681 gtctatctgg tcggggagtt cggcattcgc agcgaggtca acatgttctg gggtgagtcc
     4741 ggggcccggg tgacgccgac ggcaccgcag cgtgacctga tcctccccgg cgcgtgacgg
     4801 gcccggccac cggcaaattg cggtatgcag cggtcggggg tggtggtcgc ggggccatca
     4861 tccggtgttc cacggagtta cgtgatcggg cccggattgc gggtcatgcg gggcttgctt
     4921 gacaggtgac aagggtcact tcatattggc tatcccgacc tgccgcggtc gggttccaga
     4981 tacagttcag tctcagtttc ctcgacaagt cggttgcgtt ccctcgcggg ccaaacgctg
     5041 gcgtcctgcc gcccctatcc ggccaataag ccgcggaggg atggtgctgc gccggtttac
     5101 gtgacagggc agttccgttg ttcacgcctt tggaggaagt gctcatgatt caacgctgtg
     5161 tcagggccgt tgcacgcgtc gcgcacctgg ccaccctggc ggcggtcgcc ctggtactcg
     5221 gggcgggcgc cctctcggcg caggccaccg ccaagatcga agggcgggtc cgagactctt
     5281 ccggacagcc gatcgcgaat gcacaggtgt tcatcgttgg gtcggccttc tcggccctca
     5341 ccaacgcgca gggttactac ttcatcaaca acgtgccggt cggcaccatc tcgatgaagg
     5401 ccgccttcgt cggctaccag ccggtgcagg tgaacagcgt gcaggtcctg ggcggtcaga
     5461 cgatgaccca ggacttctcg ctgaaggcct cggtggtcac cgtccaggcc ctcgagatca
     5521 cggccgagca tccgctggtg ccgcgagacg cggtcacctc gaagagccgc atcgacggcc
     5581 agatggccga agcgctcccg gtcgaccggg tgtcgtcgct gctggcgctg cagccgggcg
     5641 tggtggcgag tgcgagcggc gggacgctgt caatccgcgg cggccgtccg accgaggccg
     5701 tgacgtacgt cgacggcgtg ccggtgaccc cgggctaccg gggcggcggc ttcgtcggct
     5761 cgggcggttc atccatcacg gtgaacgcca acggcctgca ggaggccaac gtcacctcgg
     5821 gcgcgacgag cgccgagttc ggcaacgcgc agtcgggcat cttcgacttc aacacccgct
     5881 cgggcggcca gaagctctcg gccaacctgg gggtcagcac cgaccagccc tttgcccgca
     5941 cctcgagcct ggggtacacc cagatccggg gtggcgtggg cggcccgatc ccgggcgtcg
     6001 cgaacctgac ctactacgtc agcgcgctgc tcgagggcca gcagtcggcc gcgaccgggt
     6061 tcgacgccaa caaggtgccg gtcttcgtgc cggccggcat cgacaagacc gtcgccgtgc
     6121 cgagcgcgta cggcgatccg ttcgccgaca cgagctacgt cccggtggag aag
//
LOCUS       DASUAP010000087        12865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1037924, whole genome shotgun sequence.
ACCESSION   DASUAP010000087 DASUAP010000000
VERSION     DASUAP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037924"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..482)
                     /locus_tag="VFU45_02125"
     CDS             complement(96..482)
                     /locus_tag="VFU45_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5357889.1"
                     /translation="MTIDFIATPSAPKAIGPYSQATVAHGFIYTAGQIALDPATAEVV
                     AGGVAEQTERVMKNLAAVLAAAGSSLQRVVKTTVFLVDMGDFAAMNEVYARHFGDHRP
                     ARSTVAVAALPRGVRVEIEVVAVSAK"
     gene            complement(551..1321)
                     /locus_tag="VFU45_02130"
     CDS             complement(551..1321)
                     /locus_tag="VFU45_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU5357890.1"
                     /translation="MLVDTHCHLAAEAFAADRPDVLRRARAAGVERVVVIGESPAAAE
                     TAFRLAESEPMVSVATGTHPHDATEWDPERAAWLRAALERPGAVALGEIGLDYHYDHS
                     PRDVQRRAFESQLALAVELGLPAVIHAREADADVLAVLRNAPRAPVILHSFSSGPALL
                     EGALALGALASFSGMVTFKSWTGDDLIRLVPLDRLLVETDAPYLAPVPHRGKRNEPGF
                     VRQVADRVAQVRGMPVEELIAATGANAARVFGLSTTLA"
     gene            complement(1322..2281)
                     /gene="secF"
                     /locus_tag="VFU45_02135"
     CDS             complement(1322..2281)
                     /gene="secF"
                     /locus_tag="VFU45_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU5357891.1"
                     /translation="MRFFHHAKYNFISVERWAWSITGAIILPGIVLLAARGLNYSVEF
                     TGGTLIQVATEKPAQFGPVRSALEKAGMDAPELQAFGTTGTEFTIRARVGETGSTVDA
                     NNTQLTLQAAATGLDQSIGAGHYKIEHTYAIGPKVGAELRSQALWLIFLSFFVVLIYL
                     AIRFEWRFGVAAVLATAHDIVATIAFISILHMEVSLVVVAAVLSMVGYSLNDTIIIFD
                     RVRENMHKYKRSDLYQVLNLSINETLPRSVLTHGTVLATLLSLAIFGGEVIRPFSLVM
                     FFGVFTGTFSSIFIASPILMYIERRWPGEDVKGARSVAVPQKA"
     gene            complement(2285..3955)
                     /gene="secD"
                     /locus_tag="VFU45_02140"
     CDS             complement(2285..3955)
                     /gene="secD"
                     /locus_tag="VFU45_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU5357892.1"
                     /translation="MFSTIRSRLIVIAVLALCSIWAIWPVKQVEDVVQPDGSVHQDTV
                     KRWRPSLGLDLQGGIHLGLELDTSKVASTNPSRDIDLALTVLRKRIDEFGVAEPVIQK
                     VGDSRIVVELAGIKDPDRAKSIVERSAFLEFRLTDKTDALTKALPAMDRVLRGLGLTA
                     KSTAGAPTATAQQNAVQNLLGGDSARKKGADSTAADTGAVAGGILEGLIQPANTGGFS
                     VPGEYMVPEGAYPRVDSLLSIPAVNALLPRGLTWRWLSAPISVGVAPYRFLYILQDKA
                     IMTGSNLEDAQAGTDPLTQAAVVDFQLDRAGGRKFGQETGRHVGDYMAILLDNRVQGR
                     PPVINSRIDRRGQITLGNKTIQEARDLALTLKAGALPVPLRIVEQQQVGASLGKDSIH
                     DGILAGVIGTAFVVLIMIGYYRLSGLLAVGGLLLYILFAIGGLAVVGATLSLPGLAGL
                     VLSIGIAVDANVLIFERIREELDRGKTVRLSIDEGFKHAMAAIIDSNVTTVLTALFLF
                     QFGTGPVKGFAVTLTLGIAASMVTSIFVVRTFYMIWLDRRPNLSELSI"
     gene            complement(3970..4914)
                     /locus_tag="VFU45_02145"
     CDS             complement(3970..4914)
                     /locus_tag="VFU45_02145"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU5357893.1"
                     /translation="MRPLPIGPEGSVVTVGTFDGIHVGHRAVLDEISRRAVAAGRKSV
                     LVTFEPHPLAVVNPQLAPPLLTTGGERQEALALTALDYVLLLAFDRRMAAMAPEEFVA
                     RILVGMLDVRELVIGHDHGFGRGRSGDVETLRRLGGKYGFAVDVVGAVEVAGHPVSST
                     LVRRAVGGGDLDGAARLLGRRYQVVGKVEHGAGRGRTIGIPTANLGGVPPGKLLPPDG
                     VYAAWVETRRGRFGAMLNQGPRPTFQDGRRNLEVHLFGFAGDLYGEWIKVEWVRRLRD
                     VERFSSIDALKAQLSQDRTRAEAALADVGRGEQRVPHP"
     gene            complement(4911..5777)
                     /gene="truB"
                     /locus_tag="VFU45_02150"
     CDS             complement(4911..5777)
                     /gene="truB"
                     /locus_tag="VFU45_02150"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU5357894.1"
                     /translation="MTGALLIDKPAGPTSHDVIARVRQALGVRAVGHTGTLDPFATGL
                     LVVLVGRATRLARYFDDLPKVYRATVRLGQGSDTDDATGSLGSRVAGREFSTDEVAGA
                     LRSFVGTYAQRPPAYSAKKIEGVRAYALARKGAEVSLPLVEVTIHSAELLGYAAPDVE
                     IRVSVSGGTYVRALARDLGERLGVPAHCAALRREAIGPLRVDRAVAPEAVTRATLLLT
                     PAEVLGHLPSFEVGAEAAEALRHGRQVLMQGSPAPSASPALSALSAGALVAVGHRTPE
                     GLFQPDVVMEGA"
     gene            complement(5774..6148)
                     /gene="rbfA"
                     /locus_tag="VFU45_02155"
     CDS             complement(5774..6148)
                     /gene="rbfA"
                     /locus_tag="VFU45_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU5357895.1"
                     /translation="MPKSRATTRRPEQVAETVRQVLAEALTRGEIRDPRVGFVTVTGV
                     RVTNDLSHARVLVSCLGEPADRERSIEGLKSAAKYLRARLGEVLATRVVPELHFELDD
                     GLERAARIDHLIAQLHREPEEP"
     gene            complement(6216..8996)
                     /gene="infB"
                     /locus_tag="VFU45_02160"
     CDS             complement(6216..8996)
                     /gene="infB"
                     /locus_tag="VFU45_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5357896.1"
                     /translation="MGKTRVHELAAEFGVSAEQLMALLKEMNIFVRSHMSALEPDQVA
                     AVRVRWEREKRKAAEPEKPKKGRRKAAAKAPEPAPVAEVKPVKRRRTAAEVAQQEAVL
                     EAERQREAELAAKQALFEMELPRAEDEKPALPASLEERAKLLFKDLPLAPPEAETDEA
                     APAAASEPAAPPAPPRIPRIPPRPAETSAPTPTVNRAPFIPPRIARPAAGGAPARGPG
                     GPPSRGPAGAGGAGRGGPRPVFSSAAPGAPGSAERKPGPGGRDGKKSDPYADERGRRK
                     KGKRGAVDQDAVQANILKTLQGMKGPVRKGGRRADGPSYQELQAGLAAEEAAREKTRI
                     RVNEFVSVSELAGTLKVPATQIVQFAFKELGLMVTVNQRLDFDQIELIASAFGFEAVK
                     ESEYEAEQDVVQEADAEGSLKPRPPVVTIMGHVDHGKTSLLDYIRKANVVAGEAGGIT
                     QHIGAYHVELPGGKAITFLDTPGHQAFTAMRARGAQVTDIVVLVIAADDQVMPQTIEA
                     ISHAKNAGVPMIVAINKIDLPGANVQKVKQDLLQHQVVLEEFGGNVLSAPISAKKGTG
                     IPELLEQILLQAEILDLKANPDAKMQGAVIEATLDPGKGPLATILVQKGTLKAGADFI
                     CGRFSGRVRAMYDERGKAVKSAGPSIPVQILGFEGVPDAGDSFQVVDDAVQAREIAQK
                     RQRLEREAHSRRTARSSSLEDFSRQLAEGQVSALNIIIKADQGGPAEALADALAQLST
                     PEVRVDVVHRGVGAITESDVLLAKTSGAIILGFHVRPDSNARAAAERESVDVRTYRII
                     YEAVDDVRNALEGLLKPEEKEVVLGEAEVLQLFKVSKVGTIAGCVVRNGVIQRTAKAR
                     VVRDGTTVYTGTLSSLKRFKDDAKEVKEGLECGIGIENFNDLKAGDRIESFRVEEVKR
                     TLAASSAGGEKA"
     gene            complement(9007..9318)
                     /locus_tag="VFU45_02165"
     CDS             complement(9007..9318)
                     /locus_tag="VFU45_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HEU5357897.1"
                     /translation="MTGGGLLGLLALGRRAGQLDLGVDAVRAGLQAGRVRCVVLATDA
                     SPRALEKVMRLANGIRVPLVAGPDAESLGARLGRPPLMAVGVKDRALADGIIRQAAPW
                     A"
     gene            complement(9320..10639)
                     /gene="nusA"
                     /locus_tag="VFU45_02170"
     CDS             complement(9320..10639)
                     /gene="nusA"
                     /locus_tag="VFU45_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU5357898.1"
                     /translation="MAGSAEMLTALRELTNAKQLERAELIDLIRDGIHAALMKRYGPN
                     VKFELSLDELQGTIRVIRLRTVADPVEDESFQVSLEEAQFEDPEFAVGDVLEEEVPFS
                     EFGRGAVQAAKQRIIQRVREGERNRIREEFTDKVGELLSGEIQQIERGKLVLMLNKFR
                     EAEAIIPYREQNHREHFHQGDTIRAVLKKIEETPKGPRLILSRADPLFVKALFKLEVP
                     EIQQQIVEIRAWAREVGSRTKVAVISRDDAIDPVGACVGLKGSRVQAVVNELGGERID
                     IVPWSPDPERFARLALAPARVAKVFSEPETKTIQAVVDEDQLSLAIGRNGQNVRLASE
                     LTGWKIDLYSSREWLERGGEAPLFAPLAPEDEAATKIPLAELTGLSPEVTAALAGAGV
                     TMLNQLLDLDREDIAKLAGMTPESTEQLLAYLQELTTEDTGEEPAAS"
     gene            complement(11181..11540)
                     /locus_tag="VFU45_02175"
     CDS             complement(11181..11540)
                     /locus_tag="VFU45_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU5357899.1"
                     /translation="MERYERFAPWQAAHRLILTVYRHTRRWPPDERFGLVGQARRAAV
                     SVASNIVEGSCRRGTKEFRRYLDISFASLAEVGYQLRLARDLGYEASEAWTAVEAERI
                     ATVKALWNLMRGLDAHR"
     gene            complement(11700..12470)
                     /locus_tag="VFU45_02180"
     CDS             complement(11700..12470)
                     /locus_tag="VFU45_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEU5357900.1"
                     /translation="MIRETPIPGDIPRYELTDWRERYGVVAGITGRPFDLGLWSDQPV
                     GQVMTRWRQFRDGLGFSRYVLGHQCHGVDVVWTGSGQEAGGERADSGRTNSLTIVDRI
                     DGHATASAGTLLLVTVADCIPIYLLEPRKKVLALLHSGWRGTAGGILGKGLDLLRQRA
                     GVEPRDLVMHLGVGICGKCYEVGAEVIEGVGLRADGDGPWHVDLRERLAHQARAFGVR
                     EISISGWCSAHDRDRFFSHRASGGRDGRMVGYLGRAGE"
     gene            complement(12650..>12865)
                     /locus_tag="VFU45_02185"
     CDS             complement(12650..>12865)
                     /locus_tag="VFU45_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5357901.1"
                     /translation="CDPHRAIVSGPSRLHGATVESPDIRAGMAILLAALCAEGKSVIN
                     NIGQIERGYERIGERLKALGADIERVE"
BASE COUNT         1873 a         4606 c         4468 g         1918 t
ORIGIN      
        1 gagtgggtga cgagtggtga gtgcgcggga agcacgcacc ggccgcacct gactcgtgac
       61 ccatcacccg cgccccaccc gccagtcgct acccgctact tggcgctcac cgcgaccact
      121 tcgatctcga cccgcacgcc cctcggcagc gccgccacgg ccactgtcga gcgggccggc
      181 cggtggtcgc cgaagtgccg ggcatagacc tcgttcattg cggcgaagtc gcccatgtcg
      241 accaggaaga cggtcgtctt gacgacccgc tggaggctcg agccggcggc cgcaagcacc
      301 gccgcaaggt tcttcatgac gcgctcggtc tgttccgcga ccccgccggc cacgacctcg
      361 gcggtggcgg ggtcaagcgc gatctggccg gccgtgtaga tgaatccgtg cgccacggtg
      421 gcctggctgt aggggccgat ggccttgggc gcggagggtg tggcgatgaa gtcgatggtc
      481 atggaagtaa gaagtgagaa gtaagaagtg agaagtgcga cccgcgacct aagatgccgc
      541 aatgccggcg ttacgcaagc gtcgtggaga gcccgaagac ccgggcggcg ttggcgccgg
      601 tggcggcgat cagctcctcg accggcatcc cccgcacctg ggcgacccga tcggccacct
      661 gacggacgaa gcccggctcg ttccgcttgc cacggtgcgg caccggggcc aggtaggggg
      721 cgtcggtctc caccaggagc cggtcgagcg gcaccaggcg gatgaggtcg tcccccgtcc
      781 agctcttgaa cgtcaccatc ccgctgaagg aggccagcgc acccagcgcc agcgcgcctt
      841 cgaggagcgc cgggccgctg gagaacgagt gcaggatgac cggcgcccgc ggcgcgttcc
      901 gcagcacggc aaggacgtcg gcgtcggcct cgcgggcgtg gatcacggcc gggaggccga
      961 gttcgacggc cagcgcgagc tgggactcga aggcgcgacg ctggacgtcc cggggcgagt
     1021 ggtcgtagtg ataatcgaga ccgatctcgc ccagcgccac cgcgcccgga cgttcaagcg
     1081 cggcgcggag ccaggcggca cgctccgggt cccactcggt ggcgtcgtgc gggtgggtgc
     1141 ccgtcgccac cgagaccatc ggctccgact ccgccaggcg gaaggccgtc tccgccgccg
     1201 cgggggattc gccgatcacg accacccgct ccactcccgc cgcacgagcg cggcggagga
     1261 cgtcgggccg gtccgccgcg aacgcctccg ccgccaggtg acagtgggtg tcgaccagca
     1321 ctcaggcctt ctgcggcacc gccaccgacc gggccccctt cacgtcctcg ccgggccagc
     1381 gccgctcgat gtacatcagg atcggcgacg cgatgaagat cgacgagaag gtgccggtga
     1441 agacgccgaa gaacatcacc agcgagaagg gccggatcac ctcgccgccg aagatggcga
     1501 gcgagaggag cgtcgcgagc acggtgccgt gggtgagcac gctccgcggc agcgtctcgt
     1561 tgatcgacag gttcagcacc tggtagaggt cgctccgctt gtacttgtgc atgttctcgc
     1621 ggacgcggtc gaagatgatg atggtgtcgt tgagcgaata cccgaccatc gagagcaccg
     1681 cggccaccac caccaggctc acctccatgt gcaggatgga gatgaacgcg atcgtggcca
     1741 cgatgtcgtg ggcggtggcg agcaccgcgg ccacgccgaa gcgccattcg aaccggatgg
     1801 cgaggtagat cagcaccacg aagaacgaca ggaagatgag ccagagcgcc tggctgcgga
     1861 gctcggcccc gaccttcggc ccgatggcgt aggtgtgctc gatcttgtag tgccccgcgc
     1921 cgatggactg gtcgagcccg gtggcggcgg cctgcagggt gagctgcgtg ttgttggcgt
     1981 cgacggtgct gccggtctcg cccacgcggg cgcggatggt gaactccgtc ccggtggtgc
     2041 cgaaggcctg gagctccggc gcgtccatcc ccgccttctc gagcgcggag cggaccggcc
     2101 cgaactgggc cggcttctcg gtggcgacct ggatcagcgt gccgccggtg aactcgacgc
     2161 tgtaattgag cccccgcgcc gcaagcagga cgatgcccgg caggatgatg gcgcccgtga
     2221 tgctccaggc ccaccgctcg accgagatga agttgtactt ggcgtgatgg aagaatcgca
     2281 tgggtcagat gctcaactcg gagaggttcg gccggcggtc cagccagatc atgtagaacg
     2341 tacggaccac gaagatcgac gtgaccatcg acgccgcgat gccgagcgtc agggtcaccg
     2401 caaagccctt gaccgggccg gtgccgaact ggaagaggaa cagcgcggtg agcaccgtgg
     2461 tcacgttgga gtcgatgatc gcggccatcg cgtgcttgaa gccctcgtcg atcgagagcc
     2521 gcacggtctt gccgcggtcg agctcctcac ggatgcgctc gaagatcagc acgttggcgt
     2581 cgaccgcgat gccgatcgag agcaccaggc ccgcgaggcc cggcaggctc agcgtggcgc
     2641 cgacgacggc caggccgccg atggcaaaga ggatgtagag caggagcccg cccaccgcca
     2701 ggagcccgct cagccggtag tagccgatca tgatgagcac cacgaacgcc gtgccgatga
     2761 cgccggccag gatgccgtcg tggatcgagt ccttgccgag gctggcgccc acctgctgct
     2821 gctccacgat ccgcagcggc accggcagcg cgccggcctt gagcgtgagc gccaggtcgc
     2881 gcgcttcctg gatggtcttg ttgccgaggg tgatctggcc ccggcggtcg atgcggctgt
     2941 tgatgaccgg cggccgcccc tgcacccggt tgtcgaggag gatggccatg tagtcgccca
     3001 cgtggcgccc ggtctcctgg ccgaacttcc ggcccccggc gcggtcgagc tggaagtcga
     3061 ccacggcggc ctgggtgagc ggatcggtcc ccgcctgcgc atcctcgagg ttggacccgg
     3121 tcatgatggc cttgtcctgg aggatgtaga ggaaccggta cggcgccacg cccacgctga
     3181 tcggcgcgga gagccagcgc caagtgaggc cgcgggggag gagcgcgttg accgccggga
     3241 tcgacagcag cgagtcgacc cggggatagg cgccctcggg caccatgtac tcgcccggca
     3301 ccgagaagcc gccggtgttg gcgggctgga tcagcccctc gaggatcccg ccggccacgg
     3361 ccccggtgtc ggcagcggtg gaatcggcgc ctttcttcct ggcgctgtcg ccgccgagga
     3421 ggttctgcac cgcgttctgc tgcgcggtcg cggtcggcgc cccggcggtc gacttggcgg
     3481 tgaggccgag cccgcggagc acccggtcca tggccggcag cgccttggtg agcgcgtcgg
     3541 tcttgtcggt gaggcggaac tcgaggaacg cgctccgctc cacgatggac ttggcgcggt
     3601 cggggtcctt gatgcccgcc agctcgacga cgatgcggct gtcgcccacc ttctggatga
     3661 cgggctcggc cacgccgaac tcgtcgatcc gcttgcggag cacggtgagc gcgaggtcga
     3721 tgtcgcgcga gggattggtc gaggccacct tcgaggtgtc gagctcgagc ccgaggtgga
     3781 tgccgccctg gaggtcgagg ccgaggctcg gccgccagcg cttgacggtg tcctggtgga
     3841 cactgccgtc gggctgcacc acgtcctcga cctgcttgac gggccagatg gcccagatcg
     3901 aacagagcgc aaggacggcg atgacgatca gtcggctgcg gatggttgag aacatcgctc
     3961 cgagacgcgt taggggtgag ggaccctctg ctccccgcgc ccgacgtcgg ccaacgcggc
     4021 ctcggcccgg gtgcggtcct gggacaactg cgccttcagc gcgtcgatcg agctgaaccg
     4081 ctcgacgtca cgcaggcggc ggacccattc caccttgatc cactcgccgt agagatcgcc
     4141 cgcgaagccg aagaggtgga cctcgaggtt ccgccgcccg tcctggaagg tgggccgcgg
     4201 gccctggttg agcatcgccc cgaagcggcc ccggcgggtc tcgacccagg cggcgtagac
     4261 cccgtcgggc gggaggagct tgcccggcgg caccccgccc aggttggcgg tggggatccc
     4321 gatggtgcgc ccccgccccg ccccgtgctc caccttcccc accacctggt agcgccggcc
     4381 gaggagccgc gcggcgccgt cgaggtcgcc gccgcccacc gcgcgccgca ccagcgtgct
     4441 cgacaccggg tgccccgcca cctccaccgc ccccaccacg tccaccgcga agccgtactt
     4501 cccgcccagc cgccgcaggg tctcgacgtc gccgctgcgc ccccggccga agccgtgatc
     4561 gtggccgatg acgagctcgc gcacgtccag catcccgacc aggatcctcg ccacgaactc
     4621 ctcgggggcc atggccgcca tccggcggtc gaaggcgagc aggagcacgt agtcgagcgc
     4681 cgtcagcgcc agcgcctcct gccgttcgcc cccggtggtg aggagcggcg gcgcgagctg
     4741 cgggttcacc accgcgagcg gatgcggctc gaaggtgacc agcacgctct tccgccccgc
     4801 cgccacggcc cgccggctga tctcatccag caccgcgcgg tgcccgacgt ggatcccgtc
     4861 gaacgtcccg acggtgacca ccgacccttc cggcccgatc ggcagcggcc tcacgccccc
     4921 tccatcacga cgtccggctg gaagagcccc tccggcgtcc ggtgccccac cgcgaccagc
     4981 gcaccggcgg acagggcgga cagggcgggc gaggcggacg gggccggcga tccctgcatc
     5041 aagacctggc gcccgtggcg cagcgcttcc gccgcctccg cgcccacctc gaagctcggc
     5101 aggtgcccca gcacctcggc cggcgtgagg agcagtgtgg cccgggtgac cgcctccggc
     5161 gccaccgccc ggtccacccg gagcggaccg atcgcctcgc ggcggagcgc ggcgcagtgc
     5221 gccggcactc ccagccgctc gcccaagtcg cgggccagcg cccgcacata cgtcccgccg
     5281 ctcaccgaca cccggatctc gacatcgggc gccgcgtacc cgagcagctc cgccgagtgg
     5341 atggtcacct cgaccagcgg gagcgatacc tccgcgccct tccgcgccag cgcgtaggcg
     5401 cgcactcctt cgatcttctt ggccgaatac gccggcggcc gctgcgcgta ggtcccgacg
     5461 aagctccgga gcgcgcctgc cacctcgtcc gtgctgaact cacgacccgc gacccgcgac
     5521 ccgagacttc ccgtcgcgtc atcggtgtcc gatccctgcc ccagccgcac cgtcgcccgg
     5581 tagaccttgg gcaggtcgtc gaagtagcgc gccagccggg tggcccggcc caccagcacc
     5641 accaggagcc ccgtcgcgaa gggatcgagg gttcccgtgt gccccaccgc acgcaccccc
     5701 agcgcctgcc gtacccgggc gatgacgtcg tgcgacgtcg gccccgccgg cttgtcgatg
     5761 aggagcgcgc cggtcagggc tcctccggct cccgatggag ctgggcgatc aggtggtcga
     5821 tccgggccgc acgctcgagc ccgtcatcga gctcgaagtg gagctccggc accacgcgcg
     5881 tcgccagcac ctcgccgagc cgggcacgaa gatacttggc cgcgctcttg agcccttcga
     5941 tggagcgctc ccggtccgcc ggttcgccaa ggcagctcac caggacccgg gcgtggctca
     6001 ggtcattggt gacccgcacg ccggtgacgg tgacgaaccc gacccgcggg tcgcggatct
     6061 cgccgcgcgt gagcgcctcg gccaggacct gacggacggt ctcggccacc tgctcggggc
     6121 ggcgggtagt ggcgcggctc ttgggcatag gggtcgtggg tcgtgggtcg tgagtcaagg
     6181 gatcatgatg acccgtgacc cgcgacccgt gacctctacg ccttctcccc gcccgccgac
     6241 gacgcggcga gggtccgctt cacttcctcg acccggaacg actcgatccg gtcgccagcc
     6301 ttgaggtcgt tgaagttctc gatgccgatg ccgcactcga gtccctcctt gacctccttc
     6361 gcgtcgtcct tgaagcgctt gagcgagctc agcgtgccgg tgtagacggt ggtgccgtcg
     6421 cggaccacgc gcgccttggc ggtgcgctgg atgaccccgt tacggaccac gcagccggcg
     6481 atggtgccga ccttgctcac cttgaagagc tgcagcacct cggcctcgcc caggaccact
     6541 tccttctctt cgggcttgag cagcccctcg agcgcgttgc ggacgtcgtc gaccgcctcg
     6601 tagatgatgc ggtaggtgcg cacatccacc gactcgcgct cggcggcggc gcgggcgttg
     6661 gaatcgggcc gcacgtggaa gccgaggatg atggcccccg acgtcttggc gaggaggacg
     6721 tcgctctcgg tgatggcgcc gacgccgcgg tgcaccacgt ccacccgcac ctcgggggtc
     6781 gagagctggg ccagcgcgtc ggccagcgcc tcggccgggc cgccctggtc ggccttgatg
     6841 atgatgttga gcgcgctcac ctggccttcg gccagctggc ggctgaagtc ctcgagcgag
     6901 ctcgagcgcg ccgtgcggcg gctgtgggcc tcgcgctcca ggcgctggcg cttctgcgcg
     6961 atctcgcgcg cctgcaccgc gtcgtccacc acctggaagg agtcgccggc gtcgggcacg
     7021 ccctcgaagc ccaggatctg caccgggatc gacgggcccg ccgacttgac cgccttgccg
     7081 cgctcgtcgt acatcgcgcg gacgcggccg gagaagcggc cgcagatgaa gtcggcaccg
     7141 gccttgagcg tgcccttctg caccaggatg gtggccagcg ggcccttgcc ggggtcgagg
     7201 gtggcctcga tgaccgcgcc ctgcatcttg gcgtcggggt tggccttgag gtcgaggatc
     7261 tcggcctgca ggaggatctg ctccaggagc tcggggatcc cggtgccctt cttggcgctg
     7321 atcggcgcgg agaggacgtt gccgccgaac tcctcgagca ccacctggtg ctgcaggagg
     7381 tcctgcttga ccttctgcac gttggcgccg gggaggtcga tcttgttgat agccacgatc
     7441 atcggcacgc cggcgttctt ggcgtggctg atggcctcga tggtctgcgg catcacctgg
     7501 tcgtcggcgg cgatgacgag caccacgatg tcggtgacct gcgcgccgcg ggcccgcatg
     7561 gcggtgaagg cctggtggcc cggggtgtcg aggaaggtga tggccttccc gcccggcagc
     7621 tcgacgtggt aggcgccgat gtgctgggtg atcccgcccg cctcgccggc gacgacgttg
     7681 gccttccgga tgtagtcgag gaggctcgtc ttgccgtggt cgacgtgacc catgatggtg
     7741 acgaccggcg gccgcggctt gagcgacccc tcggcgtcgg cctcctgcac cacgtcctgc
     7801 tcggcctcgt actccgactc cttgaccgcc tcgaagccga acgccgaggc gatgagctcg
     7861 atctggtcga agtcgagccg ctggttgacc gtcaccatca ggcccagctc cttgaacgcg
     7921 aactggacga tctgggtggc gggcaccttg agcgtgccgg cgagctcgct caccgacacg
     7981 aactcgttga cccggatccg ggtcttctcg cgcgcggcct cttcggccgc cagcccggcc
     8041 tgcagctcct ggtaactcgg cccgtcggcg cggcggccgc ccttccggac ggggcccttc
     8101 atcccctgca gcgtcttcag gatgttggcc tgcaccgcgt cctggtccac ggcgccgcgc
     8161 ttccccttct tgcggcggcc gcgctcatcg gcgtacggat cgctcttctt gccgtcccgg
     8221 ccgccggggc cgggcttgcg ctcggcgctc ccgggggcgc cgggggccgc gctcgagaag
     8281 accggccgcg gaccgccgcg cccagcccca ccggcgccag cgggcccgcg cgaaggggga
     8341 ccaccgggac cgcgggcggg tgcgccaccg gcggcaggac gcgcgatgcg cggcggaatg
     8401 aacggcgcgc ggttgacggt cggcgtcggg gccgaggtct ccgcggggcg cggcgggatg
     8461 cgtgggatac ggggcggcgc cgggggtgct gcgggctccg acgcggcggc cggggccgcc
     8521 tcgtccgtct ccgcttccgg cggcgccagc gggaggtcct tgaagaggag cttggcgcgc
     8581 tcctcgagcg aggcggggag ggcgggcttt tcgtcctccg cgcgcggcag ctccatctcg
     8641 aagagcgcct gcttggcggc gagctccgcc tcgcgctggc gctccgcctc gaggacggcc
     8701 tcctgctggg ccacctcggc ggcggtgcgc cggcgcttga ccggcttcac ttccgcgacc
     8761 ggcgccggct cgggcgcctt ggcggcagcc ttgcggcgcc ccttcttggg cttctccggc
     8821 tcggccgcct tccgcttctc gcgctcccag cggacacgca ccgccgccac ctggtcgggc
     8881 tcgagcgccg acatgtggct gcgcacgaag atgttcatct ccttgagcag cgccatcagc
     8941 tgctcggcgg agacgccgaa ttctgccgca agttcgtgga ctctcgtctt ccccacgctg
     9001 cctccgttag gcccagggcg cggcctggcg gatgatcccg tcggccaggg cccggtcctt
     9061 caccccgacc gccatcagcg gcggccggcc caagcgggcg ccgagggact cggcgtccgg
     9121 tccagcgacg agcggcaccc ggatgccgtt cgccagtcgc atcacctttt ccaatgcgcg
     9181 ggggctcgcg tcggtcgcca gcaccacgca gcgcacccgt cccgcctgca gtcccgcccg
     9241 caccgcgtcc accccgaggt cgagctgccc cgcccgccgg ccgagggcca gcaacccgag
     9301 gagcccgccg cccgtcacct caggaggccg ccggctcctc gccggtgtcc tcggtggtca
     9361 gctcctgcag gtacgccagg agctgttcgg tcgattccgg cgtcatcccc gccagcttgg
     9421 cgatgtcctc gcggtcgagg tcgaggagct ggttgagcat cgtgaccccg gcgccggcga
     9481 gcgccgcggt gacctcgggc gagagcccgg tgagctcggc cagcgggatc ttggtggccg
     9541 cttcgtcctc gggggccagg ggcgcgaaga gcggggcctc gccgccgcgc tcaagccact
     9601 cgcggctcga gtagaggtcg atcttccagc cggtgagctc ggaggcgagc cggacgttct
     9661 gcccgttccg cccgatggcg agcgagagct ggtcttcgtc gaccacggcc tggatggtct
     9721 tggtctccgg ctcggagaac accttggcga cgcgcgccgg ggcgagcgcc agccgggcga
     9781 agcgctccgg gtcgggactc cacggcacga tgtcgatccg ctccccgccc agctcgttga
     9841 ccaccgcctg gacgcgcgaa cccttgagcc ccacacaggc gcccaccggg tcgatggcgt
     9901 cgtcgcggga gatgacggcg accttggtgc ggctccccac ctcgcgggcc caggcgcgga
     9961 tctcgacgat ctgctgctgg atctcgggga cctcgagctt gaagagcgcc ttgacgaaga
    10021 gcgggtcggc gcgcgacagg atgaggcgcg ggcccttcgg ggtctcctcg atcttcttca
    10081 gcaccgcgcg gatcgtgtcg ccctggtgga agtgctcccg gtggttctgc tcccggtagg
    10141 ggatgatcgc ctcggcctcg cggaacttgt tgagcatcag caccagcttg ccacgctcga
    10201 tctgctggat ctcgccggaa aggagctcgc ccaccttgtc ggtgaactcc tcgcggatcc
    10261 ggttgcgctc cccttcacgg acgcgctgga tgatccgctg cttggccgcc tgcaccgcgc
    10321 cgcggccgaa ctcggagaac gggacttcct cctccagcac gtcgccgacg gcgaactcgg
    10381 ggtcctcgaa ctgggcctcc tcgagggaca cctggaagct ctcgtcctcg accggatcgg
    10441 ccacggtgcg gagccggatc acccggatgg tgccctggag ctcgtcgagg gagagctcga
    10501 acttcacgtt gggcccgtag cgcttcatca gcgccgcgtg gatcccgtcg cggatcaggt
    10561 cgatcagttc cgcgcgctcg agctgcttcg cattggtgag ctcgcgcagc gccgtcaaca
    10621 tctcagccga accggccata cactctcctg agttccagca cgctcgcgac cgggcaggtg
    10681 tcccgaccgc cgccgccacc tcgtaagggc gcagcacgct gcgcccgcaa atacggatgc
    10741 tccgcggtca ccccgcggcc cggccggagc cggacgcgtg tcgcaccttg tgggaaggag
    10801 cgctcggaag ggccgtccag ccgaccacac ccacgggccg gacgtcgcga ggtgccgaaa
    10861 acaaaaaatg tggggagacc gtcccccaca tccgcctgca gtccaccccc cggtcccttc
    10921 aggggcaggg cgtaagtata ggcaccgggg tgggataggt caattggcgg gcgaggggcg
    10981 ggcgaggggc gggcgaaaag cgggcaaaga gcgggctaga agcgggctag aagcgggcga
    11041 aaagcgggcg aaaagcgggc aaagagcggg caacgagcgg gctaagagcg ggcttgaagc
    11101 gggctaagag cgggctaaga gcgggcgagg ggcgggcgaa aagcgggctg gaagcaggcg
    11161 aagagcgggt gagagaagcg ttaacggtgt gcatccaacc cgcgcatcaa gttccagagc
    11221 gccttgaccg tggcgatgcg ctcggcctca accgcagtcc acgcttcgga tgcctcgtag
    11281 ccgaggtcac gggcaaggcg caactgatat ccgacctcgg ccagcgaagc gaacgagata
    11341 tcgaggtacc tgcggaactc cttcgttccg cgacggcacg agccttccac gatgttggag
    11401 gccacgctga ccgccgcgcg ccttgcctga ccgaccaacc cgaagcgttc atcaggaggc
    11461 cacctccgcg tgtgccggta aaccgtcagg atcaggcggt gcgcagcttg ccagggcgca
    11521 aatcgttcgt aacgctccat ccggccaagg tacgtggaga acttgaacca agcggcatca
    11581 caaacgctcg cggtggatca gaagatcgcg aaccctgccg cgctctccgc ccgctcttcg
    11641 cccgcttccg gcccgctgtt cgcccgctct tcgcccgctt ccagcccgct cttcgcccgc
    11701 tattcccccg cccgccccaa gtaccccacc atcctcccat ccctgccccc cgacgcccgg
    11761 tgactgaaga accggtcccg atcgtgggca ctgcaccacc cggagattga aatttcccgg
    11821 acgccgaacg cccgggcttg gtgcgccagg cgttcccgca ggtccacgtg ccagggcccg
    11881 tccccgtccg cccgcaaccc caccccctcg atgacctcgg caccgacctc gtaacacttc
    11941 ccgcagatcc cgacgcccag gtgcatcacc aggtccctgg gctccacccc ggcgcgctgg
    12001 cgcagaagat cgagcccctt ccccaggatc ccgccggcgg tgccccgcca cccggagtgc
    12061 aggagcgcca ggaccttctt ccgcggctcg aggagataga tcgggatgca atcggccacg
    12121 gtcacgagga ggagcgtccc cgcgctcgcc gtggcgtggc cgtcgatgcg gtcgacgatc
    12181 gtcagtgagt tggtccgccc gctgtcagcc cgttcgccgc ccgcttcctg cccgcttccc
    12241 gtccacacga catccacccc atgacactga tgcccaagca cgtatcgcga gaacccgagc
    12301 ccatcccgga actgccgcca gcgggtcatc acctgcccca cgggctggtc ggaccagagg
    12361 ccgaggtcga aggggcgccc cgtgatcccg gcgacgacgc cgtagcgctc gcgccagtcg
    12421 gtcagctcgt agcgggggat gtcaccgggt atcggcgttt cgcggatcat tgcgcacctt
    12481 ggaatactgt gggaccacgg agggcacgga ggagcacgga gacacacgga gaactgccgt
    12541 tgttgtggtc atgcgcgcgg agctccaccc gccaggccac atcgcaaagc tgttctccgt
    12601 gcctcttcgt ggactccgtg ccctccgtgg tcccacttca gttcccgatt tactcgaccc
    12661 gctcgatgtc cgcgccgagc gccttgagcc gctcgccgat ccgctcatac ccccgctcga
    12721 tctgcccgat gttgttgatg acgctcttcc cctcggcgca gagggcggcg aggaggatgg
    12781 ccatccccgc gcggatgtcg ggcgattcga ccgtcgcgcc gtgcagtcgc gatgggcccg
    12841 agacgatggc gcggtgcggg tcgca
//
LOCUS       DASUAP010000088         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_847136, whole genome shotgun sequence.
ACCESSION   DASUAP010000088 DASUAP010000000
VERSION     DASUAP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847136"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFU45_02190"
     CDS             complement(<1..651)
                     /locus_tag="VFU45_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5357902.1"
                     /translation="MPILVHLALIAVQVLFSSLAIAGKYVLGEVPPVVLVLARVTGAA
                     CVLLVVHRTSGAARVTDRMDLLRLCGLGILGIVANQTFFLLGLARTTAVNASVLVATV
                     PVFTVLNAMLIREEAPNARKILGILAAAAGSVWLIGPGRLTLRSETAVGDLLILAGMY
                     AYAQYLVLGKRMLRRYDPLTVTTYVMTFAAIGVLPLGLPALAHLDIGAVTPITWMWV"
     gene            complement(718..>2546)
                     /locus_tag="VFU45_02195"
     CDS             complement(718..>2546)
                     /locus_tag="VFU45_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5357903.1"
                     /translation="PKLSAAFYHGGEPIRVIRPFLDGEVERPFLLAMTAAGQSALNIR
                     GLDTVIIYDARYGNVVDRGRNVLHRMYLGANEILQMAGRVHGRVPGGEVVILSDRDLV
                     FEELRPTPPEFQLAGDAERVAVTCAALGVDARDLDLPVPLDRAAYVRAVRTLTSRGLM
                     AEGKLTPYGRQVEAMPVDRAWGELLVHADPEVLPIVAVCANIDSLHRITREERDLHGV
                     VVRGSDHLTAYNLFAEAVNQHGYVGQVYGLPRHLFEEGLEAWAERRGVLVKAIEDIAL
                     GAASIYRAMEQELPPRLPYAGHDLQRRWNELVARVMPFDLVIDERTADGQEARVSKTS
                     VAGNWGAVAGNLRYFGDRFGTPRASIEGTTLSYDEVRAHAQWERARVVVTGPRKHQHL
                     ALTHKLTYFGFDLEDSLEPIRGAVPAEHRAAAGEALVEALVAGETVHPRQGGIRRALL
                     ELDELWRRSGGTLAAAGPDAVRSALREQVVEVASWEEFLARRISLDPAERVDPEVRER
                     LLALPSSARVLGDAVPIEYEVEPQVGGVARLRLREGQARRLQDREVPVLDRPVRFAVA
                     RGNHPLLRADTLGALRELLREGGARSEGRRQRGRPHPRRRHR"
BASE COUNT          374 a          931 c          904 g          337 t
ORIGIN      
        1 gacccacatc caggtgatcg gcgtgacggc gccgatgtcg aggtgtgcga gcgcgggcaa
       61 tccgagcggg agcacgccga tggcggcgaa cgtcatcacg tatgtcgtga cggtgagcgg
      121 atcgtagcgg cgcagcatcc gcttcccgag caccaggtac tgcgcgtagg cgtacatccc
      181 ggccaggatc aggagatccc ccaccgcggt ctccgagcgg agcgtgagcc ggccggggcc
      241 gatcagccac accgagcccg ccgccgcggc caggatgccc aggatcttgc gggcgttcgg
      301 cgcctcttcc cggatgagca tcgcgttgag caccgtgaac accggcacgg tggccaccag
      361 cacgctcgcg ttcaccgccg tcgtccgcgc gagccccagc aggaagaacg tctggttggc
      421 gacgatcccg aggatgccaa ggccgcagag ccggaggagg tccatccggt ccgtcacccg
      481 cgccgcgccg gacgtccggt ggaccaccag gagcacgcac gcggcgccgg tcactcgtgc
      541 caggacgagg accaccgggg ggacctcgcc cagcacgtac ttccccgcga tggcgaggct
      601 ggagaagagg acctggacgg cgatgagcgc gagatggacc aggatgggca tcgtcgaacg
      661 ataatccgcc ctgtcgcagc gccgtatcgg tgaggcgtgc gcggcggcgg atgagctcta
      721 tcggtggcgc cgccgggggt ggggacgtcc tcgttgcctg cggccctcgg atcgggcgcc
      781 gccttcgcgg agcagctcgc gcagggcgcc cagcgtgtcg gcccggagca gcggatggtt
      841 gccgcgcgcc accgcaaatc ggacaggccg gtcgaggacg ggcacctccc ggtcctggag
      901 ccggcgcgcc tgcccctccc gcaggcgcag ccgcgcgaca ccgccgacct ggggctccac
      961 ctcgtactcg atcggaacgg catcgccgag cacgcgggcc gaggacggga gggccaggag
     1021 acgttcccga acctcgggat cgacgcgctc cgccgggtcg agcgaaatac gccgcgccag
     1081 gaactcttcc cagctcgcga cctcgacgac ctgttcccgg agcgccgatc ggacggcgtc
     1141 gggaccggcc gccgcgagcg tgccgcccga ccgtcgccac aactcgtcga gctccagcag
     1201 cgcccggcga atgcctccct ggcgcggatg caccgtctct cccgcgacca gggcctcgac
     1261 tagcgcctcg ccggcggccg cacggtgttc cgcggggacg gcgccccgga tcggctcgag
     1321 ggagtcctcg aggtcgaacc cgaagtaggt gagcttgtgg gtgagcgcga ggtgctgatg
     1381 cttgcgcggg cccgtcacca ccacccgggc ccgctcccat tgggcgtgcg cccgcacctc
     1441 gtcgtacgac agggtggtcc cctcgatcga cgcgcgcggc gtgccgaagc ggtcgccgaa
     1501 gtaccgcagg ttgccggcca ccgcgcccca gttccccgcc accgacgtct tcgacacgcg
     1561 cgcctcctga ccgtcggcgg tgcgctcgtc gatcacgagg tcgaagggca tcactcgcgc
     1621 caccagctca ttccagcggc gctgcaggtc gtggccggcg tacggcaacc gcggcggcag
     1681 ctcctgttcc atcgcccggt agatcgatgc cgcgccgagc gcgatgtcct cgatggcctt
     1741 gaccaggaca ccccgccgct ccgcccaggc ttcgagcccc tcctcgaaga ggtggcgggg
     1801 gagcccgtac acctgtccca catagccgtg ctggttgacc gcctccgcga acaggttgta
     1861 cgccgtcagg tggtcgctcc cgcggaccac gacgccgtgc aggtcgcgtt cttcgcgggt
     1921 gatccggtgc aggctgtcga tgttggcgca gacggccacg atcgggagca cctccgggtc
     1981 ggcgtgcacc agaagctccc cccacgcgcg gtcgacgggc atcgcctcga cctgccggcc
     2041 gtagggcgtg agcttcccct cggccatcag cccgcgcgag gtgagggtcc gcaccgcgcg
     2101 cacgtaggcc gcccggtcga gcgggaccgg caggtcgagg tcgcgggcgt cgacgcccag
     2161 ggcggcgcag gtcacggcca cccgctccgc gtcgccggcc agctggaact cgggcggcgt
     2221 cggccgcaac tcctcgaaga cgaggtcgcg gtcgctcagg atgaccacct cgccccccgg
     2281 cacgcggccg tgcacccgcc cggccatctg gaggatctcg ttggcgccga ggtacatccg
     2341 gtgcagcacg ttgcggccgc ggtccacgac gttgccgtag cgcgcgtcgt agatgatgac
     2401 ggtgtcgagg ccgcggatgt tcagcgccga ctgtcccgcc gcggtcatcg cgagcaggaa
     2461 gggacgctcc acctcgccgt cgaggaacgg gcggatgacc cggatcggct cgcccccgtg
     2521 gtagaaggcc gccgacagct tcggcc
//
LOCUS       DASUAP010000089         4226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1228780, whole genome shotgun sequence.
ACCESSION   DASUAP010000089 DASUAP010000000
VERSION     DASUAP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228780"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            540..2060
                     /locus_tag="VFU45_02200"
     CDS             540..2060
                     /locus_tag="VFU45_02200"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5357904.1"
                     /translation="MLSLDGRPGNAVWWADGVIREVGPSSRLDLSVPRGVPVWDLPGA
                     IITPGMVDGHTHFSLWALGRRRVQLAGCAEREEAVRRVGAALPVDGWVRGQGWNSNGW
                     REAPDRSVLDRVHSGPVFLESLDVHAAWVNSAALRAAGIDRDTPDPYGGRIVRDAAGE
                     PTGLLLERAVELMLPHLPPVPPGQLTTAVTEGIQEAHRLGITGVHDVEGHAAWVVWRA
                     LALAGELPLRVLFHPPVESLGQLVAEGWRSGVRAGPLTLGGVKLFLDGSLGSRTAWML
                     EPFEGTHDRGMPITDGGLARAAVEMASAAGISATVHAIGDAAVRRALDLLESVPKAGI
                     PHRIEHFQCVQPVDLARAARAGILVSMQPAHLLTDAPLVDRHWGDRGAFAYHFRSILA
                     AGNQVVFGSDAPVASLDPRDGIFAALDRRPAAGGGAGWRADERIGLEAAVRAYTRAAA
                     AAAGAPDVGALAPGMAADLVAWEVDPAAERSDSGSAFRAGQAVLTVLAGRPVWDAT"
     gene            2149..3603
                     /gene="pyk"
                     /locus_tag="VFU45_02205"
     CDS             2149..3603
                     /gene="pyk"
                     /locus_tag="VFU45_02205"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU5357905.1"
                     /translation="MSPHKARFTKIVATLGPAWSSPDRMHALLDGGVNVVRINASHGT
                     PAIREQWISDLQRVLAERKDAAAILLDVQGPRIRVGDLAEPITLAAGQEVVFAPEAVA
                     RPGEIPTTYDDLATDVKVAARILLDDGLLSVEVTGISGVRVRGVVRYGGLLKSHKGMN
                     LPGIQVSAPSLTDKDREDLAMAGRLGVDYVALSFVRRREDMEELRKLLPRSVKTVAKI
                     EKDTALLNLEAIIDASDAIMVARGDLGVELPFEEVPLVQKRIIREANLRGKPVITATQ
                     MLESMVHAPRPTRAEASDVANAILDGTDAVMLSAETAVGDFPLEAVQAMDRIAREMEA
                     NRRGRSPAFDLAVGRRGPGAEAATGKPVEPVRQEDAIGVATVAAAELLKAPLVVCFTV
                     SGFTARKVAAYRPAQPIFALTTDRGTYRQLCLVWGVVTGLADHLPSYDEMLSVARQQI
                     LERHLAAPGDKLVVTAGVPFDVPGTTNLLKIEAV"
     gene            3767..>4226
                     /locus_tag="VFU45_02210"
     CDS             3767..>4226
                     /locus_tag="VFU45_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5357906.1"
                     /translation="MRLTFLGTGTSFGVPQVGCDCAVCRSTDPRDRRSRSAALVASGG
                     ATILIDTPPELRLQLLAARPPTIDAVLYTHEHADHIAAIDDLRIYSVRAGRPVSVYGP
                     AETLDRLRSSYRYIFDDAVQPPAGTSKPRLSLVPLEAGVEVTVAGLPVLPL"
BASE COUNT          575 a         1525 c         1494 g          632 t
ORIGIN      
        1 ggggcaggga cgccaccccc tccgactctc gccactcgcc gcccttccct cacccgccac
       61 ccgtgactcg ccacccggtc caccacccgg cggggcaggg aggccaccct ctccgactcc
      121 cgccactcgt ccccgcattc cggaatgcgg aaaacccatg cgcccagccg acaggggcac
      181 tgttatgcac gtcatttgca tgaatattca acttcggcgg acactgtcgc ggtccaactt
      241 gtccacatcc agcgaacgcg tttacccatt gtgcaaacac gacttacgaa atccacctca
      301 cgtggataaa cccgccgtga tcaagtacgt tcaacggcaa tcggttccaa acctttgatt
      361 cacattttca cgacctccac aagtggaaaa ccctccttct ccacagttgt gcgcagcggc
      421 tcgttgccag gtatcttggc cctcacccca gtccggagat gctggtgtgg ataactgacg
      481 cggccctgcg cgctgcccct cccctgcccc aggggagtgg gtatgcgcgt gctcgtcgag
      541 tgctttcgct cgatgggcgt ccagggaacg ccgtctggtg ggccgatggg gtcatccggg
      601 aggtggggcc gagctcaagg ctcgacctgt cggtgccccg gggggtgccg gtgtgggacc
      661 ttccaggggc catcatcacc ccggggatgg tcgatggtca tacccatttc tcgctctggg
      721 cgctggggcg gcggcgcgtc cagctggcgg ggtgcgccga gcgggaggag gcggtgcgga
      781 gggtcggcgc ggcccttccg gtggacggct gggtcagggg ccaggggtgg aactcgaatg
      841 gctggcggga ggcgcccgac cgatcggtcc tggatcgggt ccacagtggc ccggtcttcc
      901 tcgaatccct tgacgtgcac gccgcatggg tcaattcggc ggcgctccga gcggcgggca
      961 tcgaccggga cactccagat ccgtacggcg gtcggatcgt ccgggatgcg gcgggcgagc
     1021 ccaccggcct cctcctcgag cgggcagtgg aactgatgct tccccatctc cctcccgtgc
     1081 ccccggggca gctcacgacc gccgtgacgg agggcattca ggaagcccac cggctgggga
     1141 tcaccggtgt acacgacgtc gaagggcacg ccgcctgggt ggtgtggcgg gcgctcgccc
     1201 tggccggcga gctgccgctc cgggtgctgt ttcatcctcc cgtggagtcg ctgggccagc
     1261 tcgtggcgga ggggtggcgg agcggggtcc gggccgggcc gctgacgctg ggtggggtca
     1321 agctctttct cgacggaagc ctcgggagcc ggacggcgtg gatgctggag cccttcgaag
     1381 ggacccacga ccggggaatg ccgatcaccg atggcggcct ggcgcgggcg gccgtggaga
     1441 tggcgagcgc ggcgggcatc tcggccaccg tccacgccat cggcgatgcc gccgtccgcc
     1501 gcgcgctcga cctgctggag tccgttccca aggcggggat cccgcaccgg atcgagcact
     1561 tccagtgcgt ccagccggtg gaccttgccc gcgcggcgcg cgcggggatc ctggtgtcga
     1621 tgcagccggc gcacctgctc accgacgccc cgctcgttga ccgtcattgg ggggaccgcg
     1681 gcgccttcgc gtatcacttc cggagcatcc tggcagccgg gaaccaggtg gtcttcggca
     1741 gcgatgcgcc ggtcgcctcg ctggacccac gggacgggat cttcgcggca ctcgaccgtc
     1801 gtcccgccgc cggcggcggc gccgggtggc gcgcggacga gcggatcggt ctcgaggcgg
     1861 cggtgcgcgc ctatacccgg gccgccgcgg ccgcggcagg cgcgccggac gtcggcgccc
     1921 tggcgcccgg gatggccgcg gacctggtgg cgtgggaggt cgaccccgcc gccgagcgga
     1981 gcgacagcgg gagtgccttc cgggccggac aggcggtcct cacggtgctc gccggccggc
     2041 cggtctggga cgcgacctga cctgccccgg ggtccggcgt cctaccgccg cccggcgccg
     2101 gcgctatcct tagcaggact cttcctcttt catccgaccg gaatgaacgt gtcaccccac
     2161 aaggcccgct tcaccaagat cgtcgccacg ctgggtcccg cgtggagctc ccccgaccgc
     2221 atgcacgcgc tcctcgacgg cggcgtcaac gtggtccgga tcaacgcctc gcacggcacc
     2281 cccgccatcc gcgagcagtg gatctccgat ctccaacggg tgctcgcgga gcggaaggac
     2341 gccgccgcca tcctgctcga cgtccagggg ccgcgcatcc gcgtgggcga cctggccgag
     2401 cccatcaccc tcgctgccgg ccaggaggtg gtcttcgcac cggaggcggt cgcgcggccg
     2461 ggggagatcc ccaccaccta cgacgacctc gccaccgacg tgaaggtcgc ggcgcggatc
     2521 ctcctcgacg atgggctcct ctcggtggaa gtgaccggga tctccggtgt ccgggtgcgg
     2581 ggcgtggtcc ggtacggcgg cctcctcaag tcgcacaagg ggatgaacct gcccgggatc
     2641 caggtgagcg cgccctcgct cacggacaag gaccgcgagg acctcgcgat ggcggggcgc
     2701 ctgggggtgg actatgtggc gctctcgttc gtgcgccggc gggaggacat ggaggagctg
     2761 cgcaagctcc tgccccggtc ggtcaagacg gtggccaaga tcgagaagga caccgccctg
     2821 ctcaacctcg aggcgatcat cgacgcgtcc gacgcgatca tggtggcccg cggcgacctc
     2881 ggggtcgagc tgccgttcga ggaggtcccc ctggtccaga agcggatcat ccgcgaggcc
     2941 aaccttcggg ggaagccggt catcaccgcg acccagatgc tggagtcgat ggtccacgcg
     3001 ccgcgcccca cccgggccga ggcctccgac gtggccaacg cgatcctcga cgggaccgac
     3061 gcggtgatgc tctccgctga gaccgccgtg ggcgacttcc cgctcgaggc ggtgcaggcg
     3121 atggaccgca tcgcgcgcga gatggaggcc aaccggcggg gccggagccc ggccttcgac
     3181 ctcgccgtgg ggcggcgcgg gcccggcgcc gaagccgcca ccgggaagcc cgtcgagccg
     3241 gtgcggcagg aggatgcgat cggcgtcgcc acggtcgccg cggcggagct cctcaaggcg
     3301 ccgctggtcg tctgcttcac ggtgagcggg ttcaccgcgc gcaaggtggc cgcgtaccgc
     3361 ccggcgcagc cgatcttcgc gctcaccacg gaccggggca cctatcgcca gctctgcctg
     3421 gtgtggggcg tcgtgacggg gctcgccgat cacctgccga gctacgacga gatgctctcg
     3481 gtcgcgcgac agcagatcct cgagcgccac ctggcggcgc cgggcgacaa gctggtcgtc
     3541 accgccggcg tccccttcga cgtcccgggc accaccaacc tgctcaagat cgaggcggtg
     3601 tgacgcaggt ggcggtgaac cggccgcgag cgacgggcgg ggagcggggc gcgcggcgcg
     3661 atcccggatc cctgaggcac gacgccccgg gcccggtcgc gccgttccgc gccacccgcc
     3721 acccgccacg tacccgcggc ccttcactcg tccctcggca cttaccgtgc gcctgacctt
     3781 cctcggcacc ggcacctcgt tcggcgtgcc gcaggtggga tgcgactgtg cggtgtgccg
     3841 gtcgacggat ccgcgcgacc ggcgctcccg gtctgccgcg ctcgtggctt ccggcggcgc
     3901 caccatcctg atcgacactc cgcccgaact ccgccttcag ctcctcgccg cccggccccc
     3961 gacgatcgac gcggtgctct acacccacga gcacgccgat cacattgccg cgatcgacga
     4021 cctgcggatc tattcggtcc gggcaggacg gccggtgtcg gtctacggtc cggcggagac
     4081 gctggaccgg ctccgctcga gctatcgcta catcttcgac gacgcggtgc agccgcccgc
     4141 ggggacgtcg aagccgcgac tttccctggt gccgctcgag gcgggggtcg aggtgacggt
     4201 cgcgggcctg ccggtgcttc cgctcg
//
LOCUS       DASUAP010000090         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1351555, whole genome shotgun sequence.
ACCESSION   DASUAP010000090 DASUAP010000000
VERSION     DASUAP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351555"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFU45_02215"
     CDS             <1..281
                     /locus_tag="VFU45_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357907.1"
                     /translation="MMRDERLKFVPDPFRAALRQMTALAGGRYALIPAAGVFFHDPAN
                     GETAELLLVLADSREATVVWRTRAVGHGATARAALDDALDHVLPAMVR"
     gene            281..1297
                     /locus_tag="VFU45_02220"
     CDS             281..1297
                     /locus_tag="VFU45_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEU5357908.1"
                     /translation="MAFDVTLIPGDGIGPEITAATVKVLERSGLKFRWDEQLAGMAGV
                     DATGTPLPDATLESIRKTGLALKGPLTTPVGTGFRSVNVGLRKEFELFANVRPAVTLV
                     PGQRYSGIDITLIRENLEGLYVGVEHFVQVGDDPKAVAQSAAIVTRLECERIARYAFE
                     YAVHHHRKKVTIVHKANILKMTSGLFLETAKTVAREYAGRVESNDLIIDNTAMQLVLR
                     PEQFDVIVTTNMFGDILSDEISGLVGGLGLAPGANIGMRAAIFEAVHGSAPDIAGKGI
                     ANPSAQMLAAAMLLDHIGEIARGDRLRAAIKRVIVDDNVRTRDLGGTASTAEFGDAVA
                     RRMA"
     gene            1272..1562
                     /locus_tag="VFU45_02225"
     CDS             1272..1562
                     /locus_tag="VFU45_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357909.1"
                     /translation="MPSPVGWPELPCARCGTRTAGIDFGALCPDCTAALKAQAQKLGR
                     RAALAATVAVALWGIFAVSRAPLARTYAAAAVIATYLIVRRVVERIALEVLK"
     gene            1559..>2721
                     /locus_tag="VFU45_02230"
     CDS             1559..>2721
                     /locus_tag="VFU45_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357910.1"
                     /translation="MKTAFRIVALLLLGAPLTAHLSPLTAQTSATIYNDGRVLVRRTI
                     AAAVPRGVSIQHLPLGQLDPGSIFSLDDGVSIQGVRYDGGTDDASLLRRAVGRTLVFR
                     SGTMLKDTASATVVSIAPFRLRLADGSIVTQSIGVPSYPAEAAFLAPSLDVTVSSDAA
                     RRDLRLGYFAPGGGGWQASYQAVFSGGQARLSGAAVIGAGQVALDSAEIQLLAGQVSV
                     ASGGGRGPMKAMNGPMAMEAVARDAGTVEQKVGEFHIYTLPGRLTVAPGVITTAALFD
                     PASAPYQKTYVVAGALPWWGGFGQDMTTTDVPVQVTYVVKRPRKTDLGDRPLPGGIVR
                     MFEPDSAGRLQLIGETNIDHTPAGEDLELNAGTAFDLTARRVQTSWQTGRDSS"
BASE COUNT          393 a          976 c          968 g          384 t
ORIGIN      
        1 ggatgatgcg cgacgagcgg ctcaagttcg tgcccgatcc cttccgcgcg gcgctccgcc
       61 agatgacggc gctcgcgggg gggcgctacg cgctgatccc cgccgcgggg gtgttcttcc
      121 acgaccccgc caacggtgag accgccgagc tgctgctcgt gctggccgac tcgcgcgagg
      181 cgaccgtggt gtggcgcacc cgcgcggtgg ggcatggcgc cacggcccgc gccgccctcg
      241 acgacgcgct cgaccacgtg ctcccggcga tggtgcgctg atggccttcg acgtgaccct
      301 catcccgggc gacgggatcg ggccggagat caccgcggcg acggtcaagg tgctggagcg
      361 ctcggggctc aagttccggt gggatgagca gctggccggg atggcgggcg tcgacgccac
      421 cggtactccg ctccccgatg ccaccctcga gtccatccgg aagaccggcc tggcgctcaa
      481 gggacccctc acgacgccgg tggggaccgg gttccgctcg gtcaacgtgg ggctccggaa
      541 ggagttcgag ctcttcgcca acgtgcgacc ggcggtgacg ctggtgcccg gccagcgcta
      601 cagcggcatc gacatcacgc tcatccggga gaacctcgaa gggctctacg tcggcgtcga
      661 gcacttcgtg caggtgggcg acgatccgaa ggccgtggcc cagtcggcgg cgatcgtgac
      721 ccgcctcgag tgcgagcgga tcgcgcgcta cgccttcgag tacgcggtgc accaccaccg
      781 gaagaaggtc acgatcgtcc acaaggccaa catcctcaag atgacgagcg gcctcttcct
      841 cgagaccgcg aagacggtgg cgcgcgagta cgccgggcgg gtcgagtcga acgacctgat
      901 catcgacaac acggcgatgc agctggtgct ccgtcccgaa cagttcgacg tgatcgtgac
      961 caccaacatg ttcggcgaca tcctgagcga cgagatctcc ggcctggtgg gcggactggg
     1021 gctggcgccg ggggccaaca tcgggatgcg ggcggccatc ttcgaggcgg tgcacggcag
     1081 cgcgcccgac atcgcgggga aggggatcgc caacccgtcg gcgcagatgc tcgcggcggc
     1141 gatgctcctc gaccacatcg gcgagatcgc ccggggcgac cgactccggg cggcgatcaa
     1201 gcgcgtcatc gtggacgaca acgtgcgcac ccgcgacctg ggcggcacgg cctccacggc
     1261 ggagttcggc gatgccgtcg cccgtcggat ggcctgagct cccctgcgcc cgctgcggga
     1321 cgcgcaccgc ggggatcgac ttcggggcgc tctgccccga ctgcacggcg gcgctcaagg
     1381 cacaggcgca gaagctgggg cgccgtgccg ccctcgcggc cacggtggcg gtggcgctct
     1441 ggggcatctt cgccgtctcc cgcgcgccgc tcgcccggac ctacgccgcc gcggcggtga
     1501 tcgccaccta cctgatcgtg cgccgcgtgg tcgaacgcat cgcgctggag gtgctcaagt
     1561 gaagaccgca ttccgcatcg ttgcgctgct gctgctcggc gccccgctca ccgctcacct
     1621 ctcaccgctc accgcccaga cgagcgccac catctacaac gatggccgcg tccttgtgcg
     1681 ccgcaccatc gcggcggcgg tcccgcgcgg cgtttcgatc cagcacctgc ccctcggcca
     1741 gctcgacccg gggtcgatct tctccctcga cgacggcgtc agcatccagg gcgtccgcta
     1801 cgacggcggc accgacgatg cctcgctcct gcggcgcgcc gtcggccgga cgctggtctt
     1861 ccgctccggc acgatgctca aggacaccgc ctccgccacg gtggtgagca tcgcgccctt
     1921 ccggctgcgg ctggcggacg gctcgatcgt gacccagtcg atcggggtgc cgagctatcc
     1981 ggccgaggcg gcgttcctgg cgccatcgct cgacgtgacg gtcagcagtg acgcggcccg
     2041 gcgggacctc cgcctcggct acttcgcgcc tggcgggggc gggtggcagg ccagctacca
     2101 ggcggtgttc agcggcggac aggcacggct ctcgggtgcc gcggtgatcg gcgcgggcca
     2161 ggtggcgctc gacagcgccg agatccagct gctggcgggg caggtgagcg tggcgagcgg
     2221 cggcggccgc ggcccgatga aggcgatgaa cggcccgatg gcgatggaag ctgtcgcgcg
     2281 cgatgcgggc acggtggaac agaaggtggg ggagttccac atctacaccc tgccgggccg
     2341 gctcacggtg gctcccggcg tcatcacgac cgcggcgctc ttcgatcccg caagcgctcc
     2401 ctaccagaag acctacgtgg tggccggcgc gctcccctgg tggggtgggt tcgggcagga
     2461 catgaccacg acggacgttc cggtccaggt gacgtacgtc gtcaagcggc cccgcaagac
     2521 cgatctcggc gaccgtccgc tccccggcgg aatcgtcagg atgttcgagc ccgacagcgc
     2581 cggccggctc cagctcatcg gcgagaccaa catcgaccac acccctgccg gcgaggacct
     2641 ggagctcaac gccggcacgg ccttcgatct cacggcccgg cgggtgcaga ccagctggca
     2701 gacggggcgc gattcctccc a
//
LOCUS       DASUAP010000091         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1228849, whole genome shotgun sequence.
ACCESSION   DASUAP010000091 DASUAP010000000
VERSION     DASUAP010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228849"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFU45_02235"
     CDS             complement(<1..680)
                     /locus_tag="VFU45_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEU5357911.1"
                     /translation="MPNAPQVVFLSGVRTGFGAFGGTLKDLSATDLGVVVAKAAIARA
                     GLPVERYDHAVVGNALQTSKDAIYLARHVALRAGLPIPVPALTVNRLCGSGFESIIEG
                     ARQILLGESRVVLAGGTESMSQAPHVLRGARWGYRLGPTPPLEDSLWEALRDTECDKS
                     MAETAEALAEKYGITRADVDAYALRSQQAAEAAWQAGAFADEVVPVTIRDRKTKQDLA
                     WALDEHRRP"
     gene            complement(758..1447)
                     /locus_tag="VFU45_02240"
     CDS             complement(758..1447)
                     /locus_tag="VFU45_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5357912.1"
                     /translation="MSDLELLYRRLVDSLIAVDPARLHRPLQLAELYQTVLPYRAARR
                     ALNLDSSEDYDNLLLRLCAGEGGYVTIEPEEAQIRFQAELASPSPDLGILREYAAAEL
                     TLSTERLAYALGPGPEAAYAPPEPEEELEPELPPHRPQPTGQVPWTVDAPEPAGGIIP
                     LREGAPSRERDGLHRCSYCGGALPSGRQVNFCPHCGQSQSFTRCPECSAEVELGWHHC
                     INCGHPVGDET"
     gene            1522..2370
                     /locus_tag="VFU45_02245"
     CDS             1522..2370
                     /locus_tag="VFU45_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357913.1"
                     /translation="MRITWLALAAITWTAPLAAQTDCTLGHLPYNSNEARLLRKFEAP
                     LVFAPGGAPAELHGGQVQFGVDVTYLPHIDSTTATPTYCRPGKPPENVNLLSAIPRPR
                     MAIGLSPALVLELSWIPPIRLNGVKADIFGVALARRWALGGVRHLTARLHGTVGQIDA
                     TITCTEAQIQPSGPPQCAGLPAPSDDRFQPNNVGVDAALDWRLHGGGLRPYVGAGYTV
                     ELPRFRVDPNNTLVNQRVEGNYTRPSLFGGLTWMAGPALGVSGEVYAVPGDLVTGRVA
                     VRWSGK"
     gene            complement(2685..>3140)
                     /locus_tag="VFU45_02250"
     CDS             complement(2685..>3140)
                     /locus_tag="VFU45_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HEU5357914.1"
                     /translation="LSGAIAVLQLLAQKEKRLRLLLISDSEYLVKGVREWAPGWMARG
                     WTRKGGPIENLELWQALMKSVPLHDAQWTWVRGHSGHPKNEYVNDLAVRAATRQETSA
                     GVVESGFDAWLAGQRAKKRYLDYDPDAAFTTLEARLAAGERFAVAEGGK"
BASE COUNT          473 a         1094 c         1097 g          476 t
ORIGIN      
        1 ggccggcggt gctcatcgag cgcccaggcg aggtcctgct tcgtcttccg gtcgcggatc
       61 gtcaccggca ccacctcgtc ggcgaaggca cccgcttgcc acgccgcctc cgccgcctgc
      121 tggctccgga gcgcgtaggc gtcgacgtca gcgcgggtga tcccgtactt ctccgccaac
      181 gcctcggcgg tctccgccat cgacttgtcg cactcggtgt cgcggagtgc ctcccagagg
      241 gagtcttcga gcggcggcgt cgggcccagg cggtaccccc aacgcgcccc gcgcagcacg
      301 tgcggcgcct ggctcatcga ttcggtgccg cccgccagca ccacccggct ctcgccgagg
      361 aggatctggc gggcgccctc gatgatcgac tcgaagccgg aaccgcagag ccggttgacc
      421 gtgagggcgg ggacggggat gggaagcccg gcccggagcg cgacgtggcg ggccaggtag
      481 atcgcgtcct tggaggtctg gagcgcgttg ccgacgaccg cgtggtcgta ccgctcgacc
      541 gggaggccgg cgcgggcgat cgcagccttc gcgaccacga cccccaggtc ggtggcggag
      601 aggtccttga gcgtgccgcc gaacgcgccg aagccggtgc gtacaccgga gaggaacacg
      661 acttgagggg cgttcggcat agggttggaa tatgcccccg ccgggcgccc ccctcaagca
      721 acggacccgc agctcagatg gatgcggcgt tccggcgcta tgtctcgtct ccgacaggat
      781 gcccgcagtt gatgcagtga tgccagccca gttccacctc cgcgctgcac tcggggcagc
      841 gggtgaatga ctggctctgc ccgcagtggg ggcagaagtt gacctgccgc cccgacggca
      901 gggcaccgcc gcagtaactg cagcgatgga gcccgtcccg ttcccgcgac ggcgccccct
      961 ccctgagcgg gatgatccct ccggccggct ccggcgcgtc cacggtccat gggacctggc
     1021 ccgtcggctg gggccggtgc ggggggagct cgggttccag ctcctcctcg ggctccggtg
     1081 gcgcgtaggc cgcctcgggc cccggtccca gcgcgtatgc cagccgttcg gtcgagagcg
     1141 tgagctccgc cgcggcatat tcccggagga tgccgaggtc ggggctcggg ctcgccagct
     1201 ccgcctggaa ccttatctgg gcctcttcgg gttctatagt gacgtacccg ccctcgccgg
     1261 cgcagagccg gagcagcaag ttgtcgtagt cttcgctgga atcgaggttg agcgcccgcc
     1321 gggccgcccg gtacgggagg acggtctggt acagctccgc gagctggagc ggacggtgca
     1381 gccgggccgg gtcgacggcg atcaggctgt ccaccagccg gcgatacagg agttcaaggt
     1441 cgctcaaggc gtgctcggga tgcggggatg ggacgtctga ggctacggat gcggtgttac
     1501 cctgtcaagc aaggagccct gatgcgcatc acctggctgg ccctggcggc gatcacctgg
     1561 acggcgccgc tcgcggccca gaccgactgc accctggggc acctgccgta caattccaat
     1621 gaagctcggc tgctccggaa gttcgaggcg ccgctcgtct tcgcgcccgg cggcgcgccg
     1681 gctgaactcc atggcgggca ggtgcagttc ggcgtcgacg tcacctacct cccgcacatc
     1741 gactcgacca ccgccacgcc gacctactgc cggccgggga agccgccgga gaacgtgaac
     1801 ctcctcagcg ccattcctcg cccgcgcatg gccatcggtc tctcgccggc cctggtgctg
     1861 gagctgagct ggatcccgcc gatccgcctg aacggcgtca aggccgacat cttcggtgtc
     1921 gccctcgccc gccgctgggc gctgggcggc gtccggcacc tcaccgcgcg gctgcacggc
     1981 accgtcggcc agatcgacgc caccatcacc tgcaccgagg cgcagatcca gccctcgggc
     2041 ccgccgcaat gtgccgggct cccggcaccc tcggatgacc gcttccagcc caacaacgtc
     2101 ggggtcgatg cggcgctcga ctggcgcctg cacggcggcg gactccgtcc ctacgtcggc
     2161 gccggctaca ccgtcgagct tccccgcttc cgcgtggatc cgaacaacac gctcgtcaac
     2221 cagcgggtgg aagggaacta cacccgcccc tcgctcttcg gcggcctcac ctggatggcg
     2281 ggtccggcac tcggcgtgag cggcgaggtc tacgcggtcc cgggggacct ggtcacaggg
     2341 cgggtggcgg tgaggtggag tggcaagtaa gaagtaagaa gtgaaaagtg aaaagggaaa
     2401 agggaaggaa gaggagaagg agaaggagag ggagggagaa ggagaaggaa agagggacga
     2461 tggaacaggt gagagagaag aggggtggat cgcgtacggg cgagggagtc atcctgaggg
     2521 cgcgcagcgg ccgaaggatc tctcgggtgg acgcgcgccg atccttcgct gcgctcagga
     2581 tgacgttcgg tggtcaggcg tcgggtcgcc gcaggcgacc cccacagacg ggaacgatgc
     2641 ccctcttccc gctccccttt gcacttttca ctcttcactc ttcactactt gccgccctcc
     2701 gccaccgcaa atcgctcccc cgccgcgagc cgggcctcga gcgtggtgaa cgcggcatcc
     2761 gggtcgtagt cgaggtagcg cttcttcgcc cgctggcctg cgagccaggc atcgaagccc
     2821 gactcaacga cgccggccga ggtctcctgc cgggtggcgg cgcggaccgc gaggtcgttg
     2881 acgtattcgt tcttggggtg gcccgagtgg ccccgcaccc aggtccactg cgcgtcgtgg
     2941 agcggcacgg acttcatcag tgcctgccag agctccaggt tctcgatcgg gccccccttc
     3001 cgcgtccatc cccgcgccat ccagccgggc gcccactccc gcactccctt gacgaggtat
     3061 tccgagtccg agatgaggag cagccgcagg cgcttctcct tctgcgccag cagctgcagc
     3121 acggcgatgg cgccggagag
//
LOCUS       DASUAP010000092         8948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1011002, whole genome shotgun sequence.
ACCESSION   DASUAP010000092 DASUAP010000000
VERSION     DASUAP010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8948
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011002"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1204
                     /gene="sucC"
                     /locus_tag="VFU45_02255"
     CDS             65..1204
                     /gene="sucC"
                     /locus_tag="VFU45_02255"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU5357915.1"
                     /translation="MNLHEYQARALLQRAGVPMFEGDVAATPEEAAGIAKKLGGKVVV
                     KAQVHAGGRGKAGGVKLAATPGEAKEVAARILGMTIKGLTVHKVLVVPAADIASEAYV
                     GIIMDRESQRPVFMVSPAGGIDIEEVAAKTPEKILKLPVDPRFGLLPHQALTLGFFLY
                     KDFGHVKAAAAILQQLYRAFTENGASLAEINPLVTTPDGKVLALDAKVSIDDNELDRR
                     PDLAALRDETAEAPSEVEARKANLTFIKLDGNVGCVVNGAGLAMATMDLVKYYGGEPA
                     NFLDIGGSSNPEKVVNALRIITSDPNVKGILFNIFGGITRTDDVANGLKTALEKFPVK
                     VPIVVRLTGTNEAEAVKILRGIGMEALSDMDEAVARVVKLVKEAA"
     gene            1201..2073
                     /gene="sucD"
                     /locus_tag="VFU45_02260"
     CDS             1201..2073
                     /gene="sucD"
                     /locus_tag="VFU45_02260"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU5357916.1"
                     /translation="MSVFIGKDTRLVVQGITGRDGSFHARQMIAYGTKVVAGVTPGKG
                     GQTFEGTVPVFDTVAEAVARAGANTSVIYVPPAGAAGAIYEAVDAGIKLVVCITEGVP
                     VMDMTRVMPYVREHGARLIGPNCPGAITPGEAKVGIIPGNICAPGRVGLVSRSGTLTY
                     EVVNHLTKNGIGQSTCVGIGGDPIIGTNFIDCLRAFQDDPRTDAIVMMGEIGGTDEQH
                     AAEFVKQHVTKPVVGFIAGQTAPPGRRMGHAGAIISGHAGTAAEKMAAFEAAGISVMK
                     RPVDVVPLLKARMK"
     gene            2070..2975
                     /locus_tag="VFU45_02265"
     CDS             2070..2975
                     /locus_tag="VFU45_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5357917.1"
                     /translation="MRLTGLLAPERVLVPLRGRSLLEAVDELLAVSAAGQGVRDPAQV
                     AGVRREARLEDVVSLGPHAFLPHFRTEAVDRVVAALGVAATPLPWPDDPAVAARIVLL
                     ILAPPRDAARYLQALAAFSRALADPDVVGALHRAAGAAQALAVPGMADVELEGPLLVR
                     DIMTPDALSVSPDAPLAEAARVLVQHRWEALPVVGPGGEVVGLLSNRELLQHLVPAYM
                     QRVITGEFRAVPRAPAHAARRPLDIPVRDVMARSVLCVSEDQAVTDVASLMANKQIDR
                     CPVVKDGRLTGLLTRADIVRKLLGS"
     gene            3004..3438
                     /gene="ndk"
                     /locus_tag="VFU45_02270"
     CDS             3004..3438
                     /gene="ndk"
                     /locus_tag="VFU45_02270"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU5357918.1"
                     /translation="MSNTQITLAIVKPDAVATHKIGAIIAHLEREGFAVRSARMVRLT
                     KAQAGAFYAVHQGRPFYPELVEFMTSGPCVPLALERADAVAHLRTVIGATDPREAAAG
                     TIRKLHAESKGRNAIHASDSAENAAREVGFFFSEGELAGLSA"
     gene            3553..4017
                     /locus_tag="VFU45_02275"
     CDS             3553..4017
                     /locus_tag="VFU45_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU5357919.1"
                     /translation="MLQVDLRDLQKGPVDTVERVPPDDELFAGLDLGLATPLQVEGRL
                     QATGEGDVFWTGRLAGTVRGHCRRCLADVTFPVATDVSAYFSDDPEAADDPEVYVLAP
                     RATQVDLRPVVREEVVLAVPPFLLCRDDCAGLCPKCGADLNQGPCGCAAPKA"
     gene            4030..4212
                     /gene="rpmF"
                     /locus_tag="VFU45_02280"
     CDS             4030..4212
                     /gene="rpmF"
                     /locus_tag="VFU45_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU5357920.1"
                     /translation="MAVPKRKMSKSRKRLRRGHHSGAGMKTQACPRCSSPKLPHRVCP
                     TCGYYRGKKAVEVGDE"
     gene            4215..5228
                     /gene="plsX"
                     /locus_tag="VFU45_02285"
     CDS             4215..5228
                     /gene="plsX"
                     /locus_tag="VFU45_02285"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU5357921.1"
                     /translation="MIRVALDGMGGDHAPAAEVEGMALALAELPATFRVQLVGRPEVL
                     EPELAKHPEIDRSRLDVIAAPDVIGMDEKPLQAVRKKPNSSLVVGLALQKAGRSDAYV
                     SAGNTGAVLAASTLMLGLHEGVKRASVATPFPTADGAVLVLDGGANVDCTAAELVGFA
                     RLGTVYMRDILGRAEPAVGLLNVGEEDEKGNAAVREAHGLLKQAAGLRYIGNIEGRDI
                     LVGHAKWGQIDVVVCDGFVGNVVLKFYESVARLIVRLLKRDAPAILERDDIQKAFRTL
                     DYSEYGGAPLLGVKGVSIIGHGSSGPTAIKNAIRVAVQSVESNLSRHIGEEFARREAT
                     ASA"
     gene            5225..6214
                     /locus_tag="VFU45_02290"
     CDS             5225..6214
                     /locus_tag="VFU45_02290"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU5357922.1"
                     /translation="MIRRPIAFIAGLGTAVPPRVVTNRDFERTLDTSDQWIVERTGIR
                     ERHHADPGMTVAELARQASVIALRQAGLGPDDIGTIVLSTATPDRLLPSTACDLQALL
                     GAKRAAAFDISAACPGFLYGLTLAEGLIASEQSENVLVVGAEMLTSITDMTDRSTAIL
                     FGDAAGAAVVRKATGDRGLLSTFIRADGTLAELLYRPAGGAAHPFDAQVLADRSFLMK
                     MAGREVFKAAVLTMAEACDEALKRAGVKAEEIDLMVPHQANVRIIEATAKHAGMPMDK
                     VMVNLDRYGNTSSASIPLALVEAEAAGRIKPGSLLLLVAFGAGFTWASTVVRW"
     gene            6211..7137
                     /gene="fabD"
                     /locus_tag="VFU45_02295"
     CDS             6211..7137
                     /gene="fabD"
                     /locus_tag="VFU45_02295"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU5357923.1"
                     /translation="MTVILCPGQGAQKVGMGKDLAERFPAARDTFRAIDEALGVALSR
                     IMWEGPEDELTRTHNAQPAILAHTAAVFAVVRSRLGAVEVALGHSLGEYSAYITAGAL
                     TPEAAAKLVRRRGELMFEAGTRRAGTMAAVLGLGADQVAAACAEASTGGEVAVAANLN
                     EPTQTVISGDPAAVTRAGEGCKARGAKRVIGLKVSGAFHSPLMAPAVPGLRDALAAAG
                     FREPRFPVVANASAGLVTTAADAVRLLAEQLTAPVRWVECQQAAARSAPGAAYVELGP
                     GTVLTGLLKKIVPGAVSTPLGSADDVEKFLNQ"
     gene            7146..7889
                     /gene="fabG"
                     /locus_tag="VFU45_02300"
     CDS             7146..7889
                     /gene="fabG"
                     /locus_tag="VFU45_02300"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU5357924.1"
                     /translation="MPGPLDLSGKTAFVTGSTRGIGRAVAAALHGAGAKVAILGRDAA
                     RAQAVAAEIGERARGFACDVGDTASIQAAVDAVTRELGPVDILVNNAGITRDNLLLRM
                     GEDEWDQVLDANLKGAFRTVKAVLKGMMKRGGGRIINITSIVGLTGNKGQANYAASKA
                     GLVGFTKSVAKEYASRNVLANCVAPGFIETDMTAALPPDARAALLKEIALERLGRPED
                     VAGAVLFLASDLAAYVTGQVLVVDGGMVI"
     gene            7924..8166
                     /locus_tag="VFU45_02305"
     CDS             7924..8166
                     /locus_tag="VFU45_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5357925.1"
                     /translation="MSSNVEDKVKDIIVEELGVEREKLTNEASFMEDLGADSLDTVEL
                     VMAFEKEFDIDIPDEDAEKLRTVGDALKYLHEKIGK"
     gene            8173..>8948
                     /locus_tag="VFU45_02310"
     CDS             8173..>8948
                     /locus_tag="VFU45_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEU5357926.1"
                     /translation="MHRVVVTGLGLVTPVGNTTEDAWAGLLAGRSGAGPITKFDTARQ
                     GVKFACEVKGFDALQYIDKKEARRYDLFAQFALGAAHQAVTQAGLEGKFPDPERTGVI
                     IGSGIGGMQTYEDNCAAFVLKGPDRVSPFFVPMFIPDIAAGLVSIRYGLKGPNFATVS
                     ACASSAHAIGESYRLIQHGVADAMVAGGSEAAITGLTVAAFNNMKALSNRNDAPEQAS
                     RPFDKDRDGFVLGDGAGVVVLESLEHATARGATILGEVIG"
BASE COUNT         1379 a         3094 c         3203 g         1272 t
ORIGIN      
        1 cgattatcag aaggactggc ggccctcgag ggccggaccg gaaccaccat cgtgaggacg
       61 tgacgtgaac ctgcacgagt accaggcgcg cgccctgctg cagcgggccg gcgtgccgat
      121 gttcgagggt gatgtcgccg cgacgccgga ggaggccgcc ggcatcgcga agaagctggg
      181 cgggaaggtc gtcgtgaagg cccaggtgca tgcgggcgga cgcggcaagg ccggcggggt
      241 caagctggcc gcgacgcctg gggaggccaa ggaagtggcc gccaggatcc tcgggatgac
      301 gatcaagggg ctcaccgtcc acaaggtgct cgtggtgccg gcggccgaca tcgcctcgga
      361 ggcctacgtc ggcatcatca tggaccggga gtcgcagcgc ccggtgttca tggtaagccc
      421 ggccggcggc atcgacatcg aggaggtcgc cgccaagacg ccggagaaga tcctgaagct
      481 tccggtcgat ccgcgcttcg ggctgctgcc gcaccaggcg ctgaccctgg gcttcttcct
      541 ctacaaggat ttcggccacg tgaaggcggc ggcggccatc ctccagcagc tctaccgggc
      601 gttcaccgag aacggcgcgt cgctcgccga gatcaatccg ctcgtcacga ccccggacgg
      661 caaggtcctg gcgctcgacg ccaaggtgag catcgacgac aacgagctcg atcgtcggcc
      721 cgacctcgcc gccctgcgcg acgagaccgc ggaggcgccg agcgaggtcg aggcccggaa
      781 ggccaacctc accttcatca agctcgacgg caacgtgggg tgcgtcgtca acggtgccgg
      841 cctggccatg gcgacgatgg acctggtcaa gtattacggc ggcgagcccg ccaacttcct
      901 cgacatcggc ggcagctcga acccggagaa ggtcgtcaat gcgctccgga tcatcacgag
      961 cgacccgaac gtcaagggca tcctcttcaa catcttcggc ggcatcaccc ggaccgatga
     1021 cgtggccaac gggctcaaga ccgcgctcga gaagttcccg gtgaaggtgc cgatcgtcgt
     1081 gcggctcacc ggcaccaacg aggcggaggc ggtcaagatc ctccggggca tcggcatgga
     1141 ggcactgagc gacatggacg aggccgtcgc gcgggtggtc aagctggtca aggaggccgc
     1201 gtgagcgtct tcatcgggaa ggacacgcgg ctggtggtgc aggggatcac cgggcgggac
     1261 gggtcattcc acgccaggca gatgatcgcc tacggcacca aggtcgtcgc cggcgtcacg
     1321 ccggggaagg gcggccagac gttcgagggc accgtccccg tcttcgacac cgtggcggag
     1381 gcggtcgcgc gcgccggggc caacacctcg gtcatctacg tgccgccggc cggcgcggcc
     1441 ggggccatct acgaagcggt cgatgccggc atcaagctgg tcgtgtgcat caccgagggc
     1501 gtgccggtga tggacatgac ccgggtgatg ccctacgtgc gcgagcacgg cgcgcggctc
     1561 atcggaccca attgccccgg cgccatcacc ccgggcgagg ccaaggtcgg catcatcccg
     1621 gggaacatct gcgcccccgg ccgggtcggc ctggtgtccc ggagcggcac gctgacctac
     1681 gaggtggtca accacctcac gaagaacggg atcggccaga gcacctgcgt cggcatcggg
     1741 ggcgacccga tcatcgggac gaacttcatc gactgcctgc gggcattcca ggacgacccg
     1801 cgcaccgacg ccatcgtgat gatgggagag atcggcggca ccgacgaaca gcatgcggcg
     1861 gagttcgtga agcagcacgt caccaagccc gtcgtcggct tcatcgccgg acagaccgca
     1921 ccgcccggac ggcggatggg ccacgccggc gcgatcattt ccggccacgc ggggaccgcg
     1981 gcggagaaga tggcggcgtt cgaggcggcg ggcatcagcg tgatgaagcg cccggtcgac
     2041 gtcgtcccgt tgctgaaggc ccggatgaag tgaggctcac cggcctcctg gcgccggagc
     2101 gggtgctggt cccgctccgc ggccgcagcc tcctggaagc ggtggacgag cttctcgcgg
     2161 tcagcgccgc agggcaaggg gtcagggatc cggcgcaggt cgccggggtg cggcgggagg
     2221 cgcggctcga ggacgtggtg tcgctcggcc cgcacgcctt cctgccgcat ttccgcaccg
     2281 aggcggtcga tcgggtggtg gcggcgctcg gcgtggccgc aactccgctc ccgtggccgg
     2341 acgaccccgc cgtcgccgcg cggatcgtgc tcctgatcct ggcgccgccc cgtgacgccg
     2401 cgcgatatct gcaggccctg gcggccttca gccgcgccct ggccgatccc gacgtggtcg
     2461 gcgccttgca tcgggccgcc ggtgccgccc aggccctggc ggtgccgggg atggcggacg
     2521 tggagctcga ggggccgctc ctggtgcgcg acatcatgac gcccgacgcg ctgagcgttt
     2581 cccccgacgc gccgttggcc gaggcggcgc gggtgctggt gcagcaccgc tgggaggcgc
     2641 tgccggtggt cggccccggg ggagaggtcg tcgggctgct gagcaaccgg gagctcctgc
     2701 agcacctggt gccggcctac atgcagcgcg tgatcacggg ggagttccgc gccgttcccc
     2761 gcgcgcccgc ccacgccgcc cggcggccgc tcgacatccc ggtgcgggac gtcatggcgc
     2821 gcagcgtgct gtgcgtctcg gaggaccagg ccgtcaccga cgtcgcgtcg ttgatggcca
     2881 acaagcagat cgaccgctgc ccggtggtga aggacgggcg gctgacgggc ctgctcaccc
     2941 gcgcggacat cgtgcggaag ttgctgggca gctgaacgac cattccacct cacggagtac
     3001 cgcgtgtcga acacccagat caccctcgcc atcgtcaagc cggacgccgt cgccacgcac
     3061 aagatcggcg ccatcatcgc ccacctcgag cgggagggct tcgcagtccg ctcggcccgg
     3121 atggtccgcc tcaccaaggc ccaggcgggc gcgttctacg cggtccacca gggccgcccg
     3181 ttctaccctg agctggtcga gttcatgacg agcggtccgt gcgtgccgct ggcgctggag
     3241 cgcgccgatg ccgtggccca cctccggacg gtgatcggtg cgaccgaccc ccgcgaggcg
     3301 gcggccggga cgatccgcaa gctccatgcg gagtcgaagg gccgcaatgc catccatgcg
     3361 tccgacagcg cggagaacgc tgcacgggag gtcgggttct tcttctccga aggggaactg
     3421 gcggggcttt ccgcctgacc acgggcgggc cgggccggac ccggccgcgg cccggtgggc
     3481 ccgtccgttc cgggtccctc ggatccctcg tcggcttgac ctaaacccgc gccccggata
     3541 ggtttagtgg ctatgttgca ggtcgatctg cgtgatctcc agaagggacc ggtcgacacc
     3601 gtcgaacgcg ttccgccgga cgatgaactc tttgccgggc tcgatctcgg cctggcgacg
     3661 ccgctccagg tggagggccg cctccaggcg accggtgaag gcgacgtctt ctggaccggg
     3721 cggttggcgg ggacggtacg tgggcactgt cgccggtgcc tcgccgacgt gacattcccg
     3781 gtcgcgaccg atgtgtccgc gtatttcagc gacgacccgg aggccgccga cgatccggag
     3841 gtctatgtcc tcgcgccgcg ggccacccag gtcgacctgc gccccgtcgt ccgtgaggaa
     3901 gtggtgctgg cggtgccgcc gttcctgctc tgtcgggacg actgcgccgg gctctgcccg
     3961 aagtgtggtg ccgacttgaa ccaggggccg tgcgggtgcg cggccccgaa ggcgtgatcc
     4021 gaggatccca tggccgtacc caagagaaag atgtccaagt cccgcaagcg gctccggcgc
     4081 gggcaccatt cgggcgcagg gatgaagacc caggcgtgcc cgcgttgctc cagccccaag
     4141 ttgccgcacc gcgtctgtcc gacgtgcggc tattaccggg gcaagaaggc ggtcgaagtc
     4201 ggggacgagt gagcgtgatc cgcgtcgcgc tggacgggat gggtggcgat cacgcccccg
     4261 ccgccgaggt cgagggcatg gccctggccc tggcggaatt gccggccacg ttccgcgtgc
     4321 agctcgtcgg gcgcccggag gtcctggagc ccgagctcgc caagcaccct gagatcgacc
     4381 gttcgcgcct ggacgtcatc gcggccccgg atgtgatcgg gatggacgag aagccgctcc
     4441 aggccgtccg caagaagccg aattccagcc tcgtcgtcgg gctcgccctc cagaaggcgg
     4501 gcaggtccga cgcgtatgtg tccgccggca acaccggggc ggtcctcgcg gcctccacgc
     4561 tgatgctcgg cctccacgag ggcgtgaaac gcgcctcggt cgcgaccccg ttcccgaccg
     4621 ccgacggcgc ggtgctggtc ctcgatggcg gggccaacgt cgactgcacg gccgccgagc
     4681 tcgtcgggtt cgcccgcctc ggaacggtct acatgagaga tatcctcggc cgtgccgaac
     4741 ccgccgtcgg gctcctcaac gtcggcgagg aggacgagaa agggaacgcc gccgtccgcg
     4801 aagcccacgg gctcctcaag caggcggcgg gcctccgcta catcggcaac atcgaagggc
     4861 gggacatcct cgtcgggcac gccaagtggg gccagatcga cgtggtcgtc tgcgacggct
     4921 tcgtgggcaa tgtcgtgctc aagttctacg agtcggtggc ccggctcatc gtgcggctgc
     4981 tcaagcgcga tgcgcccgcc atcctggagc gcgacgacat ccagaaggcg ttccggaccc
     5041 tcgattactc cgagtacggc ggggcgccgc tcctcggcgt gaagggcgtg tcgatcatcg
     5101 ggcacgggtc ctccggcccc accgcgatca agaacgccat ccgggtcgcg gtccagtcgg
     5161 tcgagtcgaa cctgagccgc cacatcggcg aggaattcgc ccggcgcgaa gcgacggcct
     5221 cggcatgatc cgtcgcccga tcgcgttcat cgcgggcctc ggcaccgccg tgcccccgcg
     5281 cgtcgtcacc aaccgcgact tcgagcggac cctcgatacc tcggaccagt ggatcgtcga
     5341 gcggaccggc atccgcgagc gccatcacgc cgaccccggg atgaccgtcg ccgaactcgc
     5401 ccgccaggcg agtgtgatcg cgctgcgcca ggccggcctc ggccccgacg acatcggcac
     5461 catcgtcctg tcgaccgcca cccccgaccg gctcctcccc tcgacggcgt gcgacctgca
     5521 ggccctgctc ggggccaagc gggcggcggc attcgacatc tccgccgcct gcccggggtt
     5581 cctttacggg ctcaccctgg ccgaggggct cattgcgtcg gagcagagcg agaacgtgct
     5641 ggtcgtcggc gcggagatgc tcaccagcat caccgacatg accgaccgat cgaccgccat
     5701 cctcttcggc gatgcggcgg gcgcggcggt ggtgcgcaag gccaccggcg atcgcgggct
     5761 gctgtcgacc ttcatccggg ccgatggaac gctcgccgag ctgctgtacc gcccggccgg
     5821 gggcgcggcc catccgttcg atgcccaggt gctcgccgac cgcagcttcc tgatgaagat
     5881 ggccggccgc gaagtgttca aggccgccgt gctcacgatg gccgaagcgt gcgacgaggc
     5941 cctcaagcgg gccggcgtca aggcggagga gatcgacctg atggtgccgc accaggccaa
     6001 cgtgcgcatc atcgaggcca ctgccaagca cgccgggatg ccgatggaca aggtgatggt
     6061 gaacctcgac cgctacggca acacatcctc ggcgtccatc ccgctggcgc tggtcgaggc
     6121 ggaggccgcc gggcgcatca agccggggtc gctgctcctc ctcgttgcgt tcggcgcagg
     6181 attcacctgg gccagcacgg tggtgcgctg gtgacagtga tcctctgccc cggccagggc
     6241 gcgcagaagg tcgggatggg gaaggacctc gccgagcgct ttccggccgc cagggacacc
     6301 ttccgggcca tcgacgaggc actcggcgtc gccctctcca ggatcatgtg ggaaggcccg
     6361 gaggacgagc tcacccggac ccacaacgcc cagcccgcta tcctcgccca tacggccgcg
     6421 gtcttcgccg tggtgcggtc acggctgggg gcggtagagg tggcgctggg ccacagcctc
     6481 ggcgagtaca gcgcgtacat caccgccggc gcgctcacac cggaggccgc cgcgaagctc
     6541 gtccggcgcc gcggtgaact gatgttcgag gcagggacgc ggcgcgccgg cacgatggcc
     6601 gcggtgctcg gcctcggcgc cgaccaggtg gccgcggcct gcgccgaggc gtcgaccggg
     6661 ggcgaagtcg cggtggccgc caacttgaac gagccgaccc agaccgtcat ctccggcgac
     6721 cccgccgccg tgacccgcgc cggcgagggc tgcaaggccc ggggtgccaa gcgggtcatc
     6781 gggctcaagg tgagcggggc tttccactct ccgctgatgg cgccggccgt cccggggctc
     6841 cgggatgcgc tggccgctgc cgggttccgg gaaccccgat tcccggtcgt ggccaacgcc
     6901 agcgcggggc tggtcaccac cgcggccgac gccgtccggc tcctcgccga gcagctcacg
     6961 gcgccggtgc gctgggtcga gtgccagcaa gccgccgctc gatcggcgcc cggcgcggcc
     7021 tacgtcgagc tcgggcccgg caccgtgctc accggcctcc tcaagaagat cgttcccggc
     7081 gccgtgagca cgccgctcgg cagcgccgat gacgtcgaga agttcctgaa tcagtgagtg
     7141 tcccgatgcc tggtccgctc gacctctccg gcaagaccgc gttcgtcacc ggcagcaccc
     7201 ggggcatcgg gcgcgcggtg gcggccgcgc tgcacggggc gggggccaag gtcgcgatcc
     7261 tcggccggga cgcggcccga gcccaggccg tcgccgccga gatcggcgag cgtgcgcggg
     7321 gcttcgcctg cgacgtcggc gataccgcgt cgatccaggc cgcggtcgac gccgtcaccc
     7381 gggaactcgg tccggtggac atcctggtga acaacgcggg catcacccgc gacaacctgc
     7441 tcctgcggat gggcgaggac gagtgggacc aggtgctcga cgccaacctc aagggcgcgt
     7501 tccgcaccgt caaggccgtg ctcaagggga tgatgaagcg cgggggcggg cggatcatca
     7561 acatcacgag catcgtgggg ctgaccggca acaagggcca ggccaactac gcggccagca
     7621 aggcggggct ggtcgggttc accaagtcgg tggccaagga gtacgccagc cggaacgtcc
     7681 tggccaactg tgtggccccg ggcttcatcg agaccgacat gaccgcggcc ttgccgcccg
     7741 acgcgcgggc cgcattattg aaggagatcg cgctggaacg gctgggccgg ccggaggacg
     7801 tggcgggcgc cgtgctgttc ctggcgtcgg atcttgccgc ctacgtcacg gggcaggtcc
     7861 tggtggtcga tggtgggatg gtcatctagg gtcggatacc ttttcaagcc tcttgaggag
     7921 cccatgagca gcaacgtcga ggacaaggtc aaggatatca tcgtcgagga gctcggagtc
     7981 gagcgcgaga agctcaccaa cgaggcgagc ttcatggagg acctgggcgc cgacagcctc
     8041 gataccgtcg agctcgtcat ggcgttcgag aaggaattcg acatcgacat ccccgacgag
     8101 gacgcggaga agctccgcac cgtgggcgat gcgctgaagt acctccacga gaagatcggg
     8161 aagtaggcct tggtgcaccg cgtcgtcgtc accgggctcg gcctggtgac cccggtcggc
     8221 aacaccacgg aggacgcctg ggccggcctg ctggcgggcc ggtccggggc gggacccatc
     8281 accaagttcg acaccgcccg tcagggcgtg aagttcgcct gcgaggtgaa gggcttcgat
     8341 gcgctccagt acatcgacaa gaaggaagcg cgccggtacg acctgttcgc ccagttcgcg
     8401 ctgggggcgg cccaccaggc cgtgacccag gccggactcg aggggaagtt ccccgatccg
     8461 gaacggacgg gcgtcatcat cggcagcggc atcggcggga tgcagaccta cgaggacaac
     8521 tgcgcggcgt tcgtcctcaa ggggcccgat cgggtgtcgc ccttcttcgt gccgatgttc
     8581 atccccgaca tcgccgcggg gctcgtgtcg atccgctacg ggctcaaggg ccccaatttc
     8641 gccaccgtct cggcctgcgc gtcctcggcg cacgccatcg gcgagtcgta ccggctcatc
     8701 cagcatggcg tggccgacgc gatggtggcc gggggctccg aggccgccat caccgggctc
     8761 accgtggccg ccttcaacaa catgaaggcg ctgtccaacc ggaacgacgc cccggagcag
     8821 gccagccgcc cgttcgacaa ggaccgcgac ggtttcgtgc tcggcgacgg cgccggcgtg
     8881 gtcgtgctgg agtcgctgga acacgccacc gcgcgcggcg ccaccatcct gggcgaggtc
     8941 atcggtta
//
LOCUS       DASUAP010000093         4984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_629364, whole genome shotgun sequence.
ACCESSION   DASUAP010000093 DASUAP010000000
VERSION     DASUAP010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629364"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /gene="priA"
                     /locus_tag="VFU45_02315"
     CDS             <1..1761
                     /gene="priA"
                     /locus_tag="VFU45_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU5357927.1"
                     /translation="ATTRHLEEQLGFGAGVIKALVTRGLARVDRVERIRDPFADEAGT
                     PPPADLTDDQSRALAILGALPPAGGALLFGVTGSGKTLVYLEAVRRALAEGKGAIILV
                     PEIGLTPQTVRRVRGAFGDQVAVLHSALSDGERADAWRLLRRGERRVAVGARSALFAP
                     IPALGVIVVDEEHEASYKNGEAPRYHARDVAAVRARLEGARLILGSATPAVETWARAS
                     AGKLTRVALPARIGDRPLPPVEVVDLRTAPRVKDTGAIPWSAALDAAVDRALARKEQV
                     LLLLNRRGFASFLQCEACGAVRDCPNCSISLTVHRTPERLACHYCAHAEPMPKACGEC
                     GSPLQRMKGIGTQQLESYLGQRYPGARLARMDLDTTSTKWGHHRILGAVERGEVDILF
                     GTQMIAKGLDFPNVTLVGVVDADTGLHLPDFRASERTFQLLAQVAGRAGRGPKGGRVL
                     VQTRQPGAPAVRFAVTHDVEGFLAAELASRESPPYPPSLGLANVLVSGVNDVAVGNAA
                     AAVADWCQALVEAQKLPLTVLGPAPAPLARIKDRWRWHLLVKGAPEEIGRFVRYAAPR
                     WREAGHGVRVIIDRDPVSLL"
     gene            1821..2906
                     /locus_tag="VFU45_02320"
     CDS             1821..2906
                     /locus_tag="VFU45_02320"
                     /inference="COORDINATES: protein motif:HMM:NF044507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357928.1"
                     /translation="MATYKLPDFTRPPLATAPEATFAPLPSDGVLPEGFFSTSNLPTY
                     VKVGGKWLAPSRPRMDGVIVRRGDAIETTEPRSLKRGDQVAMGMAEDGSEGIFVHTDG
                     FLAGAHGANEFRFMSTEVSRERPVNYEELASRIGEEARGGGYLIWVAGPALVHSRARA
                     DFEWFIRNGYVQAVMAGNAVAVHDMEAAIYGTTLGMTNTGEATEGGHGLHMRAINRIR
                     AAGSIERAVEQGIVTSGIMHALVTRKVPYVLAGSIRDDGPLPGVYSDTLAAQDAMREH
                     TSRATGAVLVATALHAIAVGNMLPAFHMRGGVPTPLMTICVDQTEFVVNKLKDRGTHQ
                     AYGVVTNAQDFMHVLRSYVEKWERTRG"
     gene            2885..3301
                     /locus_tag="VFU45_02325"
     CDS             2885..3301
                     /locus_tag="VFU45_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357929.1"
                     /translation="MGTYAGLTPAGGPDARWHRLADEVAKAVPVAEIDGIWTFQPLKN
                     GPLEAGTAIVSRVDGERRRIYTGRYVATIKGKQRGQFQAVVEEVGSGPLDALDRLLAD
                     VEKRVDEGAPSPVPVVEWFPDLSAPLPAEADAAPER"
     gene            3282..3755
                     /locus_tag="VFU45_02330"
     CDS             3282..3755
                     /locus_tag="VFU45_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEU5357930.1"
                     /translation="MRLPNGEAEAQLERFRHWLRDHRLPVTRPRESVARAIFAADGHP
                     SADGLARALKAGGAPVGTATLYRTIDLLIESGLVRARDFGEGVRRFEPAAGADDHGHF
                     VCERCGAVTEFSTERLERMLPLMADEAGFRLERHDVALHGVCRDCVGRPAEAVRR"
     gene            3752..4480
                     /locus_tag="VFU45_02335"
     CDS             3752..4480
                     /locus_tag="VFU45_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5357931.1"
                     /translation="MINAPALSLAVALLAGLLSFLSPCVLPLVPSYLGFLTGMTAGEM
                     HDRRRYALLHALVFVLGFTLIFLLLGAGASALGRGLKLHHEWIERGGGALIIIFGVIT
                     LGVFNIPLLHLDTRVHLDRKPLGFLGSFLVGMAFGAGWTPCIGPVLGGILSLASVQDS
                     LSRGLLLLGAYSAGLAVPFLLAAWALEGFLEWFQRFRRWLPWVQRVSGALLILAGILL
                     VSGEFTRMSGWLQALTPSFLQRWL"
     gene            complement(4487..4864)
                     /locus_tag="VFU45_02340"
     CDS             complement(4487..4864)
                     /locus_tag="VFU45_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEU5357932.1"
                     /translation="MTAARVPARRGIDARMTTTAMGFDGYYVGEQYGVVRGVSVRSRS
                     IVGNFAAKLQQVFGGDITILTRLCESARQDAFEIMLAHAERLGANAVIGVRYDATEIM
                     AGVTEVLCYGTAVAVEPLPAEPA"
BASE COUNT          658 a         1743 c         1841 g          742 t
ORIGIN      
        1 gccaccaccc gccacctcga ggaacagctc ggcttcggcg cgggggtcat caaggcgctg
       61 gtgacccgcg gcctcgcccg ggtcgaccgg gtcgagcgga tccgggaccc gttcgccgac
      121 gaggcgggca cgccgccgcc ggccgacctc accgacgacc agtcgcgggc gctcgcgatc
      181 ctgggcgcgc tcccgcccgc cggcggggcg ctcctcttcg gcgtcaccgg gagcgggaag
      241 accctggtgt acctggaagc ggtgcggcgc gccctcgcgg aggggaaggg ggcgatcatc
      301 ctggtgccgg agatcggcct gacgccgcag acggtgcgtc gggtgcgggg cgccttcggc
      361 gaccaggtgg cggtgctcca cagcgcgctc tccgacggcg agcgggccga cgcctggcgc
      421 ctgctgcggc gcggcgaacg ccgggtggcg gtgggggcgc gctcggcgct ctttgcgccg
      481 atccccgccc tcggcgtcat cgtggtggac gaggagcacg aggcgagcta caagaacggc
      541 gaggcgccgc gctatcacgc gcgcgacgtg gcggccgtcc gcgcccggct cgaaggggcg
      601 cggctcatcc tgggaagcgc caccccggcg gtggaaacgt gggcccgggc atcggcgggg
      661 aagctcacgc gcgtcgccct ccccgcgcgg atcggcgacc gtccgctccc gccggtcgag
      721 gtggtggacc tccggaccgc gccgcgggtc aaggacaccg gtgccattcc ctggagtgcg
      781 gcgctcgatg cggccgtcga ccgtgccctg gcgcggaagg agcaggtgct gctcctcctc
      841 aatcggcgtg ggttcgcgtc gttcctgcag tgcgaggcgt gcggcgcggt gcgcgactgt
      901 cccaactgct cgatctcgct cacggtgcac cggacaccgg agcggctggc gtgccactac
      961 tgcgcccacg ccgagccgat gccgaaggcg tgcggcgaat gcgggagccc gctccagcgg
     1021 atgaagggga tcggcaccca gcagctcgag tcgtacctgg gccagcgata tcccggtgcc
     1081 cggctggcgc ggatggacct cgacacgacc agcaccaagt ggggccacca ccgcatcctg
     1141 ggggcggtgg agcgggggga ggtcgacatc ctcttcggga cccagatgat cgccaagggg
     1201 ctcgacttcc ccaacgtcac gctggtgggc gtggtggatg ccgacaccgg gctccacctc
     1261 cccgatttcc gggcgtcgga gcggaccttc cagctgctgg cccaggtggc cgggcgggcg
     1321 gggcggggcc ccaagggagg gcgggtcctg gtccagacca ggcagccggg ggctcccgct
     1381 gtccgctttg ccgtgaccca cgacgtggag gggttcctcg cggccgagct cgccagccgc
     1441 gaaagcccgc cgtatccgcc gtcactgggg ctcgccaacg tgctggtgag cggggtgaac
     1501 gacgtggcgg tggggaatgc ggcggcggcg gtggccgatt ggtgccaggc gctggtcgag
     1561 gcacaaaagc tccccctcac cgtgctcggc ccggcgccgg cgccgctcgc gcgcatcaag
     1621 gatcgctggc gctggcacct gctggtcaag ggggcgcccg aggagatcgg acggttcgtg
     1681 cgctacgcgg ccccccggtg gcgggaggcg ggacacgggg tccgggtgat catcgaccgc
     1741 gatcccgtct ccctgctctg acccactgac ccacgtccca ctgtcccgct gtcccgctcc
     1801 cccttacgtt tctccgcggt atggccacct ataaactccc cgatttcacc cgcccgcccc
     1861 ttgccacggc gccggaggcg acgttcgcgc cgctgccgtc cgacggcgtc ctgcccgaag
     1921 gcttcttctc cacctcgaac ctcccgacct acgtgaaagt gggcggaaag tggctggcgc
     1981 cgagccggcc gcggatggac ggtgtgatcg tccggcgggg ggatgcgatc gagaccaccg
     2041 aaccccggag cctcaagcgg ggcgaccaag tggcgatggg gatggccgag gacgggagcg
     2101 aggggatctt cgtccacacc gacgggttcc tggccggtgc gcacggggcg aacgagttcc
     2161 gcttcatgtc gaccgaggtg agccgggagc gtccggtcaa ctatgaggag ctggcctccc
     2221 ggatcggtga ggaagcgcgg ggcggcgggt atctcatctg ggtggcgggg ccggcgctgg
     2281 tgcattcgcg ggcccgggcg gatttcgagt ggttcatccg gaacgggtac gtccaggcgg
     2341 tgatggcggg gaacgccgtc gccgtgcacg acatggaggc ggccatctac gggaccacgc
     2401 tcggaatgac caacaccggc gaggcgaccg aaggtggcca cgggctccat atgcgggcga
     2461 tcaaccggat ccgtgccgcc gggagcatcg agcgggcggt ggagcagggg atcgtgacga
     2521 gcgggatcat gcacgcgctg gtgacccgga aggtccccta cgtcctggcc ggttcgatcc
     2581 gggacgacgg cccgctcccg ggtgtctaca gcgacacgct cgcggcccag gatgcgatgc
     2641 gggagcacac cagccgcgcc accggggcgg tgctcgtggc gacggcgctc cacgccatcg
     2701 cggtcgggaa catgctgccg gcgttccata tgcgcggcgg cgtcccgacg ccgctgatga
     2761 ccatctgcgt cgaccagaca gaattcgtgg tcaacaagct gaaggaccgg ggcacgcacc
     2821 aggcttacgg cgtcgtcacc aacgcgcagg acttcatgca cgtcctccgg agctacgtgg
     2881 aaaaatggga acgtacgcgg ggctgacccc cgccggcggc ccagacgccc gctggcaccg
     2941 gctggccgac gaggtcgcga aggcggtccc ggtggccgag atcgacggga tctggacctt
     3001 ccagcccctc aagaacgggc cgctggaggc cggcaccgcg atcgtgtcgc gggtggacgg
     3061 cgagcgccgc cggatctaca ccggccgcta cgtcgcgacg atcaagggga agcagcgcgg
     3121 ccagttccag gcggtggtcg aggaggtggg gagcggaccg ctcgacgcgc tcgatcggct
     3181 gctggccgat gtcgagaagc gggtggacga aggcgccccc tcgccggtgc cggtggtcga
     3241 gtggttcccc gacctctccg ctccgctccc cgccgaggcc gatgcggctc ccgaacggtg
     3301 aggccgaggc gcagctcgag cgcttccggc actggctccg cgaccatcgc ctcccggtga
     3361 cgcggccgcg cgaatcggtg gcccgggcca tcttcgcggc cgacggccac ccctccgccg
     3421 acggactggc ccgcgcgctc aaggccggcg gcgcgccggt cggcaccgcc accctctacc
     3481 gcacgatcga cctcctcatc gagagcggcc ttgtccgcgc gcgcgacttc ggcgagggcg
     3541 tccgccggtt cgagccggcc gccggcgccg acgaccacgg ccatttcgtc tgcgagcggt
     3601 gcggcgcggt cacggagttc tccaccgagc gcctggagcg gatgctcccg ctcatggccg
     3661 acgaggcggg gttccgcctg gagcgccatg atgtggccct ccacggcgtc tgccgcgact
     3721 gcgtgggccg ccccgccgag gcggtgcgcc ggtgatcaac gcgcccgcgc tgagcctcgc
     3781 ggtggccctg ctggcggggc tcctgtcgtt cctctccccc tgcgtcctcc ccctggtgcc
     3841 gagctacctc ggattcctca ccgggatgac ggcgggggag atgcacgacc gccgccgcta
     3901 cgccctgctg cacgcgctgg tcttcgtcct cgggttcacg ctcatcttcc tcctcctcgg
     3961 cgcgggggcg agcgcgctcg gccgcgggct caagctccac cacgaatgga tcgagcgcgg
     4021 gggcggcgcg ctcatcatca tcttcggcgt catcaccctc ggcgtgttca acatcccgct
     4081 gctccacctc gacacccggg tgcacctcga ccggaagccg ctgggcttcc tgggatcgtt
     4141 cctcgtgggg atggcgttcg gggcggggtg gacgccgtgc atcgggccgg tgctgggcgg
     4201 catcctgagc ctggcaagcg tgcaggactc gctgagccgg ggcctcctcc tgctcggcgc
     4261 ctattcggcg gggctggcgg tgccgttcct cctggccgcg tgggcgctgg aggggttcct
     4321 ggagtggttc cagcggttcc ggcggtggct gccctgggtg cagcgggtgt cgggggcgct
     4381 cctgatcctc gccgggatcc tgctcgtgtc cggggagttc acccggatga gcgggtggct
     4441 ccaggcgttg acgccctcct tcctccagcg ctggctctga cccggttcac gccggctcgg
     4501 ccgggagcgg ctccaccgcg accgccgtgc cgtagcaaag cacctcggtg acccccgcca
     4561 tgatctcggt ggcatcgtag cgcacgccga tgaccgcgtt ggcgcccagc cgctccgcgt
     4621 gggccagcat gatctcgaag gcgtcctgcc gggcgctttc gcagagccgg gtcaggatgg
     4681 tgatgtcgcc gccgaagacc tgctggagct tggcggcgaa gttgccgacg atcgagcgcg
     4741 atcggacgct caccccgcgc accaccccgt actgctcccc gacgtagtaa ccgtcgaacc
     4801 ccattgccgt cgtggtcatc cgggcgtcga tcccgcgccg ggcaggcacg cgtgctgcgg
     4861 tcatgtgttc tccagtggaa gggcgtagcg ctcatagtct ggcgcttagg gcttagcgct
     4921 cagggtttag ggcttagcgc ttagggctta gcgcttaggg cttagggctt agggcttaga
     4981 gctt
//
LOCUS       DASUAP010000094         4721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_670320, whole genome shotgun sequence.
ACCESSION   DASUAP010000094 DASUAP010000000
VERSION     DASUAP010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670320"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..1380
                     /locus_tag="VFU45_02345"
     CDS             334..1380
                     /locus_tag="VFU45_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357933.1"
                     /translation="MPRFIRALAALILFPALLWAQAEHPDSVDRPPSHGALNLGIGHT
                     GLSIGNSRDWNGLRINWQDHWDGTINGVNLTLGNAKHNASATVNGLAVGIGPYAGKLH
                     GVTVGILGAVADSEMWGVQLAGLGAVSNGDAYGITLSGLGTVADGRMVGLNASGLATV
                     ANGELIGLNLAGLATVGDGNVRGISIAGLAVVANGGIDGLAAAGLATVTDGDLRGAAI
                     GGLATVVNGSFGGIGVGGLATVVDGDARGILAGGLANVVNGSFKGIAVGGLANVIDNV
                     HGIEVTAGKAESWEMSGASIAGWNKVKGPARGLAIAVFNDVKELHGLEIGLLNRARNN
                     RGIAEWLPGVNFHK"
     gene            complement(1482..1964)
                     /locus_tag="VFU45_02350"
     CDS             complement(1482..1964)
                     /locus_tag="VFU45_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5357934.1"
                     /translation="MQNRTTVERQSDRELVVTRTVNGPARLVFKAWTTADLFRQWWIP
                     KSMPITVHSCEMDARTGGGYKLMLSQAGGAPMPFFGKYLEVTPHSRLVWTNEEGGENG
                     AVTTVTFEEKGGKTLVTMRDLYPSKQALDDTMGQMPPETMNETFDQLEALLGTLGTNS
                     "
     gene            complement(1975..2340)
                     /locus_tag="VFU45_02355"
     CDS             complement(1975..2340)
                     /locus_tag="VFU45_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5357935.1"
                     /translation="MVQLARARLDTSFAALSDATRRGVLERLGRSDASITDLADRFQM
                     TLTGMKKHVGVLERAGFVTTEKVGRVRTCRIGPRQLEDEMAWLERYRQLCEARFNELD
                     RVVEDLQRKEDDNERTHRK"
     gene            2452..3678
                     /locus_tag="VFU45_02360"
     CDS             2452..3678
                     /locus_tag="VFU45_02360"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5357936.1"
                     /translation="MSSLEAKFAKLGTEHAPGQEVRHGNDLPGAGLRGAPLAGVPVDF
                     SHGDVNDTAFPPAPGALDAFIAGERRGGAQAYTEYRGAADLRDQVARKLAAFTGRPIK
                     AAEELIVTPGTQGALFLAIGATVNAGDRVAIVRPDYFANRKLVAFLGADVVPVRLDYL
                     AHSDRAGLDLDQLEDAFKSGVRTFLLSNPNNPTGVIYSPEEIAGIVTLANRYGATVIV
                     DELYSRLIYSGQTYTHLCAAGIASDQVVTILGPSKTESLSGYRLGVAFGAPHLIERAE
                     RLQAIVSLRAPGYSQAVLRTWFDEPAGWLVERIRRHEAIRDDLIRVFRTVEGLSVRTP
                     GGGSYLFPRLPALDLSLPDFVRALRVQAGAVVTPGTEFDPEATDSIRLNFSQNHPAAV
                     RAVERLAKLVERYRRR"
     gene            complement(3842..4585)
                     /locus_tag="VFU45_02365"
     CDS             complement(3842..4585)
                     /locus_tag="VFU45_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357937.1"
                     /translation="MLDLIGSIVGMAAIAIDLVAFTSALGGTPWRRLALAAVVGGWVG
                     LANHLAATGQLAFSTSRIVPGIAGLFGVPLLAVGALALGSRKVRAALLAVPTPTLIAL
                     NALRVLGVLFLFLAADGRLSGPFPYSAAIGDIVTGALAIPLALSVARGQASARAIRNW
                     NLFGALDLIAAVGFGLTSANGSPIHLFHVGVGSQAMQYLPYALVPTVLVPFYLVTHAV
                     IAAQLAHTRVEPTPRGARAGWREAGTATT"
BASE COUNT          737 a         1597 c         1670 g          717 t
ORIGIN      
        1 ggcgagcacc agggcgaccg tcgaggcggt ggcgcggtcg cggggtggct cagcggcggt
       61 cgcagtggtc acgtgcagct ctcgaaggaa gagagtagga ctgggataat gtgggaccac
      121 ggagtgcacg gaggacacgg agaggcacgg aggaaagcca ttcagtgtgg tcgaactgcg
      181 gagatccgct tgctcgacca cgtcacccgc agttctccgt gagtctccga gcaactccgt
      241 gcactccgtg gtcccacaac atccccacct gaaaccgaag ccggggtgtt tcgtagtgga
      301 acgcgcaccc cgttcctata tataggagtt cccgtgcccc gcttcatccg cgcccttgcg
      361 gccctgatcc tcttccccgc cctcctctgg gcccaggccg agcaccccga ttccgtcgac
      421 cggccgccat cgcacggcgc cctcaacctc gggatcggcc acaccgggct cagtatcggc
      481 aacagccgcg actggaacgg cctccgcatc aactggcagg accactggga cggcacgatc
      541 aacggcgtca atctgacgct cggcaacgcc aagcacaatg cgagcgcgac ggtgaacggg
      601 ctggccgtgg ggatcgggcc ctacgccggc aagctccacg gagtcacggt cggcatcctc
      661 ggcgccgtgg ccgacagcga aatgtggggg gtccagctcg ccgggctcgg cgcggtctcc
      721 aacggcgacg cctacggcat cacgctgagc gggctcggca ccgtggccga cggcaggatg
      781 gtgggcctca acgcatcagg gctcgccacc gtggccaacg gggagctcat cgggctcaac
      841 ctcgccggcc tcgccaccgt gggcgatggg aacgtccgcg gcatctccat cgcagggctc
      901 gccgtggtgg cgaacggggg gatcgacgga ttggcggcgg ccgggctcgc gaccgtgacc
      961 gatggcgacc tccgcggcgc ggcgatcggc ggcctggcca cggtggtcaa cggcagtttc
     1021 ggcgggatcg gcgtcggcgg cctggccacg gtggtggatg gcgacgcccg ggggatcctc
     1081 gccggcgggc tcgcgaacgt ggtcaacggc agcttcaagg ggatcgcggt gggcggcctg
     1141 gccaacgtga tcgacaacgt gcacggcatc gaggtcaccg cgggcaaggc ggaatcgtgg
     1201 gagatgtcgg gcgccagcat cgcgggatgg aacaaggtca agggaccggc gcgtgggctc
     1261 gcgatcgccg tattcaacga cgtgaaagaa ctccacggcc tcgagatcgg gctcctgaac
     1321 cgggcacgga acaaccgggg gatcgccgag tggctgccgg gggtgaattt ccataagtag
     1381 ctatcagcta tcagctatca gtcattggct gatggtcatg ggctggcagg ggatgagccg
     1441 gatgagggcc gccgttctgc ggccccatcc ggtcatcagc gtcagctgtt cgtgccgagc
     1501 gtgccgagga gcgcctcgag ctggtcgaac gtctcgttca tcgtctcggg aggcatctgc
     1561 cccatggtgt catcgagcgc ctgcttcgag gggtagaggt cacgcatcgt gacgagggtc
     1621 ttgcccccct tttcctcgaa ggtcaccgtg gtgacggccc cgttctcgcc gccctcttcg
     1681 ttggtccata ccaggcgtga gtgcggggtc acctcgaggt acttgccgaa gaacggcatc
     1741 ggcgctccgc cagcctgact cagcatcagc ttgtacccgc cgccggtccg cgcatccatc
     1801 tcgcaggagt ggacggtgat gggcatcgac ttcgggatcc accattgccg gaagaggtcg
     1861 gccgtggtcc acgccttgaa caccaggcgc gccgggccgt tgacggtccg ggtgacgacc
     1921 agctcgcgat cggactgccg ttccaccgtg gtgcggttct gcatcggatg tgcctcactt
     1981 cctgtgtgtg cgttcattgt catcttcctt ccgctgtaag tcctcgacca cccggtccag
     2041 ctcgttgaag cgcgcctcgc agagctgccg gtaccgttca agccaggcca tctcatcctc
     2101 caattggcgg ggaccgatcc ggcaggtccg cacccgcccg accttctccg tggtgacgaa
     2161 cccggcccgc tccaggaccc cgacgtgctt cttcatcccc gtcagcgtca tctggaaccg
     2221 gtcggccagg tccgtgatgg aagcgtccga ccggccgagc cgctccagca cgccgcgacg
     2281 ggtggcgtcc gagagggcgg cgaacgaggt atccaggcgg gcccgcgcaa gctgaaccat
     2341 atggttcagt atagcgaaag gcctggcgac gcacaagggg gccgacctcg ttgccccctt
     2401 cccgcccgcc ggcgatagat ttccgcctcg cgccgttcac cccgaggcca gatgagttca
     2461 cttgaggcga agttcgccaa gctcggcacc gagcacgcgc cgggccagga ggtgcgccac
     2521 gggaacgatc tccccggcgc cgggctgcgc ggggcgccgc tggccggggt gcccgtggat
     2581 ttctcccacg gggacgtgaa cgacaccgcc tttcccccag cccccggcgc cctcgacgcg
     2641 ttcatcgccg gcgaaaggcg cggcggcgcg caggcgtaca cggaataccg cggcgctgcc
     2701 gacctccggg accaggtggc ccggaagctc gcggcgttca ccgggcgccc gatcaaggcg
     2761 gccgaggagc tcatcgtgac tcccggcacg cagggggcgc tgttcctggc gatcggcgcc
     2821 accgtgaacg ccggcgaccg cgtggcgatc gtgcgccccg actacttcgc caatcggaag
     2881 ctcgtggcct tcctcggcgc cgacgtggta ccggtgcggc tcgactacct ggcgcactcc
     2941 gaccgggcgg ggctcgacct ggaccagctg gaggacgcct tcaagtcggg ggtacggacc
     3001 ttcctcctgt cgaaccccaa caacccgacc ggggtcattt actcgccgga agagatcgcc
     3061 gggatcgtga cgctagcgaa caggtacggc gcgaccgtga tcgtggacga gctctattcg
     3121 cggctgatct attcggggca gacgtacacc catctctgcg ccgcggggat cgcgagcgac
     3181 caggtggtca ccatcctcgg cccgtccaag accgagtcgc tgagcggcta ccggctcggc
     3241 gtcgcgttcg gcgcgccgca cctcatcgaa cgggccgagc ggctgcaggc gatcgtgtcg
     3301 ctccgcgcgc cgggatactc ccaggcggtg ctccgcacct ggttcgacga accggccggg
     3361 tggctcgtcg aacgcatccg ccggcacgag gccatccggg atgatctgat cagggtgttc
     3421 cggacggtag aagggctctc ggtccggacg ccgggcgggg ggagctacct ctttccgcgc
     3481 ctcccggcgc tcgacctgtc gctgccggac ttcgtgcgcg cgctccgggt gcaggccggc
     3541 gccgtcgtca cgccgggtac tgagttcgat cccgaggcta cggacagcat ccggctcaac
     3601 ttctcgcaga atcacccggc cgcggtgcgg gcggtggaac ggctcgcgaa gctggtggag
     3661 cggtatcggc ggcggtgagg ccggatgagg ggcggtgccc tgccccgatg gtgcatacta
     3721 tggacagggt gggcggggcg acggtaagcg tcgtgtgctt gagatagcac cccgaagcgg
     3781 caccgctgat ccgcgcgcat actgctccct gaccgccgct gagcacgcac tccccactcc
     3841 atcaggtggt cgcggtgccg gcctcccgcc agccggcccg cgcgccccgg ggagtcggtt
     3901 cgacgcgcgt gtgggccagc tgtgccgcga tgaccgcgtg ggtcaccagg tagaacggca
     3961 cgaggaccgt cggcacgagt gcgtatggca ggtattgcat cgcctgcgac cccaccccga
     4021 cgtggaagag gtggatcggg ctcccgttcg cggatgtcag gccgaacccg accgcggcga
     4081 tgaggtcgag cgcgccgaac aggttccagt tccggatggc gcgtgcgctg gcctgcccgc
     4141 gcgcgacgct cagcgcgagc gggatggcga gcgcgccggt cacgatgtcg ccgatggcgg
     4201 cggagtacgg gaacgggccg ctcaagcggc cgtccgccgc caggaagagg aagagcacgc
     4261 cgagcacgcg cagggcgttg agggcgatga gcgtgggtgt ggggaccgcg aggagcgcgg
     4321 cgcgcacctt ccgcgagccg agcgccaggg cgcccaccgc gaggagcggc acgccgaaca
     4381 gccccgcgat gccggggacg atccgactcg tcgagaaggc gagctgcccg gtggcggcga
     4441 ggtgattggc aagcccgacc cacccgccga cgacggcggc cagcgccagc cggcgccacg
     4501 gggtgccgcc gagcgcggag gtgaaagcga cgaggtcgat ggcgatggcg gccatcccca
     4561 cgatcgatcc gatgaggtcc agcatgcgtt gctcccggtg cgttgttatt ggtgtatatg
     4621 catatatcgt ggcgcgcacc gtcagcgcgc cgcgagcttg tgcgcggcgg cgtgggacgc
     4681 ccgccgcagc gccgcggcct ccgtccggcc gacccgccct t
//
LOCUS       DASUAP010000095         5256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_942783, whole genome shotgun sequence.
ACCESSION   DASUAP010000095 DASUAP010000000
VERSION     DASUAP010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942783"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFU45_02370"
     CDS             complement(<1..309)
                     /locus_tag="VFU45_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357938.1"
                     /translation="MADAAPMPSGPVDLSPALEEAGLARRHGVLEADGVSLAEIARQV
                     GTPVYVYDAGVIGRRYRALDRVLEGVPHRICFAVKANSNLAVLRVLRELGAGADLVSG
                     G"
     gene            complement(309..1763)
                     /gene="proS"
                     /locus_tag="VFU45_02375"
     CDS             complement(309..1763)
                     /gene="proS"
                     /locus_tag="VFU45_02375"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU5357939.1"
                     /translation="MADDKSLTTRAQDFSAWYNELIARAELADYSPVRGCMVIRPNGY
                     AVWEQMQRALDQMFKDTGHQNAYFPLFIPQSFLSKEAEHVEGFAPETAVVTIGGGKEL
                     EEPLVVRPTSETIIYAMYAKWIQSYRDLPILINQWANVVRWEMRTRLFLRSTEFLWQE
                     GHTAHASEAEAEAETRQMLGVYRTFMEGWMAMPVLTGLKSDSERFAGALRTYACEALM
                     QDNKALQAGTSHNLGQNFARAFDVTFQTASGGIDHVWSTSWGVSTRLIGGLIMTHGDD
                     RGLVCPPLLAQYQAVIVPIYKTDLERASVLEAADRWRQALRGAGIRTHLDAREGMKPG
                     AKYYEWEARGVPFRLELGPRDVAAGHAMLARRTGGAKEPVPFDAAPARLHDEILRMQK
                     DLFEAARARREANSVRGATKAQLLETMSGEGGGGFVYAGWCGDAKCEAEIKAETKATI
                     RVLPDEEFRSPVAPATCVWCGRAATAEAVWAKAY"
     gene            complement(1879..3168)
                     /gene="hisS"
                     /locus_tag="VFU45_02380"
     CDS             complement(1879..3168)
                     /gene="hisS"
                     /locus_tag="VFU45_02380"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU5357940.1"
                     /translation="MPTTALPGFRDFYPEDFALRAHIFETWRRVAGRYGFEEYDGPPL
                     ESLDLYTEKSGEEIVGQLYEFTDKGDRRVALRPEMTPSLARMVAARANGLKKPIRWFS
                     IPQLFRYERQQRGRLREHFQLNCDLIGESTPLADAEIIALAIDVMRAFGLSARDVRVR
                     VSDRILITDWLRQAGVPDAALGVALQAIDRLEKSHDQARAMLEGVAPSSAAQIVRRLG
                     DPSARTELRQELVARGGTLSRFGAVLDALDDMGLADWVEFDPTIVRGLAYYTGTVFEL
                     FDTGRTLRAICGGGRYDALTGAIGGVELGAVGFGMGDVVLGELLKDKALVPAHAASID
                     VFLAAITEGDVPAVLRLAHELRDAGIRVEYALGVQAVGKQLKLADARNARFAVVIGPD
                     DREKHQVILKDLRAKGQMPVARGEIVQEVLKTIRPGR"
     gene            complement(3242..4462)
                     /locus_tag="VFU45_02385"
     CDS             complement(3242..4462)
                     /locus_tag="VFU45_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU5357941.1"
                     /translation="MPLSYPQALEFLFPRTTTIKFGLETTGLLLERLGRPQDQFPAIH
                     IGGTNGKGSVTTLVAAALAALGLRVGQYTSPHLVSFRERIRVDGVAIGEEAVAAWTGE
                     LRSPIEETGATFFEASTAIAFADFAARGADIAVVEVGLGGRLDSTNVITPLVAGVTKI
                     ARDHMKYLGESLEEIAREKAGIAKPGVPFVIGETDPALVDVLRREALARGGVPVAVPP
                     VERWTGALGLAGGHQRRNAAVARRVLEALPAALRPDEGAVAAGFAAARLPGRFDRRGK
                     WLFDVAHNPDGMRALVRALEEERLPRPIHAVVSILGDKEWAEMLVELDRAVDRGWLTI
                     APTADQRRWTLEWLERWLEDPVRPPARAAWHLEADFRRALREAEAGAGTVLVTGSFHT
                     VGDVMHELGMDADG"
     gene            4533..5096
                     /locus_tag="VFU45_02390"
     CDS             4533..5096
                     /locus_tag="VFU45_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEU5357942.1"
                     /translation="MPRHRTLPDPFVARLRQMPGYRELMTDYTRCSGCEKCERSIVYL
                     TPREQQHARHTGLRVYGQGAAARLNRAGCKCPFYAAETFQCTAYLERPLICNLFPMDL
                     LEREEDGRHWWVLFGACDEVRVGRLRGKVEAFRQLARRIDAEMPVELKRSLVADAAGA
                     IPEPAFYDHPVHWLVPMSGLGAVSGEQ"
BASE COUNT          739 a         1932 c         1818 g          767 t
ORIGIN      
        1 gccccccgac accaggtcgg cgccggcgcc gagctcgcgg agcacccgga gcaccgccag
       61 attgctgttg gccttgaccg caaagcagat ccggtgcggc acgccctcca gcacccggtc
      121 gagcgcccgg taccgccggc cgatgactcc cgcgtcgtac acatagaccg gcgtccccac
      181 ctggcgggcg atctccgcga gcgacacgcc gtccgcctcg agcacgccgt gccggcgcgc
      241 gagccccgcc tcctcgagcg cggggctcag gtcgacaggc ccggacggca tcggcgcggc
      301 gtccgccatc agtacgcctt ggcccagacc gcttcggcgg tggcggcgcg tccgcaccag
      361 acgcaggtgg caggtgccac cggcgaacgg aactcctcgt cggggaggac acggatcgtg
      421 gccttcgtct ccgccttgat ctccgcctcg cacttcgcat caccgcacca gccggcgtag
      481 acgaatccgc cgccgccctc ccccgacatc gtctcgagga gctgggcctt cgtggcgccg
      541 cggacgctgt tggcctcgcg gcgcgcccgc gccgcctcga agaggtcctt ctgcatccgc
      601 aggatctcat cgtgcaggcg tgccggggcg gcgtcgaagg gcaccggctc cttggcgccg
      661 ccggtgcggc gggcgagcat cgcgtgcccg gccgccacgt cccgcggccc gagctccagc
      721 cggaagggca cgccccgcgc ctcccattcg tagtacttgg cgcccggctt catcccctcc
      781 cgcgcgtcca ggtgggtgcg gatccccgcg ccccggagcg cctggcgcca gcggtccgcg
      841 gcctcgagca cgctcgcccg ctccaggtcg gtcttgtaga tcggcacgat caccgcctgg
      901 tactgcgcca ggagcggcgg gcagacgagg ccccggtcgt cgccgtgggt catgatgagg
      961 ccgccgatca ggcgggtcga gacgccccac gacgtgctcc agacgtggtc gatcccgccg
     1021 ctcgcggtct ggaaggtcac gtcgaacgcg cgggcgaagt tctgcccgag gttgtggctc
     1081 gttcccgcct gcagcgcctt gttgtcctgc atcagcgctt cgcaggcgta ggtccggagg
     1141 gcgccggcaa agcgctcgct gtcggacttg aggccggtga gcaccggcat cgccatccac
     1201 ccttccatga acgtccggta gacgccgagc atctgccggg tctcggcctc cgcctcggcc
     1261 tcgctcgcgt gcgcggtgtg gccctcctgc cagaggaatt ccgtgctccg gaggaagagc
     1321 cgggtgcgca tctcccagcg gaccacgttg gcccactggt tgatcaggat cggcaggtcc
     1381 cggtagctct ggatccactt ggcgtacatg gcatagatga tcgtctcgga ggtgggccgc
     1441 accaccagcg gctcctcgag ctccttgccg ccgccgatcg tgaccaccgc ggtctccggc
     1501 gcgaacccct cgacgtgctc cgcctccttc gacaggaagc tctgcggaat gaagagcggg
     1561 aagtacgcgt tctggtgccc ggtatccttg aacatctggt cgagcgcccg ctgcatctgc
     1621 tcccagacgg cgtagccgtt gggacggatg accatgcagc cgcgcaccgg gctgtagtcg
     1681 gccagctcgg cgcgggcgat gagctcgttg taccaggcgc tgaagtcctg cgcgcgggtg
     1741 gtgagggact tgtcgtcggc cattagcggg tcgcgggtcg tgggacgtga gacgggagac
     1801 gggagacgta agacgtgaga cgtaagacgt gagacgtcgg acgtaagacg tgagacgtgt
     1861 gagatgcgtc ggacacggct accggcccgg ccggatggtc ttgagcactt cctgcacgat
     1921 ctctccccgc gccacgggca tctgcccctt cgcgcgcagg tccttgagga tcacctggtg
     1981 cttctcacgg tcatccggcc cgatgacgac cgcgaagcgc gcgttccggg cgtcggccag
     2041 tttgagctgc ttccccaccg cctggacgcc cagggcgtac tcgacccgga tgcccgcgtc
     2101 gcggagctcg tgcgccaggc ggagcaccgc cggcacgtcg ccctcggtga tggcggccag
     2161 gaagacgtcg atcgacgccg cgtgcgccgg gaccagcgcc ttgtccttga ggagctcgcc
     2221 caggacgacg tcgcccatcc cgaagcccac cgcgccgagc tccacgccgc cgatcgcgcc
     2281 ggtgagcgcg tcgtaccggc cgccgccgca gatggcgcgc agcgtgcgcc cggtgtcgaa
     2341 gagctcgaac accgtcccgg tgtagtacgc cagcccgcgg acgatggtcg ggtcgaactc
     2401 gacccagtcg gcgaggccca tgtcgtcgag cgcgtcgagc accgcgccga accgcgacag
     2461 ggtgcccccg cgggccacga gctcctggcg caactcggtg cgggccgacg ggtcgcccag
     2521 ccggcgcacg atctgcgcag ccgacgatgg cgccacgccc tcgagcatcg cccgggcctg
     2581 gtcgtggctc ttctcaaggc ggtcgatggc ctggagcgcg acgccgagcg ccgcatcggg
     2641 cacgccggcc tgccggagcc agtcggtgat caggatccgg tccgagaccc gcacccgcac
     2701 gtcccgggcc gagagcccga aggcccgcat cacgtcgatc gccagcgcga tgatctcggc
     2761 gtcggcgagc ggcgtcgact cgccgatcag gtcgcaattg agctggaagt gctcccgcag
     2821 ccgtccccgc tgctgccgct cgtagcggaa gagctggggg atcgagaacc agcggatcgg
     2881 cttcttgagc ccattggccc gggccgccac catccgcgcc agcgacggcg tcatctccgg
     2941 ccggagcgcc acccgccggt cgcccttgtc ggtgaactca tagagctggc cgacgatctc
     3001 ctcgccgctc ttctcggtgt agaggtccag cgattccagt ggcgggccgt cgtactcctc
     3061 gaagccgtag cgccccgcca cccggcgcca ggtctcgaag atgtgggccc ggagggcgaa
     3121 atcctcagga tagaagtccc ggaatcccgg cagggccgtg gtgggcatac gcaaaggtaa
     3181 tccgggtagg cacttagctc taccccggca acgggcgcca ccggggcctc ccccaccccg
     3241 gtcaaccgtc ggcgtccatc ccgagttcgt gcatcacgtc gccgacggtg tggaagcttc
     3301 cggtgaccag caccgtcccc gcacccgcct ccgcctcgcg cagcgcccgg cggaagtcgg
     3361 cctccaggtg ccaggccgcc cgcgcggggg gacggaccgg atcctccagc caccgttcca
     3421 gccattcgag ggtccagcgc cgctggtccg ccgtcggggc gatggtgagc cacccgcggt
     3481 ccacggcgcg gtcgagctcc accagcatct cggcccattc cttgtccccg aggatcgaca
     3541 ccaccgcgtg gatcgggcgc ggcaggcgtt cctcctcgag ggcgcgcacc agcgcccgca
     3601 tcccatcggg attgtgcgcg acgtcgaaca gccatttccc ccgccggtcg aaccgtcccg
     3661 ggagccgcgc cgccgcgaag cccgccgcca ccgccccctc gtccgggcgg agcgctgcgg
     3721 gcagcgcctc gagcacgcgc cgcgccaccg ccgcgttccg gcgctggtgc ccgccggcaa
     3781 ggcccagcgc cccggtccag cgttccaccg gcggcacggc aacgggcacg ccgcctctgg
     3841 ccagcgcctc ccgccggagg acgtcgacca gggcgggatc ggtctcgccg atgacgaagg
     3901 gcaccccggg cttcgcgatc cccgccttct cccgggcgat ctcctcgagg ctctccccca
     3961 ggtacttcat gtggtcgcgg gcgatcttgg tgacgccggc aacgaggggc gtgatgacgt
     4021 tggtgctgtc gagccgcccg ccgaggccga cctcgaccac cgcgatgtcc gccccgcggg
     4081 ccgcgaagtc ggcgaaggcg atggcggtgc tggcctcgaa gaaggtggcg ccggtctcct
     4141 cgatgggcga ccggagctcc ccggtccacg ccgccaccgc ctcctcgccg atggcgacgc
     4201 catccacccg gatccgctcg cggaacgaga ccaggtgcgg cgaggtgtac tgccccacgc
     4261 ggagcccgag cgccgcgagc gccgccgcga cgagcgtggt cacgctcccc ttgccgttgg
     4321 tgccgccgat gtggatggca gggaactggt cctgcggacg ccccagccgc tcgaggagga
     4381 gtcccgtggt ctccaggccg aacttgatcg tggtggtgcg gggaaagagg aactcgaggg
     4441 cctggggata cgagagcggc atccggggtc cctgggccga gaggtggcga gtgggaggtg
     4501 gagggtggcg ggtgggctta agtttatcgg tgatgccgcg ccaccgcacc ctccccgatc
     4561 cgttcgtcgc ccgcctccgg cagatgcccg ggtaccggga gctgatgacc gattacaccc
     4621 ggtgctccgg ctgcgaaaag tgcgaacgga gcatcgtcta cctgacgccg cgggaacagc
     4681 agcacgcgcg gcacacgggt ctccgcgtct acggccaggg tgccgccgcc cggctcaatc
     4741 gtgccggatg caagtgcccg ttctacgccg ccgagacgtt ccagtgcacg gcgtatctcg
     4801 aacgcccgct tatctgcaac ctctttccca tggacctcct ggagcgggag gaggacggcc
     4861 gccactggtg ggtcctcttc ggcgcctgcg acgaggtccg ggtcggccgc ctgcggggca
     4921 aggtcgaggc cttccgccag ctcgcccgcc gcatcgacgc ggagatgccc gtcgaactca
     4981 agcgctccct cgtcgccgac gccgccggcg ccatccccga gccggcgttc tacgaccacc
     5041 cggtccactg gctggtgccg atgagcgggc tgggtgcggt gagcggtgag cagtgagcgg
     5101 tgaggggtga gcggtgagag gtaagaagtg agcggggggc tccgtacgcc atcggagccc
     5161 cccgcttacc gcttactcct caccgctcac cggtctcacg cgatccgatc cggcacggcg
     5221 tgcccctgca tgtggcggag caggcgcgcc gtggtg
//
LOCUS       DASUAP010000096         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_452652, whole genome shotgun sequence.
ACCESSION   DASUAP010000096 DASUAP010000000
VERSION     DASUAP010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452652"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFU45_02395"
     CDS             <1..474
                     /locus_tag="VFU45_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEU5357943.1"
                     /translation="VIGYGLSGDAYHITSPPEDGAGAAHAIRDCLKDGRLDPARVGYI
                     NAHGTSTPLGDAAETRAVKSVFGEHARKLVFASTKSMTGHLLGGAGALEFVVSLLVAT
                     RGVMPPTINQVTPDPDCDLDSVPNIKRERAVDVALSNSFGFGGHNVTLAVRRFEA"
     gene            550..1479
                     /locus_tag="VFU45_02400"
     CDS             550..1479
                     /locus_tag="VFU45_02400"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEU5357944.1"
                     /translation="MGVIEERDLGFGSVVADRPGHRLLNRDGSFNVRRAGLGLAALSP
                     YHALIKMSWPSFLGLLTAAYLGVNLLFAVLFLACGPGALSAAAADPGMQRFGRAFFFS
                     VETFATIGYGNIVPVGTAANALVVVESLVGLLGVALATGIIFARFSRPRAQVRFSDHA
                     LIAPYRGRTAFEFRIVNERESQMIDVEAKVLLARLVDHGGRKARKFEELELERRRVAF
                     FPLAWTVVHPIDEGSPFRGLTHQDLVESDAEVLILLTGTDETFAQSVHARSSYRAEEI
                     LWGSRFAGMFDPPGPDGVLSVDVSRLDDVEPGA"
     gene            1554..>2338
                     /locus_tag="VFU45_02405"
     CDS             1554..>2338
                     /locus_tag="VFU45_02405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5357945.1"
                     /translation="MSAQLDPAQFRDPALRPIAEKVVAGRRLDPADGLALYVSDDLLA
                     IGALAAWANDRKNGERVFFSANQHINPTNVCILRNTCTFCSFARMPKEDGHYTRSLEE
                     VYHEAEQARGMPTREFHIVGGLHPKLRLSYYTDMLRGLKARHPDVHIKALTAVEIAHL
                     ARIERTTEEDVLLQLKDAGLTSMPGGGAEVFSTAVRATIAERKLTGEEWIRVHRTAHR
                     LGIPTNCTMLYGHVETARDRVEHLTMLRELQDETGGFLTYIPLA"
BASE COUNT          341 a          837 c          802 g          358 t
ORIGIN      
        1 gtgatcggct acggcctctc gggcgatgcg taccacatca cctcgccccc cgaggacggc
       61 gctggcgcgg cccacgccat ccgtgactgc ctcaaggatg gccggctcga ccccgcgcgg
      121 gtcggctaca tcaacgccca cggcacctcg acgccgctgg gcgacgccgc ggagacccgg
      181 gcggtcaagt cggtcttcgg cgagcatgcc cggaagctgg tgttcgcgtc gaccaagtcg
      241 atgaccgggc acctgctggg cggggcgggg gcgctcgaat tcgtcgtgtc gctgctggtg
      301 gccacccggg gcgtgatgcc gcccacgatc aaccaggtga cgcccgatcc cgattgcgac
      361 ctcgactcgg tccccaacat caagcgggaa cgcgcggtgg acgtggcgct ctccaactcc
      421 ttcggcttcg gcgggcacaa tgtcacgctg gcggtgcgcc ggttcgaggc gtgatctggt
      481 gagcggggca cgtccggaga cccttcggcc ctgcggggcc ggggggtcgt tcgcttggga
      541 ggacagcgcg tgggcgtgat cgaagagcgg gacctcggct tcggctcggt ggtcgccgac
      601 cggcccgggc accggctcct caaccgcgat gggagcttca acgtccggcg cgccgggctc
      661 ggcctcgccg cgctgagccc gtaccacgcg ctcatcaaga tgtcgtggcc gtccttcctc
      721 gggctcctca ccgccgcgta tctcggcgtc aacctgctct ttgcggtcct cttcctggcg
      781 tgcggacccg gggcgctgag cgcggccgcc gccgacccgg ggatgcagcg gttcggccgg
      841 gcgttcttct tcagcgtgga gacgttcgcc accatcggct acggcaacat cgtgccggtc
      901 ggcaccgccg ccaacgcgct ggtcgtcgtg gagtcgctgg tcgggctcct gggcgtggcg
      961 ctcgccaccg ggatcatctt cgcgcgcttc tcccggccgc gggcccaggt ccggttctcc
     1021 gaccacgccc tcatcgcccc gtaccggggg aggacggcgt tcgaattccg gatcgtcaac
     1081 gagcgcgaga gccagatgat cgacgtggag gccaaggtcc tgctggcccg gctcgtcgac
     1141 cacggcggcc gcaaggcacg gaagttcgag gaactcgagc tcgagcggcg ccgggtggcc
     1201 ttcttcccgc tcgcgtggac ggtggtccac cccatcgacg aggggagccc gttccggggg
     1261 ctcacccacc aggacctcgt ggagtccgac gcggaggtcc tcatccttct cacgggcacc
     1321 gacgagacct ttgcccagag cgtgcacgcc cgatcgtcct accgcgccga ggagatcctg
     1381 tggggcagcc ggttcgccgg gatgttcgat ccgcccggcc cggacggggt gctgagcgtc
     1441 gacgtctcac gccttgacga cgtggaaccg ggcgcgtgat gaccggctgg cacgctcctg
     1501 ccgcgggtgg ctagattcgg gtgtggcaag atcctgcatc tcaaggggat aggatgtccg
     1561 ctcagctcga ccctgcgcag ttccgggacc ccgcgctgcg gcccatcgcc gagaaggtcg
     1621 tcgccgggcg ccggctcgac ccggccgatg gcctcgcgct ctacgtgtcg gacgacctcc
     1681 tcgcgatcgg cgcgctggcg gcctgggcca acgaccggaa gaacggcgag cgggtcttct
     1741 tctccgccaa ccagcacatc aacccgacca acgtctgcat cctccggaac acctgcacgt
     1801 tctgttcgtt cgcccggatg ccgaaggagg acgggcacta cacccgctcc ctcgaggaag
     1861 tgtatcacga ggcggagcag gcgcgcggga tgccgacgcg ggagttccac atcgtgggcg
     1921 ggctccaccc caagctgcgg ctcagctatt acaccgacat gctgcgggga ctcaaggccc
     1981 ggcaccccga cgtccacatc aaggcgctga cggcggtcga gatcgcgcac ctggcccgga
     2041 tcgagaggac caccgaggag gacgtgctcc tccagctcaa ggatgccggc ctcacctcga
     2101 tgccgggtgg cggggcggaa gtgttcagca ccgccgtccg ggccacgatc gccgagcgga
     2161 agctcaccgg cgaggaatgg atccgcgtcc accggaccgc gcaccggctc ggcatcccca
     2221 ccaactgcac gatgctctac ggccacgtcg agaccgcccg cgaccgggtg gaacacctca
     2281 cgatgctgcg cgagctccag gacgagaccg gcgggttcct gacctacatc ccgctcgc
//
LOCUS       DASUAP010000097         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1283592, whole genome shotgun sequence.
ACCESSION   DASUAP010000097 DASUAP010000000
VERSION     DASUAP010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283592"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VFU45_02410"
     CDS             <1..971
                     /locus_tag="VFU45_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5357946.1"
                     /translation="DGPRVLANMAVDSWVPHRFAALSERLTTQNGIVLMGVAALAALL
                     YTGGQVSHLIVMYSINVFLTFSLSMFAMLKHWRSAKGRPRRGARMTLFAAGFAMCATI
                     LAITVFEKFSEGGWVTVAVTAVLIGVCLWIRRHYRQSSAKIEELYRQLGDLPTTVKPG
                     ATPLALDPKAPTAVMLVASYGGIGIHTLLNIFRAFPGHFKNVVFISVGLVDSGEFKGE
                     HAVDELKARTAEMLAQYVTLAQGLGVAATSRMAVGTEVVESAEHLCLDVAREFPRSTF
                     FAGKMIFQRETWYQGILHNETAFAIQKRLQWAGKTMVTMPVRVREA"
     gene            1087..1614
                     /locus_tag="VFU45_02415"
     CDS             1087..1614
                     /locus_tag="VFU45_02415"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5357947.1"
                     /translation="MRLLSSVSLAALLLMPAAVAAQGGPVSDAARMMAKDNAKFLLAA
                     AEAMPADKYAFKPTPAQMSFGQLIAHIAGDDHFTCSAIAGVKPAPRTRIAPTEGKEKL
                     VAALKASFTECDSALTALKDASLGDTVSWYGGNVTRAMAVFGLLTDWADHYSQQAMYL
                     RLNGILPPTARKGGM"
     gene            complement(2084..2248)
                     /locus_tag="VFU45_02420"
     CDS             complement(2084..2248)
                     /locus_tag="VFU45_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5357948.1"
                     /translation="MGERAGVTRRSVNAIETGKMVPSVLLALKLARALRVSMDELFVL
                     EEDVGGGPPA"
BASE COUNT          344 a          837 c          711 g          370 t
ORIGIN      
        1 tcgacgggcc ccgggtgctg gccaacatgg ccgtggattc ctgggtgccc caccgctttg
       61 ccgcgctctc cgagcggctc accacccaga acgggatcgt gctgatgggc gtggccgcgc
      121 tcgcggcgct gctctatacc ggcggccagg tgagccacct gatcgtgatg tacagcatca
      181 acgtgttcct cacgttctcg ctctcgatgt tcgcgatgct caagcactgg cgcagcgcca
      241 aggggcgccc caggcgcggg gcgcggatga cgctcttcgc ggcggggttc gcgatgtgcg
      301 ccacgatcct cgccatcacc gtcttcgaga agttcagcga gggcgggtgg gtcaccgtgg
      361 cggtcaccgc ggtgctgatc ggcgtgtgcc tctggatccg ccggcactac cgccagtcga
      421 gcgccaagat cgaggagctc taccgccagc tcggcgacct ccccaccacc gtgaagcccg
      481 gcgccacgcc cctcgcgctc gaccccaagg cacccacggc ggtgatgctc gtggcctcat
      541 acggcgggat cgggatccac accctcctta acatcttccg cgccttcccc ggccacttca
      601 agaacgtggt gttcatctcc gtggggctcg tcgactccgg cgagttcaag ggcgagcacg
      661 cggtggacga gctcaaggcc cgcacggcag agatgctcgc ccagtacgtg acgctggccc
      721 aggggctcgg cgtcgccgcg acctcgcgga tggccgtcgg caccgaggtg gtggagtcgg
      781 ccgagcacct ctgcctcgac gtggcgcggg aattcccccg gagcaccttc tttgccggca
      841 agatgatctt ccagcgggag acctggtacc aggggatcct gcacaatgag accgcgttcg
      901 ccatccagaa gcggctgcag tgggcgggga agacgatggt gacgatgccg gtgcgggtac
      961 gggaagcgta ggagtcgggg cggcctgcgg ccaccccctc cgccccctga aacccccggc
     1021 ccgccgcgcc gtcataggat gccagcccgc tcgcccggac caccaccctt ccgcccgagg
     1081 tcctgtatgc gcctgctcag ctccgtctcg ctcgccgcac tcctgctgat gcccgccgcc
     1141 gtcgcggccc agggcggccc ggtctccgac gcggcccgga tgatggccaa ggacaacgcc
     1201 aagttcctcc tcgccgccgc cgaggcgatg cccgcggaca agtatgcctt caagcccacg
     1261 ccggcacaga tgagcttcgg gcaactcatc gcccacatcg cgggtgatga ccacttcacc
     1321 tgctccgcca ttgccggggt caagccggcg ccccggaccc gcatcgcccc gacggaaggc
     1381 aaggagaagc tggtggccgc cctcaaggcg tccttcaccg agtgcgactc cgccctcacc
     1441 gccctcaagg acgcctccct cggcgatacc gtttcctggt acggcgggaa cgtcacccgc
     1501 gccatggccg tcttcggcct cctgaccgac tgggccgacc actacagcca gcaggcgatg
     1561 tacctccgcc tgaacggcat cctgccgccc accgcccgca agggcgggat gtaagggcga
     1621 tcgggatgcc ggactcagga tgcgtagaat ggcgcttgaa ttctgagtct tggctccagc
     1681 gtccccgacg ctcctgactg gatatacgac tgcatcgcag ggctcgggac ccgcggaata
     1741 gccgtcgggt cccgagtgct tctgcctgga tcccctgcgc gcggtatctc ctctcgtctc
     1801 gacactacgg caagcctaga aggcgtcgcc tgcttaccgc gacctcactt ctggcgttcc
     1861 tcgccctctc gctgaccgcg cagtcacccc cacgcgtcta ctccggcgcc ctgcacgact
     1921 gggcgatagc gggtcagggc cactatcacc cgcccggtga ccagccgagc cctgccttcc
     1981 cgatgttggg caccgcatgt ttcgcggcgt ggaccaggaa aatggtccgg ggcgctttcg
     2041 gtgcgaactg atatccgtcg catcgccccc tgacgaccga cgttcaggcc ggcggcccgc
     2101 cccctacgtc ctcctccagc acgaacagtt cgtccatcga cacccggagt gcccgggcca
     2161 gcttgagggc caggagtacg gacggcacca tcttgccggt ctcgatggcg ttgaccgacc
     2221 ggcgtgtcac gccggcgcgc tcccccagct cggcttgggt ga
//
LOCUS       DASUAP010000098        14131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_397733, whole genome shotgun sequence.
ACCESSION   DASUAP010000098 DASUAP010000000
VERSION     DASUAP010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 14131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14131
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397733"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VFU45_02425"
     CDS             complement(<1..1323)
                     /locus_tag="VFU45_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU5357949.1"
                     /translation="MRAPPAVILAFGYGAGLATGLLHFGDPASALLLLAVLSAGARSA
                     LPTAALFLAAMAAGRIAAWGAWQAEPGRCASRLRAGEVRIAGVPRDPPGGLAPVRFYV
                     ADRCVGDILVRWPEGATAEVGQRVTVEGRWIPRAEGPAGRQGGALVVRRSEPDTGSAP
                     APLRWRRRLFATTAALYGSRAPLVDALLFDRRGAVDRGVRNAFAQAGLIHLLSISGFH
                     VGLIVAWVLLAAQGAGLGRSRAWVVATGVALAYVGWLGWPAPATRAAGLAVATCVTRL
                     RQRVPAWSSLLAVTALGVLVVDPWAIFDVGAWLSVGSLWGATHFARWGESALGRRPVW
                     RDLAGSVGATIATAPITAAALGTVALAGIGLNFVGIPLAAIAVPGALASVLVAPVSGV
                     LARALASGTGLALALLDRVAGWGARIPFGHITTATGALSAVPWIGVLVA"
     gene            complement(1340..1612)
                     /locus_tag="VFU45_02430"
     CDS             complement(1340..1612)
                     /locus_tag="VFU45_02430"
                     /inference="COORDINATES: protein motif:HMM:NF022179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HEU5357950.1"
                     /translation="MVEEHVIQRYEALLSSVPGFCGCPLCRSDVMVYALNRLPARYVA
                     TTEGAVLSEISLDKDQSRTVIDVVLLEAFRKVGMAPRCGAKPMPKA"
     gene            complement(1621..2940)
                     /gene="miaB"
                     /locus_tag="VFU45_02435"
     CDS             complement(1621..2940)
                     /gene="miaB"
                     /locus_tag="VFU45_02435"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU5357951.1"
                     /translation="MTARLYIETYGCQMNVADSELMFGVLGRAGYERVDAPEGADVLL
                     VNTCAVRDNAEQRVVGRVGELQRHKRPGGVLGVVGCMAQRLGPRLLERAPKVDLVVGP
                     DAYRHLPAIIAQAQAGERVSDTEFRGWEHYEDVPAVREPGPTAFVTVQRGCDFRCTFC
                     IVPYTRGPERSRKLDDVVREVAGLAGAGTTEVTLLGQTVNSYHDGTHDFADLLRAVGA
                     VDGVRRLRFTSPYPTEFTDRVIDAMARVPAVCEHVHLPAQSGSDSCLRRMRRRYDRGA
                     YLDVVRRLRAAIPGITFSTDLIVGFPGETDLEFADTLSLVAEAGFDEAYTFKYSVREG
                     TPAVRIKGHLDDVVASERLQRLIAAVREQSARRNLARVGEVHEVLVERPARRGDLLLG
                     RTRTNHMVLLDLPASALGQYHTVRLTGTTGSTFTGEPARAARPLAVV"
     gene            complement(2978..4150)
                     /locus_tag="VFU45_02440"
     CDS             complement(2978..4150)
                     /locus_tag="VFU45_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357952.1"
                     /translation="MRRLLLTGALLGCASPLLAQASTFGIRGLGFPNAPYSAHARGLG
                     GSSSLFDTESGMNPAVLGTLRTLTAGITTLPEWRTVESPAGTGSVRGMRFPMIAAAGP
                     IPSSRFAIGISAASYTTRDFSQAFVDTVMLRGVPEVVHDTLTGRGGISDLRLALSWRA
                     TPDLDLGGSLHALTGTDRASLVRHFTDTTAYATASQKTELSYGGFGVDLGAVGRLGPT
                     LDFAAIVRSDGNVSIQRDSLPTKYPVDLPYTFGAGLRYRPSSVLTVTGQGIYRTWSGA
                     NSDLLLQGGTGSRNVWDLSAGAEFLRDASRPYDLPIRLGVRYSDLPFPFAAGDKPSEW
                     ALSVGTGKLFAKGLGGFDAALERVWRSEAGGFSERAWLLTIGVTVRPYFQATPGVR"
     gene            complement(4151..5389)
                     /locus_tag="VFU45_02445"
     CDS             complement(4151..5389)
                     /locus_tag="VFU45_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357953.1"
                     /translation="MIPRLERILRVRVTWALTAAVMAAALATACQEQLTAPAQCPQAC
                     PGGRPVLRDTVLDALPGGDSSFSGYIISGSSLGGVLVSDSLGGVDDRAYLRMLPRPDS
                     VSVKDTARAFTLDSVALALTVLAHDTTVHGLYLFIYRLPASTDSGQTFGAVDSLLTPA
                     ALLDSVAISDTLRSQTVRLLYADTTLAKIAIAPADSGRLALGFRIRATAHTGARLGGI
                     GSGSAVPIMTSYVTVAGDTDTTSRHQSIVRAPEYTKFVERSTYAPNPDLLVVGGQSGS
                     RALVRFPFPEYLKDSVILVRATLQLTPNDTMGGLRGDSTAVIATGILADFGAKSPRFS
                     LTSSTQLFSGSIDTVGVEVVNQVRVWQTATPAVPSAFMMNLYPEGASFALPRFRSTRS
                     GGGAPRLRITYQVPFKFEKP"
     gene            complement(5386..5778)
                     /locus_tag="VFU45_02450"
     CDS             complement(5386..5778)
                     /locus_tag="VFU45_02450"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU5357954.1"
                     /translation="MSEDGVLAAARVAQARAHAPYSHYRVGAAIEAEDGRIFSGCNVE
                     NASYGLTICAERGAVMAAVAAGARAFRRVVVVTDSEPPAAPCGACRQFLAEFGPGLEV
                     EAVGPTSTRRWRLSELLPATFDKGYLER"
     gene            complement(5775..6941)
                     /locus_tag="VFU45_02455"
     CDS             complement(5775..6941)
                     /locus_tag="VFU45_02455"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU5357955.1"
                     /translation="MSRRAAIIVLDGLGIGPAHDTDRYGDAGSDTLGNTLRVVGGAAL
                     PNLAAFGIGNIAPLAGVPAAGHPQAAWGKAQPVSAGKDSTTGHWELCGVILERPFPTY
                     PSGFPREVLEEFSRRTGRPVIGNVAGSGTAMLDRYGEEHLRTGAWIVYTSADSVFQVA
                     AHEERVPLAELYEACATARQILHGPDAVSRVIARPFIGGPGAWKRTAHRKDLSLLPPA
                     PTLLDHLAERGIPRVGVGKVDDLFAGRNITSVHTPTNADAYRRIDEALATMDTGFLFV
                     NVIEFDQTWGHRNDVKGFVGGLHELDAALPALLGRVREEDLIIFTADHGNDPTTPSTD
                     HAREVVPVLAYGPRVRPVPLGERRTFADLGQTVAEFLGARPLAAGTSFLREVWR"
     gene            complement(6938..7183)
                     /locus_tag="VFU45_02460"
     CDS             complement(6938..7183)
                     /locus_tag="VFU45_02460"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEU5357956.1"
                     /translation="MDQHFASLVVGLATQAGQALDGTLAGLPAGASPREAARALIDTL
                     AMLESKTGGRLDDQERQLLTQALTALRFRYVQTEPKQ"
     gene            complement(7183..8262)
                     /gene="alr"
                     /locus_tag="VFU45_02465"
     CDS             complement(7183..8262)
                     /gene="alr"
                     /locus_tag="VFU45_02465"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU5357957.1"
                     /translation="MTTSIASTPERVWVDVDRSALIANARTLAASARVPLLPMIKADA
                     YGLGAIEVARALEAIGPWGFGVATLDEAAALRAARVVRPIIVFTPLAPDQLERYRALE
                     LRPLICDLPALDAWLAADAGPFHLGIDTGMGRVGFHWNDAALRSAVAERLRGAAGWEG
                     IATHFHSADADVEATALQWRRFEEVIGSLPRRPPLVHAANSAAALRGAGYAGGLVRPG
                     IFLYGGSVGPWAPAPRPVATLRSRVVAVREVPAGESVSYRAAWRATEPTTIATLAIGY
                     GDGLLRSGTGRARVELNGAVVPVVGRITMDFTMVAVARGAARIGDVATVFGGAISLDA
                     HADALGTISYEVLTAMSPRVIRRYA"
     gene            8299..9099
                     /gene="mazG"
                     /locus_tag="VFU45_02470"
     CDS             8299..9099
                     /gene="mazG"
                     /locus_tag="VFU45_02470"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU5357958.1"
                     /translation="MQENSALGRALDLVRDLRARCPWDRVQTRSTLRPYLVEEALELD
                     EALASGDGDRIKEEVGDLLLHLAWQFVLAEESREFTPDDAADRLIAKMRRRHPHLFDL
                     GPRESWETLKRRETRRGVLDGLPATLPSLQMSYRLQQKAASVGFDWPDTAGPAAKVRE
                     ELAEVEAELELAGANAVGEAGGADSGVERDAGDPLVEEIGDLLFAVVNLARKTRVQPT
                     VALDRANRKFRRRFETVESLSASRGIDLATAGLDVLDGMWEEAKRGEK"
     gene            complement(9239..10534)
                     /gene="asnS"
                     /locus_tag="VFU45_02475"
     CDS             complement(9239..10534)
                     /gene="asnS"
                     /locus_tag="VFU45_02475"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU5357959.1"
                     /translation="MAAVTISELGGHVGESVTVRGWAMTTRSSGKIAFLVLRDGTGYL
                     QAVFSRKEVSDAVWEAFQRLTQETSLAVTGTVRAEPRSPGGFELTATDLVILGPSVDF
                     PISPKEHGTSFLFEHRHLWLRSRRQVAIARVRNEVVQAIRDFFYERGFVLVDTPILTG
                     SIGEQAGELFATDYFDLGKAYLAQTGQLYGEAAAAALGKIYCFGPTFRAEKSKTRRHL
                     TEFWMVEPEVAWNDSAANMRLQEEFVSYIVARALDRAREPLKELERDTAPLERVTAPF
                     HRISYTDAVAKLNALGSDITWGSDFGGDDETLLTKQFDRPVFVFNYPKAIKAFYMKEN
                     PEDPRTVLCDDCLAPEGYGEIIGGSQREDNYDRLLARITAQGLDPAAYGWYLDLRKYG
                     TFVHSGFGLGLERTVAWICGNPHIRESIAFPRQIHRLYP"
     gene            10586..11635
                     /locus_tag="VFU45_02480"
     CDS             10586..11635
                     /locus_tag="VFU45_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5357960.1"
                     /translation="MGAGLVNAACAYVRPIPDAPLAADTAHPAPFAQQWLVRVNQGFL
                     GPMLLRGDTLIGTGYDRRLVAVDLVRGKKAWENRLNGGASAGVAGLGDTIYAASDRPD
                     GSVEAFHRSNGYDVWRRRHTGRIALPLLLADTFIVAAPRTGNVMALDRASGRGRWVHR
                     MAAATEAPVAGRPGEIVVATDDSLYRLHLSDGKLVAAVRAPGAASAPWVQVDSTLLLP
                     TGTGDLAAIRVADLGVEWRVHFDGPILVAPTVVNDTAWIVTQAGGVYRVPILHPAAER
                     YATLPDPASAPPVPWRGWLLVGMADGTLRALGPDGHEAWRVAVGRPVVQPPIVLPDGL
                     LIVGGRGDLHRMVAR"
     gene            11632..12114
                     /locus_tag="VFU45_02485"
     CDS             11632..12114
                     /locus_tag="VFU45_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357961.1"
                     /translation="MSGRSIIAGLATGIALAATVANALAGQAPVRPSPTIIRYGKWVA
                     LGSSLAFGLLSQAEHADADRAFGALESYCFTDQTRCDTGPDGHYLDPVSEGYYQASLT
                     HDRRAGRWLWGGEALFLSAAAGFIWELARPRGPPENIPFAPLVESYQGRLEVGGSVRF
                     "
     gene            complement(12128..>14131)
                     /gene="recG"
                     /locus_tag="VFU45_02490"
     CDS             complement(12128..>14131)
                     /gene="recG"
                     /locus_tag="VFU45_02490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU5357962.1"
                     /translation="VESALDLLWHLPHRYLDASAVTPLAAARVGMECSCVGRVVAKRI
                     VPMRRLRVFRAVLRDGSGLLECAWPGQPFLDRTIREGQTLLVTGPVRFYHGRQMVPRE
                     FLVLGEGDAEGDADGGTDEPIWGGRVLPVYPATEGLSHKVIRGLIDRHLDELVALARD
                     PFPPGWAAGLGLPELSAALRAVHHPASLAEAEAGRRRLAFDELFDLQLMLARARLLAK
                     RSRAGIRFEPKRTLTTRLRETLPFALTAGQREAVKAIFADMAAPERMHRLLMGDVGTG
                     KTVVALFAMLLAVENGHQAALMAPTELLAEQHADTLTALLKPLGLVPELLLGRHSASE
                     KAAIRARLSSGAAKVVVGTHALLQERVAFARLGLVVIDEQHRFGVEQRAALIGKGAAP
                     DVLLLTATPIPRSLALTVYGDLDASVLRERPPGRGGVKTAVRTGAGREKVFEFVHAQA
                     RAGRQAYVVLPIIAESEKADLRAATTMAEELQARWPDITVGLVHGRLKPEERDGVMRE
                     FRAGRVQVLVSTTVIEVGIDVPNATVMVIEHPARFGLAQLHQLRGRVGRGGNASQCIL
                     LTDGRVPARLKAFAATDDGFEIAELDLKERGMGDLIGQRQSGTFEIRHARLPDDQDLL
                     ARARELARDVVAADPALERPANAALRERAVARYPRAVELFRIG"
BASE COUNT         2088 a         5092 c         5097 g         1854 t
ORIGIN      
        1 cgccaccagc acgccgatcc acgggacggc gctcagcgcg cccgtggcgg tggtgatgtg
       61 cccgaagggg atccgggcgc cccatcccgc cacgcggtcg aggagggcga gcgcgaggcc
      121 ggtcccggag gcgagcgcgc gggcgagcac accggacacc ggcgccacca gcacgctcgc
      181 gagcgccccc ggcacggcga tggccgcaag cgggatcccg acgaagttga gcccgatccc
      241 cgcgagcgcc acggtgccga gggccgcggc ggtgatcggc gcggtggcga tcgtggcgcc
      301 caccgagccg gccaggtcgc gccagaccgg ccgcctcccg agtgcactct ctccccagcg
      361 ggcgaagtgg gtcgcccccc agagcgatcc gaccgacagc cacgcgccga cgtcgaagat
      421 ggcccacgga tcgacgacga gcacgccgag cgccgtgacg gcgaggaggc tcgaccaggc
      481 gggcacgcgc tgccgcagcc gggtgacgca ggtggccacc gcgaggcctg ccgcgcgggt
      541 ggccggggcg ggccacccga gccacccgac gtacgccagt gccacgccgg tggcgacgac
      601 ccacgcgcgt gatcggccga gccccgcccc ctgcgccgcc aggaggaccc acgcgacgat
      661 caggccgacg tggaagccgg agatcgacag caggtggatg agcccggcct gggcgaaggc
      721 attccggacg ccgcggtcca ccgccccgcg ccggtcgaag aggagcgcat cgacaagcgg
      781 cgcccgcgat ccgtacagcg ccgcggtggt ggcaaagagc cggcgccgcc accggagcgg
      841 cgccggcgcc gacccggtat cgggttccga tcgccggacg acgagcgcac ccccttggcg
      901 ccccgcaggc ccctcggccc gcgggatcca ccgaccctcg accgtcaccc gctggccgac
      961 ctctgccgtc gcgccctccg gccaccggac caggatatcg cccacgcacc ggtcggcgac
     1021 gtagaaccgc accggtgcca gcccacccgg cgggtcgcgc ggcacgccgg cgatgcgcac
     1081 ctcgccagcc cggagtcggg aggcacaccg ccccggctcc gcctgccacg ctccccaggc
     1141 ggcgatccgc cccgccgcca tcgccgccag gaagagcgcc gccgtcggaa gcgccgaccg
     1201 cgcgccggcc gacagcaccg cgaggagcag gagcgcgctc gccgggtccc cgaaatgcag
     1261 gaggccggtc gcgagaccgg ccccatagcc gaacgccagg atcacggcgg gcggcgccct
     1321 caccggaccc gcccccgggt caggccttgg gcatcggctt ggcgccgcag cgtggcgcca
     1381 tcccgacctt ccggaacgcc tcgagcagga ccacatcgat gacggtccgg ctctggtcct
     1441 tgtcgagcga gatctcgctc agcaccgcgc cctccgtcgt ggcgacgtag cgggcgggga
     1501 gccgattgag ggcgtagacc atcacgtcgc tccggcagag cggacagccg cagaagccgg
     1561 ggaccgacga gagcagcgcc tcgtagcgct ggatgacgtg ttcttcgacc atgttccgga
     1621 tcacacgacc gccaaggggc gagccgctcg cgcgggttcc ccggtgaagg tggagccggt
     1681 cgtcccggtg aggcgcaccg tgtggtattg ccccagggcc gaggccggca ggtcgagcag
     1741 gaccatatgg ttggtgcggg tgcggccgag caggaggtcg ccgcggcggg ccggacgctc
     1801 gaccagcacc tcgtggacct cgcccacccg ggccaggttc cgccgggcgg actgctcgcg
     1861 cacggcggcg atgaggcgct ggagccgctc gctggcgacg acgtcgtcca ggtgtccctt
     1921 gatccgcacc gcgggcgtgc cttcgcgcac cgaatacttg aaggtatacg cttcgtcgaa
     1981 gcccgcctcg gccacgagcg agagcgtgtc ggcgaactcc aggtcggtct cgccggggaa
     2041 gccgacgatg aggtcggtcg agaaggtgat gccagggatg gcggcgcgca ggcgccggac
     2101 cacgtcgagg taggcgcccc ggtcgtagcg ccgccgcatc cgccgcaggc agctgtcgga
     2161 gccgctctgc gcgggaaggt gcacgtgctc gcagaccgcg ggcacccggg ccatcgcgtc
     2221 gatgacgcgg tcggtgaact ccgtgggata ggggctcgtg aaccgcaggc gccggacgcc
     2281 gtccaccgcg ccgaccgcgc ggaggaggtc ggcgaagtcg tgggtgccgt cgtgatagct
     2341 gttgacggtc tggccaagga gcgtcacctc cgtggtgccg gcgccggcca gcccggccac
     2401 ctcgcgcacc acgtcgtcca acttacggct ccgctccggg ccccgcgtat acggaacgat
     2461 gcagaaggtg caccggaaat cgcagccccg ctggacggtg acgaacgccg tgggcccggg
     2521 ctcgcgcacg gcgggcacgt cctcgtaatg ctcccagccc cggaactcgg tgtcgctgac
     2581 ccgctcgccg gcctgcgcct gcgcgatgat ggccgggaga tggcgatagg cgtctggccc
     2641 cacgacgagg tcgaccttgg gcgcccgctc cagaagccgg gggccgaggc gctgcgccat
     2701 gcaccccacc acaccgagga ccccgccggg acgcttgtgg cgctggagct cacccacccg
     2761 ccccaccacc cgctgctccg cattgtcccg cacggcgcag gtgttgacga gcagcacgtc
     2821 ggcgccctcc ggggcgtcga cgcgctcgta cccggcgcga ccgagcaccc cgaacatcag
     2881 ctcggagtct gccacgttca tctggcagcc gtaggtctcg atgtagaggc gcgcggtcat
     2941 cgggccaaag atagcggcgg gccggccggg tggcggatca acggaccccg ggcgtggcct
     3001 ggaagtaggg ccgcacggtc acgccgatgg tgaggagcca ggctcgctcg ctgaaccccc
     3061 cggcctcgct ccgccagacc cgctcgagcg ccgcgtcgaa gccgcccagg cccttggcga
     3121 agagcttgcc ggtcccgacc gagagcgccc actcgctggg cttgtcgccg gcggcgaatg
     3181 ggaacgggag gtcggagtag cgcacgccga ggcggatcgg gaggtcgtac ggccgggacg
     3241 cgtcgcgcag gaactcggcc ccggcggaga ggtcccacac gttccggctc ccggtgccgc
     3301 cctgcaggag caggtcgctg ttggcgcccg accaggtccg gtagatccct tggccggtga
     3361 cggtcaggac gctcgacggg cggtaccgga gcccggcgcc gaaggtgtac ggcaggtcga
     3421 ccggatactt ggtcgggagc gagtcgcgct ggatgctgac gttgccgtcg gaccgcacga
     3481 tggcagcgaa gtcgagcgtg ggcccgagcc ggcccaccgc gcccaggtcc accccgaacc
     3541 cgccgtagga cagctccgtc ttctgcgacg cggtggcata ggccgtggtg tcggtgaagt
     3601 ggcggaccag ggaggcccga tcggtgccgg tcagcgcgtg gagcgacccg ccgaggtcga
     3661 ggtcgggcgt ggcgcgccat gaaagcgcca gccggaggtc gctgatgccg ccgcgtccgg
     3721 tgagggtgtc gtgcaccacc tccggcaccc cgcgcagcat caccgtatcc acgaaggcct
     3781 gggagaagtc tctcgtggtg taggacgcgg ccgagatgcc gatcgcgaag cgggaacttg
     3841 ggatggggcc ggccgccgcg atcatcggga agcgcatccc gcgcaccgac ccggtgccgg
     3901 cgggggactc gaccgtccgc cactcgggca gcgtcgtgat gccggcggtg agcgtacgca
     3961 acgtgccgag gaccgccggg ttcatcccgg actcggtgtc gaagagcgaa gagctcccgc
     4021 cgaggccgcg ggcgtgggcc gaatacgggg cgttggggaa cccgagaccg cggatgccga
     4081 aggtcgaggc ctgggccaac agtggcgagg cgcaaccgag cagcgcgccg gtcaggagga
     4141 ggcggcgcac tcagggcttc tcgaatttga aggggacctg gtaggtgatg cggagccggg
     4201 gggcgccccc gccggagcgg gtggacctga accgcggcag ggcgaaactc gcgccctcgg
     4261 ggtagaggtt catcatgaac gccgagggca ccgccggcgt cgccgtctgc cagacccgga
     4321 cctggttgac cacctcgacg cccaccgtgt cgatcgagcc ggaaaagagc tgggtcgagc
     4381 tcgtcagcga gaaccggggc gacttggcac cgaagtcggc caggatgccg gtcgcgatga
     4441 cggcggtcga gtcgccccgg agcccgccca tcgtgtcatt gggcgtcagc tgcagcgtgg
     4501 cgcggaccag gatgacggaa tccttgaggt actccgggaa cgggaaccgc accagcgccc
     4561 ggctcccgct ctgcccgccc accacgagga ggtcggggtt gggcgcgtac gtggagcgct
     4621 cgacgaactt ggtgtactcg ggcgcgcgga cgatcgactg gtgcctgctg gtggtgtcgg
     4681 tgtcgccggc cacggtcacg tacgaggtca tgatcggcac cgccgagccg ctgccgatgc
     4741 cgccgaggcg ggcgccggtg tgggcggtgg cgcggatccg gaatccgagt gccaggcgcc
     4801 cgctgtccgc cggcgcaatg gcgatcttcg cgagggtggt gtcggcgtag agcagccgca
     4861 ccgtctgcga ccggagggtg tcgctgatcg ccacgctgtc gaggagtgcc gcgggcgtga
     4921 ggagggagtc gacggcgccg aacgtctgcc ccgagtcggt cgaggccggc aaccggtaga
     4981 tgaagaggta gagcccatgg accgtggtgt cgtgggccag caccgtcagg gcgagcgcca
     5041 ccgaatcgag cgtgaacgcg cgcgcggtgt ccttgacgct caccgagtca gggcgcggga
     5101 gcatccggag gtacgcccgg tcgtccaccc cgcccaggct gtcggacacc aggacgccgc
     5161 cgaggctcga gcccgagatg atgtaaccgc tgaagctgct gtccccgccg gggagcgcat
     5221 ccagcaccgt gtcgcggagc accgggcgcc cgccagggca ggcctgcgga cactgggccg
     5281 gcgccgtgag ctgctcctgg cacgccgtgg ccagcgccgc agccatcacc gctgccgtga
     5341 gcgcccaggt cacccggacc ctcaggatcc gctccagtcg tgggatcatc gttcgaggta
     5401 gcctttgtca aatgtcgcgg gcagcagctc cgacaaccgc caccgccgcg tgctcgtggg
     5461 acccacggcc tcgacctcga ggccgggccc gaattcggcc aggaactgcc ggcaggcccc
     5521 gcagggcgcc gctggcggct cggaatcggt cactaccacc acccgacgga aggcccgcgc
     5581 ccccgccgcc accgcagcca tcaccgcacc acgttcggcg cagatggtaa ggccgtacga
     5641 cgcgttctcg acgttgcacc cgctgaagat gcgtccgtcc tcggcttcga tcgccgcacc
     5701 gacgcgatag tgggagtagg gcgcatgcgc tcgggcctgc gccacccggg cggcggcgag
     5761 cacgccgtct tcactcatcg ccacacctcg cgcaggaagg aggtgcctgc cgccaggggc
     5821 cgggcgccga ggaactccgc gaccgtctgg ccaaggtcag cgaaggtgcg gcgctccccc
     5881 aggggcaccg gccgcacccg cgggccatac gccaggaccg ggaccacctc gcgtgcatgg
     5941 tcggtcgagg gcgtggtggg gtcgttcccg tggtcggcgg taaagataat gaggtcttcc
     6001 tcccgcaccc ggcccaggag cgctggaagg gccgcgtcaa gctcatggag cccgcccacg
     6061 aaccccttca cgtcgttccg gtggccccag gtctggtcga actcgatcac gttgacgaac
     6121 aggaacccgg tgtccatggt ggccagcgcc tcgtcgatcc ggcggtaggc gtcggcgttg
     6181 gtgggcgtgt ggacgctcgt gatgttccgc cccgcgaaga ggtcatcgac cttccccacc
     6241 ccgacccgcg ggatgccgcg ctccgccagg tggtcgagga gcgtcggcgc gggtgggagg
     6301 aggctcaggt ccttccggtg cgccgtgcgc ttccacgcgc cgggcccgcc gatgaatggc
     6361 cgcgcgatga cccgcgagac ggcatcaggg ccgtgcagga tctggcgcgc cgtggcgcag
     6421 gcctcgtaga gctcggcgag cggcacccgc tcctcgtgcg ccgcgacctg gaagacgctg
     6481 tcggccgagg tgtacacgat ccaggccccg gtgcggaggt gctcctcgcc gtagcggtcg
     6541 agcatcgcag tgcccgatcc cgccacattg ccgatcacgg gcctgccggt gcgacgggag
     6601 aattcctcca gtacctcccg cgggaagccc gagggatagg tggggaacgg ccgctccagg
     6661 atgacgccgc acagctccca gtgccccgtc gtgctgtcct tcccggcgct cacgggctgc
     6721 gccttccccc aggcggcctg gggatgtccg gccgccggca cacccgccag cggggcgatg
     6781 ttcccgatgc cgaaggccgc gaggttcggg agcgcggcgc ctccgacgac ccgcagggtg
     6841 ttgccgaggg tgtcgctgcc cgcatcgccg tagcgatcgg tgtcgtgggc ggggccgatc
     6901 cccaggccgt ccaggacgat gatcgcggcg cgccggctca ctgcttcggc tcggtctgca
     6961 cgtaccggaa gcggagcgcc gtgagcgcct gggtcaggag ctggcgctcc tggtcgtcca
     7021 gccggccgcc ggtcttcgac tcgagcatcg ccagcgtgtc gatgagcgcg cgggccgcct
     7081 cgcgcggcga ggcgcctgcc ggcagcccgg ccagggtgcc gtcgagggcc tgccccgcct
     7141 gggtggcaag cccgaccacg agcgaggcga agtgctggtc catcaggcgt acctgcgaat
     7201 gacccggggg ctcatcgccg tcagcacctc gtaggagatg gtgcccagtg cgtccgcgtg
     7261 ggcgtcgagc gagatggcac cgccgaacac ggtggccacg tcgccgatcc gggcagcgcc
     7321 cctcgcgacc gccaccatcg tgaagtccat cgtgatcctt cccaccaccg gcacgaccgc
     7381 gccgttgagc tccacccgcg ctcggccggt gccgctgcgc agcaggccgt cgccgtagcc
     7441 gatggcgagg gtcgcgatcg tcgtcggttc ggtggcccgc cacgcggcac gatagctcac
     7501 cgactccccg gcggggactt cccggaccgc caccacccgg ctccgcagcg tggccaccgg
     7561 gcggggcgcc ggcgcccacg gcccgacgct cccaccataa aggaagatcc cggggcggac
     7621 gagccccccc gcataccccg cgccccgcag cgccgcggca ctgttcgccg cgtggaccag
     7681 gggggggcgg cgcggaagcg aaccgatgac ctcctcgaac cgtcgccact gcagcgcggt
     7741 ggcctcgacg tcggcatcgg cggagtggaa atgggtggcg atcccttccc atcccgccgc
     7801 tccgcgcagc cgctccgcga ccgcagagcg cagcgccgcg tcgttccagt ggaaccccac
     7861 ccgccccatc ccggtgtcga tgccgaggtg gaatggcccc gcgtcggcag cgagccacgc
     7921 atcgagcgcg gggaggtcgc agatgagggg gcggagttcg agggcgcggt agcgctccag
     7981 ctggtcgggc gcgagcggcg tgaagacgat gatcgggcgg accacacgag ccgcccggag
     8041 cgcagcagcc tcatcgagtg tcgcgacgcc gaagccccag ggaccgatcg cttccaaggc
     8101 gcgggcgact tcgatggcgc cgagcccgta ggcgtcggcc ttgatcatcg gcaggagcgg
     8161 cacccgggcc gaagcggcca gggtccgcgc gttggcgatc agcgctgaac ggtcgacatc
     8221 gacccagacg cgctcgggcg tggaggcgat tgacgtagtc acgatccggg cggtatgata
     8281 accgacgccg gaggctaaat gcaagagaat tcagcgcttg gacgcgccct cgacctggtg
     8341 cgcgacctgc gggcacgctg cccgtgggac cgggtccaga cccgcagcac gctccgccca
     8401 tatctggtcg aggaggcgct ggagctcgac gaagccctgg cctccggtga cggcgaccgg
     8461 atcaaggagg aggtgggcga cctcctgctc cacctggcct ggcagttcgt gctggcagaa
     8521 gaatcccgcg agttcacccc cgacgacgcc gccgaccggc tcatcgccaa gatgcgccgc
     8581 cgccaccccc atctcttcga cctcggcccc cgcgaatcgt gggagacgct caagcgccgc
     8641 gagacccgcc gcggcgtgct cgacggcctc cccgccacct tgccctcgct ccagatgtca
     8701 tatcggctgc agcagaaggc cgcgtcggtc ggcttcgact ggccggacac cgccggcccc
     8761 gccgccaagg tgcgggaaga gctggcggag gtggaggcgg aactggaact ggcgggcgcg
     8821 aacgccgtgg gggaggccgg gggcgcggac agcggcgtcg agcgcgatgc cggcgatccg
     8881 ctggtggagg agatcggtga cctgctcttc gcggtcgtga acctggcgcg caagacccgg
     8941 gtccagccca ccgtggcgct cgaccgggcc aaccgcaagt tccggcggcg gttcgagacg
     9001 gtcgagtccc tgtcggcgag ccgcggcatc gacctcgcca ccgcgggcct cgacgtgctg
     9061 gacgggatgt gggaggaggc gaagagaggt gagaagtgag aagtgagaag tgagaagtga
     9121 gaagtgagaa gttccgagga ttgcctgccg gcccaaaatg cgcaagaggg cgcgcgatag
     9181 atcggcgccc cctcttccct ctcacctgtt ccctcttccc tcttccctcg cacctctcct
     9241 acggatacag ccgatggatc tgccgcggga acgcgatgct ctcgcggatg tgggggttgc
     9301 cgcagatcca cgccaccgtg cgttcgaggc ccagcccgaa gccggaatgc acgaacgtgc
     9361 cgtatttccg gaggtcgagg taccagccgt aggcggccgg atcgagcccc tgggccgtga
     9421 tccgcgccag gagccggtcg taattgtcct cgcgctgtga accgccgatg atctcgccgt
     9481 atccctcggg ggccaggcag tcgtcgcaga gcaccgtccg cgggtcctcc gggttctcct
     9541 tcatatagaa ggccttgatg gccttcgggt agttgaagac gaacaccgga cggtcgaact
     9601 gcttcgtgag gagcgtctcg tcgtcgccgc cgaagtcgct gccccaggtg atgtcagagc
     9661 cgagcgcatt aagcttggcg acggcatcgg tgtaggagat gcggtggaac ggcgccgtga
     9721 cccgctcgag cggggccgtg tcccgttcga gctccttgag cggttcccgc gcccggtcga
     9781 gcgcgcgggc cacgatgtag gagacgaact cctcctgcag gcgcatgttg gcggcggaat
     9841 cgttccacgc cacctccggc tccaccatcc agaactcggt caggtgccgc cgcgtcttcg
     9901 acttctcggc acggaacgtg gggccgaagc agtagatctt gccgagcgcc gccgccgccg
     9961 cctcgccgta gagctgcccc gtctgggcca ggtacgcctt gccgaggtcg aagtagtcgg
    10021 tggcgaagag ctcccccgcc tgctccccga tcgagccggt gaggatcggg gtgtccacca
    10081 ggacgaaccc gcgttcgtag aagaagtcgc ggatggcctg caccacctcg ttccggaccc
    10141 gggcgatggc cacctggcgc cggctccgga gccagagatg tctatgttcg aagaggaaac
    10201 tcgtcccatg ttctttaggc gagatgggaa agtcgacgct cggcccgagg atgacgaggt
    10261 ccgtggccgt gagctcgaac ccgccgggcg acctgggctc cgcccggacc gtccccgtca
    10321 ccgccaggga ggtctcctgc gtcaggcgct ggaaggcctc ccagaccgca tcggacactt
    10381 ccttgcggct gaacaccgcc tgcaggtagc cggtgccgtc gcgcagcacg aggaacgcga
    10441 tcttcccgct cgaccgcgtg gtcatggccc agccgcggac ggtcacggac tcgccgacgt
    10501 ggccgccgag ctcggagatg gtcactgccg ccatcaaagg atccgtggaa tgggacgagg
    10561 actgcgacga gcgtggagtg tagccgtggg cgcggggctg gtcaacgcgg cctgcgccta
    10621 cgtgcggccc atcccggacg caccgctcgc ggccgacacc gcccatccgg cgccgttcgc
    10681 acagcagtgg ctggtccggg tcaatcaggg cttcctcggc ccgatgctcc tccggggcga
    10741 cacgctcatc ggcaccggct atgaccgccg cctcgttgcg gtcgacctcg tccgaggcaa
    10801 gaaggcctgg gagaatcggc tcaacggtgg cgcgtccgcc ggcgtggccg gcctgggcga
    10861 cacgatctac gccgccagcg accgtcccga cggctcggtg gaggcgttcc atcgcagcaa
    10921 cgggtatgac gtctggcgcc gccgtcatac cggccgcatc gccctgccgc tcctgctcgc
    10981 cgacacgttc atcgtcgccg cgccgcggac cggcaacgtc atggcgctcg accgcgccag
    11041 cggccggggc cgctgggtcc accggatggc ggcggccacc gaggccccgg tggcgggccg
    11101 gccgggagag atcgtcgtgg ccaccgacga ctcgctctac cgcctgcacc tctcggacgg
    11161 aaagcttgtg gccgccgtgc gggctccggg agccgcgtcg gcgccgtggg tgcaggtcga
    11221 ttccacgctc ctcctgccca ccggcaccgg cgacctcgcg gcgatccgcg ttgccgacct
    11281 cggggtcgaa tggcgagtcc acttcgacgg cccgatcctc gtggcgccca ccgtcgtgaa
    11341 cgatacggcg tggatcgtca cccaggccgg cggcgtctac cgcgtcccca tcctccaccc
    11401 ggcggcggag cgctacgcca cgcttccgga cccggcgagc gcgccgccgg tgccgtggcg
    11461 cgggtggctc ctggtcggaa tggccgatgg gacgctccgc gccctcggcc ccgatggcca
    11521 cgaggcgtgg agggtggcgg tcgggcggcc ggtcgtccag ccgccgatcg tgcttcccga
    11581 cggtctcctg atcgtcggcg gccgcggcga cctgcaccgg atggtggccc gatgagcggg
    11641 cgctcgatta tcgccggcct ggcgacgggc atcgcgctgg ccgccaccgt cgccaatgcc
    11701 ctcgcaggtc aggcgccggt ccggccatcc ccgacgatca tcaggtacgg gaaatgggtg
    11761 gcgctcggga gctcgctcgc cttcgggctc ctgagccagg ccgagcacgc cgacgccgac
    11821 cgcgcattcg gcgcgctcga gagctactgc ttcaccgacc agacccgctg cgacacgggg
    11881 ccggacgggc actacctcga ccccgtgtcg gagggatact accaggcatc gctgacccac
    11941 gaccggcggg cggggcgctg gctctggggc ggcgaggcgc tcttcctgag cgccgccgcg
    12001 ggattcatct gggagctcgc gcggccgcgc ggccccccgg agaacatccc gttcgcgccg
    12061 ctggtcgagt cctaccaggg ccggctcgag gtgggtggat cggtccggtt ctgacgcggg
    12121 ccggcgatca gccgatccgg aagagctcca ccgccctcgg atagcgggcc accgcccgct
    12181 cccggagcgc tgcgttggcc ggccgctcga gcgccgggtc ggccgccacc acgtcccgcg
    12241 ccagctcacg cgcccgtgcg aggaggtcct ggtcgtcggg gagccgggcg tggcggatct
    12301 cgaaggtccc cgactgccgc tggccgatca ggtcgcccat cccccgctcc ttcaggtcca
    12361 gctccgcgat ctcgaacccg tcgtcggtgg cggcaaaggc cttgagccgc gccggcacgc
    12421 gcccgtcggt gagcaggatg cactggctcg cattcccgcc ccgccccacc cgcccccgca
    12481 gctggtgcag ctgcgcgagc ccgaaccgcg ccggatgttc gatgaccatc accgtggcat
    12541 tggggacgtc gatcccgacc tcgatgaccg tggtggacac gagcacctgc acccgcccgg
    12601 cgcggaactc gcgcatcacg ccgtcgcgct cctcgggctt gagccgcccg tgcaccaggc
    12661 cgacggtgat gtcaggccat cgcgcctgca gctcctcggc catcgtcgtg gccgcccgca
    12721 ggtcggcctt ctccgactcc gcaatgatcg gcagcaccac gtaggcctgc cggccggccc
    12781 gcgcctgggc gtggacgaac tcgaacacct tctcccggcc ggcgccggtc cgcaccgccg
    12841 tcttgacgcc cccccgcccg ggcggccgct cccggagcac cgaggcatcg aggtcgccgt
    12901 acaccgtgag cgccagtgac cgcgggatcg gcgtggcggt gaggagcagg acgtccggcg
    12961 ccgccccctt cccgatcagc gccgcgcgct gctcgacacc gaagcgatgc tgctcgtcga
    13021 tgacgaccaa gccgagccgg gcgaacgcga cccgctcctg cagaagcgcg tgggtaccga
    13081 ccaccacctt ggcggctccc gaggagagcc gcgcgcggat ggcggccttc tcgctcgcgg
    13141 agtggcggcc gagcaggagc tcgggcacga gcccgagcgg cttgagcagc gcggtcaggg
    13201 tgtcggcgtg ctgctcggcc agcagctccg tcggcgccat cagtgcggcc tgatggccgt
    13261 tctcgaccgc cagcagcatc gcgaagaggg ccacgaccgt cttaccggtg ccgacatcgc
    13321 ccatcagcag tcggtgcatc cgctccggcg ccgccatgtc ggcgaagatg gccttgaccg
    13381 cttcgcgctg ccccgccgtg agggcgaacg ggagcgtctc cctgagccgg gtcgtcaagg
    13441 tgcgcttcgg ctcgaaccgg atcccggcgc gggaccgctt ggcgaggagc cgggcgcgcg
    13501 ccagcatcag ctggaggtcg aagagctcgt cgaacgcgag ccgacggcgc cccgcttcgg
    13561 cctcggccag gctcgccggg tgatgcaccg cgcggagtgc cgccgaaagc tccggcagcc
    13621 cgagcccggc ggcccatccg ggggggaacg gatcccgggc gagcgccacg agttcatcga
    13681 ggtgccggtc gatgagcccg cggatgacct tgtggctgag gccctcggtg gccggataca
    13741 ccggcagcac ccgaccgccc cagatcggct cgtcggtgcc gccatcggca tcgccttctg
    13801 catcgccttc accgagcacc aggaattcgc gcggcaccat ctggcggccg tgatagaacc
    13861 ggaccgggcc tgtcaccagc agggtctggc cttcccggat ggtgcggtca aggaagggtt
    13921 ggcccggcca cgcacactcc agcagcccgc tcccgtcgcg cagcacggcg cggaacaccc
    13981 ggagccggcg catcgggacg atccgtttgg ccaccacccg cccgacgcag ctgcactcca
    14041 tcccgacccg cgcggcggcg agcggcgtca cggcggacgc atcgaggtag cggtgcggca
    14101 ggtgccagag caggtccagc gccgactcga c
//
LOCUS       DASUAP010000099         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_983805, whole genome shotgun sequence.
ACCESSION   DASUAP010000099 DASUAP010000000
VERSION     DASUAP010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983805"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..619
                     /gene="arfB"
                     /locus_tag="VFU45_02495"
     CDS             182..619
                     /gene="arfB"
                     /locus_tag="VFU45_02495"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEU5357963.1"
                     /translation="MPFPSDAIPVNARVHVPRAELEFRASRAGGPGGQHVNTSSSRVE
                     LRWDVGRSAALSADERERVIRKLGRRVDSTGTLRIVSDSRRSQLQNREAAVARFQELV
                     RAALATPRARKATRPTKASRERRLAEKRRRTLRKAERRRPSDD"
     gene            complement(645..1673)
                     /locus_tag="VFU45_02500"
     CDS             complement(645..1673)
                     /locus_tag="VFU45_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEU5357964.1"
                     /translation="MTGTVSYVLMIIGVAFVFDFFNGFHDSANSIATVVSTRVLSPGV
                     AVLWAAFFNFAAAFTVGPAVAKTIGGGLVNPAVVDPPLILAALVGAIVWNIITWLLGL
                     PTSSSHALIGGLGGAAVAKAGVGALLLTGWIAPVVAIVVSPFMGMFLGLGLTVGLAWA
                     LHRLPGRPVEVAFRRLQLVSSALFSLSHGANDAQKTMGVIVGLLVATQTAFAQQTGWL
                     HHLHVTNANTIPLWVEMSAYSAIALGTAAGGWRIVKTMGTRITKLRPFGGFCAETGGG
                     ACILVASALGVPVSTTHTITGAIVGVGASQRMSAVRWGVAGRIVWAWILTMPMAAAMA
                     AIAYLVIR"
     gene            complement(1676..2296)
                     /locus_tag="VFU45_02505"
     CDS             complement(1676..2296)
                     /locus_tag="VFU45_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEU5357965.1"
                     /translation="MRLLPRDERFFDLFTQVATLNVEAAVQLVALITAPEDRRAPIVE
                     AIKRLEHQADDLTHEVVNRLDRSFITPLDREDIHMLASRLDDVLDLIDGTARRLQIFR
                     AGDAPTGAIRLAEVIRHAAEQVLEAVKVLEKNDHGVVVDACMKIKKLEEDGDATYHEW
                     LGKLFEGATDALMVMKWKEIYDNLEKTLDHAEDVANVLESISIKHA"
     gene            complement(2389..3924)
                     /locus_tag="VFU45_02510"
     CDS             complement(2389..3924)
                     /locus_tag="VFU45_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU5357966.1"
                     /translation="MLSRVQATAVLGVDAYLVDVEVDITNGLPSFSVVGLPHGAVREG
                     RERVFPALANAGYSVPLKKVTVNLAPADVRKDGSALDLPIALGVLAAGGQVPEDALKG
                     EVFLGELGLEGDLRPVRGALSMALAARAAGCRGIVLPEQNLREAAVVDGLAVRGARTL
                     LDVCRHLTGEAELAVAHVDLAALMAERHQDVVDFNDVKAQVSAKRALEVAAAGGHNIL
                     LIGPPGAGKTMLARRLPTILPPMDLDEALETTKIHSVAGLLAPGQSLSVIRPFRAPHH
                     TISDAGLIGGGSNPRPGEVSLAHGGVLFLDELPEFRKNVLEVLRQPLEDGHVTLSRAA
                     VSLTYPARFILAAAMNPCPCGYHGDPTHSCHCPPEAVERYLARISGPLLDRIDIHLEV
                     PAVNYRDLSSEAAVESSADIRVRVEQARARQRERFRGRPGIHANAQMGARDLRRWCRV
                     GEDVEGLLRSAITRLGLSARAYHRVLKLARTIADLDGAADLAPPHVREAIQYRSLDRR
                     ATA"
BASE COUNT          651 a         1540 c         1455 g          577 t
ORIGIN      
        1 gccacggcca gcagggcgga atcgaccccg accgagtacc cgagcaccac tcgcccgagt
       61 cctgaaaggt gggcgcgaag ccgtgggagg tcggtcattc ttccaaacgt aatcgcgggc
      121 caccgacggg gggagcggtg ccacgcttac ccggcggggc ggcgcgcgtt agcatccagc
      181 gatgcccttc ccgtccgacg ccatccccgt caacgcgcgg gtccacgtgc cccgcgccga
      241 gctcgagttc cgcgcctcgc gcgccggcgg gcccggcgga cagcacgtga acacctcgtc
      301 cagccgggtc gagctccgct gggacgtcgg tcggtcggcc gcgctctccg ccgatgagcg
      361 cgagcgcgtc atccgcaagc tcggccggcg cgtggactcg accgggacgc tcaggatcgt
      421 ctccgactcc cgccgcagcc agctccagaa ccgcgaggcc gccgtggccc gctttcagga
      481 gctggtgcgc gccgcgctcg ccacccccag ggcccggaag gcgacgcgac ccaccaaggc
      541 ctccagggag cgccgcctgg ccgagaagcg gcgccgcacc ctccgcaagg cggaacgccg
      601 gcgtcccagc gacgactgag cggcgccgtc ggacgtccgc ccgatcagcg gatgacgagg
      661 taagcgatgg cggccatcgc cgccgccatg ggcatcgtga ggatccaggc ccacacgatg
      721 cggccggcca ccccccaccg caccgccgac atccgctgcg aggcgccgac gcccacgatg
      781 gcgccggtga tggtgtgggt ggtgctcacc ggcacaccca gcgccgacgc caccaggatg
      841 cacgcgccgc cgccggtctc ggcgcagaag ccgccgaagg gccgcagctt ggtgatccgc
      901 gtccccatcg tcttgacgat gcgccacccg cccgccgccg tgccaagggc gatggcgctg
      961 tacgccgaca tctcgaccca gagcgggatc gtgttcgcgt tggtgacgtg gaggtggtgg
     1021 agccaccccg tctgctgggc gaaggcggtc tgcgtcgcca ccaggagccc gacgatgacg
     1081 cccatcgtct tctgcgcgtc gttggcgccg tggctcagcg agaagagcgc cgaggagacg
     1141 agctggagcc gacggaatgc gacctccacc gggcgcccgg ggagccggtg cagcgcccac
     1201 gcgagaccga cggtgagccc gaggcccagg aacatcccca tgaacggcga caccacgatc
     1261 gccacgaccg gggcgatcca ccccgtgagc agcagcgcgc cgacccccgc cttggccacc
     1321 gccgcccccc cgagcccgcc gatcagcgcg tgcgacgaac tcgtcggcag gccgaggagc
     1381 caggtgatga tgttccacac gatggcgccc accagggcgg caaggatcag cgggggatcc
     1441 accaccgcgg ggttcaccag gccgccgccg atcgtcttgg ccacggcggg accgaccgtg
     1501 aacgccgcgg cgaagttgaa gaacgcggcc cagagcaccg ccacgcccgg cgaaaggacg
     1561 cgggtcgaga ccaccgtggc gatggaattg gcgctgtcgt ggaagccgtt gaagaagtcg
     1621 aagacgaacg cgacgccgat gatcatcagg acgtacgaga cggtgcccgt catcgtcagg
     1681 cgtgcttgat ggagatggac tcgagcacgt tcgccacgtc ttcggcgtgg tcgagcgtct
     1741 tctcgaggtt gtcgtagatc tccttccact tcatgaccat cagcgcgtcg gtggccccct
     1801 cgaagagctt gcccagccac tcgtggtagg tggcgtcgcc gtcctcctcc agcttcttga
     1861 tcttcatgca cgcgtcgacc accacgccgt ggtcgttctt ttcgagcacc ttcaccgcct
     1921 cgagcacctg ctcggccgca tgccggatga cctcggccag ccggatggcc ccggtcggcg
     1981 catcgcccgc ccggaagatc tgcagccggc gcgccgtgcc gtcgatgagg tcgagcacgt
     2041 cgtccaggcg cgaggcgagc atgtggatgt cctcgcggtc gagcggcgtg atgaagctcc
     2101 ggtcgagccg gttgaccacc tcgtgggtca ggtcgtcggc ctggtgctcc aggcgcttga
     2161 tggcctcgac gatcggcgcc cgccggtctt cgggcgccgt gatgagcgcc accagctgca
     2221 ccgcggcttc gacgttgagc gtggcgacct gggtgaacag gtcgaagaac cgctcgtcgc
     2281 gtgggaggag gcgcaccgtg gactccgtta tagggtcggg aggaggggtg ccggaaaata
     2341 ccacaccctc cgcgcggcac cagtcgccgg cgttcgccga tccgcccgtc aggccgtggc
     2401 gcgacggtcg aggctccgat actggatggc ctcgcgcacg tgcggcggcg cgaggtctgc
     2461 cgcgccatcg aggtcggcga tggtccgggc gagcttgagc acccggtgat aggcccgcgc
     2521 cgagagcccg agccgggtga tggccgaccg gaggagcccc tccacgtcct cgcccacccg
     2581 gcaccaccgc cgcaggtcac gcgcgcccat ctgcgcgttg gcatggatgc cggggcgccc
     2641 gcggaaccgc tcgcgctgac gcgcccgcgc ctgctccacc cggacgcgga tgtccgcgct
     2701 cgactcgacc gccgcctcgc ttgagaggtc gcgatagttg accgccggca cctcgaggtg
     2761 gatgtcgatg cggtcgagga gggggcccga gatccgggcc aggtaccgct ccaccgcctc
     2821 cggggggcag tgacaggagt gggtgggatc gccgtgatag ccgcaggggc agggattcat
     2881 cgcggctgcg aggatgaatc gtgccggata ggtgaggctc accgccgccc ggctcagtgt
     2941 cacgtgcccg tcctcgagcg gctggcgcag cacctcgagg acgttcttgc ggaattccgg
     3001 gagctcgtcg aggaacagca ccccgccatg ggccaggctc acctccccgg gccgtgggtt
     3061 ggacccgccg ccgatgaggc cggcgtcgct gatggtgtgg tgcggcgccc ggaacgggcg
     3121 gatcacgctc aatgactgcc cgggcgccag gagaccggcc acgctgtgga tcttggtggt
     3181 ctcgagcgcc tcatccaggt ccatcggggg caggatcgtc ggcagccggc gcgcgagcat
     3241 cgtcttcccc gccccgggcg gcccgatgag caggatgttg tgcccgccgg cggccgccac
     3301 ctcgagtgcc cgtttggcgg acacctgcgc cttgacatca ttgaaatcca ccacatcctg
     3361 gtgccgctcg gccatcagcg ccgcgaggtc gacgtgcgcg accgccagct ccgcctcgcc
     3421 ggtcaggtgg cggcacacgt cgagaagcgt ccgcgctccc cgcaccgcca gcccatcgac
     3481 cactgccgcc tcccggagat tctgctccgg caggacgatg ccgcggcacc ccgccgcgcg
     3541 ggcggcgagc gccatcgaca gcgcaccccg cacgggtcgg aggtccccct ccagcccgag
     3601 ttccccgagg aacacctccc ctttgagcgc atcctcgggc acctgccccc cggcggcgag
     3661 caccccgagc gcgatcggca ggtcgagcgc cgagccatcc ttgcgaacgt cggccggggc
     3721 caggttgacg gtcaccttct tgagggggac cgagtacccg gcgttggcca gggccggaaa
     3781 gacccgttcc cgcccctccc gcaccgcacc gtgcgggagc ccgaccacgg agaacgacgg
     3841 cagtccattg gtgatgtcga cctcgacgtc gacaaggtag gcgtcgacgc cgagcaccgc
     3901 agtggcctgg acacggctga gcatgcgcca tcccggtcgg agatcgccac agggtacacc
     3961 gggacggcgg cgggcgcggc accctttccg cgccgcacag cgccgatttc ccgcaggcga
     4021 acgcgggtcg gccgactatg aagcaacctt cgcctccgat ttcagaaacc ctcacccccg
     4081 tgcactagaa tgaggcactc acccggcaca ctcggcgccg gtgctgacga agccatcgcc
     4141 ctttgggaga ggatgacatg cgcagtcccc gcttggtcgc actcctgttc gccatgatcg
     4201 ccggcgccgc cggcgcgtat ttc
//
LOCUS       DASUAP010000100         4808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_861261, whole genome shotgun sequence.
ACCESSION   DASUAP010000100 DASUAP010000000
VERSION     DASUAP010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861261"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..844)
                     /locus_tag="VFU45_02515"
     CDS             complement(209..844)
                     /locus_tag="VFU45_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU5357967.1"
                     /translation="MVSRGGVVSTAVHLLVIAGAIQFGRAPDVGSTRIQGPAIAVDAS
                     FVGSTGGEAGPRASGPSPRPVRLDLQPIGVHLEGGFDPGRLAAGRLDPRSFVPRCDSG
                     CDRRIVAVAGPLEGMDAGSDSPPKLVFAPPARYPEELRQGNVRGAVAVEFVVDRSGRV
                     ERGSLSVRTATDDRFIAPALESVAGERFTPGRLGGVPARTRVRQVVMFDVS"
     gene            complement(940..2406)
                     /locus_tag="VFU45_02520"
     CDS             complement(940..2406)
                     /locus_tag="VFU45_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5357968.1"
                     /translation="MTTIRGTLTVWFMLALGLALAAMGAALYVDRKQSVAVEVDAQLT
                     KEVGFARRWLSESHRVLGRVVTTDSVPALVQQVGIYFEPFQDYLLVVNPQGRILFMSE
                     EARSLSYDALQELQTPLTPLPGADLTGTVSLPGNDLRYRVAPVNDAGSDVAAVLVAVP
                     REQVTYGPADLLRSMLILTPLVLLLALGLGWWLAGRAVAPLGPMLDEIQAVTDGRSLH
                     RRVAVPPTGDELARLATKVNEMLSRLEQSFSSLRRFTADASHELKTPLQVLRAGVERA
                     LTHPGAPPESIEALDETLNQINAMSELVESLLTLARADEGAVTLAVEPCDLRDVLADV
                     VETAGLLGDQSGVKVASRAPTAPVVVPVDRPRIRQMLMNLATNAVKYTPKGGRVDLEL
                     SEAGRHVTLVVRDNGIGIAPGDLPHIFDRFWRADVARSRTGERAGAGLGLAITKWIAE
                     AHGGNIAVQSRPGRGTVFTVTLPKDGVPLSEIPAPVEV"
     gene            complement(2547..3215)
                     /locus_tag="VFU45_02525"
     CDS             complement(2547..3215)
                     /locus_tag="VFU45_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5357969.1"
                     /translation="MKILVIEDDRTVGQYVRRGLEEQRYQVELVDDGTEGLKAASTGQ
                     YDLVVLDLRLPGMTGLEILRTLRDRGVTVPILVLTAQDSVEFKVQALRAGADDYVTKP
                     FAFEELLARVEAVSRRPKQVAPPVLTVADLTLDTGTREVHRGGAPVELTPKEYLVLEY
                     LMRHHGRVMSRTLITEYAWDYHFDPGTNIVDVVINRLRKKVDSGHDRRLIHTVRGVGY
                     VVRV"
     gene            complement(3212..4762)
                     /locus_tag="VFU45_02530"
     CDS             complement(3212..4762)
                     /locus_tag="VFU45_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5357970.1"
                     /translation="MTETAVAPIRGPTLVGAVAKLLSAGHPPEDTLTGVADLLYRGIP
                     ARVVTLWVRDASATQFRAITSPPATPAPRTSAQLQALAHDGSLAATRVRLPLIHEDER
                     LGLLEVEADGALASERREILEIVVDIVAPFLASLELSKDLAQEVALRAREIEEQRQFI
                     SQVIDSLPVGLYVVDRDYRVTLWNRMRELGTQGLRREEVVGRTVFDVLSRQNAETLRH
                     EFDLAFRTGELQQMELETQVDGEPRHYRISKIPMRASDGHVSHVITIGEDVTDWRTVQ
                     SRIMQSEKLAAVGQLAAGVMHEINNPLATIGACVEAIGHRIADGGPQAVASIREYVDI
                     IDKEVQRCTGIVDGLLDFSRPKATARRTVNLTAVLEDTLFLLKHHQRFKRIPITLELA
                     QDLPAMQANGEQLVQVFMALMLNAVDAMEGRAGTLTVRTFRNPDRADEVVAEVHDTGV
                     GMSQAEISKIFEPFYTTKLPGRGTGLGLSICYGIVQQHHGRIAVDSTPGAGTTFRVFL
                     PLGARAAG"
BASE COUNT          707 a         1689 c         1714 g          698 t
ORIGIN      
        1 ttcttcgact cgatcaggtt atcgaacacc gatcctccgg tggatgattg acgcctgcgg
       61 gatgaatgct accgaccccg ccttaccgcc gtctgggccg aagattacaa gacgatatca
      121 actggatgat ggtcggggaa cagctatcag ctatcagcta tcagctatca gctatcagcg
      181 gccgaaggtc gcccgcccga gcgcgccgtc aagagacgtc gaacatcacg acctgccgca
      241 cacgggtgcg ggctggaaca ccccccagcc tcccgggggt gaaccgttcg ccggcgaccg
      301 attcgagggc gggtgctatg aagcgatcat ccgtggccgt ccgaacggac agcgatccgc
      361 gttccaccct cccggatcgg tcgaccacga actcgacggc gaccgcacct cggacattac
      421 cctgccgcaa ttcttccggg taccgggccg gaggcgcgaa caccagcttg ggcggcgagt
      481 ccgatcctgc gtccatgccc tcgagcggcc cggccacggc cacgatccgg cgatcgcaac
      541 cggaatcaca gcgcggcacg aacgaacggg ggtcgaggcg cccagccgcc aggcgaccgg
      601 gatcgaaccc gccttcgagg tggaccccga tcggctgaag atcgagccgc accggccgag
      661 gcgatggccc gctggcacgc ggcccggcct ccccgcctgt cgagccgacg aaggacgcat
      721 cgaccgcgat cgccggccct tggatgcgag tgctgccgac gtccggggcc cgcccgaact
      781 ggatcgcccc ggcaatgacc agcaggtgca ccgcagtgga caccacgccg ccgcgcgaga
      841 ccatcacgac ctccagtgag ggtgatggtc cacgctatct gccgggcggt ccccccgccc
      901 gacggtcgga tgacggcccg atgacattcc gatgccgggt cagacttcga ccggcgccgg
      961 gatctcggag agcggcacgc catccttggg gagggtcacg gtgaagaccg tcccccgccc
     1021 cggccggctc tgcacggcga tgttgccgcc gtgcgcctcg gcgatccact tggtgatcgc
     1081 gaggccgagc cccgcgcccg cgcgctcgcc ggtccgggag cgcgccacgt cggcgcgcca
     1141 gaaccggtcg aagatgtgcg gcaggtcgcc cggcgcgatg ccgatgccgt tgtcgcgcac
     1201 gacgagcgtc acgtgccgcc ccgcctcgga gagctcgagg tccacccggc cccccttggg
     1261 cgtgtacttg accgcgttgg tggccaggtt catgagcatc tgccggatgc gcggccggtc
     1321 caccggcacc acgaccggtg ccgtcggggc ccggctcgcc accttgaccc ccgactggtc
     1381 gccgaggagg cccgcggtct ccaccacgtc ggccaggaca tcgcggaggt cgcacggctc
     1441 gaccgcgagc gtcaccgccc cctcgtccgc gcgggcgagg gtgagcaggc tctccaccag
     1501 ttcgctcatc gcgttgatct ggttgagggt ctcgtcgagg gcctcgatgc tctccggcgg
     1561 ggcgcccgga tgggtgagcg cccgttcgac gccggcgcgg agcacctgca gcggggtctt
     1621 gagctcgtgg ctcgcgtcgg cggtgaagcg gcggagcgag gagaagctct gttccagccg
     1681 ggagagcatc tcattgacct tggtggcgag ccgtgcgagc tcgtcgccgg tgggcggcac
     1741 cgccacccgc cggtggaggc tccggccgtc ggtgaccgcc tggatctcgt cgagcatcgg
     1801 cccgagcggc gccaccgccc ggccggcgag ccaccacccg agcccgagcg ccaggagcag
     1861 cacgaggggg gtgaggatca gcatcgagcg aaggaggtcg gcggggccgt acgtcacctg
     1921 ttcgcgcggc acggccacga gcaccgccgc cacgtcggaa ccggcgtcgt tgaccggggc
     1981 cacgcggtag cgcaggtcgt tgccggggag ggagacggtc ccggtcaggt cggcccccgg
     2041 gagcggcgtg agcggcgtct ggagctcctg cagcgcgtcg tagctcaggc tccgcgcctc
     2101 ctcggacatg aagaggatcc gcccctgcgg gttgaccacg aggaggtagt cctggaacgg
     2161 ctcgaagtag atgcccacct gctgcaccag ggccggcacg gaatcggtgg tgaccacccg
     2221 cccgagcacc cggtgcgact cgctcagcca ccggcgggcg aacccgacct ccttggtgag
     2281 ctgggcgtcc acctcgaccg ccaccgactg cttgcggtcg acgtagaggg ccgcccccat
     2341 cgccgcgagg gcgaggccga gggcgagcat gaaccagacg gtgagggtgc cgcggatcgt
     2401 ggtcatcggg agggtccggg cccggcgcgg gagggcgccg ggtcgttcag agttggcctg
     2461 cgtcgggagc agggaagtct atcgcgataa cgggaacgca caactggcgg cgggacgcac
     2521 acggaccggg gcgccggccc tgggcgctac acccgcacga cgtagcccac gccgcgcacg
     2581 gtgtggatga gccggcggtc gtggccgctg tccaccttct tccggaggcg gttgatgacc
     2641 acgtcgacga tgttggtgcc cggatcgaag tggtagtccc aggcatattc ggtgatgagc
     2701 gtccgggaca tcacccgccc gtggtgccgc atcaggtact cgaggacgag gtactccttc
     2761 ggggtgagct ccaccggcgc cccgccccga tggacctcgc gggtcccggt gtcgagggtc
     2821 aggtcggcca cggtgagcac cggcggcgcc acctgcttgg ggcgccggct caccgcctcg
     2881 acccgggcca ggagttcctc gaaggcgaac ggcttggtga cgtagtcgtc ggcaccggcg
     2941 cggagcgcct gcaccttgaa ctccaccgaa tcctgggcgg tgagcaccag gatcggcacc
     3001 gtgacgccgc ggtcgcggag cgtccgcagg atctcgagcc ccgtcatccc cgggagccgg
     3061 aggtcgagca ccacgaggtc gtactgcccg gtcgacgcgg ccttgagccc ctcggtgccg
     3121 tcgtccacca gttccacctg gtagcgctgc tcctcgaggc cccggcgcac gtactggccg
     3181 acggtgcggt cgtcctcgat gacgaggatc ttcatcccgc cgcccgggcg ccgaggggga
     3241 ggaagacccg gaaggtggtc cccgccccgg gggtgctgtc caccgcgatg cgcccgtggt
     3301 gctgctgcac gatgccgtag cagatcgaga gcccgagccc ggtgccgcgc cccgggagct
     3361 tggtggtgta gaacggctcg aagatcttgg agatctccgc ctgcgacatc ccgacgccgg
     3421 tgtcgtgcac ctcggcgacg acctcgtccg cgcggtcggg gttccggaag gtccgcaccg
     3481 tgagcgtgcc cgcccgcccc tccatggcgt ccaccgcgtt cagcatcagc gccatgaaca
     3541 cctgcaccag ctgctcgcca ttggcctgca tggccggcag gtcctgcgcc agctccaagg
     3601 tgatcgggat gcgcttgaag cgctgatggt gcttgaggag gaagagggtg tcctccagca
     3661 ccgccgtcag gttgaccgtc cgccgcgcgg tggccttggg gcggctgaag tcgaggagcc
     3721 cgtcgacgat gccggtgcag cgctgcactt ccttgtcgat gatgtcgacg tactcccgga
     3781 tcgacgccac cgcctggggg ccgccgtccg cgatccggtg gccgatggcc tcgacgcagg
     3841 ccccgatcgt ggcgagcggg ttgttgatct cgtgcatcac ccccgccgcc agctgcccca
     3901 cggccgccag cttctcgctc tgcatgatcc ggctctgcac cgtgcgccag tcggtcacgt
     3961 cctcgccgat ggtgatcaca tgcgagacgt gcccgtcgga ggcgcgcatc gggatcttgg
     4021 agatgcggta gtggcggggc tcgccgtcga cctgggtctc gagctccatc tgctgcagct
     4081 cgccggtgcg gaaggcgagg tcgaactcgt gccgcagggt ctcggcgttc tgccggctca
     4141 gcacatcgaa caccgtgcgg ccgacgacct cctcccggcg cagcccctgg gtgcccagct
     4201 cgcgcatgcg gttccagagc gtcacccggt agtcgcggtc gaccacgtag agcccgaccg
     4261 ggagcgagtc gatgacctgg ctgatgaact ggcgctgctc ctcgatctcg cgggcccgga
     4321 gcgccacctc ctgggcgagg tccttcgaca gctccaggga cgcgaggaac ggcgccacga
     4381 tgtcgacgac gatctcgagg atctcgcgcc gctcggacgc gagggcgccg tcggcctcga
     4441 cctcgaggag gccgagccgc tcgtcctcgt ggatgagcgg caggcggacc cgcgtggccg
     4501 cgagcgaacc gtcgtgggcc agcgcctgca gctgggcgct ggtccgcggc gccggggtgg
     4561 ccgggggcga ggtgatggcc cggaactggg tggcgctggc atcgcgcacc cagagcgtca
     4621 ccacccgcgc cgggatgccc cggtacagca ggtcggcgac gccggtgagg gtgtcctccg
     4681 gcgggtgccc ggcgctcagc agcttggcca ccgcgcccac gagcgtgggt ccgcggatcg
     4741 gcgcaacggc ggtctcggtc atttgaatcc cgtgagctgg atcccgcgga tgaagtaacg
     4801 ctgcgccg
//
LOCUS       DASUAP010000101         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_193267, whole genome shotgun sequence.
ACCESSION   DASUAP010000101 DASUAP010000000
VERSION     DASUAP010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193267"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFU45_02535"
     CDS             complement(<1..186)
                     /locus_tag="VFU45_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HEU5357971.1"
                     /translation="MTLLLSIHDVTPAFESEVRALHAMCARIGAEPALLVVPDWHGEW
                     PLDRHPAFVDGVRGRAAE"
     gene            complement(183..1460)
                     /locus_tag="VFU45_02540"
     CDS             complement(183..1460)
                     /locus_tag="VFU45_02540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5357972.1"
                     /translation="MTAPTDRRSSGIAAVAAALVPAGDLRGRAFDLAALPPVERAGAE
                     LAILDVTKYFGDTSGGIRTYLLEKARYVSARTALRQVLVLPGARRALTGIDGVRCYRL
                     RGPAIPFQHPYRFLVGPRALRRIVAHERPDLIEVGSPFAVPWMARRANRGLGAAMVWF
                     YHGNIPRVIAPRPAQASASRRRLAELAWRYVRRVGGLFDVTFAGSAFAARDLETHGVP
                     NVVRVPLGVDLELFTPERRVHRDLVRRRERWPEGPLAIHIGRLAGEKELEVVLRAWPE
                     VERRTGATLVVVGAGPSAGRYRRLARGRILWRGFEHDRAALADLIAAADLYVSPCPIE
                     TFGLAALEAFASGVPVLSAGEGGVAEKVTASGAGATYPPGEVAACAEAAISLFRDDLP
                     SLGRVGRAYAEEHHAWPRALDAIFAEYRRVLAR"
     gene            complement(1625..2269)
                     /locus_tag="VFU45_02545"
     CDS             complement(1625..2269)
                     /locus_tag="VFU45_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5357973.1"
                     /translation="MSDLVVQTIPNGRYVENCYLLGDPDAGTAVLIDPGEEPARALAA
                     LRHRGWTLDAIWLTHGHVDHVLGAGEVKAATGAPIRLHSADRFLYDAAPHFSAEPWRP
                     VEMPSPDAGWRDGEVVRVGRFPFTVRHVPGHSPGHVCFVGADAVFAGDVLFGGSIGRT
                     DLPGSDHATLLESIHRVLLALPDSTTVFPGHGRPTTIGVERETNPFLGAVRHGG"
     gene            complement(2262..3218)
                     /gene="trxB"
                     /locus_tag="VFU45_02550"
     CDS             complement(2262..3218)
                     /gene="trxB"
                     /locus_tag="VFU45_02550"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU5357974.1"
                     /translation="MSDATTQEFDTIIIGGGPAGLCAAMYAGRGMLNAVLLERGVPGG
                     ELLNTEVIEDYPGFEHILGRDLAAKMNAHALKFGANIRQETVESVEKQDDGSFTVATS
                     SGATYRAPTVILTAGGTPTKLGVPGELEYAGRGVSYCAVCDGAFFKGETIAVVGGGDA
                     AVEEADYLTRYAAKVYLIHRRDELRASRILQDRLFANPKIEVIWNKQVLELAGGPSGL
                     QKLRLQDTLTNAASDLAVTGCFIFVGFKPNSGLIKGHFRHDASGYVITDERMMTSIPG
                     LFAAGDLRVQLTRQVTTAVGDATTAAIAVEKYLAKQRSQAGV"
BASE COUNT          502 a         1194 c         1308 g          429 t
ORIGIN      
        1 ctcggcggcc cggccgcgga ccccgtcgac gaaggccggg tggcggtcga gtggccactc
       61 gccgtgccag tcggggacga ccaggagcgc cggctccgcg ccgatccgcg cgcacatcgc
      121 gtgcagcgcc cgcacctcgg actcgaacgc cggggtgacg tcgtggatgg agaggagcag
      181 ggtcatcgcg ccagcacccg ccgatattcg gcgaagatgg cgtcgagcgc gcggggccac
      241 gcgtggtgct cctccgcgta cgcccggccc acacggccga gcgacggcag gtcgtcgcgg
      301 aagagcgaga tggcggcctc cgcgcacgcc gccacctccc cgggcggata cgtggcgccc
      361 gcgcccgagg ccgtcacctt ctccgccacg ccgccttcgc cggcggagag cacgggcacg
      421 ccgctcgcga aggcctcgag cgcggcgagg ccgaaggtct cgatgggaca aggggacaca
      481 tagaggtcgg cggcggcgat gaggtcggcg agcgcggcgc gatcgtgctc gaacccgcgc
      541 cagaggatgc ggccccgggc caggcgccgg tagcggccgg cggaggggcc ggcgcccacc
      601 accaccagcg tggcgccggt gcggcgctcg acctccggcc aggcgcggag gaccacctcg
      661 agctccttct cccccgcgag gcgcccgatg tggatcgcga gcggcccctc gggccagcgc
      721 tcgcgtcggc gcaccaggtc ccgatgcacc cggcgctccg gcgtgaacag ttcgaggtcg
      781 acgccgagcg gcacgcggac gacgttcggc acgccatggg tttcgaggtc gcgggcggcg
      841 aacgccgatc cggcgaaggt gacatcgaag aggccgccca cccggcggac gtagcgccag
      901 gcgagctcgg ccagtcgccg gcggctcgcc gaggcctgcg cgggccgggg cgcgatgacg
      961 cgcgggatgt tgccgtggta gaaccagacc atcgccgcac cgaggccccg gttggcgcgc
     1021 cgggccatcc acggcaccgc gaacgggctg ccgacctcga tcaggtccgg gcgttcatgc
     1081 gcgacgatcc ggcggagcgc ccggggtccc accaggaacc ggtaggggtg ctggaacggg
     1141 atcgcagggc cgcggagccg gtagcaccgc acgccgtcga tcccggtgag cgcgcggcgg
     1201 gcgcccggca gcaccagcac ctgccgcagc gccgtccgcg cggagacgta ccgcgccttc
     1261 tccaggaggt aggtgcggat gccaccgctc gtgtcgccga agtacttggt gacgtcgagg
     1321 atcgcgagct ccgcgccggc ccgctccacg ggcgggagcg cggcgaggtc gaacgcacgg
     1381 ccccgcaggt cccccgcggg tacgagcgcg gcggccaccg cggcgatgcc ggaggagcgc
     1441 cggtcagtcg gagcagtcac caagggggta cacggtgccg cagttggggc acggattctt
     1501 gcatccccag gcccgggaga tgacggggaa tccgcagcga ttgcaggtga gcgggtcgga
     1561 cggatggatg gacggacggt cggatgacgg cagcggcgcg ggcggggcgt cggggcgacg
     1621 cgattcaccc gccatggcgc accgcgccca ggaacgggtt ggtctcccgc tcgacgccga
     1681 tggtggtggg gcgcccgtgc cccggaaaga ccgtcgtgct gtcgggcagc gcgaggagca
     1741 cccgatggat gctctcgagg agcgtggcgt ggtcgctgcc ggggaggtcg gtgcggccga
     1801 tgctgccgcc gaagagcaca tcgccagcga agacggcgtc ggccccgacg aagcagacgt
     1861 ggcccggcga gtgccccggc acgtggcgca ccgtgaacgg gaagcggccg acccgcacga
     1921 cctcgccatc ccgccagccc gcgtcgggcg acggcatctc caccgggcgc cacggctccg
     1981 cggagaagtg cggcgcggcg tcgtacagga aacggtcggc cgagtggagg cggatcgggg
     2041 cgccagtggc cgccttgacc tcaccggcac cgagcacgtg gtccacgtgg ccgtgggtga
     2101 gccagatcgc gtcgagggtc cagccccggt gccggagcgc cgcgagcgca cgggcgggct
     2161 cctcgcccgg gtcgatcagg acggccgtgc cggcgtcggg atcgccgagg aggtagcagt
     2221 tctcgacgta gcggccgttg gggatcgtct ggacgacgag gtcagacacc ggcctggctc
     2281 cgctgcttgg ccaggtactt ctcgaccgcg atcgcggcgg tggtggcgtc gccgacggcg
     2341 gtggtgacct gccgggtgag ctgcacccgg aggtcgccgg cggcgaagag cccggggatc
     2401 gacgtcatca tccgctcatc cgtgatgacg tagccgctgg catcgtgccg gaaatgaccc
     2461 ttgatgagcc ccgagttcgg cttgaagccg acgaagatga agcacccggt gaccgcgagg
     2521 tccgacgcgg cgttggtcag cgtgtcctgg aggcgcagct tctggagtcc cgatggcccg
     2581 cccgcgagct cgagcacctg cttgttccag atgacctcga tcttcgggtt ggcgaagagc
     2641 cggtcctgca ggatgcgcga ggcgcgaagt tcgtcccgcc ggtggatgag gtagaccttg
     2701 gcggcgtacc gcgtgaggta atcggcctcc tcgaccgcgg cgtcgccgcc gcccaccacg
     2761 gcgatcgtct cgcccttgaa gaaggcgccg tcgcacacgg cgcagtacga cacgccgcgg
     2821 cccgcatact cgagctcacc gggaacgccg agcttggtcg gggtgccgcc ggcggtgagg
     2881 atgaccgtcg gggcccggta ggtggcgccc gaggacgtcg cgaccgtgaa cgagccgtcg
     2941 tcctgcttct ccaccgactc gaccgtctcc tggcggatgt tcgcgccgaa cttgagcgcg
     3001 tgcgcgttca tcttcgcggc gaggtcacgg ccgaggatgt gctcgaaccc gggatagtcc
     3061 tcgatcacct cggtgttgag gagctcgccc cccggcaccc cgcgctcgag gagcacggca
     3121 ttgagcatcc cgcggccggc gtacatcgcc gcgcagaggc cggcgggccc gccgccgatg
     3181 atgatggtgt cgaattcctg cgtcgtcgcg tctgacatag ctcaggccgg cagtgggtca
     3241 gtgggtcagt gggtcagtgg gtcagccgaa agatagtcgc catgcacccg tcccatctaa
     3301 cgccctgcgg ttcccggcgc accgacctcg agcagcgccg cggccccgtg acaggccgag
     3361 acggtggcca cgaggagcat gccgccgcgc ggccccaccg cgtcgcagag catgcgttcg
     3421 gccgcacgcg cac
//
LOCUS       DASUAP010000102         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_302471, whole genome shotgun sequence.
ACCESSION   DASUAP010000102 DASUAP010000000
VERSION     DASUAP010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302471"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..2506)
                     /locus_tag="VFU45_02555"
     CDS             complement(179..2506)
                     /locus_tag="VFU45_02555"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEU5357975.1"
                     /translation="MQFKDEHPPYAWAGVAAAVLLAIYVATLSPTTAFWDTSEYIAAA
                     KVLGIPHPPGNPLFTVVAHTWGLIPWAADYAVRINLFAAVTSALASGLWFLVSERWMR
                     GIVPARWPRYAAAFAGVLAAGTAWTVWNQSVVNEKVYTLSMLSVALVVWLAVHWGDAE
                     PGPARDRWLILVGYVLALTSTNHLMGVLGAPAVAVYVLWTEPRVIFKPWAPLLGWFLA
                     IAVFNHLPDLAHPGAGPTEMLVTGVTLALIVYAIWRDPKDQLAYLAVGAVLVGISLNY
                     VYLPIRAAQYPPINEGEPIGFLSQALSDVLNRVQYGKPSLMDRQAPFYYQMATFLQYF
                     GWQWARNWKAFAGIATGVFAFLGFSGLFGLWRSDRRAAYAAIALLGTLSVALVFYMNF
                     KYGFSIAWQEFGNNVPREVRERDYFFMGAFAVWGVCAGLGIAVWMRTIADFTTRAGTA
                     PARWAVSTPLLALAIIPFFGNRATASRAHETVARDLAVDMLQSVAPYGILITAGDNDT
                     FPLWFAQEVLGVRTDVTLANLSLMNTDWHLRQIKRRETPDFDAANALPYWKDRIKAGD
                     VPGELPGGTITKPVNPVFQWSIPQLDSMADLTPVAKDQHVKWDSLDITINGTYIPQGN
                     GYYLEKSDLATLMLIHDNLGKRPIYFSITDAFHPDQVFGLRPYLVAEGLARRLYPAPV
                     KPGPNIVTSPYIGYLDVNATDKLIWDVYHYQAVTRKRPYGWVDPPSGSILTVYGLIYA
                     SMAQVYADKGDSAKARKAEQVYEGIAAQMPANGPR"
     gene            complement(2601..>3652)
                     /gene="purH"
                     /locus_tag="VFU45_02560"
     CDS             complement(2601..>3652)
                     /gene="purH"
                     /locus_tag="VFU45_02560"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU5357976.1"
                     /translation="VSREERRALAAKVFAHTGDYDAAIANYLAAHDTELPAVLAVSLR
                     RSQALRYGENPGQRAALYVTEEPRGMADLTQRQGKELSFNNLLDIEAATSAAALWSNR
                     PACVVIKHTTPCGIAVGTSSEHAFRKARATDPLSAFGSVVGFNTVVSAAAAQAMADLF
                     VEVVVAPSFQAEALEIFKAKKNLRVVELPVGGSNRGLDFKRVRGGFLVQDRFVYDGDE
                     SAWKVVTQRQPTDPERGDLAFAWAAVGAVKSNAILIARDEAAIGIGAGQMSRVDSAFL
                     AVHKAHKEGHDTKGAVLASDAFFPFPDGVEEAAAAGITAIIQPGGSVRDAEVFAAADR
                     LGIAMIVTGKRQFRH"
BASE COUNT          571 a         1224 c         1317 g          540 t
ORIGIN      
        1 agccatcggc gccggcggac gaactcctcc tccatcagcg ccacatgcgc cgccggtccg
       61 ccgaacgcag tagcgccgag ccggaggaag atcccgagca cctcggccgc gccgccgcct
      121 gaagacgaaa gatcccccgg agcatcactg ccccggggga tctcgtggga cggcacggct
      181 accgcggccc gttcgcgggc atctgcgccg cgatcccctc gtacacctgc tccgccttcc
      241 gggccttggc ggaatcaccc ttgtcggcgt acacctgggc catcgaggcg tagatcaggc
      301 cgtacacggt gaggatcgac ccggacggcg ggtcgaccca cccgtagggg cgcttccggg
      361 tcaccgcctg gtagtggtac acgtcccaga tcagcttgtc tgtggcgttc acgtccagat
      421 agccgatgta cggcgacgtc acgatgttgg ggcccggctt gaccggcgcg gggtagagcc
      481 ggcgcgccag cccctcggcc acgaggtagg gccggagccc gaagacctgg tcggggtgga
      541 aggcgtcggt gatcgagaag tagatcggcc gcttgcccaa gttgtcgtgg atcagcatca
      601 gggtggccag gtcgctcttc tcgaggtagt agccgttccc ctgcgggatg taggtgccgt
      661 tgatggtgat gtcgagcgag tcccacttga cgtgctggtc cttggccacc ggcgtcaggt
      721 ccgccatcga gtcgagctgg gggatgctcc actggaacac cgggttgacc ggcttggtga
      781 tggtgccgcc cggaagttca ccggggacgt cgccggcctt gatgcggtcc ttccagtagg
      841 ggagggcgtt ggccgcgtcg aagtcggggg tctcccgccg cttgatctgg cgcaggtgcc
      901 agtcggtgtt catcagcgac aggttggcga gcgtcacgtc ggtgcgcacc cccagcacct
      961 cctgcgcgaa ccagagcggg aaggtgtcgt tgtcgccggc ggtgatcagg atgccgtatg
     1021 gggccaccga ctgcagcatg tcgaccgcga ggtcgcgggc cacggtctcg tgggcccggc
     1081 tggcggtggc gcgattgccg aagaacggga tgatcgccag cgccagcagg ggcgtcgaca
     1141 cggcccagcg ggcgggggcg gtcccggcgc gggtggtgaa gtcggcgatg gtgcgcatcc
     1201 agaccgcgat gcccaggccg gcgcacaccc cccacaccgc aaaggcgccc atgaagaagt
     1261 agtcgcgctc gcgcacctcg cgcggcacgt tgttgccgaa ctcctgccac gcgatcgaga
     1321 acccatactt gaagttcatg tagaacacga gcgccacgct gagcgtgccg aggagcgcga
     1381 tcgcggcgta cgccgcacgg cggtccgacc gccagagccc gaagaggccg gagaagccca
     1441 ggaacgcgaa gaccccggtc gcgatgccgg cgaacgcctt ccagttgcgg gcccactgcc
     1501 agccgaagta ctggaggaag gtggccatct ggtagtagaa cggggcctgg cggtccatca
     1561 gcgacggctt cccgtactgg acgcggttga gcacgtcgct cagggcctgg ctcaggaagc
     1621 cgatcggctc gccctcgttg atcggcggat actgggccgc gcggatcggg aggtagacgt
     1681 aattcagcga gatgccgacg agcacggcgc cgacggccag gtaggccagc tggtccttgg
     1741 ggtcgcgcca gatggcgtag acgatgaggg ccagggtgac gccggtgacc agcatctccg
     1801 tggggccggc gccggggtgg gccaggtcgg ggaggtggtt gaacaccgcg atggcgagga
     1861 accagccgag gagcggtgcc cacggcttga agatgacccg gggctcggtc cagagcacgt
     1921 acaccgccac cgccggcgcg ccgaggacgc ccatcaggtg gttggtggaa gtgagggcca
     1981 ggacgtagcc cacgaggatg agccaccggt cgcgcgcggg gcccggttcg gcgtcgcccc
     2041 agtggacggc gagccagacg acgagcgcca ccgacagcat cgagagggtg tacaccttct
     2101 cgttgaccac cgactggttc cacaccgtcc acgccgtgcc cgccgccagc accccggcga
     2161 aggcggcggc gtagcgcggc caccgggccg gcacgatgcc ccgcatccac cgctccgaca
     2221 ccaggaacca gagccccgag gccagggcgc tggtgacggc ggcgaagagg ttgatccgca
     2281 ccgcgtagtc ggccgcccag gggatgagcc cccaggtgtg ggccaccacc gtgaagagcg
     2341 ggttgcccgg cggatgcggg atcccgagca ccttcgcggc ggcgatgtat tccgaggtgt
     2401 cccagaacgc cgtcgtgggc gagagcgtcg ccacgtagat ggcgaggagg accgcggcgg
     2461 ccacgccggc ccaggcatag ggagggtgct cgtccttgaa ttgcatgcgt gtcgatccct
     2521 ggtgaacggt caacggtgaa cggtcaacgg gggtgtacgg ggcggtcccg ttcacccttc
     2581 accgttgacc gtttacgctg tcaatgcctg aactgcctct tgcccgtgac gatcatggcg
     2641 atgccgagcc ggtcggcagc ggcgaagacc tcggcgtcgc gcaccgagcc gccgggctgg
     2701 atgatggcgg tgatccccgc ggccgccgct tcctccaccc cgtcggggaa ggggaagaac
     2761 gcatccgacg cgaggacggc gcccttggtg tcgtgccctt ccttgtgcgc cttgtggacg
     2821 gcgaggaacg cgctgtccac ccggctcatc tggccggcgc cgatgccgat ggcggcctcg
     2881 tcccgcgcaa tgaggatggc gttcgacttg accgccccca cggccgccca ggcgaaggcc
     2941 aggtcgccgc gctcgggatc ggtcggctgg cgctgggtca ccaccttcca ggcggactcg
     3001 tcgccgtcgt agacgaaccg gtcctgcacc aggaatccgc cccgcacccg cttgaagtcg
     3061 agcccgcggt tgctgccgcc gaccgggagc tcgacgaccc gcaggttctt cttcgccttg
     3121 aagatctcca gcgcctccgc ctggaagctc ggcgccacca ccacctcgac gaagaggtcg
     3181 gccatcgcct gggcggcggc ggcgctcacc acggtgttga agccgaccac gctcccgaac
     3241 gccgagagcg ggtcggtggc gcgggccttc cggaaggcgt gctcgctgct cgtgcccacg
     3301 gcgatcccgc agggcgtcgt atgcttgatg accacgcacg ccggacggtt gctccagagc
     3361 gccgccgcgg acgtggccgc ctcgatgtcg aggaggttgt tgaaggagag ttccttccct
     3421 tggcgctggg tgaggtccgc catcccgcgc ggctcctcgg tgacgtagag cgccgcccgc
     3481 tgccccgggt tctccccgta gcggagcgcc tgcgaccgcc ggagcgacac cgccagcacc
     3541 gccggcagtt cggtgtcatg ggccgcgaga tagttggcga tggcggcgtc gtagtcgccg
     3601 gtgtgggcaa agaccttggc cgcgagcgcc cgccgctcct cccgggacac cg
//
LOCUS       DASUAP010000103         3565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1011186, whole genome shotgun sequence.
ACCESSION   DASUAP010000103 DASUAP010000000
VERSION     DASUAP010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011186"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VFU45_02565"
     CDS             complement(<1..975)
                     /locus_tag="VFU45_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5357977.1"
                     /translation="MSAPLDPAAVSALRHDLRTPVNHIVGYCELLLDDAATDEQRGAL
                     QAAIASARDVLALIEAALPPSGAAVTPERVDELRSRLRTPHDRILAAMDTLGPAAGLA
                     ADVAKVRHATGQLLAIDRGSIAHPAAAAPSPPAASALPAAPRGRILVVDDVEDNRTVL
                     RRHLTRQGYTADEAPDGESALRMARNTAYDLMLLDVRMPGIDGLEVLVRTKRDPATRD
                     LPVVMISAADELSTIAGCIEAGADDFLPKPFDPVILGARVSASVEKKRLRDLEVDYLR
                     QVDRVVQAAAAVEKGTYAPGALAEVGRRDDAIGHLARVFDAMAAGVRAR"
     gene            complement(972..1349)
                     /locus_tag="VFU45_02570"
     CDS             complement(972..1349)
                     /locus_tag="VFU45_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5357978.1"
                     /translation="MPRVLLVEDNEMNRDMLSRRLLKAGYEVTMAFDGAQGVEMARAG
                     GHDIILMDMSLPVIDGWEATRQIRAIDALRGVPIIALTAHAMAGDRDKALEAGCSDYD
                     TKPVELPRLLAKMAALLGQAPPA"
     gene            complement(1349..>3565)
                     /locus_tag="VFU45_02575"
     CDS             complement(1349..>3565)
                     /locus_tag="VFU45_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5357979.1"
                     /translation="DSTGTRLALGEGAMGQVAVSHEPLIIPNYDEWAGRSTKYSDVRD
                     TVQAVMVAPLLIGPRLVGAIASTHADPTRHFGADDLRLLQLFAPQAAIAIENARLYTA
                     AQREREFFGAVVANSPVAIVTLETDGRITALNPAFEGLFGYTREEALGRDLDGLISDE
                     RTRSEANAYTSQASQGAIRGIGKRRRKDGSLIDVEISAVPVAIEGRNIGILALYHDVT
                     DLLEAKREAESANQAKSRFLANMSHELRTPLNAIIGYSEMLKEDADDQGNGAWSADLE
                     KIRSSGKHLLALINDVLDLSKVEAGKMELYLETFDVRPLVEEVRTTIQPLVVARGNRL
                     DVRVAAELGPLRADLTKVRQMLLNLLSNATKFTERGSISLDVSRTAAGGGGHDVVFRV
                     TDQGIGMTPEQQSRIFEAFSQAEASTTRRFGGTGLGLAITRRFCEMMGGSIDVESTPG
                     VGSTFTVRLPVDVGSAAEGAAPVPAGEAVTGGGAGRVLVIDDEAPVRALMRRFLAREG
                     FSVDEAADGAEGLARARERRPDAITLDVMMPDMDGWEVLARLKDDPTLADIPVILLTI
                     VDDKKVGYALGATEYLTKPIDREALRRALAPYRHAPGAHRLLLVEDDPTTAELVRRTL
                     ETEGWTVDWARNGREGLARVDAAPPALILLDLMMPEMDGFEFLETLRGTARRNETPVI
                     VVTAKDLTAEDHRRLNGHVSAVLQKGSYSRDELLSEIASRLGHRLRRPASAPTTRA"
BASE COUNT          450 a         1229 c         1359 g          527 t
ORIGIN      
        1 ccgcgcccgg acccccgccg ccatcgcatc gaacacccgc gccaggtggc cgatcgcgtc
       61 gtcccgccgt cccacctcgg cgagggcgcc gggagcgtac gtccccttct cgaccgccgc
      121 ggccgcttgg acgacgcgat ccacctgccg caggtagtcc acctcgaggt cgcgcagccg
      181 cttcttctcc accgacgcgc tcacccgcgc gccgaggatc acggggtcga agggcttggg
      241 aaggaagtcg tcggcgccgg cctcgatgca cccggcgatg gtggagagct cgtcggcggc
      301 ggagatcatc accaccggga ggtcgcgggt ggcgggatcg cgcttggtcc ggaccaggac
      361 ctcgaggccg tcgatccccg gcatgcgcac gtcgaggagc atcaggtcgt aggcggtgtt
      421 ccgcgccatc cggagcgccg actcgccgtc gggcgcctca tcggcggtgt agccctgccg
      481 ggtcaggtgc cggcggagga cggtgcggtt gtcctcgacg tcgtccacga cgaggatgcg
      541 gccccgaggg gcggcgggca gggcggacgc ggcgggcggg gaaggtgcag ctgcggcggg
      601 atgagcgatc gacccgcggt cgatggccag gagctggccg gtggcgtggc gcaccttggc
      661 gacgtccgcc gccaggccgg cggccgggcc gagggtgtcc atcgcggcga ggatccggtc
      721 gtgcggggtc cggaggcgcg accggagctc gtccacccgc tccggcgtca cggcggcgcc
      781 gctcggcggg agcgcggcct cgatgagggc gagcacgtcg cgcgccgacg cgatcgcggc
      841 ctgcagggca ccgcgctgct cgtcggtggc cgcatcgtcg agcaggagct cgcagtagcc
      901 cacaatgtgg ttgaccgggg ttcgcaggtc gtggcggagg gccgacaccg ccgccgggtc
      961 gaggggggcg ctcacgcggg cggggcctgg ccgaggaggg cggccatctt ggccaggagg
     1021 cgggggagct cgaccggctt ggtgtcgtag tcgctgcacc ccgcctcgag cgccttgtcg
     1081 cggtcgcccg ccatcgcgtg ggcggtgagc gcgatgatcg gcaccccgcg gagcgcgtcg
     1141 atggcgcgga tctgccgggt ggcctcccag ccgtcgatca cgggaaggct catgtccatc
     1201 aggatgatgt cgtgcccgcc ggcccgcgcc atctcgacgc cctgcgctcc gtcgaaggcc
     1261 atcgtcacct cgtagcccgc cttgaggagc cggcgcgaga gcatgtcgcg gttcatctcg
     1321 ttgtcctcga ccagcagcac gcgcggcatc aggccctcgt cgtgggtgcg gaggctgggc
     1381 gccggagccg atggcccagg cgggaggcga tctccgagag cagctcgtcg cgggagtaac
     1441 tccccttctg cagcacggcg ctcacgtggc cgttgaggcg gcggtggtcc tcggcggtga
     1501 ggtccttggc ggtcaccacg atgaccggcg tctcgttccg gcgcgcggtg ccgcgcaggg
     1561 tctccaggaa ctcgaagccg tccatctcgg gcatcatcag gtcgagcagg atgagcgccg
     1621 gcggcgcggc gtccacccgg gccagcccct cgcggccgtt ccgtgcccag tccacggtcc
     1681 atccctctgt ctcgagggtc cgccgcacca gctcggcggt ggtcgggtcg tcctccacca
     1741 gcaggagccg gtgggcgccg ggggcgtggc ggtagggcgc gagcgcgcgg cggagcgcct
     1801 cgcgatcgat cggcttggtc aggtactcgg tggcgccgag ggcgtagccc accttcttgt
     1861 cgtcgacgat ggtgaggagg atgaccggga tgtcggcgag tgtcgggtcg tccttgaggc
     1921 gcgcgagcac ctcccagccg tccatgtccg gcatcatcac gtcgagggtg atggcgtcgg
     1981 ggcggcgttc gcgcgcgcgc gcgagcccct cggcgccgtc ggcggcctcg tccacggaaa
     2041 atccctcgcg ggccaggaag cggcgcatca gcgcgcggac cggcgcctcg tcgtcgatca
     2101 cgagcacccg tcccgcaccc ccaccggtca cggcctcccc ggcggggacg ggcgccgcac
     2161 cctcggccgc cgagccgacg tccaccggca gccggacggt gaaggtggag ccgaccccgg
     2221 gggtgctctc gacgtcgatg ctgccgccca tcatctcgca gaagcggcgg gtgatggcga
     2281 ggccgaggcc ggtgccgccg aagcgccggg tggtggaagc ctccgcctgg gagaacgcct
     2341 cgaagatccg cgactgctgc tccggcgtca tcccgatccc ctggtccgtc acgcggaaca
     2401 cgacgtcgtg cccgccgccg cccgccgccg tgcgcgacac atcgagcgaa atggaccctc
     2461 gctcggtgaa cttggtggcg ttcgagagga ggttgaggag catctgccgc accttggtca
     2521 ggtcggcgcg gagcggcccg agctccgccg ccacccgcac gtcgagccgg ttgccgcggg
     2581 cgaccacgag gggctggatg gtggtgcgca cctcctcgac cagcggccgg acgtcgaagg
     2641 tctcgagata gagctccatc ttgccggcct cgaccttgga gaggtcgagc acgtcgttga
     2701 tgagcgccag gaggtgcttg cccgacgagc ggatcttctc gaggtcggcg ctccaggcgc
     2761 cgttgccctg gtcgtcggcg tcctccttga gcatctcgct gtagccgatg atggcgttga
     2821 gcggggtgcg gagctcgtgg ctcatgttgg ccaggaagcg gctcttggcc tggttggccg
     2881 attccgcctc ccgcttggcc tcgagcaggt cggtcacgtc gtggtagagc gccaggatgc
     2941 cgatgttccg cccctcgatg gccaccggca ccgccgagat ctcgacgtcg atgaggctgc
     3001 catccttgcg gcggcgcttg ccgatcccgc ggatggcgcc ctggctcgcc tgcgaggtgt
     3061 aggcgttggc ctccgagcgg gtccgctcgt cgctgatgag gccatcgagg tcgcgcccga
     3121 gcgcttcctc gcgggtgtag ccgaagaggc cctcgaaggc cgggttgagc gccgtgatcc
     3181 gcccgtcggt ctcgagcgtc acgatggcca cggggctgtt ggccacgacc gcgccgaaga
     3241 actcccgctc ccgctgggcc gcggtgtaga gccgggcgtt ctcgatcgcg atggcggcct
     3301 gtggggcaaa gagctgcagg agccggaggt cgtcggcgcc gaagtggcgg gtcgggtcgg
     3361 cgtgggtgct cgcgatggcg cccacgaggc gcgggccgat gaggagcggc gccaccatca
     3421 cggcctgcac ggtgtcgcgc acgtcggagt acttggtgga gcggccggcc cactcgtcgt
     3481 agttggggat gatcagcggc tcgtgggaga cggccacctg gcccatcgcc ccctcgccca
     3541 gggccagccg ggtgccggtc gaatc
//
LOCUS       DASUAP010000104         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_806717, whole genome shotgun sequence.
ACCESSION   DASUAP010000104 DASUAP010000000
VERSION     DASUAP010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806717"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VFU45_02580"
     CDS             complement(<1..1185)
                     /locus_tag="VFU45_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357980.1"
                     /translation="MRRPTLIAAGLTAAMVAARIFGAAPISDPTGAALPASLHLHFPI
                     LNTLLAPLFDTWDGVSMLGMRQLRAFFIWAAVAIVLWRVVRPRRWNARTLLTEAATII
                     GSVLLLLLFGLVGLAWHRPMASLAGVPAGWMVVDLHTHTNVSHDVHGTLMRGFDAEAA
                     RRWHARSGYDAFFITDHNRIDGIPAGGGPPDARPFLCPGEEVSAADQHVVLLGNTALV
                     DNRSYGDSLPGILRLFHDADPRFGAVAIASIPEYDVHHFHDLARFADAGVGGFEVVNG
                     APKANELTLAHRDSVVALARSRDLALLGVSDSHGWGATAEGWTLIRADSGQTLGRSGA
                     QAVSGFPAACAAILAALRSGYDATQIVARHRLRPDAWWPRWLTPVAVIWEGWRAVGLA
                     QAA"
     gene            1394..1636
                     /locus_tag="VFU45_02585"
     CDS             1394..1636
                     /locus_tag="VFU45_02585"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HEU5357981.1"
                     /translation="MIDFTDGDPLEDEFPLGDGTADTEAEVRCPWCGATTVISLDPGG
                     GSAQEYVEDCEVCCRPWTVHVVYDAAGNASVELEGE"
     gene            complement(1879..2589)
                     /locus_tag="VFU45_02590"
     CDS             complement(1879..2589)
                     /locus_tag="VFU45_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5357982.1"
                     /translation="MTEPLTRRDLFRMGLVAGAGALVSGCGWDGGPLKPALGAMAGLN
                     DWVGERLTSRHRLAREYAVSERSGSAFPMYYVSDELPVLADPAGWHLQVTGLVKKPLT
                     LTLAQVQALPAVTYTVKHHCVEGWTAIATWAGVPFATIAALVEPTPAARYVRFDSFDA
                     DYYNGWDLRTAMHPQTILAYAYNDRPLMPDHGAPLRLYSPLKLGYKLTKYLTTVTFTA
                     ERPGGYWEDQGYPWLGGL"
     gene            complement(2582..3265)
                     /locus_tag="VFU45_02595"
     CDS             complement(2582..3265)
                     /locus_tag="VFU45_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEU5357983.1"
                     /translation="MTSVPAPPGVRRHHWLVRLTHWVNAIVLTGLIASGLQIYGAYSR
                     FGLKGGMRIGPNPFEGRSFPHWARLGGWLAGGLNWHFALGWFFIGAGLAYVAYLAFSG
                     EWRSLIFRPGDLGPAIQMQQYYLGLRKEHPPQGKHNALQKGAYTFIVALGALSALTGV
                     SIYKPVQFHWLTALFGGYEFARYWHFVAVWVFVAFTLAHIALVFLVDPASLRAMLSGW
                     YRGRFPSHD"
BASE COUNT          592 a         1123 c         1253 g          511 t
ORIGIN      
        1 ggcggcttgc gccagtccca cggcccgcca cccctcccag atcaccgcca ccggcgtgag
       61 ccagcgcggc caccaggcgt ccggccgaag ccgatgccga gccacgatct gagtggcgtc
      121 gtagccgctc cgtagggcgg cgaggatggc cgcacaggcc gccgggaacc cggagaccgc
      181 ttgtgcgcct gagcgtccga gcgtctgtcc actgtcggcc cgaatgagcg tccacccctc
      241 cgccgtcgct ccccatccgt ggctgtcgct cacgcccagc agcgccaggt cgcgcgagcg
      301 ggccagcgcg acgaccgaat cccggtgggc caaggtgagt tcgttcgcct tcggcgcgcc
      361 attgacgact tcgaagcccc cgacgcccgc atcggcgaag cgcgcaaggt cgtggaagtg
      421 gtgcacgtca tactcgggga tcgacgcgat ggccaccgcg ccgaagcgtg gatcggcgtc
      481 gtggaagagc cggaggatcc cggggagcga atcgccatat gaccggttgt cgacgagcgc
      541 ggtgttgccg aggaggacca cgtgctggtc ggcggcgctc acttcctcgc cggggcagag
      601 gaacggtcgg gcgtcgggcg ggccgccccc cgccgggatc ccgtcgatgc ggttgtggtc
      661 ggtgatgaag aatgcgtcgt agccggaccg cgcgtgccac cggcgggcgg cctccgcgtc
      721 gaacccgcgc atcagcgtcc cgtggacgtc gtgggagacg ttggtgtggg tgtgcaggtc
      781 gaccaccatc catcccgcag ggaccccggc gagcgacgcc atcggccggt gccaggccag
      841 gcccaccagc ccgaagagga ggaggagaag gaccgacccg atgatggtcg cggcctcggt
      901 gagcagcgtg cgggcgttcc agcgccgcgg ccggaccacc cgccatagca cgatggccac
      961 cgcagcccag atgaagaacg cacgcagctg gcgcatcccc agcatcgaca cgccgtccca
     1021 ggtgtcgaag agcggcgcca ggagggtatt gaggatcggg aagtgcaggt gcagggatgc
     1081 cgggagcgcc gctcccgtcg ggtcgctgat cggcgccgct ccgaagatcc gggcggcgac
     1141 catcgcggcc gtcaggcctg cggcgatcag ggtggggcgg cgcatggtgg gaatggtaaa
     1201 cggtgaatgg tcaacggtga acggggaggt gtagtgggaa tagggaagag ggaagagtga
     1261 acgaggggag ccgggccggg aaatgcggca ctgcggctga acgctggtcg gagacggtcg
     1321 atgcaggggt cagggcaccg ctcacgacgc ccgttcaccg ttcactgttc accatttacc
     1381 cttcaacccc accatgatcg acttcaccga tggcgacccg cttgaggatg aattcccgct
     1441 gggtgacggc accgccgaca ccgaggccga ggtccgctgt ccatggtgtg gcgcgaccac
     1501 ggtcatttcg ctcgatcccg gtggcgggag tgcacaggaa tacgtcgagg actgcgaggt
     1561 ctgctgccgg ccgtggacgg tccatgtggt ctacgatgcc gccggcaacg cgagcgtgga
     1621 gctggagggg gagtgaggag ggagaagggg aaggagaagg agaaggagaa ggaaggaaaa
     1681 gaagtgaaga gtgaagagtg aaaaggggct ccggcggccc ttcgcgaccc ttgcgcgagc
     1741 tctatcaggc gcgatgctcc gcccacgtcc cacgtcccgc cattccccga attctgcgct
     1801 tcgttccttc tcacttctcc ttctcacttc tccttctcac ttctcctttt cccttttccc
     1861 ttttcccttt ttactcctct acagcccgcc gagccacgga tatccctggt cttcccagta
     1921 gccgcccggt cgctcggccg tgaacgtgac cgtggtcagg tacttggtca gcttgtaccc
     1981 gagcttgagc ggggaataga gccggagcgg cgcaccgtgg tccggcatca gcggccggtc
     2041 gttgtaggcg tacgccagga tggtctgcgg gtgcatggcc gtccgcaggt cccacccgtt
     2101 gtaataatcc gcgtcgaacg aatcgaagcg cacatagcgc gccgctggtg tcggctcgac
     2161 cagcgccgcg atcgtggcga acgggacccc cgcccacgtc gcgatggcgg tccacccttc
     2221 gacgcagtga tgcttgacgg tgtaggtcac cgccgggagc gcctgcacct gcgcgagcgt
     2281 cagggtcagg ggcttcttga cgaggccggt cacctgcagg tgccatcccg ccgggtcggc
     2341 gagcacgggg agttcgtcgc tcacgtaata catcgggaag gcgctgcccg accgttcaga
     2401 gacggcgtac tcgcgcgcca gccggtggcg ggaggtcagg cgctcgccga cccagtcgtt
     2461 gaggccggcc atggcgccga gcgcgggctt caaggggccg ccgtcccagc cgcagcccga
     2521 caccagcgcg ccggcgccgg ccaccaggcc catccggaag aggtctcggc gagtcagggg
     2581 ctcagtcatg gctggggaac ctcccgcggt accagccgct cagcatcgcc cggagggagg
     2641 ccgggtcgac caggaagacg agggcgatgt gggccagggt gaatgcgacg aagacccaga
     2701 ccgccacgaa gtgccagtag cgcgcgaatt cgtagccgcc gaatagggcg gtgagccagt
     2761 ggaactggac cggcttgtag atcgagacgc cggtgagcgc ggagagggcg ccgagcgcca
     2821 cgatgaacgt gtaggcgccc ttctggagcg cattgtgctt cccctggggc ggatgctcct
     2881 tccggaggcc caggtaatac tgctgcatct ggatggcggg gcccaagtca ccgggccgga
     2941 agatgagcga gcgccactcg ccggagaacg cgaggtacgc cacgtaggcg agcccggcgc
     3001 cgatgaagaa ccagccgagg gcgaagtgcc agttgagccc gccggcgagc cagccgccca
     3061 gccgcgccca gtgggggaag ctccggccct cgaaggggtt ggggccgatc cgcatccccc
     3121 ctttgagccc gaagcgggag tatgcgccgt agatctggag cccgctcgcg atcagcccgg
     3181 tgaggacgat cgcattgacc cagtgggtga ggcgcaccag ccaatggtgg cggcgcacgc
     3241 cgggcggggc ggggactgag gtcatcgggc ctccggtggg atgttcggct ctaacgctgc
     3301 cggcgggcga acggttccgc ctgaccgccc tacccgccct accgccggaa caggtacgcg
     3361 aacttgacga agaatccgtc gttctgccgc accaggcgcg agaacgtgag cgtgttggtc
     3421 ccgccgtcga ggctggagcc gtagccgaag aaggccacgg tgccggggct cggctggta
//
LOCUS       DASUAP010000105         3171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_806726, whole genome shotgun sequence.
ACCESSION   DASUAP010000105 DASUAP010000000
VERSION     DASUAP010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806726"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFU45_02600"
     CDS             complement(<1..398)
                     /locus_tag="VFU45_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357984.1"
                     /translation="MTAPTPHEWQHHLEDEADAAFLYRELAAAEPDAEKRRIYQELAH
                     VEDRHTEIWRKLIADAGHPGRAVRPSVRARTRAGLARRCGPGFLLPMLLEEEGREVKG
                     YLSLYKRTPAGNAQDTALLLARESAEHAESL"
     gene            645..1166
                     /locus_tag="VFU45_02605"
     CDS             645..1166
                     /locus_tag="VFU45_02605"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5357985.1"
                     /translation="MADADVALAQARTGDPAGFESLYRAFEGPVYTLARRICRTAEDA
                     EDVLQETFLEVHRSLGRYRGEGSLWGWVRTIAASKALMRLRRNKYRDAEPLEDDIHPG
                     RPGDEHLRMDLEAALERLPETSRAVVWLHDVEGYTHEEIAELMGKTASFSKSQLARAH
                     QKLRRWLGEEVAP"
     gene            1163..1768
                     /locus_tag="VFU45_02610"
     CDS             1163..1768
                     /locus_tag="VFU45_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357986.1"
                     /translation="MTHLTVEQLNDIREPGVEPGTAAAREHLESCAHCRAELDRLHQR
                     VARLRALPALRPARDRWPVVAAALDRSHRARRTRWVSAGSLALAASVALVLMAGDLFR
                     PAPATAEQQIHQAMRESSALEGELHRIHPDRRASDLTTAQVASELEQRIADLDQQLQL
                     LQLEPARPVDNARTLELWRQRVGLLDALVDVHLTHASNVGL"
     gene            1785..2819
                     /locus_tag="VFU45_02615"
     CDS             1785..2819
                     /locus_tag="VFU45_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5357987.1"
                     /translation="MLKHGLTFLMAAAVAAPVYAQQGGTVTLRRIPGDVQYRASRPGT
                     FNITVSGRGRIGVMVDMRPGPNDSIGATVQSVTPGGPAAKAGIQGGDIITKLKGTPLV
                     DRSARPSTSDDDGEAPRSQPGLKLLDLASGLSAGDTVAVEYRRGKVRKTVQLVTSDMD
                     GMMTYSVSGDTGMPFFFRPAPGGAFQYGFKLPEPGQMPFAGSFGPDQMFPAMRDQVFL
                     RIGSPLGDMEFAPINPDLGRYFGVTDGILVLSVPDSAPLALKPGDVILTIGDRKPNSV
                     PKLLGILQTYDDGETVALDVMRDHKHVTVSGKADWSQPRMIRDMPFKVERDKVKIRGD
                     QLKIRDDQRD"
     gene            2883..>3171
                     /locus_tag="VFU45_02620"
     CDS             2883..>3171
                     /locus_tag="VFU45_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357988.1"
                     /translation="MRQLVASLVLAFAFTAVSAAPVAAQSDSGLPAHYSINPVAVSRS
                     GLYFEPYRPPHPGWRYTVQLDYGNMIEYSISPDAREYILDAEAATFHLNASR"
BASE COUNT          481 a         1167 c         1064 g          459 t
ORIGIN      
        1 agcgactcgg catgctccgc cgactccctc gcgaggagga gtgccgtgtc ctgcgcgttc
       61 ccggcgggcg tgcgtttata gagcgagagg taccccttca cctcgcgccc ctcctcctcc
      121 agcagcatcg gcaggaggaa cccggggccg cagcggcggg cgagcccggc ccgtgtccgg
      181 gcccgcaccg acgggcggac cgccctgccg gggtggcccg cgtcggcgat cagcttccgc
      241 cagatctcgg tgtggcgatc ctcgacgtgc gccagttcct ggtagatccg ccgcttctcc
      301 gcatcaggct cggcggcggc gagttcgcgg tagaggaagg ccgcgtcggc ctcatcctcg
      361 aggtggtgct gccattcatg gggggtgggt gcggtcatcc ggacaatcta agcggtgcgg
      421 ggacggggca tcggcggccg ccggcgccga ccggcgaccg agtgccttcc cccaggctgt
      481 tggcacctaa gtcattgtaa aacaatgtgt tacgagcatt cctggaggtg gccctccata
      541 agtcgcgcct gtcccttcac cgtcctccgg gcgccgcgca acctgccctg caaccaaccc
      601 ccggcccccc gcatctttag ggcacgatgg cggactccta cctaatggca gacgcggacg
      661 tcgctctcgc ccaggcccgc accggcgatc ccgccggctt cgaatcgctc tatcgggcgt
      721 tcgagggccc ggtctacacc ctggcccgcc ggatctgccg gacggccgag gacgccgagg
      781 atgtgctgca ggagaccttc ctggaggtgc accgttcgct cggccggtac cggggcgagg
      841 gcagcctctg ggggtgggtc cggaccatcg cggcgagcaa ggcgttgatg cgcctgcggc
      901 gcaacaagta ccgcgacgcc gagccgctcg aggacgacat ccatcccggc cgtcccggcg
      961 acgagcacct gcggatggat ctggaggcgg cgctcgagcg cctcccggag acctcccggg
     1021 cggtggtctg gctgcacgac gtcgaaggct acacccacga agagatcgcg gaactgatgg
     1081 gcaagacggc aagcttttcg aagtcccagc tcgcccgcgc gcaccagaag ctgcgccggt
     1141 ggctgggaga ggaggtcgca ccatgacgca cctgacggtg gaacagctca atgacatccg
     1201 ggagcccggc gtcgagcccg gcactgcggc tgcgcgggag catctcgagt cctgcgcaca
     1261 ctgccgggcg gagctcgacc ggctgcacca gcgtgtggcg cgactgcggg ccctgcccgc
     1321 gctccgcccg gcgcgcgacc gctggcccgt ggtggcggcc gcgctcgacc ggagccatcg
     1381 ggcccgccgc acgcgatggg tcagcgccgg gtcgctggcg ctggcggcct cggtggcctt
     1441 ggtgctgatg gcgggcgacc tgttccgccc cgcccccgcc accgccgagc agcagatcca
     1501 ccaggccatg cgggaatcgt ccgcgctcga aggggagctg caccgcatcc accccgaccg
     1561 gcgcgcgagc gacctgacca ccgcccaggt ggccagtgag ctggagcagc gcatcgcgga
     1621 tctcgatcag cagctgcagc tgctgcagct ggaaccggcg cggccggtcg ataatgcccg
     1681 caccctcgaa ctctggcgcc agcgcgtcgg cctgctcgac gccctggtcg acgttcacct
     1741 cacgcacgcg agcaatgtgg gcctttgagg cccgggagat gccgatgttg aagcatggtc
     1801 tgacgttcct gatggccgcg gccgtcgccg cgccggtgta cgcccagcag ggcggcaccg
     1861 tcacgctccg gcggatcccc ggcgacgtcc agtaccgcgc cagccggcca gggaccttca
     1921 acatcaccgt gagcggccgg ggccggatcg gcgtgatggt ggacatgcgc ccggggccaa
     1981 acgactcgat cggcgccacg gtccagagcg tcaccccggg ggggccggcc gccaaggccg
     2041 ggatccaggg cggcgacatc atcaccaagc tcaagggtac cccgctggtc gaccgttccg
     2101 cccggccctc gacctcggac gatgacggtg aggccccccg ctcccagccc ggcctcaagc
     2161 tcctcgacct ggccagcggg ctctcggccg gcgacaccgt ggccgtggag taccgccgcg
     2221 gaaaggtgcg gaagaccgtc cagctcgtga cctcggacat ggacgggatg atgacgtact
     2281 ccgtctcggg cgacaccggg atgccgttct tcttccggcc cgcgccgggc ggggcgttcc
     2341 agtacggctt caagctcccg gagccgggcc agatgccctt cgccggcagc ttcgggccgg
     2401 accagatgtt cccggccatg cgcgaccagg tcttcctccg catcggctca cccctgggcg
     2461 acatggagtt cgcgccgatc aaccccgacc tcggccgcta cttcggtgtg accgacggga
     2521 tcctggtcct gagcgtgccg gactccgcgc ccctggcgct caagccgggc gacgtgatcc
     2581 tcaccatcgg cgaccgcaag cccaactcgg tccccaagct cctcggcatc ctccagacct
     2641 acgacgatgg cgagaccgtc gcactcgacg tgatgcgcga ccacaagcat gtcaccgtct
     2701 ccggcaaggc cgactggagc cagccccgca tgatccggga catgccgttc aaggtggagc
     2761 gggacaaggt caagatccgc ggcgatcagc tcaagatccg cgacgatcag cgcgactgat
     2821 caggtcggtc gcactggagc gagcccccgc caagacctgg cgggggctcg ttactttccg
     2881 ggatgcgcca actcgtcgcg tcgctcgtgc tcgccttcgc cttcaccgcc gtgagtgccg
     2941 cccccgtcgc cgcccagagc gacagcggcc tcccggccca ctactccatc aatcccgtcg
     3001 ccgtgagccg gagtggcctc tacttcgagc cgtaccggcc cccgcacccg ggatggcgct
     3061 acacggtcca gctcgactac ggcaacatga tcgagtacag catctcgccc gacgcccggg
     3121 agtacatcct cgacgccgag gcggccacgt tccacctgaa cgcgagccgt g
//
LOCUS       DASUAP010000106         5644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_166499, whole genome shotgun sequence.
ACCESSION   DASUAP010000106 DASUAP010000000
VERSION     DASUAP010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166499"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..998
                     /locus_tag="VFU45_02625"
     CDS             147..998
                     /locus_tag="VFU45_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEU5357989.1"
                     /translation="MTNDSLKSTVREKYGQAALRVVEGQKSGCCGGAASCGPDPITSD
                     LYSRLETEGLPSDAVLASLGCGNPTALAQLEPGDVVLDLGSGGGIDVLLSARRVGPTG
                     KAYGLDMTDEMLALARANQAKAGATNVEFLKGDIEHIPLPDASVDVIISNCVINLSAD
                     KSKVIREAFRVLKPGGRFAVSDVVMRGAVPEPVRKSMELWVGCVAGALEEQEFLALLA
                     EAGFQDPSIEPTRIYEFEDAKAFLGSAGLDSEVLAREVGGRVMGAFVRATKPAAAKCG
                     CGPGCCP"
     gene            1015..1461
                     /gene="arsN2"
                     /locus_tag="VFU45_02630"
     CDS             1015..1461
                     /gene="arsN2"
                     /locus_tag="VFU45_02630"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HEU5357990.1"
                     /translation="MELRAAGPGDTPAIEQLLASQQLPTVGNRDGSVELFVTVEGGRV
                     VGAIGLEVRGAYALLRSAVVDPGRRGQGIGRALVARVLAEARARGLRAVYLLTTTAER
                     YFPAFGFVPTDRAAAPAEMQATDEFAHACEATAVAMVLPLEPTRGP"
     gene            1461..2213
                     /locus_tag="VFU45_02635"
     CDS             1461..2213
                     /locus_tag="VFU45_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5357991.1"
                     /translation="MRYALIADIHANLPALEAVLVDLDTRKVVDATYHLGDLVGYAPW
                     PNEVVETLRKRGIAGVAGNYDSTVGSDHKHCGCQYEDPRQEELSHLSYAWTRAHVAAE
                     TRRFLAGLPFRLDLRPLGGHVAGPRLILVHGAPTLNTLYWTEGRDDDFARKMIGLVGA
                     AAGDVIAFGHTHLPWHRAVDGVHLVNAGSVGRPKDGDWRAGYVTVGFDGAGIDVEFRR
                     VEYDVARADAAIRASGLPGDFADFLRRGGAAQ"
     gene            2210..3292
                     /gene="arsB"
                     /locus_tag="VFU45_02640"
     CDS             2210..3292
                     /gene="arsB"
                     /locus_tag="VFU45_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEU5357992.1"
                     /translation="MTAAAVPAPATASVARRLSTLDRYLPLWILAAMAGGLFLGRAVP
                     TLGPALDRLQVAGVSIPIAIGLLWMMYPVLAKVRYDAIGGHIKDGRLLGTSLLLNWVI
                     GPVVMFTLAWLLLPDLPAYRNGLILVGLARCIAMVLIWNDLACGSAELAAVLVALNSL
                     FQILTYSVLGWFFLSVVPGWLGAAGTALDVSMGAIARSVLIFLGIPLAAGWATRRILR
                     RRRGDVWYDQVFLRRIGPTALLGLLYTIVLMFAMQGGRLLAHPFDVVRIALPLLIYFG
                     VMFAAGFLLARRLGFGYPATASLAFTAAGNNFELAIAVAVATFGLASGEAMAAVVGPL
                     VEVPALIALVYVALWARRFFPVEPAA"
     gene            complement(3311..4216)
                     /locus_tag="VFU45_02645"
     CDS             complement(3311..4216)
                     /locus_tag="VFU45_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5357993.1"
                     /translation="MRRILVPLDGGPFAEQALAVAVSIARTHKARLDIVAVHAPLPPM
                     APEEPLERVEAIHTAMRTELVTYAGDLAARTIKAHGINAVAAVLDGDPATELERFAAA
                     GKVDLVVMCTHGRGPVARAWLGSVADRLLRSFDGRILLLRPNGEKKFESIFRVILVAL
                     DGGALAETAIPAARALLAPGGTLVLARAVAPPVVAPLAFEMPVLPPMVTAGELRDAAE
                     KYLDGQRARLSAAGVTVRTALGESTNTAEWLVDRAKMEGAGSIAISTHGRKGLERLVL
                     GSVADKVIRSAGVPVLACHPTPPVL"
     gene            complement(4241..4972)
                     /locus_tag="VFU45_02650"
     CDS             complement(4241..4972)
                     /locus_tag="VFU45_02650"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU5357994.1"
                     /translation="MPNALGGLAPLWLWATLAIGVARTPAIRINLPAHRLELIDSGRV
                     IHSYRIAIGDTTYPTPTGRFWVRRIVWDPAWIPPVKEPWARDRKPEAPGPDNPMGPVK
                     LPLGGGYYIHGTPRSIARRRPPTHGCIRLRSPDAIELARYLEAATLAAGSADSAGIRD
                     TAGERTVRLPTVVRVDVRYDLLELRRGRIIANPDPYLRRTAASDSAGARLLAAAGFPA
                     DSALAFVSRLLDSARVGAVSAPLRR"
     gene            complement(5041..5442)
                     /locus_tag="VFU45_02655"
     CDS             complement(5041..5442)
                     /locus_tag="VFU45_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5357995.1"
                     /translation="MRVTDLMTRKVVTIARDAIVAEAVGLLADQHISAVAVVDEHERL
                     VGVLSTTDILRAESEAKDAESRTVLFDDTRVEELMSPRPLTIAPEADAREAARQLLYG
                     DVHRLFVEAQGDLVGVISDSDLIRALLVGAR"
BASE COUNT          752 a         1975 c         2036 g          881 t
ORIGIN      
        1 tcgatcgcag atatagatcg ctcggagatc acctgagcgt gacttgtacg catcaatgaa
       61 acttgttggg ccgttcaccg ttcaccgttc accgttcacc gttcaccgtt caccgttcac
      121 cgttcacctt catcgaccaa cgaccaatga caaacgactc cctcaaatcc accgtccgcg
      181 agaagtacgg ccaggcggcg ctccgcgtcg tcgaaggcca gaagagcggc tgctgcggcg
      241 gcgcggcgag ctgcggcccc gacccgatca cgagcgatct ctactcccgg ctcgagaccg
      301 aaggcctgcc ctcggacgcc gtcctggcga gcctcggctg cggcaatccg accgcgctcg
      361 cgcagctcga gcccggtgac gtggtcctcg acctcggctc cggcggcggg atcgacgtgc
      421 tgctctcggc ccggcgcgtc gggccgaccg gcaaggccta cggcctcgac atgaccgacg
      481 agatgctggc gctggcccgc gccaaccagg ccaaggcggg cgccaccaac gtcgagttcc
      541 tcaagggcga catcgagcac atcccgctcc ccgacgcctc ggtcgacgtg atcatctcca
      601 actgcgtcat caacctctcc gccgacaagt cgaaggtcat ccgcgaggca ttccgggtgc
      661 tcaagcccgg cggccgcttc gcggtctccg acgtggtgat gcgcggcgcg gtgcccgagc
      721 cggtccggaa gagcatggaa ctctgggtcg gctgcgtggc gggagcgctg gaggagcagg
      781 agttcctggc gctccttgcc gaggcgggat tccaggaccc gtcgatcgag cccacccgca
      841 tctacgaatt cgaggatgcg aaggcgttcc tcggatctgc cggcttggat tccgaggtgc
      901 tggcccgcga ggtcggcgga cgggtgatgg gggccttcgt ccgcgccacc aagcccgccg
      961 cggccaagtg cggctgtggc cccggctgct gcccctgacc gagggagata cccgatggaa
     1021 ctgcgcgcgg ccggccccgg cgacactccg gcgatcgaac aactgctggc ctcccagcag
     1081 ctcccgacag tgggcaaccg cgatggatcg gtcgaactgt tcgtgaccgt ggagggcggc
     1141 agggtggtcg gcgcgatcgg gctcgaggtg cggggggcct acgcgctcct ccgctcggcc
     1201 gtggtcgatc ctggtcgacg agggcagggg atcgggcggg cgctggtggc acgggtgctg
     1261 gccgaggcgc gggcccgggg gctccgggcg gtgtacctgc tgaccaccac cgccgagcgg
     1321 tactttccag ccttcggctt cgttccgacc gaccgcgccg cggcgccggc ggagatgcag
     1381 gccaccgacg agttcgccca cgcctgcgaa gccaccgcgg tcgccatggt gctcccgctc
     1441 gagccgaccc ggggtccctg atgcgctacg cgctgatcgc cgacatccac gccaatcttc
     1501 cggccctcga ggcggtgctc gtggacctcg atacccgcaa ggtcgtggac gccacctacc
     1561 acctgggcga cctggtcggc tacgcaccgt ggcccaacga ggtcgttgag acgctgcgga
     1621 agcgggggat cgccggcgtg gcggggaact acgactcgac cgttggcagc gaccataagc
     1681 attgcggctg ccagtacgag gatccccgcc aggaggagct ctcccacctg agctacgcct
     1741 ggacccgcgc ccacgtggcg gccgagaccc ggcgcttcct tgcgggactc ccgttccggc
     1801 tcgacctgcg gccgctgggc gggcacgtcg cggggccccg gctcatcctg gtccacggcg
     1861 cgcccacgct caacaccctc tattggaccg aagggcggga cgacgacttc gcgaggaaga
     1921 tgatcggcct cgtgggggca gcggcggggg acgtgatcgc cttcgggcat acccacctgc
     1981 cgtggcaccg cgccgtcgac ggcgtccacc tggtgaatgc cgggagcgtc ggccgcccga
     2041 aggacgggga ctggcgggcc ggctacgtga cggtcggatt cgacggcgcc ggcatcgacg
     2101 tcgaattccg ccgggtcgag tacgacgtgg cgcgggccga cgccgccatc cgcgccagcg
     2161 ggctgccggg cgactttgcc gacttcctcc ggcgcggcgg agcggcgcag tgacggccgc
     2221 tgcagttccc gcccctgcca ccgcgtcggt ggcccgccgg ctctccaccc tcgaccgcta
     2281 cctcccgctc tggatcctcg ccgcaatggc ggggggcctc ttcctcggcc gcgccgtgcc
     2341 gacactcgga ccggcactcg accggctcca ggtggcgggc gtctccatcc cgatcgccat
     2401 cggccttctc tggatgatgt atccggtgct cgccaaggtc cggtatgacg cgatcggcgg
     2461 tcacatcaag gacggcaggc tcctcggcac ctcgctcctg ctcaactggg tcatcgggcc
     2521 ggtggtgatg ttcacgctcg cctggctcct gcttccggac ctgccggcgt accgcaacgg
     2581 gctcatcctt gtcggcctgg cgcgctgcat cgcgatggtg ctcatctgga atgaccttgc
     2641 ctgcggctcc gcggagctcg cggcggtgct ggtcgcgctc aattcgctct ttcagatcct
     2701 cacgtactca gtgctcggct ggttcttcct ctcggtcgtg cccgggtggc tcggcgctgc
     2761 cggcacggcg ctcgatgtct cgatgggggc catcgcccgg agcgtcctca tcttcctcgg
     2821 catcccgctc gccgcggggt gggccacccg gcggatcctc cgccgccggc ggggtgacgt
     2881 ctggtacgac caggtcttcc tccggcgcat cgggcccacg gcgctcctcg ggctcctcta
     2941 caccatcgtc ctcatgttcg cgatgcaggg cggcaggctc ctggcgcacc cgttcgacgt
     3001 ggtgcggatc gcactgccgc tgctgatcta cttcggggtg atgttcgcag cagggttcct
     3061 gctggcgcgc cggctcggct tcggttatcc ggccactgcg tcgctcgcct tcacagcggc
     3121 ggggaacaac ttcgagctgg ccatcgccgt ggcagtggcg acgttcgggc tcgcctcggg
     3181 cgaggcgatg gccgccgtgg tggggccgct ggtcgaggtc ccggcgctca tcgcgctggt
     3241 gtacgtcgcg ctctgggccc ggcggttctt cccggtcgag cccgccgcct gacagggcgt
     3301 tgtccccgcc ttacagcacc ggcggcgtgg ggtggcaggc cagcaccggc acgccggcgg
     3361 agcggatcac cttgtcggcc acgctcccga gcaccaggcg ctccagcccc ttgcgtccgt
     3421 gggtgctgat cgcgatcgac cccgccccct ccatcttggc ccggtccacc agccactcgg
     3481 cggtgttggt cgactcaccg agcgcggtcc gcaccgtcac gccggccgcg ctgagccgcg
     3541 cccgctgccc gtccaggtac ttctcggccg catcgcggag ttcgccggcg gtgaccatcg
     3601 gcgggagcac cggcatctcg aaggcgaggg gtgcgacgac gggaggcgcc accgcgcggg
     3661 cgagcaccag cgtccccccc ggcgccagca gcgcccgggc cgcagggatc gcggtctcgg
     3721 ccagcgcgcc gccgtcgagc gccaccagga tgacccggaa gatcgactcg aacttcttct
     3781 cgccgttcgg gcgcaggagc aggatacgtc cgtcgaacga gcgcagcagc cggtccgcca
     3841 cgctgccgag ccaggcacgc gccaccgggc cgcggccgtg ggtgcacatc accaccaggt
     3901 ccaccttgcc ggcggcggcg aagcgctcga gttccgtcgc gggatcgccg tcgagcacgg
     3961 cggcgacggc gttgatgccg tgcgccttga tggtccgggc ggcaaggtcg ccggcgtagg
     4021 tcaccagctc ggtccgcatc gcggtgtgga tggcctcgac ccgctcgagc ggttcttcgg
     4081 gtgccatcgg cggaagcggg gcgtgcacgg cgacgatgtc gagccgggcc ttgtgcgtcc
     4141 gagcgatgga caccgccacc gcgagcgcct gctcggcgaa ggggccgccg tcgaggggga
     4201 ccagtatccg tctcatgggg accgtcctgt gggggtgtgt ctagcgccgg agcggcgcgg
     4261 ataccgcgcc tacccgggcg gagtcgagga gtcgggacac gaacgccagc gccgagtcgg
     4321 cgggaaaccc ggcggctgcg aggagccgcg cgccggcgct gtcggacgcc gcggtgcgcc
     4381 ggagatacgg gtcggggttc gcgatgatgc ggccgcgccg gagctccagc aggtcgtacc
     4441 ggacgtcgac ccgcacgacc gtcgggaggc gcaccgtgcg ctcgccggcg gtgtcacgga
     4501 tcccggcgct gtcggccgat ccggcggcga gggtggccgc ctcgagatag cgggcgagct
     4561 cgatcgcgtc cgggctccgg agccggatgc agccgtgggt aggcgggcgc ctgcgcgcga
     4621 tggaacgggg cgtgccgtgg atgtagtacc cgccgccgag cgggagcttg accggcccca
     4681 tcgggttgtc gggcccgggg gcctcgggct tgcggtcgcg ggcccacggt tctttgaccg
     4741 gtgggatcca cgccgggtcc cagacgattc gccggaccca gaaccggccg gtcggtgtcg
     4801 ggtaggtggt gtcgccgatc gctattctat aagagtggat gacgcggccg ctgtcgatga
     4861 gctcgagccg gtgggcgggc aggttgatcc ggatcgccgg cgtccgggcg acgccgatgg
     4921 cgagcgtggc ccagagccac aatggggcga ggccccccag cgcgtttggc acgcgtccag
     4981 tgtagggccc cgttcatgaa gctttcctga agcgtggggg agcttcgtct cagcccctgg
     5041 tcagcgggcg cccaccagga gtgcgcggat caggtcgctg tcggagatca ccccgaccag
     5101 gtcgccctgc gcctcgacga agaggcggtg cacgtcgccg tagaggagct ggcgggccgc
     5161 ctcgcgggcg tccgcttccg gcgcgatggt gagggggcgg gggctcatca gctcctcgac
     5221 gcgggtgtcg tcgaagagca ccgtgcgcga ctctgcgtcc ttggcctccg actcggcgcg
     5281 caggatgtcg gtggtggaga ggaccccgac caggcgctcg tgctcatcca ccacggccac
     5341 ggcgctgatg tgctggtcgg cgaggagacc cacggcctcg gccacgatgg cgtctcgcgc
     5401 gatcgtcacc actttgcggg tcatcaggtc ggtcacgcgc atagtcacct ccggctcagg
     5461 tcacaggtgc cgccacgtcg aaggggaata gtggccgtgc gcagatgagc cgggcgtgaa
     5521 gtggaggcga atatcgcttg cgtcgcgggt gatcagcgct cggcgtggag gcgatagccg
     5581 acgccgggtt cggtcaggag cagggaaggg cgggccgggt cgcgttcgat cttgtggcgc
     5641 agct
//
LOCUS       DASUAP010000107         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_384422, whole genome shotgun sequence.
ACCESSION   DASUAP010000107 DASUAP010000000
VERSION     DASUAP010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384422"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /gene="larE"
                     /locus_tag="VFU45_02660"
     CDS             <1..598
                     /gene="larE"
                     /locus_tag="VFU45_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEU5357996.1"
                     /translation="LLEVDTDELSDPRYLANAPDRCYFCKSELWTVLERVRERLSFDL
                     IIDGTNADDLGEHRPGLRAAAERGVRSPLAELGWTKEMVRSAARELGLPLWDAPASPC
                     LSSRIRYGLAVTPERLTQVEEGEAFLRALGVTGDLRVRHHGDRARIEVSPTQFPAIES
                     HWDGVERRFHELGFAVVELDPAGYRRGSLLAPGRDGAR"
     gene            595..1152
                     /gene="thpR"
                     /locus_tag="VFU45_02665"
     CDS             595..1152
                     /gene="thpR"
                     /locus_tag="VFU45_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEU5357997.1"
                     /translation="MIRLFAAAPVPSPALERVGGLLREFREQAWPVRWVPATGVHLTL
                     KFYGDVADERVDAIAESIAFAAAGTDPLALGLTGLGAFPTWEKARVVWLGVEAPPALE
                     LLQDRVERHAEALGFAIEGNTFRPHLTLGRVREGERLGRGAIRTLQSTSVDESFLVGE
                     VVLYQSMLGGRSPEYVPVRTFPLGG"
     gene            1152..1790
                     /locus_tag="VFU45_02670"
     CDS             1152..1790
                     /locus_tag="VFU45_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5357998.1"
                     /translation="MGCLKGTLKLLVVALLVAAVAAGWHWRHEFRAMWEFWRGGQAEA
                     APASGLATPAALAAAKDKVDSLNGWRADSVQLDAAELAALLTDGFGPILQRHLDSVHV
                     TLGTGRVEVTGRLDTGVIPRAALGPLAGLLRPREWVRVGGDVRVLGPHHAEWQVDGLR
                     IRGVRLPESMVERLVQASLGGGADGAVPVSLPAGVRALRITPTAVTIYGGGR"
     gene            1787..>2622
                     /locus_tag="VFU45_02675"
     CDS             1787..>2622
                     /locus_tag="VFU45_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5357999.1"
                     /translation="MSGERILVVDDEPGVREALKQVLEYEGYVVKTAASGGEAITVYP
                     DYRPRLVFLDVKMAGLDGLDTLTRLGALEPPAPVVMISGHGTIATAIEATRRGAFDFL
                     EKPLDSDRLLVTARNALATATLRRENAELKVAADARHAMVGTSAALEQVRGVMAKVAP
                     TPARVLITGENGSGKELVARGIHQGSPRAAGPFIEVNCAAIPSELIESELFGHIKGSF
                     TGAVADRAGKFEQADGGTIFLDEVGDMALSAQAKVLRALQEGIVTRVGGAKPIQVDVR
                     VLA"
BASE COUNT          341 a          901 c         1010 g          370 t
ORIGIN      
        1 gctcctcgag gtggacaccg acgagctctc ggatccgcgc tacctcgcca acgcccctga
       61 ccgctgctac ttctgcaaga gcgagctctg gaccgtgctg gaacgggtcc gcgagcgcct
      121 gtccttcgac ctgatcatcg acggcaccaa cgccgacgac ctcggcgagc atcgtccggg
      181 gctccgcgcc gccgccgagc gcggcgtgcg gtcgccgctc gccgagctgg gatggaccaa
      241 ggagatggtc cgctccgcgg cgcgtgagct ggggctcccg ctctgggatg cgcccgcctc
      301 gccctgcctg tcgagccgga tccggtacgg cctcgccgtc acgccggaac ggctgacgca
      361 ggtggaggag ggggaggcgt tcctccgcgc gctcggcgtc accggcgacc tccgggtccg
      421 ccaccacggc gatcgcgcgc gcatcgaggt gtccccgacg cagttcccgg ccatcgagtc
      481 gcactgggac ggcgtcgagc gccgcttcca cgagctcggg ttcgccgtgg tcgaactcga
      541 tccggcaggc taccgccggg ggagcctcct cgcacccggc cgggacggcg ctcggtgatc
      601 cggctgttcg ccgcggcgcc cgtgccttca ccggcgctcg agcgggtggg cgggctgctg
      661 cgggaattcc gggagcaggc gtggccggtg cgctgggtgc cggcgaccgg ggtgcacctg
      721 acgctcaagt tctacggcga cgtggccgac gaacgggtcg acgcgatcgc ggagtcgatc
      781 gcgttcgcgg cggccggcac cgacccgctc gcgctcggcc tcaccggact cggcgcgttc
      841 cccacctggg agaaggcgcg cgtcgtgtgg ctcggcgtgg aggcgccccc cgcgctcgaa
      901 ttgctgcagg accgggtcga gcgccacgcc gaagcgctcg gctttgccat cgaggggaac
      961 acgttccggc cgcacttgac cctgggccgg gtccgggaag gagagcgcct cggccggggc
     1021 gccatccgga ccctccagtc cacgtccgtc gacgagtcgt tcctggtcgg cgaggtggtg
     1081 ctctaccaga gcatgctggg cggccgttcg ccggagtacg tgccggtccg tacgtttccc
     1141 ctgggtggct gatgggctgc ctcaagggaa cgctcaagct gctggtggtg gccttgctcg
     1201 tcgccgccgt ggcggcgggg tggcactggc gccacgagtt ccgggcgatg tgggagttct
     1261 ggcggggcgg acaggccgag gcggcgccgg cgtccggcct cgccaccccg gctgcgctgg
     1321 cggccgccaa ggacaaggtg gacagcctca acggctggcg ggccgactcg gtgcaactgg
     1381 atgccgccga gctcgcggcg ctcctgaccg acggcttcgg gccgatcctg cagcggcacc
     1441 tcgactcggt ccacgtgacg ctcggcaccg gtcgggtgga ggtgaccggc cggctcgaca
     1501 ccggggtgat ccccagggcg gcgctcggtc cactggcggg cctgctccgg ccgcgcgaat
     1561 gggtgcgagt gggcggggac gtccgcgtgc tcggtccgca ccatgccgag tggcaagtgg
     1621 acggactccg gatccgcggc gtgcggctcc cggaatcgat ggtggaacgc ctggtccagg
     1681 ccagcctggg cggcggggcc gatggggcgg tgccggtgtc gctgccggca ggcgtacggg
     1741 cgctccggat cacgccgacc gcggtgacca tctatggagg gggcaggtga gcggcgagcg
     1801 catcctggtg gtggatgacg agcccggcgt gcgggaggcc ctgaagcagg tgctcgagta
     1861 cgagggctac gtcgtgaaga cggcggcctc cgggggcgag gcgatcaccg tctatcccga
     1921 ctaccggccc cggctcgtct tcctcgacgt caagatggcc gggctcgacg ggctcgatac
     1981 gctgacccgg ctgggcgcgc tggagccgcc ggcgccggtc gtgatgatct ccggccacgg
     2041 caccatcgcc acggccatcg aggccacgcg ccgcggcgcg ttcgatttcc tcgagaagcc
     2101 gctcgacagc gaccggctgc tggtcaccgc acgcaacgcg cttgccaccg cgacgctgcg
     2161 ccgggagaac gccgaactca aggtggccgc ggacgcccgc catgccatgg tcgggacgag
     2221 tgccgcgctg gagcaggtgc ggggggtgat ggccaaggtg gcgccgacgc cggcgcgggt
     2281 gctcatcacg ggggagaacg gcagcggcaa ggaactcgtg gcccggggga tccaccaggg
     2341 ctccccgcgc gcggccggac cgttcatcga ggtgaactgc gcggcgatcc cctcggagct
     2401 gatcgaaagc gagctcttcg ggcacatcaa ggggtcgttc acgggcgccg tcgccgaccg
     2461 ggcggggaag ttcgagcagg ccgacggcgg gacgatcttc ctcgacgagg tcggggacat
     2521 ggcgctctcc gcccaggcca aggtgctccg ggcgctccag gaagggatcg tgacgcgggt
     2581 gggtggggcc aagccgatcc aggtggacgt ccgcgtgctg gc
//
LOCUS       DASUAP010000108         4048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1106722, whole genome shotgun sequence.
ACCESSION   DASUAP010000108 DASUAP010000000
VERSION     DASUAP010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106722"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFU45_02680"
     CDS             complement(<1..407)
                     /locus_tag="VFU45_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5358000.1"
                     /translation="MTGLLVYDAVDLGGDQPFSLTVHEHETVVVTGDEASGVTDLGSY
                     ALGLAGPAGGVVLTLGTDIAGLPRRQQLAFRRKVGYLPAGDGLLQNLTLRDNIRLPLR
                     FGSNFRSDDIEGRVDVILAQLRLRPAASLRPSET"
     gene            complement(404..1189)
                     /locus_tag="VFU45_02685"
     CDS             complement(404..1189)
                     /locus_tag="VFU45_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5358001.1"
                     /translation="MTGPLARIGGATARRLSALGAPARFAFDVIGSAPSLPGAGRRVA
                     TRVLFNQLRFTALQAIPLIALLGGILSFLVIQMSIRELGQFGAADLIGKLLVVTIVRQ
                     LGPMLTAIAVVGRSGTAISAELATNSVLGEVRALEALGIDPNHYLVIPRILGCTIAVA
                     LLIVLFDVVALFGGYLAASVEGMSGGRYVANVLGALGTADVLLTVAKGLVFGAIIGLL
                     ASFHGLRVARTPTEIPRAVIRGTVSSIVLIFVMSAIFVVVFKA"
     gene            complement(1194..1913)
                     /locus_tag="VFU45_02690"
     CDS             complement(1194..1913)
                     /locus_tag="VFU45_02690"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEU5358002.1"
                     /translation="MKRVTLALLAMAIAAPVSGQAPATPQDTRPGIAVLPFDNGGSYG
                     RDKEDYAALQTGIPAVMIDELSRNPGARVVDRETIDNVLKEQDLGKNGRVDAATAAKV
                     GKIVGARYMIAGTFIELAGKFKLTARIIDVQTTEIVKSVSNDDAMMNPNDLFKIIQSV
                     AGKLMTETKLPPLPAQVAEAAKAQNVPAQALTLYSRALLYQDRGDKTKAVEYFSRAVT
                     AAGGPGKYTPADEGLKRMQQS"
     gene            complement(1929..2795)
                     /locus_tag="VFU45_02695"
     CDS             complement(1929..2795)
                     /locus_tag="VFU45_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5358003.1"
                     /translation="MKYAATFWGTRGSIPTPGPKTARFGGNTPCVAVSAGGDRLVILD
                     AGSGIRPLGRALLDAHPGAMKAEILLTHTHWDHIQGLPFFAPLNRKGNTVRIFGPRQG
                     DVALETIIDRQTDATVFPIPIKALAAELTVTEVTPGPFEVDGFRVDTLRLRHPGMTLA
                     YRLTPREGGASMAYVTDNELGPGGSYDVPPDWRRQLVRFLGDVDLLVHDGMYSDQLAE
                     ARAGWGHSTPRQAVELAAECGARHLVLFHHEPDHDDTAIDRLLEGARGAAAKAAAGLK
                     VDSAMEGLQLNL"
     gene            complement(2857..3534)
                     /locus_tag="VFU45_02700"
     CDS             complement(2857..3534)
                     /locus_tag="VFU45_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5358004.1"
                     /translation="MPLAPALVLQKVPLFHSLPEGDLTAFGELLRERVYPKGSVILFE
                     DDPGDALYIVGSGQVKVVLIGEDGREVILSVLGEGSFFGELALLDDEPRSAHVIAMED
                     STVLALRREDFQARLRSSPEVAIALLRELSSRLRRADEKIGGLVLLDVNGRVAELLLR
                     LADEEGGERITRKLTHHTVAQMIGSSRETVSRTMRDFVERGLIHVTRKDITITDRAAL
                     MQAARRS"
BASE COUNT          614 a         1366 c         1455 g          613 t
ORIGIN      
        1 gtctcggagg ggcggaggga agcggcgggg cggagccgca actgggccag gatcacgtcc
       61 acgcgtcctt cgatgtcgtc ggatcgaaag ttgctgccga agcggagtgg gagccggatg
      121 ttgtcgcgca gggtgaggtt ctgcaggagt ccgtcgcccg cgggaaggta gcccactttt
      181 cggcggaagg cgagttgctg gcggcggggc aggccggcga tgtcggtgcc gagggtcagg
      241 accacgccgc cggccgggcc ggccaggccc agggcgtagc tgcccaggtc ggtgacgccg
      301 ctcgcttcgt cgcccgtgac cacgaccgtc tcgtgctcgt ggacggtgag cgagaagggc
      361 tggtcgccgc ccaggtcgac ggcgtcgtag acgaggaggc ccgtcacgcc ttgaacacca
      421 ccacgaagat ggcgctcatg acgaagatca gcacgatcga gctgacggtg ccccggatga
      481 ccgcccgggg gatctcggtg ggcgtgcggg cgacgcgaag tccgtggaag ctggccagga
      541 gcccgatgat cgcgccgaag acgaggccct tggccacggt gaggagcacg tcggcggtcc
      601 cgagcgcgcc gagcacgttg gccacgtagc ggcccccgga catcccctcc acgctggcgg
      661 ccaggtagcc gccgaagagc gccaccacgt cgaagaggac gatgaggagc gccacggcga
      721 tggtgcaccc gaggatccgg gggatcacca gatagtggtt cgggtcgatg ccgagcgctt
      781 ccagtgcgcg gacctccccc agcaccgagt tggtggcaag ctcggcgctg atggcggtgc
      841 cgctccggcc caccaccgcg atggcggtga gcatcggccc gagctgccgg acgatggtga
      901 ccacgaggag cttgccgatg aggtcggcgg cgccgaactg gcccagctcg cggatcgaca
      961 tctggatgac gaggaagctc aggatccccc ccaggagtgc gatgagcggg atggcctgga
     1021 gggcggtgaa gcggagctgg ttgaagagca cgcgcgtcgc cacccggcgc ccggcgccgg
     1081 ggagcgaggg ggccgagccg atgacgtcga acgcgaagcg cgccggcgcc ccgagagcgg
     1141 agagccggcg cgccgtcgcg ccaccgatcc gggcgagcgg cccggtcatg ggatcacgac
     1201 tgctgcatcc gcttgagccc ttcgtcggcc ggggtgtact tcccggggcc gccggcggcg
     1261 gtcacggcgc ggctgaagta ttccaccgcc ttggtcttgt cgccccggtc ctggtacagg
     1321 agggcgcggc tgtagagcgt cagcgcctgg gccgggacgt tctgggcctt agcggcctcc
     1381 gccacctggg cggggagcgg cgggagcttc gtctcggtca tcagcttccc ggccaccgac
     1441 tggatgatct tgaagaggtc attcgggttc atcatcgcgt cgtcgttgga gaccgacttg
     1501 acgatctcgg tcgtctgcac gtcgatgatg cgggccgtca gcttgaactt gcccgccagc
     1561 tcgatgaagg tcccggcgat catgtaccgg gcgccgacga tcttgccgac cttggcggcc
     1621 gtcgcggcgt ccacccggcc gttcttcccg aggtcctgct ccttgagcac gttgtcgatc
     1681 gtctcacggt cgaccacgcg ggcgccgggg ttgcgggaga gctcgtcgat catcaccgcc
     1741 gggatgccgg tctggagcgc ggcgtagtcc tccttgtcgc gcccgtagga gccgccgttg
     1801 tcgaacggca gcaccgcgat gccggggcgg gtgtcctgcg gcgtggcggg ggcctgtccg
     1861 gagacgggcg ccgcgatcgc catcgccagc agggccaggg tgacgcgctt catgggtgtc
     1921 gctcctcgtc agaggttgag ttgcaggcct tccatggccg agtcgacctt gaggccggcg
     1981 gccgccttgg ccgccgcgcc ccgcgccccc tcaaggaggc ggtcgatggc cgtatcgtcg
     2041 tggtccggct cgtggtggaa gagcaccagg tgccgggccc cgcattccgc cgccagctcg
     2101 acggcctggc ggggggtcga atgtccccag ccggcccggg cctcggccag ctggtcgctg
     2161 tacatcccgt cgtgcaccag gaggtccacg tcgcccagga accggaccag ctggcggcgc
     2221 caatcgggcg gcacatcata agatccaccc ggacccagct cgttgtctgt gacgtaggcc
     2281 atactggcgc ccccttcgcg gggcgtcaac cggtacgcca gggtcatgcc cgggtggcgg
     2341 agccggaggg tgtccacccg gaaaccgtcg acctcgaagg ggccgggtgt gacctccgtc
     2401 accgtcaatt cggccgccaa cgccttgatc gggatgggga agacggtggc atcggtctgc
     2461 cggtcgatga tcgtctccag cgccacgtcg ccctggcggg gaccgaagat ccgtacggtg
     2521 ttgcccttcc ggttcaaggg cgcgaagaac ggcagcccct ggatgtggtc ccagtgggtg
     2581 tgcgtgagca gtatctcggc cttcatggcg cccggatgcg catcgagcag ggcgcgcccg
     2641 agggggcgga tgccgctccc cgcatcgagg atgaccaggc ggtcgccgcc ggccgagacg
     2701 gcgacgcacg gcgtattccc gccgaagcgc gccgtcttcg ggcccggtgt cgggatggac
     2761 ccgcgcgtcc cccagaacgt ggcggcgtat ttcacggaag tcggctcggc atccgaacaa
     2821 tctcgcccat tcgggccggg cctcccgtga ccggcatcac gacctccggg cggcctgcat
     2881 caaggcggcc cggtcggtga tggtgatgtc cttccgggtc acgtggatga gcccgcgctc
     2941 gacgaagtcg cgcatcgtgc gcgagaccgt ctcgcggctg gagccgatca tctgcgccac
     3001 ggtgtggtgc gtgagctttc gggtgatccg ctcgccgccc tcctcgtccg ccaggcgcag
     3061 cagcaactcg gccacccgcc cgttcacgtc cagcaggacg aggccgccga tcttctcgtc
     3121 cgcgcggcgg agccggctgg agagctcccg caggagcgcg atggccacct cgggcgacga
     3181 ccggagccgg gcctggaaat cctcccgccg gagcgcgagg accgtcgagt cctccatcgc
     3241 gatcacgtgc gcggagcggg gctcgtcatc gagcagcgcg agctcgccga agaagctccc
     3301 ctcgcccaac accgagagga tcacctcacg cccgtcttcg ccgatgagga ccaccttcac
     3361 ctgtcccgag ccgacgatgt agagcgcgtc ccccgggtcg tcctcgaaca ggatcacgct
     3421 gcccttaggg tagacgcgct cccggaggag ttcgccgaac gcggtgaggt ctccctcggg
     3481 gagggagtgg aacaggggga ccttctgcag caccagggca ggcgcgaggg gcaaccgggg
     3541 cctcaaggga tggaagtaac ggccagtagg ttacttgcac cgtttcggcg tgtcatacgt
     3601 ctgggtcgcg agtcgcgagt cgcgggtcgc gggtcgcgga tcgcgggtca cgggtcacgg
     3661 gtcgcgggtc gcgggtcgca gtaccttcgg gcggatagcg gatagcggat agcggatagc
     3721 ggatagcgga tagcggatag cggctagcgg atagccagca cccatcccac gccccaactt
     3781 gcaactaatc ccccctcccg cccgtattcc ccccgtcacc ttcgatcatc tgttctcgcc
     3841 ggtacgccgg caaccccaaa ccccgattga gcttatgaat cgatccctgg tcctcaccgc
     3901 actgtgcctg atgccgggcg cgctcctcgc ccagcggccc gaagccccgg cccagccggt
     3961 cacgatcgtc ccccagcccg agaccaagct tcccctgaag cacgcgcccg agaagaccgg
     4021 gcccgccatc accgccaagg acctgatg
//
LOCUS       DASUAP010000109         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1038514, whole genome shotgun sequence.
ACCESSION   DASUAP010000109 DASUAP010000000
VERSION     DASUAP010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038514"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..865)
                     /locus_tag="VFU45_02705"
     CDS             complement(143..865)
                     /locus_tag="VFU45_02705"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU5358005.1"
                     /translation="MTAHDWFLEHRAAWVARALDAEEARSFRDHLKRCPDCRAAVAEL
                     EGELAWLPMGVAPVAPRPGFRREALDRALGRKARRGWILPLALAASLLVAAGLVFQLE
                     RRIGALEDDVAAERTALAALRDTMSVARQAGRMLHASFAMDGRTCGMMILADEHTHRW
                     SVVVHGLPPARAGEKYQFWFITDGGMKQGTALEVVPGKVATFTTGMPPGNAKVMGAAL
                     SVEPMDNGGPEMKGPELAKIML"
     gene            complement(862..1485)
                     /locus_tag="VFU45_02710"
     CDS             complement(862..1485)
                     /locus_tag="VFU45_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5358006.1"
                     /translation="MTPARDPRSTPVLAALSAAAPASLRERLLARDEGALAELVDLAS
                     PWLLGVTQSILRDREESEEVVFEVFGTAWDRIGEVEPDPRGLMPWLLRVARNRAIDRV
                     RRRGRRHRTAEHAHALGALGGTTTAAAEINEAATPGWHVHERVHAALGQLPEDQRAAV
                     RLAYFHGLTHSEIAEELSIPMGTVKTRLRLAFDKLRQALAPLKEWVQ"
     gene            complement(1482..>2000)
                     /locus_tag="VFU45_02715"
     CDS             complement(1482..>2000)
                     /locus_tag="VFU45_02715"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEU5358007.1"
                     /translation="APLAAQAGAGMGMDKGKMDHDKMMMKDHVMPAVWFKGVAPHTAS
                     GSFTMSVKDGKHTLEISKDFKSTNSPDGFVYLAKDGKVDASALELGELSGGQGGGTFA
                     IPADAKVWEFNTVVVWSKAHNAAVAVAPLHGKMDHDSMMKAHGKMMDHDSMMKAHHDE
                     MMKHDTGMQQKP"
BASE COUNT          273 a          735 c          664 g          328 t
ORIGIN      
        1 ggaaaccgag taaagactgc gtgctgttta cgcgccgatg cggcctttca tgtggctgtt
       61 gacccgccta tgcggctgtt catgcggtcc ttcatgcggc agttcacgcg cctgttcgtg
      121 cgacctctca cgcgccaccc acctacaaca tgatcttcgc cagctccggc cccttcatct
      181 cggggccgcc gttgtccatc ggctccacgc tgagcgcggc gcccatcacc ttggcattcc
      241 ccggcggcat cccggtggtg aaggtggcca ccttccccgg caccacctcg agcgcggtgc
      301 cctgcttcat cccgccgtcg gtgatgaacc agaactgata cttctccccc gccctggccg
      361 gcggaaggcc gtgcaccacc accgaccagc ggtgggtgtg ctcatcggcc aggatcatca
      421 ttccgcaggt cctgccgtcc atcgcaaagc tggcgtggag catccggccg gcctggcggg
      481 ctaccgacat cgtgtcccgg agcgccgcga gcgccgtccg ctcggctgcc acgtcgtcct
      541 cgagcgcgcc gatccggcgc tccagctgga agacgagccc cgccgccacc aggagcgacg
      601 ccgccagcgc gagcggcagg atccacccgc gccgcgcctt ccgcccgagc gcacggtcga
      661 gcgcctcgcg ccggaacccg ggacgcggcg ccaccggggc cacgcccatc gggagccacg
      721 ccagctcccc ctccagctcg gccacggcgg cgcggcagtc ggggcaccgc ttcaggtggt
      781 cccggaagct ccgcgcctcc tcggcgtcga gcgcccgcgc cacccaggcg gcgcgatgtt
      841 cgaggaacca gtcgtgtgcg gtcattgcac ccactccttg agcggcgcca gcgcctgccg
      901 gagcttgtcg aaggccagcc gcaggcgggt cttcaccgtg cccatcggga tcgaaagctc
      961 ctcggcgatc tcggaatggg tgaggccgtg gaagtaggcc aggcgcaccg ccgcgcgctg
     1021 gtcctccggc agctgaccca gggccgcgtg cacccgctcg tgcacatgcc accccggcgt
     1081 cgccgcctcg ttgatctcgg cggctgcggt cgtcgtccca ccgagggcgc cgagggcgtg
     1141 ggcgtgttcg gccgtgcggt ggcggcgccc gcggcggcgg acgcggtcga tggcgcggtt
     1201 ccgcgccaca cggagaagcc agggcatcag gccgcggggg tcgggctcga cctcgccgat
     1261 ccggtcccac gcggtgccga agacctcgaa gacgacctct tccgactcct cgcgatcgcg
     1321 gaggatcgac tgggtgacgc cgaggagcca cggcgacgcc agatcgacaa gctccgcgag
     1381 cgccccctca tcccgcgcga ggaggcgctc ccggagggag gccggtgcgg cggcggacag
     1441 cgccgccagc accggtgtgg agcgtggatc tcgtgccggg gtcacggctt ctgctgcatc
     1501 cccgtgtcgt gcttcatcat ctcgtcgtgg tgcgctttca tcatcgaatc gtggtccatc
     1561 atcttgccgt gcgccttcat catggagtcg tggtccatct tgccgtggag cggggccacg
     1621 gcgaccgccg cgttgtgcgc tttggaccag acgaccacgg tgttgaattc ccagaccttg
     1681 gcgtcggccg ggatggcgaa ggtcccgccc ccctggccgc cggagagctc gcccagttcg
     1741 agggcggacg cgtcgacctt gccgtccttg gccaggtaga cgaagccgtc gggagagttg
     1801 gtggacttga agtccttgga gatctcgagg gtgtgcttcc cgtccttgac cgacatcgtg
     1861 aacgaacccg atgcggtgtg gggtgccacc cccttgaacc agaccgccgg catcacgtga
     1921 tccttcatca tcatcttgtc gtggtccatc ttgcccttgt ccatccccat cccggcgccc
     1981 gcctgggcgg caagcggcgc
//
LOCUS       DASUAP010000110         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_875015, whole genome shotgun sequence.
ACCESSION   DASUAP010000110 DASUAP010000000
VERSION     DASUAP010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875015"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFU45_02720"
     CDS             <1..883
                     /locus_tag="VFU45_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5358008.1"
                     /translation="FQLDPRPFQASLDQARAVAARDGAQLASAEADEKRYADLAARDY
                     VTPQQLEQARATASALRATLAADSAAVENARLSLQYTTIRAPITGRAGAILVREGNLA
                     KAQGTPLVVINQMAPILVRFAVPATALPAIRTHAAADARVHVDPTGVGGVGSDGTLSF
                     VDNAVDSSTGTILLKGRFANARQELWPGELVNVRLQLFTEKQVVTAPAAAIVSGQQGD
                     YVFVIGAGQAAATRPVKIERITGDLAVLSGVDPGETVVTDGQLKLRPGAVVQIKPGPA
                     TPAATGPTADSAAGAIR"
     gene            916..>3548
                     /locus_tag="VFU45_02725"
     CDS             916..>3548
                     /locus_tag="VFU45_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5358009.1"
                     /translation="MTTLVMSGILIFGVVAYRQLSVSDLPSVDYPTITVNAGLSGASP
                     ETMAASVATPLEKQFATIAGLDAMSSTSTQGGTSISLQFALTRNIDAAAQDVNAAISQ
                     TLRQLPQGMLPPSYQKVDPSSSPILYFALTTRTLPLSQLDEFAETFLSQRLSTVDGVA
                     AVQVYGSQKYAVRIQLDPRALAARKVGIDQVVNAVSTGNVNLPTGILWGTDKAYAVEA
                     NGQLQDAAAFRPLIVTYRDGRPVRLQELGNVLDDVQDSHVASWYDSTRAIVLAIQRQP
                     GTNTVAVAGRVRDMMRRLEAELPASVEVHTLFDRSDGIEASVRDVKFTLGLTIALVVL
                     VIFSFLRNIPATIIPSLALPMALIGTFAVMYLLGFSLDNLSLMALTLSVGFVVDDAIV
                     VLENIVRHMEMGEGVLEASLNGSREIGFTVISMTLSLVAVFLPVLLLGGLIGRLFREF
                     AITIGVAILVSGFVSLTLTPMLCSRFLKAHPEGETHGRAYQAAERMFNAALAWYRRTL
                     GWVMDRRPMAMAFSAFILLATVGLFVISPKGFLPSEDQGQLFATTETAEGTSFDAMWT
                     HQREAAAIVAQDPNVQDFMSAIGGGRGTTNQGRMFMHLKPRNERALSADEVARELTGK
                     LAAIPGLRVYIQNPPPISIGAKFSKSLYQFTLTGSDINALYSSATDLEGKMRALPGLV
                     DVTSDLQIKNPQVSVTIDRDRAAYYGIDASAVENALYDAYGARQVSTIYTPTNQYWVV
                     LELLPQYQRDLSALNMLYVTSRNGEPVPLASLATVTPSVGPLSVGHAGQLPAVTISFN
                     LAPGVALGNAVAEVQKLADATLPASIGTSFSGTAQAFKDAQSGLLGLLVLAILVIYLV
                     LGILYDSFIHPLTILSGLPFAG"
BASE COUNT          530 a         1255 c         1237 g          526 t
ORIGIN      
        1 cttccagctc gatccccgac ccttccaggc ctcgctcgac caggcccggg cggtggcggc
       61 gcgggacggc gcgcagctcg cgagcgcgga ggcggatgag aagcgctacg ccgacctcgc
      121 ggcgcgggac tacgtgacac cgcagcagct ggagcaggcg cgggccaccg cgagcgcgct
      181 ccgggcgacg ctcgccgcgg attccgccgc ggtcgagaac gcacggttga gcctccagta
      241 caccaccatc cgtgcaccga tcaccggacg ggccggcgcc atcctggtgc gggaggggaa
      301 cctcgcaaag gcgcagggca cgccgctcgt ggtgatcaac cagatggcgc cgatcctggt
      361 gcggttcgcg gtgccggcca cggcacttcc ggccatccgg acgcacgccg cggcggacgc
      421 cagggtgcac gtcgacccga ccggcgtcgg cggggtcggg agcgacggga cgctctcgtt
      481 cgtggataac gcggtcgact cgagcaccgg gacgatcctc ctcaagggac gcttcgccaa
      541 cgcgcgccag gagctctggc ccggcgagct ggtgaacgtg cggctccagc tcttcaccga
      601 gaagcaggtg gtgacggcgc cggccgccgc gatcgtctcc ggccagcagg gggactacgt
      661 gttcgtgatc ggtgcgggcc aggccgcggc gacccggccg gtcaagatcg agcggatcac
      721 cggcgacctg gcggtgctgt cgggggtcga cccgggcgag accgtggtga ccgacggcca
      781 gctcaagctc cggccggggg cggtggtgca gatcaagccg ggaccggcga cgccggcggc
      841 caccggcccg accgccgaca gcgccgcggg ggccatccga tgaacgtcgc ggagcccttc
      901 atccgccgtc cggtcatgac cacgctggtc atgtccggca tcctcatctt cggcgtggtg
      961 gcgtaccggc agctttcggt gagcgacctg ccgtcggtgg actaccccac gatcacggtc
     1021 aatgccgggc tctccggcgc cagccccgag acgatggcgg catcggtggc gacgccgctc
     1081 gagaagcagt tcgcgaccat cgcggggctc gacgcgatga gctcgacgag cacgcagggc
     1141 gggacgtcga tctcgctgca gttcgcgctg acgcgcaaca tcgacgcggc ggcgcaggac
     1201 gtgaacgcgg ccatctccca gacgctgcgc cagctgccgc aggggatgct cccgccgtcg
     1261 taccagaagg tggacccgtc gtcctcgccg atcctctact tcgcgctcac cacgcgcacc
     1321 cttccgctct cgcagctgga cgagttcgcc gagaccttcc tgtcgcagcg gctttcgacg
     1381 gtggacggcg tggcggcggt gcaggtgtac ggttcgcaga agtacgccgt gcggatccag
     1441 ctcgacccgc gggcgctggc ggcccggaag gtgggcatcg accaggtggt caacgccgtc
     1501 agcaccggca acgtcaacct gccgacgggg atcctctggg gcaccgacaa ggcgtacgcg
     1561 gtcgaggcca acggccagct gcaggacgcc gcggccttcc ggccgctcat cgtgacctac
     1621 cgcgacggcc ggccggtgcg gctgcaggag ctcggcaacg tcctcgacga tgtgcaggac
     1681 tcccacgtgg ccagctggta cgacagcacc cgggccatcg tgctcgcgat ccagcggcag
     1741 cccggcacca acaccgtggc cgtggcggga cgggtccgcg acatgatgcg gcggctcgag
     1801 gccgagctcc ccgcctcggt cgaggtgcac acgctcttcg accggtcgga cgggatcgag
     1861 gcctcggtcc gggacgtcaa gttcaccctg gggctcacga tcgcgctcgt ggtgctggtg
     1921 atcttcagct tcctccggaa catcccggcg acgatcatcc cgagcctggc gctcccgatg
     1981 gcgctgatcg gcaccttcgc ggtgatgtac ctgctcggct tctcgctcga caacctgtcc
     2041 ctgatggcgc tcacgctctc ggtcgggttc gtggtggacg acgccatcgt ggtgctggag
     2101 aacatcgtcc ggcacatgga gatgggcgag ggggtgctgg aggcgtcgct caatggctcg
     2161 cgggagatcg ggttcaccgt catctcgatg acgctctcgc tggtggcggt gttcctgccg
     2221 gtgctcctgc tgggcggcct catcggccgg ctcttccgcg agttcgccat cacgatcggc
     2281 gtggcgatcc tggtgtccgg gttcgtctcg ctcacgctga cgccgatgct ctgcagccgc
     2341 ttcctcaagg cccaccccga gggggagacg cacggccgcg cctaccaggc cgccgagcgg
     2401 atgttcaatg cggcgctcgc gtggtaccgg cgcacgctcg ggtgggtgat ggaccgccgc
     2461 ccgatggcga tggcgttctc ggccttcatc ctgctcgcca cggtggggct gttcgtcatc
     2521 tcgcccaagg ggttcctgcc gagcgaggac caggggcagc tcttcgccac caccgagacc
     2581 gccgaaggga cctcgttcga cgcgatgtgg acccaccagc gggaggcggc cgccatcgtg
     2641 gcgcaggacc cgaacgtgca ggacttcatg tcggccatcg ggggcggacg gggcacgacc
     2701 aaccagggcc ggatgttcat gcacctgaag ccccggaacg aacgcgccct ctcggccgac
     2761 gaggtggcgc gggagctgac gggcaagctg gcggccatcc ccgggctccg ggtgtacatc
     2821 cagaacccgc cgccgatcag catcggggcc aagttctcca agagcctcta ccagttcacg
     2881 ctgaccggct cggacatcaa cgcgctctac agctccgcca ccgacctcga ggggaagatg
     2941 cgcgcgctcc cggggctggt ggacgtgacc agcgacctgc agatcaagaa cccgcaggtg
     3001 tcggtgacca tcgaccgcga ccgcgccgcc tactacggca tcgacgccag cgcggtggag
     3061 aacgccctct acgacgccta cggcgcgcgg caggtgtcca cgatctacac gccgaccaac
     3121 cagtactggg tggtgctcga gctgctgccg cagtaccagc gcgacctctc cgcgctcaac
     3181 atgctctatg tcaccagccg gaacggggaa ccggtgccgc tcgcgagcct ggccacggtg
     3241 acgccgtcgg tggggcccct cagcgtgggg cacgccggcc agctgccggc ggtgacgatc
     3301 tcattcaacc tggcgcccgg cgtggccctg ggcaacgcgg tggccgaggt gcagaagctc
     3361 gccgacgcga cgctgccggc gagcatcggg acgagcttct cgggcaccgc gcaggccttc
     3421 aaggacgccc agagcgggct cctcggcctc ctcgtgctcg cgatcctcgt catctacctg
     3481 gtgctcggca tcctctacga tagcttcatc cacccgctca cgatcctgtc ggggctgccg
     3541 ttcgccgg
//
LOCUS       DASUAP010000111         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_193412, whole genome shotgun sequence.
ACCESSION   DASUAP010000111 DASUAP010000000
VERSION     DASUAP010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193412"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFU45_02730"
     CDS             complement(<1..202)
                     /locus_tag="VFU45_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358010.1"
                     /translation="MRLTPRILDRYILTSWSRIFLVTAIGLPLTTVIIELTDNINGWL
                     QDGLGWKRIALATLLRLPENMFE"
     gene            complement(199..1788)
                     /locus_tag="VFU45_02735"
     CDS             complement(199..1788)
                     /locus_tag="VFU45_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEU5358011.1"
                     /translation="MRLLDRYIIRQLVAPFLFALSGLTGLLLLNQVAAQIPKLVGKGL
                     PWSIIGEFFLLTVPFILVMTVPMSVLLATLYAFTHLAADNEITAMRASGVSVYQMLRP
                     VVLVGVALSVANFVLTDQVHPRTNARLKALIDDIGRKKPTLDLREQAINAIPPSSYFL
                     RASRIDAASGKMRDVSLFDLNDRNVRRVIYADSGRLAYEEGRNDLELRLYDGVIHEYE
                     VDHPELFQSTDYRINTIKVRNVANSLDRQADTSIMRGDREMTTCEMDSVVQGAERTAT
                     AVRADADRLRRGDLRALLRLPSLPPPQPMAAPPPRPCGLRRWLGILLPRTAEAQVPAH
                     QRPVRRLSPQGAAIRRRKLLEADSARARLAESADSARRIQAARDSVTAVRQSAPAVTL
                     VGRFRLSNTRSQLQSAIAKQNDFLVEIWKKWALSFACLIFALVGVPLALRFPRGGMGL
                     VIGAGLGLFSLYYSALIGGEALGKRGLISPFWSMWAPNVALLLGAIVGIRRVSRESGS
                     TRGGDLRDLLDSLFGRFRRAR"
     gene            1880..>2428
                     /locus_tag="VFU45_02740"
     CDS             1880..>2428
                     /locus_tag="VFU45_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358012.1"
                     /translation="MARSVALFLLLAVPIRLAAQVPAQVPADTAGPHLVAGFPPFRYT
                     IVPPEAHRLAAVLLHPDAAARTDRWSDSLRAALDSAAQDRAAAFAARALFKNSAAVAA
                     LADTGGFLGVDRRYADLTLDGQARVEVRTERDKNERCTSYQLLDPSSGCRGIFTPPSI
                     EDELTMRAAGTIGSRLHVDLDYD"
BASE COUNT          379 a          790 c          897 g          362 t
ORIGIN      
        1 cctcgaacat gttctccggg agccggagga gcgtggccag ggcgatgcgc ttccacccca
       61 ggccgtcctg gagccagcca ttgatgttgt cggtgagctc gatgatgacg gtggtgagcg
      121 gcaggccgat ggccgtgacc aggaagatcc gggaccacga ggtcaggatg tagcggtcga
      181 ggatcctggg ggtgagcctc aacgggccct ccggaagcgg ccgaagaggg agtcgaggag
      241 gtcgcggagg tcgccgccgc gggtggagcc cgactcgcgg ctcacgcgcc ggatgccgac
      301 gatggcgccg aggaggagcg ccacgttggg ggcccacatc gaccagaagg gggagatgag
      361 cccccgcttg ccgagggctt cgccgccgat cagggcgctg tagtagaggg agaagagccc
      421 gaggccggcg ccgatgacca ggcccatccc gccgcggggg aagcggagcg ccagcggcac
      481 gcccaccagg gcaaagatga ggcaggcaaa gctcagcgcc cacttcttcc agatctcgac
      541 caggaagtcg ttctgcttgg cgatcgccga ttggagctgg ctgcgcgtgt tgctcaggcg
      601 gaaccggccc accagcgtga cggccggtgc agattgacgg accgccgtca cgctgtcccg
      661 ggccgcctgg atccgccgcg cggaatcggc cgactcggcc aacctggctc gcgcagaatc
      721 ggcctccagc agctttcggc ggcggatggc cgcgccctgc ggcgacaacc tgcgcacggg
      781 ccgctggtgt gcagggacct gggcctcggc cgtcctggga aggaggatgc ccagccaccg
      841 tcgcaggccg caggggcggg gtgggggcgc ggccatgggt tgcggtgggg ggagcgaggg
      901 gagccgcagg agcgcgcgga ggtcgccgcg gcggaggcgg tcggcatcgg cccggacggc
      961 ggtggcggtg cgctccgcac cctggaccac gctgtccatc tcgcaggtcg tcatctcccg
     1021 gtcgccgcgc atgatcgagg tatccgcctg gcggtcgagc gaattggcga cgttccgcac
     1081 cttgatggtg ttgatccggt agtcggtcga ctggaagagt tcggggtgat ccacttcgta
     1141 ctcgtggatg accccgtcgt agagccggag ctcgaggtcg ttgcggccct cttcgtacgc
     1201 caggcgcccg ctgtcggcgt agatgacccg gcggacgttg cggtcgttga ggtcgaagag
     1261 cgacacgtcg cgcatcttgc cggaggcggc gtcgatccgg ctggcgcgca ggaagtacga
     1321 cgacgggggg atggcattga tcgcttgctc ccggaggtcg agcgtcggct tcttccgccc
     1381 gatatcgtcg atcagcgcct tgaggcgggc gttagtgcgg gggtggacct ggtcggtgag
     1441 gacgaagttg gccaccgaga gcgctacccc gaccagcacg accgggcgga gcatctggta
     1501 gacgctgaca ccgctcgccc gcatggcggt gatctcgttg tcggccgcca ggtgggtaaa
     1561 ggcatagagg gtggccagga gcaccgacat cggcaccgtc atcaccagga tgaacgggac
     1621 cgtgaggagg aagaactccc cgatgatgct ccatgggagc cccttgccca cgagcttcgg
     1681 gatctgggcc gccacctggt tgagcaggag cagccccgtg agccccgaca gggcgaacag
     1741 gaatggcgcc acgagctggc ggatgatgta gcggtcgagg aggcgcaagg gctctggcgt
     1801 gccggttgtt ggagtgtcta agttccagta tattaccaac ttaccgcccc tacaaggccg
     1861 catccccgac cacatcgccg tggcgcgctc cgtcgcccta ttcctcctcc ttgcggtccc
     1921 gatccggctg gcggcgcagg tacctgcgca ggtgcctgcc gatacggcgg gaccgcatct
     1981 ggtggcgggg ttccccccct tccgatacac catcgtcccg ccggaggccc accgcctggc
     2041 ggcggtcctg ctgcaccccg atgcggccgc gcgaaccgac cgctggtccg attccctccg
     2101 ggccgccctc gattccgcgg cgcaggatcg ggcggcggcc tttgcggcgc gggcgctctt
     2161 caagaacagc gccgccgtcg ctgcgctggc agacacgggc gggttcctcg gcgtcgatcg
     2221 gcggtacgcc gacctgaccc tcgacggcca ggcccgggtc gaggtccgga ccgagcgcga
     2281 caagaacgag cgctgcacgt cctaccagct cctcgatccg tcgagcggct gccgggggat
     2341 cttcacgccg ccgagcatcg aggacgagct gacgatgcgg gccgccggga ccatcggcag
     2401 ccggctccac gtcgacctgg actacgac
//
LOCUS       DASUAP010000112         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_970307, whole genome shotgun sequence.
ACCESSION   DASUAP010000112 DASUAP010000000
VERSION     DASUAP010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970307"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFU45_02745"
     CDS             complement(<1..875)
                     /locus_tag="VFU45_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HEU5358013.1"
                     /translation="MRKTLLFALGAALLLPMTAQAQAYDTNDPVLKRIWSIGMDSSWT
                     EPLSHALFDSLGPRLTGSPDQRRANDWLVRMYKSWGIDARNEQYGTWRGWRRGYSHID
                     LVAPRVRSLEGTMLAWSPGTHKKDLTASTIILPHFKDSTEFVAWLPQARGKLVLVAPE
                     KPSCRTADDWKENATPESLARMGKLWNDIRAEWADRIKATGYSLALGTGSLGVRMEKA
                     GVAGFLTSRPKDGWGTIEIFETYDTKAPAIALSCEDYGLIYRLTADNDHPKVRLNLDA
                     DLLGEQPVFNTIATIP"
     gene            complement(1017..1730)
                     /locus_tag="VFU45_02750"
     CDS             complement(1017..1730)
                     /locus_tag="VFU45_02750"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU5358014.1"
                     /translation="MNIRAFAGAIALASLTFAATPAAHAQANTLTAKEKAHGWRLLFD
                     GTSLDGWRGYKTDAAPVGWRVADGLIVKDSSAEDIVTRDSFANFELVMDWKIAKGGNA
                     GIFYRGTEEYDHIYWSAPEYQLLDDANAPDGASRLTSAGADYALYPSPAGIERPAGEW
                     NTARIVVRGDHVEHWMNGKKLLSYTLGSADWAAKVKASKFGKWPNYGKATRGLIGVQG
                     DHSGRLELRNIKIRVLPAK"
     gene            2044..2292
                     /locus_tag="VFU45_02755"
     CDS             2044..2292
                     /locus_tag="VFU45_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358015.1"
                     /translation="MSAKVSLRIALVISIAGAAFSGVLTWRELVSRVAGGCSAVGAPG
                     TIFGYPPCVYGLVMYLLLMVVTALGLRSSEGGTRPPAG"
     gene            2328..>3186
                     /locus_tag="VFU45_02760"
     CDS             2328..>3186
                     /locus_tag="VFU45_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5358016.1"
                     /translation="MTRRATLLVVLALVPARGLLAQRGGAPYRDPALPVEARVQDLLR
                     RMTPEEKFWQLYMSPVFGLPGDTATDYSHGAFGLQVFGVTTPRTTDAARAYAERVDAF
                     QRYLAEHSRLGIPIIPFDEAVHGLMEAGATDFPAAISLAATWDTALVTRVAGAIAVEA
                     RSRGVRQVLSPVINVATDVRWGRVEETYGEDPLLVSRMGAAYVGAFERAGVVATPKHF
                     VANVGAGGRDSYPIEVSDRELDRVYFPPFEAAVAAGARSVMTAYNSVDGEPASQNRRL
                     LTEVLKGAWG"
BASE COUNT          442 a         1062 c         1160 g          522 t
ORIGIN      
        1 gggatggtgg cgatggtgtt gaacaccggc tgctcgccga ggaggtcggc gtcgaggttg
       61 aggcggacct tcggatggtc gttgtcggcg gtcaggcggt agatgaggcc gtagtcctcg
      121 cacgagaggg cgatggccgg cgccttggtg tcataggtct cgaagatctc gatggtgccc
      181 cagccgtcct tcgggcggga ggtcaggaaa cctgccacgc cggccttctc catccgcacg
      241 ccgagcgagc cggtgccgag tgccagggag tagccggtgg ccttgatccg atcggcccat
      301 tcggcccgga tgtcgttcca gagcttgccc atccgcgcca gggactcggg cgtcgcgttc
      361 tccttccagt catccgccgt gcggcagctc ggcttttccg gcgccaccag cacgagcttg
      421 ccccgggcct gcggcagcca cgccacgaat tcggtcgagt ccttgaaatg cggcaggatg
      481 atggtggacg ccgtcaggtc tttcttgtgg gtgcccgggc tccacgccag catcgtcccc
      541 tcgagcgacc ggacccgggg cgccacgagg tcgatgtggg aatagccgcg ccgccatccg
      601 cgccaggtgc cgtactgctc gttgcgcgcg tcgatgcccc acgacttgta catccggacc
      661 agccagtcgt tggcccggcg ctggtccggt gagcccgtga gccgcggccc cagcgagtcg
      721 aagagggcat gggaaagagg ttcggtccac gagctgtcca tcccgatcga ccagatccgc
      781 ttgaggaccg ggtcgttggt gtcgtaggcc tgggcctggg cggtcatcgg caacaggagc
      841 gccgcgccga gggcgaacag gagggtcttt cgcatggctg gtctcgctgg ggtggggggg
      901 ggtggaagtc gcaggtcata ggtcacaggt cgcgggtcgc aggtcgcgca cacaacggca
      961 ggatcctggc cgttcgtgac ccgtgacccg cgacctgtga catacgtcct gcgacgtcac
     1021 ttcgctggca gcacccggat cttgatgttc ctgagttcca atcgcccgct gtggtcgccc
     1081 tggacgccga tcagcccgcg ggtcgccttc ccgtagttgg gccacttgcc gaacttgctc
     1141 gccttgacct tggcggccca gtcggcgctg cccaaggtat aggagaggag cttcttgccg
     1201 ttcatccagt gctccacatg gtcgccgcgc accacgatcc gggcggtgtt ccactcgccg
     1261 gcggggcgct cgatcccggc gggagaggga tacagcgcat agtcggcgcc ggccgaggtg
     1321 aggcggctgg ccccgtcggg ggcgttggcg tcgtcgagga gctggtactc gggcgcgctc
     1381 cagtagatgt ggtcgtactc ctcggtgccg cggtagaaga tccccgcatt cccgcccttg
     1441 gcgatcttcc aatccatcac cagctcgaag ttggcgaacg aatcgcgggt gacgatgtcc
     1501 tccgcgctcg aatccttgac gatgaggccg tcggcgacgc gccagccgac cggggccgcg
     1561 tcggtcttgt agccgcgcca cccatcgagc gacgtgccgt cgaagaggag gcgccagccg
     1621 tgcgcctttt ccttggcagt gagggtattg gcctgcgcgt gggccgcggg cgtggcggcg
     1681 aacgtgagcg aggccagcgc gatggcgccg gcgaaggcac ggatgttcat gggtcctctc
     1741 ggtggtgacg gcgaggccgc tcgaggcggc gaaggggccc acaagatgta acggcagcgg
     1801 gcgtgcggcg gaagcgccgg gcctcaggga acgacgatcg ggcccgcccg aggggggagg
     1861 cgctgccgcg ccggcatccc gtggccggtg aagccgtcgg gatccggccc ggacgcgcgc
     1921 atgccgctgt gaacggtgcg tgggacgtcc accgggaccg atgaaccggt cctcagtccg
     1981 gtttcacgga gaattcgatg cgccacggct agggtggacc ggccacggat cgcggaggtc
     2041 acggtgtcag ccaaggtctc gttacggatc gcgctcgtga tcagcatcgc cggcgcggcg
     2101 ttctccggag tgctcacctg gcgcgaactc gtctcgaggg tcgcaggagg atgctcggcg
     2161 gtcggcgcgc cggggacgat cttcggctac ccgccctgtg tgtacgggct cgtgatgtac
     2221 ctgttgctga tggtggtcac ggcgctggga ctccggtcgt cggaaggcgg gacgcggcca
     2281 cccgcgggct gatggccggc acccgagggc ccgagtaact tccccgcgtg acccgccgcg
     2341 ccaccctcct cgtcgtcctc gcgctcgtcc ctgcccgcgg cctcctggcc cagcggggcg
     2401 gggctcctta tcgcgacccg gcgctccccg tcgaagcccg cgtgcaggac ctcctccgcc
     2461 ggatgacgcc ggaggagaag ttctggcagc tctacatgag cccggtcttc ggcctccccg
     2521 gcgacaccgc caccgactat tcccacgggg ccttcggcct ccaggtcttc ggcgtcacca
     2581 ccccgcgcac caccgacgcc gcgcgcgcgt acgcggagcg ggtggacgcg ttccagcggt
     2641 accttgccga gcacagccgg ctcggcatcc cgatcatccc gttcgacgag gcggtgcacg
     2701 gcctgatgga ggccggcgcg accgactttc cggcggccat ctcgctcgcg gccacctggg
     2761 ataccgcgct ggtgacacgg gtggccggcg ccatcgccgt cgaggcgcgg agccggggcg
     2821 tccggcaggt gctctcgccg gtcatcaacg tcgccaccga cgtgcgatgg ggcagggtgg
     2881 aggagacgta cggcgaggac ccgctcctcg tctcccggat gggcgccgcc tacgtgggcg
     2941 ccttcgaacg cgccggcgtc gtcgccacgc ccaagcactt cgtggccaac gtcggcgcgg
     3001 gcgggcgcga cagctatccg atcgaggtga gcgatcgtga gctcgatcgg gtgtacttcc
     3061 cgccgttcga ggcggcagtg gcggcgggcg cccggtcggt gatgacagcg tacaactcgg
     3121 tggacggcga acccgcgagc cagaaccggc gcctcctgac cgaggtgctc aagggcgcgt
     3181 gggggt
//
LOCUS       DASUAP010000113         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1175210, whole genome shotgun sequence.
ACCESSION   DASUAP010000113 DASUAP010000000
VERSION     DASUAP010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175210"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1283)
                     /locus_tag="VFU45_02765"
     CDS             complement(39..1283)
                     /locus_tag="VFU45_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5358017.1"
                     /translation="MPFAEAIRLALASIWTAKLRSFFTLLGIIVSVAFLVAVTAIIQG
                     MNAYVKENLTGAMIGTNAFQVRRTPISIGLLDDEAIKALAKRPIISHDDADAVARALP
                     DARAVALQSGWPPPTGEVTYRGTTIGSVLIFGVTPPYQLVQDYRFEAGDPLADPDLRE
                     RRLVTVLGYDVAKKLFPDPADAVGRSVRVAGTEMTVRGVIAPKGQILGQSFDGFVLLP
                     LSTFEALYGRRKTTVVSVKMTDAAGVAPAMDRAEEAMRVAHHLRPGQPDDFTVDKADA
                     LLAFWQSLTKVLFTIIPAVVCIGIVVGGIVIMNIMLMTVNERIREIGLRKSLGARRRD
                     ILKQFLVETVALSMLGGLLGVAAGWGLAQLVAAVSPLPARVTPWAVGAALALGAGTGI
                     IFGVYPASRAARLDPITALRAE"
     gene            complement(1351..2613)
                     /locus_tag="VFU45_02770"
     CDS             complement(1351..2613)
                     /locus_tag="VFU45_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5358018.1"
                     /translation="MTKRRTLLDPARLAEGVGIAFDAVLSNKWRAFLTILGVAIGVAV
                     VISINAAIPGINRSVSQQLEQLGPKTFFVFRFFRGGLNISDGSDEMSPWRRNPWLKVD
                     EAEMLRKLPGIADVMYEEDTQGPVSAGSQKLASVDIAGMNPPWIYTTGGTIIEGRNYS
                     EVEEAAGAHVAVINDKLAQTLFPNLDALGRTIKVSGQPFEVVGIYSEPSALFSNADSP
                     KLAIPHSAFVKSVNYWKGWMNFAVLPKDSVSVGDAMDQVTAALRGRRGLKPAQDNNFS
                     LVTQDKLLDTFKSVTQGFFLVMIALSMVGLMVGGVGVVAIMMISVTERTREIGVRKAL
                     GATRQEILFQFLVEAATLTLIGCTVGMILGGAVTLGIRALTPLPAAISFTAVIIAIVA
                     AILTGVMFGMYPALKASRLDPVEALRYE"
     gene            complement(2610..>3053)
                     /locus_tag="VFU45_02775"
     CDS             complement(2610..>3053)
                     /locus_tag="VFU45_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5358019.1"
                     /translation="NFDLSSSENALAGWKKISGFLMMALPLLVGIGLVVGGIVIMNIM
                     LVSVTERTREIGVRKALGARHWDILFQFLVESATLSTVGAAMGIATGFALAFAFSALT
                     PLPAAVAPWSVIVGVGVGMGVGVIAGVYPASRAAKLDPIAALRTE"
BASE COUNT          495 a         1006 c         1113 g          439 t
ORIGIN      
        1 ggcgatgagc accccctcgc ggatggagga tccgcccatc attccgccct gagcgcggtg
       61 atcgggtcga gccgggcggc gcggctcgcc ggatacaccc cgaagatgat cccggtcccg
      121 gcccccaggg cgagcgcggc cccgaccgcc cacggggtca cccgcgcggg cagcggcgac
      181 accgccgcga cgagttgcgc cagtccccag cccgcggcga cgccgaggag cccgccgagc
      241 atcgagagcg ccaccgtctc caccaggaac tgcttgagga tgtcgcggcg gcgggcgccg
      301 agcgacttgc gcagcccgat ctcgcggatg cgctcgttca ccgtcatcag catgatgttc
      361 atgatgacga tgccgcccac cacgatcccg atgcacacga ccgccgggat gatggtgaag
      421 agcaccttgg tgaggctctg ccagaacgcc agcagcgcgt cggccttgtc gacggtgaag
      481 tcgtcgggct ggcccgggcg caggtggtgg gccacccgca tggcctcctc ggcgcggtcc
      541 atcgccggcg cgacgcccgc ggcatcggtc atcttgaccg agaccaccgt ggtcttgcgg
      601 cggccgtaga gcgcctcgaa ggtcgagagc ggcagcagca cgaagccgtc gaaggactgg
      661 cccaggatct ggcccttcgg ggcgatcacg ccgcgcacgg tcatctcggt gccggccacc
      721 cggaccgagc ggccgacggc gtccgcgggg tcggggaaga gcttcttggc cacgtcgtag
      781 cccaggaccg tcaccaggcg ccgctcccgg aggtcggggt cggcgagggg gtcgccagcc
      841 tcgaagcggt agtcctgcac cagctggtag ggcggcgtga cgccgaagat gagcacgctc
      901 ccgatcgtgg tcccgcggta ggtcacttcg ccggtgggcg gaggccaccc cgactggagc
      961 gccacggcgc gggcgtcggg gagggcgcgg gcgaccgcgt cggcgtcgtc gtgggagatg
     1021 atcggccgtt tggcgagcgc cttgatggcc tcgtcgtcga ggagcccgat gctgatcggc
     1081 gtgcggcgga cctggaaggc gttggtgccg atcatcgccc ccgtcaggtt ctccttgacg
     1141 taggcgttca tcccctggat gatcgcggtg accgcgacca ggaaggcgac cgatacgatg
     1201 atgccgagga gcgtgaagaa ggaccggagc ttggcggtcc agatggaggc gagggcgagc
     1261 cggatggcct cggcgaacgg catgcccgaa agctagcccg gtcgcctgaa gcggggccca
     1321 aggcacggtg aaggggaggg accgcgcccg ctattcgtag cggagcgcct ccaccgggtc
     1381 gagccggctg gccttgagcg cggggtacat cccgaacatc acgccggtca ggatggcagc
     1441 gacgatggcg atgatcaccg cagtgaacga gatcgcggcc gggagcgggg tgagggcccg
     1501 gatgccgagc gtcacggccc cgcccaggat catcccgacg gtgcagccga tgagggtgag
     1561 ggtcgccgcc tccacgagga actggaagag gatctcctgc cgggtggcgc cgagggcctt
     1621 gcggacgccg atctcacggg tgcgctcggt gaccgagatc atcatgatgg cgacgacgcc
     1681 gacgccgccc accatcaggc cgaccatcga cagcgcgatc atcaccagga agaacccctg
     1741 ggtgacgctc ttgaaggtgt cgaggagctt gtcctgggtc accagcgaga agttgttgtc
     1801 ctgcgccggc ttgagtccgc gccgcccgcg gagcgccgcg gtgacctggt ccatcgcgtc
     1861 ccccaccgac acgctgtcct tcggcagcac ggcgaaattc atccagccct tccagtagtt
     1921 cacggacttg acgaacgcgg aatgggggat ggcgagcttg ggcgaatcgg cgttgctgaa
     1981 cagcgccgac ggctcggaat agatgcccac cacctcgaac ggctgccccg agaccttgat
     2041 ggtgcggccg agggcgtcga ggttggggaa gagcgtctgt gccagcttgt cgttgatgac
     2101 cgcgacgtgg gcgccggccg cctcctcgac ctcgctgtag ttccgcccct cgatgatggt
     2161 gccgccggtg gtgtagatcc acggcgggtt catcccggcg atgtcgacgc tggcgagctt
     2221 ctggctgccg gcgctgaccg ggccctgggt gtcctcttcg tacatcacgt cggcgatgcc
     2281 cgggagcttc cggagcatct ccgcctcgtc gaccttgagc cacgggttcc ggcgccacgg
     2341 cgacatctcg tcggagccgt cgctgatgtt gagcccgccg cggaagaacc ggaagacgaa
     2401 gaacgtcttg gggcccagct gctccagctg ctgggacacg ctgcggttga tgccggggat
     2461 ggcggcgttg atcgagatga ccaccgccac gccgatggcg acgcccagga tggtgaggaa
     2521 ggcccgccac ttgttggaga gcaccgcgtc gaaggcgatg cccacgccct cggcgaggcg
     2581 cgccgggtcg aggagggtgc ggcgcttggt cattcggtcc tcagggcggc gatggggtcg
     2641 agtttggcgg cacggctggc agggtagacg ccggcgatga cgccgacccc catcccgaca
     2701 ccgactccga cgatcaccga ccagggggcg acggccgcgg ggaggggcgt cagggccgag
     2761 aaggcgaacg ccagggcgaa gccggtggcg atgcccatcg cggcccccac cgtggagagc
     2821 gtggcggact cgaccaggaa ctggaagagg atgtcccagt ggcgcgcgcc cagcgccttt
     2881 cggacgccga tctcccgggt ccgctcggtg accgagacga gcatgatgtt catgatcacg
     2941 atgccgccga ccaccagccc gatccccacc aggaggggaa gcgccatcat caggaacccg
     3001 gagatcttct tccagccggc cagcgcgttc tcggacgagc tcaggtcgaa gtt
//
LOCUS       DASUAP010000114         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_507248, whole genome shotgun sequence.
ACCESSION   DASUAP010000114 DASUAP010000000
VERSION     DASUAP010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507248"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2319
                     /locus_tag="VFU45_02780"
     CDS             <1..2319
                     /locus_tag="VFU45_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEU5358020.1"
                     /translation="LHNHPAGVLEPSTADLNVAARLHDAGVGFGIVNNDATELYVVVE
                     VPRARRLTPIDALDTAALLGEHGPVAGAMGRYEDRPSQRDMTAHIADGYNAGGVLLLE
                     AGTGVGKSFAYLVPAIRWAFANGERTVVSTNTINLQEQLVAKDLPLLREALREGEREP
                     RFALLKGWRNYLCLLRLQQAGQAQGSLLEPDKHQELVRLTAWAATTADGSLSDLPVPP
                     THEVWDEVSAEADLCPRLECPHFDRCFLFSARRRAAEADIVVVNHHLLAADLSVRQAA
                     NNWKDAAVLPPYRRLVLDEGHHLEDVAAAHLGAQVSSRGTRRLLARLERNGRGLLPAL
                     GQELSRNDDLLSQASLDLLRQRLLPAAGEARRAADALFLRLHARLDGESGGVLRLTDE
                     FPRDAIWTDGLGDELDRALGAFRRIREDLATIADRLHEAPASERRDQLLNECRAVARR
                     LEAVSDGLNVSLRPAAGGGARVRWMERAGRKGQEVTLASVPLDLAPVLRELLFDKLET
                     TVLTSATLAAGGEFDFLERRLGLSGESSPVTVREILPSPFDFPNQCLFGIPEDMPDPR
                     EDPAAHAAAVTDVVRDLAHASDGGMFVLFTSHASLKDQAARLRMVLGDRWPLLVQGEA
                     SRDALLQRFRDLGNAILLGTDSFWEGVDVPGRALRALVLTKLPFKVPGEPLTAARLER
                     LAEQGEDGFSGYLLPHAALKLKQGFGRLIRSSHDAGVVVLLDPRVVRKRYGRQVLHGL
                     PRAERVVGPWREVRTKCEDFFARHGVEGRAQS"
     gene            2514..>3003
                     /locus_tag="VFU45_02785"
     CDS             2514..>3003
                     /locus_tag="VFU45_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5358021.1"
                     /translation="MPIQPNARVNALPGYPLAEIPSIKRRLLEQGVDVIDMGAGDADL
                     MPPRPITDALVAALDELKYHKYGFQQGLPEFRQAIARFVERRFGQRFDALTETLPLVG
                     SKEGLAHLAFGVASPGDVVLVPEPGYQAYIGGALLSGSEPLICGLRREKDFLLDWDAI
                     PEA"
BASE COUNT          412 a         1078 c         1082 g          431 t
ORIGIN      
        1 ctgcacaacc acccggccgg cgtgctggag ccgtcgacgg ccgacctcaa cgtggcggcg
       61 cggctccacg atgccggtgt cgggttcggg atcgtcaaca acgacgccac tgagctctac
      121 gtggtcgtcg aggttccgcg cgcgcggcgg ctcacgccga tcgatgcgct cgacacggcc
      181 gcgctccttg gcgaacacgg cccggtggcc ggcgccatgg ggcgctacga ggaccggccg
      241 agccagcggg acatgactgc gcacatcgcc gacggctata acgccggtgg ggtgctcctg
      301 ctcgaggcgg gcaccggggt ggggaaatcg ttcgcctacc tggtgccggc catccgctgg
      361 gcatttgcca acggggagcg cacggtggtg agcaccaaca ccatcaacct ccaggagcag
      421 ctcgtcgcca aggacctgcc gctcctgcgc gaggcgctcc gtgagggcga gcgggagccg
      481 cgctttgcgc tcctcaaggg gtggcgcaac tacctctgcc tcctccggct gcagcaggcg
      541 ggccaggcgc agggctccct cctcgagccc gacaagcacc aggagctggt ccggctcacc
      601 gcctgggcgg ccacgacggc cgacggctcg ctgagcgacc tgcccgtgcc cccgacccac
      661 gaagtgtggg atgaagtgag cgcggaagcc gacctctgcc cccggctcga gtgcccgcac
      721 ttcgaccgct gtttcctgtt cagcgcccgc cgccgcgcgg ccgaggccga tatcgtggtg
      781 gtgaatcacc acctcctcgc cgccgacctg tcggtgcgtc aggccgcgaa caactggaag
      841 gacgcggcgg tgctgccgcc gtaccggcgg ctggtgctcg acgagggcca ccacctggag
      901 gacgtggcag cggcccacct tggcgcgcag gtgtcgagcc gggggacccg ccgcctgctg
      961 gcgcgcctgg agcggaacgg ccggggcctc ctcccggcgc tgggccagga gctgtcccgg
     1021 aacgacgatc tcctgagcca ggcgagcctc gacctcctgc gccagcggct cctgcccgcc
     1081 gccggcgagg cgcgtcgcgc cgcggacgcc ctcttcctgc gcctgcacgc ccggctggac
     1141 ggggagtcgg gcggcgtgct gcggctcacc gatgagttcc ccagggacgc gatctggacc
     1201 gacggcctcg gcgacgagct cgatcgggcg ctgggggcgt tccggcggat ccgcgaggac
     1261 ctcgccacca tcgccgaccg gctccacgag gcgccggcga gcgagcgccg cgaccagctc
     1321 ctcaacgagt gccgggccgt ggcgcgccgc ctggaagcgg tgtcggacgg gctcaacgtg
     1381 tcgctgcggc cggccgccgg tggcggcgca cgggtccggt ggatggaacg cgccgggcgg
     1441 aaggggcagg aggtgacgct cgcgtcggtg ccgctcgacc tcgcaccggt gctccgcgag
     1501 cttctcttcg acaagctcga gaccaccgtg cttacgagcg ccacgctcgc ggccggcggc
     1561 gagttcgact tcctcgagcg gcggctcggc ctctccggcg aatcgtcgcc ggtgacggtg
     1621 cgggagatcc tcccctcgcc gttcgacttc ccgaaccagt gcctcttcgg gatccccgag
     1681 gacatgcccg atccgcggga ggatcccgcg gcccacgccg cggcggtcac cgacgtcgtg
     1741 cgcgacctgg cgcacgcctc cgacggcggg atgttcgtgc tcttcaccag ccacgccagc
     1801 ctcaaggacc aggccgcgcg gctccggatg gtgctgggag accggtggcc cctgctggtg
     1861 cagggggagg cctcgcgcga tgcgctcctc cagcgcttcc gcgacctcgg caacgcgatc
     1921 ctcctcggca ccgactcgtt ctgggaaggg gtcgacgtgc ccggccgcgc cctccgtgcg
     1981 ctggtgctca ccaagctacc tttcaaggtg ccgggcgagc cgctcactgc cgcgcggctg
     2041 gagcgcctgg cggagcaggg agaggacggc ttcagcggat atctcctccc ccacgcggcg
     2101 ctcaagctca agcaggggtt cggccgcctc atccgttcga gtcacgatgc cggtgtggtg
     2161 gtgctcctcg acccgcgggt ggtccgcaag cgctacgggc gccaggtctt gcacggcctc
     2221 ccgcgtgccg agcgggtggt gggcccgtgg cgggaggtgc gcaccaagtg cgaggacttc
     2281 ttcgcccggc acggggtgga gggaagagct cagagctaag agctaagagc ttggagcttg
     2341 gagcttggac ctgcgagcgt agacatcaga gtttggagct ggcgctttgg ggggcggtcg
     2401 gggctcggag ctccgggccc ggcgggtcgg gattcgagac cggcaccacg cgcggctcgc
     2461 cacgtagtcc gcgacccgca tcacgcagtc ccacgatcaa cgacgactgt catatgccga
     2521 tccaaccgaa cgcccgcgtc aacgccctcc ccggataccc gctcgccgag atcccgtcga
     2581 tcaagcgccg cctgctcgag cagggcgtgg atgtgatcga catgggcgcc ggcgatgccg
     2641 acctgatgcc gccccgcccg atcaccgacg cgctggtggc cgcgctcgat gagctcaagt
     2701 accacaagta cggcttccag cagggcctcc ccgagttccg ccaggccatc gcgcggttcg
     2761 tggagcggag gttcggccag cgcttcgacg cgctcaccga gacgctcccg ctcgtcggat
     2821 cgaaggaggg gctggcccac ctggcgttcg gcgtggcctc gccgggggac gtggtgctgg
     2881 tgccggagcc ggggtaccag gcgtacatcg gcggtgcgct cctctcgggg tcggagccgc
     2941 tcatctgcgg gctccggcgc gagaaggatt tcctcctcga ctgggacgcg atccccgagg
     3001 cga
//
LOCUS       DASUAP010000115         3858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1066094, whole genome shotgun sequence.
ACCESSION   DASUAP010000115 DASUAP010000000
VERSION     DASUAP010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066094"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFU45_02790"
     CDS             <1..598
                     /locus_tag="VFU45_02790"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5358022.1"
                     /translation="GHSINTGNDDEDDGCPCGGTVRVALTVSGGTVTGIKTSVGGRWG
                     AGPAEELGQVGAAAAADYFLTLAERAPGEVGKRAILPAALADSAVVWRRLLGIAKDGR
                     VDRERRREATFWVGQAAESAATQGLDTLATDETGDREVREAAIFALSQRPKDEGIPAL
                     IQIAKTNRDPDLRKKALFWLGQSDDPRALKVFEDILGS"
     gene            697..3360
                     /locus_tag="VFU45_02795"
     CDS             697..3360
                     /locus_tag="VFU45_02795"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5358023.1"
                     /translation="MLITGLILGTILGAPGPRPKPDSTTTTHNAEVRAARTAARVTID
                     GALDDAAWQAATPVGDFLQRQPKEGAPASERTEVRVLYDDEAIYIGAHLYDDHPDSII
                     GRLGRRDADIQADKFTVFIDSYHDGRSGFYFGLNAAGTFYDGTLFNDEWDDNSWDGVW
                     QGAVQRTADGWTAEFRIPYSQLRFRNAQDLVWGIDFRRDIARKNENDWLVYQPLDGSG
                     FVSRFLPLRGLSGVRQATRLEVLPYVTTKATNAPYAAGDPFRAGSGSRLGAGADFKVG
                     VGGGLTLDGTVNPDFGQVEVDPAVVNLSDVETVFQEKRPFFIEGANIFDAFGQGGSNN
                     FWGFNWGGAEFFYSRRIGRAPQGSLPGNDYADVPTATTILGAAKLTGKLGTSWNVGAL
                     NAITGREDASYVSGGVRGTAEVEPFTYYGVYRAQKEIDGGRQGIGFIGTLVSRDLADS
                     ALRNELNSSALGGGIDGWTMLGHDRTWVLTGWFGGTDNRGSRADITALQQNSQHYFQR
                     PDARSVRVDSTATHLAGYMTRITLNKQKGDVQFNTAFGAISPGFDPQDAGLLWRANVL
                     NGHVMAGYRWSKPNAWRQQAQVNVAAFGSWDYDGNPVSKGVFGLVNLTLKNFWHVNLN
                     GAYNPETVDNRLTRGGPLALGPSGGEAFLNVNTNPQRSVVWTLSAGGGSYSQNSQRYW
                     NASLDATWQPLTTLSFELGPQYNWGRTGAQYVDTYADPTAAATYGSRYLFASLLQNEL
                     SANVRVNWIFSPKLSLQLYAQPLISSGQYSGYKALARPRSYAFDQYGQGAGSVTNYGD
                     SVVVDADGAGPSPAVNIGQPDFNFTSLRGNAVLRWEYLPGSTLYLVWTQSRANAVSAG
                     DFNVGPSWDRLVGTKPENIFALKVSYWWAPR"
     gene            3540..>3858
                     /locus_tag="VFU45_02800"
     CDS             3540..>3858
                     /locus_tag="VFU45_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358024.1"
                     /translation="MRRLLPALLLAAACGGPPPSAVPAVALTGATDSLTIPDLDIADA
                     APIGPDRWAILSQFGNVVRVVDWSRHTAAPLGRPGVDYVHPSSLTAVAGTLYVSDWGK
                     RALT"
BASE COUNT          609 a         1371 c         1356 g          522 t
ORIGIN      
        1 cggccactcg atcaacaccg ggaacgatga cgaggatgac ggctgtccct gtggcgggac
       61 ggtgcgggtg gcactcacgg tgagcggcgg cacggtgacg gggatcaaga ccagcgtggg
      121 cgggcgctgg ggcgccggcc cggcggagga actcgggcag gtcggcgcgg cggcggcggc
      181 ggactacttc ctgacgctcg cggaacgggc gccgggggag gtcgggaagc gggcgatcct
      241 gccggcggcg ctggcggaca gcgccgtggt gtggcggcgg ctcctgggca tcgccaagga
      301 cgggcgggtc gaccgcgagc ggcggcggga agcgacgttc tgggtgggtc aggccgccga
      361 gtcggccgcc acccaggggc tcgacaccct ggccaccgac gagaccggcg accgcgaggt
      421 ccgcgaggcg gcgatcttcg cgctctcgca gcgcccgaag gacgagggga tcccggcgct
      481 gatccagatc gccaagacca accgagaccc cgacctccgc aagaaggcgc tcttctggct
      541 cgggcagtcg gacgacccgc gggcgctcaa ggtgttcgag gacatcctcg gcagctgatt
      601 ccggccgcgc gttccacgcg cgcaacatgc aaccatttcc ggcgggccgt cgtctaagcc
      661 gcatcggacg gaccacgcac cgaaatggga gcagcgatgt tgatcaccgg attgatcctg
      721 gggaccatcc tgggcgcgcc cggaccgcgc ccgaagccgg acagcacgac gaccacgcac
      781 aacgccgagg tgcgggccgc acggacggcg gcgcgggtga cgatcgacgg tgcgctcgac
      841 gacgccgcgt ggcaggccgc gacgccggtc ggcgacttcc tgcagcgcca gccgaaggag
      901 ggcgccccgg catcggagcg caccgaggtg cgggtgctct acgacgacga ggcgatctac
      961 atcggcgcgc acctgtacga cgaccacccc gactcgatca tcgggcggct ggggcggcgc
     1021 gacgccgaca tccaggccga caagttcacc gtattcatcg actcgtacca cgacggccgc
     1081 agcggcttct acttcgggct caacgccgcg ggcaccttct acgacggcac gctcttcaac
     1141 gacgagtggg acgacaacag ctgggacggg gtgtggcagg gggccgtgca gcgcacggcg
     1201 gacggctgga cggccgagtt ccggatcccg tattcccaac tgcgcttccg gaacgcccag
     1261 gacctggtgt ggggcatcga cttccggcgg gacatcgcgc ggaagaacga gaacgactgg
     1321 ctggtgtacc agccgctcga cggaagcggc ttcgtctccc gcttcctgcc gctgcgcggc
     1381 ctgtcgggcg tgcggcaggc cacgcggctc gaggtgctgc cgtacgtcac caccaaggcc
     1441 accaacgccc cgtacgccgc cggcgacccg ttccgggccg ggtccggctc ccggctgggc
     1501 gcgggcgcgg acttcaaggt cggcgtcggc ggcgggctga ccctcgacgg caccgtcaat
     1561 cccgacttcg gccaggtcga ggtcgacccg gcggtggtca acctgagcga tgtcgagacg
     1621 gtgttccagg agaagcgccc gttcttcatc gagggcgcca acatcttcga cgccttcggc
     1681 caggggggct ccaacaactt ctggggcttc aactggggcg gcgcggagtt cttctactcc
     1741 cgccggatcg gccgcgcacc ccaaggctcg ttgccgggca acgactacgc cgatgtcccg
     1801 accgccacca ccatcctcgg cgccgccaaa ctgaccggca agctcggcac ctcgtggaac
     1861 gtcggcgcgc tcaatgccat caccgggcgg gaggacgcga gctacgtgag cggcggggtc
     1921 cgcgggacgg cggaggtgga gccgttcacg tactacgggg tctaccgggc ccagaaggag
     1981 atcgacggag gccgccaggg gatcggcttc atcggcaccc tcgtgagccg ggacctggcc
     2041 gattccgcgc tccggaacga gctcaacagc agcgcgctcg gcggcgggat cgacggctgg
     2101 acgatgctgg gccacgaccg cacctgggtc ctgaccggct ggttcggcgg caccgacaac
     2161 cgcggctcgc gggccgacat cacggcgctc cagcagaact cccagcacta cttccagcgc
     2221 cccgatgcgc ggagtgtccg ggtcgactcg accgccaccc acctggccgg gtacatgacg
     2281 cgcatcacac tcaacaagca gaagggcgat gtccagttca acaccgcctt cggcgcgatc
     2341 agccccggct tcgacccgca ggacgcgggg ctcctctggc gggccaacgt gctcaacggg
     2401 cacgtgatgg ccggctatcg gtggagcaag cccaacgcct ggcgccagca ggcacaggtc
     2461 aacgtggcgg cgttcggcag ctgggactat gacggcaacc cggtgtccaa gggcgtcttc
     2521 gggctggtca acctgacgct caagaacttc tggcacgtca acttgaacgg cgcctacaac
     2581 cccgagacgg tcgacaaccg cctgacccgc ggcgggccgc tggccctcgg gccatcgggc
     2641 ggcgaggcgt tcctcaacgt caacaccaac ccgcagcgct cggtggtgtg gacgctctcg
     2701 gccggcgggg gcagctactc gcagaacagc cagcgctact ggaacgcgtc gctcgacgcc
     2761 acctggcagc cgctcaccac gctctcgttc gagctcggcc cgcagtacaa ctggggacgg
     2821 acgggggccc agtacgtcga cacctacgct gacccgaccg ccgcggcgac gtacggcagc
     2881 cggtacctct ttgcctcgct cctgcagaac gagttgagcg ccaacgtgcg ggtgaactgg
     2941 atcttctcgc cgaagctgag cctccagctg tatgcccaac cactcatctc ctcgggccag
     3001 tacagcggct acaaggcact cgcccgtccc cggtcgtacg ccttcgacca gtacggccag
     3061 ggcgccggca gcgtcaccaa ctacggcgac tcggtggtgg tggacgccga cggcgcgggc
     3121 ccctcgccgg cggtcaacat cggccagccc gacttcaact tcacctcgct ccgcgggaac
     3181 gcggtgctcc gctgggagta cctgccgggc tcgaccctct acctggtctg gacccagtcg
     3241 cgcgcgaacg cggtgagcgc cggcgacttc aacgtcggcc cgtcgtggga ccggctggtg
     3301 ggcaccaagc cggagaacat cttcgcgctc aaggtctcgt actggtgggc gccgaggtag
     3361 ggggcgaccg ggtgggtggg cgcatcaggc gcgtaggcgc atgggcgggt cggcgcatgg
     3421 ggcgcgtggg cgaatggacg cgtggggcgg atggcggatg gtgggcggca catcccccgc
     3481 cggtgcaccc gccagccccc gccggtgcga ccatccgtcc atccgtctag ctttccgaca
     3541 tgcgccgact cctccctgcc ctcctcctgg ccgcggcgtg cggcggccca ccgccctcgg
     3601 cggtcccggc ggtggccctc accggcgcca ccgattccct cacgatcccc gacctcgaca
     3661 tcgcggatgc ggcgccgatc ggccccgacc gctgggccat cctctcccag ttcgggaacg
     3721 tggtccgggt ggtggactgg agccgccaca ccgcagcgcc ccttggccgc ccgggcgtcg
     3781 actacgtgca tccgtcctcg ctcacggcgg tggccggcac cctctacgtc agtgactggg
     3841 gaaagcgggc gctcaccg
//
LOCUS       DASUAP010000116         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_16594, whole genome shotgun sequence.
ACCESSION   DASUAP010000116 DASUAP010000000
VERSION     DASUAP010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16594"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..2097)
                     /locus_tag="VFU45_02805"
     CDS             complement(334..2097)
                     /locus_tag="VFU45_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358025.1"
                     /translation="MRELKTLIPYFRPYRRIYAAGLALVIVSNAFSTLVPRFVQHGID
                     AIAHQQGFALVLRAVELLLGAALLSGIARFGMRQYLNSVSRRVEYDLRNDLFAHLERQ
                     PAAFFDRHPTGDLMARATNDLLNIRMVAGPALMYLVDTVARTLLVLPAMLQISATLTG
                     YALLPLLGLPFGMIVFGRAIHARTEAIQEHFATITSHVMENLSGVRIVRAYRQEAAET
                     EAFARLSEEYVRRNLALVRAYGAFQPMLMGLGALGSVVVLLVGGRLVIAHAMSLGAFV
                     AFGVYLTMLVWPMIALGWVVNLVQRGAASMGRVNAILHEAPSITSPAEPARLPPAAGA
                     RALTFENVWFRYPGAEDRGWVLEDVSFTLAAGESLGVVGATGAGKSTLVDLVARTYDP
                     ERGRILVDGVDIRTLSLEDLRRTVSHVPQETFLFSESVRENVLFGMPDDGRLERASEA
                     AQLTEALPSLPDGYDTLLGERGVNLSGGQKQRTAIARALAKDAPIFLLDDALSAVDAS
                     TEARILKALRSALVGKTTVIVSHRLAAVRDATRIIVLDQGRIVETGRHPDLMRREGRY
                     WELVRRQELEVELEEEVKSEK"
     gene            2162..2878
                     /locus_tag="VFU45_02810"
     CDS             2162..2878
                     /locus_tag="VFU45_02810"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEU5358026.1"
                     /translation="MRKTIIGTLALAGALAACGGGAKGGQGQGDTLSRDLQLAPTDTG
                     SKLADRPARSAPPAARPAPSAPVLARGTSFSGAARDTITSRHNKPGESMVVLVSNDVK
                     DRSGRTVIPAGAPVTLHIDQLKSQSSRTARDSELHLTPVSVEIGGKSYPVSGTVDTIA
                     YQFVGRGITAGTAAKVGVGAAVGAVLGRVIGGNKTGTIVGGVAGAAGGAAVADQTGDR
                     DVTIYPGALVRMTLTGEFRR"
BASE COUNT          495 a         1055 c         1128 g          471 t
ORIGIN      
        1 ttctccagtg gaagggcgta gcgcttagag cttagagctt agcgcttaga gcttagagct
       61 tagcgcttag ggcttagggc ttagagctta gggcttggcg cttagggctt agggcttaga
      121 gcttagggac tagggcttgg cgcttagggc ttaggggtgg agtggtgtcg gagctcagcg
      181 ttccgtggcg ctcgcctcat acctctcacc cgtcttacct ctcacctctt acctcttacc
      241 tctcacatct tacctctcac atctcacctc tcacgtctca cctctcacgt ctcacctctc
      301 acgtcttacc tctcacctct cccttttcac ttctcacttc tcacttttca cttcctcttc
      361 cagctccacc tccagctcct ggcgccggac cagctcccag tagcgccctt cccggcgcat
      421 caggtcgggg tggcggccgg tctcgacgat gcggccctgg tcgaggacga tgatacgggt
      481 ggcgtcgcgc actgcggcca gccggtggga gacgatgacc gtcgtcttgc cgacgagcgc
      541 cgagcggagg gccttgagga tccgggcctc ggtgctcgcg tcgaccgcgg aaagcgcgtc
      601 gtcgaggagg aagatcggcg catccttggc gagcgcgcgg gcgatggccg tgcgctgctt
      661 ctggccgccg gagaggttga ccccgcgttc gccgaggagg gtatcgtagc cgtcggggag
      721 ggaggggagc gcctcggtga gctgggccgc ctcgcttgcg cgctccaggc gcccatcgtc
      781 gggcatcccg aagagcacgt tctcccggac cgactcgctg aagaggaacg tctcctgggg
      841 gacgtggctc accgtgcggc ggaggtcctc gagggagagc gtccggatat cgacgccgtc
      901 caccaggatc cggccacgtt cgggatcgta ggtgcgggcc acaaggtcga ccagggtcga
      961 cttgccggcc ccggtggcgc cgaccacgcc gagcgattcg ccggcggcca gcgtgaacga
     1021 cacgtcttcc agcacccagc cgcgatcctc ggcgccgggg tagcggaacc agacgttctc
     1081 gaacgtgagg gcgcgggcgc cggcagcggg gggcaggcgc gccggctccg cgggcgaggt
     1141 gatcgagggg gcctcgtgga ggatggcgtt gacgcggccc atcgacgcgg cgccgcgctg
     1201 caccaggttg accacccagc cgagggcgat catcggccag accagcatcg tgaggtagac
     1261 gccgaaggcc acaaaggcgc cgagcgacat cgcgtgggcg atgaccagcc ggccgccgac
     1321 cagcagcacc accaccgacc cgagcgcgcc caggcccatc agcatcggct ggaacgcgcc
     1381 atacgcccgg accagtgcca ggttccgccg gacgtactcc tcgctgagcc gggcaaaggc
     1441 ctcggtctct gccgcctcct gccggtacgc ccgcacgatc cggacgccgg agaggttctc
     1501 catcacgtgg ctggtgatcg tggcgaagtg ctcctggatc gcctcggtcc gggcgtggat
     1561 ggcgcgaccg aagacgatca tcccgaacgg gaggcccagg agcggcagca gggcgtagcc
     1621 ggtgagcgtt gcactgatct gcagcatcgc cgggaggacg aggagcgtgc gcgcgacggt
     1681 gtcgaccagg tacattagtg ccgggcccgc gaccatccgg atgttgagga ggtcgttggt
     1741 ggcgcgggcc atcaggtcgc cggtggggtg ccggtcgaag aatgcggccg gctggcgctc
     1801 gaggtgggca aagaggtcgt tgcgcaggtc gtactcgacc cgccggctca cgctgttgag
     1861 atactgccgc atcccgaagc gggcaatgcc gctgaggagc gcggcgccga gcaggagctc
     1921 gaccgcgcgg agcaccagcg cgaacccctg ctggtgggcg atcgcgtcga tgccgtgctg
     1981 gacgaagcgg ggcaccagcg tgctgaaggc gttggagacg atgacaagcg cgagccccgc
     2041 ggcgtagatg cggcggtagg ggcggaagta ggggatcagc gtcttgagtt cgcgcatcga
     2101 gggataaaag ctgtcccggt cgcagggccg ggacaagtca tcaccgatcc acggaggatg
     2161 tatgcggaag acgatcatcg gcacgctggc gctggcgggc gcgctggccg cgtgcggcgg
     2221 cggagcgaag gggggccagg ggcagggcga cacgctgtcg cgcgacctcc agctggcgcc
     2281 gaccgacacc gggtcgaagc tggccgaccg gccggcccgc agcgctcccc ccgccgccag
     2341 gccggccccg agcgcgccgg tcctggcccg cggcacctcg ttctcgggcg cggcgcgcga
     2401 caccatcacc agccgccaca acaagcccgg cgagtcgatg gtggtgctgg tctcgaacga
     2461 cgtgaaggac cggagcggcc ggaccgtgat ccccgccggc gcaccggtga cgctgcacat
     2521 cgaccagctc aagtcgcagt cgagccggac cgcccgcgac agcgaactcc acctgacgcc
     2581 ggtgagcgtc gagatcggcg ggaagagcta ccccgtctcc ggcaccgtgg acacgatcgc
     2641 gtaccagttc gtcgggcggg ggatcaccgc cggcaccgcg gccaaggtcg gcgtcggcgc
     2701 cgcggtgggc gcggtgctcg gtcgggtcat cggcggcaac aagaccggca ccatcgtcgg
     2761 cggcgtggcg ggggccgcgg gcggcgccgc cgtggccgac cagaccggcg accgcgacgt
     2821 cacgatctac cccggcgcgc tggtgcggat gacgctgacg ggagagttca ggcggtgaac
     2881 ggtgaacggt aaggggtgaa caggcgctcg tagccgaggc aggtgggtgt gaacagagaa
     2941 gggtggacgg gaccgtggaa ctcgccccgt tcacccttca cgccctcccg gtcaccgttc
     3001 actgctcacc gttcactgct caccgttcac gtcgtcctac tagtaccccg cccccccgcc
     3061 gccgagccgc gggtccgaga cgccctccca gccggtcggc gtgcggatga tcgcctcgac
     3121 gtcgccccac cgggtccgga acgcgatcg
//
LOCUS       DASUAP010000117         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_929946, whole genome shotgun sequence.
ACCESSION   DASUAP010000117 DASUAP010000000
VERSION     DASUAP010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929946"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VFU45_02815"
     CDS             <1..1035
                     /locus_tag="VFU45_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5358027.1"
                     /translation="ESLLHDHRPAGDREGLEAIREQAQRSRTIVRDLLAFVGRREERR
                     ESQAPGPLLERVVRGVSPELARHGAVLRTDIPADLPTIEVDAAGLEQVVTNLISNAAH
                     AAGRGGKVVLSARAGHFDALEIVVSDTGPGIPPDVFPRLYEPFFTTKAPGEGTGLGLS
                     VSLGIVEHHGGTIRAENAPDGGARFIVTLPRGLRGSPAPLRLVAGAGADASLPTDHGR
                     SRVLLVEDEQPVRLALRRYFEKFGWAVDEASDGAQGLARLLAAGAEREYDVVVCDLKM
                     PGLSGIELYERLTAAKSPLLGRLIFATGDVASPEAAAFLARTTHPVLEKPFELAQLAL
                     AVRSVMQPRG"
     gene            1028..2245
                     /locus_tag="VFU45_02820"
     CDS             1028..2245
                     /locus_tag="VFU45_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HEU5358028.1"
                     /translation="MADGPAPVVVIGAGAAGIVAAGHAARRAPVVLLERTRDGGRKIL
                     ISGGGRCNILPSSMAPEQYVTASSPNIVRNILRGWPLEAQRTFFERDVGVPLKLEQES
                     GKLFPVSDKARDVRDGLLAWAGRQGARPRFDAVVTGLEPAGGAWRVALDNGETIAAAK
                     VILASGGLSVPQTGSDGTGLRILRALGHTIHDTYPALTPLTLDPPAYAGLAGVSLDVT
                     IRAGEGRGAFVTTGGFLFTHRGYSGPAVLNVSHLAVRARLARLPPPEITVQWTPLNAD
                     QWRVELGGGAARVGSVLKRRLPERLALVLLAEAAVDADRELSQLRREERERVITALTS
                     WPLRSTSDEGYKKAEVTGGGVALDEIVPRTMESRLHRGLFICGEALDCFGPIGGYNFL
                     WAWVTGRAAGLGA"
     gene            2555..3004
                     /locus_tag="VFU45_02825"
     CDS             2555..3004
                     /locus_tag="VFU45_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5358029.1"
                     /translation="MFTLTNRPLWLDRDLGHIDRLLNEVSGVFGRQSGDATDAATWIP
                     AVDVFESKDELRIVAELPGVRPEDVKLSVENGMLAIRGEKKQVAEEGAEAVHRYERSY
                     GAFLRSFRIPGTVDAERIQARYEHGVLTVTLPKAEKAKAREIAVQVR"
     gene            complement(3071..3625)
                     /locus_tag="VFU45_02830"
     CDS             complement(3071..3625)
                     /locus_tag="VFU45_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358030.1"
                     /translation="MSLLILLALLVPGAARQQPFLQYGGMFPGVAWKAVTPYIAAKAP
                     RRAACRATTEDAPATVIRTHTCLVRRLLLTNDVRANVAFTVDDSSGKVASIIIAYRPS
                     RDAGREAALDTLTRQFGPPVAADTARGAAKWTQGSKSLDLTTRPLPGTTENGVTAPIV
                     VILADADLRVAIDARVKGAGPVKR"
     gene            complement(3622..>3941)
                     /locus_tag="VFU45_02835"
     CDS             complement(3622..>3941)
                     /locus_tag="VFU45_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5358031.1"
                     /translation="LRKAVEAVHAGDSFFSPVIARRLSEAERGQLDREARSGSLAQLT
                     GRERDVLAGIARGLTNKEIAAELGISHRTVETHRESLMRKLRIHTVAGLTKFALETGV
                     TSA"
BASE COUNT          540 a         1383 c         1449 g          569 t
ORIGIN      
        1 gagtcgctgc tccacgacca ccgcccggcg ggcgaccggg aagggctcga ggccatccgg
       61 gagcaggccc agcgctcacg caccatcgtg cgcgacctgc tcgcgttcgt cgggcgccgg
      121 gaggagcgtc gggagagcca ggccccgggc ccgctcctcg aacgggtggt gcgcggcgtt
      181 tcccccgaac tggcccgcca cggcgccgtg ctgcgcaccg acatcccggc cgacctgccc
      241 accatcgagg tcgacgccgc cggcctcgag caggtggtga ccaacctcat ctccaacgcc
      301 gcgcacgccg ccggccgcgg cggcaaggtg gtgctgagcg cccgggccgg ccacttcgac
      361 gcgctcgaga tcgttgtgag cgacaccggt cccggcatac cgccggacgt gttcccgcgc
      421 ctctacgagc ccttcttcac caccaaggcg ccgggcgagg gcaccgggct cgggctctcg
      481 gtgtcgctcg gcatcgtgga gcaccacggc ggcaccatcc gggccgagaa cgcacccgac
      541 ggcggcgccc gtttcatcgt caccctgccc cgcggcctcc gggggtcgcc ggcgccattg
      601 cgcctcgtgg ccggggccgg cgcggacgcg tcgctcccca ccgaccacgg ccggtcgcgg
      661 gtgctgctgg tggaggacga gcagccggtc cggctcgcgc tgcggcgcta cttcgagaag
      721 ttcggctggg cggtggatga ggcgtccgat ggggcgcagg ggctggcgcg gcttctggcg
      781 gccggcgccg agcgggagta cgacgtggtc gtctgcgacc tcaagatgcc cggcctctcc
      841 ggcatcgagc tctacgagcg gctcaccgcc gcgaaatcgc cgctcctcgg gcgcctgatc
      901 ttcgccaccg gcgacgtggc ctcacccgag gcggccgcct tcctggcccg caccacgcac
      961 ccggtgctcg agaagccgtt cgaactggcg cagctggcgc tcgccgtgcg cagcgtgatg
     1021 cagccgcgtg gctgacggcc cggcgccggt cgtggtgatc ggcgccggcg ccgcgggcat
     1081 cgtcgccgcc gggcacgcgg cccgccgtgc tccggtcgtc ctgctcgagc ggacccgcga
     1141 cggcggccgg aagatcctca tcagcggcgg cggccgctgc aacatcctgc cgtcgagcat
     1201 ggcccccgag cagtacgtca ctgcgtcctc gcccaacatc gtccgcaaca tcctccgcgg
     1261 atggccgctc gaggcccagc ggacattctt cgagcgcgat gtcggcgtgc ccctcaagct
     1321 ggagcaggag agcggaaagc tcttcccggt ctccgataag gcccgcgacg tccgcgatgg
     1381 cctgctggcc tgggccgggc gccagggggc ccggccacgc ttcgacgccg tcgtcaccgg
     1441 actcgagccc gcaggcggcg cgtggcgggt ggcgctcgac aacggcgaga cgatcgccgc
     1501 cgcgaaggtg atcctcgcct cgggcgggct ctcggtgccg cagaccggga gcgatggcac
     1561 cggactccgg atcctcaggg cgctgggcca cacgatacac gacacgtatc cggccctcac
     1621 gccgctcacg ctcgatcccc ccgcctacgc cgggctcgcc ggcgtgtcgc tcgacgtgac
     1681 gatccgcgcg ggcgagggcc gcggcgcctt cgtgacgacc ggcggattcc tcttcaccca
     1741 tcggggctac agcggacccg cggtgctcaa cgtttcccat ctcgcggtcc gcgcccggct
     1801 ggcgcgcctg ccgccgccgg agatcaccgt gcagtggacg ccgctcaatg cggaccagtg
     1861 gcgcgtcgaa ctgggcggcg gcgcggcacg ggtcgggagc gtcctcaagc ggcgcctgcc
     1921 ggagcggctc gcgctcgtgc tcctggccga agcggccgtc gatgccgacc gcgaactttc
     1981 ccagctgcgg cgcgaggagc gcgagcgggt gatcacggcg ctcaccagct ggccgctccg
     2041 ttccaccagc gacgagggtt acaagaaggc ggaggtcacc gggggcggag tggcgctcga
     2101 cgagatcgtg ccgcgcacga tggaaagccg gctccatcgg ggactcttca tctgcggcga
     2161 ggcgctcgac tgcttcggtc ccatcggcgg ctacaacttc ctgtgggcgt gggtgacggg
     2221 cagggcggcg gggcttggag cgtagagcgt ggagcttaga gcttggagcc tggagcttag
     2281 agcttggggc ttggagcttg gagcttagag gttggtgcgt atagtcggga gtgcagttct
     2341 gatagctgat agctgatagc ctctcccccg tcccccctcc cctccgccaa actgacaatc
     2401 ccctttgcca gccccgcggt cgatcctgtc agatcgaccg aaatgggcgt gcatgccagt
     2461 tgctcaccgg ggggtgccga gccgaccggg tcactggtcg gcgatccaca tatccagaac
     2521 cgcggccttc ggccgccgaa cgacgaggag ccctatgttc acgctgacca atcgcccgct
     2581 ctggctcgac cgcgacctcg gtcacatcga ccgcctgctc aacgaggtga gcggcgtctt
     2641 tggccgccag tccggcgacg cgaccgacgc cgccacctgg atccccgcag tggacgtctt
     2701 cgagtccaag gatgaacttc ggatcgtggc cgaactgccg ggtgtccggc cggaggacgt
     2761 gaagctctcg gtcgagaacg ggatgctcgc gatccggggg gagaagaagc aggtggccga
     2821 ggagggcgcc gaggcggtgc accgctacga gcgaagctac ggcgcgttcc tgcggagctt
     2881 ccgcatcccg gggacggtgg atgccgagcg gatccaggcc cggtatgagc acggggtgct
     2941 taccgtgacg ctgccgaagg cggagaaggc caaggcgcgg gagatcgcgg tccaggtgcg
     3001 gtaagccggc tgccgctgga tggaggccgg gctcctggtc ggtggaacga ccgggagccc
     3061 ggccgtgtct tcagcgcttg acggggccgg cgcccttgac gcgggcgtcg atcgcaacgc
     3121 gcaggtcggc gtcggccagg atgacgacga tcggcgcggt gacgccgttc tccgtcgtgc
     3181 ccgggagcgg gcgggtggtc aggtcgaggg acttggaccc ctgcgtccat ttggccgcgc
     3241 cgcgagcggt gtcggccgcc accggcggcc cgaactggcg cgtgagcgtg tcgagggcgg
     3301 cctcccgtcc ggcatcgcgc gaggggcggt aggcaatgat gatcgaggcg accttgccgg
     3361 aggaatcgtc gacggtgaac gccacgttgg cgcgcacgtc gttggtcagc aggagccggc
     3421 ggaccaggca ggtgtgggtc cggatgaccg tcgccggcgc gtcttccgtc gtggcacggc
     3481 aggccgcgcg gcgcggcgcc ttcgctgcga tgtagggggt gaccgccttc cacgcgaccc
     3541 cggggaacat cccgccgtac tggaggaacg gctgctgccg cgccgcgccg ggaacgagca
     3601 gcgccagcag gatgagcagg ctcatgccga ggtcactccc gtctcgagcg cgaacttggt
     3661 gaggccggcc acggtgtgga tccggagctt ccgcatcagg ctttcgcggt gggtctcgac
     3721 ggtgcggtgg ctgatgccga gctccgcggc gatctccttg ttggtgagcc cgcgcgcgat
     3781 cccggccagc acgtcgcgct cacggccggt gagctgggcg agcgaccccg agcgtgcctc
     3841 acggtcgagc tggccgcgct ccgcttccga caggcgccgg gcgatgaccg ggctgaagaa
     3901 cgagtcgccg gcgtggaccg cctccaccgc cttgcggagg t
//
LOCUS       DASUAP010000118         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1011505, whole genome shotgun sequence.
ACCESSION   DASUAP010000118 DASUAP010000000
VERSION     DASUAP010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011505"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFU45_02840"
     CDS             complement(<1..232)
                     /locus_tag="VFU45_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358032.1"
                     /translation="MDSILQDLRYAARRLLRTPAFTITAVLTLAIGIGAVTAVFSLVD
                     GVLLRSLPFPAADRLMNVFAGGTAPLGVYRVAS"
     gene            complement(313..2742)
                     /locus_tag="VFU45_02845"
     CDS             complement(313..2742)
                     /locus_tag="VFU45_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5358033.1"
                     /translation="MDSILQDLRYAARTLAKAPGFTATAILTLALGIGATTGVFSVID
                     AALLRPLPFRAPERLAVLEHTWGPPPAGFPKMSLDLTDWRAHPEIFDQVGSYSTGGMN
                     LAAGSGAERVKTALVTTTFFDLLGARPALGRAFGTDEGEPGRGGVVILSDGLWRTRFG
                     GDHGVLGRTVTLNERPFVVIGVMPRGFGYPRQSEVWIPRSIPEDMKNWDMFHSSVVET
                     VIGRVRGGLTFRDAQVRADGVSRAFRQDHPLPPGATDARMTLTPFRDVLVGDSRPGLL
                     VLFGATGLILLIACSNAAGLLLARAARRQHEVAVRAVLGATRTRVVRQMLTESGLLAL
                     AGAGAGLLLAWWLQGSLAALVPARLADVAPVTMDLRVLVFAAAGATLATVLFGLAPAV
                     GLARTDLQSSLRAGGYRSWGVRARRLRGGIVAAEVGLALTLLVGAGLMLKSLYLLTHV
                     DPGFDREGVLTATVSLPSARYRAPGGFTAVYRRLLGRFQALPGVTAVGTVNAPPLLRE
                     GAIGLAFDIVGRPPFPMDADRPFAEQLVVSPGYFAALRIPVIRGRVFTAADDSGAPPV
                     AIINASMARRYWPQGDAIGARIMSLGGRGGPLTVVGVVSDVEDGDLGDSTWQSQIYTP
                     YEQNTPAYLTFAIRAALPPAELAASVRRAAAEADPTVPLYDIRTMEQVVETSIAPERS
                     RSLLIGVSGLLAVLLAGVGVYGVMAQVVSQRTREIGVRMALGASGRSVLGLVVRSGLW
                     LTGVGVVAGLAAAWALSRVLGSLLYQVSVTDASVFVGAPAVLCLVALAACYFPARRAS
                     RVDPVVALRTE"
     gene            complement(2820..>3147)
                     /locus_tag="VFU45_02850"
     CDS             complement(2820..>3147)
                     /locus_tag="VFU45_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5358034.1"
                     /translation="IYGLMAYLVQQRTREFGIRMALGARQQDVLGMVLRHGAVLVGVG
                     VIAGIAGAMLATRLIANQLFGVGKFDAVTFASVSLLLVVIALAATWLPAYRATRVDPV
                     EALRTE"
BASE COUNT          559 a         1159 c         1044 g          385 t
ORIGIN      
        1 cactggcgac tcgatagacc ccgagagggg cggtcccacc ggcgaaaacg ttcatgagtc
       61 gatcggccgc cgggaagggg agggacctga gcagcacgcc gtcgacgaga ctgaacaccg
      121 cggtgacggc cccgatcccg atcgccaacg tgaggacggc cgtgatcgtg aacgccgggg
      181 tgcgaagcaa gcgccgcgcg gcgtagcgga gatcttggag tatcgagtcc atggacggct
      241 ccttatgagg gatcaggggc tagggatcag ggatcagtga actactgacc cctaacctct
      301 gacgcctggt ccctattccg ttctcagcgc caccaccgga tccactcggc tcgcccggcg
      361 cgcggggaag taacacgccg ccagggccac caggcacaac accgccggcg cgcccacgaa
      421 caccgatgcg tcggtgacgc tcacctggta cagcaggctc cccagcacgc gcgagagcgc
      481 ccaggccgcc gccagacccg ccaccacgcc gaccccggtc agccagagcc ccgaacgcac
      541 caccaacccg agcaccgaac ggccgctcgc gccgagggcc atgcgcacgc cgatctcccg
      601 cgtccgctgg ctgaccacct gcgccatgac cccgtagacc ccgacgccgg ccaggagcac
      661 cgccagaagc ccggacacgc cgatcaggag cgagcgggag cgctcggggg cgatggacgt
      721 ctccaccacc tgctccatgg tccggatgtc gtacaacggg accgtcgggt ccgcctcggc
      781 cgccgcgcgc cgaaccgatg ccgcgagttc cgcgggcggg agcgccgcac gaatcgcgaa
      841 cgtcaggtac gcaggcgtgt tctgttcgta gggcgtgtag atctggctct gccaggtgga
      901 gtcgcccaga tcgccatcct cgacgtcgga caccactccc accaccgtca ggggtccccc
      961 gcgcccgccg agcgacatga tccgggctcc gatggcgtcg ccctggggcc agtacctccg
     1021 ggccatcgag gcgttgatga tggccacggg cggagctcca gaatcgtcgg cggccgtgaa
     1081 cacgcgcccc ctgatcacgg ggatgcgcag cgccgcgaag tacccgggcg acaccaccag
     1141 ctgctcggca aagggccggt ccgcatccat gggaaagggg ggacgcccca cgatgtcgaa
     1201 ggccagcccg atggctccct cccgaaggag cggcggtgcg ttcacggttc ccaccgccgt
     1261 gacgcccggc agcgcctgga accggccgag caggcgccgg tacacggccg tgaaccctcc
     1321 cggtgcccgg taccgcgccg agggcagact caccgtggcg gtcagcacgc cctcgcggtc
     1381 gaatcccgga tcgacgtgcg tgaggagata gaggctcttc agcatgagcc ccgcgcccac
     1441 gagcagcgtc agcgcgagcc ccacctccgc cgccacgatg ccgccgcgga gccggcgggc
     1501 tcgcacaccc caggaccggt atccgcccgc gcgcagggag ctttggagat ccgtccgtgc
     1561 cagaccgacc gcgggcgcga gcccgaacag cacggtcgcg agggtggcgc cggcggcggc
     1621 gaagacgagc acccggaggt ccatcgtcac cggagccacg tcggccagcc gggccggcac
     1681 cagcgccgcc agcgagccct gaagccacca ggcgaggagc aggccggcgc ccgctcccgc
     1741 gagcgccagc agcccgcttt ccgtcagcat ctgccgcacc acgcgggttc gcgtggcgcc
     1801 cagcaccgcc cgcacggcca cctcatgctg ccgccgcgcg gcccgcgcga ggagcaggcc
     1861 ggccgcgttg ctgcaggcga tcaggaggat gagccccgtc gcgccgaaga gcacgagaag
     1921 cccgggccgg gagtcgccga ccagcacgtc gcggaacggt gtgagggtca tccgggcgtc
     1981 cgtcgccccc ggaggcagcg ggtggtcctg ccggaaggcc cggctcacgc cgtcggcacg
     2041 gacctgggca tcccggaagg tgagaccacc ccggacccgt ccgatcaccg tctcgacgac
     2101 ggacgaatgg aacatgtccc agttcttcat gtcttcgggg atcgaccgcg gaatccagac
     2161 ttccgactgg cgcggatacc cgaaccctcg cggcatgacg ccgatcacca cgaagggacg
     2221 ctcgttgagc gtgacggtgc ggcccagcac gccgtgatca ccgccgaacc gggtgcgcca
     2281 gagcccatcg ctcaagatca ccacgccgcc ccggcccggc tctccctcgt ccgtgccgaa
     2341 ggcgcgtccg agcgccggcc gtgcgccgag caggtcgaag aaggtggtgg tcacgagagc
     2401 ggtcttgacc cgctccgcgc ccgatcccgc ggcgaggttc atgccgccgg tggagtacga
     2461 tccgacctga tcgaagatct ccggatgggc ccgccagtcg gtgaggtcga gactcatctt
     2521 cgggaagccc gccggcggcg gtccccaggt gtgctcgagc accgccagcc gctcgggggc
     2581 ccgaaagggc agaggcctga ggagcgccgc gtcgatcacg ctgaagactc ccgtggtcgc
     2641 gccgatgccc agggcgaggg tgaggatggc tgtcgccgtg aacccgggcg ctttggcgag
     2701 cgtccgcgcg gcgtagcgga gatcttggag aatcgagtcc atggacggct ccttgaagga
     2761 tcaggggcta gggatcaggg atcagtgaac tactgaccct aacccctgac ccctggtccc
     2821 tattctgttc tcagtgcctc caccggatcc actcgcgtcg cgcgataggc cggcaaccag
     2881 gtcgctgcca gagcgatcac aaccagcaac agagacaccg acgcgaacgt gacagcatcg
     2941 aacttcccaa ccccgaacag ctgattggcg atcagcctcg tggcgagcat cgccccggcg
     3001 atcccggcga tcacaccaac ccccaccaac accgctccat gccgcaacac cattcccaac
     3061 acgtcctgct gacgcgcgcc cagggccatc cggatcccga actcgcgggt ccgctgttgg
     3121 acgagatacg ccatcaagcc gtagatc
//
LOCUS       DASUAP010000119         6182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1107184, whole genome shotgun sequence.
ACCESSION   DASUAP010000119 DASUAP010000000
VERSION     DASUAP010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107184"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFU45_02855"
     CDS             <1..478
                     /locus_tag="VFU45_02855"
                     /inference="COORDINATES: protein motif:HMM:NF027969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU5358035.1"
                     /translation="RIGDTLSTGPALEFQGIPRFPPEHFARITCGDPLRRKRMDAGLR
                     QLTEEGAAQVFFTTGSDVSGPTPIVGAVGMLQFDVMLHRLEHEYGVRCALEPIAARYP
                     RWVTGPMAAIERLAEAPGRVLLFDAKGAPLLLFADEWTLRHTMSTESGLQFHDVAP"
     gene            468..1385
                     /locus_tag="VFU45_02860"
     CDS             468..1385
                     /locus_tag="VFU45_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5358036.1"
                     /translation="MRRDRPTPARPPVGDYLLRALIADAPGVTILLLTVLLALGSILA
                     GLLGALTGLGGGIIIVPLLTLGFGVDIRYAVGASLISVIATSSGAAAAYVKEGYTNIR
                     VGMLLEMGTVAGALVGAWLAGVVSTHVIGTVFGLILLWSAVNSLRPHPDHGSDGPAHP
                     WAMKLRLNSTFPTPEGARHYTVQGVPAGLALMVVAGILSGLLGIGSGIIKVLAMDQTM
                     KLPFKVSTTTSNFMIGVTAAASAGVYLHRGYIEPTLAVPVMLGVLAGALLGARLLTLA
                     NVRHLRTLFTLVLVVFAVQMLYKSLSGAL"
     gene            1382..1762
                     /locus_tag="VFU45_02865"
     CDS             1382..1762
                     /locus_tag="VFU45_02865"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEU5358037.1"
                     /translation="MTEKQLEGHDPMELIIGNILRWGVIVAAVVGSLGAVGELIIRGS
                     AIPEFRRFTGDAATGLPAIYHGVVQGHSRAIMQLGIALLIATPIVRVGLSLVAFAREK
                     DRTYVIITSMVLLLLLYGLLGGKV"
     gene            1762..2610
                     /locus_tag="VFU45_02870"
     CDS             1762..2610
                     /locus_tag="VFU45_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358038.1"
                     /translation="MARIAFVTSQALHDLTPSDRLAAAALRARGADVVPAVWTDPTVR
                     WEGFDRVVVRSPWDYFAHQDRFLDWFDTLDCAGVPVENPTSILRWNLDKIYLRELDIP
                     VVATEWVAQAGTLDLPAILRRRDWRRAVIKPTISAGGHDTWTVDAGNAAAIGERMAPV
                     LGRCGLMIQPFVEQVRTRGEISFLFFRKEFSHAVLKKPKAGEFRVQVEYGGSAEAFTP
                     SPALVTQAERVVRAVKGDLLYARVDGVDVDGEFQLMELEVLEPDLFLGHSPRAPERFA
                     EAVLAR"
     gene            complement(2721..4031)
                     /locus_tag="VFU45_02875"
     CDS             complement(2721..4031)
                     /locus_tag="VFU45_02875"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEU5358039.1"
                     /translation="MQAIARAALAAVAPGPALRAALTGLPARPKRPPHILAFGKAAQP
                     MATAAVAVLAEWGMAPGGGVVIAPETGPAPHPALPVAIGDHPVPGIHSRAAALALGQA
                     VRAVQRGDEVWVLLSGGATSLVAAPIDGIGVEDFEWLFSRLATSGLDIRAMNRIRKRF
                     ARWGAGRLAAALAGASIRLFVVSDVVGDDLLSIGSGPCVPDPSTAAEIRAELEATPLW
                     PDLPAALRAHLAQVEAGKLPETPKPEAPVFDAVTGEIISSNRAAVLGAARKAEELGFE
                     TVVNTKPLVGNAADAGRKVAEWVLTRPPVSKRPGERPWCMIWGGETTVNLTGPHGHGG
                     RCQELALAAAERLAAHGTRPGLRVLALGTDGRDGPTDAAGGVVDAETWGKIKAAGIDP
                     AVALAGHDAYPALDAAGALVRLGLTGTNVMDVVIALELAGGERA"
     gene            4239..5408
                     /locus_tag="VFU45_02880"
     CDS             4239..5408
                     /locus_tag="VFU45_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5358040.1"
                     /translation="MPGRHFLFVPGPTNVPDRILSALHVAMEDHRSSAFPALWRSIAP
                     QLGRLFGTTHGRSFVYPGTGTGGWEAALTNVLSPGDRVLAPRAGQFSHLWIELARQLG
                     LEVEVLDCEWGTGAPADRIEAALKADHGHAIKALLIVHNETATGVTSDVAAIHRAQLA
                     ARHPSLLLVDSVSGIGSLEFRMDEWGVDVAVTGSQKGLMLPAGLAILCASPRVLDAGA
                     TATSRRAYFDFAAAAQANDQGYFPYTPSAPMLRALRESLTMLLDEEGMPAVIARHHRL
                     AEGVRRAVAAWGLRLCAREPRWHSDTVSTILTPDGIDARAVISRAFDRYDLSLGAGLG
                     PLAGRAFRIGHLGDLNELMLHGALAGVEMALQDCGVTLTPGSGVAAAEEYWRHQA"
     gene            5405..>6182
                     /locus_tag="VFU45_02885"
     CDS             5405..>6182
                     /locus_tag="VFU45_02885"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEU5358041.1"
                     /translation="MTRPVITVTRKLPAEVELELSRAFDARLNELDVPLTPDALQRAL
                     RESDGLICTVSDRLDAAALGLGPLRCRILANYGVGFNHIDVDAARALGITVTNTPGVL
                     TEATADLAIALMLMVTRRAGEGERLVRAGQWTGWRPTQLLGASLAGKTLGIVGMGRIG
                     REVARKAQAAWGMDISWHSRSAGEDGPPPIAGARWVPLDELLGSADVVSLHVPATPAT
                     RHLIDARRLGLMKPTAYLINTARGDVVDEAALGAALRSGRI"
BASE COUNT          818 a         2313 c         2113 g          938 t
ORIGIN      
        1 ccgcatcggt gacacgctct ccaccggccc tgcgctcgag ttccagggga tcccgcgctt
       61 cccacccgag cactttgccc ggatcacctg cggcgacccg ctccgccgca agcggatgga
      121 cgcgggcctc cggcagctca cggaggaagg ggcggcgcag gtgttcttca ccaccggatc
      181 ggacgtgtcg gggccgacgc cgatcgtcgg cgcggtgggg atgctccagt tcgacgtgat
      241 gctccaccgg ctcgagcacg agtacggggt gcgctgcgcc ctcgaaccga tcgccgcgcg
      301 ctatccgcgc tgggtcacgg ggccgatggc ggccatcgaa cggctggccg aggcgcccgg
      361 ccgcgtgctc ctgttcgatg caaagggcgc cccgctgctg ctctttgccg acgaatggac
      421 cctgcggcac accatgagca ccgagtcggg actccaattc cacgacgttg cgccgtgacc
      481 gcccgacgcc tgcgaggccg ccggtcggcg actatcttct ccgcgccctc atcgcggacg
      541 cgcccggcgt gacgatcctc ctcctcacag tcctcctggc cctcggctcc atcctcgccg
      601 gcctcctcgg cgcgctcacc gggctcggcg gcggcatcat catcgtcccg ctcctcacgc
      661 tcggattcgg cgtcgacatc cgctacgccg tcggcgcctc gctcatctcg gtcatcgcca
      721 cctcctccgg tgccgccgcg gcgtacgtga aggagggcta caccaacatc cgcgtcggga
      781 tgctgctcga gatgggcacc gtggccggcg cgctggtggg cgcctggctg gccggggtcg
      841 tatccaccca cgtgatcggg accgtcttcg gtctcatcct cctctggtcg gccgtcaact
      901 cgctccgccc ccatcccgac cacggcagcg acgggccggc ccatccgtgg gccatgaaac
      961 tccggctcaa ctcgacgttc cccacccccg agggcgcccg gcactacacg gtgcagggcg
     1021 tccccgccgg cctcgcgctg atggtggtgg ccggcatcct ctcgggcctc ctcggcatcg
     1081 ggtcgggcat catcaaggtg ctggcgatgg accagacgat gaagctgccg ttcaaggtct
     1141 ccaccacgac cagcaacttc atgatcggcg tcaccgcggc cgcgagcgcc ggcgtctacc
     1201 tccaccgggg ctacatcgag cccacgctgg cggtgcccgt gatgctcggc gtgctcgccg
     1261 gcgccctcct cggcgcccgg ctcctgaccc tcgcgaacgt gcgccacctg cggacgctct
     1321 tcaccctggt gctcgtggtg ttcgcggtgc agatgctgta caagtcgctc tcgggtgccc
     1381 tgtgacggag aagcagctgg aaggccacga cccgatggag ctcatcatcg ggaacatcct
     1441 ccgctggggc gtcatcgtcg ccgcggtggt gggttccctc ggcgccgtgg gcgagctcat
     1501 catccgcggc agcgccatcc cggagttccg ccgcttcacc ggcgacgccg ccaccggcct
     1561 cccggccatc taccacggcg tggtgcaggg gcactcccgc gccatcatgc agctcggcat
     1621 cgccctcctc atcgccaccc cgatcgtccg ggtggggctc tcgctcgtcg cctttgcccg
     1681 ggagaaggac cggacctacg tgatcatcac ctcgatggtc ctgctccttc tcctctacgg
     1741 cctcctcggc ggcaaggtct gatggcgcgc atcgcgttcg tcacctccca ggcgctccac
     1801 gacctcaccc ccagcgaccg gctcgccgcc gccgcgctcc gcgcccgcgg cgcggacgtg
     1861 gtgccggcgg tgtggaccga ccccaccgtc cggtgggagg gcttcgaccg ggtggtggtc
     1921 cgctcgccgt gggattactt cgcccaccag gatcgcttcc tcgactggtt cgacaccctc
     1981 gactgcgcgg gggtcccggt cgagaacccg acctcgatcc ttcgttggaa cctcgacaag
     2041 atctatctcc gcgagctcga catccctgtc gtagccaccg aatgggtggc acaggcggga
     2101 acgctcgacc tgccggcgat cctccgtcgc cgcgattggc gccgggcggt gatcaaaccc
     2161 acgatctcgg cgggtgggca cgacacctgg acggtggatg cagggaacgc ggccgcgatc
     2221 ggcgagcgga tggcgccggt cctcggtcgc tgcggcctga tgatccagcc cttcgtggag
     2281 caggtccgga cccggggaga gatctccttc ctcttcttcc ggaaggagtt cagtcacgcg
     2341 gtgctcaaga agcccaaggc gggagagttc cgggtccagg tggagtacgg cgggagtgcg
     2401 gaggcgttca ccccctcgcc ggcgctcgtg acgcaggcgg aacgggtcgt ccgggcggtg
     2461 aagggagatt tgctctacgc ccgcgtggac ggcgtggacg tcgacgggga gttccagctg
     2521 atggagcttg aggtgctgga accggacctc ttcctcggcc actcgccccg cgcgccggag
     2581 cgctttgccg aggcggtgct ggcgaggtag ggagcgggca gtaagcgggc ctagagcggg
     2641 caataagcgg gcgtggagcg ggccaagagc gggaatcgtg cccgcgtgtc cgcccactac
     2701 atgcccgctg tccgcccgct ctacgcccgc tctccccccg ccagctccag cgcgatcacc
     2761 acgtccatca cgttggtccc ggtgaggccg agccggacca gggcgcctgc cgcgtcgagt
     2821 gccgggtagg cgtcgtggcc ggcgagcgcc accgccgggt cgatccccgc cgccttgatc
     2881 ttcccccacg tctccgcatc caccacgccg ccggcggcat ccgtcggacc gtcccggccg
     2941 tcggtgccga gtgccaacac ccggagtccg gggcgggtgc cgtgcgcggc cagccgttcg
     3001 gcggcggcga gcgccagctc ctggcaccgg cctccgtggc cgtggggccc cgtcaggttc
     3061 accgtcgtct cgccccccca gatcatgcac caggggcgct cccccgggcg cttggacacc
     3121 ggcggtcggg tcagcaccca ttcggccacc ttgcggcccg cgtccgcggc attgccgacg
     3181 aggggcttgg tattcaccac ggtctcgaag ccgagctcct ccgccttgcg ggcggcgccg
     3241 agcactgcgg cgcggttgct ggagatgatc tcgccggtga ccgcgtcgaa gaccggcgct
     3301 tccggcttcg gcgtctccgg cagcttgccg gcctcgactt gggccaggtg ggcgcggagt
     3361 gcggcaggaa gatcgggcca gagcggggtg gcttcgagct ccgcgcggat ctcggcggcg
     3421 gtggagggat cgggaacgca ggggccggag ccgatcgaca ggaggtcgtc gcccaccacg
     3481 tcggagacga cgaagaggcg gatcgatgcc cccgcgagcg ccgccgccag ccgccccgcg
     3541 ccccagcgcg cgaaccgctt gcggatccgg ttcatcgccc ggatgtcgag cccgctggtg
     3601 gccaggcggg agaagagcca ttcgaagtcc tcgaccccga tcccgtcgat cggcgcggcg
     3661 accaggctgg tggcgccgcc ggaaaggagg acccagacct cgtcgcctcg ctggaccgcg
     3721 cgcaccgcct gaccgagcgc gagcgcggcg gcgcgggagt ggatgccggg caccgggtga
     3781 tcgccgatcg cgaccggcag ggcggggtgg ggtgcggggc cggtctcggg cgcgatgacc
     3841 acacccccgc caggcgccat tccccattcc gcgaggaccg ccactgcggc cgtggccatc
     3901 ggctgcgccg ccttcccgaa ggcgaggatg tggggcgggc gcttcggacg ggcggggagg
     3961 ccggtgagtg cggcgcggag cgcggggccc ggcgccacgg cggcaagcgc cgccctggcg
     4021 atcgcctgca tatggtcgcg cggagtaccg gaaggcgtcg gagtgctcac gctggcccgc
     4081 cccccgctgg gacttcgatc acctcatcat ctcctcttcg catcgccaca cccgtcaccc
     4141 tcccgattcc cctcttccga tttccctctt cccctttccc ttttcccccc gcaccaagca
     4201 acttacccga acccctccaa ccccgcgacc atcgccagat gcccggacgc cacttcctgt
     4261 tcgttcccgg tccgaccaac gtccccgacc ggatcctgag cgccctgcat gtggcgatgg
     4321 aagaccaccg gtcgtcggcg ttcccggcgc tctggcggtc gatcgccccg caacttggcc
     4381 ggctcttcgg cacgacgcac gggcggtcat tcgtctatcc cggcaccggg accggggggt
     4441 gggaggcggc gctcaccaac gtgctgtccc ccggcgaccg ggtgctcgcg ccccgcgccg
     4501 gccagttcag ccacctctgg atcgagctgg cgcgccagct cggactcgag gtcgaggtgc
     4561 tcgattgcga gtggggcacc ggcgcgccgg ccgaccggat cgaggcggcg ctcaaggccg
     4621 accacgggca cgcgatcaag gcgctcctca tcgtccacaa cgagacggcc accggcgtca
     4681 cgagcgacgt cgcggccatc caccgcgcgc aactcgccgc tcgccacccg tcgctcctcc
     4741 tggtcgactc ggtgagcggc atcgggtcgc tcgagttccg gatggacgag tggggcgtcg
     4801 atgtggcggt gaccgggtcg cagaaggggc tgatgctccc cgccgggctc gccatcctgt
     4861 gcgcctcgcc gcgggtcctc gacgcgggcg ccaccgccac ctcccgccgc gcctatttcg
     4921 actttgccgc cgccgcccag gccaacgacc aggggtactt tccctatacg ccgtcggcgc
     4981 cgatgctccg ggccctgcgg gaatccctca cgatgctcct cgatgaagag gggatgccgg
     5041 cggtcatcgc gcggcaccat cgcctggccg aaggggtccg ccgcgcggtg gcggcgtggg
     5101 gcctcaggct ctgcgcacgg gagccgcgtt ggcactcgga caccgtctcg accatcctca
     5161 cgcccgacgg gatcgatgcc cgcgccgtca tatcccgcgc gttcgaccgg tacgaccttt
     5221 cgctcggcgc cggactcgga cccctcgccg gcagggcctt ccggatcggc catctcggcg
     5281 acctgaacga gctgatgctc catggcgccc tggccggggt ggagatggcg cttcaggact
     5341 gcggcgtgac gctcacgccc gggtcggggg tcgccgcggc ggaagagtac tggcggcacc
     5401 aagcgtgacc cgcccggtca tcaccgtcac ccggaagctc cccgcggagg tcgaactcga
     5461 gctctcgcgt gctttcgacg cgaggctcaa tgagctcgat gtgccgctca cgccggatgc
     5521 cctccagcgg gcgctccgcg aaagcgacgg cctcatctgc accgtgagcg accgcctcga
     5581 tgcggcggcg ctgggactcg ggcccctccg gtgccggatc ctggccaact acggcgtcgg
     5641 cttcaaccac atcgatgtgg acgcagcccg cgcactcggc atcacggtca ccaacacgcc
     5701 cggcgtcctg accgaggcca ccgccgatct cgcgatcgcc ctgatgctca tggtcacacg
     5761 gcgcgcgggg gagggggagc gtctcgtccg cgccgggcag tggaccggct ggcggccgac
     5821 ccagctcctc ggcgcaagcc tcgcgggcaa gacgctcggc atcgtcggaa tgggccgcat
     5881 cgggcgggag gtggcccgca aggcgcaggc cgcctggggc atggacatca gctggcattc
     5941 gcggtcggcg ggcgaggatg ggccgccgcc gatcgccggc gcgcgatggg tgccgctcga
     6001 cgagctcctg ggctcggccg acgtcgtgtc gctccacgta ccggcgaccc cggccacccg
     6061 gcacctgatc gatgcgcgcc gcctcggact gatgaagccc accgcgtacc tcatcaatac
     6121 cgcccgtggc gacgtggtcg acgaagcggc gctcggcgcg gcgttgcgat cggggcgcat
     6181 cg
//
LOCUS       DASUAP010000120         3689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1338544, whole genome shotgun sequence.
ACCESSION   DASUAP010000120 DASUAP010000000
VERSION     DASUAP010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338544"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VFU45_02890"
     CDS             complement(<1..987)
                     /locus_tag="VFU45_02890"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEU5358042.1"
                     /translation="MPGTSLAYRGAVRIATTLLPVAAAFDAKLRRGHDARRGALARLR
                     AWAARERDRSRPLLWFHAPSVGEGLQAEAVLRRIRERHPEWQLAYTHFSPSAEPLARR
                     VGASVADYLPYDTPAAARQLLDALAPTALIFSKLDLWPELATSAAARGIPVALIAATV
                     RPGSGRLRWPARALLAPGYRALRAVAAVDRPDADRLIALGAPRDAVHVLGDPRFDSVA
                     AKVAAVAPDDRLLRFGQGAPTLVAGSTWPGDESALLDAFATVRARRPDARLILVPHEP
                     TERHLAATETWARRAGLPAPTRLSHADGATPFLLVDRVGVLAALYGAGSVAYV"
     gene            1067..2212
                     /locus_tag="VFU45_02895"
     CDS             1067..2212
                     /locus_tag="VFU45_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5358043.1"
                     /translation="MPSTRAADAAGPGGRALSRLLDDGRFFLPGPTEVRPEILAALAG
                     PMISHRGAAFERLFAALQAGLGPAFRTTRPVLISSSSATGLMEAGVRSAPAGPILALV
                     NGAFAERFVTVARACGREVEELAADWGRVVPLDAVEARLKRGRFAAVTVVHSETSTGA
                     LTDVRAVQELARRYGAVCLVDSVSGVAGIPVETDLWGLDYVFTGSQKAFAIPPGLAFA
                     VASESFLAGAAKVPGRGRYFDLVEFDAFARRSQTPNTPALPLLYALEAQLAAMAAEGI
                     EARWARHEAMREHVSRWAGAAAASGVPVGVLAAEGERSATVSALTLPAGVPASAVVAQ
                     LEGRGYVVGGGYGKLRETTIRIGHMGDHTVTGLERCLAALGGILGVR"
     gene            2209..2934
                     /locus_tag="VFU45_02900"
     CDS             2209..2934
                     /locus_tag="VFU45_02900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEU5358044.1"
                     /translation="MNYWQLSRSPRYSVLFAAPLLLAYELTARVLTHGDAGVRNGADV
                     MLKSLFVWLGGRDGILLFDLLLAAVGIALIWRDRRAGGAPLRWRVFALMLGEAVVLAV
                     AFGFVASLLTALILNGPSILAIQHRAAFDVPTQLMVSLGAGIYEELLFRVLIVSGLVV
                     MGRRVFGWGPGWSNAFAVVIGAVVFSAFHYIGPYGDPLQLPSFVFRAVAGLLFSGLYV
                     LRGYGVTAWTHSLYDIFLTIATR"
     gene            complement(3168..>3689)
                     /locus_tag="VFU45_02905"
     CDS             complement(3168..>3689)
                     /locus_tag="VFU45_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5358045.1"
                     /translation="KAPITVYEMSDFQCPFCRKFFLETFPTLEKEYITTGKVRWIFIN
                     LPIPQLHPNAEPAAEFAMCSARFGKFWLVHDLLYHHQDTWAPLKEPGTFLLTLADSVR
                     IPRDSLLPCLQHHDMEARVGGDAASAARIGAKATPSFLVDGALLTGAYPIEVFRHVLD
                     SIYVVRTAGRSEK"
BASE COUNT          475 a         1283 c         1343 g          588 t
ORIGIN      
        1 gacatacgcg accgagcccg caccgtagag cgccgccagc acgccgaccc ggtccaccag
       61 caggaacggc gtggcgccgt ccgcgtggct gaggcgcgtc ggcgccggga ggcccgcccg
      121 acgagcccac gtctccgtcg cggcaagatg gcgctcggtc ggttcgtgcg gcaccaggat
      181 gagtcgcgcg tctgggcgtc gggcccgcac ggtggcgaac gcgtcgagca gtgccgactc
      241 atctcccggc cacgtcgatc ccgccaccaa ggtcggcgcg ccctggccga accgaaggag
      301 ccgatcgtcc ggcgccaccg cggcgacctt tgccgccacg ctgtcgaatc ggggatcgcc
      361 gagcacgtgg acggcgtcgc gcggggcgcc gagggcaatc aggcggtccg cgtcggggcg
      421 gtcgaccgcg gcgacggcgc ggagggcgcg gtaccccggg gcgaggagcg cgcgcgccgg
      481 ccagcggagg cggccgctgc cgggtcgcac ggtcgctgcg atgagcgcca ccggtatgcc
      541 gcgcgccgcc gcgctggtgg cgagctccgg ccagaggtcg agcttggaaa agatgagcgc
      601 tgtcggcgcc agggcgtcga ggagctgccg cgcggcggcg ggggtgtcgt atgggaggta
      661 gtcggccacc gacgcaccga cccgccgggc gagcggctcg gccgacggac tgaaatgggt
      721 gtacgcgagc tgccactccg gatgccgctc gcggatgcgc cggagcaccg cctccgcctg
      781 gagcccttcg cccacggatg gcgcgtggaa ccagaggagc gggcggctgc ggtcacgctc
      841 ccgcgcggcc caggcgcgga gacgcgccag cgccccccgc cgggcgtcgt gcccgcgccg
      901 gagcttggcg tcgaacgccg ccgccaccgg gaggagcgtg gtcgcgatcc ggacggcgcc
      961 acgataggcg agggatgtgc cgggcatcgg cgaaagctcg aagggcgcgc ggcgtccgtc
     1021 aacgtccttg ctaccgcccc acgatccgac ctcctacatt gcgccgatgc cttcgacgag
     1081 ggccgcggac gcggcagggc cgggcggacg ggcgctgagc cgcctgctcg atgacggacg
     1141 attcttcctc cccgggccca ccgaggtgcg ccccgagatc cttgctgcgc tcgccgggcc
     1201 gatgatctcc catcgcgggg ccgcgttcga gcggctcttc gcggcgctgc aggcgggact
     1261 cggccctgcc ttccggacca cccgtcccgt cctcatcagc tcgtcgtccg ccaccggcct
     1321 gatggaggcg ggcgtgcgct cggccccggc cggaccgatc ctggcgctcg tgaacggcgc
     1381 cttcgccgag cgcttcgtga cggtggcgcg ggcgtgcggg cgggaggtcg aggaactcgc
     1441 ggcggactgg ggccgggtgg tgccgctcga cgcggtcgag gcccggttga agcgcggacg
     1501 cttcgccgcg gtgaccgtcg tgcattcgga gacctcgacc ggcgccctca ccgacgtgcg
     1561 ggcggtccag gaactggccc gacgatatgg cgccgtctgc ctcgtggaca gcgtctccgg
     1621 cgtcgccggg atcccggtcg agaccgatct gtggggcctc gattacgtct tcacggggtc
     1681 ccagaaggcc ttcgccatcc cgccgggcct cgcctttgcc gtcgcatctg agtcgttcct
     1741 ggccggcgcc gcgaaggtcc cgggccgggg gcggtacttc gacctggtcg agttcgacgc
     1801 ctttgcgcgc cgttcgcaga cgcccaacac gccggcgctc cccctcctct acgcgctcga
     1861 ggcccagctc gccgcgatgg cggcggaggg catcgaagcg cgctgggcgc ggcacgaggc
     1921 gatgcgtgag cacgtctcgc ggtgggccgg ggccgcggcc gcatccggcg tcccggtggg
     1981 cgtccttgcc gccgaggggg agcgctcggc cacggtctcg gcgctcacgc tcccggccgg
     2041 ggtgccggcg tcggcggtcg tcgcccagct cgagggacgg ggctacgtgg tgggcggcgg
     2101 gtacgggaag ctgcgggaaa cgaccatccg catcggccac atgggcgatc acaccgtcac
     2161 ggggctcgag cgctgcctgg ccgcgctcgg cgggatcctg ggggtgcggt gaattattgg
     2221 cagctgagcc ggagcccccg gtactcggtg ctgttcgcgg cgccgctcct cctcgcctac
     2281 gagctcacgg cccgggtcct cacccacggc gacgccggcg tgcgcaacgg cgccgacgtg
     2341 atgctcaaga gcctcttcgt ctggctcggc gggcgggacg ggatcctgct gttcgacctc
     2401 ctgctggccg cggtcgggat cgcgctcatc tggcgcgatc gccgcgcagg cggggcgccg
     2461 ctccgttggc gtgtcttcgc cctgatgctg ggcgaggcgg tcgtgctcgc ggtggcgttc
     2521 ggattcgtcg cgtcgctcct caccgcgctc atcctgaacg ggccgtccat cctggccatc
     2581 cagcaccgcg cggcgttcga cgtgccgacg cagctgatgg tgtccctcgg cgccggcatc
     2641 tacgaggagc tgctcttccg cgtgctgatc gtatcggggc tcgtggtgat ggggcggcgg
     2701 gtcttcggct ggggcccggg gtggtcgaat gcgttcgcgg tggtgatcgg ggcggtggta
     2761 ttctcggcgt tccactatat cgggccctac ggcgacccgc tgcagctgcc gtcgttcgtc
     2821 ttccgtgccg tggcgggcct cctcttcagc gggctctacg tcctccgcgg gtacggcgtc
     2881 accgcgtgga cccactcgct gtacgacatc tttctcacga tcgcgacgcg gtgagggggt
     2941 gggggtgaga cgtgagacgt aagacgtgag acgtcagacg ttagacggaa gaggcttcgg
     3001 atgatgccaa cgaccggcat atgcacaagg ggctccccaa tgaacggagc ccctcttagc
     3061 tgttgcctct tacccctcac ctctcacctc gtacttccca cttcttactt ctcacttctc
     3121 acttcttact tctcacttct cacttcttac ttcttacttc ttacttctca cttctcactt
     3181 cttcccgccg tgcgcaccac gtagatcgaa tcgagcacgt gccggaacac ctcgatcgga
     3241 taggcgccgg tcaggagcgc gccgtcgacc aggaacgacg gcgtcgcctt ggcgccgatg
     3301 cgggccgcgc tcgcggcatc ccccccgacg cgggcctcca tgtcgtggtg ctgcaggcag
     3361 ggaagcagtg agtcccgtgg gatgcggacg gagtcggcca gggtgagcag gaaggtcccc
     3421 ggctccttga gcggcgccca ggtgtcctgg tgatggtaca gcaggtcgtg caccagccag
     3481 aacttcccga accgggccga gcacatcgcg aactccgcgg ccggctcggc gttcggatgg
     3541 agctggggga tgggcaggtt gatgaagatc caccgcacct tcccggtcgt gatgtactcc
     3601 ttttcgagcg tcgggaacgt ctccaggaag aatttccggc agaacgggca ctggaagtcc
     3661 gacatctcgt agacggtgat gggcgcctt
//
LOCUS       DASUAP010000121         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_3554, whole genome shotgun sequence.
ACCESSION   DASUAP010000121 DASUAP010000000
VERSION     DASUAP010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3554"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VFU45_02910"
     CDS             <1..1253
                     /locus_tag="VFU45_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5358046.1"
                     /translation="FNFEAGVRPERQDFLVNINPESLLLEGARRVDEWSLIEKKIPSF
                     DLIFSLDQPHLAGSNVQLSSEQERLVPLLDGRHDVHQLVEDSGLVEFEVGKALYGLIT
                     AGFAHRVGTSTGVVEPNVSAARIDEHRNLGIAFYKTGMLDEALREFRRVADLRASEAS
                     APFYVGLIALKQAKWAEGVDSLKQALERGGPRPAALYNLAYGLERMGKFDEAEAAYGE
                     AVTRARDDARMMTGWGIAALKRGDAGVASGRLQRARELWGAKVPPAIWFWAAALAHAV
                     ESDLENAITVAQEGVTAYPASAVLKNDLAVLLELTGDVAGAEAMVRDALGEDPSVPQL
                     SKNLGDLLYRAGKYEEADEAYQRAAKLAPALGDDLYFKLGNIAFRQRDTGRARAHWQR
                     VVELNPNHQLARSNLESMSSEAAG"
     gene            1250..2074
                     /locus_tag="VFU45_02915"
     CDS             1250..2074
                     /locus_tag="VFU45_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEU5358047.1"
                     /translation="MMLTGDDAGFAALTRTISATAGLALDGYKEKCLRRRIAVRMRAC
                     GVHTFADYQRLLAAEPAEYEKLTDALTINVTRFYRNAETWDRLARDVLPGMWGGRQGA
                     VRVWSAGCSSGEEPYTLAMLLAAEAERSARRSWLERSTIVATDIDKICLERARAGRYP
                     EAAFQETPAALTARYTTPVPGGREVVPELRRIVDIRRVDLFLDPVPGPAFDLITCRNV
                     IIYFDRDSQERLFTRFVDALLPGGILVLGKVETLFGPARDRLELVDVRERIFRRPA"
BASE COUNT          321 a          762 c          793 g          306 t
ORIGIN      
        1 ccttcaactt cgaggcgggg gtccgtccgg agcgtcagga cttcctggtc aacatcaacc
       61 ccgagtcact gctcctcgag ggcgcccgcc gggtggacga gtggagcctg atcgagaaga
      121 agatcccgtc cttcgacctg atcttctccc tcgaccagcc gcacctggcc ggctcgaacg
      181 tccagctctc atcggagcag gaacggctgg tgccgctgct cgacggtcgc cacgacgtcc
      241 accagctggt ggaggacagc ggcctggtgg agttcgaggt cggcaaggcg ctctacggcc
      301 tcatcaccgc ggggttcgcg catcgggtgg gcacctcgac cggcgtggtc gagcccaacg
      361 tcagcgcggc gcggatcgac gagcaccgca acctggggat cgcgttctac aagaccggga
      421 tgctcgacga ggcgctgcgc gagttccgcc gcgtggccga cctccgcgcc tcagaggcga
      481 gcgccccgtt ctatgtgggg ctcatcgcgc tcaagcaggc caagtgggcg gagggcgtcg
      541 actcgctcaa gcaggcgctg gagcggggcg gcccgcggcc cgccgccctc tacaacctgg
      601 cgtacggcct ggagcggatg gggaagttcg acgaggccga ggcggcctac ggcgaggcgg
      661 tgacccgggc gcgggatgac gcccggatga tgaccggctg gggcatcgcg gcgctcaagc
      721 ggggcgatgc cggggtggcg tcggggcgcc tgcagcgcgc gcgcgagctc tggggcgcca
      781 aggtgccgcc ggccatctgg ttctgggccg cggcgctggc ccacgcggtc gagtccgacc
      841 tggagaacgc gatcaccgtg gcccaggaag gggtcacggc gtatcccgcg agtgccgtgc
      901 tcaagaacga cctcgccgtg ctcctcgaac tgaccggcga cgtggccggc gcggaggcga
      961 tggtccgcga cgcgctcggc gaggatccgt cggtgccgca gctctcgaag aacctgggcg
     1021 acctcctcta tcgcgccggg aagtacgagg aggccgacga ggcgtaccag cgtgccgcca
     1081 agctggcgcc cgccctcggc gacgacctct acttcaagct ggggaacatc gccttccgcc
     1141 agcgtgacac cggccgcgcc cgcgcccact ggcagcgggt ggtggagctc aacccgaacc
     1201 accagctggc gcggagcaac ctggagtcga tgtcctcgga ggccgcggga tgatgctgac
     1261 cggggatgac gccggcttcg ccgcgctgac gcggacgatc tccgcgaccg cgggcctcgc
     1321 cctcgacggg tacaaggaga agtgcctgcg gcggcggatc gcggtgcgga tgcgcgcctg
     1381 cggggtgcat accttcgcgg actaccagcg gctcctggcc gcggagcccg ccgagtacga
     1441 gaagctgacc gacgcgctca ccatcaatgt cacccgcttc taccggaacg ctgagacgtg
     1501 ggaccgcctg gcccgcgacg tcctgcccgg gatgtggggt ggccgccagg gggcggtccg
     1561 ggtctggagc gccgggtgtt cctcgggcga ggagccgtat accctcgcga tgctgctggc
     1621 ggccgaggcg gagcggagcg cccgccggtc gtggctcgaa cggagcacca tcgtggccac
     1681 cgacatcgac aagatctgcc tcgagcgcgc acgcgccggc cggtatcccg aggcggcctt
     1741 ccaggagacg ccggctgcgc tcacggcccg ctacacgacg ccggtgccgg gagggcggga
     1801 ggtcgtgccc gagcttcgcc ggatcgtcga catccgccgc gtcgacctct tcctcgaccc
     1861 cgtgccgggg ccggccttcg acctcatcac ctgccggaat gtgatcatct acttcgaccg
     1921 cgacagccag gaacgcctct tcacccgctt cgtcgacgcc ctcctgcccg ggggcatcct
     1981 ggtgctgggc aaggtggaaa cgctcttcgg gccggcgcgc gaccggctgg aactggtcga
     2041 cgtgcgcgag cggatcttcc ggaggcccgc gtgaccgcgc cggacgccgt catcgtcaag
     2101 gtggccgaac tgcgggcggc gggcgccgac acggagctcg ccaccctcgg tctcggaagc
     2161 tgcgtcgcga tcatcctgca cg
//
LOCUS       DASUAP010000122         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_357713, whole genome shotgun sequence.
ACCESSION   DASUAP010000122 DASUAP010000000
VERSION     DASUAP010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357713"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VFU45_02920"
     CDS             complement(<1..286)
                     /locus_tag="VFU45_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU5358048.1"
                     /translation="MRVTTWTEYSLIITLHLAKRATGDGTPVAARELAEAERLPGDYV
                     EQILLRLRRAGLVESVRGARGGYYLARDASRISVRDVMTASEHQTFEMNCD"
     gene            complement(351..1769)
                     /locus_tag="VFU45_02925"
     CDS             complement(351..1769)
                     /locus_tag="VFU45_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEU5358049.1"
                     /translation="MATRIEKDPLGEKPVPADALYGIQTLRASENFPISGLRPLPLFV
                     DAVVWIKKAAALTHKETGRLEARLADAIVRAADEVLGGQHRDQFIVDVYQAGAGTSHN
                     MNCNEVLANRANELLGAPRGAYAPVHPNDHVNMAQSTNDVIPTAMRLATLASLPRLLT
                     ALDGLGAAFLAKGKAFDGIIKSGRTHLQDATPIRLGQEFTAYGHTVLRHRAKLAEAAR
                     WLRAMNIGGTAVGTGINAEPQYPALMVKHLRAATGLDLEVAEDRIQLMQSMGDIASFS
                     GALRAWVLDLNKIANDIRLLASGPRTGLAEILLPAVQPGSSIMPGKVNPSIAEMVNQV
                     CYQVLGLDTTVALAAEAGQLELNVMMPVITHNIVFAMQILTNAVTVFAERCVVGIEAD
                     AAQCEHWLERSPALVTALAPKIGYAESAKLAKEALATGLTVKQLVVKKGLLQGKELEE
                     VLDLRAMTELGVPGGKGIPGGG"
     gene            complement(1860..>2582)
                     /locus_tag="VFU45_02930"
     CDS             complement(1860..>2582)
                     /locus_tag="VFU45_02930"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU5358050.1"
                     /translation="CSLTPARLGGEPARLAGILRYRISTEAAIVAISVEALLGWPMII
                     LGGAFLVWRYAPEWWQTAEPGIARHAHGMWPWFIVVAVVSVLVWWLVRRWMRVSAPTR
                     IQRSWQRMLVHWRRMPYWPIALSQPLSFVNVASRVAILPVLALTLPSPPPLGPLFLGS
                     FALLYSQMVLPTPSGAGAVELGFLAGAAGSLGDEGTALLFWWRFYTSAVGVILGLGLA
                     ARIYGWQAIRLWFLRRRPHPPA"
BASE COUNT          395 a          876 c          944 g          367 t
ORIGIN      
        1 cgtcgcagtt catctcgaac gtctggtgct ccgacgcggt catcacgtcc cgcaccgaga
       61 tgcgggaggc atcccgggcc aggtaatacc cgccccgtgc gccgcggacg ctctcgacca
      121 gcccggcccg ccgcaggcgc aggaggatct gttccacgta gtcgccgggg aggcgctccg
      181 cctccgcaag ttcacgtgcg gccaccggcg tgccgtcccc cgtggcccgc ttggccaggt
      241 ggagcgtgat gatgaggctg tactcggtcc aggtggtgac gcgcatggga cggaatatgg
      301 gaccatggcc ctagggattc aagaaccccc ggggggcccc ggtgccgggc tcacccgccg
      361 ccggggattc ccttcccgcc ggggacgccg agctcggtca tcgcgcggag gtcgagcacc
      421 tcctcgagtt cctttccctg gaggagcccc ttcttgacga ccagctgttt gacggtgagt
      481 ccggtggcga gcgcctcctt ggcgagcttg gccgactcgg cgtaaccgat cttcggcgcc
      541 agcgcggtga cgagcgccgg actccgctcc agccagtgct cgcactgggc cgcatccgcc
      601 tcgatgccca cgacgcaccg ctcggcgaac accgtcaccg cgttggtgag gatctgcatc
      661 gcaaagacga tgttgtgggt gatgacgggc atcatcacgt tgagctcgag ctgccccgcc
      721 tcggccgcga gcgccaccgt cgtgtcgagc ccgaggacct ggtagcagac ctggttgacc
      781 atctcggcga tcgacggatt gaccttcccc ggcatgatgc tcgacccggg ctgcaccgcc
      841 ggcaggagga tctcggccag gccggtgcgc ggacccgagg cgaggaggcg gatgtcgttg
      901 gcgatcttgt tgaggtcgag cacccaggcc cgcagggcgc cgctgaacga ggcgatgtcg
      961 cccatcgact gcatcagctg gatccggtcc tcggccacct cgaggtcgag cccggtggcg
     1021 gcgcgcaggt gcttgaccat cagcgccgga tactgcggtt cggcgttgat cccggtgccg
     1081 acggcggtgc cgccgatgtt catcgcgcgg agccagcggg cggcctcggc gagcttggcc
     1141 cgatggcgca gcacggtatg cccgtacgcc gtgaactcct ggccgagccg gataggcgtc
     1201 gcgtcctgga ggtgggtccg gccggacttg atgatgccgt cgaaggcctt ccccttcgcg
     1261 aggaacgccg cgccaagccc gtcgagcgcg gtcaggagcc gcggcaggct tgccaaggtg
     1321 gccaggcgca tcgcggtcgg gatcacgtcg ttggtgctct gcgccatgtt gacgtggtcg
     1381 ttggggtgca ccggcgcgta ggcgccgcgc ggcgcgccga ggagctcgtt ggcgcggttc
     1441 gcgagcacct cgttgcagtt catgttgtgg ctcgtgccgg cgccggcctg gtacacgtcg
     1501 acgatgaact ggtcgcggtg ctggccgccg agcacctcgt cggccgcgcg gacgatcgca
     1561 tcggccagcc gggcctcgag ccggcccgtc tccttgtggg tcagcgccgc ggccttcttg
     1621 atccacacca ccgcgtcgac gaacaacggc agcgggcgca ggccggagat ggggaagttc
     1681 tcgctggcgc ggagggtctg gatgccgtag agggcgtcgg cggggacggg cttttcgccg
     1741 agcgggtcct tctcgatgcg ggtcgccatg ggcgtcagtt cacggttcga ggtgcgcggg
     1801 gaccagcgcc ccgccgggcg gacgggaaaa aggtaacacc gccgcgggat gccggacccc
     1861 tacgccgggg gatggggccg acgacggagg aaccagaggc ggatggcctg ccaaccatag
     1921 atccgggcgg cgagcccgag gcccaggatc acgccgaccg cagaggtgta gaaccgccac
     1981 cagaagagga gtgcggtgcc ctcgtcgccg agcgaaccgg ccgcgcccgc caggaagccg
     2041 agctcgacgg caccggcgcc cgagggcgtg ggcagcacca tctggctgta gagcagcgcg
     2101 aacgagccaa ggaagagcgg ccccagtggc ggcggcgacg gcagcgtcag ggcgagcacg
     2161 ggaaggatgg ccacccgcga tgccacgttg acgaaggaga ggggctggct gagggcgatg
     2221 ggccagtacg gcatccggcg ccaatgcacc agcatccgct gccaggaccg ctggatcctg
     2281 gtcggcgccg acacccgcat ccaccggcgg acgagccacc agacgaggac gctcaccacc
     2341 gccaccacga tgaaccaggg ccacatcccg tgcgcgtggc gcgcgatccc gggttcggcg
     2401 gtctgccacc actccggggc gtagcgccac acgaggaacg cgccgcccag aatgatcatc
     2461 ggccagccaa gcagcgcctc gacgctgatg gcgacgatgg ccgcctcggt gctgatgcgg
     2521 tagcggagga tccccgcgag ccgcgccggt tccccgccca ggcgcgccgg ggtgagggaa
     2581 ca
//
LOCUS       DASUAP010000123         2883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1311756, whole genome shotgun sequence.
ACCESSION   DASUAP010000123 DASUAP010000000
VERSION     DASUAP010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311756"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..2701
                     /gene="alaS"
                     /locus_tag="VFU45_02935"
     CDS             89..2701
                     /gene="alaS"
                     /locus_tag="VFU45_02935"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU5358051.1"
                     /translation="MHSHVIRRKFLDFFVAKGHREVPSSSLVPADDPTLLFTNAGMNQ
                     FKRVFQGVEARDYRRATTVQKCVRAGGKHNDLEQVGFTKRHHTFFEMLGNFSFGDYFK
                     RDAITFAWEFVTSKEWLGLDPERLRVTVHHTDDEARGLWREVAGLPDERIYGLGDKDN
                     FWQMGDTGPCGPCTEIYVDLEWRPGQKAAHVAQAEFERLAEAGRFLEIWNLVFMQFDR
                     AADGTRTPLPRPSVDTGAGLERIAAVLQGEDSNFHTDLFLPLLHKAEQLVGKPYDRGP
                     AGAPFRVLADHARAVAFLLADGCYPSNEGRGYVLRRILRRGVRYAWLLGRKEPTLAPL
                     TAVVVQEMGHAYPELTTKAAYVADVTRQEEERFLETIEGGLGRMEELLAGGARVISGE
                     EAFKLYDTFGFPIDLTELIAAERGATVDVAGFEHALEGQRRRSRAVRTSQMGINAPAV
                     IAGQSGEWRKLREQKQAFVGYDTTGADTEILAFREAGGRVELLLRDNPFYVESGGQVS
                     DTGLVRGTGWTLAVDAVRKAPEGTVVSAAFEGSFEPTPVAALVDVPRRRNIERNHSAT
                     HLVHAALRRHLGDHVRQQGSLVEPGRLRFDFSHHGPIEPAVLSEIEREVNEQIWENRP
                     VTTREMATKDALALGAMAFFQDKYGDRVRVVSMDDYSIELCGGTHVRSTGQLGLFRFT
                     GQGGVAAGVRRIEAVTGPEAYNVVEALRGTIEEAAHAVKAQPDHLLHKLGQLVAERER
                     LEGRVAEFLKSGSGPGQGTTINVGEVAVTMAETSAEDRNEIAAVADTFRAGKAKAVLV
                     LLGSTDKGGVHVALTDDLVQGGRQARQLVERLGGKGGGRPYFASGSLARERQPDAAGL
                     PAVIASWLADGGKA"
     gene            2698..>2883
                     /locus_tag="VFU45_02940"
     CDS             2698..>2883
                     /locus_tag="VFU45_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358052.1"
                     /translation="MTHEAAWFESRTADAPAPLRARMRELVAHDWSADGLAAAAERGL
                     ADVIAHDGDRRVALDLLA"
BASE COUNT          431 a          993 c         1078 g          381 t
ORIGIN      
        1 caagcgggcg cccccacacc tcaagcaggc gacccccgca ccgagcgtct gagcgtctgt
       61 tgtccgttcc cccgctatat tcccggccat gcactcccac gtcatccgac ggaaattcct
      121 cgacttcttc gtcgccaaag ggcaccgcga ggtgccttct tcgtcgctcg tccccgccga
      181 tgacccgacg ctgctcttca ccaacgccgg gatgaaccag ttcaagcggg tgttccaggg
      241 ggtggaggcc cgcgactacc gccgggccac cacggtgcag aagtgcgtgc gcgccggcgg
      301 caagcacaac gacctggagc aggtgggctt caccaagcgg caccacacct tcttcgagat
      361 gctcggcaac ttctccttcg gcgattactt caagcgcgat gcgatcacct tcgcctggga
      421 gttcgtgacc tcgaaggagt ggctggggct cgacccggag cggctccgcg tcaccgtcca
      481 ccacaccgac gacgaggcgc gggggctctg gcgcgaggtg gccgggctcc ccgacgagcg
      541 gatctacggc ctgggcgaca aggacaactt ctggcagatg ggcgacaccg gcccctgcgg
      601 gccgtgcacc gagatctacg tggacctcga gtggcggccg ggccagaagg cggcccacgt
      661 ggcgcaggcg gagttcgagc gcctggcgga ggcggggcgt ttcctcgaga tctggaacct
      721 cgtcttcatg cagttcgacc gcgcggccga cggcacccgg acgccgctcc cgcgcccctc
      781 ggtcgacacc ggggcggggc tcgagcggat cgcggcggtg ctccagggcg aggactccaa
      841 cttccacacc gacctcttcc tgccgctcct ccacaaggcg gaacagctgg tcgggaagcc
      901 gtacgaccgc gggcccgccg gcgccccctt ccgggtgctg gccgaccacg cccgcgcggt
      961 ggccttcctc ctcgccgacg gctgctaccc gagcaacgaa ggccgcggct acgtgctccg
     1021 ccgcatcctc cgccgaggcg tgcgctacgc ctggctcctc ggccggaagg aaccgacgct
     1081 ggcgccgctc acggccgtgg tggtgcagga gatggggcac gcatatccgg aactcacgac
     1141 gaaggcggcc tacgtcgccg acgtgacccg gcaggaggag gagcgcttcc tcgagaccat
     1201 cgaagggggc ctgggccgga tggaggagct gctggcgggg ggtgccaggg tcatctcggg
     1261 cgaggaggcg ttcaagctct acgacacctt cggcttcccg atcgacctga ccgagctcat
     1321 cgccgccgag cgcggcgcca cggtcgacgt ggcggggttc gagcacgccc tcgagggcca
     1381 gcgccgccgc tcgcgcgcgg tacgtaccag ccagatgggg atcaacgcgc cggcggtgat
     1441 cgccggccag tccggggaat ggcggaagct ccgggagcag aagcaggcgt tcgtgggcta
     1501 tgacaccacc ggggccgaca ccgagatcct cgccttccgg gaggcggggg gccgggtgga
     1561 actcctgctc cgcgacaatc cgttctacgt cgagtcgggc gggcaggtga gcgacacggg
     1621 gctcgtgcgc ggcacggggt ggaccctcgc ggtcgacgcg gtccggaagg cgccggaggg
     1681 gacggtcgtc tcggccgcgt tcgaggggag cttcgagccg acgccggtgg cggcgctggt
     1741 cgacgtcccc cgccggcgca acatcgagcg gaaccacagc gccacccacc tggtgcacgc
     1801 ggcgctccgg cggcacctcg gcgaccacgt gcggcagcag ggctcgctgg tggagccggg
     1861 gcgcctccgc ttcgactttt cccatcacgg gccgatcgag ccggcggtgc tctcggagat
     1921 cgagcgcgag gtgaacgagc agatctggga gaaccgcccc gtcaccacgc gcgagatggc
     1981 caccaaggac gccctggcgc tcggcgcgat ggcgttcttc caggacaagt acggcgaccg
     2041 ggtccgcgtc gtgtcgatgg acgactactc catcgagctc tgcggcggca cccacgtgcg
     2101 ctcgaccggc cagctcgggc tcttccgctt cacggggcag ggaggcgtcg cggcgggggt
     2161 gcgacggatc gaggcggtga cgggccccga ggcgtacaac gtggtcgagg cgctgcgggg
     2221 gacgatcgag gaggccgcgc acgcggtgaa ggcccagccg gaccacctcc tccacaagct
     2281 cggccagctg gtggcggagc gggagcggct cgaagggcgg gtggcggagt tcctcaagtc
     2341 cggcagcggc ccgggccagg gcaccacgat caacgtgggc gaggtggcgg tgacgatggc
     2401 ggagacctcg gccgaggacc ggaacgagat cgccgccgtg gccgacacct tccgcgccgg
     2461 gaaggcgaag gcggtgctgg tgctcctcgg ctcgaccgac aaggggggcg tgcacgtggc
     2521 gctcaccgac gacctggtcc aggggggccg ccaggctcgg cagctggtgg agcggctggg
     2581 cgggaagggt ggcggacggc catatttcgc gtcgggcagc ctggcgcggg agcggcaacc
     2641 cgacgccgcc ggattgccgg cggtgatcgc gtcctggctc gcagacggag gtaaggcgtg
     2701 acccacgaag cggcctggtt cgaatcgcgc acggccgacg cgccggcgcc cctccgggcg
     2761 cggatgcggg agctggtggc gcacgactgg tcggccgacg ggctggcggc cgcggcggag
     2821 cgggggctcg cggacgtcat cgcgcatgac ggcgaccggc gcgtggcgct cgacctcctg
     2881 gcc
//
LOCUS       DASUAP010000124         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1189089, whole genome shotgun sequence.
ACCESSION   DASUAP010000124 DASUAP010000000
VERSION     DASUAP010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189089"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..949)
                     /locus_tag="VFU45_02945"
     CDS             complement(152..949)
                     /locus_tag="VFU45_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU5358053.1"
                     /translation="MPAIVATASVAPVLKEPNIRSEQITQLVLGRTGRVVEDRGEWHR
                     IHVDLDGYEGWCHAGYSRVLDDEAAAHWIASADGWSEGATVEQGDRMVRVPLGGRVRL
                     GRHGAELPDGTIGRILGGRIVPAATIATEARSTPVDKWLVKFYGGAPYQWGGLTPWGT
                     DCSGLVQVGFAARGIALPRDSFLQAETGTEVDLASARRGDLLFFSENGRSVTHVAVLG
                     PDDRLVHSSLSAGGFIVEAWTAGTRAGFLRDLFVTARRPPAPEGAAR"
     gene            complement(986..1165)
                     /locus_tag="VFU45_02950"
     CDS             complement(986..1165)
                     /locus_tag="VFU45_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358054.1"
                     /translation="MPEIKEHDAMRCRACGNEERASEGYPCIDCGTFVCLICHFKGIE
                     RCAECEKKLKEGAGK"
     gene            complement(1179..2528)
                     /locus_tag="VFU45_02955"
     CDS             complement(1179..2528)
                     /locus_tag="VFU45_02955"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5358055.1"
                     /translation="MADTVLLIDDDPEILRAIGNYFEHVGYEVGRELTGEAGLEAYER
                     LRPEVVVLDLQLPGMDGLEVLERLREKHAAVILLTGNSDIPTAVKAMQLGAENFLTKP
                     VDMPHLVAATAKVADKVRLRRVNELLLASVVQGEGLDSLGQSGPMQDFARQVGLLAQS
                     DRTTVLLVGESGTGKGWVARMIHNLSPRAKAPFVEVNCGGLSATFLDSELFGHEKGAF
                     TDAKDRKQGLFEIADRGTIFLDEIGELAIELQPKLLKVLETKTFRRLGGTREITVDVR
                     LIAATNRDLPADVAAGRFREDLFYRLNVMPLELPPLRTRSREDRLALITRLFQDLRSH
                     VPGAPSLIAPDALERLLAHPWPGNVREMRNVLERALILGRGQVAVAAEHLPADFKSRS
                     GSAGDRRHAPLSLDDLERQHIERTLRHHAGNRTRAAEELGISRATLINKIKRYTITI"
     gene            complement(2533..>2860)
                     /locus_tag="VFU45_02960"
     CDS             complement(2533..>2860)
                     /locus_tag="VFU45_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5358056.1"
                     /translation="VVLNLVSNAEHAMAGVDSATGIIRTWADARSVGCEVLDPGAGIP
                     PEVIGRIFEPFFTTKPAGQGTGLGLSISHGIIRAHRGEIRAQNRPEGGARLWFELPRG
                     GAAAPR"
BASE COUNT          380 a          972 c         1084 g          424 t
ORIGIN      
        1 atctgcgggt cggtcttgcc gtcgaaccgc acgcgcgcgc cagccgccgc cgagtccacc
       61 tcgtcggcga cggcccggag gatggcccgg cgaccggcgc ccgccgtgag gaggagcgtg
      121 ccgtcgatgt cgaagaggac gagggtgtgg ttcaccgggc ggcgccctcg ggggccgggg
      181 gccggcgcgc ggtgacgaag aggtcgcgca ggaaccccgc ccgcgtgccc gctgtccacg
      241 cctccacgat gaacccgccg gcggacaggc tcgagtggac cagccggtcg tcggggccga
      301 gcaccgccac gtgggtcacc gagcggccgt tctccgagaa gaagagcagg tcgccgcggc
      361 gcgccgacgc gaggtcgacc tccgtgccgg tctcggcctg gaggaacgag tcgcgcggga
      421 gcgcgatgcc gcgggcggca aagccgacct gcaccagccc cgagcagtcg gtgccccagg
      481 gggtgagccc gccccactgg tacggcgcgc cgccgtagaa cttgacgagc cacttgtcga
      541 ccggggtgct gcgcgcctcg gtggcgatgg tcgccgccgg tacgatcctg ccgccgagga
      601 tccggccgat ggtcccatcg ggaagctcgg cgccgtgccg gccgagccgg acgcggccgc
      661 ccagggggac gcggaccatc cggtccccct gctcgacggt ggccccctcg ctccagccgt
      721 cggcggaggc gatccagtgc gcagcggcct cgtcatcgag gacgcggctg tagccggcgt
      781 ggcaccaccc ctcatagccg tcgagatcga cgtggatccg gtgccactcg ccccggtcct
      841 cgaccacgcg gcccgtccgg ccgaggacga gctgggtgat ctgctccgaa cggatgttcg
      901 gctccttgag gactggcgcg acggatgcgg tggcgacgat ggctggcatg gggtccccgg
      961 gcggcgcgga ggaggggcgg gccggctact tgccggcgcc ttccttcagc ttcttctcgc
     1021 attcggcgca gcgttcgatg cccttgaagt ggcagatcag gcagacgaac gtgccgcaat
     1081 ctatgcaggg atatccctcc gatgcccgtt cctcgttgcc gcaggcgcgg caacgcatcg
     1141 catcgtgttc cttgatctcg ggcacggagc ctccgccgtc agatggtgat cgtgtaccgc
     1201 ttgatcttgt tgatgagcgt cgcgcgggag atgccgagct cctcggcggc gcgggtccga
     1261 ttgccggcgt ggtgccggag cgtgcgctcg atgtgctggc gctcgaggtc gtcgaggctc
     1321 agcggcgcgt ggcggcggtc gccggcggag ccgctgcggc tcttgaagtc ggccggcagg
     1381 tgctcggcgg cgacggccac ctggccgcgg cccaggatga gcgcgcgttc gaggacgttg
     1441 cgcatctcgc gcacgttccc gggccagggg tgggccagga gccgctcgag cgcgtcggga
     1501 gcgatgaggc tgggcgcgcc cggcacgtgg ctgcggaggt cctggaagag ccgggtgatg
     1561 agggccagcc ggtcctcgcg cgagcgggtg cggaggggcg ggagctcgag cggcatcacg
     1621 ttgaggcggt agaacaggtc ctcccggaag cgcccggcgg ccacgtcggc ggggaggtcg
     1681 cggttggtgg cggcgatcag gcgcacgtcg acggtgatct cgcgggtgcc gcccagccgg
     1741 cggaaggtct tggtctcgag caccttgagg agcttgggct ggagctcgat ggcgagctcg
     1801 ccgatctcat cgaggaagat cgtgccgcgg tcggcgatct cgaagaggcc ctgcttgcgg
     1861 tccttggcgt cggtgaaggc gcccttctcg tgcccgaaga gctcggagtc gaggaacgtg
     1921 gcggagaggc cgccgcagtt gacctccacg aacggcgcct tggcgcgggg gctcaggttg
     1981 tggatcatcc gcgccaccca gcccttgccg gtgccgctct cgcccaccag cagcacggtg
     2041 gtccggtcgc tctgtgccag caggccgacc tgccgggcga agtcctgcat cgggccggac
     2101 tggcccagcg agtcgagccc ctcgccctgc accacgctgg ccagcagcag ctcgttgacc
     2161 cggcggaggc ggaccttgtc ggccaccttg gccgtggccg cgacgaggtg cggcatgtcg
     2221 accggcttgg tgaggaagtt ctccgcgccg agctgcatcg ccttgacggc ggtggggatg
     2281 tcgctgttgc cggtgagcag gatgaccgcc gcgtgcttct cgcgcaggcg ctcgagcacc
     2341 tcgagaccgt ccatccccgg cagctgcagg tcgaggacga cgacctcggg ccggagccgc
     2401 tcgtacgcct ccaggccggc ctcgccggtc agctcccggc ccacttcgta gccgacgtgc
     2461 tcgaagtagt tgccgatcgc ccggaggatc tcgggatcgt cgtcgatcag gaggacggtg
     2521 tccgccatcg catcacctcg gtgccgcggc gccgccgcgg gggagttcga accagagacg
     2581 ggcgccgccc tcggggcggt tctgggcgcg gatctcgccc cggtgtgccc ggatgatacc
     2641 gtgggatatg gaaaggccaa gcccggtgcc ctgccccgcc ggcttggtgg tgaagaacgg
     2701 ttcgaagatc cggccgatga cctccggggg gatcccggcg ccgggatcga gcacttcgca
     2761 ccccacggac cgcgcgtccg cccaggtccg gatgatcccc gtcgccgagt cgacccctgc
     2821 catcgcgtgc tcggcgttgg agacgaggtt gagcaccacc
//
LOCUS       DASUAP010000125         6150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_671083, whole genome shotgun sequence.
ACCESSION   DASUAP010000125 DASUAP010000000
VERSION     DASUAP010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671083"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFU45_02965"
     CDS             <1..274
                     /locus_tag="VFU45_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358057.1"
                     /translation="YQRLVPVRATLRVRSDRDTFDGSVTITPGVVRISGPVAAVKRVD
                     GVPTVPFDLPPGADPAGLTVALDTARLPGLRVTPGDVTIRVRPPTP"
     gene            271..1314
                     /gene="tsaD"
                     /locus_tag="VFU45_02970"
     CDS             271..1314
                     /gene="tsaD"
                     /locus_tag="VFU45_02970"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU5358058.1"
                     /translation="MTSILAIETSCDETSAAVVTAGPDGRPALRSLVILSQDIHKVFG
                     GVVPELASRAHLAALPAVVDQALVEAGLSRERVDAIAVTQGPGLVGALLVGAMYAKTL
                     AWALHKPLLGVHHLEGHLFAATLEDPAFEPPFVGLLVSGGHTMLLDVPAWGTYRLLGQ
                     TRDDAAGEAFDKVAILLGLGYPGGPRIERLAREAKAGTFKLPRPMLGELDRPGPDRYS
                     FSFSGLKTAVLRASQQSDDLERDKPRLARDFQDAAIDVLTEKTAHAVAELGYPTAMIG
                     GGVACNQALIAALRQKLGTTARVVAATPRLNTDNAAMIGAAAAWRLARGERSGFDLEP
                     RADLAFPGLVGGR"
     gene            1443..2300
                     /locus_tag="VFU45_02975"
     CDS             1443..2300
                     /locus_tag="VFU45_02975"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU5358059.1"
                     /translation="MTVYPFVLHLGSFEITGYGIMMMVAFLAGSWLIALELRRRGFRE
                     EYASDVTLAALVGGIIGAKLWYVALTGDPATLFSRGGLVWYGGFIGGALGVVLNGWRL
                     RVPFRWTAQLTAPALAAAYALGRVGCFVVNDDYGRPTSLPWAMKFPQGLPPSTAGNMT
                     QLFGIPVPPGTDPSTVLAVHPTQLYEIAAMLVVFAILWRWRTLPRGTGWLFGAYLVFA
                     GVERFLVEIVRAKDDRFVGPFTLAQVTSVLLVIVGVVAMRKLRDAGQLAPGGYLQPTA
                     GADAKPTEA"
     gene            complement(2710..4830)
                     /locus_tag="VFU45_02980"
     CDS             complement(2710..4830)
                     /locus_tag="VFU45_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c/FTR1 family iron permease"
                     /protein_id="HEU5358060.1"
                     /translation="MLALFAVIFPLTAARAERVDDVTATVRRVSSIAVLAAQEYRTGF
                     SGGKLVSPKEQDEAKLFLSEARRSAALLPPPGGPAAVRAIDSLVALVAAAAPPDTVSA
                     RAQALGDQLAAAFSVTLDDIPAEAPSLAHGADLFAHDCATCHGMAGRGDGPSAAGLSP
                     RPADLTSLPTLADRSPLDFYRRISLGVPGTAMPAYEAQLAPADRWALAVYASTLRLPS
                     PAGTAPPSLRTFPATAVMTDRAVVAVLAPAGDLFDPSVAARVAAVRRFGTDQRSGLAA
                     GPVFDKVRAQLAQGIELARQGKAAEAGAVALDAYMTFEAVEGAVSSKDGALGSRLESA
                     FAAFRARAAGGAGPDELARLHSSLLAGLEEAERTVADVPSPLNLFVQSLALLLREGLE
                     AILIVGALIAFLLKTGAKQRTRDVHIGVAAAIAASLLTAVALETIFEVSSARREALEG
                     ATMVVATGVLFYVSYWLLSKMEVAKWNRFVKAQVQDAVTGGSALALASVAFLAVYREG
                     FETVLFYKALFLSGGSGNTVFPVVAGMVVGAVGLTIIYIAINRFGVRIPLKPFFAITS
                     AFLYYMAFVFAGKGIAELQGAGVVSLTPFSPEIRFPTLGIYSTWETLSVQAVLVLLLL
                     GAVVWTFLVEPRRLRVTGVMVPEPAAPAAPRAPVTAVRSALDPAVGRSLERMEADLGE
                     LRAELERLKDRLVQDGVDRLTKGD"
     gene            complement(5171..5557)
                     /gene="acpS"
                     /locus_tag="VFU45_02985"
     CDS             complement(5171..5557)
                     /gene="acpS"
                     /locus_tag="VFU45_02985"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU5358061.1"
                     /translation="MNVLGVGIDLMDIARVRAMLERREEQAITKLLTDDERAFVMAQP
                     DPARHLAARIAAKEAVYKALQALPGTRGVGWHDLEVLRSVDGRPSIRLLGVAARIDRE
                     QGPLVIELSLSHSDVTAGAVAVVGKA"
     gene            complement(5554..>6150)
                     /locus_tag="VFU45_02990"
     CDS             complement(5554..>6150)
                     /locus_tag="VFU45_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358062.1"
                     /translation="DLVTRLLELAGAARADLERGETTASVRRLAPDAWRARWESAAAA
                     AAAVVRTRIDEEMVRAAGRSGFPLRRLLRLAVSDDEARSIELRLSACAAPLAVALEGV
                     ARASAGAGWPDAMVRAAQALEVSWLELESVAVAEEHAWRPEVDRIAHWRPARWPRWVA
                     AAAVLLVTGYAGLVLGGYLPVPAPLRPAARVWWDKVEP"
BASE COUNT          957 a         2199 c         2100 g          894 t
ORIGIN      
        1 ctaccagcgg ctggtgccgg tccgcgccac cctgcgggtc cggagcgacc gcgacacctt
       61 cgacggcagc gtcacgatca cgccgggtgt ggtgcggatc tcgggcccgg tggcagcggt
      121 caagcgggtc gacggcgtgc ccaccgtccc cttcgacctg ccgcccggtg ccgacccggc
      181 ggggctcacc gtggcgctcg acaccgcgcg ccttccgggt ctgcgcgtga cgccgggcga
      241 cgtgaccatc cgcgtccgtc caccgacacc atgaccagca tcctcgcgat cgaaacgtcc
      301 tgcgacgaaa cctccgctgc ggtggtcacg gcggggcccg atggccggcc ggcgctccgg
      361 agcctcgtca tcctctcgca ggacatccac aaggtcttcg ggggcgtggt gcccgagctc
      421 gcgagccggg cgcaccttgc cgccctgccc gcggtggtcg accaggcgct ggtcgaagct
      481 gggctgtccc gcgagcgggt cgacgccatc gccgtgaccc agggccccgg cctggtcggc
      541 gcactcctcg tcggcgcgat gtacgccaag accctcgcct gggcgctgca caagccgctc
      601 ctcggcgtgc atcacctcga agggcacctc tttgccgcga cgctcgaaga tcccgcattc
      661 gagccgccct tcgtggggct cctggtcagc ggcgggcaca ccatgctgct cgacgtcccc
      721 gcctggggca cgtaccgact gctgggccag acccgcgacg acgccgccgg cgaggcattc
      781 gacaaggtgg ccatcctcct cgggctcgga tatcccggcg ggccgaggat cgaacggctc
      841 gcacgcgagg ccaaggccgg aacgttcaag cttccccgcc cgatgctcgg cgagctcgac
      901 cggccgggcc cggaccgcta ttcattctcc ttcagcgggc tcaagacggc ggtgctccgg
      961 gccagccagc aatcggatga cctcgagcgc gacaagccca ggctggcgcg ggacttccag
     1021 gatgccgcca tcgacgtcct cacggagaag accgcccacg ccgtggcgga actgggctac
     1081 cccaccgcga tgatcggcgg cggtgtcgcg tgcaaccagg cgctcatcgc cgcgctccgc
     1141 cagaagctcg gcaccaccgc gcgcgtggtg gccgcgaccc cccgcctcaa caccgacaat
     1201 gccgcgatga tcggcgcagc ggcggcgtgg cgcctggccc gcggggagcg gagcggattc
     1261 gacctcgaac cgcgcgccga cctggcgttc ccggggttgg tgggtggtcg ttagtcgttg
     1321 gtcgttagtc gttggtcgtg agtgccagaa atgagtgccg agtgcgcatc agcttgtggc
     1381 tgatagctga tagccgatag ctgatagctt atggctgata gcccttccac ccggatcccc
     1441 gcatgaccgt ctatccgttc gtcctccatc tcgggtcgtt cgagatcacc ggctacggca
     1501 tcatgatgat ggtggcgttc ctggccggga gctggctcat cgcgctcgag ctccggcgcc
     1561 ggggcttccg ggaggagtac gcgtccgacg tgacgctggc ggcgctggtg ggggggatca
     1621 tcggggccaa gctctggtat gtggccctga ccggcgaccc ggccacgctc ttttcccggg
     1681 gcgggctggt ctggtacggc ggcttcatcg gcggcgccct cggcgtggtg ctcaacgggt
     1741 ggcggctccg ggtgccgttc cgctggaccg cccagctcac cgcccccgcg ctcgccgccg
     1801 cctacgccct cggacgggtc gggtgcttcg tcgtcaacga cgactacgga cggccgacgt
     1861 cgctcccctg ggccatgaag ttcccgcagg ggctgccgcc atcgacggcc gggaacatga
     1921 cccagctctt cgggatcccg gtgccgcccg gcaccgaccc gagcacggtg ctcgccgtcc
     1981 atccgaccca gctctacgag atcgccgcga tgctcgtggt cttcgccatc ctgtggcgct
     2041 ggcggacgct gccgcgcggc accggctggc tcttcggcgc ctatctcgtc ttcgccgggg
     2101 tcgagcgctt cctggtcgag atcgtgcgtg ccaaggacga ccgcttcgtg gggccgttca
     2161 ccctggcaca ggtgaccagc gtactgctcg tcatcgtggg ggtggtggca atgcgcaagt
     2221 tgagggatgc cgggcagctc gccccgggcg gataccttca acccaccgca ggcgccgacg
     2281 ccaagcccac agaagcttga cacatcgaac ttctgtcaaa tttctaggca attacgtaag
     2341 tcgttgtggt acaatgtatt aagcaataac tcttgtctgc tgtaaaattt ctgacacctg
     2401 aagcccacct cagcggaccg gcgctgccga cgtaactctt tgcggttgaa agtattacaa
     2461 atttgcagac gactggcacc ggagttgccc tagtagtggg cgaagggtcg cactggcccc
     2521 acggaaaagt ggcaggtaga agaagggttg gagcgggcac ttccgtccgt ctccgtatag
     2581 ctcagggggc tacctgtcac ctccttagaa acggtccacc tcggtgggcc gtttcgcttt
     2641 tccataccca cgaagaaggc cccggtagag acgccggggc ctttctcgtg attcaggatc
     2701 gtgaacggct cagtcgccct tggtgagccg atccacgcca tcctgcacca gccggtcctt
     2761 gagccgctca agctctgccc gcagctcccc caggtcggcc tccatccgct ccagcgagcg
     2821 ccccacggcc ggatcgagcg ccgaccggac ggccgtcacc ggcgctcttg gcgcagccgg
     2881 ggccgccggc tccggcacca tgaccccggt gaccctgagt cgacgcggct cgacaaggaa
     2941 cgtccatacc acagcgccga ggagcagcag caccagcacc gcctggaccg acagcgtctc
     3001 ccaggtggag tagatgccga gtgtggggaa ccggatctcg gggctgaagg gagtgagcga
     3061 gaccacgccg gccccctgca gctcggcgat ccccttcccg gcaaagacga acgccatgta
     3121 gtagaggaac gcgctcgtga tggcaaagaa cggcttgagc gggatccgga cgccgaaccg
     3181 gttgatcgcg atgtaaatga tcgtgagccc gaccgcgccg accaccatcc cggccacgac
     3241 ggggaacacg gtgttgccgc tgccccccga gaggaagagc gccttgtaga agagcaccgt
     3301 ctcgaaccct tcgcggtaga ccgccaggaa cgccaccgac gcgagcgcga gcgcggagcc
     3361 cccggtcacc gcgtcctgca cctgggcctt cacgaaccgg ttccacttgg ccacttccat
     3421 cttcgagagc agccagtagc tcacgtagaa caggaccccg gtggccacca ccatcgtggc
     3481 gccctcgagc gcctcccgcc gggccgaact cacctcgaag atcgtctcca gcgccacggc
     3541 ggtcaggaga ctcgcagcga tcgccgcggc gacgccgatg tggacatcgc gggtgcgctg
     3601 cttggcgccg gtcttgagca ggaaggcgat gagcgcgccg acgatcagga tggcctcgag
     3661 cccctctcgc aggaggagcg ccagcgactg gacgaacagg ttgagcggcg aggggacgtc
     3721 ggccacggtc cgttcggcct cctccagacc ggcgaggagc gagctgtgca gccgcgccag
     3781 ctcgtcgggg cccgccccgc ccgccgcccg ggcacggaac gccgcaaatg ccgactccag
     3841 ccgggatccg agggcaccgt ccttcgagct gaccgcgccc tcgaccgcct cgaacgtcat
     3901 gtacgcatcg agcgccaccg cgccggcttc ggccgccttc ccttgccgcg caagctcgat
     3961 gccctgggcc agctgggcgc gcaccttgtc gaacacgggg ccggccgcca gtcccgagcg
     4021 ctggtcggtg ccgaaccggc gcaccgccgc gactcgcgcg gcgaccgatg ggtcgaacag
     4081 gtcgccggcc ggggcgagca ccgccacgac ggcgcggtcg gtcatgacgg cggtggccgg
     4141 gaaggtccga agcgacgggg gagccgtgcc cgcgggggat ggaaggcgca gggtggaggc
     4201 gtagaccgcg agcgcccagc ggtcggccgg ggcgagctgc gcttcgtacg ccggcatcgc
     4261 ggtgcccggc acgccgaggg agatgcgacg gtagaagtcg aggggcgagc gatcggccag
     4321 ggtcggcaac gaggtcaggt cggcggggcg gggcgagagt cccgccgccg acggcccgtc
     4381 accccggccc gccatcccgt ggcaagtggc gcagtcgtgc gcgaagagat cggcaccgtg
     4441 ggccagcgag ggtgcctcgg cggggatgtc gtcgagcgtc acgctgaagg cggcggcgag
     4501 ctggtcgccc agggcctggg cccgcgcgga caccgtgtcc ggaggcgccg ccgcggcgac
     4561 gagggccacc agggaatcga tggcgcgcac cgccgccggc ccgcccggcg gggggaggag
     4621 cgccgcactc cggcgtgcct cgctcaggaa gagcttggcc tcgtcctgct ccttgggcga
     4681 gacgagcttc ccgccggaga atccggtgcg atactcctgc gccgcaagca ccgcgatcga
     4741 cgacacccgt cgcaccgtcg cggtgacgtc atctacccgc tcggcccgtg cagcggtgag
     4801 tgggaagatc acggcaaaga gtgcgagcac cgtggcgcgt ttgataatca ttatcaaccg
     4861 attccgtggc ggaccaaaca aagtagagcg agttgctagg aattggaaga tggggactta
     4921 ttgggctgcg agacgcgagg ggcgaggcac acgggggcca cgggcgacga ggagcgggac
     4981 cggaaccgat ggaagggaca ccgctgggga gcacagtcca aaaggatccg agaacgtgac
     5041 cgggtcattc ccggcacgct ccgaacccgt ctccactttc gatgccttct ggcagcccgc
     5101 tcacctctta cctcttacct cttacctctt acctctcacc ccttacccct cacctctcac
     5161 ctctcacccc tcacgccttc cccaccaccg ccaccgcccc cgcggtcacg tcggaatggc
     5221 tgagggagag ctcgatgacc aggggaccct gctcgcggtc gatccgggct gcgacgccca
     5281 ggagcctgat cgacggccgc ccgtcgacgc tgcggagcac ctcgaggtcg tgccagccga
     5341 cgccgcgggt cccggggagc gcctgcagcg ccttgtagac cgcctccttg gcggcgatcc
     5401 gcgccgcgag gtggcgcgcg ggatcgggct gggccatcac gaatgcgcgc tcgtcgtcgg
     5461 tgaggagctt ggtgatggcc tgctcctccc gccgttccag catcgcgcgc acccgcgcga
     5521 tgtccatcag gtcgatcccg acgccgagga cgttcacggc tccaccttgt cccaccagac
     5581 ccgggccgcg gggcggagcg gcgccgggac cgggaggtat ccgccgagga cgagccccgc
     5641 atatcccgtc acgaggagca ccgccgccgc ggccacccac ctgggccagc gggcgggacg
     5701 ccagtgtgcg atgcgatcca cctcggggcg ccaggcgtgc tcttccgcga cggccaccga
     5761 ctcgagctcg agccacgaca cctcgagcgc ctgcgccgcc cggaccatcg catcgggcca
     5821 ccccgcccct gcggatgcgc gggcgacgcc ctcgagggcc accgcgagcg gcgcggcgca
     5881 ggcggacagg cggagctcga tcgaacgtgc ctcatcatcc gacaccgcca gtcgaaggag
     5941 gcgccggagc gggaatcccg agcgtcctgc cgcgcggacc atctcctcgt cgatccgcgt
     6001 acgcaccacc gcggcagcag ccgcggcggc ggattcccag cgcgctcgcc acgcgtccgg
     6061 ggccagccgc cggacggacg ccgtggtctc gccccgttcg aggtcggcgc gcgcggcgcc
     6121 ggcgagttcg aggagcctgg tcacgaggtc
//
LOCUS       DASUAP010000126         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_834743, whole genome shotgun sequence.
ACCESSION   DASUAP010000126 DASUAP010000000
VERSION     DASUAP010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834743"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFU45_02995"
     CDS             complement(<1..200)
                     /locus_tag="VFU45_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358063.1"
                     /translation="MIKRLAFLAALAAAVPAALAAQQPAVDTTGVGAVVTQATDHSQV
                     MAILQHLSDAIGPRLTGAPAVK"
     gene            complement(227..1018)
                     /locus_tag="VFU45_03000"
     CDS             complement(227..1018)
                     /locus_tag="VFU45_03000"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU5358064.1"
                     /translation="MPRPEPARADRARGALLGLALGGDATAPLALLLAEELLAPEVDL
                     RRLADRWIGWVRRDGRGVDDWTRTALEHIARHDSPPSAMPAPVRAAGLGRAIPVALAA
                     SRSPRNLVSGTWHVVALTHPDPRCTWGAVAVNVAVACFLDGRRDIVPDVVEALRNNDA
                     PAELLAAVRRVPLERWEEIGRNGDDAPPVVLDVERALWCAHHAPDLDVAADLLACDGD
                     RDALPLVGALLGARDGIEGRGAGRLASVPAAAQLRSAADRLIGAA"
     gene            1108..2394
                     /locus_tag="VFU45_03005"
     CDS             1108..2394
                     /locus_tag="VFU45_03005"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEU5358065.1"
                     /translation="MSKDTLSITDNRTGKTYELPVSDGTIKAVDLRQIRTSDDDFGLM
                     TYDPAFMNTAACRSAITYIDGDKGILRYRGYPIEQLAAEASFLEVAYLLNEGELPNKQ
                     QLDAWVRDITYHTYVHTNIIKFLEGFRYDAHPMGVLLGAVGALSTFYPDAKHIEDPKN
                     RYIQRVRLLAKMPTLAAFAFRHSRGLPYEFPRNDLDYIGNFVNMTFSIGGHHQPNPVL
                     EKALEILLILHADHEQNCSTSAVRAVGSSHVDPFSAVTAGIAALYGPLHGGANEAVLL
                     MLDEIGDKKNIPAFIDKVKSGTGRLMGFGHRVYKSYDPRAKLIKKVADDVFAQTGLNP
                     KLEIALELERIALSDEYFIKRKLYPNVDFYSGLIYQAMGYPTDYFTVLFAMGRTPGWL
                     AQWEEMLLDKEQKIARPRQVYTGPAERPFVPMAKRK"
BASE COUNT          436 a          951 c          885 g          355 t
ORIGIN      
        1 tgcttgaccg ccggggcgcc ggtgagccgg gggccgatgg catcgctcag gtgctgcagg
       61 atggccatca cctgggaatg gtcggtcgcc tgggtcacga ccgcgccgac gccggtggtg
      121 tcgacggcgg gctgctgggc cgccagggcg gccggtaccg cggccgcgag ggccgccagg
      181 aatgcgagac gcttgatcat cgttgcatcg ctcggtgaga ggggactcag gcggccccga
      241 tcaggcggtc cgccgcggaa cgaagttgcg ccgctgccgg caccgacgcc agccggccgg
      301 ccccccggcc ctcgatgccg tcccgggcgc cgaggagcgc gccgacgagc gggagcgcgt
      361 cacgatcgcc gtcgcacgcc aggaggtccg ccgccacatc gaggtcggga gcgtggtgcg
      421 cgcaccagag ggcgcgctcc acgtccagca ctacgggcgg ggcgtcgtcc ccgttgcggc
      481 cgatctcctc ccagcgctcc aggggcaccc gccggaccgc ggcgaggagt tcggccggcg
      541 cgtcgttgtt gcggagcgcc tcgaccacgt cggggacgat gtcgcgccgg ccgtccagga
      601 agcacgccac ggccacgttg accgccaccg ccccccaggt gcaccggggg tcgggatggg
      661 tgagcgccac cacgtgccac gtgccgctca cgaggttccg cggcgaccgg gacgccgcca
      721 gcgccaccgg gatcgcgcgc ccgagccccg ccgcccggac cggcgccggc atggccgacg
      781 gcggggagtc gtgccgggcg atgtgctcga gcgccgtccg ggtccagtcg tccaccccgc
      841 ggccgtcccg ccgcacccag ccgatccagc ggtcggcgag ccgccgcagg tccacctccg
      901 gagcgagcag ctcctcggcc aggaggagcg cgagcggcgc ggtcgcgtcg cccccgagcg
      961 ccagcccgag gagcgcgccg cgggcgcggt ccgcgcgcgc gggttcaggc cggggcaccc
     1021 atgcccccgg cgtaccatcc gccggacagc ggactatgtt tagatgctgg caaggtcaat
     1081 ctccgcccaa gctcgaggac agcgctcgtg tccaaagaca ctctctccat caccgacaac
     1141 cgcaccggca agacgtacga actcccggtc tccgacggca ccatcaaggc ggtggacctg
     1201 cggcagatcc gcacttcgga tgacgacttc gggctgatga cgtacgaccc ggcgttcatg
     1261 aacaccgccg catgccggag cgccatcacc tacatcgacg gcgacaaggg gatcctgcgc
     1321 taccgcggct atcccatcga gcagctcgcc gcggaggcga gcttcctcga ggtggcgtac
     1381 ctcctcaacg agggggagct ccccaacaag cagcagctcg acgcctgggt ccgtgacatc
     1441 acctaccaca cgtacgtcca caccaacatc atcaagttcc tcgagggctt ccgctacgac
     1501 gcccacccga tgggcgtcct cctgggcgcg gtcggggccc tgtcgacatt ctaccccgat
     1561 gcgaagcata tcgaggaccc gaagaatcgc tacatccagc gcgtgcggct cctggccaag
     1621 atgcccaccc tggcggcgtt cgcgttccgg cactcccgcg ggctgcccta cgagttcccg
     1681 cggaacgacc tcgattacat cggcaatttc gtcaacatga cgttcagcat cggcggccac
     1741 caccagccca acccggtgct ggaaaaggcc ctcgagatcc tgctcatcct gcacgccgac
     1801 cacgagcaga actgctccac cagcgccgtc cgcgcggtcg gatcgagcca cgtcgacccg
     1861 ttctcggccg tgacggcggg gatcgcggcg ctctacggcc cgctccacgg cggcgccaac
     1921 gaggccgtgc tcctgatgct cgacgagatc ggcgacaaga agaacatccc ggcgttcatc
     1981 gacaaggtga agtccgggac cggccggctg atggggttcg gccaccgggt ctacaagtcc
     2041 tacgacccgc gggccaagct catcaagaag gtggccgacg acgtcttcgc ccagaccggg
     2101 ctcaacccga agctcgagat cgcgctcgag ctggagcgga tcgcgctgtc ggacgagtac
     2161 ttcatcaagc ggaagctcta ccccaacgtc gacttctatt ccgggctgat ctaccaggcg
     2221 atgggctacc cgaccgacta cttcaccgtg ctcttcgcga tggggcggac gccggggtgg
     2281 ctggcccagt gggaggagat gctgctcgac aaggaacaga agatcgcccg gccgaggcag
     2341 gtctacaccg gccccgccga gcgccccttc gtaccgatgg ccaagcggaa gtgagacgcg
     2401 gtgagcggta agaggtgagc ggtgagcggt gagcggtgag cggggagatg tgagggtcag
     2461 tagtgtgaag tggtagggtg aggggaccca tcctggatcc ccgagacgag cgaccctccg
     2521 gaccagtcga gccccggagg gtcgctcaca ttcgggatgc acgccggcta ccctctcccc
     2581 gctaccctcc caccgcgcac cacataccgc tccccattgg tctcccg
//
LOCUS       DASUAP010000127         6813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1189152, whole genome shotgun sequence.
ACCESSION   DASUAP010000127 DASUAP010000000
VERSION     DASUAP010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189152"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFU45_03010"
     CDS             <1..519
                     /locus_tag="VFU45_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU5358066.1"
                     /translation="WRKVDLTRDAAGARVRLNDRDMHVDLGGIAKGYGIDRAVAAIRR
                     LGIAHAIVTVGGDLYALGPSPSGEPWEVGIRDPHHLDQLAGRLEVSDRAVTTSGDYER
                     FFEYHGVRYHHLIDPVTAAPRRTGVHSVTVLGADCFNADAASTAAFGLDRDTARRMVR
                     RVLSDAEVIPLT"
     gene            533..886
                     /locus_tag="VFU45_03015"
     CDS             533..886
                     /locus_tag="VFU45_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358067.1"
                     /translation="MKSHQLYCGACDRPVRVLITEQALGETQAPVRDAEFICLEIGTT
                     CTGRMCPLGADEPSAMVRRIVRNGIPLDSLETVPAHCPTCDRETEMILYGNGQAGCAT
                     CGSTGRWVIDHLEPA"
     gene            1006..1488
                     /locus_tag="VFU45_03020"
     CDS             1006..1488
                     /locus_tag="VFU45_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358068.1"
                     /translation="MRFHATALLAMALAACTGRNPRDPAPGDPAILAGSWEIIAHRDS
                     QPDLHVAVTLAPSAPNDPDVPRALKGGTLEGHFQLADRAWLPTPPTDSGVSAFIGSDS
                     SVIVYLRLEGRCSDCGNLGFAGRFAHDAILGHWSQEMTTTSPQGTFAMHRAPADTTHR
                     "
     gene            1485..1970
                     /gene="tsaA"
                     /locus_tag="VFU45_03025"
     CDS             1485..1970
                     /gene="tsaA"
                     /locus_tag="VFU45_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEU5358069.1"
                     /translation="MITFRPIGTIRSPYRDPGQVPRGPGAVHEAAGTIELLPEFAPGL
                     TDIEGFSHLYVVWVFDRSEGYDLLSTPPTDDRPHGVFATRTPRRPNPIALTVVRLLGR
                     DGATLRVAGVDMLDGTPVLDIKPYLSNVPADQLKRGWMAEAEERRAADSAAPDRTGRN
                     R"
     gene            2047..2499
                     /locus_tag="VFU45_03030"
     CDS             2047..2499
                     /locus_tag="VFU45_03030"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEU5358070.1"
                     /translation="MNRAAGLALGWCLLATPLAGQAEGADSGSVMVTITGIRAEQAGT
                     LLVALYDHASAWLILDSARAVRRIPVTTDSAAAVFDPLPADTGYAIAVIHDRNGNGRL
                     DMRWFPFPRPKEGSGVSNDHVRMGKPSYDDARFAVSGAPRPVRIRLRY"
     gene            2521..2961
                     /locus_tag="VFU45_03035"
     CDS             2521..2961
                     /locus_tag="VFU45_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358071.1"
                     /translation="MSMGTSLEAPAPSTSPRQVTLAWLAPIAMLVVAYAATWVTDWMN
                     DFGATRHLVILYPKQVVFAINLLVVGGAALLYARLERLHPRLRSWLRYTALLIPLWVV
                     FLSGARSAGILADWMRILFVAVSGAMVGIMLALWARMGGVGGDK"
     gene            3163..3681
                     /locus_tag="VFU45_03040"
     CDS             3163..3681
                     /locus_tag="VFU45_03040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU5358072.1"
                     /translation="MKEASERRTTPAVQDFYPDDFAHCYGCGRLNASGLHVRSVWEGE
                     ETVARFAPRPEHIAVPGFVYGGLLASLIDCHAMGTASGAALRAEGKAVGEAPSPRYVT
                     ASLKVDYLKPTPHGAELELRARVTERSERKAVVVVSVAAGGVITARGEVVAVRMPETM
                     RQGGDPNPVKER"
     gene            3683..5578
                     /locus_tag="VFU45_03045"
     CDS             3683..5578
                     /locus_tag="VFU45_03045"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5358073.1"
                     /translation="MMRHPLAALVAAALLGGTHGGPATRPAQAPPVAFNDNRIPAGHL
                     RAGVLSVSLVAAPGDWRPFGADKRGLTIPVFGEEGKPLQDPGPLLRVPLGTRVEVRLR
                     NRTATRLVVHGLSARRVAVMDSLVLAPGASGIARFTADAEGTYYYWGATHGEDFEHRE
                     LDDAHLNGALIVDQAGRRPPTADRVFVIERNMADTLPDGGPDFFHDLFTFNGRPWPHT
                     ERLTYNVGDSVHWRIINATNDVHPLHLHGFYFRVNARGDIARDTIYWRAQERYAVTEP
                     LWDGTTMDMAWRPDRPGGWIFHCHLNPHVAQNTGLGPDTEPTPARVHHLLAGYPMPPG
                     ANHAEMAMGGLVLGIRVRPAPGWRPYAGARRTLRLLVQSDSTAADTTRRFGYVLQEGD
                     HPPAPDSVRVPGPAIVLHRSEPTRIWVVNHTPEMTQVHWHGLEIESFYDGVAGLSGMD
                     SAIAPPIMPGDSFQVLVTPPRAGTFIYHTHINDIRQQTHGLYGPLIVLDSGQAWNPDS
                     DRVYTIGNVPGHLAVNGGTTLAPATLKVGVPYRFRFINITITSPGMHLRMMRNGAPAQ
                     WTPLAKDGRALPPWQRTPVDARQPINIGETYDFGVQSADTGTMSLEVWRADGTILLTR
                     QLLRFER"
     gene            5837..6265
                     /locus_tag="VFU45_03050"
     CDS             5837..6265
                     /locus_tag="VFU45_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358074.1"
                     /translation="MTAFLSTLRLRRRRLAGLLLLLFLPSCNSWQVAQPTPAAYLQNE
                     RPASARVTRTDGTRLVVRTPSLAVDTLQATSDDGKATAVAVPLDKVARLEVRKFSAGR
                     TLLLVGGIIAAVSTIAAASLDYGFGGGFNLGSPTRPPAAP"
     gene            complement(6262..>6813)
                     /locus_tag="VFU45_03055"
     CDS             complement(6262..>6813)
                     /locus_tag="VFU45_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358075.1"
                     /translation="PWAPAGAYTVQLTVNGKRYTQPLRLYLDPRVRTPAPALAELAAL
                     THEMYLGARAAHQDAVRAHALAAALTTADGEGVAGLRAALDSIAPAPRPEGRRGFRGR
                     RNGAKAAPTLDAVSGEMLGAAMAMQEADVAPTAENVAACARARADAAEVLRKWNALVD
                     GALPRLNARRKAAGLPAVTLPRD"
BASE COUNT          980 a         2597 c         2276 g          960 t
ORIGIN      
        1 tggcgcaagg tcgacctcac ccgcgacgcc gccggcgccc gggtccggct caacgaccgc
       61 gacatgcacg tcgacctcgg ggggatcgcc aagggctacg gcatcgaccg cgccgtggcg
      121 gccatccggc ggctcggcat cgcccacgcc atcgtgaccg tgggcggcga cctctacgcg
      181 ctcgggccct cgccgagcgg tgaaccgtgg gaggtcggga tccgcgaccc gcaccacctc
      241 gaccagctcg ccgggcgcct cgaggtatcc gaccgcgccg tcaccacctc gggcgactac
      301 gagcgcttct tcgagtatca cggggtgcgc taccaccatc tcatcgatcc ggtcaccgcg
      361 gcgccccgcc gcaccggcgt gcacagcgtg acggtcctcg gcgccgactg cttcaatgcc
      421 gacgccgcgt ccaccgccgc cttcggcctc gaccgcgaca ccgcgcgccg gatggtccgc
      481 cgcgtgctga gcgacgccga ggtcattccc ctgacgtgac gtggagggca gcatgaaaag
      541 ccaccagctg tactgcgggg cctgcgaccg gccggtccgg gtgctcatca ccgagcaggc
      601 cctgggcgag acgcaggccc ccgtccgcga cgccgagttc atctgcctcg agatcggcac
      661 cacctgcacc ggccggatgt gcccgctggg cgcggacgag ccgagcgcga tggtgcggcg
      721 catcgtccgg aacgggatcc cgctcgactc gctcgagacc gtccccgcgc actgccccac
      781 ctgcgaccgc gagactgaga tgatcctcta cggcaacgga caggcgggat gcgccacctg
      841 cggcagcacc gggcgctggg tcatcgacca tctcgagccg gcgtgaggtc ggccggccgc
      901 acccctttcc gggttgcgct cccgtccggc acggtggaca ttcttgcgct cccgacccgg
      961 ccgtcgacgc cggccaactc ccggcccaga ccgatgagag gcacgatgcg attccacgcg
     1021 accgcgctcc tggcgatggc cctcgccgcc tgcaccgggc gcaacccccg cgacccggcc
     1081 cccggcgatc ccgcgatcct ggccggctcc tgggagatca tcgcccatcg cgacagccag
     1141 cccgacctgc acgtggcggt gacgctcgca ccctcggcgc cgaacgaccc cgacgtcccc
     1201 cgggcgctca agggcgggac gctcgaaggc cacttccagc ttgccgaccg cgcctggctc
     1261 ccgaccccac cgaccgattc cggggtctcg gccttcatcg gctccgacag ctcggtcatc
     1321 gtctacctcc ggctcgaggg gcgctgcagc gactgcggca acctcggctt cgccggccgg
     1381 ttcgcgcacg acgccatcct ggggcactgg agccaggaga tgaccaccac gtcgccccag
     1441 ggcacgttcg cgatgcaccg cgccccggct gataccaccc atcgatgatc acgttccgtc
     1501 cgatcggcac catccggagc ccctaccgcg acccgggtca ggtcccacgc ggcccgggcg
     1561 cggtacacga ggccgcggga acgatcgagc tcctgcccga attcgcgccg ggcctgacgg
     1621 acatcgaggg gttctcccac ctctatgtcg tctgggtctt cgatcggtcc gaggggtacg
     1681 acctgctctc gacgccaccc accgatgacc gcccccacgg cgtctttgcc acccggacgc
     1741 cgcggcgccc gaaccccatc gccctcaccg tggtgcggct cctcggccgg gatggtgcga
     1801 cgctccgcgt ggccggcgtc gacatgctcg atggcacgcc ggtgctcgac atcaagccgt
     1861 acctgagcaa cgtccccgcg gaccagctca agcgcgggtg gatggcggag gccgaggagc
     1921 gccgtgccgc agactccgcc gcccccgacc gcacgggccg caatcgatga cgttggacgc
     1981 caccggcact tgccgctctg cgagcgaggc gcgccggctc tatcccgact acaccgaccc
     2041 gacgccgtga accgcgcggc cgggctcgcg ctcgggtggt gcctccttgc gaccccgctc
     2101 gccggccagg cggagggggc cgattccggc tcggtgatgg tcaccatcac cggcatccgc
     2161 gccgagcagg cggggacgct cctggtggcg ctttacgacc acgcctcggc gtggctcatc
     2221 ctcgactccg cgcgcgccgt ccggcggatc ccggtcacca ccgactcggc cgccgcggtc
     2281 ttcgacccat tgccggccga cacgggatat gccatcgccg tcatccacga ccggaatggc
     2341 aacggcaggc tcgacatgcg gtggttcccc ttccccaggc cgaaggaagg gtccggcgtt
     2401 tccaacgacc acgtgcggat gggaaagccg agctacgacg atgcccgctt cgccgtgagc
     2461 ggtgcccccc ggccggtccg gatccgcctc cgttactagc gcccgcggga cggggtatca
     2521 ttgagcatgg gaacctccct cgaagcaccc gcgccctcga cgtccccccg ccaggtgacg
     2581 ctggcgtggc tcgccccgat cgcgatgctc gtggtggcgt acgccgccac ctgggtcacc
     2641 gactggatga acgacttcgg cgccacccgg cacctcgtca tcctgtatcc caagcaggtc
     2701 gtcttcgcca tcaacctcct cgtcgtcggc ggggcggcgc tcctctacgc ccggctcgaa
     2761 cgcctgcatc cgcggctgcg gagctggctc cgctacacgg cgctgctcat tccgctctgg
     2821 gtcgtgttcc tctccggcgc ccgctcggcc gggatcctgg ccgactggat gcggatcctg
     2881 ttcgtggcgg tgagcggggc gatggtcggg atcatgctgg cgctgtgggc gcggatgggg
     2941 ggcgtggggg gtgataagtg aagagtgaaa agggaaaagt gaaaagggag aaggagaagg
     3001 aggagggaga aggagaaggg tgaaggaaaa gtgagaatga aggcgtgaga ggtgaggggt
     3061 acgaggcgag aaggacgtga ggcgcccccc ggcgctggcg ccgatgtccg ggacgcgtag
     3121 cttccagcag gcagtgcatc ctcccgcgag atgaccacac cgatgaagga agcttccgaa
     3181 cgccgcacca cgccggcggt ccaggacttc taccccgacg actttgcgca ctgctacggg
     3241 tgcggccggc tcaatgcgtc cggcctgcac gtgcgctcgg tctgggaggg cgaggagacg
     3301 gtggcccgct tcgcgccccg gcccgagcat atcgcggtcc cgggcttcgt gtacggcggc
     3361 ctcctcgctt cgctcatcga ttgccacgcg atgggcaccg cctccggggc cgcgctccgc
     3421 gccgaaggga aggccgtggg cgaggcgccc tcgccccggt acgtgactgc ctccctcaag
     3481 gtggactacc tcaagccgac gccgcacggc gccgagctcg agctccgcgc ccgcgtcacc
     3541 gagcggagcg agcggaaggc ggtcgtcgtg gtgtccgtgg cggccggcgg cgtgatcacg
     3601 gcccgcggcg aggtggtggc ggtgcggatg cccgagacga tgcgacaggg cggcgacccg
     3661 aacccggtga aggagcgtta gcatgatgcg acatccgctc gcggccctcg tggccgccgc
     3721 actgctcggc ggcacccacg gcggcccggc cacccgcccc gcccaggcgc cgccggtcgc
     3781 gttcaacgac aatcgcatcc ccgccgggca cctccgcgcc ggagtgctct cggtgtcgct
     3841 ggtggccgcc ccgggcgatt ggcgcccctt cggcgccgac aagcgcggcc tcaccatccc
     3901 cgtcttcggc gaggagggca agccgctcca ggaccccggc cctctcctcc gcgtgccgct
     3961 cggcacccgc gtcgaggtcc ggctccggaa ccggaccgcc acccggctcg tggtccacgg
     4021 gctctcggcc cggcgggtcg ccgtgatgga ctcgctggtg ctggcacctg gcgccagcgg
     4081 catcgcgcgc ttcaccgccg acgcggaggg gacctactac tactggggcg ccacccacgg
     4141 ggaggatttt gagcaccggg agctcgacga cgcgcacctc aacggcgcgc tgatcgtcga
     4201 ccaggcaggg cggcgcccgc cgaccgcgga ccgcgtgttc gtcatcgagc ggaacatggc
     4261 cgacaccctg ccggacggcg gccccgactt cttccacgac ctcttcacct tcaatggacg
     4321 cccgtggccg cacaccgagc gactcaccta caacgtgggc gattcggtgc attggcggat
     4381 catcaacgcc accaacgacg tccacccgct ccatctccac ggattctact tccgggtcaa
     4441 cgcacgcggc gacatcgctc gtgacaccat ctattggcgg gcgcaggagc ggtacgccgt
     4501 caccgagccg ctctgggacg gcaccacgat ggacatggcg tggcgacccg accgccccgg
     4561 cggctggata ttccactgcc acctcaatcc tcacgtcgcg cagaacacgg ggctcgggcc
     4621 tgacaccgaa ccgacgccgg cccgggtcca tcatctgctg gccgggtacc cgatgccgcc
     4681 gggcgctaat cacgccgaga tggccatggg cgggctggtc ctcgggatcc gcgtgcgccc
     4741 ggcgccgggt tggcgtccgt atgcaggcgc ccggcggacg ctccggctcc tggtccagtc
     4801 cgattccacg gcggccgaca ccacgcgccg cttcgggtac gtcctgcagg aaggcgatca
     4861 cccgcccgcc cccgactccg tgcgcgtccc cgggcctgcg atcgtgctcc accgcagtga
     4921 accgacgagg atctgggtcg tgaatcacac gccggagatg acgcaggtcc actggcacgg
     4981 gctcgagatc gagagtttct acgacggcgt ggcgggactg agcgggatgg attcggcgat
     5041 cgccccgccg atcatgccgg gcgattcgtt ccaggtgctg gtgaccccgc cgcgggcggg
     5101 gacgttcatc taccacaccc acatcaacga catccgccag cagacgcacg ggctctacgg
     5161 gccgctgatc gtgctggact cgggccaggc ctggaacccc gactcggacc gggtgtacac
     5221 catcggcaac gtgccgggcc acctggccgt gaacggcggc accacgctcg ccccggcgac
     5281 gctcaaggtc ggcgtgccgt accggttccg gttcatcaac atcaccatca cgtcgccggg
     5341 gatgcacctg cggatgatgc ggaacggcgc gcccgcccag tggacgccgc tcgcgaagga
     5401 cggccgggcg ctcccgccct ggcagcgcac gccggtcgac gcccgccagc ccatcaacat
     5461 cggcgagacg tacgacttcg gcgtccagtc cgccgacacc ggcaccatgt cactggaagt
     5521 ctggcgtgcc gacggcacga tcctgctgac ccgtcagctg ctccggttcg agcgatgact
     5581 gcgatgaggc cgcgcgggag cagagcctcg cgcggcgcgc ggcgaggtgc tggcggttcg
     5641 aatgccggac tcgatgggcc gggaggggtg atcgcccctc cggcaccgtc accgccgcgg
     5701 cgccgccaca cctcccgcgc cgcgggtcag ccgaccttcc tcgcaggttc acatcttctt
     5761 ctgttcgaca cgtcgatctt gcgatcctct cgaaaccacc ggccccgact cgttcggtgc
     5821 cgggacgggg gaaccgatga cggcgttcct ttccacactc cgactccgca gacggcggct
     5881 ggccggcctg ctgcttctgc ttttcctccc gtcctgcaac agttggcagg tggcccaacc
     5941 gacacccgca gcctacctcc agaacgaacg cccggcgtca gcgcgcgtca cccggaccga
     6001 tgggacgcgg ttggtcgtcc gaacgccgag cctcgcagtc gacacgttgc aggcgacctc
     6061 ggacgatgga aaggcgacgg ccgtcgcggt gccgctcgac aaggtggcgc gccttgaggt
     6121 gcgcaagttc tccgccggac ggacgctcct gctcgtcggc gggatcatcg cggccgtgtc
     6181 aacgatcgcc gcagcgagcc ttgactatgg ctttggcggc ggcttcaacc tcggctcccc
     6241 aaccagaccg ccggctgcgc cctagtccct cggcagcgtc accgccggca gccccgccgc
     6301 cttccggcgg gcgttgagcc gcggcagcgc cccgtccacc agcgcattcc acttccgcag
     6361 cacctccgcc gcatcggccc gggcgcgcgc gcacgccgcc acgttctccg ccgtcggcgc
     6421 cacgtccgcc tcctgcatcg ccatcgcggc acccagcatc tcgccgctca ccgcgtcgag
     6481 cgtgggcgcg gccttggccc cgttccggcg gccgcggaac ccgcgccgcc cctcgggcct
     6541 gggcgccggc gcgatcgaat cgagcgccgc ccgcaagccc gccaccccct cgccgtccgc
     6601 cgtggtgagc gcagccgcca gcgcatgggc ccgcacggcg tcctggtgcg ccgccctggc
     6661 gcccaggtac atctcgtggg tgagcgccgc gagctccgcc agcgccggcg ccggcgtcct
     6721 gacccgcggg tcgaggtaga gccggagcgg ctgggtgtac cgcttgccat tcaccgtgag
     6781 ctggaccgtg tacgcgccgg cgggcgccca cgg
//
LOCUS       DASUAP010000128         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_276072, whole genome shotgun sequence.
ACCESSION   DASUAP010000128 DASUAP010000000
VERSION     DASUAP010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276072"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..234
                     /locus_tag="VFU45_03060"
     tRNA            140..234
                     /locus_tag="VFU45_03060"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:174..176,aa:Sec,seq:tca)
     gene            332..1228
                     /locus_tag="VFU45_03065"
     CDS             332..1228
                     /locus_tag="VFU45_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU5358076.1"
                     /translation="MPTALLYNPAAGRGLAPKLRDRALRAAGAHWPRIELLETREPGD
                     GVRLAREAATAGIDLLLVLGGDGTVHEAANGVRAAGAAAPRIGVLPCGTGNDFAKLVG
                     TARCRPEEAVRRLAAGAPATFDTGEAWGERFVNSAGVGLDAEVAHELRTVKRLRGTAA
                     YAVALLRTLRSFRALELEVSVAGERWTGRWTAVVLGNGQVEGGSFRLTPDARPDDGQL
                     DLCAVSALGTARLLTLIPTLFWGGHGRFREVRLTRVTGVVIRSPGAPLRLHLDGEIRA
                     PGPREVEFRVVPASLPVLIGRR"
     gene            1351..2388
                     /locus_tag="VFU45_03070"
     CDS             1351..2388
                     /locus_tag="VFU45_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEU5358077.1"
                     /translation="MPSRFFASDNAAPAHPEVLAALARANEGHAVAYGADPWTARATE
                     LILAELGGEAQVFFVFAGTAANVLGLTALTRRYGAVICSADAHIEQDETGAPERHLGV
                     KLIDLPTPDGKLRLEQLPPLLAGRGVEHHVQPDVLALTQSTERGTVYRIEELRALCDW
                     AHRDGLRVHVDGARLANAAVTLGVGLGAISREVGVDALSLGGTKNGLMGAEAVVFFDP
                     AVAEGFRYLRKQGMQLASKHRFLAAQFEALFTGGLWRRSAEHANAMAQRLAAAVREVK
                     GVSVTQPVEANGVFARLPDRVIVPLRDAFPFYVWRQGSDGTSEVRWMCSWDTTEADVD
                     AFVGRLRELSR"
     gene            2385..2933
                     /locus_tag="VFU45_03075"
     CDS             2385..2933
                     /locus_tag="VFU45_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358078.1"
                     /translation="MRIITPGPRRVALLVCGLVLARPLMAQQPDTGAVHPPAADSLRR
                     AAPDSIVPTRPQAPDSTLLKPPITPMGAFFRSLAVPGWGQATLGRKLTGGIMVAWEGV
                     TLGMTVKTSHELAYYENIHSPNIPAKEQERQDWLVLLVFNHLFSGLEAYVSAHLWDFP
                     PDLHLQALPHGVGGSISLPPVR"
     gene            2933..3733
                     /gene="murI"
                     /locus_tag="VFU45_03080"
     CDS             2933..3733
                     /gene="murI"
                     /locus_tag="VFU45_03080"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU5358079.1"
                     /translation="MHSGPIGIFDSGIGGLTVTRAIYEALPHESTVYFGDTARVPYGP
                     KSPETVRRYSLEILHWLLEQGVKAVVVACNTSTAHALDALRAAAPVPVIGVIEPGAAA
                     AVRAARRGPIGVIGTAGTVASGAYARAIQARRPGTDVRQVACPLFVPLVEEGWFDHPA
                     TELVAREYLAPLVAAQVDALVLGCTHYPLLKPLLGRVMGPSVALIDSADETALETRRV
                     LEEADLLAPAGHRTTHRFAVSDDEARFLQVGARFIGERLGGADVVRLG"
BASE COUNT          525 a         1407 c         1370 g          605 t
ORIGIN      
        1 ggactgcccc ggggtgcccg cgtcgagatc gaagtggtcg cagcgaagtg aacggtgaac
       61 ggtcaacggt gaacgggggt gctcctgagt caggccgcat cccgttgacc gttgaccgtt
      121 gaccgttcac cgcaatgaag gggacgcatc gggtctggtg acccggccgg tcttcaaaac
      181 cgcgacggag gcgcctcagg cggctccggt gggttcgatt cccacacgtt cccgtgccgg
      241 tgagtaagcg gtgaacggtc aacggtgaac ggggctgctc cgatgtggtc cgttcccgtt
      301 gaccgttgac cgtttaccgt tcacccctca aatgcccacc gccctccttt ataaccccgc
      361 cgccgggcgg ggactcgccc cgaagctccg cgaccgcgcg ctccgcgccg ccggggcgca
      421 ctggccccgc atcgagctcc tggagacccg ggagcccggc gacggcgtgc gcctggcgcg
      481 cgaggcggcc accgccggca tcgacctcct cctggtcctc ggtggcgacg gcaccgtcca
      541 tgaggcggcg aacggcgtcc gggccgctgg ggcggcggcg ccccggatcg gtgtcctccc
      601 ctgcggcaca gggaacgact tcgccaagct cgtcggcacc gcgcgctgcc ggcccgagga
      661 ggcggtccgg cggctcgcag caggcgcccc cgccacgttc gacaccggcg aggcgtgggg
      721 agaacgcttc gtcaacagcg cgggggtggg gctcgacgcc gaggtggccc acgagctccg
      781 caccgtcaag cggctgcggg ggacggccgc ctacgccgtc gcgctgctgc gtacgctgcg
      841 ctccttccgc gcgctcgaac tcgaggtgtc ggtcgccggt gagcgctgga cggggcggtg
      901 gaccgccgtc gtgctcggga atggccaggt cgagggcggc tcgttccgcc tcacccccga
      961 tgcccgtccc gacgacggtc agctcgacct gtgcgccgtg tcggcacttg gcacggcgcg
     1021 cctccttacc ctgattccga ccctcttctg gggtgggcac ggccgcttcc gcgaggtgcg
     1081 cctgacccgc gtcaccggcg tggtcatccg ctcgcccggt gcgccgctcc gcctccacct
     1141 cgacggcgag atccgcgccc ccggcccgcg tgaggtcgag ttccgggtcg tccccgcgag
     1201 cctgccggtg ctgatcggcc gccgctgaag agtggagagt gaaaagtgaa cagtgagaag
     1261 tgaagaggac cgccccgttc gcgtgatagt gcttcttgac tcgttactgc actcttcact
     1321 cttcactctt cactctttac tcttccaccc atgccgtccc gcttctttgc cagcgacaac
     1381 gccgcgcccg cgcatcccga ggtgctcgcc gcgctggcgc gcgccaatga gggacacgcc
     1441 gtggcctacg gcgccgatcc ctggaccgcg cgcgcgacgg agctgatcct ggccgagctc
     1501 ggcggcgaag cgcaggtctt cttcgtcttc gcgggcaccg cggccaacgt cctcggcctc
     1561 accgccctca cccggcgcta cggcgccgtc atctgctccg ccgacgcgca catcgagcag
     1621 gatgagaccg gcgcccccga acggcacctc ggcgtcaagc tgatcgacct tccgaccccc
     1681 gacgggaagc tccggctcga gcagctcccg ccgctcctgg cggggcgggg ggtggagcac
     1741 cacgtgcagc cggacgtgct ggcgctcact cagtccaccg aacgcggcac ggtgtaccgg
     1801 atcgaggagc tgcgggcgct ctgcgattgg gcgcaccggg acgggttgcg ggtccacgtc
     1861 gatggcgccc ggcttgccaa cgcggcggtg acgctcggcg tggggctcgg ggccatcagc
     1921 cgtgaggtcg gcgtcgacgc cctgtcgctg ggggggacca agaacggcct gatgggcgcc
     1981 gaggccgtcg tcttcttcga tccggcagtg gcggaggggt tccggtacct gcggaagcag
     2041 gggatgcagc tcgcgtcgaa gcaccgcttc ctcgcggcgc agttcgaggc gctcttcacc
     2101 ggtggactct ggcgccggag cgccgagcac gcgaatgcga tggcgcagcg gctcgccgcc
     2161 gcggtccggg aagtgaaggg cgtctccgtc acccagccgg tcgaggccaa cggcgtcttt
     2221 gcccggctgc ccgatcgggt gatcgtgccg ctccgcgacg cgttcccgtt ctacgtctgg
     2281 cgccagggga gcgacgggac gagcgaagtg cgctggatgt gctcgtggga caccaccgag
     2341 gcggacgtgg acgcgttcgt gggccggctc cgggagcttt cccggtgagg atcatcaccc
     2401 ccggtccccg ccgggtggcg ctcctggtgt gcggtctcgt gctggcgcgc ccgctcatgg
     2461 cgcagcagcc cgacaccggc gccgtgcatc cgccggcggc cgattcgctg cggcgcgccg
     2521 cacccgactc gatcgtgccg accaggcccc aggcgccgga ctcgacgctc ctgaagccgc
     2581 cgatcacgcc gatgggggcc ttcttccggt cgctcgcggt gccgggatgg ggtcaggcca
     2641 ccctggggcg caagctcacc ggcgggatca tggtcgcgtg ggaaggggtc acgctgggaa
     2701 tgacggtcaa gaccagccac gagctggcgt attacgagaa catccattcg ccgaacatcc
     2761 cggccaagga gcaggagcgg caggattggc tggtgctcct ggtcttcaac cacctcttct
     2821 ccggactcga ggcgtacgtg tcggcccacc tctgggactt cccgcccgac ctccacctcc
     2881 aggcgctgcc ccacggcgtg ggcggctcca tctccctgcc gccggtgcgc tgatgcactc
     2941 cggccccatc ggcatctttg attccggcat cggcgggctc accgtgaccc gcgcgatcta
     3001 cgaggcgctg ccgcacgagt ccacggtcta tttcggtgac accgcgcgcg tgccctacgg
     3061 ccccaagtcg cccgagaccg tgcgacggta ctcgctcgag atcctccact ggctccttga
     3121 gcagggcgtc aaggcggtcg tcgtcgcctg caacacgagc acggcgcacg cgctcgatgc
     3181 cctgcgcgcc gcggcgccgg tcccggtcat cggcgtcatc gaacccggcg cggccgcagc
     3241 ggtgcgcgcc gcccgccgcg ggcccatcgg cgtcatcggc accgcgggca ccgtggcgag
     3301 cggcgcctac gcccgcgcca tccaggcccg ccgtcccggc accgacgtcc ggcaggtggc
     3361 gtgtcccctc ttcgtgccgc tggtggagga agggtggttc gaccacccgg ccaccgagct
     3421 ggtggcgcgc gaatacctgg cgccgctcgt cgccgcccag gtcgacgcgc tggtgctcgg
     3481 ctgcacccac tacccgctcc tcaagccgct gctcgggcgg gtgatggggc cgagcgtggc
     3541 cctgatcgac agcgccgacg agacggcgct ggagacgcgg cgggtgctgg aggaggcgga
     3601 cctgctggca ccggccggcc accgcaccac gcatcgcttt gccgtgagcg acgacgaggc
     3661 gcggttcctg caggtcgggg cccgcttcat cggggagcgg ctcggcgggg cggatgtggt
     3721 gcggctggga taggggcgcc tccggaaacg gtcaagcgca ccggtcacta aggcgtcgca
     3781 aagatcgacc actagggttt tccctagtgg tctttcaatt gttccattct catattgcag
     3841 cttcccctga ccgagcagag cctgtcgatg aagcgcgaac cgatgtggat cgccgccaag
     3901 aaggcga
//
LOCUS       DASUAP010000129         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_453698, whole genome shotgun sequence.
ACCESSION   DASUAP010000129 DASUAP010000000
VERSION     DASUAP010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453698"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1944
                     /locus_tag="VFU45_03085"
     CDS             <1..1944
                     /locus_tag="VFU45_03085"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU5358080.1"
                     /translation="GDLDPATRAMLDHYVAGINAWISRARMLPPEFVLLRTKPAPWTL
                     DDIAAVAIYQTYFASALMDRDRDYRDLLMRLGPVGAKLVGAYDAWSPATVPGRDLALG
                     ADRYGMALASNSWVIAPSRSASGAAIHESDPHLQVDQAPALWYIAGLRTKDGLDVVGV
                     TAPGLPFVAMGHNAAIAWAFTVAPVDLADEYREHFAPDGPLRVRTPDGWAPVQVIPES
                     IAVKGEARPRILQVLETPRGVVTARQGDSGVSLHWAGFDFSAATLLAHGDSLMRATDF
                     GQFRRAVTGFAALSVNWTYSDVHGHIGYQLGTPIPIRDTANTFLLRDAADHGAGWRGY
                     RTLGDTPFAYDPPAGWLATCNNQPVPANWPYPLPGFYDDYRIVRAASLLSAQQRWTPE
                     DVRQMQGDIMSTGALRFRALLADGADQAGRHDLAARIRAWSGVATADDTLSALYAYWR
                     RELTRALFEDELGTRWPEGDPLVDGVVTGNVVSVIDDHRTPQVETAADISARALSVAL
                     AQVRGRRLGQVQTLTIRHPLAVSAVLDRWLHLTRGPYAMGGDNTTLDASYRAYDPATG
                     TFRVIVGPSMRFVLDWARPDAFSMTIPLGESGNPLSPHFDDFFGIHRDDGRWTVPFSR
                     DSVLSRAASTLRLEPAADPKIHR"
BASE COUNT          320 a          801 c          705 g          342 t
ORIGIN      
        1 ggagacctcg accccgccac ccgcgcgatg ctggaccact acgtcgccgg catcaacgcg
       61 tggatctccc gtgcccggat gcttccgccg gagttcgtac tcctccggac gaagccggcg
      121 ccgtggacgc tcgacgacat cgccgccgtc gcgatctacc agacctattt cgcgagcgcg
      181 ctgatggacc gcgaccgcga ctatcgggac ctcctgatgc gcctcggccc cgtgggcgcc
      241 aagctcgtcg gcgcatacga tgcgtggtcg cccgccacgg tgcccggccg cgacctggcc
      301 ctcggcgctg accgctacgg gatggcgctc gcctccaatt cgtgggtcat cgcgccgtcg
      361 cgctcggcga gcggtgccgc gatccacgaa tccgatccgc acctgcaggt cgaccaggcc
      421 ccggcactct ggtacatcgc ggggctccgc acgaaggacg gcctcgatgt ggtcggcgtg
      481 accgcgccgg ggctcccgtt cgtggcgatg ggccacaacg cggcgatcgc ctgggcattc
      541 accgtggcac cggtcgacct ggcggacgag taccgggaac acttcgcgcc ggacggcccg
      601 ctccgcgtcc gcacgccgga cggctgggct cccgtgcagg tcatccctga gtcgatcgcc
      661 gtcaagggcg aggccaggcc ccgcatactc caagtgctgg agacaccgcg cggcgtggtg
      721 accgcgcgcc agggcgattc gggggtgtcg ctccactggg cggggttcga tttttcggcg
      781 gccacactcc ttgcccacgg cgattccctg atgcgcgcca cggacttcgg ccagttccgc
      841 cgggccgtga ccggcttcgc ggcgttgagc gtcaactgga cctacagcga cgtccacggg
      901 cacatcggct accagctcgg cacgccgatc ccgatccgcg acaccgcgaa caccttcctc
      961 ctccgcgacg ccgccgacca cggggccggc tggcgcggct accgcacgct cggcgacacg
     1021 ccctttgcct acgatccgcc ggcgggatgg cttgccacct gcaacaacca gccggtgccc
     1081 gccaactggc cctacccgct gccgggattc tatgacgact accgcatcgt gcgcgcggcg
     1141 tcgctcctct cggcacagca gcgctggacc ccggaggacg tgcggcagat gcagggggat
     1201 atcatgtcga ccggggcgct gcgcttccgg gcgctcctgg ccgacggcgc ggatcaggcc
     1261 ggccggcacg acctcgcagc gcggatccgg gcgtggagcg gcgtggccac cgcggacgac
     1321 acactttccg cgctgtacgc ttactggcgg cgggagctga cccgtgcgct cttcgaggac
     1381 gagctcggca cgcgctggcc ggaaggggat ccgctggtgg acggtgtcgt caccggcaac
     1441 gtcgtcagcg tcatcgacga ccaccgcacg ccgcaggtgg agaccgcggc cgacatctcg
     1501 gcgcgggcgc tctccgtcgc actcgcccag gtgcgggggc gtcgactcgg ccaagtgcag
     1561 accctcacga tccggcatcc gctggccgta tcagctgtcc tggaccgctg gctgcacctg
     1621 acccgaggcc cgtacgcgat gggcggggac aacaccacgc tcgacgcgag ctaccgtgcg
     1681 tacgacccgg ccaccggcac gttccgggtc atcgtcgggc cgagcatgcg cttcgtgctc
     1741 gactgggccc gtcccgacgc cttctcgatg accatcccgc tcggcgaatc ggggaatccg
     1801 ctgagtccac acttcgacga cttcttcggc atccatcgcg acgacggacg gtggactgtt
     1861 ccgttcagcc gggactcggt cctttcccga gcggccagta cgcttcgact cgagccggcg
     1921 gcggatccca agatccacag atgacgcaga ttacgcagat gctcgcgtgg cgatcttcgt
     1981 ccgttgcatt ctggagggcg caataacgaa gccgatcgtg atgtcatcat ctgcgacatc
     2041 tgcagcacct gtggatataa cctgcgtcat ccgcggacct gcgctttcct gctcctctcc
     2101 gcccaagaac gatgcctttc cggcgaccgg acctcgcaag cttcgggggc catgtccacc
     2161 gacgccca
//
LOCUS       DASUAP010000130         4787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_289282, whole genome shotgun sequence.
ACCESSION   DASUAP010000130 DASUAP010000000
VERSION     DASUAP010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289282"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2254)
                     /locus_tag="VFU45_03090"
     CDS             complement(<1..2254)
                     /locus_tag="VFU45_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358081.1"
                     /translation="MTTLVALSAARMGAQAPAALPTPTPIHWRTGPLDFTGSARAGTY
                     VSATGHRAIVMGDELGHLEAWAWPLKLLHGFQLRFQTPFYAEPIAASELARRVDITPA
                     GFTITYTHPAFTVRERVFVAEDDPAAVIVLEVDAVRPIEILAEFQSDLQLAWPGAVGG
                     QYVYWDDAERAFVLAESRRQYSAMVGSPYTTSATNQPAHNVPEAPNQLRIAVGDQGAV
                     PMPRPGEPEGRLVHVHASGIPIVIVGGAMPRDSVRALYDRVLASVPALYDARVAHAAA
                     VTDSAVSLRSGDADFDRAIAWAALDLDAAMVCNPDLGCGLVAGYGPSGARGARPGFGW
                     FFGGDASINSLATTAAGEWDLTRRGLEFFAKYQRADGKIAHEISQAAGRIDWFKSYPY
                     PFYHADTTPYWILAWGEYWLTTGDTASLRRNWAAIRKAYEWSRSTIDPATGLMLNSKG
                     GLGAVEVGDLGLGVQSDIYLSGVWVRALDRMARMADAMGEKRLPAEARALGERALTSL
                     RERFWLPAVGRHAFALLDSGQTRPELTVWPSIPLALGLFADDHAFAEGAAQARATLVT
                     DWGARSLASESHLFDPMHYNNGTVWPFVTGWSALGQFAARDPYAGWTELQSIANAGRT
                     MALGHQPEVWSGTTFEPLETAVPQQFFGTSFLLTGLVRGILGWAPDVPAGRIVFAPQI
                     PWVGRDVQVDRLRAGPGAWDVRLRREAGALDVSMHRRGGGPGQVRFSPVLPAGADSVR
                     VMEDGRALDCA"
     gene            2533..2856
                     /locus_tag="VFU45_03095"
     CDS             2533..2856
                     /locus_tag="VFU45_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358082.1"
                     /translation="MAELSDMEMGWSGAGWMDPAPMPSTPEPAPMAPMTPAAPAPAAP
                     RSKPAAKKPARKKAAKKKPAKKKAAKKPRKKVARRKPARKAARKRPAKRKAATRAKKK
                     GKKRR"
     gene            2950..4299
                     /locus_tag="VFU45_03100"
     CDS             2950..4299
                     /locus_tag="VFU45_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5358083.1"
                     /translation="MRKASLFVLSLALAGCGKPVKDRIALVGVTVIDGTDAPARVDQV
                     MIIRGSRIDTIAPAAGFAIPKTATVIDLKGKWVIPGLIDADGDVDRWTVKRYLAFGVT
                     AVRDVGNDQDSILALAEGAALHSLVSPRLYVTGAALDGGTPFDPSSAALTGESDARRA
                     VDDRVQAGVSFVSTGPGFTPVLLRAVVDEATNLNTSVTARLGLTDARTAGKMGVKSLL
                     ELSGVPEAVVNPEPFYAAYRKAPWNGWTTTERAWAGLDSAPLGAVATDLATAGVTLVP
                     ALVLHDTWSRLDDSAVTASPDLAYVPATARSDWDIPKFEAQAGWNASTYPAFRAGRSK
                     QDLFVREFKLAGGRIAAGTGAARLMLVPGASLHRELELLVNAGLPTDDAIRSATSVAA
                     ALIGADSLGTLEPGKVADLVVLNADPLADIRNTRSIVKVVLGGLVLDADSLARRIRQ"
     gene            4296..4646
                     /locus_tag="VFU45_03105"
     CDS             4296..4646
                     /locus_tag="VFU45_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358084.1"
                     /translation="MTLTRLVLAWGVGAVWYALVDAGLGRYWPAAGVTFRPKVILAET
                     LLLTMFAALWFGSLGHGGWWLLFLVLGALMEGPVRARHRIGAAPGGGRPWLDAAIGIC
                     RVVIAGGLLSWRLS"
BASE COUNT          735 a         1613 c         1812 g          627 t
ORIGIN      
        1 tggcgcagtc gagcgcgcgg ccgtcctcca tcacccgcac gctgtcagca ccggccggga
       61 gcaccggcga gaaccgcacc tggcccggcc cgcccccgcg gcgatgcatc gacacatcga
      121 gcgcgccggc ctcgcgccgg aggcggacat cccatgctcc ggggccagcg cggagccggt
      181 cgacctgcac gtcgcggccg acccagggga tctgcggcgc gaacacgatg cgccccgcgg
      241 ggacatccgg cgcccagccg aggatgccgc gcaccaggcc ggtgaggagg aatgaagtgc
      301 cgaagaactg ctgcggcacc gccgtctcca gcggttcgaa ggtggtgcct gaccacacct
      361 ccggctggtg gccgagggcc atcgtgcgtc ccgcattggc gatcgactgc agctccgtcc
      421 acccggcgta gggatcgcgc gcggcgaact gtcccagcgc cgaccagccg gtcacgaagg
      481 gccacacggt gccgttgttg taatgcatcg gatcgaagag atgactctca gaggcgaggg
      541 aacgcgcgcc ccagtcggtc acgagcgtcg cgcgggcctg ggcggcacct tcggcgaagg
      601 cgtggtcatc ggcgaagagc ccgagcgcaa gcgggatgct gggccagacg gtgagctccg
      661 gacgggtctg cccgctgtcg aggagggcaa aggcgtggcg cccgacggcg gggagccaga
      721 agcgctcgcg gagcgacgtg agggcgcgct cgcccagggc gcgggcctcg gcggggaggc
      781 gcttctcgcc catcgcgtcg gccatccgcg ccatccggtc gagggcgcgg acccagacgc
      841 cgctcaggta gatgtcagac tggacgccga gcccgaggtc gccgacctcg acggcgccca
      901 ggccgccctt ggagttgagc atcaggccgg tcgcgggatc gatggtgctg cgcgaccact
      961 cgtacgcctt gcggatggcg gcccagttcc ggcggaggga cgccgtgtcg ccggtggtga
     1021 gccagtactc cccccaggcg aggatccagt agggcgtggt gtcggcgtgg tagaacgggt
     1081 aggggtacga cttgaaccag tcgatacgcc cggcagcctg cgagatctca tgggcgatct
     1141 tgccgtcggc gcgctggtat ttggcaaaga actcgagccc gcggcgggtg aggtcccatt
     1201 cccccgcggc ggtggtggca agggagttga tcgaggcgtc gccgccgaag aaccagccga
     1261 agccggggcg ggccccacgg gcgccgctcg gcccatatcc ggcgacgagc ccgcagccga
     1321 ggtcgggatt gcacaccatc gcggcgtcga ggtcgagcgc cgcccaggcg atggcccggt
     1381 cgaagtcggc atcgccgctc cggagggaga cggccgagtc ggtgaccgcg gccgcgtgcg
     1441 ccacgcgggc gtcgtagagc gcgggcaccg aggcgagcac ccggtcgtag agggcgcgga
     1501 ccgagtcgcg cggcatcgcg ccgcccacga tgacgatcgg gatgccggag gcgtggacgt
     1561 ggaccagcct gccctcgggc tcgccgggac gcggcatcgg gacggcaccc tggtcgccga
     1621 ccgcgatgcg gagctggttg ggcgcctcgg gcacattgtg cgccggctgg ttggtggcgg
     1681 aggtggtgta gggactgccg accatcgcgc tgtactggcg ccggctctcc gcgaggacga
     1741 aggcgcgttc ggcatcgtcc caatagacat actggccccc gaccgccccg ggccacgcca
     1801 gctgcagatc cgactggaac tcggcgagga tctcgatcgg acgcaccgcg tcgacctcga
     1861 gcacgatgac cgccgccgga tcgtcctcgg cgacgaagac ccgctcgcgg acggtgaagg
     1921 ccggatgggt gtaggtgatc gtgaaaccgg cgggggtgat gtccacccgg cgcgccagct
     1981 cgctcgcggc gatcggctcg gcgtagaaag gcgtctggaa gcggagctgg aagccgtgca
     2041 ggagcttgag gggccaggcc cacgcctcga ggtggccgag ctcatcgccc atcacgatag
     2101 cgcggtgacc ggtggcgctc acgtaggtgc cggcgcgggc ggatccggtg aagtcgaggg
     2161 gaccggtccg ccagtggatg ggggtcggcg tggggagggc ggcgggtgcc tgggcaccca
     2221 tccgggcggc ggaaagggcc accagggtgg tcacagcgag ggaccgggac agggcacgtg
     2281 gcatatataa ggaaggtggc gggtggaatt gggccagcat tctctgtaac atgctgcaag
     2341 acaacgactt atgtcgatag ccctgggcgc ttggcggtcc ggacgtcgga tccggcgccg
     2401 ccgggaggcg ggggagtcct ccagcttgca catcgggcgc ggacgtgcaa gttacatccg
     2461 gtctgtacca cggcgcgata gcgtcgaaca cctggcggcg ggtcacgccg cagagcatcg
     2521 gaggatggaa cgatggccga gctgagcgat atggaaatgg ggtggtcagg cgccggctgg
     2581 atggatcccg cgccgatgcc cagcactccg gaaccggcac cgatggcccc gatgaccccg
     2641 gccgcgcccg ccccggcggc gccgcggtcg aagccggccg cgaagaagcc cgcccggaag
     2701 aaggcggcca agaagaagcc ggccaagaag aaggccgcca agaagcccag gaagaaggtg
     2761 gcgcggcgga agcccgccag gaaggcggcc aggaagcgcc ccgccaagcg gaaggccgcc
     2821 accagggcga agaagaaggg caagaagcgg cggtaagttg agacgccccg tcggaagtcc
     2881 ggcggggcgc ttcacttggc gcgccggtgc ccggttgcgg gacggcgcgg gggagtgtta
     2941 taacccgaca tgcgaaaagc gtccctcttc gtcctctccc tcgcgttggc ggggtgcggg
     3001 aagcccgtca aggaccggat cgcgctggtc ggcgtcaccg tgatcgacgg gaccgacgcc
     3061 ccggcgcggg tggaccaggt gatgatcatc cgcgggtcgc gcatcgacac gatcgcgccg
     3121 gcggcgggct tcgccatccc caagacggcg acggtgatcg acctcaaggg gaaatgggtc
     3181 atccccgggc tcatcgacgc cgatggcgac gtcgatcgct ggacggtcaa gcggtacctg
     3241 gccttcggcg tcaccgcggt gcgcgacgtg ggcaacgacc aggattcgat cctggcgctg
     3301 gccgaggggg cggcgctgca cagcctggtg agcccgcggc tctacgtgac cggcgcggcg
     3361 ctcgacggcg gcacaccatt cgaccccagc tcggcggcgc tgaccggcga gtcggacgcc
     3421 cgccgggcgg tggacgaccg ggtgcaggcc ggcgtgtcgt tcgtgtcgac gggaccgggg
     3481 ttcacgccgg tactcctgcg ggccgtggtg gacgaggcca ccaacctcaa cacgtcggtc
     3541 accgcccggc tgggcctcac cgatgcccgc accgccggca agatgggggt caagtcgctg
     3601 ctcgagctgt cgggcgtgcc ggaggcggtg gtgaaccccg agccgttcta cgccgcctac
     3661 cgcaaggcgc cgtggaacgg ctggaccacc accgagcgcg cctgggccgg gctcgactcg
     3721 gcgccgctcg gcgcagtggc caccgacctc gccaccgccg gcgtgacgct ggtgccggca
     3781 ctggtgctgc acgacacctg gagccggctc gatgattccg cagtgaccgc gtcgccggac
     3841 ctggcgtacg tgcctgcgac ggcgcgcagc gactgggaca tccccaagtt cgaggcgcag
     3901 gcgggatgga atgcctcgac ctacccggcg ttccgggcgg gacggagcaa gcaggatctc
     3961 ttcgtccgcg aattcaagct ggcgggggga cggatcgcgg cggggaccgg cgccgcgcgg
     4021 ctgatgctgg tcccgggagc cagcctgcac cgcgagctcg agctcctggt gaacgccggc
     4081 ctccccacgg atgacgccat ccgctccgcg acctcggtgg cggcggcgct gatcggcgcc
     4141 gactcgctgg ggaccctcga gccgggaaag gtggcggatc tcgtcgtgct caacgccgat
     4201 ccgctggccg atatccgcaa cacccggtcg atcgtcaagg tggtcctggg cggcctggtg
     4261 ctcgacgccg acagcctggc ccggcgcatc cgtcagtgac gctcacacgg ctggtgctgg
     4321 catggggcgt cggcgccgtg tggtacgcgc tggtcgacgc ggggctcggc cggtactggc
     4381 cggcggcggg cgtgaccttc cggccgaagg tgatcctggc ggagaccctg ctgctcacga
     4441 tgttcgcggc cctctggttc ggcagcctcg gccatggtgg ctggtggctc ctctttctgg
     4501 tcctgggcgc cctcatggag ggccccgtcc gcgcgcggca ccgcatcggc gccgcaccgg
     4561 gcggcgggcg tccctggctc gatgcggcca tcggtatatg ccgcgtcgtc atcgcaggag
     4621 gactgctctc atggagactc tcgtaacgcg gcacgccgcc gccatcgatc gcgccctcga
     4681 cgggctcacc cgctggtaca gcacccgccc caaccgcccc ggcatcccgg tccggcgcgc
     4741 gctgggcgtt accgcccccg atgacgacta caccgcccac cagatgg
//
LOCUS       DASUAP010000131         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_644231, whole genome shotgun sequence.
ACCESSION   DASUAP010000131 DASUAP010000000
VERSION     DASUAP010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644231"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VFU45_03110"
     CDS             complement(<1..1136)
                     /locus_tag="VFU45_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU5358085.1"
                     /translation="MSSPLAADLARIVGSRWVRERHAELATFSGDGLPTHRSEPKLVV
                     LPGSREELIAVIRLLHRLRVPFVARGAGTGLSGGALAGGDAVLVTLTRLDRILSLDPS
                     ARRATVEPGVVNARLSDAARPFRLQYAPDPSSQSACTLGGNVAENAGGPHCLKYGVTT
                     NHVTALEVILPDGSVTALGSPQGEAWGPDLVGLFVGSEGMFGIASAITLRLVPVPDAI
                     HTFLADFGSVRAAGEAVSAVIASGVIPAALEMMDQACVRAVEASIYAAGYPTDAAAVL
                     LVELDGRPATAAAESAAVHRILQGHGARSIREATDPLERARLWQGRKKAFGAMGRIAR
                     DLVVQDAVVPRSSLPDILDLIADVGRRYDLTISNVFHAGDGNLHP"
     gene            complement(1293..2186)
                     /locus_tag="VFU45_03115"
     CDS             complement(1293..2186)
                     /locus_tag="VFU45_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEU5358086.1"
                     /translation="MARARVTKARPFFRPVATAAFDQYLLDIQKLPMITDQAEERRLA
                     RLALNGDEKAAERLVTANLRFVISYVKKYQGHGLDLSELVAIGNEGLLKAVRKFDPDH
                     GVKFISYAVWWVRQAVLKALAEQTRSVRIPLNQNSALIKMSRAESFLSQELGREPTDQ
                     EIALALNDTVENIRASRRMTSAELSLDAPVERGDKGASTYGERFAGAGSNEIEETTDA
                     RLMREFIDRLFDRYLTPRERKILCLYYGLEQGTEALTLEKIGALLGVTRERIRQIRER
                     AFEKLRQSPDVQILKGSWVAA"
     gene            complement(2506..>2721)
                     /locus_tag="VFU45_03120"
     CDS             complement(2506..>2721)
                     /locus_tag="VFU45_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358087.1"
                     /translation="VTLSTFGLPTRAPAGLAAPAVRSAMATDKKNRRAELRFALVESV
                     GRAHRVGERWTIAVAPARVEEALAAIL"
BASE COUNT          442 a          914 c          957 g          408 t
ORIGIN      
        1 gggtggaggt tcccgtcgcc ggcgtggaag acgttgctga tcgtgaggtc gtatcgccgc
       61 cccacgtcgg cgatgaggtc gaggatgtcc gggaggctcg agcgcgggac gacggcgtcc
      121 tgcaccacca ggtcccgggc gatccggccc atcgcgccga aggccttctt ccgtccctgc
      181 cagagccggg cgcgttcgag cgggtcggtg gcctcgcgga tggagcgcgc gccgtggccc
      241 tggaggatcc gatgcaccgc ggccgattcc gccgccgcgg tcgcgggccg cccgtccaat
      301 tccacgagca ggacggcggc ggcgtcggtg gggtagccgg ccgcgtagat cgaggcctcg
      361 accgcgcgca cgcaggcctg gtccatcatc tcgagcgccg cagggatgac gcccgaggcg
      421 atgaccgcgc tcaccgcctc gcccgcggcg cggacgctcc cgaagtccgc caggaaggtg
      481 tggatcgcgt cggggaccgg gaccagccgc agcgtgatgg ccgacgcgat gccgaacatc
      541 ccctcgctgc cgacgaagag ccccacgaga tcggggcccc acgcctcccc ctggggtgag
      601 ccgagcgccg tcacgctccc gtcggggagg atcacctcga gcgcagtgac atggttggtc
      661 gtgacgccgt acttgaggca gtgcgggccg ccggcgttct cggccacatt gccgcccagg
      721 gtgcaggcgg actggctcga aggatcgggc gcatactgca ggcggaaggg acgggccgcg
      781 tccgacagcc gggcattcac cacgcccggc tcgaccgtgg cgcgccgggc cgacggatcg
      841 agggagagga tccggtcgag tcgggtgagc gtcacgagca ccgcgtcgcc gcccgccagc
      901 gcaccaccgg agaggccggt cccggcgccg cgcgccacga acggcacacg gagccgatgg
      961 aggagccgga tgacggcgat cagctcctcc cggctccccg gcagcacgac gagcttcggt
     1021 tccgagcggt gggtgggcaa gccatcgccg ctgaaggtgg cgagctcggc gtggcgctcc
     1081 cgcacccatc gtgaccccac gatgcgggcc aggtcggcgg cgagcgggga ggacatgggg
     1141 ggaatgtagg agagctatca gcgatcagct atccgctatc agccgccccg aacgcgaacg
     1201 agcctccgac gaccgaatcg gaggctcttc cgtgacccga gcaatctgat agctgatagc
     1261 cgatagctga tagctgtcag cccctcaaac cctcacgccg ccacccagct ccccttcagg
     1321 atctggacgt cggggctctg gcggagcttc tcgaaggcgc gttcgcggat ctggcggatc
     1381 cgctcccggg tgacgccgag gagggcaccg atcttctcga gggtgagcgc ctcggtgccc
     1441 tgttccaggc cgtagtagag gcagaggatc ttgcgctcgc gcggggtgag gtaccggtcg
     1501 aacagccggt cgatgaactc acgcatcagc cgggcgtcgg tggtctcctc gatctcgttc
     1561 gaccccgcgc cggcgaaccg ctcgccgtag gtgctggcgc ccttgtcgcc ccgttccacc
     1621 ggcgcgtcga gcgaaagctc ggccgaagtc atccggcggc tggcgcggat gttctccacc
     1681 gtgtcgttga gcgccagcgc gatctcctgg tcggtcggtt cccggcccag ctcctgggag
     1741 aggaaactct cggcgcggga catcttgatc agcgcgctgt tctggttgag cgggatccgc
     1801 acgctccgcg tctgctcggc cagcgccttg agcaccgcct ggcgcaccca ccacaccgca
     1861 tacgaaatga acttgacccc gtgatcggga tcgaacttgc ggaccgcctt gaggaggccc
     1921 tcgttcccga tcgcgaccag ctccgacagg tcgagcccgt ggccctgata cttcttcacg
     1981 taggagatga cgaaccggag gttggccgtc accagccgct cggccgcctt ctcgtcgcca
     2041 ttcaaggcca accgggccag gcggcgctcc tcggcctggt cggtgatcat cggcagcttc
     2101 tggatgtcga gcaggtactg gtcgaacgcc gccgtggcga cggggcgaaa gaacggacgc
     2161 gccttcgtga ctcgggcacg ggccatagct accccacggg ctggaggagc atcactgctc
     2221 ggattgcacg gctgaaggat cgggacctgc ggccgaccgg tcggtcggga gagggaagcc
     2281 aacataggga attcgcaaag aacggtcaag catctcaaaa tttccacatc acaaagtgtt
     2341 cacctgaaag ggcttcccgt ctttgtccac gccggctcgc cgccggcgga agcattcccg
     2401 gtcggagctg acagtacaag tcgtttactg acaatgagtt acggactgtg ttcgaggtgg
     2461 aacgatgtgg aaggaatgtg gtggaaggcg gggaaagcgt cggcgtcaga ggatggctgc
     2521 gagcgcctcc tcgacccgag ccggggccac cgcgatggtc caccgctccc cgacacggtg
     2581 cgcccgcccg accgattcga cgagtgcgaa tcggagctcc gcccgccggt tcttcttgtc
     2641 ggtggccatc gccgaacgca cggcaggagc cgccaggccg gccggggcgc gggtcggcag
     2701 gccgaacgtc gacagcgtga c
//
LOCUS       DASUAP010000132         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_644239, whole genome shotgun sequence.
ACCESSION   DASUAP010000132 DASUAP010000000
VERSION     DASUAP010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644239"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1090)
                     /locus_tag="VFU45_03125"
     CDS             complement(212..1090)
                     /locus_tag="VFU45_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU5358088.1"
                     /translation="MGSLVIVAIGLFIGTGILFGGGHLSRARDVQVDFHEVSGLKPGS
                     PVRVSGVGVGRVERIDLEGVGKVRVTFSVPPRIAPRIDAQASVLAVGLVGDVALDYNP
                     GSSPMMLAEGQVLTGTEAAPGLSAKLEDMGAQAKQVMAGAAEILNRRTADDLHNTLLA
                     MQKLMNTYSDPHQGPSAELSATMVSLRKMSDQLDHLLSDPHTANTLAHLDTLSGNLAA
                     STRTLGDLSTELTRTSASLDTLLAGMNAGKGTLGKLATDTGLYNDARRTSAALTALLD
                     EIRKNPGKLNIQVKMF"
     gene            complement(1130..1882)
                     /locus_tag="VFU45_03130"
     CDS             complement(1130..1882)
                     /locus_tag="VFU45_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5358089.1"
                     /translation="MIQLTGVSKRFGEQVILDGVDFDVRDGETVALLGPSGTGKSVLL
                     KHINGLIHPDAGRIMVDGKDVGTLRGKALAELRARIGYVFQNGALFDSMNVFENVRLG
                     ITDDAKYRDLAYSRERVAKCLQLVNLTPETAEKFPAQLSGGMRKRVGIARAIAGESKY
                     LLYDEPTSGLDPVNADIIDALIGRLGDELGVTSVMVTHDVRGAFRVADRLALLTDGKI
                     VLQGTPQEFLASEHPKVKAFLERDLDTSTFAA"
     gene            complement(1879..2670)
                     /locus_tag="VFU45_03135"
     CDS             complement(1879..2670)
                     /locus_tag="VFU45_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5358090.1"
                     /translation="MAAATLDIRRWIGRAALTSAAHTGRVSLLLAEIFKGLREWRIWL
                     PRAMTEAWNIGVGSLFIVLLVSSFAGAVTAFQTGYQFKSSLPIYVVGTLVVSSIVLEL
                     GPVMTGLILAGRIGARYAAELGTMRVTEQIDALESLGRSPVSHLIIPRVVAGMIMIPA
                     LTVVSWVVGILAGWRGALAALPQLTNDDFMYGAALYWRPFDAWYALIKSVFFAAAITI
                     IPCYVGFNTQQGAEGVGKSTTAAVVSTSVIILLLDAVLAKVLLNP"
     gene            3070..3840
                     /locus_tag="VFU45_03140"
     CDS             3070..3840
                     /locus_tag="VFU45_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358091.1"
                     /translation="MTRYSPENASAFRRKLAAWFRKHGRDLPWRRTRDGYRIVVSEFM
                     LQQTQVSRVLEYYPRFLATYPTVEHLARARPAAVRESWDGLGYYRRAANLHALAKAVV
                     RDHAGKLPADPAMLRSLPGVGPYTAGAVASFAYERAEPAVDTNVARVIRRAFHPRRAA
                     DPNDRVVWATARTLLPRTGKAAWTFNQAIMELGALICTARVAKCGECPVRGACKTGTS
                     QLERTTGTRQTEPRRARRSTKRHGEQLCDVASRAGLRL"
BASE COUNT          614 a         1336 c         1344 g          604 t
ORIGIN      
        1 gcggagtcat cctgagcgaa gcgaaggatc tcaacccgat cctcgccttg caggcgggcc
       61 cgccacgctc ccggctagtt cagcttcggc ttgagatcct tcggtcgctt cgctccctca
      121 ggatgactcc gctgcaattc cgcacttcgc gcttggccac ccgccctatc cgcctcagtt
      181 cacccgccct gtccgccttc gtcgaccggg tctagaacat cttcacctgg atattcagct
      241 tccccgggtt cttccggatc tcgtcgagca gcgcggtgag cgcggcactg gtgcggcggg
      301 cgtcgttgta gagcccggtg tcggtggcga gcttgcccag ggtccccttc ccggcgttca
      361 tcccggccag gagcgtatcg agcgaggcgc tggtgcgggt cagctcggtg ctgaggtcgc
      421 cgagggtgcg ggtcgaggcc gccaggttcc cggagagcgt gtcgaggtgg gccagggtgt
      481 tggcggtgtg cgggtcggag agcaggtggt cgagctggtc gctcatcttc cggaggctca
      541 ccatcgtggc cgagagctcg gccgagggcc cctggtgcgg gtcgctgtag gtgttcatca
      601 gcttctgcat cgcgagcagg gtgttgtgca ggtcgtcggc ggtgcgacgg ttgaggatct
      661 cggcggcgcc cgccatcacc tgcttggcct gggcgcccat gtcctcgagc ttggccgaga
      721 ggcccggcgc cgcctcggta ccggtgagca cctggccttc ggccagcatc atcggggagc
      781 tgcccggatt gtagtcgagg gccacgtcgc ccacgaggcc gacggcgagg accgacgcct
      841 gcgcatcgat ccgcggggcg atccgaggcg gcaccgagaa ggtcacccgc accttcccca
      901 ccccttcgag gtcgatccgt tcgacccgcc ccacgcccac gccggagacc cgcaccgggc
      961 tcccgggctt gagcccgctc acctcgtgga agtcgacctg gacgtcgcgg gcgcggctca
     1021 ggtggccgcc accgaagagg atcccggtgc cgatgaagag accgatggcg acgatgacca
     1081 gcgatcccac ggtgacttca cgcctgtagt gcagatccat tggggcccgc tatgccgcga
     1141 aagtggaagt gtcgaggtcg cgctcgagga acgccttcac cttcgggtgc tccgaggcca
     1201 ggaactcctg cggcgtgccc tggagcacga tcttcccgtc ggtgaggagc gcgagccggt
     1261 cggcgacgcg gaaggcgccg cgcacgtcgt gggtcaccat cacgctggtg acgccgagct
     1321 cgtcccccag gcgcccgatc agcgcgtcga tgatgtcggc gttgaccggg tcgaggcccg
     1381 aggtcggctc gtcgtagagg aggtacttgg actcccccgc gatggcccgc gcgatgccga
     1441 cccgcttgcg catcccgccg gagagctgtg ccgggaactt ctccgcggtc tcgggagtga
     1501 ggttcaccag ctgcaggcac ttggcgaccc gctcccggga gtacgccagg tcccggtact
     1561 tcgcatcgtc ggtgatgcca agcctcacgt tctcgaagac attcatcgag tcgaacaggg
     1621 cgccgttctg gaagacgtag ccgatccggg cccgcagctc ggcgagcgcc ttgccccgca
     1681 gcgttccgac gtccttgcca tccaccatga tgcgccctgc gtcgggatgg atgagcccgt
     1741 tgatgtgctt gaggagcacg ctcttcccgg tgcccgaggg ccccagcagc gccacggtct
     1801 cgccatcccg cacgtcgaag tcgacgccat cgaggatgac ctgctcgccg aagcgcttcg
     1861 agacgccggt cagctggatc acgggttgag cagcaccttg gcgagcacgg cgtcgaggag
     1921 caggatgatg accgaggtgc tcacgaccgc cgccgtcgtc gacttgccga ccccttcggc
     1981 gccctgctgg gtgttgaagc cgacgtagca ggggatgatc gtgatcgccg cggcgaagaa
     2041 gaccgacttg atcagggcgt accaggcgtc gaacgggcgc cagtagagcg cggcgccgta
     2101 catgaagtcg tcgttggtga gctgcggcag cgccgccagg gcgccgcgcc acccggccag
     2161 gatcccgacc acccacgaca ccacggtgag tgcggggatc atgatcatcc ctgccaccac
     2221 ccgcgggatg atgaggtggg acaccggcga gcgccccagg ctctccagcg cgtcgatctg
     2281 ctcggtgacg cgcatcgtgc cgagctcggc ggcgtaacgc gcgccgatcc ggccggccag
     2341 gatgaggccc gtcatcaccg gtcccagttc gaggacgatg gacgacacga cgagcgtgcc
     2401 caccacgtag atcggcaggc tcgacttgaa ctggtacccg gtctggaatg ccgtcacggc
     2461 gccggcgaac gacgacacca ggagcacgat gaagaggctc ccgacgccga tgttccaggc
     2521 ctccgtcatg gcgcgcggga gccagatccg ccattccctg agacccttga agatctcggc
     2581 caggagcagg ctgacccggc cggtatgggc ggcggaggtc agggcggcgc gtccgatcca
     2641 gcggcggatg tccagggtgg cggcggccat cgggcctcga gctcagggat gcggcgccgg
     2701 tcccgggccc cgccggcggt cgcgtaagcc attagtttgt cagcacatga cggctaagtc
     2761 cacccccgcc ggctccctgg gtccaccgcc ggaacgtggg accacggaga gcacggagga
     2821 cacggagagg cacggagaac tgccgagcag tgtggtcgac cggaccgatt ccgctgccgt
     2881 ggcgaaatct cgcccggaac aggctccgga gcacgatgcg gtacgaagcc ccgcccttcg
     2941 gtgcggcaag gtgcacggag tcccgttcgc gaggccacaa ctactgcagt cctccgtgaa
     3001 tctccgtgtc cctccgtgcc cgagcctgcc ctgagcgcag cgaagggtgg tcccacaaac
     3061 cgaacgccaa tgacccgata ctcacctgag aatgcatccg ccttccgccg aaagctggcg
     3121 gcctggttcc ggaagcacgg acgcgacctg ccctggcggc gcacccggga cgggtaccgc
     3181 atcgtggtgt cggagttcat gctgcagcag acgcaggtga gccgggtgct ggagtactac
     3241 cctcgcttcc tcgccaccta tcccaccgtg gagcacctcg cgcgggcccg ccccgccgcc
     3301 gtgcgcgaga gctgggacgg cctcggctac taccgccgcg ccgccaacct gcacgccctg
     3361 gccaaggcgg tggtgcgcga ccacgccggg aagctccccg ccgacccggc catgctccgc
     3421 tcgctgccgg gggtcggccc ctacaccgcc ggtgccgtgg cgagcttcgc gtacgagcgg
     3481 gccgagccgg cggtggacac caacgtggcg cgagtcatcc ggcgggcgtt ccatccccgg
     3541 cgcgccgccg acccgaatga ccgggtggtg tgggccaccg cgcggacact cctcccccgc
     3601 accggcaagg ccgcgtggac cttcaaccag gcgatcatgg agctgggggc cctgatctgc
     3661 accgcgcggg tggcgaagtg cggggagtgt ccggtgcggg gagcgtgcaa gacggggacg
     3721 tcgcaattgg aacgaaccac cggcacaaga cagactgaac cacgaagggc acgaaggagc
     3781 acgaagaggc acggagaaca actttgtgat gtggcctccc gggcgggact ccggctttga
     3841 gaccacattg actgcaggtc tccgtgtgtc tccgtgcagc tccgtgccct ccgtggtc
//
LOCUS       DASUAP010000133         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_330510, whole genome shotgun sequence.
ACCESSION   DASUAP010000133 DASUAP010000000
VERSION     DASUAP010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330510"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..2603
                     /locus_tag="VFU45_03145"
     CDS             294..2603
                     /locus_tag="VFU45_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU5358092.1"
                     /translation="MSASSDAEMLASLNPAQREAVEHVTGPALVVAGAGSGKTRVLTL
                     RIARLIDEHGVPPERILAVTFTNKAAGEMKGRVGRLLERDPAGLWIGTFHSISARLLR
                     REAEHLGFTRQFTIYDEDDRNSLIGRILDQLGHDKKKFTPRGVAGIISGAKNRMSEPS
                     ELKASAPFDRFVQVAAEVMEALGPALRAQNAMDFDDLLLHPVRLFHEHPDRLAAWQER
                     FSFILVDEFQDTNAAQYRLVRFLGKGGNVFAVGDDDQSIYGWRGADVRNMQEFQRDFP
                     GTRVIRLEENYRSTPSVLEAANAVIAENKGRIGKTLRTTRRGGEPVTIVAAADERDEA
                     EWVVKEFGARRADWDWNAMAVLYRTNAQSRAFEEALRRSGTPYRIVGAISFYDRREVK
                     DLLAYLRLVANPADDEAFLRAVNVPRRGIGDTSLAQVETVARQWKKSLLETARAADRI
                     PDLRPNVRNALMGFAEQVDRLMASAAIQAPAQVLESLIDAIGYEKHLLDEGPEGADRW
                     ENVRELVAGAAEWSEEVEEGAEGTPLERFLEEAALLSSHDKISGTEEGVTLMTLHTAK
                     GLEWPVVVMAGMEDGLFPLARAEETPDGVEEERRLAYVGITRARDKLFLTWARSRRRG
                     GQLLPGIQSRFLRALPPGTIEEKRTSALGSYGGWANGRAGAWSGGRTDASKARPSGGF
                     GWLDTSASDRPTVRPSAPAPAENDDAPRYVKGERVKHRKFGSGVIQGLTGVGRDLKVT
                     VRFDDEETGVKQLLVAYAGLERDRDWESA"
     gene            2600..2902
                     /locus_tag="VFU45_03150"
     CDS             2600..2902
                     /locus_tag="VFU45_03150"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU5358093.1"
                     /translation="MTIDKQAIHHIAALAELAVTDGEAVALASQLERIVGMVAQLQEA
                     DVPAEAQEYVAGPDRVTLRDDVVAPEKLHRTPEMLTTGFQHGLYVVPRLGSQEHAE"
     gene            2899..>3657
                     /locus_tag="VFU45_03155"
     CDS             2899..>3657
                     /locus_tag="VFU45_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU5358094.1"
                     /translation="MTAVKNISDAVRTGRTTAARSAEHVTRLLIDSDSKGLNAALDWS
                     PEYVAREARRIDHHSVKGRLAGVPVAVKDNIATLDLPTTCASRILDGYVSPFEATVVK
                     RLREAGAYVACKANLDEFAMGSSTEWSAFGRTRHPLDHYRVPGGSSGGSAALVAAGVT
                     PVALGSETGGSVRQPASFCGVVGVKPTYGRVSRHGLVAFGSSLDCVSPFGEKVDDAAR
                     LLAVISGHDPLDATTADRPPLTLDEPRRDLKGLTI"
BASE COUNT          580 a         1326 c         1271 g          480 t
ORIGIN      
        1 tctgcgtggg gatcgcctgc ggtgacccga gtctcacccc atcggggtca tcctgaggcc
       61 cgcagggccg aaggatctca actcgatgcg cgcagcgtcg ggcccgctgc gtgctgacgc
      121 cggagcccgg caccggttcg agatccttcg gggccccacg acccctcagg atgactccga
      181 tgcagctcaa gcccaccgcg cttgccaggg catcagtgaa caagaccggg tcggggtgac
      241 gcctgcgcga aaatgatgcc cggtcatcgc cgaagtgcga ttatcttccc gccgtgtccg
      301 cctcctccga cgccgaaatg ctcgcctccc tgaaccccgc ccagcgggag gcggtggagc
      361 acgtcaccgg ccccgctctg gtcgtcgcag gcgcggggtc agggaagacc cgcgtcctca
      421 ccctccgcat cgcgcggctc atcgatgagc acggcgtccc gccggagcgg atcctcgcgg
      481 tcaccttcac caacaaggcc gccggcgaga tgaagggccg cgtcggccgg ctcctcgagc
      541 gcgacccggc ggggctctgg atcggcacct tccactcgat ctccgcccgg ctcctgcggc
      601 gcgaggcgga gcacctgggc ttcacccgcc agttcacgat ctacgacgag gacgaccgca
      661 actcgctcat cggccgtatc ctcgaccagc tgggccacga caagaagaag ttcaccccgc
      721 gcggcgtcgc cgggatcatc tccggcgcca agaaccggat gagcgagccg tccgagctca
      781 aggcctccgc ccccttcgac cgcttcgtcc aggtggccgc ggaggtcatg gaggcgctcg
      841 gccccgcgct ccgggcgcag aacgcgatgg acttcgacga cctcctgctc catccggtgc
      901 ggctcttcca cgaacacccc gaccggctgg ccgcctggca ggaacgcttc tcgttcatcc
      961 tggtcgacga gttccaggac accaacgccg cccagtaccg cctggtgcgg ttcctgggca
     1021 agggcgggaa cgtctttgcc gtcggcgacg acgaccagtc gatctacggc tggcgcgggg
     1081 ccgacgtgcg caacatgcag gagttccagc gcgacttccc cggcacccgg gtcatccggc
     1141 tggaggagaa ctaccgctcc acgccctcgg tgctcgaggc cgccaacgcg gtcatcgccg
     1201 agaacaaggg ccggatcggg aagacgctcc gcaccacccg gcggggcggg gagccggtca
     1261 cgatcgtggc cgccgccgat gagcgcgacg aggcggagtg ggtggtcaag gagttcggcg
     1321 cccgccgggc cgactgggac tggaacgcga tggcggtgct ctaccgcacc aacgcgcaga
     1381 gccgcgcgtt cgaggaggcg ctccgccgct cgggcacgcc ctaccggatc gtcggcgcca
     1441 tcagcttcta cgaccgccgc gaggtcaagg acctgctggc gtacctccgc ctggtggcca
     1501 acccggcgga cgatgaggcg ttcctccgcg cggtcaacgt cccccggcgc ggcatcggcg
     1561 acacgagcct ggcccaggtg gagacggtgg cgcgccagtg gaagaagtcc ctgctcgaga
     1621 ccgcccgcgc cgccgaccgg atccccgacc ttcggcccaa cgtccgcaac gcactgatgg
     1681 gtttcgccga gcaggtggac cggctgatgg cgagcgccgc catccaggcg ccggcgcagg
     1741 tgctcgaatc gctcatcgac gccatcggct acgagaagca cctcctcgac gaaggccccg
     1801 agggcgccga ccggtgggag aacgtccgtg aactcgtggc cggcgccgcc gaatggtcgg
     1861 aagaggtgga ggaaggtgcc gaagggacgc cgctcgaacg cttcctcgag gaggcggcgc
     1921 tcctctcgag ccacgacaag atcagcggca ccgaggaagg cgtcacgctg atgaccctcc
     1981 acaccgccaa ggggctcgag tggccggtgg tggtcatggc ggggatggag gacggactct
     2041 tcccgctggc gcgcgccgag gagacccccg acggcgtcga ggaggaacgg cgactggcgt
     2101 acgtcggaat cacccgggcg cgggataaac tcttccttac ctgggcccgc tctcggcggc
     2161 gcggcggcca gctcctgccg gggatccaga gccgcttcct ccgggccctc ccgccaggca
     2221 ctatagagga gaagcggacc agcgcgctcg ggagttacgg cgggtgggcc aatgggcgcg
     2281 cgggcgcgtg gtcaggtggg cggacggacg cgtcgaaggc gcggccgtca gggggcttcg
     2341 gctggctcga caccagcgcg tccgaccgtc cgaccgtgcg tccatccgcc cccgcgccgg
     2401 cggagaacga cgacgccccc cgctatgtca agggcgagcg ggtcaagcac cggaagttcg
     2461 ggagcggggt catccagggg ctgacggggg taggacggga cctcaaggtg acggtccggt
     2521 tcgacgacga agagacgggc gtcaagcagc tgctcgtggc ctacgccggc ctcgagcgcg
     2581 atcgcgactg ggagagcgca tgaccatcga caagcaggcc atccaccaca tcgccgcgct
     2641 cgccgagctc gcggtcaccg acggcgaggc ggtggcgctg gcgtcgcagc tggaacggat
     2701 cgtcgggatg gtggcccagc tccaggaggc cgatgtcccc gccgaggccc aggaatacgt
     2761 ggccggcccc gacagggtga cgctccgcga cgacgtggtg gccccggaaa agctccaccg
     2821 caccccggag atgctcacca ccggcttcca gcacggcctc tacgtggtgc cgcgcctggg
     2881 ctcccaggag cacgccgaat gacggcggtc aagaacatca gcgatgcggt ccgcaccggc
     2941 cgcaccacag cggcgcgctc ggcggagcac gtcacccggc tcctgatcga ctccgacagc
     3001 aagggcctca acgccgccct cgactggagc cccgagtacg tggcccgcga ggcgcggcgc
     3061 atcgaccacc actcggtcaa gggacggctc gccggggtgc cagtggcggt caaggacaat
     3121 attgccaccc tcgacctccc cacgacctgc gcctcccgga tcctcgacgg ctacgtgagc
     3181 cccttcgagg ccacggtggt caagcggctc cgcgaggccg gcgcctacgt agcctgcaag
     3241 gcgaacctcg acgaattcgc gatggggagc tccaccgaat ggtcggcgtt cgggcggacc
     3301 cggcatccgc tcgaccacta tcgcgtgccc ggcggctcct ccggcggctc ggcggcgctg
     3361 gtggcggcgg gcgtcacccc ggtggcgctc ggctccgaga ccggcggctc cgtccgccag
     3421 ccggcgagct tctgcggtgt ggtgggcgtc aagccgacct atggccgggt gagccgccac
     3481 gggctcgtcg ccttcggctc ctcgctcgac tgcgtgagcc ccttcggcga gaaggtcgac
     3541 gacgcggcgc ggctgctggc cgtcatcagc ggccacgacc cgctcgacgc caccaccgcc
     3601 gaccggccgc cgctcaccct cgacgagccg cgccgggact tgaagggcct cacgatc
//
LOCUS       DASUAP010000134         5737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_998498, whole genome shotgun sequence.
ACCESSION   DASUAP010000134 DASUAP010000000
VERSION     DASUAP010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998498"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..455
                     /locus_tag="VFU45_03160"
     CDS             24..455
                     /locus_tag="VFU45_03160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5358095.1"
                     /translation="MPARLRPATTDDAPAIAGLLTQLGYPTLVDAARARLADLLRRGP
                     PDRVVVAEVDGKVVAVMTLHLTPELHRARPIGRVTALVVDESVRGSGVGAEMMEEAER
                     ILRADGAGLLELTSNMRRVDAHRFYERLGYQKTSFRFAKEL"
     gene            complement(433..1746)
                     /gene="hemL"
                     /locus_tag="VFU45_03165"
     CDS             complement(433..1746)
                     /gene="hemL"
                     /locus_tag="VFU45_03165"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU5358096.1"
                     /translation="MTATPRDTESTRLFAAAREVMPGGVSSPVRAFRGVGGTPRFVME
                     GQGARIVDADGNRYVDYVMSWGALALGHAHPAVVAAITRQAARGTSFGAPTVLETALA
                     ERIISALPAVEMVRFVSSGTEAAMSALRLARAATGRAKIVKFTGCYHGHADALLVQAG
                     SGVATLSLPDSPGVPRSAVADTLTAPFNDAAAVAALFDRYPGEIAAVTVEPIAGNMGM
                     VLPAPGFLKALRDLTRKHGALLIFDEVMTGFRVAWGGAQRIYGIDPDLTCLGKVIGGG
                     LPVAAYGGRRDLMEELAPAGKVYQAGTLSGNPLAMAAGIATLDELSRPGTYDRLGALS
                     AAVVEIVRSAAEQAGVAVQTAAVGGMWGFFLNARPVTNYDSARTSDTAGFSRCFHALL
                     EHGVYIAPSAFEAGFVSTMHDDAALEQTRAAFAHAMAAVTAPSRT"
     gene            complement(1743..2732)
                     /gene="hemB"
                     /locus_tag="VFU45_03170"
     CDS             complement(1743..2732)
                     /gene="hemB"
                     /locus_tag="VFU45_03170"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU5358097.1"
                     /translation="MSSSPSSVQMRRLRRSAAMRGLVRETRLSPAQFVLPLFVTEGKN
                     VERPIAPMPGHSQWSVDRITAQVDRAVALGVQSVLLFGIPDVKDAVGSGAADPKGPVA
                     RALAAIKKHAPGVVVIADVCLCEYTDHGHCGIVSPQGEVKNAETLPLLAGAAVAYADA
                     GADIVAPSAMMDGQVAAIRAGLDGKEHGQVAVMAYAAKSASAFYGPFRVAAGSTPAFG
                     DRRAYQMDPAKAREALREVDLDIEEGADLVMVKPAGPCLDIIRRVRERTDLPLAAYQV
                     SGEYAMLKAAAERGWLDERRAAMESLVAIARAGADIIITYHALDAARWIREEG"
     gene            complement(2740..3555)
                     /locus_tag="VFU45_03175"
     CDS             complement(2740..3555)
                     /locus_tag="VFU45_03175"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEU5358098.1"
                     /translation="MSRGPESGALSGRTVVLTRSREQSRALTELLESRGATVIVVPMI
                     QFVPAADPAPLDAALHSLTSFDIIAFTSVNAVAATFGRMAALGIGPGALDDVTIAAVG
                     DATATALREQGVTPTAQPARATGADLAAALGAVAGRRILLPRSDLARSDLPEALRRAG
                     AEVVDVVAYHTLPDQAGGGLARVLLERPVDAIAFASPSAVDIAIDALERAGSSGLGGD
                     AAPAIVAIGPVTARQVETRGLKVAAVALQHDAVGLADAVVAALGARTAVPRTP"
     gene            complement(3539..4489)
                     /gene="hemC"
                     /locus_tag="VFU45_03180"
     CDS             complement(3539..4489)
                     /gene="hemC"
                     /locus_tag="VFU45_03180"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU5358099.1"
                     /translation="MSAPLKKLTIGTRGSPLALVQTDLTALALRGAWPGLEVDTLRIT
                     TKGDILLDRPLSTLGDKGLFVAEIESALRDGRADLAVHSAKDLPSELPADMRIAAFAR
                     RADPRDALVSKFDGVAALPVGARVGTSSPRRSAQLKALRPDIELLDVRGNVGTRLRKL
                     ADGGYDALLLAAAGLERLGVRSGADGATIHFLPVETMLPAPGQGALAIEVNAARPEVV
                     AMVRALNDPDTEAAVLAERAFLGAVGGGCSSAVAAHARVQGTALSLHAMIGAVDGRVV
                     RGSTKGMTGEGPALAAALASKLLAEGGAELLRGAADVTRA"
     gene            complement(4486..5556)
                     /gene="hemE"
                     /locus_tag="VFU45_03185"
     CDS             complement(4486..5556)
                     /gene="hemE"
                     /locus_tag="VFU45_03185"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEU5358100.1"
                     /translation="MNVRDTLFCRAARREPVERTPVWFMRQAGRVLPEYRAVREGRTL
                     LEITQVPELCAEVTLQPVARFDVDAAVLFADIVSPLVAIGVDVTLVDDVGPVVGHPIR
                     ANADLEALRPIEPGQDVPHVLETIRIVDRELGGRIPVIGFAGAPFTLAAYLVEGRPSR
                     DFRHTKAMMYSAPALWHALMERLTAIIIPYLRAQAEAGAGALQLFDSWVGALSPQDYA
                     EFVQPYARRIFGELRAAGVPLIHFGTNTATLLDAMKTDGAGVIGVDWRIPLDRAWAQV
                     GHSLGIQGNLDPMALCGSPEHLKGAIADVLRRAGGRPGHIFNLGHGLHPATPVEQVQR
                     TVEWVRELGARADAPLASAAAR"
     gene            complement(5553..>5737)
                     /locus_tag="VFU45_03190"
     CDS             complement(5553..>5737)
                     /locus_tag="VFU45_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358101.1"
                     /translation="SELEQAALRQMAARIVNKLMHGPLTALNEAPEPEIAAAVERLFG
                     VTGGPRAERAASGIRM"
BASE COUNT          769 a         2129 c         2101 g          738 t
ORIGIN      
        1 ctttcgctga tagctttcac tccatgccag cgcgcctccg ccccgccacc accgacgacg
       61 cccccgcgat cgccgggctc ctgacgcaac tcggctaccc caccctggtc gacgcagcgc
      121 gcgcccggct ggccgacctc ctccgccgcg gcccgccgga tcgcgtcgtg gtggcagagg
      181 tggacggcaa ggtcgtcgcg gtgatgaccc tccacctgac cccggagctc caccgcgcac
      241 ggccgatcgg ccgggtcacg gcgctggtgg tggacgagtc ggtgcggggg agcggggtcg
      301 gggcggagat gatggaggag gccgagcgga tcctgcgggc ggacggcgcc gggctcctcg
      361 aactcacgag caacatgcgc cgggtcgacg cccatcggtt ctatgagcgc ctcggctacc
      421 agaagacctc gttcaggttc gcgaaggagc tgtgacggcg gccatcgcgt gcgcgaacgc
      481 cgcgcgcgtc tgctccagcg ccgcgtcgtc atgcatcgtt gagacgaatc ccgcctcgaa
      541 cgccgacggg gcgatgtaca ccccgtgctc gaggagcgcg tggaagcagc gggagaaccc
      601 cgccgtgtcg ctggtgcggg ccgagtcgta gttcgtgacc ggcctggcgt tgaggaagaa
      661 cccccacatc ccccccaccg cagcggtctg caccgcgacc ccggcctgtt ccgccgccga
      721 ccggacgatc tccaccaccg ccgctgagag ggcacccagc cggtcgtagg tgccggggcg
      781 cgacagctcg tcgagtgtgg cgatccccgc cgccatcgcg agcgggttcc ctgagagcgt
      841 ccccgcctgg tacaccttcc ccgccggcgc cagctcttcc atcaggtcgc gccggccgcc
      901 gtacgccgcc accgggagcc cgccgccgat caccttgccg aggcaggtca ggtcggggtc
      961 gatcccgtag atccgctgcg cgccacccca ggctacccgg aagcccgtca tcacctcgtc
     1021 gaagatgagg agggcgccgt gcttccgcgt gaggtcgcgt agcgccttga ggaagccggg
     1081 cgccgggagc accatcccca tgttccctgc gatcggttcg accgtcaccg cggcgatctc
     1141 gccgggatac cggtcgaaga gcgccgccac cgccgcggca tcgttgaacg gcgcggtcag
     1201 cgtatcggcc acggcgctcc ggggcacccc cggactatcc ggcagcgaga gcgtggccac
     1261 gcccgacccc gcctgcacca gcagggcgtc ggcgtgcccg tggtagcatc cggtgaactt
     1321 gacgatcttg gcgcgcccgg tggcggcgcg cgccagccgg agcgcgctca tcgccgcctc
     1381 ggtgccgctc gagacgaatc gcaccatctc gaccgcgggc agggcggaga tgatccgctc
     1441 cgccagcgcg gtctcgagca cggtaggcgc gccgaacgac gtgccccgcg ccgcctgccg
     1501 ggtgatggcc gccaccaccg ccgggtgcgc gtgccccagc gccagcgccc cccacgacat
     1561 cacgtagtcc acgtaccggt tgccgtcggc gtcgacgatg cgggctccct gcccctccat
     1621 cacgaagcgc ggggtcccgc cgacgccgcg gaaggcgcgg accgggctgc tcaccccacc
     1681 cggcatcacc tcccgcgcgg cggcaaagag gcgggtcgac tcggtgtcac gcggggtcgc
     1741 ggtcaaccct cctcccggat ccagcgcgcg gcgtcgagcg cgtgatacgt gatgatgatg
     1801 tcggcgccgg cgcgggcgat ggcgacgagg ctctccatcg ccgcgcgccg ctcgtcgagc
     1861 cacccccgct ccgccgcggc cttgagcatc gcgtattcgc cgctcacctg gtacgcggcg
     1921 agcggcaggt cggtccgctc ccgcacccgg cggatgatgt cgaggcaggg gcccgccggc
     1981 ttcaccatca ccaggtcggc cccctcctcg atgtcgaggt ccacctcgcg gagcgcctcc
     2041 cgcgccttgg cgggatccat ctggtaggcg cgccggtcgc cgaaggcggg ggtcgagccg
     2101 gcggccaccc ggaacgggcc gtagaacgcc gacgccgact tggcggcgta cgccatcacc
     2161 gccacctggc cgtgctcctt gccgtcgagc ccggcgcgga tcgccgccac ctggccgtcc
     2221 atcatcgcgc tcggcgccac gatgtcggcg ccggcatcgg cgtaggccac cgccgcaccg
     2281 gccagcagcg gcagcgtctc ggcgttcttc acctcgccct gcggcgagac gatcccgcag
     2341 tggccgtggt cggtgtactc gcagaggcag acgtcggcga tgaccacgac gccgggcgcg
     2401 tgcttcttga tggcggcgag ggcgcgcgcc accgggccct tcgggtcggc ggcgccgctc
     2461 cccacggcgt ccttgacgtc ggggatcccg aagaggagga ccgactggac gccgagcgcc
     2521 accgccctgt ccacctgggc ggtgatgcgg tcgaccgacc actgcgagtg ccccggcatc
     2581 ggcgcgatgg gccgttcgac gttcttcccc tcggtcacga agagggggag gacgaattgc
     2641 gctggtgaga gccgggtctc gcggacgagg ccgcgcatcg cggcgctccg acgcaggcgc
     2701 cgcatctgca cggacgacgg tgagctgctc atctggacct cacggagtac gtgggacggc
     2761 cgtccgcgca ccgagcgcgg cgaccaccgc atcggccagg ccgacggcat cgtgctgcag
     2821 ggccacggcc gcgaccttga gcccgcgtgt ttcgacctgg cgggcggtga ccggcccgat
     2881 cgcgacgatg gcgggcgccg catcgccgcc gaggccgctc gagccggcgc gttccagcgc
     2941 gtcgatcgcg atgtccaccg ccgacgggct cgcaaaggcg atcgcgtcga ccgggcgctc
     3001 cagcagcaca cgcgcgagcc cgccgcccgc ctggtcggga agggtgtggt acgccaccac
     3061 gtcgaccacc tcggcgccgg cgcggcggag cgcctcgggc aggtcgctcc gcgccaggtc
     3121 ggaccgcggc aggaggatcc gccgtcccgc caccgcgccg agcgcggcgg ccaggtcggc
     3181 gccggtggcg cgcgccggtt gcgcggtcgg cgtcaccccc tgctcccgga gcgcggtcgc
     3241 ggtcgcgtcg cccacggcgg cgatagtgac gtcgtccagc gcgcccggtc cgatgccgag
     3301 cgccgccatc cggccgaacg tcgcggcgac ggcgttgacg ctggtgaagg cgatgatgtc
     3361 aaagctggtc agcgagtgga gcgcggcatc gagcggggcg ggatcggcgg cggggacgaa
     3421 ctggatcatc gggacgacga tcaccgtggc gccgcgggat tcgaggagct cggtgagggc
     3481 acgcgactgt tcgcgggagc gggtgagcac caccgtccgg cccgacagcg cgcccgactc
     3541 aggcccgcgt gacatccgcc gccccgcgga ggagctccgc gcccccttcg gccaggagct
     3601 tcgacgccag cgccgcggcg agcgccggac cctccccggt catgcctttc gtcgagccgc
     3661 gcaccacccg gccatccact gcgccgatca tcgcgtggag ggagagcgcc gttccctgga
     3721 cccgggcgtg cgccgccacg gcggagctgc accccccgcc caccgcgccg aggaacgccc
     3781 gctccgccag caccgcggcc tcggtgtcgg ggtcgttgag cgcgcgcacc atcgccacga
     3841 cctcgggccg ggcggcgttc acctcgatgg ccagggctcc ctgccccggt gcgggcagca
     3901 tcgtctcgac cgggaggaag tggatggtgg cgccgtccgc gccgctccgc acgccgagcc
     3961 gctcgagccc cgcggcggcg aggaggaggg cgtcgtagcc gccatcggcg agtttccgga
     4021 gccgggtgcc gacattgccg cggacgtcga ggagttcgat gtcggggcgg agcgccttga
     4081 gctgcgcgct ccggcgcggg ctgctggtgc cgacccgcgc gccgaccggc agcgctgcga
     4141 caccgtcgaa tttcgacacc agcgcgtcac gcggatcggc ccggcgcgcg aaggcggcga
     4201 tccgcatgtc ggccgggagc tccgagggga ggtccttggc gctgtgcacc gccaggtcgg
     4261 cgcggccatc gcggagcgcc gattcgatct ccgccacgaa gagccccttg tcacccaggg
     4321 tcgacagggg ccggtcgagg aggatgtcgc ccttggtggt gatccggagg gtgtcgacct
     4381 cgagccccgg ccacgcacct cggagcgcca aggcggtgag gtcggtctgc accagcgcca
     4441 gcgggctccc acgggtgccg atggtgagct tcttcaaggg tgcgctcatc gggccgccgc
     4501 ggaggcgagg ggcgcgtcgg cacgggcgcc gagttcccgg acccattcga cggtgcgctg
     4561 cacctgctcg accggcgtgg ccggatggag cccgtggccc aggttgaaga tgtgccccgg
     4621 gcgcccgccg gcccgccgca gcacgtcggc gatggcgccc ttcaggtgct ccggcgatcc
     4681 gcagagcgcc atcgggtcga ggttcccctg gatgcccagc gaatggccga cctgcgccca
     4741 cgcccggtcg agcgggatgc gccagtcgac gccgatgacc ccggcgccgt cggtcttcat
     4801 cgcgtcgagg agggtggcgg tgttggtgcc gaagtggatg agcggcacgc cggcggcgcg
     4861 gagctcgccg aagatccggc gggcgtacgg ctggacgaac tcggcgtagt cctgcggcga
     4921 gagcgcgccc acccagctgt cgaagagctg cagcgcgccg gcgcccgcct cggcctgcgc
     4981 ccgcaggtac gggatgatga tggcggtgag ccgctccatc agcgcgtgcc agagcgccgg
     5041 ggcggagtac atcatcgcct tggtgtggcg gaagtcgcgc gaggggcgcc cctcgacgag
     5101 gtacgcggcc agggtgaacg gcgccccggc aaacccgatg accgggatcc gtccgccgag
     5161 ctcccggtcg acgatccgga tggtctcgag gacatgcggc acgtcctggc ccggctcgat
     5221 gggccggagc gcctcgaggt cggcgttcgc ccggatcgga tgaccgacca ccgggcccac
     5281 gtcgtccacc agcgtcacgt cgacgccgat ggccacgagc ggcgagacga tgtcggcaaa
     5341 gagcaccgcc gcgtcgacgt cgaagcgcgc caccggctgg agtgtcacct cggcgcagag
     5401 ctcggggacc tgggtgatct cgaggagggt gcggccctcg cggaccgcgc ggtactcggg
     5461 aaggacgcgc cccgcctggc gcatgaacca gacgggggtg cgttcgaccg gctcccgccg
     5521 cgcggcgcgg cagaagagcg tatcgcggac gttcacatgc ggatcccgga ggccgcgcgc
     5581 tccgcgcggg gcccgccggt gacgccgaag agccgctcca ccgccgccgc gatctcgggc
     5641 tccggcgcct cgttgagcgc cgtgaggggc ccgtgcatca gcttgttgac gatgcgcgcg
     5701 gccatctggc ggagcgccgc ctgctccagt tccgaga
//
LOCUS       DASUAP010000135         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1066748, whole genome shotgun sequence.
ACCESSION   DASUAP010000135 DASUAP010000000
VERSION     DASUAP010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066748"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFU45_03195"
     CDS             complement(<1..495)
                     /locus_tag="VFU45_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5358102.1"
                     /translation="MRPTIPGWRITLAFATVYLVWGSTYLAIRYAIGSIPPFLMAGTR
                     FVVAGGALYLWARLRGVPRPTPAEWRSAAVSGALMLVCGNGAVVWAEQHVASGLTALL
                     AALVPAWTVVAEWLLPGGRAPGRLTTVGLVAGLGGMTLLAGAGPTTGAPVRWRRRCSR
                     SHRSP"
     gene            583..945
                     /locus_tag="VFU45_03200"
     CDS             583..945
                     /locus_tag="VFU45_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU5358103.1"
                     /translation="MPDRATEPVLDPSMLANLRRIGGPAVLTRFIDTFLDYAPGRLTA
                     AEDALRAGDVAAVATVAHGLISSAGQLGAAQLSAASREIEALANAGDLAAVVARSAAW
                     RQAFDAAVTALRATRAGP"
     gene            942..1331
                     /locus_tag="VFU45_03205"
     CDS             942..1331
                     /locus_tag="VFU45_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5358104.1"
                     /translation="MTDLPRVAVVEDNTDNRLLLQALLADRYTVDEYESGPAALAGCA
                     AAPPRVILLDFSLPGMDGAEVLRRLRNDPALARIPVIALTAHAMSGDRERFLEMGFDG
                     YVTKPIIDETILYGAIARLLAGRPEGA"
     gene            1331..>2554
                     /locus_tag="VFU45_03210"
     CDS             1331..>2554
                     /locus_tag="VFU45_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEU5358105.1"
                     /translation="MPDRAIRRHSLAWFGTGIVLLTATGVITYRSLEQFSGAVTLEGH
                     TYEVIEAASQFRTAIRTAESSSRGYALSGAPNLRATYELSRRQAVTALDRLALLTTDN
                     PEQQARLDSLAPIVHAKLGFSDELTGLGATPDATRRLFASGRGQRLMDSIEARIAVVS
                     NAERHLLDVREDQAETSGRAAIGVVVVGGMLGVLLMLAGAQAGWAEIEERRRIEERLA
                     VEAERQAVIIEVQQAVATAPPASDELMRLIADQVMHLFGADGAGIALPDGGDVVYRML
                     VGMLEQFPGLRLKIAGSLVGKVFSTGVPALVQDTHADPDVNRALAEQVGFRSAIAIPL
                     SRGGVTIGVLNVVARRPGAFGDDELRGARIMAGLLSAAFTNAAAFEANQLLLAELRQS
                     RDAAEAANRTKSQFLA"
BASE COUNT          353 a          956 c          922 g          323 t
ORIGIN      
        1 aggtgagcga tgcgaccgcg agcaccgccg ccgccaccgg accggagccc ccgtcgtcgg
       61 tcccgcgccg gccagcagcg tcatcccgcc cagcccggcg acgagaccca ccgtggtgag
      121 ccgccccggc gcccgtcctc cggggaggag ccattccgcc accacggtcc acgccggcac
      181 cagcgcggcg aggagcgcgg tgagccccga cgcgacgtgt tgctcggccc agaccactgc
      241 cccgttcccg cagacgagca tcaacgcccc cgacaccgcc gccgaccgcc actcggccgg
      301 cgtgggtcgg ggcacgccgc gcaaacgggc ccagaggtag agcgcgccgc ccgcgacgac
      361 gaaccgcgtc ccggccatca ggaagggcgg gatggatccg atggcgtagc ggatggcaag
      421 gtaggtcgag ccccagacga ggtagaccgt ggcgaaggcg agcgtgatcc gccagccggg
      481 gatggtgggt cgcatgcgaa ggaggataat caccgctcca acccggatct tgcgcggtcg
      541 ggcgcttgcc gggtgcccgg ggacgtggta gtgtcccgag cgatgcccga ccgcgccacc
      601 gagcccgtcc tcgacccgtc gatgctcgcc aacctccgcc ggatcggcgg cccggcggtg
      661 ctcacccgct tcatcgacac cttcctcgac tacgcacccg ggcggctgac ggcggcggag
      721 gacgcgctgc gcgcgggcga cgtggccgcg gtggccaccg tggcacacgg cctcatttcg
      781 agcgccggcc agctcggggc ggcgcagctg tcggcggcct cgcgcgagat cgaggccctc
      841 gcgaacgccg gcgacctcgc ggcggtcgtc gcccgctccg ccgcctggcg gcaggcgttc
      901 gacgcggcgg tcacggcgct ccgcgccacc cgggccgggc catgaccgac ctcccgcggg
      961 tggcggtggt ggaggacaac accgacaacc ggctgctcct gcaggcgctc ctggccgacc
     1021 ggtacaccgt ggacgagtac gagagcggcc ccgccgcgct cgccgggtgc gccgccgcgc
     1081 cgccgcgggt catcctgctc gacttctccc tcccggggat ggacggcgcc gaggtgctgc
     1141 gccgcctgcg taacgacccg gcgctcgcgc ggatcccggt catcgcgctc acggcccatg
     1201 cgatgagcgg tgaccgtgaa cgcttcctgg agatgggctt cgacggctac gtgaccaagc
     1261 ccatcatcga cgagaccatc ctctacggcg cgatcgcccg gctgctggcc ggccggcctg
     1321 aaggcgcctg atgcccgatc gcgcgatccg acgccacagc ctggcctggt tcggcaccgg
     1381 gatcgtgctg ctcacggcca cgggtgtcat cacctaccgg agcctcgagc agttctccgg
     1441 cgcggtgacc ctcgagggcc acacctacga ggtgatcgag gcggcgtcgc agttccggac
     1501 cgcgatccgg accgcggagt cgagctcgcg ggggtacgcc ctctccggcg ccccgaacct
     1561 ccgcgcgacg tacgaactct cccggcgtca ggcggtcacg gcgctggacc ggctcgccct
     1621 cctcacgacc gacaaccccg aacagcaggc gcggctcgat tcgctggcgc cgatcgtcca
     1681 cgccaagctc ggattcagcg acgagctcac gggcctcggt gccacgcccg acgccacccg
     1741 ccggctcttt gcttccgggc gcggccagcg gctgatggat tccatcgagg cccggatcgc
     1801 ggtggtgagc aacgccgagc gccacctgct cgacgtgcgg gaggaccagg ccgagaccag
     1861 cggccgcgcg gccatcggcg tggtggtcgt cggcgggatg ctcggcgtcc tgctgatgct
     1921 ggcgggagcc caggccggct gggccgagat cgaggaacgg cgccgcatcg aggagcggct
     1981 ggcggtcgag gccgagcggc aggccgtgat catcgaggtg cagcaggccg tggcgaccgc
     2041 gccccccgct tccgacgagc tgatgcggct catcgcggac caggtgatgc acctcttcgg
     2101 ggccgacggc gcggggatcg cactgccgga cggcggggat gtcgtgtacc ggatgctggt
     2161 ggggatgctg gagcagttcc cggggctccg gctcaagatc gccggcagcc tggtgggcaa
     2221 ggtcttttcc accggcgtgc cggcgctggt gcaggacacg cacgccgatc ccgacgtcaa
     2281 ccgggcgctc gcggagcagg tgggcttccg ctccgccatc gcgatcccgc tctcccgcgg
     2341 cggcgtgacc atcggcgtcc tgaacgtggt ggctcgccgc ccgggcgcct tcggcgacga
     2401 cgagctccgc ggcgcccgga tcatggcggg gctcctctcg gccgcgttca ccaatgcggc
     2461 ggcgttcgag gccaaccagc tgctgctggc cgaactgcgc cagagccggg acgccgcgga
     2521 ggccgccaac cggaccaaga gccagttcct ggcc
//
LOCUS       DASUAP010000136         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_657848, whole genome shotgun sequence.
ACCESSION   DASUAP010000136 DASUAP010000000
VERSION     DASUAP010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657848"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..839)
                     /locus_tag="VFU45_03215"
     CDS             complement(120..839)
                     /locus_tag="VFU45_03215"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEU5358106.1"
                     /translation="MPPIRFRSAPDPEAPSLTLPPGELLARRLELLGVLAVPGLRGLT
                     LHANRSVMLSYGANGILRIHEGYAEAPDYVLEAVVRFLLPGIPRPMRKVAETTFLAFP
                     IEHRSEPRRRPERPQPGDLELLHRLGELHARLNAEYFGGALGPIAFRLSGRMRSRLGE
                     LAVDLKTGRPSEIALGRGHVRRHGWAEVEHTVLHEMVHQWQAENGHPVDHGAGFRKKA
                     REVGITPSARRRLGRAERKAG"
     gene            complement(808..1662)
                     /locus_tag="VFU45_03220"
     CDS             complement(808..1662)
                     /locus_tag="VFU45_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5358107.1"
                     /translation="MTDLTIVQLSDLHFGRDADLDQVRALESLIPTLGADAAVFAGDL
                     TQRARHGEFQRALALVEHVQRSTPTLVIPGNHDVQWWRAVLPIGRARVRWEKYRRYFG
                     EALAPVLDVPGAVISGALTSYGLSWGSLTWNLRDLTVKGHLPRRETQRLTKLFADAPP
                     EAVRVAVLHHNVLPGAISRRMGMAHWRSAQRRLLATGADVVLCAHDHQEGAGQVDGVL
                     PVSTSSTHTGRTRGRRPSAFNLVHVDDQAVHIQHWRWESGRREFIPSDINVYARRRRA
                     PDPVPVSA"
     gene            complement(1659..>2321)
                     /locus_tag="VFU45_03225"
     CDS             complement(1659..>2321)
                     /locus_tag="VFU45_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358108.1"
                     /translation="AEEQKVGEFHVYTLPGRLTVSPGVTTTTALFDPVSAPYQKTNVI
                     AGALPWWGGFGQDENAADVPVQVTYVVKRPRKTDLGDRPLPGGIVRMFEPDSAGRLQL
                     IGETNIDHTPAGEDLELNAGTAFDLTARRVQTSWQTGRDSSQSRRFVRTYAVASYADT
                     LRNAGTTDVTVDVYEVRAGEWSVLASSVPAERLSSTKARFRVKVPAGGQALLTYKVRA
                     VW"
BASE COUNT          314 a          823 c          846 g          338 t
ORIGIN      
        1 tctccgatct ggattccttg ttgcgggtaa gaggtgagcg gtaagcggtc agcgggctgc
       61 cggaacagat ctcgaacgcc cgcccgctca ccgcttacag ctcacctctt accgaacgat
      121 caacccgctt ttctctccgc ccgccccagc cgccgcctgg cgctcggcgt gatccccacc
      181 tcgcgcgcct tcttccggaa gccggcgccg tggtccaccg ggtggccgtt ctcggcctgc
      241 cactggtgca ccatctcgtg gaggacggtg tgctccacct cggcccagcc gtgccgccgc
      301 acgtggccgc ggcccagcgc gatctcgctc ggccgccccg tcttgaggtc gaccgccagc
      361 tcgcccaggc ggctccgcat ccggccgctc agccggaacg cgatcggccc cagcgcgccc
      421 ccaaaatact cggcattgag ccgggcgtgc agttcgccca ggcggtgcag gagctcgagg
      481 tcgccgggct gggggcgttc cggccggcgc ctgggctccg accggtgctc gatcgggaag
      541 gccaggaacg tggtctccgc caccttccgc atcggccgcg ggatccccgg caggaggaac
      601 cgcaccaccg cctcgagcac gtagtcgggt gcctcggcgt atccctcgtg gatgcggagg
      661 atcccgttgg cgccgtagct cagcatcacc gagcgattgg cgtggagcgt caggcccctg
      721 agccccggca cggcgagaac gccgagcagc tccaagcgcc gcgccaggag ttcgccgggc
      781 ggcagggtga ggctcggggc ctccgggtca ggcgctgacc ggaaccggat cgggggcacg
      841 gcgcctccgg gcgtacacgt tgatgtccga cgggatgaat tcccgccggc ccgattccca
      901 ccgccagtgc tggatgtgca ccgcctggtc gtcgacgtgc accaggttga acgccgaggg
      961 gcggcggccc cgggtgcggc cggtgtgggt gctcgaggtg ctcaccggca gcacgccatc
     1021 gacctggccc gcgccctcct ggtggtcgtg ggcgcagagg accacgtcag cgccggtggc
     1081 caggagccgg cgctgggcgg agcgccagtg ggccatcccc atccggcggg agatggcgcc
     1141 ggggagcacg ttgtggtgca gcaccgccac ccgcaccgcc tccggcggcg cgtcggcgaa
     1201 gagcttggtc aggcgctgcg tctcccgccg gggcaggtgg cccttgacgg tcaggtcgcg
     1261 gaggttccag gtgagcgagc cccaggagag gccgtagctc gtgagcgcgc ccgagatgac
     1321 cgcaccgggc acgtcgagca ccggggccag tgcctcgccg aagtaccggc ggtacttctc
     1381 ccagcggacc cgcgcccggc cgatcgggag caccgcgcgc caccactgca cgtcgtggtt
     1441 gcccgggatg acgagcgtgg gggtggaccg ctggacgtgc tccaccaggg ccagcgcccg
     1501 ctggaattca ccgtgccggg cgcgctgggt caggtcgccg gcaaagaccg cggcgtcggc
     1561 gcccagggtg gggatgagcg attcgagggc gcggacctgg tcgaggtcgg cgtcgcgccc
     1621 gaagtgcagg tcggagagct ggacgatcgt gagatcggtc accagaccgc ccggaccttg
     1681 tacgtgagga gcgcctggcc tcccgccggc accttgaccc ggaaacgggc cttggtggac
     1741 gagagccgct ccgccgggac cgagctcgcg agcacgctcc actcacccgc gcgcacctcg
     1801 tacacgtcga ccgtcacgtc ggtcgtccct gcgttgcgga gcgtatcggc gtaggaggcg
     1861 acagcgtagg tccggacgaa ccggcggctc tgggaggagt cgcgccccgt ctgccagctc
     1921 gtctgcaccc ggcgggccgt gagatcgaag gccgtgccgg cgttgagctc caggtcctcg
     1981 ccggcaggcg tgtggtcgat gttggtctcg ccgatgagtt ggagccggcc ggcgctgtcg
     2041 ggctcgaaca tccggacgat gccgccgggg agcggtcggt cgcccagatc ggtcttccgc
     2101 gggcgcttga cgacgtaggt cacctgcacc ggcacgtcgg cggcgttctc gtcctgcccg
     2161 aagccccccc accaggggag cgcaccggcg atgacgttcg tcttctggta gggtgcgctc
     2221 acagggtcga agagcgcggt ggtcgtcgtg acgccgggcg agaccgtcag ccgaccgggc
     2281 agggtgtaga cgtggaactc ccccaccttc tgctcctccg c
//
LOCUS       DASUAP010000137        12610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_780458, whole genome shotgun sequence.
ACCESSION   DASUAP010000137 DASUAP010000000
VERSION     DASUAP010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 12610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12610
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780458"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1912
                     /locus_tag="VFU45_03230"
     CDS             68..1912
                     /locus_tag="VFU45_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5358109.1"
                     /translation="MKIGRAAAIAYLRPVGALVLLSAGAIARLMDPVLSDWIWMAGVV
                     LIGVPMVARTLSGMFRGRFAADIVAALAVLSAAVLFHPLAGLVIVLMQSGGEALERHA
                     EGRASRAVEALAAVAPRVAHLVRGATVTDEPVDAVRPGDLILVRPGELIPCDGTVEHG
                     FAPVDMSRITGEPLPIAAGPGSTLLSGGAVLDSPLTIRAVRAAGESEYAQIVQLVRTA
                     QASKAPFQRIADRYAVWFTPITLAVAGLAWAASHDPTRVLAVLVVATPCPMILATPVA
                     MIGGINRGARRGLIFRHGGALEAVAGVTVVVMDKTGTLTLGRPEVAEVAAIPPFSPET
                     ILALTAAVERGSGHELARSVAAEAIARHVAVPGADQIREEPGQGVSGVADGHRVMIGS
                     WSYVAGRSPEAGRAFAPVHGDGTGLRAFVSVDGAAAGMIRFADRARAEAAGVLSRMRA
                     MGIERIMLLSGDRDAEARAVAAGLGITDARGDNLPGDKLAVIHDLIAEGHRVAMVGDG
                     VNDAPALSAATVGVALASGGGRISAEAADIVLLRNDLEGVPAAMAIGRRTMRIARQSL
                     WAGLGLSGIAMIVAAAGQIPPTLGALLQEAIDVAVILNALRSSTEGIR"
     gene            1909..2352
                     /locus_tag="VFU45_03235"
     CDS             1909..2352
                     /locus_tag="VFU45_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5358110.1"
                     /translation="MSVRPRFSDLDPAESEALLARHHIGHLAFAHAGQVDVEPIHYVR
                     QGEWVYCRTAEGSKLTALGERPWVALEVDEIEGPFDWKSVVAHGTVYLVGRGPEHRAA
                     HAETLAAIRAFHAEALTPDDPVPERTTLMRIHVRKLTGRQASTRP"
     gene            2363..2839
                     /locus_tag="VFU45_03240"
     CDS             2363..2839
                     /locus_tag="VFU45_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358111.1"
                     /translation="MNWSAAHIHLLVNHLPILGAPFVLLVLAWGYLTKSRDVTRLALW
                     LMIPLALTGFLADQTGDSAKDQVRNAAWFDKALVHEHEERADSTVIVFYVVAAVGALA
                     LWRRRRHPDERWPLGLAALALAVASLLAAWTGLAGGVIRHDEVRPGAAVSAVPGGE"
     gene            complement(2858..3559)
                     /locus_tag="VFU45_03245"
     CDS             complement(2858..3559)
                     /locus_tag="VFU45_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358112.1"
                     /translation="MFEWLRIRLQQGTRTSAFPAGAVEFPERFRGLPILPPDPDAPPF
                     DLGRCLFGPEDVGGAGVGTPIAYSRDHRMASSTRAGLISPTGQVELARALDAEMLRLF
                     RRSLRLRAVVAGSCGGCEAELTALGNVVFDLARFGIQFVASPRHADGLVITGARNRNM
                     QGALRAAYDAMPGPRIVIAVGACAIAGGPFRGSPEVGAVDDIPVDLYIPGCPPHPLTM
                     LDGLLRLLGRIPGAA"
     gene            complement(3593..5104)
                     /locus_tag="VFU45_03250"
     CDS             complement(3593..5104)
                     /locus_tag="VFU45_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358113.1"
                     /translation="MSAIDDVGLRNRRAIPVAAVPVAPVESFRRAVIEAVRGGGRIVA
                     LFGTPDRGDATRLFAIVADDAQGTLNATSTLVTDRYPALTPDCPQAHAFEREIAEQCA
                     VRPDGHPWLKPLRRHLPDHRSPRRDAVVLDPAVHPFFQVDGSEVHEVAVGPVHAGIIE
                     PGHFRFQAHGEDILFLETMLGYQHRGVERLLETEGPGQAVLVAESIAGDTVIGHVGAY
                     AGAVEAVSRCRKTPRAQAIRGIALELERLANHIGDLGAIAGDVAFQPAAAYFGRMRGE
                     CLNLLMTLSGNRYGRGLVRPGGVTFDLPSAMASEMRSRLAVLRGELEPVAALMFEAAS
                     VQARLEGVGAVTRAQCAEFGFVGPVARACEVARDVRHDHPYGIFRFAHIPVATGWAGD
                     AHARALVRWHEVRRSLAFVVEQLGALPSGAVRTPIDALRPDEITVALQEGWRGEIAHV
                     LITDDRGGIRRHKVVDPSFHNWMAVALALPGNQISDFPLCNKSFNLSYAGHDL"
     gene            complement(5101..6540)
                     /locus_tag="VFU45_03255"
     CDS             complement(5101..6540)
                     /locus_tag="VFU45_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5358114.1"
                     /translation="MRLVLLCLLPAVATAAALLARAPRAGRWRVGLLVATAAAHLALT
                     ASLWVAPAAPAFDGWLAADALGSIVLTLTSILFFVAATYAVGYLRRETPRGGRLFAGG
                     LLGFLTATTVVALSQHLAMMWVGMEATTLAVAPLIFHRHDRRSLEAVWKYLLISSVGI
                     ALALLGIFFLAAAQHDAGGAPLVLGDLLAHAGELQPAWLRAAFLFLVVGFGTKMGLAP
                     MHTWKPDTYGEAPALVGGLMAGALTGCAFLGVARITSVTVAAGLGPFVQPVLLGFGLL
                     SLTVAAAFIIGQRDVKRLLAYSSVEHMGLLVLGLGLGGVGAYGAALHLVNNGLAKGWL
                     FLVAGNVVLATGTSAAADTRGLVRTLPVSGTLLVLGLFAITGSPPFGLFLSEFTILRA
                     AVAGGHGWIALAVLLLLAIIFVGMASLLLGMALGEPAPGTPIVRESAWLVAGPMALAA
                     IVLLLGVYIPAPLHAALARAAAALGGSAP"
     gene            complement(6540..7178)
                     /locus_tag="VFU45_03260"
     CDS             complement(6540..7178)
                     /locus_tag="VFU45_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358115.1"
                     /translation="MQTAAEVLLLLVVLTNFAVLGTSRLRTCIHALAAQGLLLGSLPL
                     ALAAPVTVHTGALAAGTVLVKAVALPWFLGWAIREASVRREVEPVVGFIASLLLGALG
                     LALAFAIAARLPLVEVREPLLVPVALATVVAGLIVITTRRKALTQVVGYLMLENGIYV
                     FGLTQAQSVPFLLEVGVLLDVFAGVFIMGIVVFHINREFDSLDSTRLTELKD"
     gene            complement(7181..8104)
                     /locus_tag="VFU45_03265"
     CDS             complement(7181..8104)
                     /locus_tag="VFU45_03265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEU5358116.1"
                     /translation="MNGPPDVSALAALAVLLLVAPALPGVAVKTKSVLTGRRGAPVLQ
                     LYWDLGKLLRKGAVYSPTTTWVFRAGPVVVLASLLAAALLLPLNGRSALVWFPGDLVA
                     FAALLGLARFALVLAGMDTGSSFEGMGASREVTIASFAEPTLFLCFIALVLATGQLSL
                     GDMLGVALGPAWARAAAPLSLVAIALFVILLAENSRVPIDDPATHLELTMIHEVIVLD
                     HSGPDLALILFGSALKLALFAALVVGVLVPRAGQGPAASLGLFVAGLVAVAVTVGVVE
                     SVMARIRLMRVPQLLVGAGALALFGILLLLR"
     gene            complement(8101..10065)
                     /locus_tag="VFU45_03270"
     CDS             complement(8101..10065)
                     /locus_tag="VFU45_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5358117.1"
                     /translation="MSLIALSVLLIVASGIAAAVLDRRPAVGEAVFRAGLAVGAVLGG
                     AAAVRVLAGGSSPDVVLATGTPGGPWVLGLDALSALFLLTIMLAGTAAAFFGVTYLRR
                     PAAVRGAGTAHFLVALLIAALELVVIARAVVPFLVAWEVMALASYALIVTEHADRAIR
                     RAGMLYLVATHVGTLALFGLFAVWGSGTPDLTFATLSARALGPLPQAAVLVAALVGFG
                     MKAGIVPMHFWLAEAHAAAPSHVSALMSGIVIKMGIYGILRIALLQRAEPEWWGWLVL
                     ALGAASGVLGVVWALVQHDLKRLLAFHSVENIGIIACGIGAGSLGVAYHHPLVAVLGF
                     GGAALHTVNHALFKSLLFLGAGSLAHATGTREIDRLGGLVRHLPGTMTVFLVGSVAIA
                     GLPPLNGFVSEWLVFRSLLAAGLAPGAIRVAVLVVAALGLIGGLAVACFAKVGSAIFL
                     GNPRTPLGRTPHESAPGMLWPMYGLAAACVVIGVVPSLAVGPALRVGAFVAGVPLSAA
                     GIPTSDPAASMVGWLGVAVLALIAVGLGARALLLRRRGRRQAATWACGFPHPTPRMQY
                     TGSSFAAPLAAAFGAVSGVRVDRSPTALATHARDPVLHGFVTPAWAGVQAVLARVRPL
                     QRGRLAVYVLYIVAALVAALIYLVLAGPAA"
     gene            complement(10062..12059)
                     /locus_tag="VFU45_03275"
     CDS             complement(10062..12059)
                     /locus_tag="VFU45_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5358118.1"
                     /translation="MTPLALAILCWSAGAAAAVVARRRAVGTFAGVVGAVAGGGAAAF
                     AALHALVGAAPAWSMPWRVPAGALGLRLDALSAVFLLPIGLIGAVCAVYGVAYARRHG
                     PGAGRNGPLAAYNVLLLSMAVVISAADLVLLIVAWELMALSSWALVVGDHRDPAVRRA
                     GMQYLVAGHVATAALLLFALFTGAATGSFAVAALGHSATLPTGILFVLLLVGFGTKAG
                     IVPLHVWLPDAHPAAPSHVSALMSAVMISTGFYGLCRFLPLLGPPPAWWGALFMVLGA
                     AGMIGGILYSITQRDVKRALAYSTVENAGLVTLAMGAGLLGTARHHPVLAGLAWTAAL
                     LHLWNHAFAKALLFLGFGAVAQRTGSRDLDAMGGILRRWKAVGALVVLGAAAIASLPG
                     LNIFTGEWLLLRGLFSGMLALGGADRIALIGGVVALAFTGALAVAGFTRLVAVGLLGR
                     PRTVGAADAEPPELAMRLPMALLGAACLLMAAVPGRMAGGLAGAVAVIAPAADLSVAQ
                     PALAPLGMLLPGLTALALIVFAVRSATAGRERRREGPTWACGYPGPSARAQYTSTSFA
                     DGLTAIMQPLLRRDPPPAVGVLPATASAPGVAWPVAAAWASTTRDRVLTGVFLPLFAA
                     VARAGERLRALHDPRVTRSLVYLVATVLVLLGLLFLPGVTQ"
     gene            complement(12056..>12610)
                     /locus_tag="VFU45_03280"
     CDS             complement(12056..>12610)
                     /locus_tag="VFU45_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5358119.1"
                     /translation="AVEKGIPVSRRLLEQAWQAPDTVPPLSALVAIGGVHRDVGGRTK
                     AEVLRHVVALLPLPADVDREFLVTVLEAREAMGSTGIGDGIAIPHVRNPILLHVAQSF
                     VALCLLAHPVEFDAVDGKPVHALFVAVSANVPAHLRTLAQLGFALRDPELRRLLAVAA
                     PTAEILARISAVEASLGAGHGSAR"
BASE COUNT         2015 a         4621 c         4613 g         1361 t
ORIGIN      
        1 ccggtgagca gctcatcgcg acggtgcgtg gggtcgggtt cgtgctgcgg cggaacggca
       61 aggacgagtg aagatcgggc gcgccgccgc gatcgcgtac ctccggccgg tcggcgccct
      121 cgtactcctc tccgctggcg ccatcgcccg cctgatggac ccggtcctga gcgactggat
      181 ctggatggcg ggcgtcgtgc tgatcggggt cccgatggtg gcgcggaccc tgtcggggat
      241 gttccggggg cggttcgccg ccgatatcgt ggcagcgctg gcggtgctga gcgcggcggt
      301 cctcttccac ccgctggccg gcctcgtcat cgtcctgatg cagtccgggg gcgaggcgct
      361 ggagcggcac gccgaaggcc gggcctcccg ggcggtcgag gcgctggcgg ccgtggcccc
      421 gcgcgtggcc cacctggtgc gtggcgccac cgtgaccgac gagccggtgg acgccgtccg
      481 ccccggtgac ctcatcctgg tgcggcccgg cgagctcatc ccctgcgatg gcacggtgga
      541 gcacggcttc gcaccggtgg atatgtcgcg gatcaccggc gaaccgctcc cgatcgcggc
      601 gggccccggc agcacgctgc tgagcggcgg cgccgtgctc gattccccgc tcacgatccg
      661 ggccgtccgg gccgccgggg agagcgagta cgcgcagatc gtgcagctgg tgcgcaccgc
      721 gcaggcgagc aaggcgccgt tccagcggat cgcggaccgc tacgcggtct ggttcacccc
      781 gatcacgctg gccgtggcag gcctggcatg ggcggcatcc cacgacccga cgcgggtact
      841 cgcagtgctg gtggtcgcca ccccgtgccc gatgatcctg gcgacgccgg tcgcgatgat
      901 cggcgggatc aaccgggggg cgcgccgcgg gctcatcttc cgccacggcg gcgcgctcga
      961 ggcggtggcc ggggtcacgg tggtggtgat ggacaagacg gggaccctca cgctcggccg
     1021 gccggaggtg gcggaggtgg cggccatccc gcccttctcc ccggagacca tcctggccct
     1081 caccgcggcc gtggagcgcg ggtccgggca cgagctggcg cggagcgtcg ccgccgaggc
     1141 gatcgcacgc cacgtcgccg ttcccggcgc cgaccagatc cgggaggaac ccggccaggg
     1201 cgtgagcggc gtcgccgacg gccaccgggt gatgatcggg tcgtggagct acgtcgccgg
     1261 ccgttcgccc gaagcgggcc gggcgttcgc gccggtgcac ggggacggga ccgggctccg
     1321 tgccttcgtg tcggtggacg gggccgcggc ggggatgatc cggttcgccg accgcgcgcg
     1381 cgcggaagcc gccggcgtgc tgtcgcgcat gcgcgcgatg ggcatcgagc ggatcatgct
     1441 gctgtccggc gaccgcgatg ccgaggcgcg ggcggtcgcg gccgggctcg gcatcaccga
     1501 cgcccgcggc gacaacctgc cgggcgacaa gctcgcggtg atccacgacc tcatcgccga
     1561 ggggcaccgg gtggccatgg tcggcgacgg cgtcaatgac gcgccggcgc tcagcgccgc
     1621 gacggtcggc gtggcgctcg cctcgggcgg cggacgcatc tcggcggagg ccgccgacat
     1681 cgtcctgctc cgcaacgacc tcgagggcgt ccccgcggcc atggccatcg gccgccgcac
     1741 catgcgcatc gcccggcaga gcctgtgggc cggcctgggc ttgagcggga tcgcgatgat
     1801 cgtggccgcg gccggccaga tcccgcccac gctcggcgcc ctcctgcagg aggccatcga
     1861 cgtggcggtc atcctgaacg cactgcgcag cagcacggag ggtatccgat gagcgtccgt
     1921 ccccgattca gcgacctcga cccggccgag tccgaggcgc tcctggcgcg ccaccacatc
     1981 ggccacctcg cattcgccca cgccgggcag gtcgacgtcg agccgatcca ctacgtgcgg
     2041 cagggggagt gggtctactg ccggaccgcc gaggggtcga agctcaccgc gctgggcgag
     2101 cgcccctggg tcgcgctcga ggtcgatgag atcgagggtc cgttcgactg gaagagcgtg
     2161 gtggcccatg ggaccgtcta cctcgtgggg cggggaccgg agcatcgcgc cgcgcacgca
     2221 gagaccctcg ccgccatccg ggccttccac gccgaggcgc tgacgcccga cgacccggtg
     2281 ccggaacgca cgacgctcat gcggatccac gtccgcaagc tcaccggccg ccaggcttcc
     2341 acccgtccct gaggaccgaa ccatgaactg gtcagccgcc cacatccacc tgctggtgaa
     2401 ccaccttccg atcctcggcg cgccgttcgt gctgctcgtg ctcgcctggg gatacctcac
     2461 caaatcgagg gacgtgaccc gcctggcact ctggctcatg atcccgctgg cgctcaccgg
     2521 cttcctggcc gaccagaccg gcgattccgc caaggaccag gtgcgcaatg ccgcgtggtt
     2581 cgacaaggcg ctggtgcatg agcacgagga gcgcgccgat tcgacggtga tcgtgttcta
     2641 cgtggtggcg gccgtcgggg cgctggcgct ctggcgtcgg cgccgccatc ccgacgagcg
     2701 gtggcccctc ggcctcgccg ccctggcgct tgccgtggca tcactgctgg ccgcgtggac
     2761 cgggctcgcc ggcggcgtca tccggcacga cgaggtgcgg ccgggggccg cggtgtccgc
     2821 cgtcccgggc ggcgagtgac cccgggggcg gccggcgcta cgcggcgccg gggatccgcc
     2881 ccaggagtcg gaggagcccg tcgagcatcg tgagcgggtg cggcggacac ccggggatat
     2941 agaggtcgac cgggatgtcg tcgaccgcac ccacctccgg gctgccccgg aacggtccgc
     3001 cagcgatggc gcacgcgccc accgcgatca cgatgcgcgg gcccggcatc gcgtcatagg
     3061 cggcccgcag ggctccctgc atgttccggt tccgggcgcc ggtgatcacg agaccgtccg
     3121 cgtggcgcgg cgacgcgacg aactggatac cgaaccgcgc caggtcgaag accacgttgc
     3181 cgagcgccgt gagctcggct tcgcatccgc cacaactccc cgccacgacc gcccgcagcc
     3241 gcaatgaccg ccggaacagg cggagcatct ccgcatcgag ggcccgcgcg agctcgacct
     3301 ggccggtcgg gctgatgagc ccggcccgtg tcgagctggc catgcgatgg tcccggctgt
     3361 aggcgatggg cgtgcccacg ccggccccac cgacgtcctc cggcccgaac aggcagcggc
     3421 cgaggtcgaa cggcggtgcg tcaggatccg gaggcaggat cggcagcccg cggaagcgtt
     3481 ccgggaactc caccgcgccc gcggggaatg ccgaggtccg ggtaccctgc tggagccgga
     3541 tcctcaacca ttcgaacata ccgatcctcc tccgggcaga tgcgccggac cgttacaggt
     3601 cgtggccggc ataggacagg ttgaagctct tgttgcagag cgggaaatcg gagatctggt
     3661 tccccggcaa ggccaacgcc accgccatcc agttgtggaa tgacgggtcc accaccttgt
     3721 ggcgccggat gccgccccgg tcgtcggtga tgagcacgtg ggcgatctcg ccgcgccacc
     3781 cctcctgcaa cgcgacggtg atctcgtcgg ggcgcagggc gtcgatcggg gtccgaaccg
     3841 cgccgctcgg caatgcaccc agctgctcga ccacgaacgc caacgatcgg cgcacctcgt
     3901 gccaacgcac gagggcccgg gcgtgcgcgt ccccggccca gccggtggcg acagggatgt
     3961 gcgcgaaccg gaagatcccg taggggtggt cgtggcgcac gtcacgtgcg acctcgcagg
     4021 cacgggcgac ggggcccacg aagccaaact cggcgcactg cgctcgggtg accgcgccca
     4081 cgccctccag tcgcgcctgg accgacgcgg cctcgaacat cagtgcggcc acgggctcga
     4141 gttcaccgcg cagcaccgcc agccgcgacc gcatctccga cgccatagcg gagggcaggt
     4201 cgaacgtcac accccccgga cgcacgagcc cgcgtccgta ccggttcccc gagagcgtca
     4261 tgagcaggtt gaggcactcg ccccgcatcc ggccgaagta cgcggcggcc ggttggaacg
     4321 cgacgtcgcc ggcgatggcg cccaggtcgc cgatatggtt ggcgagccgc tcgagctcca
     4381 gcgcgatgcc gcggatggcc tgggcccggg gcgtcttccg gcagcgggag accgcctcga
     4441 cggcgccggc ataggcaccc acgtggccga tcactgtgtc gccggcgatg gattccgcca
     4501 ccagcacggc ctgccccggt ccttccgtct cgagcaggcg ctccacgccc cggtgctggt
     4561 accccaacat ggtctcgagg aagaggatgt cctcgccgtg cgcctggaag cggaaatgcc
     4621 ccggctcgat gatcccggcg tgcaccggcc cgacggccac ctcatgcact tcggagccgt
     4681 ccacctggaa gaacgggtgc accgcaggat cgaggaccac cgcgtcgcgc cgcggcgatc
     4741 ggtgatccgg caggtggcgg cgcaacggct tgagccaggg gtggccgtcc gggcgcacgg
     4801 cgcactgttc ggcgatctcc cgctcgaagg cgtgcgcctg cgggcagtcg ggggtcagcg
     4861 ccggataccg gtcggtgacg agcgtgctcg tggcgttcag cgtcccctgt gcgtcgtcgg
     4921 ccacgatcgc gaagagccgg gtggcgtcgc cccggtcggg ggtgccgaac agcgccacga
     4981 tccgcccgcc gccgcggacc gcttcgatca ccgcacggcg gaacgactcc accggcgcga
     5041 cgggcaccgc ggccacgggg atggcgcggc ggttccggag cccgacgtcg tcgatcgcgc
     5101 tcacggtgca ctccccccga gcgcggcggc ggcgcgggca agcgccgcat ggagcggcgc
     5161 agggatgtac acgccgagga ggagcacgat cgccgcgagc gccatcggtc ccgcgacgag
     5221 ccaggcgctc tcgcgcacga tcggcgtgcc gggcgccggt tcgcccagtg ccatccccag
     5281 caggagcgac gccatgccga cgaagatgat ggccagcagc aggagcaccg cgagcgcgat
     5341 ccacccgtgc ccgccggcga cggccgcgcg gaggatggtg aattcgctga ggaagaggcc
     5401 gaagggcggc gatccggtga tggcgaacag gccgaggacg agcagcgtgc ccgacaccgg
     5461 cagcgtccgc acgaggcccc gggtgtcggc cgccgcagac gtgccggtgg ccagcaccac
     5521 gttgcccgcc accaggaaca accagccctt tgccaggccg ttgttcacca ggtggagcgc
     5581 cgccccgtac gcaccgacgc cgccgaggcc caggccgagc accaggagcc ccatgtgctc
     5641 cacgctggaa tacgccagga ggcgcttgac atcacgctgg ccgatgatga acgcggcggc
     5701 cacggtgagg gacaggagcc cgaagccgag gagcaccggc tggacgaaag gccccaggcc
     5761 ggccgcgacc gtcacggagg tgatgcgcgc cacgccgagg aaggcgcagc ccgtcagcgc
     5821 gccggccatc aggccgccca ccagcgccgg cgcttcgccg taggtgtcgg gcttccaggt
     5881 gtgcatcggc gcgaggccca tcttcgtgcc gaatcccacc acgaggaaca ggaacgcggc
     5941 gcgcagccac gcgggctgga gctcccccgc gtgggccagc aggtcgccga gcaccagcgg
     6001 ggcgccaccg gcgtcatgct gtgcggcggc aaggaagaag atgccgagga gggccagcgc
     6061 gatgccgacg gaggagatca gcaggtactt ccacaccgcc tcgagcgagc gtcgatcgtg
     6121 ccggtggaag atcagcggcg ccaccgcgag cgtggtggcc tccatcccca cccacatcat
     6181 ggccaggtgc tggctgagcg ccacgacggt ggtcgccgtg aggaagccga ggagcccccc
     6241 ggcgaagagg cgcccgccgc gcggcgtttc ccgccgcagg tagcccacgg cgtacgtcgc
     6301 cgcgacgaag aacaggatgc tggtgagcgt gaggacgatc gacccgagcg catccgcggc
     6361 gagccaccca tcgaacgcgg gggccgcggg cgccacccag agcgatgctg tgagcgccag
     6421 gtgcgccgcg gcggtggcca ccaggaggcc cacccgccac cgtcctgcgc gcggcgcgcg
     6481 ggcaaggagc gcagcggccg tcgcgaccgc cggaagcagg cagagcagga cgaggcgcat
     6541 cagtccttga gctccgtgag ccgcgtggag tccagcgaat cgaactcgcg gttgatgtgg
     6601 aagaccacga tccccatgat gaacacgccg gcgaacacgt cgagcagcac gccgacctcg
     6661 aggaggaagg gcacgctctg cgcctgggtg aggccgaaga cgtagatccc gttctcgagc
     6721 atcaggtagc ccaccacctg ggtcagcgcc ttgcgtcggg tggtgatcac gatcagcccg
     6781 gccaccaccg tggcgagcgc gaccggcacg aggagcggct cgcgcacttc gacgagcggc
     6841 agccgggccg cgatcgcgaa tgcgagggcc agacccagcg cgccgagcag caacgacgcg
     6901 atgaagccga cgaccggctc gacctcgcgc cggaccgacg cctcgcggat ggcccacccg
     6961 aggaaccacg ggagcgcgac ggccttcacc agtaccgtgc cggcggccag tgcgcccgta
     7021 tgcacggtga ccggcgcggc cagcgccagc gggagcgatc cgagcagcag cccctgggcc
     7081 gcaagggcat ggatgcacgt gcgcaggcgc gacgtcccga gcacggcgaa gttggtcagc
     7141 acgaccagga gcagcaggac ctccgcggcg gtctgcatcg tcagcggagg aggaggagga
     7201 tgccgaagag cgccagcgcg ccggcgccca ccagcagctg cggcacccgc atgaggcgga
     7261 tccgcgccat gaccgactcc acgaccccca ccgtcaccgc tacggcaacg aggccggcga
     7321 cgaagaggcc gagcgacgcg gccggcccct gccccgcccg cgggaccaga accccgacga
     7381 cgagcgcggc gaagagcgcg agcttgagcg cgctgccgaa gagaatgagc gccaggtccg
     7441 gtccgctgtg atccagcacg atcacctcat ggatcatcgt gagctcgagg tgcgttgccg
     7501 ggtcgtcgat cggcacccgc gaattctcgg ccaggaggat cacgaacagc gcgatcgcca
     7561 cgaggctgag gggcgccgcc gcccgcgccc acgccggccc gagcgcgaca ccgagcatgt
     7621 cgcccagcga cagctgaccc gtggcgagca ccagcgcgat gaaacagagg aacagcgtcg
     7681 gctcggcaaa gctggcgatg gtgacctcgc ggctcgcgcc cattccctcg aaactggagc
     7741 cggtgtccat cccggcgagc accagcgcga accgcgccag cccgaggagg gccgcgaagg
     7801 ccaccaggtc gccggggaac cagacgagcg ccgaccggcc gttgagcggg agcaggagcg
     7861 ccgccgcgag cagggacgcc aggacgacca ccgggccggc gcggaacacc cacgtggtcg
     7921 tggggctgta cacggcaccc ttccgcagca gcttgccgag gtcccagtag agctggagca
     7981 cgggcgcgcc ccggcgcccg gtgagcaccg atttcgtctt caccgcgacg cccggcaagg
     8041 ccggcgccac gaggagcagc acggccagcg ccgccagcgc gctcacgtcg ggtggcccgt
     8101 tcatgccgcc ggtcccgcaa gtacgagata gatgagcgcc gccaccagcg ctgccacgat
     8161 gtacaggacg tacactgcca gccggccgcg ctgcagggga cggacccggg caagcaccgc
     8221 ctgcacgccg gcccacgcgg gggtcacgaa cccgtgcagc acgggatccc tcgcgtgggt
     8281 ggcgagggcc gtgggggaac gatccacgcg cacgccggat accgcgccga acgccgcggc
     8341 gagcggtgcg gcgaacgacg acccggtgta ttgcatgcgc ggcgtggggt gcgggaaccc
     8401 gcaggcccag gtggccgcct ggcgccggcc ccggcgccgg agcaggagcg cgcgggcgcc
     8461 gagcccgacc gcgatcagtg ccagcacggc gacaccgagc cagccgacca tcgacgccgc
     8521 cgggtccgag gtcgggatcc ccgcggccga gagcggcacc cctgccacga aggccccgac
     8581 ccggagcgcc ggccccacgg caagcgacgg caccacgccg atcacgacgc aggcggcggc
     8641 cagcccgtac atcggccaca gcatgccggg tgccgattca tgcggcgtcc ggcccagcgg
     8701 tgtccggggg ttgcccagga agatggcgct tccgaccttc gcgaagcagg ccacggccaa
     8761 cccgccgatg agccccaatg ccgccaccac gagcaccgcg acgcggatcg cgccgggcgc
     8821 gagcccggcg gccagcagcg accggaacac cagccactcg ctcacgaagc cgttgagcgg
     8881 cgggaggccg gcgatggcca ccgagcccac caggaagacg gtcatggtgc cgggaaggtg
     8941 gcggaccagc ccgccgagcc ggtcgatctc acgcgtgccg gtcgcgtgcg ccagcgagcc
     9001 cgcgccgagg aagagcaggc tcttgaagag ggcgtggttc accgtatgca gcgccgcacc
     9061 accgaagccg aggacggcca ccagggggtg gtgataggcg acgcccagcg aacccgcgcc
     9121 gatcccgcac gcgatgatgc cgatgttctc cacgctgtgg aacgccagca gccgcttcag
     9181 gtcatgctgc accagcgccc acaccacccc cagcacgccc gacgcggcgc cgagcgcgag
     9241 gaccagccac ccccaccact ccggctccgc acgctgcagc agggcgatgc ggaggatccc
     9301 atagatcccc atcttgatca cgatcccgga catcagcgcc gacacgtgcg acggcgcggc
     9361 cgcatgggcc tcggccagcc agaaatgcat ggggacgatg cccgccttca tcccgaatcc
     9421 gacaagcgcg gccaccagca ccgccgcctg cggcagcggc ccgagcgccc gggcgctgag
     9481 cgtggcgaac gtcaggtcgg gcgtcccgct gccccacaca gcgaacagcc cgaacagcgc
     9541 cagcgtgccg acgtgggtcg ccacgaggta gagcatgccg gcccggcgga tggcgcggtc
     9601 agcgtgttcg gtcacgatca gcgcgtacga cgcgagcgcc atcacttccc aggcgacgag
     9661 gaacggcacc acggcccggg cgatgaccac gagctccagc gccgcgatga gcagcgccac
     9721 caggaagtgc gccgtgccgg caccccggac ggccgccggc cgccgaaggt aggtcacccc
     9781 gaagaaggcg gccgccgtgc cggccagcat gatcgtgagc agaaagagcg ccgagagcgc
     9841 atcgagtccg agcacccacg gcccccccgg tgtgccggtc gcgagcacga catcggggct
     9901 ggacccgccg gccaggacgc gcaccgccgc ggcgccgccg aggacggcgc caacggccag
     9961 ccccgcacgg aacaccgcct cgccgacggc cgggcggcgg tcgaggaccg cggcggcgat
    10021 accggacgcc acgatgagga gcacgctcag ggcgatgagg ctcactgcgt gacgcccggg
    10081 aggaagagca ggccgaggag cacgagcacc gtggccacca gatagacgag cgatcgggtg
    10141 acgcgcgggt cgtggagcgc gcgcagccgc tcgcctgcgc gcgccaccgc ggcgaagagc
    10201 gggaggaaca ccccggtcag cacacgatcg cgggtcgtgc tcgcccaggc ggcggccacc
    10261 ggccacgcca cgcccggggc cgatgcggtg gccggcagca ccccaacggc gggcggcggg
    10321 tcacgccgca gcaacggctg catgatggcg gtgagcccgt cggcgaacga cgtcgacgtg
    10381 tactgcgcgc gggccgacgg ccccggatat ccgcaggccc aggtcggacc ctcgcggcgc
    10441 cgctcgcggc cggccgtcgc ggaccgcact gcgaacacga tgagcgcgag cgcggtgagc
    10501 cccggaagca gcatgccgag cggcgccagg gccggctggg cgacggacag gtccgcggct
    10561 ggtgcgatga cggccacggc gccggccagg cctccggcca tgcgcccggg gaccgcagcc
    10621 atcaggagac aggcggcgcc gagcagcgcc atcggcaggc gcatcgccag ctccggcggc
    10681 tccgcgtcgg cggcgccgac ggtccgcggg cgcccgagga ggccgacggc gaccaggcgg
    10741 gtgaaccccg ccaccgccag cgcgccggtg aacgccagcg ccaccacgcc gccgatcagc
    10801 gcgatgcgat cggcgccgcc caacgcgagc atgcccgaga acagcccccg gagcaggagc
    10861 cactcgccgg tgaagatgtt gaggccgggc agcgaggcga tcgccgcagc gccgagcacc
    10921 accagcgccc ccacggcctt ccagcgccgg aggatcccgc ccatggcgtc gaggtcccga
    10981 ctgccggtcc gctgggccac ggcgccgaaa ccgaggaaca agagggcctt ggcgaacgcg
    11041 tgattccaga ggtgcaggag cgccgcggtc cacgcgaggc cggcgagcac cggatgatgg
    11101 cgggcggtcc ccagcaaccc cgcgcccatc gccagcgtga ccaggccggc attttccacg
    11161 gtcgaatagg ccagcgcgcg cttcacatcg cgctgggtga tcgagtagag gataccgccg
    11221 atcatccccg cggcaccgag gaccatgaac agggcgcccc accaggcggg cggcgggccc
    11281 agcagtggga ggaagcggca caggccgtag aatccggtcg agatcatcac cgccgacatg
    11341 agcgccgaga cgtgcgaggg cgcggcgggg tgcgcgtcgg ggagccagac gtgcagcggc
    11401 acgatgccgg ccttggtgcc gaagcccacg agcaggagca cgaacaggat gcccgtcggg
    11461 agcgtcgccg agtggccgag cgcggcgacg gcgaaactgc cggtggccgc gccggtgaac
    11521 agggcgaaca ggagcaacgc cgcggtggcg acgtgcccgg ccacgaggta ctgcatcccg
    11581 gcccgccgta cggcgggatc ccggtggtcg cccaccacca gcgcccacga gctcaaggcc
    11641 atcagttccc acgcgacgat gagcaggacg aggtcggcgg cggagatgac caccgccatg
    11701 ctcagcagga ggacgttata ggccgccagc gggccattcc tgcccgcacc gggcccatga
    11761 cgccgcgcgt aggccacgcc gtagacggcg cacaccgcgc cgatcaggcc gatcggcagc
    11821 aggaacaccg ccgagagcgc gtccagccgg agcccgagcg caccggccgg cacccgccac
    11881 ggcatcgacc aggccggcgc agcgcccacg agcgcgtgca gcgcggcgaa cgccgcggcg
    11941 ccgcccccgg ccacggcgcc caccacgccc gcgaaagtcc cgaccgcgcg ccgccgtgcc
    12001 accacggccg cggcggcgcc ggcgctccag cagaggatcg cgagggcgag cggcgtcacc
    12061 gggcggatcc gtggcccgcc ccgaggctcg cttcgaccgc actgatgcgc gccaagatct
    12121 cggccgtggg cgcggccacg gcgagcagcc ggcgcagctc cgggtcgcgc agcgcgaagc
    12181 cgagctgggc gagcgtccgc aggtgcgctg ggacgttcgc gctcaccgcc acgaacagcg
    12241 cgtgcaccgg cttgccgtcc acggcgtcga actccaccgg atgcgcgagc aggcagagcg
    12301 ccacgaagga ctgggccacg tgcagcagga tgggattgcg gacgtgcggg atcgcgatgc
    12361 cgtcgccgat gcccgtggaa cccatcgcct cgcgcgcctc gagcaccgtg accaggaatt
    12421 cccgatccac gtcggcgggg agcggcagca gcgccaccac gtgccggagc acttccgcct
    12481 tcgtccggcc cccgacgtcg cggtgcacgc cgccaatggc caccagcgcc gagagcggcg
    12541 gaacggtgtc gggcgcctgc cacgcctgtt cgaggaggcg cctggagacg gggatgccct
    12601 tctccaccgc
//
LOCUS       DASUAP010000138         6585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_617066, whole genome shotgun sequence.
ACCESSION   DASUAP010000138 DASUAP010000000
VERSION     DASUAP010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617066"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VFU45_03285"
     CDS             complement(<1..788)
                     /locus_tag="VFU45_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358120.1"
                     /translation="MAGFAELHLTGALGAALESLGYRADAPEVRDIVPTAARGHNLVF
                     VAPPAPIHAVPALAGAVAHAAAGGEGLRLLVLAPESGIAGFRRALLPLAGAAGLRIHA
                     ATTPARAARRLQDGSADILITTPDNALALVERSALKATALRTLFLAWPEQFADLELLA
                     PLMGEVAKDTQRIIHSADLVRVTDLGERYARRAHTVGLGAAEAPVAPLAGPVRVVTVA
                     EPGRGEALRGILEVLDPETVNVWAADEGTRAEAMAALAAADAGAT"
     gene            941..2149
                     /locus_tag="VFU45_03290"
     CDS             941..2149
                     /locus_tag="VFU45_03290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5358121.1"
                     /translation="MPDPLSPNLEFLKASETVAISAEAKRRKAAGEDVIDLGVGEPDF
                     DTPAPAAEAGIEAIRKGYTRYPPNIGTLDLRSAIAHQLSLLSGGRAVNADHIVVSSGS
                     KQSIFNACFSLFGPGDRVLIPAPAWVSYPQIVHLARAEPVLVPGDPEWGLKVSVKDLD
                     AHAQRARGIILCSPCNPTGSVYTLSELKAIAEWAVTKRVWIISDEIYRRINYGGGPAP
                     SFLDLPDELLERVVVVTGASKAYAMTGWRIGAALAPAHLAKAMAALQSHTTTGANHPA
                     MLAATAAFTDPRVEQDVQRMVAAFRKRRDHVVARFRAEAPGVEFVEPLGAFYFFFRVD
                     GVVSGDVTGGLGFCERLMQDEGVALVPGAAFGDDRWVRLSYAVGDRDLDAALDRVLRF
                     IHKLAAQRAA"
     gene            2159..2662
                     /locus_tag="VFU45_03295"
     CDS             2159..2662
                     /locus_tag="VFU45_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358122.1"
                     /translation="MNLDARSEPLAGPLPSLVWRWDAETDILSGAMAGVARKSGLTGT
                     VELTDDEGSIAVVDVAGGVLRGLDIVVWPEVTEVEGLAAPLPARDAQVVLPPRSSQPG
                     IASLEVDTTLSVAANPNHDVLHLRIGTKRQVEVVRVADRLLVEVDPKKRLAGFWLLGV
                     PQLDETP"
     gene            2836..3606
                     /locus_tag="VFU45_03300"
     CDS             2836..3606
                     /locus_tag="VFU45_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358123.1"
                     /translation="MGEDQPWPGTVAFLDGIQQWEPVASAGVSPIVVADVAAAVRERT
                     GRTFRTAVVRQERVIVARPDALAAAGAAIDGWDTLALPVDEPPHPIGDLFLARAAVER
                     RRSQLEREVGRAYRAGSTGWLVVDGSLTESPEWAADPRMVGVIKSHASLPFAGEALET
                     YLRLPHRTRSSVFRPASRARAPVHSWALRLWPWEGKDLLYGLVRVEVAPSDHPTDRAD
                     ELSRWLLAERAPVSTPDARWDRLLYGIHDVERFLRSER"
     gene            complement(3680..3982)
                     /locus_tag="VFU45_03305"
     CDS             complement(3680..3982)
                     /locus_tag="VFU45_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU5358124.1"
                     /translation="MNAMQERPYCVYILASRSRVLYIGVTNDIPRRIAQHRAGIGSTF
                     TSKYHVDRLVHFEECVDIRSAIAREKQVKGWSRMKKVDLIEAHDAGWIDLAEGWSP"
     gene            4092..6020
                     /locus_tag="VFU45_03310"
     CDS             4092..6020
                     /locus_tag="VFU45_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358125.1"
                     /translation="MPDIIGRVVATELKPSTPHQFYFWTARQSAVGIGAIVRVEGDGR
                     VVWGVVTDAFAYSDLVTPLHDVIGADGDPARTGLAPSERPEIRLWQAAVLRQQPEEPL
                     QPVPLGQVHLATDADVVAALRMDAFTTGDAPTGVPVGVYSAAGLEAPVFLDADFLLGP
                     EAAHLNITGVSGLATKTSAVAFLLSAIFQKFPAHKGSVAAVCFNVKGPDLCFLDQPGE
                     LTDADRRLYARLDLAPRPFDDVTVYAPYKADGVNPNTLRTHEALAVRTEPLVWGLREV
                     LDYAEVLLNKDDIDAKADAFIDFLADRVVGKEFEDRQLRDRPFLVNTFADLEEFFRAI
                     FDYVEALGRAEVWRTHHIATIRKVRNRLGNISTRAKGLVTDDGAASDLPWGRFRDRSA
                     LVVDVAGVDPLAQDLVFARVVSKLREHLERRDLGVDHVVVFVDELNKYAPADGPDTYV
                     RKMLLDLSERGRYLGLVLFSAQQFRSQVQRRVVGNAGTAVYGRMDSDELSMPAYSTLS
                     PAIKSKLASLPKGELMLRHPHFTQPIFVRFPRPAVLSGRDGVERFPPEPDLPFADAVA
                     RQLKALDRAVARDAVAELVHGRREEDVRRAVHATRRARPDNVLAYFRAQLGARVERES
                     PAPRRGVAPVKGGGDPYS"
     gene            6080..>6585
                     /locus_tag="VFU45_03315"
     CDS             6080..>6585
                     /locus_tag="VFU45_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5358126.1"
                     /translation="MRIAHLADLHLGFRQYHRQNANGINQREADVANAFRAAIDGVIA
                     AAPDCVIVAGDLFHSVRPTNAAIVFAFRQFQRLREALPRTPVILVAGNHDTPRSSETG
                     SILKLFEEIGVDVAAEGPRRLVYPEGNLSVLAVPHQSLMEAPRPSLRPEGNELFQVLV
                     LHGEVEGI"
BASE COUNT          899 a         2404 c         2326 g          956 t
ORIGIN      
        1 gtggcgcccg catcggcggc ggccagcgcc gccatggcct ccgcacgggt gccctcgtcg
       61 gcggcccaca cgttgacggt ctcagggtcg aggacctcga ggatcccgcg cagtgcctcc
      121 ccccggcccg gctcggcaac ggtcacgacc cgtaccggcc ccgcgagcgg tgcgaccggc
      181 gcctcggccg cgccgaggcc caccgtgtgg gcccggcggg cgtagcgctc gcccaggtcg
      241 gtgacgcgca cgaggtcggc gctgtggatg atgcgctggg tgtccttggc cacctcaccc
      301 atcagcggcg cgaggagctc gaggtcggcg aactgctccg gccacgcgag gaagagcgtg
      361 cgcaaggcgg tcgccttgag cgcggagcgc tccaccaggg cgagtgcgtt gtcgggggtg
      421 gtgatgagga tgtcggccga gccgtcctgc aggcggcgag cggcgcgggc cggggtggtg
      481 gcggcgtgga tgcggaggcc ggcggcaccg gcaagcggca ggagggcccg gcggaacccg
      541 gcaatgccgg attcgggcgc cagcaccagc agccggagcc cctcgccgcc cgcggcggcg
      601 tgggccaccg ctccggcgag cgccgggacg gcgtggatcg gggcgggcgg ggccacgaag
      661 acgaggttat gcccccgcgc ggcggtgggc acgatgtcgc ggacctcggg cgcatcggcc
      721 cggtatccca atgactccag tgcggcgccc agggcgccgg tcaggtgcag ctccgcaaat
      781 ccagccacgc caccctcggt tgtcaaagac gttcccgcga tcctgcgggg cggggaacct
      841 acaccgacgc ccggcaccgg ccaagcccgt ggttgacagc gcccgtcacg ttgcggagtt
      901 ttcgccgtcc ggcgccacca cacccgagcc caccgagccg atgcccgatc ccctgtcgcc
      961 caacctggag ttcctgaagg ccagcgaaac ggtcgccatc agcgccgagg cgaagcgtcg
     1021 caaggccgcg ggcgaggacg tgatcgacct gggcgtgggc gagcccgact tcgacacgcc
     1081 ggcgccggcc gcggaggccg gcatcgaggc catccggaag ggctataccc gctatccacc
     1141 caacatcggc acgctcgacc tgcggagcgc catcgcgcat cagctgtcgc tcctctccgg
     1201 cggccgcgca gtcaacgccg accacatcgt cgtgagctcg ggttccaagc agtcgatctt
     1261 caacgcctgc ttctcgctct tcgggccggg cgaccgcgtc ctcatcccgg cgccggcgtg
     1321 ggtgtcgtac ccgcagatcg tccacctggc gcgggccgag ccggtcctgg tgccgggcga
     1381 ccccgagtgg ggcctcaagg tgagtgtgaa ggacttggac gcgcacgccc agcgcgcccg
     1441 ggggatcatc ctctgctcgc cgtgcaaccc gacgggatcg gtgtacacgc tgtcggagct
     1501 caaggccatc gccgagtggg cggtgacgaa gcgcgtgtgg atcatcagcg acgagatcta
     1561 ccgccggatc aactacggcg gcggtccggc gccgtcgttc ctcgacctgc ccgacgagct
     1621 gctggagcgg gtcgtcgtcg tcaccggcgc aagcaaggcc tatgcgatga ccggctggcg
     1681 gatcggcgcg gcgctggccc cggcccacct ggccaaggcg atggcggcgc tccagtccca
     1741 caccacgacc ggcgccaacc accccgcgat gctcgccgcc acggcggcgt tcaccgaccc
     1801 ccgggtggaa caggatgtcc agcggatggt ggccgcgttc cgcaagcgcc gcgaccacgt
     1861 ggtggcgcgc ttccgggccg aggcccccgg cgtcgagttc gtcgagccgt tgggcgcctt
     1921 ctacttcttc ttccgcgtcg acggggtcgt gagcggcgac gtgacgggcg gcctgggctt
     1981 ctgcgagcgc ctgatgcagg acgagggggt ggcgctggtg ccgggcgcgg ccttcggcga
     2041 cgaccgctgg gtgcgcctga gctatgccgt cggcgaccgc gaccttgacg ccgccctcga
     2101 ccgggtcctc cggttcatcc ataagctcgc tgcccagcgg gcggcgtagg ggccacgaat
     2161 gaacctcgat gcacgctcgg agccgctcgc cggacccctg ccgtccctgg tgtggcggtg
     2221 ggatgccgag accgacatcc tctcgggcgc catggccggc gtggcccgga agtccgggct
     2281 caccggcacg gtggaactca ccgacgacga aggctcgatc gcggtggtgg acgtggcggg
     2341 cggcgtgctc cggggcctgg acatcgtggt gtggcccgag gtcaccgagg tcgagggcct
     2401 cgccgcgccg ctgccggccc gcgacgcgca ggtcgtgctg ccgccccggt cgtcccagcc
     2461 cggcatcgcg tcgctcgagg tcgacaccac gctctccgtg gcggccaacc cgaaccacga
     2521 cgtgctccac ctccgcatcg gcaccaagcg ccaggtcgag gtggtgcggg tcgccgaccg
     2581 cctgctggtc gaggtggacc cgaagaagcg gctcgcgggg ttctggcttc tcggagtgcc
     2641 gcagctcgac gagaccccct gaccatcgcc ggcgccgtcc cccgcgtcac cgccgtcgcg
     2701 gcgctccgcc gcgcgctcgg cggggccggc ctttcgcccg cccgcagcat ccccgaagcc
     2761 agcgaagggc gcgccgagca ggcggcgatc cacccggccg acctcgtcga gccggggccc
     2821 cttgccgctc atccagtggg cgaagaccag ccgtggccgg ggacggtcgc cttcctcgac
     2881 ggcatccagc agtgggaacc ggtggcctcg gcgggcgtct cgccgatcgt cgtcgccgac
     2941 gtcgccgcgg cggtacggga gcgcacggga cgcaccttcc gcactgccgt ggtccggcag
     3001 gaacgggtga tcgtcgcgcg cccggatgcg ctggccgcgg ccggtgccgc catcgatgga
     3061 tgggacaccc tcgcgttgcc cgtcgatgag cccccccatc cgatcggcga cctctttctg
     3121 gcccgcgccg cggtggaacg gcgacggagc cagctggagc gcgaggtcgg acgcgcctac
     3181 cgcgccggct cgaccgggtg gctggtggtg gatggctcgc tcaccgagtc gccggagtgg
     3241 gccgcggatc cacggatggt gggcgtcatc aagagccacg cgtcgctccc tttcgccggc
     3301 gaggcgctcg agacctacct ccgcctgccg caccggacgc ggtcgtcggt gttccggccc
     3361 gcgtcgcgcg cccgggcgcc ggtgcacagc tgggcgctcc ggctctggcc ctgggagggc
     3421 aaggacctgc tgtacggcct ggtgcgggtg gaggtggccc cgtccgacca tccgacggac
     3481 cgggccgacg aactctcccg ctggctcctc gcggaacggg cgccggtgag cacgcccgat
     3541 gcgcggtggg accggttgct ctacgggatc cacgacgtgg agcggttcct tcggagtgaa
     3601 cggtgaacgg tgaacgggga acggagaacc gggtgctcgc atgtgtcatc ccgagcggag
     3661 cgagggacct ggccggccgt cacggtgacc agccttccgc caggtcgatc cacccggcgt
     3721 catgtgcttc gatcaggtcc accttcttca ttcgggacca tcctttgacc tgcttttccc
     3781 gcgcgatcgc cgatcggatg tcgacgcact cctcgaagtg gacgagtcgg tcgacgtgat
     3841 atttggacgt gaaggtggat ccgatccctg ccctatgctg ggcgatccga cgcgggatgt
     3901 cgttcgtcac accgatgtag aggactcggc tgcgacttgc caggatgtag acacagtagg
     3961 ggcgctcctg catggcattc atggtgggcg cactacgtca acgcggtcac aaaccgggca
     4021 ggtccctcgc tccgctcggg atgacgatcc gaccccaccg gcccgggcct tcggagagta
     4081 gattgccccc catgcccgac atcatcggcc gcgtcgtcgc caccgagctc aagccgagca
     4141 cgccgcacca gttctacttc tggacggcgc ggcagtccgc ggtcggcatc ggcgccatcg
     4201 tccgggtcga gggcgatggc cgggtggtct ggggggtggt gactgacgca ttcgcctact
     4261 ccgacctggt cacgccgctg cacgacgtga tcggcgccga tggcgacccg gcccggaccg
     4321 gactcgcccc gtcggaacgc ccggagatcc ggctctggca ggccgcggtg ctccgccagc
     4381 agccggagga gccgctgcag ccggtgccgc tcggccaggt ccacctggcc accgacgccg
     4441 acgtcgtggc ggcgctccgc atggacgcct ttaccaccgg cgacgctccg accggcgtgc
     4501 cggtcggcgt ctactcggcc gcggggctcg aggccccggt gttcctcgac gccgacttcc
     4561 tcctcggccc cgaggcggcg cacctcaaca tcaccggcgt ctccgggctc gccaccaaga
     4621 cgagcgcggt cgccttcctc ctctcggcca tcttccagaa gttcccggcg cacaagggct
     4681 cggtcgcggc ggtgtgcttc aacgtcaagg gcccggacct ctgcttcctc gaccagccgg
     4741 gcgagctcac cgacgccgat cgacggctgt acgcgcgcct cgacctcgcg ccgcggccct
     4801 tcgacgacgt gacggtgtac gccccctaca aggccgacgg cgtcaacccc aacaccctgc
     4861 ggacccacga ggcgctcgcc gtgcggacgg agcccctcgt gtgggggctc cgcgaggtgc
     4921 tcgactacgc cgaagtgctc ctcaacaagg acgacatcga cgccaaggcc gatgccttca
     4981 tcgacttcct ggccgatcgc gtggtcggga aggagttcga ggaccggcag ctccgcgacc
     5041 ggccgttcct ggtcaacacc ttcgccgatc tcgaggagtt cttccgggcc atcttcgact
     5101 acgtcgaggc gctcgggcgg gccgaagtgt ggcgcacgca ccacatcgcc acgatccgca
     5161 aggtccgcaa ccgcctgggc aacatcagca cccgggccaa ggggctggtc accgatgacg
     5221 gcgccgccag cgacctgcca tggggccggt tccgcgaccg gagcgcgctg gtggtggacg
     5281 tggccggggt cgatccgctg gcgcaggacc tcgtcttcgc ccgggtggtc tccaagctcc
     5341 gcgagcacct cgagcgccgc gacctggggg tcgaccacgt ggtggtgttc gtggacgagc
     5401 tcaacaagta tgcccccgcc gatggccccg acacctacgt ccggaagatg ctgctcgacc
     5461 tctcggagcg ggggcgctac ctgggcctgg tgctcttcag tgcccagcag ttccgcagcc
     5521 aggtccagcg gcgcgtggtc ggcaatgccg gcaccgcggt gtacggccgg atggacagcg
     5581 acgagctctc gatgccggcc tactcgacgc tctcgcccgc catcaagagc aagctcgcct
     5641 cgctgcccaa gggggagctg atgctccgcc acccgcactt cacccagccg atcttcgtgc
     5701 gctttccgcg gccggcggtg ctgtcggggc gggacggcgt ggagcgcttc ccgccggagc
     5761 ccgacctgcc gttcgccgac gcggtggccc ggcagctcaa ggcgctcgac cgcgccgtcg
     5821 cccgggatgc cgtggcggag ctggtccacg gccggcggga ggaggatgtg cgccgcgccg
     5881 tccacgccac ccgccgggcg cggcccgaca atgtgctcgc gtatttcagg gcccagctcg
     5941 gcgccagggt ggagcgggag tctccggcgc cgcgtcgcgg cgtggccccg gtgaagggcg
     6001 ggggggaccc ctactcgtag cctgcgggat gggcccccgc ccgctgtccg acccgccatc
     6061 cttccgctat ccttaatggg tgcgcatcgc ccatctggcc gatctccacc tgggcttccg
     6121 gcagtaccac cgccagaacg ccaacggcat caaccagcgt gaagccgacg tagccaatgc
     6181 gttccgcgcc gccatcgacg gcgtgatcgc ggcggcgccc gactgcgtca tcgtcgccgg
     6241 cgacctgttc cattccgtcc gacccaccaa cgccgccatc gtcttcgcct tccgccagtt
     6301 ccagcggctc cgcgaagcgc tcccccgcac gccggtcatc ctggtggcgg ggaaccacga
     6361 taccccgcgc tcgtccgaga cggggtcgat cctcaagctg ttcgaggaga tcggcgtgga
     6421 cgtcgcggcc gaggggccgc ggcgcctggt gtatcccgag ggcaacctca gcgtgctcgc
     6481 cgtgccgcac cagtcgctga tggaggcgcc ccggccatcc ctccgtcccg aggggaacga
     6541 gctcttccag gtgctggtac tgcatggcga ggtcgaggga atttt
//
LOCUS       DASUAP010000139         5098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_794245, whole genome shotgun sequence.
ACCESSION   DASUAP010000139 DASUAP010000000
VERSION     DASUAP010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794245"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFU45_03320"
     CDS             <1..874
                     /locus_tag="VFU45_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358127.1"
                     /translation="STSATPAGAAKQTLSGLTDAQVRGAYVFSDALVATLMLNLPTGT
                     KFDSAQAQAAGASASNWLLFPVNSFANGFSATAGLAGTRHAGAWNLGVALSGRLNSSF
                     KPVTGDSLSYKPGFEGRVRLGADRLVGQARLTLGLTFSTYGDDAFGAGAGAQQRYSPG
                     KRFIGEASFTTPGLGGMVTAYAWDYFRAAGDTAGISTGNRENILTAGASHRIGIGQNT
                     SLESVAEARVLSQQGGGSGVLVGIGTGLRVRMSDRFNLVPNARVDFGTAKFPTGGSAS
                     VTGVSASALIQYTF"
     gene            1049..1408
                     /locus_tag="VFU45_03325"
     CDS             1049..1408
                     /locus_tag="VFU45_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358128.1"
                     /translation="MASELGNAQISTLLETLPGIATVLRSPVADALLNVIRAAARLTD
                     FAVDDAEELVRYATRRGLLGIEEGDRLLLDIHAADQKRHDRAALRAKDAKSKARAHPK
                     PKRPKPKHPKKPAKKRR"
     gene            complement(1470..3164)
                     /locus_tag="VFU45_03330"
     CDS             complement(1470..3164)
                     /locus_tag="VFU45_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5358129.1"
                     /translation="MKSMRSLRWIVTAGMGVIIVLSVVSGVFSIVALGRVRKAIDASL
                     AALVQTNALGDGLVASALDEVRAAEEYMVQPSPQVQRDFQAAGDSAIATMTSYRRLSS
                     LSTDDRYTLNEMATAQAEFDVSYSLAHALVDLGRAADGRKMADQARGAADTLVADIHR
                     LTASQTQVALAGSAVLRTQAMWWEKVVGGILILVVVFGSLIGRYTVTQVSGPLGRLVG
                     AVERFGQGDLRPFQVGSMPLELARLATAMDEMGSRLRQVVGAVVRESQTISASASDFS
                     AMSEELAASSGEISTAMVKVSSSAEIQVQGLRDADQLLNTLRAAATSNADASARVVRL
                     GEEIRTVAARHRKDVGAAGATLLDVREVVQTSAKEVQELAKLSESITEFIDLIKQISS
                     QTNLLALNAAIEAARAGEHGRGFAVVADEVRNLADSSARAAEDVAKTVKVIREQVRDV
                     ASTMEVGTKKVAGIEDVASAAATGLEAIATAIQEIEAAAAQVAAEAERNRTVVDAVSH
                     TTAQVAMAANEHASSSEEVTAAAEEQSASTEEMASSASELLQGANRLTDLVSSFKT"
     gene            complement(3161..4267)
                     /locus_tag="VFU45_03335"
     CDS             complement(3161..4267)
                     /locus_tag="VFU45_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5358130.1"
                     /translation="MPFLLLAALACGGGGQKPIKIGLQSTFGDPLGLPLRYGAQLAAQ
                     EINAQGGIHGRKIEFVEADDYGDADSAVAAATRLAQSDVVAVVGGAFSGPTLSAAAVY
                     NDPAHQVVEISPSASSPEVTFAGDWTFRVCAGDLAHAARLASFVRQQLNLTRGAVLYM
                     DNQYGRGFREVFTSEFKKLGGQVLLSGPYLPDKPGDTGPYFDLIKKSGQVQFLLAASY
                     ETDGAELLRQLKKRGLNIPLLGGDGLEGLQREGAIADGSYQTAAYLPAVATPENQAFV
                     KRYRQAFPTINPPNQTAAATYDIVKLLAQVIGDVGTDRKAIRNALAEVGTQRPTFVGV
                     TGPIQFDANGDVPTKKVLIAMVKGGAVQLVEGGQ"
     gene            complement(4451..4945)
                     /locus_tag="VFU45_03340"
     CDS             complement(4451..4945)
                     /locus_tag="VFU45_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358131.1"
                     /translation="MLLLAAAGRGLSAQAAPVAFEFLGFRPGMARPAIDTAAHAAGAD
                     SLECQASQADPHLSECRASLSTADADRTVDLWFSAVDDTATVLTLSARLTSARLDRWR
                     EFLSARYGAPPPRTRGPMTMLQWISDRRMLRLTWRAHGRGFDASVSMVDGRVLDAWGN
                     RAFP"
BASE COUNT          732 a         1807 c         1803 g          756 t
ORIGIN      
        1 cagcacctcc gccacgccgg ccggggccgc caagcagacg ctgagcggcc tgaccgacgc
       61 gcaggtacgg ggcgcctacg tgttcagcga cgccctcgtg gccacgctga tgctcaatct
      121 tcccaccggg accaagttcg attcggcgca ggcacaggcg gccggcgcga gcgcctcgaa
      181 ctggctgctc ttcccggtca actccttcgc caacggattc tccgctaccg ccggcctggc
      241 cgggacccgg cacgccggcg cctggaacct gggcgtggcc ctgagcggcc gcttgaactc
      301 gagcttcaag cccgtcaccg gcgacagtct gagctataag cccgggttcg aggggcgcgt
      361 ccggctgggg gccgatcgcc tggtcggcca ggcgcgcctc accctcgggc tcacgttcag
      421 cacgtacggc gatgacgcct tcggcgccgg tgccggcgcc cagcagcggt acagtcccgg
      481 caagcgcttc atcggcgagg cctccttcac gactccgggg ctcggcggga tggtcacggc
      541 gtatgcgtgg gactacttcc gcgcggccgg cgacactgcg ggcatctcca ccggcaatcg
      601 cgagaacatc ctcaccgccg gcgcctccca tcggatcggg atcggccaga acacctccct
      661 tgagtcggtc gccgaggccc gggtcctctc ccagcagggc gggggaagcg gcgtcctcgt
      721 cgggatcggc accggtctcc gagtgcggat gtccgaccgg ttcaacctgg tcccgaacgc
      781 ccgcgtggac tttgggaccg ccaagttccc gaccggcgga tcggcaagcg tcaccggggt
      841 cagcgcctcg gcgctcatcc agtacacgtt ctagcccagc gccgaccccc taccgagcct
      901 gccaaaacgg cggcctgcca ttggcgggtc gccgtttcgt tcagcgcttg acggggtcgt
      961 gggtttgcgg tacgttggcg ccgatttcgg cggtcggagc agccgctccc gccccggact
     1021 ccggcagtcc ccgcaaagga gatgggcagt ggcctcggaa ctcggcaacg cacagatctc
     1081 gaccctcctg gagaccctcc cggggatcgc caccgtgctc cgcagcccgg tggccgatgc
     1141 actgctcaac gtgatccggg cggcggcgcg gctcaccgac ttcgcggtgg atgacgccga
     1201 agagctggtg cggtacgcca cccgtcgggg gctcctcggg atcgaggaag gcgaccgcct
     1261 gctgctggac atccacgcgg cggaccagaa gcgccacgac cgggccgcgc tccgcgccaa
     1321 ggacgccaag tccaaggcgc gtgcccaccc gaagcccaag cgccccaagc cgaagcatcc
     1381 gaagaagccg gccaagaagc gccggtagcc tgaagcacga gggggtcgcc tgcaggcgac
     1441 cccctcggtc gttcaggcga tcgggccggt caggtcttga acgaggagac caggtcggtc
     1501 agccggttgg ccccctggag gagttcgctg gccgaggacg ccatctcctc ggtcgaggcg
     1561 ctctgttcct ccgccgcggc ggtgacctcc tcgctcgacg acgcgtgctc gttggccgcc
     1621 atcgccacct gggcggtggt gtggctcacg gcgtcgacga ccgtgcggtt ccgctccgcc
     1681 tcggcggcca cctgcgcggc ggcggcctcg atctcctgga tcgcggtcgc gatcgcctcg
     1741 aggccggtgg cggcggccga ggcgacgtcc tcgatgccgg ccaccttctt ggtgcccact
     1801 tccatggtgc tcgccacgtc ccgcacctgt tcccggatga ccttgaccgt cttggccacg
     1861 tcctcagcgg cgcgggcgct cgaatcggcc aggttccgca cttcgtcggc gacgaccgcg
     1921 aagccgcggc catgctcgcc ggcgcgcgcg gcctcgatgg cggcgttgag cgcgagcaag
     1981 ttggtctgcg acgagatctg cttgatcagg tcgatgaatt cggtgatcga ctcggagagc
     2041 ttggccagct cctgcacttc cttggccgag gtctgcacca cctcgcgcac gtcgagcagc
     2101 gtggcgccgg cggcgccgac gtccttccgg tggcgtgccg cgacggtgcg gatctcctcg
     2161 cccaggcgca ccacccgggc cgaggcgtcg gcgttgctcg tggcggcggc gcggagggtg
     2221 ttgagcagct ggtccgcgtc gcggagcccc tgcacctgga tctccgcgct gctggagacc
     2281 ttgaccatcg cggtggagat ctcgccgctc gaggcggcca gctcctcgct catcgcggag
     2341 aagtcgctgg cgctggcgga gatggtctgg gactcccgca cgaccgcgcc cacgacctgc
     2401 cggagccggg agcccatctc gtccatggcg gtggccaggc gcgccagctc gagcggcatg
     2461 ctcccgacct ggaacggccg gaggtcgcct tggccgaagc gttccacggc gccgacgagg
     2521 cggccgaggg ggccgctcac ctgggtcacg gtgtaccggc cgatgaggct gccgaagacg
     2581 accaccagga tcaggatccc gccgaccacc ttttcccacc acatcgcctg ggtgcgcagc
     2641 accgccgacc cggccagcgc cacttgggtc tggctcgcgg tgagccggtg gatgtcagcg
     2701 acgagggtgt cggcggcgcc ccgggcctgg tcggccatct tccggccgtc ggccgcgcgg
     2761 ccgaggtcga cgagggcgtg ggccagggag taggagacgt cgaactccgc ctgtgcggtc
     2821 gccatctcgt tcagggtgta gcgatcatcg gtgctcagtg aggacaggcg ccggtacgag
     2881 gtcatcgtgg cgatggccga gtcgccggcg gcctggaagt cgcgctggac ctgcggcgaa
     2941 ggctggacca tgtactcctc ggccgcccgg acctcgtcga gggcggacgc gacgagcccg
     3001 tcacccaggg cgttggtctg gaccagcgcc gcgaggctgg cgtcgatcgc cttgcggacc
     3061 cgcccgaggg cgacgatgct gaacacgccg ctcacgacgc tcagcacgat gatgacgccc
     3121 atccccgcgg tgacgatcca cctgagactc cgcatcgact tcattgtccc ccctccacca
     3181 gctggaccgc accgcccttg accatcgcga tcagcacctt cttggtcggg acgtccccgt
     3241 tggcatcgaa ctggatcggc ccggtcacgc ccacgaatgt ggggcgctgc gtccccacct
     3301 cggccagcgc gttccggatg gccttccgat cggtgccgac gtcgccgatg acctgcgcca
     3361 ggagcttgac gatgtcatag gtcgccgccg cggtctggtt gggcgggttg atcgtcggga
     3421 acgcctgacg atagcgcttc acgaacgcct ggttctcggg ggtcgcgacc gccggaaggt
     3481 acgcggcggt ctggtaggag ccgtccgcga tggccccttc ccgctggagc ccctcgagcc
     3541 cgtcgccgcc caggagcgga atattgagcc cgcgcttctt gagttgccgc aggagttcgg
     3601 cgccgtcggt ctcatagctg gcggcgagca ggaactggac ctggccgctc ttcttgatga
     3661 ggtcgaagta cgggccggtg tcccccggct tgtccggcag gtaggggccg ctcaggagca
     3721 cctggccgcc gagcttcttg aactccgagg tgaagacctc gcggaagccg cggccgtact
     3781 ggttgtccat gtagagcacg gcgccgcgcg tgaggttgag ctgctgccga acgaaggacg
     3841 cgaggcgggc ggcgtgggcc aggtcgccgg cacacacccg gaaggtccag tcgcccgcga
     3901 acgtcacctc cggcgagctg gccgacggcg agatctcgac cacctggtgc gccgggtcgt
     3961 tgtagaccgc cgcggcggag agcgtcggtc cggagaaggc gccccccacc accgccacca
     4021 cgtccgattg ggccaggcgc gtggccgcgg ccaccgcgga gtcggcatcg ccgtagtcgt
     4081 cggcctcgac gaactcgatc ttccgtccgt ggatgccgcc ctgcgcgttg atctcctgcg
     4141 ctgcgagctg ggcgccgtac cggagcggga gcccgagcgg gtcgccgaaa gtgctctgca
     4201 ggccgatctt gatgggcttc tgcccgccgc cgccgcacgc gagcgcggcc agcaacagga
     4261 acggcaccga gcgacgccag atgcgagcgc cggccggtga ggcgggtgcg tgaccggaca
     4321 aagccacgag gagctccctt tggacgcgga gacgggatcc cgccgaaggt ggaagcgggt
     4381 atccgatgtt caacttatgg cgcggtcagg ggggtggtaa gaccggctca aatgtaactc
     4441 cacaacggag ttacggaaag gcgcggttcc cccaggcatc cagcacccgg ccgtccacca
     4501 tgctcaccga ggcgtcgaat ccccggccgt gggcccgcca ggtgagccgc agcatccgcc
     4561 ggtccgagat ccactgcagc atcgtcatcg gcccgcgggt gcgcggcggc ggggcaccat
     4621 atcgggccga caggaactcc cgccagcggt cgagcctggc cgaggtcagc cgggccgaca
     4681 acgtgagcac cgtggccgtg tcatcgacgg ccgagaacca gaggtcgacg gtgcggtcgg
     4741 cgtcggcggt cgagaggctg gctcggcact cgctcaggtg cggatcggcc tggctggcct
     4801 ggcattccag gctgtcggcg ccggccgcgt gcgccgcggt gtcgatggcg gggcgcgcca
     4861 tcccgggacg gaagccgagg aactcgaagg ccaccggcgc cgcctgcgcg ctgagccccc
     4921 ggccggcggc ggccaggaga agcaccaaaa gccaccgccc gcctcgcgcg agtggggtgc
     4981 cgagcggggc gggcgggacg tcgtgcctca cgaagctggg tcgcgttcag cctgcccggc
     5041 ggacgttcga ggcctgcagc cccttcgggc cctcgacgac gtcgaattcc accggctc
//
LOCUS       DASUAP010000140         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_739562, whole genome shotgun sequence.
ACCESSION   DASUAP010000140 DASUAP010000000
VERSION     DASUAP010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739562"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFU45_03345"
     CDS             <1..968
                     /locus_tag="VFU45_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5358132.1"
                     /translation="HVLRGDVTVTFARPLPPAAAHDLAALPGVLAVEPYRAVPVRIGA
                     LQRSRLIVLLGLPPDAELRRIVGSDERAVPAPAGGLLLTAKLADVLRVRPGDSVRVQV
                     LEGSRRERMMPVVAVVDELLGLSAYADPATAARLAGDGDAISGAVLRTDPAGTPELNR
                     RLKRIPAVAGANSRTDALASFEDTLKRSFTVVTLVLVGFAGGIAVAVVYNAARISLSE
                     RGRELASLRVLGFSRSEVSRMLLGEQVVITSVGIAAGLGVGYLFAAELSALYQWELFR
                     LPLLVTGATYAFAAAVTAGAAAGSAAIVARRIGRLDLVAVLKTRE"
     gene            971..2122
                     /locus_tag="VFU45_03350"
     CDS             971..2122
                     /locus_tag="VFU45_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5358133.1"
                     /translation="MHIPRRYWWWALAVGGGAALVALLGRTRPEPVETAVAGIGAVVT
                     TVDEEGRTRIRDRYLVTAPVVGVVARIGLLEGTPVRAGDVVARLTPSILDPRARREAE
                     ARRDAAADAARSAAASARSARSAWEQAHRERVRAERLAAPGVIPPQERERAEVAESTA
                     TRALEAAEYRARAAQHDVELARAALAGDGALLLGAPVSGKVLRVPERSERPVLAGTVL
                     AELGDPRSLEVVLDLLSADAAQVRPSDPVVVTGWGGLRPLPATIRTIEPSAFTKLSAL
                     GVEEQRVHVIADLAEVPPELGDGFRVDASVVIWRADSVLRIPANALVRRASGWSVYVV
                     EAGRARERAVTVGHQGGGFAEIAGGLKPGEAVIRQPGDRIGDGTRVAAR"
     gene            complement(2125..>2597)
                     /locus_tag="VFU45_03355"
     CDS             complement(2125..>2597)
                     /locus_tag="VFU45_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358134.1"
                     /translation="GWRVRARASAPTQWRLPVEVGIGIEAGRTGAPYSDHTYAVEVSP
                     IVGRHWGPVSFAMNARLERGLGGSGDQEWEFEPAASVRLQVSHPLAVVTEYFSALGAL
                     DAIGPASTQTHLLFPGISLRLGDDVEWNAAVGIGLSPASDALTFKTGIEFPLHD"
BASE COUNT          301 a          964 c          971 g          361 t
ORIGIN      
        1 gccacgtcct gcgcggcgac gtcaccgtca cattcgcgcg accccttccg cctgccgccg
       61 cgcacgacct tgcggccctg cccggcgtgc tcgcggtcga gccctaccgg gcggtgccgg
      121 tgcggatcgg ggcgctgcag cgttcccggc tgatcgtgct ccttggcctt ccgcccgacg
      181 ccgaactccg ccgcatcgtc ggttccgatg agcgggcggt cccggccccc gccggcgggc
      241 tcctcctcac ggccaagctc gccgacgtgc tccgcgtgcg ccccggggac tccgtgcggg
      301 tgcaggtgct cgaagggagc cggcgggagc gcatgatgcc cgtggtcgca gtggtggacg
      361 aactcctcgg cctttcggcc tacgccgacc ccgccacggc ggcgcgcctg gcgggcgacg
      421 gcgacgcgat ctccggcgcc gtcctccgca ccgacccggc aggcacgccg gagctcaacc
      481 gccggctcaa gcggatcccg gcggtggcgg gcgccaacag ccggacggat gcactggcga
      541 gcttcgagga caccctcaag cggagcttca ccgtggtcac cctggtgctg gtcgggttcg
      601 ccggcggcat cgcggtggcg gtggtgtaca acgccgcccg catctcgctc tccgaacgcg
      661 gacgtgaact cgcaagcctg cgggtcctcg gcttcagccg ttccgaggtg tcgcggatgc
      721 tgctggggga gcaggtcgtg atcacgagcg tggggatcgc cgccggcctc ggggtcggct
      781 atctcttcgc cgccgagctc tcggcgctct accagtggga gctcttccgg ctcccgctcc
      841 tcgtcaccgg cgccacctac gcctttgcgg cggccgtcac cgcgggcgct gccgccggct
      901 ccgccgcgat cgtggcccgg cggatcggcc gcctcgatct cgtggccgtc ctcaagacca
      961 gggagtgacg atgcatatcc ccaggcgcta ctggtggtgg gcgctcgcgg tcggcggcgg
     1021 ggcggcgctc gtggcgctcc tcggccgcac tcgtcccgag ccggtcgaga ctgcggtggc
     1081 cgggatcggc gccgtcgtca ccacggtgga tgaggaaggg cgcacccgga tccgcgaccg
     1141 gtacctcgtc accgccccgg tggtaggcgt ggtcgcgcgg atcgggctgc tcgaaggcac
     1201 gccggtccgt gcgggcgatg tggtggcgcg cctcaccccc tcgatcctcg acccccgtgc
     1261 gcggcgcgag gccgaggccc gccgcgacgc cgcggccgac gccgcccgct cggcggcggc
     1321 gtcggcgcgc tcggcgcgct ccgcgtggga gcaggcccac cgcgagcggg tgcgtgccga
     1381 gcgcctcgcg gcgccggggg tgatcccgcc gcaggaacgg gaacgcgccg aagtggcgga
     1441 atcgaccgcc acccgcgccc tcgaggcggc ggaataccgg gcccgcgccg cccagcacga
     1501 cgtcgagctt gcccgcgcgg cgctggccgg cgacggtgcg ctgctgctcg gggcgccggt
     1561 gtcggggaag gtgctccggg tccccgagcg gagtgagcgg ccggtccttg cgggcacggt
     1621 gctggcggag ctcggcgacc cccggtcgct cgaggtggtg ctcgatctcc tctccgccga
     1681 tgcggcccag gtccgcccca gcgacccggt ggtcgtcacc ggctggggcg gtctccgtcc
     1741 gctcccggcc accatccgca ccattgaacc gtcggccttc accaagctgt cggccctcgg
     1801 ggtcgaggag cagcgggtgc acgtcatcgc cgacctcgcc gaggtcccgc ccgagctggg
     1861 ggacggcttc cgggtcgacg ccagcgtggt catctggcgt gccgacagcg tgctgcggat
     1921 cccggccaac gcgctggtgc gccgggcatc ggggtggtcg gtgtacgtgg tggaggcggg
     1981 ccgggcccgc gagcgagcgg tgacggtggg gcaccagggc gggggcttcg ccgagatcgc
     2041 gggcggactc aagcccggcg aagccgtgat ccgccagccg ggcgaccgga tcggggacgg
     2101 gacgcgggtg gccgcgcgct gacgtcagtc gtgcagggga aactcgatgc cggtcttgaa
     2161 cgtgagcgca tcggaggcgg gcgaaaggcc gatgccgacg gcagcattcc attccacgtc
     2221 atctcccagc cggagcgaga tgccggggaa gagcaggtgc gtctgcgtgg acgccggccc
     2281 gatcgcgtcg agcgcgccca gggcgctgaa gtactccgtc accaccgcca gcggatggct
     2341 gacctggagc cgcacgcttg ccgccggctc gaactcccac tcttggtcgc ccgagccgcc
     2401 cagtccccgc tccagccgcg cgttcatggc gaatgacacc ggtccccagt gccggcccac
     2461 gatcggcgag acttccacgg cgtacgtgtg gtcgctgtac ggggcgccgg tgcggccggc
     2521 ctcgatgccg ataccgacct ccaccgggag gcgccactgc gtcggcgcgc tggctcgtgc
     2581 ccggaccctc caaccag
//
LOCUS       DASUAP010000141         6503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_930734, whole genome shotgun sequence.
ACCESSION   DASUAP010000141 DASUAP010000000
VERSION     DASUAP010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930734"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFU45_03360"
     CDS             complement(<1..552)
                     /locus_tag="VFU45_03360"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5358135.1"
                     /translation="MKWPVVLAALAAVAGCGRRPATTAAASGDSLLITVPEAQRGRIT
                     VATVTPQPFAPVVEANGVVAFDGDQSTPVLAPISGPVTRILVEPGAVVRAGQPLAYVA
                     SPDFAAAMAGYRKAAATARQLQRVADLNAKLYQNDGISRRDLEQSQTDAVSAAADRDA
                     ALEQLRALGVDDSTLAALQADRPV"
     gene            complement(554..1819)
                     /locus_tag="VFU45_03365"
     CDS             complement(554..1819)
                     /locus_tag="VFU45_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5358136.1"
                     /translation="MTKAVLCSIAMLAAGAPGLRAQASAPLPLTRGEAVAQALAHNPQ
                     LEVAREQTAQARARLTEAAAFPDLVATADWTGLPRPLQVGGGDGSDYGVGVTLPFPQK
                     FLLRDRVANADYRNFQFAYVQLRQLTAANTIQAYDALLVAQRHGEDLRQADSLAQDFL
                     AKTRARFDAGAVARIDVVKAQVAVAQARNQLIANDRDIANARAALNRLIGRVLGAPIR
                     TADTLAVPGGLPDLDSLVAVAVVTRPEVQGMAAQMRGASAAASLAQQYFLPDVSLGVS
                     KNLVAGSPDTYTFALGFSVPLLFWNHQRGEVAEARHHQRELDASARDLAAQVEQEVRA
                     AWAAADAAIKQAVYVRDQLLPEAREAYRIVSVNYGLGGASSLDVLSAKADLLDAETQY
                     TDALGAANDAVAQLEVAVGTTFTGDPDVR"
     gene            complement(1922..2257)
                     /locus_tag="VFU45_03370"
     CDS             complement(1922..2257)
                     /locus_tag="VFU45_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358137.1"
                     /translation="MRRTVRLRAFLLAALFVGVGSVAPMADAILFHQGGQVARPHIET
                     RDNPACHGERCVIPLGQLTSAGTPAVTPVPTSLAPVADLVVIRSADAPRSSPFPDSRR
                     SRAPPPSFA"
     gene            complement(2371..6498)
                     /gene="rpoC"
                     /locus_tag="VFU45_03375"
     CDS             complement(2371..6498)
                     /gene="rpoC"
                     /locus_tag="VFU45_03375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5358138.1"
                     /translation="MWFFKTLPSPMGNLLNVTLRDLERVIYYSSYIVIEPGEQDVEAN
                     QLLDEEEYLALRQKAKQDGDHAFRADIGAPAVRELLSRIDVKKLAEELRATVADETSQ
                     HRKKQMLKRLKIVDAFLSSGDQGDLPNNPAWMILDVVPVIPPDLRPLVPLDGGRFATS
                     DLNDLYRRVINRNNRLQKLIQHRAPEVILRNEKRMLQEAVDALFDNGRRSKAIRGRGK
                     RPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKTMAVELFKP
                     FIIHKLVEKGIAETVKRAKKIVERESPEVYEILEEIIQDHPVLLNRAPTLHRLGIQAF
                     EPVLVEGKAIRIHPLVCAAFNADFDGDQMAVHVPLSFEAQLEARLLMISSNNILKPSD
                     GRPVAEPSQDMVLGSYWLTKLPPGFEEQVRKAGKNQEAVKALWKDAKWYGTLAELEMA
                     LAHGAITHHDAIHFWYTPPREAADAAPRWIATTAGRVLFNSILPRQLVAELGFRDEVM
                     KKKALSELVLQGYRRAGLAETVLFLDRLKEFGFRFATLGGVSIGIEDLHIPAEKAKLL
                     GEADQRVQRFQRAYNTGQITFGERYNKVIDAWTHANNDVAEAMVNGMRDSRGGFNPVF
                     MMFDSGSRGSRDQIRQLAGMRGLMAKPQKKLTGGIGEIIESPIKSNFREGLTVLEYFI
                     STHGARKGLADTALKTADAGYLTRRLVDVAQDVTITEEDCGTTLGLEMTALKEGEDII
                     EGLRERVMGSVAAEEVYDPHELDEFGDPKLLVQAGQLIDEDASVAIEDAGIEKVKIRS
                     VLTCEARRGVCRMCYGRNLATMDMVDMGEAVGILAAQSIGEPGTQLTLRTFHIGGTSS
                     RIAEDAERRARADGTVHYTKGLEWTDVPVEYEDGLKGSIRIVLTREDESASDETKEGL
                     LVTDPKDHKRVLNRYPVPEGALLQVKEHDAAQGRQTILYTWDPYNDPSILKIDGDLRW
                     KDLVPGVTLREELDESTGLRSLVVQTDPDRELHPSVLVYAPNRKDPQEYTLAEGSRII
                     LGSPTDLVSRAMTVPDEANPWSSKFRVEEVPINVAWPGKTKKEARDKTVWLSPPVKVL
                     TGVTVTKIPRQAYKTRDITGGLPRVAELFEARRPKDPATIAEVDGIVKFGEIKRGKRE
                     IFVYPEEGEAQLYEVAAGKHLRVHEGDRVRAGDRLTEGPVNPHDILRIKGPRAVQEYL
                     LNEVQEVYRLQGVRISDKHIGVIVRQMLQKVRVTDPGDTDFLEGETVDKLLFREENAR
                     VEKKKGDPADSEPVLLGITKASLTTQSFISAASFQETTRVLTDAAIRGAKDDLLGLKE
                     NIIIGHLIPAGTGLYRYAEIDIEPPAGFEPPPPPEPGAEQSAEPVAATVAIGDED"
BASE COUNT          854 a         2232 c         2371 g         1046 t
ORIGIN      
        1 caccgggcgg tcggcctgga gcgcggccaa cgtcgaatcg tcgacaccga gcgcgcggag
       61 ctgttccagg gcggcgtcgc ggtcggcggc cgccgacacg gcgtcggtct gggactgctc
      121 gaggtcgcgc cgggagatgc cgtcgttctg gtagagcttc gcgttcaggt cggccacgcg
      181 ctgcagctgg cgcgcggtgg cggcggcctt ccggtacccc gccatcgccg ccgcgaagtc
      241 gggggaggcc acgtaggcga gcggctggcc cgcccggacc acggcaccgg gctccaccag
      301 gatccgggtc acgggcccgg agatcggcgc cagcaccggc gtcgactggt cgccgtcgaa
      361 cgcgacgacg ccattggcct cgaccaccgg cgcgaacggc tggggggtca cggtcgccac
      421 ggtgatccgc ccccgctggg cctcggggac cgtgatgagc agcgaatcgc cggacgcggc
      481 ggcggtggtg gcgggtcggc ggccgcaccc ggccacggcg gcaagcgccg cgagcacaac
      541 gggccacttc aagtcaacgg acatcgggat ccccggtgaa cgtggtgccg acggccacct
      601 cgagctgcgc cacggcatcg ttggcggcgc ccagggcgtc ggtatactgg gtctcggcgt
      661 cgaggaggtc ggccttggcg ctcagcacat cgagcgacga ggcgcctccc agcccgtagt
      721 tgacggacac gatgcggtac gcctcccggg cctccgggag caactggtcc cgcacgtaga
      781 cggcctgctt gatggcggcg tcggccgcgg cccacgcggc gcgcacctcc tgctccacct
      841 gggccgccag gtcgcgtgcg gacgcgtcga gctcacgctg atggtgccgt gcctcggcga
      901 cctcgccccg ctggtggttc cagaagagga gcggcaccga gaacccgagt gcgaaggtgt
      961 acgtgtcggg gctccccgcc acgaggttct tgctcacgcc gagcgacacg tcgggcagga
     1021 agtactgctg ggcgagcgac gccgcggcac tcgcgccccg catctgggcg gccatcccct
     1081 gcacctcggg ccgcgtcacc accgcgaccg ccaccagtga atcgaggtcc gggagcccgc
     1141 ccgggacggc cagcgtgtcg gcggtgcgga tcggcgcgcc gagcacccgg ccgatcagcc
     1201 ggttgagcgc cgcgcgcgcg ttggcgatgt cgcggtcgtt ggcgatgagc tggttccggg
     1261 cctgcgccac ggcgacctgc gccttgacca cgtcgatgcg cgccaccgcg ccggcgtcga
     1321 agcgcgcgcg ggtcttggcg aggaagtcct gcgccaggga gtcggcctgg cggaggtcct
     1381 cgccgtggcg ctgggccacg aggagcgcgt cgtacgcctg gatcgtgttg gcggcggtca
     1441 gctgccggag ctgcacgtag gcgaactgga agttccggta atcggcgttc gccacccggt
     1501 cgcggagcag gaatttctgg gggaacggga gcgtcacgcc gacgccatag tcactgccgt
     1561 cgccgccgcc gacctggagc gggcgaggca gcccggtcca gtcggccgtc gcgaccaggt
     1621 ccggaaaggc ggcggcctcg gtgaggcggg cacgcgcctg tgcggtctgt tcccgcgcca
     1681 cttcgagctg gggattatgc gccaacgcct gcgccaccgc ctcgccgcgg gtgagcggca
     1741 acggggcgga cgcctgggct cggaggccgg gtgcgccggc cgcgagcatc gcgatgctgc
     1801 agaggactgc cttcgtcatg gactccccct ccgtgcggag ggcgttcgcg ctggaccacg
     1861 ccggatgcgg ccggccggga ctggtgtccc gggccgcgat cacggccgag cgatgttcag
     1921 gtcaggcgaa cgacggaggc ggggcgcggg accggcggga gtcggggaac ggactggagc
     1981 ggggagcgtc ggccgaccgg atgaccacga ggtcggccac cggcgcgagc gaggtcggga
     2041 ccggcgtgac ggcgggcgtg ccggccgacg tgagctggcc gagggggatg acgcagcgct
     2101 cgccgtggca ggcggggttg tcccgcgtct cgatgtgcgg gcgggccacc tggccgccct
     2161 ggtggaacag gatcgcgtcg gccatgggcg ccaccgaccc gacgcccacg aacagggcgg
     2221 cgaggaggaa ggcgcggagg cggacggtgc ggcgcataga cggataaata aggggtacgg
     2281 cccccccggc gcgcaagatt caggcgcggt ccccggggcg gaccgggccc cggaacgacc
     2341 agcgcccggc cggggccggg cgctggaggt tcagtcttcg tcgccgatcg cgaccgtcgc
     2401 ggccaccggc tcggccgact gctccgcgcc cggttccggc ggcgggggcg gttcgaaccc
     2461 cgccggcggc tcgatgtcga tctcggcgta ccggtagagg ccggtgcccg ccgggatcag
     2521 gtgaccgatg atgatgttct ccttgaggcc gaggaggtcg tccttggccc cgcggatggc
     2581 cgcgtcggtg aggacgcggg tggtctcctg gaacgacgcc gcggagatga acgactgggt
     2641 cgtgagcgac gccttggtga tcccgagcag gaccggctcc gaatcggccg gatcgccctt
     2701 cttcttctcg acccgcgcgt tctcctcgcg gaagagcagc ttgtcgaccg tctcgccctc
     2761 gaggaagtcg gtgtcgcccg ggtcggtgac gcggaccttc tgcagcatct ggcgcacgat
     2821 gacgccgatg tgcttgtcgc tgatccgcac gccctgcagg cggtacacct cctgcacttc
     2881 gttgagcagg tattcctgca ccgcgcgcgg acccttgatc cgcaggatgt cgtgcgggtt
     2941 gaccggcccc tcggtcaggc ggtcgccggc gcggacgcgg tcgccctcgt gcacgcggag
     3001 gtgcttgccg gcggcgacct cgtacagctg ggcctcgccc tcctccgggt agacgaagat
     3061 ctcgcgcttg ccgcgcttga tctcgccgaa cttgacgatg ccgtcgacct cggcgatcgt
     3121 ggccgggtcc ttcgggcgcc gcgcctcgaa gagctccgcc acccgcggca ggccgccggt
     3181 gatgtcgcgc gtcttgtagg cctggcgcgg gatcttggtg accgtgacgc ccgtcagcac
     3241 cttgaccggc ggcgagagcc acaccgtctt gtcacgcgcc tccttcttgg tcttccccgg
     3301 ccacgcgacg ttgatcggca cctcctcgac ccggaacttc gacgaccacg ggttggcctc
     3361 gtcggggacg gtcatcgcgc ggctcaccag gtccgtcggc gagcccagga tgatccgcga
     3421 gccctcggcc agcgtgtatt cctgcgggtc cttccggttc ggcgcataga ccagcaccga
     3481 cgggtgcagc tcccggtcgg ggtcggtctg caccacgagg gagcggaggc ccgtggactc
     3541 atcgagctcc tcccgcagcg tgacgcccgg caccaggtcc ttccaccgga ggtcgccgtc
     3601 gatcttgagg atgctcgggt cgttgtacgg atcccaggtg tagaggatcg tctgccgccc
     3661 ctgggccgcg tcgtgctcct tgacctgcag gagcgcgcct tcgggcaccg ggtagcggtt
     3721 gagcacccgc ttgtggtcct tcgggtcggt gacgaggagg ccttccttgg tctcgtcgga
     3781 ggccgactcg tcctcgcggg tgaggacgat ccggatggac cccttgagcc cgtcctcgta
     3841 ctcgacgggc acgtcggtcc actccaggcc cttggtgtag tgcacggtgc cgtcggcccg
     3901 cgcccggcgc tcggcgtcct ccgcgatgcg ggacgacgtg ccgccgatgt ggaaggtccg
     3961 gagcgtcagc tgcgtgcccg gctcgccgat cgactgcgcg gccaggatgc cgacggcctc
     4021 gcccatgtcc accatgtcca tcgtggcgag gttgcggccg tagcacatcc ggcacacgcc
     4081 gcggcgcgct tcgcaggtga ggacggagcg gatcttgacc ttctcgatcc cggcgtcctc
     4141 gatggccacc gatgcgtcct cgtcgatgag ctggccggcc tgcaccagca gcttcgggtc
     4201 gccgaattcg tcgagctcgt gcgggtcgta gacctcctcc gccgcgacgc tccccatcac
     4261 gcgctcgcgg aggccctcga tgatgtcctc gccctccttg agggcggtca tctcgaggcc
     4321 gagggtggtg ccgcagtcct cctcggtgat cgtgacgtcc tgcgccacgt ccaccagccg
     4381 gcgggtcagg tagccggcat cggcggtctt gagcgccgtg tcggccaggc ccttccgggc
     4441 gccgtgcgtc gagatgaagt actcgagcac cgtgagcccc tcacggaagt tcgacttgat
     4501 cgggctctcg atgatctcgc cgatgccgcc ggtgagcttc ttctgcggct tggccatcag
     4561 gccgcgcatg ccggccagct gccggatctg gtcgcggctg ccgcgggagc cggaatcgaa
     4621 catcatgaac accgggttga acccgccgcg ggagtcgcgc atcccgttga ccatcgcctc
     4681 ggcgacgtcg ttgttggcgt gggtccaggc gtcgatcacc ttgttgtacc gctcgccgaa
     4741 ggtgatctgc ccggtgttgt aggcccgctg gaagcgctgc acgcgctggt cggcctcgcc
     4801 gaggagcttg gccttctccg ccgggatgtg caggtcctcg atgccgatcg acaccccgcc
     4861 gagcgtggcg aaccggaacc cgaactcctt cagccggtcg aggaagagca ccgtctccgc
     4921 caggcccgcg cggcggtagc cctggagcac cagctccgag agcgccttct tcttcatcac
     4981 ctcgtcgcgg aagccgagct ccgccaccag ctggcgcggg aggatgctgt tgaacagcac
     5041 ccggcccgcg gtggtggcga tccaccgcgg cgcggcgtcg gccgcctcgc gcggcggggt
     5101 gtaccagaag tggatcgcgt cgtggtgggt gatggccccg tgcgccagcg ccatctccag
     5161 ttcggcgagg gtgccgtacc acttcgcgtc cttccagagc gccttgacgg cctcctggtt
     5221 cttcccggcc ttgcggacct gctcctcgaa cccgggcggc agcttggtga gccagtagct
     5281 ccccagcacc atgtcctgcg acggctccgc caccggccgg ccgtcggacg gcttgaggat
     5341 gttgttcgac gagatcatca gcagccgcgc ctcgagctgc gcctcgaagg agagcggcac
     5401 gtggacggcc atctggtcgc cgtcgaagtc ggcgttgaac gccgcgcaga cgagcgggtg
     5461 gatgcggatg gccttgccct cgaccagcac cggctcgaac gcctggatgc ccaggcggtg
     5521 gagggtcggc gcccggttga ggagcaccgg gtggtcctgg atgatctcct cgaggatctc
     5581 gtacacctcg gggctctcgc gctcgacgat cttcttggcg cgcttgaccg tctcggcgat
     5641 gcccttctcc accagcttgt ggatgatgaa gggcttgaag agctcgaccg ccatcgtctt
     5701 gggcaggccg cactggtgga gccggagctc cgggcccacc acgatgaccg accggcccga
     5761 gtagtccacg cgcttgccga gcaggttctg ccggaagcgc ccctgcttgc ccttgagcat
     5821 gtcgctcagg gacttgagcg ggcgcttgcc ccggccgcgg atggccttgg agcggcggcc
     5881 gttgtcgaac agcgcgtcga ccgcctcctg cagcatccgc ttctcgttcc ggaggatgac
     5941 ctccggtgcg cggtgctgga tgagcttctg cagccggttg ttgcggttga tcacccggcg
     6001 gtagaggtcg ttgaggtccg aggtcgcgaa gcgcccgccg tcgagcggca ccagcggccg
     6061 caggtcgggc gggatgacgg gcacgacatc gaggatcatc cacgccgggt tgttgggcag
     6121 gtcgccctgg tcgcccgagc tgaggaacgc gtcgacgatc ttgagccgct tgagcatctg
     6181 cttcttgcgg tgctggctgg tctcgtcggc cacggtggcc cggagctcct cggccagctt
     6241 cttcacgtcg atgcggctga gcagctcgcg caccgccggg gcgccgatgt cggcgcggaa
     6301 ggcgtggtcg ccgtcctgct tggccttctg ccggagggcg aggtactcct cctcgtccag
     6361 cagctggttg gcctcgacgt cctgctcgcc cggctcgatg acgatgtagc tcgagtagta
     6421 gatgacccgc tcgaggtcgc gcagcgtcac gttgagcagg ttgcccatcg ggctcggcag
     6481 cgtcttgaag aaccagatat gcg
//
LOCUS       DASUAP010000142         4562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_153890, whole genome shotgun sequence.
ACCESSION   DASUAP010000142 DASUAP010000000
VERSION     DASUAP010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153890"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFU45_03380"
     CDS             complement(<1..616)
                     /locus_tag="VFU45_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5358139.1"
                     /translation="MLDRILDDGMSAERVIASMRRDFLLDLSDGFVYDCLAWQVRRLD
                     LAEHRRWVLDRFSGTLCIDELHLGRMTLLLATDPIQDLPVAFALVDSNDQPHMRRFLT
                     NLDRWGFRPEVVVTDGSNLYPTILAELWPDARHQLCVFHVLKDLHKLVLDAVRRMRRA
                     MSRRGNRGRKRKPGRPPKSRAKRRKLSNKEKSAFVFKHRWLIVKR"
     gene            867..1448
                     /locus_tag="VFU45_03385"
     CDS             867..1448
                     /locus_tag="VFU45_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358140.1"
                     /translation="MYHSLPSDARFWSFLVSVDEDLADTTRKRGCPCGGRLHCANYPR
                     KPRGGPQSLPETYQYRLSFCCQRDGCRKRVTPPSVRFLGRRVYLAVVVILVAAMRHGT
                     TPRRLRELSAHFGADRRTIARWLVFWRELFPNTPFWKLARARLAPDVDLTALPRAFLD
                     AFLRGPDDSDGWGRLLRFLAPITIPGTPRIEFS"
     gene            1558..3030
                     /locus_tag="VFU45_03390"
     CDS             1558..3030
                     /locus_tag="VFU45_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEU5358141.1"
                     /translation="MTPAHPSAAHLWARFRFSVVGVLLSSPPPRGELKAAIRSLAART
                     WTHPTTGRDVRFSAVTIERWYYTARHGGDDPVAVLRRAVRKDCGKVSLATALAERLSL
                     QYHQHPHWSYQLHYDNLAALVQAEPALGPLRSYATVRRSMKAHGWVPRPRPHPKQRPG
                     EALAEQRRQTREIRSYEAAYVGALWHLDFHHGSLKVLTPGGQWLRPIALGILDDHSRL
                     CCHIQWYLSETAADLVHGLSQAIQKRGLPRALLTDNGPAMVADEVTEGLLRLGIVHER
                     TLPYSPYQNGKQEAFWGTLEGRLMTMLEGVAQLTLASLNEATQAWMEIEYNRATHREI
                     SGPPVERFARAPDVLRTSPSSEALRDAFRLETGRIQRRSDGTISLEGVRFEIPARYRH
                     FREVAVRYARWDLGRVDLVDRKSGTLLAPIYPLDRAANADGRRAFVAADRGDVPPEGA
                     PCRDGAVPPLLKAILAEYSAPGLPPAYLPRTPPSGTGGAS"
     gene            3027..3872
                     /locus_tag="VFU45_03395"
     CDS             3027..3872
                     /locus_tag="VFU45_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5358142.1"
                     /translation="MNAKKLLALWGLKWDPFSPELPGDALRMTPAIEHFAWRVEQLAQ
                     EGGFALITGESGTGKSVALRIVAGRLSALRDVTVGVIERPQSRAADFYRELGDLFAVK
                     LQPHNRWCGFKALRERWKAHMASARLRPVLLIDEAQEMSPDVLGELRILSSADFDATA
                     LLTVVLSGDGRLSELLRREDLVPLSSRIRARLVTEPAAREELLELLRHALSMAGNTSL
                     MTAELMDALVDHSGGNYRSLMIMGGDLLAYGMAHDLAQLDERCYLEVFQPRGARAVPR
                     KRVRA"
     gene            4033..4347
                     /locus_tag="VFU45_03400"
     CDS             4033..4347
                     /locus_tag="VFU45_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEU5358143.1"
                     /translation="MAGVDFDRVRSEITMEQVLDLLGFEPVSRSGVQWYGVCPLHESR
                     SGNRRGFSVNVALGRYYCHGCRSRGNQLELWASATNRPLYQAAIDLCDRLGRDVPWIR
                     RW"
BASE COUNT          688 a         1616 c         1542 g          716 t
ORIGIN      
        1 gtcgcttcac gatcaaccag cggtgtttga acacgaatgc cgatttctcc ttgttgctga
       61 gcttgcggcg cttcgctcgc gatttgggag gccgtcccgg cttccgcttg cggccgcgat
      121 tgccccgccg ggacatcgct cgccgcatcc gcctcaccgc gtccaggacc agcttgtgca
      181 ggtccttcaa gacatggaac acgcacaact gatggcgcgc gtcgggccac aattcggcca
      241 ggatcgtcgg atagaggttg gagccgtcgg tcacgacgac ctcgggccgg aatccccacc
      301 ggtcgaggtt cgtgaggaac cgtcgcatgt gaggctggtc gttgctgtcg accaaggcaa
      361 aggccacggg cagatcctgg atcgggtcgg tggccagcag caaggtcatg cgaccgaggt
      421 gcagttcgtc gatgcagagg gtgccgctga agcggtccag gacccagcgg cggtgctcgg
      481 cgagatcgag ccgtcggacc tgccacgcga ggcagtcgta gacgaacccg tcggagaggt
      541 ccaggaggaa gtcccgccgc atcgaggcga tgacccgctc ggcgctcatg ccgtcgtcga
      601 ggatgcggtc gagcaccgcc tggcggacct tgttgtcgta gagggcccgc ggctcgaggc
      661 ccggcggtgt ggtgcggaac gtggcgcagc agccgcatcg ggcgcggtac ttgagcgtgg
      721 ccaggataat cgacgggaga tccgcgattc ctgagcaact tcggtgaggc tgccaagaaa
      781 aatcccctcg gagagcctga tcactggatc acgctccccg aagggtgtac tccgtagggt
      841 gtcacccaac cacggagtac tccaccatgt atcacagcct gccttccgac gccagattct
      901 ggtcgttcct cgtctccgtc gacgaagatc tcgccgacac cacgcgcaaa cggggatgtc
      961 cctgtggtgg ccgcctgcac tgcgccaact acccccgtaa gccgcggggc ggaccccaat
     1021 ccctccccga gacttatcag tatcgactga gcttctgctg ccagcgcgac ggctgccgca
     1081 agcgagtcac ccccccctcg gtccgcttcc tgggccggag ggtttacctc gccgtcgtcg
     1141 tcatcctggt cgccgccatg cggcacggga cgactccgcg ccggctccgc gagctttccg
     1201 cccacttcgg ggccgaccgc cgcaccatcg cgcgctggct ggtcttctgg cgcgaactct
     1261 tccccaacac ccccttctgg aagctcgccc gcgcccgcct ggcacccgat gtcgatctca
     1321 cggccctccc acgcgccttc ctcgacgcct tcctccgcgg gcctgatgac tccgacggct
     1381 gggggcgcct gcttcgcttc ctcgccccga tcacgatccc gggaaccccg cggatcgagt
     1441 tctcctgagg ggcggccacg atccgcagac aatgctcctt tcgaccctcg gccggccggc
     1501 cgtagaacgg atgacacggc cctttccgac gcttcaccgg cctcccgagg aaccctcatg
     1561 accccggcac acccttcggc cgcccacctg tgggcgcgct ttcgcttctc cgtcgtcggc
     1621 gtgctgttga gctcgccccc gccgcgcggc gaactcaagg ccgccatccg atccctggcc
     1681 gccaggacct ggacccaccc caccaccggg cgcgacgtcc gcttctcggc cgtcaccatc
     1741 gagcgctggt actacaccgc acggcacggc ggcgacgacc ccgtcgccgt cttgcgccgc
     1801 gccgtgcgca aggactgcgg caaggtctcc ctcgccacgg ccctggccga gcggctctcc
     1861 ctccagtacc accagcaccc gcactggagc taccaactcc actacgacaa cctcgccgcg
     1921 ctggtccagg ccgagcccgc gctcgggccg ctccggtcct atgccacggt gcgccgctcc
     1981 atgaaggcgc acggctgggt ccccaggccg cggccacatc cgaagcagcg ccccggggaa
     2041 gcacttgccg agcagaggcg ccagacccgg gagatccgca gctacgaggc cgcctacgtc
     2101 ggggcgctgt ggcatctcga tttccaccac ggctcgctca aggtgctcac ccccgggggc
     2161 cagtggctcc gcccgatcgc cctgggcatc ctcgacgacc actcgcgact ctgctgccac
     2221 atccagtggt atctctccga gaccgccgcg gatctcgtgc acggcctctc ccaggcgatc
     2281 cagaagcgcg gcctgccgcg ggccctcctg accgacaacg gcccggcgat ggtggccgac
     2341 gaggtcaccg aggggctgct ccgcctgggg atcgtccacg agcggacgct cccctacagc
     2401 ccctaccaga acggcaagca agaggcgttc tgggggaccc tcgaggggcg gctgatgacg
     2461 atgctcgagg gggtcgccca gctcacgctg gcgtcgctca acgaggcgac ccaggcgtgg
     2521 atggagatcg agtacaaccg cgcgacacac cgcgagatct ccggtccgcc ggtcgagcgc
     2581 ttcgcccgcg cgcccgacgt gctccgcacg agcccctcga gcgaggcgct gcgggacgcg
     2641 ttccgcctgg agaccggccg gatccagcgc cgcagcgatg gcacgatctc gctggagggg
     2701 gtgcgattcg agatcccggc gcggtatcgt cacttccgcg aggtggccgt gcgctacgcc
     2761 cgctgggacc tcggccgggt cgacctggtc gaccgcaaga gcgggaccct gctcgccccc
     2821 atctaccccc tcgaccgggc cgccaacgcc gacggccgcc gcgccttcgt ggcggccgac
     2881 cgcggggacg tgccgccgga gggcgcgccc tgccgcgacg gcgcggtgcc gccgctcctg
     2941 aaggcgatcc tcgccgagta ctcggccccc gggctgccgc cggcctatct gcccaggaca
     3001 ccgccgtcgg ggaccggagg tgcctcatga acgccaagaa gctgctggcg ctgtggggcc
     3061 tgaaatggga cccgttctcg cccgagctcc ccggcgatgc cctgcggatg acgcccgcga
     3121 tcgagcactt cgcctggcgc gtcgaacagc tggcccagga ggggggcttc gccctgatca
     3181 cgggcgagtc ggggaccggg aaatcggtgg cgttgcggat cgtcgccggg cggctttctg
     3241 ccctgcggga cgtcacggtg ggcgtgatcg agcggcccca gtcgagggcc gccgacttct
     3301 atcgcgagct gggcgacctg ttcgcggtga agctccagcc gcacaaccga tggtgcggct
     3361 tcaaggcgct gcgcgagcgc tggaaggccc acatggcctc ggcacggctc cggccggtgc
     3421 tcctgatcga cgaggcccag gagatgagtc ccgacgtcct gggcgagctg cggatcctct
     3481 ccagtgccga cttcgatgcc acggcgctgc tgacggtggt cctctcgggg gacgggcggc
     3541 tgtcggagct gctgcggcgg gaggacctcg tgccgctctc gagtcggatc cgggcgcggc
     3601 tggtcaccga gccggccgcg cgcgaggagc tgctggagct gctccgccac gcgctgtcga
     3661 tggccggcaa cacgtcgctg atgacggccg agctgatgga cgcgctggtc gatcacagcg
     3721 gcggcaacta ccggtcgctg atgatcatgg ggggcgatct cctggcctac ggcatggcgc
     3781 acgacctggc gcagctcgac gagcggtgct acctggaggt gtttcagccg cggggcgcgc
     3841 gcgccgtgcc gaggaagagg gtgagggcgt gaggatggcc gcgggtgccg agcgaacgcc
     3901 ccccgtcgcg cggggggaga tcgccggcac cgcggcggat cgatcgatct gtggagggtc
     3961 gtcccagttg cccggagaga gcgatcgggg acggaacgat cctcccgtgc cgtagccgag
     4021 cgaggaacca tcatggccgg tgtcgacttc gacagggtcc gtagcgagat cacgatggag
     4081 caggtgttgg acctgctcgg cttcgagccc gtgagccgat cgggggtgca atggtacggg
     4141 gtctgtccgt tgcatgagtc gcggtcgggg aatcgccgcg gcttctcggt gaacgtggcg
     4201 ctggggcggt actattgcca cgggtgccgc agccgtggta accagctcga actctgggcg
     4261 tcggccacga atcggccgct gtaccaggcc gcgatcgatc tttgtgatcg tctgggccgc
     4321 gacgtcccct ggatcagacg ttggtgaggc ccgcccgaac ctcgtgccac cgcacggcgg
     4381 gagaccggcg ccaccgaacg aaacgggata gagaagaggc caccggtact caggagatcg
     4441 tcaaggcgag ggagcccatc gatgatctcg atcggcggtg cgtcaattac tctgaccact
     4501 ctcacgcggt tttccagatc cggagtgtga gatcgtcgct tgtcgtcgca agctctcggt
     4561 cg
//
LOCUS       DASUAP010000143         5236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1298173, whole genome shotgun sequence.
ACCESSION   DASUAP010000143 DASUAP010000000
VERSION     DASUAP010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298173"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFU45_03405"
     CDS             <1..314
                     /locus_tag="VFU45_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358144.1"
                     /translation="VRATEPTCDVTIADSARLIVRFRQPPELALPPLAPRRGDPSRAV
                     RVTDLVASGDSVTFTLEGPAGSTVRLARVDAPDSVAVRFPEPGDSVDRYSRTHVRLGR
                     R"
     gene            439..2679
                     /locus_tag="VFU45_03410"
     CDS             439..2679
                     /locus_tag="VFU45_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5358145.1"
                     /translation="MRALAGLAVVAFAGPVAAQNVADLQVTPEALTLKVGETKSLFPQ
                     AYDANQNIVVARFQYFSQKPGVARVDGEGRVTGISPGSTQITVRAGRKSAPVSVLVSP
                     GDNPPPAEPAPAPRNAAPQAITLPPAPAGTQQIVIDPATVYLLQSESAHLVARALAAD
                     GSVLGQMRVIWRSLTPGVVSVIDSLNGDVVGAAPGMGTIQATGPAGLIATAPVQVVAT
                     STAFESRYPRMVLSPDQIDTMEMVVPSQSGRRIETSLTYSSSNPGVVLVGPTGLLQAQ
                     SPGQSDITVNGYFQSKHVTVIVHRPISFFLTDPPSGGQVTVPLDGFRSLTAKAEAADS
                     TLIPEAPLTWKVADTALARYDTAAGRIIGRKEGLTTLTLTVRGYVPKVWGLRIVPGGI
                     GLDHHTLGLVIGTSADLSVELRDAQGQGYGPAPEVTWISDHPDVAKVVDGKVTAVGPG
                     SAFIIASVPWGKADTAKVFSTGDLLLASDRASAGISGLYEATLHAPANLLRLLTAAFP
                     ATDPALSPDRMRIAYSARGDGTDFDIWVMDADGRNAHPLTNDSAAEVHPSWTPDGKAL
                     VYSIIGRDRDQLWLNTFRDRRPLTIEKTSASAPSVSPDGKWVAYVGGKDRRKPDVFLR
                     GLTRDTIIQVTETKQKETAVGWFPNGDLAWVTEATDGSKGYEVLRMTLGSTQRTVVAT
                     SAYPILDFAISRDGGTIAYVTEEPNPGNKQQRTKTVLYLGAVTPGSAPQAVALPTSET
                     VGSPAF"
     gene            2702..3742
                     /locus_tag="VFU45_03415"
     CDS             2702..3742
                     /locus_tag="VFU45_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5358146.1"
                     /translation="MRALTLTAAGGIPSLLLQDVPPPPAPAPGWVVVRVRMAALNHLD
                     LFVAEGLPGVRPPFPFPVGADGAGTVAAVGPGVTAVRPGDEVLIDPGVSCGTCRACRA
                     GDEPLCERFGILGEHLPGTLAEFVAVPAVNLAPRPPEMPWPQSAAFSLATLTAWRMLV
                     TRARLAAGEAVLVWGAGGGVAQAAIAIAVHLGARVIATSSRAEGLERARALGAAVLVD
                     HAREDIVAAVKAATEGRGADVVVDSVGRDTWERTLRAVARGGRIVTCGGTSGPELALD
                     VRRLFWRQWSILGSTMGSHAEYRAVTGLAQAGKLWPVVDAVVPLADAAAAFERLAAGR
                     QTGKLVIEVSHE"
     gene            3735..4454
                     /locus_tag="VFU45_03420"
     CDS             3735..4454
                     /locus_tag="VFU45_03420"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358147.1"
                     /translation="MNELITRLHDGLDALVGYGPAILAFLAIILAGLLFARQLERWID
                     GLLARINFNRMAEAGGISEAVGRVQKGLDPVHAVGKLVFWLVMLVAILLASAALGLES
                     ISQMFGMLLAFIPTLVTAIVIIILGIIVGEFVRGVITASAGSVEGVPTFAKLAKAVVV
                     LMAVFMAISQMGVAAEIVDAAFVLIVGAVALAAGLAFGLGNRELAGEITRRWYEAGRR
                     RRMPPANPASEGGAKDAPPPA"
     gene            complement(4528..>5236)
                     /locus_tag="VFU45_03425"
     CDS             complement(4528..>5236)
                     /locus_tag="VFU45_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358148.1"
                     /translation="GGKLALTIAEPAALGGALPTGRDADGWLYAEVRGGQLDSGSVVR
                     WRVGAARPDTVARLAPYTLQEVNGPQGRRLARQIYSGNDIWGVFPSGALWVARVTKNR
                     LDQRDTAGTWRKGPALPDPIYPVTRFDKDYYIQGFPADQRPMASTLPFAPVKAPFDAA
                     FVEDDSLVWLQASRSLTDSIRTYKVLDRSGGYAQEVRLKNARRILGAHGGTILVAEQL
                     EPGKGFKLLRYPSHGIP"
BASE COUNT          693 a         1993 c         1790 g          760 t
ORIGIN      
        1 ccgtgcgcgc caccgaaccg acctgcgacg tgaccatcgc cgattcggcg cggctgatcg
       61 tccgcttccg ccagccgccg gagctcgcgc tgccgcccct ggcgccccgc cggggcgatc
      121 cctcccgcgc cgtgcgggtc accgacctcg tggcgagcgg ggattcggtc accttcaccc
      181 tcgaaggacc ggcgggctcc accgtgcgcc ttgctcgggt cgacgcaccc gactccgtcg
      241 ccgtgcgctt cccggaaccc ggggactcgg tcgaccggta ttcccgcacc catgtccggc
      301 ttggccgccg ctgaggcccg ccgtccgaca gggtgcatca agttgccggg tcgtgccgga
      361 cggcccatat ttggctcagg aaggtcatcc gatctctcca gccccgtagc cgacatgcca
      421 cttgctcgtc gaatcctcgt gcgcgcgctc gcgggcctcg cggtcgtggc ctttgccggc
      481 ccggtcgcgg cccagaacgt cgcggacctc caggtcacgc cggaggcgct gacgctcaag
      541 gtcggtgaga ccaagtcgct ctttccccag gcgtacgacg ccaaccagaa catcgtcgtc
      601 gcccggttcc agtatttcag ccagaagccc ggcgtggcgc gggtcgacgg cgagggccgg
      661 gtcaccggta tctcgcccgg gagcacccag atcaccgtcc gtgcggggcg caagtcggcc
      721 ccggtctcgg tgctggtgag tccgggcgac aacccgcccc ccgccgagcc cgccccggcg
      781 ccgcggaacg ccgcgcctca ggccatcacc ctgccgccag cccccgccgg cacccagcag
      841 atcgtgatcg accccgccac cgtctacctg ctgcagtccg aaagcgctca cctcgtggcg
      901 cgcgccctcg ccgccgatgg ctcggtgctc ggccagatgc gggtcatctg gcgctcgctc
      961 acgcccggcg tcgtgagcgt catcgactcc ctcaacggcg atgtcgtcgg cgccgcgccg
     1021 gggatgggca ccatccaggc caccggacct gccggcctca tcgccacagc gccggtgcag
     1081 gtggtggcca cgagcaccgc cttcgaatcg cgctatccgc gcatggtgct ctcgcccgac
     1141 cagatcgaca cgatggagat ggtcgtgccg agccagagcg ggcgcaggat cgagacgtcg
     1201 ctcacctact cgtcgtccaa tccgggcgtg gtgctcgtgg gccccaccgg gctcctgcag
     1261 gcccagagcc cgggccagag cgacatcacc gtcaacggct acttccagtc caagcacgtg
     1321 acggtcatcg tgcaccggcc gatctccttc ttcctgaccg acccgccctc cgggggccag
     1381 gtcacggtgc cgctcgacgg cttccgctcc ctcaccgcca aggccgaggc ggccgactcg
     1441 acgctgatcc ccgaagcacc gctcacctgg aaggtggccg acacggcgct cgcccggtac
     1501 gacacggccg ccggccggat catcgggcgc aaggaggggc tcaccaccct cacgctcacg
     1561 gtgcggggat acgtgcccaa ggtctggggc cttcgcatcg ttcccggcgg catcggcctc
     1621 gaccaccaca cgctgggcct cgtcatcggc acgagcgccg acctctccgt cgagctgcgc
     1681 gacgcgcagg gccaggggta cggccccgcg ccggaggtca cctggatcag cgaccatccc
     1741 gacgtggcca aggtcgtcga cgggaaggtg accgcggtgg ggccgggaag cgccttcatc
     1801 atcgcgtcgg tgccgtgggg caaggccgac acggccaagg tcttctccac cggcgacctc
     1861 ctgctcgcct ccgaccgcgc gagcgccggc atctccggcc tgtatgaggc gacgctccac
     1921 gcgccggcca acctcctccg gctcctcacc gcggcgttcc cggccaccga tccggcgctc
     1981 tcgcccgacc ggatgcgcat cgcctattcg gcccgcggcg acggcaccga cttcgacatc
     2041 tgggtgatgg acgccgacgg acgcaacgcg cacccgctca ccaacgacag cgccgccgag
     2101 gtccacccgt cgtggacgcc ggacggcaag gcgctggtct attccatcat cggcagggac
     2161 cgcgaccagc tctggctgaa taccttccgc gaccggcgcc cgctcacgat cgagaagacc
     2221 tccgcgagcg cgccatcggt ctcccccgac ggcaagtggg tggcgtacgt cggcgggaag
     2281 gaccgccgga agcccgacgt cttcctccgc ggcctgaccc gcgacaccat catccaggtc
     2341 accgaaacca agcagaagga gacggcggtc ggctggttcc ccaacggcga cctggcctgg
     2401 gtcaccgagg ccaccgacgg cagcaagggc tacgaggtgc tgcggatgac gctcggctcc
     2461 acccagcgca ccgtggtggc cacctcggcc tatccgatcc tcgacttcgc catctcccgc
     2521 gacggcggca ccattgcgta cgtcacggag gagccgaacc cgggcaacaa gcagcagcgg
     2581 accaagaccg tcctctacct cggcgccgtc acgccgggca gcgcgcccca ggccgtggcc
     2641 ctcccgacct ccgagaccgt cggctccccc gcgttctagc gggcccgcgg atccgccgct
     2701 gatgcgcgcc ctcacgctca ccgccgcggg cggcatcccg tcgctcctgc tccaggacgt
     2761 gccgccgccc cccgcccccg ccccgggatg ggtggtggtg cgcgtccgga tggcggcgct
     2821 caaccacctc gacctcttcg tcgcagaggg actgcccggc gtccgcccac cgttcccctt
     2881 cccggtcggc gcggacgggg cgggcaccgt cgccgccgtc ggtcccgggg tgaccgctgt
     2941 ccgccccggc gacgaggtgc tgatcgaccc cggcgtctcc tgcggcacgt gccgcgcctg
     3001 ccgcgccggc gatgagccgc tctgcgagcg tttcggcatc ctgggcgagc accttcccgg
     3061 caccctggcc gagttcgtcg cggtcccggc ggtgaatctc gcgccccggc cgccggagat
     3121 gccgtggccc cagtcggcgg cgttctccct cgccacgctc accgcctggc ggatgctcgt
     3181 cacgcgggcg cggctggccg ccggcgaggc ggtgctggtg tggggtgccg ggggcggcgt
     3241 cgcccaggcg gcgatcgcca tcgcggtgca cctgggcgcc cgtgtcatcg ccaccagctc
     3301 ccgcgccgag gggctggagc gcgcccgcgc cctcggcgcc gccgtgctgg tggaccacgc
     3361 gcgcgaagac atcgttgccg cggtcaaggc ggccaccgag gggcgcggcg ccgacgtggt
     3421 ggtcgacagc gtcggccggg acacgtggga acgcaccctg cgtgcggtgg cgcggggcgg
     3481 gcgcatcgtc acctgcggcg gcaccagcgg ccccgagctc gcgctcgacg tgcgccggct
     3541 cttctggcgg cagtggagca tcctcggttc cacgatgggg agccacgccg agtaccgggc
     3601 cgtgaccggg ctggcccagg cggggaagct ctggcccgtg gtcgacgcgg tggtgccgct
     3661 cgccgacgcg gcggcggcgt tcgagcggct tgcggcgggc cgccagaccg gcaaactcgt
     3721 catcgaggtg tcccatgaat gaactgatca cccggctgca cgacggcctc gatgcgctgg
     3781 tgggctatgg tcccgccatc ctcgcgttcc tggccatcat cctggccggg ctcctctttg
     3841 cccggcagct cgagcgctgg atcgacgggc tcctggcccg catcaacttc aaccggatgg
     3901 cggaggcggg cgggatctcc gaggcggtgg gacgggtaca gaaggggctc gacccggtcc
     3961 atgccgtcgg gaagctggtg ttctggctcg tgatgctcgt ggccatcctg ctcgcgagcg
     4021 cggcgctcgg gctcgagagc atcagccaga tgttcgggat gctcctcgcg ttcatcccga
     4081 cgctcgtgac cgccatcgtg atcatcatcc tcggcatcat cgtcggcgag ttcgtgcggg
     4141 gcgtgatcac cgcgtccgcc gggagcgtgg agggggtccc gaccttcgcc aagcttgcga
     4201 aggcggtggt ggtgctgatg gcggtgttca tggccatcag ccagatgggc gtcgccgccg
     4261 agatcgtcga tgccgccttc gtcctcatcg tcggcgcggt ggcgctcgcc gcgggtctcg
     4321 ccttcgggct cggcaatcgc gagctggccg gggagatcac ccgccggtgg tacgaggccg
     4381 ggcgccgccg ccgcatgccg cccgccaacc ccgcgtccga gggcggggcc aaggacgcgc
     4441 cgccgccggc ctgaggagtg caccgtcgca acaagaagcc ccggcattcg gccggggctt
     4501 cgttgcgtcc agggggtggc atgccgctca cgggatcccg tgcgatggat agcgcaggag
     4561 cttgaacccc ttgcccggct cgagctgctc ggccaccagg atcgtccccc cgtgggcacc
     4621 gaggatccgc cgggcgttct tgagccggac ctcctgcgcg tagccacccg aacggtcgag
     4681 gaccttgtag gtccggatcg agtcggtcag gctccggctc gcctgcagcc agacgaggct
     4741 gtcgtcctcg acgaatgcgg catcgaacgg ggccttgacc ggcgcgaacg ggagcgtcga
     4801 cgccatcggg cgctggtccg cgggaaagcc ctggatgtag tagtccttgt cgaagcgcgt
     4861 caccgggtag atcgggtcgg ggagcgccgg acccttccgc cacgtgccgg cggtgtcgcg
     4921 ctggtcgagg cggttcttgg tcacccgcgc cacccagagc gctcccgaag ggaacacccc
     4981 ccagatgtcg ttcccgctgt agatctgccg cgccagccgt cgtccctggg gtccattcac
     5041 ttcctgcagc gtgtacgggg ccaggcgggc caccgtgtcg gggcgcgccg cgccgacccg
     5101 ccagcgcacc acgctccccg agtcgagctg cccgccccgc acctcagcgt aaagccaccc
     5161 gtcggcgtcg cgtccggtcg gcagggcacc acccagtgcg gccggttcgg cgatggtcag
     5221 ggcgagcttc ccgccc
//
LOCUS       DASUAP010000144         9566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1202946, whole genome shotgun sequence.
ACCESSION   DASUAP010000144 DASUAP010000000
VERSION     DASUAP010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202946"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFU45_03430"
     CDS             complement(<1..279)
                     /locus_tag="VFU45_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEU5358149.1"
                     /translation="MTHDLPLLLYDGDCGFCARQVRFLLRHDRRKALRFAPLGGPTAR
                     GVLERHPELAGVDSLMWVDDLGGAQERVRVRSEAVLAVAAYLGGLWRLA"
     gene            complement(388..624)
                     /locus_tag="VFU45_03435"
     CDS             complement(388..624)
                     /locus_tag="VFU45_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEU5358150.1"
                     /translation="MGTRVLRQHGIDVIFFGNADSTVDSTRIIVRRGDPARAAAIRKA
                     LGGGKVSVAIDTLRRVDATVVLGGDFRGPWEVHP"
     gene            complement(585..824)
                     /locus_tag="VFU45_03440"
     CDS             complement(585..824)
                     /locus_tag="VFU45_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358151.1"
                     /translation="MELARHARLKRTILGVVGVALTLALLGALVRGGRGDIVPGHAWA
                     VPSGPDRLIVEVMNGTPTPGSRGWGRGSSASTAST"
     gene            complement(797..1345)
                     /locus_tag="VFU45_03445"
     CDS             complement(797..1345)
                     /locus_tag="VFU45_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5358152.1"
                     /translation="MAETGLLPPWAAVSAERRAHIERVAALMDGWAEALGLPAAERQR
                     WRRAAWLHDALRDAPEAELVRWAGSEALPVELLHGPAAANRAEREGMVDRGVLDAVRW
                     HSVGSDRWDAVGRALYSADFLEPGRSFDREARAALADRFPADPDGVFRDVARARLLWV
                     IRSGWPLPDTTVRMWNSLATPA"
     gene            complement(1355..1726)
                     /gene="panD"
                     /locus_tag="VFU45_03450"
     CDS             complement(1355..1726)
                     /gene="panD"
                     /locus_tag="VFU45_03450"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEU5358153.1"
                     /translation="MQRHLMKSKIHRATITSADLHYEGSLTVDQDLLDAADLVEFEDV
                     QVVNVNNGQRFNTYVIPGPRGSGVIQLNGAAARLGLPGDLVIIIAYGVVDEAEVERFQ
                     PRIVFVDGHNRVVRPPLRAEH"
     gene            complement(1740..2582)
                     /gene="panC"
                     /locus_tag="VFU45_03455"
     CDS             complement(1740..2582)
                     /gene="panC"
                     /locus_tag="VFU45_03455"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU5358154.1"
                     /translation="MIEITAIAELRRWLGDERRAGRRIGFVPTMGALHEGHLRLVDEA
                     RRRADLVVMSIFVNPLQFGPKEDLARYPRDLARDRALASARGVDLLFVPSVEAMYPPG
                     AETRVVPGAAAERWEGAVRPGHFAGVLTVVAKLFHLVEPDLACFGQKDIQQFTLIRKM
                     VRDLDLPVELVMVPTVRDADGLALSSRNAYLSADERRAALALPAALDAAVAAWRGGER
                     SGKRLEVIVGERLAGSERVVADYIAVVEPDGLTPAGTAGAGTIVAIAARVGTTRLIDN
                     VILG"
     gene            complement(2620..3435)
                     /gene="panB"
                     /locus_tag="VFU45_03460"
     CDS             complement(2620..3435)
                     /gene="panB"
                     /locus_tag="VFU45_03460"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU5358155.1"
                     /translation="MPAEKTRPRAIPDLAAWKQDGRKLAMLTCYDALFARLLEASGVD
                     LLLVGDSLHQVLGGHETTLGATLDAMIYHAAAVRRGARETLVFIDLPFLTYQVSVAEA
                     VRNAGRAMQEANVHGVKLEGGRHMAPTIHALTDVGIPVMAHLGLTPQSVHQLGGYRVQ
                     GRDQEGAERLLADARAVEEAGACAVVLELVPSKVAKKVTAAVGIPTIGIGAGPHCDGQ
                     VLVLHDMLGLNEGFDPKFLKKYGALAGATKDAVRQYVAEVRAGKFPDAGHSFE"
     gene            complement(3425..3844)
                     /gene="folK"
                     /locus_tag="VFU45_03465"
     CDS             complement(3425..3844)
                     /gene="folK"
                     /locus_tag="VFU45_03465"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU5358156.1"
                     /translation="MTRPARAWVALGSNLGDRDAHLAQARAAIGALPGTRVLAESRVE
                     ETAPLGDIPQGPYLNQMVLLETELAPRALLHSLLAIERAAGRVRGTRWGPRTLDLDIV
                     RYDDLTLRGPGLTLPHPALGERDWWQRELKELEAHAG"
     gene            complement(3841..5922)
                     /locus_tag="VFU45_03470"
     CDS             complement(3841..5922)
                     /locus_tag="VFU45_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5358157.1"
                     /translation="MRIPVDSAVRGFTRSLAVVGVVLFPAVLLVDRTWLAHPWGAVAL
                     GFAVLALRASPIQLSKYSYLTQTGVPVLVGAVLLGPGAVSIGLVTGVFLADWALHRKH
                     WLFSWINAGRELAAFVAAFGMHAFVRHMTGAERLSLDYLPAAITLVIGYFFTGRALFY
                     GTLLLRDKLELDERLLILRYEVVAYLLTVAAAVILVGAVETLAPIGWVTVIAVLAVLG
                     VLTKKILEEAIGAEELSKVHAREKIVTSNVSLQESCTQIEALAHRLLDWSDFRIYRVD
                     QGSAVLTYRGVRGRPGRGEPPADSATLRTEAIETGKPVLVTDATRDRRIREPSPHAAS
                     VLIVPLRFGEDTVGTLELEHHKRHSYGAKESATVLAFAGQLATAIHLAELRRPLMETV
                     ERIGVQVRALARATDAVRESAAAVASAAQAIRTELGEQDAFVVGGLEATEQLAKAAQE
                     AARDGATAADASESASETAARNRDAIADALQRLVQVKGFVAESAEQVAALGAVTRKIT
                     GFIGSIRDIADMTDLIALNAAIEAARAGKHGKGFAVVAGEVRQLAEQSTRASHDAGAL
                     VARITAQVSEISEQMRRGEAVVQGVEELSGDAVQALEAIVGSTHAAGGHARRIADAAA
                     GQSKAFKKLRSQIESLALVSYRTREETNSLASQAQEAAQGQIDLERAIRELEGVATYL
                     QALARHFAAGA"
     gene            complement(5919..6710)
                     /gene="trpA"
                     /locus_tag="VFU45_03475"
     CDS             complement(5919..6710)
                     /gene="trpA"
                     /locus_tag="VFU45_03475"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU5358158.1"
                     /translation="MSDATPLSGAWDQLRRRHRTAFIPYLTAGYPDRATSLEALRLAA
                     AVGDIVEVGVPFSDPLADGPIIQRSSFEALAGGMSLAGTLDLVARLDARRPVVLFSYL
                     NPVLRYGVDRLLADAAAAGVSGLLLTDLPAGGDAAVEGAVAGSSLDLIRLVAPTSSVA
                     RIGEAVAGARGFIYLIGRLGVTGTSAAPAADLAASVGRVRAQTSLPVAVGFGVATARQ
                     AGVVARAADGVVVGSALVEALGQQGVAGLERLLGELRGAIDGEAA"
     gene            complement(6703..8220)
                     /locus_tag="VFU45_03480"
     CDS             complement(6703..8220)
                     /locus_tag="VFU45_03480"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEU5358159.1"
                     /translation="MSDDATPPRRTTPTPQRASVAVSSAGTGSGEAALRHGGRGLLLL
                     FYSAHRSLKLYPVENATVQRSLDDLQAATAALLETEHEIEVRVAGDFIFVNSTRLRLE
                     LDNYASFSSILGIFREFDVGAVRIDEGIERREWQAFLSLLLTLTGRPAAAGGTKFSEL
                     TGRMAGAQVEHIHLETTSDSAEAAGDAESQRYAAKRTYSQGVAVTREVMTGARLGRAP
                     SVKKMKRAVQAVVDQVLNNETSLVGLTTIRDYDEYTFTHSVNVCIFSVAIGKRLGLSK
                     QQLYDLGLAALLHDVGKARVPLEILNKTGGLTEDEWRVMQSHPWLGVLTLFGLRGYGE
                     IPYRAAIVAAEHHMKTDLSGYPRVIRSREQGTYSRLVAVADGFDAATSRRSYQTVPIQ
                     PDQVLKEMWDNPRRGYDRVMVKALINLLGVYPVGTVVVLDTLEVGVVAGANPDPTLLN
                     RPLVRLALDADGGIYPPPGQQVDLGDLDEAGHYRRSIVKVTSPDRYGLTVGDYFV"
     gene            complement(8217..>9566)
                     /locus_tag="VFU45_03485"
     CDS             complement(8217..>9566)
                     /locus_tag="VFU45_03485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5358160.1"
                     /translation="SEAEDDLLTLLWEQDFQLIHYTFSEVLTDAAPPAVAGPPVAGFV
                     VGGTDAPVTAEDARARNRDAVTEDAATPRPAGVVRLEDFDSTLYFLDEQEIAYVAREI
                     AKEYDQDLRANVMAFLYDLFEIQTSAEVRDEILMILESFPAYLLAAGDLRSVAQMLRE
                     TKVLALRGRALDEKHRARLEMFARQLSEPNVVAELLRAIDEANAPPAEADLTELFREL
                     RGSALEPVMVWLPKAANAQAREMLERVAVQLAEANPAEILRILGSPESEALGSAITLS
                     GRLRLQPSVPGLGQNLGHADPQVRLASAQALGEIGSPGAFAALERALDDADREVRLYT
                     VRVLGGRAHRGALKTVESVVLAKTAEDRDLTERQAFFEAFALIAGAAALAPLTELLQP
                     SGGLFKRKASPDTRACAVAAIAKIRHPDVRAIVQKATQDKEAVVRNAANRALREVPA"
BASE COUNT         1331 a         3512 c         3439 g         1284 t
ORIGIN      
        1 cgccagacgc cagagcccgc caagatacgc cgccacggcg agcaccgcct ccgaccgtac
       61 acgcacgcgc tcctgggcgc cgcccagatc gtcgacccac atcagagaat ccacccccgc
      121 gagctccgga tgccgctcca gcacgccccg cgcggtcgga ccgccgagcg gcgcgaagcg
      181 gagcgccttc cgacggtcgt gccgcagcag gaaccgcacc tgcctggcgc agaagccgca
      241 gtcgccgtcg tagaggagga gtggcaggtc gtgggtcatg ggtcgcgggt cgcgggtcac
      301 gggtcgaggg tcgcgggtcg cgggtcatgg gccacccgtc ttacgtctca cgtcttacga
      361 ctcacgactc acgacccacg actcgcatca cggatgcacc tcccacggcc ccctgaagtc
      421 cccgcccagc accaccgtcg cgtccacccg gcggagcgtg tcgatggcga cgctcacctt
      481 ccccccgccg agcgccttcc ggatggcggc ggcgcgggca gggtcgccgc ggcggacgat
      541 gatccgggtc gagtcgacag tggagtcggc gttgccgaag aagatcacgt cgatgccgtg
      601 ctggcggagg acccgcgtcc ccacccgcgc gagcccggcg tcggcgtgcc gttcatcact
      661 tcgacgatga ggcggtcggg acccgacggg acggcccacg cgtggcccgg gacgatgtcg
      721 ccccggccgc cgcgcaccag cgcgccgagg agcgccagcg tgagcgccac gccgacgacg
      781 ccgaggatgg tgcgcttcag gcgggcgtgg cgagcgagtt ccacatgcgg accgtggtgt
      841 cgggaagtgg ccagccggac cggatgaccc agaggaggcg ggcgcgcgcc acgtcgcgga
      901 agacgccgtc cggatcggcc gggaaccggt cggccagcgc ggcgcgggcc tcccggtcga
      961 agctccggcc cggttcgagg aagtcggccg agtagagcgc ccgtcccacc gcatcccacc
     1021 ggtcgctgcc aacggagtgc cagcgcaccg catccagcac gccgcggtcg accattccct
     1081 cgcgctcggc gcggttggcc gccgccgggc cgtgcaacag ctcgaccggc agcgcctcgc
     1141 tcccggccca gcggacgagc tcggcctccg gcgcatcccg cagcgcatcg tgaagccagg
     1201 ccgcccggcg ccagcgctgg cgttccgccg ccggcagccc cagcgcctcc gcccacccat
     1261 ccatgagcgc cgcgacccgc tcgatgtgcg cccgccgctc cgccgacacg gcggcccagg
     1321 gcgggagcag accggtctca gccacgccgg aggatcagtg ctcggcgcgc agcggcggcc
     1381 ggaccacccg attgtgtccg tcgacgaaga cgatccgcgg ctggaagcgc tccacctcgg
     1441 cctcgtcgac gaccccgtag gcgatgatga tgaccaggtc gcccgggagg cccaggcgcg
     1501 ccgccgcccc gttcagctgg atgacccccg agccgcgcgg gcccgggatg acgtaggtat
     1561 tgaaccgctg gccgttgttg acgttgacca cctgcacgtc ctcgaactcc accaggtcgg
     1621 cggcgtcgag caggtcctgg tcgaccgtca gcgagccctc atagtgcagg tccgccgacg
     1681 tgatggtggc acggtggatc ttggacttca tcagatgccg ttgcatcggt cccgactccc
     1741 tatcccagga tcacgttgtc gatgagtcgg gtggtcccca ccctggcggc gatcgcgacg
     1801 atcgtccccg ccccggcggt ccccgccggc gtgagcccgt cgggctcgac gacggcaata
     1861 taatcggcca ccacgcgctc cgaccccgcg agccgctcac cgacgatgac ctcgagccgc
     1921 ttgccggagc gctccccgcc ccgccatgcc gcgaccgccg cgtcgagcgc cgccgggagc
     1981 gcgagcgccg cccgccgctc gtcggcgctc aggtacgcgt tgcggctgga gagcgcgagc
     2041 ccatcggcat cgcgcacggt gggcaccatc acgagctcga cgggaaggtc gaggtcgcgc
     2101 accatcttcc ggatgagggt gaactgctgg atgtccttct gcccgaaaca ggcgaggtcg
     2161 ggctccacca ggtggaagag cttggccacg acggtcagca cgccggcaaa gtgcccgggg
     2221 cggacggcgc cctcccaccg ctcggccgcg gcacccggca cgacacgggt ctcggcgccc
     2281 ggcgggtaca tcgcctccac cgacggcacg aagaggaggt ccaccccccg ggccgacgcg
     2341 agggcgcggt cccgcgcgag gtcccgcggg tagcgggcga ggtcttcctt cgggccgaac
     2401 tggagcgggt tgacgaagat cgacatcacg accaggtcgg cccggcgccg ggcctcgtcg
     2461 accaggcgga ggtggccctc gtgcaaggcc cccatcgtcg gcacgaagcc gatccggcgg
     2521 cccgcgcggc gctcgtcgcc cagccagcgg cggagctcgg cgatggcggt gatctcgatc
     2581 atcgtgggat gcgggggggg cgggacgggg tcggcgcggt cactcgaagg agtggccggc
     2641 atcggggaac ttccccgcgc gcacttcggc cacgtactgc cggaccgcgt ccttcgtggc
     2701 gccggccagc gcgccgtact tcttgaggaa cttggggtcg aacccctcgt tgaggccgag
     2761 catgtcgtgg aggaccagca cctggccgtc gcagtggggg ccggcgccga tcccgatggt
     2821 ggggatgccg acggcggcgg tgaccttctt cgccaccttc gacggcacca gctcgagcac
     2881 caccgcgcac gcccccgcct cctcgaccgc gcgggcatcg gcgaggagcc gctccgcacc
     2941 ctcctggtcg cgcccctgga cccggtaccc gccgagctgg tggaccgact ggggggtgag
     3001 gcccaggtgc gccatcaccg ggatgccgac gtcggtgagc gcgtggatgg tcggcgccat
     3061 gtggcggccg ccttcgagct tgaccccgtg gacgttggcc tcctgcatcg cgcggccggc
     3121 gttccgcacc gcctcggcga cgctcacctg gtaggtgagg aacggcaggt cgatgaacac
     3181 gagcgtctcc cgggcgccgc ggcgcacggc ggcggcgtgg tagatcatcg cgtcgagggt
     3241 ggcgccgagg gtggtctcgt gcccgccgag gacctggtgg agcgagtcgc ccacgaggag
     3301 gaggtcgacg ccgctcgcct cgaggagccg ggcaaagagc gcgtcgtagc aggtgagcat
     3361 cgcgagcttc cgcccatcct gcttccaggc ggccaggtcg gggatcgcgc ggggacgggt
     3421 cttctcagcc ggcatgggct tcgagctcct tcagttctcg ttgccaccag tcccgctctc
     3481 cgagcgccgg atgtggcaag gtgagccccg gtccccggag cgtgagatca tcgtagcgta
     3541 cgatgtcgag gtcgagggtg cggggccccc agcgggtgcc gcgcacgcgg cccgccgcgc
     3601 gctcgatggc cagcagcgag tggaggagcg cgcgcggcgc gagctcggtc tcgagcagca
     3661 ccatctggtt gaggtacggc ccctgcggaa tgtcgccgag cggggcggtc tcctcaaccc
     3721 gggattccgc cagcacgcgg gtgccgggga gcgcgccgat ggcggcgcgg gcctgcgcca
     3781 agtgggcgtc ccggtcgccc aggttgctgc cgagggccac ccaggcccgc gccggcctcg
     3841 tcacgcgcca gccgcgaagt ggcgggcgag cgcctgcagg taggtcgcca ccccctcgag
     3901 ctcgcggatg gcgcgctcca ggtcgatctg gccctgcgcc gcctcctgcg cctggctggc
     3961 cagcgagttg gtctcctcgc gcgtgcggta cgacaccagc gccaggctct cgatctggct
     4021 gcggagtttc ttgaacgcct tggactggcc cgcggcggca tcggcgatgc ggcgggcgtg
     4081 gccgccggcg gcgtgggtgg acccgacgat cgcctcgagg gcctgcaccg cgtcgccgga
     4141 gagctcctcg accccctgca ccaccgcctc gccccggcgc atctgctccg agatctccga
     4201 gacctgggcc gtgatgcgcg ccaccagcgc gcccgcgtcg tgggacgcgc gggtgctctg
     4261 ctccgcgagc tgccggacct cgcccgccac caccgcgaac cccttgccgt gcttgccggc
     4321 gcgcgccgcc tcgatggccg cgttgagggc gatcaggtcg gtcatgtcgg cgatgtcgcg
     4381 gatggagccg atgaagccgg tgatcttccg ggtcaccgcc ccgagcgccg ccacctgctc
     4441 ggcgctctcg gcgacgaacc ccttgacctg caccaggcgc tggagcgcgt cggcgatggc
     4501 gtcgcggttc cgcgccgccg tttcgctcgc cgactcgctc gcgtcggcgg cggtggcgcc
     4561 gtcgcgcgcg gcctcctgcg cggccttggc cagctgctcg gtggcctcga gcccgccgac
     4621 cacgaaggcg tcctgctccc cgagttcggt gcggatggcc tgcgccgccg aggccaccgc
     4681 cgcggcggac tcccggaccg cgtcggtggc ccgcgccagc gcgcgcacct gcacgccgat
     4741 ccgctcgacg gtctccatca gcgggcgccg gagctccgcc aggtggatcg cggtggcgag
     4801 ctggcccgcg aaggccagca ccgtcgccga ttccttggcg ccataggagt gccgcttgtg
     4861 gtgctcgagc tccagggtgc cgaccgtgtc ctccccgaag cggagcggca cgatgagcac
     4921 gctcgccgcg tggggggagg gttcccggat ccgccggtcg cgggtcgcgt cggtcaccaa
     4981 tactggcttc cccgtctcga tggcctcggt tcgcagcgtg gccgagtcgg ccggcggctc
     5041 gccccgcccc ggccggccgc gcaccccccg gtaggtgagc accgccgaac cctggtcgac
     5101 gcggtagatg cggaagtcgc tccagtcgag gagccggtgc gccagggcct cgatctgggt
     5161 gcaagactcc tgcaggctca cgttgctggt gacgatcttc tcccgcgcgt ggaccttgct
     5221 gagctcctcc gccccgatgg cctcctcgag gatcttcttg gtgagcaccc cgagcaccgc
     5281 gagcacggca atgaccgtga cccagccgat cggcgccagg gtctccaccg cgccaacgag
     5341 gatgacggcg gccgcgaccg tgagcaggta cgccacgacc tcataccgca ggatcagcag
     5401 ccgctcgtcg agctcaagct tgtcgcgcag gaggagcgtg ccgtagaaga gcgcgcggcc
     5461 cgtgaagaag tacccgatga cgagcgtgat ggcggccggg aggtagtcga gcgagagccg
     5521 ttcggcgccg gtcatgtgcc gcacgaaggc gtgcatgccg aacgccgcca cgaaggccgc
     5581 gagctcgcgc ccggcgttga tccagctgaa gagccagtgc ttgcggtgga gtgcccagtc
     5641 ggccaggaac accccggtca cgaggccgat ggagaccgcc cccggcccca gcaggaccgc
     5701 cccgacgagc accggcacgc cggtctgcgt caggtacgag tacttgctga gctggatcgg
     5761 cgaggcgcgg agtgcgagca cggcgaaccc gagcgcgacc gcaccccagg ggtgcgccag
     5821 ccacgtccgg tcgaccagca gcactgccgg aaagagcacg acgcccacga ccgcaagcga
     5881 gcgggtgaat ccgcggaccg ccgaatcgac cgggatcctc acgccgcctc cccgtcgatg
     5941 gccccacgca gctccccgag cagccgctcg agccccgcga cgccctgctg tcccagcgcc
     6001 tcgaccagcg cgctgccgac caccacgccg tccgcggcgc gcgccaccac gcccgcctgc
     6061 cgggcggtgg ccacgccgaa cccgaccgcc accggcaacg acgtctgcgc ccgcacccgc
     6121 ccgacgctcg cggccaggtc cgccgcgggc gcggcgctcg tgccggtcac tcccaggcgg
     6181 ccgatcaggt agatgaatcc gcgggccccc gcgaccgcct ccccgatgcg cgccacgctc
     6241 gacgtcggcg ccaccagccg gatgaggtcg agcgacgaac ccgccaccgc cccttccacg
     6301 gcagcgtcgc cgccggcggg aaggtcggtg aggaggagcc ccgaaacgcc cgcggccgcc
     6361 gcatcggcca ggagccgatc cacgccgtac cggagcaccg gattgaggta gctgaagagg
     6421 accaccgggc ggcgggcatc gagtcgggcc acgaggtcca gggtgccggc caggctcatc
     6481 cccccggcca gcgcctcgaa gctcgaacgc tggatgatgg ggccgtcggc cagcgggtcg
     6541 ctgaagggca ccccgacctc gacgatgtca ccgaccgccg ccgcgagccg gagggcctcc
     6601 aggctggtgg cccggtcggg gtagccggcg gtgaggtagg ggatgaacgc ggtgcgatgc
     6661 cggcgcctga gctggtccca ggcgccggag agcggggtcg catcagacaa agtagtcgcc
     6721 gacagtgagt ccgtaccggt cgggggaggt caccttgacg atggaccggc ggtagtggcc
     6781 ggcctcgtcg agatcgccga ggtccacctg ctgacccggc ggcggataga taccgccatc
     6841 ggcatcgagc gccaaccgca ccagcggccg gttgaggagc gtcgggtcag ggttggcccc
     6901 ggccaccacc ccgacctcca gggtgtcgag cacaaccacc gtcccgaccg gatagacacc
     6961 gaggaggttg atcagcgcct tgaccatcac gcggtcgtag ccccggcggg ggttgtccca
     7021 catctccttg agcacctggt ccggctggat cggcacggtc tggtagctcc ggcggctggt
     7081 ggccgcgtcg aacccgtcgg ccaccgccac caaacgggag taggtgccct gctcccggga
     7141 gcggatgacg cgcgggtacc cgctcaggtc ggtcttcatg tggtgctcgg ccgccacgat
     7201 ggccgcgcgg tacgggatct cgccgtaccc ccgcagcccg aagagcgtga gcacgccgag
     7261 ccacggatgg gactgcatca cgcgccactc gtcctcggtc aggccgccgg tcttgttgag
     7321 gatctcgagc ggcacccgcg ccttccccac gtcatggagc agcgccgcga ggcccaggtc
     7381 gtacagctgc tgtttggaga ggcccaggcg cttcccgatg gccacggaaa agatgcagac
     7441 gttgaccgag tgggtgaagg tgtattcgtc gtagtcgcgg atcgtggtga gcccgaccag
     7501 cgaggtctcg ttgttgagca cctggtcgac cacggcctgc accgcccgct tcatcttctt
     7561 gacgctcggc gcccggccca gccgcgcccc ggtcatgacc tcgcgggtga cggccacccc
     7621 ctgcgagtac gtccgcttgg ccgcataccg ctgcgactcc gcgtcgccgg ccgcctcggc
     7681 ggaatcgctg gtggtctcga ggtggatgtg ctcgacctgg gcgccggcca tccggccggt
     7741 gagttcgctg aacttggtcc ctcccgccgc cgcgggccgg ccggtgaggg tcaggagcag
     7801 gctcaggaac gcctgccact cgcgccgctc gatcccctcg tcgatccgga ccgcgccgac
     7861 gtcgaactcc cggaagatgc cgaggatgga gctgaacgag gcgtagttgt cgagctcgag
     7921 ccggagccgg gtggagttga cgaaaatgaa gtcgccggcc acgcggacct cgatctcgtg
     7981 ctcggtctcg agcagtgccg ccgtcgcggc ctggaggtcg tcgagcgacc gctgcacggt
     8041 cgcgttctcg accgggtaga gcttgagcga ccggtgggcc gaatagaaca ggagcaggag
     8101 cccgcgtccg ccgtggcgga gggccgcctc gccgctcccg gtgcccgcgg agctcaccgc
     8161 caccgacgcg cgctgcggcg tcggcgtggt gcgccggggc ggggtcgcgt cgtcgctcac
     8221 gcgggcacct cgcggagggc gcggttggcg gcgttgcgca cgacggcctc cttgtcctgg
     8281 gtggccttct ggacgatggc ccgcacgtcg gggtgcctga tcttggcgat cgcggccacc
     8341 gcgcaggcgc gggtgtcggg gctcgccttc cgcttgaaga gcccgcccga gggttgcagc
     8401 agttcggtga gcggcgcgag cgcggcggcc ccggcgatga gcgcgaaggc ctcgaagaag
     8461 gcctggcgct cggtcaggtc ccggtcctcc gccgtcttgg ccagcaccac cgactcgacg
     8521 gtcttgaggg ccccgcggtg cgcccgcccg ccgagcacgc ggaccgtata gaggcgcacc
     8581 tcccggtcgg cgtcgtcgag cgcgcgctcg agcgcggcga aggccccggg cgagccgatc
     8641 tcgcccagcg cctgcgcgga ggcgagccgc acctgcgggt cggcgtggcc caggttctgg
     8701 cccagccccg gcaccgaggg ctggagccgg agccgcccgg agagcgtgat ggccgagccg
     8761 agcgcctcgc tctcgggcga ccccaggatc cggaggatct cggccgggtt ggcctccgca
     8821 agctgcaccg ccacccgctc gagcatctcg cgggcctgcg cgttggccgc cttgggcagc
     8881 cacaccatga ccggctcgag cgcgctgccg cggagttcgc ggaagagctc ggtcaggtcg
     8941 gcctccgccg gaggcgcgtt ggcctcgtcg atggcccgga ggagttccgc caccacgttg
     9001 ggctcgctca gctggcgcgc gaacatctcc aggcgcgccc ggtgcttctc gtcgagcgcc
     9061 cggccgcgga gggccagcac cttcgtttcc cggagcatct gcgccaccga acgcaggtcg
     9121 ccggccgcca ggaggtaagc cgggaagctc tccaggatca tcaggatctc gtcgcggacc
     9181 tcggccgagg tctggatctc gaagaggtcg tagaggaacg ccatcacgtt ggcgcggagg
     9241 tcctggtcgt attcctttgc gatctcccgc gcgacgtagg cgatctcctg ctcgtcgagg
     9301 aagtacaggg tcgagtcgaa gtcctcgagg cgcaccaccc ccgccggccg cggcgtggcg
     9361 gcatcctcgg tgaccgcatc gcggttgcgg gcccgggcgt cctcggccgt gacgggcgcg
     9421 tcggtcccgc ccaccacgaa ccccgccacc ggcgggccgg ccaccgcggg cggcgcggcg
     9481 tcggtcagca cttcgctgaa cgtgtaatgg atgagctgga aatcctgctc ccagagcagc
     9541 gtcaggaggt catcctcggc ctcgga
//
LOCUS       DASUAP010000145         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_590035, whole genome shotgun sequence.
ACCESSION   DASUAP010000145 DASUAP010000000
VERSION     DASUAP010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590035"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VFU45_03490"
     CDS             <1..1457
                     /locus_tag="VFU45_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358161.1"
                     /translation="YQGRGAASVRYVIDGVPWLPMGPDSVAVDPAHFPLTLLQRVEIE
                     RWPGQLVVRLYTRRDDRLAPRSRIVVAAGDRKFARFGGGLERHYASGIGFALGADYLS
                     APTRSGENTDASDLAILLRGSYVPTPRLGFEYQLIRTSPEHGTWQLFANTDSIGRSLK
                     GDRTDGYFRGFFRTGTEGNGAEFDVLAGSSSWNGDSVSQSAGLVGATASYRQPTWRVG
                     ASAMSWSRWTPLDSRMDGGWTPLAGLAVSGEAVHQRHDGGRESNWVGLQAGITLPESL
                     VLNGSVRAGSEVGAPSLLADPAQDLRDWRVAAGWQRRVVGAEAAWVHTAAYRPLGFQP
                     YLQVPGLAPVGAANWLEVSARVSPRQWLLLEARYGDPQRVSVDGVPPTHSIITATIRS
                     KFLRQYRSGIFDLKLQLAMESWGRGIIGRDSTGAAISLPGATFFRGQLELRLGSLVFY
                     YDRMNLRSTLLGYVPGFEIPPGGQTFGVRWDFVN"
     gene            1536..2402
                     /locus_tag="VFU45_03495"
     CDS             1536..2402
                     /locus_tag="VFU45_03495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEU5358162.1"
                     /translation="MTGYGAAEAAVAGGRLRVEVRTVNHRYFNLALRWPDDLALLEAD
                     LRETLRKEFDRGHVAVQGRWVERTERHAPLALDLDRARQAAERLRELQAALTLPGQVT
                     LELVARQPDVLGAAAPPAEAAVAWADVAPIVGQAVAECRAMRRKEGAVLAGELEHRLA
                     LMTERCDAVAGLAPGRLERERDRMRGTVAQLLDGRQVDEARLAQELAFLADKLDITEE
                     LVRFRAHVAACREALQGSDAVGKRLGFLAQELGREVNTMGSKANDAAITLHVVAMKGE
                     LEKFREQLENLE"
     gene            2399..>2651
                     /locus_tag="VFU45_03500"
     CDS             2399..>2651
                     /locus_tag="VFU45_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU5358163.1"
                     /translation="MRPFLLVLSAPSGCGKTTIARRLLEQRPGLAYSVSATTRPPRAG
                     ERDGQSYHFLSPEAFERRVRAGDFLEWAEYAGHRYGTLRS"
BASE COUNT          361 a          884 c         1027 g          379 t
ORIGIN      
        1 attatcaggg acggggcgcc gcgtcggtgc gctacgtcat cgacggggtg ccgtggctcc
       61 cgatggggcc cgacagcgtc gcggtggacc cggcccactt tccgctcacg ctcctgcagc
      121 gcgtggagat cgaacggtgg ccgggccagc tggtggtgcg cctctacacc cggcgggatg
      181 accggctcgc gccgcgttcg cggatcgtcg tcgcggccgg cgaccggaag ttcgcccggt
      241 tcggcggggg gctcgagcgc cattacgcgt ccgggatcgg cttcgcgctg ggcgccgatt
      301 acctgagcgc gccgacccgc agcggcgaga acaccgacgc ctccgacctg gccatcctcc
      361 tgcgcggaag ctacgtgccg acgccgcggc tcgggttcga gtatcagctc atccgcacgt
      421 ctcccgagca tggcacgtgg cagttgttcg ccaacaccga ttccatcggc cggagcctca
      481 agggcgaccg caccgacggc tacttccgcg ggttcttccg caccgggacg gaggggaacg
      541 gggcggaatt cgacgtgctg gcgggatcga gcagctggaa tggcgacagc gtaagccaga
      601 gcgccgggct ggtgggcgcg accgcgtcgt accgccaacc cacctggcgg gtgggcgcga
      661 gcgcgatgtc gtggagccga tggacgccgc tcgacagccg gatggatggc ggctggacgc
      721 cgctcgccgg cctggcggtg agcggcgagg cggtgcatca gcggcacgac ggcggacggg
      781 agagcaactg ggtcggcctg caggcgggga tcaccctgcc ggagtccctg gtgctcaatg
      841 gaagcgtgcg cgccgggtcg gaggtcggcg cgccttcgct cctggcggat ccggcgcagg
      901 acctccggga ctggagggtc gcggccggat ggcagcgccg ggtcgtgggc gctgaggcgg
      961 cgtgggtgca cacggcggcc taccggccgc tcgggttcca gccgtatctc caggtcccgg
     1021 ggctggcccc ggtcggcgcc gccaactggc tggaagtcag cgcgcgggtg tcgccccggc
     1081 agtggctcct cctcgaagcg cgctacggtg acccgcagcg cgtctcggtc gacggtgtcc
     1141 cgccgaccca ttcgatcatc accgccacga tccggtcgaa gttcctccgc cagtaccgga
     1201 gcgggatctt cgacctcaag ctccagctgg ccatggagtc gtgggggcgg gggatcatcg
     1261 gccgggactc gaccggggcc gccatatcgc tcccgggcgc cacgttcttc cgggggcagc
     1321 tcgagctccg gctcggaagc ctcgtcttct attacgaccg gatgaacctg cgaagcacgc
     1381 tgcttggcta cgtgccaggt ttcgagatcc cccccggggg gcagacgttc ggggtgcggt
     1441 gggatttcgt gaactgagcc gcactgccgc caagtccaag accagcctat atttcacgcc
     1501 ttttccgcca ccgtgagggc gcgctgccgt acagcatgac ggggtatggg gccgcagagg
     1561 cggcggtggc cggcgggagg ttgcgggtcg aggtccgcac ggtcaaccac cgttatttca
     1621 acctggccct ccgctggccc gatgacctgg ccctgctcga ggccgacctg cgggagacgc
     1681 tccggaagga gttcgaccgg ggccacgtgg cggtgcaggg ccggtgggtg gagcggaccg
     1741 agcggcatgc ccccctggcg ctcgacctcg accgggcccg gcaggccgcc gagcgcctgc
     1801 gggagttgca ggcggcgctg acgctccccg gccaggtcac cctcgagttg gtggcccgcc
     1861 agcccgacgt gctgggggcg gcggcgccgc ccgccgaggc ggcggtcgca tgggccgacg
     1921 tcgcgccgat cgtggggcag gccgtggccg agtgccgggc catgcggcgc aaggaaggcg
     1981 cggtgctcgc tggtgagctg gagcaccgcc tggcgctgat gaccgagcgc tgcgacgccg
     2041 tggccgggtt ggcgccgggc cggctcgagc gggaacggga ccggatgcgg gggacggtgg
     2101 cgcaactgct cgacggccgg caggtggacg aggcgcgact ggcgcaggaa ctggcgttcc
     2161 tggccgacaa gctcgacatc accgaggaac tggtccggtt ccgggcccac gtcgccgcct
     2221 gccgggaggc actgcagggg agtgacgcgg tcgggaagcg gctgggattc ctggcccagg
     2281 agctggggcg cgaagttaac acgatgggct cgaaggccaa cgacgcggcc atcaccctcc
     2341 acgtcgtcgc gatgaagggc gagctcgaga agttccgcga gcagctggag aacctcgagt
     2401 gaggcccttc ctgctcgtgc tgtcggcgcc gtcgggatgc ggcaagacga ccatcgcgcg
     2461 gcggttgctg gagcagcgcc ccgggctggc gtattcggtc tcggcgacca cccggccgcc
     2521 gcgcgccggg gagcgggacg ggcagtcgta ccacttcctg tcaccggagg cgttcgagcg
     2581 gcgggtccgc gccggggact tcctcgagtg ggcggagtac gcggggcacc ggtacggcac
     2641 gctgcggtcg g
//
LOCUS       DASUAP010000146         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_780711, whole genome shotgun sequence.
ACCESSION   DASUAP010000146 DASUAP010000000
VERSION     DASUAP010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780711"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1793)
                     /locus_tag="VFU45_03505"
     CDS             complement(<1..1793)
                     /locus_tag="VFU45_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing serine/threonine
                     protein kinase"
                     /protein_id="HEU5358164.1"
                     /translation="MPLERLNAALADRYLIERELGAGGMATVYLAHDVRHDRKVAVKV
                     LRPELAAVIGAERFLSEIKTTANLQHPHILPLFDSGVASVQGHESRVTSHAESADPEG
                     GATRDPRPASYLFYVMPYVEGESLRDRLNREKQLPIPDAVAIAIEVAGALDYAHRHGV
                     IHRDIKPENILLHDGRALVADFGIALAAHKAGSTRMTETGMSLGTPHYMSPEQAMGER
                     EITPRSDVYALGCVLYEMLCGEPPFSGPTAQAIVAKVLTEEPRPLLPKRHTIPPHVEI
                     AVLTALEKLPADRYATAAEFVTALQRPDVDTPSTLPGGVRSTRANRRRWRLWAPWGVA
                     LAAAAVTAWALLGRGGPPQVTWNYVTLSDSIPITTRGPSLAISPDGQSIVYKDDHQNG
                     TLWIKRADQLDPQPIPGTVRGFSPVFSPDGKWLAFTADGQVRKIRLGGGATVTLADSA
                     ATQIYGLAWLDDGSIIYTAARNDQLLRVSANGGASTVVMADPALRGVGVLNPNPLPHA
                     RGVLFLACASGCVTASIHVLDLKSGTQKPLIDDAIDAWYLPTGQLLYVRRDGAALVAP
                     FDLDKLEITGAAVPVLDGVQINISITMLAWSP"
     gene            complement(1797..>3012)
                     /locus_tag="VFU45_03510"
     CDS             complement(1797..>3012)
                     /locus_tag="VFU45_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358165.1"
                     /translation="VDLRTGIRHVLVKGLLARYVAGYLVYLRADGAVLAAPFDQDALR
                     ITGPSVTLFDGVGVKALGSADFAIAPEGTLAYVRGANGGNEGSIEFVTLDRSGKVTPL
                     TPRTTFTPGTHPTLNISPDGRAVAFDMLQGATDNVWIRSLTTGALTRLTFDAHGAAEG
                     TWSPDGRWVIYTSLDDSGTSDIWRRRADGSAPPERLLRLPGTRINYAMMSPDGRWLVY
                     TALGNVDKTEDIFARRLTGDTTTVPLVTGTFLRGLPTFAPDGRWFAYASVESGQPEVY
                     VRPFPRAGDGRWQVSLAGGISPRWAQDGRQLYFIDAANDLVSVPVHAGTTFGFDPPTT
                     AIAAHAAGNLTIGITGVQRYDILPDGRFLTMRLAAAGDDRLDDPLVVVDNWVSELKAR
                     MRATNASAAEGN"
BASE COUNT          420 a          995 c         1145 g          452 t
ORIGIN      
        1 ggcgaccagg ccagcatcgt gatgctgata ttgatctgga ccccgtcgag gaccggcacc
       61 gcggcgccgg tgatctcgag cttgtcgagg tcgaacggcg ctacgagcgc cgcgccgtcg
      121 cgccggacat agaggagctg cccggtgggg aggtaccagg cgtcgatcgc gtcgtcgatg
      181 agcggcttct gggtgcctga cttgaggtcg aggacgtgga tcgaggcggt gacgcacccc
      241 gacgcacacg ccaggaagag cacaccgcgg gcgtgcggga gcgggttcgg gttgaggacg
      301 ccgacgccgc ggagcgcggg gtcggccatc acgacggtgc tggcaccgcc gttcgcgctc
      361 acccggagga gctggtcgtt tcgcgccgcg gtgtagatga tgctgccgtc gtcgagccac
      421 gcgagcccgt agatctgggt ggcggcggag tcggccagcg tcacggtggc gccgccgccc
      481 aggcggatct tccggacctg gccgtcggcg gtgaaggcga gccacttccc gtcgggggag
      541 aagaccgggc tgaacccgcg caccgtgccg gggatcggct gcgggtcgag ctggtcggcc
      601 cgcttgatcc agagcgtccc gttctggtgg tcgtccttgt agacgatgct ctgcccgtcg
      661 ggggagatgg ccagggacgg cccgcgcgtg gtgatcggga tgctgtcgct cagcgtcacg
      721 tagttccacg tgacctgcgg cgggcccccg cgccccagga gcgcccacgc ggtcacggcg
      781 gcggccgcga gcgccacacc ccacggcgcc cagagccgcc accgccgccg gttggcgcgc
      841 gtgctccgga cgcccccggg aagggtggac ggcgtgtcga cgtccggccg ctgcagcgcg
      901 gtgacgaact ccgccgcggt ggcgtagcgg tcggcgggga gtttttcgag cgcggtgagg
      961 acggcgatct cgacgtgcgg cgggatcgta tgccgcttgg ggaggagcgg gcgcggctcc
     1021 tcggtgagca ccttggccac gatggcctgt gcggtggggc cgctgaaggg gggctcgccg
     1081 cagagcatct catagagcac gcagcccagc gcgtacacat ccgaccgggg cgtgatctcc
     1141 cgctctccca tcgcctgctc ggggctcatg tagtgcgggg tgccgaggga catcccggtc
     1201 tcggtcatcc gggtgctgcc ggccttgtgg gccgcgagtg cgatgccgaa atcggccacg
     1261 agggcgcggc cgtcgtggag gaggatgttc tccggcttga tgtcgcggtg gatgacgccg
     1321 tggcgatggg cgtagtcgag cgcccccgcc acctcgatcg cgatggcgac ggcatcgggg
     1381 atggggagct gcttttcccg gttgagccgg tcgcggaggg actctccctc gacgtagggc
     1441 atgacataga acaaatacga cgcgggtcgc gggtcgcggg tcgcgccgcc ctcgggatcc
     1501 gcgctctccg cgtgactcgt gacccgtgac tcgtgaccct gtactgacgc gacccccgaa
     1561 tcgaacagcg gcaggatgtg cgggtgctgc aggttggcgg tggtcttgat ctccgacagg
     1621 aagcgctcgg cgccgatgac cgcggcgagt tccggccgca ggaccttgac cgcgaccttg
     1681 cggtcgtgcc ggacgtcgtg ggccaggtac acggtggcca tcccgcccgc gccgagctcg
     1741 cgctcgatca ggtagcggtc ggcgagggcg gcgttcagcc gttcgagggg caccgatcag
     1801 ttcccttccg ccgcggaggc gttcgtcgcg cgcatcctgg ccttgagctc gctcacccag
     1861 ttgtccacca cgaccagcgg gtcgtccaga cggtcgtccc cggcggccgc gaggcgcatc
     1921 gtgaggaacc tgccatcggg aaggatgtcg tagcgctgca ccccggtgat gccgatggtc
     1981 aggttgcccg ccgcgtgagc cgcgatggcc gtcgtcggcg ggtcgaagcc gaaggtggtg
     2041 ccggcgtgga ccggcacgct caccaggtcg ttggcggcat caatgaagta gagttgccgg
     2101 ccgtcctgcg cccagcgcgg cgagatcccg cccgccaggg acacctgcca ccggccatcg
     2161 ccggcccgcg gaaaggggcg cacgtacacc tccggctgcc ccgactccac cgacgcatac
     2221 gcgaaccagc ggccgtccgg agcgaacgtg ggcaagcccc tgagaaaggt acccgtgacg
     2281 agcgggacgg tcgtcgtgtc gccggtgagc cggcgggcga agatgtcctc cgtcttgtcg
     2341 acgtttccga gggcggtgta gaccagccac cggccgtcgg gcgacatcat cgcgtagttg
     2401 atgcgcgtcc ccgggaggcg cagcagccgc tccggcggcg cgctcccgtc cgctcggcgg
     2461 cgccagatgt cggaggtgcc gctgtcgtcc agcgacgtgt agatgaccca ccgcccgtcg
     2521 ggggaccacg tcccttccgc ggcgccgtgg gcgtcgaagg tgagccgggt gagcgcgccc
     2581 gtcgtgagcg accggatcca cacgttgtcg gtggcgccct gcagcatgtc gaacgccacg
     2641 gcccggccat caggcgagat gttcagcgtg ggatgggtgc ccggagtgaa cgtcgtgcgc
     2701 ggggtgagcg gcgtgacctt gccgctgcga tccagcgtga cgaactcgat ggacccttca
     2761 ttgccgccgt tcgcgccgcg gacgtaggcc agcgtgcctt cgggagcgat cgcgaagtcg
     2821 gcggagccca gcgccttgac gccgacgccg tcgaacagcg tcaccgacgg cccggtgatc
     2881 ctgagggcgt cctggtcgaa cggggcggcg aggaccgcgc cgtcggcgcg caggtacacg
     2941 agatatcccg cgacgtagcg cgccaggagc cccttgacga ggacgtggcg gatcccggtg
     3001 cggaggtcga cg
//
LOCUS       DASUAP010000147        11134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1176284, whole genome shotgun sequence.
ACCESSION   DASUAP010000147 DASUAP010000000
VERSION     DASUAP010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11134
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176284"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VFU45_03515"
     CDS             <1..1071
                     /locus_tag="VFU45_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5358166.1"
                     /translation="VSDPEANHFHKQTLNAVAQLRQRQSGKLSGTLGLWTDIEGLTIG
                     GDQPLGPNSTTTGVAGYAFEEYAAAPGTRLQAGVRYDYNRIQTHPDPTSADSTFRTLD
                     TSRLSNAVTASLGLVHQLSRDLTLSVNVARSFRAPTVQELFAQGLDAASGTYTVGTAG
                     LAPEHGNGIDASLKGSSGRVAFELSPYWNSIDDYIFGFLRGDTIQGLPVRQFSATSAR
                     LWGFEAAVTVEPAAHLALRASSDYVNAEDTRLHQPLPFTPPLRGLLRASWQDDRYSGM
                     VEGRFAASQTRLGNGDTPTDGYAVMNLGVGIRLIEARMVHSVTVHVDNVFNTVYRDNL
                     SVIKDFIPQPGRGVRVGYEVVF"
     gene            1520..1792
                     /locus_tag="VFU45_03520"
     CDS             1520..1792
                     /locus_tag="VFU45_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5358167.1"
                     /translation="MGQRLRAWRLARDLTVQELAARAALTPLTVLKAERGENFTFRTL
                     LRILRVLGALEQVDLILPPPPPSPLALLDRRPPPPPRRRARRKPRG"
     gene            1785..3059
                     /locus_tag="VFU45_03525"
     CDS             1785..3059
                     /locus_tag="VFU45_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEU5358168.1"
                     /translation="MAEFTVFAEVRLWGHRVGAVAEDDAGGVTFEYAPEFRRLGLEIS
                     PRHLPLARAGPLSFPALRRVDAFQGLPGVLADALPDRFGNAVIAQYFARAGRAQDALS
                     PVQRLLYIGRRAMGALEFEPAIDRPRTPAESEALEVKGLVDEARRIVAGDVDVALPEM
                     MQLGASAGGAHPKAVILWDPARKSVRSGFAEPAAGEEAWIIKFDGVGELHHPDPAPEP
                     YNRIEAAYVAMARAAGLETADVRLLEERRLAHLLSRRFDRAGSERLHLHSLGGLDHAD
                     FNAPGTYSYEQYLRVVLELGLPPAALEEAYRRAVFNVVAVNQDDHVKNFAFLMDRAGT
                     WRLAPAYDLTFARGSGFTRHHQLALNGKVDDFRRADLLALGARFGVRRDGAAVIDQVV
                     DAVGDWPRYAAATGVPKARVTAIGAAHRRMGA"
     gene            complement(3323..3634)
                     /locus_tag="VFU45_03530"
     CDS             complement(3323..3634)
                     /locus_tag="VFU45_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358169.1"
                     /translation="MSTYKPASSATIRALVIGALLSFSAVAALSAQQAAIQSPVAAAP
                     AVPAPHASALFARGDADVLTPATAEAAPARANGEGRNHTIVVSTLVLVLSVVILVLLI
                     K"
     gene            complement(3693..4754)
                     /locus_tag="VFU45_03535"
     CDS             complement(3693..4754)
                     /locus_tag="VFU45_03535"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEU5358170.1"
                     /translation="MLGALVALGLAAGACNNSTNPTNPLTTVPTVTSTGPGSASVGVA
                     RNASITATFSEAMDTATINATTFTLHQGLTAIPGVVTYAGNTATLNPTNTLDPNTSFS
                     ASISTGARDLQGDSLAAMKTWTFTTVASSPSGPAAVNLGTAGNYVILSKSGISTTGTT
                     AITGDIALSPAAASFITGFALTTPTTFSTSSLVTGHVFASNYAVPTPATLTTAVLDMQ
                     TAYTNAAGRTLPDFVNLGAGNVQGLNLVPGLYKWGTGVSIPSAVTLTGGATDVWIFQV
                     SGNLTVGNGAIVTLAGGAQASNIFWQVTGSTTIGTTAQMKGVILSKTLISMNTGSSLT
                     GRDLAQTAVTLNASTVTHP"
     gene            complement(5001..5696)
                     /locus_tag="VFU45_03540"
     CDS             complement(5001..5696)
                     /locus_tag="VFU45_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5358171.1"
                     /translation="MKSTSRRPGASRARKPPLRLITVAVIEDNRLVREALSAMLGRLP
                     DLRVISLDTADPTFLAESKPDVLLLDVGLRDQDSLRVAAGLLEEHPEAFIIMMDVLPV
                     NEEIKEYVNIGVSGFVLKDATFDEFVATIRSVAAGVKVLPPRMTESLFAQIAREVEAL
                     EPTRAVEAVRMTPREREVIDLIGEGSSNKEIASKLNIATHTVKSHVRNVMEKLALHTR
                     LQIAAYSHREGEA"
     gene            complement(5771..6727)
                     /locus_tag="VFU45_03545"
     CDS             complement(5771..6727)
                     /locus_tag="VFU45_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358172.1"
                     /translation="MSPREQYEVLFTRHAANPILTAADWPYPAHTVFNPGAVRLKDGT
                     TLLLCRVEDRRGHSHLTAARSANGIDGWVIDRTPTFQPDAERYPEEFWGVEDPRITYV
                     PELGKYAVAYTAFSRGGPGVALALTEDFRTFERYGLVMQPDDKDAALLPRRVNGNFAL
                     IHRPMHDSGAHVWISYSPDLRNWGGHKLVLQARRGAWWDANKVGLSPPLIETPAGWLT
                     IYHGVRHTASGSLYRLGLALLDLEDPERCLRRGDMWVFGPEAPYERAGDVGNVAFPCG
                     CTIGDDGDTVNLYYGAADTSIALATGSVKQMLRWLEEHGGEA"
     gene            complement(6724..9042)
                     /locus_tag="VFU45_03550"
     CDS             complement(6724..9042)
                     /locus_tag="VFU45_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5358173.1"
                     /translation="MALARPLAPSSTPHAWSTTRSVEQQSRTPALRQVALLGNHLPRK
                     CGIATFTTDLGAAISDEFPALDCLVVAMNDAGPRHAYPPMVRFEIEENDVASYRRAVD
                     YLNVSTVDAVSLQHEYGIFGGKAGALILPLLAELRMPIVTTLHTILADPDPGQRRVMD
                     EICRLSERLVVMSRHSATLLTQVHGVDPGKIDVIPHGIPEVMFAEGDKDRLGVAGKSV
                     ILTFGLLSPDKGIEHVIDALPAILAEHPDTVYIVLGATHPHVLEGSGESYRLMLEARA
                     QRLGVDASIIFHNRFVSQAELGEFLSAADIYVTPYLNPEQSTSGTLAYAVGAGKAVIS
                     TPYRYATELLAEGRGILVPWRDAGAIAREVTGLLDDPRRTAAMRHRAAEHGRGMAWGA
                     VARRHVGSLERAVLAHAERRRTSFHARTLAERPASLPELDLDHLERLTDDTGILQHAT
                     FVVPRYAEGYCLDDNARALMLMVLLEESGTVDRKTLRKLASRYLAFVSHAFHPATGRF
                     RNFLTFARTWGELCGSEDSHGRALWALGTVVGRAVDPGRRSLSSGLFHAALNAVDELS
                     SPRAWAYALLGIDEYLRAFQGDTGVQATRARVAERLFGLYTRTSAEDWPWFEDHMTYC
                     NARLPQALIVSGAAMGRPDMVAAGTRALEWLGTLQHTPDGYFAPVGSNGFYRRGAAVA
                     RFDQQPVEACGMVSACLSAHRVTGDARWKRYADSAFGWFLGQNHLQLPVYDSSTGGCR
                     DGLHADRMNENQGAESTLSFLQALWDMRAVRA"
     gene            complement(9415..9987)
                     /locus_tag="VFU45_03555"
     CDS             complement(9415..9987)
                     /locus_tag="VFU45_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5358174.1"
                     /translation="MSAARLSFAVLIASTLLAARPAQAQGSSSTNPAFFIEGLGGAAI
                     PTFDIANDIKTGAMFGATFGYRLDPDWTLMAEFDYGHHKDKATNSVTIKTLHYMAKLG
                     YSLTGEREHGWDAMVNLGAGAVTFDGFGAAGTKTYFAINAGAKITYNFNRSVGFVISP
                     QGDIAFSKKAEVNTTNAWVWPVSAGLRIKF"
     gene            10784..>11134
                     /locus_tag="VFU45_03560"
     CDS             10784..>11134
                     /locus_tag="VFU45_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358175.1"
                     /translation="MLLLTTNPWRRLLLPTRAGYPLPLKSRAPANNEIVLLARVRRTP
                     AGRVHVVHTALRAVAVRLKPLSPWRGRSRWIIRAAHWKLAQPFQLEGIQLTPSEYRSR
                     GALVPLDPTDVDEIY"
BASE COUNT         1629 a         3778 c         4031 g         1696 t
ORIGIN      
        1 gtgtccgacc cggaagcgaa ccacttccat aagcagacgt tgaacgcggt ggcccagctc
       61 cgccaacgcc agtccggcaa gctctccggc accctcggcc tctggaccga catcgagggg
      121 ctcacgatcg gcggcgacca gccgctgggc cccaactcca ccaccaccgg cgtggcgggg
      181 tacgccttcg aggaatacgc cgcggcaccc ggcacccggc tgcaggcagg agtccggtac
      241 gactacaacc ggatccagac ccaccccgac cccacatccg ccgactcgac cttccggacc
      301 ctcgacacct cccggctctc caacgcggtg acggcgtcgc tggggctggt gcaccagctc
      361 tcgcgcgacc tgacgctctc ggtcaacgtg gcccgctcct tccgggcgcc cacggtgcag
      421 gagctctttg cccaggggct cgacgccgcg agcggcacct atacggtagg cacggcgggc
      481 ctggccccgg agcacggcaa cggcatcgac gcctcgctca aggggagctc gggccgggtg
      541 gcgttcgagc tctcgccgta ctggaactcg atcgacgact acatcttcgg cttcctccgc
      601 ggcgacacga tccagggcct cccggtgcgg cagttctccg ccaccagcgc ccggctctgg
      661 ggcttcgagg ccgcggtgac ggtggagccg gcggcgcacc tggccctccg cgccagctcg
      721 gactacgtca acgccgagga cacccgcctc caccagccgc tgccgttcac gccgccgctg
      781 cgcgggctgc tgcgggccag ctggcaggac gaccgctatt ccgggatggt cgaaggacgc
      841 tttgccgcaa gccagacccg cctgggaaat ggcgacaccc ccaccgacgg ctacgcggtg
      901 atgaacctcg gtgtcggcat ccggctcatc gaggcgcgga tggtgcacag cgtcaccgtg
      961 cacgtcgaca acgtgttcaa caccgtctac cgcgacaacc tctccgtgat caaggacttc
     1021 atcccccagc cgggacgggg ggtgcgggtg gggtatgagg tggtgttcta ggggcgggtg
     1081 ggcgcgtgag gcgcgtgaac tgccgcatga agcgggtgga cggccgcgtg gggcgcaggg
     1141 gcgcaggggc gcagaggcgc aggggcgcag gggcgcaggg gacctggtcc cacaccggag
     1201 tcatcctgag ggagcggagc gaccgaagga tctctcggcc caagtctcac caccaactcc
     1261 tgcgttgccc aagcctcacc ccctacccac cggcgccggt gggtcgccga ccggccacca
     1321 gatccgtgga gccggtcccg cccttccgca ggctcagggg aggctctgct cgggaaggat
     1381 ccgctgcctc cgtgtgcagt ctcaatagca tatattataa catatattat agcttgtaat
     1441 agcaggtaat atatgttata gtatctccct gaggcccagc actatccccg acgccacgga
     1501 ccccgagatc ctggccctcg tgggccagcg gctccgcgcc tggcgcctcg cgcgggacct
     1561 cacggtccag gaactcgccg cccgcgcagc gctcacgccg ctcacggtcc tcaaggcgga
     1621 acggggagag aacttcacct tccgcaccct cctgcggatc ctccgcgtcc tcggcgccct
     1681 cgagcaggtc gacctgatcc tgccgccacc gccgccatcg ccgctggcac tgctcgaccg
     1741 ccgcccgccg ccccctccgc gccgccgggc acggcggaag ccccgtggct gagttcacgg
     1801 tcttcgccga ggtgcgcctg tggggtcacc gcgtgggagc cgtcgccgaa gatgacgccg
     1861 ggggcgtcac cttcgagtac gcgccggagt tccggcggct cggccttgag atctcccccc
     1921 gccacctccc gctcgcgcgc gcggggccgc tgtcgttccc ggctctccgg cgcgtcgacg
     1981 cattccaggg actcccgggg gtcctggccg acgcgctccc cgaccgcttc ggcaatgcgg
     2041 tcatcgccca gtactttgcc cgcgccgggc gcgcgcagga cgcgctgagc ccggtgcagc
     2101 gcctcctcta tatcgggcgc cgcgcgatgg gtgcgctgga gttcgagccg gcgatcgacc
     2161 ggccgcggac gccggccgag tcggaggcgc tcgaggtcaa ggggctggtc gatgaggccc
     2221 gtcgcatcgt ggccggggac gtcgatgtcg cgctgccgga gatgatgcag ctcggcgcct
     2281 ccgccggcgg cgcccacccc aaggcggtca tcctctggga tccggcgcgc aagagcgtcc
     2341 ggagcggatt tgccgagccg gcggcagggg aagaggcgtg gatcatcaag ttcgacggcg
     2401 tgggggaatt gcaccatccc gatccggcgc ccgagccgta caaccggatc gaagcggcgt
     2461 acgtcgcgat ggcgcgcgcc gcggggctgg agaccgccga cgtgcggctc ctggaggaac
     2521 ggcggctggc ccacctcctg tcgcggcggt tcgatcgggc cgggagcgag cgactccatc
     2581 tccacagcct cggcggactc gaccacgccg acttcaacgc gcccggcacc tacagttacg
     2641 agcagtatct ccgcgtggtg ctcgagctgg gcctcccgcc agcggcgctg gaagaggcgt
     2701 accggcgcgc ggtgttcaat gtggtggccg tcaaccagga cgaccacgtc aagaacttcg
     2761 ccttcctgat ggaccgcgcc ggtacctggc gcctggcgcc ggcctacgac ctcacgttcg
     2821 cacgggggag cggcttcacc cggcaccacc agcttgcgct caacggcaag gtcgatgact
     2881 tccgccgggc ggaccttctg gccctcggcg ccaggttcgg cgtccgccgt gacggcgctg
     2941 cggtgatcga tcaggtggtc gatgccgttg gcgactggcc ccgctatgcc gccgcgaccg
     3001 gggtgccgaa ggcgcgggtg acggcgatcg gtgcggcgca caggcggatg ggcgcgtgaa
     3061 cggccgcgtg gggcgcaggt gcggagagac gcaggggacc tcttcccaca gcggagtcat
     3121 cctgagggag cggagcgacc gaaggatctc tcggcccaag tctcaccacc atctcttgcg
     3181 ccgcccaacc ctcgccccct acccagcctg cacggatggg ttgctagtag accaccggat
     3241 ccggggacag gcgggatgac acttcgctcg tgcacgggac ccccgagcgc cgacactccg
     3301 cacgcgaggg cgcccgccgc actcacttga tcaggagcac caggatcacg acgctgagga
     3361 ccagcaccag cgtcgacacg acgatcgtgt ggttgcgccc ttcgccgttc gcgcgcgcgg
     3421 gggcggcttc cgcggtggcc ggggtcagga catccgcatc gccgcgagcg aagagggccg
     3481 aggcgtgcgg ggcggggacg gccggagcgg cggcgacggg ggactgaatt gccgcctgct
     3541 gggcggagag cgccgcgacg gcggagaagg agagcagggc gccgatgacc agcgcccgga
     3601 tggtggcgga cgaagcgggc ttgtatgtcg acattggtgg ctccaggacc cggtgacccg
     3661 caggatgtcc tgcgggttgt ggcgtggtcg gactacggat gcgttacggt cgatgcgttg
     3721 agcgtcacgg ccgtctgcgc gaggtcccgg cccgtgaggc tgctgccggt gttcatcgag
     3781 atgagggtct tcgagaggat gacacccttc atctgcgcgg tggtgccgat cgtggtggag
     3841 ccggtcacct gccagaagat attgctggcc tgggcgccgc cggcgagggt cacgatcgcc
     3901 ccgttgccga ccgtcaggtt cccggagacc tggaagatcc acacgtccgt ggccccgccc
     3961 gtgagcgtga cggccgacgg gatcgagacg ccggtgcccc acttatacag gcccgggacc
     4021 aggttgagcc cctgcacatt gcctgcgccg agattgacga agtccggcag ggtgcggccg
     4081 gccgcgttgg tgtaggcggt ctgcatgtcg aggaccgccg tcgtcagggt ggcgggggtc
     4141 ggcacggcgt agttggacgc gaagacgtgc ccggtcacga gggacgaggt ggagaaggtc
     4201 gtcggtgtgg tgagcgcgaa gccggtgatg aacgacgcgg ccgcggggct cagggcgata
     4261 tcgccggtga tggcggtggt gccggtggtc gagatgccgg acttgctgag gatgacgtag
     4321 ttgcccgccg tgccgaggtt gacggcggcc ggaccggacg gggacgatgc cacggtggtg
     4381 aaggtccagg tcttcatcgc cgccaggctg tcgccctgga ggtcgcgggc gccggtcgag
     4441 atgctggcgc tgaagctggt gttcgggtcg agcgtgttgg tcgggttcag ggtcgccgtg
     4501 ttgccggcgt aggtcacgac gccggggatg gcggtgagac cctggtgcag cgtgaaggtg
     4561 gtcgcgttga tcgtggcggt gtccatcgcc tcgctgaacg tcgcggtgat cgaggcgttc
     4621 cgtgcgacgc cgaccgaggc gctgccaggc ccggtcgaag tgaccgtcgg gaccgtggtg
     4681 agggggttgg tcgggttggt gctgttgttg caggcgccgg ccgcgagacc cagggcgacg
     4741 agcgcgccca gcatgagggt tcggggggac ggcgttcggg gggaccgcac gggcgtggtg
     4801 atctggtaca tggagagtgt ccttggatcc ggagatccgt tcggggagat gagctacccg
     4861 tcaacaatag gactcatcgg ggccccaccc catcgcccag aggggtgaat tcgtgagtac
     4921 tccttttggg tgagggcgac gaaaaggccg cgcggatagc gcggccgggc acctgactcc
     4981 cccttatgcc acgttcggcg ttacgcctcg ccctcccggt gggagtaggc cgcgatctgc
     5041 agccgggtgt gcagggcgag cttctccatc acgttccgga cgtggctctt cacggtgtgg
     5101 gtggcgatgt tcagcttgga agcgatctcc ttgttgctgg atccctcccc gatgaggtcg
     5161 atcacctcgc gctcgcgggg ggtcatgcgc acggcctcca cggcccgcgt gggctccaac
     5221 gcctcgactt cccgcgcgat ctgcgcaaag agcgattcgg tcatgcgcgg cgggagcacc
     5281 ttcacccccg cggcgaccga ccggatcgtc gccacgaact cgtcgaaggt cgcatccttc
     5341 aggacgaagc ccgagacccc gatgttgacg tattccttga tctcctcgtt caccggcagg
     5401 acgtccatca tgatgatgaa ggcctcggga tgctcttcca ggagccccgc cgccacgcgc
     5461 aggctgtcct gatcccgcag cccgacgtcg aggaggagga catccggctt cgattcggcg
     5521 aggaaggtcg gatcggccgt gtcgagggag atgacccgga ggtcagggag gcggcccagc
     5581 atcgccgaga gcgcctcgcg caccagccgg ttgtcctcga tcacggcgac ggtaatgagc
     5641 cgcagcggcg gcttgcgagc ccgggacgcg ccggggcgcc gcgaggtgga tttcacggac
     5701 ggatggcacg ggttgtgact gcggtaattc gatgcatctc ccccgataag gtgggtctgc
     5761 ctcccttgcg ctacgcctcc ccgccgtgct cctccagcca ccgcagcatc tgcttcacgc
     5821 tcccggtggc gagcgcgatg ctggtgtcgg cggcgccgta gtagaggttg acggtatcgc
     5881 cgtcgtcgcc gatggtgcag ccgcagggga aggcgacgtt gcccacgtcc cccgcccgct
     5941 catacggcgc ttcggggccg aagacccaca tatccccccg ccgcaggcac cgctcagggt
     6001 cctcgaggtc gaggagcgcc aggcccagcc ggtagaggct ccccgacgcc gtgtggcgca
     6061 cgccgtggta gatcgtgagc caccccgccg gcgtctcgat caagggcggc gagagcccga
     6121 ccttgttggc gtcccaccac gccccccgcc gcgcctgcag caccagcttg tgcccgcccc
     6181 agttgcgcag gtcgggggag tatgagatcc agacgtgcgc ccccgagtcg tgcatcggcc
     6241 ggtggatgag cgcgaagttg ccgttgaccc gccggggcag gagggcggcg tccttgtcgt
     6301 cgggctgcat cacgagcccg taccgctcga aggtccggaa atcctcggtg agcgcgagcg
     6361 ccacccccgg ccccccgcgg ctgaacgcgg tgtaggcgac ggcgtacttg ccgagttccg
     6421 gcacgtaggt gatgcgcggg tcttccacgc cccagaattc ctcgggatag cgctcggcgt
     6481 cgggctggaa ggtgggggtc cggtcgatga cccagccgtc gatgccgttg gccgatcgcg
     6541 ccgcggtgag gtgggagtgc ccgcggcggt cctccacccg gcagaggagg agcgtggtcc
     6601 cgtccttgag gcgcaccgca ccgggattga agacggtgtg cgcggggtag ggccagtcgg
     6661 cggcggtcag gatcgggttg gcggcgtggc gcgtgaagag cacctcgtac tgctcgcgcg
     6721 ggctcacgcc cgcaccgccc gcatgtccca cagcgcctgc aggaacgaga gggtcgactc
     6781 ggcgccctgg ttctcgttca tccggtcggc gtggaggccg tcgcggcacc cgccggtgga
     6841 ggagtcgtac accgggagct gcaggtggtt ctgccccagg aaccagccga acgcggagtc
     6901 agcgtagcgc ttccagcgcg cgtccccggt gacgcgatgg gccgagaggc aggccgacac
     6961 cattccgcac gcttccaccg gttgctggtc gaagcgcgcc accgccgctc cccgccggta
     7021 gaaaccgttg gagcccaccg gcgcgaagta gccgtcgggg gtgtgctgca gcgtcccgag
     7081 ccactcgagc gcccgggtgc ccgccgccac catgtccggc cggcccatcg ccgcgcccga
     7141 cacgatcagc gcctgcggga gccgggcatt gcagtaggtc atatggtcct cgaaccaggg
     7201 ccagtcctcg gcgctggtgc gggtgtagag gccgaagagg cgctccgcca cgcgcgcccg
     7261 cgtggcctgg acgccggtat cgccctggaa ggcgcggagg tattcgtcga tgccgaggag
     7321 cgcgtaggcc caggcgcggg ggctcgagag ctcgtccacg gcgttgagcg ccgcgtggaa
     7381 gagcccgctg ctcaggctcc ggcgccccgg atcgacggcg cggcccacca cggtgccgag
     7441 ggcccagagc gcccgtccgt ggctgtcctc ggagccgcac agctcgcccc aggtgcgggc
     7501 gaaggtgagg aagttccgga accggccggt ggccgggtgg aaggcgtggc tcacgaaggc
     7561 gaggtagcgg gaggcgagct tccggagcgt cttccggtcg accgtccccg actcctcgag
     7621 cagcaccatc agcatcagcg cccgcgcgtt gtcgtcgagg cagtacccct cggcgtagcg
     7681 cggcaccacg aacgtcgcgt gctgcaggat gcccgtgtcg tcggtgagcc gttccaggtg
     7741 gtcgaggtcg agctcgggaa ggctcgccgg ccgctccgcc agcgtgcggg cgtggaagga
     7801 ggtgcgccgc cgctcggcgt gggccagcac cgcgcgctca aggctcccca cgtgacgccg
     7861 ggccaccgca ccccacgcca tcccgcggcc gtgctccgcg gcgcggtggc gcatcgcggc
     7921 ggtccggcgc gggtcgtcga ggagcccggt gacctcccgg gcgatcgccc ccgcatcgcg
     7981 ccacgggacc aggatcccgc gcccctccgc caggagctcg gtggcgtagc ggtacggggt
     8041 cgagatgacc gccttcccgg cgcccacggc gtaggccagg gtgccggagg tgctctgttc
     8101 cgggttcaag tacggcgtca cgtagatgtc ggcggcggag aggaactccc ccagttccgc
     8161 ctggctcacg aaccggttgt ggaagatgat gctggcgtcg acgcccaggc gctgggcccg
     8221 ggcctcgagc atcaggcggt agctctcccc cgacccttcc agcacgtggg ggtgggtggc
     8281 gccgaggacg atgtagaccg tgtcggggtg ttccgcgagg atggcgggga gggcgtcgat
     8341 gacgtgctcg atccccttgt ccggcgagag gaggccgaac gtgaggatga ccgacttgcc
     8401 ggcgacgccg aggcggtcct tgtcgccctc ggcgaacatc acctcgggga tcccgtgggg
     8461 gatgacgtcg atcttcccgg gatccacgcc gtgcacctgc gtgaggagcg tggcgctgtg
     8521 ccggctcatc accaccaggc gctcggagag ccggcagatc tcgtccatca cccgccgctg
     8581 gccggggtcg gggtcggcca ggatggtgtg cagcgtggtg acgatcggca tccggagctc
     8641 ggccaggagc gggaggatga gggcgcccgc ctttccgccg aagatcccgt actcgtgctg
     8701 cagcgagacc gcatccaccg tggagacgtt gaggtagtcc accgcgcggc ggtaggaggc
     8761 gacgtcgttc tcctcgatct cgaagcgcac catcggtggg taggcgtggc gggggccggc
     8821 gtcgttcatc gccacgacca ggcagtcgag ggcggggaat tcgtcgctga tcgcggcgcc
     8881 caggtcggtg gtgaatgtgg cgatgccgca cttgcggggg aggtggttgc cgaggagggc
     8941 cacctgccgg agtgccgggg tgcgggattg ctgctcgacg ctgcgcgtgg tggaccacgc
     9001 gtgcggggtc gaggatggag cgagcggacg agcgagagcc acgggaccag cttgctggca
     9061 ctactgggga aggagaccgg cactgcgggg atccgatgtc cgccaacgtg gcgaggccgg
     9121 cattcggctt cggcagggat cggtcggcgc cgcgatcggt ccagagggat ggcgaaggaa
     9181 agatagtcgg tcggacgggc gggtggggcg gatggggcgc gtgaacagcc gcgtgaggcg
     9241 caggggcgca gagacgcagg ggacctcgtc ccacaccggg gtcatcctga gggagcggag
     9301 cgaccgaagg atccctcggc ccacgtctca cccccctctc ctgcgtcgcc caagcctcac
     9361 ccccgacccc tctccacgtt cgtggagagg gggggaggat ggtgcggagg cgggctagaa
     9421 cttgatgcgc agtcccgccg acaccggcca gacccaggcg ttggtggtgt tgacctcggc
     9481 cttcttggag aaggcgatgt cgccctgggg ggagatcacg aacccgacgc tgcggttgaa
     9541 gttgtaggtg atcttggcgc cggcgttgat ggcgaagtac gtcttggtgc cggcggcgcc
     9601 gaacccgtcg aaggtgaccg cgccggcacc gaggttgacc atcgcgtccc agccgtgctc
     9661 gcgctcgccg gtcagggagt agccgagctt ggccatgtaa tgcagcgtct tgatggtgac
     9721 gctgttggtc gccttgtcct tgtggtgccc gtagtcgaac tcggccatca gggtccagtc
     9781 cgggtcgagc cggtagccaa aggtggcgcc gaacatcgcg ccggtcttga tgtcgttggc
     9841 gatgtcgaag gtcgggatcg cggcaccgcc gaggccttcg atgaagaacg ccgggttcgt
     9901 gctcgacgac ccctgcgcct gggccggccg ggccgcgagc agggtcgagg cgatgagcac
     9961 tgcgaaggag agacgtgcgg ctgacatgga ggggctcctc agggggtaaa ggggaatgga
    10021 gggctccggg agtgcgctgc cgcgcgccat ccacgcgtgt cgcgacgccg ccgcgcacgg
    10081 ctcgcccacc caccagcggg atcgacacca ccgctggccg ccggggggag gagcaactcg
    10141 cgggccagtt ggaggcgcag ggacgcagag gcgcagggac gcgtgggggt cgcctgcggc
    10201 gacccaagcc tcaccccccg ggccctctcc atcgctgatg cgatgatggg aggcacgccg
    10261 tgcatcgggg tgtcatcccg agcgaagcga gggatctcaa accgatcgat gggtccagga
    10321 cgccgctgct gtcccggggc aggccgggca tcgggttgag atccctcggt cgctccgctc
    10381 cctcgggatg actccgatcc ccaccgacgt ccgcctcacc tcccctcctg cgccgcccaa
    10441 gcctcacccc ccgaccccac cggcgcctga aggcgcctct cggcatcgcg ccctgaaggg
    10501 cttcactcat catcgcgccg gatccccagg cgggcggcaa gccgctggtc cgattcggct
    10561 ggcgccgttg tcacaccccg gcggcatttt gggcggcggt acggcggtca ggcggatgca
    10621 gttcacgcgc cccgccttat gcgtcccatg cgtcttatgc gtcttatgcg cctcatgcgc
    10681 cttatccgtc ccatgcgtct tatgcgcctc atgcgactta tccgtcccat gcgtcttatg
    10741 cgcctcatgc gccccatgcg cctcctccac ccatcgaccg accatgctcc tcctgaccac
    10801 caatccctgg cgccgcctgc tcctccccac ccgggcgggg taccccctgc cgctgaagag
    10861 ccgggcaccg gccaacaacg agatcgtcct cttggcacgg gtgcgccgca ccccggcggg
    10921 gcgggtgcac gtggtgcaca cggcgctgcg cgcggtcgcc gtccgcctca agccgctctc
    10981 cccctggcgc ggacgctcgc ggtggatcat ccgcgcggcg cactggaagc tggcccagcc
    11041 cttccagctc gaggggatcc agctcacgcc gtcggagtac cgctcccgcg gcgccctcgt
    11101 ccccctcgac ccgacggatg tcgacgagat ctac
//
LOCUS       DASUAP010000148         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_412552, whole genome shotgun sequence.
ACCESSION   DASUAP010000148 DASUAP010000000
VERSION     DASUAP010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412552"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFU45_03565"
     CDS             complement(<1..206)
                     /locus_tag="VFU45_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEU5358176.1"
                     /translation="MAHKQLVFRSAAREKVLRGAAALADLVRVTLGPKSKCVLIEKKW
                     GQPVVCNDGVTIAKEVDLSDPEENL"
     gene            complement(216..443)
                     /locus_tag="VFU45_03570"
     CDS             complement(216..443)
                     /locus_tag="VFU45_03570"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEU5358177.1"
                     /translation="MGSSDSVIRAMVGLALIVTAAALSSVWMASAALVLVALVLLYTA
                     LTERCPMYHLFGWNTRPEPSPPTTPEPHAHG"
     gene            605..952
                     /locus_tag="VFU45_03575"
     CDS             605..952
                     /locus_tag="VFU45_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358178.1"
                     /translation="MTRTPAHHGDLDAILQLEKDLTNRVVEARTEAERILREAQADAA
                     RLDAGLEEELARRIQELDARLLAEREQRLAEVAATARQRQAAWDAVDDATVRRAAGVV
                     VDALVAGPRGARR"
     gene            949..2841
                     /locus_tag="VFU45_03580"
     CDS             949..2841
                     /locus_tag="VFU45_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HEU5358179.1"
                     /translation="MIIAMAKVRVMGPRDRLPEVLGVIQDVGVLHLAAPAADSAVTSV
                     VPTARENHERRNLRRLLDDVEWDLGHLGSGPGGRAPVAETAAPEPVEWARTAARLRRR
                     LERMDERLIQLDEERSLILKYRSFFDAFRSLLEAGQRWPHTTAYHVLLRSGDRDALPR
                     LRQALGHVLDNAFEMYDRPLESGETAVLVIVPADHAANLERLFAESRVEEIPVPAAYG
                     GGTLANAIPEMLARLKVIPRERDALGREREELARAHRAELERARAWLNDRRAHLDALA
                     LSGVTSHAFVIEGWLPAGSRAGFEKRIHAACGREVVVSEESREEWTSEEAPVVLSNPR
                     LFRPFEAVLRLMPLPKYGTLDPTPFVAVFFPAFFGLMVGDVGYGLILGLLALVVRTRT
                     RTGTLLRSVAEIAGACALFAVIAGVLFGELFGDLGSRWLGLKPVLFRREEAILPFLIL
                     AISLGAVHILLGLILGVVSSRAHPRQAVGRGISAAMLVGVIVALLAVLEVLPRGLLTP
                     TVIGLLVAFPLLILAEGITAPIELLSALGNILSYARIMALGVASVMLAVVANQMVGAM
                     GSVAVGVTFALLFHLVNFAIALFSPTIHALRLHYVEFFGKFYSAGGVRYAPFGRWVAG
                     AGRRAA"
     gene            2864..3091
                     /locus_tag="VFU45_03585"
     CDS             2864..3091
                     /locus_tag="VFU45_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358180.1"
                     /translation="MNLVWIVFGAALAVAVPAIATAWAQSRIGSAIAASMAEKPELST
                     TAILMIAIPETMVILGFVVALVILLRAGGPG"
     gene            3096..3680
                     /locus_tag="VFU45_03590"
     CDS             3096..3680
                     /locus_tag="VFU45_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358181.1"
                     /translation="MAFADLLHALTARADGEIAALLNSAREQAQVIRRESDQRRAERS
                     ATALRDRTRAVTVDTERGVAEAVRLERHNELAARIRARDHVLSSARRMLADADLRPDY
                     QAAVSGRLAAAAAVLGDAPARLRCAPALAPVLNPLALTYSNLRLEPDASVAGGFVIES
                     DDGRVMVDEVLEHRLEGDAEALGQLALRSLEVEP"
     gene            3677..>4445
                     /locus_tag="VFU45_03595"
     CDS             3677..>4445
                     /locus_tag="VFU45_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEU5358182.1"
                     /translation="MTLWVDVVARARGLRTHRLSASVMTALRAAPDLTAFGRLLKDAG
                     FLVEEGERHAAALDVAVRRRAAAELRILARWCGDRAAELPIVLEDEDRRSLRALLRAT
                     LQRQPVELRVRGLVPTPTLPLRALSELAHAQSPADVVTLLTIWNHPFANALGPSVHAT
                     TPDPLAMDLALDRAWAGRAVAGARRGGPELERFVADAVDLTNATTVLTLAGTTGTHAP
                     EFYLEGGHRLSRAAFTALLARDRLAASDALSDLYHATP"
BASE COUNT          653 a         1564 c         1478 g          750 t
ORIGIN      
        1 agattctctt cggggtcgga gagatcgact tccttggcaa tcgtcacgcc gtcgttgcag
       61 accaccggct ggccccactt cttctcgatc aggacgcatt tggacttggg gccgagcgtg
      121 actcgcacca ggtccgcgag cgctgccgca ccccgcaaca ccttctcgcg ggccgccgaa
      181 cggaatacca gctgcttgtg agccatgggt ccccctcatc cgtgggcgtg cggctcgggt
      241 gtcgtcggtg gagacggctc cggccgggtg ttccagccga ataggtgata catcgggcag
      301 cgctcagtga gcgccgtgta gagcaggacc agcgcgacca acacgagtgc ggccgacgcc
      361 atccagaccg acgacagtgc tgcagcggtg acgatcaacg cgagtccgac catggcgcgg
      421 atcaccgagt cgctgcttcc cacgttgcga tccatacgac tccccctgat tggtccaaga
      481 accaggatca tcgggcgaca atgagaactg gatggagcgg tgatgaagaa accgactggg
      541 cgggtgaaga gatcttccgg gctccttcat cgagtccagc cgaaccctca gacacccttc
      601 atccatgacg cgtaccccgg ctcatcacgg tgacctcgac gccatcctcc agttggagaa
      661 ggacctgacc aaccgggtgg tggaggcccg gaccgaagct gaacggattc tccgggaggc
      721 acaggccgac gcggcgcgcc tggatgcggg cctcgaagag gaattggctc gccggattca
      781 agagctggat gcccggctcc tggcggaacg ggagcagcgg ttggcggagg tggcagcgac
      841 ggcgcggcag cgccaggcgg cctgggatgc ggtcgacgac gccacggtgc gtcgcgccgc
      901 cggcgtcgtc gtggacgccc tggtcgccgg cccccgggga gcgcgccggt gatcatcgcg
      961 atggccaagg tccgcgtgat ggggccgcgg gaccgcctgc ccgaagtgct gggcgtgatc
     1021 caggacgtcg gcgtgctcca tctcgccgcg cccgccgctg atagcgcggt cacgtcggtc
     1081 gtgcccaccg cccgcgagaa ccacgaacga cggaacctca ggcgtctgct cgacgacgtg
     1141 gaatgggatc tgggccatct cggatccggt ccaggcgggc gcgccccggt ggcggagacg
     1201 gcggcacctg aaccggtgga atgggctcgc acggccgcgc gtcttcgccg gcgcctcgag
     1261 cggatggacg agcggttgat ccagctcgat gaagagcgga gtctgatcct gaaataccgc
     1321 tccttcttcg acgccttccg gtccctgctg gaggccggac aacggtggcc acacaccacc
     1381 gcgtatcacg tcctgcttcg atccggcgac cgggacgcgc tcccgcgcct gcggcaagcg
     1441 ctcggccacg tcctggacaa tgccttcgag atgtatgacc gccccctcga gtccggcgaa
     1501 acggccgtgc tcgtcatcgt accagcggac catgccgcga atctggaacg actgttcgcc
     1561 gaatcacgcg tcgaggagat accggtgccc gcggcctacg ggggcggcac cctggcgaac
     1621 gccattcccg aaatgctggc ccggctgaaa gtcattccgc gcgagcgcga cgcgctgggc
     1681 cgcgaacgcg aagaattggc ccgcgctcac cgggcggaac tggagcgcgc ccgtgcctgg
     1741 ctcaacgacc gccgggccca cctcgacgcc ctcgcgctga gcggtgtcac gagccacgcc
     1801 ttcgtgatcg agggctggct ccccgccggc agccgcgccg gtttcgaaaa gcgaatccat
     1861 gccgcctgcg gtcgcgaggt ggtcgtcagc gaggaatccc gggaagagtg gacgtccgag
     1921 gaagctcccg tcgttctgtc gaacccccgg ctgtttcgtc cattcgaagc ggtgctccgg
     1981 ctgatgccgc tccccaagta cggcaccctc gaccccacac cctttgtcgc cgtcttcttc
     2041 cccgcgttct tcggactgat ggtcggtgac gtggggtacg gcctgattct cggccttctc
     2101 gccctcgtgg tcaggacgcg aacccggacc ggcaccctcc tgcgttcggt ggccgaaatc
     2161 gcgggcgcct gcgccctctt cgccgtcatc gccggggtgc tcttcggcga gctctttgga
     2221 gacctcggat ccaggtggct cggcctgaag ccggtcctgt tccggcgcga ggaggccatt
     2281 ctgcccttct tgatcctcgc catctccctg ggtgccgtgc acatcctcct ggggctcatc
     2341 ctcggcgtgg tgtcgagccg cgcccacccc cggcaggcgg tggggcgcgg aatctcggcg
     2401 gccatgctgg tcggcgtcat cgtggccctg ctcgcggtgc tggaggtcct gccgcgtggg
     2461 ctcctcacac ccaccgtcat cgggctcctc gtggccttcc ccctcctcat tctggcggaa
     2521 ggaatcaccg cccccatcga gctcctgtcc gcactgggca acatcctgtc gtacgcccgt
     2581 atcatggcgc tcggcgtggc atcggtgatg ctcgccgtgg tggccaatca gatggtcgga
     2641 gccatgggca gcgttgcggt aggcgtgacg tttgcgctgc tctttcatct tgtgaatttc
     2701 gcgatcgcgc tattcagccc gaccattcac gccctccgtc tgcactatgt cgaattcttc
     2761 gggaagttct acagcgcggg cggcgtgcga tatgcgccgt ttggacgttg ggtcgcgggc
     2821 gctggacggc gcgccgcgta agccgctcaa gaaaggtgga cccgtgaacc tcgtctggat
     2881 cgtcttcgga gccgcgctgg cggtggccgt gccggccatc gccaccgcct gggcacagtc
     2941 ccggatcggc tcggccattg cggcaagcat ggccgaaaag ccggagctct ccaccacggc
     3001 catcctgatg atcgccatcc cggaaacgat ggtgatcctc ggattcgtcg tcgcgctcgt
     3061 catcctgctc cgggccggcg ggccggggtg atggcatggc cttcgcggat ctgctccacg
     3121 ccctgacggc tcgcgccgac ggggaaatcg cggcattgct caactcggcc cgcgagcaag
     3181 cgcaggtcat ccgccgcgaa tccgaccaac gtcgcgccga gcggtccgcc acggcgctcc
     3241 gggatcgcac gcgcgccgtg accgtcgata cggaacgcgg cgttgcggaa gcggtccggc
     3301 tcgagcggca caacgagctg gccgcgcgca ttcgcgcccg ggatcacgtg ctctcctcgg
     3361 cccgcaggat gctcgcggac gcggatctgc gccccgacta tcaggccgcg gtttccggcc
     3421 ggctcgccgc cgccgccgcc gtcctcggtg acgcgcccgc ccggctgcgc tgcgcgccgg
     3481 ccctcgcccc cgtgctcaac ccgctcgcct tgacgtactc caacctccgg ctggaacccg
     3541 acgcgagcgt ggcaggaggc tttgtgatcg agagtgacga cggccgtgtg atggtcgacg
     3601 aggtcctcga gcatcgactc gagggagacg ctgaggccct cggtcagctc gcccttcggt
     3661 cgctggaggt ggagccatga cgctctgggt ggacgtcgtg gcccgggccc gcggccttcg
     3721 aacccatcgc ctgtctgcga gtgtgatgac ggccctgcgc gccgcgcccg atctgacggc
     3781 cttcggtcgt cttctgaagg acgccggatt ccttgtagag gaaggtgagc gtcacgcagc
     3841 tgccctggac gttgcggtcc gacgccgggc agctgccgaa ctgaggatcc tcgcccgctg
     3901 gtgcggggat cgcgccgccg agcttccgat cgttctcgag gacgaagatc gccgatcgct
     3961 ccgcgctttg ctccgcgcaa ccctccagcg gcaacccgtc gagctgcggg tgcgcggtct
     4021 cgtgccgact ccgaccttgc cgctgcgagc cctgtccgaa ctggcccatg cgcagtcccc
     4081 cgcagatgtg gtgacccttc tcaccatttg gaaccacccc ttcgcgaatg cgcttggacc
     4141 gtcagtgcac gcgacgacgc ctgatcccct ggcgatggac ctggcactcg accgcgcctg
     4201 ggcgggacga gcggtggccg gagcgcgccg gggcggcccc gaattggagc ggttcgtcgc
     4261 cgacgccgtc gacctcacca acgccacgac cgttctgacg ctggccggca ccacggggac
     4321 gcacgccccc gagttctacc tcgaaggagg gcaccgcctc tcgcgcgccg cgttcacggc
     4381 cctgctggcg cgggaccgtc tggccgccag cgacgcgttg agtgacctct atcacgccac
     4441 gccct
//
LOCUS       DASUAP010000149         5087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_208235, whole genome shotgun sequence.
ACCESSION   DASUAP010000149 DASUAP010000000
VERSION     DASUAP010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208235"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VFU45_03600"
     CDS             <1..587
                     /locus_tag="VFU45_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358183.1"
                     /translation="TLAQGFGLLKDALSGLTTPAPHETVRQRMLALAGKEDPLLDLPR
                     FDRFLRQAHDAEVAEVRKVGDGAFDIAPFGSVGGPPARLERPPQQPSRVVEPAPVAEP
                     PAAPGPVPVAQVQALRFRRGSKVGMRPAEVPLVGVVAVEVEEAPAAKGRKRGGRGAKP
                     AATQPAEPGTEAADAVPASAPRRRRGGRSKKAAS"
     gene            595..1221
                     /locus_tag="VFU45_03605"
     CDS             595..1221
                     /locus_tag="VFU45_03605"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU5358184.1"
                     /translation="MAPTLRRLTGRAGRPLPVPFFERPSEVVARELIGAIVEVRRGDT
                     LAFGRIVETEAYLGADDPASHAFRYRRHAGNASLYLPSGTWYVYRSYGIHWCANLIAG
                     GRPAGSAVLLRALEPLDGLAAMRRRRGGVEDRLLANGPGKLCQAFAIDRSLDGRPMQR
                     SWARVFADAPVSDITVTPRIGISKAVDWPLRFVATGTSWASRPTARRS"
     gene            complement(1264..1965)
                     /locus_tag="VFU45_03610"
     CDS             complement(1264..1965)
                     /locus_tag="VFU45_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358185.1"
                     /translation="MRMLSRSIALGLLAATVVSMPAWAQGSQRYTLYFGGEGGLMSVK
                     NGDAAGKRDFMATAGAHLLVTGARSGLLFSVDQTFGTNKTGTINSVVVDSAGNVTSAS
                     VAATYTGVRRYTLAVLLFPVQHSIIQPYVGFGGGIQHATGITPGGHVPAALGTQGFLT
                     AIGGLEFRIAGLSAFGQGQITSAPSAHTIRSDLINGSTEYDTGSLFQEASYSVMAGIR
                     LSLGNARSSGGPSSY"
     gene            complement(2194..3393)
                     /locus_tag="VFU45_03615"
     CDS             complement(2194..3393)
                     /locus_tag="VFU45_03615"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEU5358186.1"
                     /translation="MNGTLEGRIEAELAGFKRAGVYKRLNYLESAQGPVVRMEGRGEV
                     VVLSSNNYLGLADHPEVVRAGKDALDRFGAGTASVRFICGTFTVHRELEQACARLVGA
                     PASLSFVSCWNANEAVPGTLLTEQDLVLSDQLNHASIIDGIRLAKAITKCQTGVYAHG
                     DLTDLDEKLAAAKDRRVKLVISDGVFSMEGAICKLPDLLEVCRRHGAVLMLDDSHATG
                     VLGRTGRGTAEHFGLVGEVPLITSTLGKALGGAAGGFVAASEAVCDYLTQRARPQLFS
                     NALPPTVAASALRSIEVLEREPARVETLRQNASYFREQLLGLGFKPLAGETPIIPVIL
                     GETAKAIQMSEALLDEGVFVTGFGFPVVPQGQARVRCQISAGHTRAHLDRALDAFKKA
                     GRKLGLI"
     gene            complement(3390..4430)
                     /gene="tdh"
                     /locus_tag="VFU45_03620"
     CDS             complement(3390..4430)
                     /gene="tdh"
                     /locus_tag="VFU45_03620"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEU5358187.1"
                     /translation="MRALVKESAGPGMVVKDVPVPRIGPSDALIQVRHAGVCGTDLHI
                     WEWDAWASGRLKPPVVIGHEFAGVIAELGAEAKAGGLLAVGDLVTAEGHVVCGHCHPC
                     RHNQFHVCERTQIIGVDRDGAFAEFIAMPASNVMKLDGIPTEIGAIMDPIGNAVHTTL
                     EGMVAGNSVFVLGCGPIGCFAVGIAKAAGASVVIASDFNPVRRELARKMGADHTLDPA
                     AVDIPARVRALSGTGGADLICEMSGHPTGHQQAFASVRPGGRINFLGTPAKSTEVNFA
                     RDIIFKGLTLYGVTGRRMYETWQQMGQLLRGGRFDPRPVITHRFPMEGIAEAIGRIKD
                     GQAGKVILEIAQ"
     gene            complement(4552..4719)
                     /locus_tag="VFU45_03625"
     CDS             complement(4552..4719)
                     /locus_tag="VFU45_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358188.1"
                     /translation="MNAAGITTEQEPIGIIIHDGGERRKPAAIWAYMWAADEDETTDH
                     WHEHMPVDHAA"
     gene            complement(4815..>5087)
                     /locus_tag="VFU45_03630"
     CDS             complement(4815..>5087)
                     /locus_tag="VFU45_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358189.1"
                     /translation="QIVVLDSGEVGALAESDLRMFIGSLQSRSATVGVHVLLGHAGRV
                     AELRAWYAGWRHLPAEDHGAVLVPPTCQNDSDTPESLQHTLLDARD"
BASE COUNT          746 a         1793 c         1761 g          787 t
ORIGIN      
        1 tgacccttgc gcaaggcttc ggcctcctca aggacgccct ctccgggctc accaccccgg
       61 ccccgcacga gaccgtccgc cagcggatgc tggccctcgc cggcaaggag gatccgctcc
      121 tcgacctgcc gcgcttcgat cgtttcctcc ggcaggccca cgacgccgag gtggccgagg
      181 tgcggaaggt cggggatggc gcgttcgata tcgcgccgtt cggttcggtg ggcggcccgc
      241 cggcgcggct ggaacgcccg ccgcagcaac cctcccgggt cgtggaaccg gcgccggtgg
      301 ccgagccccc cgccgctcca ggaccggtcc ccgtggccca ggtccaggcg ctccgctttc
      361 gccgcggctc gaaggtcggg atgcgtccgg ccgaggtgcc gctggtcggc gtggtggcgg
      421 tcgaagtcga ggaggccccg gccgcgaaag gacgcaagcg cggcggccgc ggggccaagc
      481 ctgcggccac gcagcccgcc gagcccggga cggaggcggc cgatgcggtg cccgcgtccg
      541 cgccgcgccg tcgtcggggc ggccggagca agaaggccgc atcataggcc catcatggcg
      601 ccaacgctcc gacgactgac gggacgggcg gggagacccc tgcccgtccc gttctttgag
      661 cgtccctccg aggtcgtcgc ccgcgaactg atcggcgcga tcgtcgaggt gcggcgcggc
      721 gacaccttgg cctttggccg catcgtcgag acggaagcgt acctcggcgc ggacgacccc
      781 gcctcccatg ccttcaggta tcggcgccac gccggtaacg catcgctgta cctgccgtcc
      841 gggacctggt acgtctatcg gtcgtacggt atccactggt gcgccaacct gatcgccggt
      901 gggcggcccg ccgggtcggc ggtgctgctg cgcgccctgg agccgctcga cggcctggcc
      961 gcgatgcgcc gccgacgggg cggggtcgag gaccgcctgc tggccaacgg cccggggaag
     1021 ctctgtcagg ccttcgcgat cgaccggagt ctcgacggcc ggccgatgca acgctcgtgg
     1081 gcgcgggtct tcgcggatgc gccggtctcc gacatcacgg tcacgccgcg gatcggcatc
     1141 tccaaggcgg tcgactggcc gctgcggttc gtcgcgaccg gaacttcgtg ggcctcaagg
     1201 ccgaccgccc gccggtcgtg aagaccggcg ggcggtgaag gaaacggcgg aaggctccgg
     1261 cggtcagtaa ctcgacgggc cgccgctgct gcgtgcgttg ccgaggctca gccggatccc
     1321 ggccatcacg ctgtacgacg cctcctggaa gagcgagccg gtgtcgtatt cggtgctgcc
     1381 attgatcagg tccgaccgga tggtgtgggc ggacggcgcc gaggtgatct ggccctggcc
     1441 gaaggccgat agcccggcga tccggaattc gaggccgccg atggcggtca ggaacccctg
     1501 ggtgccgagg gccgccggca cgtggccacc cggggtgatc ccggtggcat gctggatgcc
     1561 gccaccgaac ccgacgtacg gctggatgat cgagtgctgg accgggaaga ggagcaccgc
     1621 gagggtgtac cgccggacgc ccgtgtaggt ggccgcgacg ctggccgacg tcacgttgcc
     1681 cgcgctgtcg accaccaccg agttgatcgt gccggtcttg ttggtgccga aggtctggtc
     1741 gaccgagaag agcaggccgc tcctggcgcc ggtcacgagc aggtgcgcgc cggccgtggc
     1801 catgaagtca cgcttgccgg ccgcatcgcc gttcttgacg ctcatcagcc cgccctcgcc
     1861 accgaagtag agcgtgtacc tctgggagcc ctgggcccac gccggcatcg acaccacggt
     1921 ggcggccaga agcccaagcg cgatcgaacg gctcagcatc ctcatcggtc ttcctccgtg
     1981 atggtccggt cccggcgcca cctgacgggc agcaagtcgt tgtgggagtg gcacttccgt
     2041 ggccgagtcc ccgccggcga ccccgggggg ataccccata caataggaag accccgccaa
     2101 aaaagctagc cccgaatggc cgggtgggag gtaccctagg ccgttcgggg ataagcagtt
     2161 aacggaccga actacgaaac tacttgaggt gcttcagatc aggcccaact tccggcccgc
     2221 cttcttgaac gcgtccaggg cccggtcgag gtgcgcccga gtgtgtcccg cggagatctg
     2281 gcagcgtacc cgcgcctgtc cctgcggcac caccggaaag ccgaatccgg tcacgaacac
     2341 tccttcatcg aggagcgcct cgctcatctg gatggccttg gcggtctcgc ccaggatgac
     2401 ggggatgatc ggcgtctcgc cggccagcgg cttgaagccg agcccgagga gctgctcgcg
     2461 gaagtagctc gcattctggc gcaatgtttc cacccgcgcc ggctcccgtt ccagcacctc
     2521 gatgcttcgc agcgcgctcg ccgccacggt gggcgggagg gcgttggaaa agagctgcgg
     2581 tcgcgcccgc tgggtcaggt agtcgcagac cgcctcgctc gcggcgacga acccccccgc
     2641 cgcgccgccc agcgccttgc cgagggtcga ggtgatgagc ggcacctcgc ccaccagccc
     2701 gaagtgctcc gcggtgcccc gcccggtccg gcccaggacg ccggtcgcgt gggaatcgtc
     2761 cagcatcagc accgccccgt gcctgcggca cacctcgagg aggtcgggga gcttgcagat
     2821 ggccccttcc atcgagaaga cgccgtcgct gatcacgagc ttgacccggc ggtccttcgc
     2881 ggccgcgagc ttctcgtcga ggtccgtgag gtcgccgtgg gcgtacaccc cggtctggca
     2941 cttggtgatg gccttggcga ggcggatccc gtcgatgatc gacgcatggt tgagctggtc
     3001 ggacaacacc aggtcctgct cggtcaggag cgtgcccggg accgcctcgt tggcattcca
     3061 gcagctcacg aagctgagcg acgccggcgc cccgacgagc cgggcgcacg cctgttcgag
     3121 ctcgcggtgc acggtgaacg tcccgcagat gaatcgcacc gaggcggtcc cggcaccgaa
     3181 ccggtcgagc gcatccttgc cggctcggac cacctcgggg tggtcggcga gcccgaggta
     3241 gttgttggat gagagcacca ccacctcgcc ccgcccctcc atccgcacga ccggcccctg
     3301 cgcactctcg aggtaattga gccgcttgta caccccggcc cgcttgaacc ccgcgagttc
     3361 ggcctcgatg cggccttcga gcgtcccgtt cattgtgcga tctccaggat caccttgccg
     3421 gcctgcccgt ccttgatgcg ccctatggcc tcggcgatcc cttccatcgg gaagcgatgg
     3481 gtgatcaccg gccgggggtc gaaccggccg ccccgcagca gctgccccat ctgctgccac
     3541 gtctcgtaca tccggcggcc ggtgacgccg tacagcgtga gtcccttgaa gatgatgtcc
     3601 cgggcgaaat tgacctcggt gctcttcgcg ggcgtgccga ggaagttgat gcgcccgccg
     3661 ggccgcaccg aggcgaacgc ctgctgatgg ccggtcgggt gcccgctcat ctcgcagatc
     3721 aggtcggcgc cgccggtgcc cgaaagcgcc cgtacccgcg cggggatgtc cacggcggcg
     3781 gggtccaggg tgtggtcggc gcccatcttc cgcgccagct cgcgccgtac cgggttgaag
     3841 tcgctcgcga tcaccaccga cgctcccgcg gccttggcga tcccgaccgc gaagcacccg
     3901 atcggcccgc agccgaggac gaacacggaa ttccccgcga ccatcccctc gagcgtggtg
     3961 tggaccgcgt tgccgatcgg gtccatgatg gcgccgatct cggtggggat gccgtcgagc
     4021 ttcatcacgt tcgacgccgg catcgcgatg aactccgcga acgccccgtc ccggtcgacg
     4081 ccgatgatct gggtgcgctc acagacgtgg aactggttgt ggcggcacgg atggcagtgc
     4141 ccgcacacca cgtgcccttc cgccgtgacg aggtctccca ccgcgaggag gccgccggct
     4201 ttcgcctcgg cgccgagctc cgcgatcacg cccgcgaact catggccgat caccaccggg
     4261 ggcttgagcc gccccgacgc ccacgcatcc cactcccaga tgtgcaggtc cgtgccgcag
     4321 accccggcgt gccgcacctg gatgagtgca tccgaagggc cgatccgggg gaccgggacg
     4381 tccttgacga ccatgccggg tccggcggac tccttgacga gcgctcgcat tggcgatccg
     4441 atgggataag accggcggga atataggcgg ggcgatgggt gcgcggtgag cctcaaaacg
     4501 aagacccccg agcgggtggc tcgggggtcg gcggactggc gcggggaccg gtcaggcggc
     4561 gtggtcgacc ggcatgtgct cgtgccagtg gtcggtcgtc tcatcctcat cggcggccca
     4621 catatatgcc cagatcgcgg ccggcttgcg gcgctcccca ccgtcgtgga tgatgatccc
     4681 gatcggctcc tgctcggtgg tgatcccggc tgcgttcatg gtggtaccct cgatggtaga
     4741 tggactccgg ccatctccag aagcagggta cgtgccacga ggcatcatca attaagtggt
     4801 tgcagatccg atagttagtc ccgtgcgtcg agcagggtgt gttgaagtga ctccggcgtg
     4861 tctgagtcgt tctgacacgt cggggggacc agtaccgcgc cgtggtcttc cgccgggagg
     4921 tggcgccagc cggcatacca tgcccgcagt tccgcgaccc gaccggcgtg gcccagcagc
     4981 acgtgcacac cgaccgtcgc gctccggctc tgcagggatc cgatgaacat ccgcaggtcg
     5041 gactcggcca gcgccccgac ctcgccggaa tcgagcacca cgatctg
//
LOCUS       DASUAP010000150         5254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_821327, whole genome shotgun sequence.
ACCESSION   DASUAP010000150 DASUAP010000000
VERSION     DASUAP010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821327"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..3524)
                     /locus_tag="VFU45_03635"
     CDS             complement(414..3524)
                     /locus_tag="VFU45_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEU5358190.1"
                     /translation="MIRSVIAWALRNRFLVLLGTAAIAAAGVWAVMTTPLDAIPDLSD
                     VQVIVQTDFSDQAPQIVEDQVTYPIATEMLKVPGTRVVRGFSFFGQSLVYVLFEDGTD
                     IYWARSRVLEYLNGIRSRLPQGVEPTLGPDATGVGWVFEYTLQSDSLDLAQLRSLQDW
                     SIRYQLTAIPGVSEVASLGGFVKQYQVDIDPERLRAFRLSPMRVVEAIGAHNTDIGAR
                     VLEMAGREYMIRGLGYLRSTADIGDVVLSSTPSGTPVRVRDVANVHIGPAPRRGAADL
                     DGRGEAVAGIVVMRFGADAWRTIKAVKARIAEIQRTLPRGVRLVPAYDRSDLIRRAVD
                     NLRGKLLEESLIVAVVAILFLLHFRSALVAIVTLPLGILISFIVMRLIGVSANIMSLG
                     GIAIAIGAMVDAAIVMIENMHKHLERPGAEARPRWEVVRESAEQVGPALFFSLLIITV
                     SFLPVFTLELQEGRLFKPLAFTKTFAMAGAALLSITLVPVLMGYLIRGRIRSQDANPV
                     NRWLGRIYAPVIRWVLAHPWKVLAGAVAIVAVTALPWLRLGSEFMPPLNEGTLLFMPT
                     TVPGVSIGQAIGIMRYQDSVLKSFPEVATVLGKTGRANTATDPAPLDMYETMVVLKPA
                     AEWRAGMTYPRLVSEMDSAVRLPGVANAWTMPIKNRIDMLATGIRTPVGIKIYGPDLD
                     TLQQLGEAVERIVREVPGTRNVFAERNNSGNYVDIRVDRARAARYGLNAGDVHDAITA
                     TAGGYAAAMTVEGRERYEINVRYPPELRDNVDKLRQIVVPTAGGGEVPLGQIADIRTV
                     SGPMAVKTENAFPVTTVAVDIEGSDVGSYVNAARRLVQQRLTLPVGYRLEWSGEFESM
                     QRVRQRLWIVVPVTLTLIFLLLYLNFRGVAESLIVMASLPFGIVGGVWSLWLLGYNTS
                     VAVWVGFIALAGVAAETGVIMLIYLDEAYHRRAAAGAIVSVEDLAAAVHEGAVERLRP
                     KLMTVTAIMAGLAPILWSTGAGADVMKRIAAPMVGGMVSSTILTLVVIPAVYLLWRRG
                     EVRRKT"
     gene            complement(3521..4696)
                     /locus_tag="VFU45_03640"
     CDS             complement(3521..4696)
                     /locus_tag="VFU45_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5358191.1"
                     /translation="MSTRIGVIGVAAWWVATGCARTPAPPPAMAGMQMPVQAAAPTPG
                     AVRLTPAQASAIGVTYTTVVAGALTRPVRTVGEVAAPESSVVDVTVKVDGYIERMFVA
                     TTGAPVRRGQPLLSIYSPTLVAAQQELLTALGLVAGADSTDPGARSSAMSLLAATRRR
                     LANADIPAAQVARLEHTGEVTKTLTLTSPVSGVVLDKAVVEGQSVVTGAMLYRLADLS
                     TVWIDGAVFEQDLALLHTGARAQVEFASYPGRTWTGRITFISPVVDSMSRTAVVRVSV
                     ANREGLLKPGMYATLFLLAPLDPRLPHVPADAVVRTGERDMVYIARTDGTLEPQGVTL
                     GLRAGDQIQVLSGVRPGQRIVASANFLVDAESHLTTGGASMAGMPGMDAPPAAEVRP"
     gene            complement(4693..>5254)
                     /locus_tag="VFU45_03645"
     CDS             complement(4693..>5254)
                     /locus_tag="VFU45_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5358192.1"
                     /translation="LPSADSLAALALGGRAAIQAGAERIAAADAALGAAERERLPDFQ
                     VGLAYQQRPAFPDMVSLVLGVSLPIFPGRRQIPLRREMAAMQQMAQAEQLDLRNETTA
                     EVVEARARAERDRTLARLYQDRIVPQARAAVQSALAAYQVRKVDFATLVDDQLMVTRY
                     EIETWRLRADHEEAKADLATLTGALP"
BASE COUNT          808 a         1913 c         1761 g          772 t
ORIGIN      
        1 aacagtgaac gggctcccgg tcggaatttc gactcggagc ccttcttgtt cgtcaccctg
       61 agcgaagcga aggatcggcc cgccaccggc gcctccggat cgaatcccgc cctatccgtg
      121 ttcgcggtga acggtgaacg ggcaacagtg aacgggctcc cggtcggcat ttcgactcgg
      181 agcccttctt gttcgtcatc ctgagcgaag cgaaggatcg gcccgccacc ggcgcctccg
      241 gatcgaatcc cgccctgtcc gtgttcgcag tgaacggtga acgggcaaca gcgagcgggc
      301 tcccggcgca atctcaaccg ggagccctct cacctcttac ctctcacctc ttacatctta
      361 cctctcacct ctcacctctt acctcttccc tcttacctct cacctctcac ctctcacgtc
      421 ttacgtctca cctccccccg ccgccacagg aggtacaccg ccgggatgac caccagcgtg
      481 aggatggtgg agctcaccat cccgccgacc atcggcgccg cgatccgctt catcacgtcg
      541 gcgcccgccc cggtgctcca caggatcggg gcgagacccg ccatgatcgc ggtgacggtc
      601 atcagcttcg gccgcagccg ctcgaccgcc ccttcatgca cggccgccgc gagatcctcg
      661 accgacacga tcgccccggc ggccgcccga cggtggtacg cctcgtcgag gtagatgagc
      721 atgatgaccc ccgtctccgc ggcgacaccg gccagggcga tgaacccgac ccagaccgcg
      781 acgctggtgt tgtagcccag cagccagagc gaccagacgc cgccgacgat cccgaagggg
      841 agcgacgcca tcacgatcag cgactcggcc acgccccgga aattcaggta gagcagcagg
      901 aagatgagcg tcaacgtgac cggcaccacg atccacaggc gctgccggac ccgttgcatc
      961 gattcgaatt caccgctcca ctcgagccgg taccccaccg gcagcgtcag ccgttgctgc
     1021 accaggcgcc tggccgcgtt cacatagctg ccgacgtcgc tcccctcgat gtccactgcc
     1081 accgtggtga cggggaacgc gttctcggtc ttcacggcca tcggcccgct gacggtccgg
     1141 atgtcggcga tctggcccag cggcacctcc ccgccgccgg cggtggggac cacgatctgc
     1201 cggagcttgt ccacgttgtc ccggagctcc ggcggatacc ggacgttgat ctcgtagcgc
     1261 tcccggccct cgacggtcat cgcggcggcg tacccgcccg cggtcgcggt gatcgcatcg
     1321 tggacgtcgc cggcgttgag gccgtagcgc gccgcccggg cccggtcgac ccggatgtcg
     1381 acgtagttgc cggaattgtt ccgctcggcg aagacgttcc gggtgccggg cacctcccgc
     1441 acgatccgct cgaccgcttc gccgagctgc tgcaacgtgt cgaggtcggg cccgtagatc
     1501 ttgatgccca cgggcgtccg gatgccggtc gcgagcatgt cgatccggtt cttgatgggc
     1561 atcgtccatg cattggccac tcccggcagg cggacggccg aatccatttc gctcacgagc
     1621 cgcgggtagg tcatcccggc gcgccactcg gcggcgggct tgagcaccac catcgtctca
     1681 tacatgtcga gcggcgcggg atcggtggcg gtgttggcgc gtccggtctt ccccagcacc
     1741 gtggcgacct cggggaacga cttcagcacg ctgtcctggt agcgcatgat gccgatggcc
     1801 tgcccgatcg acacgccggg cacggtggtc ggcatgaaga gcagcgtccc ctcgttgagc
     1861 ggcggcatga actcactccc gagccgcagc cacggcagcg ccgtcacggc gacgatcgcg
     1921 accgcacccg ccaggacctt ccacggatgt gcgaggaccc accggatgac cggcgcgtag
     1981 atccggccga gccatcgatt gacgggattc gcgtcctggg accggatgcg cccgcggatg
     2041 aggtagccca tcagcacggg caccagcgtg atcgacagga gcgccgcgcc cgccatcgcg
     2101 aaggtcttgg tgaaggcgag cggcttgaag agccgcccct cctggagctc cagcgtgaac
     2161 accgggagga acgacacggt gatgatcagc agggagaaga acagcgccgg cccgacctgc
     2221 tcggcgctct cccgcaccac ctcccagcgg ggccgggcct cggcccccgg gcgttccagg
     2281 tgcttgtgca tgttctcgat catcacgatg gccgcgtcca ccatcgcgcc gatggcgatc
     2341 gcgatcccgc ccaggctcat gatattggcg ctcacgccta tcagtcgcat cacgatgaac
     2401 gagatcagga tgccgagcgg gagggtgacg atcgcgacca gggcggaccg gaagtgcagc
     2461 aggaagagga tggccacgac ggcaacgatg aggctctctt cgaggagctt gccccgcagg
     2521 ttgtcgacgg cgcggcggat gaggtccgac cggtcatagg ccggcacgag gcgcaccccc
     2581 ctgggcagcg tgcgctggat ctccgcgatc cgcgccttga ccgccttgat ggtccgccag
     2641 gcgtcggccc cgaagcgcat gaccacgatc ccggcgacgg cctcgccccg gccgtcgagg
     2701 tcggcggcgc cccgccgggg agcggggccg atgtgcacgt tcgccacgtc gcgcacccgc
     2761 acgggcgtgc ccgacggggt gctggacagc accacgtcgc cgatgtcggc ggtgctgcgc
     2821 aggtaaccga gcccccggat catgtactcc cggccggcca tttcgaggac ccgcgcgccg
     2881 atgtcggtgt tgtgggcgcc gatggcctcc accacccgca tcggtgacag gcggaacgcc
     2941 cggagccgct cggggtcgat gtccacctgg tactgcttga cgaacccgcc gaggctcgcg
     3001 acctcggaga cgccgggaat ggccgtcagc tggtagcgga tcgaccagtc ctgcaggctc
     3061 cggagctgcg ccaggtcgag cgagtcgctc tggagcgtgt attcgaagac ccagccgacg
     3121 ccggtcgcgt cggggccgag ggtcggttcg acgccctgcg gcaaccggct ccggatcccg
     3181 ttcaagtatt ccaggacccg cgagcgggcc caatagatgt cggtgccgtc ctcgaacagg
     3241 acatagacca gcgactgccc gaagaacgag aacccgcgca ccacccgcgt ccccgggact
     3301 ttgagcatct cggtcgcgat cgggtaggtg acctggtcct cgacgatctg gggcgcctgg
     3361 tcgctgaagt cggtctggac gatgacctgc acgtccgaga gatcggggat ggcgtccagc
     3421 ggagtggtca tgaccgccca caccccggcg gcagcgatcg ccgcggtgcc gaggagcacg
     3481 aggaagcggt tgcggagggc ccacgcgatg acggaccgga tcatggacgg acctccgcgg
     3541 ccggcggcgc gtccattccg ggcatcccgg ccatgctcgc gcccccggtg gtcaggtgcg
     3601 actcggcgtc caccaggaag ttggccgagg ccacgatccg ctgacccggg cggacgccgg
     3661 agaggacctg gatctggtcc ccggcccgaa ggccgagcgt gaccccctgc ggctcgagcg
     3721 tgccatccgt tcgcgcgatg tacaccatgt cccgctcgcc ggtccggacc accgcgtcgg
     3781 caggcacgtg ggggagccgc ggatcgagcg gcgcgaggag gaacagcgtg gcgtacatcc
     3841 cgggcttgag gagaccttcg cggttggcca ccgacacccg cacgaccgcg gtccggctca
     3901 tcgagtcgac cacgggcgag atgaacgtga tccggccggt ccacgtccgg ccgggatagg
     3961 atgcgaactc gacctgggcc cgggcccccg tatgcagcag cgccaggtcc tgctcgaaca
     4021 ccgccccatc gatccagacg gtcgagaggt cggcgaggcg gtacagcatg gcgccggtca
     4081 ccacggactg gccctcgacg accgccttgt ccagcaccac gccggacacc ggggacgtca
     4141 gcgtgagcgt cttggtgacc tccccggtgt gctcgaggcg ggccacctgc gcggccggga
     4201 tgtcggcgtt ggcgaggcgg cgccgcgtcg cggcgaggag ggacatcgca ctgctccggg
     4261 cccccggatc ggtcgagtcc gcgccggcca cgaggcccag cgccgtcagc agctcctgct
     4321 gcgcggcgac cagcgtcggg ctgtagatgc tgagcagcgg ctggccccgg cggaccggcg
     4381 cgccggtcgt cgcgacgaac atccgctcga tgtatccgtc caccttcacc gtgacatcga
     4441 ccacgctcga ttcgggcgcc gcgacctccc cgacggtccg gaccggccgc gtcagggcgc
     4501 ctgccaccac cgtcgtatag gtcacgccga tggccgatgc ctgcgccggc gtgaggcgca
     4561 cggcccccgg cgtcggcgcc gcggcctgca ccggcatctg catccccgcc atcgccggcg
     4621 gcggtgccgg cgtccgcgcg catcccgtcg ccacccacca ggcggcgacg ccgatcactc
     4681 cgatccgcgt gctcatggca aggctccggt cagcgtggcg aggtcggcct tggcctcctc
     4741 gtggtcggcg cgcaggcgcc aagtctcgat ctcgtagcgg gtgaccatca actggtcgtc
     4801 gaccagcgtc gcgaaatcga ccttccggac ctgataggcc gccagggccg actggaccgc
     4861 ggcgcgggcc tgcggcacga tccggtcctg gtacagccgc gcgagggtcc ggtcccgctc
     4921 cgccctggcc cgtgcctcga ccacttcggc cgtggtctcg ttccgcaggt cgagctgctc
     4981 ggcctgggcc atctgctgca tcgccgccat ctcgcgtcgc agcgggatct gccgtcgacc
     5041 gggaaagatc ggcagcgaca cgcccagcac caggctcacc atgtcgggga acgcgggccg
     5101 ctgctggtac gccaggccca cctggaagtc cggcaggcgc tcgcgctccg cggcgccaag
     5161 cgccgcatcg gccgcggcga tccgttcggc ccccgcctgg atcgccgccc ggccgccgag
     5221 cgcgagggcc gccagcgaat cggccgatgg gagc
//
LOCUS       DASUAP010000151         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_603962, whole genome shotgun sequence.
ACCESSION   DASUAP010000151 DASUAP010000000
VERSION     DASUAP010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603962"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFU45_03650"
     CDS             complement(<1..591)
                     /locus_tag="VFU45_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5358193.1"
                     /translation="MPAPVLSERDLSALRSFARRIDPADAGAHNNLGVLYYHKGLIPE
                     AVAAFQRALELDGRMAIAQENLEIAYRESGHYDRRVAELQGHLRTNPDDREVRWELGR
                     VYASLGHHDEAAAEFEALITRHPGDVAARIQLGLSERARGRVEEATEWFAEACRRDPE
                     SAVARFYYGETLYNRGLNEPALAALQDAVGRNPEYAD"
     gene            complement(600..1220)
                     /locus_tag="VFU45_03655"
     CDS             complement(600..1220)
                     /locus_tag="VFU45_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HEU5358194.1"
                     /translation="MEDVRALRDLQLDALREVANIGAGHAATALSQMTNRTIMISVPE
                     VNIRPLEEVADLLGAPDAVIAAVLMQMMGDLTGRTLILFPADAARRLCDILLRRSDGA
                     TTTFDEMEQSGLKEVGNILVSAYLNALSDFMGMMLVPSVPGLVVDQSAAVITSTYLNF
                     GQERDYVFCVETSFQVGKEGERLNGHFLLLPDMPSLRAIFDAIRLP"
     gene            complement(1233..>2350)
                     /locus_tag="VFU45_03660"
     CDS             complement(1233..>2350)
                     /locus_tag="VFU45_03660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEU5358195.1"
                     /translation="ELGRLDALMKHVGELVVSRNRLTDLASRRDDAELAGVAERIARL
                     VSEVQGEVLLARMSPVAEVFDRFPRVVRDLARELGKQARFESEGGEIELDRAVLDEIG
                     DPLLHLIRNAVDHGLEPPDERAARGKPAEGHVRVSAARERNTVVIRVADDGRGVDRAA
                     MLGRGKRDGLVDAGVEALTDDLLLKVLARPGFSTASAVSGVSGRGVGVDVALTRARAL
                     GGTLTVTSEAGVGTTFTLRVPLTLAIVRALLTGVGDERYAVPIAYVAETVDFDLRHVT
                     AVRDREALVVRDRAIPTVHLRRLVELDGEHPARRPGVILEVGERKAALIVDRLLGQQD
                     IVVEPFEAPRGMPTFFGGATILADGAPALILDAAALV"
BASE COUNT          346 a          877 c          784 g          343 t
ORIGIN      
        1 atcggcatac tcggggttgc ggccgacggc gtcctgcagg gccgcgagcg cgggctcgtt
       61 gagcccgcgg ttgtagaggg tttccccgta gtagaagcgg gccaccgcgc tctccgggtc
      121 gcgccggcag gcctcggcaa accactccgt ggcctcctcc acccggccgc gtgcgcgctc
      181 cgagaggccc agctggatcc gggcggccac gtcaccggga tggcgggtaa tgagggcctc
      241 gaactccgcc gccgcctcgt cgtggtgccc gagcgacgcg tagacccggc ccagttccca
      301 gcggacctcg cgatcgtcgg gattggtgcg caagtgcccc tgcagctcgg ccacccggcg
      361 gtcataatgg ccgctttccc ggtaggcgat ctccaggttc tcctgggcga tcgccatgcg
      421 cccgtcgagc tccagcgcgc gctggaacgc cgcaaccgct tccggaatga ggcccttgtg
      481 atagtagagg acgcccaggt tgttgtgggc gcccgcatcg gcaggatcaa tgcgccgggc
      541 gaacgaccgg agcgccgaga ggtcgcgttc ggacagcacg ggcgccggca tctcgcgtcc
      601 tacggcaggc ggatggcgtc aaagatggcg cggagcgagg gcatgtcggg cagcaggagg
      661 aagtgcccgt tcaggcgctc gccctccttc ccgacctgga aggaggtctc cacgcagaag
      721 acgtagtcgc gctcctggcc gaagttgagg taggtactgg tgatcaccgc ggcactctgg
      781 tccacgacca ggcccggcac cgacggcacc agcatcatcc ccatgaaatc gctgagcgcg
      841 ttgaggtacg cgctcaccag gatgttgccc acttccttga ggccgctctg ctccatctcg
      901 tcgaaggtgg tggtggcccc gtcgctgcgg cggagcagga tgtcgcagag ccggcgggcc
      961 gcgtccgccg ggaagagaat gagcgtgcgg ccggtgaggt cgcccatcat ctgcatgagc
     1021 acggcggcga tcacggcgtc cggcgccccg aggaggtccg ccacttcctc gagcggccgg
     1081 atgttgacct ccggcacgct gatcatgatg gtgcggttgg tcatctgcga gagggcggtg
     1141 gccgcgtgtc cggcgccgat gttggccacc tcgcgcagcg cgtccagctg caggtccctg
     1201 agcgcccgaa cgtcttccat gcagtcctcg cgtcagacga gcgcggcggc gtccaggatc
     1261 agcgccggcg ccccgtccgc cagaatggtg gcccccccaa agaacgtcgg catgccgcgc
     1321 ggcgcctcga agggttcgac aacgatgtcc tgctggccca gcagccggtc cacgatcagg
     1381 gccgccttcc gctccccgac ttccaggatc acgcccggcc gccgcgccgg gtgttcgccg
     1441 tccagttcca ccagccggcg caggtgcacg gtgggaatcg cccggtcgcg caccaccagc
     1501 gcttcgcggt cccgcaccgc ggtgacatgc cggagatcga aatccaccgt ctccgcgacg
     1561 taggcgatcg gcaccgcata ccgctcgtca ccaactccgg tgagcagcgc acgcacgatg
     1621 gccagcgtga gcggcacgcg cagcgtgaag gtcgtgccca cgcccgcttc ggacgtcacg
     1681 gtgagcgtcc cgcccagcgc ccgcgcccgg gtaagcgcca catccacacc cactccgcgt
     1741 cccgacaccc cgctgaccgc gcttgccgtg ctgaagccgg gccgggccag gaccttgagc
     1801 agcaggtcgt cggtgagcgc ctccaccccg gcgtcaacca gcccgtcccg ctttccccgc
     1861 cccagcatgg cggcgcggtc caccccgcgg ccgtcatcgg ccacccggat gaccacggtg
     1921 ttgcgctccc gggcggcgct gacccgcaca tgcccttccg ccggcttgcc gcgggccgcg
     1981 cgctcatcgg gtggttccag cccgtgatcc accgcattcc ggatcaggtg cagcagcgga
     2041 tcgccgatct cgtcgagcac ggcacggtcc agctcgatct cgccgccctc gctctcgaag
     2101 cgcgcctgct tgccgagctc ccgggcgaga tcccgcacca cgcggggaaa ccggtcgaac
     2161 acctccgcca ccgggctcat gcgcgccagg agcacctccc cctgcacctc cgacaccagc
     2221 cgcgcgatcc gttccgccac cccggcaagt tcggcgtcgt cgcgccggga ggccaggtcg
     2281 gtgagccggt tccgggagac caccagctcg cccacgtgct tcatgagcgc gtccaaccgc
     2341 ccgagctcga
//
LOCUS       DASUAP010000152         4898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1298437, whole genome shotgun sequence.
ACCESSION   DASUAP010000152 DASUAP010000000
VERSION     DASUAP010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298437"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1029
                     /gene="murQ"
                     /locus_tag="VFU45_03665"
     CDS             82..1029
                     /gene="murQ"
                     /locus_tag="VFU45_03665"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEU5358196.1"
                     /translation="MASDNPGSPTPIFLDRRLTERRNPRSRDIDVASSLELVDLINAE
                     DATVAPAVAAARLEIARAIDLIEAGFRAGGRLLYIGAGTSGRLGVLDASECPPTFGTP
                     PAMVVGIIAGGFPALVKPAEGAEDDASAGAGEMDARQVGASDVVVGIAASGTTPYVRG
                     GLGRARTLGARTVFVSCAEPPQAMVEGCDVCITVRTGPEVVTGSTRMKAGTATKLVLN
                     TLTTGAMIRLGKTYGNLMVDLEAWSEKLVDRGERILMEVAGLDRAAARAAIDAAGGSV
                     KTAIVMAGKGVDRDAARALLDAHAGRIRPILGPPPGIVE"
     gene            1026..2135
                     /locus_tag="VFU45_03670"
     CDS             1026..2135
                     /locus_tag="VFU45_03670"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEU5358197.1"
                     /translation="MTLRRALMVGLMSGTSLDGVDAALVRFDGPTRAELLGFVSRRYT
                     DEQREELVRALEGDATPQDLARLHVRLAEWAGEAVDRLLKQQHLKASDLAGIAFPGQT
                     LWHEPPTVSWQLGEPAVLVERFGVRVVSGFRAADVAAGGQGAPLVPMADVELFGAPDH
                     ARILLNLGGMANLTWVERLGREETAFAFDTGPGMAVVDAAARTVDPTLPFDRDGKVSA
                     LGKLDEELLKELLTHPYFSAAPPKSTGREHFGGEYAEALLERAPGPDAVATALELTAR
                     TVAEAIVRWVPPTPELVVSGGGAHHPRLLERIAALLPSGTVVRTFDELYFPGDAKEAV
                     AFALLGYLTLNGQPGNIPRATGARGPRVLGGITGP"
     gene            2132..3553
                     /locus_tag="VFU45_03675"
     CDS             2132..3553
                     /locus_tag="VFU45_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5358198.1"
                     /translation="MTRAGRLILPALRWQAATGFAHEREAIDRALEFGAGGFIIFGGT
                     ADAVRALTAELRTRAGRPLLFAADLERGAGQQFAGLTQLPPPAALASLGDPDTIGWAA
                     RTTAVEARSVGIQWILGPVADLDVEPRNPIVQTRAFGADPERVAADVVRWIEECTRGG
                     GLTCAKHWPGHSRTTADSHDGVPVVPASELEIQRELAPFRAAVAARVSAVMTAHVAFP
                     AIDPTGTPATFSSPILNKLRHGLGYNGAIVTDALMMGGATAAGPEDPAVRALAAGCDI
                     LLYPADPTASYRAIVAALERGDLTHARIEESVARYHRLLVSAPDVGTLPSIHPANDDH
                     ARKTALRTLIAPPEGAIRGRLTPPFELVVVDDDLGGRWPAGPTDGVEAELRTLDVPLA
                     PGGARIVLAFAEPRASKGRAGFGPESLARLADVTGTASLVILFGHPRLAAEIPGAAPV
                     LVAWHRERLMQEAAAWWLLERTR"
     gene            3550..>4898
                     /locus_tag="VFU45_03680"
     CDS             3550..>4898
                     /locus_tag="VFU45_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU5358199.1"
                     /translation="MKLLDWLVVLGYLIGALALGLALARRGTGSIAEFFVGGRSIPWW
                     LVATSMAATTFNVDTPLYVAGLVARYGVAGNWEWWSFAGAHVLLAVLLARLWRRVGVV
                     TDAELVELRYAGRTAAALRATRAFVFAVPINCIGIGYAMLAMRKVIIALGVADRLTFL
                     PGDSRLWAVLVIVLVTLAYTTVSGLWGVVATDFIQYLLAMFGAFVVMVYAVHEVGGLG
                     ALTAHFRLSGAGSRVNVLPTGSDALLPFTTFLGYIGIQWWAYRNSDGGGMFVQRLSAT
                     ATEREATKAAHLFNVLNYVVRTWPWVLVGLAALIILPGLADPEMAYPLLMVRYLPSGV
                     LGLVVASLIAAFMSSVSTQINWGASYIVNDLYGRFTGERDERKLLRAARIASVGLTLL
                     AATLSFFMESVGQVFRIMILIGTGTGAILLLRWFWWRINAWAEITALVSGLFLAAATV
                     "
BASE COUNT          600 a         1791 c         1764 g          743 t
ORIGIN      
        1 ggcgtccagt gggtgtcggg cgggcagggc gggggtccgg tggttgccgg ttccccgcgg
       61 cgggttctaa tttagcgcat cgtggcctcc gacaaccccg gctcccccac tccgatcttc
      121 ctcgaccgcc gcctgaccga acggcggaac ccgcgctcgc gcgacatcga tgtcgcgtcg
      181 agcctcgagc tcgtcgacct catcaacgcc gaggatgcga cggtggcccc ggcggtggcg
      241 gcggcgcgcc tggagattgc gcgcgccatc gacctcatcg aggcagggtt ccgcgccggc
      301 ggccggctgc tctacatcgg cgccggcacc agcgggcgcc tgggcgtgct cgacgcctcc
      361 gagtgtccgc cgaccttcgg caccccgccg gcgatggtgg tggggatcat cgccggcggc
      421 ttcccggcgc tggtcaagcc cgccgagggc gccgaggatg atgcgagcgc cggtgccggc
      481 gagatggacg cccgccaggt gggcgccagc gacgtggtcg tggggatcgc cgcgagcggg
      541 acgaccccct acgtccgcgg cgggctcggg cgcgcgcgga ccctcggcgc gcggacggtg
      601 ttcgtctcct gtgcggagcc gccccaggcg atggtcgaag gatgcgatgt ctgcatcacc
      661 gtacgcaccg ggcccgaggt cgtcaccggc tccacccgga tgaaggccgg caccgccacc
      721 aagctcgtcc tcaacacgct caccaccggg gcgatgatcc ggctggggaa gacctacggc
      781 aacctgatgg tggacctcga ggcgtggagc gagaagctgg tcgaccgggg ggagcggatc
      841 ctgatggagg tggcgggact cgaccgcgcg gcggcccgtg cggcgatcga cgcggccggc
      901 ggcagcgtca agaccgccat cgtgatggcc ggcaagggcg tcgaccgcga tgcggcccgg
      961 gcgctgctcg acgcgcacgc cggtcggatc cgtccgatcc tgggcccccc gccggggatc
     1021 gtcgaatgac gctccgccgc gcgttgatgg tcggcctgat gtcgggcacg tcgctcgacg
     1081 gcgtcgatgc ggcgctggtc cgcttcgacg gccccacccg cgccgagctg ctgggattcg
     1141 tgtcgcgccg gtacaccgac gagcagcgcg aggaactggt ccgtgccctc gagggggacg
     1201 ccacgccgca ggacctggcc cggctccacg tgcggctcgc ggaatgggcc ggcgaggcgg
     1261 tcgaccgcct gctcaagcag cagcacctga aggctagcga cctcgcgggg atcgccttcc
     1321 cggggcagac gctctggcac gagccgccta cggtgagctg gcagctgggc gagccggcgg
     1381 tcctggtcga gcgcttcggc gtccgggtgg tgagcggctt ccgcgccgcc gacgtggccg
     1441 ccgggggcca gggggcgccc ctcgtcccga tggccgacgt ggagctcttc ggcgcccccg
     1501 accacgcgcg gatcctcctc aatctcggcg ggatggccaa tctcacctgg gtcgaacggc
     1561 tcgggcgcga ggagaccgcc ttcgccttcg acaccgggcc ggggatggcc gtggtcgacg
     1621 cggcggcgcg cacggtcgac ccgacgctcc ccttcgaccg cgacggcaag gtgtcggcgc
     1681 tcgggaagct cgacgaggag ctcctcaagg agctgctgac gcacccctac ttttccgctg
     1741 cgccgcccaa gagcaccggc cgggagcact tcgggggcga gtacgcggag gcgctgctgg
     1801 agcgggcgcc ggggcccgac gccgtcgcga cggcgctcga gctcaccgcg cgcaccgtgg
     1861 ccgaggccat cgtccgctgg gtgccgccga cccctgagct cgtggtgtcg gggggcgggg
     1921 cgcatcaccc gcggctcctc gagcgcatcg cggcgctcct cccctcgggg accgtcgtcc
     1981 gcacgttcga cgagctctac tttcccgggg acgccaagga ggcggtcgcc tttgccctcc
     2041 tgggttacct caccctcaac ggccagcccg gcaacatccc ccgcgccacc ggcgcccgcg
     2101 gcccccgcgt cctcggcggg atcaccggcc cgtgacgcgc gccggtcgcc tgatcctgcc
     2161 ggcgctccgt tggcaggcgg cgacggggtt cgctcacgaa cgtgaggcca tcgaccgcgc
     2221 gctcgagttc ggggcgggcg gcttcatcat cttcggcggc accgccgatg cagtgcgtgc
     2281 actcaccgcc gagctccgga cccgcgccgg tcggccgctc ctcttcgccg ccgacctgga
     2341 gcgcggggcg ggtcagcagt tcgcaggcct cacgcagctc ccgcctcccg ccgcgctcgc
     2401 gtccctgggc gacccggaca ccatcgggtg ggcggcgagg acgaccgccg tcgaggcacg
     2461 gtcggtcggg atccagtgga tcctgggccc ggtcgccgat ctcgatgtgg agccgcggaa
     2521 cccgatcgtg cagacccggg ctttcggggc ggatcccgag cgcgtggccg ccgatgtggt
     2581 ccggtggatc gaggagtgca cccggggcgg cgggctgacg tgcgccaagc actggccggg
     2641 ccacagccgg accacggccg attcccacga cggcgtcccg gtcgtccccg cctccgagct
     2701 tgagatccag cgcgaattgg ccccgttccg tgccgccgtc gccgccaggg tatcggcggt
     2761 gatgacggcg cacgtggcgt tcccggccat cgacccgacc gggacaccgg ccaccttctc
     2821 atcgcccatc ctcaacaagc tgcgccacgg cctcgggtac aacggcgcca tcgtgaccga
     2881 cgcgctgatg atgggcggcg ccaccgcggc cggtccggaa gatcccgctg tccgtgccct
     2941 ggcggccggg tgcgacatcc tcctctatcc ggcagacccg accgcctcgt atcgcgccat
     3001 cgtggctgca ctcgaacgcg gcgatctcac ccatgccagg atcgaggagt cggtggcgcg
     3061 gtatcatcgg ctgctggtca gcgcccccga cgttggcacg ctcccgagca ttcaccccgc
     3121 gaacgacgac catgcccgaa agaccgccct ccgcacgctc attgcccccc ctgagggcgc
     3181 catccgcggg cgcctgaccc cgccattcga gctggtggtg gtcgatgacg atctgggcgg
     3241 ccgctggccg gccggtccca ccgatggggt cgaagcggag ctccggaccc tcgacgttcc
     3301 gttggccccg ggtggcgctc gcatcgtcct cgcctttgcc gagccgcggg catcgaaggg
     3361 gagggcggga ttcgggccgg agagcctcgc acggctcgcc gacgtgaccg ggacggcgtc
     3421 gctggtgatc ctcttcggcc atccgcggct cgcggccgag atccccggcg cggcgccggt
     3481 cctggtcgcg tggcaccggg agcggctgat gcaggaggcc gccgcctggt ggctcctgga
     3541 gcgcacgcgg tgaagctcct cgactggctg gtggtgctgg gctacctcat cggtgcgctg
     3601 gccctcgggc tcgcgctcgc ccgtcggggc accgggagca tcgccgagtt cttcgtcggc
     3661 ggacggtcga tcccctggtg gctcgtggcc acctcgatgg ccgcgacgac gttcaacgtg
     3721 gacacgccgc tctacgtggc gggcctcgtg gcgcgatacg gcgtggccgg caactgggag
     3781 tggtggagct ttgccggagc gcacgtgctc ctcgcggtgc tgctggcccg gctctggcgc
     3841 cgggtgggcg tggtgaccga cgcggaactc gtggagctcc gctacgccgg ccggaccgcc
     3901 gccgcgctcc gggccacccg cgcgttcgtc ttcgcggtgc ccatcaactg catcggcatc
     3961 ggctatgcga tgctcgcgat gcggaaggtc atcatcgccc tcggcgtcgc cgaccgcctg
     4021 accttcctgc ccggcgactc ccggctctgg gccgtgctcg tcatcgtgct cgtcaccctg
     4081 gcctatacca ccgtgtcggg gctctggggt gtcgtggcca ccgacttcat ccagtacctg
     4141 ctggcgatgt tcggcgcctt cgtggtgatg gtctacgcgg tccacgaggt cggaggcctg
     4201 ggcgccctca cggcgcactt ccggctcagc ggggcgggga gccgggtgaa cgtgctgccc
     4261 accggaagcg atgcactgct gccgttcacc acgttcctcg gctacatcgg catccagtgg
     4321 tgggcctacc ggaacagcga cggcggcggg atgttcgtcc agcggctctc cgccaccgcc
     4381 accgagcggg aggcgacgaa ggccgcgcac ctcttcaacg tcctcaacta cgtggtgcgc
     4441 acctggccct gggtgctggt ggggctcgcg gcgctcatca tcctgcccgg gctcgccgat
     4501 ccggagatgg cctatcccct gctgatggtg cgctacctcc cgtccggcgt cctcggcctc
     4561 gtggtcgcga gcctcatcgc cgcgttcatg tcgagcgtct ccacccagat caactggggc
     4621 gccagctata tcgtgaacga cctctacggc cgcttcaccg gcgagcgcga cgagcggaag
     4681 ctcctccgcg cggcgcggat cgcgtcggtg gggctcacgc tcctggccgc gacgctctcc
     4741 ttcttcatgg agagcgtcgg gcaggtcttc cggatcatga tcctgatagg gacgggcacc
     4801 ggcgccatcc tcctgctccg gtggttctgg tggcggatca acgcctgggc ggagatcacg
     4861 gcgctggtct cggggctctt cctcgccgcg gcgacggt
//
LOCUS       DASUAP010000153        12572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_985533, whole genome shotgun sequence.
ACCESSION   DASUAP010000153 DASUAP010000000
VERSION     DASUAP010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 12572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12572
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985533"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFU45_03685"
     CDS             complement(<1..350)
                     /locus_tag="VFU45_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU5358200.1"
                     /translation="MTDPVLAAADAIRARLAGRKPTIAIVLGSGLGHLAERVADAVRI
                     PYDAIPGFHVPTVVGHKGELVIGTLGGRQVIAQSGRFHAYEGHSAEVTALPVRVFAAL
                     GVRTLLLTNAAGGIR"
     gene            complement(541..1983)
                     /locus_tag="VFU45_03690"
     CDS             complement(541..1983)
                     /locus_tag="VFU45_03690"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEU5358201.1"
                     /translation="MSPIRRGLSSAGGRFLFMCVLLTLCLGAAWIVPGLTGHAVGGLL
                     AQQPAAATPAPAAPQVAAPVQYSDQSTHTPWTTRLNGLIGLVLIVGVGILFSSKRKAI
                     RWRIIAWGLGLQFAFAVFVLRIPFGQHLFRTLGDFVTKVLSYSYAGSQFVFGDLGKQG
                     SSFGVIFAFQVLPAIIFVSALFAIMYYLGVMQVVVRAFAAVMSKVMGASGAESLNVAA
                     SIFMGQTEAPLTIRPFLPTMTQSELMTVMTSGMAHISGSIMAAYIAFGVEARHLLTAV
                     IMTAPGTIMMAKLFEPETEVPETMGKLQHDIPRTDVNVVDAAARGTGEGLHLMLNVIA
                     MLISFLALVALTNGAFGWVHGLLPWFPQDLQTVFGWVGRPVAWAMGVPWKDSGVIGGL
                     LGTRAVLNEFIAFSQLGPLKATLDPRSFVIASFALAGFANISSVGIQIGGIGALAPNR
                     KGDLAKLGFRAMLAGTLANFLSATIAGMLL"
     gene            complement(2037..3059)
                     /gene="add"
                     /locus_tag="VFU45_03695"
     CDS             complement(2037..3059)
                     /gene="add"
                     /locus_tag="VFU45_03695"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU5358202.1"
                     /translation="MLSRDLLRRLPKAELHVHLDGSLRPGTMIDLARTARVELPARDP
                     DALRRYMLVDDARDLEDYLKRFDVTIALLQDPAAIERVAYEMVEDARADGLRYLEVRY
                     CPVLSTRLGLTIEDAVAAEWRGLQRGQREFGVRTAIINCSLRHYAPEVSADLARRSVA
                     LRHQGVVAFDLAGGEAGRPAAVHREAFDIAEQGGLGITVHAGEAAGAESIAQAVYQCH
                     ATRIGHGTRLYEDPALKARLRDRRVLLETNITSNVQTHAVASAAVHPVRGYLADGLAA
                     TLCTDSWLMSGTSLTDEYWLAHTALGMGRAEIDRMILHGFDGAFLPWPEKQALLADVR
                     EELRSL"
     gene            complement(3046..3297)
                     /locus_tag="VFU45_03700"
     CDS             complement(3046..3297)
                     /locus_tag="VFU45_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358203.1"
                     /translation="MAADLRCFVNDRGVTVPSGASALDAVRAFDAAMADRIAAGEGHL
                     TDARGLPLPLEAPVGTGDIIRAVISARRGGRTAEFDALP"
     gene            complement(3297..6320)
                     /locus_tag="VFU45_03705"
     CDS             complement(3297..6320)
                     /locus_tag="VFU45_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5358204.1"
                     /translation="MPNRRAALRGAALSVALAASLAFPLSAQDTAHVVIVATTDVHGH
                     ATGWDFVDGRPFPGGLTRVGSVVDSLRRRYPGQVVLVDAGDLIEGDPFAAYFATVAPQ
                     DPDPMVDALNGLAYDAAVPGNHEFNFGVPFFERAMSGARYAVVAANIVAASGKPPFPP
                     YTVVQRGGVKVAIGGLTTPGSMVWDREHLRGQVRIDPIASSAPALLGELRAEGDVAVV
                     LVHSGMDGESSYDTTGVGPENAAASLASGDVRPDVVVVGHTHREMVDSVINGVHFVQP
                     RVYAQSVAVVHLSLARRGGHWAVTAIHADRVPLDREPESSALVRRLAPARSAVTGWIS
                     QPIGYAVGPFPAQGARASATPLMGLVGEAERRAAHADLAAVSAFSTSGGLPAGEIHRS
                     DIFSVYPYENTLGAVRISGAQLRAYLEQAARYFRVGPGGRVTLNDSVPGYNYDMVWGA
                     DYLIDLSKPEGQRITSLSVKGRPVAPTDSFTLAVNSYRLSGGGGYTMLAGAPVTYDRG
                     GSVRDLLEAAVGRDTLHPRDYARENWRIVPDRAAEEVRALFGLKPLARAPVPTDTVEL
                     RILALGALHGQLDPDGANGGVAALKTVLDSVAARCDCPVLKVAAGELLQGTRASDLVH
                     GQSMVEALDRLGLDATAVGVGDLAWSADTLERRMSGSRFAWVTANYLDPATRQRPDWA
                     IPYRVLAIGKRKVAVVGFTAGQDSLAPGLAALRDAAGRARAERPDLLVLLASGEAAAI
                     AQGLPPATFDAIVSASGPDTTVNGTPILAGASRGVAVSRVDLVRTVVGGRIVRRGVDT
                     VRADRVRADSGEVALLARYGGAVDSAASRVVATAKLPLLRSPAQSPLGNLVVDAWRNA
                     LRTDIAILADSELHSDLPAGPLTYGTLRDLVGAYGTLERRTWTGAGLRQFLEEALAGD
                     GPPSFHVSGLTITYDPKGRPGRRVKDIRLVDGSKLRDRTRYWVAASAAVPAVPGQVVA
                     PDDAPVRALHAVITYFSALPQPIEAPERPRFVAAR"
     gene            6408..6773
                     /locus_tag="VFU45_03710"
     CDS             6408..6773
                     /locus_tag="VFU45_03710"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5358205.1"
                     /translation="MPRPSSTDLLVSIAEPTRLRILNCLAAAPLFVSDLQAILRLPQP
                     TVSRHLRILRDAGVVRDTPIAQFVLYRLRRGADADARLLAAVLESLGQDESLRIERQR
                     AADRSHTHTRLRTVEPVSE"
     gene            6770..7492
                     /locus_tag="VFU45_03715"
     CDS             6770..7492
                     /locus_tag="VFU45_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5358206.1"
                     /translation="MIKPGTPAHRILVVDDEPDITALVAYHLAKAGHRVSTAANGPDA
                     LRAAREERPDLVILDVMLPGTSGYDVLAELRRREETREVGVILLTARREEPDRIRGLE
                     LGADDYLTKPFSPQELVLRVDAVLRRLTAPAITGGSTLTAGPVVIDRSAHKVLVDGEE
                     VLLTALEYKLLLTLVERRGRVQDRPQLLETVWGHQPDIQTRTVDMHVQRLRTKLGAAG
                     DLIETVRGAGYRFQGAERGRGK"
     gene            7489..9225
                     /locus_tag="VFU45_03720"
     CDS             7489..9225
                     /locus_tag="VFU45_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5358207.1"
                     /translation="MNLSGRVVAGSIALLAGAMTIVVGASEVSLRRDMEASLARALEA
                     EARIVASALPADSTQWGRAVRQLAGSGGHRITLIDTLGRVRADNEVSDATLPFVENHR
                     MRPEVQTALARAEGEAVRHSVTVGEDLLYVAVRGGPGIVRVSAPYAPVQDTLDRARRA
                     VLWAALLALLVGSVAALIATRSITAPLRRITVAARAIARGRPPVLPITTIPDVNALVV
                     ALREMDEELSARFEALGREQARIAAVVESMLEGVIAADPRGTVVLANSAARRFLGYPA
                     GAALPVFDELFRTKAAREVVRDVRAGESVTGREVELEGRTLLATARPLPQGGAVLVLH
                     DLTEVRRLQVMRRDFVANVSHELKTPLTSISGYAETLISDRPPPDVAERFLQTILGNA
                     RRMQRLVDGLLDLSRVESGHWRPTPERVDLVAIVREAWDGLGDRADGRGTELTVDVAA
                     DARAIVTDADALRQVFTNLLENARRYSPPGAPVTVSARREGRGTRIAVGDRGPGIGAE
                     HLPRIFERFYRADPSRSRDEGGTGLGLAIVKHLVEAQGGRVWAESRVGAGTTVNAWLP
                     DVVDGGAGAAVS"
     gene            9371..10396
                     /locus_tag="VFU45_03725"
     CDS             9371..10396
                     /locus_tag="VFU45_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEU5358208.1"
                     /translation="MRLVARRTIALLAVFAAIGAGRAAAQQTQPQVSVGGVVLAQYAY
                     QLKDPADSNAFDVTRAYLNVTGKFSGGLMTRVTTDVYRAADSSTVIRLKYAYLAWTPE
                     KSPLTYRFGLTQTPWIDYEETLYDYRMQGPTVLDRNGYITPSDFGAAVDGSWNQDKFN
                     FQAGVYNGEGYSKGSTGDQRKDVSARASLRLMDTDDNSRVGGLRLTAFGEAGKPTGGG
                     TRNRISGQVSYKTKQLTLAGEYTAASDSVAASTSTIKGSILSAFGVYKVPASKVALVA
                     RLDVVDPNTNATGDKQTRIIAGVSYQVTPNWRAMVDLDNLSYESTPTAAQEAKRSTLY
                     FHTQLTF"
     gene            10424..11476
                     /gene="pstS"
                     /locus_tag="VFU45_03730"
     CDS             10424..11476
                     /gene="pstS"
                     /locus_tag="VFU45_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU5358209.1"
                     /translation="MASIASLRARLGLLGLAAAAGAAPLAAQSLTGAGSTFGTPIYTA
                     WAAALDQKTGIQVNYQSIGSGGGIQQFTQGTVDFGATDGPMTDAQIVAAQGKVLHIPT
                     VLGAVVLTWNLPALGDRKLKLDGPTIAGIYSGEITRWNDRRIAELNPEVKLPAQDIIV
                     VHRSDGSGTTYIFTDYLSKVSPEWKQKVGFATSVNWPLGLGGKGSEGVTQQVKQTEGA
                     IGYVELIYAVTNHLPFAYVKNAAGTFVDPSLQTTTNAAASARWSKDTDFRVSITNAPG
                     KEAYPISSFTWLIIRPDVADPVKAKALKTFFEWMITPEAQAMAQKLQYAPLPAEVVKL
                     IRARLNTLTSGGKPIR"
     gene            11708..>12572
                     /gene="pstC"
                     /locus_tag="VFU45_03735"
     CDS             11708..>12572
                     /gene="pstC"
                     /locus_tag="VFU45_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU5358210.1"
                     /translation="MTHAASTPSGLEERHSGDRIFRIVLTAAAATIPILLAALVWELW
                     VGALPAIHRFGASFLVRDVWDPVAGDFGAFPLIVGTLVSSFLALVIAVPLALGVAIFL
                     TEFAPRWIRQPISFLVELLAAVPSVVYGLWGIFVLVPFLRTTVFPFLRQALGFLPFFQ
                     GPIYGPSMLAAAVILAIMILPFIMSVAREVLMAVPGAQREAALALGATRWEAVATAVV
                     PYARSGIVGAAILGLGRALGETMAVTMLIGNTHDISASLFAPGYTMAAVIANEFSEAA
                     TPLHFAALTYVA"
BASE COUNT         1853 a         4477 c         4526 g         1716 t
ORIGIN      
        1 cggatcccgc ccgcggcgtt ggtaaggagc agcgtgcgga cgccgagcgc cgcgaagacc
       61 cggaccggca gcgccgtgac ctcggccgag tggccttcgt aggcgtggaa gcggccgctc
      121 tgcgcgatga cctgcctgcc cccgagcgtc ccgatgacga gctccccctt gtggcccacg
      181 accgtgggga cgtggaagcc ggggatcgcg tcgtagggga tccgcaccgc gtcggcgacc
      241 cgctcggcga ggtggccgag gccggagccg aggacgatgg cgatcgtcgg cttgcggccg
      301 gcgagcctgg cgcggatcgc gtccgcggcg gcgagaacag gatcggtcac ggatcctccg
      361 gcattgataa gcgttcgcac atcacaaaga aggcgtggga ccacggagga cacggagggg
      421 cacggagaac ggcatatgtc gtggtctcac cggaggagct ccgtccgcgt caccactttg
      481 gatcgctctt ctccgtgcct ctccgagtcc tccgtgtcct ccgtggtccc acgtcctgga
      541 tcagagcagc atccccgcga tggtggcaga gaggaagttg gcgagcgtgc cggcgagcat
      601 ggcgcggaag ccgagcttgg ccaggtcgcc cttgcggttg ggggccaggg cgccgatgcc
      661 gccgatctgg atgcccaccg agctgatgtt ggcgaagccc gccagggcga agctcgcgat
      721 gacgaacgac cggggatcga gcgtggcctt gaggggcccg agctgggaga aggcgatgaa
      781 ctcgttcagc accgcgcggg tgccgaggag cccgccgatg acgccgctgt ccttccacgg
      841 cacgcccatg gcccaggcca ccggccggcc gacccagccg aagaccgtct ggaggtcctg
      901 ggggaaccag ggcaggaggc cgtggaccca gccgaaggcg ccgttggtga gcgccaccag
      961 ggccaggaag ctgatcagca tcgcgatgac gttgagcatc aggtggagcc cttcgccggt
     1021 gccgcgcgcc gccgcatcga ccacgttgac gtcggtgcgc gggatgtcgt gctgcagctt
     1081 ccccatcgtc tccggcacct cggtctccgg ctcgaagagc ttggccatca tgatggtgcc
     1141 cggcgcggtc atgatgacgg cggtgaggag gtgccgcgct tcgacaccga aggcgatgta
     1201 cgccgccatg atcgacccgg agatgtgcgc catcccggag gtcatcacgg tcatgagctc
     1261 ggactgcgtc atcgtgggga ggaagggccg gatggtcagc ggcgcctcgg tctgccccat
     1321 gaagatcgac gccgcgacgt tgaggctctc cgcgccgctc gcccccatca ccttgctcat
     1381 caccgccgca aaggcccgca ccaccacctg catcacgccg aggtagtaca tgatggcgaa
     1441 gagcgcggac acgaagatga tggcgggcag gacctggaag gcgaagatga cgccgaagct
     1501 cgacccctgc ttgccgaggt cgccgaagac gaactgggac ccggcgtacg agtagctgag
     1561 gaccttggtg acgaagtcgc ccagggtccg gaagaggtgc tggccgaagg ggatgcggag
     1621 cacgaagacc gcgaaggcga actggagccc gagcccccac gcgatgatcc gccagcggat
     1681 ggccttccgc ttcgacgaga acaggatccc gacccccacg atgagcacca gcccgatcag
     1741 gccgttgagc cgcgtggtcc acggggtgtg ggtggactgg tcggagtact gcaccggggc
     1801 cgccacctgg ggcgccgccg gggccggcgt cgccgcggcc ggctgctggg ccagaagccc
     1861 gcccacggcg tggccggtga gccctggaac gatccacgcg gcgccgaggc agagcgtcag
     1921 gaggacgcac atgaagagga agcggccacc ggcggacgac aggcctcgac ggatcgggga
     1981 catcaggttc ctcgggcgtt gggtggggag cacgcggccg tccggggccg cgccggtcag
     2041 agcgaacgca gttcctcgcg cacgtcggcc aggagcgcct gcttctcggg ccacgggagg
     2101 aaggcgccgt cgaatccgtg gaggatcatc cggtcgatct cggcgcgtcc catgccgagc
     2161 gccgtgtgcg ccagccagta ttcgtcggtg agcgacgtgc cgctcatgag ccagctgtcg
     2221 gtgcagagcg tggcggccag gccgtcggcc aggtagccgc ggaccgggtg aacggcggcg
     2281 ctcgcgaccg cgtgggtctg gacgttgctc gtgatgttgg tctcgagcag tacgcgtcga
     2341 tcgcggagcc gggccttgag cgccgggtcc tcgtacaggc gggtgccgtg gccgatccgg
     2401 gtggcgtggc actggtacac cgcctgggcg atcgactcgg cgccggccgc ctcgccggcg
     2461 tggacggtga tcccgagccc cccctgctcc gcgatgtcga acgcctcgcg gtggaccgcg
     2521 gccggtcgcc ccgcctcccc gcccgccagg tcgaaggcga ccacgccctg gtgccggagc
     2581 gccaccgaac gtcgcgccag gtcggcggag acctccggag catagtgccg gagggagcag
     2641 ttgatgatgg cggtccgcac gccgaattcc cgctgcccac gctgcaggcc ccgccactcg
     2701 gcggcgaccg cgtcctcgat ggtgagcccg agccgggtgc tcagcacggg gcagtagcgc
     2761 acttcgaggt agcggagccc gtcggcccgg gcgtcctcga ccatctcgta ggccacccgc
     2821 tcgatggcgg cggggtcctg cagcagcgcg atggtgacgt cgaaccgctt gaggtaatcc
     2881 tcgaggtcgc gggcgtcgtc gaccagcatg tagcggcgga gcgcgtcggg atcgcgggcc
     2941 gggagctcga ccctggccgt gcgggccagg tcgatcatcg tcccggggcg aagcgaaccg
     3001 tcgaggtgga cgtgcagctc cgccttcggg agccgccgca ggaggtcacg ggagagcatc
     3061 gaactccgcg gtgcggccgc cccggcgggc ggagatgacg gcgcggatga tgtccccggt
     3121 accgaccggc gcctcgagcg gcagcggcag gccccgtgcg tcggtgaggt gcccctcgcc
     3181 cgcggcgatc cggtcggcca tcgcggcgtc gaaggcccgc actgcgtcga gcgccgaggc
     3241 accggacggg acggtgacgc cgcggtcgtt cacgaagcag cggaggtcgg cggccatcag
     3301 cgggccgcca cgaagcgggg gcgttccggc gcctcgatcg gctgcggcag cgcgctgaag
     3361 taggtgatca ccgcgtggag cgcccgcacc ggggcgtcgt ccggcgccac cacctggccg
     3421 ggcaccgccg gcaccgcggc cgacgccgcc acccagtacc gcgtccggtc acgaagcttc
     3481 gacccgtcga cgagccggat gtccttgacc cgccgccccg gccggccctt gggatcgtag
     3541 gtgatcgtga gcccggacac gtggaaggag ggcgggccgt cgccggcgag tgcttcttcg
     3601 aggaactgcc ggagtcccgc cccggtccag gtgcggcgtt ccagcgtgcc gtaggcaccc
     3661 accaggtcgc gcagggtgcc gtacgtgagc ggcccggcgg ggaggtcgga gtggagctcg
     3721 ctgtccgcca ggatggcgat gtcagtgcgg agcgcgttgc gccaggcgtc gaccaccagg
     3781 ttcccgagcg ggctctgcgc cggcgaacgc aggagcggga gcttggcggt ggctacgacg
     3841 cgcgacgcag cggaatcgac cgcgccgccg tagcgcgcca gcagcgcgac ctcgccggaa
     3901 tcggcccgga cccggtcggc gcggaccgtg tcgacgccgc gccggacgat gcggccgccg
     3961 accacggtcc ggaccaggtc cacccgcgag accgcgactc cgcgagacgc gccggccagg
     4021 atcggggtac cgttcaccgt cgtgtccggc ccagatgccg agacgatcgc gtcaaaggtg
     4081 gcaggcggga gcccctgcgc gatcgccgcg gcctcgccgc tcgccaggag caccaggagg
     4141 tccggacgct cggcccgggc gcggcccgcc gcgtcgcgaa gggcggcaag ccccggtgcg
     4201 agcgagtcct ggccggcggt gaagcccacc accgcgacct tccgctttcc gatggcgagc
     4261 acgcggtacg ggatggccca gtccgggcgc tgccgtgtcg ccgggtcgag gtaattcgcc
     4321 gtgacccacg cgaagcggga gccggacatc cggcgctcga gggtgtccgc cgaccacgcc
     4381 aggtcgccca cgccgaccgc cgtcgcatcg agacccagcc ggtcgagcgc ctcgaccatc
     4441 gactgcccgt gcaccaggtc ggacgcgcgc gtcccctgca ggagctcacc ggccgcgacc
     4501 ttcaggaccg ggcagtcgca ccgcgcggcg acgctgtcga ggacggtctt gagcgcggcg
     4561 acgccgccat tggcgccgtc gggatcgagc tgcccgtgga gcgcgccgag cgccaggatg
     4621 cggagctcca cggtgtcggt cggcaccggc gcccgcgcca gcggcttgag tccgaagagc
     4681 gcgcgcacct cctcggccgc ccggtcggga acgatgcgcc agttctcccg ggcatagtcc
     4741 cgcgggtgca gggtgtcccg gcccaccgcg gcctcgagga ggtcgcgcac cgacccaccc
     4801 cggtcgtagg tgaccggcgc gccggcgagc atcgtatagc cgccaccgcc ggacaggcgg
     4861 tagctgttga ccgcgagggt gaagctgtcg gtcggcgcca ccgggcggcc cttcaccgac
     4921 aggctcgtga tccgctgccc ctcgggtttc gacaggtcaa tcaggtagtc ggcgccccac
     4981 accatgtcgt agttgtaccc cgggacgctg tcgttgagcg tcacgcggcc gcccgggccc
     5041 acccggaagt aacgggcggc ctgctcgagg taggcgcgca gctgggcacc cgagatccgc
     5101 acggcgccga gggtgttctc gtacgggtag accgagaaga tgtcggatcg gtggatctca
     5161 ccggcaggca ggccgccgct ggtgctgaag gcggagaccg cggcgaggtc ggcgtgggcg
     5221 gcgcggcgtt cggcctcccc cacgagtccc atcagcggcg tcgccgaggc ccgggcgccc
     5281 tgcgccggaa agggcccgac cgcatagccg atcggctggg agatccaccc ggtcacggcg
     5341 ctccgggcgg gcgcaagccg gcggacgagc gccgagctct ccggctcgcg gtcgagcggc
     5401 acccggtcgg cgtgaatggc ggtgacggcc cagtggcccc cgcggcgggc gagcgagagg
     5461 tgcaccaccg ccacgctctg cgcgtagacc ctgggctgga cgaaatggac gccattgatg
     5521 acggagtcga ccatctcgcg atgggtgtgc ccgacgacca cgacgtccgg ccgcacgtcg
     5581 cccgaggcga ggcttgccgc ggcgttctcc ggtccgacgc cggtcgtgtc gtacgacgat
     5641 tccccgtcca tcccgctgtg caccagcacg acggcgacgt ctccctcggc gcgaagctcg
     5701 ccgagcaggg cgggcgccga cgaggcaatg ggatcgatcc gcacctggcc ccgcaggtgc
     5761 tcccgatccc acaccatcga tcccggtgtg gtcagtccgc cgatcgcgac cttgaccccg
     5821 ccccgctgca ccaccgtgta gggcgggaac gggggcttgc ccgaagccgc gacgatattg
     5881 gcggcgacga cggcgtagcg ggcgccgctc atcgcccgct cgaagaacgg aacaccgaag
     5941 ttgaactcgt ggttgcccgg gaccgccgca tcgtacgcca ggccgttgag cgcatcgacc
     6001 atcgggtcgg gatcctgcgg ggccacggtc gcgaagtagg ccgcgaacgg gtcaccctcg
     6061 atgaggtcgc ccgcgtcgac caggaccacc tggccggggt accggcggcg cagcgagtcg
     6121 accaccgacc ccacgcgggt gagcccgccc gggaacggcc ggccatcgac gaaatcccac
     6181 ccggtggcgt gaccgtgcac gtcggtcgtc gcgacgatca cgacgtgggc ggtgtcctgg
     6241 gcggagagcg ggaaggcgag ggacgcggcg agcgctacgg agagagcggc gccacggagg
     6301 gccgcacggc ggttcggcat gaaggaatat ccgccggccc cgcaccgcgg gcaacggcgc
     6361 cggcttgcgt ccattcgccc gccggtatat acgttcaagc atatatggtg ccgcgacctt
     6421 cgtccaccga cctgctggtc tcgatcgccg agccgacccg gctccggatc ctcaactgcc
     6481 tggcggcggc tccgctgttc gtttccgacc tgcaggcgat cctgcgcctt ccccagccga
     6541 cggtgtcgcg ccacctccgg atcctgcgcg atgccggcgt ggtgcgggac acgccgatcg
     6601 cccaattcgt gctctaccgg ctccgccggg gcgccgacgc cgatgcgcgc ctcctcgccg
     6661 cggtcctcga gagcctgggc caggacgaga gcctccgcat cgaacggcag cgcgcggcgg
     6721 accgcagcca cacgcacacc cggctccgga cggtggagcc cgtctccgaa tgatcaagcc
     6781 cggcacgccc gcgcaccgca tcctcgtcgt cgatgacgag cccgacatca ccgcgctcgt
     6841 tgcgtatcac ctggccaagg cgggccatcg cgtctccacg gcggccaacg gccccgacgc
     6901 gctccgcgcc gcccgggagg agcgccccga cctggtgatc ctcgacgtga tgctccccgg
     6961 cacctcgggg tacgacgtgc tggccgagct ccgccggcgg gaggagacgc gggaggtcgg
     7021 ggtcatcctc ctcaccgcgc gccgggaaga gcccgaccgg atccggggcc tggagctcgg
     7081 cgccgacgac tacctgacca agcccttctc gccgcaggaa ctggtgctca gggtcgacgc
     7141 cgtgctccgg cgcctgaccg cccctgccat cacgggcggc tccaccctca ccgcaggccc
     7201 ggtggtcatc gaccgttcgg cgcacaaggt gctggtcgac ggcgaggaag tgctcctcac
     7261 cgctcttgag tacaagctgc tgctgacgct ggtcgagcgg cggggccggg tgcaggaccg
     7321 gccgcaactc ctggagacgg tctggggcca ccagcccgac atccagaccc gcaccgtcga
     7381 catgcacgtg cagcggctgc gcaccaagct gggcgccgca ggcgacctca tcgagaccgt
     7441 ccgcggcgcc ggctaccgct tccagggcgc cgagcggggg cgtggcaagt gaacctgtcg
     7501 ggccgggtgg tggccgggtc gatcgcgctg ctcgcgggtg cgatgaccat cgtcgtgggc
     7561 gcctcggagg tctcgctccg gcgcgacatg gaggcgtcgc tcgcgcgcgc gctcgaggcg
     7621 gaggcgcgga tcgtggcctc cgcgctcccg gccgactcga cccagtgggg ccgtgcggtg
     7681 cggcagttgg ccggctcggg cgggcaccgc atcaccctca tcgacaccct tggccgggtc
     7741 cgcgcggaca acgaggtctc cgacgccacg ctcccgttcg tcgagaacca ccggatgcgg
     7801 ccggaggtcc agacggcgct ggcccgcgcc gagggtgagg cggtgcgcca cagcgtcacc
     7861 gtgggcgagg acctgctcta cgtcgccgtt cggggcggcc ccgggatcgt gcgggtctcg
     7921 gcgccgtacg cgccggtgca ggacaccctc gaccgcgccc gccgggccgt gctctgggcc
     7981 gcgctgctgg cgctcctcgt cgggagcgtg gcggccctca tcgccacccg gtcgatcacc
     8041 gccccgctcc gccggatcac cgtcgccgcg cgggccatcg cccgcgggcg gccgccggtc
     8101 ctgccgatca ccaccatccc cgacgtgaac gcactggtgg tggcgctccg ggagatggac
     8161 gaggagctct cggcgcggtt cgaggcgctc ggccgggagc aggcccgcat cgcggccgtg
     8221 gtggagtcga tgctcgaggg ggtgatcgcc gccgacccgc gggggaccgt cgtgctcgcc
     8281 aactccgccg cgcgccggtt cctcggctat ccggccggcg ctgcgctccc ggtgttcgac
     8341 gagctcttcc ggaccaaggc ggcgcgcgaa gtggtgcgcg acgtccgggc cggggagtcc
     8401 gtgaccgggc gcgaggtgga gctcgagggg cggaccctcc tcgccaccgc gcggccgctc
     8461 ccccagggcg gcgccgtgct cgtcctgcac gacctgaccg aggtgcggcg cctgcaggtg
     8521 atgcggcgcg acttcgtggc gaacgtttcg cacgagctca agacgccgct cacctccatc
     8581 tcgggctacg ccgagacgct catcagcgac cgcccgcccc ccgacgtggc ggaaaggttc
     8641 ctccagacca tcctcggcaa tgcgcgcagg atgcagcgac tggtggacgg cctgctcgac
     8701 ctgtcacggg tggagtcggg gcactggcga ccgacgccgg agcgggtcga cctcgttgcc
     8761 atcgtgcgcg aggcgtggga cggcctgggc gatcgcgcgg acggccgagg gaccgagctc
     8821 acggtggacg tcgcggcgga tgcgcgggcg atcgtgaccg acgccgacgc gctccgccag
     8881 gtgttcacga acttgctgga gaacgcgcgc cgctactcgc ccccgggggc gccggtgacc
     8941 gtgtccgccc ggcgggaggg gcgcgggacg cgcatcgccg tgggcgaccg gggcccgggg
     9001 atcggggcgg agcacctgcc gcggatcttc gaacggttct atcgggccga cccgtcgcgg
     9061 tcgcgggacg agggggggac cggcctgggg ctcgcgatcg tgaagcacct ggtggaggcg
     9121 caggggggac gggtgtgggc cgagagccgg gtcggtgccg gcaccacggt caacgcgtgg
     9181 ctccccgacg tcgtcgacgg tggagccggg gcggcggtgt cgtgatcccg gggatgcggc
     9241 ggacgccacg gcgccggccg gatttacgtg accgtgacag atataccccc ggcgtgtgac
     9301 gcgccggggg cctttttatg cgcgtcggac ggggatcctc cccgatcaca ctcacccaag
     9361 gaaggacagg atgcgactcg ttgctcgtcg gaccatcgcg ctgctcgctg tgttcgccgc
     9421 tatcggcgcg gggcgggcgg ccgcccagca gacccagcct caggtcagcg tcggcggggt
     9481 ggtgctggca cagtatgcgt accaactcaa ggacccggcc gattccaacg cattcgacgt
     9541 cacgcgggcg tacctgaacg tgaccgggaa attctcgggc ggcctgatga cccgggtcac
     9601 caccgacgtc tatcgggccg cggacagctc gaccgtgatc cggctcaagt atgcgtacct
     9661 cgcctggacc cctgagaaga gcccgctgac ctaccgcttc ggactgacgc agacgccgtg
     9721 gatcgactac gaagagaccc tgtacgacta ccggatgcag gggccgaccg tgctcgaccg
     9781 caacggctat atcaccccct cggacttcgg cgccgccgtc gacgggagct ggaatcagga
     9841 caagttcaac ttccaggccg gcgtctataa cggcgaggga tatagcaagg gcagcaccgg
     9901 cgaccagcgc aaggacgtct cggcccgcgc ctcgctgcgc ctgatggaca ccgacgacaa
     9961 cagcagggtc ggcggcctgc gcctgacggc cttcggcgag gcgggcaagc ccaccggcgg
    10021 cgggacccgg aaccgcatca gcgggcaggt ctcgtacaag accaagcagc tcacgctggc
    10081 cggggaatac accgccgcca gcgactcggt cgcggcctcg accagcacca tcaaggggag
    10141 catcctcagc gcgttcggcg tgtacaaggt gccggccagc aaggtggcgc tggtggcgcg
    10201 cctggacgtg gtcgacccga acacgaatgc caccggcgac aagcagaccc ggatcatcgc
    10261 cggggtgtcc taccaggtca cgccgaactg gcgtgcgatg gtggacctcg acaacctgtc
    10321 gtacgagtcg acgccgacgg ccgcgcagga ggccaagcgc tcgaccctgt acttccacac
    10381 ccagctcacc ttctgacctg accacggaac ggagcaccga gacatggctt ccatcgcatc
    10441 cctccgcgcg cggctcggcc tcctgggcct ggccgccgcg gccggcgccg cgccgctcgc
    10501 ggcccagagc ctgaccggcg ccggctcgac cttcggcacc ccgatctaca ccgcctgggc
    10561 ggccgcgctc gaccagaaga ccggcatcca ggtcaactac cagtcgatcg gctcgggcgg
    10621 cggcatccag cagttcaccc agggcacggt cgacttcggc gccaccgacg gcccgatgac
    10681 cgacgcgcag atcgtcgcgg cccaggggaa ggtcctccac atcccgaccg tcctcggcgc
    10741 ggtggtgctc acctggaacc tccccgccct cggcgaccgg aagctcaagc tcgacggccc
    10801 gaccatcgcc gggatctact ctggcgagat cacccgctgg aacgaccggc ggatcgccga
    10861 gctcaacccc gaggtcaagc tcccggcgca ggatatcatc gtggtgcacc ggtcggacgg
    10921 ctcggggacc acgtacatct tcaccgacta cctgagcaag gtgtcgcccg agtggaagca
    10981 gaaggtcggc ttcgccacgt cggtgaactg gccgctgggc ctgggcggca agggcagcga
    11041 aggcgtgacc cagcaggtca agcagaccga aggggccatc ggctacgtgg agctcatcta
    11101 cgccgtgacc aaccacctgc ccttcgcgta cgtcaagaac gccgccggca ccttcgtcga
    11161 cccgtcgctc cagacgacga ccaacgccgc cgccagcgcc cggtggtcca aggacaccga
    11221 cttccgggtg tcgatcacca acgcgccggg caaggaggca tatccgatct cctcgttcac
    11281 ctggctcatc atccgccccg acgtggccga tccggtgaag gcgaaggccc tcaagacctt
    11341 tttcgagtgg atgatcaccc cggaggcgca ggcaatggcg cagaagctcc agtatgcgcc
    11401 gctccccgcg gaagtcgtca agctcatccg cgcccggctc aacaccctca ccagcggtgg
    11461 gaagccgatc aggtgaggta gcgggacagc ggggaagtgg ggcagcgggc cagaaggacc
    11521 gcgggatagc gggacggtcg gccggggtct tcggatcccg gccgactgtc gtcccgatcc
    11581 accgccccat catccgactg acccactgac ccactgtgcc actgccccgc tcccccgttc
    11641 ccccgtgacc catccgttac attcacgtga cggaccgggg ccctttggac ggtaggttcc
    11701 cgctcacatg acacacgccg cctcgacgcc atcaggcctg gaggaacgcc actccggcga
    11761 ccggatcttc cggatcgtcc tgacggcggc cgcggccacg atcccgatcc ttctcgcggc
    11821 actggtgtgg gagctctggg tcggggctct cccggcgatc caccggttcg gcgcctcgtt
    11881 cctggtccgg gacgtctggg atccggtggc gggggatttc ggcgccttcc cgctcatcgt
    11941 cggcacgctg gtctcgtcct tcctggcgct cgtcatcgcc gtcccgctcg ccctgggcgt
    12001 cgcgatcttc ctgacggagt tcgccccccg gtggatccgc cagccgatct cgttcctggt
    12061 cgagctcctg gcggcggtgc cgagcgtggt gtacgggctc tggggcatct tcgtgctggt
    12121 gccgttcctc cggaccacgg tgttcccgtt cctgcgccag gcgctcggct tcctcccgtt
    12181 cttccagggc ccgatctacg gcccgtcgat gctcgccgcg gcggtgatcc tcgcgatcat
    12241 gatcctgccg ttcatcatgt cggtggcgcg cgaggtgctg atggccgtcc ccggcgccca
    12301 gcgggaggcc gcgctggcgc tcggggccac ccgctgggag gcggtggcca cggcggtggt
    12361 cccgtacgcc cgctcgggga tcgtgggtgc tgcgatcctc gggctcggcc gcgccctcgg
    12421 cgagaccatg gccgtcacga tgctgatcgg caacacccac gacatctcgg cctcgctctt
    12481 cgcgccgggc tataccatgg cggccgtgat cgccaacgag ttctccgagg cggcgacgcc
    12541 gctccatttc gcggcgctca cctatgtggc gc
//
LOCUS       DASUAP010000154         6458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_168225, whole genome shotgun sequence.
ACCESSION   DASUAP010000154 DASUAP010000000
VERSION     DASUAP010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168225"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..404)
                     /locus_tag="VFU45_03740"
     CDS             complement(183..404)
                     /locus_tag="VFU45_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358211.1"
                     /translation="MDSAAAFLLPHYRQALAEALASHEQVGCPPCRDVPPELWARTGE
                     AAGWLDPADAIRIRRLAEKVRFFEGRCER"
     gene            complement(477..1892)
                     /locus_tag="VFU45_03745"
     CDS             complement(477..1892)
                     /locus_tag="VFU45_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358212.1"
                     /translation="MPPSASRALSLAVLLAASASAPLAAQLPRFHPPREPAWYQGIES
                     QTLYFVRGTDTTRMTGTAPRIARLTWVPGRHGDALVVTTTALDVHRTVTTDTLRLDAR
                     GNVVAVAADARPARDRYALFLRLPVPPGPLDSGATWGDTLAPGWPAPGVRSLSARITG
                     YHLEGTTDTLGTVTTVVQVDAIARYRQSGWFDSTTGRSFQVDATGPLHETWWLDPKQG
                     ELLARRREFELEGVGLIPGDSGVDSIPVGVSADDAWGRIPAERAQVLARPLPGTDSSW
                     TVTSAPVLLHTVGTLGQAVESGLARNDGSVGTALAETAAARPVQYQALWTSPGAAETT
                     SLVSVRNDTVIATGTTSGRWPAPPMPWAIADEGMEEQLVPILHTLADSQTVKLPTFRP
                     YAGRWDTLAARAVYLTGGARLFLLSRGGPDTRIAILVDSTGGLLTVEKATDPPTRRLP
                     PVGTPRRARLDQLMALLGASD"
     gene            complement(1931..2476)
                     /locus_tag="VFU45_03750"
     CDS             complement(1931..2476)
                     /locus_tag="VFU45_03750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5358213.1"
                     /translation="MPPRTVVTTWLELASPGEFRAPAAIPPDLTLAGPAFQSPELNRF
                     FYTAIGGDWCWTDRLGWSYDDWMEWLSRPGNRTVILSVDGIPAGYAELSQAADTAVEI
                     AYFGLLPQWTGRGLGGAALALVVRHAWEMGAARIVLNTCSLDHPHALENYRARGFRVV
                     RTAESVREVPDTPPGPWPGAR"
     gene            2603..4336
                     /locus_tag="VFU45_03755"
     CDS             2603..4336
                     /locus_tag="VFU45_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU5358214.1"
                     /translation="MVSLHWIDYAVMAVYFAFVLGIGAALRGRMRSSGDFLLSGRSIP
                     AWVAGLAFLSANLGAQEVVGMGASGAKYGIITSHFYWVGAIPAMLFVGLFMMPFYYGS
                     RARSVPEYLRLRFDEKTRAVNALSFAAMTVFSSGISMYAMGKLFHLLLGWDFNVSMLA
                     SAAIVLAYTFLGGLTSAIYNEVLQFFLIVFGFLPLVWIGMRSVGGWAGLTARLAQAAT
                     ARGMAPDVYVRSWAHLGSAAANPIGVEWFGLVMGLGFVLSFGYWCTDFLVVQRAMAAE
                     SMTAARRTPIIAAFPKMLFPFLVILPGMIALALGTGAHGLIPDKLTAAGQPLLDAAGH
                     VVPDFDLATPMLLVKLLPAGLLGLGLTALLASFMSGMAGNVTAFNTVLTYDIYQSYVR
                     PDAPDSHYLHVGRWATVLGVLVSVAAAYVAASFNNIMDVLQLVFAFVNAPLFATFMLG
                     MFWKRATGHGAFAGLVAGILAAAVHHGLTLPVGAEVGVKGGYLGVLAIFPSELAQTFW
                     TAIAAWTTCFVVTIVVSLFTAPRPEAELAGLVYSVTPIPQDAAAPWYRRPAALAAVAL
                     SATVALNLIFW"
     gene            4342..4539
                     /locus_tag="VFU45_03760"
     CDS             4342..4539
                     /locus_tag="VFU45_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358215.1"
                     /translation="MLDLRVPIGGLFALVGAMLLAYGLLHGADPALSPTGIPIVTIWG
                     AVQLVAGLGFLAGARVRPWRR"
     gene            4539..>6458
                     /locus_tag="VFU45_03765"
     CDS             4539..>6458
                     /locus_tag="VFU45_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 35 protein"
                     /protein_id="HEU5358216.1"
                     /translation="MRMLALAAVALSYQLSAISYQPTAIRYQLSAISYSVDTAGSTRH
                     APLATHSLPATRHSPPVRKHRFTIAKGRFLLDGRPFQILSGELHYARIPRAYWAERLA
                     MAKAMGLNAVSVYVFWNFHETRRGHFDWTTDEHDLGAFLDEAKKQGLWVILRPGPYVC
                     AEWDFGGYPSYLARDTSLKVRTRDPRFLAVEKRYIRALAKVITPRLVTHGGPVVLVQV
                     ENEYGSFGSDHRYLQLTRQMFQDAGFNVPLFTADGDGLFDKGAIPGVLPGANGETNYD
                     SLVARVDRFHRGKGPYFVPEFYPGWLDHWGEKFVRVATRDFIGSYDTLISRGASVNLY
                     MFHGGTNFGFTSGANSSADAPIQPSLTSYDYDAPLDEAGHPTPKYYALRALLAKRSKR
                     PLPPVPTPAPVISIPDIPLSAAGGLYDLVDTLHDVHARRPLTFEELGQRAGYVLYRHQ
                     VDSAAYGMLEVPGLRDFGLVFVNGRRVAMLDRRTGTFSTVVDVPARGELEILVESEGR
                     INYGPEVQQNRKGIISPVRLAGTELKGWRMARLPFDSLPAPIAKPAPEVESVPTLYRG
                     SFTLDSLGDSFLDLRGWGKGIVFVNGHNLGRYWDVIGPQRTLYLPGPWLRKGQNDIAV
                     FEQLNDSVPGHVAGLT"
BASE COUNT          918 a         2134 c         2353 g         1053 t
ORIGIN      
        1 gaaaagggaa gagggcaagc cgatccttcg ggccttcggc cctcaggatg acgttgcacc
       61 ggacgtccgc tctccgttcc cctttgctcg ttcgccgttc gccgttcacc gttgaccgtt
      121 gaccgtttac cgttcaccgc tctctcttgc tcgttccccg ttgaccgttg accgttcacc
      181 gttcaccgct cacatctccc ctcgaagaac cgcaccttct ccgccagacg ccggatccgg
      241 atggcgtcgg ccgggtcgag ccagccggca gcttcgccgg tccgggccca gagttccggc
      301 ggcacgtcgc ggcagggcgg gcatccgacc tgctcgtggg atgccagcgc ctcagcaagc
      361 gcctggcggt agtgggggag caggaatgca gctgccgagt ccatcgccca agatgaggcc
      421 ggaccggtcg agggcgataa ccggaagttt gcaggatggt gcggatctcc gccggttcag
      481 tccgatgcgc cgaggagtgc catcagctgg tcgaggcggg cgcggcgggg tgtgcccacg
      541 ggtggcaggc ggcgggtcgg ggggtcggtg gccttctcga cggtgaggag accgccggtc
      601 gaatcgacca ggatcgcgat ccgcgtgtcg gggccgccgc gcgagagcag gaagagcctg
      661 gcgccgccgg tcaggtacac ggcgcgtgcg gcgagcgtgt cccagcgccc ggcgtagggc
      721 cggaaggtcg ggagcttcac ggtctgggag tcggccaggg tgtggaggat gggcacgagc
      781 tgctcttcca ttccctcatc ggcgatggcc cacggcatcg gcggggcggg ccagcgtccc
      841 gatgtcgtgc cggtcgcgat gaccgtgtca ttccggactg acaccagaga ggtcgtttcc
      901 gccgcgcccg gtgaggtcca gagcgcctga tactgtaccg ggcgggccgc ggcggtctcg
      961 gcgagcgccg tgccgaccga tccgtcgttc cgcgcaaggc ccgattcgac cgcctggccc
     1021 agcgtgccga cggtgtgcag gaggaccggt gcgctcgtga cagtccacga actgtcggtg
     1081 cccgggaggg gtcgggcgag cacctgcgcg cgctcggcgg ggatgcgccc ccaggcgtcg
     1141 tcggcgctga cgcccacggg gatcgagtcc acgccggaat cgcccgggat caggcccacc
     1201 ccttcgagct cgaactcccg gcggcgggcg aggagttcgc cctgcttcgg gtcgagccac
     1261 caggtctcgt ggagcggtcc tgtcgcgtcg acctggaagg agcgtccggt ggtggagtcg
     1321 aaccatcccg actgccggta gcgagcgatg gcgtccacct gcaccaccgt ggtgacagtg
     1381 ccgagcgtgt cggtggtgcc ttcgaggtga tagccggtga tgcgggcgga gagggagcgg
     1441 acgccgggag cgggccatcc gggggcgagc gtgtcgcccc aggtggcgcc ggaatcgagc
     1501 gggccgggcg gcaccgggag ccgcaggaag agggcgtacc gatcgcgggc gggtcgagcg
     1561 tcagcggcga cggccaccac gttgccccgc gcgtcgaggc ggagcgtgtc ggtggtgacg
     1621 gtgcggtgga cgtcgagcgc ggtggtcgtg accacgagcg cgtcgccgtg gcgcccgggg
     1681 acccaggtca gccgggcgat gcggggcgcc gtgccggtca tccgggtggt gtcagtgccg
     1741 cggacgaagt agagggtctg ggactcgatc ccctggtacc aggccggttc cctgggtgga
     1801 tggaaccggg ggagctgggc ggcgagcggt gcactggcac tggcggcaag aaggacggcg
     1861 agcgacagcg cgcgggacgc ggatggcggc atggagcgga agttagcggg aagagcggct
     1921 cggttgccgc tcagcgggcg ccgggccacg gaccgggggg cgtatcgggg acctcccgca
     1981 ccgattcggc ggtgcggacc acccggaatc cgcgggcgcg gtagttctcg agcgcgtgcg
     2041 ggtggtcgag cgagcaggtg ttgaggacga tccgcgcggc acccatttcc caggcgtggc
     2101 ggacgacgag ggcgagggcg gcgccgccga gtccacggcc ggtccactgc ggcaggagcc
     2161 cgaagtaggc gatctcgacc gccgtatcgg cggcctgcga aagttcggcg taccccgcag
     2221 ggatcccgtc caccgacagg atcacggtcc ggttgcccgg gcgggagagc cattccatcc
     2281 agtcgtcgta gctccagcca agccggtcgg tccagcacca gtcgccgccg atggcggtgt
     2341 agaagaagcg gttgagttcg ggggactgga acgccgggcc ggcgagcgtc aggtcggggg
     2401 ggatcgcagc gggggcgcgg aattcgccgg gggaggcgag ttcgagccag gtggtcacga
     2461 ccgtgcgcgg cggcatcggc acccgggaaa ggggaggccg cctggccggg cgagcggctc
     2521 gcgcggcggg gcggcagcgt acatactaca gcacgcccgc agcgcacgcg agcccacctc
     2581 ccacctccag acccggcagc gcatggtctc cctccattgg atcgactacg ccgtgatggc
     2641 ggtgtacttc gccttcgtgc tcggcatcgg cgcggcattg cgcgggcgga tgcggagcag
     2701 cggggacttc ctgctctcgg ggcgctccat ccccgcatgg gtggcgggac ttgcctttct
     2761 ctccgccaac ctgggggcgc aggaagtggt ggggatgggc gcgtcggggg ccaagtacgg
     2821 gatcatcacc agccatttct actgggtcgg tgccatcccg gcgatgcttt tcgtcgggct
     2881 cttcatgatg ccgttctatt atggatcgcg cgcgcggtcg gtgccggaat acctgcggct
     2941 ccggttcgac gagaagaccc gcgcggtcaa cgcgctctcg ttcgcggcga tgaccgtgtt
     3001 ctcctccggc atctcgatgt acgcgatggg caagcttttc cacctcctcc tcggctggga
     3061 cttcaacgtc agcatgctcg cctcggcggc gatcgtgctc gcctacacct tcctgggcgg
     3121 gctcaccagc gccatctaca acgaggtgct gcagttcttc ctgatcgtct tcggattcct
     3181 gccgctggtg tggatcggga tgcggagcgt gggggggtgg gcggggctca ccgcgcggct
     3241 ggcgcaggcg gccacggcgc gggggatggc gccggacgtg tacgtgcgga gctgggcgca
     3301 cctcgggagc gccgccgcca atcccatcgg ggtcgagtgg ttcgggctgg tgatgggact
     3361 cggcttcgtg ctgtcgttcg gctactggtg caccgacttc ctggtggtcc agcgggcgat
     3421 ggcggccgag tcgatgaccg ccgcgcgccg gacgcccatc atcgccgcgt tccccaagat
     3481 gctcttcccg ttcctggtca tcctgcccgg gatgatcgcc ctggcgctcg gcaccggcgc
     3541 gcacgggctc attcccgaca agctcacggc ggccggccag ccgctcctcg acgccgccgg
     3601 ccacgtggtg cccgacttcg acctcgcgac cccgatgctc ctggtcaagc tcctcccggc
     3661 ggggctcctg gggctcgggc tcaccgctct ccttgcctcg ttcatgagcg ggatggccgg
     3721 gaacgtgacc gcgttcaaca ccgtgctcac gtacgacatc taccagagct acgtgcgacc
     3781 ggacgctccc gactcgcatt acctccacgt ggggcgctgg gccacggtgc tcggcgtgct
     3841 ggtatcggtg gcggccgcgt acgtggcggc atcgttcaac aacatcatgg acgtgctgca
     3901 gctggtgttc gcgttcgtga acgcgccgct ctttgccacg ttcatgctgg ggatgttctg
     3961 gaaacgcgcc accggacacg gcgccttcgc gggtctggtg gcggggatac tcgcggcggc
     4021 ggtgcaccac gggttgacgc tcccggtcgg tgcggaggtc ggcgtcaagg gaggctacct
     4081 cggcgtcttg gccatcttcc cgagcgagct ggcccagacc ttctggaccg cgatcgcggc
     4141 gtggaccacc tgctttgtgg tgaccatcgt ggtgagcctc ttcaccgcac cgcggccgga
     4201 ggccgaactg gccgggctcg tctactcggt gacgccgatc ccgcaggacg ccgcggcgcc
     4261 ctggtaccgg cgccccgcgg cgctcgccgc ggtggcactc agcgccacgg tggcgctcaa
     4321 tctcatcttc tggtgacggc gatgctcgac ctgcgtgtcc ccatcggcgg gctctttgcc
     4381 ctggtcggcg cgatgctgct ggcatacggc ctcctgcacg gtgccgatcc ggcgctcagc
     4441 cccaccggga tcccgatcgt gacgatctgg ggcgcggtac agctcgtggc gggactcgga
     4501 ttcctggcag gggcgcgggt ccggccgtgg aggcggtgat gaggatgctg gcattggcgg
     4561 cggtggcgct gagctatcag ctatcagcta tcagctatca gccgaccgct atccgctatc
     4621 agctatcagc tatcagctac tccgtggata cggcgggctc cacccgccac gcgccactcg
     4681 ccacccactc gctacccgcc acccgccact cgccaccggt ccggaagcac cggttcacga
     4741 tcgcgaaggg caggttcctc ctcgacggcc ggccgttcca gatcctctcc ggcgagctcc
     4801 actacgcgcg gatcccgcgg gcgtactggg cggaacggct ggcgatggcc aaggcgatgg
     4861 ggctcaacgc cgtgtcggtg tacgtcttct ggaactttca cgagacccga cggggccatt
     4921 tcgactggac caccgacgag cacgatctcg gcgcttttct tgacgaggcg aagaagcagg
     4981 ggctctgggt catcctgcgg cccgggccgt atgtgtgcgc ggaatgggac ttcggcgggt
     5041 atccgtcgta tttggccagg gacacgtcgc tcaaggtgcg cacgcgcgat ccgcgtttcc
     5101 tcgccgtgga gaagcgctac atccgggcgc tggcgaaggt gatcacgcca aggctcgtga
     5161 cccacggcgg gccggtggtg ctggtgcagg tggagaacga gtacggctcc ttcgggagcg
     5221 accatagata cctgcagctc accaggcaga tgttccagga cgcggggttc aatgtccccc
     5281 tcttcaccgc ggacggggac gggctgttcg acaaaggagc catccccggc gtccttcccg
     5341 gcgccaacgg ggagaccaac tacgactccc tcgtggcgcg ggtcgaccgg ttccaccggg
     5401 ggaaggggcc gtacttcgtg ccggagttct atcccgggtg gctcgaccac tggggggaga
     5461 agttcgtccg cgtcgcgacc agggatttca tcgggtcgta cgacacgctc atttcccggg
     5521 gggccagcgt caacctctac atgttccacg gcggcaccaa cttcgggttc acctccggcg
     5581 ccaattccag cgccgatgcg ccgatccagc cctccctcac gagctacgac tacgacgcgc
     5641 cgctcgacga ggcgggccat ccgacgccca agtactacgc cctgcgggcg ctcctggcca
     5701 agcggtcgaa gcgaccgctt ccgccggtgc cgaccccggc gccggtgatc tcgatcccgg
     5761 atatcccgct ctccgcagcg ggcggactct acgacctggt cgacaccctt cacgacgtcc
     5821 acgcacggcg gccgctcacc ttcgaggagc tgggccagcg ggccggctac gtcctgtacc
     5881 ggcaccaggt cgactccgcg gcctatggga tgctcgaggt gccggggctg cgggacttcg
     5941 ggctcgtctt cgtcaacggg cggcgggtgg ccatgctcga ccggcggacc ggcaccttct
     6001 cgaccgtggt ggacgtgccg gccagggggg aactcgagat cctggtggag agcgaagggc
     6061 ggatcaatta cggaccggag gtgcagcaga accggaaggg tatcatctcg ccggtgcggc
     6121 tggcgggcac ggagttgaag ggctggcgga tggcgcggct gccgttcgac agcctccccg
     6181 cgccgatcgc caagccggca ccggaagtgg agagcgtccc cacgctctac cgggggagct
     6241 ttaccctcga ctcgctcggc gacagcttcc tcgacctcag ggggtggggg aaggggatcg
     6301 tcttcgtcaa cgggcacaac ctcggccgct actgggacgt gatcggtccg cagcggacgc
     6361 tctacctgcc ggggccatgg ctccgcaagg ggcagaacga catcgccgtg ttcgagcagc
     6421 tcaacgactc ggtgccggga cacgtggcgg ggctcacg
//
LOCUS       DASUAP010000155         4960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1121724, whole genome shotgun sequence.
ACCESSION   DASUAP010000155 DASUAP010000000
VERSION     DASUAP010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121724"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFU45_03770"
     CDS             <1..570
                     /locus_tag="VFU45_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU5358217.1"
                     /translation="VDVRVLAATNKRLEEEIAAGRFREDLFYRLNVVPIHVPPLRERR
                     EDIPVLVRHFAGTLARESGLPARKFEEGALRALERRAWPGNVRELRNAVERLLILADG
                     KAVTGADVERVLGGGEAGAPEADTDEDAAGDGGDGSFEQFKVEAEKAFLAQKLREHEW
                     NVSETARALRMPRSNLYKKIERYGLSRDG"
     gene            570..1022
                     /locus_tag="VFU45_03775"
     CDS             570..1022
                     /locus_tag="VFU45_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358218.1"
                     /translation="MADQPRDWDKEMAAIDREIARLPEPKPGQPPAARAGGVPAGPPS
                     PAAPHPTGARAFLGAWIRVLLGAAVAVAVWSWPYGHPCGWSLYGYLGAVLVVIVAGLW
                     NILATWKRRMGAAHAVAIVVFLTGLFLAAQAILPRVGYARQVLTWMCP"
     gene            1019..1534
                     /locus_tag="VFU45_03780"
     CDS             1019..1534
                     /locus_tag="VFU45_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5358219.1"
                     /translation="MSAADAFVVPIRAVLLRDLAAFRRELEAYPDEASIWQLPAGVPN
                     SAGTLALHVAGNLRHFVGAILGGSGYVRDRAAEFSERGVPRAELLRRLVAAEDAVRRG
                     LDGRALPEQYPGAAGGGGMTVRTDEWLAHLAVHLTYHLGQVDYHRRTVTGRSTGVGAI
                     ATAELPSARTP"
     gene            1584..3074
                     /locus_tag="VFU45_03785"
     CDS             1584..3074
                     /locus_tag="VFU45_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5358220.1"
                     /translation="MPTFKNFIGGRWVVPSTARYFEDRNPADVRDLIGKFPDSGARDV
                     AEAVKSARRGFELWKRTPAPARGDVLRRLGDLLVAHKEELADGMTREMGKVLAETRGD
                     VQEGIDTAYYAASEGRRLFGHTVPSELRDKWAMSYRRPIGVCGLVTPFNFPMAIPTWK
                     MFPALLCGNAVIIKPSEDVPHTVHRLVELVLEAGLPPEVIQLVHGRGAVAGKALVEHP
                     EVPVISFTGSTQTGSFIGETCGRMHKRLSLEMGGKNAMIVMDDADLDLALDGVLWGAF
                     GTTGQRCTATSRLIVHRKVHERFVARVADAAAKLRLGDGRKAGTDVGPLIHEAAREKV
                     ERYVEIGRAEGLELVTGGKRPAGAGTAGGWFYRPTIFTGATSKSRLAQEEIFGPVLTV
                     LPVGSLDEAIRVNNSVKYGLSSSLYTRNVNAAFRALQDLDNGITYINAPTIGAEAHLP
                     FGGVKQTGNGHREGGWEVYEFYSETKVGYVDYSGTLQRAQIDNYDG"
     gene            3357..4220
                     /locus_tag="VFU45_03790"
     CDS             3357..4220
                     /locus_tag="VFU45_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5358221.1"
                     /translation="MQVGTGKRGPSFADEGSLDQYLREISKYPLISQDEEVDLAQRIR
                     KGEAEALDKLVRSNLRFVVSVSKKYQNQGVSLSDLINEGNLGLIRAAHKFDETKGIKF
                     ISYAVWWIRQAILQALAEQSRIVRVPLNRAGTLHRIGKRSSALLQELGREPTPSEIAE
                     GMDISMEEVQKTLSISQNHLSLDAPMTPGEDNRLLDYLPDTQNPGPDAETFEHALTES
                     IEEVLGTLKEREAKILRLYFGLDGPEPMTLEEIGSLLGITRERVRQIKEKALTRLRHV
                     SRAKALESFLA"
     gene            4310..4813
                     /locus_tag="VFU45_03795"
     CDS             4310..4813
                     /locus_tag="VFU45_03795"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEU5358222.1"
                     /translation="MASNPSFDLSTGADLQEVDNAVNQVLKEIAQRYDFKGTHCTVEF
                     DRSKAEIRLAADDAFRMESLLDVLRTKLTKRGVPLKNLEVGEDIPGAGESVRRVATLK
                     QAIDKDNAKAIVKAIKDQGFKKVQASIQGDEVRVTSPSRDDLQAVIAFLRTQDFGIEL
                     KFGNFRS"
BASE COUNT          780 a         1685 c         1796 g          699 t
ORIGIN      
        1 gtggacgtcc gcgtgctggc ggccaccaac aagcggctcg aagaggagat cgcggccggc
       61 cggttccgcg aggacctgtt ctatcggctc aacgtcgtgc cgatccacgt cccgcccctg
      121 cgggagcggc gggaggacat cccggtcctg gtgcgtcact tcgccgggac gctcgcccgg
      181 gagagcggcc tcccggcgcg caagttcgag gaaggggcgc tccgcgcgct cgaacggcgg
      241 gcatggcccg gcaacgtgcg cgagctccgc aacgcggtcg aacggctgct gatcctggcg
      301 gacggcaagg cggtgaccgg cgccgacgtc gagcgggtgc tgggcggggg ggaggcggga
      361 gcgccggagg cggataccga cgaggacgcc gccggcgacg gcggggatgg cagcttcgag
      421 cagttcaagg tggaggcgga gaaggcgttc ctggcgcaga agctccggga gcacgagtgg
      481 aacgtctccg agacggcgcg ggcgctccgg atgccgcgct ccaacctgta caagaagatc
      541 gaacgctacg gcctcagccg ggacggatga tggcggacca gccacgcgat tgggacaagg
      601 aaatggcggc gatcgaccgt gagatcgcca ggcttcccga gccgaagccg gggcaacccc
      661 ctgcggcccg tgcgggcggc gtgccggcgg ggccgccctc gccggccgcg ccacacccga
      721 ccggcgcgcg cgcgttcctc ggcgcgtgga tccgggtgct cctgggtgcc gcggtggcgg
      781 tggccgtctg gtcgtggccg tacggccatc cctgcggctg gtcgctctac gggtatctcg
      841 gcgcagtgct cgtggtcatc gtcgcgggcc tctggaacat cctggccacc tggaagcggc
      901 ggatgggcgc cgcgcacgcg gtggcgatcg tcgtgttcct caccgggctc ttcctggcgg
      961 cacaggcgat cctgccccgc gtcggctacg ccaggcaggt gctgacctgg atgtgtccgt
     1021 gagcgccgcc gatgcgttcg tggtcccgat ccgggccgtg ctgctgcgcg acctggccgc
     1081 gttccggcgg gagctcgagg cgtacccgga tgaggcctcg atctggcagc tgcccgccgg
     1141 cgtccccaac tccgcgggga cgctggcgct ccacgtggcg gggaacctgc ggcacttcgt
     1201 gggcgccatc ctgggcggga gcggttacgt gcgcgaccgg gccgcggagt tctccgagcg
     1261 cggtgtgccg cgcgccgagc tgctccgtcg cctggtggcg gccgaggacg cggtgcggcg
     1321 aggactggac ggccgcgcac tgccggagca gtatccgggc gcggcagggg gcggggggat
     1381 gaccgtccgc accgacgaat ggctggccca cctggccgtc catctgacgt atcacctggg
     1441 ccaggtggat tatcatcgcc ggaccgtcac cggccgttcc accggcgtcg gcgccatcgc
     1501 caccgccgag cttccgtccg cccgcacacc ctgagccggc catcggctcc cgtcgccacc
     1561 ctcagaccac gaatcgtccc tccatgccca cgttcaagaa cttcatcggc ggccgttggg
     1621 tcgtcccgtc gacggccagg tacttcgagg accggaatcc ggccgacgtc cgcgacctga
     1681 tcgggaagtt ccccgactcc ggcgcgcggg acgtcgccga ggcggtcaag agcgccaggc
     1741 ggggcttcga gctctggaag cggacacccg ccccggcccg gggcgacgtg ctgcggcgtc
     1801 tcggcgacct cctggtggcc cacaaggagg agctggccga cgggatgacg cgcgagatgg
     1861 ggaaggtgct ggcggagaca cggggggacg tgcaggaggg gatcgacacg gcgtactacg
     1921 cggcgagcga ggggcggcgg ctgttcgggc acacggtgcc gtcggagctc cgggacaagt
     1981 gggccatgag ctaccgccgg ccgatcggcg tctgcggact ggtgacgccc ttcaacttcc
     2041 cgatggcgat cccgacctgg aagatgttcc ccgcgctcct ctgcgggaac gcggtcatca
     2101 tcaagccgag cgaggacgtg ccccacacgg tgcaccggct ggtggagctg gtgctggagg
     2161 cggggctccc gccggaggtg atccagctgg tgcacggccg gggcgcggtg gcggggaagg
     2221 cgctggttga gcacccggag gtgccggtca tctcgttcac cggctccacg cagaccgggt
     2281 cgttcatcgg tgagacctgc ggccggatgc acaagcgcct ctcgctcgag atggggggca
     2341 agaacgcgat gatcgtgatg gacgacgccg acctcgatct cgcgctcgac ggggtgctgt
     2401 ggggggcgtt cgggaccacc ggccagcggt gcaccgccac cagccggctc atcgtgcatc
     2461 gcaaggtcca cgaacggttc gtggcccgcg tggccgacgc cgcggcgaag ttgcgcctgg
     2521 gcgacggccg gaaggccggc accgacgtcg gcccgctgat ccacgaggcg gcgcgggaga
     2581 aggtcgagcg ctatgtggag atcggccggg ccgaggggct cgagctcgtc accggcggga
     2641 agcgaccggc gggggcgggg acggccgggg ggtggttcta ccggccgacg atcttcaccg
     2701 gtgccacgtc gaagagccgg ctggcccagg aggagatctt cggtccggtg ctcacggtgc
     2761 tccccgtggg atcgctcgac gaggccatcc gggtcaacaa ctcggtcaag tacggtctct
     2821 cgagctccct ctacacgcgg aacgtgaacg cggcgttccg cgcgctgcag gacctggaca
     2881 acgggatcac ctacatcaac gccccgacga tcggtgccga ggcgcacctg cccttcggcg
     2941 gcgtcaagca gaccggcaac ggacaccggg agggcgggtg ggaggtctac gagttctact
     3001 cggagaccaa ggtgggatac gtcgactact caggcaccct gcagcgggcg cagatcgaca
     3061 attacgatgg gtgaacaggc acggtgaatg ggcgtgacgt ggctcacatc gccccggtac
     3121 cgttcaccgt tgaccgttca ccgcgctccg gcccgttgcg cgctcctgtc gccccggcta
     3181 aggttccgga agctggacgc ggcgcccgca cacgcgggcg cgggcgggtg ggatcttccc
     3241 ggtggataac gcgccaagac tgctcgaccc ccgcgctgct gccgagcgcc gccgctggcg
     3301 gcgccggggc cgctgaaccg tcccgttccg tcgagatctc ctgaccgatg cgcccgatgc
     3361 aagtcggaac tggcaagcgg ggcccgtcct tcgcggatga gggctcgctc gaccagtatc
     3421 tgcgcgagat cagcaagtat ccgctgatct cccaggacga ggaggtcgac ctggcccagc
     3481 gcatccgcaa gggcgaggcc gaggcgctcg acaagctggt ccgcagcaac ctccgtttcg
     3541 tggtgtcggt ctccaagaaa taccagaacc agggcgtctc gctctccgac ctgatcaacg
     3601 agggcaacct cgggctcatc cgcgccgccc acaagttcga cgagacgaag ggcatcaagt
     3661 tcatctccta cgccgtctgg tggatccggc aggccatcct gcaggcgctg gcggagcaga
     3721 gccggatcgt gcgggtgccg ctcaatcgcg ccgggaccct gcaccgcatc ggcaagcgct
     3781 cgagcgcgct gctgcaggag ctggggcggg agccgacgcc gtcggagatc gccgagggga
     3841 tggacatctc gatggaggag gtgcagaaga cgctctccat ctcccagaat cacctgtcgc
     3901 tcgatgcgcc gatgaccccg ggcgaggaca accgcctcct cgactacctg cccgacaccc
     3961 agaaccccgg tcccgacgcc gagaccttcg agcacgcgct caccgagtcg atcgaggagg
     4021 tgctcggcac cctcaaggag cgcgaggcga agatcctccg tctctacttc ggcctcgacg
     4081 gcccggagcc gatgaccctc gaggagatcg gcagcctcct cggcatcacc cgggagcggg
     4141 tccgccagat caaggaaaag gccctcaccc ggctccgcca cgtgagccgg gccaaggccc
     4201 tcgagagctt cctggcgtag gcggcgaccg cggggaacga ccaacgcgcg tctcccatcg
     4261 cgctccgtcc ccgtttactg ttgaccgttg accgttcacc gggacgcgca tggcctcaaa
     4321 cccctcattc gacctatcga ccggcgccga cctccaggag gtcgacaatg cggtcaacca
     4381 ggtgctgaag gagatcgccc agcgctacga cttcaagggc acccactgca ccgtggagtt
     4441 cgatcggtcc aaggccgaga tccgcctggc cgccgacgac gccttccgga tggaatccct
     4501 cctcgacgtc ctgcgcacca agctcacgaa gcggggcgtg ccgctgaaga acctcgaggt
     4561 cggggaggac atccccggcg ccggggagag cgtgcgccgg gtggcgacgc tcaagcaggc
     4621 catcgacaag gacaacgcca aggccatcgt caaggccatc aaggaccagg ggttcaagaa
     4681 ggtgcaggca tcgatccagg gcgatgaagt ccgggtgacc agcccgtccc gtgatgacct
     4741 ccaggccgtc atcgcgttcc tgcgcacgca ggacttcggg atcgagctca agttcgggaa
     4801 cttccgcagc tgacgcccgc gtggagggcc ggcatcctgg cggccgctct cgcgtcggcg
     4861 tgcgcatcgc cggccaaccc accgatcctg gcacccggtg gtggcgccgg cgagccgctg
     4921 atgtcggtgg ggctcctgga ggaggcgccc cgggtgcagg
//
LOCUS       DASUAP010000156        16961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_712932, whole genome shotgun sequence.
ACCESSION   DASUAP010000156 DASUAP010000000
VERSION     DASUAP010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 16961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16961
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712932"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1923
                     /gene="pilB"
                     /locus_tag="VFU45_03800"
     CDS             208..1923
                     /gene="pilB"
                     /locus_tag="VFU45_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEU5358223.1"
                     /translation="MATAAAGNERLGDILVREGLINRDQLSQALLEQKSSGHRLGYVL
                     VKLGLVQELEVTKVLAKQYRMPAVDLSRFEVDPKIIKLVPADLALKKSVLPLKREGRT
                     LTVAMADPTDSGLIEDLKFITRYDLFPVIAGEYTLRSLIEKHYNSGDQQLADLLKEME
                     GEGETDDVEVVKEEEDEQASQAQIDDAPVVKLINTILTEAVKRGASDIHIEPFEHEIR
                     VRFRIDGALMEIMKPPIKMKAALTSRVKILSQLNIAERRVPQDGRLRLKMGARVIDFR
                     VSTVPVLFGEKIVLRILDKGNLTLDLSKFGFEPKAEQDLMRNIMNPYGMVLVTGPTGS
                     GKTTTLYSALSQVNKVDVNIMTAEDPVEYNLMGINQVLVRNEVGLTFAAALKAFLRQD
                     PNIIMIGEIRDLETGGIAIKAALTGHLVLSTLHTNDAPSTITRMIDMGIEAFNVASAV
                     NLVVAQRLVRRICKNCKAPAKYKPEEIATLIPNPEDAAGIEFMKGTGCDTCNGSGYKG
                     RAGLYEVMALSPELRRLILRGASVVELRDQGVAEGMLTLRMDGIVKVKKGVTTLEEVI
                     KETAG"
     gene            1920..3044
                     /locus_tag="VFU45_03805"
     CDS             1920..3044
                     /locus_tag="VFU45_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEU5358224.1"
                     /translation="MTVNLRSLLEEMIEREASDLHITAGERPKLRVDGDIVNSSCEEV
                     LTPKDTLSLAYSVLTENQKKRFENDNELDFSFGIQNLARFRGNVFKQRGCVAVVVRMI
                     PFSVQTFQELGLSPIIAKLAERPRGLVLVTGPTGSGKSTTLAAMIDKINKERKGHIIT
                     VEDPIEFIHRHQQCIINQREVGTDTKSFSSALKYALREDPDVILIGEMRDLDTIGAAL
                     TIAETGHLAFATLHTNSAAEAINRIIDVFPSNQQSQVRAQLAFVLEGIITQTLLQKSK
                     GKGRVMAAEIMVATPAIRALIRDDKIHQIYSAMQSGKKHGMQTMNDALYQHYTNRDIT
                     LEEALRVTHDPVEFQRMVGITPAEEDFGTADKPQRPALKR"
     gene            3063..4268
                     /locus_tag="VFU45_03810"
     CDS             3063..4268
                     /locus_tag="VFU45_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5358225.1"
                     /translation="MPIFEYSARNPQNNQILKGTIDAPNKDEAAKQLRQKKLLLVNLR
                     EQPKQLKLNFGGGGIPTRDVVIFARQFATMINAGLPLVQSLTVLSEQTENKALGQVAR
                     EVVYDVESGNTLADAMAKHPKAFSELFVNMVAAGEAGGILDTILGRLAGFLEKNDALV
                     RKVKGAMVYPVVIMSVAAIAICVLLIFVIPTFQTMFASVNMELPLPTRIVIGASHILL
                     GFWWAIILAVGGTVFGIKKYYTTADGKKRIDTIMLQLPILGDLIRKSAVSRFTRTLGT
                     LLSSGVSILDGLEITAKTAGNRVIHDAVMASRQSIAGGETISAPLAKSKVFPPMVVSM
                     IAVGEQTGGLDEMLSKIADFYDEEVDVAVGALLSLMEPVMIVILGVVVGGMVVAMYLP
                     IFGMINTVQ"
     gene            4376..6184
                     /locus_tag="VFU45_03815"
     CDS             4376..6184
                     /locus_tag="VFU45_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5358226.1"
                     /translation="MIQLRRTLVPAAAGLRAFWPTAMLVVATATVALMALLPVASLSG
                     ATSVFLPRLSLPAAPAIDLGIRWSSVAQGPSDLHALGITALFRLLLGVAAALAGVALL
                     AISAVAAARAQQRQGEVSIHRSVGASRRHLVLSHGAEGVVVSLLGLGVGAVLGRTIAV
                     CAARYWPGHLAPTGPLPGISAAAALAGAMVLGALFPLVYARRRVPRTAVGGEPLGLAI
                     PTFQLGLSLTVLVAAVLLWRGAGGSARRGPSDTDLTVIHLAALTGSAAAARSAQYAGV
                     LAHVRSAAGVRIAALTSPGTVSGAGESDIAVVHCGDGCSLGGLPLPYQTARAMHYAVS
                     PDSFNAMGLPIIAGRGIADTDTWRAPRVAVVNQAFAIAHFERGDPLGHELRLGAGPWS
                     RFVVVGVVADQPVEGVGGGAAPANAIYVSVLQQPPPNVDLLVRGGVPAAVAGAARAAL
                     GPAAGPLRTTSNSEIRATQTAPVRWFAALFGLVGCALLIVATAGTATVVHQWVASLAV
                     ELGVRRAAGASRQQVVAFVLIRLIAMAGVGAMIGVWLGTGVWGTLHQAVPGVPAWDAR
                     TVLEAAGVLVGAAMAGGLAPAVRAALASPAGLMDSA"
     gene            complement(6205..7230)
                     /locus_tag="VFU45_03820"
     CDS             complement(6205..7230)
                     /locus_tag="VFU45_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5358227.1"
                     /translation="MTDHAPLPTRPLGTTGMEITRVGLGAWAIGGGDWQFGWGHQDDA
                     ESVAAIRHAVARGINWIDTAAVYGLGHSEDVVARALRGLPASERPYVFTKCGMVWDEA
                     DRSKPPARVASPASIRKELEGSLRRLGVERIDLYQLHWPPEDGTPLEEYWGAMLELKR
                     EGKIRAAGLSNHKADQLAAAEKLGHVDSLQPPFSAVRRDVAERELPWCADHRTGVIVY
                     SPMLSGLLSGSFTRERAAALPKNDWRSRAVNFQGEALERNLALVETLRGVAGRRGVGV
                     GAVAIAWTLAWRGVTGAIVGARSPAQVDGWIAAASLELTADDLTEIGSAIEKTGAGSG
                     PIGPPVR"
     gene            complement(7227..10043)
                     /locus_tag="VFU45_03825"
     CDS             complement(7227..10043)
                     /locus_tag="VFU45_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358228.1"
                     /translation="MIALIPSLFALTTALGAGGPVPPRSAVEAAFGPRGLTEIRDRRS
                     GAAWRLRTDGFRLVIDGKAYDSADLPAPARSAAGGRTTYRWKAGAYVIEVVYETRPDW
                     AFVSKQLTVTGSGAWRVDSVTPLRLGFEVAPADVHTIPATNGELDTGAWGAAIRGDRG
                     PGLLALVQDPFLSIGRAGGLVTVSYAPAMPWNAADGAFPADRALLVPYQATGRRLPAA
                     MAPEWAWHPAVTADGLDEAEVLAFTDAVRAFLIAPPAHPTDVFVGWTANDYQIDVGTA
                     AGQAEYRRVLDRAAELGARDVLYAPANSTLSTRDESVDDWHWEYVLWFGLGPRIRRGE
                     WRPDRDTLPASIKGMIDYARSRGLKLLAYVYPVMPFAADSSWIVSPPDAPDRHYANLA
                     SRALQDWLITQLTAFYRRNGIGGYSFDHTFLDLPGAGTYAQWWGWRRVMEELKRRNPG
                     IVIDGRQAYQLYGPWSWLAGTYPHPTGTDEQPESFVPFPDLHIDRVSADRERYTAYRY
                     RNYEFAPSELVPGFMTHQTPRADATGRMPERRTARGGELTSFRTRDWDYLGWRYSVLS
                     SIAVAGWNNVINDIPARDLAEDTLFRAEDGAWLRGWLTWADAHRELLRRTRTILGPPG
                     FGKVDGTSAVDGDSGYIFLFNPNAIPERASLPLGGAIGLPARGRWIVTEREPLSGRRL
                     ASPDRGIWSAQDTMTVTVPPVTALVLQVAPAPARIDAPILFGVPGSARLAGDTLALDG
                     MRGDAGTDADATILLPAGARVTTVRVDGAVAADARIAGGMVTVPVRFAGAAFGADARV
                     GAYDPTFTGGPWQATFAVPARVFDQLARRARQWPVPWTAEDHATPWLVPERLLLYAQF
                     AEPDDRWELRMWIDGRPVEVKKAYSSVRPVPHDFVGFYADVSLLTADRPHHIELEVPQ
                     MRAGQFQGLFFENVEPEYTTAAPR"
     gene            10190..13423
                     /locus_tag="VFU45_03830"
     CDS             10190..13423
                     /locus_tag="VFU45_03830"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HEU5358229.1"
                     /translation="MLLLLFALAASVASAQSRLVTRWAAAVDTLHPLPEYPRPEMRRS
                     SWLDLNGPWDYAIQDGHAARPEHWDGRIVVPFAVESQLSGVRRPVLPSQALWYRRRVE
                     VRPAAGTRWLLHFGAVDWSTTVWVNGKVVGRHVGGYDPFTFDVTAALAPSGSQEIVVQ
                     VTDPTDSGGQPRGKQVLKPGSIWYTAVTGIWQTVWLEPVPVAHIERLGIVPDLADGRV
                     RVTVHAEGVRNGIAAVRIFAGDSLVSMENIPAGRPTDLRLRHPRPWSPDDPYLYRIQV
                     SLGHDTVRGQFGMRSIAVRRDAAGTLRLFLNGRPLFEYGLLDQGWWPDGLYTAPTEPA
                     RLNDLVTIKSLGFNLLRKHVKVEPARWYYDCDSLGILVWQDMPSADNRTAAERTEFRK
                     ELSRVVDALDTHPSIVMWVPFNEGWGQWNPAATREVTAWLKRRDPSRLVDDASGWTDA
                     GVGDVHDIHTYPGPDLPVPDPHRALVVGEFGGLGLPLAGHTWQDQGSWGYRRFGTLDS
                     LGDAYTALIEQLRRLEGAGVAAAVYTQLSDVEVEVNGIMTYDRAVVKLPASAPHLHQL
                     LVHAPPTVRPLLPAGPAGVWRYTTDTPDSGWAGPGFDDHGWRVGHAPFGDSADIPVAT
                     RWSTSDIWLRRPFTLADTSWGTPYLRLAHDDAADVYLNGSLVGHYDAWTSSYRLFPFD
                     VARAGLHAGANLVAVHVHQDRGGQVFDLGIDAVTDTAAPRISAAPFGRTPDGQDVTAY
                     TLDNGRGMTVRAMTYGATILSIRVPDAHGAIGDVTLGFDSLAPYLTESPYFGAVVGRY
                     ANRIARGRFTLDGREYQLAINNAPNALHGGLRGFDKVVWHAVPFRHGDSVGVAFTYVS
                     PDGDQGYPGTLTVHVTYTLTPDDRLAVDYDATTDRATVLNLSQHTYFNLAGEGSGDVL
                     AQELTLHASHYTPVDSTLIPTGVIAPVAGTPFDFRSATAIGSRIALPDTQLAYGHGYD
                     HNFVLDRDGPGLVPAARAVDPVSGRTLEISTDQPGIQFYTGNFLDGTIHGKAGHVYVH
                     RGAFCLETQHFPDSPNHPGFPSTVLRPGERFTSRTVYRFGTTTVSGH"
     gene            complement(13435..13848)
                     /locus_tag="VFU45_03835"
     CDS             complement(13435..13848)
                     /locus_tag="VFU45_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEU5358230.1"
                     /translation="MSANDPDRVRLDKWLWAARFYKTRALAAEAIDGGKVHVNGERAK
                     RSKLLQLGDDVRLRQGTYEYRLTVRGMSERRGPAKVAVTLYEEDAASRAARERMAEQL
                     AAMPAPFQVEKGRPTKKDRRELERFRRSMDGDDEE"
     gene            complement(14038..14976)
                     /locus_tag="VFU45_03840"
     CDS             complement(14038..14976)
                     /locus_tag="VFU45_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEU5358231.1"
                     /translation="MPVSLPYVSELQRLPGWRRMRTRFAPSVTGFLHLGHLVNAVYVW
                     GIARAIGGEVALRLEDHDRGRFRPEYEAAILDDLDWLGLTPDVGTTAEFRAGSSPWRQ
                     SDGSVRYEAALEKLRAMGLTYACECSRSTLARAGGRDDGEEERRYPGTCRDKGLTEDA
                     GRRIRVRMEPGVERFDDLRLGPQEQDPSAQCGDLVARDVQGDWTYQFAVAVDDYEQDI
                     SLVIRGLDLLASTGRQLRLARLLGRPEPPRYLHHPLVTDAAGRKLSKRDFARSIADHR
                     AEGRTPESLLGEAAFLGGLRPEARPLGAAELPGLFG"
     gene            15083..15673
                     /locus_tag="VFU45_03845"
     CDS             15083..15673
                     /locus_tag="VFU45_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU5358232.1"
                     /translation="MDLRQRLLDAAARLYAEAGYRGATTRRIAQEAGVNELTLFRHFG
                     SKAELLHTAIGSCQADAGDLGLPDVPGDPVAEIAEWASRRVADIRRHGALIRTALGEV
                     EEHPDLLRPSEHPMTMAGARLAGYLERLQRSGAAGGEFDPRTAATMFLSVLFVDAIVR
                     DVMPGLFGRDPGRDLDEYVRIFLRGIGVEIRETDRG"
     gene            15670..>16961
                     /locus_tag="VFU45_03850"
     CDS             15670..>16961
                     /locus_tag="VFU45_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5358233.1"
                     /translation="MNGLWRSAAAGMLLAGVAGGAVAQAPVRRISLDTAVTLSERSNE
                     TIGIARAGVDRAGAELSRTRSAFLPQLTGVASYTRTLESQFSALGGGADSGASTSCAK
                     FVPNTGLPIDQRVDSLEAAVQCQSGSNPFSAFKNLPFGRPNEYDVGLQGSWVLFNGLQ
                     NVGDRAAAAAGKRSAETELTAQQAQNIVTTAQAYFDALLADRLYVIADSALVQAERTL
                     SETKLAAQVGSKAEFDLLRAQVARDNQVPVLIQRRAARELAYLRLRQLLDIPAGDSLE
                     LTTPLEGPEAALPPQVSRFSGDTAVDDRAAVVEASATVDQMAARRRSARGTMWPSLTL
                     SSTYTKLAYPSSGLPDLNSFVTNWNVVLGASVPLFTGGRLGAQRRTAEANLDEAQLRL
                     RQARKAAALDARSAETQLEAAEAAWTAGQGTVEQASKA"
BASE COUNT         2540 a         6019 c         5867 g         2535 t
ORIGIN      
        1 actcgagcct ggagagacgc tcctcgtcgg cgcaccgacg ccggtccccg ggccgcggaa
       61 gcgtgcgtcg cggccccggg ggcatcagtg aggggcgagc cgtgggcgtc gggccctgcc
      121 atcccttgac accttcccgc cagccagtag cttgaggaaa cgccgtaacc cgttgtcatt
      181 cttcactctc cacctctttc cgggcctatg gcgaccgctg cagccggcaa tgaacgcctg
      241 ggcgacatcc tcgtccggga aggcctcatc aatcgggacc agctgagcca ggcactgctc
      301 gagcagaaga gctcggggca ccgcctgggc tacgtgctgg tcaagctcgg gctcgtgcag
      361 gagctcgagg tcaccaaggt cctggccaag cagtaccgga tgccggcggt ggacctctcc
      421 cggttcgagg tcgatcccaa gatcatcaag ctggtcccgg cggacctggc gctcaagaag
      481 tcggtgctcc cgctcaagcg ggaagggcgg acgctcaccg tggcgatggc cgacccgacc
      541 gactccggcc tgatcgagga cctcaagttc atcacccggt acgacctctt cccggtgatc
      601 gccggggagt acacgctccg gagccttatc gagaagcact acaactcggg cgaccagcag
      661 ctggccgacc tgctgaagga gatggagggc gagggggaga ccgacgacgt cgaggtggtg
      721 aaggaggagg aggacgagca ggcgtcgcag gcgcagatcg acgacgcgcc ggtggtgaag
      781 ctcatcaaca ccatcctcac cgaggcggtc aagcgcgggg ccagcgacat ccacatcgag
      841 ccgttcgagc acgagatccg ggtgcgcttc cggatcgacg gcgccctgat ggagatcatg
      901 aagccgccga tcaagatgaa ggcggcgctc acgtcgcggg tcaagatcct gtcccagctc
      961 aacatcgccg agcggcgggt gccgcaggac ggccggctcc ggctcaagat gggcgcccgg
     1021 gtcatcgact tccgggtgtc gacggtgccg gtgctcttcg gggagaagat cgtgctccgg
     1081 atcctcgaca aggggaacct gacgctcgac ctctccaagt tcgggttcga gccgaaggcc
     1141 gagcaggacc tgatgcggaa catcatgaac ccgtacggga tggtgctcgt caccggcccg
     1201 acggggtcgg ggaagaccac cacgctctac tcggcgctgt cgcaggtcaa caaggtcgac
     1261 gtgaacatca tgaccgccga ggaccccgtc gagtacaacc tgatggggat caaccaggtg
     1321 ctggtgcgga acgaggtggg gctcacgttc gcggcggcgc tcaaggcgtt cctccggcag
     1381 gacccgaaca tcatcatgat cggcgagatc cgcgacctcg agaccggcgg catcgcgatc
     1441 aaggccgcgc tcacggggca tctggtgctc tcgacgctgc acaccaacga tgcgccgtcg
     1501 accatcaccc gcatgatcga catgggcatc gaggcgttca acgtggcgag cgcggtgaac
     1561 ctggtggtgg cccagcggct ggtgcggcgg atctgcaaga actgcaaggc gccggccaag
     1621 tacaagccgg aggagatcgc gacgctgatc ccgaacccgg aggacgcggc ggggatcgag
     1681 ttcatgaagg gcaccggctg cgacacctgc aacggcagcg gctacaaggg ccgcgcgggc
     1741 ctctatgagg tgatggcgct ctcgcccgag ctccggcggc tcatcctccg gggcgcgtcg
     1801 gtggtggagc tccgcgacca gggggtggcg gaggggatgc tgacgctccg gatggacggg
     1861 atcgtgaagg tcaagaaggg cgtcaccaca ctcgaagaag tgatcaagga gacggcggga
     1921 tgacggtcaa cctgcggagc ctgctcgagg agatgatcga gcgcgaggcc tccgacctgc
     1981 acatcaccgc cggcgagcgg cccaagctgc gcgtcgacgg cgacatcgtg aactcctcgt
     2041 gcgaggaggt cctgacgccg aaggacacgc tctcgctggc gtactcggtg ctgaccgaga
     2101 accagaagaa gcgcttcgag aacgacaacg agctcgactt ctccttcggg atccagaacc
     2161 tggcgcgctt ccgcggcaac gtgttcaagc agcgcggctg cgtggcggtg gtggtgcgga
     2221 tgatcccgtt cagcgtccag acgttccagg agctcgggct ctcgccgatc atcgccaagc
     2281 tggcggagcg gccgcgcggg ctggtgctgg tcaccgggcc gaccggctcg ggcaagagca
     2341 ccacgctcgc ggcgatgatc gacaagatca acaaggagcg gaagggccac atcatcacgg
     2401 tggaggaccc gatcgagttc atccaccggc accagcagtg catcatcaac cagcgcgagg
     2461 tgggcaccga cacgaagagc ttctcgtcgg cgctcaagta cgcgctgcgg gaggacccgg
     2521 acgtcatcct catcggtgag atgcgggacc tcgacaccat cggggcggcg ctcaccatcg
     2581 ccgagacggg ccacctcgcg ttcgcgacgc tgcacaccaa ctccgccgcc gaggccatca
     2641 accgtatcat cgacgtgttc ccgtcgaacc agcagtcgca ggtgcgggcc cagctggcgt
     2701 tcgtgctgga ggggatcatc acccagaccc tgctgcagaa gtccaagggc aagggccggg
     2761 tcatggcggc cgagatcatg gtcgcgacgc ccgccatccg cgcgctcatc cgcgacgaca
     2821 agatccacca gatctactcg gccatgcagt cgggcaagaa gcacggcatg cagacgatga
     2881 acgacgcgct gtaccagcac tacaccaacc gcgacatcac gctcgaggag gcgctgcggg
     2941 tgacgcacga ccccgtcgag ttccagcgga tggtcgggat cacgccggcc gaagaggact
     3001 tcgggacggc cgacaagcca cagcggccgg cgctcaagcg ctagcccgga agggagcttc
     3061 ggatgccgat cttcgagtac tcggcccgca atccgcagaa caaccagatc ctcaagggga
     3121 ccatcgacgc ccccaacaag gacgaggcgg ccaagcagct gcgccagaag aagctgctgc
     3181 tggtcaacct gcgggagcag cccaagcagc tcaagctcaa cttcggcggc ggcgggatcc
     3241 ccacgcgcga cgtggtcatc tttgcccggc agttcgccac gatgatcaac gccggcctgc
     3301 cgctggtcca gtcgctcacg gtgctctccg agcagacgga gaacaaggcg ctgggccagg
     3361 tggcgcgcga ggtggtctac gacgtcgaga gcggcaacac cctcgctgac gcgatggcca
     3421 agcaccccaa ggcgttcagc gagctcttcg tcaacatggt ggccgccggc gaggcgggcg
     3481 gcatcctgga caccatcctg ggccggctcg ccggcttcct cgagaagaac gacgccctcg
     3541 tccggaaggt gaagggcgcc atggtctacc cggtggtcat catgtcggtg gcggccatcg
     3601 ccatctgcgt gctgctcatc ttcgtcatcc cgacgttcca gacgatgttc gcctcggtca
     3661 acatggagct gccgctcccg acccggatcg tgatcggcgc ctcgcacatc ctgctcggct
     3721 tctggtgggc catcatcctg gcggtcggcg gcacggtctt cggcatcaag aagtactaca
     3781 ccacggccga cggcaagaag cggatcgaca cgatcatgct ccagctgccc atcctgggcg
     3841 acctgatccg gaagtcggcg gtgtcgcgct tcacccggac gctcggcacc ctcctctcct
     3901 cgggcgtgtc gatcctcgac gggctcgaga tcacggccaa gaccgccggc aaccgcgtca
     3961 tccacgacgc cgtgatggcc tcccggcagt cgatcgccgg cggtgagacg atctcggcgc
     4021 cgctcgcgaa gtcgaaggtg ttcccgccga tggtggtctc gatgatcgcc gtcggcgagc
     4081 agaccggcgg cctcgatgag atgctctcga agatcgccga cttctatgac gaagaggtcg
     4141 acgtcgcggt cggcgccctc ctgtcgctga tggaaccggt catgatcgtg atcctcggcg
     4201 tggtcgtcgg cggcatggtc gtcgccatgt acctgccgat cttcgggatg atcaacacgg
     4261 tgcagtagcc ccgcgccgga gcaccccacg gtccggctct tcgccgggcc gtgccgagcc
     4321 cccgcctgcc ccggcgcggg gctcgttccg tccgaggaga gaggaacgcc cgccgatgat
     4381 ccagctccgc cgcacgctgg tgccggccgc tgcaggcctt cgtgccttct ggccgacggc
     4441 catgctggtc gtggctaccg ccacggtcgc gctcatggct ctcctcccgg tcgcgagcct
     4501 ttctggtgcc acatccgtct tcctccctcg gctttccctc cccgcagcgc cggccataga
     4561 tctcggcatt cgctggagca gtgtggcaca gggtccctcc gacctgcatg ccctggggat
     4621 cacggcgctc ttccggctgt tgctcggcgt cgccgccgca ctggccggcg tggcactcct
     4681 ggccatctcc gccgttgccg ctgcccgggc ccagcaacgc cagggcgagg tgtcgatcca
     4741 ccggtcggtc ggcgcatctc gccggcactt ggtgctctcc catggtgccg agggcgtcgt
     4801 ggtctccctg ctggggctcg gcgtgggcgc ggtcctcggg cgcaccatcg cggtgtgcgc
     4861 agcgcgatac tggcccggtc acctcgcgcc gaccggcccg ctccccggga tcagcgccgc
     4921 cgctgcgctc gcgggggcga tggtccttgg cgccctcttc ccgctggtct acgcccggcg
     4981 ccgtgtgccg cgcaccgccg tcggtggcga gccactcgga ctggccatcc cgacgttcca
     5041 gctggggctc tccctgacgg tcctggtcgc cgcggtactg ctctggcgcg gggccggcgg
     5101 ctccgcccgg agaggtccat cggataccga cctgaccgtc atacacctcg ctgccctcac
     5161 ggggtccgcc gctgccgccc ggtcagccca gtatgcgggt gtactcgcgc acgtgagaag
     5221 tgccgcaggg gtccgcatcg ccgccctcac cagccccggc acggtgtcgg gcgcggggga
     5281 gtcggatatc gccgtggtgc attgcgggga cgggtgctcc ctcggagggc tcccgctgcc
     5341 gtaccagacg gctcgggcga tgcactacgc cgtgagcccc gattccttca acgcgatggg
     5401 attgccgatc atcgcggggc gtggcatcgc cgacactgac acttggcggg cgccgcgggt
     5461 ggcggtcgtg aatcaggcct tcgccatcgc gcacttcgag cgcggtgatc cgctgggcca
     5521 cgaactgcgg ctcggcgccg gtccctggtc gcggttcgtc gtcgttggcg tagtggccga
     5581 tcagccggtc gaaggggtcg gtggaggggc ggcgccagcg aatgcgatct acgtgagcgt
     5641 gctccagcaa ccgccgccga acgtcgatct gctcgtccgc ggcggcgtgc ccgccgccgt
     5701 cgccggcgcc gcccgcgcgg cgctcggtcc cgcggcgggc ccactccgca cgacctcgaa
     5761 ttcggaaatc cgcgcgacgc agacggcgcc ggtccgctgg ttcgcggccc tcttcggtct
     5821 ggtggggtgc gcattgctca tcgtcgcgac cgccggaacg gccacggtgg tgcatcagtg
     5881 ggtcgcctcg ctggccgtcg aactcggtgt gcgccgggcg gcaggtgcgt cgcgccagca
     5941 ggtcgtcgca ttcgtgctca tccggttgat cgccatggcc ggggtcggtg cgatgatcgg
     6001 tgtgtggctc ggcaccgggg tctggggcac gctgcatcag gcggtgcccg gggtgccagc
     6061 gtgggatgcg cgcaccgtgc tcgaagcggc gggggtgctc gtcggcgcgg caatggccgg
     6121 cggactggcg cccgccgtgc gcgccgcgct ggcgtctccg gcgggcctga tggatagtgc
     6181 gtgacggtcc gtcggtccca gccgctaccg caccggcggc ccgatcggcc cgctccccgc
     6241 gccggtcttc tcgatggcgc tcccgatctc cgtgaggtcg tccgccgtga gctcgagcga
     6301 cgccgcggcg atccacccgt ccacctgcgc cgggctccgc gcccccacga tggcgccggt
     6361 gaccccccgc cacgcgagcg tccaggcgat ggccaccgcg ccgaccccga cgccccggcg
     6421 ccccgccacc cctcgcagcg tctccaccag cgccaggttc cgctccagcg cctccccctg
     6481 gaagttgacc gcgcgtgagc gccagtcgtt cttcggcagc gccgccgccc gctcccgggt
     6541 gaaggagccg ctcagcaacc ccgacagcat cgggctgtac acgatgacgc cggtgcgatg
     6601 gtcggcgcac caggggagtt cccgctccgc cacgtcgcgc cgcacggccg agaagggcgg
     6661 ctgcaatgag tcgacgtgcc ccagcttctc cgccgcggcg agctgatccg ccttgtggtt
     6721 ggaaagcccg gcggcgcgga tcttcccctc ccgcttgagc tcgagcatcg cgccccagta
     6781 ctcctcgagc ggggtcccgt cctccggagg ccagtgaagc tggtagaggt cgatccgctc
     6841 gaccccgagc cgccggagcg acccctcgag ctccttccgg atgctcgccg ggctcgccac
     6901 ccgcgccggt ggcttcgacc ggtcggcctc gtcccatacc attccgcact tggtgaagac
     6961 atacggccgc tccgatgccg gcaggccgcg cagtgcccgg gccaccacat cctctgaatg
     7021 tcccagccca tacaccgccg cggtgtcgat ccagttgatc ccgcgggcca ccgcgtgccg
     7081 gatggcggcc acggactcgg cgtcatcctg gtggccccaa ccgaactgcc agtccccccc
     7141 gccgatggcc caggcgccga gcccgacgcg cgtgatctcc atcccggtgg tgccgagagg
     7201 acgggtggga aggggagcgt gatcggtcat cgtggtgccg ccgtggtgta ttcgggttcg
     7261 acgttctcga agaagagccc ctggaactgc cccgcgcgca tctgtggcac ctcgagctcg
     7321 atgtgatgcg gccggtccgc ggtgagaagc gacacgtcgg cgtagaagcc cacgaaatcg
     7381 tggggcaccg ggcgcaccga cgagtacgcc ttcttcacct cgaccgggcg cccgtcgatc
     7441 cacatcctga gctcccatcg gtcgtccggc tccgcgaact gggcatacag cagcagccgc
     7501 tcgggcacga gccacggcgt ggcatggtcc tccgccgtcc acggcaccgg ccactgccgc
     7561 gcccggcggg ccagctggtc gaacacccgg gccgggaccg cgaacgtggc ctgccacggg
     7621 ccaccggtga aggtcggatc gtatgcgccc acccgcgcat cggcaccgaa cgccgcgccg
     7681 gcgaaccgga ccggcaccgt caccattccc ccggcgatcc gggcatccgc ggccaccgcg
     7741 ccatccacgc ggacggtggt cacccgcgcc ccagcgggga gaaggatcgt ggcgtccgcg
     7801 tcggtgcccg cgtctccacg cattccgtcg agcgccagcg tgtcgccggc cagcctggcg
     7861 gagcccggca cgccgaagag gatcggcgcg tcgatccgcg ctggcgccgg cgccacctgg
     7921 agcacgagcg cggtgaccgg cggcaccgtg acggtcatgg tgtcttgcgc cgaccagatc
     7981 ccgcggtccg ggctggccag gcggcggccg gacaggggct cacgctcggt cacgatccag
     8041 cgcccgcggg cggggaggcc gatggcgccg ccgagcggca ggcttgcgcg ctccgggatc
     8101 gcgttcgggt tgaagaggaa gatgtatccc gaatcgccat cgacggccga ggtgccgtcg
     8161 accttgccga aacccggcgg acccaggatg gtacgcgtgc ggcgcaggag ttcccgatga
     8221 gcgtcggccc acgtcagcca tccgcggagc cacgcgccgt cctcggcccg gaagagggtg
     8281 tcctcggcca ggtcccgcgc cgggatgtcg ttgatgacat tgttccaccc cgccacggcg
     8341 atcgatgaca ggacggagta gcgccagccg aggtagtccc agtcccgggt gcggaatgac
     8401 gtgagctccc cgccgcgcgc ggtcctccgc tccggcatcc gtccggtggc atccgcacgc
     8461 ggggtctggt gcgtcatgaa accgggtacc agctcgctcg gcgcgaattc gtagttgcgg
     8521 taacggtacg cggtgtagcg ttcgcggtcc gcagagaccc ggtcgatgtg gaggtcgggg
     8581 aaaggcacga agctctccgg ctgctcgtcg gtgccggtgg gatgcggata cgtccccgcg
     8641 agccacgacc agggaccgta gagctggtag gcctggcgcc cgtcgatcac gatgccggga
     8701 ttccggcgct tgagctcctc catgacccgg cgccatcccc accactgggc gtaggtcccc
     8761 gcccccggga ggtcgaggaa ggtgtggtcg aaggaatacc cgccgatccc gttgcggcgg
     8821 tagaacgccg tcaactgggt gataagccag tcctgcagcg cgcggctggc gaggttggcg
     8881 taatgccgat ccggcgcatc gggcggagac acgatccagc tcgaatcggc ggcgaagggc
     8941 atcaccgggt agacgtaggc gaggagcttg agccctcggc tccgggcgta gtcgatcatc
     9001 cccttgatgg aggcggggag ggtgtcgcgg tcggggcgcc attcgccacg ccggatgcgg
     9061 gggccgaggc cgaaccagag cacgtactcc cagtgccagt cgtccacgct ctcgtcgcgg
     9121 gtggacagcg tggagttggc cggcgcgtac agcacgtcgc gggcgccgag ctccgctgcg
     9181 cggtcgagca ccctgcggta ctccgcctgg cctgcggcgg tgccgacgtc gatctggtaa
     9241 tcgttggcgg tccaacccac gaacacgtcc gtcgggtgcg ccggcggggc aatgaggaag
     9301 gcgcgcaccg cgtcggtgaa cgccaagacc tccgcctcgt cgaggccgtc cgccgtgacc
     9361 gcgggatgcc acgcccactc tggcgccatc gcggcgggga gccggcggcc ggtggcctga
     9421 tacgggacga gcagcgcccg gtcggcggga aatgcgccgt ccgctgcgtt ccacggcatt
     9481 gcgggtgcat aggacaccgt taccagtccg ccggcgcgac cgatcgacag gaaggggtcc
     9541 tggaccagcg ccagcaggcc ggggccccgg tcgcctcgga tggcggcgcc ccacgcaccg
     9601 gtgtcgagtt cgccattcgt ggccgggatc gtatgcacgt cggcgggggc cacctcgaat
     9661 cccagccgga gcggtgtcac gctgtccacc cgccaggccc ccgagcccgt gaccgtgagc
     9721 tgcttgctca cgaaggccca gtcgggccgc gtctcataga cgacctcgat gacgtacgct
     9781 ccggccttcc agcggtaggt ggtccggccc cccgcggcag agcgggccgg cgcggggaga
     9841 tccgcgctgt catatgcctt cccgtcgatg accagccgga agccatcggt gcggaggcgc
     9901 cacgcggccc cgctccggcg gtcgcggatc tccgtcaggc cgcgggggcc gaaagcggcc
     9961 tcgaccgctg accggggcgg aacgggcccg ccagcgccga gcgcggtcgt gagtgcgaac
    10021 agggacggga tgagcgcgat cacgggatct caccgaatga gggatgctgc aagatttcca
    10081 gccgggcgcc gcccggcaac cggtgcgtgg cggcatcacc cggccagccg taagttacgc
    10141 ccatcccctc acccgtgtga ctgcaatgcc gatccgtcga tccgcttccg tgctgcttct
    10201 gctctttgcg ctcgccgcct ccgtcgcgtc ggcccagtcc cggctcgtga cccgttgggc
    10261 cgccgccgtc gacaccctgc atccgctccc cgagtacccg cgccccgaaa tgcgccgcag
    10321 ttcgtggctg gacctgaacg ggccgtggga ctacgccatc caggacggcc acgccgcgcg
    10381 tcccgaacac tgggatgggc ggatcgtggt gccgttcgcg gtcgagtcgc agctctccgg
    10441 cgtgcggcgc ccggtcttgc cgagccaggc gctctggtac cggcggcggg tcgaggtccg
    10501 gcccgccgcg ggcacgcgct ggctcctgca cttcggtgcg gtggactgga gcaccaccgt
    10561 ctgggtcaac ggcaaggtcg tcgggcggca cgtcggcggc tacgacccat tcacgttcga
    10621 tgtgaccgcc gcactcgctc catcgggcag ccaggagatc gtcgtacagg tgaccgaccc
    10681 gacggattcc ggcggtcagc cgcgcgggaa gcaggtgctc aagcccggca gcatctggta
    10741 cacggcagtg acgggcatct ggcagactgt ctggctcgag ccggtgccgg tggcgcacat
    10801 cgaacggctc ggcatcgttc ctgatctcgc cgacggccgc gtgcgggtga ccgtccacgc
    10861 cgagggcgtc cggaacggga tcgccgcggt ccgtatcttc gcaggcgaca gcctggtctc
    10921 gatggagaac atcccggccg ggcgtcccac tgacctccgg ctccggcatc cccgcccctg
    10981 gtcgccggac gatccctacc tgtaccggat ccaggtatcg ctcggccatg acactgtgcg
    11041 cggccagttc gggatgcgga gcatcgccgt gcggcgcgac gcggcgggga cgctccgcct
    11101 cttcctcaac gggcgccccc tcttcgagta cggcctcctc gaccagggct ggtggcccga
    11161 cgggctgtac actgcgccca ccgagccggc ccggctcaac gacctcgtca ccatcaagtc
    11221 gctcggtttc aacctcctcc ggaagcacgt caaggtggag ccggcgcgct ggtactacga
    11281 ctgtgacagc ctcggcatcc tggtctggca ggacatgccg agtgcggaca atcgcaccgc
    11341 ggcggaacgg accgagttcc ggaaggagct ctcccgcgtg gtggacgcgc tcgacaccca
    11401 tccgtcgatc gtgatgtggg tgccgttcaa tgagggctgg ggtcagtgga acccggcggc
    11461 gacgcgcgag gtgacggcgt ggctcaagcg ccgggatccc tcgcgcctgg tcgacgacgc
    11521 cagcggatgg accgacgccg gtgtgggcga cgtgcatgac atccacacct atcccggccc
    11581 cgaccttccg gtgcccgacc cgcatcgcgc gctggtggtg ggggagttcg gcgggctcgg
    11641 cctgccgctc gccgggcaca cctggcagga tcagggaagc tggggctatc gccgcttcgg
    11701 gaccctcgac tccctgggcg atgcgtacac ggcgctcatc gaacagctgc gccggctgga
    11761 aggggccggg gtcgcggcgg cggtgtacac ccagctctcg gacgtcgagg tcgaggtcaa
    11821 cggcatcatg acctacgatc gcgcggtggt gaagctgccg gcctccgcgc cgcacctgca
    11881 ccagctcctc gtgcacgcgc ccccgacggt gcggccgctc ttgcctgcag ggccggcagg
    11941 ggtgtggcgc tacaccactg acacgcccga cagcggatgg gcgggtcccg gattcgatga
    12001 tcatggttgg cgggtcggcc acgccccgtt cggcgacagc gccgacatcc cggtcgccac
    12061 ccgttggagc acctccgaca tctggctccg ccggccgttc accctggccg acacgagctg
    12121 gggcacgccc tatctgcgcc tggcgcatga cgatgcggcg gacgtctacc tcaacgggag
    12181 cctcgttggc cactatgatg cgtggacctc gtcgtaccgg ctcttcccct tcgatgtcgc
    12241 ccgggcgggc ctgcacgcgg gcgccaacct tgtcgccgtg cacgtgcacc aggatcgcgg
    12301 ggggcaggtc ttcgacctcg ggatcgacgc cgtgaccgac accgccgccc cacgcatcag
    12361 tgccgccccg ttcgggagga cgccggacgg ccaggacgtg accgcgtaca cgctcgacaa
    12421 cggccggggg atgaccgtcc gggccatgac ctacggcgcc accatcctgt cgatccgggt
    12481 gcccgatgcg cacggcgcca tcggcgacgt gaccctcggg ttcgactcgc tcgcgcccta
    12541 cctcaccgag tcgccgtact tcggcgccgt ggtgggccgc tacgccaatc gcatcgcgcg
    12601 cgggcggttc acgctcgacg gccgcgagta ccagctggcc atcaacaacg cgcccaacgc
    12661 gctgcacggc ggcctgcggg ggttcgacaa ggtggtgtgg cacgcggtgc cgttccggca
    12721 cggcgactcg gtgggtgtcg cgttcacgta cgtaagcccc gacggcgacc agggataccc
    12781 gggtaccctg accgtgcacg tcacgtacac gctcacgccc gatgaccggc tggccgtgga
    12841 ttacgacgcg acgaccgacc gggccaccgt cctcaacctg agccagcaca cctacttcaa
    12901 cctggcgggc gaggggagcg gcgacgtgct ggcgcaggag ctcacgctcc acgcctcaca
    12961 ctacacgccg gtggactcga ccctcatccc gaccggggtc atcgcgccgg tggccgggac
    13021 gccgttcgac ttccggagcg ccacggcgat cggctcccgg atcgcacttc ccgacaccca
    13081 actggcgtac ggccacggct acgaccacaa cttcgtcctc gaccgcgacg ggccggggct
    13141 cgtgcctgcc gcccgcgcgg tggacccggt gagcgggcga acgctcgaga tctcgaccga
    13201 ccagcccggg atccagttct acaccggcaa cttcctcgac ggcacgatcc acgggaaggc
    13261 aggccacgtg tacgtccatc gcggcgcgtt ctgcctggag acgcagcatt tcccggattc
    13321 gccgaaccat cctggcttcc cgtccaccgt gctccgcccg ggcgaacgct tcacgtcgcg
    13381 cacggtgtac cgcttcggta cgacgaccgt gtcgggacac tgaaaggcac ccgctcactc
    13441 ctcgtcgtcg ccgtccatcg accgccggaa gcgctccagc tcccggcggt ccttcttggt
    13501 ggggcggccc ttctccacct ggaacggcgc cggcatcgcg gcgagctgct ccgccatccg
    13561 ctcccgcgcc gccctgctgg cggcatcttc ctcatagagc gtcaccgcca ccttggcggg
    13621 gccccgccgt tccgacatcc cgcgcacggt cagccggtat tcatacgtcc cctgccggag
    13681 ccgcacgtcg tctcccagct gcaggagctt ggagcgcttg gcgcgctcgc cgttgacgtg
    13741 gaccttgccg ccgtcgatcg cctcggcggc gagcgcccgg gtcttgtaga accgcgccgc
    13801 ccagagccac ttgtcgaggc gcacgcggtc ggggtcattc gcgctcattt gacctcattc
    13861 gaaaggtggg accacggagg gcacggaggg actcggagcc gcacggagga acagctgaag
    13921 ttgtgggatc gcgcacgagg ctccgctcgc aaggccacct ctacggcagt tctccgtgca
    13981 tctccgtgat gctccgtgcc cgagcctgcc ctgagcgctg cgaagggtgg tcccacatca
    14041 accgaacaga ccgggaagct ctgccgcccc gagcggccgc gcctccggcc gcagcccgcc
    14101 caggaacgcc gcctcgccga ggagcgactc gggcgtcctg ccctcggcgc gatggtcggc
    14161 gatcgacctg gcgaagtcgc gcttgctgag cttccgaccc gccgcgtcgg tcaccagcgg
    14221 atggtgcaag tagcggggcg gctccggtcg gccgagcagg cgggccaggc ggagctggcg
    14281 tcccgtcgag gccagcaggt cgaggccccg gatcacgagc gagatatcct gctcgtagtc
    14341 gtcgacggcc accgcgaact ggtaggtcca gtccccctgg acgtcgcgtg ccaccagatc
    14401 gccgcactgg gcggacggat cctgctcctg cgggccgagt cggaggtcgt cgaaccgctc
    14461 cacgcccggc tccatccgga cccggatccg ccggccagcg tcctccgtga gtcccttgtc
    14521 gcggcaggtg ccgggatatc gccgctcctc ctcgccgtcg tcccgccccc cggcccgtgc
    14581 gagcgttgac cggctgcact cgcaggcgta ggtgaggccc attgcgcgga gcttttcgag
    14641 cgccgcttcg tatcgcaccg agccgtcgct ctggcgccaa ggcgatgacc cggcgcgaaa
    14701 ctccgccgtg gtcccgacgt ccggtgtgag cccgagccag tcgaggtcgt ccagtatcgc
    14761 ggcctcgtat tcgggacgga atcggccccg gtcgtggtcc tcgagccgga gcgccacctc
    14821 gccgccgatg gcccgggcga tcccccacac gtacacggcg ttcaccaggt gccccagatg
    14881 caggaagccg gtgacgctcg gcgcgaagcg ggtccgcatc cggcgccacc cgggcaggcg
    14941 ctggagttcc gagacatatg gtaaggagac gggcacggcc gatagtacga ggggccgggg
    15001 gttctgcaaa gccggaccag cgtcgcggcc aattgtaagc agttgcttgc atctaaatga
    15061 ccatttgttt atttatcgcg ccatggatct ccggcaacgt ctcctcgatg cagctgcccg
    15121 gctttacgcc gaggcggggt atcgtggcgc gacgacccgc cgtatcgcgc aggaggcggg
    15181 ggtcaacgag ctcacgctct tccggcattt cggttcgaag gcggagctcc tccacaccgc
    15241 catcgggtcc tgccaggccg atgccggcga cctggggctc cccgacgttc ccggcgatcc
    15301 ggtcgccgag atcgcggagt gggccagccg ccgtgtggcc gacatccggc gccacggcgc
    15361 gctcatccgc accgcgctcg gcgaggtcga ggagcatccc gacctcctcc ggccctcgga
    15421 gcacccgatg acgatggccg gcgcgcgcct ggccgggtac ctcgagcgcc tgcaacggag
    15481 cggcgcggca ggcggcgagt tcgatccgcg gaccgccgcg acgatgttcc tgtcggtgct
    15541 cttcgtggac gcgatcgtgc gggacgtgat gcccgggctc ttcggccggg atccggggcg
    15601 ggacctcgac gaatatgtgc ggatcttcct gcgcgggatc ggcgtggaga tccgggaaac
    15661 ggacagggga tgaacggtct gtggcggtcg gcggcggccg ggatgctgct cgcgggagtg
    15721 gccggtgggg cggtggcgca ggctcccgtg cgacggatct cgctcgacac ggcggtgacg
    15781 ctttcggagc ggtccaacga gaccatcggc atcgcgcggg cgggggtgga ccgcgcgggc
    15841 gccgagctct cgcggacgcg gagcgccttc cttccgcagc tcaccggcgt ggcgagctac
    15901 acccggacgc tggagagcca gttctcggcg cttggcggcg gcgcggacag cggcgcgtcg
    15961 accagctgcg ccaagttcgt cccgaacacg gggctcccca tcgaccagcg ggtcgactcg
    16021 ctggaggcgg cggtgcagtg ccagagcggc tccaatccgt tctcggcctt caagaacctc
    16081 cccttcggcc ggcccaacga gtacgacgtc ggcctgcagg ggagctgggt gctcttcaac
    16141 ggtctgcaga acgtgggcga ccgcgccgcg gcggcggcgg gaaagcgttc ggccgagacc
    16201 gagctcaccg cccagcaggc gcagaacatc gtgacgacgg cgcaggccta cttcgacgcg
    16261 ctcctcgccg accggttgta cgtgatcgcc gattccgcgc tggtgcaggc cgagcgcacc
    16321 ctgagcgaga ccaagctcgc cgcgcaggtc ggcagcaagg cggaattcga cctcctgcgc
    16381 gcccaggtgg cgcgggacaa ccaggtcccg gtgctgatcc agcggcgcgc ggcgcgggag
    16441 ctcgcgtacc tccggctccg gcagctcctc gacatcccgg ccggcgactc gctcgagctc
    16501 acgaccccgc tcgagggccc cgaggcggca ttgccgccgc aggtgtcccg cttctcgggt
    16561 gacacggcgg tggacgaccg cgccgccgtg gtcgaggcct ccgccacggt ggaccagatg
    16621 gcggcacgcc gccggtcggc acgggggacg atgtggccct cgctgacgct cagcagcacg
    16681 tataccaagc tggcctatcc ctcgtcgggc ctccccgacc tcaacagctt cgtgaccaac
    16741 tggaacgtgg tgctcggcgc gtcggtgccg ctcttcaccg gcgggcggct cggcgcccag
    16801 cgccgcaccg cggaggccaa cctcgacgag gcccagctcc ggctgcggca ggcccggaag
    16861 gccgccgcgc tcgatgcgcg ttcggcggag acccagctcg aggcggcgga ggcggcgtgg
    16921 accgccggcc agggcacggt ggagcaggcg tccaaggcgt a
//
LOCUS       DASUAP010000157         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_903767, whole genome shotgun sequence.
ACCESSION   DASUAP010000157 DASUAP010000000
VERSION     DASUAP010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903767"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFU45_03855"
     CDS             complement(<1..237)
                     /locus_tag="VFU45_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEU5358234.1"
                     /translation="MIAHFWLAALAAFLAATLAAVTGFGGAAVLLPVLVAIVGVRAAI
                     PILTVAQLIGNGSRVWFNRTELDWRLVGWFAIGAV"
     gene            complement(314..386)
                     /locus_tag="VFU45_03860"
     tRNA            complement(314..386)
                     /locus_tag="VFU45_03860"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(351..353),aa:Lys,seq:ctt)
     gene            complement(398..470)
                     /locus_tag="VFU45_03865"
     tRNA            complement(398..470)
                     /locus_tag="VFU45_03865"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Glu,seq:ttc)
     gene            complement(481..553)
                     /locus_tag="VFU45_03870"
     tRNA            complement(481..553)
                     /locus_tag="VFU45_03870"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Glu,seq:ctc)
     gene            complement(574..646)
                     /locus_tag="VFU45_03875"
     tRNA            complement(574..646)
                     /locus_tag="VFU45_03875"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(611..613),aa:Trp,seq:cca)
     gene            complement(807..880)
                     /locus_tag="VFU45_03880"
     tRNA            complement(807..880)
                     /locus_tag="VFU45_03880"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(844..846),aa:His,seq:gtg)
     gene            complement(947..1822)
                     /locus_tag="VFU45_03885"
     CDS             complement(947..1822)
                     /locus_tag="VFU45_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5358235.1"
                     /translation="MRSLGRARLEIAGAALLFSTGGAVIKATTFSGWQVAGLRSAVAA
                     VAVLLLLPASRRGWSWRTLVVAVAYASCLILYVTANKLTTAANTIFLQSTGPIYILLL
                     APWLLREKVSREDLLVLAVMVVGLLCFFAGAEPPLRSAPDPATGNILAALAGVAWAFV
                     VMGLRWLGRSAGPEVADPAMSAVALGNLLAALVALPFGWPLGHGGPADWAAIAFLGIV
                     QIGIAYLLMSRAVRVLPAFEASVLLLLEPALNPFWAWLVQGEVPGAWALAGGGLILGA
                     SAAKSWWDSRVPVAA"
     gene            complement(1819..3411)
                     /locus_tag="VFU45_03890"
     CDS             complement(1819..3411)
                     /locus_tag="VFU45_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU5358236.1"
                     /translation="MLLTLALLCQDPLAVRADTVRARHDAVHYDIAAAIGDTGRRFAA
                     EVTTRWLLSSTAPIVVALDTPFAVVGVWVGDSGGRQLHRVTWERDGGLIVIPHTGGAG
                     DSAVTRIRYHGTPADGLIIRRAADGTFTAFADNWPDRAHRWFPSQDVPGDKATASFRV
                     DAPKGYQVVANGRLVGVDTLKDGGTVWRYSQDAPVSPYNLVFGLARFAVTRLGRAGCA
                     VACVPMSVWTYPVDSAYAVTGPFRRAGAILDFLQRTIGPFPYAKLAHVESSTRFGGME
                     NASSIFYDENAYRSQRMREETVAHESAHQWFGDAVTEADWHHLWLSEGFATYFAALWV
                     GHADGDSAGRADWARSAQAIFKSPATERPIIDTAATDLMGLLNTNNYQKGAWVLHSLQ
                     RLVGDSAFFAGIRAYYARFRDRTALSSDFAAVMGQAAGTDLRWYFDQALLQPGYPKLE
                     VRSAFDSVARRLTLTVRQVQPAGWGTFRLPGLAFDIDGVVSRHDIAGPETVIAFDHVD
                     HAPAAVTVDPDGDWLLQATVVK"
     gene            3478..3990
                     /locus_tag="VFU45_03895"
     CDS             3478..3990
                     /locus_tag="VFU45_03895"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HEU5358237.1"
                     /translation="MRRLAGLAALAILAAPLAAQEPPPRIDRSWLRIDRGARRVTFTL
                     LAGATPTNGGLNFNGFTAGRLTLEVPGGWEVVVRHRNLDPKLPHSVELIGDRDPLPVQ
                     AVPPVVPGAASARLLEGIPPGGVETIRFTAPDGEYRLFCAVPGHGLAGMWLRVSVSTR
                     VRTPALRATP"
BASE COUNT          677 a         1414 c         1444 g          545 t
ORIGIN      
        1 cactgccccg atggcgaacc atccgaccag ccgccagtcg agttcggtgc ggttgaacca
       61 gactcggctc ccgttcccga tgagttgggc caccgtgagg atcgggatgg cggcgcggac
      121 gccgacgatg gcgacgagca ccgggaggag gaccgcggcg ccgccgaacc ccgtcaccgc
      181 ggcgagggtg gccgccagga atgcggcgag ggcggcgagc cagaagtggg cgatcacccg
      241 agaaaactaa ctccggcgat gatggcacga acgacgaacg cccgctcacg tgagcaggcg
      301 tcggtcgatc atatgcgccg cgcaggaatc gaacctgcaa cctagtgatt aagagtcact
      361 tgctctgcca attgagctag cggcgcgtca gttgtgatag ccccaacggg agtcgaaccc
      421 gtctctcgac cttgaaaggg tcgcgtcctg aaccgataga cgatggggcc gactccttca
      481 tagcgctaac gggattcgaa cccgtgtttg ggccttgaga gggccccgtc ctagtcccct
      541 agacgatagc gccatcgact tcgtccatcg cgacaggccc ggcaggaatc gaacctgcaa
      601 cccccggttt tggagaccgg tgctctacca attgagctac ggacctctgg ggagagtcta
      661 gagcttagag cttagagtct agagcttgga gcctagagtc tagagcttgg agctttctcc
      721 agccgaactc cgagctctcg acctttagct ctaagctctc agctctcggc tctcggctct
      781 cagctctcag ctctccactc ccacagggtg gctgacgggg atcgaacccg caacctccgg
      841 tgccacagac cggcgctcta accgattgag ctacaaccac cacgggcact tccgggccgc
      901 caaaactagt gaaacgaccc gtgtcaggca acctcccgag aatgcgtcac gcggccacgg
      961 ggacgcggga atcccaccag ctctttgcgg cgctcgcgcc caggatcagc cccccacccg
     1021 ccagcgccca agccccgggg acctccccct gcaccagcca cgcccagaag gggttgagcg
     1081 cgggctccag gaggagcagc accgatgcct cgaaggcggg gagcacccgc acggcgcggc
     1141 tcatgagcag gtacgcgatg ccgatctgca cgatgcccag gaacgcgatc gccgcccagt
     1201 cggcgggtcc gccgtgcccc aggggccagc cgaacgggag cgccaccagc gcggccagga
     1261 ggttgcccag cgccacggcg ctcattgccg ggtcggcgac ctcagggccc gcggaccggc
     1321 cgagccagcg gagccccatc accacgaagg cccacgccac tcccgccagc gcggcgagga
     1381 tgttcccggt cgccggatcg ggagccgacc ggagcggcgg ctcggcgccg gcgaagaagc
     1441 agagcaggcc gaccaccatc acggccagga ccaggaggtc ctcccgcgag accttctccc
     1501 gcagaagcca cggcgccagc aggaggatgt agatcggccc ggtggactgg aggaagatgg
     1561 tgttggcggc ggtggtgagc ttgttggcgg tgacgtagag gatgagacac gacgcatagg
     1621 ccaccgcgac caccagcgtc cgccagctcc acccgcgacg ggaggccggc aggaggagca
     1681 gcacggccac cgccgccacc gccgagcgga ggcccgccac ctgccagccg gagaacgtcg
     1741 tcgccttgat gaccgcgccg ccggtcgaga agaggagcgc cgcgccggcg atctcgagcc
     1801 gggcccggcc cagcgacctc acttgacgac cgtggcctgg aggagccagt caccgtcggg
     1861 gtcgacggtg acggccgcag gggcatggtc gacgtggtcg aaggcgatga ccgtctcagg
     1921 tccggcgatg tcgtggcggg agacgacgcc atcgatgtcg aacgcgaggc ccggcagccg
     1981 gaaggtgccc caccctgcgg gctggacctg tcgcaccgtg agcgtaaggc ggcgcgccac
     2041 cgagtcgaag gcgctccgga cctcgagctt cgggtatccg ggctgcagca aggcctggtc
     2101 gaagtaccag cgcaggtcgg tgccggccgc ctggcccatc accgcggcga agtcgctcga
     2161 gagcgcggtg cggtcgcgga aacgcgcata gtaggcgcgg atcccggcga agaatgcgga
     2221 atctccgacc aggcgctgca gtgaatgcag cacccaggcg cccttctggt aattgttggt
     2281 gttgaggagg cccatcaggt cggtggcggc ggtgtcgatg atgggacgct cggtggccgg
     2341 ggacttgaag atggcctggg cggaacgcgc ccagtccgcc cggccggcgg agtcgccgtc
     2401 ggcgtggccc acccagagcg cggcgaagta ggtcgcgaac ccctccgaca gccagagatg
     2461 gtgccagtcg gcctcggtca ccgcgtcgcc gaaccactgg tgcgcgctct cgtgcgccac
     2521 ggtctcctcg cgcatccgct gcgaccggta ggcgttctcg tcgtagaaga tcgacgaagc
     2581 gttctccatc ccgccgaagc gggtgctcga ttcgacgtgg gcgagcttgg cgtaggggaa
     2641 ggggccgatc gtccgctgca ggaagtcgag gatcgcgccc gcccggcgga agggccccgt
     2701 gacggcatac gccgaatcca cggggtaggt ccataccgac atcggcacgc aggcgaccgc
     2761 gcaccccgcc cggcccaacc gggtcacggc gaaccgggcg agcccgaaga ccaggttgta
     2821 gggcgacacc ggcgcatcct gactgtagcg ccacaccgta ccgccgtcct tcagcgtgtc
     2881 gacccccacc agccgcccgt tcgccacgac ctggtacccc ttcggcgcgt cgacgcggaa
     2941 ggaggccgtg gccttgtcgc cggggacgtc ctggctgggg aaccagcggt gggcccggtc
     3001 gggccagttg tccgcgaagg cggtgaaggt gccgtcggcc gcccggcgga tgatgagacc
     3061 gtcggccggc gtgccatgat accggatccg ggtgacggcg gagtcgccgg cgccgccggt
     3121 gtgcgggatc acgatcagtc cgccgtcgcg ctcccacgtc acgcggtgga gctgccggcc
     3181 gccggagtcg ccgacccaga cgccgaccac ggcgaacggg gtgtcgagcg ccacgacgat
     3241 gggagcggtg gaggacagga gccagcgggt ggtgacctcg gcggcgaacc gtcggccggt
     3301 gtcgccgatg gcggcggcga tgtcgtaatg gaccgcgtcg tggcgggccc gcaccgtgtc
     3361 ggcgcgcacc gcgagggggt cctggcagag gagggcgagg gtcaggagca tcggcgcaat
     3421 ctgtcgcggc gccgccgccg atggtagccc gtggcccggc aggattagct tcccacgatg
     3481 cgccgcctcg ccgggctcgc cgcccttgcc atccttgccg cgccgctcgc ggcacaagag
     3541 ccaccgccgc ggatcgaccg ctcgtggctc cggatcgacc gcggggcgcg ccgggtcacg
     3601 ttcacgctgc tcgccggcgc gacgccgacc aacggggggc tcaacttcaa cgggttcacc
     3661 gcgggacgcc tgacactcga ggtgcccggc ggctgggagg tggtggtgcg gcaccggaac
     3721 ctggatccca agcttcccca cagcgtcgag ctcatcggcg accgggaccc gctcccggtg
     3781 caggcggtcc cgccggtggt cccgggggcc gcgagcgccc ggctcctcga agggatcccg
     3841 ccgggaggtg tcgagacgat ccggttcact gcgccggacg gcgagtatcg gctcttctgt
     3901 gccgtgcccg gccatggcct cgcaggcatg tggctccggg tcagtgtgtc gacgcgcgtg
     3961 cgcacgccgg cactccgggc gacgccctag gcggcaccgc agcccgggtc accgcctggc
     4021 caccccttcg tcggccagga gcgccgtcag cgccgcctcg tccccacgtt cgaaggccgc
//
LOCUS       DASUAP010000158         6046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_876283, whole genome shotgun sequence.
ACCESSION   DASUAP010000158 DASUAP010000000
VERSION     DASUAP010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876283"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFU45_03900"
     CDS             complement(<1..905)
                     /locus_tag="VFU45_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU5358238.1"
                     /translation="MTAPRGIPAFAMDDELKRKLGFTILALVIYRVGAHIAAPGVNVQ
                     ALSDFINNSAAKGFFGLYDTLGGGLSRATVFALGIMPYISASIVLQLAGGMVPSVAKM
                     QQDEEGQKKITQWTRYITVMISLAQAYTFGLFIEQQIPGAVAQPGFSFLATTVLVLTT
                     GSIFVMWLGEQITERGIGNGMSLLITFSILERLWPGTINLVNYLKSGVVSFPAVVAWI
                     VALALAMAATTAMTIAARRIPINIPRKVMGRGRIQEGKKSFIPVMLLTASVMPIIFAQ
                     TIIIVPGTLASFTRAAWLQDLASTFT"
     gene            complement(909..1343)
                     /gene="rplO"
                     /locus_tag="VFU45_03905"
     CDS             complement(909..1343)
                     /gene="rplO"
                     /locus_tag="VFU45_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU5358239.1"
                     /translation="MTLSTLRPADGSAQSRKRVGRGIGSGLGKTSGKGHKGHKARTGG
                     ATNPGFEGGQMPMYRRLPKRGFTNPFRVAAQPVNLRDLGKVTAPEVNPETLHSAGLIA
                     KADAPVKILATGEIARALTVRGVRVSAAARAKIEAAGGRVEE"
     gene            complement(1373..1633)
                     /gene="rpmD"
                     /locus_tag="VFU45_03910"
     CDS             complement(1373..1633)
                     /gene="rpmD"
                     /locus_tag="VFU45_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU5358240.1"
                     /translation="MGRNPAVGRQGPAFHQIELPAKPVKGGLRVKQVRSTIGHADTYR
                     RTLAALGLHRHQQVVTVQDTPSIRGMLFKVRHLVDIVPTKEG"
     gene            complement(1635..2117)
                     /gene="rpsE"
                     /locus_tag="VFU45_03915"
                     /pseudo
     CDS             complement(1635..2117)
                     /gene="rpsE"
                     /locus_tag="VFU45_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(2308..2679)
                     /gene="rplR"
                     /locus_tag="VFU45_03920"
     CDS             complement(2308..2679)
                     /gene="rplR"
                     /locus_tag="VFU45_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU5358241.1"
                     /translation="MRAIHQPKTREERRYRRHLRVRKKLEGTTERPRLVVFRSLKHIS
                     AQLVNDETGKVLLGVTDNSDGVAPAAKGKVAKGKAVGKRLAELAKEQGITRAVFDRAG
                     YRYHGRVQAVADGAREGGLEL"
     gene            complement(2679..3218)
                     /gene="rplF"
                     /locus_tag="VFU45_03925"
     CDS             complement(2679..3218)
                     /gene="rplF"
                     /locus_tag="VFU45_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU5358242.1"
                     /translation="MSRIGKKPVVLPKGVTATVNGARVAIKGPKGELARTVHADMTVA
                     IAGDHLTVSRPSDEKRHKALHGLTRALLQNMVDGVHTGFAKTLELQGVGYKAEPKPYG
                     VNLVVGFSHQVPFHAPKGIKLTVDNNTVLKVEGPDKELVGQVAAEIRAVRPPEPYKGK
                     GIRYQGEQVRRKAGKTGAK"
     gene            complement(3230..3625)
                     /gene="rpsH"
                     /locus_tag="VFU45_03930"
     CDS             complement(3230..3625)
                     /gene="rpsH"
                     /locus_tag="VFU45_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU5358243.1"
                     /translation="MTMTDPVADMLTRIRNACMAGHRRVDMPASRLKGELARILRDNH
                     YIADFKLLEAEAQGVLRLYLKYHNELPVIRELQRVSKPGLRRYVKCREIPRVKNGLGM
                     AIVSTPKGLMTDREARTAKLGGELLAVVW"
     gene            complement(3639..3824)
                     /locus_tag="VFU45_03935"
     CDS             complement(3639..3824)
                     /locus_tag="VFU45_03935"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEU5358244.1"
                     /translation="MAKTCWIERAKRTPKFKVRTVRRCQRCGRSRAVLRKFGLCRICF
                     RDLALKGEIPGVRKASW"
     gene            complement(3832..4494)
                     /gene="rplE"
                     /locus_tag="VFU45_03940"
     CDS             complement(3832..4494)
                     /gene="rplE"
                     /locus_tag="VFU45_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU5358245.1"
                     /translation="MATTKEKEPRARGGRDKEKAAALQLDPAPQARVGKPRLQVHYET
                     VVRAALAKEFGLTNPLQIPRLVKVVLNVGMGEAQKAPKMLDAVVEELMIITGQQPVVT
                     RAKKAIANFGLRAGVPIGAAVTLRGARMYEFLDRFISVAVPRIRDFRGLSNKSFDGRG
                     NYSIGIKEQMIFPEIDFDKVEKVHGMDITVVTSTDRDDLAMALLRAFGWPFRGETPVR
                     VG"
     gene            complement(4495..4812)
                     /gene="rplX"
                     /locus_tag="VFU45_03945"
     CDS             complement(4495..4812)
                     /gene="rplX"
                     /locus_tag="VFU45_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU5358246.1"
                     /translation="MHLTKGDLVQVISGDDKGKQGRVLQVYPKTGRLIIEGVNLVKRH
                     LRATQTSEGGIITKPAPIHHSKAMLLDPKSGTPTRVRRRKAADGTAERVAAKSGQPIP
                     RNR"
     gene            complement(4880..5248)
                     /gene="rplN"
                     /locus_tag="VFU45_03950"
     CDS             complement(4880..5248)
                     /gene="rplN"
                     /locus_tag="VFU45_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU5358247.1"
                     /translation="MVQQESILRIADNSGARKALVIRVLGGSRRRYAGLGDRVVVTIK
                     DAIPTGQVKKGDVAKAVIVRTVKETRRKDGSYIRFDENAAVLINDQGEPRATRIFGPV
                     ARELRDRRYMKIVSLAPEVL"
     gene            complement(5299..5568)
                     /gene="rpsQ"
                     /locus_tag="VFU45_03955"
     CDS             complement(5299..5568)
                     /gene="rpsQ"
                     /locus_tag="VFU45_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU5358248.1"
                     /translation="MSETTTETRTRRKVRTGVVTSDKMTRTVTVLLTRRFAHATYGKQ
                     ITRTKKVHARDEHGAKVGDTVRIMETRPLSKTVRWRVTEIVERAK"
     gene            complement(5577..5777)
                     /gene="rpmC"
                     /locus_tag="VFU45_03960"
     CDS             complement(5577..5777)
                     /gene="rpmC"
                     /locus_tag="VFU45_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU5358249.1"
                     /translation="MKANEMTELSVAELQAKLAELREARVRLRFRSATEAIENPMQFR
                     TLRRDIARIETLLRARARAGQA"
     gene            complement(5781..>6046)
                     /gene="rplP"
                     /locus_tag="VFU45_03965"
     CDS             complement(5781..>6046)
                     /gene="rplP"
                     /locus_tag="VFU45_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU5358250.1"
                     /translation="RVAMTREMKRGGKVWIRIFPDKPITKKPAETRMGKGKGNPEGWV
                     AVVRPGRMVFELEGIPEDLARKALGLAASKLPLRTRFMKRLEV"
BASE COUNT          764 a         2191 c         2055 g         1036 t
ORIGIN      
        1 gtgaaggtgc tggcgaggtc ctgcagccag gcggcccggg tgaagctcgc cagcgtgccc
       61 ggcacgatga tgatggtctg cgcgaagatg atcggcatca cgctcgcggt gaggagcatg
      121 acggggatga acgacttctt cccctcctgg atccgccccc gccccatcac cttgcgcggg
      181 atgttgatcg ggatgcgccg cgccgcgatg gtcatcgccg tggtcgcggc catggccagc
      241 gcgagcgcca cgatccacgc cacgaccgcc gggaagctca ccaccccgct cttcaggtag
      301 ttgacgaggt tgatggtccc gggccagagc cgctcgagga tcgagaacgt gatgagcagg
      361 ctcatgccgt tcccgatccc gcgttcggtg atctgctcgc cgagccacat cacgaagatc
      421 gagccggtcg tgagcaccag gacggtcgtc gcgaggaagc tgaacccggg ctgcgccacc
      481 gcgccgggga tctgctgctc gatgaagagc ccgaaggtgt aggcctgcgc cagcgagatc
      541 atcaccgtga tgtagcgggt ccactgggtg atcttcttct ggccctcttc gtcctgctgc
      601 atcttggcga cgctcgggac catgccgccg gccagctgca ggacgatgct ggcggagatg
      661 tacggcatga tgcccagggc gaacaccgtg gcgcgcgaca ggccgccgcc cagggtatcg
      721 tacaggccga agaagccctt ggcggcggag ttgttgatga agtcgctgag cgcctggacg
      781 ttgacgcccg gcgcggcgat gtgggcaccg acgcgataga tcaccagggc gaggatggtg
      841 aacccgagct tgcgcttgag ctcatcgtcc atcgcgaagg ccggaatgcc gcggggagcg
      901 gtcacgacct actcctcgac gcggccgccg gcggcctcga tcttggcccg ggcggcggcc
      961 gagacgcgga cgccccggac ggtgagggcg cgggcgatct cgccggtggc gaggatcttc
     1021 accggggcat cggccttggc gatcagtccg gccgagtgga gcgtctccgg gttcacctcg
     1081 ggcgcggtga ccttgcccag gtcgcgcagg ttgacgggct gcgccgcgac ccggaacggg
     1141 ttggtgaagc cgcgcttcgg cagccggcgg tacatcggca tctggccgcc ctcgaagccc
     1201 ggattggtcg cgccgccggt gcgggccttg tggcccttgt gccccttgcc cgaggtcttg
     1261 cccaggccgg agccgatgcc gcgcccgacc cgcttgcggg actgggcgga cccgtccgcg
     1321 gggcgcagcg tgctgagcgt caccttggcc gcagccttgg tcttggtggc catcacccct
     1381 ccttcgtcgg cacgatgtcg accaggtgcc ggaccttgaa cagcatgccc cggatcgacg
     1441 gcgtgtcctg gaccgtcacc acctgctggt gccggtgcag gccgagcgcg gccagggtgc
     1501 gacggtatgt gtcggcgtgg ccgatggtgg aacggacctg cttgacgcgc aggccgccct
     1561 tgaccggctt ggccgggagt tcgatctggt ggaacgcggg gccctgacgg ccgaccgccg
     1621 gattacggcc catctcatgc ctcctggcgg ccgcggaacg ggacccgatc gacgtcgatg
     1681 ccgcgctcac gcgccacctg gcgcgcggtc acgagccggc cgagcccgtc catcgcggcc
     1741 cggacgacgt tgtgcgggtt gttcgagccg aggctcttgg taaggatgtc ggtgatcccg
     1801 gcgcacgcga ggagcgcacg caccgggccg ccggcgatga cgccggtacc gctcgccgcg
     1861 ggcttgagga gcacccggcc tgcgccgtgc gcaccgatga tctcgtgcgg gatggtggcg
     1921 ccgatgcggg gcacctcgat catggtgcgg cgggcggcgt cgaccgcctt ccgcaccgcc
     1981 tcggagacct cgttggcctt gccggtcgcg acgccgacct tccccttgcc gtcgcccacg
     2041 gcgacgagcg cgttgaacga gaaccgccgg ccgcccttga cgaccttggc cacccggttg
     2101 atggccacga cgttctcgac gaactcgctg tcacgctcgt cgcggccccg atcgccaccg
     2161 cggccgccgc ggccgcgctc gccgccccgc ccgccccgtt cgccgccacg cccccggccg
     2221 cccggtccgc gccggggcgc ctggccctgg ccctcggagg gcgcggtgcc ggtgggggcg
     2281 ccggtcgttt ccgcctgatc ggtcatctta cagctccagt ccgccctcgc gggcgccgtc
     2341 ggccacggcc tgcacgcggc cgtgatagcg atagccggcg cggtcgaaga ccgcgcgggt
     2401 gatcccctgt tccttggcca gctcggccag ccgctttccg accgccttgc ccttggccac
     2461 cttgcccttg gccgcgggcg ccacgccgtc ggagttgtcc gtcacgccga ggagcacctt
     2521 gccggtctcg tcgttgacga gctgggccga gatgtgcttg agcgagcgga acacgaccag
     2581 gcgcggccgc tcggtggtcc cttcgagctt cttccggacg cgcaggtgcc ggcggtagcg
     2641 ccgctcctcg cgcgtcttgg gctgatggat cgcacgcatc acttcgctcc cgtcttgccg
     2701 gccttgcggc ggacctgttc accctggtag cggatgccct tgcccttgta gggctcgggc
     2761 ggccggaccg cgcggatctc ggcggccacc tgccccacca gctccttgtc cgggccttcc
     2821 accttgagca ccgtgttgtt gtcgaccgtg agcttgatcc ccttcggcgc atggaacggc
     2881 acctggtggg agaacccgac gacgaggttg acgccgtacg gctttggctc ggccttgtag
     2941 ccgacaccct gcagctcgag ggtcttggcg aacccggtgt gcaccccgtc caccatgttc
     3001 tggaggaggg cgcgggtgag gccgtggagc gccttgtggc gcttctcgtc gctcgggcgc
     3061 gacaccgtga ggtggtcgcc ggcgatcgcc acggtcatgt ccgcgtgcac cgtgcgggcg
     3121 agctcgccct tgggcccctt gatggccacc cgggcgccgt tcaccgtggc ggtgacgccc
     3181 ttgggcagga cgacgggctt cttgccgata cgcgacatcg tccgccccct taccagacca
     3241 cggcgaggag ctcgccgccg agcttggccg tacgcgcttc gcggtcggtc atcaggccct
     3301 tgggcgtcga gacgatggcc atgccgaggc cgttcttgac gcgggggatc tcgcggcact
     3361 tcacgtagcg ccggagcccg ggcttgctga cgcgctgcag ctcgcggatc acgggcagct
     3421 cgttgtggta cttcaggtac aaccgcagca ccccctgcgc ctcggcctcg aggagcttga
     3481 agtccgcgat gtagtggttg tcgcgcagga tgcgcgccag ctcgcccttg agccgggacg
     3541 cgggcatgtc cacgcgccgg tgccccgcca tgcaggcgtt ccggatgcgg gtcagcatgt
     3601 ccgcgacggg atcggtcatc gtcatctgtc gtgtctcctc accaactcgc cttgcgcacg
     3661 cccgggatct cgcccttcag ggcgaggtcg cggaagcaga tccggcagag gccgaacttg
     3721 cgcaggaccg ctcgggaccg gccgcaccgc tggcagcggc gcacggtccg gaccttgaac
     3781 ttcggggtgc gcttggcgcg ctcgatccaa caggtcttcg ccatggtctc ctcagcccac
     3841 tcgcacgggg gtttcaccgc ggaacggcca gccgaacgcc cgaaggaggg ccatcgccag
     3901 gtcgtcccgg tccgtcgacg tcaccacggt gatgtccatc ccgtggacct tctccacctt
     3961 gtcgaagtcg atctcgggga agatcatctg ctccttgatg ccgatggagt agttgccccg
     4021 cccgtcgaag ctcttgttcg acaacccgcg gaagtcgcgg atccgcggca ccgccacgct
     4081 gatgaagcgg tccaggaact cgtacatccg ggcgccgcgg agcgtcacgg cggcgccgat
     4141 gggcaccccc gcccggaggc cgaagttggc gatcgccttc ttggcccggg tcaccaccgg
     4201 ctgctggccg gtgatgatca tcagctcctc gaccacggcg tcgagcatct tgggcgcctt
     4261 ctgcgcctcg cccatcccga cgttgagcac caccttgacc agccgcggga tctgcagcgg
     4321 gttggtcagg ccgaactcct tcgcgagcgc cgcgcggacc acggtctcgt agtggacctg
     4381 gagccggggc ttccccaccc gggcctgcgg cgccgggtcg agctgcagcg ccgccgcctt
     4441 ctccttgtcg cgcccgccgc gcgcgcgcgg ctccttctcc ttcgtcgtcg ccatttaccg
     4501 gttcctcggg atcggctgcc cggacttcgc ggccacgcgt tccgcggtgc cgtcggcggc
     4561 cttccgccgc cgcacccgcg tcggcgtccc gctcttgggg tccagcagca tcgccttcga
     4621 gtgatggatc ggggccggct tggtgatgat gccgccctcc gacgtctgcg tggcccggag
     4681 atgccgcttg accaggttca cgccctcgat gatcagccgg ccggtcttgg ggtacacctg
     4741 cagcacccgg ccctgcttgc ccttgtcatc gccgctgatg acctgcacca ggtcgccctt
     4801 ggtgaggtgc agcttgacgg cctgcggcac cggctggcgg cggtcgcggc gggacttgcg
     4861 atagacgagc ggcttcatgt cagagcacct ccggcgccag cgacacgatc ttcatgtagc
     4921 gccggtcgcg cagctcgcgc gccaccgggc cgaagatgcg ggtcgcccgg ggctcgccct
     4981 ggtcgttgat gaggacggcg gcgttctcgt cgaaccggat gtaggacccg tccttgcggc
     5041 gggtctcctt cacggtgcgc acgatgacgg ccttcgccac gtcgcccttc ttcacctgcc
     5101 cggtcgggat ggcgtccttg atcgtcacca cgacccggtc gcccagcccg gcgtaccgcc
     5161 gccggctgcc gcccagcacg cggatcacga gcgccttgcg cgcacccgag ttgtccgcga
     5221 tccggagaat cgattcctgt tggaccatga tatcccctgt ccgtcctcag ccgggcgccc
     5281 ccgtggggca ccccgtggtt atttcgcccg ctcgacgatc tcggtcaccc gccaccgcac
     5341 ggtcttcgac agcgggcggg tctccatgat ccgcacggtg tcgccgacct tggcgccgtg
     5401 ctcgtcgcgc gcgtgcacct tcttcgtgcg cgtgatctgc ttgccgtacg tcgcgtgcgc
     5461 gaagcgccgc gtcagcagca cggtcacggt ccgggtcatc ttgtcgctcg tgaccacgcc
     5521 ggtgcgcacc ttccgccggg tccgggtctc ggtcgtcgtc tcgctcatcg cgcgcctcac
     5581 gcctgtccgg cccgcgcacg ggcccgcagg agggtctcga tccgggcgat gtcgcgccgg
     5641 agcgtccgga actgcatcgg gttctcgatc gcctcggtgg ccgaccggaa gcgcagcctg
     5701 acccgcgcct cccggagctc cgccagcttg gcctggagct cggccaccga cagctcggtc
     5761 atctcattcg ccttcatcgg tcagacctcc agccgcttca tgaaccgggt ccgcagcggc
     5821 agcttcgacg ccgccaggcc gagcgccttc cgcgccaggt cctccgggat cccctccagc
     5881 tcgaacacca tccgcccggg ccgcaccacc gccacccagc cctccgggtt gcccttgccc
     5941 ttccccatcc gcgtctcggc cggcttcttg gtgatcggct tgtccgggaa gatccggatc
     6001 cacaccttgc cgccgcgctt catctcgcgg gtcatcgcca cgcggg
//
LOCUS       DASUAP010000159         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_576792, whole genome shotgun sequence.
ACCESSION   DASUAP010000159 DASUAP010000000
VERSION     DASUAP010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576792"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VFU45_03970"
     CDS             complement(<1..1345)
                     /locus_tag="VFU45_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5358251.1"
                     /translation="MNRLRFALRHAWREGRAAPRRLALLAGTVALGVGALTALNGFTD
                     NLRSSIKDSARSLMGADLSVSSNRPFTPAVDSVVTTAGAGGRTARIQSFGAMAFAPAG
                     KNTRLVDVEAVEGGWPFYGEVRTDPAGAWARLAEGGVALVDPVLLGGLGVHLGDTLAI
                     GNARVVVAGTLLKIPGDVGIRSAFAPRVYLSGHDASATGLLTFGSRVRYEAYVALPTG
                     ADVKAVAAKARPTLRTAGVGIRTASDDETDLDESLTTLARYLGLIALAALLLGGVGVA
                     SASHELISRRLETVAVLRCIGAPAGTVLTAYLVQAAAVGIAGGAVGAVGGTLLQLLIP
                     HLIRDFLPLDVRVVPSASAMLLGLGVGLWVTVAFALGPLLAVRQVPPIAALRRDLEAG
                     PAPRDRAQWIARLALGASVVLLAALQVRSARSAVGFSVALGLAVLALAVAARGLIA"
     gene            complement(1342..2013)
                     /locus_tag="VFU45_03975"
     CDS             complement(1342..2013)
                     /locus_tag="VFU45_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358252.1"
                     /translation="MLSSDHLTKTYPSGAGPLTVLKDITFTVDSGAFVAIVGPSGSGK
                     STLLGLLAGLDRPTGGRVILDGQDLGALDEDARALIRGRRVGFVFQAFQLIPTLTARE
                     NVQVPIELAGGADARVRADEILARVGLADRADHYPAQLSGGEQQRVALARAFANRPSI
                     LFADEPTGNLDAANGAAIIELLTGWNRDFGTTLVLVTHDPDLAARAGRVIRLSDGHLV
                     PDQAA"
     gene            2021..2773
                     /locus_tag="VFU45_03980"
     CDS             2021..2773
                     /locus_tag="VFU45_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEU5358253.1"
                     /translation="MSEVRGDNTPVPLKAKRALLAALTLAAACTSRAAPSSPPAAESV
                     AAATTADARPTIVFLGTSLTAGLGLDPGDAYPALIQQKLDSAHLAYRVVNAGVSGETS
                     AGALRRIDWVLQGPVGVLVIETGANDGLRGQDPDSTRAHIEAIISKARARRPVPAVAL
                     VQMRALPNYGPEYGRRFDRLYPAVADREHVTLFPFLLARVAGIDSLNQPDGIHPNVRG
                     ERIVADNVWHALLPLLDSISAQKNPLPPSGRN"
     gene            2837..3442
                     /locus_tag="VFU45_03985"
     CDS             2837..3442
                     /locus_tag="VFU45_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358254.1"
                     /translation="MQNWRRSLALVAALVWLVSLPAMAQGGMNGMDNDHPWWGHGVAF
                     WSQADPEGCWLCSPSIDINGGLFSSKDAASNSTNYAFVRLHTQIGLGIPYVALAGDLN
                     WIPTLGATPTFSMLAQFEPLRPRSRVYASAGLGLITGHNPTADRMEPWAQATLAYRSM
                     IHDITPFVQVGSALNSGQKMELLLGVAHPLAPYKMHWTLDK"
     gene            complement(3587..>4057)
                     /locus_tag="VFU45_03990"
     CDS             complement(3587..>4057)
                     /locus_tag="VFU45_03990"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEU5358255.1"
                     /translation="FATGGDTPDRDAAVGLLVRKFSAIALFGAGLLGVSGVITAWRHL
                     HRWSSLWTTPYGFALDAKLLVVLSIVTLGYYHWRKAGPGLTTPEAVKTFKVTAWRELK
                     LAGLVLLITSVLVSLPSPRFRPEGGPGRPGAERMQGPPRGGPEGAPRPGGGDRD"
BASE COUNT          621 a         1503 c         1396 g          537 t
ORIGIN      
        1 cggcgatgag cccccgtgcg gccactgcga gcgccagcac cgcgagcccg agcgccactg
       61 agaaccccac cgccgatcgg gcactccgca cctgcagggc cgccaggagc accacgctcg
      121 cgccgagcgc caggcgcgcg atccactgcg cgcggtcgcg cggggctggg ccggcctcga
      181 ggtcgcgccg gagcgccgcg atcggaggca cctgccgcac ggccaggagc ggccccaggg
      241 caaaggccac ggtcacccag agcccgacac cgagtcccag gagcatcgcg ctggcggacg
      301 ggacgacgcg cacatcgagg gggaggaagt cgcggatcag gtgcgggatc aggagctgca
      361 gcagcgtgcc ccccaccgcg ccgacggcac cgcccgcgat cccgaccgcc gcggcctgca
      421 ccaggtaggc ggtgagtacg gtgcccgccg gcgccccgat gcagcggagc accgccacgg
      481 tctcgagccg gcgggagatg agctcgtgcg acgcgctggc gacgcccacg ccacccagga
      541 ggagtgccgc gagcgcgatg aggccgaggt agcgcgccag cgtggtgagc gattcgtcga
      601 ggtcggtctc gtcatccgac gcggtgcgga tcccgacgcc cgcggtgcgg agcgtggggc
      661 gcgccttggc ggccacggcc ttgacgtcgg cgccggtggg cagggccacg tacgcctcgt
      721 accgcacccg cgagccgaag gtgaggagcc cggtcgccga cgcgtcgtgc ccgctgaggt
      781 acacccgcgg cgcaaaggcg ctccggatcc ccacgtcccc ggggatcttg agaagcgtcc
      841 ccgcgacgac cacccgcgcg ttcccgatgg caagggtgtc gcccagatgc acgccgagcc
      901 cgcccaggag gacggggtcc accagggcca cgcccccctc ggccagtcgc gcccacgcgc
      961 cggcggggtc ggtgcgcacc tcgccataga acggccaccc gccctcgacg gcctcgacgt
     1021 ccacgagccg ggtgttcttg ccggcggggg cgaatgccat cgcgccgaag ctctggatcc
     1081 gcgcggtgcg cccccccgca ccggccgtgg tcaccacgga atcgaccgcc ggggtgaagg
     1141 gccggttgct gctcacgctc aggtcggccc ccatcagcga acgcgccgag tccttgatgg
     1201 agctgcggag gttgtcggtg aatccgttga gcgccgtgag cgcccccacg cccagcgcca
     1261 cggtgccggc caggagggca aggcggcggg gcgcggcccg gccctcgcgc caggcgtgcc
     1321 ggagcgcaaa gcggagccgg ttcacgcggc ctggtccggg accaggtgcc cgtcggacag
     1381 ccggatgacc cgtccggcgc gcgcggcgag gtcgggatcg tgggtcacaa ggacgagcgt
     1441 cgtgccgaag tcgcggttcc atcccgtcag gagctcgatg atcgcggcac cgttcgccgc
     1501 atcgaggttc ccggtcggct cgtccgcaaa gaggatgctc ggccggttgg caaaggcccg
     1561 cgccagcgcc acccgctgct gctcaccacc ggagagctgc gcggggtagt ggtcggcccg
     1621 gtcggccagg cccacccgcg cgaggatctc gtcggcgcgg acacgggcgt cggcgccccc
     1681 cgccagctcg atcgggacct gcacgttctc ccgcgcggtg agcgtcggga tgagctggaa
     1741 ggcctggaaa acaaacccca cccgccggcc gcgaatgagc gcccgcgcat cctcgtcgag
     1801 cgcacccagg tcctggccgt cgaggatcac ccggcccccg gtggggcggt cgagcccggc
     1861 caggaggccc agcagcgtgc tcttcccgct ccccgaaggg ccgacgatgg ccacgaaggc
     1921 gccggagtcc acggtgaagg tgatatcctt cagcaccgtg agcggccccg cgccggatgg
     1981 ataggtcttg gtcaggtgat cagaggagag catccgcccg atgtccgagg ttcgaggtga
     2041 caacacgccg gtcccgctca aagctaagcg cgccctcctc gcggcgttga cgctcgcggc
     2101 ggcctgcacc tcccgtgcgg ccccctcgag cccacccgcg gccgagtccg tcgcggccgc
     2161 gaccaccgcc gacgcccggc cgacgatcgt cttcctcggc accagcctca ctgccgggct
     2221 cggcctcgat cccggcgacg cctatcccgc cctgatccag cagaagctcg actcggcgca
     2281 cctcgcctat cgcgtcgtca acgccggggt gagcggtgag acctccgccg gcgccctccg
     2341 ccggatcgac tgggtgctgc aagggccggt gggggtgctc gtgatcgaga ccggcgccaa
     2401 cgacgggctc cgaggccagg atcccgactc cacccgcgcc cacatcgagg ccatcatttc
     2461 gaaggcgcgc gcgcgccggc cggtgccggc cgtggcgctg gtccagatgc gggcgctccc
     2521 caactacggc ccggagtacg gtcgtcgctt cgatcggcta tacccggcgg tcgccgatcg
     2581 cgaacacgtc accctcttcc cgttccttct ggcccgcgtg gccggcatcg actcgctcaa
     2641 ccagcccgac ggtatccacc ccaacgtgcg cggtgagcgc atcgtggccg acaacgtctg
     2701 gcacgcgctc ctccccctcc tcgactccat ctccgcgcaa aagaatccgc tcccgccctc
     2761 cgggcgtaac tgagacatcc cggccggtgc ggatcggggc cttcagtgta acccattgta
     2821 atttcaggag atagcaatgc agaattggcg tcgcagcctt gccctcgtag ctgccttggt
     2881 gtggctggtg agcctgccgg cgatggcgca gggagggatg aacgggatgg acaacgacca
     2941 tccgtggtgg ggccacggcg tggcgttctg gtcgcaggcg gacccggaag ggtgctggct
     3001 ctgttccccg tcgatcgaca tcaacggcgg cctcttctcg tcgaaggacg ccgccagcaa
     3061 ctcgaccaat tacgccttcg tccggctcca cacccagatc gggctcggca tcccctacgt
     3121 ggcgctggcc ggcgacctca actggatccc gaccctcggc gccacgccca ccttctcgat
     3181 gctggcgcag ttcgagccgc tccgtccccg gtcacgggtg tacgcgagcg cggggctcgg
     3241 tctcatcacc gggcacaacc cgaccgccga ccggatggag ccgtgggccc aggccacgct
     3301 ggcctaccgg tcgatgatcc acgacatcac gccgttcgtg caggtcggga gcgcgctcaa
     3361 cagcggccag aagatggagc tcctcctcgg cgtggcccac ccgctggcgc cgtacaagat
     3421 gcattggacg ctggataagt agctatcagc tatcggctat cagctatctg cggctccata
     3481 agccaacggg cccccgtgga catccacggg ggctcgcagc aattgcgcca tgggcgctgg
     3541 attcacacct tcgcgctgat agctgatagc tgatagctga tagctgtcag tccctatccc
     3601 caccacccgg cctcggcgcc ccctccggcc ctccacgcgg cggaccctgc atccgctcgg
     3661 cgccggggcg gccggggcca ccctcgggcc ggaaccgcgg cgaagggagg ctcaccagca
     3721 ccgacgtgat caggagcacc agccccgcca gcttgagctc gcgccacgcg gtgaccttga
     3781 acgtcttcac cgcctccggg gtggtgaggc cggggcccgc cttccgccag tggtagtagc
     3841 ccaatgtcac gatggatagc accaccagga gcttggcatc gagcgcaaag ccatatggcg
     3901 tggtccagag ggagctccag cggtggaggt ggcgccaggc ggtgatgacc cctgacacgc
     3961 cgaggagccc cgcgccgaag agtgcgatgg cggagaactt ccgcaccagg aggccgaccg
     4021 cggcatcgcg gtcgggggtg tctccccccg tggcgaa
//
LOCUS       DASUAP010000160         4990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1026176, whole genome shotgun sequence.
ACCESSION   DASUAP010000160 DASUAP010000000
VERSION     DASUAP010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026176"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2356)
                     /locus_tag="VFU45_03995"
     CDS             complement(<1..2356)
                     /locus_tag="VFU45_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5358256.1"
                     /translation="MSIARLVERHRSAVYLSLALLTLGGLLALFTLPAGIYPEVAYPR
                     VVVVAKQGTFEVQDMTVAVTRPIEEAMSGIIALQRIRSRTVRGATEISLDFAPNADMQ
                     YSLTQVQGRLAQLEPSLPSGLDITTERLTPSVFPIIQYELRGADPVQLRDIAQYQIRP
                     RLARLPQVGEVEIQGGLVREVSVVLDPERLVSSHIGVEEVAQAIDRSDVVIAAGRVDR
                     DYRQSSIIVSGLASTPAAVAAIVVKQAGPRAIRVGDLGTVRYGAEDQFQLITGDGEPA
                     ALINVARQPNGNAVRIDDEVRAALDSVRPILPAGAELKEVYNQAALVRESMTSVRDAM
                     LVGGALAVLILFLFLGRWNTTFAAALSLPLAVIGTFAVLTLAGQSLNLMSLGGLAVAI
                     GLIIDDAVVVVENIERRLALHPEEPPDEVIRRGTDEIFGPVAGSTLTTVVVFAPLGLL
                     QGVVGAFFQSFSVALAASVLLSLAIAMTLIPALVSQMVRRARARGEAPAPHRPVIDLH
                     RIETWYAALADRLIGRRRLVLGIAAGLLIGGFGMMKLVDTGFLPEMDEGGFVLDYWTA
                     TGSSLAETDREVHVIEGILGRDPDIQAFSRRTGAELGFAATAPNRGDFTVLLKPLSRR
                     HASVYEVMDRVRAQVAVQTPTVRVEFAQLMQDALADLAGAPSPIEIKLFSPDREASEE
                     AARRVAAAVDSTPGMVDLFNGVVGPSPVVLVQLDPARVARLGLTPADVLDQARGALFG
                     ASAGSVREPDRLVPVRVRLPDSVRYRADIAATLPVVGPDRWAPLG"
     gene            complement(2353..3417)
                     /locus_tag="VFU45_04000"
     CDS             complement(2353..3417)
                     /locus_tag="VFU45_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5358257.1"
                     /translation="MTRRILCSLVILATVAACTRRSGGDDDDASAGAPPGATAVAVAL
                     APVRRDTVSDVLELVGRLEPAPGAGAILAAPTAAVVHQVRVQVGASVQAGQALIELDA
                     PELSAQARSDSAAAAVAEREAARQRSLLAQGIASAKQADEAVNAATAARAAADASARL
                     LARATVRSPIDGLVNDVRVQPGERVDAGAPLATVLDPDTLDLVVPVPAARLARIHRGL
                     SAWITAEGDTVRHRAEVIGVAPGVDSVTNAGRIVLRMPNPGRRVHPGAGATASLVLSL
                     TRDALVVPASALVLIGDQQAVFVVGRDSIAHSRVVTVAVHAHDRAAVVGALKAGEQVV
                     ALGGAGLADGMRVAPAAARP"
     gene            complement(3423..4604)
                     /locus_tag="VFU45_04005"
     CDS             complement(3423..4604)
                     /locus_tag="VFU45_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5358258.1"
                     /translation="MLTLVLALALNGAAAQDSVTLAEALARARAHRPLVAEAAAGVAE
                     ARGAARTGTTVPNPVFSYTWTGDTPREHATVTQPLDWLPRHGNDRAAGRAGIARAEAD
                     STATMATLARDVRVAFYDAVAGRRALALTEDEAALADSLARAADARLAAGDISELERD
                     QVAQEAARARYAVSLAHEDARVAQGALTRALGASTPVEPAGALDDGLDAAPPAATAID
                     ALPSLRVVLADSAASAARARAARISQFPLPDLQGGVEWNDPTNPNQGATELLGLAIPL
                     PLWNHGGGAAAETLAQARRDAAAAGEARLEAARALAERAARLEGAALRARFDRDSLYP
                     AARQLRERAIAAYRAGATGVLPVFDAMRGERDAALTLVRDLVAYQDALADWNALLGRT
                     D"
     gene            complement(4667..>4990)
                     /locus_tag="VFU45_04010"
     CDS             complement(4667..>4990)
                     /locus_tag="VFU45_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5358259.1"
                     /translation="GDAMLLREAVMELIENACRHSSGDGPVRIGVTARDRAAVISVEN
                     PGPALPADWEARAPGPHAGSGSEPRGLGLALLRWIAAVHGGRLSATHSGGRNTISLEI
                     PLQPR"
BASE COUNT          686 a         1794 c         1894 g          616 t
ORIGIN      
        1 cgcccagcgg ggcccagcga tcgggaccca ccaccggcag ggtggcagcg atgtcggccc
       61 ggtaacgcac ggagtcgggg aggcggaccc gcaccgggac gaggcggtcg ggttcgcgta
      121 ccgacccggc ggaagctccg aagagcgcgc ctcgggcctg gtcgagcacg tcggcggggg
      181 tgaggcccaa ccgggcgacc cgcgccgggt cgagctggac cagcaccacc gggctcggac
      241 ccaccacacc attgaaaaga tccaccatcc ctggtgtgct gtcgacggcg gcggccaccc
      301 gccgtgccgc ctcctcggat gcctcgcggt cggggctgaa gagcttgatc tcgatggggg
      361 agggggcccc tgcgaggtcg gcgagcgcgt cctgcatcag ctgcgcgaat tccacccgga
      421 cggtcggcgt ctggacggcc acctgcgcgc ggacccggtc catcacctcg tagaccgacg
      481 cgtggcggcg gctgagcggc ttgagcagta cggtgaagtc gccccgattg ggcgcggtcg
      541 ccgcgaagcc aagctccgcg ccggtccggc gggaaaaggc ctggatgtca gggtcgcgcc
      601 cgaggatccc ctcgatgacg tggacctccc ggtcggtctc ggccagcgag ctgccggtcg
      661 cggtccagta gtcgaggacg aacccgccct cgtccatttc cgggaggaag ccggtgtcga
      721 ccagcttcat catcccgaag ccaccgatga ggagccccgc ggcgatgccc agcaccaggc
      781 ggcgccgccc gatcagtcgg tctgccagcg cggcgtacca ggtctcgatg cggtggaggt
      841 cgatgacggg gcggtggggc gccggcgcct ccccccgtgc gcgggcccgc cggaccatct
      901 ggctcacaag cgcggggatg agtgtcatcg cgatggcgag cgacaggagc accgacgccg
      961 ccagcgccac ggagaacgac tggaagaacg cgccgaccac tccctgcagc aggccgaggg
     1021 gcgcaaagac cacgacggtc gtgagcgtgg accccgccac cggcccgaag atctcgtcgg
     1081 tgcctcggcg gatcacctcg tccggaggtt cctccggatg gagcgccagg cgacgctcga
     1141 tgttctcgac cacgaccacc gcgtcgtcga tgatgaggcc gatggcgacg gcgagcccgc
     1201 cgagcgacat caggttgagc gactggcccg cgagcgtaag gacggcgaag gtcccgatca
     1261 cagcaagggg gaggctgagc gccgctgcga acgtggtgtt ccagcgcccg aggaagagga
     1321 agaggatcag gaccgccagc gcgccgccga cgagcatcgc atcgcgcacg ctcgtcatcg
     1381 attcccgcac cagcgctgcc tggttgtaca cctccttgag ctcggccccc gccggtagga
     1441 tcggccgcac cgaatcgagc gcggcccgga cctcatcgtc gatccggacg gcgttgccat
     1501 tcggctggcg ggcgacgttg atgagcgccg ccggctcccc gtcgccggtg atgagctgga
     1561 actggtcttc ggcgccgtag cggaccgtgc cgaggtcgcc gacccggatg gcgcgggggc
     1621 ctgcctgctt caccacgatc gcggcgaccg ccgcaggggt cgaggcgagc cccgagacga
     1681 tgatgctcga ctgccggtag tcgcgatcga cccgcccggc ggcgatgacc acgtccgagc
     1741 ggtcgatggc ctgcgccacc tcctcgacgc cgatgtggga gctcacgagg cgctcggggt
     1801 cgagcaccac cgagacctcg cggacgaggc ccccctggat ctcgacctcg cccacctggg
     1861 gcagccgggc gagccgcggc cggatctgat actgcgcaat gtcgcggagc tggaccgggt
     1921 cggccccgcg aagttcatac tggatgatcg ggaacaccga tggcgtcagg cgctcggtgg
     1981 tgatgtcgag gccggacggg agcgatggct cgagctgcgc caggcgcccc tgcacctggg
     2041 taagggaata ctgcatgtcg gcgttgggcg cgaagtcgag cgagatctcg gtggccccgc
     2101 ggaccgtgcg cgaccggatc cgctgcaggg cgatgatgcc gctcatcgcc tcctcgatcg
     2161 ggcgggtgac ggcgaccgtc atgtcctgca cttcgaaggt gccctgcttg gccacgacca
     2221 ccacacgcgg gtacgccacc tccgggtaga tccccgccgg gagcgtgaag agcgccagca
     2281 gtccgcccag cgtgaggagc gcgagcgaga ggtagaccgc cgagcggtgc cgctcgacca
     2341 gccgggcgat gctcacgggc gcgctgccgc cggtgccacc cgcatcccat cggcgaggcc
     2401 ggcgcctcca agggccacga cctgttcccc ggccttgagc gcgccgacca cggccgcgcg
     2461 gtcgtgggcg tggacggcga cggtcacgac gcggctgtgc gcgatcgaat cgcgccccac
     2521 cacgaacacc gcctgctggt ccccgatcag caccagggcg cttgcgggca ccacgagtgc
     2581 gtcgcgggtc aggctgagga cgaggctggc cgtggcgccg gccccgggat gcacgcggcg
     2641 gccgggattc ggcatccgga gcacgatgcg cccggcgttg gtgaccgaat ccacgcccgg
     2701 cgccacgccg atcacctccg cccgatgccg gacggtgtcg ccctcggcgg tgatccacgc
     2761 cgacaggccg cggtggatgc gcgcgagcct ggcggcgggc accggcacca cgaggtcgag
     2821 ggtatcgggg tcgagcaccg tcgcgagcgg tgcgcccgcg tcgacccgct cgcccggctg
     2881 gacccggacg tcgttcacca ggccgtcgat cgggctccgc accgtggcgc gggccagcag
     2941 cctggcgctg gcatctgcgg cggcgcgggc cgcggtcgcc gcgttcaccg cctcgtcggc
     3001 ctgcttggcc gaggcgatcc cctgcgccag gagcgaccgc tggcgggccg cctcgcgctc
     3061 ggcgacggcg gcggcggcgg aatcggaccg ggcctgtgcg ctgagctcgg gggcgtcgag
     3121 ttcgatcagc gcctggccgg cctgcacgct cgccccgacc tggacccgga cctggtggac
     3181 gaccgcggcg gtgggcgccg ccaggatggc gccggcgccg ggcgccggtt cgagtcggcc
     3241 gaccagctcg agcacgtcag acaccgtgtc gcgccgcacc ggcgcgagcg ccacggccac
     3301 cgccgtggcc ccgggcggcg cgccggccga cgcatcgtcg tcgtcaccgc cggagcgccg
     3361 ggtacaggcg gccacggtgg ccaggatcac gagcgaacag aggattcggc gcgtcatcgg
     3421 catcagtccg tccggcccag gagggcgttc cagtcggcga gtgcgtcctg gtaggccacg
     3481 aggtcgcgca cgagcgtgag cgcggcgtcg cgctcccccc gcatggcgtc gaacacgggg
     3541 agtacgccgg tggctccggc gcggtaggcc gcgatcgcgc gctcccggag ctggcgggcg
     3601 gcgggataga gcgagtcgcg atcgaatcgc gcgcgcaacg ccgctccctc gaggcgcgcg
     3661 gcacgctcgg cgagcgcgcg tgcggcctcg agccgggcct cgccggccgc ggccgcgtcc
     3721 cgccgcgcct gggccagcgt ctcagccgcg gcgccgccac cgtggttcca cagcgggagc
     3781 gggatggcca agcccaggag ctcggtggcg ccctgattcg ggttcgtggg gtcattccac
     3841 tcgacgccgc cctgcaggtc cgggagcggg aactgggaga tgcgggcagc gcgggcgcgg
     3901 gcggccgacg ccgccgaatc ggcgagcacg acgcggagcg acggaagggc gtcgatcgcc
     3961 gtcgcggccg gtggcgccgc gtcgagcccg tcgtcgagcg cgccggccgg ttccacggga
     4021 gtggatgcgc cgagcgcccg cgtcagggcc ccctgtgcca cgcgggcgtc ttcgtgcgcg
     4081 agcgacaccg cgtagcgggc gcgcgccgcc tcctgcgcca cctggtcgcg ctcgagctcg
     4141 gagatgtctc cggcggcgag gcgcgcgtcc gcggcccgtg cgagcgaatc ggccagggct
     4201 gcctcgtcct cggtcagcgc cagcgcgcgc cggccggcca cggcgtcgta gaacgcgaca
     4261 cgcacgtcac gcgcgagcgt cgccatcgtg gcggtcgagt cggcctccgc gcgcgcgatc
     4321 ccggcccgcc cggcggcgcg gtcgttcccg tgccggggca gccagtcgag cggctgggtg
     4381 actgtggcgt gctcgcgcgg cgtgtcgccg gtccaggtgt aggaaaagac cgggttcggc
     4441 acggtggtcc cggtccgtgc ggcgccccgc gcctccgcca cgccggccgc cgcctccgcc
     4501 accagcggcc gatgcgcccg cgcgcgcgcc aaggcctcgg ccagcgtgac cgagtcctgg
     4561 gcggcggcgc cgttgagcgc cagggcgagg accagcgtga gcaatcggtg tcccgggtgc
     4621 ggggggcctg atactacccg ggccagatga cggcatcgtg acgggatcag cggggttgga
     4681 gcgggatctc cagggagatg gtattccggc cgccggagtg ggtggcggag aggcggccgc
     4741 catgcaccgc cgcgatccag cggaggagtg ccaggcccag cccccgtggc tcagatcctg
     4801 atccggcgtg aggtccggga gcgcgcgcct cccagtcggc cggcagggcc gggccgggat
     4861 tctccaccga gatcaccgcg gcgcggtccc gggcggtgac gccgatgcgg accggcccat
     4921 cgcccgagct gtgacggcag gcgttttcga tgagctccat caccgcctcc cgaaggagca
     4981 ttgcgtcccc
//
LOCUS       DASUAP010000161         3903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1121917, whole genome shotgun sequence.
ACCESSION   DASUAP010000161 DASUAP010000000
VERSION     DASUAP010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121917"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..925)
                     /locus_tag="VFU45_04015"
     CDS             complement(35..925)
                     /locus_tag="VFU45_04015"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEU5358260.1"
                     /translation="MSHDAHRAIEAVWRIESARLIAGLTRFARDVGLAEELAQEALLA
                     ALEQWPRDGVPDNPGAWLMSVARRRGIDLWRREERIAPKHAEIGRDLDAARERELDRL
                     DESLDDPLGDDLLRLFFLTCHPVLSREARVALTLRLVAGLTTPEIARAFFVPEPTLAQ
                     RIVRAKRTLAEARVPFELPPPGELGARLASVLEVIYLVFNEGYSATAGEEWIRADLCE
                     EALRVGRILAELAPMEPEVHGLVALMEIQASRSRARVGPGKEAIRLLDQDRARWDWVL
                     VNRGLAALERAERLGGALGP"
     gene            complement(922..1281)
                     /locus_tag="VFU45_04020"
     CDS             complement(922..1281)
                     /locus_tag="VFU45_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5358261.1"
                     /translation="MRYLTFVRGREDQGAPSPALAAAMQKFIAESLADGTLVQTGGLS
                     RYPDATLLRIKQGKLSVTDGPFAEAKEVIGGYAIIEASSKEEAIRIGTAFMKLHSEHW
                     PEWEGESEIRQIDFLVP"
     gene            complement(1284..1673)
                     /locus_tag="VFU45_04025"
     CDS             complement(1284..1673)
                     /locus_tag="VFU45_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5358262.1"
                     /translation="MRFLMLVIPKAYETADANFVPPADLVAKMTKFNESLRKAGVLLS
                     LDGLHPPARAARVRFGGGKATVTDGPFAEAKEMVGGFWMIQVKSREEALEWARRAPML
                     DGDIIEVRQVQELEEFPEDVRRAAGGK"
     gene            complement(1685..2077)
                     /locus_tag="VFU45_04030"
     CDS             complement(1685..2077)
                     /locus_tag="VFU45_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5358263.1"
                     /translation="MSTTTRRANAILWIVQSLLAALFLYAGGFKLATPLAALAKMSPF
                     PAPFLRFVGICEVAGALGLILPGLLGIKRGLTPLAATGLVGIMVGAVTATIATLGVLP
                     ATFPLVVGLLAAAVARRRWSRTVRLPNR"
     gene            2388..3503
                     /locus_tag="VFU45_04035"
     CDS             2388..3503
                     /locus_tag="VFU45_04035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU5358264.1"
                     /translation="MSRVRVLVGTRKGAFILTSDGKRKNWEVQGPHFAGWEIYHVKGS
                     PVDPDRIYASQSSGWFGQQVPRSDDGGKTWTPVENKFAYDGTPGTHQWYDGTPHPWEF
                     KRVWHFEPSRTEADTVYAGVEDAAIFKSTDGGKSWNELPGLRGHGTGPHWQPGAGGMC
                     LHTILLDGKDPKRIFVAISAAGAFRSDDGGKSWKPINKGLHSKFLPDPGAEVGHCVHR
                     IAQHKGKPNTLFMQKHWDVMRSDDAGDTWHEVSGNLPTDFGFVIDVHAHEPETVFVVP
                     IKSDSEHFPLDGKLAVYRSKTGGNEWQAMTKGLPQKDCYVNVLREAMSVDSLDPCGIY
                     FGTTGGQVYGSADGGESWAPLVRDLPAVLSVEVQSLP"
     gene            3500..3796
                     /locus_tag="VFU45_04040"
     CDS             3500..3796
                     /locus_tag="VFU45_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5358265.1"
                     /translation="MIRVELPHHLRTLAGTGPEVTLDAGPAPTQRSVLDALEARYPML
                     RGTIRDHTTHKRRAFLRFFACGKDLSHESPDTPLPAAVASGAEPYLVIGAIAGG"
BASE COUNT          605 a         1369 c         1351 g          578 t
ORIGIN      
        1 gggcgtggca cgcggcgatc gccgcctgca gcgcttatgg cccgagcgcg ccgccgagcc
       61 gctcggcgcg ctcgagcgcc gccagtccgc ggttcaccag cacccagtcc cagcgggcgc
      121 ggtcctggtc gaggaggcgg atggcctcct tcccaggccc gacgcgggcc cgcgaccggg
      181 aggcctggat ctccatcagc gccacgagcc cgtggacctc gggctccatc ggcgccagct
      241 ccgccaagat acgccccacc cggagcgcct cctcgcagag gtcggcccgg atccactcct
      301 cgccggcggt ggcggagtac ccctcgttga acacgaggta gatcacctcc agcaccgacg
      361 cgaggcgggc gccgagctcg cccgggggcg ggagctcgaa cgggacgcgc gcctcggcca
      421 gcgtccgctt ggcccggacg atccgctgcg ccagggtcgg ctcggggacg aagaaggcgc
      481 gcgcgatctc gggcgtcgtg agtccggcca cgagcctgag cgtcagcgcc acccgcgcct
      541 cccgggagag taccgggtgg caggtgagga agaagaggcg gaggaggtcg tcgcccagcg
      601 ggtcgtcgag ggattcgtcg agccggtcga gctcgcgttc gcgcgcggcg tcgaggtcgc
      661 ggccgatctc cgcgtgcttc ggcgcgatcc gctcctcccg ccgccacagg tcgatgcccc
      721 gccgcctggc caccgacatc agccaggcgc ccgggttgtc cgggacaccg tcccggggcc
      781 actgctcgag cgcggcgaga agcgcctcct gagccagctc ctcggcgagc cccacgtccc
      841 gcgcaaagcg agtcagcccg gcgatcaacc gggccgactc gatcctccag accgcctcga
      901 tggcgcggtg tgcgtcgtga ctcacggcac caggaagtcg atctgccgga tctcgctctc
      961 tccctcccat tccggccagt gttcggagtg gagcttcatg aaggcggtgc cgatcctgat
     1021 cgcctcctcc ttcgagctgg cctcgatgat ggcgtagccg ccgatcactt ccttcgcctc
     1081 ggcgaaggga ccgtcggtga ccgagagctt cccctgcttg atccggagga gcgtggcgtc
     1141 ggggtagcgg gagagcccac ccgtctggac cagcgtgccg tcggcgagcg actcggcgat
     1201 gaacttctgc atcgccgctg cgagcgccgg cgatggggcg ccctggtcct cgcgcccccg
     1261 gacaaaggtc aggtaccgca tcgtcacttc cctcccgcgg cgcgccgcac gtcctcgggg
     1321 aactcctcga gctcctggac ctggcgcacc tcgatgatgt cgccgtcgag catcggcgcg
     1381 cgccgggccc actcgagcgc ctcctcccgc gacttgacct ggatcatcca gaatccgccg
     1441 accatctcct ttgcctcggc gaaggggccg tcggtgaccg tcgccttccc gccgccgaag
     1501 cggacccgcg ccgccctggc aggagggtga aggccgtcga ggctcaggag gacgcccgcc
     1561 ttgcgcagcg actcgttgaa cttcgtcatc ttggccacca ggtcggcggg cgggacgaag
     1621 ttcgcgtccg ccgtctcgta cgccttgggg attaccagca tcaggaatcg catcgtgtat
     1681 ctccttatcg gttcgggagc cggacggtcc ggctccaccg ccgccgtgcc accgccgccg
     1741 cgaggagtcc gacgaccagc gggaaggtcg cggggaggac gcccagcgtg gcgatcgtgg
     1801 cggtgactgc cccgaccatg atcccgacca ggcccgtggc ggcgagcggc gtcaaccctc
     1861 tcttgatacc caggagcccc gggagtatca agccgagtgc gcccgcgacc tcgcagatcc
     1921 cgacgaagcg gaggaacggg gcgggaaagg ggctcatctt tgccaaggcg gcgagcgggg
     1981 tcgcgagctt gaacccgccg gcatagagga agagcgcggc gaggaggctc tgcacgatcc
     2041 agaggatcgc gttcgcgcgg cgggtggtgg tggacatctg ccatctcctg gtccgggtcg
     2101 gagccggccc gttccgggct ccattgatac gtcgaacggg gcagggtgat ttcgacatgg
     2161 catataagcc attgtgttac aaagcgttaa ggcacaacgt ggatcagtcc ggccgtgcca
     2221 gactcaccgc ccactaccac gccggcttct ccgcccgctc tcccgcccgc tttttcgccc
     2281 gctcgctcgc ccgcccaccg ttgacacgcc ccccgtgcgg cccgaccttt tcgattcgtc
     2341 ttgcacgttg tatcagagaa cgccaagcca cgcctttggg ggatccgatg agtcgagttc
     2401 gcgtgctggt cggcaccagg aagggtgcgt tcatcctcac ctccgacggg aagcggaaga
     2461 actgggaggt gcaggggccg cacttcgccg ggtgggagat ctatcacgtg aagggctcgc
     2521 cggtggaccc cgaccggatc tacgcctcgc agtcgagcgg atggttcggt cagcaggtgc
     2581 cccgctcgga cgacggtggc aagacctgga ccccggtgga gaacaagttc gcctacgacg
     2641 ggacgccggg cacgcaccag tggtacgacg gcacgccgca tccgtgggag ttcaagcggg
     2701 tgtggcactt cgagccctcg cgcaccgagg cggacacggt gtacgccggg gtcgaggacg
     2761 ccgcgatctt caagtccacc gacggcggca agagctggaa cgagctcccc ggtctccggg
     2821 gccacgggac cggtccccac tggcagccgg gtgccggcgg gatgtgcctg cacaccatcc
     2881 tgctcgacgg caaggacccg aagcggatct tcgtcgcgat ctccgcggcg ggagcgttcc
     2941 gcagcgatga cggcggcaag agctggaagc cgatcaacaa agggctccat tccaagttcc
     3001 ttcccgatcc cggggccgaa gtggggcatt gcgtccatcg catcgcccag cataagggga
     3061 agccgaacac cctcttcatg cagaagcact gggacgtgat gcggagcgac gatgccggcg
     3121 acacgtggca tgaggtcagc gggaacctcc ccaccgactt cggcttcgtg atcgacgtcc
     3181 acgcccacga gccggagacg gtgttcgtgg tgccgatcaa gagcgactcg gagcacttcc
     3241 cgctggacgg gaagctcgcc gtgtaccgga gcaagaccgg cgggaacgag tggcaggcga
     3301 tgaccaaggg cctgccgcag aaggactgct acgtcaacgt cctccgcgag gcgatgtcgg
     3361 tggattccct tgatccctgc ggcatctact tcggcacgac cggcgggcag gtgtacgggt
     3421 cggccgacgg cggagagagc tgggcgccgc tggtgcgcga ccttccggcc gtgctctcgg
     3481 tcgaggtgca gtcgctcccg tgatccgggt ggagctgccg catcacctcc ggaccctggc
     3541 cggaacgggc ccggaggtga ccctcgacgc ggggccggcg ccgacgcagc ggagcgtcct
     3601 cgacgcgctc gaggcgcgct accccatgct ccgcggcacc atccgcgatc acacgaccca
     3661 caagcgccgg gcgttcctcc ggttcttcgc ctgcgggaag gacctctcgc atgagtcgcc
     3721 ggacacgccg ctccccgctg cggtggcctc cggcgccgag ccgtacctcg tcatcggcgc
     3781 gatcgcgggg ggatgagacg taagacgtga gaggtgagac gtgagccgta ggggcgcggc
     3841 atgccgcgcc cgtgcgctgt catcggccga tgcgcgcgac gtgatccgcc agccgcccca
     3901 ggg
//
LOCUS       DASUAP010000162         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_45454, whole genome shotgun sequence.
ACCESSION   DASUAP010000162 DASUAP010000000
VERSION     DASUAP010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45454"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /gene="pdxH"
                     /locus_tag="VFU45_04045"
     CDS             complement(<1..619)
                     /gene="pdxH"
                     /locus_tag="VFU45_04045"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEU5358266.1"
                     /translation="MADLADLRREYKRARLDLADLGPDPLAAFERWFAEARAAEALDP
                     SAMALATADEHGAPSVRMVLLKGFDKRGFVFFTDRRSGKGSELAVNPHAALLFWWPEL
                     ERQVRVTGAVSRTTDAESEAYFRSRPLGSRLGAWASHQSAVVESRDAIETAAAQAAHR
                     YGEDPPLPPHWGGFRVTPEAFEFWQGRESRLHDRLRYRREAGKWVV"
     gene            complement(619..1026)
                     /gene="apaG"
                     /locus_tag="VFU45_04050"
     CDS             complement(619..1026)
                     /gene="apaG"
                     /locus_tag="VFU45_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEU5358267.1"
                     /translation="MTPLPFYYRETDGIRITVRPAYLPEQSEPAARKFVFAYFVRIEN
                     VGAVAARLLSRRWLIHDAGGDDTEVIGDGVIGEQPLLEPRGVHEYSSFSVLKSPAGHM
                     EGHYHFVRADGGHFDALIPRFILDATASTGPVH"
     gene            complement(1023..1328)
                     /locus_tag="VFU45_04055"
     CDS             complement(1023..1328)
                     /locus_tag="VFU45_04055"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEU5358268.1"
                     /translation="MATEFRSFADFYPFYLGEHQDPICRRLHFTGTLLVIGTLAAVLA
                     TRHWSLLWLMPVFGYGFAWVGHYVFEKNRPATFKHPLYSLAGDFVMFGDILRGKVSL"
     gene            complement(1437..1664)
                     /locus_tag="VFU45_04060"
     CDS             complement(1437..1664)
                     /locus_tag="VFU45_04060"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HEU5358269.1"
                     /translation="MTSKRLQVYATPDITVTFDPTVCRHSAHCIRSLPAVFDVRRKHW
                     IRPELASAEDVARVVAGCPSGALQVRAGTDG"
     gene            complement(1661..2230)
                     /locus_tag="VFU45_04065"
     CDS             complement(1661..2230)
                     /locus_tag="VFU45_04065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEU5358270.1"
                     /translation="MTPVRALALVGSLRTGSYNRALWRAALALAPEGLALEEGPIGDL
                     PLYNGDLHPPAHPDWPAPVGRLAAQVAAADALLFISPEYNYSIPGVLKNAIDWVSRVQ
                     PSPFHDKPAAVMGATNGMWGTTRMQYHLRQVGVFTNLHFLNRPEMLVAKAQDKFSAEG
                     DLTDEATRKVLVQFLTAFRDWSLRFRRPA"
     gene            complement(2237..2404)
                     /locus_tag="VFU45_04070"
     CDS             complement(2237..2404)
                     /locus_tag="VFU45_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358271.1"
                     /translation="MATKLDKAIKRELTLGDKTYTIVIAPEGIKITFKGGRKGVEHSW
                     PMLVAGLDQQD"
     gene            complement(2459..2929)
                     /locus_tag="VFU45_04075"
     CDS             complement(2459..2929)
                     /locus_tag="VFU45_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358272.1"
                     /translation="MPTSRPIAVLVAWDSSAGITRLSVTTHPGMNFLWIRRPRLTFYF
                     EAIGREVTAPPATVEFLVHSQSPQDIVGNRLSFTCDTGTRQAEIVPAFAEKPDILTIN
                     QMLLYRLPVAEFDTLASCAELAVAVGRIHAAFSPEQLAALRDLAGRMYPPGGVN"
     gene            complement(3090..>3281)
                     /locus_tag="VFU45_04080"
     CDS             complement(3090..>3281)
                     /locus_tag="VFU45_04080"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEU5358273.1"
                     /translation="VRSDAVLATAAYLGGLWRLAAAGAIIPAGWRDAMYDVVARHRHR
                     LGGRRCAVPTQDAVARFLD"
BASE COUNT          540 a         1107 c         1148 g          486 t
ORIGIN      
        1 ccaccaccca cttccccgcc tcccgccgat accgcagtcg gtcgtggagc cggctctccc
       61 tgccctgcca gaactcgaac gcctcgggcg tcacccggaa gccgccccag tgcggtggga
      121 gcggcgggtc ctcgccgtag cgatgggcag cctgggcggc ggcggtctcg atcgcatcgc
      181 ggctctcgac gacggcgctc tggtgcgacg cccaggcgcc gagccggctg ccgagtgggc
      241 gcgaacggaa gtaggcctcc gactcggcgt cggtggtgcg gctcaccgcc cccgtcaccc
      301 gtacctggcg ctcgagctcc ggccaccaga agaggagcgc cgcgtgcggg ttcaccgcca
      361 actcgctccc ctttccactc cgccggtcgg tgaagaagac gaatccgcgc ttgtcgaacc
      421 ccttgaggag caccatccgc accgatggtg cgccgtgctc gtcggcggtg gcgagcgcca
      481 tggcactcgg gtcgagcgcc tctgcggcac gcgcctcggc gaaccagcgc tcgaaggccg
      541 cgagcggatc gggacccagg tcggccaggt cgaggcgggc gcgcttgtac tcgcgccgca
      601 ggtcggcaag gtcggccatc agtgcaccgg accggtggag gcggtggcgt cgaggatgaa
      661 gcgggggatc agggcgtcga agtgcccgcc atcggcgcgg acgaagtggt agtggccttc
      721 catgtgcccc gccggcgact tgagcaccga gaagctgctg tattcgtgga cgccgcgggg
      781 ctcaaggagc ggctgctcgc cgatgacccc gtcgccgatg acctcggtgt cgtctccgcc
      841 ggcgtcgtgg atgagccagc ggcgcgagag gaggcgcgcc gccacggcgc ccacgttctc
      901 gatccggacg aagtacgcaa agacgaactt ccgcgccgcg ggctccgact gctccggcag
      961 gtacgccggc cgcacggtga tgcggatgcc gtcggtctcg cggtaataga aaggcaacgg
     1021 cgtcacagcg agaccttccc gcgcaaaatg tcaccgaaca tcacgaaatc ccctgccagg
     1081 ctgtagagcg ggtgcttgaa ggtggcgggg cggttcttct cgaacacata gtgccccacc
     1141 cacgcaaagc cgtagccgaa gaccggcatc agccagagca ggctccagtg ccgcgtggcg
     1201 agcaccgccg cgagcgtgcc gatgaccagg agcgtcccgg tgaaatgcag ccggcggcag
     1261 atcgggtcct ggtgctcgcc caggtagaag ggatagaagt cggcgaagga gcggaattcg
     1321 gtggccatcg gagctcctcg atcggtcaaa agtcgttggt cgttggtcat tggtcggtcg
     1381 atggtcgttg gctcgtggat aacgtctaat gactatcgac caatgactga cgactatcag
     1441 ccatccgtcc ccgcccgaac ctggagcgcc cccgacgggc agccggctac gacgcgggcc
     1501 acgtcttccg ccgaggcgag ttcggggcgg atccagtgct tgcgccggac gtcgaagacc
     1561 gccgggaggc tccggatgca gtgcgccgag tgccggcaga ccgtcgggtc gaaggtgacg
     1621 gtgatgtccg gcgtggcgta gacctgcagg cgcttcgacg tcatgccggc cgccggaagc
     1681 ggagcgacca gtcgcggaac gcggtcagga actgcaccag caccttccgc gtcgcctcgt
     1741 cggtgaggtc accctcggcg gagaacttgt cctgcgcctt ggccaccagc atctcggggc
     1801 ggttcaggaa gtggaggttg gtgaagacgc ccacctggcg caggtggtac tgcatccgcg
     1861 tcgtccccca catcccgttg gtggcgccca tcaccgcggc gggcttgtcg tggaacggcg
     1921 agggctggac ccgggagacc cagtcgatcg cgttcttgag cacgccgggg atggaatagt
     1981 tgtattcagg agagatgaac agcagcgcat cggccgccgc cacctgggcg gcgagccgtc
     2041 ccaccggcgc cggccagtcg ggatgggccg gcgggtggag gtcgccgttg tagaggggga
     2101 ggtcgccgat cgggccctcc tcaagcgcca agccctccgg cgcgagcgcc agggcggcgc
     2161 gccagagggc cctgttgtac gaacccgtcc gcaggctccc gacgagggcc agggcccgca
     2221 ccggcgtcac ggggcgtcag tcctgctgat cgagtccggc cacgagcatc ggccacgaat
     2281 gctcgacgcc cttccgcccg cccttgaacg tgatcttgat cccctcgggc gcgatcacga
     2341 tcgtgtaggt cttgtcgccg agggtgagct cccgcttgat cgccttgtcg agcttggtcg
     2401 ccacggtggt gctcctgagt gagtgatgaa aggatcgccg gcggtcaggg ccgcccgatc
     2461 agttgacacc gccgggaggg tacatccgcc cggccaggtc gcgcaatgcc gccagttgct
     2521 cgggcgaaaa tgccgcgtgg atccggccta ccgccactgc cagctcggcg cacgacgcga
     2581 gagtgtcgaa ctccgccacg ggcaggcgat ataggagcat ctggttgatc gtgaggatgt
     2641 ccggcttctc ggcgaatgcc ggtacgatct ccgcctgtcg ggtcccggta tcgcaggtga
     2701 acgacaggcg attgcccacg atgtcctgcg gcgactggga gtggacgagg aactccacgg
     2761 tcgccggtgg cgccgtcacc tcgcggccga tcgcctcgaa gtagaaagtg agccgtggac
     2821 gccggatcca gagaaagttc atccctggat gcgtggtcac cgacaggcgc gtgatcccgg
     2881 cgctcgaatc ccaggccacc agcaccgcga tcggacggct ggtcggcatc ggcgcggcga
     2941 accgcggtgg cgtcggagcc ggcgcggcca ctccggcgtc atcgctggag accggcggca
     3001 agatcgcagg cagtgcgcaa ccgaggcagc agcccgccag catcgatacg acccaaggat
     3061 ttcgcagccg gcctccgctc gatggtgtat cagtcgagga accgcgctac cgcatcctgc
     3121 gtcggcaccg cacacctccg gccgccaagc cgatgccggt gacgggccac cacatcatac
     3181 atcgcgtccc gccatccggc cggtatgatg gcaccggcgg ccgccagacg ccagagcccg
     3241 ccgagatacg ccgccgtggc gagcaccgca tccgaccgta c
//
LOCUS       DASUAP010000163         4705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_86575, whole genome shotgun sequence.
ACCESSION   DASUAP010000163 DASUAP010000000
VERSION     DASUAP010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86575"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFU45_04085"
     CDS             complement(<1..693)
                     /locus_tag="VFU45_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358274.1"
                     /translation="MRVPFTLLLVLSLATRHSPLVTSLSAQTPAQRDSIETFRDSLAP
                     ISDTVALRALEARYIEIAKQDRNDPMRHLRLGFIAMRLGAAGRKDSYDDAAGEFEWAT
                     QVAPKEPYGWYGLGLAENAIGDASFTLVAGIQAMFGKDHLTRAAINYAKAAEVDPSFV
                     RGLLELGNTALEQRMNIKLDASLLAFRRAAATSGGHNPQVLLLRGRVERLVGDADSAL
                     VAFREYTGSPGAD"
     gene            complement(813..2804)
                     /locus_tag="VFU45_04090"
     CDS             complement(813..2804)
                     /locus_tag="VFU45_04090"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU5358275.1"
                     /translation="MTQITRRWCAASLILGVACAPHPSTPTVTPEPMAPLPAIARVDG
                     PLAIHVVYPSDKDLVDAGDSSFIFGSTGTGAATLTINGTPVRVWPNGAFLAWMAFPRD
                     SVMHFELAARAGADTASLDYVARRVQRFTPPTSGPWIDTTAVEPHGLAWWPRNEYLPI
                     HVRAAPGSTVRIILPGGRSIPLVADPHPVVAAWGTRAFDRDTLAVKLGTANDRFVGVV
                     RGLALGTPGPLFGDQAHEAHPAHEADASGSSASCAPCVSRASCACASIESVLGADTVR
                     VPWRVDIGLLDTLPVVTELNDDTAHTGTTDSLTVGREEPYGTYYWFFPTGTRAQVTGR
                     TNDEMRLRLADGVVAWVNADDAQPLPTGLPAPLGEVGSVRFVALSDRVQLRIPVGTRA
                     PFHVDEDASRLTLTLYGARGDVNWIQYGGVDPLIGGARWDAPAADQVRFTIDLTQPVW
                     GYRTRWDGTDLILEIRRPPVIDAAHPFQGRRIVIDPGHPPLGAKGPTGFREPEANLAV
                     ALEVRRLLEAEGATVLMTRTTDTAIDLYPRVHFADSVGAELLISIHNNAFPDGVDPFR
                     NAGTSSYYNQPQSLPWVRATQRALAARLGIRDLGFGRGDLALVRPTWMPAILTEGMFL
                     MVPEQENALRTADGQHRYAEAVVDGMRAFLQGRALVGVR"
     gene            2966..4621
                     /locus_tag="VFU45_04095"
     CDS             2966..4621
                     /locus_tag="VFU45_04095"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5358276.1"
                     /translation="MKFCPVCGTEYADDVKFCPRDGQTLKSANPSGDLIGQVIADRYH
                     ITKKLGEGGMGAVYLGEHVKMGRKSAIKVMTGSMSNDPDAISRFNREANNASRINHPN
                     VCAIYDFGETPDGIIYLAMEFIEGKSLSGLLKETGPLPLNRATAILRQTADGLEVAHE
                     LGIVHRDLKPDNIMIATQAGKDLVKVVDFGIAKAVGGEDTGQKVTKTGLVVGTPEYMS
                     PEQLSGDKLDGRSDIYSLALVYYRMITGALPFQAETSQETMIKRLTDDPMPLSEALPT
                     GHFPAGLQPVMDKALARYPNDRYASAAQFATDVEKALAGMSAAEQGATMVVGADQFKT
                     MVAGAAPKDMPKTRVASAADKGGAPAAAPSKSKLPLLVGAAAVVVAGVGFALKGTLFP
                     AKPGGAAGQPDSGVVARHDSGTAGQREGGKAPSVAAPQLNQGTAANSAAPQHNAVTPP
                     AAAPARPKVDEPAIMSELSDLQDAVLNDSTRRPTAIQKGTDYYGRSELSAKARAEAAA
                     TVGFTYMQEQQFTQARLWFERAVKLDSTKKTFLEGLDRVSHAN"
BASE COUNT          757 a         1549 c         1772 g          627 t
ORIGIN      
        1 gtcggcgccg ggcgagccgg tgtactcacg gaaggccacc agcgcggagt cggcatcgcc
       61 caccaggcgt tccacccgcc cgcgcaggag gagcacctgc gggttgtggc cccccgacgt
      121 ggccgcggcg cggcggaagg caaggagcga cgcgtcgagc ttgatgttca tccgctgctc
      181 gagcgcggtg ttgccgagct ccagcagtcc gcgcacgaag ctggggtcca cctcggcggc
      241 cttggcgtaa ttgatggcgg cgcgggtcag gtggtccttg ccgaacatcg cctggatgcc
      301 ggccacgagg gtgaagctcg cgtcgccgat ggcgttctcg gcgagcccga ggccgtacca
      361 gccgtacggc tccttcggcg ccacctgggt ggcccactcg aactcccccg cggcgtcgtc
      421 gtacgagtcc ttccgccccg ctgcaccgag ccgcatcgcg atgaacccaa ggcgcaggtg
      481 gcgcatcggg tcgttgcggt cctgcttggc gatctcgatg tagcgggcct cgagcgcgcg
      541 gagcgccacg gtatcgctga tcggcgccag ggaatcgcgg aacgtctcga tggaatcgcg
      601 ctgggcaggg gtctgcgcag acagcgatgt gacgagtggc gagtggcggg tggcgaggga
      661 caggacgagg agaagcgtga agggaacgcg catcgccacg gctccgggtc gggctccggg
      721 cgcggcgatc gtcggttgct ggcgggaacg ggtcgtggga ccgagcggca tacggtgcat
      781 tggggacgcc ccgggggaag ttccgtgcac gcctagcgta cccccaccag cgcgcgcccc
      841 tggagaaacg cccgcatccc gtccaccacg gcctcggcgt atcggtgctg gccgtcggcg
      901 gtgcggaggg cattctcctg ctccggcacc atgaggaaca tcccctcggt caggatcgcc
      961 ggcatccacg tcggccggac cagcgccagg tcgccgcgcc cgaaacccag gtcgcggatg
     1021 ccgagccgtg ccgccagcgc gcgctgggtg gcgcgcaccc agggaaggct ctgcggctgg
     1081 ttgtagtaac tgctggtgcc ggcgttccgg aacgggtcga cgccgtcggg gaaggcgttg
     1141 ttatggatcg agatcaggag ctcggcgccc acgctgtccg caaagtggac ccgggggtag
     1201 aggtcgatgg cggtgtcggt ggtgcgcgtc atcagcaccg tggcaccctc ggcctcgagc
     1261 agccggcgca cttcgagcgc cacggcgagg ttggcctcgg gctcgcggaa gccggtgggg
     1321 cccttggcgc cgaggggcgg gtgccccggg tcgatcacga tgcggcggcc ctggaagggg
     1381 tgcgcggcgt cgatgaccgg cgggcggcgg atctcgagga tcaggtcggt cccgtcccag
     1441 cgggtgcgat agccccagac cggctgggtc aggtcgatcg tgaagcgcac ctggtcggcg
     1501 gcgggggcat cccaccgggc cccgccgatg aggggatcga ccccgccgta ctggatccag
     1561 ttgacgtcgc cgcgggcgcc gtagagggtg agcgtgaggc gactggcatc ctcatcgacg
     1621 tggaacggcg cccgggtgcc gacggggatc cggagctgga cccgatcgga cagcgccacg
     1681 aaccggaccg agcccacctc gccgagcggc gcgggaaggc cggtggggag cggctgggcg
     1741 tcgtccgcgt tgacccacgc cacgacgcca tcggccaggc ggaggcgcat ctcgtcgttc
     1801 gtgcgaccgg tgacctgcgc ccgcgttccc gtcgggaaga accagtagta ggtgccgtac
     1861 ggctcctcgc gcccgacggt gaggctgtcg gtggtgccgg tgtgggcggt gtcgtcgttg
     1921 agctcggtga cgaccggcag ggtgtcgagg agcccgatgt cgacgcgcca cgggacgcgg
     1981 acggtgtcgg caccgaggac ggattcgatg gaggcgcatg cgcatgaggc gcgtgagacg
     2041 catggggcgc atgaagcgct cgagccggac gcatccgcct catgcgccgg atgcgcctca
     2101 tgcgcctggt ccccgaacaa cggccccggc gtgccgagcg cgagcccgcg caccacgccg
     2161 acgaagcggt cgttggcggt gccgagcttg acggcgaggg tgtcgcggtc gaaggcgcgg
     2221 gtgccccagg ctgcgaccac ggggtgcgga tcggccacga gcgggatcga acgtccaccg
     2281 gggaggatga tccggaccgt agagccgggg gcggcgcgga cgtggatcgg gaggtattcg
     2341 ttgcgcggcc accaggcgag gccgtggggc tcgaccgccg tggtgtcgat ccaggggccg
     2401 gacgtcgggg gggtgaatcg ctggacccgg cgggcgacgt agtcgagcga tgccgtatcg
     2461 gcgccggccc gcgccgcgag ctcgaagtgc atcaccgagt cgcgggggaa cgccatccat
     2521 gccaggaagg cgccgttggg ccagacccgg acgggggtgc cgttgatggt gagcgtggcg
     2581 gcaccagtgc cggtcgaacc gaagatgaag ctcgagtcgc cggcgtcgac cagatccttg
     2641 tcggagggat agacgacgtg gatggcgagg ggaccgtcca cccgcgcgat cgcggggagg
     2701 ggggccatcg gctccggggt cacagtcggt gtggacggat gcggcgcaca cgccacaccg
     2761 aggatcaggg acgcggcgca ccaccggcgg gtgatctggg tcataggcgc aaggtactct
     2821 ctggcaccaa cttacggcaa taacgcggtt gacgctcccg ccggcggccg gtagatttgg
     2881 cggtcaggcg ccggggaagg attgcccggc ggcgttgcat cctgctctac cccgcccaga
     2941 ccacgccttc acgttcggag tcggcttgaa gttctgtccg gtgtgcggta ccgaatacgc
     3001 ggacgacgtc aagttctgcc cccgggacgg ccagaccctc aagtcggcca acccgtcggg
     3061 cgacctgatc ggccaggtca tcgccgaccg ctaccacatc accaagaagc tcggcgaggg
     3121 cgggatgggg gcggtctacc tcggcgagca cgtgaagatg gggcgcaaga gcgccatcaa
     3181 ggtgatgacc gggtcgatgt ccaacgaccc cgacgccatc tcgcgcttca accgcgaggc
     3241 caacaacgcc tcccggatca accaccccaa cgtctgcgcc atctacgact tcggcgagac
     3301 acccgacggg atcatctacc tcgcgatgga gttcatcgag gggaagtcgc tctcggggct
     3361 cctgaaggag acggggccgc tcccgctcaa ccgcgccacc gccatcctgc gccagaccgc
     3421 cgacggcctc gaagtggccc acgagctggg catcgtccac cgcgacctga agcccgacaa
     3481 catcatgatc gcgacccagg ccgggaagga cctggtcaag gtcgtggact tcgggatcgc
     3541 caaggcggtc gggggcgagg acaccggcca gaaggtgacc aagaccggcc tcgtggtggg
     3601 gacgccggag tacatgagcc cggagcagct ctccggcgac aagctcgacg gccggagcga
     3661 catctactcg ctggcgctcg tgtattaccg gatgatcacc ggcgcgctcc ccttccaggc
     3721 ggagacgagc caggagacga tgatcaagcg gctgacggac gacccgatgc cgctctccga
     3781 ggcgctcccg accgggcact tcccggccgg actccagccg gtcatggaca aggcgctggc
     3841 ccgctatccc aacgaccgct acgcctcggc ggcgcagttc gccaccgacg tggagaaggc
     3901 cctcgcgggg atgtcggcgg cggaacaggg cgccacgatg gtggtgggcg ccgaccagtt
     3961 caagacgatg gtggcgggtg ccgcgccgaa ggacatgccg aagacccgcg ttgcgagtgc
     4021 tgcggacaag ggcggcgccc cggcggcggc gccgtcgaag agcaagctgc cgctcctggt
     4081 gggggccgcg gcggtggtgg tggcgggggt cggctttgcg ctcaagggga cgctcttccc
     4141 ggccaagccg ggcggggcgg ctggacaacc ggacagcggg gtcgtggcgc ggcatgacag
     4201 cgggacagcg gggcagcggg agggtggaaa ggcgccctcg gtcgctgcgc cgcagctcaa
     4261 ccaggggacc gcggccaact cggcggcgcc gcagcacaac gccgtgacgc cgccggcggc
     4321 cgcgcccgcg cggccgaagg tggacgaacc ggcgatcatg tccgaactct cggacctgca
     4381 ggacgcggtg ctcaacgatt ccaccaggcg gccgacggcg atccagaagg gtaccgacta
     4441 ctacgggcgg tccgagctca gcgccaaggc gcgggccgag gcggccgcca ccgtcggctt
     4501 cacgtacatg caggaacagc aattcaccca ggcgcgcctg tggttcgagc gcgccgtgaa
     4561 gctggactcg acgaagaaga ccttcctcga gggcctcgac cgagtgtccc acgccaattg
     4621 aggaccgcgt gacccccaaa cccgtccgcg tctccgtctt cggcaagacc gacgtcggcc
     4681 gcacccgcga ccacaacgag gacac
//
LOCUS       DASUAP010000164         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_726974, whole genome shotgun sequence.
ACCESSION   DASUAP010000164 DASUAP010000000
VERSION     DASUAP010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726974"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="VFU45_04100"
     CDS             complement(<1..1697)
                     /locus_tag="VFU45_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5358277.1"
                     /translation="MAIAASRQRMEEAPPRKELRGVVERITYQNAENGYTVARLAPER
                     PEGEARAAQGADRLVTLVGTLADLTPGEAIVAHGWWQNNPKHGWQFQVLEYRTALPAT
                     TQGMRRYLGSGLVKGVGPVMAGRIVDAFGDDTFAVIDATPARLTEVPGIGKVRAGRIA
                     ATWVEQRHIREVMTALQGYGVSTSLAVRIYKRFGDDSGRVVSQEPYRLAREVWGIGFK
                     TADTIAQAVGIAPDAPERLQAGVLHALGAAGDAGHTLLPERELVETAADLLGVEAARL
                     SASVEALLCTGDLVAASREGVTLRLVALAPFARAESGLASRLVALAAAAKDARVARTF
                     AATDWAAAFGWLATKHGLTLAPEQEAAVRMALTSPVSILTGGPGTGKTHTLRAILTLA
                     RAKRLRPLLAAPTGRAAKRMQEATGLDAGTLHRTLELRPGGKAGRGSDNPLDADLVVV
                     DEVSMLDALLANQLAKAVPPGSHLLLVGDPDQLPSVGAGDVLADLLRSGRFPVTRLTH
                     IFRQGAGSGIALNAARINAGQLPRFGGQIQDCFFLPGADPVATAETAVEIVAKRLPRR
                     "
     gene            complement(1912..>2193)
                     /locus_tag="VFU45_04105"
     CDS             complement(1912..>2193)
                     /locus_tag="VFU45_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5358278.1"
                     /translation="ALRKTWGLPRAQPAPPPPPSADDRRYSVDEAAVVLGVAAGTVFT
                     WVRRGRLRGAQVAKGQPWTIALTPDEIAALRARAARDRPRVRQLSMEVV"
BASE COUNT          294 a          791 c          799 g          309 t
ORIGIN      
        1 cgtcgtggga gtcgcttcgc cacgatctcg accgcggttt ctgcggtcgc caccgggtcc
       61 gccccgggca ggaagaagca gtcctggatc tgcccaccga agcgcggcag ctgcccggcg
      121 ttgatccggg ccgcgttcag cgcgatgccc gaacccgcgc cctgacggaa gatatgcgtg
      181 agcctcgtga ccgggaagcg cccggagcgc aggaggtccg cgaggacatc gccggctccg
      241 acgctgggga gttggtcggg gtcgcccacc agcaagagat ggctccccgg cgggacagcc
      301 ttggccagct ggttggcgag gagtgcgtcg agcatgctca cctcgtcgac caccaccagg
      361 tcggcgtcga gcgggttgtc ggaacctcgc ccggctttcc cgccggggcg cagttcgagc
      421 gtgcggtgga gcgtgcccgc gtccagcccg gtcgcctcct gcatccgctt ggctgcgcgt
      481 cccgtcggcg cggcaaggag agggcgtaaa cgtttggccc gcgcgagggt gaggatcgcc
      541 cgcaaggtat gggtcttgcc ggtcccgggc ccgccggtga ggatgctgac gggtgaggtc
      601 agggccatcc gcaccgcggc ctcctgctcc ggcgcgaggg tcagcccgtg cttcgtcgcg
      661 agccagccga acgcggcggc ccagtcggtc gccgcgaagg tgcgcgccac gcgcgcatcc
      721 ttggcggccg cagccagggc gaccaggcgc gaggccagcc ccgactccgc ccgggcgaaa
      781 ggcgcgagtg ccactagccg cagggtgacg ccctcccggc tcgccgccac gagatcgccg
      841 gtgcacagca gggcttccac cgacgcactc agacgcgccg cctcgacgcc cagcaggtcg
      901 gccgccgtct cgaccagctc gcgctcgggg agcagggtgt gccccgcgtc gccagccgcc
      961 cccagcgcgt gcagcacacc cgcttgcagg cgctcggggg catccggggc gatgcccacc
     1021 gcctgggcga tcgtgtcggc ggttttgaag ccgatccccc agacctcgcg ggccaggcga
     1081 tagggctcct ggctgacgac tcgtccgctg tcgtccccga agcgcttgta gatgcgcacg
     1141 gcgagcgagg tggagacgcc gtagccctgc agcgcggtca tcacctcgcg gatgtgccgc
     1201 tgctccaccc aggtggcggc gatgcggccc gcccgcacct tcccgatgcc ggggacctct
     1261 gtgagccgcg cgggcgtggc gtcgatgacg gcgaaggtat cgtccccgaa ggcgtcaacg
     1321 atgcgcccag ccatcaccgg tccgacgccc ttcaccaggc cgctccccag gtagcggcgc
     1381 atcccctggg tcgtcgccgg cagggcggtg cggtactcaa gcacctggaa ctgccagccg
     1441 tgcttggggt tattctgcca ccagccgtgg gcgacgatcg cctcgccggg cgtgaggtcg
     1501 gcgagggtgc cgacgagggt gaccaggcgg tccgcgccct gggcggcgcg cgcctcgccc
     1561 tcgggccgct cgggggcgag ccgggccacc gtgtagccgt tctccgcgtt ctggtaggtg
     1621 atgcgctcaa ccacgccccg caactccttg cggggtgggg cctcctccat gcgctggcgg
     1681 ctcgcggcga tggccatcga cagcgctcct cgctcaacac tgatcatacc gcaagacagc
     1741 cccaccgggc tcatcgacag ggtagcgtcg gggcggccac cgggcggtgc cggggcagcc
     1801 ggcttcgtta ccctgttcgc gatggcgcga gcggcgctgg acggctgcaa cgcgcgggag
     1861 gaggatacga tgatcaacga ctacatggct cggtggctcg aagtgaaggt ttcatactac
     1921 ctccatgctc aattgcctga ctcgtggccg gtcccgtgcg gcgcgagcgc gcagggcggc
     1981 gatctcgtcg ggggtcaggg cgatcgtcca gggttgccct ttggcgacct gggcaccgcg
     2041 caaccggccg cgccgcaccc aggtgaagac cgtgcccgcg gcgaccccca gcaccacggc
     2101 ggcctcgtcg acgctgtacc gccggtcatc ggccgacggc gggggcggcg gggccggctg
     2161 ggcccgcggc agcccccagg tcttgcgcaa cgc
//
LOCUS       DASUAP010000165         5305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_72895, whole genome shotgun sequence.
ACCESSION   DASUAP010000165 DASUAP010000000
VERSION     DASUAP010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72895"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1254)
                     /locus_tag="VFU45_04110"
     CDS             complement(139..1254)
                     /locus_tag="VFU45_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU5358279.1"
                     /translation="MTHDPQRATRTVLIAGGGTGGHLIPALAIAKALRETGAGIDPVL
                     VGAERGVESWLLPERDFPYHLLPIRPLFRQRIWRNAALPWSLVRTVAGLRRVFRERRP
                     ALVIGTGGYASGPAVWWASRRGIPVAIQEQNAWPGLATRLLAGRVDHIYLGAPEARAR
                     LRPGRRTTVFDTGNPIAPPDPARRAGAQKRFGLTGGEQVLLVTGASQGAVPINRAVAG
                     WLDAGGATDMVVLWATGKGSYAEFRHYHRPPAVQVFDFLDPIADAYAVADLAVARGGM
                     MTGAELCAWGIPSIIVPLPTAADDHQTANAMALELAGAARHLPQAELSATTLGRVIDE
                     LMADGVARAEMAARAKERGRPRAAADIVSHCLTLLGP"
     gene            complement(1331..2485)
                     /locus_tag="VFU45_04115"
     CDS             complement(1331..2485)
                     /locus_tag="VFU45_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEU5358280.1"
                     /translation="MSAPAIRHPGEQRWETRLLAVVTATLVVFGIASSYGAASLLTNR
                     AGDNIGMSFALKQLSGAVAGGILLLIASRMDYHRWRHFAWPLLGFTVLALLIPLLPFT
                     HRFAPSIHGARRWVDIGPVNFQPSEFARLAVVVWAATLAAKKGDQLREFKRGVLPFVI
                     VIGFVSLLILLEPNLSMATLVAFIAGVVLFTAGAKIGHFLLLGGVGTVLVWHKIVDTQ
                     YRAARLLTFLNRDDASTQASFQITQSLVGMGSGRIFGVGFGAGQQKLGYLPFGYSDFL
                     FSVIGEEWGFLGVCVVVLLFGLFVWLGFRIARTAPDAFGQYLAAGITSAVGITAFLHM
                     AVTLGMMPTTGLTLPFMSYGRSSLVITLLATGILLSVGRQRGKVPAGGKR"
     gene            complement(2482..3828)
                     /gene="murD"
                     /locus_tag="VFU45_04120"
     CDS             complement(2482..3828)
                     /gene="murD"
                     /locus_tag="VFU45_04120"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU5358281.1"
                     /translation="MIDRWLVDGREVAVVGLGRSGVAATRLLRSRGVPVYASDAGPGG
                     DATTLDQLRAAGATVGTGGHDLARIAKAQAVVVSPGVPPAAPPLQAAARAGVPVVAEA
                     GLGLAELPGTRTICITGTNGKTTTTALTGHLLAAVGKRAGTAGNIGRPVSEVALERPA
                     PDWLAVELSSFQLHDMPQLHPTIGALTNLAPDHLDRYARLEDYYADKARLFRNAGPGS
                     VWIVNGDDGAVLEMVRGVAGTVLRFSLAGRADAWFDRAAGTLMLHDAPVLARGDLALL
                     GDHNVANALLAALATAQAGGDLARIGAGLRSFRALPHRMEPVREVGGVLWINDSKATN
                     VASTDVAVRAMARPFVLLLGGRHKGEPYTRLAEGFQGRCRGVVAYGESGALVERDLRD
                     RVTVVRGGDFPDVLARARALVRPGDAVLLSPACSSYDMFTNYEERGARFRAAVEAM"
     gene            complement(3828..4955)
                     /gene="mraY"
                     /locus_tag="VFU45_04125"
     CDS             complement(3828..4955)
                     /gene="mraY"
                     /locus_tag="VFU45_04125"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU5358282.1"
                     /translation="MFYHLLAPLGSKYLLFNLFNYLSFRAAGATVTALVMAFIVGPGI
                     IRRLREHKVGQVIRAEGPASHQGKRGTPTMGGVIILLCTIVPTLLWAPLNNRFVLVAM
                     GATIWMGAIGFVDDYLKIVQGRSRGLVAKWKLAGQVTFGVALGITLLLWPAVPPETIP
                     ASATTVPVFKYVVVVFAPLLYVVFVTGVVTGFSNAVNLTDGLDGLATGLTAIAAGAFA
                     LFAYIFGRVDVTQYLYLFYLPGAGELAVFCAALMGAALGFLWFNAHPAEVFMGDTGSL
                     AIGGAFGTVAILLKAEFLLVILGGVFVAETVSVMIQTSTYKWFKRTRGKEYADAHRVF
                     RMAPLHHHFEKLGWQETTVVTRFWILGLFCALLALATLKVR"
     gene            complement(4962..>5305)
                     /locus_tag="VFU45_04130"
     CDS             complement(4962..>5305)
                     /locus_tag="VFU45_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358283.1"
                     /translation="TARAMRPGRRLVFVAGTMKELGPDSARYHRDVAEALVALGPDLL
                     AAVGEFVPALEPYRGALGDRLLVAPDAPALGPLLKARLRGDELVVLKASRGAALERIL
                     PYLGLTPATSH"
BASE COUNT          847 a         1886 c         1908 g          664 t
ORIGIN      
        1 ggcgggcaat gagggccagc gcactcatgc cggcgccgcc gatgcccatg aagtgcaccg
       61 ggcgggggtc ttcgggggag aacagatcca ttggttggac agctaattta agccctattc
      121 agcagttgcg aaagagtgtc agggccccaa aagcgtcaaa caatgcgaca caatgtccgc
      181 tgcggcgcgg ggtcgacccc gttccttcgc ccgcgcggcc atttcggccc gcgccacccc
      241 gtcggccatc agttcgtcga tcacccgtcc cagcgtcgtc gcgctcagct ccgcctgcgg
      301 caggtgccgc gccgccccgg caagctcgag cgccatcgca ttcgcagtct ggtggtcgtc
      361 ggccgcggtc gggagcggga cgatgatcga ggggatgccc cacgcgcaga gctccgcccc
      421 ggtcatcatc ccgccacgcg ccacggcgag gtcggccacc gcatacgcgt cggcgatcgg
      481 gtcgaggaag tcgaacacct gcaccgccgg cggccggtgg tagtggcgga actccgcgta
      541 actccccttg cccgtggccc acaggaccac catgtcggtg gcgccgccgg catcgagcca
      601 gccggcgacc gcccggttga tcggcaccgc cccctggctg gcaccggtca cgagcagcac
      661 ctgctccccg ccggtgaggc cgaagcgctt ctgcgcgccc gcccgtcgtg ccgggtcggg
      721 cggggcgatg ggattcccgg tgtcgaagac ggtagtgcgg cgtccgggcc ggagccgggc
      781 ccgggcctcg ggggcgccca gatagatgtg atcgacccgg ccggcgagga gccgggtggc
      841 gagcccgggc caggcgttct gctcctggat cgcgaccggg atcccgcggc gcgacgccca
      901 ccataccgca ggacccgacg cgtagccacc ggtgccgatc acgagcgcgg gccggcgctc
      961 ccggaacacc cgccgcaacc cggccaccgt gcgcaccagg ctccacggga gcgcggcatt
     1021 ccgccagatc cgctggcgga aaagcggccg gatggggagg agatggtacg ggaagtcccg
     1081 ctcgggcagg agccacgact cgacgccccg ctcggcaccg accagcaccg gatcgatgcc
     1141 ggcgcccgtc tcacgcagcg ccttggcgat ggcgagcgcc gggatcaggt gcccgccagt
     1201 gccgccgccg gcgatgagga cggtgcgggt ggcgcgttgc gggtcgtggg tcacgggccg
     1261 tgggtcacgg gtcgcgggtc tttggtcgtt ggtcacgaat cttcgcacgc ccccgttcac
     1321 cgttgactgt tcaccgtttc ccacccgccg gcaccttccc ccgctggcgc ccgacgctca
     1381 gcaggatgcc ggtggcgagg agggtgatga cgaggctcga gcggccgtag gacatgaacg
     1441 ggagcgtgag gccggtggtc ggcatcatgc cgagggtcac ggccatgtgg aggaaggcgg
     1501 tgatcccgac cgcgctggtg atgcccgcgg cgaggtactg gccgaaggcg tccggcgcgg
     1561 tccgcgcgat gcggaatccg agccagacga aaaggccgaa gaggagcacc acgacgcaca
     1621 cgccgaggaa gccccactcc tcgccgatca cgctgaacag gaagtcggag tacccgaacg
     1681 gcaggtagcc gagcttctgc tgtcccgccc cgaagccgac gccgaagatg cggcccgagc
     1741 ccatccccac cagcgactgg gtgatctgga agctcgcctg ggtggaggcg tcgtcgcggt
     1801 tgaggaaggt gaggagtcgc gcggcgcggt actgggtgtc gacgatcttg tgccacacga
     1861 gcaccgtgcc gacgccgccg agcaggagga agtgcccgat cttggcgccg gccgtgaaga
     1921 gcacgacgcc ggcgatgaac gccaccagcg tcgccatcga caggttgggc tcgagcagga
     1981 tgaggagcga cacgaacccg atcacgatca cgaacggcag caccccgcgc ttgaactcgc
     2041 ggagctggtc gcccttcttg gccgccaggg tggccgccca gacgacgacg gccaggcgcg
     2101 cgaactccga cggctggaag ttcaccgggc cgatgtccac ccagcggcgg gcgccgtgga
     2161 tgctgggcgc gaaccggtgg gtgaacggca gcagcgggat gaggagcgcc agcaccgtga
     2221 agccgaggag cggccaggcg aagtggcgcc agcggtgata gtccatccgc gacgcgatga
     2281 ggagcaggat ccccccggcc accgcgccgg agagctgctt gagcgcgaac gacatcccga
     2341 tgttgtcgcc agcccggttg gtcaggaggc tcgccgcgcc gtagctcgac gcgatcccga
     2401 agacgacgag ggtcgccgtc acgacggcga gcagccgcgt ctcccagcgc tgctcgccgg
     2461 ggtggcggat cgccggcgcg ctcacatcgc ctccacggcg gcgcggaagc gcgccccgcg
     2521 ctcctcgtag ttggtgaaca tgtcgtagct cgaacacgcg ggcgagagga ggaccgcatc
     2581 acccggtcgg accagggcgc gcgcccgggc cagcacgtcg ggaaagtccc cgccccggac
     2641 caccgtgacc cggtcgcgga ggtcgcgctc gaccagcgct ccggactcgc cgtaggccac
     2701 cacgccccgg caccgcccct ggaacccctc ggccagccgg gtgtagggtt cgcccttgtg
     2761 gcggccgccc aggagcagca cgaacggccg cgccatcgcc cgcacggcga cgtcggtgga
     2821 cgcgacgttg gtcgccttgg aatcgttgat ccagaggacg ccgccgacct cgcgcaccgg
     2881 ctccatccgg tgcggcagtg cgcggaagct ccggagtccg gcgccgatcc gcgccaaatc
     2941 gcccccggcc tgggcggtgg cgagcgccgc caggagcgcg ttggccacgt tgtggtcgcc
     3001 gaggagcgcc aggtcgcccc gggcaaggac gggggcatcg tgcagcatca gcgtcccggc
     3061 ggcgcggtcg aaccaggcgt cggcccgccc ggccagggaa aagcggagca ccgtgccggc
     3121 cacgccgcgg accatctcca ggacggcccc gtcgtcgccg ttgacgatcc agaccgatcc
     3181 cggccccgcg ttccggaaga gccgcgcctt gtcggcatag tagtcctcca gccgggcgta
     3241 gcggtccagg tggtcggggg cgaggttggt gagcgcgccg atcgtggggt ggagctgcgg
     3301 catgtcgtgc agctggaagc tcgacagctc gaccgcgagc cagtccggcg ccgggcgttc
     3361 cagcgccacc tcggagacgg ggcggccgat gttccccgcc gtgcccgcgc gcttgcccac
     3421 ggcggcgaga aggtgcccgg tgagcgcggt cgtcgtggtc ttcccgttcg tgccggtgat
     3481 gcagatggtc cgggtgccgg gcagctccgc caggccgagc cccgcctccg ccaccaccgg
     3541 caccccggcg cgcgccgcgg cctggagcgg cggcgcggcc ggcggtaccc ccggcgagac
     3601 gacgaccgcc tgcgccttcg cgatgcgcgc gaggtcgtgg ccgccggtcc cgaccgtggc
     3661 acccgccgcg cgcagctggt cgagcgtcgt ggcgtccccg cccggtccgg cgtcgctcgc
     3721 gtacaccggc acgccccgcg aacgcaggag ccgggtggcc gccacaccgc tccggcccag
     3781 ccccaccacg gcgacctcgc gtccgtccac cagccagcga tcgatcatca gcggaccttg
     3841 agcgtcgcga gggcgaggag cgcgcagaag aggccgagga tccagaagcg ggtcaccacg
     3901 gtggtctcct gccagccgag cttctcgaaa tgatggtgca acggcgccat ccggaagacc
     3961 cgatgcgcgt cggcgtattc cttaccgcgg gtccgcttga accacttgta cgtgctggtc
     4021 tggatcatca ccgacacggt ctccgccacg aacaccccgc ccaggatcac cagcaggaac
     4081 tcggccttga gcaggatggc cacggtgccg aaggcgccgc cgatggccag gctcccggtg
     4141 tcgcccatga acacctcggc ggggtgcgca ttgaaccaga ggaacccgag ggccgccccc
     4201 atcagcgccg cgcagaagac ggcgagctct cccgcgcccg gcaggtagaa cagatagagg
     4261 tactgggtga cgtccacccg gccgaagatg tacgcgaaga gcgcgaacgc ccccgctgcg
     4321 atggccgtga gccccgtggc caggccgtcg agcccgtcgg tcaggttgac ggcattggag
     4381 aaccccgtca cgacgccggt cacgaatacg acgtagagga gcggcgcgaa gaccacgacg
     4441 acgtacttga agaccggcac cgtcgtggct gaggccggga tcgtctccgg cgggaccgcc
     4501 ggccagagca ggagcgtgat cccgagggcc accccgaagg tgacctgccc cgccagcttc
     4561 cacttggcca ccagcccgcg cgagcgcccc tgcacgatct tgaggtagtc gtccacgaag
     4621 ccgatggcgc ccatccagat ggtggcgccc atcgccacca gcacgaagcg attgttgagc
     4681 ggcgcccaca ggagcgtggg cacgatcgtg cacagcagga tgatgacgcc gcccatcgtc
     4741 ggcgtgccgc gcttcccctg gtgactggcc gggccctcgg cgcggatgac ctggcccacc
     4801 ttgtgctccc gcaggcgccg gatgatcccc ggtcccacga tgaacgccat caccagcgcg
     4861 gtgacggtgg cgccggccgc ccggaagctc aggtagttga agaggttgaa caggaggtac
     4921 ttgctgccga ggggggcgag cagatggtag aacatcgtcc ctcagtgcga agtggccggg
     4981 gtgagaccga gatacggaag tatacgttcc agcgccgcgc cgcgggacgc cttgagcacc
     5041 accaattcat cgccccggag ccgggccttg aggaggggac cgagggcggg cgcatcgggc
     5101 gccaccagca gccggtcgcc gagcgcgccg cggtacggct cgagcgccgg cacgaactcc
     5161 cccacggcag cgagtaggtc cgggccgagc gccacgaggg cctcggccac gtcccggtgg
     5221 taacgtgccg aatccgggcc gagctccttc atcgtcccgg ccacgaagac caggcggcgc
     5281 cccgggcgca tcgcgcgggc ggtcg
//
LOCUS       DASUAP010000166         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_31890, whole genome shotgun sequence.
ACCESSION   DASUAP010000166 DASUAP010000000
VERSION     DASUAP010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31890"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFU45_04135"
     CDS             <1..477
                     /locus_tag="VFU45_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358284.1"
                     /translation="VHRGPTVALGYWKDPALSALTFRADPRAAGDGERVVFSGDLARQ
                     DADGFLYYVSRKDRVIKAMGYRVGPDEIVDVLHASGQVTDAEVVGEPDPTWGTRIVAY
                     VTLAPSGSLAALKAFCARELPRHMQPARYESRSALPLLPSGKHDLAALTGAGSAAR"
     gene            558..1808
                     /locus_tag="VFU45_04140"
     CDS             558..1808
                     /locus_tag="VFU45_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5358285.1"
                     /translation="MDKRSFIRSIGGGTLALLVGDALWARYARLPAAALAREEDFWAT
                     VRGQYRLTTDYINLENGYYSMQSQPVLEAFIGRVRELNFRASRYLRTDQFKDKDAVRD
                     RLAALAGCASEELIITRNTTESLDTVIAGFDWQAGDEAVMAAQDYGAMLDMFALQARR
                     HGMVNRIVSLPLDPASDEEIVRLYENAITPKTRLLMVCQMVNITGQILPVAKVADMAH
                     RHGVQVMVDGAHTFAHIDFKIPDLHGDYFGTSLHKWLGSPLGAGLLWVRRDRIGPLWP
                     IFANSGVTDEQIAKLNHTGTHPCHTDLAINDAIDYHLAIGIERKEARLRYLQQYWTSR
                     VRGMPGIVLNTPTDPARSCAIANVGIPKLTPAELATALFDRYKVWTVAIDGAGVRGTR
                     VTPHVFTSTAELDTLVRALKELAA"
     gene            complement(1789..2532)
                     /locus_tag="VFU45_04145"
     CDS             complement(1789..2532)
                     /locus_tag="VFU45_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5358286.1"
                     /translation="MRGFDVEHPRWLVPSLLLAATVAVIAPAGVAAQGIDGSHAIRCG
                     DSTYRYVLYVPHHDTPAPVLVLLHGAGDTPEPMVDAWRRLAGREGIVLVAPALPRVAG
                     FEALAPGVFRCMVDEVKRLTAVDPRRVYVFGHSMGGYLGYDAALLESRYFAAAAIHAM
                     GIAPDYDWIVGKAARKTPIAMYIGDRDQFVPLAGVRRTRDLLVAHGFPVHYVELAGHD
                     HDYYALSGRINTDAWKFLERQALSTPPAP"
     gene            2682..>3194
                     /locus_tag="VFU45_04150"
     CDS             2682..>3194
                     /locus_tag="VFU45_04150"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU5358287.1"
                     /translation="MAVTADLARTFHRLHAEGCFVSPNPWDVGSARLLAQLGFKALAT
                     TSSGLAWSLGTRDTRVPLGQVLEHCRAVAAAVDIPVSADFEGGFATAPEDVAANVAAA
                     LTTGIAGLSIEDSTGDPARPLHHLALAVERIRAARHAVDSSGTGALLTGRSEGFIVGR
                     PDLAETIRRLT"
BASE COUNT          498 a         1168 c         1075 g          453 t
ORIGIN      
        1 gtgcaccgcg gacccaccgt ggcgctcggc tactggaagg atcccgcgct cagcgcactc
       61 accttccggg ccgatccccg ggccgcgggc gatggcgaac gggtggtctt cagcggcgac
      121 ctggcccggc aggacgccga cggtttcctc tattatgtga gccggaagga ccgggtcatc
      181 aaggcgatgg gctaccgggt cggccccgat gagatcgtgg acgtgctgca cgcctcagga
      241 caggtgaccg atgccgaagt ggtcggcgag cccgatccca cgtgggggac gaggatcgtc
      301 gcctacgtga cgctcgcacc gtcgggaagc cttgccgcgc tcaaggcctt ctgcgcccgc
      361 gagctgccgc gccacatgca gccggcgcgg tacgagtcgc ggagcgcgct gccgctcctg
      421 ccgagcggca agcacgacct ggcggcgctg acgggagcgg ggtccgccgc gcgctgaccc
      481 tccggggctg gacggagcgc gtcggcggcg cgagcttggg gcatccccct cgtcccgttc
      541 cacccggtgg ccacgccatg gacaaacgca gcttcatccg cagcatcggg ggcggcacgc
      601 tcgccctgct ggtcggcgac gcgctctggg cccgatacgc ccgcctcccc gccgccgccc
      661 tggcgcggga agaggacttc tgggccaccg tccgcggcca gtaccgtctc accaccgact
      721 acatcaacct cgagaacggc tactactcga tgcagtcgca gccggtgctc gaggcgttca
      781 tcgggcgggt gcgcgagctc aacttccgcg cctcgcgcta cctccgcacc gaccagttca
      841 aggacaagga cgccgtgcgc gaccgcctgg ccgcgctcgc cgggtgcgcc agcgaggagc
      901 tcatcatcac ccgcaacacg accgagtcgc tcgacacggt catcgccggg ttcgactggc
      961 aggcggggga tgaggcggtg atggccgcgc aggactacgg ggccatgctc gacatgttcg
     1021 cgctgcaggc ccgccggcac gggatggtca accggatcgt ctccctcccg ctcgacccgg
     1081 cctcggacga ggagatcgtc cggctctacg agaacgccat caccccgaag acccggctgc
     1141 tgatggtgtg ccagatggtc aacatcaccg ggcagatcct gccggtcgcc aaggtggccg
     1201 acatggccca ccgccacggc gtccaggtga tggtcgatgg cgcgcacacc ttcgcgcaca
     1261 tcgacttcaa gatccccgac ctccacggcg actatttcgg cacctcgctc cacaagtggc
     1321 tcggctcgcc gctcggcgcc gggctcctct gggtgcgccg ggaccggatc ggcccgctct
     1381 ggcccatctt cgccaactcc ggcgtcaccg acgagcagat cgccaagctg aaccacaccg
     1441 gcacccatcc gtgccacacc gacctcgcca tcaacgacgc catcgactat cacctggcga
     1501 tcgggatcga gcggaaggag gcccggctcc gctacctgca gcagtactgg accagccggg
     1561 tgcgcggcat gccggggatc gtgctcaaca ccccgaccga cccggcgcgc tcctgcgcca
     1621 tcgccaacgt cggcatcccg aaactcaccc ccgcggaact cgcgacggcg ctcttcgacc
     1681 gctacaaggt gtggaccgtg gccatcgacg gcgccggcgt ccgcggcacg cgggtgacac
     1741 cgcacgtctt cacctcgacc gcggagctcg acaccctggt gcgggcgctc aaggagctgg
     1801 cggcgtagac agcgcctggc gttcgaggaa cttccacgcg tcggtattga tccggcccga
     1861 cagggcgtaa tagtcgtggt cgtggcccgc gagctccacg tagtgcaccg ggaagccgtg
     1921 ggccacaagg aggtcgcggg tgcgccggac cccggccaac gggacgaact ggtcgcggtc
     1981 gccgatgtac atggcgatcg gcgtctttcg cgccgccttg ccgacgatcc agtcgtagtc
     2041 gggcgcgatc cccatcgcgt ggatggcggc ggcggcgaag taccgggact cgaggagcgc
     2101 cgcatcgtat cccaggtacc cgcccatcga atgcccgaag acgtacaccc gccgcgggtc
     2161 caccgcggtg aggcgtttca cctcgtcgac catgcagcgg aacaccccgg gcgccaacgc
     2221 ctcgaatccc gccacccgcg ggagggccgg cgccacgagg acgatgccct cccggccggc
     2281 gaggcgccgc caggcatcga ccatcggttc cggcgtgtcg cccgcaccat gcaggaggac
     2341 gagcaccggc gccggcgtgt cgtggtgggg tacgtacagc acgtagcggt aggtgctgtc
     2401 gccgcagcgg atggcgtgcg agccatcgat gccctgggct gcgacaccgg cgggcgcgat
     2461 gaccgcgacc gtggccgcca gcagcaacga tgggacgagc caacggggat gttccacgtc
     2521 gaaccccctc atggtgtcgc acctggccaa tgcatacgac agtaccgcgc ggctgcgact
     2581 tcgcgcagat tcgtcgggag acgaacgggg ctccctggcc ggcacgtctg ccacgacccg
     2641 gctgaaggga gcgggctcct caaatacgga cggatgttcc tatggctgtc acggccgatc
     2701 ttgcgcgcac gttccaccgc ctccacgccg agggctgctt cgtaagcccg aacccgtggg
     2761 atgtcgggag tgcccgcctc ctggcgcagc tcgggttcaa ggccctcgcc acgacgagtt
     2821 ccgggctcgc ctggtcgctc ggcacccggg acacccgggt gccgctcggt caggtgcttg
     2881 agcactgccg cgccgttgcg gccgcggtcg acatcccggt gagcgccgac ttcgaaggtg
     2941 gcttcgcgac cgcaccggag gacgtggcgg ccaacgtcgc tgccgcgctc acgaccggga
     3001 tcgccgggct gtcgatcgaa gattcgaccg gcgaccccgc acgaccgctg caccacctgg
     3061 ctcttgcggt cgagcggatc cgggcggccc ggcacgccgt cgactcgagc ggtaccggcg
     3121 cgctcctcac cgggcgctcc gagggattca tcgtcggtcg acccgacctt gccgagacca
     3181 tccggcgact cact
//
LOCUS       DASUAP010000167         4968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_495106, whole genome shotgun sequence.
ACCESSION   DASUAP010000167 DASUAP010000000
VERSION     DASUAP010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495106"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFU45_04155"
     CDS             complement(<1..379)
                     /locus_tag="VFU45_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358288.1"
                     /translation="MTTRLFRWKAIIPLVLFLLVICLGWVLFADWLGRRAVIAAGTKM
                     TGAEVDLAAFHIHLLRGSVELDGLQLADAGDSTRNTFDADRMVFDLDPAALFEKKLVI
                     DQLAVTGARVGARRATPARTIPRA"
     gene            complement(384..905)
                     /locus_tag="VFU45_04160"
     CDS             complement(384..905)
                     /locus_tag="VFU45_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03546 family protein"
                     /protein_id="HEU5358289.1"
                     /translation="MLAILKLIQSIVKTLHSDGSPNQVAFGLALGAALGLTPLFSLTN
                     VAIFALLFLLNVSFGAGMLGWVISVPLGFALDPVFDRVGRALLIDSPGLTPVWTRLAN
                     TPLVPFTNFNNTVALGSLVSWLVLFVPMVLFFRWAVVRYRATIGQKVMGSRIYKAVTG
                     SQLYNYYRLFRPE"
     gene            complement(1053..1382)
                     /locus_tag="VFU45_04165"
     CDS             complement(1053..1382)
                     /locus_tag="VFU45_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5358290.1"
                     /translation="MDAYRDQYAQLFGNGKRATLLAISVDADTTLADWARESNFPFRF
                     LSDTAGAVGRLYGARNEKYHVDNRTLYVVGPDGRIAYVAAPFREIDPLAYRQLDSALT
                     AVTKPRK"
     gene            complement(1392..1631)
                     /locus_tag="VFU45_04170"
     CDS             complement(1392..1631)
                     /locus_tag="VFU45_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358291.1"
                     /translation="MNALRNYGLALSALLGAATVAAQQAPPAAPAPLAVGTVAPDFVL
                     PGATKDGLTAKPIRLSDLRDQTVVIAFFFQARTKG"
     gene            complement(1633..2892)
                     /locus_tag="VFU45_04175"
     CDS             complement(1633..2892)
                     /locus_tag="VFU45_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5358292.1"
                     /translation="MHPVLRAAIGLAGAATIAAATRPVAPPCDPGNAGLVLPPGFCAT
                     LFGEGLGSPRNLTVLPNGDVVAARQRGGAVLLRDTTGDGHADVVNYFGNDPATGIAYA
                     RGWLYLGADDRILRWPWTPGQQVPEAAPVVVARGLPADGDHGAKPIVVRGGTVYVDFG
                     SATNSCQVHNRRPRSRGIDPCTELDIRAGIWAFPAGGSDQVPAAGHRFATGLRNAEAF
                     AFEPTTGTMWMAVHGRDQLGDNWGFSDSLNAELPAEEFGPVPEGADYGWPYCYYDELQ
                     GKKVTAPEYGGDGRRTDRCTGKALPVIGFPGHWAPMSLAFYDGTAFPPEYRGGAFLAF
                     HGSWNRAPLPQAGFRVVYIPFTGGKATGAYRTFATARPGVTFRPSGVAVGPDGALYIA
                     SDAAGKIWRVVATGPGTSRPRNAPGRQ"
     gene            3009..4685
                     /gene="ettA"
                     /locus_tag="VFU45_04180"
     CDS             3009..4685
                     /gene="ettA"
                     /locus_tag="VFU45_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEU5358293.1"
                     /translation="MAGEYIFTMRDLRKVVPPSREILKGLHLSFYPGAKIGVLGSNGA
                     GKATLLRIMAGIDTDFLGEAKPLAGTKIGYLPQEPLLDPAKDVRGNVEDGVRALRKIL
                     DEFNDVSARFAEPMTDQEMEKLLEKQGRLQDRIDHLGLWELDHKVDMAMDALRLPPGD
                     ADVSKLSGGEKRRVALCRLLLEQPDMLLLDEPTNHLDAESVAWLEHFLETFPGTVVAV
                     THDRYFLDNVAKWILELDRGAGIPWEGNYSSWLAQKQKRLAVEEKQASARQKTLEREL
                     EWVRMAPKARHAKSKARIQRYEELRAEGERQSEGTAEILIPVPERLGDEVVIAKDVAK
                     GFGDRLLFEKLNFSLPRGGIVGVIGPNGAGKTTLFRMITGAEKPDGGTLTVGQTVKVA
                     YVDQSRESLDGGKSVYEEISGGQDELLFGTRKVNARAYCAGFNFRGSDQQKKVKDLSG
                     GERNRVHLAKLLKSGGNLLLLDEPTNDLDVDTLRALEDALIGFAGCAVVISHDRWFLD
                     RIATHILAFEGDSEVVWYEGNYQEYAADHRKRKGGADDQPHRIKFKKLVH"
BASE COUNT          837 a         1700 c         1711 g          720 t
ORIGIN      
        1 tggccctggg gatcgtccgg gccggcgtgg cgcggcgggc tccgacccgg gcgccggtga
       61 ccgccagctg gtcgatgacc agcttctttt cgaagagcgc cgccgggtcg aggtcgaaca
      121 ccatccggtc ggcgtcgaac gtgttgcggg tggagtctcc ggcgtcggcg agttgcaggc
      181 cgtccagttc caccgagccg cggagcaggt ggatatggaa cgcggccagg tccacttccg
      241 cacccgtcat cttcgtcccg gcggctatca cggcgcgccg gccgagccag tcggcgaaga
      301 gcacccaccc gaggcagatg accagcagga agagcacgag ggggatgatc gccttccagc
      361 ggaagaggcg cgtggtcatc gcgtcactcc ggccggaaga ggcgatagta gttgtaaagc
      421 tgcgagccgg tcaccgcctt gtagatccgt gagcccatca ccttctgccc gatcgtggcc
      481 cggtagcgga ccacggccca ccggaagaag agcaccatcg gcacgaacag gaccagccac
      541 gacaccaggc tgccgagcgc cacggtgttg ttgaagttgg tgaacgggac gagcggcgtg
      601 ttggcgagcc gggtccagac gggcgtgagg cccggcgagt cgatcaggag tgcccggccc
      661 acccggtcga agacgggatc gagcgcgaag ccgagcggga ccgagatcac ccagccgagc
      721 atcccggccc cgaaggagac gttgagcagg aacagcagcg cgaagatcgc cacgttggtc
      781 aggctgaaca gcggcgtgag gccgagcgcg gcaccgagcg cgagcccgaa cgcgacctgg
      841 ttgggcgacc cgtcggagtg gagcgtcttg acgatcgact ggatgagctt gaggatggcg
      901 agcaccgacc ctccggagcc gggaatgcca gaagctagcg gggcgccaca cggggcggga
      961 gttgcgggaa cgccacagcc cggcagcgct ggtcttgcgc cgccgggctg ttgaaggagc
     1021 acagggcccc gactacgggt cgcccacggg cgtcacttcc ggggctttgt caccgccgtc
     1081 agcgccgagt cgagctggcg gtaggccagg gggtcgatct cgcggaacgg cgccgcaacg
     1141 taggcgatcc tgccgtcggg cccgaccacg tacagggtcc ggttgtcgac gtggtacttc
     1201 tcattccggg ccccgtagag ccggcccacc gcccccgccg tgtcgctcag gaagcggaag
     1261 gggaagttgc tttcccgggc ccagtcggcc agggtggtgt cggcgtccac gctgatggca
     1321 aggagcgtcg cccgcttgcc gttcccgaag agctgcgcgt actgatcacg gtacgcgtcc
     1381 atttggatgg ttcagccctt ggtccgcgcc tggaagaaga aggcgatcac gacggtctgg
     1441 tcgcggagat cgctcaggcg gatcggcttc gccgtgagcc catccttcgt ggcgcccggg
     1501 agcacgaagt ccggcgcgac cgttcccacc gcgaggggcg cgggtgccgc aggcggcgcc
     1561 tgctgcgccg ccacggtcgc cgcgccgagc agcgctgaga gcgcgaggcc atagtttcga
     1621 agtgcgttca cgtcattgcc tccccggtgc attgcgtggc cgggacgtcc ccggccctgt
     1681 ggccaccacc cgccagatct ttcccgccgc atctgatgct atatagagcg ctccatcggg
     1741 tccgaccgca acccctgagg ggcggaaggt caccccgggc cgggcggtgg caaaggtgcg
     1801 ataggccccc gtggccttcc cgcccgtgaa cgggatgtac accacgcgga acccggcctg
     1861 cggcagcggc gcccggttcc acgaaccgtg aaaggcgagg aacgcgccac cccggtactc
     1921 tggtgggaag gcggtgccgt cgtagaacgc cagcgacatc ggcgcccaat gtcccgggaa
     1981 tccgatcacg gggagcgcct ttccggtgca gcggtcggtg cgcctgccat cgccgccgta
     2041 ctcgggtgcc gtcaccttct tcccctgcag ctcatcgtag tagcagtacg gccacccgta
     2101 gtccgctccc tccggcaccg gcccgaactc ctccgccggc agctcggcgt tgagcgaatc
     2161 gctgaagccc caattgtccc ccagctggtc acgcccgtgc accgccatcc acatcgtccc
     2221 ggtcgtcggc tcgaaggcga acgcctccgc attccggagc cccgtggcga atcgatgtcc
     2281 cgcggcgggc acctgatcgg agcctccggc cgggaacgcc cagatgccgg cgcggatgtc
     2341 gagttcagtg cagggatcga tgccgcggga ccgggggcgc cggttgtgga cctggcaact
     2401 gttggtggcc gacccgaaat cgacgtagac ggtgccgccc cgtaccacga tcggcttggc
     2461 gccgtggtcg ccgtcggcgg gcagtccccg cgccaccacg accggggcgg cctccggcac
     2521 ctgctgcccg ggggtccatg gccagcggag gatccggtcg tccgcgccca ggtagagcca
     2581 gccacgcgca tatgcgatgc cggtggccgg gtcgttgccg aagtagttga cgacgtcggc
     2641 gtgtccatcg ccggtggtgt cacggaggag gaccgcccca ccccgctgcc gggcggccac
     2701 cacgtcgccg ttggggagga cggtcaggtt gcggggactg ccgagccctt cgccgaagag
     2761 cgtggcgcag aacccgggcg ggagcaccag gcccgcattg ccggggtcgc acggcggcgc
     2821 gaccggccgg gtcgccgcgg cgatcgtcgc ggcgccggcc aacccgatcg cggcgcggag
     2881 gacaggatgc atggacccct cgaggccagg gacgccggaa tatacgggcc acgacgcctg
     2941 acggttccac ggccggcggc gccggcgtcc ttcacgcgcg ccgcggcggc cattactttg
     3001 cccaccctat ggccggcgaa tacatcttca ccatgcgcga ccttcgcaag gtcgtgccac
     3061 cctcccgcga gatcctcaag gggctccatc tttccttcta tccgggcgcg aagatcggcg
     3121 tcctcggctc caacggcgcc gggaaggcga cgctcctgcg catcatggcc gggatcgaca
     3181 ccgatttcct gggcgaggcg aaaccgctcg ccggcacgaa gatcggatac ctgccgcagg
     3241 agccgctgct cgatcctgcc aaggacgtgc ggggcaacgt cgaggacggc gtgcgcgcgc
     3301 tccggaagat cctcgacgag ttcaacgacg tctcggcccg gttcgccgag ccgatgaccg
     3361 atcaggagat ggagaagctc ctcgagaagc aggggcgcct ccaggaccgg atcgaccacc
     3421 tcgggctgtg ggagctcgac cacaaggtcg acatggcgat ggacgcgctc cggctccccc
     3481 ccggcgatgc cgacgtctcg aagctctccg gcggtgagaa gcggcgggtg gcgctctgcc
     3541 ggctcctgct cgagcagccg gacatgctcc tgctcgacga gccgaccaac cacctcgacg
     3601 ccgagtcggt ggcctggctc gagcacttcc tcgagacgtt ccccgggacc gtcgtggccg
     3661 tgacgcacga ccggtacttc ctcgacaacg tggcgaagtg gatcctcgaa ctcgaccgcg
     3721 gggccggcat cccgtgggag gggaactact cctcctggct ggcccagaag cagaagcggc
     3781 tcgcggtgga ggagaagcag gcctccgcgc gccagaagac cctcgagcgc gagctcgagt
     3841 gggtgcggat ggcgcccaag gcccggcacg cgaagagcaa ggcgcgtatc cagcgctacg
     3901 aagagctgcg cgccgagggg gagcgtcagt cggaggggac ggcggagatc ctgatcccgg
     3961 tgcccgagcg cctgggcgac gaggtcgtca tcgccaagga cgtggcgaag ggcttcggcg
     4021 accggctgct cttcgagaag ctcaacttct cgctgccccg cggcgggatc gtcggcgtca
     4081 tcggccccaa cggcgccggc aagacgacgc tcttccgcat gatcaccggc gcagagaagc
     4141 ccgatggcgg cacgctcacc gtcgggcaga cggtgaaggt ggcgtacgtc gaccagagcc
     4201 gggaatcgct cgacggcggc aagagcgtct atgaggagat ctccggcggg caggacgagc
     4261 tcctcttcgg cacccgcaag gtcaacgccc gtgcctactg cgccggattc aacttccggg
     4321 gctccgacca gcagaagaag gtgaaggacc tctccggcgg cgagcgcaac cgggtccacc
     4381 tggccaagct cctgaagagc ggcggcaacc tcctcctgct cgacgagccc accaacgacc
     4441 tcgacgtgga caccctgcgg gcgctggagg atgcgctcat cggcttcgcg gggtgtgcgg
     4501 tcgtcatcag ccacgaccgc tggttcctcg accgcatcgc cacccacatc ctggccttcg
     4561 agggcgacag cgaggtggtc tggtacgagg ggaattacca ggagtatgcg gccgaccacc
     4621 ggaagcggaa gggcggcgcc gacgaccagc cgcaccggat caagttcaag aagctggtgc
     4681 attgaagaag taagaagtaa gaagtaagaa gtaagaagtg agaagggaga agggagaagg
     4741 gagagggggc tcggtgcgga gatccgcctc gagccccctt ctgacagttc ccttgtcctg
     4801 atccccaccc gttcccgctc acctcttacc tctcacctct tacctctcac ctcttacctc
     4861 tcacctctca cgtcttacgt ctcacctctc acgtcttacg tctcacctct cacgtcttac
     4921 gtctcacctc tcacgccccc cgccccccat ccgcgcccag agcgccag
//
LOCUS       DASUAP010000168         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_427306, whole genome shotgun sequence.
ACCESSION   DASUAP010000168 DASUAP010000000
VERSION     DASUAP010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427306"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VFU45_04185"
     CDS             <1..1066
                     /locus_tag="VFU45_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358294.1"
                     /translation="LYGAKGEYDSSLAYARRQIRVEPTARNAAVLAANLAGDGKLDSA
                     RTLMDSLLRVRPDAASNAQVAATRFLIAYARGQDDSAAAIATALDTSPQATAQVQALG
                     GLSRLAALRGELRRSVALDQQIDRILTQQGAPADPDGLSDAEADIRFRGRAAEGVHRL
                     DAIVAGKDWAALHPADRPYLRVATLYALAHRADRARQVLARFEQEDPGGFHAPIGQPD
                     LATIRGEIALAEGKPADALSQFRLENPLNGNGATRCDGCMAYDLARAFDAAGQADSAL
                     AEYQRYLSIPAGLRRDDEYRAAVQKRLGEIYDQRNDTKNAISHYAAFVDQWAHADAEL
                     QPVVNDVRRRLGELRGREGQ"
     gene            complement(1073..>3011)
                     /locus_tag="VFU45_04190"
     CDS             complement(1073..>3011)
                     /locus_tag="VFU45_04190"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU5358295.1"
                     /translation="TTAGGPAALRELTDSTGGRVTQVLKWTAAGEGRVTISGEVHGSP
                     EAFAAEAEPHHDALPVVRHAVGAVADRLDRAFYDRRWDWVLSVDPPAGAELVPVAGPD
                     SGISYRLTASGGEVALRFRPRFYQRHRGLAEFRPWEYRPWQQSVAGWSSWYAYFDRVT
                     EADIHRTADVMGEVLRPFGYRYLQIDDGYEQLPIGLPDHWLHPNAKFPGGLDALERYI
                     RSRGLEPAIWTNVSFADSAAAAAHPGWFVPGPDGRPAEGNWIGYVMDGSSAAAMDSLV
                     TPVYTALRRMGWSYFKLDALRHLRYEGYNSHAAYFGEKELDREQVFRSVVARVRQAIG
                     PDAYLLACWGIRPELIGLVDGVRVGDDGFGYGSFAEYNSFNNVVWRNDPDHIELHRAD
                     GYRAATVTSLTGSVLMLTDRPDVYRTDRVEVAKRTAPVLLTHPEQLYDVDPSRSSLLG
                     QAGTQLSGSGPRPFDADQRERVWLYQLDIARPFEQWTVLARTHDAPAAIPLAELGLLP
                     GVDYLAFDFWGRKYLGVLRDTLRGGAIDPRFEVQVTCLRRRVAHPQLLATGRHVTCGG
                     PDLKDVSWQDGALSGESELVAGDPYDIYLSEPAGYRFGRVEVTGAQMAGTRREGAMRV
                     VRLVSREGGAVRWVVRYGGGR"
BASE COUNT          449 a         1146 c         1031 g          385 t
ORIGIN      
        1 gctctatggt gccaaaggcg agtatgacag ctcgctcgcc tacgcccggc gccagatccg
       61 ggtcgagccg acggcccgca atgccgcggt cctggccgcg aacctggcgg gggacggcaa
      121 gctcgattcc gcccgcacgc tgatggactc gctgctgcgg gtccgccccg acgccgccag
      181 caatgcgcag gtagcggcca cgcgcttcct catcgcctac gcccgcggcc aggatgacag
      241 cgcggcggcg atcgcgacag ccttggacac ttctccgcag gccacggccc aggtgcaggc
      301 cctcggcggc ctcagccgcc tggccgcgct ccgcggcgag ctcaggcgct cggtggcgtt
      361 ggaccagcag atcgaccgga tcctcaccca gcagggcgcc cccgccgacc ccgacggcct
      421 gtcggacgcg gaggcagaca tccgattccg cgggcgcgcc gccgaaggcg tccaccggct
      481 cgatgccatc gtggccggca aggattgggc ggcactccac ccggccgacc gtccgtacct
      541 ccgggtcgcg acgctgtacg cgctggcaca tcgcgccgac cgggcacgcc aggtgctcgc
      601 gcgcttcgag caggaggacc cgggcgggtt ccacgccccg atcggccagc cggacctcgc
      661 cacgatccgc ggcgagatcg ccctcgccga ggggaagccg gcggacgccc tcagccagtt
      721 ccggctcgag aaccccctca acggcaatgg cgccacccgg tgcgacggct gcatggcata
      781 cgacctggcg cgcgccttcg acgccgccgg ccaggccgac tccgcactcg ccgagtacca
      841 gcggtacctc tcgatccccg ccgggctccg gcgtgacgac gaataccggg ccgccgtgca
      901 gaagcggctg ggcgagatct acgaccagcg gaatgacacc aagaacgcca tcagccacta
      961 tgccgcgttc gtcgaccagt gggcccacgc cgacgccgag cttcagccgg tcgtgaacga
     1021 cgtgcggcgg cggctcggcg aattgcgcgg acgcgaagga cagtagcccc ggctagcgcc
     1081 ccccgccgta ccgcaccacc caccgcaccg caccaccctc cctgctcaca agccggacca
     1141 cccgcatcgc tccctcgcgg cgcgtgccgg ccatctgcgc gcccgtgacc tcgacgcggc
     1201 cgaaccgata tcccgccggc tccgacaggt agatgtcgta gggatcgccc gccaccagct
     1261 cgctctcgcc gctcagcgcg ccatcctgcc acgacacgtc tttcaagtcg gggccgccgc
     1321 aggtcacgtg tcggccggtg gccaggagct gcggatgcgc cacccgccgc cggaggcagg
     1381 tcacctgcac ctcgaaccgc gggtcgatgg cgccgccacg cagggtatcg cgcagcacgc
     1441 ccaggtactt ccgcccccag aaatcgaagg cgaggtagtc gacgcccggc agcaggccga
     1501 gctcggccaa ggggatggcc gccggggcgt cgtgcgtgcg ggcgagcacc gtccactgct
     1561 cgaagggacg cgcgatgtcg agctggtaga gccatacccg ttcccgctgg tcggcgtcga
     1621 aggggcgcgg ccccgagccg ctcagctggg tccccgcctg gcccaggagc gatgagcggg
     1681 aggggtcgac gtcgtagagc tgttccggat gggtcaggag caccggcgcc gtccgcttgg
     1741 ccacttccac ccggtcggtg cggtacacgt cgggccggtc ggtcagcatc agcaccgacc
     1801 cggtgagcga ggtgaccgtg gcggcacgat atccgtcggc gcggtgcagc tcgatgtggt
     1861 cgggatcgtt ccgccagacg acgttgttga aggagttgta ctccgcgaac gacccgtagc
     1921 cgaagccgtc gtcgcccacg cggacgccgt ccaccagtcc gatcagctcg gggcggatcc
     1981 cccagcaggc cagcagatac gcgtcggggc cgatggcctg ccgcacccgc gccaccacgc
     2041 tccggaagac ctgctcgcga tcgagctcct tctccccgaa gtacgcggcg tggctgttgt
     2101 atccctcgta ccggaggtgc cggagcgcgt cgagcttgaa gtagctccac cccatccggc
     2161 ggagcgccgt gtacacgggc gtcacgaggg agtccatcgc agcggcggag ctcccgtcca
     2221 tcacgtagcc gatccagttc ccctccgccg ggcggccgtc ggggcccggc acgaaccagc
     2281 cggggtgcgc cgccgccgcc gcgctgtcgg caaagctcac gttggtccag atggcgggct
     2341 cgagcccgcg cgagcggatg tagcgctcca gggcgtcgag cccgccgggg aacttggcgt
     2401 tggggtggag ccaatggtcg gggagcccga tcgggagctg ctcgtagccg tcgtcgatct
     2461 gcaggtagcg gtagccgaat ggccggagca cctcccccat gacgtcggcg gtgcggtgga
     2521 tgtccgcttc cgtgacgcgg tcgaagtagg cgtaccacga cgaccacccc gccaccgact
     2581 gctgccacgg ccggtactcc cacggccgga actccgcgag cccacggtgg cgctggtaga
     2641 acctcggccg gaaccggagc gccacctccc cgccgctcgc cgtcagccga taggagatgc
     2701 cgctgtcggg cccggccacc ggcacaagct cggcaccggc cggcggatcc accgagagca
     2761 cccagtccca gcgccggtcg tagaaggcgc ggtcgagccg gtcggccacg gcgcccaccg
     2821 cgtggcgcac caccggcagg gcgtcgtggt gcggctccgc ctccgccgcg aacgcctcgg
     2881 gcgacccgtg gacctcgccg gaaatggtga cccgcccctc ccccgccgcc gtccacttga
     2941 ggacctgcgt cactcggccg ccggtgctgt cggtaagctc ccggagcgcc gccgggccac
     3001 ccgccgtggt g
//
LOCUS       DASUAP010000169         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_399877, whole genome shotgun sequence.
ACCESSION   DASUAP010000169 DASUAP010000000
VERSION     DASUAP010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399877"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFU45_04195"
     CDS             complement(<1..839)
                     /locus_tag="VFU45_04195"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU5358296.1"
                     /translation="MTNDYARIERAIRFLDAHPDASLEEVARDAGLSPFHFHRLFSRW
                     AGVSPKRFQQFQSVVEARRLLRDGRSVLDATYTAGLTSAGRLHDLMVTVDAVTPGEYK
                     ALGAGLVIRWGVHEGPFGPFLLGVTERGICSLRFVEPGTERSALAEARRHWPGARWVE
                     DVKGTAALARTVFGSLAPRSPSPTRHSPPAMRLFLKGTNFQLKVWEALLRIPEGSFAT
                     YQDVAELVCTADAVRATASAIGANPVLYLVPCHRVIRKTGAFGDYAGGETRKRALLGW
                     ERA"
     gene            970..1584
                     /gene="folE"
                     /locus_tag="VFU45_04200"
     CDS             970..1584
                     /gene="folE"
                     /locus_tag="VFU45_04200"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU5358297.1"
                     /translation="MAEIPMAMPDVLPFDGARCTRFEALVREMLELLGEDPDREGLRR
                     TPERVAKSLKFLTHGYALDAGDVISDALFPEESHGNMVLVRDIEFYSLCEHHLLPFHG
                     KVHVAYIPNGAIVGLSKLARVVDVFARRLQVQERLTDQVAAALERELKPRGVGVVVEA
                     VHMCMAMRGVEKQTSKTVTSAVRGSFRDDPRTRDEFLRLTMART"
     gene            complement(1815..2729)
                     /locus_tag="VFU45_04205"
     CDS             complement(1815..2729)
                     /locus_tag="VFU45_04205"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU5358298.1"
                     /translation="MLSLTLLGTGAACPTVDRNVASLAIHREGEMLLFDCGEGTQRQM
                     MRYGVGFSFDEIFFSHFHADHFLGLSGLLRTLGLLDRKAPLALYGPKGAEKWLGQAVA
                     LGMDRPKFPVEVQELKPGAVLKRAEYDIHAFPVDHRGEAFGYALIEHERLGRFHPDRA
                     KEFGVPEGPLWGKLHKGETVTLPDGRKVAPADLVGAPRPGRRVVYTGDTRPCRSVVEA
                     AKGADLLVHEATFGEEERDRAKETGHSTAREAAEVAERAGVKKLLLTHISARYTREAP
                     ELAAEARAVFPEAEVGRDGMSVEVPFET"
BASE COUNT          429 a          936 c         1002 g          417 t
ORIGIN      
        1 ctcgcccgct cccacccgag cagcgcccgc ttccgcgtct ccccaccagc gtagtcgccg
       61 aacgccccgg tcttccggat gacccggtgg cacggcacca ggtagagcac cgggttggcc
      121 ccgatggccg acgcggtggc gcggaccgca tcggcggtgc agaccaactc ggccacgtcc
      181 tggtacgtcg cgaagctccc ctccgggatc cggagcagcg cttcccagac cttgagctgg
      241 aagttggtgc ccttgaggaa gaggcgcatg gcgggtggcg agtggcgcgt gggtgacggg
      301 ctccgggggg cgagactccc gaatacggtc cgggccagag ccgcggtgcc cttcacgtcc
      361 tcgacccacc gggcgccggg ccagtgacgc cttgcctcgg cgagcgccga tcgctcggtc
      421 cccggctcga cgaaccggag cgagcagatc ccccgctcgg tgacgccgag gaggaatggg
      481 ccgaagggtc cctcgtgcac gccccaccgg atgacgaggc cggcgccgag agccttgtat
      541 tcgccgggcg tgacggcatc gaccgtgacc atcaggtcgt ggaggcgtcc tgcgctggtg
      601 aggccggcgg tgtaggtggc atcaaggacg ctccttccgt cgcggaggag gcggcgcgcc
      661 tcgacgacgc tctggaactg ctggaagcgc ttggggctca cgccggccca ccgggagaag
      721 agccggtgga agtgaaaggg ggagagtcca gcgtcccgtg ccacctcttc gagggaggcg
      781 tcgggatggg cgtcgaggaa ccggatggcg cgttcgatcc gcgcgtagtc gttcgtcatg
      841 ggcatacgtt agcgcggtgg accgccccgg gcgacccggt ccttgcggcg gcccctgctg
      901 cgcgggctgg acagaagctg gacggggaac cgatcggccg gtcggtgcgt actacgccac
      961 atccccccaa tggcggagat cccgatggcg atgcccgacg tgctgccgtt cgacggcgcg
     1021 cgctgcaccc gattcgaagc gctggtccgc gagatgctcg agctcctggg cgaagacccc
     1081 gaccgcgagg ggctccgccg cacccccgag cgggtggcca agagcctcaa gttcctgacc
     1141 cacggctatg ccctcgacgc cggcgacgtg atctccgacg ccctcttccc cgaggagagc
     1201 cacgggaaca tggtgctggt gcgcgacatc gagttctact cgctctgcga gcatcacctg
     1261 ctcccgttcc acggcaaggt gcacgtggcc tacatcccga acggcgccat cgtcgggctg
     1321 tcgaagctcg cccgggtggt cgacgtcttc gcccggcgcc tgcaggtgca ggagcgtctc
     1381 accgaccagg tcgcggccgc actcgagcgc gagctcaagc cgcggggcgt gggcgtcgtg
     1441 gtcgaggcgg tgcacatgtg catggcgatg cggggcgtgg aaaagcagac ctcgaagacc
     1501 gtcaccagcg ccgtgcgcgg gagcttccgt gacgacccgc ggacccggga cgagttcctg
     1561 cggttgacga tggcgagaac gtgagagatg agaaggaaga gggaaaaggg aagtgggaag
     1621 tgggaagtgg gaagtgccac ggaccagaag gcaagaggga cgagccagcg gggaggactt
     1681 gggagcagcc cgctgcatcg gggtcatcct gagggcgcgg agcgaccgaa ggatctctcg
     1741 gcctcccccg cccgctcttc cctgttccct attccccctt ttccctcttc cctcttccct
     1801 tttcctctct cgtctcacgt ctcgaacggc acctcaacgc tcatcccatc cctccccacc
     1861 tcggcctccg gaaacactgc ccgcgcttcc gcggcgagct ccggcgcctc gcgggtgtag
     1921 cgcgcgctga tgtgggtaag gagcagcttc ttgaccccgg cgcgctctgc cacctcggcg
     1981 gcctcccgcg cggtcgagtg gccggtctcc ttggcgcgat cccgctcttc ttcgccgaat
     2041 gtggcctcgt gcaccagcag gtccgcgccc ttggcggcct cgacgacgct ccggcagggc
     2101 cgcgtgtcgc cggtatagac gacccgtcgg ccggggcgcg gtgcgccgac caggtcggcg
     2161 ggggccacct tccggccatc gggcagcgtc acggtctcgc ccttgtggag cttgccccag
     2221 agcggccctt cagggacacc gaactccttg gcgcggtccg ggtggaagcg gccgagccgt
     2281 tcgtgctcga tgagggcgta accgaaggcc tcgccccggt ggtccacggg aaaggcgtgg
     2341 atgtcgtact cggcgcgctt gagcaccgcg cctggcttga gttcctgcac ctcgacaggg
     2401 aacttgggcc ggtccatccc gagtgccacc gcctgcccca gccacttctc cgcccctttg
     2461 gggccgtaga gcgcgagcgg agccttccgg tcgaggagcc cgagggtacg caggaggccc
     2521 gagaggccca ggaagtggtc ggcgtggaag tgggagaaga agatctcgtc gaacgagaac
     2581 ccgaccccgt aacgcatcat ctggcgctgg gtgccctcgc cgcagtcgaa cagcagcatc
     2641 tccccctcgc gatggatggc gagggaggcc acgttgcggt cgaccgtggg gcaggcggcg
     2701 ccggtgccga ggagggtgag ggagagcatg gggggaagat aagagcgggc gggtggagcg
     2761 ggcaaaaagc gggcaggtgg agcg
//
LOCUS       DASUAP010000170         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_672441, whole genome shotgun sequence.
ACCESSION   DASUAP010000170 DASUAP010000000
VERSION     DASUAP010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672441"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFU45_04210"
     CDS             <1..679
                     /locus_tag="VFU45_04210"
                     /inference="COORDINATES: protein motif:HMM:NF020249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HEU5358299.1"
                     /translation="DESSLNAHEAQLLTEQLQDLTGRAVPVRYEKLFTAQDGESLLRR
                     LPSHLAQEGVTALVFNFIDQLTHGRTEHTTLYEVARDAAAMRTLTRGWFERSALLEAL
                     KEADRRGVPVLLTSDHGSIHCHTPATVFAKRDATANLRYKFGDDLRVEDPDAAITVSD
                     LAAWGLPSRGPHERLLLATGDRFFVYPTKLREYQSRYRGAFLHGGVAPEEVILPIALL
                     TPRRARP"
     gene            676..2514
                     /locus_tag="VFU45_04215"
     CDS             676..2514
                     /locus_tag="VFU45_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358300.1"
                     /translation="MSTARRLFRELGAYRWGFLAAAGCMLVSSVLDGATVVMLIPLLK
                     HLFGSAGALSAGSSWLERFSDAALAPVLVGTTQDQAIARIVGLMVGILLLKNLAGYGA
                     NQIGVAVQEGVVRDLRIRLFRHMLTLDLGVFQRTRAGQLIQRIITDVDQIKSVINTSL
                     ARFFQNFSVILVTLAALTATSWRLTVLAVGAAPVLVLGLRGVLKRLRRHSRDRAEEWG
                     EITSYIAERLGAIKLIRGYGVAAQEAARFDAQASDYRRKVIRTNRYSSLTSPISEMFG
                     GLVLIMLLVGGTRPWVTGGGVPLSPATLITFLVLALRMMSPIKQISQFPADLAMALAS
                     ADRVFQLLDEPGGEADAPGARPASFSRELAFDHVSFSYDGERQVLRDISFLVPRGQAV
                     ALVGPSGAGKTTLVELLPRLHDPTSGEVRLDGVPLPRFTRASLRGLIGMVSQETVLLN
                     DTVRNNIAFGRRDATDLEVEAAARAANAHDFIADLPRGYATVLGERGTRLSGGQRQRI
                     AIARALLRDPPILLLDEATSALDTESERLVQEAIERLMQDRTVLVIAHRLATVRHADR
                     ILVIEDGRIVEQGTHDDLYRQGGMYRRLYDLQFRDEPSADPDPAAA"
     gene            2511..3488
                     /locus_tag="VFU45_04220"
     CDS             2511..3488
                     /locus_tag="VFU45_04220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5358301.1"
                     /translation="MTGAHPVFGGRKVLLISRFEGERHAHAPLRRRALERLGCGVAAV
                     DLQPGGWLARLTGGDSAARIARAVADHRPELVLALGPVLPDAAVVARFAAPGGTPWVL
                     WWGGDDGVEPPSAAWEAFTQVWVADSDLAARVPGARYLPHGCDPSVHRPMRSRDEFRA
                     NVVFVGEATPHRERLLAEVVEFGLAVWGPGWRRTRLRDYCRGDALAMDDYVRAYAGAS
                     VALNFHREGPDGRVPSAAGCNARTFELAAMGVAQVVDDRIDLASLFVDEEEVAVFREP
                     SELKPLVRELLQDPARAERLAAAARRRALAEHTYVHRLRRLLDAVEDAA"
     gene            complement(3507..>4273)
                     /locus_tag="VFU45_04225"
     CDS             complement(3507..>4273)
                     /locus_tag="VFU45_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5358302.1"
                     /translation="IGRLVADRTFRELERRLVVATVDLRSGAQVLWGLPGLDHVKVRE
                     AIFGSCALPGIFPPREVDGRLCVDGAVAESLPVRIAASECRGPVLAVHVGATSGLAES
                     ADPVGFAATYVRALEMVMHTMLVERLRGWESPPLLLVHPRVEHVGMFTFDHTRELIDE
                     GYRATMEALEQLPEGFPEHATGIYPRRTVEVSVEASRCVGCGACVMAAPAVFALEPDR
                     KARVLVPRQEWSPVDGGYVRGCPTFAIRAQLPEPPA"
BASE COUNT          541 a         1643 c         1504 g          585 t
ORIGIN      
        1 cgacgagtcg agcctcaacg cccacgaggc ccagctcctt accgagcagc tccaggacct
       61 caccggccgg gccgtgccgg tccgctacga gaagctcttc accgcccagg acggcgagtc
      121 gctgctccgg cggctgccgt cgcacctggc gcaggagggc gtcaccgcgc tggtcttcaa
      181 cttcatcgac cagctgacgc acggccgcac cgagcacacg acgctctacg aggtggcgcg
      241 cgacgccgcg gcgatgcgga ccctcacccg cggctggttc gagcgatcgg cgctcctcga
      301 ggcgctcaag gaggccgatc gccgcggcgt gccggtgctc ctcaccagcg accatggctc
      361 catccactgc cacaccccgg ccacggtgtt cgccaagcgg gacgccaccg ccaacctccg
      421 ctacaagttc ggcgacgacc tccgcgtcga ggacccggac gccgccatca cggtgagcga
      481 cctcgcggcc tggggcctcc cgtcgcgcgg accgcacgag cgcctgctgc tcgccaccgg
      541 cgaccggttc ttcgtctatc ccaccaagct ccgggagtac cagtcccgct accgcggcgc
      601 cttcctccac ggcggcgtcg cccccgaaga ggtgatcctg cccatcgccc tcctcacgcc
      661 ccgccgcgcc cggccgtgag cacggcccgg cggctcttcc gcgagctggg ggcgtaccgc
      721 tgggggttcc tcgcggcggc cggctgcatg ctggtgtcgt cggtcctcga cggcgccacg
      781 gtggtgatgc tcatcccgct cctcaagcac ctcttcggca gcgccggggc gctgagcgcc
      841 ggcagcagct ggctggagcg cttctccgac gcggcgctgg cgccggtgct ggtcggcacc
      901 acccaggacc aggcgatcgc gcggatcgtg gggctgatgg tgggcatcct gctgctgaag
      961 aacctggccg ggtacggcgc caaccagatc ggcgtggcgg tgcaggaggg cgtggtccgg
     1021 gacctccgca tccggctctt ccggcacatg ctgacgctcg acctcggcgt cttccagcgc
     1081 acccgcgccg ggcagctgat ccagcggatc atcaccgacg tcgaccagat caagagcgtc
     1141 atcaacacct cgctcgcgcg cttcttccag aacttcagcg tcatcctggt gacgctcgcg
     1201 gcgctcaccg ccacctcgtg gcggctcacc gtcctggcgg tcggcgcggc gccggtgctg
     1261 gtgctcggcc tccgcggcgt cctcaagcgg ctccggcgcc actcccgcga ccgcgccgag
     1321 gagtggggcg agatcaccag ctacatcgcc gagcggctcg gcgcgatcaa gctcatccgg
     1381 gggtacgggg tggccgcgca ggaggcggcg cgcttcgacg cgcaggcctc cgactatcgg
     1441 cggaaggtca tccgcaccaa ccggtactcc tcgctcacga gcccgatcag cgagatgttc
     1501 ggcgggctgg tgctcatcat gctcctggtc gggggcaccc ggccgtgggt caccggcggc
     1561 ggggtgccgc tctcgcccgc gacgctgatc accttcctcg tcctcgcgct ccggatgatg
     1621 tcgccgatca agcagatctc gcagttcccg gccgacctcg cgatggcgct ggcgagcgcc
     1681 gaccgcgtct tccagctcct cgacgagccc ggcggcgagg ccgacgcccc cggagcgcgg
     1741 ccggcgtcgt tctcccggga gctcgccttc gaccacgtgt ccttctccta cgacggcgag
     1801 cgccaggtcc tgcgcgacat ctcgttcctc gtgccccgcg gccaggcggt ggcgctcgtg
     1861 gggccgtccg gggcggggaa gacgacgttg gtcgagctcc ttccccggct ccacgacccc
     1921 acgtcgggcg aggtgcggct cgacggcgtg ccgctccccc ggttcacccg tgcttcgctc
     1981 cgcgggctca tcgggatggt cagccaggag acggtgctcc tcaacgacac ggtgcggaac
     2041 aacatcgcgt tcggccgccg cgatgccacc gacctggagg tggaggccgc cgcgcgcgcc
     2101 gccaacgcgc acgacttcat cgccgacctc ccgcggggct acgccacggt cctcggggag
     2161 cgtggcacgc gcctgtccgg cgggcagcgc cagcgcatcg ccatcgcgcg ggcactcctg
     2221 cgcgaccccc cgatcctcct cctcgacgag gccacgagcg cgctcgacac cgagtcggag
     2281 cggctggtcc aggaggccat cgagcggctc atgcaggacc ggacggtgct ggtcatcgcg
     2341 caccggctcg ccacggtccg ccacgccgac cggatcctgg tgatcgagga cgggcgcatc
     2401 gtggagcagg gcacgcacga cgacctctac cgccagggcg ggatgtaccg ccggctctac
     2461 gacctgcagt tccgcgacga gccgagcgcc gatcccgacc ccgccgcggc atgacgggcg
     2521 cccatccggt cttcggcggg cgcaaggtcc tgctcatctc gcggttcgaa ggcgaacgcc
     2581 acgcgcacgc cccgctccgg cgccgggccc tggagcgcct cggctgcggc gtcgcggccg
     2641 tcgacctgca gccgggcggg tggctcgcgc gcctgaccgg cggcgactct gcggcccgga
     2701 tcgcccgggc ggtggccgac caccgccccg agctggtcct cgcgctgggc ccggtgctcc
     2761 cggacgccgc ggtggtggcc cgcttcgccg cgccgggcgg cacgccgtgg gtgctctggt
     2821 ggggcggcga cgacggtgtc gagccgccgt cggccgcgtg ggaggccttc acccaggtct
     2881 gggtcgccga ttccgacctc gccgcgcggg tgccgggcgc gcgctacctc ccccacgggt
     2941 gcgacccgtc ggtgcaccgg ccgatgcgct cgcgggacga gttccgtgcc aacgtggtct
     3001 tcgtcggcga ggcgacgccc catcgggagc gcctgctggc ggaggtggtg gagttcgggc
     3061 tcgcggtctg ggggccgggc tggcgccgca cccggctgcg ggattactgc cgcggcgatg
     3121 cgctcgcgat ggacgattac gtgcgcgcgt atgccggcgc atcggtggcc ctcaacttcc
     3181 accgcgaggg gcccgatggc cgggtgccgt cggccgccgg atgcaacgcg cgcaccttcg
     3241 agctcgccgc catgggcgtg gcccaggtgg tggacgatcg catcgacctg gcgtcgctgt
     3301 tcgtcgacga ggaggaggtg gcggtcttcc gggagccgtc ggagctcaag ccgctggtgc
     3361 gcgagctgct gcaggatccg gcgcgggccg agcgactcgc cgccgcggcg cggcggcgcg
     3421 cactcgcgga gcacacgtac gtgcatcggc tgcggcggct gctcgatgcg gtcgaggacg
     3481 cggcctgacc gccgggatcc ggggcgctac gccgggggct ccggcagctg ggccctgatc
     3541 gcgaaggtgg ggcagccccg cacgtacccg ccgtcgaccg gcgaccattc ctggcgcggc
     3601 accagcacgc gggccttgcg gtccggctcg agcgcgaaca ccgccggcgc cgccatcacg
     3661 catgccccgc agccgacgca ccgcgacgcc tcgacggaga cctccaccgt gcgccgcggg
     3721 tagatccccg tcgcgtgctc cgggaatccc tccgggagct gctccagcgc ctccatcgtg
     3781 gcgcggtacc cctcgtcgat gagctcccgc gtatggtcga aggtgaacat tccgacgtgc
     3841 tccacccgcg gatgcaccag gagcagcggc ggggattccc agccgcgcag ccgctccacc
     3901 agcatcgtgt gcatgaccat ctcgagggcg cggacgtagg tcgccgcgaa ccccacgggg
     3961 tcggccgact ccgcgaggcc gctggtggcg ccgacatgca ccgccagcac cggtccccgg
     4021 cactcgctcg cggcgatgcg caccggcagg ctctcggcca ccgcgccgtc gacgcagagc
     4081 cggccgtcca cctcccgcgg cgggaagatg cccgggaggg cgcacgagcc gaagatcgcc
     4141 tcgcgcacct tgacgtggtc gagccccggc aggccccaga gcacctgggc cccgctgcgg
     4201 aggtcgacgg tcgccactac cagccgccgc tcgagctccc ggaaggtccg gtccgccacc
     4261 agccggccga tca
//
LOCUS       DASUAP010000171        10514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1231530, whole genome shotgun sequence.
ACCESSION   DASUAP010000171 DASUAP010000000
VERSION     DASUAP010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10514
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231530"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VFU45_04230"
     CDS             complement(<1..897)
                     /locus_tag="VFU45_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358303.1"
                     /translation="MSGRVADGGTPLMTQYREIKARHPNAILFFRMGDFYEMFFDDAE
                     LGAKTLGITLTSRGDGVPLAGVPVKAAADYLRQLLAAGLRVAICEQVEDPKAAKGIVR
                     REVVETITPGAVLDETFLAGTKNNFLVAAWVDEAHGRMGGLAAVDLSTGEFLLETVPG
                     ERLAESVSRLGPAEVVLPAESTLALDDGVMRTAREAWEFDPALAQDDLARRFQLASLD
                     GLGLGAEDAPAVGAAGALLRYLAELQPGGLPHLMRPVARRGEGHLWLDEMTRRNLELV
                     EPLRAGTRGATLIEVIDATVTPM"
     gene            complement(894..1799)
                     /locus_tag="VFU45_04235"
     CDS             complement(894..1799)
                     /locus_tag="VFU45_04235"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU5358304.1"
                     /translation="MTHLRWALACAAFLGVGASAPLFAQKDPRLLAAVAQAQQGQGDS
                     ARAAVAAILNATPATDPLYAEALYTQGRIAASTGDMQKAMLRVAVEFADSPWADNALL
                     QLAELSYANGDLAGTERNLERLRSDYPTTDVMAPASLWAARAYFAQGNTAAGCGWLKS
                     GIPAAGTNIELLNQLQFLNGRCQVADTTAQRRDTTVAPTPAPAPVPAPDTAKKPAPAP
                     APVTPAPPPTTWSVQVAALKAESQATTVMQMLARDGFPPHLVKDADGTFKVRAGKFAT
                     KAEATAYARKMRRKYGQVFVVEERP"
     gene            complement(1796..2878)
                     /gene="holA"
                     /locus_tag="VFU45_04240"
     CDS             complement(1796..2878)
                     /gene="holA"
                     /locus_tag="VFU45_04240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU5358305.1"
                     /translation="MPTLSVAALRKELKAGKLRPVYYFHGPEETLKEEAAQALLDAAL
                     DPSLRDFNFDQRSAQSLDAESLHALVNTLPMMAERRVVILRDVEALKKKGNARTALLK
                     YLDDPADGTMLILLQSAARKDERDDKADADLAAKAPSTVFDELDTDEAMAWVERTAKK
                     LGVQLAPDAVKHLVAAVGPELALLRLELQKFSALESPGPIDAKLVGALVGVQFGETIY
                     DWRDAILEGDLPRALRLSAPLLDQTGVSAVGMVTLLGSSLIVLGVARAHHDRKLAGFA
                     LNKAVFDTLMRARPPKIGDWKGFVNDVVRWAPAWPQGRLRRALEATLAADIALKGTRL
                     ATEQAVITDLILQLAPPSTGGRREVA"
     gene            complement(3138..3677)
                     /locus_tag="VFU45_04245"
     CDS             complement(3138..3677)
                     /locus_tag="VFU45_04245"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5358306.1"
                     /translation="MPLLSSDTLYTGRIFRLDRDTVRFPDGSVAPFDIIRHPGAAAVV
                     PFLDDPGDPDPRIVLLRQFRHAAGGEIWEIPAGRLEAGEDPATCAKRELEEETGYTAE
                     RVDHLLSLYTTPGFTDELIHLYSATGLRPGMAHREVDEFMELHELRWSEVGEMMTRGE
                     IQDAKTLVTLMWVQCFRRA"
     gene            complement(3899..5395)
                     /locus_tag="VFU45_04250"
     CDS             complement(3899..5395)
                     /locus_tag="VFU45_04250"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5358307.1"
                     /translation="MKSAISYQLSAISQQLTAFGRRSFGGLVGALLLTAFPATRLAAQ
                     VTAPPTLGAPRPFRSPRVETTRLPNGLTIDLVEMHKLPLVNVSLLVDGGGRLDGDLPG
                     LASFTADMLDEGAGTRDAIGIAAEAAYLGADLSTGSDWDRTTVSLQVPVRTLPAALDL
                     MADVALRPDFHTADIDKHRGLRMADILQRRDVPGAVATLVSNALIFPAGHPYHASPDG
                     DSTSTARLDSATVRRFYERISDPRRATIIVTGDITLARARALLARRFGAWKAHRAAAA
                     PKAPVPPPVERPTTLFLVDKPGAAQSVIIIGSPGVARTSSDYYALEVMNTILGGSFSS
                     RLNQDLRETKGYTYGAGSRFQFRPLPGPFTASAAVRTNVTDSSLADFFVQLRAIRDSL
                     VQPVELTRATQYIALGLPGDFETTGQVARQLSGLLLFGLPFTYYNGFVAHLEQVTRQD
                     VQRVARTYIDPDHLTVVVVGDLARIRAPIEALHLGPIEVLDAEGRAAK"
     gene            complement(5392..6795)
                     /locus_tag="VFU45_04255"
     CDS             complement(5392..6795)
                     /locus_tag="VFU45_04255"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5358308.1"
                     /translation="MTRIIPRSLAALVVASALASAASAQARPPARGAASRALTIPYSI
                     DTLPNGLTLIIHEDHTVPVVAVNTWYHVGSGDEEAHRTGFAHLFEHLMFMGSEHAPYP
                     MFDRLLEAAGADNNGTTNEDRTAYYESGPANALPLMLWLDADRMGWLLPTMDSAKVDL
                     QRDVVKNERRQRVENQPYGKAWDVIPSLLFPAGHPYSWPVIGSMADLSAASLEDVKDF
                     FRKFYAPNNATLVVSGDVQTPEVRAEVRKYFGAIPRGPAITRTPTPTFTLARDTAVVL
                     TDRVQLPRLYYMWHSTRAYGPDDAALNVLAYILAGAKNGRLTEPLVYTQQLAADVNAF
                     QEGRLRDGEFGIIATARPEHRLPELQAVIDSQVARLAADGPTERELEQAKNSFEASTL
                     EGLEYGSAVADQLNSYYYFTGTPDYLAKDIARTRAVTAADVERVARTYLQAPRVIVSV
                     VPEGKPELAASAAEVRP"
     gene            complement(6924..7526)
                     /locus_tag="VFU45_04260"
     CDS             complement(6924..7526)
                     /locus_tag="VFU45_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU5358309.1"
                     /translation="MSSFLRRRWRSVLGAGVTLAVLVYGASFVIVLTESREDQARPAD
                     AIVVLGAAQYNGRPSPVLKSRLDHGLELWRDRIAARIVVTGGIGDGDRMSEATVGQRY
                     LLSRGVPDTEVVVLARGRSTDETMAAVAGWLNDKGLSSVVLVSDPFHMARLRLEARGH
                     RVKAYTSPTRTSPISESPRDERRYLAAEAAKLPVVWVRSW"
     gene            complement(7528..8919)
                     /locus_tag="VFU45_04265"
     CDS             complement(7528..8919)
                     /locus_tag="VFU45_04265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5358310.1"
                     /translation="MSSVQLTFHGASSGHVTGSMYLLEAAQARFLLDAGLFQGTRADA
                     RALNTKLPFDARNINAIVLSHAHIDHSGRLPLLVRQGFHAPIYATPATRDLCAAMLPD
                     SAHIQEKDAEFLKRHGREVAEPLYNLADAIAVQELMIGLPYRRPFYLRKNLSFEFADA
                     GHILGSGSVDLRFAEGAGHRLVFSGDIGRWGLPIIRNPAPPSGPVDTLIIESTYADRE
                     HESVERTESLLGEIVARVAARGGKVLIPSFAVGRTQEIVFALHRLWGGKQIPEVPIFV
                     DSPLALDATQVFRMHPELFDKKERLVEEGSSLFDFPLVRYVRDVKESIALNSRPGPAV
                     IIAASGMAESGRITHHLVNQAGDHRNLILFVGFQAEHTLGRRIQEGQKEVRILGTNVP
                     VRAEVATLGGYSAHADKHELRKWVRLLGGPVKRAFVVHGEDHARKAMAEILKSEGVDE
                     VVLPELGQSFKLQ"
     gene            complement(9051..9695)
                     /gene="upp"
                     /locus_tag="VFU45_04270"
     CDS             complement(9051..9695)
                     /gene="upp"
                     /locus_tag="VFU45_04270"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU5358311.1"
                     /translation="MPHPRHPNLTVLDHPLIRHKVTLLRDQRTGTRDFRQLVAEIAML
                     MAFEVTQDLPGRDIPVETPLERTTGFEVDSKRVVLVPILRAGLGMVEGISQLMPTARV
                     GHVGLYRDHDSLQPISYYFKLPPAEGERELLVLDPMLATGGSAAAAVGKLKETGPARI
                     KFLCLVAAPEGVIAMQKAHPEVPVFAAALDRQLNDQGYILPGLGDAGDRLFGTK"
     gene            9887..9959
                     /locus_tag="VFU45_04275"
     tRNA            9887..9959
                     /locus_tag="VFU45_04275"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9920..9922,aa:Phe,seq:gaa)
     gene            10319..>10514
                     /locus_tag="VFU45_04280"
     CDS             10319..>10514
                     /locus_tag="VFU45_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358312.1"
                     /translation="MPAPAAVVRVSPACDSLAQAIRSGAAHPTISQPYARELSLPPRG
                     APADLRENELTVSWQVAASGQ"
BASE COUNT         1456 a         3456 c         3943 g         1659 t
ORIGIN      
        1 catcggcgtc acggtggcgt cgatgacctc gatgagcgtg gcgccccggg tgccggcgcg
       61 gagcggctcc accagctcga ggttgcggcg ggtcatctcg tcgagccaga ggtggccctc
      121 gccgcggcgc gccaccgggc gcatcaggtg cgggaggccg ccgggctgga gctcggccag
      181 gtaccggagg agcgcccccg ccgcgcccac ggccggcgcg tcctcggcgc cgaggccgag
      241 gccgtcgagc gaggcgagct ggaagcggcg cgcgaggtcg tcctgggcga gggcggggtc
      301 gaactcccac gcctcgcgcg cggtgcgcat cacgccgtca tcgagcgcga gcgtcgactc
      361 cgccggcagg accacctcgg cggggccgag ccgcgagacc gactccgcga gccgctcgcc
      421 cggcaccgtc tccaggagga actcgccggt ggagaggtcg acggccgcca ggccgcccat
      481 ccgcccgtgc gcctcatcca cccacgcggc gaccaggaag ttgttcttcg tcccggcgag
      541 aaaggtctcg tcgagcaccg cgccgggggt gatcgtctcc accacttcgc ggcggacgat
      601 ccccttggcg gccttcgggt cttcgacctg ttcgcagatg gcgacgcgga ggccggcggc
      661 gaggagctgg cggaggtagt cggcggcggc cttgaccggc acgccggcga gcggcacgcc
      721 gtcgccgcgg gacgtgaggg tgatgccgag cgtcttcgcg ccgagttcgg cgtcgtcgaa
      781 gaacatctcg tagaagtcgc ccatgcggaa gaagaggatc gcgttggggt gccgcgcctt
      841 gatctcccgg tactgcgtca tcaggggcgt gccgccatcg gccacgcggc cgctcaaggg
      901 cgctcctcga cgacgaacac ctggccgtac ttgcgccgca tcttccgggc gtaggccgtc
      961 gcctccgcct tggtggcgaa cttgccggcc cgcaccttga acgtgccgtc ggcgtccttg
     1021 acgaggtggg gcgggaagcc gtcgcgggcg agcatctgca tcaccgtggt ggcctgcgac
     1081 tccgccttga gggcggcgac ctggacggac caggtggtgg gtggaggggc gggagtgacg
     1141 ggcgcgggcg ccggcgccgg cttcttggcg gtgtcgggcg ccggcactgg cgccggtgcc
     1201 ggcgtggggg caaccgtcgt gtcgcgccgc tgggcggtgg tgtcggcgac ctggcagcgg
     1261 ccgttcagga actggagctg gttcaggagc tcgatgttgg tgcccgcggc agggatcccg
     1321 ctcttgagcc agccgcaccc ggcggcggtg ttcccctgcg caaagtaggc ccgcgccgcc
     1381 cagagcgagg ccggtgccat cacgtcggtg gtcgggtagt cgctccgcag gcgctcgagg
     1441 ttccgctcgg tgccggccag gtcgccgttg gcgtagctca gttcggcgag ctggaggagc
     1501 gcgttgtcgg cccacggcga gtcggcgaac tcgaccgcta cgcgcagcat cgccttctgc
     1561 atgtcgccgg tgctggccgc gatccgtccc tgggtataga gcgcctcggc gtagagcggg
     1621 tcggtggccg gcgtcgcgtt gaggatcgcg gcgacggccg cgcgggccga gtcgccctgg
     1681 ccctgctgcg cctgggcgac ggcggccagg aggcggggat ccttctgggc gaacagcggc
     1741 gcggacgccc cgacaccaag gaacgcggcg catgcgagcg cccaacggag atgcgtcatg
     1801 cgacctcccg gcgtccgccg gtggacgggg gcgcgagctg gaggatgaga tcggtgatga
     1861 cggcctgctc ggtggcgagg cgcgtgccct tgagggcgat gtcggcggcg agcgtggcct
     1921 cgagggcgcg gcggagccgc ccctgcggcc aggccggcgc ccagcggacc acgtcgttca
     1981 cgaagccctt ccagtcgccg atcttggggg gccgggcgcg catcagggtg tcgaagaccg
     2041 ccttgttgag cgcaaagccc gcgagcttcc ggtcgtggtg tgcccgcgcc acgccgagca
     2101 cgatgagcga actgccgagg agcgtcacca tccccaccgc gctcacgccg gtctggtcga
     2161 ggaggggggc gctcaagcgg agcgcgcggg ggaggtcgcc ctcgaggatg gcatcacgcc
     2221 agtcgtagat ggtctcgccg aactggacgc ccaccagcgc gcccaccagc ttcgcgtcga
     2281 tcggcccggg cgactccagc gcggagaact tctgcaactc gagccgcagg agcgcgagct
     2341 ccggccccac cgcggccacc aggtgcttga cggcgtcggg ggcgagctgc acgccgagct
     2401 tcttcgccgt gcgctccacc caggccatcg cctcgtcggt gtcgagttcg tcgaacaccg
     2461 tcgacggcgc cttggcggcg aggtcggcgt cggccttgtc gtcgcgctcg tccttgcggg
     2521 cggccgactg cagcaggatg agcatcgtgc cgtcagcggg gtcgtcgagg tacttgagga
     2581 gcgcggtgcg ggcgttgccc ttcttcttca gggcctcgac gtcgcggagg atgacgaccc
     2641 ggcgctcggc catcatcggc agggtgttga ccagggcgtg gagcgattcc gcgtcgagcg
     2701 actgggcgga gcgctggtcg aagttgaagt cgcggaggga ggggtcgagc gccgcgtcga
     2761 ggagcgcctg cgccgcctcc tccttgaggg tctcctcggg gccgtggaag tagtagacgg
     2821 ggcgcagctt ccccgccttc aactccttcc ggagcgcggc cacggagagg gtcggcattg
     2881 ggcggaaaag taccggtttt ggcggggggt gcaaagagca agaaagacat gggaccaccc
     2941 ttcgctgcgc gcaggatctc aagccgggcg ttgcccgagg cgcgatcacg ccacgctccg
     3001 agcccgttca gcatcgggtc gagatccttc ggtcgctccg ctccctcagg atgacaccgg
     3061 tggtgggcct gctcccgctc cctgagccct aagccctaag ccctaagctc taagctctaa
     3121 gctctaagcc ctaagctcta agctctccgg aagcactgca cccacatcag cgtgaccagg
     3181 gtcttggcgt cctggatctc gccgcgggtc atcatctcgc ccacctcgga ccagcggagc
     3241 tcgtggagtt ccatgaactc gtcgacctcg cggtgcgcca tcccggggcg gagcccggtg
     3301 gcagagtaaa ggtggatgag ctcatcggtg aagcccggcg tggtgtacag gctcagcagg
     3361 tgatcgaccc gttcggcggt gtagccggtc tcctcttcca gctcgcgctt ggcgcaggtg
     3421 gcggggtctt cgcccgcttc caggcgtccg gcagggatct cccagatctc gccccccgcc
     3481 gcgtggcgga actggcggag gaggacgatg cgcgggtcgg gatcgccggg gtcgtcgagg
     3541 aaggggacca ccgcggcggc gccggggtgc cggatgatgt cgaagggcgc caccgagccg
     3601 tccgggaacc gcaccgtgtc ccggtcgagg cggaagatcc tgccggtgta gagggtgtcg
     3661 gaggagagca ggggcatttt ggggggaccg gtgaacggtg aagggtgaac ggtgaacggg
     3721 tccgtcgtcc ctgacgtcgg tgccggcctt cgaacggaac ataggcgatc ggcgacgcat
     3781 ccgcaccggc ggaactcgcg ctgatccctg cctccatcgg tgcatcgagc ggctggaatg
     3841 cgaccagggg cccgcgctga cgggcccccg ttcactgttg accgttcacc gttcacctct
     3901 acttcgccgc cctcccctcg gcgtcgagca cctcgatcgg ccccaggtgg agcgcctcga
     3961 tgggggcgcg gatcctggcc aggtcgccca cgacgaccac ggtcaggtgg tcggggtcga
     4021 tgtaggtgcg cgccacgcgc tggacgtcct gccgggtgac ctgctccagg tgcgccacga
     4081 acccgttgta gtaggtgaac ggcagtccga agagaagcag tccgctcagc tggcgcgcca
     4141 cctggccggt ggtctcgaaa tctcccggga gtccgagagc gatgtactgg gtggcgcggg
     4201 tgagctcgac cggctggacc agggaatcgc ggatggcgcg gagctggacg aagaagtccg
     4261 cgagcgagct gtcggtgacg ttggtgcgga ccgcggcgct ggcggtgaag gggccgggga
     4321 ggggccggaa ctggaagcgc gagccggcgc cataggtgta gcccttggtc tcccgcaggt
     4381 cctggttcag gcgggaggag aacgacccgc cgaggatcgt gttcatcacc tcgagggcgt
     4441 agtaatcgga tgacgtccgc gcgacgccgg gcgagccgat gatgatgacc gattgcgcgg
     4501 cgccgggctt gtcgaccagg aagagcgtcg tcggccgctc gaccggcggc ggcaccggtg
     4561 ccttcggggc ggcggcggct cggtgcgcct tccacgcgcc gaaccgccgg gccaggagcg
     4621 cccgggcccg cgcgagggtg atgtcgccgg tgacgatgat cgtggcccgg cgggggtcgc
     4681 tgatgcgctc gtagaaccgg cgcaccgtgg ccgagtcgag ccgggcggtg gaggtggaat
     4741 cgccgtcggg gctcgcgtga tacggatgtc ccgccgggaa gatgagcgcg ttgctcacca
     4801 gcgtcgcgac ggctcccggc acgtcccgcc gctggaggat gtccgccatc cggaggccgc
     4861 ggtgcttgtc gatgtcggcg gtgtggaagt ccggccggag cgccacgtcg gccatcaggt
     4921 cgagcgcggc ggggagggtg cgcaccggca cctggagcga caccgtggtg cgatcccagt
     4981 cggagccggt gctgaggtcg gcgccgaggt acgccgcctc ggccgcgatg ccgatcgcgt
     5041 cacgggtgcc ggcgccttcg tccagcatgt cggcggtgaa gctcgcgagg ccggggaggt
     5101 cgccgtcgag ccgcccgccc ccgtcgacca ggagcgacac gttgacgagc ggcagcttgt
     5161 gcatctcgac caggtcgatc gtgagcccgt tggggaggcg ggtcgtctcg accctgggac
     5221 tgcggaacgg cctcggcgcg ccgagcgtcg gcggggcggt gacctgggcg gccaggcggg
     5281 tagccgggaa ggcggtaagc agcagtgcac cgaccaggcc cccgaatgac cgacgaccga
     5341 acgctgtcag ctgttggctg atagctgata gctgatagct gatagcgctc ttcacggcct
     5401 cacctccgcc gcgctggcgg caagctcggg cttcccttca gggaccacgc tcacgatgac
     5461 cctcggcgcc tgcaggtagg tgcgggccac gcgctccacg tccgccgcgg tgaccgcgcg
     5521 ggtgcgggcg atgtccttcg cgaggtagtc gggcgtgccg gtgaagtagt agtacgagtt
     5581 cagctggtcg gcgacggcgc tgccgtactc cagcccctcg agcgtcgacg cctcgaagct
     5641 gttcttggcc tgctcgagct cgcgctcggt cggcccgtcg gcggcgaggc gcgccacctg
     5701 ggagtcgatg accgcctgca gctccgggag ccggtgttcc gggcgcgccg tggcgatgat
     5761 gccgaattcg ccgtcgcgga ggcgcccctc ctggaaggcg ttgacgtcgg cggcgagctg
     5821 ctgggtgtag accagcggtt cggtgaggcg cccgttcttg gcgccggcca ggatgtaggc
     5881 caggacgttg agcgccgcgt cgtcgggccc gtaggcgcgg gtggagtgcc acatatagta
     5941 gagccgcgga agctggaccc ggtcggtgag gaccaccgcg gtatcgcggg ccagggtgaa
     6001 cgtcggcgtc ggcgtccggg tgatggcggg gccccgcggg atggccccga agtacttccg
     6061 cacctcggcc cggacctccg gggtctgcac gtcgcccgaa acgacgaggg tggcgttgtt
     6121 cggcgcgtag aacttccgga agaagtcctt cacgtcttcg aggctcgcgg cggagagatc
     6181 ggccatcgag ccgatgaccg gccacgagta ggggtggccg gccgggaaga ggagcgaggg
     6241 gatcacgtcc cacgccttgc cgtagggctg gttctccacc cgctggcggc gctcgttctt
     6301 caccacgtcg cgctggaggt ccaccttggc gctgtccatc gtggggagga gccaccccat
     6361 ccggtcggcg tcgagccaga gcatcagcgg cagcgcgttg gccggcccgc tctcgtaata
     6421 ggcggtgcgg tcctcgttgg tggtgccgtt gttgtcggca ccggcggcct cgaggagccg
     6481 gtcgaacatc gggtagggcg cgtgctccga gcccatgaac atcaggtgct cgaagaggtg
     6541 cgcgaacccg gtgcgatgcg cctcctcgtc cccggagccg acgtggtacc aggtgttgac
     6601 ggccaccacg gggaccgtgt ggtcttcgtg gatgatgagc gttaggccgt tggggagtgt
     6661 gtcgatggag taggggatcg tgagcgccct ggacgcggcg ccacgggccg gcggccgcgc
     6721 ctgcgcgctg gcggcgctgg cgagtgccga ggcgacgacg agcgccgcca gggatcgggg
     6781 gatgattctg gtcatttcag gtctcattgg aagttgtcgt ccgtccgccg tgcgcctcgc
     6841 cccctgcccc ctcttcccct ttcccttttc cccctcaccg ctcaccgctc accgctcacg
     6901 tcttacgtct caccgctcac ctcttaccaa ctccgcaccc acaccaccgg cagcttcgcc
     6961 gcttcggccg ccaggtaccg gcgctcgtcc cgcgggctct cggagatggg actcgttcgc
     7021 gtgggcgagg tgtacgcctt gacccggtgg ccgcgggcct cgagccggag gcgggccatg
     7081 tggaacgggt cactcaccag cactaccgaa ctcagcccct tgtcgttgag ccatcccgcc
     7141 actgccgcca tcgtctcgtc ggtcgagcgg ccccgggcca gcaccactac ctcggtgtcc
     7201 ggcaccccgc gggagaggag gtagcgctgg cccaccgtgg cctcgctcat ccggtcgccg
     7261 tcgccgatgc cgccggtcac cacgatccgc gcggcgatcc ggtcgcgcca gagctcgagg
     7321 ccgtggtcga gccgcgactt gagcactggc gaggggcggc cgttgtactg cgccgcgccc
     7381 aggaccacga tggcgtcggc ggggcgggcc tggtcctccc ggctctcggt gaggacgatg
     7441 acgaagcttg ccccgtacac cagcacggcg agggtgaccc cggcgccgag caccgacctc
     7501 caccggcggc gcaggaagga gctcacgcta ctgcagcttg aagctctggc cgagctccgg
     7561 cagcaccacc tcgtccacgc cctcggactt gaggatctcg gccatcgcct tgcgggcgtg
     7621 gtcctcgccg tgcaccacga aggcgcgctt gaccggtccc ccaaggagcc gcacccactt
     7681 ccggagctcg tgcttgtcgg cgtgcgccga gtagccgccg agggtggcca cctcggcccg
     7741 caccggcacg ttcgtgccca ggatccggac ctccttctgg ccctcctgga tccggcggcc
     7801 gagggtgtgc tccgcctgga acccgacgaa gaggatcagg ttccggtggt cgccggcctg
     7861 gttgaccagg tggtgggtga tgcgtcccga ctccgccatc cccgacgcgg cgatgatgac
     7921 cgccggtccc ggacggctgt tgagcgcgat cgattccttt acgtcgcgca cgtagcggac
     7981 cagcgggaag tcgaagaggc tgcttccctc ctcgacgagc cgctccttct tgtcgaagag
     8041 ctcggggtgc atccggaaga cctgcgtcgc gtcgagcgcg agggggctgt cgacgaagat
     8101 cggcacctcg gggatctgct tgccgcccca gaggcggtgg agggcgaaga cgatctcctg
     8161 cgtccggccg accgcgaacg acgggatcag caccttgccg ccccgggcgg ccacccgggc
     8221 cacgatctcg cccaggagcg actcggtgcg ctccaccgac tcgtgttctc gatccgcgta
     8281 ggtggactcg atgatcagcg tgtcgaccgg gccgctgggg ggcgccgggt tccggatgat
     8341 cgggagtccc cagcggccga tgtcgcccga gaagacgagc cggtgtccgg cgccctcggc
     8401 aaagcggagg tccaccgagc cggagccgag gatgtggccc gcgtcggcga actcgaagga
     8461 aaggttcttc cggaggtaga agggtcggcg gtacggcagg ccgatcatca gctcctggac
     8521 cgcgatggca tcggccaggt tgtagagcgg ctccgccacc tcgcggccgt ggcgcttgag
     8581 gaactcggcg tccttttcct ggatgtgggc cgagtcgggg agcatcgcgg cgcagaggtc
     8641 gcgggtggcg ggcgtggcgt agatcggcgc gtggaagccc tggcggacca ggagcgggag
     8701 ccggccgcta tggtcgatgt gggcgtggct caggacgatc gcgttgatgt tgcgggcgtc
     8761 gaaggggagc ttggtgttga gggcgcgcgc gtcggcgcgc gtcccctgga agaggcccgc
     8821 atcgaggagg aagcgtgcct gcgccgcctc gaggaggtac atcgagccgg tcacgtggcc
     8881 gctggaggcg ccgtggaagg tcagctggac ggaggacaat ggggtcgggg gtcgttggtc
     8941 attggtcggg ggtcgttggt cgtgagtcgt tggtcattgg tcgcgggtcg tgggtcacgg
     9001 gtcgcgggac acgggtcaca catcgcgacc tgcgactcgt gacccgcgac ctacttcgtc
     9061 ccaaacagcc ggtcccccgc gtcgccgagg ccggggagga tgtagccctg gtcgttgagc
     9121 tggcggtcga gcgcggcggc gaagaccggc acttccgggt gcgccttctg catcgcgatg
     9181 actccttcgg gggccgccac caggcagagg aacttgatcc gtgcggggcc cgtctccttg
     9241 agcttcccca ccgcggccgc cgccgagccg ccggtggcga gcatcgggtc gagcaccagg
     9301 agttcgcgtt cgccttcggc gggggggagc ttgaagtagt agctgatcgg ctggagggag
     9361 tcgtggtcgc ggtagagccc cacgtggccc acacgcgcgg tgggcatcaa ttgggagatc
     9421 ccttcgacca tgccgagccc ggcgcggagg atcggcacca ggacgacgcg cttgctgtcg
     9481 acctcgaagc cggtggtgcg ctcgagcggg gtctcgaccg ggatgtcacg acccgggagg
     9541 tcctgggtca cctcgaacgc catcagcatc gcgatctcgg ccacgagctg gcggaagtcg
     9601 cgggtgccgg tccgctggtc acgcaggagg gtgaccttgt ggcggatcag cgggtggtcg
     9661 aggacggtga ggttagggtg tcgggggtgg ggcatatcta atagtaggcg gtgaggcggc
     9721 ggggccgtaa ggcggcatgg cggccggggg gcatggcggt cgggaggtcc gtgatcgcat
     9781 cgaccgaccc taagtcgttg cccgatggcg cattgagccc tccgcttccg atgcctcacc
     9841 gccctaccgt cataccgccg taccgcctat aattcccgcc cttccgggct aggtagctca
     9901 gttggtagag cagcggactg aaaatccgcg tgtcgggggt tcgattccct ccctagccat
     9961 gtctgcggtg agcggtaagc ggtgaacggt gaacgggggc tccgatgagg atcggcgccc
    10021 cttctgcgtc acggggttcg gtctgaccac ggagtcatcc tgagcggagc gcctgccctg
    10081 agctggccga agggaaggat ctctcgggtc ggcgcatccc catcccgctg agcttctggc
    10141 cctcccatcg cacgcgattc atattgtgca gtgatgtggg ttcgtcgggt cacggacgac
    10201 cttcccgccg ttaggcaccg tcagcgcccc cgatcgagga tcgatgatgc aaagcgcatc
    10261 ccgttggctc cttctctgtg tggcagcgat cgctgttgcg tgcgcgcatc cggccccggt
    10321 gcccgctcct gccgccgtgg tccgggtcag ccccgcgtgt gactccctcg cgcaggccat
    10381 ccgatccggc gcggcacacc cgaccataag ccagccctac gcccgcgagc tctcgctgcc
    10441 gccccggggc gctccggcgg acttgcgcga gaacgagttg acggtgtcgt ggcaggtcgc
    10501 ggcctccggt cagg
//
LOCUS       DASUAP010000172         4730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_208881, whole genome shotgun sequence.
ACCESSION   DASUAP010000172 DASUAP010000000
VERSION     DASUAP010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208881"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..820)
                     /locus_tag="VFU45_04285"
     CDS             complement(101..820)
                     /locus_tag="VFU45_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358313.1"
                     /translation="MRYILGVLALLLCPAGLRAQLRDVTDQQPPPGPIRGQAGGWLEY
                     AAPTGDFGRFVHDGFGLTGWGGWVLDRGGRVTVGFELGIVNYGDRTRTVPLSPTIPGL
                     YADVTTTNNIVTLGTPVVRVDLARGSIRPYVSGSVGAAYFYTQTSARGTSSGGAFASS
                     TNFSDWTFNWSAGAGVAIQLSWGKHPLALDFGVRHRDNARVQYLNENSIQGTGSGTTV
                     TPIESEANLTVWGLGVVFGLR"
     gene            987..1289
                     /locus_tag="VFU45_04290"
     CDS             987..1289
                     /locus_tag="VFU45_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5358314.1"
                     /translation="MPTYEYACPAGHAFELFQKMSDKPRAKCPVCGRAAVRRISGGAG
                     LVFKGSGFYITDYGKDGKGPRKDPVDSAPAPKADVKPDTKAEPTPKPKAKAPKAGE"
     gene            1286..2926
                     /gene="argS"
                     /locus_tag="VFU45_04295"
     CDS             1286..2926
                     /gene="argS"
                     /locus_tag="VFU45_04295"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU5358315.1"
                     /translation="MSEQLRSELARIAAKLGGDGVPFVLERPREDAHGDWATNLALLL
                     AQAQKARPREVAERIVAELALPAGVVARTEIAGPGFINFWLGKAQLANDLATIIGAGA
                     RYGRHTFGAGRKVNIEFVSANPTGPLHVGHGRGAALGDGIAALLEWTGHAVTREFYIN
                     DAGVQIDKLARSLWARVQEAAGRKAEIPDGGYHGSYLSESAAKVLEREGKGFADLPEA
                     DGVARCRALALRMMREEQDEDLRQFGVVFQAMTSEQALYDGGKVEAGLKLLAARKLTF
                     EQDGALWLRTTDFGDDKDRVLRKSDGSLTYLVPDIAYHIDKHERGFDLAIDVWGADHH
                     GYIPRMRAVLLALGYPDGFFDVALVQLVKVVRGGEEVKMSKRSGEFVTLRDLFEEVGV
                     DAARYFFLMRRGDSHLIFDIDLAKSQTDENPVFYVQMAHARMSGIFRVAGREPASADP
                     AAVDVAALSAPQDQDLLKTLTVFPDVVAAAARAREPHRITAYLEDLARLAHNWYHHCR
                     VLGEPPAVEAARLALARAARTVLANGLTLLGLTAPDRM"
     gene            2929..3849
                     /locus_tag="VFU45_04300"
     CDS             2929..3849
                     /locus_tag="VFU45_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5358316.1"
                     /translation="MTLLVVGSVALDSIYTPFGETADALGGSAVFFSVAASLLTTVKV
                     VGVIGSDYPVAELERLAPRGIDWSGVERAEGESFRWKGKYSYDLQSRETLETRLGVFA
                     DFQPKLPPAFRGAEYLFLGNIDPELQLGVLSQIERPKLVACDTMNYWIQSKKDVLLQL
                     LKQVDILMVNDSEARELSGDWNIHRAGRWILAHGPSRVVIKQGEYGALLMEPGGTFAI
                     PGYPLEEVFDPTGAGDAFAGGFMGYLARSGDGTDAALRRAMVYGSVMGSFAVEQFGTR
                     GFDDVDDGDVENRVDGFRQLTAVHFPGRSA"
     gene            3846..>4730
                     /gene="purM"
                     /locus_tag="VFU45_04305"
     CDS             3846..>4730
                     /gene="purM"
                     /locus_tag="VFU45_04305"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU5358317.1"
                     /translation="MTGRQYAAAGVDLDTAEDAKARIGRLVAGTRTPLSLGEVGAFGG
                     MVRVPPGYRSPVLVMSTDGVGTKVLVALEAGRLDTVGEDLVNHSVNDILVHGARPVAF
                     QDYVAGSALTAELIAAVVEGVARGCRNHQMALTGGETAMMPGLYQPGHFDLAGTILGV
                     VEEDQAIHGHRIVPGDTLLGYASSGLHTNGYTLARRILFDVMKLQLSAPLADTGRTVV
                     DHLLAVHVSYAGAVMPVISRVHGIAHITGGGIPGNLVRVLPPACEAVVDAGSWTLPPL
                     FQVLQRAGQVSTEEMRDVF"
BASE COUNT          703 a         1628 c         1708 g          691 t
ORIGIN      
        1 ggtgagaggt aagaggtgag aggtaagagg tgagaggtaa gcgggctgcc gggatgacga
       61 gcgacggcgg cccgcagtcg ttccggcggc cgcgcggcgc tcatcgcagc ccgaagacca
      121 cgcccagccc ccacaccgtc aggttcgcct cgctttcgat cggggtgacg gtcgtcccgg
      181 agccggtccc ctggatgctg ttctcgttga ggtactggac ccgcgcgttg tcccggtggc
      241 gcaccccgaa gtccagcgcc agcgggtgct tcccccagga gagctggatc gcgacgccgg
      301 ccccggcgga ccagttgaag gtccagtcgc tgaagttcgt gctggaggcg aacgcgccac
      361 cgctcgaggt cccccgcgcc gaggtctggg tgtagaagta ggcggccccc accgagcccg
      421 acacgtacgg gcggatcgat ccgcgggcga ggtccacccg gaccaccggc gtgccgagcg
      481 tcacgatgtt gttggtggtg gtcacgtcgg cgtagaggcc ggggatggtc ggactgaggg
      541 ggacggtgcg ggtccggtcg ccgtagttca cgatcccgag ctcgaacccg accgtgaccc
      601 ggccgccgcg gtcgaggacc cagccccccc agccggtgag gccgaagccg tcgtgcacga
      661 agcgaccgaa gtcgccggtc ggcgccgcat attcgagcca gccacctgcc tggccccgga
      721 tcgggccggg cggcggttgc tggtcggtca cgtcgcgcaa ctgggcgcgg agcccggcgg
      781 ggcagagcag cagggcgagg acgccgagga tgtaccgcat acggcccacc ttgggagcga
      841 gcaggcggat gacctgcgcc cggggtcgtt acaagttccg cgccccagcg gtacgaggcg
      901 ccgccgcaac gggcccggcc gcgttgaaaa tgcctccggc ctcggctaag tttttggttt
      961 gcctgtggtt agctgcggag ggactgatgc cgacctatga gtacgcctgt ccggcagggc
     1021 atgcgttcga actgttccag aagatgtccg acaagccgcg ggccaagtgc ccggtctgtg
     1081 ggcgcgccgc cgtgcgccgg atctccggcg gcgcggggct cgtgttcaag gggagcggtt
     1141 tctacatcac cgactacggc aaggatggga aggggccgcg gaaggacccc gtcgactccg
     1201 cgcccgcgcc caaggccgat gtgaaacctg acaccaaggc cgagccgacg ccgaagccga
     1261 aggccaaggc gccgaaggcc ggcgagtgag cgagcagctg cggagcgagc tggcccggat
     1321 cgccgcgaag ctgggcggcg acggcgtgcc gttcgtcctc gaacgcccgc gcgaggatgc
     1381 gcacggcgac tgggccacga acctggcgct cctcctggcc caggcgcaga aggcgcggcc
     1441 gcgtgaggtg gccgagcgga tcgtggccga gctcgcgctc ccggccggcg tcgtggcgcg
     1501 gaccgagatc gccgggccgg gattcatcaa cttctggctc ggcaaggcgc agctggccaa
     1561 cgacctcgcg acgatcatcg gggcgggagc gcgctacggg cgccacacct tcggcgccgg
     1621 gcgcaaggtg aacatcgagt tcgtctccgc gaatccgacc ggcccgctcc acgtggggca
     1681 cggccgcggt gccgcattgg gagacgggat cgcggccctc ctcgagtgga ccggccacgc
     1741 cgtcacccgc gagttctaca tcaacgatgc gggcgtccag atcgacaagc ttgcgcgctc
     1801 cctctgggcg cgggtgcagg aagcggcggg acggaaggcg gagatccccg acggtgggta
     1861 tcacgggagc tacctctctg agtccgcggc caaggtgctg gagcgggaag gcaaggggtt
     1921 cgccgatctt ccggaggccg atggggtcgc ccggtgccgg gcgctggcgc tccggatgat
     1981 gcgggaagag caggacgagg acctgcgcca gttcggcgtc gtcttccagg cgatgacctc
     2041 cgagcaggcc ctgtacgacg gcgggaaggt cgaggcgggg ctcaagctcc tcgcggcgcg
     2101 gaagctcacc ttcgagcagg acggggcgct ctggctccgc accaccgact tcggcgacga
     2161 caaggaccgc gtcctccgga agtcggacgg ctcgctcacg tacctcgttc ccgacatcgc
     2221 ctaccatatc gacaagcacg agcgcggctt cgacctggcc atcgatgtct ggggcgccga
     2281 tcatcacggc tacatccccc ggatgcgcgc ggtgctcctg gcgctcggct accccgacgg
     2341 cttcttcgac gtggccctgg tgcagctggt caaggtggtg cggggcgggg aagaggtgaa
     2401 gatgtcgaag cggtcggggg agttcgtcac cctgcgcgac ctcttcgagg aggtcggcgt
     2461 cgacgcggcg cgctacttct tcctcatgcg ccggggtgac agccacctga tcttcgacat
     2521 cgacctggcc aagagccaga ccgacgagaa cccggtcttc tatgtgcaga tggcccacgc
     2581 ccggatgagc gggatcttcc gggtggccgg ccgcgagccc gcctcggccg acccggcggc
     2641 cgtcgatgtc gccgcgctct cggcgccgca ggaccaggac ctgctcaaga cgctcaccgt
     2701 cttccccgac gtggtggccg ccgcggcgcg cgcacgcgag ccgcaccgga tcacggcgta
     2761 tctcgaggac ctcgcgcgcc tggcccacaa ctggtaccat cactgccgcg tcctgggcga
     2821 gcctcccgcc gtggaagccg cccgcctggc gctcgcccgc gcggcgcgca cggtactcgc
     2881 caacgggctc accctgctcg gactcaccgc tcccgaccgg atgtgaacgt gaccctgctc
     2941 gtcgtcggct ccgtcgccct ggactcgatc tacacgccct tcggcgagac cgccgacgcg
     3001 ctgggcgggt cggcggtgtt cttctcggtg gcggcgtcgc tgctcacgac ggtcaaggtg
     3061 gtgggcgtca tcggctccga ctatccggtg gccgagctcg agcgcctggc gccccgcggc
     3121 atcgactgga gcggcgtcga acgggccgag ggggagagct tccggtggaa ggggaagtac
     3181 tcctatgacc tccagagccg ggagaccctc gagacccggc tcggcgtctt tgccgatttc
     3241 cagcccaagc tgccgccggc gttccggggc gcggagtacc tcttcctcgg caacatcgac
     3301 cccgagctcc agctcggggt gctctcccag atcgagcgcc cgaagctcgt ggcctgcgac
     3361 acgatgaact actggatcca gagcaagaag gacgtgctcc tccagctgct gaagcaggtg
     3421 gacatcctga tggtcaacga cagcgaggcg cgcgagctct cgggcgactg gaacatccac
     3481 cgcgccggcc ggtggatcct ggcccacggc cccagtcggg tggtcatcaa gcagggggag
     3541 tacggcgcgc tcctgatgga gccgggcggc accttcgcca tccccggcta tccgctcgag
     3601 gaggtgttcg acccgaccgg cgcgggcgac gccttcgccg gcgggttcat gggctacctc
     3661 gcccggagcg gcgacggcac cgacgcggcg ctccggcggg ccatggtcta cggttcggtc
     3721 atggggtcgt tcgcggtcga gcagttcggc acccgcggct tcgacgacgt ggacgatggc
     3781 gacgtcgaga accgggtcga cgggttccgc cagctcaccg cggtgcactt cccggggcgg
     3841 tccgcgtgac cgggcgccag tacgccgccg cgggggtcga cctcgacacc gccgaggacg
     3901 ccaaggcacg gatcggtcgc ctggtggccg gcacccggac cccgctctcc ctcggcgagg
     3961 tgggcgcttt cggcgggatg gtgcgcgtgc caccggggta ccgctcgccg gtcctggtga
     4021 tgagcaccga cggggtgggg accaaggtcc tcgtggcact cgaggcgggg cgcctcgaca
     4081 ccgtgggcga ggacctcgtc aatcactccg tcaacgacat cctggtccac ggggcccggc
     4141 cggtcgcgtt ccaggactac gtcgccggct ccgccctcac cgcggaactc atcgccgcgg
     4201 tggtcgaggg cgtcgcgcgc ggctgccgga accaccagat ggcgctcacc ggcggcgaga
     4261 ccgcgatgat gccggggctt taccagccgg gccacttcga cctggccggc accatcttgg
     4321 gcgtcgtcga ggaggaccag gccatccacg ggcaccgcat cgtgcccggc gacacgctcc
     4381 tcggctatgc gtcgtcagga ctacacacca acggctacac cctcgcgcgc cggatcctct
     4441 tcgatgtgat gaagctccaa ctgtcggccc cgctggcaga tacggggcgg acggtggtgg
     4501 atcacctgct cgcggtgcac gtgagctacg ccggcgcggt gatgccggtc atctcccggg
     4561 tgcacggtat cgcacatatc accgggggcg gcatcccggg caacctggtc cgcgtcttgc
     4621 ctcccgcgtg cgaggccgtg gtcgatgccg gcagctggac gctcccgccc ctcttccagg
     4681 tcctgcagcg ggcggggcag gtctccaccg aggagatgcg cgacgtgttc
//
LOCUS       DASUAP010000173         5008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_645609, whole genome shotgun sequence.
ACCESSION   DASUAP010000173 DASUAP010000000
VERSION     DASUAP010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645609"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFU45_04310"
     CDS             complement(<1..515)
                     /locus_tag="VFU45_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5358318.1"
                     /translation="MSHDPPGPGFWASVRESLRGSRHDYTTGPLGRAILLLAVPMVLE
                     MVMESVFAVVDIFFVGKVGPAAVATVGLTETIVIIVYTIAFGLSIGIAAMVSRRIGEK
                     DPDGAAHTAIQGIWLGALVSIPLAIAGVAAGPSLLALMGAGPDVLAIGTGYVRILLGG
                     NVVILLIFIINA"
     gene            complement(690..1517)
                     /locus_tag="VFU45_04315"
     CDS             complement(690..1517)
                     /locus_tag="VFU45_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358319.1"
                     /translation="MRLTRALSYVIAGAGGAVLLAAARPGRPSPARVLTVTTHDFAFE
                     APDSAPAGLTEIHLHNAGPSVHHLSIYRLGAGKTLADFMGALKPNAPFPTWATALGGP
                     NAVIPGGSSNATMVLTPAHYVLICFVPDSANRPHFMLGMEKELVVTGATKAVLPKADR
                     TVELSDYTFGLPATVRAGNQTWRFVNTAQQEHEALLVMLPPGKTIAEFGSWVDAGMQG
                     PPPGMPMGGISPLAPGGASEVALDLKPGNYAFICFLPDSKDGQPHFAHGMMRAFEVK"
     gene            1911..3119
                     /locus_tag="VFU45_04320"
     CDS             1911..3119
                     /locus_tag="VFU45_04320"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5358320.1"
                     /translation="MRTHSRIRSRFLTHCSLLALALAPALGAQAPAWPAFTHAFDSYA
                     LGDSVVGASVALVQDGRITLHHEYGFADRALHQRTDDRTIYHWGSITKTLTAVTVMQL
                     RDRGLLSLDDPVTRWIPELRQVHDTFGSIDSITIRMLLSHSSGFQDPTWPWTQGKPWE
                     PFEPTTWQQLVAMMPYEEVHFKPGSKYSYSNPAFIYLARIVELISGDPFETYLQKNVW
                     TPLGLTHSYFGTTPYWLAADRSDNYTVKRDSAGVEQVFTNGRDFNPGITRPNGAWNAP
                     LSDLAAWMGFLAGRAGGDSTLARTYATILPHADLVEMWRPRYDADAPGMPPEKVGMSF
                     FIFPRGADTLVGHTGEQAGFRSFMFINPRTGTGVVGVFNTRNDAESDKSSTGFNAIVE
                     QAIGLLRRGR"
     gene            3341..4822
                     /locus_tag="VFU45_04325"
     CDS             3341..4822
                     /locus_tag="VFU45_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU5358321.1"
                     /translation="MPHPPEESLDPKDPAEWEEFRTLAHRMVDDMVDHLSTLRQRPAW
                     QKTTPQVHTALDQPVPRQGQGMAAAYRDFVEFVRPYPVGNLHPRFWGWVMGNGTPFGM
                     MADMLAAGINANVGGFDQAPALVEEQVHAWLAELMGMPKTTTGLFVTGGSMANVIGLA
                     VGRHAQAARHGFDVRELGMQAQPGRMTCYGSVETHSWARKAVEFLGLGNAAYRKIPVD
                     REYRIDLAALEARVREDRAAGELPFCVIGSAGTVNTGATDDLRALAAFARREGLWFHV
                     DGAFGALARLSPSLRPVVDGIEEADSLAFDLHKWGYLPIECACVLVRDGAAHRAAFAT
                     TASYLTASPRGVNPGPMIFADRGLDLSRSFKALKVWLSFKAHGVEKFGRLIEQNVAQA
                     RHLAARVTASPDLELLAPVPLNIVCFRYAPRHAPADRLDAINEEILIQLQERGIAVPS
                     GTRIGGRFAIRVANTNHRSRLEDFDALAEAVVEIGRSVAGQGG"
BASE COUNT          852 a         1728 c         1697 g          731 t
ORIGIN      
        1 gcgttgatga tgaagatgag gaggatgacg acgttgccgc cgagcaggat gcggacgtag
       61 ccggtaccga tggccaggac gtcgggcccg gcgcccatca gcgccaggag cgacggcccg
      121 gccgcgaccc cggcgatggc cagcgggatc gacaccaggg cgccgagcca gatcccctgg
      181 atggcggtgt gggccgcacc gtcggggtcc ttttctccga tccggcggga caccatcgcc
      241 gcgatcccga tcgagaggcc gaacgcgatg gtgtagacga tgatgacgat ggtctcggtg
      301 agcccgaccg tggcgacggc cgcggggccc accttgccca cgaagaagat gtccaccacc
      361 gcgaacaccg actccatgac catctccagc accatcggca cggccaggag gaggatggcg
      421 cgcccgagcg gccccgtggt gtagtcgtgg cgggagccgc ggagcgactc gcggacggag
      481 gcccagaagc cggggccggg agggtcgtgg gacatgaggg gggagaaggg taaacgggcg
      541 ggattcaagg gaaaagtgaa gagggaagag ggaaaaggga agaggaaaaa gtgaagaggg
      601 aaaaggaaag agggagcgcc gtagacctgc acgcaccctc ttcactattc acttttccct
      661 tttccctttt cactcttcac ttcccatcgc tacttcactt cgaacgccct catcatcccg
      721 tgcgcaaagt gcggctggcc gtccttggag tcgggaagga agcagatgaa ggcgtaattc
      781 cccggcttga ggtcgagcgc gacttcgctg gcgccacccg gcgcgagcgg cgagatcccg
      841 cccatcggca tccccggcgg cgggccctgc atcccggcat ccacccacga gccgaactcg
      901 gcgatggtct tgcccggggg gagcatcacc aggagcgcct cgtgctcctg ctgtgccgtg
      961 ttgacgaacc gccacgtctg gttcccggcg cgcacggtgg ccgggagccc gaacgtgtag
     1021 tcggacagct cgacggtccg gtcggccttc gggagcaccg ccttcgtggc gcccgtcacc
     1081 accagctcct tttccattcc gagcatgaag tgcggccggt tggcggagtc gggcacgaag
     1141 cagatgagca cgtagtgggc cggcgtgagc accatggtcg cgttcgacga cccgcccggg
     1201 atgacggcgt tgggcccgcc gagcgcggtg gcccacgtcg ggaacggggc gttcggcttg
     1261 agggcgccca tgaagtcggc cagcgtcttc cccgccccga gccggtagat cgacagatga
     1321 tgcacgctcg gccccgcgtt gtgcaggtgg atctcggtga gccccgccgg cgcggagtcc
     1381 ggcgcctcga acgcgaagtc gtgggtcgtc accgtcagca cccgcgcggg cgacggacga
     1441 cccggtcgcg ccgcggcgag caacacggcg ccgccggcgc ctgcaatgac ataggagagg
     1501 gctcgcgtga gtcgcatcgg atgaacctcg tcgaatgtgg ggaggccggg ggatctcggg
     1561 ccacagggcc gtagtcccga ggggcggaga gattacttcc tggagccgga tcggacaagg
     1621 gtgactgttg ggacgggggc cgggactaag ggtgtcgtta gaaaagcgag ggaaagcgtt
     1681 ggaaagcggg ggaaggttgg agaaggtgac gggaatgcga ccggagcgct gtccgccatc
     1741 ggagtcatcc tgagggcgcg gagcgaccga aggatctctc gggtggacgc gaagagatcc
     1801 ttcggggcct gaaggcccct caggatgact ccgatggcgg gcggctcccc gcccgggttg
     1861 tccgcgaagc ccgccctacc cgccttgccc acccgccgta tcttttcgga atgcgcacac
     1921 actcccgcat ccgctcccga ttcctcacac actgctccct gctggcgctc gccctcgcgc
     1981 ctgccctcgg tgcccaggcc cccgcctggc ccgcattcac ccacgcgttc gacagctacg
     2041 ccctgggcga cagcgtcgtc ggcgccagcg tggcactggt gcaggacggg cggatcaccc
     2101 tccaccacga atacggcttc gccgaccggg cactgcacca gcgcaccgac gaccgcacga
     2161 tctaccactg gggttcgatc accaagacgc tcaccgccgt gacggtgatg cagcttcgcg
     2221 atcgcgggct cctctccctc gacgacccgg tcacccggtg gatcccggag ctccgccagg
     2281 tccacgacac cttcggctcg atcgactcga tcacgatccg gatgctgctc tcccactcct
     2341 ccggcttcca ggaccccacc tggccctgga cccaggggaa gccgtgggaa cccttcgagc
     2401 ccacgacatg gcagcagctc gtggcgatga tgccgtacga ggaggtccac ttcaagccgg
     2461 gatcgaagta ctcctacagc aacccggcgt tcatctacct cgcgcggatc gtggagctca
     2521 tttccggcga cccattcgag acctacctcc agaagaacgt ctggacgcca ctcggcctga
     2581 cccacagcta cttcggcacc acgccgtact ggctcgccgc cgaccggtcc gacaactaca
     2641 ccgtcaagcg cgacagcgcg ggcgtcgagc aggtcttcac caacgggcgc gacttcaacc
     2701 ccggcatcac ccgccccaac ggtgcctgga acgctccgct cagcgacctc gcggcgtgga
     2761 tgggattcct ggcgggccgg gcaggcgggg actcgacact cgcgcggacc tacgccacga
     2821 tcctccccca cgctgacctg gtcgagatgt ggcggccgag atatgacgcc gatgcgccgg
     2881 ggatgccgcc ggagaaggtc ggaatgagct tcttcatctt cccccgcggc gccgatacgc
     2941 tcgtgggcca cacgggagag caggcaggct tccggtcgtt catgttcatc aacccccgca
     3001 ccggcaccgg ggtcgtcgga gtgttcaaca cccggaacga tgccgagtcc gacaagtcgt
     3061 cgacggggtt caacgcgatc gtggagcagg cgatcggcct tctccgtcga ggccgatgaa
     3121 gctgacgcaa cgcggcccaa catcggggtc atcccgaggg agcgaagcga ctgagggatc
     3181 tctcgggtgg acgcgaagag atccttcggt cgctccgctc cctcaggatg actccgatgg
     3241 cacgccacgc tccactggcc ccgcccgcac catgactcct ttacgttccc accccgtagg
     3301 gcgcctacta tatagaagca gtccacgccc aggagccgcc gtgccccacc cgccggaaga
     3361 atcgctcgac cccaaagatc ccgccgagtg ggaggagttc cgcacgctcg cccaccggat
     3421 ggtggacgac atggtggacc acctctccac cctgcgccaa cgccccgcct ggcagaagac
     3481 cacgcctcag gtccacaccg ccctcgacca gcccgtcccc cgccagggcc aggggatggc
     3541 cgccgcctat cgcgatttcg tcgagttcgt ccgcccctac ccggtgggca acctccaccc
     3601 ccgcttctgg ggatgggtga tgggcaacgg caccccgttc gggatgatgg ccgatatgct
     3661 cgccgccggg atcaacgcca acgtcggcgg gttcgaccag gcgccggcgc tggtggaaga
     3721 gcaggtccac gcctggctcg ccgagctgat gggaatgccg aagaccacca ccgggctctt
     3781 cgtgaccggc ggctcgatgg ccaacgtcat cgggctcgcg gtagggcgcc acgcccaggc
     3841 cgccaggcac gggttcgacg tccgcgagct cggcatgcag gcacagcccg ggcggatgac
     3901 ctgctacggc tcggtcgaga cccacagctg ggcccgcaag gcggtcgaat tcctcgggct
     3961 cggcaacgcc gcctaccgca agatccccgt ggatcgggaa taccggatcg acctcgcggc
     4021 gctcgaggcg cgggtccggg aggatcgcgc ggcgggagag ctccccttct gcgtgatcgg
     4081 gagcgccggc accgtcaaca ccggcgccac cgacgacctc cgggcgctcg cggcctttgc
     4141 gcggcgcgaa gggctctggt tccacgtcga cggcgcgttc ggagcgctgg cccgcctgtc
     4201 gccttcgctc cgaccggtcg tcgacgggat cgaggaggcg gattcactcg ccttcgacct
     4261 ccacaagtgg ggctacctcc ccatcgagtg cgcctgcgtc ctcgtccgcg acggggcggc
     4321 ccaccgcgcc gcctttgcca cgactgcgag ctacctcacg gccagtccgc gcggcgtcaa
     4381 cccggggccg atgatctttg ccgaccgcgg gctcgacctg agccggagct tcaaggcgct
     4441 caaggtgtgg ctctccttca aggcgcacgg ggtcgagaag ttcggccgcc tgatcgagca
     4501 gaacgtggcc caggcccggc acctcgcggc acgggtcacc gcctcccccg acctcgaact
     4561 cctggcgccg gtgccgctca acatcgtctg cttccgctat gctccccggc acgcccccgc
     4621 cgaccggctc gacgcgatca acgaggagat cctgatccag ctccaggagc gcggcatcgc
     4681 cgtcccctct ggcaccagga tcggcggccg ctttgccatc cgggtcgcca acaccaacca
     4741 tcggagccgg ctggaggact tcgatgcgct ggcggaggca gtggtggaga tcgggaggag
     4801 cgtggcgggg cagggcgggt agggcgggta cggcggacaa ggcagttgag acgggcgggt
     4861 acggcggacg aagcgggcga ggtgggcggg tagggcggac ggagcgcggt gagcgaacgg
     4921 agtgtcatcc cgagcggatg cgagggatct ttcccgcata gctgtgcgct ggccgccagc
     4981 gtcgatcgct ccggagaaga ggcccgcg
//
LOCUS       DASUAP010000174         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_223309, whole genome shotgun sequence.
ACCESSION   DASUAP010000174 DASUAP010000000
VERSION     DASUAP010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223309"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VFU45_04330"
     CDS             <1..948
                     /locus_tag="VFU45_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358322.1"
                     /translation="LSQARQQLQKKWRADVTSELDRALAETARLSTSELALSGDFERG
                     EPVATLRGRQAAIVEGAQRVIAELQDASGRNAIVSPRLAIAMTQALVDMQRALGALSS
                     AVPDLAAGGGRAGDAVDDLNAAAYLLLRNRDDVAGSGSGSGMAEAMARMRELAGQQGS
                     LGDAAAGLLPSFTSGRDGAQVQALGGRERAIADQLERLRAGGQLPGAGAMADEAKDLA
                     RRLEGGQLDRTSVERQQRLFRRMLDAGRTLQGNEDDPDRPRVSRAAVGDSVHVPPPLR
                     RLPGADAPRMPSWSDLERYSPEERRLVADYFQRLLAGGR"
     gene            945..2669
                     /locus_tag="VFU45_04335"
     CDS             945..2669
                     /locus_tag="VFU45_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358323.1"
                     /translation="MKAVRFVAALLFLAGPVAAQADTSLARAFDLERRGAFAGAADAY
                     RGLLQREPGNLAALLGLQRSLTPLGREGELGPLLAPVLRAAPTPAVFDIALRAWLAVG
                     ERDSARSVVVQWARVDPDRLAPYREWGDLLLSRRDAAGARQAYEAGRAASGDSASFAV
                     ELAQAETLDGDLVSAAAEWARAIGQFPAMRPAAVASLARAPAAQRGRILHALDGAGPA
                     GRSVATLLTAGWGDAAGAVDRVAADIASGSGDIGILQALVGELRGSTARPARGALGRA
                     LELLAARQSGPAIPSLRLEAARAYADGGDRASARRMLSLVLADPGASAALGPGSARIV
                     IEVLIGEGDLAEAERQLDHPAAGLAPDDARALRRRLALALAQAGELDRAQRLLVADSS
                     VETAAIQGRLFLYMGDLANARRALKWAGPFAGDRAEATERVRLLALLQPIEADTLRAL
                     GAGLWALDRGDTVGAVTLLTPLADSLPPGKGGAETAFLLGRVSVARGDTVGAARWLRR
                     AVSDSVGATSPAAEYALARFLAARGHRGEAIASLQHLILAWPTSAVVPEARRLLEQLQ
                     APAPGAAT"
     gene            2666..3958
                     /locus_tag="VFU45_04340"
     CDS             2666..3958
                     /locus_tag="VFU45_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358324.1"
                     /translation="MRRRDFVRGAAAVGVAGLLPAGLRGWTGGSADLLLVPMDDAQDD
                     HLKAYGVTFRMLQRGGRGEWLLNYRGGSFLLPGDAATARDAALAGVRVEPLSNGEAAA
                     IKAGMARDNADAIPLEKVPRVAVYVPPDAPPWDDAVTMALQYAGIDFAKVWDAEVMGD
                     GLVAYDWMHLHHEDFTGQYSKFYLNYANVPWLREMVRMQLAMARKLGFADVPAAKRAV
                     ARRIRSFVERGGFLFAMCTATETLDLALASEHVDIAASFADGTPMDPQASSKMDWSQA
                     LAFQDAELELSPTVPEFSDIDGHQVNSPTRRQPLGSFTLFPFSAKIDPVNTMLVQNHR
                     NVIPDFYGITTSFRRDRVKPQVTVLAEEPGAPWVKYIHGERGEGTWTFFGGHDPEDPE
                     HQIGDPPTDLSLHPHSPGYRLILNNVLFPAAKKRELKT"
     gene            4030..>4771
                     /locus_tag="VFU45_04345"
     CDS             4030..>4771
                     /locus_tag="VFU45_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEU5358325.1"
                     /translation="MPRTHLLLALALSAAAPLAAQAPVITPDGDPSVRSDTIYALAVD
                     PAKHPGESAAYLLDDGVIRIDQDGHISRTYRQVIQILTEDAVKPEQEWSFSWHPGYQT
                     FRLNWIRVVKPDGTVISAAPSQQQVTDVPAEMGDPVYSDEKVLRVSLSGVAVGTEVDY
                     STTTEDTKPFLKGDFLFSWGISNTLTTRRSRYIVEAPAGLKLRIDERNLDFARRTTVH
                     DGRQTLVWAAADVPRVKEESFADYDTNAV"
BASE COUNT          628 a         1682 c         1807 g          654 t
ORIGIN      
        1 ctgtcccagg cgcggcaaca gctccagaag aagtggcgcg cagacgtgac gtcggagctc
       61 gaccgggcgc tggcggagac cgcccggctc tcgacatcgg aactggccct gagcggggac
      121 ttcgaacggg gggaaccggt cgcgaccctg cgcgggcgtc aggcggcgat cgtggaggga
      181 gcgcagcgcg tcatcgccga gctgcaggac gcgtcgggcc ggaatgccat cgtctcacct
      241 cggttggcca tcgcgatgac gcaggcgctg gtcgatatgc agcgcgcgct gggcgcgctg
      301 tcgagcgcgg tgcccgacct cgcggcgggt ggcgggcgtg ccggggacgc ggtggatgac
      361 ctcaacgccg ccgcgtacct cctgctccgc aatcgcgatg acgtggcggg ctcgggctcc
      421 ggctcgggta tggcggaggc gatggcccgg atgcgggagc tggccggaca gcaggggagc
      481 ctcggggacg cggccgccgg attgctcccg tcgttcacca gcgggaggga cggcgcgcag
      541 gtgcaggcgt tgggcgggcg cgagcgcgcc atcgccgacc aattggagcg cctgcgggcg
      601 gggggccagc tgccgggggc cggcgccatg gccgatgagg ccaaggatct tgcccgccgc
      661 ctcgagggcg gccagctcga tcgcacgtcg gtggagcggc agcagcggct cttccggcgg
      721 atgctcgacg cgggccgcac cctgcagggg aacgaggacg atccggatcg tccccgggtg
      781 agccgcgccg cggtgggcga cagcgtccac gtcccgccgc cgctccgccg cctcccgggt
      841 gccgacgcgc cccggatgcc gagctggagc gatctcgagc ggtattcgcc tgaggagcgg
      901 cgcctcgtgg ccgactactt ccagcgtctg ctggcggggg gccgatgaag gccgtccgtt
      961 tcgtggcggc cctgctgttc ctggccggcc cggtcgcggc gcaggcggac acgtcgctgg
     1021 cccgggcgtt cgacctcgag cgccgggggg cgttcgccgg ggcggcggat gcataccgcg
     1081 gattgctgca gcgggaaccg gggaaccttg cggcgctcct gggactgcag cggtcgctca
     1141 cgccgctggg ccgggaaggg gagctcggac cgctcctggc cccggtgctt cgtgccgcac
     1201 cgacgcccgc ggtgttcgac atcgcgctcc gggcgtggct tgccgtcggc gagcgcgaca
     1261 gcgcccgaag tgtcgtggtg caatgggcgc gggtcgaccc ggaccgcctg gcgccgtatc
     1321 gggagtgggg tgacctgctc ctctcccgcc gggacgcggc cggggcgcga caggcgtacg
     1381 aagccggccg ggccgcgagc ggcgacagcg ccagcttcgc cgtggagctc gcgcaggcgg
     1441 agacgttgga cggcgatctc gtgtccgccg cggcggagtg ggcgcgtgcc atcggccagt
     1501 tcccggcgat gcgcccggcg gcggtggcct cgctcgcccg ggcgcccgcg gcccagcggg
     1561 gccgcatcct ccacgccctc gatggtgccg gcccggccgg caggtcggtt gcaacgctcc
     1621 tgaccgcggg gtggggcgat gccgcgggcg cggtcgaccg cgtcgcggcg gacatcgcgt
     1681 cgggctcggg agacatcggc atcctgcagg cgctcgtcgg ggagttgcgg gggagcacgg
     1741 cacgaccggc gcgtggcgcc cttggccgcg cgctcgagct cctggccgcg cgccagtcag
     1801 gtcctgcgat cccgtccctc cgcctcgagg cggcccgcgc ctacgccgac ggcggcgacc
     1861 gggcgtcggc tcggaggatg ctctcgctgg tcctggcgga tcccggcgct tcggcggcgc
     1921 tcggaccggg gtcggcccgg atcgtgatcg aagtcctcat cggcgagggc gatctggccg
     1981 aggcggagcg gcagctggat caccccgcgg ccgggctggc gcccgacgat gcgcgtgcgc
     2041 tccgccggcg gctggcgctg gccttggcgc aggcggggga gctcgaccgg gcccagcgcc
     2101 tcctggtggc cgacagctcg gtggagaccg ccgcgatcca ggggcgccta ttcctttata
     2161 tgggtgacct ggccaatgcg cgccgggcgc tcaagtgggc cggccccttc gccggcgacc
     2221 gggccgaggc caccgagcgc gtccgcctgc tcgcgctcct gcagccgatc gaagcggaca
     2281 ccctgcgggc gctcggcgcg ggtctgtggg cgctcgaccg cggagacacg gtgggcgcgg
     2341 tgaccctgct gacgcccctg gccgattcac tgccgcccgg gaagggcggg gcggagaccg
     2401 cgttcctcct cgggcgggta tcggtggcgc ggggggatac ggtcggcgcg gcgcgctggc
     2461 tccggcgggc ggtctccgac agcgtcggcg ccacctcgcc ggcggcggag tatgcgctcg
     2521 cgcggttcct cgcggcgcgc ggccaccgcg gcgaagcgat cgcctcgctg cagcacctca
     2581 tcctggcctg gccgacctcg gcggtggtgc cggaagcgcg ccggctcctc gaacagctcc
     2641 aggcgccggc gccgggagcg gccacatgag gcggcgcgac ttcgtgcggg gtgcggcagc
     2701 ggtcggggtg gcgggactgc tcccggccgg gctccgaggg tggaccggcg ggtcggccga
     2761 cctcctcctg gtgccgatgg acgatgccca ggacgaccac ctcaaggcgt acggcgtcac
     2821 cttccggatg ctgcagcggg gcgggcgcgg cgaatggctg ctcaactatc ggggcggctc
     2881 cttcctgctg ccgggcgacg cagcgaccgc gcgtgacgcg gcgctcgcgg gggtgcgggt
     2941 ggagccgctc tcgaacgggg aggccgccgc gatcaaggcc gggatggcgc gcgacaacgc
     3001 cgacgccatc ccgctcgaga aggtgccgcg ggtggcggtg tacgtgccgc ccgatgcgcc
     3061 gccctgggat gatgcggtca cgatggcgct gcagtacgcc ggcatcgact tcgccaaggt
     3121 gtgggacgcc gaggtgatgg gcgacgggct cgtggcctac gactggatgc acctgcacca
     3181 cgaggacttc accgggcagt actcgaagtt ctacctcaac tacgccaacg tgccctggct
     3241 gcgggagatg gtgcggatgc agctcgcgat ggcgcggaag ctcgggttcg ccgacgtgcc
     3301 ggcggccaag cgcgccgtgg cccggcggat ccgctcgttc gtggagcggg gcgggttcct
     3361 cttcgcgatg tgcaccgcca ccgagacgct cgacctggcg ctcgcgagcg agcatgtgga
     3421 catcgcggcg agctttgccg acgggacgcc gatggatccg caggcatcgt cgaagatgga
     3481 ctggagccag gccctggcgt tccaggacgc cgagctcgag ctgagcccga cggtgccgga
     3541 attctccgac atcgacgggc accaggtcaa ttcgccgacc cggcgccagc ccctcgggtc
     3601 gttcacgctc ttcccgttca gcgccaagat cgacccggtg aacacgatgc tggtgcagaa
     3661 ccaccggaac gtcatccccg acttctacgg catcacgacc tccttccgcc gcgaccgggt
     3721 caagccgcag gtgacggtgc tcgcggagga accgggggcg ccgtgggtca agtacatcca
     3781 cggggagcgc ggggagggga cgtggacctt cttcggcggt cacgacccgg aggatcccga
     3841 gcaccagatc ggcgacccgc ccaccgacct ctcgctccat ccgcactcgc cggggtaccg
     3901 gctgatcctc aacaacgtcc tcttcccggc ggcgaagaag cgggaactga agacctgaac
     3961 ggacgcaccc ggccacgcta ttattgtcga tgaccccctc gggctcccac ccttctcccg
     4021 gtccggtcca tgccccgaac gcacctgctg ctcgccctgg cgctcagcgc cgccgcgccg
     4081 ctcgccgccc aggcgccggt catcactccg gacggcgacc cgtcggtccg ctccgacacc
     4141 atctatgcgc tcgcggtgga cccggcgaag cacccggggg aatcggccgc gtacctgctc
     4201 gacgacggcg tcatccgcat cgaccaggac gggcacatct cccggaccta ccggcaggtg
     4261 atccagatcc tgaccgagga cgcggtcaag ccggagcagg agtggtcgtt ctcctggcac
     4321 ccgggatacc agacgttccg gctcaactgg atccgcgtgg tgaagcccga cggcacggtc
     4381 atctcggccg cgccctcgca gcagcaggtg acggacgtgc cggcggagat gggcgatccg
     4441 gtgtattccg acgaaaaggt gctccgcgtc tcgttgagcg gcgtggcggt cggcaccgag
     4501 gtcgactaca gcaccaccac cgaggacacc aagccgttcc tcaagggcga cttcctgttc
     4561 tcctggggga tctccaacac cctcacgacc cgccggtcgc gttacatcgt cgaggccccg
     4621 gcgggcctca agctgcggat cgatgagcgc aacctcgact ttgcccgccg caccacggtc
     4681 cacgacggac gccagaccct ggtgtgggcc gccgccgacg tgccgcgggt caaggaggag
     4741 tcgttcgccg actacgacac caacgcggtg g
//
LOCUS       DASUAP010000175         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_304709, whole genome shotgun sequence.
ACCESSION   DASUAP010000175 DASUAP010000000
VERSION     DASUAP010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304709"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2975)
                     /locus_tag="VFU45_04350"
     CDS             complement(<1..2975)
                     /locus_tag="VFU45_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5358326.1"
                     /translation="MTSILRGRRAEAAPEAPPFPGILSALDGSGAVVAMETAASEAAG
                     AYPITPSTQMGEGWAAAVAEGKENVNGRRLLFFEPEGEHAAAAVTAGMSMAGFRAANF
                     SSGQGIAYMHESLYAAVGKRLTYVLNVAARAITKQALNVHAGHDDYHAVDDTGFFQLF
                     AKDVQEAADLDLVAHRIAELSLNPGICAQDGFLTSHVIESLRLPERDLIKAYLGDPAD
                     RIPSPTPAQRMVFGDTRRRIPELFDLDDPAMLGVVQNQDSYAQGVAAQRPFYFDHIPA
                     LTDQAFEEFARLTGRRYARIGAWRLEDAEYVIVGQGSVVANAEAVADHLRAERRVRVG
                     VLNLTMFRPFPADLVSHALAGKKGVVVLERTDQPLAVDPPLLREIRAAMSHGVENGRA
                     SSAGGPRHPGVAALRPDQVPDFFSGCFGLGSRDLQPGDIIAAVDNMLPGGARRRQFYL
                     GIDFIRRDTRLPKLQIWQERMLDGYPGVGELSLRSAGDLDLLPEGSTAVRIHSVGGWG
                     AITMGKNLAMTAFELFGMHIKANPKYGSEKKGQPTTFYAVLAHEPVRLNCELKHVDVV
                     LSPDPNVFRHSDPLAGLAEGGVFVIQSDLSPEAFWLTLPASARATIRDRKIKVFILDA
                     FAIARAEASDAELRYRMQGAAFLGAFFSTSSLLRQEESTEARLFDGIRAQFTKKFGHR
                     GGSVVEDNLRVIRRGFDEVREVTPAEVAATDTVAAVPHIPAALDVVDAAPGIANPGRF
                     WEQVCTLCALGQDGIADPFAAVSAIPAATGTVRDMSGVRMEVPQFVAANCTGCAQCWT
                     QCPDAAIPGLVSAPDEVLAALVGALEDAACDPLRPLLKPWGRETQRLLAREPALAVTD
                     AFARAYVAVADKMGWDAERRAATDRLVARVQERIAEFPLARTRPFFDAVESKQKGTGG
                     LLSITVNPDACKGCNLCVEVCPDGALVTVRQDDATLARLRRNWAAWQRLPDTDDRYVN
                     VSGLDEGIGVLPSLLLKKAT"
     gene            complement(2972..>3446)
                     /locus_tag="VFU45_04355"
     CDS             complement(2972..>3446)
                     /locus_tag="VFU45_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257395005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5358327.1"
                     /translation="AAAAAVAGGGKGCAWGCIGLADCERICDFDAITMNDAGLPVVDP
                     VKCTACGDCVDACPKGLFTLLPVDYHLFVQCRNLVGGDDALAQCRVACTACGKCVQDA
                     APGLISVASGVAVVDYGRIALEDRGAVERCPTGAIVWLDGAQFAAGHSLAGSVGA"
BASE COUNT          485 a         1223 c         1255 g          483 t
ORIGIN      
        1 gtcgccttct tgaggaggag cgaaggcagc acgccgatcc cctcgtcgag gccggacaca
       61 ttgacgtacc ggtcatcggt gtcggggagc cgctgccagg cggcccagtt ccggcggagc
      121 cgcgccagcg tggcgtcgtc ctgcctgacg gtcacgagcg cgccgtcggg gcacacctcg
      181 acgcagaggt tgcatccctt gcacgcgtca gggttgacgg tgatcgacag gaggccgcca
      241 gtccccttct gcttcgactc gacggcgtcg aagaaggggc gggtgcgggc gagggggaac
      301 tccgcgatcc gctcctgcac ccgggccacg agacggtcgg tggcggcgcg ccgttcggcg
      361 tcccatccca tcttgtcggc gaccgcgacg tacgcccgcg caaaggcatc ggtgaccgcg
      421 agcgccggct cgcgggccag gagccgctgg gtctcccgcc cccacggctt gagcaggggg
      481 cggagcgggt cgcaggcggc gtcctcgagg gccccgacca gcgcggcgag cacctcgtcg
      541 ggagcgctga cgagcccggg gatcgcagcg tcgggacact gggtccagca ctgggcgcac
      601 ccggtgcagt tggcggccac gaactgcggc acctccatcc ggacgccgct catgtcgcgc
      661 acggtgccgg tggcggcggg gatggcgctg acggcggcga aggggtcggc gatgccgtcc
      721 tgccccaggg cgcagagggt gcagacctgc tcccagaagc gccccgggtt ggcgatcccc
      781 ggcgcggcgt ccaccacgtc gagcgcggcg gggatgtgcg gcaccgcggc cacggtgtcc
      841 gtcgccgcca cctccgccgg cgtcacctcg cgcacttcgt cgaaaccgcg gcggatgacc
      901 cggaggttgt cctccaccac gctgccgccg cggtggccga acttcttcgt gaactgggcc
      961 cggatcccgt cgaagagccg cgcttcggtg ctctcctcct gccgcaggag cgatgaggtg
     1021 ctgaagaagg cgccgaggaa cgccgcgccc tgcatccggt agcggagctc ggcgtcggac
     1081 gcctcggcgc gcgcgatggc gaacgcgtcg aggatgaaga ccttgatctt ccggtcccgg
     1141 atggtcgcgc gtgcgctcgc cggcagggtg agccagaacg cctcggggga gaggtcgctc
     1201 tggatcacga agacgccgcc ctcggccagg cccgccagcg ggtcgctgtg gcggaagacg
     1261 ttggggtcgg gggagagcac cacgtcgacg tgcttcaatt cgcagttgag ccgcaccggc
     1321 tcgtgagcga gcaccgcgta gaacgtcgtg ggctgcccct tcttttccga gccgtacttg
     1381 gggttggcct tgatgtgcat cccgaagagc tcgaaggcgg tcatcgcgag gttcttcccc
     1441 atcgtgatgg cgccccaccc gcccaccgaa tggatccgca ccgcggtgga gccttcgggc
     1501 aggaggtcca gatcccccgc ggagcggagc gagagctcac ccacgccggg gtagccgtcg
     1561 agcatccgct cctgccagat ctggagcttg gggagtcggg tgtcgcgacg gatgaagtcg
     1621 atgccgaggt agaactggcg ccgccgcgcg ccgccgggga gcatgttgtc gaccgcggcg
     1681 atgatgtcgc cgggctggag gtcgcggctc ccgaggccga agcagccgga gaagaagtcg
     1741 ggcacctggt cgggacggag cgccgccacg ccggggtgcc gcggtccgcc ggcggagctg
     1801 gcgcggccgt tctcgacgcc gtgggacatc gccgcgcgga tctcgcgcag gagcggcgga
     1861 tcgacggcga gcggctggtc ggtccgctcg aggaccacga cgcccttctt cccggccagt
     1921 gcgtggctca ccaggtcggc cgggaagggg cggaacatcg tcaggttgag cacgcccacc
     1981 cgcacccgcc gctccgcgcg caggtggtcg gccaccgcct cggcattggc caccaccgag
     2041 ccctggccga cgatcacgta ttcggcgtcc tccaggcgcc aggcgccgat gcgggcgtaa
     2101 cgccgccccg tcagccgggc gaactcctcg aacgcctggt cggtgagggc ggggatgtgg
     2161 tcgaagtaga agggccgctg ggccgcgacc ccctgggcgt agctgtcctg gttctgcacc
     2221 acgccgagca tcgcgggatc gtcgaggtcg aacagctccg ggatgcggcg gcgggtgtcg
     2281 ccgaagacca tccgctgcgc cggggtcggg ctcgggatcc ggtcggcggg atcgccgaga
     2341 taggccttga tgaggtcgcg ctcggggagc cggagcgatt cgatgacgtg ggacgtgagg
     2401 aatccgtcct gggcgcagat ccccgggttg agcgagagct ccgcgatccg gtgggccacg
     2461 aggtcgaggt cggcggcttc ctggacgtcc ttggcgaaga gctggaagaa cccggtgtcg
     2521 tccaccgcgt ggtagtcgtc gtggcccgcg tggacgttga gtgcctgctt ggtgatggcc
     2581 cgcgcggcga cgttgagcac gtaggtcagg cgcttgccga ccgccgcgta gagcgattcg
     2641 tgcatgtacg cgatgccctg cccgctcgag aagttggcgg cgcggaaccc cgccatcgac
     2701 atcccggccg tcaccgcggc ggccgcgtgc tccccctcgg gctcgaagaa gaggagccgc
     2761 cggccattga cgttctcctt cccctctgcc accgcggccg cccatccttc gcccatctgg
     2821 gtcgacggcg tgatgggata cgcgccggcc gcctcgctcg ccgcggtctc catcgcgacc
     2881 acggcgccgc tcccatcgag cgccgagagg atccccggaa aggggggcgc ctcgggcgcc
     2941 gcctcggccc gccggccccg caagatcgac gtcatgctcc cacgctcccg gccaatgagt
     3001 gccccgctgc gaactgcgcc ccgtcgagcc agacgatggc cccggtgggg cagcgctcca
     3061 ccgccccccg gtcctccagc gcgatgcggc cgtagtccac caccgccacc ccgcttgcga
     3121 cgctgatcag ccccggcgcc gcgtcctgca cgcacttgcc gcaggcggtg cacgccacgc
     3181 ggcactgggc cagcgcgtcg tcgcccccca ccaggttccg gcactgcacg aagaggtgat
     3241 agtccaccgg cagcagggta aagagcccct tggggcacgc atccacgcag tcgccgcagg
     3301 cggtgcactt gacgggatcc accaccggca ggccggcgtc gttcatcgtg atcgcgtcga
     3361 aatcgcagat gcgctcgcag tcggccaggc caatgcaccc ccaggcgcac cccttgccgc
     3421 cgcccgccac cgccgccgcc gcggcg
//
LOCUS       DASUAP010000176         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_986245, whole genome shotgun sequence.
ACCESSION   DASUAP010000176 DASUAP010000000
VERSION     DASUAP010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986245"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFU45_04360"
     CDS             <1..615
                     /locus_tag="VFU45_04360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEU5358328.1"
                     /translation="LRSRVRLARLERGADLLRRKREALVAELFRLARPASDARGRIEQ
                     AAEEAYVALLPALTEHGAAGLRALGWPARDYPVELAPGMVWGVPASRIVSRPPVPRTL
                     GARGTAPAAVGGSAVRAADRFERLVEELLEAAPREMLLRRIGEALARTSRQVNTLERR
                     LAPRLARDIVRVRQVLEEREREERVRLDGLLRRRRSRPAAEARP"
     gene            735..1289
                     /locus_tag="VFU45_04365"
     CDS             735..1289
                     /locus_tag="VFU45_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEU5358329.1"
                     /translation="MTEDPAANGLATRRRFLKWATAISSLAAAALAGVPVVRAFVSPA
                     FRKPVAKRWVKLGEADLFDVGAPTRVDFAETIEDAWVEQRTLRSVWVYSDDGEAFTVY
                     NGRCTHLGCAYRFDKTADPQYHSQENVFHCPCHHGVFDLKTGAVLAGPPPRPLDTLET
                     KIEDGMLYAAYEDFRVGIAERVTT"
     gene            1286..2758
                     /locus_tag="VFU45_04370"
     CDS             1286..2758
                     /locus_tag="VFU45_04370"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU5358330.1"
                     /translation="MKGVLRWLDERIDLAGLKRALLDREVPARLTWWHTLGSATLTVF
                     LLQLVTGIVLAAYYSPSPDHAYDSLQFIGRQVSSGALLHGIHHWGASAMVVLLLAHMI
                     RVFSVGAYKYPREINWLVGVGLFFVVMAFSFTGYLLPWDQKAYWATAVGTNIAGTTPG
                     IGGFLVSLLRGGGELGSATLTRFYSFHVLWLPLFLGAMLFVHLAILVRQGIAPRPAAL
                     EAGAPRRTDAADYPGYYRAAYADTKKAGMRFWPDVIGKDAMVALGVVALIVLLAAAFG
                     APLEAPADPTDSAYVPRPEWYFLPLYQLLRLVPGSFESVVAVGVPTALVFVLVALPFF
                     DRGSARNLLHRPLAAAALTAILGGSGLLLGAALRAGGPKIPPEVGRPLTSAERAGRAL
                     FASQQCNSCHKIMGKGGRDGPDLTEIGLHHSPAWLHSYIEAPARFHPDTKMAAFGPPT
                     LTHEEIEELAQYLSSLRGAAGPSVVPQYADTFPDAKPGGP"
     gene            2851..>3101
                     /locus_tag="VFU45_04375"
     CDS             2851..>3101
                     /locus_tag="VFU45_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358331.1"
                     /translation="MRIPSLDVLATLATATPGSAAAVGHDLAAVRAYKADYADGRAST
                     TVAGLIPAWARKYKMNCSGCHYPVPPRLNATGLRFRWAG"
BASE COUNT          406 a         1130 c         1086 g          479 t
ORIGIN      
        1 ctgcgttccc gggtccgcct ggcgcggctc gaacggggag cggacctgct gcgacggaag
       61 cgtgaagcgc tggtggcgga gctcttccgc ctggcccgcc ccgcgagcga tgcccggggg
      121 cgcatcgaac aggccgcaga ggaggcgtac gtggcgctgc tccccgccct gaccgaacac
      181 ggcgccgccg gcctgcgggc gctcggctgg ccggcgcggg actatcccgt ggaactggcg
      241 cccgggatgg tctggggcgt gccggcctcc cgcatcgtgt cgcggccgcc ggtgccacgc
      301 accctcggtg cccgtggcac cgcgcccgcc gccgtcggcg ggagcgcggt gcgggcggcc
      361 gatcggttcg agcgcctggt cgaggaactg ctcgaggcgg cgccgcggga gatgctcctc
      421 cgccggatcg gcgaggcgtt ggcgcgcacc tccaggcagg tcaatacgct cgagcggcgg
      481 ctggcgcctc gtctggcgcg cgatatcgtc cgggtccgcc aggtgctcga ggagcgggag
      541 cgggaggaac gggtgcggct ggacgggctc ctgcgccgcc ggcgttcccg ccccgccgca
      601 gaggcccgac cgtgagcggt ctttcctcga cccttcatcg cccgctcaca ctgcggacgt
      661 aggttcgggc atccagcgtg gcgggttcgc cacatctcgt tcgtgatctg acgcatctcc
      721 cttcgggagc ctgcatgacc gaggacccgg ctgccaacgg cctggcgacg cgacggcgat
      781 tcctcaagtg ggcgacggcc atcagctcgt tggcggccgc ggcgctcgcc ggggtgccgg
      841 tggtgcgggc gttcgtgtcc cccgccttcc gcaagccggt cgccaagcgc tgggtcaagc
      901 tgggcgaggc ggacctcttc gacgtgggcg cgcccacccg ggtggacttt gccgagacga
      961 tcgaggacgc gtgggtggag cagcggacgc tccgcagcgt ctgggtgtac agcgatgacg
     1021 gtgaggcgtt caccgtctac aacggccgct gcacccatct cggctgcgcg taccgcttcg
     1081 acaagaccgc cgatccgcag taccactccc aggagaacgt cttccactgc ccgtgccatc
     1141 acggcgtgtt cgacctgaag acgggtgcag tgctggcggg cccgccgccg cggccgctcg
     1201 acaccctcga gaccaagatc gaggacggaa tgctgtacgc cgcctacgag gacttccgcg
     1261 tgggcatcgc ggagcgggtg accacgtgaa gggcgtcctc cgctggctgg acgagcggat
     1321 cgacctggcg ggcctgaagc gcgccctgct cgaccgcgag gtcccggccc ggctcacctg
     1381 gtggcacacg ctcggcagcg ccaccctcac ggtattcctg ctgcagctgg tgaccggcat
     1441 cgtcctcgcc gcgtactact cgccctcgcc cgaccacgcc tacgacagcc tgcagttcat
     1501 cgggcgccag gtcagcagcg gggccctcct gcacggcatc caccactggg gcgccagtgc
     1561 catggtggtg ctcctcctgg cccacatgat ccgggtattc tcggtcgggg catacaagta
     1621 cccgcgcgag atcaactggc tggtcggcgt cggcctcttc ttcgtggtga tggccttcag
     1681 cttcacgggg tacctcctgc cctgggacca gaaggcgtac tgggccaccg ccgtcggcac
     1741 caacatcgcc ggcaccacgc ccggcatcgg cgggttcctg gtgagcctgc tgcgcggcgg
     1801 cggggaactc ggcagcgcca cgctgacgcg tttctactcc ttccacgtcc tctggctgcc
     1861 gctcttcctc ggcgccatgc tgttcgtgca cctggccatc ctggtgcgcc aggggatcgc
     1921 cccccggcca gccgcgctgg aagcgggcgc gccccgccgg accgacgcgg cggactaccc
     1981 cggctattac agggcggcat acgccgatac caagaaggcg gggatgcggt tctggcccga
     2041 cgtgatcggg aaggacgcga tggtggcgct cggcgtggtg gccctcatcg tcctgcttgc
     2101 ggccgcattc ggtgcgccgc tcgaggcgcc ggccgatccg accgacagcg cctatgtacc
     2161 tcgtcctgag tggtacttcc tccccctcta tcagctgctg cggctggtcc ccgggtcgtt
     2221 cgagagcgtg gtggcggtgg gcgtgccgac cgcgctcgtc ttcgtgctgg tggcgctgcc
     2281 gttcttcgat cgggggagcg cacggaacct cctccaccgg cccctcgcgg ccgccgcgct
     2341 caccgcgatc ctgggcgggt cggggctcct gctgggggcc gccctgcgcg ctggcggccc
     2401 gaagatccct cccgaggtgg ggcgacccct cacgtcggcc gaacgggcag ggcgggcgct
     2461 cttcgcgtcc cagcagtgca acagctgtca caagatcatg ggcaaggggg ggcgcgacgg
     2521 cccggacctc accgagatcg ggctccacca ctcgcccgcc tggttacaca gctacatcga
     2581 ggcccccgcc cgcttccatc ccgacacgaa gatggcggcg ttcggcccgc ccaccctcac
     2641 gcacgaagag atcgaggaac tcgcccagta cctgtcgtcg ctccggggcg cggcggggcc
     2701 atcggtggtg ccgcagtacg cggacacctt cccggacgcg aagcccggcg gaccctgatc
     2761 gaccggccct cggtccgcct cgcctcacgg aatcgtcgcg aacatcccgg tgtgcgccgg
     2821 taccatcact cagtgcctgg aggctgggat atgcgaatac cctctctcga cgtgctggcc
     2881 acgctggcca ccgccacgcc cggttcggcc gcggccgtcg ggcacgatct ggcggcggtc
     2941 cgcgcctaca aggcggacta cgccgatggt cgcgcatcca ccacggtggc cggcctgatc
     3001 ccggcgtggg cccggaagta caaaatgaac tgctccggct gccactaccc ggtgccgccc
     3061 cggctgaacg cgaccgggct gcgattcagg tgggcgggct a
//
LOCUS       DASUAP010000177         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_318742, whole genome shotgun sequence.
ACCESSION   DASUAP010000177 DASUAP010000000
VERSION     DASUAP010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318742"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFU45_04380"
     CDS             complement(<1..272)
                     /locus_tag="VFU45_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358332.1"
                     /translation="MRSQGKWCAFVLTAVQVAGCGGRNPPVTVVGPAADLRALSGSWW
                     GDYSSAITGRTGTIVFELQAGGDSAWGRVVMTPRGAVGPIMPWQNPR"
     gene            complement(292..1176)
                     /locus_tag="VFU45_04385"
     CDS             complement(292..1176)
                     /locus_tag="VFU45_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5358333.1"
                     /translation="MSWKPIVVGVDTSPAAARAGALGCEFAAFTHTSCHLIHAAETMA
                     SGDSARDIEALYARKRDEIQRTLWGYVPGHALERLSIRAGHPAVALRQAAAEVDAGLI
                     VLGGKHHTTVGEWAGRSTAMHLIRTAEIPVLIATSAQLPRRVLVALDQSAATGPTLEA
                     AERFAAQLGAQLKVLSVIEPLPTIGEVPPLDPEPYYQTSQSLIEKNVWPMITTPGVEK
                     IISYGLASSTIVREAKAWDADLVVMGSHGKGWFERVLVGSVTARVAHELPTSLLIVPV
                     YSALATGAEPSLTAAATV"
     gene            complement(1283..1717)
                     /locus_tag="VFU45_04390"
     CDS             complement(1283..1717)
                     /locus_tag="VFU45_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358334.1"
                     /translation="MPATYRLDLAERVVWSKAWGLLRDEELAAHSRALVTDPRFSPDF
                     RQVQDLLDVVDAPLTPSGLREVARLNPFGPGARRAVVVPSAVLFGLARMHENLREGAG
                     DELCVFRAMAPALEWLGLSPAWQPPPPASDDPLFQWGEPSGE"
     gene            complement(1761..1973)
                     /locus_tag="VFU45_04395"
     CDS             complement(1761..1973)
                     /locus_tag="VFU45_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358335.1"
                     /translation="MAISLFGAIFSGGLTYRELFQGAAKCPAPGAPGTVFGYPACVYG
                     FVMFTALLVTCSIALRAASRSGSGSQ"
     gene            complement(2000..3607)
                     /locus_tag="VFU45_04400"
     CDS             complement(2000..3607)
                     /locus_tag="VFU45_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5358336.1"
                     /translation="MSAGRRFLVAFVIAIVAIMLPDKCSGQGAVRRAVSQVNDSVTRA
                     QLSDLYGGWSWRLLWQGDSSATARAETLLAWLDRAGDDGLDPAAYDVAGLRASRSAEN
                     DSTRAAADIQFTLVYFRFGRDLAAGRVSPSLIDSLWDGSPAPPNLVAVLSRAIERDDL
                     NGALRSLRPPDFRYAALRGALLRYREIADRGGWPPVPDGPDLQQGDRGPRVAALRTRL
                     ELTEGLVSSPESAGVFGTDVTAALRRFQLRMGLTADGVAGPVTRAALNVPVDARITTI
                     EMNLERWRWAPRRLGSRYILVNIPAYLLELHDSGATVPFRAIVGRRDWPTPITDAWMD
                     GITFGPEWNVPRAIAVQEVVPLEAAHPGYLRDGGFRVLRVKTGAPVDPDSVDWRAVDT
                     AMFPYRLVQRSGPENPLGLIRFDVRDPFNVAVHDTPQRALFNDRVRIFSHGCVRLDRA
                     AVLAAHLLPEWSLDDVQSAMQGPPGRTVVLSRRVPVLLTYQTAWVESDGGVAFRDDVY
                     GWDDELSHALLRLAELPAAAALSGELR"
     gene            complement(3609..>4420)
                     /locus_tag="VFU45_04405"
     CDS             complement(3609..>4420)
                     /locus_tag="VFU45_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEU5358337.1"
                     /translation="LPSVAVKAPGFGDRRKAMLEDMAILTGGRVIAEEIGVKLEHVTL
                     ADLGHAKRVVVDREHTTIVGGAGAKEAIAGRCDDIRKQIADTTSDYDREKLQERLAKL
                     AGGVAVIRVGAPSETELKNRKEAFEDAISATKAAIAEGIVPGGGLALLRCIDPVERAA
                     AEAEGDERTGMLILRRVLEVPTRQIAENSALDGGVVVERMRAGTGNYGLDAAQGKYVD
                     LVDAGIIDPTKVVRVALENAVSVAGTLLLTEATLTEVPEPKAEVPVPAGMD"
BASE COUNT          742 a         1603 c         1415 g          660 t
ORIGIN      
        1 cgggggttct gccagggcat gatgggacct acggcccctc gcggcgtcat gaccacccgc
       61 ccccacgccg agtcgccacc agcctgtagc tcgaagacga tggtcccggt gcggccggtg
      121 atggcactcg aatagtcgcc ccaccagctc cccgacagcg ctcgaaggtc ggccgcgggg
      181 cccacgaccg ttaccggggg atttcttccg ccgcacccgg cgacctgtac cgccgtcagg
      241 acgaaggcac accacttccc ttgcgaccgc atacgtcctc cagaattgat gtcagaccgt
      301 ggcggccgcg gtcagtgaag gctcggcgcc cgtcgcgagt gccgagtaca cgggaacgat
      361 caggagcgac gtcggcagct cgtgcgcgac ccgcgccgtg acgctaccca ccagcactcg
      421 ctcgaaccaa cccttgccgt gcgatcccat caccaccagg tcggcgtccc acgcctttgc
      481 ttcccgcacg atggtcgaag acgccaaacc gtagctgatg attttctcca cgcccggtgt
      541 cgtgatcatc ggccagacgt tcttttcgat cagggactgc gacgtttggt agtacggctc
      601 cgggtcgagc ggcggcactt cgccgatggt gggcaggggc tcgatgacgc tcagtacctt
      661 caactgagcc ccgagctgtg cggcaaagcg ctcggcggcc tcgagggtcg ggccggtcgc
      721 cgcagactgg tcgagggcga cgagcacgcg ccgcggcagc tgcgccgagg ttgcgatcag
      781 cacggggatc tcggcggtcc ggatcaggtg catggctgtg ctccgcccgg cccactcgcc
      841 caccgtcgtg tggtgtttgc cgccaagcac gatgaggccg gcgtcgactt cggcggctgc
      901 ctggcggagc gccacggcgg ggtgccccgc acgaatcgac agccgctcca gcgcgtgccc
      961 aggcacgtat ccccagaggg tgcgctggat ctcgtcccgc ttgcgggcat agagggcttc
     1021 gatgtcacga gcggaatcgc ctgaggccat cgtctccgcg gcgtgaatca ggtgacaact
     1081 ggtgtgcgtg aaggcggcaa attcgcagcc gagcgctccg gcccgcgcgg ccgccggcga
     1141 ggtatcaaca ccgactacga tgggcttcca cgacatacga tccctccagt gcggggtctg
     1201 gcgggaaagg tggccggtgg tccatgagga actgatgaag gtcccatgca tcgtttatca
     1261 ggagcctcat gcgccttgag cgtcactctc cggacggttc cccccactgg aacagggggt
     1321 cgtcggaagc aggcggtggc ggttgccagg ccggtgagag ccccagccac tccagcgccg
     1381 gcgccatagc ccggaacaca cacagctcgt cccccgcacc ctcccggagg ttctcgtgca
     1441 tgcgcgcgag tccgaacagc accgctgacg ggacgacgac ggcccgacgc gccccaggac
     1501 cgaacggatt gagcctcgcc acctcccgaa ggccggaagg cgtgaggggg gcatccacca
     1561 catccagaag atcctgcacc tgacgaaagt ccggggagaa ccgcggatcg gtcaccagcg
     1621 cccgtgagtg cgcagcgagc tcctcatccc gcaggagccc ccacgctttg ctccatacca
     1681 cccgctcggc gaggtccaga cgataagtgg ccggcatcga tccgaaatct cgccgccgac
     1741 cacgaccctg cggaagcccg ctactgggac ccgctcccac tccgcgaagc ggcccgaagt
     1801 gcgatggagc acgtgaccag gagtgcggtg aacatcacga atccgtacac gcaggccgga
     1861 tacccgaaca ccgtgcccgg cgcgcccggc gcggggcatt tggccgcacc ctggaagagc
     1921 tcccgatagg tcagtccgcc ggagaagatc gcgccgaaca ggctgatggc caccgcccct
     1981 tgaatccatc gcttcgcatt catcgcagtt ccccgctcag ggcagccgct gccgggagct
     2041 ccgccaaccg cagcagggcg tggctcaact cgtcatccca gccgtacacg tcgtcccgga
     2101 aggccacgcc gccgtcactc tccacccacg ccgtctgata ggtcagcagc acgggtaccc
     2161 gccggctcag cacgacggtg cggcccgggg gtccctgcat cgcgctctgc acatcatcga
     2221 gagaccactc cggcagcaga tgggcggcga gtacagcggc gcgatcgagg cgcacgcagc
     2281 cgtggctgaa aatccggacc cggtcgttga acagcgcgcg ctgcggcgta tcgtggacgg
     2341 cgacgttgaa gggatcccgc acgtcaaacc gaatgaggcc gagcggattc tcaggcccgg
     2401 aacgctgtac cagccggtac gggaacatgg ccgtgtcgac cgctcgccag tcgacggaat
     2461 ccggatcgac gggcgcaccg gtcttgaccc gaagaacccg gaatccgcca tcccgcaggt
     2521 agcccgggtg cgccgcctca aggggcacga cctcctgcac cgcgatggcg cgcgggacat
     2581 tccattcggg accgaacgtg atcccatcca tccaggcatc ggtgatcggc gtcggccagt
     2641 cacgtcgccc gacgatggcc cggaatggga cggtcgcgcc gctgtcatgc aactccagca
     2701 ggtacgctgg gatgtttacc aggatgtagc gcgacccgag cctgcgcggg gcccaccgcc
     2761 accgctcgag gttcatctcg atcgtggtga tgcgggcatc gacggggacg ttcagggccg
     2821 cccgcgtgac gggccccgcg acgccgtcgg cggtcagccc catccgcagc tggaagcggc
     2881 ggagggcggc cgtcacgtcg gtgccgaaca caccggcact ttcgggtgac gacaccaggc
     2941 cctccgtcaa ctccaggcgc gttcgcagcg ccgccaccct cggtcctcga tccccctgct
     3001 gcaagtcggg accatcgggc acgggaggcc agcccccgcg gtccgcgatc tcccggtacc
     3061 gaagcagtgc gccacgcaag gcggcatacc ggaagtcagg gggacgcaga ctccggagag
     3121 ctccattcaa gtcgtcgcgt tcgatcgccc gactcaggac cgcgacgaga ttcggtggag
     3181 ccggacttcc gtcccagagc gagtcgatca gcgatggcga gacccgtccc gccgccaggt
     3241 cgcgcccgaa gcgaaagtag accagtgtga attggatgtc ggcggcggcc cgcgtggagt
     3301 cgttttccgc cgagcgactg gcgcggagtc ccgcgacgtc gtacgccgcc ggatcgagtc
     3361 cgtcgtcacc cgcccggtcg agccaggcga ggagcgtctc ggctcgcgcc gtcgcgctcg
     3421 aatcaccttg ccagagcagc cgccaggacc accccccgta caagtccgag agctgcgcgc
     3481 gggtcaccga gtcattcacc tgtgacaccg cccgccgcac cgccccctgc ccggagcact
     3541 tgtcgggcag catgatcgcc acgatcgcga tcacgaacgc aacgaggaag cgccggcccg
     3601 ctgacatgtc agtccatccc cgcggggacc gggacctccg ccttcggctc gggcacctcg
     3661 gtcagcgtcg cctccgtcag gaggagcgtg cccgccacgg agacggcatt ctcgagcgcg
     3721 acccgcacga ctttcgtcgg atcgatgatg cccgcatcca ccaggtcgac gtacttgcct
     3781 tgtgcggcgt cgaggccgta gttgccggtg cccgcccgca tccgttctac gaccactcca
     3841 ccgtccaagg cggagttctc ggcgatctgg cgggtgggga cctcaagcac ccggcgcaga
     3901 atcaacatgc cggtccgctc gtcgccctcg gcctccgcgg ccgcccgttc caccgggtcg
     3961 atgcaacgga gcagcgcaag ccctccaccc ggtacgatcc cttcggcgat ggccgctttg
     4021 gtcgcactga tcgcgtcctc gaaggcctcc ttccggttct tcagctccgt ctcggaggga
     4081 gcgcccaccc gaatgacggc gaccccgccc gcgagcttgg ccagccgttc ctggagcttc
     4141 tcgcgatcgt agtcggacgt ggtgtcggcg atctgcttcc ggatgtcgtc gcagcgaccg
     4201 gcgatcgcct ccttggcccc ggccccgccc acgatggtcg tatgctcccg atcgaccacg
     4261 acccgcttgg cgtgccccag atcggccagt gtcacatgct ccagcttcac accgatctcc
     4321 tcggcgatca cccgcccgcc cgtgagaatc gccatgtctt ccagcatcgc cttccggcgg
     4381 tccccgaatc ccggcgcctt caccgccacc gagggcagca
//
LOCUS       DASUAP010000178        14724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_141534, whole genome shotgun sequence.
ACCESSION   DASUAP010000178 DASUAP010000000
VERSION     DASUAP010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 14724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14724
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141534"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFU45_04410"
     CDS             complement(<1..685)
                     /locus_tag="VFU45_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358338.1"
                     /translation="MHSASWRLGEWTRPSSRLLAAGATLAALATPLCAQAYTAPMLAC
                     AAFHQSVDGRVRTELNGTTRLERAGRDGRLLVRAAAADSGLRTVVWWDSLRVWRDADA
                     GRIEPSAEGLLGGRYVGTLGAHGGWRGEKVPFIPDDVAAIADLTGVPDDLLPLLVPRP
                     LLVGATWNGGSELSVTRLTDSTANGHRWARFAIARKKLHQEERDIGGPALAKIEDSET
                     ETGRLTWDAS"
     gene            709..1968
                     /locus_tag="VFU45_04415"
     CDS             709..1968
                     /locus_tag="VFU45_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5358339.1"
                     /translation="MLLGAASLGAQAPAGEHRVLIHAGTLIDGVGDRPRSDQGILIAG
                     ERIVEVGPWASVAPHGAGADVIDLGHSTVLPGLIDTHTHVLLQGDVTAADYDEQLLKE
                     SIPYRAIRATAAARTALLDGFTTLRDLETEGAMFADVDVKTAIARGIVPGPRMFVSTR
                     AFAPTGMYPLLGYSWELDLPHGVQPIDGVDAIRQAVRDQVAHGADWIKFYADRRYYVA
                     ADGRLRSWVNFTDEEAKALVDEAHRLGHKVAAHAMGWDGIDAALRAGVNSIEHGYGLT
                     PDLMDRMVAQHVTWVPTIYIGVYVAPGRGGVWPRMADLERTAFGQALKRHVDIAFGTD
                     AGGFPWTENEAKEFGYMVRYGMTPMQAIQSATSRAAALLDAADDIGSVAPGRYADLVA
                     VDADPLADITALERVNWVMKGGVVYKK"
     gene            complement(2165..3793)
                     /gene="pruA"
                     /locus_tag="VFU45_04420"
     CDS             complement(2165..3793)
                     /gene="pruA"
                     /locus_tag="VFU45_04420"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEU5358340.1"
                     /translation="MSGIFSPPVPTNEPVLSYAPGSPERAALKAELARLSAIVTDIPV
                     IVGGKEVRGSKTVDVVMPHDHKHVIAKVHQADEAAVRDAITASLAARREWSEWSFAAR
                     AAVFLKAADLLAGPWRATVNAATMLGQSKTAFQAEIDSACELIDFLRFNVHFAERVYK
                     EQPMSAAQTWNRLDHRPLEGFIYAITPFNFTAIGGNLPSAPALMGNAVVWKPAATAAL
                     SNYFIYRIFEEAGLPAGVINFVPGSAGMVSDTLLNDEHLAGIHFTGSTEVFQTLWQSV
                     AKNLTTYRTYPRLVGETGGKDFVVAHRSADPAALAVALVRGAFEYQGQKCSAASRAYV
                     PAGLWPAVRDRMVAMLKEIKLGDVADFRNFMGAVIDRKAFDRIKGYLDEAKKSKGVTI
                     VFGGKCDDTTGYFVEPTLVQVEDPKYRMMCEEIFGPVLTVHVYPDAEWEQTLELVNGT
                     SPYALTGAVFATDRGALATADRVLRNAAGNFYINDKPTGAVVGQQPFGGARASGTNDK
                     AGSILNLLRWVSPRTVKENFVPPVDYKYPFMREQ"
     gene            complement(3852..4751)
                     /locus_tag="VFU45_04425"
     CDS             complement(3852..4751)
                     /locus_tag="VFU45_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5358341.1"
                     /translation="MFCAAAPLRLSAQSLSVYTAGSLARPFRALLDSFRVAYPGLVPD
                     QVNAGSVDLARRIVDLGQVPDVYATADIDVIPRLLVPRAADWYLAFARNEMVLLHGPS
                     SAGADSVDGTNWWRVVTAPGVRMGSSDPALDPSGYRTLLVFELAERHYRVPGLAARLR
                     AALPLRYIRPSETDLIALVEAGELDYAWSYRSLAVAAGLPYVALPPEIDLGDPADSAL
                     YAAASVRVERGTGTAPLEVRGSPILYGLTIPRSAPHRELARAFVMYVLSPAGRAILAR
                     NGLTPLERPRRFGRPPGNLPMFQ"
     gene            complement(4838..5212)
                     /locus_tag="VFU45_04430"
     CDS             complement(4838..5212)
                     /locus_tag="VFU45_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5358342.1"
                     /translation="MAASRVLIVDDDKTMVAVMTAVLKAGGFQVSAAFDAAQGFMFAQ
                     KVPPDLILLDMNMPAGGGAGTWQRLRQSARTQSVPVVFVTAESKPGLEQEVLRQGAVG
                     FIQKPFDPATLADRVRGFLERK"
     gene            complement(5215..6015)
                     /locus_tag="VFU45_04435"
     CDS             complement(5215..6015)
                     /locus_tag="VFU45_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5358343.1"
                     /translation="MPTVFRRGSVALLALLSLAPTATSAQAPAALPVASDSNPRLPGA
                     PIRWWHVAVATGGVFLASAADNAFHDLAQNHNTQTDRDVATAIQHFGSGVVPVAIPAA
                     IIGVGLISRNSDLTRSGLRVGGSLAVGAVITEVLKEAVGRARPYQGPAVTGFKPVSGR
                     ASMPSGHTMVAVALATSLSDEIHRGWATAGLYTLAAGTGASRIVLNAHWFSDVVAGAI
                     IGTTSAKLVNGKWRLFRITPPRILIGPDRLGLQWSLGPLRAPSAGLQP"
     gene            6125..6490
                     /locus_tag="VFU45_04440"
     CDS             6125..6490
                     /locus_tag="VFU45_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358344.1"
                     /translation="MRRYLLAVAAAAALAGCRTYDSYDPLASQAGLLPADRYAAFGRE
                     QAEAIAIGRELGGQHGLSEAEQYKSVVAYAKAQPDVVSVIADSQAHILTVQFKSGWVV
                     GVDPINDGKHGAETAIPGK"
     gene            6575..6898
                     /gene="cutA"
                     /locus_tag="VFU45_04445"
     CDS             6575..6898
                     /gene="cutA"
                     /locus_tag="VFU45_04445"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEU5358345.1"
                     /translation="MAIVIVVSTSLPSREAADRLARTLVDERLAACVQVSGPVRSTYR
                     WRGALEITDEWRCEIKTTDDAWPRARDRIIALHSYEVPEILMTRAEASAAYAEWVGQQ
                     VGSEK"
     gene            7117..7854
                     /locus_tag="VFU45_04450"
     CDS             7117..7854
                     /locus_tag="VFU45_04450"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEU5358346.1"
                     /translation="MTAIPTPPPLSAEPPATWEDLLEIFYAPSAVFERRKAAPRFWLA
                     FAVVSILVAVILFAGKGLLQPAFDAEFARGMAQAMKNNPQLTPDQLEKGRSFGTAFAT
                     IAMILGAPVAMLLLGPVIWLCGKLVGAVEDINAAWMIAALAYVPKIVGSVLLVIQPLV
                     MPPEAVKGFASLAIGPARFLDPDTSSKLLMLVATRLDLFTIWSTVIIVVGLKVTGKVP
                     AAKAWTAAAVVWLIPALFGLIGVLRAQ"
     gene            7882..8154
                     /locus_tag="VFU45_04455"
     CDS             7882..8154
                     /locus_tag="VFU45_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358347.1"
                     /translation="MKRSVVITIVVVAAFAGLLLYSTLSAQRVQCDVCVSFNGLSNCA
                     SATAANEMEAARSAQSTACGVLARGMAESINCGDAQPTKRVCQHAA"
     gene            8354..8785
                     /locus_tag="VFU45_04460"
     CDS             8354..8785
                     /locus_tag="VFU45_04460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5358348.1"
                     /translation="MGELIIRDMEQGEEFVAWFAELVDRQDESTGAELSYHDRYLILT
                     NEVGDWIGGVRFYLRGGVAHLLEFAVEADHEDLGYGLRLLEAFEQRARDADAHLAEFW
                     ANDFESTGVYGALGWREVVRRERYVGRGPWVLMEKRLDATP"
     gene            8848..9822
                     /locus_tag="VFU45_04465"
     CDS             8848..9822
                     /locus_tag="VFU45_04465"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEU5358349.1"
                     /translation="MTLTDAARAFARKIEVLRDLETKVLEMMRAHEAKRPLWYPQDLL
                     APAPDSCPDTHTADLRQRAEGIPDAARAALAVNLLTEEGLPHFHRLLAVYLGDDSHWQ
                     QWNFLWTAEEDRHGAILHDYIRDTRLVDQRKLEELQFNYIRNGFTPAWDRDPYRVFVY
                     TTVQERATQLSHANTGKLCGEYEPVIGEVLANVASEEARHFTFYRNVFTEILARDPDE
                     ALHSASFILPSIDMPGHTIPGFRELADVIRRAGIYGPRDYLRIVQEQIRHWKIEHLSG
                     LSEMGRIAQEKILQIPERLRRISELIETRSRAKTFAFDVVFRREFAME"
     gene            9825..10286
                     /locus_tag="VFU45_04470"
     CDS             9825..10286
                     /locus_tag="VFU45_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358350.1"
                     /translation="MRASWALLSVALAAACGHKAGTSAAAAPAADSAASVPPAATPAQ
                     VTQEATQLGREVFELVDKTMGYYSAHFGEFPRNVNAIGVDSLTPTTVRRLAVHGKIPT
                     VTAAFRRPASHAVTACSATNKVLEDSMLNGGPYTVDCTLADGSVRSITVGG"
     gene            10305..10892
                     /locus_tag="VFU45_04475"
     CDS             10305..10892
                     /locus_tag="VFU45_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU5358351.1"
                     /translation="MTLSLFFSVLRGAVLAVNLFALTVALIAWGVRTRRISPFNRWAG
                     MVRRWGDPLVRATERRLLASGGDPTTAPWWLLGTTVVGGLALLSLAQWAVAAGFTVEA
                     AAESGPVGLLALAVDLAYDVVVLALLVRVVGSWFGIGRWTRWMRPAYQATDWIVGPLS
                     RAIPPLGVVDVSPLAAWLLLWMVRGLVVHVILRAG"
     gene            11003..11311
                     /locus_tag="VFU45_04480"
     CDS             11003..11311
                     /locus_tag="VFU45_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEU5358352.1"
                     /translation="MFPITAASSGVLLRLHVQPRASRTEVIGLHGDAVKVRLAAPPVD
                     GAANDELVRFLARTLGVPSGAVTIVAGASGRRKQVMVAGIDVAHSMSRLLGSGTSDSG
                     "
     regulatory      11391..11465
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            11670..>14724
                     /gene="metH"
                     /locus_tag="VFU45_04485"
     CDS             11670..>14724
                     /gene="metH"
                     /locus_tag="VFU45_04485"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEU5358353.1"
                     /translation="MTSRRADHLFRVIRERILVLDGAMGTMLQRLGLTEGDFRGTRFR
                     THPTDLRGNNDLLTLTRPDAVRDVHAAYLAAGADILETNTFNSTSISQADYGLQAIVR
                     ELNEAGARLAREAADAAESRDPSRPRFVAGVLGPTSRTASLSPDVNDPGTRQVDFDQL
                     VAAYREAAAGLLDGGSDLLLVETVFDTLNCKAALFAVEEEFAARGHRVPVMVSVTITD
                     RSGRTLSGQAPEAFWTSIAHARPFSVGLNCALGPRELAPHLRELSRVSPAFVSIHPNA
                     GLPNAFGGYDETPAAMASVIGDWAAQGLVNIVGGCCGTTPEHIAAIADAVRGRAPRRP
                     PAIEPRLRLAGLEPFTLGPDTLFVNIGERANVTGSRRFARLIEAGDYAAGLEVARQQV
                     ASGAQMLDLNFDEAMLDSPRAMERFLRLLAAEPDISRVPVVLDSSKWSVIEAGLKNVQ
                     GKPVVNSISLKEGESEFLRQARLARRYGAAVIVMAFDERGQADTVERRVEVLTRACRL
                     LVDEAGMPPEDVIVDPNIFAIGTGLPEHARYAVDYLEATRRIKAALPAVHVSGGLSNL
                     SFAFRGNDVIREAIHSVFLFHAIQAGMDLGIVNAGALPIYEDIPPDLRERVEDLVLDR
                     RPDATERLLAVADAVKGRAAAATVDAEWRTLPVHERLAHALVHGINQFIVADTEEARQ
                     GASHPVEVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLVPFLEAEKAR
                     NPGTRPAGRVVLATVKGDVHDIGKNIVGVVLQCNNYEVTDLGVMTPSDRILDAARESG
                     ADIIGLSGLITPSLGEMSFVASEMEREGFRIPLLIGGATTSRIHTAARIAPKYSGPVV
                     HVADASRAVAVASTLLNPAERGAFAERTAAEYRGLAREHAERGSAARPTIAEARARRP
                     TVDTSRPAPRPSFLGVRSLAPWPLPELVERIDWTPFFQTWELKGRYPDILRDPSVGET
                     ARSLHADALALLDRIVREGLLEARAVYGFFLAERAGDDDIALLSPD"
BASE COUNT         2313 a         5247 c         5040 g         2124 t
ORIGIN      
        1 tcgacgcgtc ccaggtgagc cggccggtct cggtctcgct gtcctcgatc ttcgcgagcg
       61 ccggcccccc gatgtcgcgc tcctcctgat ggagcttctt ccgggcgatc gcgaaccggg
      121 cccagcgatg tccgttcgcg gtgctgtcgg tgaggcgggt gacggagagc tcggagccgc
      181 cgttccacgt ggccccgacc aggagcgggc gcggcaccag gagggggagg aggtcgtccg
      241 gcaccccggt caggtcggcg atggcggcca cgtcgtcagg aatgaacggc accttctcgc
      301 cgcgccagcc gccatgagcg cccagcgtcc ccacgtagcg cccgccgagg agcccttcgg
      361 ccgacggctc gatacggccg gcgtcggcat cgcgccagac gcggagcgaa tcccaccaca
      421 ccaccgtgcg gaggccgctg tcggccgccg cggcgcggac caggagccgg ccgtcgcgcc
      481 ccgcgcgctc gagccgcgtg gtgccattga gttcggtccg cacccggcca tcgaccgact
      541 ggtggaaggc cgcgcacgcg agcatcggcg ccgtgtaggc ctgcgcgcag agcggggtgg
      601 cgagcgccgc cagggttgcg cccgcggcga gcaggcgcga agatgggcgg gtccactcac
      661 cgaggcgcca cgatgcggaa tgcatgcctg aagttagccg cgggagccgt gctcctggga
      721 gccgcctcgc tcggcgccca ggcccccgca ggggaacacc gggtgctgat ccacgccggc
      781 acgctgatcg acggcgtggg tgaccgcccg cgcagcgatc aggggatcct catcgccggc
      841 gaacggatcg tggaggtcgg cccctgggcg agcgtggccc cgcacggcgc cggcgccgac
      901 gtgatcgacc tcggccactc gaccgtactg ccggggctca tcgacaccca cacccatgtc
      961 ctgctccagg gcgatgtgac cgccgccgac tacgacgagc agctgctcaa ggagtcgatc
     1021 ccgtaccggg ccatccgcgc cactgcggcc gcgcgcacgg cactcctcga cgggttcacg
     1081 acgctccgcg acctcgagac cgaaggggcg atgttcgccg acgtcgacgt caagaccgcc
     1141 atcgcgcggg ggatcgtccc cggcccgcgg atgttcgtct cgacccgcgc ctttgccccg
     1201 acggggatgt atcccctcct cggctactcc tgggagctcg acctccccca cggcgtccag
     1261 cccatcgacg gtgtcgacgc gatccggcag gcggtgcgcg accaggtggc ccacggcgcc
     1321 gactggatca agttctacgc cgaccgccgg tactacgtgg ccgccgacgg gcggctccgc
     1381 agctgggtca acttcaccga tgaggaggcg aaggcgctgg tggacgaggc gcaccggctg
     1441 gggcataagg tcgcggccca tgcgatgggg tgggacggga tcgacgccgc gctccgggcc
     1501 ggggtcaact cgatcgagca cggctacggg ctcacccccg acctgatgga ccggatggtc
     1561 gcccagcacg tgacatgggt ccccacgatc tatatcgggg tctacgtcgc gccgggccgc
     1621 ggcggcgtgt ggcccaggat ggcggacctc gagcgcacgg ccttcggcca ggcgctcaag
     1681 cgccacgtcg acatcgcgtt cggcaccgac gccggcggct tcccctggac cgagaacgaa
     1741 gcgaaggagt tcgggtacat ggtgcgctat gggatgacgc cgatgcaggc catccagtcg
     1801 gccacgagcc gcgccgcagc gctgctggac gccgcggacg acatcggcag cgtggcaccg
     1861 ggtcgttacg ccgacctggt cgcggtcgac gccgacccgc tcgccgacat cacggcgctg
     1921 gaacgggtga actgggtgat gaaaggcggg gtggtctaca agaagtgaaa agggaagagg
     1981 gaaaaggaga aggagaagga gaagggggaa ggagaaggca gaaggagaag ggagaaggag
     2041 aaggctgaag gagaaggaag aggagaaaga ggaggaagaa gggaaaaggg aaaagggaaa
     2101 aggggctccg atccacccgg cgcccctgtt cccttttcac ttttcacttt tccctttccc
     2161 ctcttcactg ctcccgcatg aacggatact tgtagtccac cggcggcacg aagttctcct
     2221 tcaccgtccg cggcgagacc cagcgcagca ggttgaggat cgagccggcc ttgtcgttgg
     2281 tgccgctggc acgggcgccg ccgaagggct gctgccccac cacggccccg gtcggcttgt
     2341 cgttgatgta gaagttgccg gccgcgttcc ggagcacccg gtccgcagtg gcgagcgcac
     2401 cccggtccgt ggcgaacacc gccccggtca gcgcgtacgg gctcgtcccg ttcaccagct
     2461 cgagcgtctg ctcccattcg gcgtccggat agacgtgcac cgtgagcacg gggccgaaga
     2521 tctcctcgca catcatccgg tacttcgggt cctcgacctg caccagcgtg ggctcgacga
     2581 agtagccggt cgtgtcgtca cacttcccgc cgaagacgat ggtcacgccc ttcgacttct
     2641 tcgcctcgtc gagatagccc ttgatccggt cgaacgcctt ccggtcgatc acggcgccca
     2701 tgaagttccg gaagtcggcg acgtcgccca gcttgatctc cttgagcatc gcgaccatcc
     2761 ggtcgcgcac ggcgggccag agcccggccg gcacgtaggc gcgggaagcg gcgctgcact
     2821 tctgcccctg gtactcgaag gcgccgcgca ccagcgccac ggcgagcgcc gcgggatcgg
     2881 cgctccggtg cgccacgacg aagtccttgc cgccggtctc gccgacgagg cgggggtacg
     2941 tccggtacgt cgtgaggttc ttcgcgacgg actgccagag ggtctggaac acctcggtcg
     3001 agcccgtgaa atggatcccc gccaggtgct cgtcgttgag gagcgtgtcg gagaccatcc
     3061 cggcgctccc ggggacgaag ttgatgacgc ccgcggggag tccggcctcc tcgaagatcc
     3121 ggtagatgaa gtagttgctg agcgccgcgg tggccgccgg cttccacacc acggcgttcc
     3181 ccatcagcgc cggggccgag gggaggttgc cgccgatggc ggtgaagttg aacggcgtga
     3241 tggcgtagat gaacccctcg agcggccggt ggtcgagccg gttccacgtc tgggccgcgc
     3301 tcatcggctg ttccttgtac acccgctcgg cgaagtgcac gttgaagcgc aggaagtcga
     3361 tcagttcgca ggccgagtcg atctccgcct ggaaggccgt cttgctctgg cccagcatcg
     3421 tcgcggcgtt gaccgtggcg cgccagggcc cggcgagcag gtcggccgcc ttgaggaaca
     3481 ccgccgcgcg cgccgcgaag ctccactcgc tccattcgcg acgggcggca aggctcgccg
     3541 tgatcgcatc gcgcacggcg gcctcgtcgg cctggtgcac cttcgcgatc acgtgcttgt
     3601 ggtcgtgcgg catcaccacg tccacggtct tcgacccacg gacctccttc ccgccgacga
     3661 tcacggggat gtcggtcacg atggcagaga gccgggccag ctcggccttg agcgcggcac
     3721 gctccgggga gccgggggcg tacgagagga ccggttcgtt ggtcgggaca ggcggggaga
     3781 agatgccaga catatctaga ctcagggaag aaggacgctc agacgctcga tccgaagata
     3841 atgacccgca tctactggaa catcggcagg ttcccgggcg ggcgcccgaa tcggcgcgga
     3901 cgttcgagcg gggtcaatcc attccgcgcc aggatggccc gcccggcggg cgagagcacg
     3961 tacatcacga aggcccgggc gagctcgcgg tgcggcgccg agcgcgggat ggtcaggcca
     4021 tagaggatcg gcgacccacg gacctcgagc ggagcggtac ccgttccccg ctcgacccgc
     4081 accgaagcgg ccgcgtacag ggcgctgtcg gcgggatcgc ccaggtcgat ctcgggcggc
     4141 agcgccacgt acggcaggcc cgcggcgacg gccagggaac ggtacgacca ggcgtagtcg
     4201 agctccccgg cctccaccag cgcgatgaga tcggtctcgc tcggccggat atagcgcagg
     4261 ggcagcgcgg cgcgcagccg cgccgccagc ccggggaccc ggtagtggcg ctcggccagc
     4321 tcgaacacca gcaacgtgcg gtatccgctc gggtcgagcg ccgggtccga tgagcccatc
     4381 ctgacgccgg gcgcggtgac gacccgccac cagttcgtgc cgtcgaccga atcggcgccg
     4441 gcggacgagg gtccgtgcag gagcaccatc tcgttccgcg cgaaggccag gtaccagtcc
     4501 gccgcacggg gaacgagcag ccgggggatc acgtcgatgt cggcggtcgc gtacacgtcg
     4561 ggcacctgcc ccaggtcgac gatgcggcgg gccaggtcga cgctgccggc gttcacctgg
     4621 tccgggacga ggccgggata tgccacccgg aagctgtcga ggagggcacg gaacgggcgc
     4681 gcgagggagc cggcggtgta gacggagagg gactgagcgg agaggcgcag tggtgcagcg
     4741 gcgcagaaca acaagaggga cagaactgcg accggccggc cgccgcacca tcccgatccc
     4801 aggtccctgc gctgctgcgc ctccgcgccc ctccgcctca cttccgctcc aggaatcccc
     4861 gcacccgatc ggcgagcgtg gccgggtcga agggcttctg gatgaacccc accgcgccct
     4921 ggcgcagcac ttcctgctca aggcccggct tcgactccgc cgtcacgaac acgaccggga
     4981 cactctgggt tcgggccgac tgccggagcc gctgccacgt gccggccccg ccgcccgcgg
     5041 gcatgttcat gtcgagcagg atgaggtccg gcgggacctt ctgggcgaac atgaacccct
     5101 gggcggcatc gaacgccgcg ctgacctgga agcccccggc cttgaggacc gcggtcatca
     5161 cggcgaccat cgtcttgtcg tcatcgacga tgaggacgcg ggaggcggcc attcctaggg
     5221 ctgcaggccg gcggaaggag cccgcagggg gccaaggctc cactgcagcc ccagccggtc
     5281 cgggccgatg aggatccgtg gcggcgtgat gcggaagagg cgccacttgc cgttcaccag
     5341 cttggccgag gtcgtgccga tgatggcgcc ggccaccacg tcgctgaacc agtgggcgtt
     5401 gagcacgatg cgcgaggcgc cggtccccgc ggcgagggtg tagagtccgg cggtggccca
     5461 gccgcggtgg atctcgtcgg agagcgacgt cgccagggcg accgcaacca tcgtgtgccc
     5521 cgacggcatc gacgcccgtc cggagaccgg cttgaacccg gtcacggcgg ggccctggta
     5581 cgggcgcgcc cgaccgaccg cttccttgag cacctcagtg atcacggcgc cgacggccag
     5641 actccccccg acccgcagcc ccgagcgggt caggtcggaa ttgcgggaga tgagccccac
     5701 gccgatgatc gccgccggga tcgccaccgg gacgaccccc gagccgaagt gctggatggc
     5761 cgtggcgacg tcccggtcgg tctgggtgtt gtggttctgg gccaggtcgt ggaaggcgtt
     5821 gtcggcggcg gacgcgagga acacgccccc cgtggcgacg gccacgtgcc accagcggat
     5881 cggcgcaccg ggaagccgtg gattggaatc actcgccacc ggcagggcgg cgggtgcctg
     5941 ggcggaggtg gcggtcggcg ccagggagag cagcgcaagg agggccacgg agccacggcg
     6001 gaagacggta ggcatggtca gaaaatagcc ccgggggccg caaaatccgc catatcaatt
     6061 agattaaggc gctcccaata tttccggacg ccgtgcgcgc gtccccagat ccggatccga
     6121 cctcatgcgc cgatacctcc ttgccgtcgc cgcagccgcc gcccttgccg ggtgccgcac
     6181 gtacgactcg tacgaccccc tcgcctccca ggcgggcctc ctcccggccg accgttatgc
     6241 cgccttcggc cgggaacagg ccgaggcgat cgcgatcggc cgcgagctcg gcgggcagca
     6301 cggcctgagc gaggccgagc agtacaagtc cgtcgtggcg tacgccaagg cgcagcccga
     6361 tgtcgtgagc gtgatcgcgg acagccaggc ccacatcctg accgtccagt tcaagagcgg
     6421 gtgggtggtc ggggtcgacc cgatcaacga cgggaagcac ggcgccgaga ccgcgatccc
     6481 cggcaagtag gggcgcgcct gcgcgacccg aaaagacgcg tgccgagtgg cgggtgggtg
     6541 gcggggagca taggacccag agaactggcg ctacgtggcg atcgtgatcg tcgtctccac
     6601 ctcgcttccc tcccgggaag ccgccgaccg cctggcccgc accctggtcg atgagcggct
     6661 cgccgcctgc gtccaggtct ccggcccggt tcggagcacg tatcgatggc ggggcgcgct
     6721 cgagatcacc gacgaatggc gctgtgagat caagaccacc gacgatgcgt ggccccgggc
     6781 ccgcgaccgg atcatcgcgc tccactcgta cgaagtcccc gagatcctga tgacgcgggc
     6841 cgaggcgagc gcagcgtatg cggagtgggt gggacagcag gtaggaagtg agaagtgaga
     6901 agtgaaacgg aaaaggtgaa gagggaaaag ggagaagggt aagaagaaga cgtgaagagg
     6961 tgacgggtga tgcggcagcc cggatgacac accatcctga gcatagcgaa ggaccggcgg
     7021 gctgcctgac cgacacttcg gtcgttccac tccctcagga tgacgtcgct cgccagacga
     7081 tcggatgacg aacagccccc tggacccgga cctgtcatga ccgccatccc tacgccgccg
     7141 cccctctccg ccgagccgcc cgccacctgg gaggacctcc tcgagatctt ctacgcgccg
     7201 agcgccgtgt tcgagcggcg aaaggcggcg ccgaggttct ggctggcgtt cgcggtcgtg
     7261 tccatcctgg tcgccgtgat cctctttgcc gggaagggac tcctgcagcc ggcattcgac
     7321 gcggagttcg cgcgcgggat ggcgcaggcg atgaagaaca acccccagct cacccccgac
     7381 cagcttgaga aggggcggag cttcgggacc gccttcgcca ccatcgccat gatcctcggc
     7441 gccccggtgg ccatgctcct cctggggccg gtcatctggc tctgcggcaa gctggtcggc
     7501 gccgtcgagg acatcaatgc cgcatggatg atcgcggcgc tcgcctacgt gccgaagatc
     7561 gtcggctcgg tgctcctggt catccagccc ctggtgatgc cgccggaggc agtgaaggga
     7621 ttcgcatcgc tggccatcgg cccggcgcga ttcctcgacc ccgacacctc gagcaagctc
     7681 ctgatgctcg tggccactcg gctcgacctc ttcaccatct ggagcaccgt catcatcgtg
     7741 gtcgggctca aggtgaccgg caaggtcccg gccgcgaagg cgtggaccgc cgccgccgtg
     7801 gtctggctca ttccggcgct ctttgggttg atcggcgtgc tccgggcgca gtagcgccct
     7861 tccctacctc gcgagcgaac catgaagcgc agcgtggtca tcaccatcgt cgttgtcgcc
     7921 gccttcgcgg ggctcctgct ctactccacg ctctccgccc agcgggtcca gtgcgacgtg
     7981 tgcgtctcct tcaacgggct ctccaactgc gcgtcggcca ccgcggcgaa cgagatggag
     8041 gcggcccgga gcgcgcagag caccgcatgc ggcgtcctgg cgcgcgggat ggcggaatcg
     8101 atcaactgcg gcgacgcgca acccaccaag cgggtctgcc agcacgccgc ctgattcacc
     8161 cgcgttcggc cacgtgacac gggtcactcc gcgccttccg cggtctcccc gcatatcggc
     8221 gccaagtcgt tcgccgacaa ccgtgtagcg caccttcatc ccgacgcccg ccgaggctgg
     8281 caccatgctt gcggcgggat gctcgcgtcg cgcgtccggt ccccccgccc gggcgcccgc
     8341 atcggaggcc cccttggggg aactgatcat ccgggacatg gaacagggcg aggagttcgt
     8401 cgcctggttc gccgagctgg tcgaccgcca ggacgaatcg accggcgcag agctgtcata
     8461 tcacgatcgc tacctcatcc tcaccaatga agtaggcgac tggatcggcg gcgtgcgatt
     8521 ctacctccgc ggcggggtgg cgcacctcct cgagttcgcg gtcgaggccg accacgagga
     8581 cctgggctac ggcctccggc tcctcgaggc gttcgagcag cgcgcccgcg acgccgacgc
     8641 ccatctggcc gagttctggg ccaacgactt cgagtccacc ggggtctacg gtgcgctcgg
     8701 ctggcgggag gtggtccgac gggagcggta cgtggggcgc ggcccctggg tcctgatgga
     8761 aaagcggctg gacgccacgc cctgaagctt tggacataaa cccgcccggg gctatcgaaa
     8821 tggcgtccga acggccactt ttcggccatg acgctgactg acgctgcccg cgcttttgcc
     8881 cgcaagatcg aggtcctgcg cgaccttgag acgaaggtcc tcgagatgat gagggcccac
     8941 gaggccaagc gcccgctctg gtatccgcag gatctcctcg cgcccgcgcc cgacagctgc
     9001 cccgatacgc acacggcgga cctccgccag cgggccgagg gcatccccga tgcggcgcgg
     9061 gcggcgctcg cggtcaacct cctcaccgag gagggcctgc cccacttcca ccgcctcctc
     9121 gcagtgtacc tgggcgatga cagccactgg cagcagtgga acttcctctg gaccgccgag
     9181 gaggaccgcc acggcgccat cctccacgat tacatccgcg acacgcggct ggtggaccag
     9241 cggaagctcg aggagctgca gttcaactac atccggaacg ggttcacccc ggcctgggac
     9301 cgggacccgt accgcgtatt cgtctacacc acggtgcagg agcgggccac gcagctgagc
     9361 cacgccaaca ccgggaagct ctgcggcgag tacgagccgg tcatcggcga ggtgctggcc
     9421 aacgtggcga gcgaggaggc ccggcacttc accttctacc ggaacgtctt caccgagatc
     9481 ctggcccgcg accccgacga ggcgctccac tcggcgtcgt tcatcctccc gagcatcgac
     9541 atgccggggc acaccatccc cgggttccgc gagctggccg acgtgatccg ccgcgccggg
     9601 atctacggcc cacgcgatta cctgcgcatc gtgcaggagc agatccggca ctggaagatc
     9661 gagcacctga gcggcctgag cgagatgggc cgcatcgcgc aggagaagat cctccagatc
     9721 cccgagcggc tgcgccgcat ctcggagctc atcgagaccc gcagccgggc caagaccttc
     9781 gccttcgacg tggtgttccg acgcgaattc gccatggagt gacgatgaga gcctcctggg
     9841 ccctgttgtc ggtcgccctc gccgcggcgt gcggacacaa ggccggcacc tcggccgccg
     9901 ccgcaccggc agccgattcc gcggcatccg ttcctcccgc ggcgaccccg gcccaggtca
     9961 cccaggaagc cacccagctc ggccgcgaag tgttcgaact ggtcgacaag acgatgggct
    10021 actactcggc gcacttcggc gagttccccc gcaacgtgaa tgccatcggc gtcgacagcc
    10081 tgaccccgac gaccgtccgc cgcctggccg tccacggcaa gatccccacc gtcaccgccg
    10141 ccttccggcg ccccgccagc cacgccgtca ccgcgtgcag cgcgaccaac aaggtgctcg
    10201 aggactcgat gctcaacggc ggtccgtaca ccgtcgattg cacgctggcc gacggcagcg
    10261 tccgttccat caccgtcggc ggctgacgtt cccactcgcg cccggtgacg ctctccctgt
    10321 tcttctcggt cctccgcggc gccgtcctcg ccgtcaacct gttcgcgctc acggtcgcgc
    10381 tcatcgcgtg gggcgtccgc acgcgccgga tctccccctt caaccgctgg gccgggatgg
    10441 tgcgccgctg gggcgatccc ctggtccgcg ccaccgaacg ccggctcctt gcctccggcg
    10501 gcgacccgac cacggcgccg tggtggctcc tcggcaccac ggtggtcgga ggcctcgccc
    10561 tcctcagcct ggcgcaatgg gccgtggccg ccggcttcac cgtcgaggcc gcggcagagt
    10621 cggggccggt cggcctcctc gcgctggcgg tcgacctggc ctacgacgtg gtggtcctgg
    10681 ccctgctggt ccgggtcgtc ggctcgtggt tcggcatcgg gcgctggacc cgctggatgc
    10741 gccccgcgta ccaggcgacc gactggatcg tgggcccgct ctcccgcgcc atccccccgc
    10801 tcggcgtggt cgacgtgagc ccactcgcgg cgtggctgct gctgtggatg gtacgcggac
    10861 tcgtcgtcca cgtgatcctg cgagcggggt aagagggcgg ccagcagctg gcggctgtca
    10921 cgtctcaggt cgtaagtcgc aggtctcaga tcttgcgtcg cacatcttac gtctcacgtc
    10981 tcacgaccca cgactcacga ccatgtttcc gatcaccgct gcatcgagcg gcgtcctcct
    11041 gcgactgcac gtccagccga gagcctcgcg taccgaggtc atcgggctcc atggcgatgc
    11101 agtcaaggtc cggctcgcgg ccccgccggt cgacggtgcg gcgaatgacg agctcgtccg
    11161 cttcctggcg aggaccctcg gcgtgccgag cggcgccgtg acgatcgtgg ccggcgcctc
    11221 cggccgccgc aagcaggtga tggtcgcagg catcgacgtg gcacactcga tgagtcgcct
    11281 gctgggcagc ggaacatcgg atagcggata gctgatcgct gatcgctgat cgctcgcagc
    11341 acccgccccc tacccccgcc gtacggcacc cccatatatt ccgccagaca attgaaactc
    11401 gtggcgattt gcgcgcgctt gcggccacgt taatcaacag ctaaaacgcc cccgaacccc
    11461 ggtgccgttt tggcgccggg gcgtggcgtt ttgggggtag cgagtggcaa gtggcgggtg
    11521 gaaggtgagc gacggggagc gggagccggg gcgccggagg gaccgcacca cggcaaggtg
    11581 catggcatcg ccggcaacga ccgcctgaga gctgacacct gataactgat gaccgacagc
    11641 tggttccaac gaccatcgac caacgaccaa tgacctcccg ccgcgccgac catctcttcc
    11701 gcgtcatccg ggagcgcatc ctcgtgctcg atggggcgat ggggacgatg ctgcagcggc
    11761 tgggactcac cgagggagac ttccgcggaa cgcgcttccg cacccatccg acggatctca
    11821 ggggcaacaa cgacctcctc accctgaccc gcccggacgc ggtaagggac gtgcacgccg
    11881 cctacctcgc ggccggcgcc gacatcctcg agaccaacac cttcaattcg accagcatct
    11941 cccaggccga ctacggcctg caggccatcg tccgcgaact caacgaggcg ggcgcccggc
    12001 tcgcgcgcga ggcggcggac gcggcggagt cgcgcgatcc ctcccggccc cgcttcgtcg
    12061 ccggcgtgct cggcccgacc agccgcactg cgtcgctctc ccccgacgtc aacgaccccg
    12121 gcacccggca ggtggacttc gaccagctcg tcgccgccta ccgcgaggcc gcggccgggc
    12181 tcctcgacgg cgggagcgac ctcctcctgg tcgagacggt cttcgacacc ctcaactgca
    12241 aggcggcgct cttcgcggtc gaggaggaat tcgccgcccg cgggcatcgc gtgccggtga
    12301 tggtctcggt caccatcacc gaccggagcg gccgcacgct gtccgggcag gccccggagg
    12361 cgttctggac ctcgatcgcc cacgcgcgcc cgttctcggt ggggctcaac tgcgccctcg
    12421 ggcccaggga gcttgccccc cacctccgcg agctctcccg cgtgagcccg gcgttcgtct
    12481 cgatccaccc caacgccggc cttcccaacg ccttcggcgg ctacgacgag acgcctgccg
    12541 cgatggcctc ggtcatcggc gactgggcgg cccaggggct cgtcaacatc gtcggcggct
    12601 gctgcggcac gaccccggaa cacatcgccg cgatcgcgga tgcggtccgg ggccgcgcgc
    12661 cccggcgccc gcccgccatc gagccgcgcc tgcggctggc cggcctcgag cccttcaccc
    12721 ttggccccga cacgctcttc gtcaacatcg gtgagcgcgc caacgtcacc ggctcccgcc
    12781 gcttcgcccg gctcatcgag gcaggcgact acgccgccgg actcgaggtg gcgcgccagc
    12841 aggtggcaag cggcgcgcag atgctcgacc tcaatttcga cgaggcgatg ctcgactcgc
    12901 cccgggcgat ggagcgcttc ctccgcctcc tcgccgccga accggacatc tcgcgggtgc
    12961 cggtggtgct cgactcgtcg aagtggagcg tcatcgaggc ggggctcaag aacgtccagg
    13021 ggaagccggt ggtcaactcg atctcactca aggagggcga gtccgagttc ctccgccagg
    13081 cgcggctggc gcggcgctac ggcgcagcgg tcatcgtgat ggccttcgac gagcggggcc
    13141 aggccgacac cgtcgagcgc cgggtcgagg tcctcacgcg cgcctgtcgc ctcctggtgg
    13201 acgaagcggg catgccgccc gaggacgtga tcgtcgaccc gaacatcttt gcgatcggca
    13261 ccgggctgcc ggaacatgcc cgctacgccg tcgactacct ggaggccacc cgcaggatca
    13321 aggcggcgct ccccgcggtc cacgtgagcg gcggcctcag caacctctcc ttcgccttcc
    13381 gcggcaacga tgtcatccgc gaggcgatcc actcggtctt cctcttccac gccatccagg
    13441 ccgggatgga cctcggcatc gtcaacgccg gggcgctccc gatctacgag gacatccctc
    13501 ccgacctccg ggaacgggtg gaggatctcg tgctcgaccg gaggcccgac gccaccgagc
    13561 gcctcctggc cgtggcggac gccgtgaagg ggcgcgccgc cgccgccacc gtcgacgccg
    13621 agtggcgcac gctcccggtc cacgagcgcc tcgcccacgc gctggtccac ggcatcaacc
    13681 aattcatcgt ggccgacacc gaggaggccc ggcagggcgc gtcccacccg gtggaggtca
    13741 tcgagggacc gctgatggcg gggatgaacg tcgtcggcga cctcttcggc gccgggaaga
    13801 tgttcctgcc ccaggtggtc aagagcgcca gggtcatgaa gcaggcggtg gcccacctgg
    13861 tgcccttcct cgaggccgaa aaggcgcgaa atcccggcac ccgccccgcg ggaagggtcg
    13921 tcctcgccac cgtgaagggg gacgtccacg acatcggcaa gaacatcgtg ggcgtggtcc
    13981 tgcagtgcaa caactacgag gtgaccgatc tcggcgtgat gacgccgtcg gaccggatcc
    14041 tcgatgccgc gcgcgagtcg ggcgccgaca tcatcggctt gagcgggctc atcaccccgt
    14101 cgctcgggga aatgagcttc gtggcatccg agatggaacg cgagggattc cgcatcccgc
    14161 tcctcatcgg cggtgccacc acctcgcgca tccacactgc ggcgcgcatc gccccgaagt
    14221 actccggccc ggtggtccac gtggcggatg cctcccgcgc cgtggcggtc gcgagcaccc
    14281 tcctcaaccc cgcggagcgc ggagcgttcg ccgagcggac ggcagcggag taccgcggcc
    14341 tggcccggga gcacgcggaa cgcgggagcg ccgctcgccc cacgatcgcg gaggcgcgcg
    14401 cgcgccggcc gacggtggac acgtcccgcc cggcgccgcg cccctcattc ctcggcgttc
    14461 ggtcgctcgc gccctggccc ctccccgaac tggtcgagcg gatcgactgg acccccttct
    14521 tccagacctg ggaattgaag ggacgctacc ccgacatcct ccgcgatcct tccgtgggcg
    14581 agaccgcccg ctccctccac gccgacgcgc tggcgctgct cgatcgcatc gtgcgggagg
    14641 ggctgctgga agcccgcgcg gtgtatggct tcttcctggc cgaacgcgcg ggggacgacg
    14701 acatcgcgct cctgtcaccc gatg
//
LOCUS       DASUAP010000179         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_631984, whole genome shotgun sequence.
ACCESSION   DASUAP010000179 DASUAP010000000
VERSION     DASUAP010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631984"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1675
                     /locus_tag="VFU45_04490"
     CDS             113..1675
                     /locus_tag="VFU45_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEU5358354.1"
                     /translation="MTLPGPAPRERLAAIDVGSNSIRLVVAEHDPAAGLTIIDEVKDQ
                     PRLARGLAATGVLDDVAMARALETLDRMREVCERRGVRRISAVATAAVRDAANGALFL
                     DKVQKRLGLPLRVIDADEEARLSFRSVARHFRLEQGRSVVADIGGGSLELVSAMKGVI
                     QRKVSLPYGAVRLTERFTLDQPGARKRVDLLRAEVRKSLRKTLPPREWAGGTVIGSGG
                     TYTNLARMAVVRRTGAVPEAIHGTVVTASEVEQLLEWLVELGPERRRQVAGLSAHRAD
                     IIVAGLAVTIELLEQLEAREVAVSAYGLREGLLLEMVGAETPAPAERDPLQLAREFVE
                     RCQGDRRHVEQVRRLALQLFEQLGEKVGCAREERALLEYAALLHDVGQLVSYRGHHKH
                     SAQLILNADRLGFSAHDRVLVALISRYHRKSGPSKKHEAFAALSKEDQGIVRRLSGLL
                     RLADGLDRGHTGAVETVRTRLTRDKLTVTVAPRLARADLGLEVWAADRKSDVLAKVLG
                     REVEVRTVKSEK"
     gene            complement(1836..>3676)
                     /locus_tag="VFU45_04495"
     CDS             complement(1836..>3676)
                     /locus_tag="VFU45_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5358355.1"
                     /translation="ALLFAEIVEEAGLPPGVVNIITGDGRTGEALVRHPGVDKIAFTG
                     STEVGRLIRVATAGSGKKLSLELGGKSPFIVFENADLDSVVEGVVDAIWFNQGQVCCA
                     GSRILVQEGVAEKLVRKLRARMETLRVGDPLDKAVDIGAIVAPVQLRRIEKLVKQGEE
                     EGAVRWQPSWSCPTDGWFYPPTLFTNVSPAATIAQEEIFGPVVVLMTFRTPAEAVELG
                     NNTRYGLAASVWTDNLNLALDVAPKLKAGTVWINCTNMFDAASGFGGYRESGFGREGG
                     IEGLKEYVKPAWMTGGRTDGRSHGRKAAKWMDPTTKAAGSSRHVRASVRPSAPPPIDR
                     TAKLFIGGKQARPDSGYSTPVHAPDGTLVGEVGTGNRKDIRNAVEAARKAAEGWATAT
                     AHHRAQILYYVAENLSVRAQEFAGRIRRVTGATPGEAAREVEASIGRLFTYAAWADKW
                     DGRVHHTPYRNVTLAMPEAIGVVGIACPREAPLLGLVSLVAPAVALGNATVVVPSERA
                     PLLATDLYQVLETSDLPAGVINIVTGARDELAQVLAAHDDVDAMWYFGSAKGSADVER
                     LSAANLKRTWVTYGRGRDWYAAGQGEGEEFLDQATQVKNIWVPYGE"
BASE COUNT          535 a         1360 c         1239 g          542 t
ORIGIN      
        1 tggccgacct ggccaccaac atcggcgagg acgtggtgtt cctggtcgag gggaagtcga
       61 tcaagcacca cgctgaagac cggggcgacg cccccaaggc gccggcggca ggatgaccct
      121 ccccggcccg gcaccgcggg agcggctcgc cgccatcgac gtcggatcga actcgatccg
      181 gctggtggtg gcggagcacg acccggcggc cgggctcacg atcatcgacg aggtcaagga
      241 ccagccgcgg ctcgcccgcg ggctcgcggc cacgggcgtc ctcgacgacg tggcgatggc
      301 ccgcgcgctc gagaccctcg accggatgcg cgaggtctgc gagcggcgcg gcgtccggcg
      361 gatctcggcg gtcgcgaccg cggcggtgcg ggatgcggcc aatggcgcgc tcttcctcga
      421 caaggtgcag aagcgcctcg ggctcccgct ccgcgtcatc gacgccgacg aggaggcgcg
      481 gctctccttc cgctcggtgg cgcgccactt ccgcctggaa caaggccgca gcgtcgtcgc
      541 cgacatcggc ggcggttcgc tcgaactggt gagcgccatg aagggcgtca tccagcgcaa
      601 ggtctccctc ccctacggcg ccgtccggct gaccgagcgc ttcaccctcg accagccggg
      661 cgcccgcaag cgcgtcgacc tgctccgcgc cgaggtgcgg aagtcgctcc ggaagacgct
      721 cccgccccgc gagtgggccg gcgggacggt catcggctca ggtgggacct acaccaacct
      781 ggcacggatg gcggtggtcc ggcgcaccgg cgccgtcccc gaggcgatcc acggcaccgt
      841 cgtcaccgcc agcgaggtgg agcagctcct cgaatggctg gtggagctcg gccccgaacg
      901 ccggcgccag gtggccgggc tctccgccca ccgggcggac atcatcgtcg cgggcctcgc
      961 ggtgacgatc gagctcctgg agcagctcga agcccgtgag gtcgcggtca gcgcctacgg
     1021 gctccgcgaa gggctcctgc tcgagatggt gggcgctgag acgccggccc ccgccgagcg
     1081 cgacccgctc cagctcgcgc gggagttcgt cgagcgctgc cagggcgacc gccgccatgt
     1141 ggagcaggtg cgccgcctgg cgctccagct ctttgagcag ctcggcgaga aggtcggctg
     1201 cgcccgggag gagcgcgcgc tcctcgagta cgccgccctc ctccacgacg tgggccagct
     1261 ggtgagctac cgcggccacc acaagcacag cgcccagctc atcctcaacg ccgaccgcct
     1321 cggcttcagc gcccacgacc gcgtgctcgt ggcgctcatc agccgctacc accggaagtc
     1381 gggcccgtcg aagaagcacg aggccttcgc cgcactctcg aaggaggacc aggggatcgt
     1441 ccgccggctg tcagggctgc tgcgcctggc cgacgggctc gaccgcggcc acaccggcgc
     1501 cgtcgagacg gtccgcaccc ggctcacgcg cgacaagctc accgtcaccg tggccccgcg
     1561 gctcgcccgc gccgacctgg ggctcgaggt ctgggccgcc gaccgcaaga gcgatgtgct
     1621 ggcgaaggtg ctggggcggg aagtcgaagt acggacagtg aagagtgaaa agtgaagagg
     1681 gaagagggga aagtgagaag ggaaaaggga agagggaaaa gtgagaagtg aaaagtgaag
     1741 aggggactcc gctgttgagc gagccccctt tttccctttt cactcttccc ttttcccttt
     1801 tcactcttcc cttctccctt ttcactcttc acttctcact ccccgtacgg cacccagatg
     1861 ttcttgacct gcgtcgcctg gtcgaggaac tcctctccct ccccctgccc agcggcatac
     1921 caatcgcgcc cgcggccgta ggtcacccag gtgcgcttga gattcgccgc ggagagccgc
     1981 tcgacgtcgg cgctcccctt ggccgaaccg aagtaccaca tcgcgtcgac gtcgtcgtgc
     2041 gccgcaagga cctgcgccag ctcgtcgcgc gcgccggtca cgatgttgat gacgccggcc
     2101 gggaggtccg aggtctccag cacctggtag aggtcggtgg cgagcagcgg cgcacgttct
     2161 gacggcacca ccacggtggc attgccgagc gccacggccg gcgccaccag cgacaccagc
     2221 cccaggagcg gcgcctcccg cgggcaggcg atccccacca cgccgatggc ctccggcatc
     2281 gcgagcgtga cattgcggta cggggtgtga tgcacccggc cgtcccactt gtcggcccac
     2341 gccgcatagg tgaagaggcg gccgatcgac gcctcgacct cgcgcgctgc ctcgccgggc
     2401 gtggcgccgg tgacgcgccg gatccgtccc gcgaactcct gtgcccggac gctcaggttc
     2461 tcggccacat aatagaggat ctgcgcccgg tggtgcgccg tggcggtggc ccagccctcc
     2521 gctgccttcc gcgccgcctc gacggcgttc cggatgtcct tccggttccc ggtgccgacc
     2581 tcgccgacga gcgtgccgtc gggcgcatgc acgggcgtgc tgtaccctga gtcagggcgc
     2641 gcctgcttgc cgccgatgaa gagcttcgcc gtgcggtcga tgggaggagg ggcggacgga
     2701 cgcacagacg cacggacgtg ccggctcgat cctgccgcct tcgtcgtcgg gtccatccac
     2761 ttggcggcct tgcgcccatg cgaccgcccg tccgtccgtc cccctgtcat ccacgccggc
     2821 ttcacgtact ccttgagccc ctcgatcccg ccctcgcgcc cgaagccgct ctcccggtag
     2881 ccgccgaagc cgctcgccgc gtcgaacatg ttggtgcagt tgatccagac cgttccggcc
     2941 ttgagcttgg gcgccacgtc gagggccagg ttcaggttgt cggtccagac gctcgccgcc
     3001 aggccgtacc gggtgttgtt gccgagctcc accgcctcgg cgggggtgcg gaaggtcatc
     3061 agcaccacca cgggcccgaa gatctcctcc tgcgcgatgg tggcggcggg ggacacgttc
     3121 gtgaagagcg tcggcgggta gaaccagccg tcggtcgggc agctccacga cggctgccag
     3181 cggaccgcgc cctcctcctc tccctgcttg accagcttct cgatccgccg caactggacc
     3241 ggcgccacga tcgcgccgat gtccactgcc ttgtcgagcg ggtcgccgac gcgaagggtc
     3301 tccatccgcg cacgcagctt ccgcaccagc ttctcggcca cgccctcctg gaccaggatc
     3361 cgcgagccgg cgcagcagac ctgcccctgg ttgaaccaga tcgcgtcgac cacgccctcg
     3421 accacgctgt cgaggtcggc gttctcgaac acgatgaagg gcgacttccc gccgagctcg
     3481 agcgacagct tcttgccgct ccccgccgtc gcgacgcgga tgagccgccc cacctcggtc
     3541 gagccggtga aggcgatctt gtcgaccccc gggtgccgga ccagcgcctc gccggtgcgc
     3601 ccgtccccag tgatgatgtt gaccacgccg ggcgggagtc ccgcctcctc cacgatctcc
     3661 gcgaagagca gcgccg
//
LOCUS       DASUAP010000180         3998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_413668, whole genome shotgun sequence.
ACCESSION   DASUAP010000180 DASUAP010000000
VERSION     DASUAP010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413668"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1176)
                     /locus_tag="VFU45_04500"
     CDS             complement(85..1176)
                     /locus_tag="VFU45_04500"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU5358356.1"
                     /translation="MSESFAGLLGRFDVPGEFVAMEPLPGGYINDSYRVTLAADGART
                     EWLLQRINPTVFTDPDALMGNIAHVTRHMSARLRDDGVADWRRRTLVVIPTREGASHH
                     RGVDGAAWRLFPFIESATVRTEAASPELAREAARAFGEFLRLVADYDGPPLRETIRGF
                     HDTAARFRALVQAVRADPVARAAQAGPEIRLALEYQAMADVLPPLMRRGVVPVRVAHN
                     DAKIANVLFEVDSGRALAVVDLDTVMPGSALHDFGDLVRSSVSASAEDETDLAKVDVR
                     VPWFRALVEGYLSEAGGVLTPDERRLLVFAGRLITLEQAVRFLTDHLQGDRYYRTTRA
                     GQNLDRARTQFRLFERLTERSDELEATVG"
     gene            complement(1173..2075)
                     /locus_tag="VFU45_04505"
     CDS             complement(1173..2075)
                     /locus_tag="VFU45_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU5358357.1"
                     /translation="MPNRRQTPTLVVLAAGMGSRYGGLKQLDPVGPNGATLMDYSVFD
                     ARRAGFGKVVFVIRPDMEEAFRPFVSERYGSSIDVATAHQRREDIPAGVSAPAERTKP
                     WGTGQAVLAAETEVSGPFAVINADDFYGRAAYEGLAGFFGSSRDDTFALVGYRLSDTL
                     SESGGVNRGVCRVTADGWLDGIEEMYEIQRRADGTYAGRGSLPLNDRTLVSMNFWGFT
                     PAVFGLLRRGFTDFFHSADLLKGEYLLPTEIDRAIHAGRVKVKVLDAGSRWFGMTYPA
                     DRPAVSAAIHRLVAEGAYPERLGG"
     gene            2198..2551
                     /locus_tag="VFU45_04510"
     CDS             2198..2551
                     /locus_tag="VFU45_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358358.1"
                     /translation="MYEHRSKPMLTQGQFIRRMLGHLAFSAGLVAISLFAGMWGYHHY
                     EGLSWLDGFVNASMLLGGMGPVNEIHTDAGKLFAGCYALYAGLVFLIVAGVLLAPVIH
                     RIAHKIHLVEEEGDD"
     gene            2548..3060
                     /gene="lspA"
                     /locus_tag="VFU45_04515"
     CDS             2548..3060
                     /gene="lspA"
                     /locus_tag="VFU45_04515"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU5358359.1"
                     /translation="MTSPSIKSRIFAAVAVTFLVADRITKHLAEASLTPHLPRRVAGD
                     VVRLTLTWNQSSAMGLFSFGAWSRWIFAAIAVAMLGVLVALLREAPATARWRAAALGA
                     VAGGAVGNLVDRILSGRGVTDFIDVGTSGWRFWTFNVADAGVTCGALLLALLLWAEER
                     EQKRRAEAGQ"
     gene            3084..3932
                     /locus_tag="VFU45_04520"
     CDS             3084..3932
                     /locus_tag="VFU45_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358360.1"
                     /translation="MASAVIAVSASAQVPFDGCVDRGDHPVQAIVRNDLGGYAGMATV
                     VQGKPVIYWNAHAAGASDNAFRVFLYLHECAHIRLRHVYDGSESLEIEKEANCWAMQL
                     MLDGGMLDGYSEGVLWDDIKNIRGDATHLTGDAAVASLRACLAARLDRGHWDVMLDSL
                     ALAATDSFKAIRGPLIREITPGPVYESRLSAPGVWDCDITEPAELRCPLFAARTERLS
                     KQRFKAMDKIIRKWLDSDWTATDRSAPQPGIAQELLAQNGRTGVLLTLVRTSAGRIWL
                     IAEPTS"
BASE COUNT          625 a         1313 c         1437 g          623 t
ORIGIN      
        1 ctgctgcgac ttcacgtgac cccctgactc tattcccttg gctctccccc tctcccgtaa
       61 gggagagggg gtcggggggt gaggtcagcc cacggtcgct tccagctcgt cggatcgctc
      121 ggtcagccgt tcgaaaagcc ggaactgggt gcgcgcgcgg tcgaggttct ggccggcgcg
      181 ggtggtgcga tagtaccggt cgccctgcag gtggtcggtc aggaagcgca ccgcctgctc
      241 gagggtgatg agccggccgg cgaagacgag gagccgccgc tcgtcagggg tgagcacacc
      301 gccggcctcg ctcaggtacc cctccaccag cgcccggaac caggggaccc gcacatccac
      361 cttggcgagg tccgtctcgt cctccgccga ggcgctcacc gacgagcgca cgaggtcgcc
      421 gaagtcgtgg agcgccgagc ccggcatcac ggtgtcgagg tccacgaccg cgagggcccg
      481 cccgctgtcg acctcgaaga gtacgttggc gatcttggcg tcgttgtggg ccacccggac
      541 cggcaccacc cctcgtcgca tgagcggagg caggacgtcg gccatcgcct ggtactcgag
      601 cgccaggcgg atctccggtc ctgcctgggc cgcgcgcgcg acggggtcgg cgcggacggc
      661 ctgcaccagg gcccggaagc gggcggcggt gtcgtggaag ccgcggatgg tctcgcggag
      721 cggagggccg tcgtaatcgg ccacgaggcg caggaactcg ccgaaggcgc gcgccgcttc
      781 ccgcgccaac tcgggcgacg cggcctcggt gcggacggtg gcgctctcga tgaacgggaa
      841 gagccgccac gccgcgccgt cgacgccccg atggtggctc gcgccctcgc gggtggggat
      901 gaccaccagg gtgcggcgcc gccagtcggc cacgccgtca tcccgcaggc gggcggacat
      961 gtgccgcgtc acgtgcgcga tgttgcccat cagcgcgtcg ggatcggtga agacggtggg
     1021 gttgatccgc tgcagcagcc actccgtccg ggccccatcc gccgcgagcg tcacccggta
     1081 cgaatcgttg atgtacccgc cggggagggg ttccatggcc acgaactcgc ccggaacgtc
     1141 gaagcgccca aggagcccgg cgaacgactc gctcatccgc cgaggcgctc ggggtaggcg
     1201 ccctcggcca cgagccggtg gatcgcggcg ctgaccgcgg ggcggtcggc cggatacgtc
     1261 atcccgaacc agcggcttcc ggcatcgagc accttcacct tgacgcgccc ggcgtggatg
     1321 gcgcggtcga tctcggtggg gaggagatac tcgcccttga ggaggtcggc cgaatgaaag
     1381 aagtcggtga acccgcggcg gaggagcccg aagacggccg gagtgaatcc ccagaagttc
     1441 atcgatacga gggtccggtc gttgagcgga aggctcccgc ggccggcgta ggtgccgtcg
     1501 gcgcggcgct ggatctcgta catctcctcg atgccgtcga gccatccgtc ggcggtcacc
     1561 ctgcagacgc cgcgattgac gccgcccgat tcggagagcg tgtcggaaag gcggtagccg
     1621 acgagcgcga atgtgtcatc ccgggacgag ccgaagaatc cggccagtcc ttcgtacgcg
     1681 gcccggccgt agaagtcgtc ggcgttgatg accgcgaagg gccccgatac ctccgtttcc
     1741 gcggcaagca ccgcctggcc cgtcccccac ggcttggtcc gctcggccgg cgcgctgacc
     1801 ccggcgggga tgtcctcccg ccgctggtgg gcggtggcca cgtcgatcga ggaaccgtac
     1861 cgttcggaga cgaagggccg gaacgcctcc tccatatcgg gacggatgac gaagaccacc
     1921 ttgccgaagc cggcgcggcg ggcgtcgaag accgaatagt ccatcagggt ggcgccgttg
     1981 gggcccacgg ggtcgagctg cttgaggcca ccgtaccggc tgcccattcc ggcggcaagg
     2041 acaacgagcg taggagtttg acgtcgattc ggcatcggga gcgctatata aggaagggga
     2101 aggcccaata taccgatcgc ggcccggatc gggcgagagg cagcacgggc gtccagcgct
     2161 taagttagtc catccacgac gatccccggg aggacgggtg tacgaacacc gttcgaagcc
     2221 gatgctcacg caggggcaat tcatccggcg gatgctgggc cacctggcgt tctccgccgg
     2281 cctggtggcg atctcgctgt tcgccgggat gtgggggtac caccactacg aggggctgag
     2341 ctggctcgac ggcttcgtca acgcgtcgat gctgctgggc gggatggggc cggtcaacga
     2401 gatccacacc gatgccggga agctttttgc cgggtgctac gcgctctacg ccgggctcgt
     2461 cttcctcatc gtggcgggcg tgctcctcgc cccggtgatc caccggatcg cgcacaagat
     2521 ccacctggtc gaggaagagg gagacgattg acctcgccga gcatcaagtc gcggatcttc
     2581 gcggccgtcg ccgtcacctt cctcgtggcc gaccggatca ccaagcacct cgccgaggcg
     2641 agcctgacgc cgcatttgcc gcggcgggtg gcgggcgatg tcgtgcggct gacgctcacc
     2701 tggaaccaga gctcggcgat ggggctcttc tccttcggcg cctggtcgcg ctggatcttc
     2761 gccgccatcg cggtcgcgat gctcggcgtg ctggtggcgc tcctgcgcga ggcgcctgcc
     2821 accgcacgct ggcgcgccgc cgcgctcggc gcggtcgcgg gcggggccgt gggcaacctg
     2881 gtcgaccgca tcctctccgg ccgcggcgtc accgatttca tcgacgtggg gacgtcgggg
     2941 tggcggttct ggacgttcaa cgtggccgac gccggcgtga cctgcggcgc actgctgctg
     3001 gcgttgctcc tgtgggcgga ggagcgggag cagaagcggc gggcggaggc gggtcagtga
     3061 tgcgcgcggc atggacggtg gcgatggcct ccgccgtcat cgccgtatcg gcatcagcgc
     3121 aagtcccgtt cgatggctgc gttgaccgag gggaccatcc ggtccaggcg atcgttcgca
     3181 atgacctggg tggttatgcc ggaatggcga ccgtggtaca gggcaaaccc gtcatatact
     3241 ggaacgcgca cgccgcagga gcgagtgaca acgcgttccg ggtgttcctg tacctgcacg
     3301 agtgtgcgca cattcgactg cggcatgtct acgacggcag cgaatcgctc gagatcgaga
     3361 aggaagcgaa ttgctgggcg atgcagctga tgctcgacgg tgggatgctg gacgggtatt
     3421 ccgaaggcgt cctgtgggat gacatcaaga acatacgagg tgacgccaca cacctgaccg
     3481 gcgatgctgc ggtggcgagc cttcgggcct gcctggcggc gcggctggat cgaggacatt
     3541 gggacgtcat gctcgattcg ctggcgcttg ctgcgacgga ctcattcaag gcgatccgtg
     3601 gcccgctcat ccgggagatc accccaggcc cggtatacga atccaggctc agcgcgcccg
     3661 gcgtgtggga ttgtgacatc accgaacccg ccgagctccg gtgccccctc tttgcggcgc
     3721 gcaccgaacg cctctccaag cagcggttca aggcgatgga caagatcatc cggaaatggc
     3781 tggattccga ctggaccgcc accgaccggt cggcacccca acccggcatc gcccaggaac
     3841 tgctggcaca gaatggacgg accggggtgc tgctgacgct cgtgcggacg tcggccgggc
     3901 ggatctggct catcgcggag cccacgagct gagcggtgga tctggccgga caacatggac
     3961 gtccaccgac cgtttcctcc ctcgctcggg aggggcat
//
LOCUS       DASUAP010000181         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1272293, whole genome shotgun sequence.
ACCESSION   DASUAP010000181 DASUAP010000000
VERSION     DASUAP010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272293"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /gene="phoU"
                     /locus_tag="VFU45_04525"
     CDS             complement(<1..472)
                     /gene="phoU"
                     /locus_tag="VFU45_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU5358361.1"
                     /translation="MSQDTHRHFHDELSHVKVRLLTMSGEAEAALALAVEALLERNAA
                     KAKRVITGDRAIDALEVEIEEKCIELLGLQQPMARDLRMLTAALKIANDLERVGDHAV
                     NIAQSAERLTESRPIAPEPELVEMARLAREMLADALEAFVRSDAAAARDVCRRDD"
     gene            complement(469..1236)
                     /gene="pstB"
                     /locus_tag="VFU45_04530"
     CDS             complement(469..1236)
                     /gene="pstB"
                     /locus_tag="VFU45_04530"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEU5358362.1"
                     /translation="MADPIPHIATERLTALYGKVAAVKDVTIAFKPNTVSALIGPSGC
                     GKSTFLRSLNRMHELTEGGWITGKVLLDGEDVYTSRINPTVLRRRVGMVFQKASPFPT
                     MTIAQNVSAGPALTGQVRKRDLPELVERCLRQAALWDEVKDRLDQPATGLSGGQQQRL
                     CIARSLANEPEVLLLDEPCSALDPISTQRIEELLLEIKKDYTIILVTHNLQQAARASD
                     YTAFFDAGRLVEYGETRRLFTTPAEERTEAYITGRFG"
     gene            complement(1229..2074)
                     /gene="pstA"
                     /locus_tag="VFU45_04535"
     CDS             complement(1229..2074)
                     /gene="pstA"
                     /locus_tag="VFU45_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU5358363.1"
                     /translation="MIRERIRWAKNHAMIGLLAAAVVLALLPLGFILAHLVAKGASSL
                     DWNFFTKLPAPAGESGGGVYHAIVGTLIIVGIAAAIGLPFGIGAGVYCTEYPSHRLAS
                     VTRFVADVMNGTPSIVVGVFAWTWIVARQKHFSGLAGSVALALIMIPMVMRTTEEMIK
                     LVPHALREAALGLGIPRWRTGVTIVLRTALPGIVTGSLLAVARVSGETAPLLFTALGS
                     QYLTLDPRQPMAALPLVVFNFATGPYEDWHRMAWAASLVLLLVVLVLSITARLATRRR
                     STHRG"
     gene            complement(2071..>2606)
                     /locus_tag="VFU45_04540"
     CDS             complement(2071..>2606)
                     /locus_tag="VFU45_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5358364.1"
                     /translation="TVFPFLRQTLGFLPFFQGPIYGPSMLAAAVILAIMILPFIMSVA
                     REVLTAVPGAQREAALALGATRWEAVATAVVPYARSGIVGAGILGLGRALGETMAVTM
                     LIGNTHDISASLFAPGYTMAAVIANEFSEAATPMHFAALTYVALALFGLTVLVNAGAR
                     LLIWRVARGSGAGSRAL"
BASE COUNT          392 a          866 c          988 g          360 t
ORIGIN      
        1 ggtcgtcccg ccggcagacg tcgcgcgcgg cggcggcgtc gctccggacg aacgcctcga
       61 gcgcgtcggc cagcatctcg cgggccaggc gcgccatctc gacgagctcg ggttcgggcg
      121 cgatcggccg ggactcggtg agccgctcgg ccgactgcgc gatgttgacc gcgtggtcgc
      181 ccacccgctc gaggtcgttg gcgatcttga gcgcggcggt gagcatccgg aggtcgcggg
      241 ccatcggctg ctgcaggccc aggagctcga tgcacttctc ctcgatctcg acctcgaggg
      301 cgtcgatcgc ccggtcgccg gtgatgaccc gcttggcctt ggccgcgttg cgctcgagca
      361 gcgcctccac cgcgagggca agggccgcct cggcctcgcc cgacatggtg aggagccgca
      421 ccttgacgtg gctcagctcg tcgtggaagt ggcggtgcgt gtcctggctc atccgaacct
      481 ccccgtgatg tacgcctcgg tgcgctcctc cgccggggtg gtgaacaacc gccgggtctc
      541 gccgtactcg acgagccggc ctgcgtcgaa gaacgcggtg taatccgacg cgcgggccgc
      601 ctgctgaagg ttatgcgtga cgaggatgat ggtgtagtcc ttcttgatct cgaggagcag
      661 ctcctcgatc cgctgggtgg agatcgggtc gagcgcggag cacggttcgt cgaggagcag
      721 cacctcgggc tcattggcca gcgaccgcgc gatgcagagc cgctgctgct ggcccccgga
      781 gagcccggtg gccggctggt ccaggcggtc cttgacctcg tcccagagcg cggcctggcg
      841 gaggcagcgc tccaccagtt ccgggaggtc gcgcttgcgc acctggccgg tgagcgccgg
      901 ccccgccgag acgttctggg cgatggtcat cgtggggaag ggcgaggcct tctggaacac
      961 catccccacc cgccggcgga gcacggtggg gttgatccgg gaggtgtaga cgtcctcgcc
     1021 gtcgaggagc accttgccgg tgatccagcc cccctcggtc agttcgtgca tccggttgag
     1081 cgagcgcagg aaggtggact tgccgcagcc ggacggcccg atcagggcgc tcacggtatt
     1141 gggcttgaac gcgatcgtca cgtccttcac cgcggcgacc ttgccgtaga gcgcggtaag
     1201 gcgttcggtg gcgatgtgcg ggatcgggtc agccacgatg cgtggacctc cggcgcgtcg
     1261 caagtcgcgc ggtgatgctg aggacgagga cgacgagcag gagcaccagc gacgccgccc
     1321 aggccatgcg gtgccagtcc tcgtaggggc cggtcgcgaa gttgaagacg acgagcggga
     1381 gcgccgccat cggctggcgg gggtcgagcg tcaggtactg gctgccgagc gcggtgaaga
     1441 ggaggggggc cgtctcgccc gagacgcgcg ccaccgcgag gaggctcccg gtcacgatac
     1501 ccgggagtgc cgtccggagc acgatggtga ccccggtccg ccagcggggg atgccgaggc
     1561 cgagggccgc ctcgcggagc gcgtggggga cgagcttgat catctcctcg gtggtgcgca
     1621 tgaccatcgg gatcatgatc agcgccagcg ccacgctgcc ggccaggccc gagaagtgct
     1681 tctgccgcgc cacgatccag gtccaggcga agacgccgac cacgatcgac ggggtgccgt
     1741 tcatcacgtc ggccacgaag cgggtgacgc tcgccagccg gtgggacgga tactcggtgc
     1801 agtagacgcc ggcgccgatg ccgaacggga ggccgatcgc cgcggcgatg cccacgatga
     1861 tgagcgtccc gacgatggcg tggtacacgc cgccgcccga ctcgccggcc ggggcgggga
     1921 gcttggtgaa gaagttccag tcgaggctcg aggcaccctt ggccacgagg tgggccagga
     1981 tgaacccgag cggcaggagg gccagcacca cggccgccgc caggagcccg atcatggcgt
     2041 ggttcttggc ccagcggatg cgctcgcgga tcacagggcc cggctcccgg cgccggagcc
     2101 gcgcgccacc cgccagatga ggagccgggc gccggcgttg accagcacgg tgaggccgaa
     2161 gagcgcgagc gcgacgtagg tgagcgccgc gaagtgcatc ggcgtcgccg cctccgagaa
     2221 ctcgttggcg atcaccgccg ccatcgtgta gcccggcgcg aagagcgagg cggagatgtc
     2281 gtgggtgttg ccgatgagca tggtgacggc catcgtctcg ccgagggcgc ggccgagccc
     2341 gaggatcccg gcgccgacga tcccggaacg ggcgtacggg accaccgccg tggccaccgc
     2401 ctcccagcgg gtggccccga gggccagcgc ggcctcgcgc tgggcgccgg ggaccgccgt
     2461 cagcacctcg cgcgccaccg acatgatgaa cggcaggatc atgatcgcga ggatcaccgc
     2521 cgcggcgagc atcgacgggc cgtagatcgg cccctggaag aacgggagga acccgagcgt
     2581 ctggcgcagg aaggggaaca ccgtgc
//
LOCUS       DASUAP010000182         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_182475, whole genome shotgun sequence.
ACCESSION   DASUAP010000182 DASUAP010000000
VERSION     DASUAP010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182475"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFU45_04545"
     CDS             <1..1215
                     /locus_tag="VFU45_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358365.1"
                     /translation="TADVRHITQVAETLGQAHDHVLVLGIGGSALGARALLSALRRPA
                     WNEHSDEGREFYPRLTILDNVDPTSVADALHRIDPRRVLVNVISKSGGTAETLAQYLI
                     VREWLEGALGAAATRHLVFTTDPAKGALRDIGRREGIAMLDVPPGVGGRFSVLSPVGL
                     LPAALVGIDVGALLAGARTAVAAAAGDDLLHNPAALFAALLHAADTGHGARIHALMPY
                     SDRLRDVAEWYRQLWAESLGKRVGRDGSEVFAGPTPLAAVGATDQHSQVQLFMEGPFD
                     KVVTFVAVDDPGGDVTIPAREGLPPDLAYLAGHTLGGLLAAEREATAAALAQHGRMNC
                     TLRLPDLSPESIGELLMFLQLATGFAGAWYGVDPFDQPGVELAKVLTYGALGRPGFEG
                     RRTPAPPEGDVA"
     gene            1291..2706
                     /locus_tag="VFU45_04550"
     CDS             1291..2706
                     /locus_tag="VFU45_04550"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEU5358366.1"
                     /translation="MKDAMQQVVGYYGGAVLVTDGKVSVRDAAKLRSEATDRVVWDAV
                     FGDAALKEAARWLLWELGQAVGCRPASINGLYLARGRGECAGFTVPAINIRVLAYDSA
                     RAVFRAALAGKAGALLLEIARSEVAYTEQRPAEYVAVMIGAALREGYDLPLFIQGDHC
                     QVNAKKYQADAEAEVAEVRKLIGEEIGAGFYNIDVDTSTLVDLSHATLDAQQRLNYER
                     AAEFTRFIREEQPAGVTVSVGAEIGEVGHKNSTVEELRAFMQGYARSLAALGVQEGIS
                     KISVQTGTSHGGVVLPDGSIAKVKLDLDALAALSKVAREEYRMAGAVQHGASTLPSDA
                     FGNFPRVEACEIHLATNFQNIVFDHPRLPAPLKATMYRWLDQNAQGEKKPGDSDEQFY
                     YKARKKAIGPFKRELWSLPPEVRAAIAADLEKTFRFLFVQLKIDGTADLVRRHTTAPV
                     QHHAALKPAMALAEDDPDAGE"
     gene            2711..3079
                     /locus_tag="VFU45_04555"
     CDS             2711..3079
                     /locus_tag="VFU45_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5358367.1"
                     /translation="MTDDTAERGSSLPWFLLGLGLGAGLGILFAPRSGEETRASVRKR
                     LQSLRDVAGEQFEEAREAVVDRLDRVADVVAGEDEDDEEAPSRSTAREALQRRLHAAR
                     SRRRPAAAPGGDPEDEGPTT"
     gene            3217..>3968
                     /locus_tag="VFU45_04560"
     CDS             3217..>3968
                     /locus_tag="VFU45_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5358368.1"
                     /translation="MPFLLLVLIGLSTLVQGPRGVAAHDLLGAIDRFLPPSVGIAGRD
                     PSEVVRTLLGRVAQNRSRLSLYAIPLFIWFSTRLFASARTALNRIYRVATPPRKPRHF
                     VVSFLLAKAWDVLMVGATLLLFLVYILVSGAISLALTWGEAQATVTVAPFVAYLGRLA
                     GVFLTFGGALAFFLLVYRFGAARLLRWRTALGSATFAAVAFEVARRAFGLYLLSLAAG
                     NSPSVYANLGAAFLFILWTYFAALVFLLGGVVA"
BASE COUNT          530 a         1479 c         1376 g          583 t
ORIGIN      
        1 accgccgacg tccgccacat cacccaagtc gccgagaccc tgggccaggc gcacgaccac
       61 gtccttgtgc tcggcatcgg cgggtccgcc ctcggcgccc gtgcgctcct ctccgccctc
      121 cggcgtccgg cctggaacga gcacagcgat gaggggcggg agttctaccc ccggctcacc
      181 atcctcgaca acgtcgaccc gacctcggtg gccgatgcgc tgcaccggat cgacccgcgc
      241 cgggtgctgg tgaacgtgat cagcaagtcg ggcggcaccg ccgagacgct ggcccagtat
      301 ctcatcgtcc gcgagtggct cgaaggggcg ctgggagccg ccgcgacgcg ccacctggtg
      361 ttcaccaccg acccggccaa gggggcgctg cgcgacatcg gccgccgcga ggggatcgcg
      421 atgctcgacg tgccgccggg cgtggggggc cgtttcagcg tgctctcgcc ggtcggcctc
      481 ctgccggcgg cgctcgtggg gatcgacgtc ggggccctgc tcgccggcgc ccgcacggcc
      541 gtggccgcgg ccgcgggcga cgacctgctc cacaatccgg ccgcgctgtt cgcggcgctc
      601 ctccacgcgg ccgacacggg ccacggcgcg cgcatccatg cgctgatgcc ctattccgac
      661 cggttgcggg acgtggccga gtggtatcgc cagctctggg cggagagcct cggcaagcgc
      721 gtcgggcgcg acggatccga ggtgttcgcg ggaccgacgc cgctcgccgc cgtgggggcc
      781 accgaccagc acagccaggt ccagctcttc atggaggggc cgttcgacaa ggtggtcacc
      841 ttcgtcgcgg tggacgatcc cggcggcgac gtgacgatcc ccgcccgtga gggccttccg
      901 cccgacctcg cctacctcgc ggggcacacc ctcggcggcc tcctggccgc tgagcgggag
      961 gccacggctg cggcgctggc gcaacacgga cggatgaact gcaccctccg gctccccgac
     1021 ctgtcgccgg agagcatcgg tgaactgctg atgttcctcc agcttgccac cggcttcgcc
     1081 ggtgcctggt acggggtcga tcctttcgac cagccgggcg tggagcttgc aaaggtgctg
     1141 acctacggcg cgctcggccg gcccggtttc gagggccgcc ggaccccggc gccccccgaa
     1201 ggcgacgtgg cctgagccgg tggcggaggg gcgcgccgcc ccctaggttt cggcccggtc
     1261 cgggccatgt cgaacgcgcg aggtgcagcc atgaaggacg cgatgcaaca ggtggtgggc
     1321 tactacggtg gcgcggtgct ggtgaccgac ggcaaggtct ccgtccgcga cgccgccaag
     1381 ctccgctccg aggccaccga ccgggtagtg tgggacgccg tcttcggtga cgccgcgctc
     1441 aaggaagcgg cccgctggct tctgtgggag ctgggccagg cggtggggtg ccggccggcg
     1501 tccatcaacg gcctctatct ggcccggggg cggggcgagt gcgccgggtt caccgtcccc
     1561 gccatcaaca tccgggtgct cgcctacgac tcggcgcgcg ccgtcttccg agccgcgctc
     1621 gccggcaagg ccggcgccct gctcctcgag atcgcgcgca gcgaggtcgc ctacaccgag
     1681 cagcgccccg ccgagtatgt ggcggtgatg atcggcgccg cgctccgcga agggtacgac
     1741 ctcccgctct tcatccaggg cgaccactgc caggtgaacg ccaagaagta ccaggccgac
     1801 gccgaggcag aggtggccga ggtcaggaag ctgatcggcg aggagatcgg cgccgggttc
     1861 tacaacatcg acgtggacac gtcgaccctg gtcgacctct cccacgccac gctcgacgcg
     1921 cagcagcgcc tcaactatga acgtgcggcc gagttcactc ggttcatccg cgaggagcag
     1981 cccgccggcg tcacggtgtc ggtgggtgcc gagatcggcg aggtgggaca caagaactcc
     2041 accgtcgagg agctccgggc attcatgcag gggtatgccc gctccctcgc ggcgctcggc
     2101 gtgcaggagg ggatcagcaa gatctcggtg cagaccggga cgagccacgg cggcgtggtg
     2161 ctccccgacg ggagcatcgc gaaggtgaag ctcgacctcg acgcgctcgc ggcgctctcc
     2221 aaggtggcgc gcgaggagta ccggatggcg ggcgcggtgc agcacggggc cagcacgctg
     2281 ccgtccgacg cattcggcaa cttcccccgc gtcgaggcgt gcgagatcca cctcgccacg
     2341 aacttccaga acatcgtctt cgaccatccg cggcttccgg cgccgctcaa ggccacgatg
     2401 taccggtggc tggaccagaa cgcgcagggc gagaagaagc ccggcgactc ggacgagcag
     2461 ttctactaca aggcgcgaaa gaaggcgatc ggtcccttca agcgcgagct ctggtcgctg
     2521 ccaccggagg tccgcgccgc catcgcggcc gacctcgaga agaccttccg gttcctgttc
     2581 gtccagctca agatcgacgg caccgccgac ctggtgcgcc gccacacgac ggccccggtc
     2641 cagcaccacg ccgccctcaa gcccgcgatg gcgctggccg aggacgaccc cgacgcgggg
     2701 gaatgatccg atgaccgacg acaccgccga acgcgggagc tcgctcccct ggttcctgct
     2761 cgggctcggc ctgggcgcgg ggctcgggat cctctttgcg ccccggagcg gcgaggagac
     2821 ccgcgcgtcc gtacgcaagc gcctgcagtc cctgcgcgac gtcgccgggg agcagttcga
     2881 ggaggcccgc gaggcggtgg tcgaccggct cgatcgagtg gccgacgtcg tggccggcga
     2941 ggatgaggac gacgaggagg cgccgtcacg gagcaccgcc cgggaggccc tccagcggcg
     3001 cttgcacgcg gcccgcagcc gccgccggcc cgctgccgcc ccgggcggcg acccggagga
     3061 cgaggggccg accacctgac ctcggccgac ggtcgtccgg ccggcgagtg gcacgagacc
     3121 ccgtgggggt tcctccgccg cacgctctac gcggccggtg aggtcaacat cccgttcctc
     3181 gcgagcgcgc tcaccttcga cacgctcctc gcgggcctgc cgttcctcct gctcgtcctg
     3241 atcggcctgt ccaccctggt gcagggcccc cgtggcgtgg cggcgcacga cctcctcggc
     3301 gccatcgacc gcttcctccc gcccagcgtc ggcatcgccg gccgcgatcc gagcgaggtg
     3361 gtccgcacgc tgctcggtcg cgtggcccag aaccgcagcc ggctctcgct ctacgccatc
     3421 ccgctcttca tctggttcag cacccggctc tttgccagcg cgcgcaccgc actcaaccgc
     3481 atctaccggg tggccacgcc gccccgcaaa ccgcggcact tcgtcgtgag cttcctcctg
     3541 gccaaggcct gggacgtgct gatggtgggg gcgacgctgc tcctgttcct ggtctacatc
     3601 ctggtgtcag gcgccatcag cctggccctc acctggggcg aggcgcaggc caccgtcacg
     3661 gtggcgccgt tcgtcgcgta cctcggccgc ctggcgggcg tgttcctcac cttcggcggc
     3721 gcactcgcgt tcttcctgct ggtctaccga ttcggcgccg cccgcctcct ccgctggcgc
     3781 acagccctcg gttcggccac cttcgcggcc gtcgcgttcg aggtggcccg gcgcgccttc
     3841 gggctctacc tgctctcgct cgccgcgggc aactccccct cggtctacgc caacctcggc
     3901 gcggccttcc tcttcatcct ctggacgtat ttcgcggcgc tggtatttct cctgggcggc
     3961 gtcgtggc
//
LOCUS       DASUAP010000183         5136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_46231, whole genome shotgun sequence.
ACCESSION   DASUAP010000183 DASUAP010000000
VERSION     DASUAP010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46231"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /gene="chrA"
                     /locus_tag="VFU45_04565"
     CDS             <1..1141
                     /gene="chrA"
                     /locus_tag="VFU45_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEU5358369.1"
                     /translation="GSDAPGDRSSSGGGAAEVLGVFLRLGATAFGGPAAHVALMEEEF
                     VRRRRWLSRTELLDLVASAQLIPGPNSTELAIHLGYRRAGWRGLIAAGIGFILPAVLL
                     TALLAWAYARFGALVGVAGALAGAKAVAFVVVLRALWDFGRSALTTRWRIALSVGALA
                     ASLIGADELVILGVAVLLGLATVRRGAGAAAPAGYPLLARLAPALAGAPTLPALLLTF
                     LKFGAVLFGSGYVLLAFLHGDLVDRLHWLTDRQLLDAVAAGQLTPGPVFSTATFVGYL
                     VAGGRGAAIATIGIFLPAFVFVAVSGPIISRWKDAPAFRATLDAVIAASLGLMGAVTV
                     LLGRTGITSWATLALAAAAALALWRWRLNVAWVLVAGVAIGWLMR"
     gene            1261..1347
                     /locus_tag="VFU45_04570"
     tRNA            1261..1347
                     /locus_tag="VFU45_04570"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1295..1297,aa:Ser,seq:tga)
     gene            1920..2678
                     /locus_tag="VFU45_04575"
     CDS             1920..2678
                     /locus_tag="VFU45_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358370.1"
                     /translation="MPHLLVLKFGGTALGTPPRLRRAVRRLAAHRTAGAEVVAVVSAS
                     GHTTDRILRTLDRVRIGAGATADRETDRALATGEELSAAIVAAGLGALGHPARSFRGG
                     EAGLRTEGPFGGATLVQFDPGPLRRCLAAGVIPVVAGFQGAGQDAETRTLGRGASDLT
                     AVFLAARLGAAECHIVTDVDGVYDSDPRRAADARRHALLSHTALLSLAEDGAEIVHAG
                     AAREALISRVPLQVYSYTAPLRHRTGTWIREGAA"
     gene            2675..3550
                     /locus_tag="VFU45_04580"
     CDS             2675..3550
                     /locus_tag="VFU45_04580"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF006449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU5358371.1"
                     /translation="MISHRIVGPAGAPVVIALGGISAGRNVTRADDGTPGWWEDFVGP
                     GRTIDTNRLRVLGIDWLDAPSPISTQAQADAIAGLLDHLGVARLHAFVGSSYGGMVGL
                     AFAARHPSRLGRLVCISAAHQSHPMATALRALQREAVQLGLATGHADRGMALARGIAM
                     TTYRTVEEFAERFAMEPEPGPAGPRFPVEGYLAEHGRRFIGRFTPERFLSLSLSLDLH
                     RVDPALVSTPTTLVAVAEDTLVPLRQMEELRARLAGPVTLDVIHSRYGHDAFLKEVGP
                     IGLVIKRALGAGSPS"
     gene            3547..4677
                     /locus_tag="VFU45_04585"
     CDS             3547..4677
                     /locus_tag="VFU45_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU5358372.1"
                     /translation="MTRATTRAVRAGLVSDRHHNAVVPPIHLSANYAFPAFGQKGAYD
                     YGRSGNPTRDLLAGAIAELEGGAGAVVTATGMAAVTLVLQLVHGGGRVVAPHDCYGGT
                     HRILSAFARRGLIDVAFVDQRDLTAVERALAVPTRLLWIESPSNPLLRLTNIDAVARL
                     GTAAGALVAADNTFLSPALQRPLELGADIVVHSTTKYLNGHSDVVGGAVVARDTALHQ
                     ELTWWANCLGVSGAPFDSFLTLRGLRTLHVRIRQHVENAQTVAALLLDHPAVGQVHYP
                     GIGAMVSFTLKRGAQAVPAFLSGLVHFTLAESLGGVESLVAHPATMTHASMDAEARAR
                     AGIGDDLLRLSVGIEAVEDLIADVRAALDRTIPLRSRRVAAR"
     gene            4674..>5136
                     /locus_tag="VFU45_04590"
     CDS             4674..>5136
                     /locus_tag="VFU45_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU5358373.1"
                     /translation="MTERIPVAVLGATGLVGQEIVRLLEDHPWFELVAVCASDRSVGR
                     RYGDALRYRSCDPGSATRDLVVSICTPAAIDAPIVFSALDSAVAGEVEEAFAAAGALV
                     VSNARNHRLDDDVPLLIPEVNAAHLALVERQRAGRRWDGAIVTNPNCSTSVI"
BASE COUNT          649 a         1906 c         1739 g          842 t
ORIGIN      
        1 gggcagtgat gctccggggg atcgttcgtc ttcaggcggc ggcgcggccg aggtgctcgg
       61 ggtcttcctc cggctcggcg ctactgcgtt cggcggaccg gcggcccatg tggcgctgat
      121 ggaggaggag ttcgtccgcc ggcgccgatg gctctcccgt accgaactgc tcgacctggt
      181 cgcaagcgcc cagctcatcc ccggccccaa ctccaccgag ctcgccatcc acctcggcta
      241 ccggcgtgcg gggtggcgcg ggctcattgc cgccggcatc ggattcatcc tgccggccgt
      301 gctcctcacc gccctgctcg cgtgggccta cgcccggttc ggggcgctcg tgggagtggc
      361 cggcgcgctc gccggcgcca aggccgtggc cttcgtcgtg gtacttcggg cgctctggga
      421 cttcgggcga tcggcgctca ccacccgctg gcgcatcgcg ctcagcgtcg gcgccctcgc
      481 ggccagcctc atcggcgccg acgaactcgt catcctgggc gtcgcggtcc tcctggggct
      541 cgccaccgtc cgccgaggag ccggggcggc ggcaccggcc ggatatcccc tccttgcccg
      601 cctggccccc gcgctcgccg gcgcacccac gctcccggcc ctccttctca ccttcctcaa
      661 gttcggcgcc gtgctcttcg ggagcgggta tgtgctcctc gccttcctcc acggcgacct
      721 ggtggaccgg ctccactggc tcaccgaccg ccagctcctc gacgcggtgg ccgccggcca
      781 gctcacgccc ggtccggtct tctcgacggc cacgttcgtc ggttatctcg tggccggcgg
      841 gcgcggcgcc gccatcgcga ccatcgggat cttcctgccg gcgttcgtct tcgtcgcggt
      901 gagcggcccg atcatctccc gctggaagga cgcgccggcg ttccgcgcga cactcgatgc
      961 ggtcatcgcc gcctcccttg gcctcatggg ggcggtcacc gtgctcctcg gccgcaccgg
     1021 gatcacgtcg tgggccacgc tggccctcgc cgcagcggcg gcgttggccc tctggcgctg
     1081 gcggctcaac gtggcgtggg tgctggtggc aggcgtggcg atcgggtggc tgatgaggtg
     1141 atctggcagt ccacgcgccc attcacgcgg cggtgcatgc ggaggttcac gcggcagtgc
     1201 acgcggtccc tgacccgctc taccccctcc ccatgccccc gctagattac tccgcgccga
     1261 ggcgtggtgg ccgagtggtt gaaggcaccg gtcttgaaaa ccggcaagcc cgcaagggct
     1321 tcgtgagttc gaatctcacc cacgccgtga gtgtcggtga acagtgaacg gtcaacagtc
     1381 aacgggagag agctgaccct ggagcggcgc ccgccgttct ccatctcccc gttcactttt
     1441 cactcttcac ttttcactct tcaccgtcca cctcttgacc tccggcggag tgttcgctag
     1501 agttggccgc acaactgaag cccgtggtca tttgccgcta ttgcagccac gttaaacaac
     1561 tgctaaaacg cgaccgcctg gcggcagtct tacgccgggc atttcgtttt gtaccagttg
     1621 atggagcgcc gatgcgccca gccgtgctgc acgcaatgga gatgcgaatg accgccgatg
     1681 ccttcgggcc ggacgggagc tcgcgcgcgc gcgcgacata actggcgagg tcttcgctgg
     1741 tccccgaccc cggccctcgt gatgaggatc ggggtcttct gttgcggggt cgtgtcgggc
     1801 atttggggtt catcactggc cttactccaa gccctaagcg ctaagcccta agccctaagc
     1861 gccagaccct aagccctaag cgctaagccc taagcgctgc acctctctcg gagctccaaa
     1921 tgccccacct gctcgtcctc aagttcggtg gcactgcgct cggcacaccg ccgcgcctgc
     1981 gtcgggccgt ccgccgcctc gcggcacatc gcaccgccgg cgccgaggtg gtcgcggtgg
     2041 tgagcgcctc ggggcacacc accgaccgaa tccttcgtac actcgaccga gtaagaattg
     2101 gcgccggcgc caccgccgac cgtgagaccg accgcgccct cgccacgggc gaggagctct
     2161 ccgccgcgat cgtcgccgct gggctcggcg cactcggtca cccggcccgg agcttccgcg
     2221 gtggcgaggc gggcctccgc accgaggggc ccttcggagg cgccacgctg gtgcagttcg
     2281 acccggggcc gctccgccgg tgcctggccg ccggcgtgat cccggtggtg gccggattcc
     2341 agggtgcagg gcaagacgcg gagacccgca cgcttggccg cggcgcatcg gatctgacgg
     2401 cggtgttcct tgccgcccgg cttggcgccg ccgagtgcca tatcgtgacc gacgttgacg
     2461 gcgtgtacga ttcggatccg cgccgggcgg cggatgcgcg gcgtcacgcc ctgctgagcc
     2521 ataccgcgct cctctcactg gccgaggatg gggccgagat cgtgcacgcc ggcgcagcgc
     2581 gggaggcgct catctcgcgg gtgccgctgc aggtctacag ttacaccgcc ccgctccgcc
     2641 atcggacggg tacctggatc agggagggtg ccgcgtgatc tcgcatcgga tcgtgggtcc
     2701 ggccggcgcg ccggtggtca tcgccctcgg cggtatctcg gccggcagga acgtcacccg
     2761 agccgacgat ggcacgcccg gctggtggga ggacttcgtc gggccgggac gcaccatcga
     2821 caccaatcgc ctgcgggtgc tcggcatcga ttggctcgac gcgccgtcgc cgatctccac
     2881 ccaagcacag gccgacgcca tcgccggcct gctcgaccac ctgggcgtgg cgcggcttca
     2941 cgcgttcgtg ggctcgtcgt acggcgggat ggtgggactc gccttcgccg cccgccatcc
     3001 gtcccggctc gggcgcctcg tgtgcatcag cgccgcccac cagagccacc cgatggctac
     3061 tgcgctccgc gcgctccagc gggaggccgt acagttgggc ctcgcgaccg gtcacgcgga
     3121 tcgggggatg gccctcgccc gggggatcgc gatgaccacc taccggacgg tcgaggaatt
     3181 cgccgagcgg ttcgcgatgg agccggagcc cgggcccgca ggaccgcgat tcccggtcga
     3241 ggggtacctg gcggagcacg ggcgacgttt catcgggcgg ttcacgccgg agcgcttcct
     3301 gagcctgtcg ctctcgctcg acctgcatcg cgtcgatccg gcgctggtct ccacgcccac
     3361 gacgctcgtg gcggtagccg aggataccct ggtgccgctc cggcagatgg aggagctgcg
     3421 cgcgcggctt gccgggccgg tgacgctcga cgtcatccat tcccgttacg ggcacgacgc
     3481 gttcctgaag gaggtcggcc ccatcgggct cgtgatcaag cgcgccctcg gcgcggggag
     3541 tccatcgtga cccgcgccac gacccgagcc gtccgtgccg gcctcgtttc cgatcgtcac
     3601 cataatgccg tggtcccgcc gatccacctt tcggccaact acgcctttcc cgcgttcggc
     3661 cagaagggag cctacgacta cggccgcagc ggcaacccga cgcgcgacct cctggccggc
     3721 gccatcgccg agctcgaggg aggggccggc gccgtggtga ccgcgaccgg gatggccgcg
     3781 gtgacgctgg tcctgcaact cgtccacggt ggcggccggg tggtggcgcc ccacgactgt
     3841 tacggcggca cccaccggat cctatcggcg ttcgcgcgcc gcgggctcat cgacgtggcc
     3901 ttcgtggacc aacgggatct caccgcagtc gagcgcgcgc tcgccgtgcc gacccggctc
     3961 ctctggatcg agtcgccgag caaccccctg cttcgactca ccaacatcga cgctgtcgcc
     4021 cggctcggca cggccgccgg cgccctcgtg gcggcggaca acaccttcct gtccccggcg
     4081 ctccagcggc cgctggagct cggtgcggac atcgtggtcc actcgaccac caagtacctg
     4141 aacgggcaca gcgacgtcgt gggcggcgcc gtcgtggcgc gggacacggc gctgcaccag
     4201 gagctgacct ggtgggccaa ctgcctcggc gtgtcgggtg cgccgttcga cagcttcctc
     4261 accctccgcg gcctccggac gctgcacgtc cggatccggc agcacgtcga gaacgcgcag
     4321 accgtcgccg ccctgcttct ggatcacccg gcggtcggcc aggtccacta ccccggcatc
     4381 ggcgcgatgg tgagcttcac cctcaagcgg ggcgcgcagg cggtgccggc gttcctgtcc
     4441 gggctcgtac acttcaccct cgccgagtcg ctcggcgggg tggaatcgct cgtggcccac
     4501 cccgccacga tgacgcacgc ctcgatggac gccgaggccc gggcgcgggc cggcatcggg
     4561 gacgacctgc tccggctctc cgtcgggatc gaggcggtgg aggacctcat tgccgacgtg
     4621 cgggcggcgc tcgaccggac gatcccgctc cgctcccgca gggtggcggc gcggtgaccg
     4681 agcggatccc cgtcgccgtc ctcggcgcga ccggactcgt cggccaggag atcgtgcgcc
     4741 tgctcgagga tcacccctgg ttcgagctcg tggccgtctg cgcgtcggac cggtccgtcg
     4801 gccggcgata cggagacgct ctccggtatc gatcctgtga cccgggatcc gcaacccgcg
     4861 acctcgttgt ctcgatatgc acccctgccg ccatcgacgc gccgatcgtc ttctccgcgc
     4921 tggacagcgc cgtggccggc gaggtcgagg aggcgttcgc ggcggcgggg gcgctcgtcg
     4981 tcagcaatgc gcggaaccat cgcctggacg acgacgtccc ccttctcatc ccggaggtca
     5041 acgccgccca ccttgccctg gtcgagcggc aacgggccgg tcggcgctgg gatggcgcca
     5101 tcgtcaccaa cccgaactgc tccacgtcgg tcatcg
//
LOCUS       DASUAP010000184         5209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_727630, whole genome shotgun sequence.
ACCESSION   DASUAP010000184 DASUAP010000000
VERSION     DASUAP010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727630"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="VFU45_04595"
     CDS             <1..1607
                     /locus_tag="VFU45_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5358374.1"
                     /translation="DRREAARARVDSAARADELGELTEIGIRLSTERDYNKLLETILT
                     QARRITQSDAGSLYVVERDEQGRRRLRFKLSQNHSRPDIPFVEFTIPVDHASIAGYAA
                     AEGEPLVIDDAYTLPPDVEYSFNRSIDQRYGYRTRSMLSVPMLNHHGETIGVLQLINR
                     KRALAATLHSPEDADREVLAYSPHTVKLVKALAGQAGVSIENSQLYESIERLFEGFVK
                     AAVTAIEQRDPTTSGHSLRVATMTVGLAASVDRATDGPYRGVTFSADQIRELRYAGLL
                     HDFGKVGVREQVLVKAKKLYSGDLTLIRQRHAFLQRTAQWRFEQQRADYLERHGRTGY
                     DAHLRSLRAAFEQESQGLDRFLKVVLDSNEPTVLAEGNFEQLQDLAERTYETIDGAVM
                     PWLTDDEVRFLSIRKGSLDEAERLEIESHVTHTYWFLRNIPWTRELQGVPDIAHGHHE
                     KLDGSGYPRHIRGEAIPIQTRMMTIADIFDALTASDRPYKRALPLDRALGIMTDEVKA
                     GMLDPGLFDVFVEAKVYEKRDEGTKG"
     gene            complement(1864..3099)
                     /locus_tag="VFU45_04600"
     CDS             complement(1864..3099)
                     /locus_tag="VFU45_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEU5358375.1"
                     /translation="MRRAAALAAALSVAAPATSAAQATVPGSQLEVSVITVGPGAAVW
                     ERWGHNMIRIRDRQTGLDLVYNYGMFDFAEKGFLLHFLEGRLNYWTAAFYTAPTLEFY
                     RNTSHRSVWEQALRLTPAERLALQQFLDWNGLPQNRVYRYDYYRDNCSTRVRDALDRA
                     LGGAIARATRGRSTGTTDRDHTRRLTQDQPFLRTGLMLLLGPAVDQPLDEWQEMFLPV
                     RMMVHLREVTVDSAGIRLPLVVHEDTLALSDRFPEPVDRGARIPWFLGVGLLLGALLA
                     LGTGRGPGWWWVAGAALVALVLGLGGAVASGLWALTDHTATWRNQNVLQANLLGLVLA
                     VSLRPLAAGRAWARRSAPRLAVVVAVGSVLGLLWHLTIGGQANGDVLALLVPVNVGLA
                     VGVVRVARREATSGEQGGR"
     gene            complement(3096..4106)
                     /locus_tag="VFU45_04605"
     CDS             complement(3096..4106)
                     /locus_tag="VFU45_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5358376.1"
                     /translation="MTAPLRLGIIGAGAVTQVAHLPVLRRMKEVDVVALCDGDLPKAR
                     ALATRFGITNTFDDIEELLEAETLDALLIATPNHLHESHVLAALSAGLHALVEKPLAT
                     SLQGAQRIAKAAQKRDRVVMVGMNHRYRSDVQTVRGFVQSGELGQIDGVQGAWHVFRP
                     SRAQLGWRQRRAESGGGAMLDLGLSVLDLGLWLAGNPTPVRVSATLDRPSGERGVEHS
                     GSAFVVCEEGQSIFVDVTWRHIGEGEYLGVGLRGTKGTAEVNPLRVWKELNGVPVDVS
                     PTGASSRESAFTASVRAEWAHFIAAVAGDAKAPSLEEHLTLHRVLEAIYRSADEGREI
                     AL"
     gene            complement(4128..>5209)
                     /locus_tag="VFU45_04610"
     CDS             complement(4128..>5209)
                     /locus_tag="VFU45_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEU5358377.1"
                     /translation="GVDALRVGAAGSLRSGRTVLVAADGRGLVLAGDDGRTRVAAPLV
                     IAPAERDGLVRVNGRDYRGTVEVRRAGAGAGISVIERVGIESYLAGVVTAELGDHPEG
                     DDQAAMAQAVVSRTYAVRNAGRWRAQGFDLAANIGDQRYDGVQGETAQAWRAVRATRG
                     QILTWQGRPIDAFFHSTCGGRTADAAEVFSGGRRPYLRSIADVDGDGLPYCRISPRYQ
                     WREDWTAAELVSALRRTLPAESGVRASDVTVVRGIEVAGRTVSDRVARLVIRLGTRTV
                     TVEGAAIRRVLVPPGQEILRSAAFTLRPLTTNGRLAALVAEGRGAGHGVGLCQWGAIG
                     RARAGQRYPQILAAYFPGASLTRQF"
BASE COUNT          816 a         1965 c         1756 g          672 t
ORIGIN      
        1 tcgaccggcg cgaggcggcg cgcgcccggg tcgacagcgc cgcgagggcc gacgaactcg
       61 gcgagctgac cgagatcggc atccggctca gcaccgagcg agactacaac aagctgctcg
      121 agacgatcct gacccaggcc cgacggatca cccagtcgga cgccggcagc ctctacgtcg
      181 tcgagcgcga cgagcagggc cgccgccggc tccgcttcaa gctgagccag aaccacagcc
      241 ggcccgacat cccgttcgtc gagttcacca tcccggtcga ccacgcgtcg atcgcggggt
      301 acgctgccgc cgagggggag ccgctcgtca tcgacgacgc gtacaccctg ccccctgacg
      361 tcgagtattc gttcaaccgc tcgatcgacc agcgctacgg ctatcgcacc cgctcgatgc
      421 tgagcgtgcc gatgctcaac caccacggcg agaccatcgg cgtcctccag ctcatcaacc
      481 gcaagcgcgc gctcgccgcg acgctccact cgccggagga cgccgaccgc gaggtgctgg
      541 cgtactcccc ccacacggtg aagctggtca aggcgctcgc cggccaggcg ggcgtctcga
      601 tcgagaacag ccagctctac gagtcgatcg agcgcctctt cgaggggttc gtgaaggcgg
      661 cggtgaccgc catcgagcag cgggacccga cgacgtccgg ccactccctc cgcgtcgcca
      721 cgatgaccgt agggctcgcc gcctccgtcg accgcgcgac cgatggcccc taccggggcg
      781 tcaccttctc ggccgaccag atccgggaac tgcgctacgc gggcctcctg cacgacttcg
      841 gcaaggtcgg cgtgcgcgag caggtgctgg tcaaggccaa gaagctctat tccggcgacc
      901 tgaccctcat ccgccagcgc cacgccttcc tccagcggac cgcccagtgg cgcttcgagc
      961 agcagcgcgc cgactacctc gaacgccacg gccgcaccgg ctacgacgcg cacctgcggt
     1021 ccctccgcgc cgcgttcgag caggagagcc agggcctcga ccgcttcctc aaggtggtgc
     1081 tcgactcgaa cgaaccgacg gtgctggcgg aggggaactt cgagcagctg caggacctgg
     1141 cggagcggac ctacgagacc atcgacggtg cggtcatgcc ctggctcacc gacgacgaag
     1201 tgcggttcct gagcatccgg aaagggagcc tcgacgaggc ggagcggctc gagatcgaga
     1261 gccacgtgac ccacacctac tggttcctgc ggaacatccc ctggacccgg gaactccagg
     1321 gggtgccgga catcgcccac gggcaccacg agaagctcga cggctccggc tacccgcgcc
     1381 acatccgcgg cgaggccatc ccgatccaga cccggatgat gaccatcgcc gacatcttcg
     1441 acgcgctcac cgcctcggac cgcccataca agcgcgccct cccgctcgac cgcgccctcg
     1501 gcatcatgac cgacgaggtg aaggccggga tgctcgatcc ggggctcttc gatgtgttcg
     1561 tggaggccaa ggtgtatgag aagagggacg aggggacgaa gggatgaggg aaagagagga
     1621 agaggggaag cgcggaagag gcgacgagga gaagagggga cgaccggacg gggagatgag
     1681 gtgacggcgt aggtccacga aatatgagtg gtgagcgcca ccggtcgact ggactcacca
     1741 acgccgggca tatgggcgtc gcttcacctc atccccgctt cccctcccct cctccacctc
     1801 acgtcatacc atctccgcct cttctcctct tcttctcctc ttctcctctt ctccctctcc
     1861 ttctcacctc ccgccctgct caccgctggt cgcctcccgg cgcgccaccc gtaccactcc
     1921 caccgccagc cccacgttga ccggcaccag cagcgcgagc acgtcaccgt tggcctgccc
     1981 gccgatggtc aggtgccaga ggagccccag gaccgagccg accgcgacga ccaccgccag
     2041 gcgcggcgcc gaccgacggg cccaggcccg ccctgccgcg agcggccgga gcgatactgc
     2101 cagcaccagg ccgagcaggt tggcctgcag gacgttctgg ttccgccagg tggcggtgtg
     2161 gtcggtaagg gcccagagcc ccgacgccac ggccccgccg agccccagca ccagcgccac
     2221 cagcgccgcc ccggccaccc accaccaccc cggcccgcgt ccggtgccga gcgcgaggag
     2281 cgcgccgagc aggaggccga cgcccaggaa ccaggggatc cgcgccccgc ggtccaccgg
     2341 ctcgggaaag cggtcgctca gcgcgagcgt gtcctcatgc accaccagcg ggagccggat
     2401 gccggcggag tcgacggtca cctcgcgcag gtgcaccatc atccgcacgg gcaggaacat
     2461 ctcctgccac tcgtcgagcg gctggtccac cgcgggcccg aggagcagca tcaggccggt
     2521 gcgaaggaag ggctggtcct gggtcagccg gcgggtgtgg tcgcggtcgg tggtgccggt
     2581 cgaccgcccg cgcgtcgcgc gggcaatggc gccgcccaac gcccggtcca gcgcgtcgcg
     2641 gacccgggtg gagcagttgt cccgatagta gtcgtagcgg tagacccggt tctggggcag
     2701 cccattccag tccaggaact gctggagggc aagccgctcg gccggcgtga gccgcagcgc
     2761 ctgctcccac accgagcgat ggctggtgtt ccggtagaac tcgagcgtcg gcgcggtgta
     2821 gaacgcggcg gtccagtagt tgagccggcc ctcgaggaaa tggagcagga atcccttctc
     2881 cgcaaaatcg aacatcccgt agttgtacac gaggtcgagg ccggtctggc ggtcgcggat
     2941 gcggatcatg ttgtgccccc accgctccca cacggccgcc ccgggcccga ccgtgatgac
     3001 cgacacctcg agctgcgacc cggggacggt cgcctgggcc gccgacgtcg ccggggcggc
     3061 gaccgagagc gccgcggcca gtgccgcggc acgcctcaca gggcgatctc ccggccctca
     3121 tcggcggagc ggtagatcgc ctcgagcacc cggtggagcg tcaggtgctc ctccaggctc
     3181 ggcgccttgg cgtcgcccgc caccgctgcg atgaaatggg cccattcggc gcgcaccgag
     3241 gccgtgaacg cactctcccg gctcgacgcc ccggtgggcg agacgtcgac cggcacgcca
     3301 ttcagctcct tccacacgcg gagcggattg acctccgccg tgcccttggt gccgcgcaac
     3361 ccgaccccca ggtactctcc ctccccgatg tggcgccagg tcacgtccac gaagatcgac
     3421 tggccctcct cgcacaccac gaacgcgctc cccgaatgct ccacgccgcg ttccccgctc
     3481 ggacggtcga gggtggcgct cacccgcacc ggcgtcggat tgccggcgag ccagagcccg
     3541 aggtcgagca ccgagagccc gagatcgagc atcgcgccgc cgcccgactc ggcccggcgc
     3601 tggcgccagc cgagctgggc ccggctcggc cggaacacgt gccatgcccc ctgtacgccg
     3661 tcgatctggc ccagttcccc gctctgcacg aacccgcgga cggtctggac gtccgagcgg
     3721 taacggtggt tcatcccgac catcaccacg cggtcgcgct tctgggccgc cttcgcgatg
     3781 cgctgggccc cctgcagcga ggtggccagc ggcttctcca ccagggcgtg gaggcccgcc
     3841 gacagcgcgg cgagcacgtg gctttcatgc aggtggttcg gggtggcaat gagcagcgcg
     3901 tcgagggtct ccgcctccag cagctcctcg atgtcgtcga acgtgttggt gatcccgaac
     3961 cgcgtcgcaa gggcccgggc cttggggagg tcgccgtcgc agagcgccac gacatcgacc
     4021 tccttcatgc ggcggaggac cggcaggtgg gccacctggg tcacggcgcc agcgccgatg
     4081 atgccgagcc ggaggggagc ggtcatggga tggtatccct gggtgggtca gaactgtcgg
     4141 gtgagcgaag cgccggggaa atatgcggcg aggatctgcg gatagcgctg ccccgcccgg
     4201 gcacggccga tggcgcccca ctggcagagc ccgacgccgt ggccggcgcc ccgcccctcc
     4261 gccacgagcg cggccaggcg cccgttggtg gtgagcgggc ggagggtgaa ggccgcgctc
     4321 cgcaggatct cctgccccgg gggcaccagg acccgccgga tcgccgcgcc ctcgaccgtc
     4381 accgtgcggg tgccgagccg gatgacgagc cgcgccaccc ggtcggagac cgtgcgcccc
     4441 gccacctcga tcccccgcac tacggtcacg tcggacgcgc ggacaccgct ctccgccggg
     4501 agggtgcgcc ggagcgccga cacgagctcg gcggcggtcc aatcctcgcg ccactggtag
     4561 cgcggcgaga tgcggcagta cgggaggccg tccccgtcca catcggcgat ggaccggagg
     4621 tacgggcggc gtccgccgga aaagacctcc gccgcgtcgg cggtgcgccc gccgcaggtc
     4681 gagtggaaga aggcgtcgat gggacgcccc tgccaggtca ggatctggcc ccgggtagcg
     4741 cgcaccgccc gccatgcctg cgccgtctcc ccctggaccc cgtcgtagcg ttggtcgccg
     4801 atgttggccg cgaggtcgaa gccctgcgcc cgccaccgtc ccgcgttgcg caccgcatac
     4861 gtgcgcgaca ccaccgcctg cgccatggcg gcctggtcat cgccctcggg atggtcaccc
     4921 agctcggcgg tgaccacacc ggccaggtac gactcgatac ccacccgctc gatcaccgag
     4981 atgccggcgc cggccccggc gcggcggacc tcgacggtac cgcgatagtc ccggccgttc
     5041 acccggacga ggccgtcccg ctccgccggc gcgatgacga gcggcgccgc cacccgcgtc
     5101 cggccgtcgt ccccggccag gacgagcccg cggccgtccg cggccacgag caccgtccgg
     5161 ccggagcgga gcgaccccgc ggcaccgacc cggagtgcgt cgacgccgc
//
LOCUS       DASUAP010000185         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_986592, whole genome shotgun sequence.
ACCESSION   DASUAP010000185 DASUAP010000000
VERSION     DASUAP010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986592"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFU45_04615"
     CDS             complement(<1..407)
                     /locus_tag="VFU45_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358378.1"
                     /translation="MPSRDANGARSIAVEFSLLIGGLLIVGASIGAVVTYSSVRDASM
                     SLSVQRLASVTAQLRDMLDANGAQYRRTMRAVADTPAVRAFIRTPDAARRAEVARILA
                     PSADIADQVATREIRSASDSLLLAVGDVQRWADP"
     gene            complement(568..1281)
                     /locus_tag="VFU45_04620"
     CDS             complement(568..1281)
                     /locus_tag="VFU45_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358379.1"
                     /translation="MRCPRPARILPLLLLLAPAMVHAQSADDIVARSITARGGLARIK
                     AVTTTRLTGTIAFGSAAPGLFRVTMERGGKSREEVETPGGMLVRWTDGVHGWIRAPGD
                     SVTRELTAAELQNMAGSTDMDGPFVDAAAKGNRIELIGKDTAEGRPAWKLRVTGRDSS
                     VRYAWFDADTWLQLKWEGIIHGNGRDFDVVSLFSDYRPEHGIEFARAIDSGPQRIRFT
                     AIEVDVPVPDTEFAAPTGD"
     gene            complement(1350..>2838)
                     /locus_tag="VFU45_04625"
     CDS             complement(1350..>2838)
                     /locus_tag="VFU45_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5358380.1"
                     /translation="RRHRDLTESAPLGICQSTVDGTFIMANHELARILGYDSRAELTQ
                     RALPELYADPAERAALVSEALRTLRPHDAIVTWKRKDGTPIRVQLTVRAVLGADAAVQ
                     HIEGFVRDVTQQERLEEQYRQAQKLEAVGRLAGGVAHDFNNILTAIIGSCDLALARTR
                     EGDSPVEELEEIQRAADRAAGLTRQLLAFSRKSVVAPRPLDLNEVIRSLGEMVRRLIG
                     EDILLTFALEDGIGTVNADPGEIHQVLLNLVVNARDAMPRGGRLTIATSAVRIDDAAS
                     GLPTGAYVRLAVTDTGTGMSEEVKRHIFEPFFTTKESGQGTGLGLATVYGIVQRSGGH
                     VSVYSEEGRGASFRIHLPRSDAPAETAAPAAEAAARGGSETLLVVEDADAVRKVTCRI
                     LRNRGYRVLEAAHGREALAAVATTDRPIDLLVTDVVMPEMSGRELAERLQAERPGLKV
                     LYLSGYTDDAVVRHGILEATVAFLEKPYAAEALAQKVRQVLDRPA"
BASE COUNT          381 a          984 c         1033 g          440 t
ORIGIN      
        1 gggtcggccc agcgctggac gtcgcccacg gccaggagca gcgaatccga ggcggaacgg
       61 atctcccgcg tggcgacctg gtccgcgatg tcggccgagg gcgccaggat ccgggccacc
      121 tcggcgcggc gcgccgcgtc aggcgtccgg ataaatgccc gcacggccgg cgtgtccgcc
      181 accgcgcgca tggtccggcg atactgggcg ccgttggcgt cgagcatgtc gcggagctgg
      241 gcggtgacgc tcgccaggcg ctggacactg agcgacatcg acgcgtcgcg gaccgaactg
      301 taggtcacca cggcgccaat ggacgcgccg acgatcagga gcccgccgat caggagagag
      361 aactcgaccg cgatcgagcg ggcgccattg gcatcgcgag atggcatcgg cggatcaccg
      421 tgcatgaggg gcgggggagt cctgcgttcc aatactgcgc cggcggcccc gccgcgcaac
      481 cggcaaaagc cccggaacgg tgtatcgaat tgcgacggtc ggcgaacgga ggggacaacg
      541 tccctccggg gtcgccgatc cgcgccgtca gtcacccgtg ggagcggcga actctgtgtc
      601 gggtaccgga acgtcgacct cgatcgccgt gaacctgatc ctctgtggcc cggagtcgat
      661 cgcccgcgcg aattcgatcc cgtgctccgg ccggtagtcg ctgaagagcg acaccacgtc
      721 gaagtcccgt ccgttgccgt ggatgatccc ctcccacttg agttgcagcc aggtgtcggc
      781 gtcgaaccag gcgtagcgca ccgagctgtc gcggccggtg accctgagct tccacgccgg
      841 gcgcccctca gcggtgtcct tgccgatgag ctcgatccgg ttccccttgg cggcggcgtc
      901 gacgaacggg ccgtccatgt cggtgcttcc cgccatgttc tgcagctcgg ccgcggtgag
      961 ctcgcgggtc acggagtctc cgggcgcgcg gatccatccg tgcaccccgt cggtccagcg
     1021 gaccagcatc ccaccgggcg tctccacttc ctcacgtgac ttcccgcccc gttccatcgt
     1081 gacgcggaag agcccgggcg ccgcggagcc gaatgcgatg gtgccggtga ggcgggtggt
     1141 ggtgaccgcc ttgatccgtg cgagcccgcc gcgcgccgtg atggaccggg cgacgatgtc
     1201 gtcggccgac tgcgcgtgga ccatggcggg cgccaggagg agcaggagcg ggaggatacg
     1261 ggcaggacgt gggcagcgca tcggggtccc tggtgaatgg atggcgtgag gacgatcggc
     1321 cgccctgggc ctgcacatcc gcccgggggt cacgccggcc ggtccagcac ctggcggacc
     1381 ttctgcgcca gcgcctccgc ggcgtagggc ttctccagga acgccacggt ggcctcgagg
     1441 atgccgtggc gtaccactgc gtcgtcggtg tatcccgaga ggtacagcac cttgagcccg
     1501 gggcgttccg cctgcaggcg ttcggccagt tcgcggccgc tcatctccgg catcaccaca
     1561 tccgtgacca gaaggtcgat cgggcggtcc gttgtggcca cggccgccag cgcctcgcgg
     1621 ccgtgggcgg cctcgagcac gcggtagccg cggttgcgga ggatgcggca ggtcaccttg
     1681 cgcaccgcgt cggcgtcctc gacgaccagc agggtctcgc tgcctccgcg ggccgcagcc
     1741 tccgccgcgg gtgccgcggt ctcggccggc gcgtcgctcc ggggaaggtg gatccggaag
     1801 ctggctccgc gtccctcctc gctgtatacg gacacgtggc cgccgctccg ttgcacgatg
     1861 ccgtacaccg tggcgaggcc gaggccggtg ccctggccgc tctccttggt ggtgaagaac
     1921 ggctcgaaga tgtggcgctt gacctcctcg gacatcccgg tgccggtgtc cgtcaccgcg
     1981 aggcgcacgt atgcgcccgt cgggaggccg ctggcggcgt cgtcgatccg cactgccgac
     2041 gtggcgatcg tcagccggcc gccgcgcggc atcgcatcgc gcgcgttgac caccaggttg
     2101 aggagcacct ggtgtatctc gccggggtcg gcgttcacgg tgccgatccc gtcctcgagc
     2161 gcgaaggtga gcaggatgtc ctcgccgatc agtcgccgga ccatctcccc caggctgcgg
     2221 atgacctcgt tcaggtccag ggggcggggc gccaccaccg acttccggct gaaagccagg
     2281 agctggcgcg tgaggccggc ggcccggtcc gcggcccgct ggatctcctc cagctcttcg
     2341 accggcgagt caccttcccg cgtgcgcgcg agcgccaggt cgcaggagcc gatgatggcg
     2401 gtcaggatgt tgttgaagtc gtgggcgacg ccgccggcca ggcgtccgac cgcctcgagc
     2461 ttctgcgcct ggcggtactg ctcctccagc cgctcctgct gggtgacgtc ccgcacgaag
     2521 ccttcgatgt gctggacggc ggcgtcggcg ccgagcactg cgcgcaccgt gagctgtacc
     2581 cggatgggtg tgccgtcctt ccgcttccag gtcacgatgg cgtcgtgcgg gcgcagggtc
     2641 cggagcgcct cgctgacgag cgccgcgcgc tccgcggggt cggcatagag ctcgggcagt
     2701 gcgcgctggg tcagttcggc ccgcgagtcg taccccagga tccgcgccag ttcgtggttg
     2761 gccatgatga acgtgccgtc caccgtcgac tggcagatgc cgagcggggc cgattcggtc
     2821 aggtcgcgat gccggcga
//
LOCUS       DASUAP010000186         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_605006, whole genome shotgun sequence.
ACCESSION   DASUAP010000186 DASUAP010000000
VERSION     DASUAP010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605006"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFU45_04630"
     CDS             complement(<1..332)
                     /locus_tag="VFU45_04630"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEU5358381.1"
                     /translation="MTKVLTLASQSPRRKQLLEMLGLPILVQPSDVYEKPLPNEEPLA
                     YARRLAREKAQAGTGEYVLGADTIVVLDQHLLEKPVDDADALRMLRLLQGRTHEVITS
                     IALLAGGKL"
     gene            complement(329..772)
                     /gene="dtd"
                     /locus_tag="VFU45_04635"
     CDS             complement(329..772)
                     /gene="dtd"
                     /locus_tag="VFU45_04635"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEU5358382.1"
                     /translation="MRVVLQRVSKAAVTVDGRITGAIGRGYCLLVGYTAGDTEAANAW
                     MAEKVSGLRLFGDAEGKMNLGPAEVEGGLLVVSQFTLYGDASKGRRPSFVDAARPEVA
                     EPLYRDFVAKLRATGLPVETGEFGAMMQVEIHNDGPVTLILERTA"
     gene            838..1725
                     /locus_tag="VFU45_04640"
     CDS             838..1725
                     /locus_tag="VFU45_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5358383.1"
                     /translation="MTLTKVRNLFEAARRTSRIRKEMAELDRGVSLRSAPRRPDPAAW
                     PDTGIHAAWLGHATVLLKVEGKLILTDPVLSRKVGIRVAGHTVGPRRHVLPALRVEEL
                     PRPDLLLLSHAHFDHLDLPTLRRIPRDVPVVSAHGLGDLLKRFHHRTELKWGMTAEVA
                     GIRVGALEVKHWGARMVTDRHRGYAGFLLEHGRRRVVFAGDTAMTDAFRKVSGPVHLA
                     LMPIGAYDPWIANHADPEEAWAMAGMMGAERVLAIHHQTFRLSREPMDEPIRRFLAVA
                     GSEAWRVSGREVGETVSIG"
     gene            complement(1896..>2314)
                     /locus_tag="VFU45_04645"
     CDS             complement(1896..>2314)
                     /locus_tag="VFU45_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358384.1"
                     /translation="WTYPQFEMGYAYHFFEPLFDERHSLAYGGGENGMTELVFCPLSK
                     KQAEVEGTLVISDDSGLARATWHFVVPRDDERAGGEATFAPLRPAAPPGLLMTVRSEF
                     WRHTSRGRWYQRAWTFGDWSGDSAEARRAASRQIQR"
BASE COUNT          365 a          809 c          780 g          360 t
ORIGIN      
        1 agctttcccc ccgccagcag cgcgatcgac gtgatgacct cgtgggtgcg cccctgcagg
       61 agccggagca ttcgaagggc atcagcgtcg tcgacgggct tttccagcag atgctggtcg
      121 aggacgacga tcgtgtcggc acccagcacg tattcgccgg tgcccgcctg ggccttttcc
      181 cgcgccaggc gccgggcgta ggccagcggt tcctcgttcg gaagcggctt ctcgtacacg
      241 tcgctcggct gcaccaggat cgggagtccg agcatctcga ggagctgctt gcggcgggga
      301 gactgggagg cgagggtgag caccttggtc acgcggtcct ctcgaggatc agggtcacgg
      361 ggccatcgtt atggatctcc acctgcatca tcgcgccgaa ttcacccgtc tccaccggga
      421 ggccggtggc ccgcagcttg gcgacgaagt cccggtagag cggctcggcc acctcgggac
      481 gcgccgcgtc cacgaacgac gggcgccgtc ccttcgatgc gtccccgtac agcgtgaatt
      541 gcgacaccac caggagcccg ccttccacct cggccggccc caggttcatc tttccctcgg
      601 cgtcgccgaa gagccggagc ccggagacct tctccgccat ccaggcgttc gcggcctcgg
      661 tgtcgccggc ggtgtagccg acgagcaggc agtacccccg gccgatcgcg ccggtgatgc
      721 gcccgtcgac cgtcaccgcc gccttcgaca cccgttgcag caccacccgc atcgcgcccc
      781 cgtggcctgg accgacatga tggacgggaa caagatagcc acaccggaaa gaccccgatg
      841 acgctcacca aggtccgcaa cctcttcgag gccgcgcgcc gcacgtcgag gatccggaag
      901 gagatggccg agctcgaccg cggcgtgtcc ctccggtcgg cgccccggcg ccccgatccc
      961 gccgcctggc ccgacacggg gatccacgcc gcctggctgg ggcacgccac cgtgctcctc
     1021 aaggtcgaag ggaagctgat ccttaccgac ccggtcctga gccggaaggt cgggatccgc
     1081 gtggcgggcc acaccgtcgg cccccggcgg cacgtgcttc ccgcgctcag ggtggaggag
     1141 cttccccgcc ccgacctgct gctcctctcc cacgcgcact tcgaccacct ggacctcccc
     1201 accctgcgcc gcatcccgcg cgacgttccc gtggtgtcgg cccacggcct gggcgacctg
     1261 ctcaagcggt tccatcaccg caccgagctc aagtggggga tgacggcgga ggtggcgggc
     1321 atccgggtcg gggcccttga ggtgaagcac tggggggcgc ggatggtcac cgaccggcac
     1381 cgtggctacg ccggcttcct gctggagcac gggcggcgcc gggtggtctt tgccggcgac
     1441 acggcgatga ccgacgcctt ccggaaggtg tccggccccg tgcacctggc gctgatgccg
     1501 atcggcgcct acgacccgtg gatcgccaac cacgccgacc cggaggaggc gtgggcgatg
     1561 gccgggatga tgggtgccga gcgggtgctg gccatccacc accagacgtt ccggctgagc
     1621 cgggagccga tggacgagcc gatccggcgt ttcctggcgg tggcagggag cgaggcgtgg
     1681 cgggtgagcg gacgggaggt gggggagacg gtgtcgatcg ggtgataagt aagaagtgag
     1741 aagtgagaag gagggaagag ggaaaaggga atagagggaa aggaaggaga aggaggagaa
     1801 aggaccgggc tccctctctc ctccgtccca ctctctctcc acctttcact tgtccctttt
     1861 ccctcttccc tatctgcttc gtctcctccg cgccttcaac gctgtatctg gcgggatgcc
     1921 gcgcgccgag cctcggcgga atccccgctc cagtcgccga atgtccacgc cctctggtac
     1981 caccgaccgc gggacgtgtg gcgccagaac tcgctccgta ccgtcatcag gaggccgggt
     2041 ggcgccgcgg ggcgtagcgg cgcgaacgta gcctccccgc cggcccgctc gtcatcccgc
     2101 gggaccacga aatgccaggt cgctcgggcg agacccgaat cgtcggaaat gaccagcgtg
     2161 ccttccacct ccgcctgctt cttcgagagg gggcagaaga ccagctccgt cattccgttc
     2221 tcgccgcccc catatgcgag cgagtgccgt tcatcgaaga gcggctcgaa gaaatggtag
     2281 gcgtatccca tttcgaactg cggataggtc cagg
//
LOCUS       DASUAP010000187         3691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_264479, whole genome shotgun sequence.
ACCESSION   DASUAP010000187 DASUAP010000000
VERSION     DASUAP010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264479"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFU45_04650"
     CDS             <1..504
                     /locus_tag="VFU45_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU5358385.1"
                     /translation="YAVELAMMAMARRAGADPEAWGVVGLLHDFDYERFPNDAHSATA
                     EHPAEGVRILATMGAPEEWQQAILGHAGYTGVPRTSPMAKALFAVDELCGFLVACALV
                     RPARSLKDLEVSSVKKKLKDKGFAKGVSRDDITVGAAELGMPLEELIAFVLEALRPEE
                     QRLGLGT"
     gene            528..1937
                     /locus_tag="VFU45_04655"
     CDS             528..1937
                     /locus_tag="VFU45_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU5358386.1"
                     /translation="MPAPVQPDPLVTRALDRAAGSRAIPGNRVDLLLDGPVAYPVMLD
                     LIARARRWIHFENYIIHDDPTGWRFAEALAARARAGVRVRVLADWLGSRATGRRFWGF
                     LRAAGVEVRLFGRPSWDLFRNFMRDHRKIVAIDGTEAVTGGLCIGDEWAGRPDRGVLP
                     WRDTAVRIAGPAVPALDLAFARTWAAAGGVLPPAEALPSPEEQGDAIVRVIAVEPGQA
                     RSYRTIQLLTAGAADQLWITDAYLVPPPPLLQGLLDAAHDGTDVRLLVPGASDLPLVR
                     NLTRTGYRDLLAGGVRILEWDGPMLHAKSIVVDGRFGRVGSSNLNASSLLGNYELDVV
                     IEDREFCVALEVQFRIDAARSSEVVLRPRRAPERLQPYLPPAFDRVPGPDSAEHPPLA
                     PHLRTPKERRGRTVVALRTVASAARRSIYGPLLVAFALLAGIAIALPRVVSWSVGILS
                     LYLSAAAAAELFRRRRREG"
     gene            1981..2523
                     /locus_tag="VFU45_04660"
     CDS             1981..2523
                     /locus_tag="VFU45_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5358387.1"
                     /translation="MTDADLAARAARGDADAFGELLERYAPAARRLARAALGNHDDGD
                     DAAQDGFLAAWQNFDKYDAARPFGPWLLRIVVNRARDLGRRRKVRQTDELPPDRPAEI
                     ASPERETDRSLLRAQLAKALDRLPERQRIAITLFDGEGYPHAEIARMLDVPEGTVRSD
                     VFHARRTLREALGQWQGGTA"
     gene            2520..2837
                     /locus_tag="VFU45_04665"
     CDS             2520..2837
                     /locus_tag="VFU45_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358388.1"
                     /translation="MTDNPFDAGPDEALGALLRETLSRGDDAAFTARVRQALPAAQPG
                     AWDLLARWARPGLVAAALVALAAGLWLSQGNGNGTTSSTDLFASADQTPPDVILTLSM
                     EGR"
     gene            2834..3226
                     /locus_tag="VFU45_04670"
     CDS             2834..3226
                     /locus_tag="VFU45_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEU5358389.1"
                     /translation="MTGTEVPRRATSRLGATLLLIAVFVAGAIAGGALEATHVLGPRR
                     PARGPDGFVRHLSDELDLSAAQQDSVRAILQRHRAQMDSAWAEIRPRFETLRQVIRSE
                     IAAQLDGQQREKFDDLNRREEARRPDHH"
     gene            3228..>3691
                     /locus_tag="VFU45_04675"
     CDS             3228..>3691
                     /locus_tag="VFU45_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5358390.1"
                     /translation="MTYRRSLLVPALVAALAAPASAQQRSVSLSEAITMAQRVQPATV
                     QAQGAIRSADAQKLAAYGEFMPSVNYTASASTAHNDLPSYTDQNGQVHSKSSTSNTFS
                     NGISASIDLFTGLRRGADLSAATASQRAADAGLVNARYQVALTTTQSFITALT"
BASE COUNT          498 a         1427 c         1267 g          499 t
ORIGIN      
        1 tacgcggtcg agttggccat gatggcgatg gcccggcggg ccggcgccga cccggaggcg
       61 tggggcgtgg tcgggctcct ccacgacttc gactacgagc gcttcccgaa cgacgcgcat
      121 tccgccaccg ccgagcaccc cgcggaaggg gtccggatcc tcgccacgat gggggcgccg
      181 gaggaatggc agcaggccat cctgggccac gccggctaca ccggcgtccc ccgcacctcc
      241 ccgatggcca aggcgctctt cgcggtcgac gagctctgcg gtttcctggt cgcgtgtgcg
      301 ctcgtccgtc cggcgcggag cctcaaggac ctggaggtgt cgagcgtcaa gaagaagctc
      361 aaggacaagg gcttcgccaa gggcgtcagc cgcgacgaca tcaccgtcgg cgccgccgag
      421 ctgggaatgc cgctcgagga gctgatcgcg ttcgtgctcg aggcgttgcg tccggaggag
      481 cagcgcctgg ggctcggcac ctagggccgt tccaaccccg cccccggatg cccgcccccg
      541 tgcagcccga cccgctggtc acccgcgccc tcgaccgggc cgccgggtca cgcgccatcc
      601 ccggcaaccg cgtcgacctg ctgctcgacg ggccggtggc gtacccggtg atgctcgacc
      661 tcatcgcccg cgcgcggcgg tggatccact tcgagaatta catcatccac gacgacccga
      721 ccggctggcg cttcgccgag gcgctcgcgg cgcgggcacg ggccggcgtc cgggtccggg
      781 tgctcgccga ttggctcggg tcgcgcgcga cggggcggcg gttctggggc ttcctccgcg
      841 cggccggcgt cgaggtgcgc ctcttcggcc ggccgagctg ggacctgttc cgcaacttca
      901 tgcgcgacca ccgcaagatc gtggcgatcg acgggacgga ggcggtgacc ggcggcctct
      961 gcatcggcga cgaatgggcc gggcgccccg atcgcggcgt cctcccctgg cgcgacaccg
     1021 ccgtgcgcat cgcggggccc gcggtgcccg cgctcgacct cgccttcgcg cggacctggg
     1081 cggccgcggg cggcgtcctc ccccccgccg aagcgctccc ctcccccgag gagcagggcg
     1141 acgccatcgt ccgggtcatc gcggtcgagc cggggcaggc caggtcgtac cggaccatcc
     1201 agctcctcac cgccggcgcg gccgaccagc tctggatcac cgacgcctat ctggtgccgc
     1261 caccaccgct gctgcagggg ctccttgacg ccgcgcacga cgggaccgat gtccggctcc
     1321 tcgtgcccgg tgccagcgac ctgccgctgg tgcggaacct gacccgcacc ggctaccgcg
     1381 acctcctggc cggcggcgtc cggatcctgg agtgggacgg cccgatgctc cacgccaaga
     1441 gcatcgtggt cgacgggcgg ttcggccggg tgggcagcag caacctcaat gcgtcgagcc
     1501 tcctcggcaa ttacgaactg gacgtcgtga tcgaggaccg cgaattctgc gtggcgctcg
     1561 aggtccagtt ccgcatcgac gccgcccgga gctctgaggt cgttctccgg ccccgccgcg
     1621 cccccgagcg tctccagccg tatctccccc ccgccttcga ccgggtcccc gggcccgaca
     1681 gcgcggaaca cccgccgctc gccccgcacc tgcgcacacc gaaggagcga cggggccgga
     1741 cggtcgtggc gctccggacc gtcgcgagcg ccgcccggcg ttcgatctac ggcccgttgc
     1801 tggtggcgtt cgcgctgctg gccggcatcg cgatcgcgct gccgcgcgtc gtctcgtgga
     1861 gcgtgggcat cctttcctta tatctctcgg cggccgccgc ggcggagctc ttccgccgcc
     1921 gccgccggga gggctgaccc cgacccccaa cggatgcccc ccaccccgcg tactacgacg
     1981 gtgaccgacg ccgacctcgc tgcccgcgcc gcccggggag atgccgacgc cttcggcgag
     2041 ttgcttgaac ggtacgcccc ggcggcgcgc cgcctggccc gggcggccct gggcaaccac
     2101 gacgacggcg acgacgcggc gcaggacggg ttcctggcgg cctggcagaa cttcgacaag
     2161 tacgatgcgg cgcggccctt cggcccctgg ctcctccgga tcgtggtcaa ccgggcgcgc
     2221 gacctcggcc gccggcggaa ggtgcggcag accgacgaac tcccgcccga ccgtcccgcc
     2281 gagatcgcaa gcccggagcg cgagaccgac cggtcgctcc tgcgggcgca gctggcgaag
     2341 gcgctggacc gcctccccga gcggcagcgg atcgcgatca cgctcttcga cggcgagggc
     2401 tatccccacg ccgagatcgc caggatgctc gatgtgccgg aagggaccgt gcggtcggac
     2461 gtcttccacg cgcggcggac gctgcgcgag gccctgggcc agtggcaagg aggaacagca
     2521 tgaccgacaa cccgttcgac gccggccccg atgaggcgct cggcgcgctg ctccgcgaga
     2581 ccctctcgcg gggcgatgac gccgcgttca cggcacgggt ccgccaggcg ctcccggcgg
     2641 cgcagccggg ggcgtgggac ctcctggccc gctgggcgcg ccccggcctc gtggcggcgg
     2701 cgctggtagc gctggccgcc ggcctctggc tgtcgcaggg gaacggcaac gggaccacgt
     2761 cctcgaccga cctcttcgcc tcggccgacc agacgccgcc cgacgtgatc ctcacgctca
     2821 gcatggaggg ccgatgaccg gcaccgaggt cccccgccgg gcgacgtccc gcctgggcgc
     2881 gaccctgctc ctgatcgcgg tgttcgtcgc cggtgccatc gcgggcggcg cactcgaggc
     2941 cacccatgtg ctggggccgc ggcgcccggc gcgcggtccc gacggcttcg tacgccacct
     3001 gtccgatgaa ctggacctga gcgcggccca gcaggacagc gtgcgcgcca tcctccagcg
     3061 gcaccgcgcc cagatggatt cggcgtgggc ggagatccgt ccgcgctttg aaactttgcg
     3121 gcaggtcatc cgttcggaga tcgccgcgca actcgacggc cagcagcgcg agaagttcga
     3181 cgacctgaac cgccgtgaag aagcccggcg cccggaccat cactgacgtg acataccgca
     3241 gatccctgct cgtcccggcg ctcgtcgccg ccctcgccgc tcccgcgtcc gcgcagcagc
     3301 gcagcgtctc gctcagcgag gccatcacga tggcgcagcg cgtgcagccc gctaccgtgc
     3361 aggcgcaggg ggccattcgg agcgccgatg cgcagaaact cgccgcgtat ggcgaattca
     3421 tgccctcggt gaactacacg gcctccgcct cgacggcgca caacgacctg ccatcatata
     3481 ccgaccagaa cgggcaggtg cattcgaagt cgtcgacatc gaacacgttc tccaacggca
     3541 tctccgccag catcgacctt ttcaccggcc tccggcgcgg cgcagacctt tcggcggcaa
     3601 ccgcgtccca acgcgccgcc gatgccggcc tcgtcaacgc gcgctaccag gtggcgctca
     3661 ccaccaccca gtcgttcatc accgcgctca c
//
LOCUS       DASUAP010000188         6902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_223728, whole genome shotgun sequence.
ACCESSION   DASUAP010000188 DASUAP010000000
VERSION     DASUAP010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223728"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFU45_04680"
     CDS             complement(<1..483)
                     /locus_tag="VFU45_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU5358391.1"
                     /translation="MKALRVALITVACVAALVAAAAIGGRWYLARSVAQESGTARAPG
                     LGAPVDITFDAKGIPQVWAATSNDAIYTLGWLHASERLFQMELIRRVAEGRLAELFGP
                     GALPLDTLARRMGFARQGRREVGDLDPATREMLEHYAAGINAWIAAARVLPPEFVLLR
                     T"
     gene            complement(491..1258)
                     /locus_tag="VFU45_04685"
     CDS             complement(491..1258)
                     /locus_tag="VFU45_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5358392.1"
                     /translation="MTPPRPWLLGESTWAEVRATRYAVAVLPWGATEAHNRHLPYATD
                     TIETSAVAAESARIAWERGARVVVLPAVPFGVHTGQLDIPLCLNANPTTQFALLSDLA
                     DSVGRAGIEKLVLINGHGANDFRQMIRELQGRLPLFICTINWYAAVDPQAHFTEPGDH
                     AGESETSVTMHLAPGLVRPLAEAGPGAARRFRPAGLREGWAWAPRQWSRVTNDTGVGD
                     PRAATAEKGARYFAAVTDRIGGFLHELAGTRLDDLYE"
     gene            complement(1264..1644)
                     /locus_tag="VFU45_04690"
     CDS             complement(1264..1644)
                     /locus_tag="VFU45_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5358393.1"
                     /translation="MSTDDRLRPHPHDRLNEAIHRIDLAQAIRDLRNEPLTAVDGHRQ
                     ITLVRDGPVSLILFAFEPGATLKEHRAPGVISIHVLSGRLQVAAGGTTHDLPAGTVLA
                     LGPGAPHGVHAVDRSDMLLTVART"
     gene            complement(1747..3024)
                     /locus_tag="VFU45_04695"
     CDS             complement(1747..3024)
                     /locus_tag="VFU45_04695"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5358394.1"
                     /translation="MSAPPSADRGRFATLGRALRHRNYRLFFGGQGTSLVGTWITRIA
                     TSWLVYRLTGSALLLGLVGFAGQIPTLILAPFAGVLVDRWDRHRILVITQVLSMLQSL
                     ALAVLAIPHIITVTDILALQLVQGVINAFDTPARQSFLVEMIEDRGDLPNAIALNSTL
                     VNASRIIGPSLGGVLIALVGEGWCFMVDAVSYVAVVWALQAMRFVRVERPATTTRLSE
                     EFREGLAYVSGFPPARAVLLLIALVATMGMPYTVLMPAIAKTVLHGGPHTLGFLMAAS
                     GVGALCGALYLASRRSIVGLWRVVAMAGAAFGVGLVAFSLSRALALSLLILPVVGAGM
                     MVALASSNTILQTIVEERLRGRVMAFYTMAFLGTAPIGSLIAGVVADRIGSPATVLLG
                     GIACVLGAGIFALTLPRLRPHIRAVYAERGVVR"
     gene            complement(3002..3577)
                     /locus_tag="VFU45_04700"
     CDS             complement(3002..3577)
                     /locus_tag="VFU45_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEU5358395.1"
                     /translation="MPTLTLDPKTTALVLIDLQQGITAIATAPHSVADVIARAAALAR
                     RFRERGATVVLVRVEPGRHGELMPSPQADRPRPKMEYPADWCELVPELGRAETDIVVT
                     KHQPNAFYATDLEVQLARRGIRTIVLGGISTNIGVEATARAAHERGYEQVFVEDAMAA
                     READLHAFTTGRFLPTIGRVRPASDVSAALG"
     gene            complement(3755..4144)
                     /locus_tag="VFU45_04705"
     CDS             complement(3755..4144)
                     /locus_tag="VFU45_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5358396.1"
                     /translation="MTSPKAWYGAHADTLYAVMRIVVAFLFLSHGLQKHLPVFGGPMA
                     RGGPWLFQLAGVIEITCGTAILIGFLTSWAAFIAAGEMACAYFIVHIHMGAWPILNHG
                     EIVVAFCFVFLYISARGAGPWSVDGNK"
     gene            complement(4238..4657)
                     /locus_tag="VFU45_04710"
     CDS             complement(4238..4657)
                     /locus_tag="VFU45_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358397.1"
                     /translation="MPSKVSFNGVDYDGIDAMPAEVRQEYEKVLGLLSPEDRARLEER
                     EAGSSSLKLSLNVHTRFKVGGKEYDNVDQMPPAVRAAYDRVLSGRGTPPGPSITPGAP
                     TIPPRDDGDQPDRRRQLLFAFAFAILLAAAVLVLRAR"
     gene            4968..5174
                     /locus_tag="VFU45_04715"
     CDS             4968..5174
                     /locus_tag="VFU45_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU5358398.1"
                     /translation="MRVKGTVKWFNDAKGFGFITPEDGSKDCFVHHSAIQAQGFKSLA
                     EGEKVEFDIVQGQKGPAAENVVKL"
     gene            complement(5242..6786)
                     /locus_tag="VFU45_04720"
     CDS             complement(5242..6786)
                     /locus_tag="VFU45_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358399.1"
                     /translation="MRPVRTLSALLAAALIAAPAVAQRPERGARPGAAAADTSKPANS
                     TTPQEDSSATEHSITIGGKPVPYRALAATMLLKNDSGQAIGSMYFTAYTRTDTRDVSH
                     RPITFIYNGGPGSSTVWLQMGAFGPRKVVTSDASPTPPAPYTLVDNANSLLDVSDLVF
                     IDPIGTGFSKLVGKGTGKDFWGVDEDAASIEQFISNYVSRYGRWNSPKYLLGESYGTT
                     RSAVLVNRLQERDGMDFNGVVLISSVLDFETLNFATGHDISYALYLPSYAATAVYHKV
                     IPAPADLPAFLQQVREYAEGPYAAALDQGLSISDAEKAAVAKQLSAYTGLSEDYLMKA
                     NLRVSLGQFMEELQRSKGVIVGRLDSRFTGPTFDLLSEEAEYDPQSNAITGAYTAVWN
                     SYVRDELKYGKDKTYATMGNVRGWNWNRGRGGWTTATYVGGDLARALVSNPNLKVQVE
                     NGYYDLATPFMATEYTMDHLPMQPAARSRIELKYYDAGHMMYVYEPALTKLKANVAAF
                     IQSTHQ"
BASE COUNT         1062 a         2242 c         2596 g         1002 t
ORIGIN      
        1 cgtccgcagg aggacgaact cgggcgggag gacccgggcg gccgcgatcc aggcgttgat
       61 gccggcggcg tagtgctcca gcatttcccg ggtggcgggg tcgaggtcgc cgacctcgcg
      121 gcggccttgg cgggcaaacc ccatccggcg ggccagcgtg tcgagcggga gggctccggg
      181 tccgaagagc tccgccagcc ttccttccgc gacgcggcgg atgagctcca tctggaacag
      241 gcgctcggag gcgtggagcc agccgagggt gtagatcgcg tcgttgctgg tggcggccca
      301 gacctgcggg atccccttgg catcgaacgt gatgtcgacc ggcgcgccga ggccgggggc
      361 ccgggcggtg cccgattcct gcgccaccga ccgggcaagg taccagcggc cgccgatcgc
      421 ggcggccgcg accagcgcgg cgacgcacgc caccgtgatc agggcgacgc gcagggcctt
      481 cacggctcgg tcattcgtag aggtcgtcga gacgggtgcc ggcgagctcg tggaggaaac
      541 cgccgatccg gtccgtcacc gcggcgaagt agcgggcgcc cttctcggcg gtggcggcgc
      601 gcgggtcgcc gacgccggtg tcgttcgtga cccggctcca ctggcgcggc gcccaggccc
      661 acccctcccg gagtccggcg ggcctgaagc gccgggcggc gccgggcccg gcctcggcga
      721 gagggcggac gaggcccggc gccaggtgca tcgtgacgct cgtctccgat tcgccggcgt
      781 ggtcgcccgg ttcggtgaag tgcgcctgcg ggtcgaccgc ggcataccag ttgatggtgc
      841 agatgaagag gggaaggcgt ccctgcagct cccggatcat ctgccggaaa tcgttcgcgc
      901 cgtggccatt gatgaggacc agcttctcga tcccggcgcg gccgaccgaa tcggccagat
      961 cggaaaggag cgcgaactgc gtggtcgggt tcgcgttgag gcagagcggg atgtcgagct
     1021 ggccggtgtg gacaccgaac gggaccgccg ggaggacgac gaccctggcg ccccgctccc
     1081 acgcgatccg tgccgactcg gcggcgacgg ccgaggtctc gatcgtgtcg gtggcatagg
     1141 gcaggtggcg gttgtgcgcc tccgtggcgc cccaggggag caccgccacc gcgtatcgcg
     1201 tggcccggac ctccgcccac gtcgattccc cgaggagcca cgggcggggc ggcgtcacgg
     1261 cgctcaggtc cgcgcgacgg tgaggagcat gtcggaccgg tccaccgcgt gcaccccgtg
     1321 cggggcaccg gggccgagcg ccaggaccgt cccggccggc aggtcgtggg tcgtgccgcc
     1381 cgccgccacc tgcaggcgac cggacaggac gtggatgctg atgacgccgg gcgcccgatg
     1441 ctccttcagg gtggcgccgg gttcgaaggc aaagaggatg aggctcaccg ggccgtcacg
     1501 gaccagcgtg atctgccggt ggccatccac ggcggtgagg ggctcattgc ggaggtcccg
     1561 gatggcctgg gccaggtcga tccggtggat ggcctcgttc aggcggtcgt gcgggtgcgg
     1621 gcgcagccgg tcatcggtgc tcatcgcgct actcctggcg gacgtgttcc agcggaaggt
     1681 ctgcaggcga atccgaggat agcggcgggg ccgggagggc gccagcgatc ccggaccgcg
     1741 acagtgtcac cgcaccaccc cccgttccgc gtataccgcg cggatgtggg ggcggagccg
     1801 cggcagcgtg agcgcaaaga tccccgcgcc caggacgcag gcgatcccgc cgaggaggac
     1861 cgttgcgggc gagccgatcc ggtcggccac gacgccggcg atgaggctcc cgatcggcgc
     1921 ggtgccgagg aacgccatcg tgtagaaggc catcacgcgg ccccgcaggc gctcctcgac
     1981 gatcgtctgg aggatcgtgt tgctggaggc gagcgccacc atcatcccgg cgccgaccac
     2041 cggcaggatg aggagcgaga gggcgagtgc ccgcgagagg gagaacgcca ccagcccgac
     2101 cccgaacgct gcccccgcca tcgcgacgac ccgccacaac ccgacgatgc tccggcgcga
     2161 cgcgaggtag agcgcaccgc agagggcacc cacgccggag gccgccatca ggaacccgag
     2221 cgtgtggggg ccgccgtgga gcaccgtctt ggcgatggcg ggcatcagca cggtgtaggg
     2281 catccccatc gtggcgacca gggcgatcag gagcagcacc gcgcgggccg gcgggaagcc
     2341 ggagacgtac gccagcccct cccggaactc ctcgctcagc cgcgtcgtgg tcgccggacg
     2401 ctcgacgcgc acgaaacgca tcgcctggag cgcccagacc accgccacgt agctcaccgc
     2461 gtccaccatg aagcaccatc cctcgcccac cagcgcgatg agcaccccgc ccagcgaggg
     2521 gccgatgatc cggctggcat tcaccagcgt cgaattgagg gcgatggcgt tggggaggtc
     2581 gccgcggtcc tcgatcatct ccaccaggaa cgactggcgc gccggggtgt cgaaggcgtt
     2641 gatgaccccc tggaccagct gcagcgccag gatgtcggtg acggtgatga tgtgggggat
     2701 ggcgagcacc gcgagcgcca gcgattggag catcgacagc acctgggtga tcaccaggat
     2761 gcggtgcctg tcccaccggt ccaccagcac gcccgcgaag ggcgccagga tgagggtggg
     2821 gatctgtccg gcgaatccca ccaggccgag gaggagggcg gaaccggtga gccggtacac
     2881 cagccagctc gtggcgatgc gggtgatcca cgtgccgacg agcgacgtgc cctgcccgcc
     2941 gaagaagagg cgatagttgc ggtgccggag ggcccggccg agggtcgcga agcggccgcg
     3001 gtcagccgag ggcggcgctg acatcgctgg ccgggcgcac ccggccgatc gtcggaagga
     3061 agcgtcccgt ggtgaaggcg tgcaggtcgg cctcgcgggc ggccatcgcg tcctcgacga
     3121 agacctgctc gtagccgcgt tcgtgggcgg cgcgcgcggt ggcctcgacg ccgatgttgg
     3181 tgctgatgcc gccgagcacg atggtacgga tcccgcggcg ggccagctgg acctcgaggt
     3241 cggtggcgta gaaggcgttg ggctggtgtt tggtgaccac gatgtcggtc tcggcacgcc
     3301 cgagctccgg gacaagctcg caccagtcgg cggggtactc catcttcggc cggggtcggt
     3361 cggcctgggg ggagggcatc agctccccgt ggcggccggg ctcgacccgg accaggacga
     3421 ccgtggcacc gcgctcccgg aagcggcggg ccagcgcggc ggcgcgggcg atgacgtcgg
     3481 cgaccgaatg gggggctgtc gcgatggccg tgatcccctg ctgcaggtcg atcagcacca
     3541 gggcagtggt cttcgggtcg agggtcaggg tcggcatcgg ggtcccctca ggcggagcgt
     3601 gggaccacgg agggcacgga ggagcacgga gagtcacgga gaactgcatt tgaagtggat
     3661 tcacgaacgg ggctccgctc gcgagaccac agaagagcct ttcctccgtg cctctccgtg
     3721 cgctccgagc cctccgtggt cccagttgag tcagtcactt gttcccgtcc acactccacg
     3781 gacccgcgcc gcgggcggag atgtagagga acacgaagca gaacgcgacc acgatctcgc
     3841 catggttcag gatgggccag gcgcccatat ggatgtggac gatgaaatag gcgcaggcca
     3901 tctccccggc cgcgatgaac gccgcccacg aggtcaggaa cccgatcagg atcgcggtgc
     3961 cgcaggtgat ctcgatgacg ccggccagct ggaagagcca gggtccgccc cgcgccatcg
     4021 ggccgccgaa gacgggcaag tgcttctgga ggccgtggct caggaagagg aacgcgacga
     4081 cgatgcgcat cacggcatag agcgtgtccg cgtgggcgcc gtaccaggcc ttgggggatg
     4141 tcatgcgggc tcggagagtg gcgggtggtg ggtggtgcgc catcgccgaa gagtaaggcc
     4201 tggccgacgg aacgggaggc ggcggaccgc ggcgcgatca gcgggcgcgc agcacgagca
     4261 ccgccgcggc gagcaggatg gcgaacgcga acgcgaagag cagctggcgg cggcggtcgg
     4321 gctggtcgcc gtcgtcacgc ggcgggatcg tcggggcacc gggcgtgatc gacggcccgg
     4381 gcggggtccc cctgccgctg agcacccggt cgtaggccgc gcgcactgcg gggggcatct
     4441 ggtcgacgtt gtcatactcc ttcccgccga ccttgaaccg ggtgtggacg ttgaggctga
     4501 gcttgagcga ggagctcccc gcctcccgct cctcgagccg ggcccggtcc tcggggctca
     4561 ggagccccag caccttctcg tactcctgcc ggacctccgc cggcatcgcg tcgatcccgt
     4621 cgtagtccac gccgttgaag ctgaccttgc tgggcacggt atccctcctg ccgggtggcg
     4681 ggtggtcggg ggggggggtc gggcggaaag accgccattc cggcctcgcg cggcgatccg
     4741 gcctcctgga acttggggtc cggtccgggg cccggcaagc gccctccggg ggggcttgca
     4801 gcgccaaagt ccggaccagc ttatcttaaa ggcgttgtgt ccggcaggac ggcggtccac
     4861 gccgccccgg gcacggcgcc cctcgtgagg ctcgttcacg aacaaccatt gtagtcgggt
     4921 gagacggcgc gtccggactc cgattacgaa cagcaaggag tacggcaatg cgcgtcaagg
     4981 gcaccgtgaa gtggttcaac gatgcgaagg gcttcgggtt catcaccccc gaagacggct
     5041 cgaaggactg cttcgtccat cactcggcca tccaggcgca gggcttcaag tccctcgccg
     5101 agggtgagaa ggtcgaattc gacatcgtgc agggccagaa gggccccgct gccgagaacg
     5161 tcgtcaagct ctaagcgcct gacggctgga acgatgaagg ccggtccgtc tcacgacgga
     5221 ccggccttta tcgttcccga cttactggtg ggtcgactgg atgaacgcgg ccacgttggc
     5281 cttgagcttg gtcagcgccg gctcatagac gtacatcatg tgcccggcgt cgtagtactt
     5341 gagctcgatc cggctgcggg ccgcgggctg catcggcagg tggtccatcg tgtactcggt
     5401 ggccatgaac ggcgtcgcga ggtcgtagta gccgttctcc acctgcacct tgaggttcgg
     5461 gttggacacc agcgcccgcg ccaggtcgcc gccgacgtag gtcgcggtgg tccagccgcc
     5521 gcggccgcgg ttccagttcc agccgcggac gttgcccatc gtcgcgtagg tcttgtcctt
     5581 cccgtacttc agctcatcac gcacgtagct gttccacacc gccgtgtacg cgccggtgat
     5641 ggcgttcgac tgcggatcgt actcggcctc ctcgctcagc aggtcgaagg tcggaccggt
     5701 gaaccgcgag tcgagccggc ccacgatgac gcccttcgac cgctgcagct cctccatgaa
     5761 ctggcccagc gagacgcgga ggttggcctt catcaggtag tcctcggaga gcccggtgta
     5821 cgccgagagc tgcttggcca ccgcggcctt ctcggcgtcg ctgatggaga gcccctggtc
     5881 gagcgcggct gcgtacggcc cctcggcata ctcccgcacc tgctggagga aggccggcag
     5941 gtcggccggc gccgggatga ccttgtggta caccgcggtc gccgcgtagc tcgggaggta
     6001 gagcgcgtag gagatgtcgt gcccggtggc gaagttgaga gtctcgaagt cgagcaccga
     6061 ggagatcagg acgacgccgt tgaagtccat cccgtcgcgt tcctgcagcc ggttgacgag
     6121 caccgccgag cgggtggtgc cgtagctctc gccgaggagg tacttgggcg aattccagcg
     6181 gccgtagcgg gagacgtagt tgctgatgaa ctgctcgatc gaggcggcgt cctcatccac
     6241 gccccagaag tccttgccgg tccccttgcc gacgagcttg ctgaagccgg tgccgatcgg
     6301 gtcgatgaac accaggtccg acacgtcgag gagcgagttg gcattatcca ccagcgtgta
     6361 cggggcaggt ggggtggggg aggcgtcgga ggtgaccacc ttccgcgggc cgaacgcgcc
     6421 catctggagc cagacggtcg aggaaccggg gccgccgttg tagatgaacg tgatcggccg
     6481 gtggctcaca tcgcgggtgt cggtccgggt gtaggcggtg aagtacatcg aaccgatggc
     6541 ctggcccgag tcgttcttga ggagcatcgt ggccgcgagg gcgcggtagg gcaccggctt
     6601 cccgccgatg gtgatgctgt gctccgtggc cgacgaatct tcctgcggcg tcgtggagtt
     6661 ggcgggcttg ctcgtgtcgg cggcggcggc cccgggccgg gccccgcgtt cggggcgctg
     6721 ggccacggcg ggtgcggcga tgagggcggc cgcgaggagc gcggacaacg tacggacagg
     6781 acgcatcagt cctccagaag gtgttgggga tgggcgcacg gcgcccagcc ggagtgtcgg
     6841 agcagcggtg gtgatggggt ggaaggtacc cccaacgtac gctccaggcg gccatttggt
     6901 tg
//
LOCUS       DASUAP010000189         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1109043, whole genome shotgun sequence.
ACCESSION   DASUAP010000189 DASUAP010000000
VERSION     DASUAP010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109043"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /gene="murC"
                     /locus_tag="VFU45_04725"
     CDS             <1..1270
                     /gene="murC"
                     /locus_tag="VFU45_04725"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU5358400.1"
                     /translation="TVTGCDNDPSGAADVAALGATVLAGHDPAHVAGARAVVVTAAIP
                     NEHPELVRARELGIPVLRRKQALAELVRGGRVVGVSGTHGKTTTTVMATEALTAAGLA
                     PTGLAGGRVAAWGGNAKLAGDALFVVEADEYDQAFLALHPAVAVVNNVEADHLECYGT
                     LEALEQAFIEFAGRAGRALVGTDDPGAARVAAQLTVPVWRFGQGDADLRIVTVDAGPE
                     GSTARVRMPDGSDVTLRLRVPGLHNVKNATAALGVVAALGGQVAPALEALAGFTGAGR
                     RFERLGAARGVTFVDDYAHHPSELAATLQAARQAFPGRRLIAVFQPHLYSRTDRHGEA
                     MGQALAAADLAIVTEIYAAREAPIPGVSGRRVAQAVAASGGRAVFEPEFDRVGGRVTE
                     LATKGDVVLTLGAGDITRVGRELVAAWRAS"
     gene            1255..1941
                     /locus_tag="VFU45_04730"
     CDS             1255..1941
                     /locus_tag="VFU45_04730"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEU5358401.1"
                     /translation="MARFLKPGWLALYGACVALGVWFGAPRLFRRMEFFRVRRVEVLG
                     LHSLAVADVLRALAIPARENVFDDLRPIAARAAAIAGVAHAEVTRRIPGTIRVTLQEV
                     VPVALVPGHGALVPMDAKGQVLPFDPAVAAPDLPVTPRADSLVGELLGRVQEADPGLF
                     EQVVTTERDGRDVALDLGAHRYWFRPDATVETIRAVTAVAQDLARRGRAFRELDGRYA
                     GQVVVRWGTA"
     gene            1938..>2753
                     /gene="ftsA"
                     /locus_tag="VFU45_04735"
     CDS             1938..>2753
                     /gene="ftsA"
                     /locus_tag="VFU45_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU5358402.1"
                     /translation="MSTAPQRLVAALDLGSTKVTAIVAEMTGDARNPGAKILGVGVER
                     SGGVRRGVVRDIEETTRAITKAMRDAQRMAGVEVGSVYCGVAGEHVAGRTTKGLVSVT
                     GAEIRTGDVARVNDVASNVSFGRDHELLHAIPQEYLIDEQPGINDPIGMTGSRLEAEV
                     YLVTVMASVPQNLRKCVERAGFRVEEFVLEPLAASLAVLTPDELELGCALVELGGGST
                     NVAIFQNGKIRHTASLLCAGGHVTSDIVHGLQVTQQDAERLKERFGAAYEPLVP"
BASE COUNT          358 a          910 c         1118 g          367 t
ORIGIN      
        1 gacggtgacc gggtgcgaca acgatccgag cggcgccgcc gacgtggcgg ccctgggcgc
       61 caccgtcctg gcgggacacg atcccgccca cgtggccggc gcccgcgcgg tggtggtcac
      121 cgcggcgatc ccgaacgaac atcccgaact ggtccgcgcc cgggaactcg gcatccccgt
      181 gctgcgacgc aagcaggcgc tggcggagct ggtgcgcggc ggccgggtgg tgggcgtgag
      241 cggcacccac ggcaagacca cgacgaccgt gatggcgacc gaggcgctga ccgcagccgg
      301 actggcgccg accgggctcg ccggcggaag ggtggccgcc tggggtggca acgccaagct
      361 ggcgggcgat gcgctcttcg tggtcgaggc cgatgaatat gaccaggcct tcctggcgct
      421 ccaccccgcg gtggcggtgg tgaacaacgt cgaggccgac cacctcgagt gctacgggac
      481 cctggaggcg ctggagcagg ccttcatcga gttcgcgggc cgggccggca gggcgctggt
      541 gggcaccgac gacccgggcg cggcgcgggt ggccgcccag ctcacggtgc cggtgtggcg
      601 gttcggccag ggcgatgccg accttcgcat cgtgacggtc gatgcgggtc cggaagggtc
      661 caccgcccgg gtgcggatgc ccgacggcag cgacgtcacg ctccgcctca gggtgcccgg
      721 tctccacaac gtgaagaacg ccacggctgc gctgggcgtg gtcgcggcgc tcggcgggca
      781 ggtggccccg gcgctcgagg cgctggcggg attcaccggg gcggggcggc ggttcgagcg
      841 gctgggtgcg gcacggggcg tcaccttcgt ggacgattac gcgcatcatc cgtccgaact
      901 cgcggcgacg ttgcaggcgg cgcggcaggc gttcccgggg cggcggctga tcgcagtgtt
      961 ccagccgcac ctctattccc gcaccgaccg gcacggtgag gcgatggggc aggcgctcgc
     1021 ggcggcggac ctcgcgatcg tgaccgagat ctacgcggcc cgggaggcgc cgataccggg
     1081 ggtgagcggc cggcgggtgg cccaggcggt ggcggcgagc ggtggccggg cggtgttcga
     1141 gcccgagttc gaccgggtag gagggcgcgt gacggagctg gcgacgaagg gcgacgtcgt
     1201 cctgacgctc ggggccggtg acatcacccg cgtcggacgg gagctggtgg cggcatggcg
     1261 cgcttcctga agcccggctg gctcgctctg tacggggcgt gcgtggcgct gggggtgtgg
     1321 ttcggcgccc cgcggctctt ccggcggatg gagttcttcc gggtgcgtcg ggtggaggtg
     1381 ttggggcttc attcattggc ggtcgccgac gtgctccgcg ccctggcgat cccggcccgc
     1441 gagaacgtct tcgatgacct ccggccgatc gcggcacggg ccgcggccat cgccggggtg
     1501 gcccacgccg aggtgacgcg ccggatcccc ggcaccatcc gggtgacgct gcaggaggtg
     1561 gtgccggtgg cgctcgtccc gggacacggg gcgctcgtgc cgatggacgc gaaagggcag
     1621 gtgctcccct tcgacccggc ggtggcggcg cccgaccttc cggtgacgcc gcgggccgat
     1681 tcgctggtgg gcgagctcct cgggcgggtg caggaggccg atcctgggct cttcgagcag
     1741 gtggtcacca ccgaacggga cggccgggac gtggcgctcg acctgggggc gcaccggtac
     1801 tggttccgtc ccgacgccac ggtggagacg atccgggcgg tgacggcggt ggcgcaggac
     1861 ctcgcgcggc gcggccgcgc gttccgggaa ctcgatggcc ggtacgccgg ccaggtcgtg
     1921 gtcagatggg ggaccgcttg agcactgcac cgcagcggct cgtcgccgcg ctcgacctgg
     1981 gatccaccaa ggtgacggcc atcgtggccg agatgaccgg tgatgcgcgg aacccggggg
     2041 ccaagatcct cggggtgggc gtcgagcgga gcggcggggt ccgccggggc gtggtccggg
     2101 acatcgagga gaccacgcgg gccatcacca aggcgatgcg cgatgcgcag cggatggcgg
     2161 gcgtcgaggt cgggtcggtg tactgcggcg tcgccggcga gcacgtggcg gggcgcacca
     2221 ccaaggggct ggtgtcggtc accggcgcgg agatccgcac cggcgacgtc gcgcgggtca
     2281 acgacgtggc gagcaacgtc tccttcggcc gggaccacga gctgctgcac gccatcccgc
     2341 aggaatacct gatcgacgag cagccgggga tcaacgaccc gatcggcatg accgggtccc
     2401 gcctcgaggc cgaggtgtac ctggtcacgg tcatggcgag cgtgccgcag aacctgcgga
     2461 agtgcgtcga gcgggccggg ttccgggtcg aggagttcgt gctggagccg ctggcggcgt
     2521 cgctcgcggt gctcacgccc gacgagctcg agctcggctg cgcgctggtg gagctggggg
     2581 gcgggagcac caacgtggcc atcttccaga acgggaagat ccggcacacc gcctcgctcc
     2641 tctgcgcggg gggccacgtc accagcgaca tcgtgcacgg cctccaggtg acccagcagg
     2701 acgccgagcg gctcaaggag cgcttcggcg cggcgtacga gccgctcgtc ccc
//
LOCUS       DASUAP010000190         7059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_700269, whole genome shotgun sequence.
ACCESSION   DASUAP010000190 DASUAP010000000
VERSION     DASUAP010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700269"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFU45_04740"
     CDS             <1..542
                     /locus_tag="VFU45_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358403.1"
                     /translation="GGPGGLREVSVRRGEGSGLWGSFALGWGREGFRFDSMMTYQSDF
                     SAPTFTFKLGGTPSRYFRLGGELSYWYSSEAQSDQTLANYLFIAQWYPGAHPFYVKAG
                     LGLVSNHQSGSDPVNGTYVYRDNGYGGVIGIGADIPVGRRLALSPELNVYGQRYDNAG
                     TSNDYRERIVTFGLGITFR"
     gene            650..731
                     /locus_tag="VFU45_04745"
     tRNA            650..731
                     /locus_tag="VFU45_04745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:684..686,aa:Leu,seq:gag)
     gene            771..842
                     /locus_tag="VFU45_04750"
     tRNA            771..842
                     /locus_tag="VFU45_04750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:803..805,aa:Thr,seq:cgt)
     gene            complement(902..2644)
                     /locus_tag="VFU45_04755"
     CDS             complement(902..2644)
                     /locus_tag="VFU45_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU5358404.1"
                     /translation="MKGAARSLLEHPMLRRYTSLVREDLAATYSRDLHKWLLVAPILG
                     VVTGLLITLVAQVILRWLWPAILAYDLQNPAAMVPIVTAGFLVAGLIMQHLTPDPDLH
                     STEEIIRSYHEHQGEVQLRPFIPKLLAAIATVGAGGSAALEGPSIYGGGAVGSWLWTK
                     LRKVGLGPRDRRIMLISGAAAGMSAVFRAPLTGIIFALEMPYQDDLAHEALMPALIAS
                     VVSYATMAAFLGSAPLFDFAAAAGYTRRDLLWAAGLGILCGLVAMAFAITFRRLRTFS
                     VRLKVPHWLKMTAGGLLTGLCGLAFLSIFPGKLVPIGPNYEAVSQILQAGHGTAELLA
                     FGALKLVATLFSLGVGGVSAMFVPLFLAGSSFGTAFGQSVVHSTNPELYAAVGMAAFI
                     AAAYKAPLTAVVFVAEATGGHGFIIPALIGAAVAYGVSGAASVSGDQRLHEAVRVAAL
                     GGVAVADAMQARVTTTGVDTTVREFVGGIADGATPHTVYPVVEDGRVVGTLAASTISR
                     VSPDRWDEVRVHELMDREFTSVAPDASVQDALRLLRGTGAPHLVLVVAAGGRLAGIVT
                     KTDVLAVLDTRGRG"
     gene            complement(2641..3042)
                     /locus_tag="VFU45_04760"
     CDS             complement(2641..3042)
                     /locus_tag="VFU45_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5358405.1"
                     /translation="MRPTTAIRRLAQFRYALRRFLRFSEQAARAAGVTPLQHQLLLGV
                     AGFTGRGWATISELAECLQERHNGVGALVNRAVRAGLVRKGKGGGDRRVVRVSLTPRG
                     RRIIEGLTRLHQRELAGFQRDLPPVLARAVA"
     gene            3161..4417
                     /locus_tag="VFU45_04765"
     CDS             3161..4417
                     /locus_tag="VFU45_04765"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5358406.1"
                     /translation="MPALRHDPYAALRHADFRWFIIALTTMTVAVTMRGIVVGWQVYS
                     VTHDPLSLGLIGLAEALPFIASALFAGHVADRTDRRRITMLAMVVLLACAGGLLALSG
                     RGPLGRTDIRWFYLVIGVGGVARSFLMAARTALAAELVPREHYGNASAWRTSSWQFAA
                     VAGPACGGVIYGLAGPVAAYGTDLALMVVAVLAIAQVRPRGRPPEADVREPLAQSLSG
                     GIRFLRGQPVVLGAMTLDLFSVLFGGAVALLPVYAADILRVGPAGLGILRAAPAAGAV
                     VTSLWLTHRPPFRRAGLAMLGNVATFGLSVIAFGISRNFALSALFLAIYGGVDVVSVV
                     IRATLVQEFTPHAVMGRVSAVNSIFIGSSNEIGAFESGLAAKLMGTVPSVVFGGTVTL
                     AVVALTAWRVPSLRRLREIKAPAASS"
     gene            4513..4944
                     /locus_tag="VFU45_04770"
     CDS             4513..4944
                     /locus_tag="VFU45_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5358407.1"
                     /translation="MRSKRSTLAPIVLSALCVAAAPLSAQQKGTARAPVPKDSMAGAG
                     PAAAELLSAKEISAGRDVFHGEGNCFVCHGTALEGGPIAPTLRAHQWRNGDGSFDMIF
                     HVVTTGVPNTAMVSHPGGISDAEARQVAAYVWAVSHGKAKP"
     gene            complement(5019..6395)
                     /locus_tag="VFU45_04775"
     CDS             complement(5019..6395)
                     /locus_tag="VFU45_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358408.1"
                     /translation="MSLLAVPTTALHAAGSGDAVRTYARRYAEGRATASIHFAAIPSF
                     SRQTGLACNACHTAFPRLTAFGRLFKLNGYTLTGLQVVTAGAEGQRQSLKLDLIPPIS
                     AMAVSSYSQTAKDVPGTQNGVVQLPQQLSLFFGEAITPRLGTFMQVTYDPAAGTIGMD
                     NLDVRFADHTRFANKQLLYGLTLNNNPTVQDVWNTAPAWRFPYMSSAVAPTPAAATLI
                     DGGLGQSVAGLGAYGLWDNLVYAEASFYRSAAQGGPNPPDSTSSMTIKGGAPYWRLAV
                     QHQWSNDYLELGTYGMSTALFPVGVSGPTDRYTDIGFDAQYERPIGSTNLTLHGAYIT
                     ESQRLDGSVAAGAASNLSDKLHTFRVDGSLLTAKRVGLTLSYFTTTGDADALRYPAGA
                     IFGSGTASPNSDGMMAELEALPWLNTRFALQYVAYTKFNGGTTNYDAAGRNASDNNTL
                     YLYTWLVF"
     gene            6721..6794
                     /locus_tag="VFU45_04780"
     tRNA            6721..6794
                     /locus_tag="VFU45_04780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6755..6757,aa:Pro,seq:ggg)
BASE COUNT         1014 a         2414 c         2516 g         1115 t
ORIGIN      
        1 tcggcggtcc cggcgggttg cgggaagtga gcgtccgccg tggggaaggg agcgggttgt
       61 gggggtcgtt cgcgctcggc tggggcaggg aaggcttccg gttcgacagc atgatgacct
      121 accagagcga cttctccgcg ccgaccttca cgttcaagct cggcggcaca ccaagccgct
      181 acttcaggct cggcggggag ctttcctact ggtactcgag cgaggcgcag agcgaccaga
      241 cgctcgccaa ctatctgttc atcgcccagt ggtatccggg tgcgcatccg ttctacgtca
      301 aggccgggct cggcctggtg agtaaccatc agagcggctc cgatcccgtc aacggcacct
      361 atgtctatcg cgacaacggg tacggcggcg tgatcggcat cggggccgat atccccgtcg
      421 ggcggcgcct ggccctctcg cctgaactca acgtgtacgg gcagcgttac gacaacgccg
      481 gcaccagcaa cgactatcgc gaacggatcg tcaccttcgg gctggggatc acgttcaggt
      541 aattcccggg ggacgcccgt cctccggtgc cccggtcggc cgccctggcc ggccggggca
      601 ttcttttggc ttgattcttc acgtggtggc ggctagaatt gccccgcgcg ccctggtggt
      661 gaaactggta gacacgctat cttgaggggg tagtgccgaa aggcgtcgcg gttcgaatcc
      721 gcgccagggc atagcgaggg gcgaccatcg tgtcgcccct ttgcagtacc gccactgtag
      781 ctcaggggta gagcactcga ttcgtaatcg agcggtcgtc ggttcaaatc cgaccagtgg
      841 cttgccgtcc ccccgcccgt cccctcgccg ccgcacccgg ttccgccagg ccgaccgcgc
      901 cttacccgcg tccccgcgta tcgagcactg ccagcacatc cgtcttggtg acgatcccgg
      961 caagccgacc gccggcggcc accaccagca cgagatgggg cgcccccgtg cctcgcagca
     1021 ggcggagcgc atcctggacc gatgcatccg gcgcgacgct cgtgaactcc cggtccatca
     1081 gctcgtggac ccggacctcg tcccatcggt ccggcgacac gcgggagatc gtgctcgcgg
     1141 cgagcgtgcc caccacccgg ccgtcctcga ccaccggata gacggtgtgc ggggtcgcgc
     1201 cgtccgcgat cccccccacg aattcccgca ccgtggtgtc gacgcccgtc gtggtgacgc
     1261 gggcctgcat tgcgtccgct accgccacgc ctccgagcgc ggccacccgc acggcctcgt
     1321 gcagccgctg gtcgccggac accgatgccg cgcccgacac gccataggcc accgccgccc
     1381 cgatgagcgc cgggatgatg aatccgtgtc cgccggtcgc ctcggccacg aagaccaccg
     1441 ccgtgagcgg cgccttgtac gcggcggcga tgaacgccgc cattcccacg gcggcgtaga
     1501 gctccgggtt cgtgctgtgc accaccgact ggccgaacgc cgtgccgaac gaactcccgg
     1561 ccaggaagag cgggacgaac atggcactca cgccgcccac gccgagcgag aagagcgtgg
     1621 ccaccagctt gagcgccccg aacgccagca gttccgcggt cccgtgcccg gcctgcagga
     1681 tctgtgacac ggcctcgtag ttggggccga tcggcacgag cttccccggg aagatcgaga
     1741 ggaatgccag gccgcagagg ccggtcagca acccgcctgc cgtcatcttg agccagtgcg
     1801 gcaccttgag ccggacggag aacgtccgca ggcgcctgaa cgtgatcgcg aaggccatgg
     1861 ccacgaggcc gcagaggatg cccagccccg ccgcccacag caggtcccgg cgggtgtacc
     1921 ctgcggcggc ggcaaagtcg aagagtggcg ccgacccgag gaacgccgcc atcgtggcgt
     1981 acgacaccac cgaggcgatg agcgcgggca tcaacgcctc gtgcgccagg tcgtcctggt
     2041 acggcatctc cagtgcgaag atgatgccgg taagcggcgc gcggaacacc gcggacatgc
     2101 ccgcagcggc accgctgatg agcatgatcc gtcggtcgcg gggcccgagc ccgaccttcc
     2161 gcagcttcgt ccacagccac gaccccaccg cgccaccacc gtagatgctc ggcccctcca
     2221 gcgcggcgct gccgccggcc ccgacggtgg cgatggcggc caggagcttg gggatgaacg
     2281 ggcgcagctg cacctcgccc tggtgctcgt ggtacgaccg gatgatctcc tcggtggaat
     2341 gcaggtcggg gtcgggtgtc aggtgctgca tgatgagccc ggccaccagg aaccccgcgg
     2401 tcacgatcgg gaccatggcg gccgggttct ggaggtcgta cgcgaggatc gccggccaga
     2461 gccaccggag gatgacctgc gcgaccaggg tgatgaggag accggtcacc acgcccagga
     2521 tcggcgccac cagcagccac ttgtggaggt cgcgagagta ggtcgccgcc aggtcctcgc
     2581 ggaccagcga cgtgtagcgc cgcagcattg ggtgctcgag cagcgagcgt gcggcgccct
     2641 tcatgcgacc gcccgcgcca gcaccggggg gaggtcgcgc tggaaccccg ccagctcccg
     2701 ctggtgcagg cgggtgaggc cctcgatgat ccggcggccc cgcggggtca gtgacacccg
     2761 gaccacgcgg cggtccccgc cacccttgcc tttgcggacg agcccggcgc ggacggcgcg
     2821 gttgaccagc gcccccaccc cgttgtgccg ttcctgcagg cactcggcca gctcggagat
     2881 cgtcgcccag ccgcgcccgg tgaagccggc gacgccaagc aggagctggt gctggagcgg
     2941 ggtgacgccg gcggcgcggg ccgcctgctc gctgaaccgg aggaagcggc ggagggcgta
     3001 gcggaactgc gcgaggcggc ggatggcggt ggtcggtcgc atgttgggtg ggtcaagaat
     3061 aatatcacgg cgtgatattt caacggccgt cccgccggtc gcccggcccc ggcccgagcc
     3121 ccgtcggccg caggccggtc tgccttatat tcccccgccg atgcccgccc tccgtcacga
     3181 cccctacgcc gccctccgcc acgccgactt ccgctggttc atcatcgccc tcacgacgat
     3241 gacggtggcg gtcacgatgc gcggcatcgt ggtcggctgg caggtctact cggtgaccca
     3301 cgatcccctt tcgctcgggc tcatcgggct cgcggaggcg ctgccgttca tcgcctcggc
     3361 gctcttcgcc gggcacgtgg ccgaccggac cgaccgccgg cggatcacga tgctcgcgat
     3421 ggtcgtgctc ctcgcctgcg ccggcggcct gctggcgctc tcggggcggg gcccgctcgg
     3481 tcgcaccgac atccggtggt tctacctcgt gatcggcgtg ggcggtgtgg cgcggagctt
     3541 cctgatggcg gcgcgcaccg ccctcgccgc cgaactcgtg ccccgcgagc actacggcaa
     3601 cgcatcggcg tggcgcacct cctcctggca gtttgcggcg gtggcgggcc cggcctgcgg
     3661 cggcgtcatc tacgggctcg caggcccggt ggccgcgtac gggaccgacc tggccctgat
     3721 ggtcgtggcc gtcctggcca tcgcacaggt ccggccgcgc ggccggccgc cggaggccga
     3781 tgtgcgggag ccgctcgcgc agagtctctc gggcgggatc cgcttcctcc gggggcagcc
     3841 cgtcgtcctc ggggcgatga cgctcgacct cttctcggtc ctcttcggcg gcgccgtggc
     3901 gctgctcccg gtctacgcgg ccgacatcct ccgggtcggg ccggcggggc tcggcatcct
     3961 gcgcgccgcc cccgcggccg gggcggtggt cacgtccctc tggctcaccc atcgcccccc
     4021 gttccggcgg gccggcttgg cgatgctcgg caacgtcgcg accttcggcc tctcggtcat
     4081 cgccttcggg atctcgcgga acttcgcgtt gtcggcactc ttccttgcca tctacggcgg
     4141 ggtcgacgtg gtgagcgtcg tgatccgcgc cacgctggtc caggaattca cgccgcacgc
     4201 ggtgatgggg cgggtctccg cggtcaattc gatcttcatc ggttcgtcca acgagatcgg
     4261 cgcgttcgag tcgggcctgg cagcgaagct gatgggcacg gtgccctcgg tggtcttcgg
     4321 gggcacggtc accctggcgg tggtggccct cacggcgtgg cgggtcccgt cgcttcggcg
     4381 cctgcgtgag atcaaggccc cggcggcctc gtcctgattc ctccccgatt ccttcatccg
     4441 gtgggtgggg ggatggtgcc ggacggggga atgccgcatt tttgtgcctc acactttcgc
     4501 cgggatggcc cgatgcgctc gaagcgttcc acccttgccc cgatcgtatt gtcggcgctc
     4561 tgcgtcgccg cggcgccgct cagcgcccag cagaagggca cggcccgtgc cccggtcccc
     4621 aaggactcga tggcgggggc tggtccggca gcggccgaac tcctgagcgc caaggagatc
     4681 tccgcggggc gggacgtgtt ccacggggaa gggaattgct tcgtctgcca cggcaccgcc
     4741 ctggagggtg gcccgatcgc gccgaccctg cgcgcgcacc agtggcgcaa cggcgatggc
     4801 agcttcgaca tgatcttcca cgtggtcacc accggcgtcc cgaacacggc gatggtgtcg
     4861 catccgggcg gcatcagcga cgccgaggcc cggcaggtgg cggcgtatgt ctgggccgtg
     4921 agccacggga aggcgaagcc ctgagcggct ccgccccgga acgacagcgg gcccggcatc
     4981 cagctgccgg gcccgctctc gtttccagcc tggtcaggtc agaagacgag ccaggtgtac
     5041 aggtacaggg tgttgttgtc tgacgcgttc cggcccgccg catcgtagtt ggtggtgcca
     5101 ccgttgaact tggtgtaggc cacgtactgc agcgcgaagc gggtgttgag ccagggcagg
     5161 gcctcgagtt cggccatcat cccgtcgctg ttcgggctcg cggtcccgct cccgaagatg
     5221 gcgcccgcgg ggtagcggag cgcatcggcg tcaccggtgg tcgtgaagta cgacagcgtc
     5281 aggccgaccc gcttggccgt cagcagggac ccgtcgaccc ggaaggtgtg gagcttgtcc
     5341 gacaggttgc tggccgcgcc ggccgccacg ctgccatcga gccgttggct ctcggtgatg
     5401 tacgcgccgt ggagcgtcag gttggtgctg ccgatcggac gctcgtactg ggcgtcgaag
     5461 ccgatgtcgg tatatcggtc ggtcgggccc gagaccccga ccgggaagag cgcggtggac
     5521 atcccgtagg tgccgagctc gaggtagtcg ttcgaccatt ggtgctggac cgcgagccgc
     5581 caatacggcg ctccgccctt gatggtcatc gacgaagtgg agtcaggtgg attcggtccg
     5641 ccctgcgccg ccgaccggta gaagctcgct tcggcgtaca caaggttgtc ccaaaggccg
     5701 tacgccccga ggccggccac ggactggccc aggccgccgt cgatcagcgt ggccgcggcc
     5761 ggggtgggcg ccacggcgga gctcatgtag ggaaagcgcc aggccggcgc ggtgttccac
     5821 acatcctgca ccgtcggatt gttgttgagg gtcaggccgt agaggagctg cttgttggcg
     5881 aaccgggtgt ggtcggcgaa ccgcacatcc aggttgtcca tcccgatggt cccggccgca
     5941 gggtcgtagg tcacctgcat gaacgtgccg aggcggggcg tgatcgcttc accgaagaag
     6001 aggctcagtt gctggggaag ctggacgacc ccgttctgcg tgccggggac gtccttggcc
     6061 gtctggctgt acgacgagac cgccatcgcc gagatcggcg ggatcaggtc gagcttgagc
     6121 gactggcgct ggccctccgc cccggcggtg accacctgca gccccgtgag ggtgtacccg
     6181 ttcagcttga acagccggcc gaacgccgtc aggcgcggga acgcggtatg gcaggcgttg
     6241 caggccagac cggtctggcg ggagaagctc ggaatggccg cgaagtggat ggaggctgtg
     6301 gcccgtcctt cggcgtaccg ccgtgcgtac gtccggaccg cgtcgcccga cccggcggcg
     6361 tggagcgcgg tcgtcgggac cgccagcagg ctcaagacag caaacgttgt gaggtgctgc
     6421 cgcatcagga gatctccggc tcgggtggtg ctggcgccca gggaagggcc gattcgtgaa
     6481 gacgaagctg gtctttcgat tatgaaggaa tcattggggt caagtgagag ggcatttagg
     6541 agcggtgtcc tgcggcgcta aggagtggcg aaggtgcggg tcggggtgcg tgccggggcc
     6601 ggtccgaacg atccgcggga gctcccagcg gcgcccgaag aggcgacatg accgccactt
     6661 ggcaccgacg tgggtggcgg ctacgtttcg ccctcctccg accggtcggt ggagggagca
     6721 cgggacgtag cgcagcccgg tagcgcacct gaatggggtt cagggggtcg cgggttcaaa
     6781 tcccgccgtc ccgatggtgg caagcgaaga tccacgatat cgaacgaagg gccggccctc
     6841 cagggcgggc ccttcgtcgc atttggtgcg gtgaatggac cttcaccggg ccttcacggc
     6901 ttctcaaccc tcgccgtcgc gactccccgt attgttgggc tcgcgggacg gatgggagcg
     6961 gccgtggaac tggatcgcct gatcgagacc gagcagcgca acgatgagct ggtgcggcgc
     7021 gccgcggccg aggcggcggg gatcgtccgc acggcggcg
//
LOCUS       DASUAP010000191         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_291142, whole genome shotgun sequence.
ACCESSION   DASUAP010000191 DASUAP010000000
VERSION     DASUAP010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291142"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFU45_04785"
     CDS             complement(<1..494)
                     /locus_tag="VFU45_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5358409.1"
                     /translation="MIRLDRVGVRAGTFALADVSLTVPTGAHAVLLGPTGSGKTTLLE
                     AIAGHLPLAHGRIELGGADVAQVPPERRRVGVVYQHYHLFPHLSVRDNIGYGLHAAAD
                     APPPARGRVEELAAALGLLHLLDRAPGALSGGERQRVALARALAPGPRILLLDEPFAA
                     ADPA"
     gene            complement(491..1270)
                     /locus_tag="VFU45_04790"
     CDS             complement(491..1270)
                     /locus_tag="VFU45_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5358410.1"
                     /translation="MRPFTPARALLALLGALLVLFIVGPLLKLLVSTSPGGILGALAD
                     PELRESIVLTGITATAATMCGLALGVPLAWLLARTRFPGRRVVDALVDLPVVVPHPVA
                     GIALLLFLGRRSAVGGALAHLGLQFVSHVPGIVAAMLFVSVPIIVSGAREAIAAVDPH
                     FERVARTLGDGPWRTFRRITLPLAGRGIVAAAALGWARAVSEFGAIVILTYHPRVASV
                     LIYDRFTTDGLGAAVPAALILLLVALAVFGVVRALQPDEHR"
     gene            1455..2342
                     /locus_tag="VFU45_04795"
     CDS             1455..2342
                     /locus_tag="VFU45_04795"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5358411.1"
                     /translation="MKIAFLGLGALGAPIAGRLAAAHPTTVWNRSRVRTDEFLARHSA
                     TGASTPREAASGADVVLTCLPTSREVEALLDGPDGLRAGLRPGALLLDCTSGDPATSR
                     RIAARLAEAKVAFADAPVSGGPPAAASGALTVMVGGDAAVFERARPVLAAFGKRIEHV
                     GPVGAGHALKSVNNALLAANILALAEGLTALVKAGVPAGKAVEVINASSGRSFVSESL
                     VPERVLTGTWPRLFRLALLDKDVGIALGVLEDAGVPGPLLGRVRDLTAQLRAELGEAA
                     DYLDPIRRAERQAGVEIRA"
     gene            2412..>2779
                     /locus_tag="VFU45_04800"
     CDS             2412..>2779
                     /locus_tag="VFU45_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358412.1"
                     /translation="MGEWGTTERIEAEILLGDGTRLFGALFVQGRVPHHDGPETLVEM
                     LNRPEPFTAVALTGGGVVFVPKAQIALIAAEPLPAPDDGGARQSTLKLVGLELEMLGG
                     KVLTGLVSVDLPPTHARPID"
BASE COUNT          396 a          980 c         1059 g          344 t
ORIGIN      
        1 atggcggggt cggcggcggc gaagggctcg tcgaggagga ggatccgggg cccgggcgcg
       61 agcgcgcggg ccagcgccac gcgctggcgt tcgcctccgg agagcgcgcc cggcgcgcgg
      121 tcgaggaggt gcaacagccc gagcgcggcg gcgagctcct cgacccgtcc ccgggccggc
      181 ggcggcgcat cggcagcagc gtggagcccg tagccgatgt tgtcgcggac cgacaggtgg
      241 ggaaagaggt ggtagtgctg gtacacgacg ccgacccggc ggcgctccgg tggcacctgc
      301 gcgacatcgg cgccgccgag ctcgatccgt ccatgggcca gggggaggtg ccccgcgatg
      361 gcctcgagca gcgtggtctt gcccgatccc gtgggcccga ggaggacggc gtgcgcgccc
      421 gtcgggaccg tcagcgagac gtcggcgagc gcgaacgtgc ccgcccgcac cccgacccgg
      481 tcgagacgga tcaccggtgc tcgtccggct gcagggcgcg caccacgccg aacaccgcga
      541 gcgccacgag gaggaggatc agcgccgccg gcacggccgc gccgaggccg tcggtggtga
      601 agcggtcgta gatgaggacg ctcgcgaccc tgggatggta ggtgaggatc acgatcgccc
      661 cgaattcgct caccgcccgc gcccacccga gcgcggcggc ggcgacgatg ccgcggcccg
      721 caagcggcag ggtgatgcgc cggaaggtgc gccagggccc gtcccccagg gtacgggcga
      781 cgcgctcgaa gtgcgggtcg acggccgcga tcgcctcgcg ggcgccactc acgatgatcg
      841 gcaccgagac gaagagcatc gccgcgacga tcccgggcac gtggctcacg aactggagcc
      901 ccaggtgcgc caacgccccg cccaccgcgg atcggcgccc gaggaagagg aggagtgcga
      961 tcccggcgac cggatggggg accacgaccg gcaggtccac cagcgcgtcc accacccgtc
     1021 gccccgggaa ccgcgtgcgg gcgaggagcc acgccagggg aacgccgagg gccagtccgc
     1081 acatcgtcgc ggcggtggcg gtgatcccgg tgagcacgat cgactcgcgg agctcggggt
     1141 cggccagcgc gccgaggatc ccgcccggcg aggtggagac gaggagcttg agcagcggcc
     1201 cgacgatgaa gaggaccagg agcgcgccca gaagggccag cagggcgcgc gcgggcgtga
     1261 agggacgcat cgccgggtaa gctgtggcgt tggtgcacgt cggtcaatcg ggtatcgttg
     1321 tgggagcttg gagcttggag cttggagcct ggagctcgga gtctgggatt tcgagctgat
     1381 ggctgatcgc tgaaggctcc cgcctccaag ctccagactc caagctcccc cgcacccgac
     1441 cagggcactc gaccatgaag atcgcattcc tcggactcgg cgcactcggc gctccgatcg
     1501 ccggccggct cgccgcggcc catcccacga ccgtatggaa tcgcagccgc gtgcgcaccg
     1561 atgaattcct ggcgcgacac tcggccaccg gggcgtcgac cccccgggag gcggcgagcg
     1621 gcgctgacgt cgtcctcacc tgcctgccga cctcgcgcga agtggaggcg ctgctcgacg
     1681 gcccggacgg cctgcgtgcg ggactccgtc ccggcgcgct cctcctcgac tgcacctccg
     1741 gagacccggc gacgtcccgg cgcatcgccg cgcggttggc ggaagcgaag gtcgccttcg
     1801 ccgacgcgcc ggtgagcggc ggcccccccg cggcggcgag cggcgcgctc acggtgatgg
     1861 tgggaggcga cgcggccgtc ttcgagcggg cgcgcccggt gctcgcggcg ttcgggaagc
     1921 ggatcgagca cgtggggccg gtcggggcgg ggcacgccct caagtcggtc aacaacgccc
     1981 tcctcgccgc caatatcctg gcactggccg aagggttgac cgccctggtc aaggcggggg
     2041 tgccggcggg gaaggcggtg gaagtcatca acgcatcgag cgggcgttcg ttcgtgagcg
     2101 aatcgctggt gccggagcgg gtgctgaccg gcacatggcc ccggctcttc cgcttggccc
     2161 tcctcgacaa ggacgtcggc atcgcgctcg gcgtgctcga ggatgccggc gttcctggcc
     2221 cgctcctggg ccgggtccgc gacctgaccg cgcaattgcg cgccgagctt ggcgaggcgg
     2281 ccgactacct cgacccgatc cgacgggcgg agcgtcaggc cggcgtcgag atccgcgcgt
     2341 gagccggcgc gcacccttgc cgccgctgcc cgtcggggcg accttttcct cccctccaaa
     2401 cgggcgccgc gatgggtgag tggggcacga ccgaacgcat cgaagccgag atcctgctgg
     2461 gcgacggcac ccgcctgttc ggcgcgctgt tcgtgcaggg gcgggtgccg caccacgacg
     2521 gtcccgagac gctcgtcgag atgctcaacc gtccggagcc gttcaccgcg gtggcgctga
     2581 ccggcggcgg cgtggtgttc gtgcccaagg cgcagatcgc gctcattgcc gccgaacccc
     2641 tgccggcgcc ggacgatggc ggtgcgcgcc agtccaccct caagctcgtc gggctcgagc
     2701 tcgagatgct gggcgggaag gtgctgaccg ggctcgtgtc ggtcgacctg ccccccaccc
     2761 acgcgcggcc catcgattt
//
LOCUS       DASUAP010000192         4501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1122954, whole genome shotgun sequence.
ACCESSION   DASUAP010000192 DASUAP010000000
VERSION     DASUAP010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122954"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2943
                     /locus_tag="VFU45_04805"
     CDS             <1..2943
                     /locus_tag="VFU45_04805"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358413.1"
                     /translation="VWKTDDYGRTWSPIFDGQHTGSIGALAVAPSNPDVIYVGTGEGL
                     QRPDLAVGDGVYKSTDAGRSWRHMGLDGVQQVSAVIVDPVDPERVFVAALGHPYGANP
                     DRGVYRSLDGGATWSKVLYRDENTGAVDLAFDPTDPQVVYAVLWAARQGPWEYDNGYE
                     GPGSGLFKSTDGGTTWRQIGAGLPTPAEGLGRIGIGIAPSDRMRMYALVEAPEGGLFR
                     SDDAGERWVKVDSEERIYGRGADFAAVRVDPRDPNTVFVANTSMYRSTDGGAHFTAVK
                     GAPGGDDYHTIWIDPIHPETMLLGVDQGATITVNGGRTWSSWYNQPTAQMYHVAADDR
                     FPYWVYGGQQESGSAGVASRSDYGEITFRDWHPVGSEEYGYAAPDPLHPGIVFGGKVT
                     RFDERTGQVQEVGPVPVRDGTHRFDRTAPLVFSQADPHLLFFGLEVLWKTADGGRHWQ
                     QVSPDLTRPDPGIPASLGIYAPAAKGGHRGEIYAIAPSPRDVRVIWAGTDDGCIQVTT
                     DGGLHWRNVTPPEMTGWSKVTQLDASHFDAASAYASVSRFRVDDLHPYIYRTHDGGRT
                     WNKVVSGIPDDETVDVVRADPEARGLLYAGTERSVYVSFDDGDHWQPLTLNLPATSVR
                     DLIVHENDLAIATHGRGFWILDDITPLRQAAAANGTAAFLFRPAPAWRWRWDRNTDTP
                     LPPEEPAGQNPPDGAIIDYYLGDSTQGPVTLEIRNSSGQLVRRYSSADTAPTVDTVSL
                     NVPTHWLRPFQPLSAAPGMHRFVWDLHYPPPTALGAEYPIAAIEHDTPRMPRGPVVLP
                     GRYFVRLSVNGKSLEAPLVVRMDPRISTPSAGLAAQFSLAMGLYTAMDRAHDVLAAVR
                     AVRAQVGDRRAHAAGVVADSLTAIDAAITALATSPDGLEPTVGALGGVYGIVEGADAE
                     PTSQAAAAAHRLDRQVAHLEERWVGFRRRLVGVNSQLQATGLAPIDLSSAPGSDARAP
                     EVSHQDEE"
     gene            2985..3596
                     /locus_tag="VFU45_04810"
     CDS             2985..3596
                     /locus_tag="VFU45_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358414.1"
                     /translation="MATPSTLTLRTFPDRAEALSHFLLRAGEAPRIIAYDDAVGCPME
                     MALAALEWTKAVGVLLDDDCLHAARLTVDTAAAVIERRGPGNVRQYIYLGPRMEAPAM
                     DVFEGSVIFDEPGVRAIEFVERSHALAHFLRATSGAGALLSLLSRRAPELRHLRRWLA
                     PALEALDLPRPMVAGWFAASSAGCLFAGVEEPEYRYIEVGMES"
BASE COUNT          689 a         1568 c         1550 g          694 t
ORIGIN      
        1 gtctggaaga cggacgacta cgggcggacc tggtcgccga tcttcgacgg ccagcacacc
       61 ggatccatcg gcgcgctggc ggtggcgccg tcgaaccctg acgtcatcta cgtcgggacg
      121 ggtgaggggc tgcaacgccc cgacctggcc gttggcgacg gcgtgtacaa gtccaccgac
      181 gccggccgga gctggcgcca catgggcctc gatggcgtcc agcaagtatc ggcggtgatc
      241 gtcgatccgg tcgacccgga gcgggtcttc gtcgcggcac tcggccatcc gtacggggcc
      301 aatcccgacc gcggcgtcta ccggtcgctc gacggcggcg ccacctggtc gaaggtcctc
      361 tatcgggatg agaacaccgg tgccgtggac ctcgccttcg atcccaccga tccccaggtg
      421 gtgtacgcgg tgctctgggc cgcgcggcag gggccgtggg aatacgacaa cggatatgag
      481 gggccgggca gcgggctgtt caagtccacc gacggcggta ccacgtggcg gcagatcggc
      541 gcgggcctgc ccactccggc ggagggcctc gggcggatcg gcatcgggat cgcgccgagc
      601 gaccggatgc ggatgtacgc gctggtcgaa gcgccggagg gcgggctctt ccgatcggac
      661 gatgccggcg aacgctgggt caaggtcgat tccgaggagc ggatctacgg gcgcggcgcc
      721 gatttcgccg ccgtgcgggt cgacccgcgg gatccgaaca ccgtcttcgt cgccaacacg
      781 tcgatgtacc gctcgaccga cggcggcgcc cactttaccg ccgtgaaggg cgctccgggg
      841 ggcgacgact accacacgat ctggatcgac ccgatccatc ccgagaccat gctcctcggc
      901 gtggaccagg gtgccaccat caccgtcaac ggcgggcgca cctggagctc gtggtacaac
      961 cagcccaccg cccagatgta ccacgtggcc gccgacgacc ggttcccgta ctgggtgtac
     1021 ggcggacagc aggagagcgg gtcggcgggt gtcgcgagcc ggagcgacta cggcgagatc
     1081 accttccgcg actggcaccc ggtggggagc gaggaatacg gatacgccgc gcccgacccg
     1141 ctgcatccgg ggatcgtctt cggcggcaag gtgacgcgct tcgacgagcg gaccggccag
     1201 gtgcaggagg tcgggccggt gccggtgcgc gatggcaccc accgcttcga ccgcaccgcg
     1261 ccgctggtct tctcccaggc cgatccacac ctcctgttct tcggcctgga ggtgctgtgg
     1321 aagaccgccg acggcggccg ccactggcag caggtgagcc ccgaccttac ccggcccgat
     1381 ccgggcatcc ccgcgagcct cggcatctac gcgccggcgg ccaagggcgg gcaccgcggc
     1441 gagatctacg ccatcgcgcc gtccccccgc gacgtgcggg tcatctgggc cggcaccgac
     1501 gacggctgta tccaggtgac gaccgacggc gggctccact ggcgcaacgt gaccccgccc
     1561 gagatgaccg gctggagcaa ggtgacgcag ctcgacgcct cccacttcga cgccgcgagc
     1621 gcgtatgcct cggtgtcgcg cttccgggtc gacgacctgc acccctacat ctaccgcacc
     1681 cacgacggcg gcaggacgtg gaacaaggtc gtcagcggca tccccgacga cgagacggtg
     1741 gacgtggtcc gcgccgatcc cgaagcgcgc ggcctcctct acgccgggac cgagcgatcg
     1801 gtgtacgtct cgttcgatga cggcgaccac tggcagccgc tcacgctcaa cctcccggcc
     1861 acgtcggtcc gggacctgat cgtgcacgag aacgacctgg ccatcgccac ccacggccgc
     1921 gggttctgga tcctcgacga catcaccccg ctccggcagg ctgcggccgc gaacggcacg
     1981 gcggcgttcc tgttccgtcc tgcgccggcc tggcgctggc gctgggaccg gaacaccgac
     2041 acgcccctgc cccccgagga gccggccgga cagaacccgc ccgacggcgc catcatcgac
     2101 tattacctgg gcgactcgac ccaggggccg gtgacgctcg agatccggaa ctcctccggc
     2161 cagctggtcc gccgctattc cagcgccgac acggcaccga cggtggacac cgtgtcactc
     2221 aacgtgccga cgcactggct gcgtccgttc cagcccctgt cggccgcgcc ggggatgcac
     2281 cggttcgtct gggacctcca ttatccgccg ccgacggcac tcggcgccga gtatcccatc
     2341 gccgccatcg agcacgatac gccgcggatg ccgcgagggc ccgtggtgct tccgggccgg
     2401 tacttcgtgc ggctctcggt caacggcaag agccttgaag cgccgctcgt ggtgcggatg
     2461 gacccccgga tctcgacgcc gtcggccggg ttggccgccc aattctccct ggctatgggc
     2521 ctgtacaccg cgatggaccg cgcacacgac gttctggcgg ctgtgcgtgc cgttcgggcg
     2581 caggtcggcg accggcgggc gcacgcggcc ggcgtggtag ccgattcgct gaccgccatc
     2641 gatgcggcca tcaccgctct cgcgaccagc cccgacggcc tggaacccac ggtcggcgca
     2701 ctgggcgggg tctatggcat cgtcgaaggg gccgacgccg agccgacctc ccaggcggct
     2761 gccgccgcgc accggctcga ccggcaggtg gcacacctcg aggagcgctg ggtcgggttc
     2821 cggcgccgcc ttgtcggggt caactcccag ttgcaggcca cgggtctcgc gccgatcgac
     2881 ctgtcctctg ctccggggtc cgatgcgagg gcaccggagg tgtcgcacca ggacgaggaa
     2941 tgagccggtt agcggatcga gcggggtggc ggtagctttc gaccatggcc accccgtcca
     3001 cgctcacgct ccgcaccttc cccgaccggg cagaggcgct cagccacttc ctgctccggg
     3061 cgggagaggc gccccgtatc atcgcctacg acgacgcagt cggctgtccg atggagatgg
     3121 cgctggccgc gctcgaatgg accaaggcgg tcggtgtgct cctcgatgac gactgcctgc
     3181 acgcggcccg cctgaccgtc gacacggcgg cggcggtcat cgagcggcgc ggtccgggga
     3241 acgtccgcca gtacatctac ctcggcccac ggatggaagc gcccgcaatg gacgtcttcg
     3301 aggggtcggt catcttcgat gagcccggcg tccgcgccat cgagttcgtg gagcggtccc
     3361 atgccctggc gcacttcctc cgtgccacgt ccggtgccgg tgccctgctg tcactgcttt
     3421 cgcgccgggc gccggagctt cgccacctcc ggcggtggct ggcgcccgcc ctcgaggcgc
     3481 ttgacctccc gcgcccgatg gtagccgggt ggttcgccgc cagctccgcg ggctgcctct
     3541 ttgccggggt cgaggagccg gagtaccggt atatcgaggt cgggatggag tcgtgagcgg
     3601 gtgagacgta agacgtgaga cgtagagagt gagacgtggc tccggtcgag atcggagccc
     3661 cgtttgcgtt caggggtctc gatagcgtca tctgagtccg gcgaggagac caaggtcagg
     3721 tcctcccact cgtcattgag cagcgttgct ccggcccgta attcgttcgc cacgtgccaa
     3781 catccggtgg ttttgttaca cagcgtgctg gtttgtgggc tggggatatc tggaacagtg
     3841 tggatgcaca tctccacaat tggtggagaa cctcgttaca tcgctcaatc cacagcgttt
     3901 acgtatcaac atctgtccac tttgaagtgg cgtaagccga agaaagacag gcaattgcgg
     3961 gcttttcacc cgtccctatc ttgggcacac cgctggcggg gaacgatcca tgacctccgg
     4021 tcgtggatgc cgcctcccag cgagctcggg tgggatatca ggggagatcg ccgacgcacc
     4081 aagggctcag gcgatcgtcg atcctggagg ctctccggcc cgggcagagg ggaccagtcg
     4141 cgcgtcccgt ccaccccgat cttgtgcctc ggggtgcagt gcgaaggggt ggtccgcgac
     4201 ccgctccgac tcgcagcact cgcggtgatg aaccaggtgg gaccgatcat ccgacctggg
     4261 gatggaagcc gaggcccctg ctggccctcc cgagcggcgg aacagccaac cggtggcggc
     4321 agggagaaga acggcggggc aacccccgac ctcgggtagt cgccgcgtgg gacggccgct
     4381 tggtgaagaa ccaggcggcc gtccttgttt ttcggggaac ggtgaacggt gaacagtgaa
     4441 cgggaaacgg ggtgcgccgt accgctgccc gttcaccgtt caccgcttac cccctcaccc
     4501 c
//
LOCUS       DASUAP010000193         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_700373, whole genome shotgun sequence.
ACCESSION   DASUAP010000193 DASUAP010000000
VERSION     DASUAP010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700373"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /gene="tatC"
                     /locus_tag="VFU45_04815"
     CDS             complement(<1..909)
                     /gene="tatC"
                     /locus_tag="VFU45_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5358415.1"
                     /translation="MTGSSGEMSFLEHLEELRTRIIRSLLAVVGGFGIGAWAVDRFDL
                     INVIKRPIAPYIPAGKLVILSPTEPVIIVLKLSLYVGLVLASPVILYQIWAFLSPALY
                     AKERRLLIPSLVAGVVLFFVGAALGYAFVVPPSLKMLLSFEANSFTNMITYQEYFSFV
                     VHLVLALGISFELPLVIIILASVGLVTPRTLNRFRKMAIILAMVAGAALSPSPDVVTM
                     LIMTAPLLVLYEIGFLGTVIVSRRRARRAAATAAVLLAILAPLSAPGRLAAQVPGRAG
                     GVVAPRRAVPGDTGAADTTRGRRPGQP"
     gene            complement(928..1734)
                     /gene="ybgF"
                     /locus_tag="VFU45_04820"
     CDS             complement(928..1734)
                     /gene="ybgF"
                     /locus_tag="VFU45_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEU5358416.1"
                     /translation="MIRGTRPILLAVLLGGCAAKGDVRRVEDAVMLLRAETVRQDSVR
                     AAALAEVIATQQRILDSIAATRSQLTSLKGDVAGDLYNINQQLVQVQELTGQSQQRLT
                     ELRTQLEARSAEMQAAAPAPGAAAGGADSGAAPAVPPGGAPSAPPDQMYDASLQQLRL
                     GSVRTARMGFSQFLQQYPQNARAGDATYFIGESWAAESPDSAARYYDLVVKNFAASPR
                     AATALYKLGLLAENRKDIAGARTYYQRVVQSYPRSDEAGLARDRLKTLGH"
     gene            complement(1731..2354)
                     /locus_tag="VFU45_04825"
     CDS             complement(1731..2354)
                     /locus_tag="VFU45_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU5358417.1"
                     /translation="MRRSTLSLAAVLAAAALVAACGSSPKPEQPAPQPTQPAINADSA
                     RAAQDAAARQAQQEAAQRAREQAQADSAAAASRAAAASAEVASALATMIHFDFNKSDI
                     KSDDQATLDYKAKILQANSALTIRVNGYADERGSDEYNLALGMRRATAAKRYLVNKGV
                     ADARVTTFSYGEDHPIDPGHNEDAWAKNRRDEFQIMAGGTSLVAPGQ"
     gene            complement(2370..3677)
                     /locus_tag="VFU45_04830"
     CDS             complement(2370..3677)
                     /locus_tag="VFU45_04830"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEU5358418.1"
                     /translation="MRRAAGRLALVGAAALGLLPGRLRAQDTSHVEGVRVGITYAAGI
                     RPGLLVLWAPGLDSVGAIIQRDLDYSDQFEMIPGAVNPHDGPVNDGLYRGMGAAYAVE
                     VTGLTGAVDIRLHDLVAKQVRQDRVAVLPGVGAPDFRMAVHRVADEIVRWITGQPGIA
                     ATQILYDEGSQVFRMDADGAARVAVTRPGERALSPAWSPDGTRMAFTALGDGVAPVIV
                     QTLATGARVTVPGTATGENITPVFTPDGQGVAYAKASEAGTDLYVANVAQMCCVQRLT
                     VGRYSDNLSPTFSPDGQRVAFVSNRPGVPQVYVMAPDGTGQELFAPFDFGATGDSYAP
                     DWSPDGASVVFHREVSGTPQVFVLDVASRKVRQLTSAGRNEDASWAPDGRHVVFVSDR
                     SGRRQLWVVDVESGRFRELATAAAARLPAWSRRLAAAAPVFTR"
     gene            complement(3674..>4096)
                     /locus_tag="VFU45_04835"
     CDS             complement(3674..>4096)
                     /locus_tag="VFU45_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HEU5358419.1"
                     /translation="APKPTPSKAEAPVHKAPPPPATAAPGETPSTGQDAITVKTPGLD
                     FPFPDYLRNIVSQVYRRWDRPAGQGGLRAEVFFLITRDGTVKDIRFITSSGNFAFDLG
                     AQGAVEAAANARAFGRLPDGYGQDVLPVSFYFVPGQGR"
BASE COUNT          601 a         1455 c         1491 g          549 t
ORIGIN      
        1 gggctgcccg ggccggcggc cgcgggtggt gtccgcagcg ccggtgtccc ccgggaccgc
       61 ccgacgcggt gccaccaccc cgcccgcgcg ccccgggacc tgcgccgcga ggcgcccggg
      121 cgccgagagc ggcgccagga tggccagcag cacggccgcg gtcgcggcgg cgcggcgcgc
      181 gcggcgccgg ctcacgatca ccgtgcccag gaacccgatc tcgtagagca ccaggagcgg
      241 cgccgtcatg atgagcatcg tgacgacgtc gggactgggc gagagcgccg cgcccgccac
      301 catggccagg atgatggcca tcttgcggaa ccggttgagc gtccgcggcg tcaccagccc
      361 gacgctcgcc aggatgatga tgaccagcgg cagctcgaac gagatgccca gcgccagcac
      421 caggtgcacc acgaagctga agtactcctg gtacgtgatc atgttcgtga acgagttcgc
      481 ctcgaagctg agcagcatct tgaggctggg cggcaccacg aacgcgtacc ccagggccgc
      541 gccgacgaag aagagcacca ccccggccac gagcgacggg atgaggagcc gccgctcctt
      601 ggcgtagagc gcgggcgaga ggaacgccca tatctggtag aggatgaccg gggaggcgag
      661 gaccaacccg acgtagagcg acagcttgag gacgatgatg accggctcgg tcgggctcag
      721 gatgacgagc ttgccggccg ggatgtacgg cgcgatgggg cgcttgatga cgttgatgag
      781 gtcgaaccgg tcgaccgccc aggcgccgat gccgaagccg cccacgacgg ccaacagcga
      841 gcggatgatg cgtgtccgga gctcctcgag gtgctcgagg aaggacatct caccggagga
      901 cccggtcatg ggcgcgcccc ggggggccta gtggcccagg gtcttgaggc ggtcgcgggc
      961 gaggccggcc tcgtccgagc gcggatagga ctgcaccacg cgctggtagt acgtgcgggc
     1021 gcccgcgatg tccttccggt tctcggccag gagcccgagc ttgtagagcg ccgtggcggc
     1081 gcggggggag gccgcgaagt tcttcacgac caggtcatag taccgcgcgg cggagtcggg
     1141 agactcggcc gcccacgact cgccgatgaa atacgtggca tcgccggcgc gggcgttctg
     1201 cgggtactgc tggaggaact ggctgaagcc catccgcgcg gtgcggacgc tgccgagccg
     1261 cagctgctgc agcgaagcat cgtacatctg gtcgggcggc gcggacggcg ccccgcccgg
     1321 cgggaccgcg ggcgccgccc ccgaatcggc cccgcccgcc gcggcgccgg gtgccggcgc
     1381 agcggcctgc atctcggcgc tccgggcctc gagctgggtg cgcagttcgg tcagccgctg
     1441 ctggctctgg ccggtgagtt cctgcacctg gaccagctgc tgattgatgt tgtacaggtc
     1501 cccggccacg tcgcccttga gcgacgtgag ctgggaccgg gtggcggcga tggaatcgag
     1561 gatgcgctgc tgggtcgcga tgacctccgc cagcgccgcc gcccgcaccg aatcctgccg
     1621 gaccgtctcg gcccggagca gcatgaccgc atcctccaca cgccggacgt cccccttggc
     1681 ggcacagcca ccaaggagga cggccagcag gatgggccgg gtgccgcgga tcactgcccc
     1741 ggcgccacca gcgacgtgcc gccggccatg atctggaact catcgcgccg gttcttggcc
     1801 cacgcatcct cgttgtggcc cgggtcgatc gggtgatcct cgccgtagga gaaggtggtc
     1861 acccgcgcgt cggccacgcc cttgttgacg aggtaccgct tggccgccgt ggcccggcgc
     1921 atcccgagcg ccaggttgta ctcgtcggac ccgcgctcat cggcgtaccc gttgacccgg
     1981 atggtgagcg ccgagttggc ctggaggatc ttggccttgt agtcgagcgt cgcctggtcg
     2041 tcggacttga tatccgactt gttgaagtcg aagtggatca tcgtggcgag ggcgctggcc
     2101 acctcggccg aggccgcggc ggcgcgcgac gcggcggcgg cggagtcggc ctgcgcctgc
     2161 tcacgggcgc gctgggccgc ctcctgctgg gcctggcggg cggcggcgtc ctgggcggcg
     2221 cgcgccgagt cggcgttgat ggccggctgg gtgggctgcg gtgcgggctg ctccggcttg
     2281 gggctggaac cgcaggccgc gaccagcgcg gcggccgcca ggacggcggc aagggaaagc
     2341 gtggaacgac gcatctggga tctcctggct tatctggtga ataccggcgc cgcagctgca
     2401 agtcggcggg accacgccgg gagccgggcg gcggcggcgg tcgcaagctc gcggaaccgg
     2461 ccggactcga catccacgac ccagagctgg cggcggccac tccggtccga cacgaacacc
     2521 acgtggcgcc cgtccggcgc ccacgacgca tcttcattgc ggcccgccga ggtcaactgc
     2581 cgcaccttcc ggctggccac atcgagcacg aacacctgcg gcgtcccgct cacctcgcgg
     2641 tggaacacca cgctcgcccc gtcgggcgac cagtccggcg cgtaggaatc gccggtcgcg
     2701 ccgaaatcga atggcgcgaa gagctcctgg ccggtgccgt cgggcgccat cacgtacacc
     2761 tgcggcacgc ccggccggtt cgagacgaac gccacccgct ggccatcggg cgagaacgtg
     2821 ggtgacaggt tatccgagta ccgccccaca gtcaagcgct ggacgcagca catttgcgcg
     2881 acatttgcga cgtacaggtc ggtcccggcc tccgacgcct tggcatacgc gaccccctgc
     2941 ccgtccggcg tgaagaccgg ggtgatgttc tccccggtcg cggtgccggg gacggtgacg
     3001 cgggcgccgg tcgccagggt ctggacgatg accggggcca ccccgtcccc gagcgcggtg
     3061 aaggccatcc gggtgccatc cggcgaccac gccggcgaga gcgcccgctc cccgggccgg
     3121 gtcacggcga cccgcgccgc cccgtcggca tccatccgga acacctgcga cccttcatca
     3181 taaaggatct gcgtggcggc gatccccggt tgcccggtga tccaccggac gatctcatcg
     3241 gccacccgat gcaccgccat ccggaagtcg ggcgccccca cccccggcag caccgccacc
     3301 cggtcctgcc gcacctgctt ggccaccagg tcgtgcagcc ggatgtccac cgcgccggtc
     3361 aggccggtga cctccaccgc gtacgccgcg cccatcccgc ggtagagccc gtcgttgacc
     3421 ggcccgtcgt ggggattgac ggcccccggg atcatctcga actggtcgct gtagtcgaga
     3481 tcgcgctgga tgatggcgcc caccgagtcg agcccgggcg cccacagcac caggaggcct
     3541 gggcggatcc cggcagcgta ggtgatgccg acccgcaccc cttcgacgtg ggaggtgtcc
     3601 tgcgcccgga gcctccccgg aagcagaccg agcgccgccg cgcccacgag ggcgagccgg
     3661 cccgccgccc gcctcaccgg ccctgccccg gcacgaagta gaagctcacc ggcagcacgt
     3721 cctggccgta cccgtcgggg agccgaccga acgcgcgcgc gttggcggcg gcctcgacgg
     3781 cgccctgcgc gccgaggtcg aacgcgaagt tgcccgacga agtgatgaag cgtatgtcct
     3841 tcacggtgcc atcccgggtg atcaggaaga agacctcggc ccgcagccca ccctggcccg
     3901 cgggccggtc ccagcggcga tagacctgcg agacgatgtt gcgaaggtag tccggaaagg
     3961 ggaaatcgag ccccggcgtc ttgacggtga tcgcgtcctg gccggtgctc ggcgtctctc
     4021 ccggcgccgc ggtggcgggc ggtggcggcg ccttgtggac cggcgcttcg gccttggacg
     4081 gcgtgggctt gggcgc
//
LOCUS       DASUAP010000194         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_319079, whole genome shotgun sequence.
ACCESSION   DASUAP010000194 DASUAP010000000
VERSION     DASUAP010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319079"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..928)
                     /locus_tag="VFU45_04840"
     CDS             complement(41..928)
                     /locus_tag="VFU45_04840"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5358420.1"
                     /translation="MSSTLTAPSPIAARGYAHPEVLVDTVWVADHLMDPTVRLVESNE
                     DLLLYDTGHIPGAVKLDWLTDLNDPTVRDYVDRAALQRFLRGIGVSADTTVVLYGDKN
                     NWWATYAFWVLRLFGLERLRILDGGRARWAEEGRPLVTERPRYPAGTITVGERNDAAI
                     RAFRDEVVAHVRAGKKLVDVRSPEEYHGERLHMPDYPNEGAVRGGHIPGARNIPWGKA
                     VDPASHTFKSAADLRVLYEQEQGLSAEDEVVAYCRIGERSSHSWFVLTYLLGYPRVRN
                     YDGSWTEWGNGVGLPIERP"
     gene            1128..2081
                     /locus_tag="VFU45_04845"
     CDS             1128..2081
                     /locus_tag="VFU45_04845"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5358421.1"
                     /translation="MRSWLRLERRVQGFSPLDRLTVVYVACTTGILLAHIAGWRQERP
                     TPVEAGWLLAANALLCVAVLVAPRARAVRKHSFLAEWYVPFVVSALYAAIGLLNGSPL
                     VVDTSFDHVVQRWEFVIFGQQLAYTWGRVMPSVALSWFLGLCYLAFYFVVIIGPAVLW
                     AVGRRAASRQAIYAIVLSFFACYLVFLLFPVGGPPYFWTWPTGPGNDAWPVRVARHII
                     EGQDSFGTAFPSSHVAAALTAAIFAYRGWRPLGLVLLVPALGIIAAVVYLQIHYGVDA
                     LAGILVAILVMLVTPRLCPATAPLGHAEMCDAGDLDAAEGA"
     gene            2178..2621
                     /locus_tag="VFU45_04850"
     CDS             2178..2621
                     /locus_tag="VFU45_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5358422.1"
                     /translation="MKLMRTTPSIATAVRDDFDRLVDRLFTTPLLPAWSEQPATEWAP
                     SLDFSETDKEYIVRVEAPGVPKDDIDVNLEGQVLTISGHRETSHEEKTEEYFWKERSA
                     GRFLRSVRLPSAVKMEGVDAKCSDGVITVKVPKAETAIKNRITVK"
BASE COUNT          382 a          954 c          956 g          456 t
ORIGIN      
        1 cgatctgaac tctcagccct gagcgctcag ccctcgccga tcagggcctc tcgatcggca
       61 ggccgacgcc gttcccccac tcggtccacg acccgtcgta gttccgcacg cggggatagc
      121 cgagcaggta ggtgaggacg aaccagctgt gggaggagcg ctcgccgatg cggcagtagg
      181 cgacgacctc gtcttcggcg gagagtccct gctcctgttc gtagagcacg cggaggtcgg
      241 cggcggactt gaacgtgtgg gaggcggggt cgaccgcctt gccccacggg atgttgcggg
      301 cgccggggat atggccaccg cgcaccgcgc cctcgttcgg gtaatcgggc atgtggagcc
      361 gctcgccgtg gtactcctcg gggctccgca cgtcgaccag cttcttgccg gcgcgcacgt
      421 gggccacgac ctcgtcgcga aaggcgcgga tcgcggcatc gttccgctca ccgacggtga
      481 tcgtcccggc cggatagcgg ggacgctcgg tcaccagcgg cctgccctcc tccgcccacc
      541 gcgcccgccc gccgtcgagg atgcggagcc gctcgagccc gaagagccgc agcacccaga
      601 aggcgtaggt ggcccaccag ttgttcttgt cgccatagag cacgacggtc gtgtcggcgc
      661 tcacgccgat cccgcggagg aaccgctgca gggcagcacg gtccacgtag tcccgcacgg
      721 tgggatcgtt caggtcggtg agccagtcga gcttgacggc gccggggatg tggccggtgt
      781 cgtagaggag gaggtcctca ttcgactcga ccaggcgcac cgtcgggtcc atcaggtgat
      841 cggcgaccca gacggtgtcg accaggactt cgggatgggc gtagccgcgg gcggcgatcg
      901 gtgagggggc ggtgagggtg ctgctcatgg tgggtcccaa gcgtgggggg actgcaatta
      961 cgactctttt agtcggcttg tcaatcatcg cgcggcgggg ggcccgccgc tagattttcg
     1021 gtttcgcatc ccggggccgg cgcccgggct ggtgccgccg ggcgggccgg cgcgaccgac
     1081 gaccgttgca aggagacggc acgagcgtgg cacctcccgc agtcctattg cgcagctggc
     1141 tccggctcga acgccgggtg caggggttct cccccctcga tcggctgacc gtcgtctacg
     1201 tcgcctgcac caccgggatc ctcctggcgc acatcgccgg gtggcggcag gagcgtccga
     1261 cgccggtgga agcggggtgg ctgctcgcgg ccaacgcgct cctctgcgtg gcggtgctgg
     1321 tggcgccccg cgcccgcgcg gtccgcaagc attccttcct ggcggagtgg tacgttccct
     1381 tcgtcgtgag cgcgctgtac gcggcgatcg gcctcctcaa cggctcgccg ctcgtggtcg
     1441 acacgtcgtt cgaccacgtg gtgcagcgct gggagttcgt gatcttcggc cagcagctgg
     1501 cgtacacctg gggccgggtc atgcccagcg tggcgctctc ctggttcctc gggctctgct
     1561 acctggcctt ctatttcgtg gtgatcatcg ggccggcggt gctgtgggcg gtgggccggc
     1621 gggcggcgtc ccgccaggcg atctacgcca tcgtgctgtc gttcttcgcc tgctacctcg
     1681 tattcctcct cttcccggtc ggcggcccgc cctatttctg gacctggccc acggggccgg
     1741 ggaacgacgc ctggccggtg cgggtggccc gccacatcat cgagggccag gattcgttcg
     1801 gcaccgcctt cccgtcgagc cacgtggcgg ccgcgctcac cgcggcgatc ttcgcctacc
     1861 ggggctggcg ccccctcggc ctcgtgctcc tggtgccggc gctcggcatc atcgctgcgg
     1921 tggtctacct ccagatccat tacggcgtcg acgcgctcgc cgggatcctc gtcgcgatcc
     1981 tggtgatgct cgtgacgccg cgcctgtgcc ccgccaccgc tcccctcggg cacgccgaga
     2041 tgtgcgacgc cggcgacctc gacgccgccg aaggcgcgta agcaaagttt ctcgcccagt
     2101 tcaccgccga ttcaccgctc ccgccgcatc ttccaccttg ctcgcagtcc ggtccatacc
     2161 gcagcaagga gggaagtatg aagctcatgc gcaccacccc cagcatcgcc accgcggtcc
     2221 gggacgattt cgatcgtctc gtcgaccgcc tgttcaccac cccgctcctg cccgcgtgga
     2281 gtgaacagcc ggcgaccgaa tgggcgccga gtctcgattt ctccgagacc gacaaggagt
     2341 acatcgtccg ggtcgaagcg cccggcgtcc cgaaggacga catcgatgtg aacctcgagg
     2401 ggcaggtcct cacgatctcc ggccaccggg agacgtcgca cgaggagaag accgaggagt
     2461 acttctggaa ggagcgctcg gccggccgct tcctgcggag cgtgcggctg ccatcggcgg
     2521 tcaagatgga gggcgtggac gccaagtgct ccgacggcgt gatcaccgtg aaggtcccga
     2581 aggctgagac cgcgatcaag aaccgcatca cggtgaagta gcgctcaccg ccgggcgtgg
     2641 agcgaccgcc gcagctggac gagggaacgg gcgttggcaa catccgggac ctcggcccag
     2701 ccgcggcggg cctgcgtcac cccccagcgc aggaagccga gttcggcg
//
LOCUS       DASUAP010000195        22461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_905096, whole genome shotgun sequence.
ACCESSION   DASUAP010000195 DASUAP010000000
VERSION     DASUAP010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 22461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22461
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905096"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFU45_04855"
     CDS             complement(<1..574)
                     /locus_tag="VFU45_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5358423.1"
                     /translation="MDIRNIAIIAHVDHGKTTLVDQMLRQAGAFRANQHVEERVMDSN
                     PLERERGITILAKNTAVHWGGTKINIVDTPGHADFGGEVERILRMVDGVLILVDAAEG
                     PMPQTRFVTRKALALGLRPIVAINKIDRQDAEPFRVHDEVLGLFIDLEASPEQLDAPF
                     LYTSSRAGTATTELEHPGTDLKPLFQAILDH"
     gene            complement(721..1686)
                     /locus_tag="VFU45_04860"
     CDS             complement(721..1686)
                     /locus_tag="VFU45_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU5358424.1"
                     /translation="MQPTPSTPALCLIRVPRTYTRRLWWVLALTVVICGAEVAGAVIS
                     GSLALLADAGHMLADVAAIGLSLFTAWIAERPADATRTYGYLRWEILGAFINGAALFV
                     IAGWIIAEAVGRLHAPPPVQGKVLFGVALLGLAANLGSLFTLHGGHTENLNVRAAYLH
                     VLGDALGSIGALLAGVIILTSGWTLVDPIVSVGIALLVLVGAWRLVRESVDILLEGTP
                     SHISLAEVEGRMRALPGVTAVHDLHVWTVTSGLVAMSGHAVVPDLGAHPEALTGIQRA
                     MAALGIGHVTIQLETGGPCAEAEVAGPAAGHAHGHHHSHAGHHHH"
     gene            1988..4798
                     /locus_tag="VFU45_04865"
     CDS             1988..4798
                     /locus_tag="VFU45_04865"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358425.1"
                     /translation="MSGALLVTPGLRPAWLSALEDSRTAAVARGAAGPVDPALFGGLS
                     WRMLGPFRGGRTDAVSGVPGRPEEFYFGHVNGGVWKTNDGGRTWRPVFDGQPVASIGA
                     LAVAPSAPDVLYVGSGESTLRDSVGYGNGMYKSTDAGRTWTHIGLDDTQHIGKIAVHP
                     KDPDLVFVAALGHYYGPSKERGVFRSKDGGKSWDKVLYKDENVGAIEVAIDPNDPRVV
                     YAGLWNTRRPPWFTYPPTNGPGGGIFKSTDGGDTWTQLTHGLPSEGIGRTGIAVAPSD
                     SKRVYAVVDCLVPEPGAAVPPPDPSGFGRDTTPRQGGFFRSDDGGATWIRLSADQALW
                     GRGWYFEHITVDPTDAEVVYVANVSVSRSKDGGHTWVPLRGSPGGDDYHQPWVSPDDP
                     NTLIVASDQGTVITRNAKADDPRQVTWTSWLNQPTAQIYHVSVDYRFPYWVTGAQQDS
                     GAVAVCSRGKFATISMRDWEPIGAGGESGATAGDPLHPGIIFGESGTRFDLALNREIP
                     GTTAPEAADKTPLRRDWTQPLVFSPADPHALYFAAQFLFRSTDGARTWTQVSGDLTRT
                     APGVPRNLDAAAAADVQQNGQRGVIYTVAPSPLQAPLLWIGTDDGVIQVSPDGGDHWR
                     DVTPPALSAWSRVTTVEASHFDPAVAYASVDRHQLEDFEPYIYRTRDQGKHWQKITDG
                     LPPGVYVHVVREDPVRRGLLFAGTERGAFVSFDDGDHWQPFQLDLPVTSVRDFQVYQN
                     DLIVATHGRGFWVIDDISPLRQVGAEVASADAWLFQPADAIDVIVGTDNGTPLQKDEP
                     QAESPPDGAYLYYYLKADAPAPVTLEILDAAGATLHTFSSAATTAPRGNGAPGIPNTT
                     ALWREPAAPFSAAAGLHRAVWKPNTTPRPGFGGRRRPQDPVVSLPGTFTARLTVAGKS
                     QTRTFHVRPDPRATAEDTSLGT"
     gene            4835..6151
                     /locus_tag="VFU45_04870"
     CDS             4835..6151
                     /locus_tag="VFU45_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5358426.1"
                     /translation="MNDATGGSTLFGLVSVIALVLANAFFVAAEFAMVGIRRSRIEEL
                     AQGGVGSAVLVRRALTSLDRYISATQLGITLASLGLGWVGEPALAHLFERWFTGLPAA
                     LRPWATHGTAATVAFLLITGLHIVLGELVPKALAIAYPERVTGVTIRPLSAFVWAMTG
                     PIAVLNGAAWQILRLLGLRRRSSQAPLHSPEEIRILVEQSQQAGSLGTEDARLLEGVF
                     EFSEKNAEQVMTPRTQMTALEAGLPVEEAAAIVAETGRSRYPVYEGSLDDVVGVVHAK
                     AILAALRQDPHQPVRNVMRPPLFVPGSREVEDVLADMKRVKSHLALVLDEYGGTAGLV
                     TMEDLLEEIVGPIEDEYDRPVRTPAPTGGALELDGAMPIAEANERLDLSLDDADYTTL
                     GGYLFGQLGRLPAVGDRVRAEGREFEIAEMEGRRVKRVRVVGRSEK"
     gene            6384..6773
                     /locus_tag="VFU45_04875"
     CDS             6384..6773
                     /locus_tag="VFU45_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU5358427.1"
                     /translation="MQRHTSLRAWQTARSVALGVFRANASHWRPWVAPAFEQVGRAAL
                     SVQLNIAEGYSFGRSASFTNHLRIAYGSAVETADLLEFLRELGAVTAAECDRLIALNR
                     ECQACIAGLLRNRNSWARPASQADGRE"
     gene            complement(6909..7823)
                     /gene="pdxA"
                     /locus_tag="VFU45_04880"
     CDS             complement(6909..7823)
                     /gene="pdxA"
                     /locus_tag="VFU45_04880"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU5358428.1"
                     /translation="MTRRIAVTLGDPRGIGPEIVSRALATPLDALVTLVGAEDQLAGL
                     PAARRVSVGHWGAGSGEGAADRPRAVRAGMIAGHAVEAAARLALAGEVDAIVTAPIAK
                     HAIRLAGFPFPGHTEWLAHLAGDVPVAMMLAADRLRVVLVTTHVPFRDVPVLLTTDKI
                     VRVGQVTLTALRDWFGIAAPRLALCAVNPHAGEQGLFGDEEARILAPAARILGAAGPL
                     PADTVFVRAMRGEFDAVLAPYHDVGMTAIKVAAFGEGVNITLGLPFPRTSPDHGTAFD
                     IAGQGKADATSMRRAMELACELAERSKK"
     gene            complement(7820..9142)
                     /locus_tag="VFU45_04885"
     CDS             complement(7820..9142)
                     /locus_tag="VFU45_04885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5358429.1"
                     /translation="MGRAFAAAALASTLLLGPALRAQAPGDTGESVDHVVAVVGNVPI
                     LNSQVEERIFSNQQAGQPVPTDPDSLRKLRRQVLSDLVDEELLVQQAERDTTIHVTDQ
                     DVSDAVDQRIRQIRTSFATELDYKNELKKAGFISPEEYRRWLTDQQRRTLLQNHLVSS
                     LFDDQKLKPVTPTERELRDYFNATPDKGTRKAAVAFKQVVVAPRASPAARAVAHATAD
                     SIAKALRAGADFATAARRFSQDPGTKDQGGELGWFRRGQMVRQFDDAAFRLKPGVISD
                     PVESPFGFHVIQVERVQPTEVEARHILITPAVTAAEADSARQLAERLRGQVMAGASFD
                     SLQRIYSDSSEERSFDLVPLDQLPPAYHPVMQADSGQVTDIFELPNPVDSLRAKWAFA
                     LVTGKRGEGPMTFDEVKDQLRPQLAKQLAVQRYIARLRRDNYVEVRGP"
     gene            complement(9142..10626)
                     /locus_tag="VFU45_04890"
     CDS             complement(9142..10626)
                     /locus_tag="VFU45_04890"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5358430.1"
                     /translation="MRRNTLCLGLGLLLVAGCRGGVGKLFSAHAKEAASAGPISLTPE
                     SLAVTLAGLKGAQLAPETADFVANLWINYHLFALAVADGKIATDSAAVAEVMWPEMTE
                     RIAGQWHDTVVARHANFTAHSVDSAYASTDTMALRLVQHILITVPASAPDSVKAKARK
                     KVEGLAAQIRAGANFGALALKFSGDPGSARDSGYMQPAPRGAYVTSFDSAAWTLAPGA
                     VSGIVATPYGFHIVRRPPVNEIRDRFQAFLNQRAGMVLDQMYFDSLAKAKHLKVASKA
                     PIKLRELLTDPESYRHSSEAFASYDGGKLEGREMVRWVNVLPPQIASGLGQATDSQIS
                     NFISRVAENVLFVQDAKAHGIKVSAADMETMRQAYIAGVDTLRAKMGLGDDLMNPATP
                     LADREKAAALKVDQFLAYGFGARVGMTPIPAPMVDYLRDRFPSHVDQQGTARAVELAL
                     NIRAKAEMEQDSTARAHGKAPAPSPEHNNAAPVTLPTTPAPAPR"
     gene            complement(10627..13989)
                     /gene="mfd"
                     /locus_tag="VFU45_04895"
     CDS             complement(10627..13989)
                     /gene="mfd"
                     /locus_tag="VFU45_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU5358431.1"
                     /translation="MTARPPAITTPKSGPLDLIVDAFDRTRGAYELGPRLPARGERLV
                     LGGLPGSSPAVLAASLARAHPARLIAVVAPGPAEAERWLTDLKHLTELPVGLYPQRES
                     LGEEESHYEIAGERAETLDDLAHGRIRIVVTTARATAERTPMAAALETARLTLRAPED
                     GKGLAEGPALGAVTERLESMGYQRVPTVSAVAEYSVRGGIVDVYGFGMAQPVRAEWWG
                     DDLIGLRAFDLTTQRSQGALADVTILPVRAPARSAPGPKGAGSEVPAVRRSLLELLPA
                     DALIIQEREGPDAEEVGRAWRDAEHHLAVARRLGDEAPAREEVLESPDRWMRSLGRFA
                     RLTVRDAKPDLDMGFLPPERVDRDLGRLRAILAGELPSVILCDNDGQRERLDDLLSEG
                     ARTVNAALKVGALDGGFVMSTLRVLTDHEIFRRARRLRRARRYRTAAPSSATGALASG
                     DYVVHLDHGIGIYRGIETITIGAGTLEVAVVEYAGGTRVNVPLYRLDLLEHYRAAGDD
                     GDRPPPKLHTIGGSSWQKTRDRTRAAIRQMAGELVDLYARRKVTAGFAFPPDTRMQRE
                     LESGFLYEDTPDQRKATEDVKRDMELPRPMDRLLVGDVGYGKTEVAVRAAFKAVQGGK
                     QVAVLVPTTILAEQHGRTFQERMADFPVRLEVLSRFRTAREQAAALARLTTGETDIVI
                     GTHRLLSPDVAFQDVGLLIVDEEHRFGVKHKERIKALKLAVDVLTMTATPIPRTLHLS
                     LTGLRDLTLIETAPRDRSPVLTFVEPWDDALLEEAFARELDRGGQVFVVHNRIETIAT
                     IAQRVRALAPRARVGVAHGQQGAEELEGVMEAFVRGELDILVSTMIVESGLDVPNANT
                     MVVHDAHRFGLAQLYQLRGRVGRSHRRAYCYLLAPDELDLMAQERLKVLEHHTELGAG
                     YRIAVKDLEIRGAGNLLGAEQSGHAHAVGFDLYLRWLEETVTALHGQARGAEAPVPPE
                     VAIDQPSHLPDRYVPDDDAKLDIYRRLARAVSSGEIDALDDELRDRFGPVPAPAAVLL
                     DVARLRVLGARLGLQNVLVQGDEARLSFRAGAAPRLTGLTRALDDVQLAAEVRRTVPL
                     ALRLTRLGGEPLVPALVRALRAVHATVHSQGD"
     gene            complement(13992..15797)
                     /locus_tag="VFU45_04900"
     CDS             complement(13992..15797)
                     /locus_tag="VFU45_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5358432.1"
                     /translation="MIGFLYPWALAGLAAAAIPLLLHFRAQREPPTVVFPAVRYLRDA
                     TRQHERRLLLRQWLLLLLRTLLVVALVLAAAGPTVPLGSAGPHAPMALVLVLDNSLSS
                     GVTVGGTPRLAGLRRAALDVLARAGAGDRLWLLTADGIPRRGDPLFLRRVVDSLQPSG
                     MRMDLGAAVDVAGRIVDGAGLPGGVVVLSDLQGSALSAAATRQPVLVGAPSAAGAPPP
                     NLGVASVLPGAEPWSPDGGTVTVTVAGDHDSTPVPVTITLGAGPPRQALVGPGRPAVV
                     TLPGGRAGWSALTARLDPDELRGDDARTVAVRVAPTPRVAWSASDRYVAAALATLVAG
                     RRAVAGDGLRVGSLGAGPSVVFPPADPALVGALDRALAARGVAWRYGPLLDDTAAIDS
                     SAWLRPARVWRRYRLVPNGSGRTGVLITVGGEPWLVRSGPVLLLGSRLEPAWTELPLV
                     AGFVPFIDALVGRIAPGEESGIEAAVGTPVLLPGSVDQVRWAGGSAAVEGGAAFTAPR
                     PGLYWLVARGDTVGALAANADPRESDLAEASGRAVRSLWPTARVVTLDDVPAAAFGLA
                     ERTDLRGPLLWAALLLAVVELFVAGAGAPRRGSIE"
     gene            complement(15794..16717)
                     /locus_tag="VFU45_04905"
     CDS             complement(15794..16717)
                     /locus_tag="VFU45_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5358433.1"
                     /translation="MTGTSAAARPARYDLLDPLEAARLGGLELFAEGVVEGFLAGLHR
                     SPRRGFSVEFAEHRAYQPGDEPRYLDWRLLARTERPYVKQFEEETNLRAVLVVDVSRS
                     MAWSGAPGHTLTKLDYARRIAAALGLVLLRQRDATGCIAFDDDVRAVLPPRARSAQWH
                     RLCATLAALDPGAGTAAEPALRRVTDLLRRRGLVVFISDLLLDEALALQALRFLRHRG
                     HQVLVLHLMDPAEAELSGPAEARFEDPETHAAVVLRPRDWAPAYRAWVEHAVARWRRE
                     CGRHGIAYHRVTTDTPFGLALREVLVRPGLA"
     gene            16917..18323
                     /locus_tag="VFU45_04910"
     CDS             16917..18323
                     /locus_tag="VFU45_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEU5358434.1"
                     /translation="MLTLLGLAAALAAPAAPRPAAVRERPDLTAVPLYRLIDDARPKV
                     SIWTNSTNPYQRGDRVRVYLSAQTDAYVMVLRIDTDGRVRVLYPVEPWQDDFVRGGRT
                     FEILGRDDDNAFDVDDYPGVGYVFAVASDDPIHFDQFVRGDHWDYRTIADGRVRGDPY
                     VALTDFANQLVGQSDWDYDITSYDVEQHYDYPRFLCYDCHAYAAYSYWDPYTQYCPRF
                     RIVVYNDPYYYPYYYGGRRVSVVRPIRPQPRFVFKDYSGTGQYVTQHDRRPIRPTGDG
                     GQTFVPDRGRTGADLGGRGSIPAPQGFGRRPVRPDVSGGIGQPGQPPSREVQPDVGGI
                     GPMRRPVTGGQGQGNRQDPNAPPQPDQNRGRRPEVNPPAGGQAQPQPQPRQPQGNEVR
                     PERRPEVQPPQRVEPQRQPYRPPEEPRRVEPRNEAPPRIERPAPQPRVDRPPPERPRD
                     QPPRGGGGGQPELRRRKP"
     gene            18386..18751
                     /locus_tag="VFU45_04915"
     CDS             18386..18751
                     /locus_tag="VFU45_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5358435.1"
                     /translation="MTGSLADFDAFRSRMNERIFGADNLVVNRFFRLDGQAYEDGALG
                     RKTKEMLGLVSSMVLRCDDCITYHVIQCAGLGVTDEEFFEVFSVALVVGGSIVIPHLR
                     RAVDRLDEVRRESGRKAGR"
     gene            18851..20653
                     /gene="lepA"
                     /locus_tag="VFU45_04920"
     CDS             18851..20653
                     /gene="lepA"
                     /locus_tag="VFU45_04920"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU5358436.1"
                     /translation="MKQSRIRNFCIVAHIDHGKSTLADRLIEATGAVEKRVMRAQMLD
                     TMDLERERGITIKLNAVRMRYQALGGEEYELNLIDTPGHVDFTSEVSRSLAACEGAIL
                     VVDASQGLQAQTLSNLFLALDAGLEIIPVLNKIDLPGAEPERRAQEIMDLIGARREEI
                     LAVSAKEGTGIPALLEAIVARVPPPRGRPDSPFRALVFDSYFDRYRGAIPSIRVVDGV
                     IRPKMRIAFGSHPTDVYLVDEVGYLQLGQRPTDALEAGEVGYLVANLRTVGDTRAGDT
                     IFDADDQATELLPGYRDVKPMVFAGLYPTDASQYEALRDALEKLKLNDASLQYEPETS
                     TALGFGFRAGFLGLLHMEIVAERLQREFDLDLITTVPTVEYHVYRTNGEEVIVENPST
                     LPDPGGIDRIEEPYVKGRIMVPADYIGGIMKLGQDRRGVYLGMKYLDPTRVEFDWEFP
                     LGEIVLDFYDRLKSISRGYASFDYELSGYRPNDLVRLDMLINGEQIDAFSVIIHRDKA
                     YEWGRKVAEKLKELIPRQLFAVAIQAAIGSNVIARETISALRKDVLAKCYGGDVTRKR
                     KLLEKQKEGKKRMKQIGTVEIPQEAFLAVLQVED"
     gene            20738..21745
                     /gene="waaF"
                     /locus_tag="VFU45_04925"
     CDS             20738..21745
                     /gene="waaF"
                     /locus_tag="VFU45_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEU5358437.1"
                     /translation="MTGPPTLVIQTAFLGDVVLTIPLLDALAERFGPVDVVTTPGAAS
                     LLETHPAVRRVIRYDKKGADRGFRRFRRLVRSLRAERYARAYLPHQSWRSAALAVLAG
                     IPERIGFRESPARATYTERRARPRDLHESARLLHLLDPAPAPRPFRLALTEADRGRAA
                     AWLAAAGVAAPFVALAPGSIWGTKRWPHYAALVQKLDIAVVAVGGPEDAALGQAIVDA
                     APGRASSAVGALSLRESAALIERAAVLVTNDSAPLHLATAVGTPVVAIFGPTVPAFGF
                     GPLGADDAVVEHHAMPCRPCSAHGPQVCPLGHHRCMQELGPELVAREVHRILTLRGAR
                     R"
     gene            21742..>22461
                     /locus_tag="VFU45_04930"
     CDS             21742..>22461
                     /locus_tag="VFU45_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5358438.1"
                     /translation="MTHILGIDLGGTNIVVGCVAEDGSRVLGLVSEPTEAATGAEGVL
                     GRMLDLSRRSMALAAREQPKLDVIGVGIGAPGPLDAARNIIRLAPNLGWRDFPLRLRI
                     ADGLGIPAVLDNDANCAALGEWWQGAARRTTNAVCLTIGTGIGGGIIIDGKLYHGASS
                     AAGELGHTTIDVGGRRCKCGNYGCIEAYASGPAIAARAVEALEAGAASSLPGLVGGDV
                     GLITAQTVYDAAYAGDELALEV"
BASE COUNT         3163 a         8387 c         7874 g         3037 t
ORIGIN      
        1 cgtggtccag gatggcctgg aagagcggct taagatcggt gccgggatgc tcgagctccg
       61 tggtggccgt ccccgcccgg ctgctggtat aaaggaaagg agcgtcgagc tgctcggggc
      121 tcgcctccag gtcgatgaac aggccgagca cttcgtcgtg cacccggaag ggttcggcgt
      181 cctgccggtc gatcttgttg atggccacga tgggccggag gcccagggcc agcgccttcc
      241 gcgtcacgaa gcgggtctgc ggcatcggcc cttccgccgc gtcgaccagg atgaggacgc
      301 cgtccaccat ccgcaggatg cgctcgacct cgcccccgaa gtcggcgtgc ccgggggtgt
      361 ccacgatgtt gatcttggtc ccgccccagt gcaccgccgt gttcttggcc aggatcgtga
      421 tgccgcgctc acgctcgagc gggttggagt ccatcacccg ttcctcgacg tgctggttgg
      481 cccggaaggc ccccgcctgg cggagcatct ggtccaccag cgtggtcttg ccgtggtcga
      541 cgtgcgcgat gatggcgatg ttacgaatgt ccacagtaca tcccgtcgaa agcctgccgt
      601 ccccgggcca caggcgccac gaccgtgggc cttccggggg taccgggcgg catatataga
      661 cgcgtcaccg ggccgccgca agacagccgg aggcccgggg tcatccatgc ggaaccagac
      721 ctagtggtgg tggtgcccgg cgtgggaatg atgatggccg tgggcgtgcc cggcggccgg
      781 gccggccacc tccgcctcgg cgcaggggcc gccggtctcc agctggatgg tcacgtggcc
      841 gatgccgagc gccgccatgg cccgctggat cccggtgagc gcctcggggt gcgcccccag
      901 gtcggggacg acggcgtggc cgctcatcgc caccagcccg ctggtgacgg tccagacgtg
      961 gaggtcgtgc acggcggtga cgccggggag ggcgcgcatc cgcccctcga cctccgccag
     1021 cgagatgtgc gacggggtcc cctcgaggag gatatcgacg ctctcccgca ccaggcgcca
     1081 ggcgcccacc agcaccagga gcgcgatccc caccgacacg atcgggtcga ccagcgtcca
     1141 cccgctggtg aggatgatca ccccggccag gagcgcgccg atggagccga gggcgtcgcc
     1201 gaggacatga aggtaggcgg cgcggacgtt caggttctcg gtgtgcccgc cgtgcagcgt
     1261 gaagagcgag cccaggttgg ccgcgaggcc gaggagcgcc accccgaaga ggaccttccc
     1321 ctgcaccggt ggcggcgcgt gcagccggcc gaccgcctcg gcgatgatcc accccgcgat
     1381 gacgaagagc gccgcgccgt tgatgaacgc gccgaggatc tcccagcgga ggtacccgta
     1441 ggtgcgggtg gcgtcggcgg ggcgctcggc gatccacgcg gtgaagagcg agagcccgat
     1501 cgcggccacg tcggcgagca tatgtcccgc gtcggccagc agcgccagtg atccgctgat
     1561 caccgcgccc gccacttcgg cgccgcagat gaccaccgtc agcgccagca cccaccagag
     1621 ccgccgggtg tacgtccggg ggactcggat gaggcagagc gcgggggtgc tgggcgtcgg
     1681 ttgcatgggg gtgtatgcta taagctatca gcgatgcgcg atgcgcgatc cgctatccgc
     1741 tatccgttat ccgttatccg ctcgcgctga ccagcctctc cggtagtgat cgagcagtgt
     1801 tgaaactaat aggatcggtc gccgtccctc agctgttccc tgttccctgt ttcctgttcc
     1861 ctgttccctg ttcatcgttc gctcttcgcc ctcccgttgc gctgaccatc aggagccccg
     1921 cgtgtcccaa gcgcccgatc gccctgcgcc cctctctcgc cgcagcttca tcgttcaggc
     1981 cggcggggtg agcggcgccc tcctcgtcac ccccggcctc cggccggcgt ggctctcggc
     2041 actcgaggac tcgcgcacgg cggcagtcgc ccgcggcgcg gcgggccccg tcgatcccgc
     2101 gctcttcggc ggcctttcct ggcggatgct cggccccttt cgcggcggcc gcaccgacgc
     2161 ggtgagcggc gtccccggcc gccccgagga gttctacttc ggccacgtga acggcggggt
     2221 ctggaagacg aacgacggcg gccgcacctg gcgcccggtc ttcgacggac aacccgtcgc
     2281 ctccatcggc gccctcgcgg tggccccctc cgcccccgac gtgctgtacg tcgggagcgg
     2341 cgagtccacc ctgcgcgact cggtgggcta cggcaacggg atgtacaagt cgaccgacgc
     2401 cgggcgcacc tggacccaca tcggcctcga cgacacccag cacatcggca agatcgccgt
     2461 gcacccgaag gacccggacc tcgtcttcgt ggcggcgctg ggccattact acggcccgtc
     2521 gaaggagcgc ggggtcttcc gctcgaagga cggcgggaag agctgggaca aggtcctcta
     2581 caaggacgag aacgtcggcg ccatcgaggt cgcgatcgac ccgaatgacc cgcgcgtggt
     2641 ctacgccggg ctctggaata cccgccggcc gccctggttc acctatcccc cgaccaacgg
     2701 cccggggggc gggatcttca agtccaccga cggcggcgac acctggacgc agctgaccca
     2761 cggcctgcca tccgagggga tcgggcgcac cggcatcgcg gtggcgccga gcgactcgaa
     2821 gcgggtgtat gcagtggtgg actgcctggt ccccgagccg ggggcagcgg tgccgccgcc
     2881 cgacccgagc ggattcggtc gggacacgac gccgcgccag ggcgggttct tccgctccga
     2941 cgacggcggc gccacctgga tccggctctc cgccgaccag gcgctctggg gccgcggctg
     3001 gtacttcgag cacatcaccg tcgacccgac cgacgccgag gtggtgtacg tcgcaaacgt
     3061 ctccgtgtcg cgctcgaagg acggcgggca cacgtgggtg ccgctccgcg gctcgccggg
     3121 gggcgatgac taccatcagc cgtgggtctc ccccgacgac ccgaacacgc tcatcgtggc
     3181 gagcgatcag gggaccgtca tcacgcggaa cgccaaggcc gacgacccgc gccaggtgac
     3241 ctggacctcg tggctcaacc agcccaccgc gcagatctac cacgtctcgg tggactatcg
     3301 cttcccgtac tgggtcacgg gggcgcagca ggactccggc gcggtggcag tctgctcccg
     3361 aggcaagttc gccacgatct cgatgcgcga ctgggagccg atcggcgcag gaggggagag
     3421 cggcgccacc gccggcgacc cgctccaccc cggcatcatc ttcggcgagt cgggcacccg
     3481 gttcgacctg gcgctcaacc gcgagatccc gggcaccacc gcgcccgagg ccgccgacaa
     3541 gacgccgctc cggcgcgact ggacccagcc cctcgtcttc tcacccgccg atccgcacgc
     3601 gctctacttc gcggcgcagt tcctcttccg gtccaccgac ggcgcccgca cctggaccca
     3661 ggtgagcggc gacctgaccc gcaccgctcc cggtgtcccg cggaacctcg atgccgccgc
     3721 cgcggccgac gtccagcaga acgggcagcg cggcgtcatc tacaccgtgg caccgtcgcc
     3781 gctccaggcc cccctcctct ggatcggcac cgacgacggc gtcatccagg tgagccccga
     3841 cggcggcgac cactggcggg acgtgacccc gccggccctg agcgcgtgga gccgggtcac
     3901 caccgtcgag gcgagccact tcgaccccgc cgtggcgtac gcgagcgtcg accggcacca
     3961 gctcgaggac ttcgagccgt acatctaccg cacccgggac caggggaagc actggcagaa
     4021 gatcaccgac gggctgccgc ccggcgtcta cgtgcacgtg gtccgggagg acccggtccg
     4081 acgcgggctc ctgttcgccg gcaccgagcg cggggcgttc gtctcgttcg acgacggcga
     4141 ccactggcag cccttccagc tcgaccttcc ggtcacctcg gtgcgcgatt tccaggtcta
     4201 ccagaacgac ctgatcgtcg cgacgcacgg ccggggcttc tgggtcatcg acgacatctc
     4261 cccgctccgc caggtcggcg ccgaggtggc atccgccgac gcgtggctct tccagcccgc
     4321 cgacgccatc gacgtcatcg tggggaccga caacggcacg ccgctccaga aggacgagcc
     4381 gcaggcggag agccccccgg acggcgccta cctctattac tatctcaagg ccgacgcgcc
     4441 agcaccggtg acgctcgaga tccttgacgc ggcgggcgcc acgctccaca ccttctcgag
     4501 cgcggcgacg acggccccgc gcggcaacgg cgcgcccggc atccccaaca ccaccgcgct
     4561 ctggcgcgag ccggcggcgc ccttctccgc cgccgccggg ctccaccgcg cggtctggaa
     4621 gccgaacacc accccgcgcc cgggcttcgg cggacggcgc cggccccagg atccggtggt
     4681 ctccctgccg gggacgttca ccgcgcggct caccgtggcc gggaagagcc agacccggac
     4741 cttccacgtg cgccccgacc cccgcgccac ggcggaggac acgagcctgg gcacctagcc
     4801 cgcacgcggg cgccggcccg ctactatttg acccatgaac gacgctaccg gcggctccac
     4861 cctcttcggg ctcgtttcgg tcatcgcgct ggtcctggcc aacgcgttct tcgtggcggc
     4921 ggaattcgcg atggtcggga tccgccgctc ccggatcgag gagctggccc agggcggcgt
     4981 cggctcggcg gtgctggtcc gccgggcgct gacctccctc gaccggtaca tctccgccac
     5041 ccagctcggc atcacgctcg cctccctcgg actcggctgg gtgggcgagc cggcgctcgc
     5101 gcacctcttc gagcggtggt tcaccggcct ccccgccgcc ctccgcccct gggccaccca
     5161 cggcaccgcg gccacggtcg ccttcctcct catcaccggc ctccacatcg tcctcggcga
     5221 gctggtgccg aaggcgctcg cgatcgcgta ccccgagcgc gtcaccggcg tcaccatccg
     5281 gcccctctcc gccttcgtct gggcgatgac cgggcccatc gcggtgctca atggggcggc
     5341 ctggcagatc ctccggctcc tcggcctcag gcgccgctcc agccaggcgc cgctccattc
     5401 ccccgaggag atccgcatcc tcgtcgagca gagccagcag gcggggagcc tcggcaccga
     5461 ggacgcccgg ctcctcgaag gggtgttcga gttcagcgaa aagaacgccg aacaggtgat
     5521 gacgccgcgg acccagatga ccgccctcga ggcggggctc ccggtggagg aggccgccgc
     5581 catcgtggcc gagaccggac gctcgcgcta tccggtgtac gagggatcgc tcgacgacgt
     5641 ggtgggcgtg gtgcacgcca aggcgatcct ggccgcgctg cgccaggacc cgcaccagcc
     5701 ggtgcggaac gtgatgcggc cgcccctctt cgtgcccggg agccgggagg tggaggacgt
     5761 gctggccgac atgaagcggg tcaagtcgca cctggcgctg gtgctcgacg agtacggcgg
     5821 cacggcgggg ctcgtcacga tggaggacct gctcgaggag atcgtcgggc ccatcgagga
     5881 cgagtacgac cggcccgtcc ggacgcccgc gccgaccggc ggcgccctcg agctcgacgg
     5941 cgcgatgccc atcgccgagg ccaacgaacg gctcgacctg tcgctcgacg acgccgacta
     6001 caccacgctg ggcggctacc tcttcggcca gctcggccgc ctccccgccg tcggcgaccg
     6061 ggtgcgcgcg gaggggcggg aattcgagat cgcggagatg gagggacggc gggtcaagcg
     6121 ggtgcgggtg gtggggcgga gtgagaagta agaagtgaga agtaagaagt gagaagtgag
     6181 aagtaagaag tgagaagtaa gaagtgagaa gtgagaaggg agaagggaga agggggtagg
     6241 acggaacgag gatggcagag gaatcgtgaa cggacggggg tggcggggca actgaccggt
     6301 cacgcagcgg gccgcgtcac gctgcccccc accggcactt gaatgatgac ggtcgtcttc
     6361 acgcccggcg ccacgtttcc ccgatgcaac gccacaccag cctgcgcgcc tggcagaccg
     6421 ctcgatcggt cgcgttgggc gtattccggg ccaacgcgtc gcactggaga ccctgggtgg
     6481 cgccggcctt cgaacaagta ggtcgcgccg ccttgtcagt ccagttgaac atcgcggagg
     6541 gatactcgtt cggccggagt gcctcgttca cgaaccatct caggatcgcc tacggctccg
     6601 ccgtggagac agcggacctc ctcgagttcc tgcgagaact gggggcggtc acggccgccg
     6661 aatgcgaccg gctcatcgcg ctcaatcgcg aatgccaggc gtgcattgcg gggcttcttc
     6721 gcaaccgcaa cagctgggcc cggccggcat cgcaagccga cggacgggaa tagacgcctc
     6781 atcgccccgc tgcaccggcg cagacggcta cctccatcga ccaattttcc atcccacctc
     6841 tgccaccact tctcccttct cccttctcca ttctcccttc tcccttctcc cttctcactt
     6901 cttacttctc acttcttact tctttccgcc agctcgcatg ccagctccat cgccctccgc
     6961 atgctcgtcg cgtcggcctt cccctgcccc gcgatgtcga acgccgtgcc gtggtcgggg
     7021 gaggtgcggg ggaacgggag ccccagggtg atgttcacgc cttcgccgaa ggctgcgacc
     7081 ttgatggcgg tcatcccgac gtcgtgatac ggcgccagca ccgcgtcgaa ctccccccgc
     7141 atcgcgcgga cgaagacggt gtcggcgggg agcgggcccg cggcgccgag gatccgggcg
     7201 gcgggcgcca ggatgcgcgc ctcctcgtcg ccgaagagcc cctgttcccc ggcgtgcggg
     7261 ttgaccgcac agagcgccag ccgcggtgcc gcgatcccga accagtcgcg cagcgccgtg
     7321 agcgtcacct ggcccacgcg cacgatcttg tcggtggtga ggaggaccgg cacgtcgcgg
     7381 aaggggacgt gggtggtgac gagcaccacc cggagccggt cggcggcgag catcatggcc
     7441 accggcacgt cgcccgccag gtgggccagc cattcggtgt ggcccgggaa ggggaagccc
     7501 gccaggcgta tcgcgtgctt cgcgatcggc gcggtcacga tggcgtcgac ctcgccggcg
     7561 agcgccaggc gggcggcggc ctccaccgcg tggccggcga tcatcccggc gcgaacggcg
     7621 cgggggcggt cggcggcacc ttcgccgctg cccgcccccc agtggcccac cgacacccgt
     7681 cgcgccgccg gcagccccgc cagctggtcc tccgccccca ccagcgtcac cagggcgtcg
     7741 agcggcgttg cgagcgcgcg cgagacgatc tccggcccga tgccccgcgg gtcgccgagg
     7801 gtgacggcga tccgccgggt cacggcccgc ggacctcgac gtagttgtcg cgccggagcc
     7861 gggcgatgta gcgctggacc gcgagctgct tggcgagctg gggccggagc tggtccttca
     7921 cctcgtcgaa cgtcatcggc ccctcgcccc gcttcccggt gaccagtgcg aacgcccact
     7981 tggcgcggag cgagtcgacc gggttgggga gctcgaagat gtcggtgacc tgccccgagt
     8041 cggcctgcat gaccggatgg tacgccggcg ggagctggtc gagcggcacc aggtcgaagg
     8101 agcgctcctc ggaggagtcg gagtagatcc gctgcagcga gtcgaacgag gcccccgcca
     8161 tcacctggcc gcggagccgt tccgcgagct ggcgggccga atcggcctcg gcggcggtga
     8221 cggcgggggt gatgaggatg tggcgcgcct cgacctcggt cggctggacc cgctccacct
     8281 ggatcacgtg gaaaccgaag ggcgactcca ccggatcgga gatgacgccg ggcttgagcc
     8341 ggaaggccgc gtcgtcgaac tggcgcacca tctggccccg gcggaaccag ccgagctccc
     8401 cgccctggtc cttggtgccg gggtcctgtg aaaagcggcg cgccgcggtg gcgaagtcgg
     8461 cccccgcccg cagcgccttg gcgatcgagt cggcggtggc gtgcgccacc gcgcgcgcgg
     8521 cgggcgaggc gcggggcgcc accacgacct gcttgaacgc caccgccgcc ttccgcgtcc
     8581 ccttgtcggg ggtggcattg aagtagtcgc ggagctcacg ctcggtcggc gtcaccggct
     8641 tgagcttctg gtcgtcgaag agcgacgaga ccaggtggtt ctgcagcagg gtccggcgct
     8701 gctggtcggt gagccagcgc cggtactcct cgggggagat gaaccccgcc ttcttgagct
     8761 cgttcttgta gtcgagctcc gtggcgaaac tcgtgcggat ctgccggatg cgctggtcca
     8821 ccgcgtccga cacgtcctgg tcggtcacgt ggatcgtggt gtcgcgctcg gcctgctgca
     8881 ccaggagctc ctcgtcgacc aggtcgctca gcacctggcg cctgagcttc cggagcgagt
     8941 ccgggtcggt agggaccggc tgccccgcct gctggttcga gaagatccgc tcctcgacct
     9001 gcgagttgag gatcggcacg ttccccacca ccgccacgac gtggtccacc gactcgcccg
     9061 tgtcgccggg cgcctgggcc cggagcgcgg gcccgaggag cagtgtcgac gccagggcgg
     9121 cggcggcgaa cgcgcggccc atcaccgggg cgcgggcgcg ggcgtcgtgg ggagcgtcac
     9181 cggtgccgcg ttgttgtgct ccggcgacgg ggccggcgcc ttcccgtgcg cgcgggccgt
     9241 gctgtcctgc tccatctcgg ccttggcgcg gatgttgagc gcgagctcca ccgcgcgggc
     9301 cgtcccctgc tggtcgacgt ggctggggaa gcggtcacgc aggtagtcga ccatcggggc
     9361 cgggatcggg gtcatcccca cgcgtgcgcc gaagccgtag gccaggaact ggtcgacctt
     9421 gagcgcggcc gccttttccc ggtcggcgag cggcgtcgcc gggttcatga ggtcgtcgcc
     9481 caggcccatc ttggcccgca gcgtgtcgac gccggcgatg tacgcctgcc gcatcgtctc
     9541 catgtcggcc gccgacacct tgatgccgtg cgccttggcg tcctgcacga agagcacgtt
     9601 ctccgccacc cggctgatga agttcgagat ctgggagtcg gtggcctggc ccaggcccga
     9661 ggcgatctgc ggcggaagga cgttgaccca ccggaccatc tcgcggcctt cgagcttgcc
     9721 gccgtcatat gacgcgaacg cctcgctcga gtggcggtac gactccgggt cggtcaggag
     9781 ttcgcggagc ttgatcggcg ccttggacgc gaccttgagg tgcttggcct tggccaggct
     9841 gtcgaagtac atctggtcga gcaccatccc ggcgcgctga ttgaggaacg cctggaaccg
     9901 gtcgcggatc tcgttgaccg gcggccggcg cacgatgtgg aagccgtagg gcgtcgccac
     9961 gatgccgctc accgcccccg gcgccagcgt ccatgccgcg ctgtcgaacg acgtcacata
    10021 ggcgccgcgg ggggccggct gcatgtagcc ggagtccctg gcgctgccgg gatcgcccga
    10081 gaacttgagc gccagggcgc cgaagttggc gccggcgcgg atctgcgcgg cgagcccctc
    10141 gaccttcttc cgggccttgg ccttgaccga gtccggggcg ctcgccggca cggtgatcag
    10201 gatgtgctgc accaggcgca gggccatggt gtcggtgctg gcgtacgcgc tgtcgaccga
    10261 gtgcgccgtg aagttcgcgt ggcgcgccac cacggtgtcg tgccactgcc cggcgatccg
    10321 ttcggtcatc tccggccaca tcacctccgc caccgcggcg gaatcggtcg cgatcttccc
    10381 gtcggccacg gccagcgcga agaggtgata gttgatccag aggttggcca cgaagtcggc
    10441 ggtctcgggc gcgagctgcg cgcccttcag gcccgcgagg gtgaccgcca gcgactccgg
    10501 cgtcagggag atcggcccgg ccgatgccgc ttccttggca tgggcgctga agagcttgcc
    10561 gaccccgccc cgacagccgg caacgaggag gaggccgagc ccgaggcaca gcgtgttgcg
    10621 gcgcatctaa tctccctggg agtgtacggt cgcgtggacg gcgcggagcg cgcgcaccag
    10681 cgcggggacg agcggctcgc caccgagccg cgtcagacga agggcgagcg ggaccgtccg
    10741 gcggacctcc gcggccagct ggacgtcatc cagcgcgcgg gtgaggccgg tgaggcgggg
    10801 cgccgcgccg gcccggaagg agagccgggc ctcgtcgccc tggaccagca cgttctgcag
    10861 ccccagccgg gcgcccagca cccgcaggcg ggccacgtcg aggagcaccg ccgccggcgc
    10921 cgggaccggc ccgaaccggt cccgcagttc atcgtcgagc gcatcaattt cacccgatga
    10981 cacggcccgc gccagtcggc ggtagatgtc gagcttggcg tcgtcgtccg ggacgtagcg
    11041 gtccggcagg tggctcggct ggtcgatggc cacctccggc ggcaccggcg cctcggcccc
    11101 ccgggcctgt ccgtggagcg ccgtgacggt ctcctccagc caccgcaggt agaggtcgaa
    11161 ccccaccgcg tgcgcgtgcc ccgactgctc ggcgccgagc aggttccccg ccccgcggat
    11221 ctcgaggtcc ttcaccgcaa tccggtaccc cgccccgagc tccgtgtgat gctccagcac
    11281 cttgagccgc tcctgcgcca tcaggtcgag ctcgtccggc gccaggaggt agcagtacgc
    11341 ccgccggtgg ctccggccca cccggccgcg cagctggtag agctgcgcca ggccgaaccg
    11401 gtgcgcgtcg tgcaccacca tcgtgttggc gttggggacg tcgaggcccg attccacgat
    11461 catcgtcgac accaggatgt cgagctcccc ccgcacgaac gcctccatca ccccctcgag
    11521 ctcctccgcc ccctgctggc cgtgcgccac ccccacccgc gcccgcggcg ccagcgcccg
    11581 cacccgctgg gcgatcgtcg cgatggtctc gatccggttg tgcaccacga acacctgccc
    11641 gccccggtcg agctcccgcg cgaacgcctc ctcgaggagc gcgtcgtccc acggctccac
    11701 gaaggtcagc accggcgagc ggtcccgcgg cgcggtctcg atgagcgtca ggtcgcggag
    11761 tccggtgagc gagaggtgga gcgtccgcgg gatcggcgtc gccgtcatcg tcagcacgtc
    11821 caccgccagc ttgagcgcct tgatccgctc cttgtgcttg acgccgaagc gatgctcctc
    11881 gtccacgatc agcagcccca cgtcctggaa cgccacgtcg ggggagagga gccggtgcgt
    11941 cccgatcacg atgtcggtct cgccggtggt gagccgcgcg agcgccgcgg cctgctcccg
    12001 ggccgtgcgg aaccgcgaca gcacctccag ccgcaccggg aagtccgcca tccgctcctg
    12061 gaacgtgcgc ccgtgctgct ccgccaggat ggtggtcggc accagcacgg ccacctgctt
    12121 ccccccctgc accgccttga aggcggcccg caccgccacc tcggtcttgc cgtagcccac
    12181 gtcgcccacg aggagccggt ccatcggccg ggggagctcc atgtcgcgct tgacgtcctc
    12241 ggtggccttc cgctggtcgg gcgtatcctc atagaggaac cccgactcga gttcacgctg
    12301 catccgggtg tcgggcggga aggcaaagcc cgccgtcacc ttccgccgcg catagaggtc
    12361 caccagctcc ccggccatct gccggatggc ggcccgggtc cggtcccggg tcttctgcca
    12421 cgacgacccg ccgatggtat ggagcttggg cggcgggcgg tcgccgtcgt ccccggcggc
    12481 ccggtagtgc tccagcaggt cgagccggta gagcggcacg ttgacccggg tcccgccggc
    12541 gtactcgacc accgccacct cgagcgtccc cgcgccgatg gtgatggtct cgatcccgcg
    12601 atagatcccg atcccgtggt ccaggtggac cacgtagtcc cccgacgcca gcgccccggt
    12661 ggccgaggac ggcgccgcgg tgcgataccg ccgcgcccgc cggagccgcc gggcccgccg
    12721 gaagatctcg tggtcggtga gcacccggag ggtcgacatc acgaacccgc cgtccagggc
    12781 gcccaccttg agcgcggcgt tcacggtgcg ggccccctcg gagaggaggt cgtcgagccg
    12841 ctcccgctgc ccgtcattgt cgcacaggat gacgctcggg agctcgccgg ccaggatggc
    12901 gcggaggcgg ccgaggtcgc ggtcgacccg ctccggcggc aggaagccca tgtcgaggtc
    12961 gggcttggcg tcgcgcacgg tcaggcgcgc gaaccgcccc agcgagcgca tccaccggtc
    13021 gggcgactcc agcacctcct cgcgcgcggg cgcctcgtcg cccagccgcc gcgccaccgc
    13081 caggtggtgc tccgcgtcgc gccaggcccg acccacctcc tccgcgtcgg gtccctcccg
    13141 ctcctggatg atcagcgcat cggccggcag cagctcgagg agcgagcgcc gcacggccgg
    13201 cacctcggag cccgcgccct tcgggccggg cgcggagcgc gcgggcgccc gcaccggcag
    13261 gatggtcacg tcggccagcg ccccctgcga gcgctgggtg gtcaggtcga acgcgcggag
    13321 cccgatgagg tcgtcgcccc accactccgc ccgcaccggc tgtgccatcc cgaagccgta
    13381 cacgtcgacg atcccgccgc gcacgctgta ttccgccacc gccgacaccg tcggcacccg
    13441 ctggtagccc atcgactcca gccgctcggt caccgcgccg agcgccgggc cctccgccag
    13501 ccccttgccg tcctccggcg cgcggagggt gagccgcgcc gtctcgagcg ccgcggccat
    13561 cggtgtccgt tccgccgtcg cgcgcgcagt ggtcaccacg atccggatcc gcccgtgcgc
    13621 caggtcgtcg agggtctcgg cccgctcccc cgcgatctcg tagtgcgact cctcctcgcc
    13681 cagcgactcg cgctgggggt agaggcccac cgggagctcg gtgaggtgct tgaggtcggt
    13741 gagccagcgt tccgcctccg ccggtcccgg cgccaccacc gcgatgagcc gcgccggatg
    13801 cgcccgcgcg agcgaggcgg ccagcacggc ggggctggac cccggcagcc cgccgagcac
    13861 caggcgctca ccgcgcgccg gcaggcgggg gccgagctcg taggcgcccc gggtacggtc
    13921 gaaggcgtcg acgatcaggt cgaggggccc cgacttgggc gtggtgatgg cgggcggacg
    13981 ggcggtcacg gtcactcgat ggatcccctt cgcggcgctc cggcgccggc gacgaacagt
    14041 tcaacgacag cgagcagcaa cgcggcccag agcagcggcc cgcgcaggtc ggtacgttcg
    14101 gccagcccga atgcggcggc cggcacatcg tcgagcgtca ccacgcgggc ggtcggccag
    14161 agggaccgca ccgcgcgccc ggaggcctcg gcgaggtcgg attcccgcgg gtcggcgttg
    14221 gcggcgaggg cgccgacggt gtcgccccgc gccacgagcc agtagagccc cggccggggc
    14281 gcggtgaacg ccgccccgcc ctcgaccgcc gcgctgccac cggcccagcg gacctggtcc
    14341 accgatcccg gcagcagtac cggcgttccc acggcggcct cgatccccga ctcctcaccc
    14401 ggcgcgatcc ggccgaccag cgcgtcgatg aaggggacga accccgccac cagggggagc
    14461 tcggtccacg cgggctcgag ccggctgccg aggaggagca ccggccccga ccgcaccagc
    14521 cacggctccc cgccgacggt gatcagcacc ccggtccgcc cgctcccgtt cggcaccagc
    14581 cggtaccgcc gccagacccg ggccggccgg agccaggcgg agctgtcgat ggcggcagtg
    14641 tcgtcgagga gcgggccgta gcgccacgcc acgccgcgcg ccgcgagcgc ccggtcgagc
    14701 gcgccgacca gcgccgggtc cgccggcggg aagacgaccg acggcccggc acccaggctc
    14761 ccgacccgga gcccatcccc ggccaccgcc cggcggccgg ccaccagggt cgcgagcgcc
    14821 gccgcgacgt accggtcgct cgccgaccac gcgacccggg gggtcggcgc cacccgcacg
    14881 gccaccgtcc gagcatcgtc gccgcgaagc tcgtcgggat cgagtcgcgc ggtgagcgcc
    14941 gaccacccgg cccgtcctcc gggcaaggtg acgacggcgg ggcgccccgg ccccaccagc
    15001 gcctggcggg gtggcccggc gcccagcgtg atcgtgaccg ggaccggcgt cgagtcgtgg
    15061 tcgccggcga ccgtcaccgt caccgtgccg ccgtcaggac tccacggctc cgcgccgggg
    15121 aggaccgacg cgacgccgag attcggcggc ggcgcgcccg ccgccgacgg ggcgccgacc
    15181 aggacgggct ggcgggtggc ggcggcgctc agcgcgctcc cctgcaggtc ggacaggacc
    15241 acgacaccgc ccggcagccc cgcgccgtcg acgatccgcc ccgccacgtc gaccgcggca
    15301 ccgaggtcca tccgcatgcc gctcggctgg agcgagtcca cgacccggcg gaggaacaga
    15361 gggtcgcccc ggcgcggaat cccgtcggcg gtgaggagcc agagccggtc gcccgcaccg
    15421 gcgcgcgcca gcacgtccag ggccgcccgg cggagcccgg ccaggcgcgg cgttccaccg
    15481 acggtgacgc cggaggagag cgagttgtcg agcaccagca ccagcgccat cggcgcgtgg
    15541 ggccccgccg acccgagcgg cacggtgggg cccgcggccg ccagcaccag cgccaccacc
    15601 aggagcgtcc ggaggaggag caggagccac tgccgcagga gcaggcgccg ctcatgttgc
    15661 cgcgtcgcgt cccgcaggta gcgcaccgcc gggaacacga ccgtcggggg ctcgcgttgc
    15721 gcccggaagt ggaggagcag cgggatcgcc gcggcggcga gccccgcgag cgcccacggg
    15781 tagaggaatc cgatcacgcg agcccggggc gcaccagcac ctcccggagc gcgagcccga
    15841 acggcgtgtc ggtggtcacc cgatggtacg cgatcccgtg gcgcccgcac tcccgccgcc
    15901 accgcgccac tgcgtgctcc acccaggccc ggtaggcggg cgcccagtcg cgcgggcgga
    15961 gcaccaccgc agcgtgcgtc tccggatcct cgaaccgcgc ctccgccggg ccgctgagtt
    16021 ccgcctcggc cgggtccatc aggtggagca ccagcacctg gtggccgcgg tggcgcagga
    16081 agcgcagggc ttggagggcc agcgcctcgt cgaggaggag gtccgagatg aacaccacca
    16141 gcccgcgccg ccggaggagg tcggtcaccc gccggagcgc cggctccgcc gccgtcccgg
    16201 cgccggggtc gagcgcggcg agcgtggcgc agagccggtg ccactgtgcg gaccgcgccc
    16261 gcggcggaag cacggcccgc acgtcgtcgt cgaacgcgat gcagccggtg gcgtcacgct
    16321 gccgcaggag caccaggccg agcgccgctg cgatccgccg ggcgtaatcg agcttcgtga
    16381 gcgtgtgccc cggcgcgccg ctccacgcca tcgagcggct cacgtccacg acgagcacgg
    16441 cccggaggtt ggtctcttcc tcgaactgct tgacgtacgg ccgctcggtg cgggccagca
    16501 ggcgccagtc gaggtagcgg ggctcgtcgc ccggctggta ggcccggtgc tcggcgaact
    16561 ccaccgagaa tccccgccgg ggcgagcggt gcagcccggc caggaatccc tcgaccacac
    16621 cttcggcgaa caactcaagc ccgccgaggc gggcggcctc cagcgggtcg agcagatcat
    16681 accgggcggg acgtgccgcc gcggaggttc ccgtcattcc gggccgcaag ctacccggac
    16741 cgggcgccgc cgtcaacgcg acccggcgtg tcccgcctcg cggacatccc ggccggcacc
    16801 cacctcgggg ccaccccgca agcacctgca ccgcttggag ttgccgggac actggcgact
    16861 ggcacggctc ctgctcatcc ttcccttgcc gtccagttcc gtccctcgga ggacatatgc
    16921 tcaccctgct tggcctggcg gcggccctcg ccgcaccggc cgccccgcgc cccgccgccg
    16981 tccgggaacg ccccgacctg accgcggtgc cgctctaccg cctcatcgat gacgcgcgcc
    17041 cgaaggtgag catctggacc aactcgacca acccctacca gcgcggcgac cgggtgcgcg
    17101 tctacctctc ggcccagacc gacgcatacg tgatggtcct ccggatcgac accgacggcc
    17161 gggtgcgggt gctctatccg gtggaaccat ggcaggacga cttcgtccgg ggcggccgca
    17221 ccttcgagat cctcggccgc gacgacgaca acgcgttcga tgtcgacgac taccccggcg
    17281 tgggctacgt ctttgcggtg gcgtcggacg accccatcca cttcgaccag ttcgtccggg
    17341 gcgatcactg ggactatcgc accatcgccg acgggcgggt gcgcggcgat ccgtacgtgg
    17401 cgctcaccga cttcgccaac cagctcgtcg gccagagcga ctgggactac gacatcacgt
    17461 cctacgacgt cgagcagcac tacgactacc cgcggttcct ctgctacgac tgccacgcgt
    17521 atgcggccta ctcctactgg gacccctaca cccagtactg cccgcgcttc cggatcgtgg
    17581 tgtacaacga cccctactac tacccctact actatggcgg gcgccgggtg tcggtggtgc
    17641 gcccgatccg gccgcagccg cgcttcgtct tcaaggatta ttcgggcacc gggcaatacg
    17701 tgacccagca cgaccgtcgc cccatccggc cgacgggcga cggcggccag acgttcgtcc
    17761 ccgatcgggg gcgcaccggc gccgacctgg gcgggcgcgg cagcatcccc gcaccgcagg
    17821 ggttcgggcg ccggccggta cggcccgacg tgagcggggg gatcggccag ccgggccagc
    17881 cgcccagccg cgaggtccag cccgatgtcg gcgggatcgg cccgatgcgc cggccggtga
    17941 cgggcggcca gggccaaggg aaccggcagg atccgaatgc tcccccccaa cccgaccaga
    18001 accgcgggcg ccgccccgag gtgaacccgc cggccggcgg ccaggcgcag ccccagcctc
    18061 agccgcggca gccgcagggc aacgaggtcc gtccggagcg gcgccccgag gtgcagccgc
    18121 cacagcgggt ggagccgcaa cgccaaccgt accggccacc ggaggagccg cgccgcgtgg
    18181 aaccgcgcaa tgaggccccg ccccggatcg agcgccccgc cccgcaaccg cgcgtggatc
    18241 ggccgccccc ggagcgtccg cgggaccagc cgccccgcgg cggcggcggc ggccagccgg
    18301 agctgcggcg ccgcaagccc tgacggcagc gccacgaagg tcgacggggc ccggatcacg
    18361 ggccccgtcg ccttatattt gccccatgac cggatccctc gccgatttcg acgccttccg
    18421 cagccggatg aacgaacgga tcttcggcgc cgacaacctg gtcgtcaacc gtttcttccg
    18481 cctcgacggc caggcctacg aggacggcgc gctgggcagg aagaccaagg agatgctggg
    18541 cctcgtctcc tcgatggtcc tccggtgcga cgactgcatc acctatcacg tgatccaatg
    18601 cgccgggctc ggtgtcaccg acgaggagtt cttcgaggtg ttctcggtgg cgctggtggt
    18661 gggcggcagc atcgtgatcc cgcacctgcg gcgcgcggtg gaccggctgg atgaggtgcg
    18721 gagggagagc gggcgaaaag cgggtcggta gcgggcgaag ggcgggcacc cgctgtcacc
    18781 cgcagcgccc gcggtttgtc cgcggttcgc ccgctattag cccgctcccc ccccgcttat
    18841 cttccgcgcc atgaagcagt cccggatccg caatttctgc atcgtggcgc acatcgacca
    18901 cggcaagtcg accctcgccg accgtctcat cgaggccacg ggggcggtcg agaagcgcgt
    18961 gatgcgggcg cagatgctcg acacgatgga cctcgagcgc gagcgcggca tcaccatcaa
    19021 gctcaacgcc gtgcggatgc gctaccaggc gctgggcggc gaggagtacg agctcaacct
    19081 catcgacacc cccgggcacg tcgacttcac ctctgaggtg agccgctcgc tcgccgcgtg
    19141 cgagggcgcc atcctggtgg tcgacgcgtc gcaggggctc caggcgcaga cgctctccaa
    19201 cctgttcctc gcgctcgacg cggggctcga gatcatcccg gtcctcaaca agatcgacct
    19261 ccccggtgcc gagccggagc gccgcgccca ggagatcatg gacctcatcg gcgcccgccg
    19321 ggaggagatc ctggcggtga gcgccaagga gggcaccggg atccccgccc tcctcgaggc
    19381 gatcgtcgcg cgggtaccgc caccccgcgg ccgccccgac tccccctttc gcgcgctcgt
    19441 cttcgacagc tacttcgacc gctatcgcgg cgccatcccc agcatccgcg tggtggacgg
    19501 cgtcatccgg ccgaagatgc ggatcgcgtt cgggtcgcat ccgaccgacg tgtacctggt
    19561 cgacgaggtg ggctacctcc agctcggcca gcgccccacc gacgcgctcg aggcgggcga
    19621 ggtgggctac ctcgtggcca acctccgcac cgtgggcgac acccgcgccg gtgacacgat
    19681 cttcgacgcc gacgaccagg ccaccgagct cctgccggga taccgcgacg tcaagccgat
    19741 ggtgttcgcc gggctctacc cgaccgacgc cagccagtac gaggcgctgc gcgatgcgct
    19801 cgagaagctc aagctgaacg acgcctcgct ccagtacgag cccgagacct cgaccgcgct
    19861 cggcttcggg ttccgcgccg gcttcctcgg cctcctccac atggagatcg tggcggagcg
    19921 gctgcagcgg gagttcgacc tcgacctcat caccaccgtg ccgacggtcg agtaccacgt
    19981 gtaccgcacc aacggcgagg aggtcatcgt cgagaacccc tccaccctcc ccgaccccgg
    20041 cggcatcgac cggatcgagg agccgtacgt gaaggggcgc atcatggtcc ccgccgacta
    20101 catcggcggg atcatgaagc tgggccagga ccgccggggc gtgtacctcg ggatgaagta
    20161 cctcgacccg acccgggtgg agttcgactg ggagttcccc ctcggcgaga tcgtgctcga
    20221 cttctacgac cgcctgaaga gcatcagccg cggctacgcg tcgttcgact acgagctgtc
    20281 gggctaccgc cccaacgacc tggtgcggct cgacatgctc atcaacggcg agcagatcga
    20341 cgcgttcagc gtgatcatcc accgggacaa ggcctatgaa tggggccgca aggtggcgga
    20401 aaagctgaag gagctcatcc cccgccagct ctttgccgtg gccatccagg ccgcgatcgg
    20461 ctcgaacgtc atcgcccgcg agacgatcag cgcgctccgc aaggacgtgc tcgccaagtg
    20521 ctacggcggc gacgtgaccc ggaagcggaa gctcctggag aaacagaagg aagggaagaa
    20581 gcggatgaag cagatcggca ccgtggagat cccgcaggag gcgttcctgg cggtgctgca
    20641 ggtggaagat tagggaacgg cccacgggcg ctcagacgct cggatgctcg agcgcccgga
    20701 cgtccgcacg cctgtgcctc tctgcgtcgg agcgtccgtg accggtcccc ccaccctcgt
    20761 catccagacc gccttcctgg gcgacgtcgt gctgacgatc ccgctccttg acgcgctggc
    20821 ggagcggttc ggcccggtgg acgtcgtcac gacgccgggc gcggcctcgc tcctcgagac
    20881 ccaccccgcg gtgcggcggg tcatccgcta cgacaagaag ggagcggacc ggggcttccg
    20941 gcgcttccgc cggctggtcc ggtcgctgcg ggcggagcgg tacgcccgcg cgtacctgcc
    21001 gcaccagtcc tggcggtcgg cggcgctggc ggtgctggcg gggatccccg agcgcatcgg
    21061 cttccgcgag agccccgcgc gcgcgaccta caccgagcgg cgcgcgcggc cccgcgacct
    21121 ccacgagtcg gcgcggctcc tccatctcct cgatccggcg ccggcgccgc gcccgttccg
    21181 cctggcgctc accgaggccg accgcgggcg cgccgcggcc tggctggcgg cggccggcgt
    21241 cgcggcgccg ttcgtggcgc tcgcccccgg ctcgatctgg ggcacgaagc gctggcccca
    21301 ctatgcagcg ctggtgcaaa agctcgatat tgcggtggtg gcggtggggg gcccggagga
    21361 cgcggccctg ggtcaggcga tcgtcgacgc ggcgccgggc cgggcgtcga gcgcggtggg
    21421 cgctctctcg ctccgtgagt cggcggccct catcgagcgc gcggcggtgc tcgtgaccaa
    21481 cgactccgcc ccgctccacc tggcgacggc cgtcgggacg ccggtcgtgg cgatcttcgg
    21541 gcccacggtc cccgcgttcg ggttcggccc cctcggcgcc gacgacgcgg tggtcgagca
    21601 ccacgcgatg ccctgccgcc cctgctcggc ccacgggccc caggtctgcc cgctcggcca
    21661 ccatcgctgc atgcaggagc tcggccccga actcgtggcg cgcgaagtcc atcggatcct
    21721 caccctgcgc ggagcccgcc ggtgacgcac atcctcggca tcgacctggg cggcaccaac
    21781 atcgtcgtcg gctgcgtggc cgaggacggg tcgcgcgtgc tggggctcgt gagcgagccc
    21841 accgaggccg cgaccggcgc cgagggggtg ctcggccgga tgctcgacct gtcacgccgg
    21901 tcgatggcgc tcgcggcccg ggagcagccg aagctcgacg tcatcggcgt cgggatcggc
    21961 gccccggggc ccctcgacgc cgcgcggaac atcatccgcc tggcccccaa cctcggctgg
    22021 cgcgatttcc cgctccggct ccggatcgcc gacgggctcg ggatccccgc cgtgctcgac
    22081 aacgacgcca actgcgcggc gctgggcgag tggtggcagg gcgccgcgcg ccggaccacc
    22141 aacgccgtgt gcctcacgat cggcaccggg atcggcggcg ggatcatcat cgacggcaag
    22201 ctctaccacg gcgcctcgag cgccgccggg gagctggggc acaccaccat cgacgtcggc
    22261 ggccggcgct gcaagtgcgg caactacggg tgcatcgagg cctacgcgtc ggggcccgcc
    22321 atcgccgcgc gcgccgtgga ggcgctcgag gccggcgccg cgtcgtcgct gccggggctg
    22381 gtgggggggg atgtcggact gatcaccgcc cagaccgtgt atgacgccgc ctacgccggc
    22441 gacgagctgg cgctcgaggt g
//
LOCUS       DASUAP010000196         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_578278, whole genome shotgun sequence.
ACCESSION   DASUAP010000196 DASUAP010000000
VERSION     DASUAP010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578278"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1324
                     /locus_tag="VFU45_04935"
     CDS             302..1324
                     /locus_tag="VFU45_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358439.1"
                     /translation="MPTIARIAGFVAAFALAAAPVAAQSYHVTHTYTLGGDGGWDYLA
                     LDTVGNRMFITRGDRAMVVDPASGKVLGEVPGIVGAHGVAFSHASGLGFATSGRDSSV
                     IVFDLKTLKVVKRTPAAPDADAILFDPATKRVFTFNGDSHNSTVIDATTGDRVGTVDL
                     GAGPEFGVSAGDGHLYVNLETTSEVAEIDAHSMKVTRKWSIAPCESPSGLAIDVAHHL
                     LFSVCRNGTMAVSDAVKGVVVAMAPIGRGVDAARFDPATGFAFASNGEGTLTVVHEDS
                     PTAFHVVETDTTWPGARTMELDPRDHRIYTVSAKFGPMPAAAPGERRRRPPMVPGTFT
                     LLVLER"
     gene            1375..1445
                     /locus_tag="VFU45_04940"
     tRNA            1375..1445
                     /locus_tag="VFU45_04940"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1407..1409,aa:Cys,seq:gca)
     gene            complement(1528..1986)
                     /locus_tag="VFU45_04945"
     CDS             complement(1528..1986)
                     /locus_tag="VFU45_04945"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5358440.1"
                     /translation="MTGLPRETQPDPALEARVVATLVAAGLVRRRRMWPVWVAAAAAI
                     VLTVGVTMLRPKHMRAHDQTYVVLLYEDSTYRPAPAGQDAERVAVLARWGDSLVAAGK
                     FERAGRLRGPGPLGGLFIVRATDDADAARIAASCPFTQWGGHIEVKRFDE"
     gene            complement(1983..2489)
                     /locus_tag="VFU45_04950"
     CDS             complement(1983..2489)
                     /locus_tag="VFU45_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5358441.1"
                     /translation="MTDDLELVDRFLHGRGEEAFRALYRAHTPALYALALRLTGGDQG
                     EAEDLVQESWVRALTALRTFRAESALRTWLCGVLVNVRRGRLRAEWRIVDAPEVEPIA
                     KGNGPDDAIDLERAIATLPEGARDVYVLHDVHGYTHREIAELLGIVEGTSKSQLNRAR
                     SLLRSALP"
     gene            2785..3492
                     /locus_tag="VFU45_04955"
     CDS             2785..3492
                     /locus_tag="VFU45_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358442.1"
                     /translation="MSRTAGKVVGWGSLALAILSGAAAPAQAQGTQAPASPSPYPTMA
                     PIAQYHMDRDAEIALARTAAPASISRDAEIMVLGRKNYEVAVRGRNGFVCMVGRAFAG
                     PLNNPEFWSPRNRSPICLNPPAARSLWPYAVKQTGLALAGATKAQITDAIKAAVARKE
                     LGAPELGSMAYMMSKQAYLTDQGSHNLAHVMFELPRSEAFQSDSAFSVSWDPAPVIEF
                     NVPVGHWSDGTDASGKS"
BASE COUNT          540 a         1241 c         1238 g          541 t
ORIGIN      
        1 cgtcgaggac gacgaggtcg tactcgttga ccggcaggag ggcgagcgcc tgggcgccgg
       61 tcgacgcgac gtccggcgcc caggtggcct cccggacggt gcgcgccagg aggtcggcca
      121 cgtccggatc atcttcgacg acgagcacgc gcaacgcccc tccttggccg gtaccggcat
      181 caacgttaac cgctgccccc tgatcgcgcc gcgctgtcat cccgctgtca tccggcggcg
      241 tgacattgcc ggcctctcct cgccagccac cggacaccga tccaagggaa gggccgccgc
      301 aatgccaacc atcgcccgca tcgccggatt cgtcgccgcg ttcgcgctcg cagcggcgcc
      361 ggtcgccgcg cagtcgtacc acgtgacgca cacctatacg ctgggcggcg acggagggtg
      421 ggactacctc gcgctcgaca ccgtcgggaa ccggatgttc atcacccggg gcgaccgcgc
      481 gatggtcgtc gatccggcgt cgggcaaggt gcttggtgaa gtcccgggga tcgtcggggc
      541 gcacggggtc gccttctccc atgcatccgg tctcgggttc gccacctcgg ggcgggacag
      601 ctcggtcatc gtcttcgacc tcaagaccct caaggtggtc aagcggaccc cggccgcacc
      661 cgatgccgac gcgatcctct tcgaccccgc gacgaagcgc gtcttcacgt tcaatggcga
      721 ctcgcacaat tccaccgtca tcgacgccac caccggcgac cgggtcggca cggtggacct
      781 gggtgcgggg ccggagttcg gcgtctccgc cggcgacggt cacctgtacg tcaacctgga
      841 gacgacgtcg gaggtcgccg agatcgacgc gcactcgatg aaggtgacgc ggaagtggtc
      901 gatcgccccg tgtgagtcgc cgagcggcct cgcgatcgat gtggcgcacc acctcctctt
      961 cagcgtctgc cgcaacggga cgatggcggt gtcggatgcg gtgaagggcg tggtggtggc
     1021 gatggcgccg atcgggcgcg gggtggatgc cgcccgcttc gacccggcga ccgggttcgc
     1081 gttcgcctcc aatggcgagg ggacgctcac ggtggtccat gaggattcgc cgacggcatt
     1141 ccacgtggtc gagaccgata ccacctggcc cggcgcccgg acgatggagc tcgacccccg
     1201 ggaccatcgg atctacaccg tcagcgccaa gttcggcccg atgcctgccg ccgccccggg
     1261 cgagcgccgc cggcgcccgc cgatggtgcc cgggacgttc acgctgctgg tgctggagcg
     1321 gtagggtggt cggctttccg gcgcccggcg gggggtgtag tttttacccc cgctggcgcc
     1381 gtagccaagt ggtaaggcag aggtctgcaa aacctccatc accggttcga ctccggtcgg
     1441 cgcctcttgg gcgatgatga ccaaggccac ctcacacggg gtggccttgg tcttggtcgt
     1501 gcccggcggc ggccggggat ggcggcgtca ttcgtcgaat cgcttcacct cgatgtgccc
     1561 tccccactgg gtgaacgggc atgaggccgc gatccgggcc gcatcggcgt cgtccgtggc
     1621 acgcacgatg aacagcccac cgagcggtcc gggccctcgg aggcggccgg cgcgttcgaa
     1681 cttcccggcc gcgacgaggc tgtcccccca ccgtgcgagc acggccacgc gctcagcatc
     1741 ctgccccgcg ggcgcaggcc ggtatgtgga gtcctcgtag agcagtacga cataggtctg
     1801 gtcgtgcgcc cgcatgtgct tcgggcggag catcgtgacg ccgaccgtca gcacgatcgc
     1861 cgccgccgcc gccacccaca ccggccacat acgccgccgc cggacgagcc ccgccgcgac
     1921 cagcgtggcc acgacgcggg cctccagcgc cgggtccggc tgcgtctccc gcggaaggcc
     1981 ggtcatggca gtgcgctccg aagcagggac cgggcacgat tgagctggct cttcgacgtg
     2041 ccctcgacga tgccgagcag ctcggcgatc tcccggtggg tgtacccgtg cacgtcgtgg
     2101 aggacgtaca cgtcccgcgc gccctcgggg agcgtggcga tcgcgcgctc gaggtcgatc
     2161 gcgtcatcgg gcccgttgcc cttcgcgatc ggctcgacct ccggtgcatc cacgatgcgc
     2221 cactcggcgc gaaggcgtcc gcgccggacg ttcacaagca ctccgcacag ccacgtgcgc
     2281 agggcggact ccgctcgaaa ggtccgcagg gcggtcagcg cccggaccca gctctcctgc
     2341 accaggtcct ccgcttcgcc ctgatccccg cccgtgaggc gcagcgccag ggcgtacagc
     2401 gccggcgtat gcgcgcgata cagtgcgcga aaggcctctt cgccgcgccc gtgaagaaac
     2461 cggtcgacga gttccaggtc gtccgtcatt gagcccggca ggtgttcatc cgatcgcgcg
     2521 agacctcgat gaggtgggtc cggatcacgt ccccgatggc ggcaggggcc gatatgagcg
     2581 aggggtgcgc ccgggcacca acccccgtcc ttcgtggaag gtactgatgt gtagcgcccg
     2641 atggccaaag ggttggaatg cgcgccgcct ggtcggcgtc aggcctgttc caaccttggc
     2701 cccgggtgga ggcacccatc tccgatggcg cccatcgcgg cgacggcgcg cgctgagcga
     2761 gacacctcga acaggagacg aacgatgtcc cgaactgctg gcaaggtggt cggttgggga
     2821 agcctggcgc tggccatcct gtccggtgcg gcggcaccgg cccaggcgca ggggacgcag
     2881 gcgccggcaa gcccgtcgcc gtaccccacc atggcgccga tcgctcaata ccacatggat
     2941 cgcgacgccg agatcgcgct ggcgcgcacc gcggccccgg cgtcgatatc ccgcgatgcc
     3001 gagatcatgg tgctgggacg gaagaactac gaagtcgcgg tccggggcag gaacggcttc
     3061 gtctgcatgg tggggagggc gttcgcgggg ccgctgaaca atccggaatt ctggagtccc
     3121 aggaaccgca gtcccatctg cctcaaccct ccggcggcgc ggtccctctg gccgtacgcc
     3181 gtgaagcaga ccgggctggc attggccggt gccacgaagg cgcagatcac cgatgcgatc
     3241 aaggcggccg ttgccaggaa ggagctgggt gcgccggagc taggctccat ggcctacatg
     3301 atgtccaagc aggcgtatct gacggaccaa ggcagccaca acctggcaca cgtgatgttc
     3361 gagcttccgc gcagcgaggc gtttcagagc gactctgcct tctccgtgtc ctgggacccg
     3421 gcaccggtga tcgagttcaa cgttccggtg gggcattggt cggacggcac cgacgcgtcc
     3481 ggtaagagct aggctaccca agcccggagc gcacgcggcg ggtcagtggt cccgccagcc
     3541 ggtctggatc gtacacgggt
//
LOCUS       DASUAP010000197         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_687015, whole genome shotgun sequence.
ACCESSION   DASUAP010000197 DASUAP010000000
VERSION     DASUAP010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687015"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VFU45_04960"
     CDS             complement(<1..1338)
                     /locus_tag="VFU45_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5358443.1"
                     /translation="MSGNAPGEPGTFPFTRGIHPGMYTTRLWTMRQYAGFGTAEETNA
                     RFRQLLAAGQSGLSTAFDLPTQMGYDSDHPMAAGEVGRVGVAIDTVDDLAVLFGGIAL
                     DQVTTSMTINATAAILLAMYVVVGEEQGVPRTRLGGTVQNDVLKEYIARGTYIYPPEP
                     SLRLTTDIFRFVVAEKMNFNPISISGYHIREAGATAAQEVAFTLADGLEYVRHGIAAG
                     VPLSGFGPRLSFFFAAHNDLFEEVAKFRAARRLWATLVRERFQADDATARLRFHTQTG
                     GVTLQARQPLNNIARVTLQALAAVLGGTQSLHTNGYDEALALPSAEAATLALRTQQVI
                     AFESGVVNAVDPLGGSGYVEALTDRVERDARALIAEIDELGGAAAAIEKGFFQKAIAE
                     SAWVQQKRQESGEQVVVGVNRFEDDSPTPVLHQPDFTALAARQVARLREGKKKR"
     gene            complement(1335..2348)
                     /gene="fbp"
                     /locus_tag="VFU45_04965"
     CDS             complement(1335..2348)
                     /gene="fbp"
                     /locus_tag="VFU45_04965"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEU5358444.1"
                     /translation="MQSRTSVTTIERFIVDQEQRFPDATGELSNLLYDVALAAKIISA
                     NIRRAGLVNILGSTGVTNVQGEEQQRLDAFANETLQHALNHTGRVCVMASEEDDGPIP
                     VPVEFNTGKYAVLFDPLDGSSNIDVNAAVGTIFSVYRRVTTAGGGALEDLLQPGRKIV
                     AAGYVMYGSSTMLIYSTGIGVHGFTLDPSIGEFLLSRDQVRMPRSGKYYSVNESNFAR
                     WDRRTQKAVRGFHGDFPDRIAGKNSRYIGSLVADFHRNLMAGGIFMYPADTKNPRGKL
                     RLLYEACPMAFIAEQAGGAATDGSFPILDVVPTDLHQRTPLVIGCRDDVDWVRQVMAE
                     PMP"
     gene            complement(2377..>2762)
                     /locus_tag="VFU45_04970"
     CDS             complement(2377..>2762)
                     /locus_tag="VFU45_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358445.1"
                     /translation="AVLLLRWGRFRALLAGDAGIAAEARMAGRAGHVHLLKVGHHGSR
                     GASGPAWLHELAPEVAVVSVGAGNRYGHPTAEALARLTAAGASVWRTDQDGGVAVAVD
                     SVTMEVRGGGRRERYALPAPAPEQR"
BASE COUNT          377 a          957 c         1006 g          422 t
ORIGIN      
        1 ccgcttcttc ttcccctccc gcagccgggc gacctgccgg gccgcgaggg ccgtgaagtc
       61 gggctggtgg agcaccggcg tcggggaatc gtcctcgaag cggttgaccc ccaccaccac
      121 ctgctccccg ctctcctggc gcttctgctg gacccaggcg ctctcggcga tggccttctg
      181 gaagaatccc ttctcgatgg cggcggcggc gcctcccagt tcgtcgatct ccgcgatcag
      241 cgcccgcgcg tcgcgctcga ctcggtcggt cagcgcctcg acgtacccgc tcccgccgag
      301 cggatcgacg gcgttcacga cgccgctctc gaaggcgatg acctgctggg tgcggagcgc
      361 cagggtggcg gcctcggcgg acgggagcgc cagggcctca tcgtacccgt tggtgtggag
      421 cgactgggtc ccgcccagga ccgcggcgag cgcctgcagc gtgacgcggg cgatgttgtt
      481 gagcggttgg cgcgcctgca gcgtgacgcc gccggtctgg gtgtggaacc ggagtcgggc
      541 ggtggcgtcg tcggcctgga agcgctcccg gacgagcgtg gcccagaggc gccgcgccgc
      601 gcggaacttg gcgacctcct cgaagaggtc gttgtgcgcg gcaaagaaga agctgaggcg
      661 gggcccgaac ccggagagcg gcaccccggc ggcgatgccg tgccgcacgt actcgagccc
      721 gtcggcgagg gtgaacgcca cttcctgggc ggcggtggcg cccgcctcgc ggatgtgata
      781 gcccgagatc gagatggggt tgaagttcat cttctcggca acgacgaacc ggaagatgtc
      841 ggtggtgagc cggagcgagg gctccggggg atagatatac gtgccgcgcg cgatgtactc
      901 cttgagcacg tcgttctgca ccgtgccgcc gagcctggtg cgcgggacgc cctgctcctc
      961 gcccaccacg acgtacatcg cgagcaggat ggcggcggtg gcgttgatcg tcatcgacgt
     1021 ggtgacctgg tcgagcgcga tgccgccgaa gagcaccgcc aggtcatcca ccgtgtcgat
     1081 ggccaccccg acccgcccca cctcgccggc cgccatgggg tggtcggagt cgtagcccat
     1141 ctgcgtcggc aggtcgaacg cggtcgagag cccggactgg cccgccgcga gcagctgccg
     1201 gaagcgcgcg ttggtctcct ccgcggtgcc gaacccggca tactggcgca tggtccagag
     1261 ccgggtggtg tacatccccg ggtggatgcc gcgggtgaag gggaatgtcc ccggctcgcc
     1321 gggcgcgttc ccgctcacgg catcggctcc gccatcacct gccggaccca gtcgacgtcg
     1381 tcccggcacc cgatcacgag gggggtgcgc tggtgcaggt cggtcggcac cacgtcgagg
     1441 atcgggaagc tgccgtcggt ggccgccccg cccgcctgct ccgcgatgaa cgccatcggg
     1501 cacgcctcat agaggagccg gagcttgcca cgcgggttct tggtgtcggc gggatacatg
     1561 aagatcccgc cggccatcag gttgcggtgg aagtccgcca ccagggaccc gatgtagcgg
     1621 ctgttcttcc cggcgatgcg gtcggggaag tcgccgtgga agccgcgcac cgccttctgg
     1681 gtgcggcgat cccagcgggc aaagttgctc tcgttgacgc tgtagtactt gccgctgcgg
     1741 ggcatgcgga cctgatcgcg cgagagcagg aactcgccga tggaggggtc gagcgtgaag
     1801 ccgtgcaccc cgatgccggt ggagtagatg agcattgtgc tggagccgta catcacgtac
     1861 ccggcggcca cgatcttccg gcccggctgc agcaggtcct cgagcgcccc gcccccggcg
     1921 gtggtgaccc ggcggtagac gctgaagatc gtcccgaccg cggcgttcac gtcaatgttg
     1981 gaggagccgt cgagcgggtc gaagagtacg gcgtacttcc cggtgttgaa ctccaccggc
     2041 accgggatgg gcccgtcatc ctcctccgac gccatcacgc acacccgacc ggtatggttg
     2101 agcgcgtgct gcagcgtctc gttggcgaag gcgtcgagcc gctgctgctc ctcgccctgg
     2161 acgttggtga cgccggtcga gccgaggatg ttgaccaggc ccgcgcgccg gatgttggcc
     2221 gagatgatct tggcggcgag ggccacgtcg tagagcaggt tggacagctc cccggtcgcg
     2281 tccggaaagc gctgttcctg gtcgacgatg aaccgctcga tggtggtgac cgaggtgcgg
     2341 ctctgcacgc gggctccggg tgtgaacggt gaacggtcaa cgctgctcgg gggccggcgc
     2401 gggcagggcg taccgctccc ggcgcccccc gccccgcact tccatcgtca cgctgtcgac
     2461 ggccacggcc accccgccgt cctggtcggt gcgccacacc gatgcccccg ccgcggtgag
     2521 ccgcgcgagc gcctcggcgg tcgggtgccc gtaccggttg ccggcgccga cgctcaccac
     2581 cgccacttcg ggggcgagct cgtgcagcca ggccggcccc gacgcgcccc ggctgccgtg
     2641 gtgcccgacc ttgagcaggt gcacgtggcc ggcgcgtccc gccatgcgcg cctcggccgc
     2701 gatccccgca tcgcccgcca ggagggcgcg gaaccggccc caccggagca ggagcacggc
     2761 gg
//
LOCUS       DASUAP010000198         6266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1272793, whole genome shotgun sequence.
ACCESSION   DASUAP010000198 DASUAP010000000
VERSION     DASUAP010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272793"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="VFU45_04975"
     CDS             complement(<1..1350)
                     /locus_tag="VFU45_04975"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HEU5358446.1"
                     /translation="MTDSAAPARSRPTAPDLHHPSLYINRELSWLEFNRRVLHQALDA
                     RTPLLERLKFLAIYASNLDEFFQVRVSGLKQQLAAGVVERPADGMTPEDQLTVIGRTV
                     REMQARADACLVEELFPALAARGVELMDMTAVAPEEREALDRWYEANVFPVLTPLAVD
                     PAHPFPHISNLSLSLAVMLRGDQGEERFARVKVPRLLPRWVPLGAGHRFVPLEQVIGA
                     NLEALFPGVEIVGWSTFRVTRNTDVQIDPEEAEDLLSLIQEELRNRRFGEVTRVEVSP
                     RTPVAIRQMLLDELAEEAPGWLGGEDIYEVRGIMAAGDLLGLAGIEVPELRDPAFHPG
                     TPPRFAAARTVFEALREGDVLAHHPFDSFATTVDRFIREATEDPDVVAIKLTLYRTGA
                     DAGIARALQHAAERGKQVAVLIELQARFDEENNITWARRLEDAGVHVSYGVAGLKTHT
                     "
     gene            complement(1391..1795)
                     /locus_tag="VFU45_04980"
     CDS             complement(1391..1795)
                     /locus_tag="VFU45_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU5358447.1"
                     /translation="MRELALRWAVTAAALFLAIAIVPGLHFDTSDGGVLRLFLVAAVF
                     GIVNAVLKPLLSILTCPLILLTFGLFTLVVNAILLEATGWLSTRWGLGFSVAGFWPAF
                     WGGLVVGISSALLLAATRPRRRPPEVEIHTLD"
     gene            complement(1795..2880)
                     /gene="mnmA"
                     /locus_tag="VFU45_04985"
     CDS             complement(1795..2880)
                     /gene="mnmA"
                     /locus_tag="VFU45_04985"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU5358448.1"
                     /translation="MPEALAGPAGRPRVLVAMSGGVDSSVAAARLVEQGYDVIGATMK
                     LFCYGDSVPDRPCCSLDSINDARAVAHRLGIPHYVLNLEDRFDLHVIQNFVSEYGRGR
                     TPIPCVRCNSFTKFRDLLAHADSLDCQYLATGHYANARDGALYRGDDAAKDQSYFLWG
                     IDPRVVARMLTPVGALTKAETRAYARRLGLETADKPESVEICFVPDDDYVSVLEQHLP
                     ADAPALTPGPLVTVAGDVVGEHQGFARYTIGQRRGLPGGAGAPRYVVAIRPESREVVI
                     GGEEDLAGHRVVLEEVNWLARPLAAGATCGVQVRYRAPATPATVIARTNDSVTLALAA
                     PVRAITPGQSGVLYGAARQLLGGGVIA"
     gene            complement(2873..4054)
                     /locus_tag="VFU45_04990"
     CDS             complement(2873..4054)
                     /locus_tag="VFU45_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEU5358449.1"
                     /translation="MPKPDPIYLDHAATTPVRTEVLEAMLPYLRAEVFGNPSSAHRFG
                     RSARAGVETAKKQVAAAVGAEPNQVIFTSGGTEADNLAVIGGALAAKAAGRSLRAVVE
                     STAHKAILAAAHAVVHFGGEEELLPVDGNGQLDLAAVDAALARGPGVLSVMWVNNETG
                     AIQPVAEIGARCRAAGVTFHIDGVQAFGKVAFAFRDLPADLLTISGHKIGAPKGIGAL
                     LVKDRRAIEAIIHGGGQQYGLRPGTENVPGIVALGRAAELTAADWMAESRRIAGLRDR
                     LWAGIHAAVGDAVQHAGAGPRAHHVLSVGIPGADSETLLMHLDLAGIAVSAGSACSTG
                     AVEPSHVLVAMGVPHDLAVGTIRMSLGHETTEADIDRVLATFPGVVDKVRKLAGALGR
                     A"
     gene            complement(4249..4965)
                     /gene="bshB1"
                     /locus_tag="VFU45_04995"
     CDS             complement(4249..4965)
                     /gene="bshB1"
                     /locus_tag="VFU45_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEU5358450.1"
                     /translation="MPVDLLAIAAHRDDVELTCAGTLIRAADLGHRTGILDLTQGETG
                     TRGSATLRGEEAARAAQVLGVVERRNAGLPDAHLHNTEDARRAVVAELRHFRPRVVIL
                     PYPVGRHPDHRIASELARDACFLAGLAKYDAPGEPHRPFKILYSLAYREDPVKPTFVV
                     DISAQMARKMEAIRCYASQFDGAHSAGEIFPTGQDLYSLVETQSAHYGSLIRTRFGEP
                     FFTHETVQVDDVVGLEVQSM"
     gene            complement(5067..>6266)
                     /locus_tag="VFU45_05000"
     CDS             complement(5067..>6266)
                     /locus_tag="VFU45_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5358451.1"
                     /translation="LDQLRNNKLRSALTILGVVIGVTVVMAMASIVQGVRSQIFNAIE
                     VAGPSTFYVMRFFSQTPVNPDNLPPEIRIRPVLDRRDAAAVAHAESIAYAGLWIQLFS
                     RITRGAEHTQTITLFAADDHFMDIQGGTLLRGRLFSPAELHGAPVIILEADVAARLFG
                     QEDPLGKYVRAGDQELKVIGIYQKPGNIFEPPGQATAGAIPFETGYQRYRYDQTNALF
                     IAVRPRAGVDPTRAQDEAIVALRRARGLRPAAPNTFDLITQDQILATVGSLTSVFFLV
                     MVALSSVALLVGGIGVMAIMMVSVTDRTREIGTRKALGATRRMVLWQFLVEAATLTLA
                     GGVIGIAFGLLTGEVLKRVLAIEASVPVWSALLACGVSVLIGLVFGVIPANRAAKMDP
                     VEALRHE"
BASE COUNT          892 a         2126 c         2359 g          889 t
ORIGIN      
        1 cgtgtgggtc ttgagccccg ccacgccgta gctcacgtgc acgccggcgt cctccagccg
       61 gcgggcccag gtgatgttgt tctcctcgtc gaaccgcgcc tggagctcga tcagcaccgc
      121 cacctgcttg ccgcgctcgg cggcgtgctg cagtgcccgc gcgatgccgg catcggcccc
      181 ggtgcggtag agcgtgagtt tgatcgccac cacgtcggga tcctcggtgg cctcgcggat
      241 gaagcggtcg acggtggtgg cgaagctgtc gaacgggtgg tgcgccagga cgtcgccttc
      301 gcggagcgcc tcgaacaccg tccgcgcggc cgcgaagcgg ggcggggtgc cggggtggaa
      361 cgccgggtcg cgcagctccg gcacctcgat gccggcgagg cccaggaggt cgcccgcggc
      421 catgatgccc cgcacctcgt agatgtcctc gcccccgagc cacccggggg cctcctcggc
      481 cagttcgtcg aggagcatct ggcggatggc gaccggcgtg cgggggctca cctcgacccg
      541 cgtgacctcg ccgaaccgcc ggttgcgcag ctcctcctgg atgaggctca gcaggtcctc
      601 cgcctcttcc gggtcgatct gcacgtcggt gttgcgggtc acccggaagg tggaccaccc
      661 cacgatctcg acgccgggga agagcgcttc caggttggcg ccgatcacct gctccagcgg
      721 cacgaaccga tgtccggcgc ccaggggcac ccagcgcggg aggagccgcg gcaccttgac
      781 ccgggcgaac cgctcttcgc cttggtcgcc ccggagcatc accgccaggg agagtgacag
      841 gttggagatg tgggggaacg ggtgcgccgg gtcgactgcg agcggtgtga ggaccgggaa
      901 cacgttcgct tcgtaccatc ggtcgagcgc ctcccgctcc tccggcgcca cggcggtcat
      961 gtccatcagc tccacgccgc gggccgccag cgccggaaac agctcctcca ccaggcatgc
     1021 gtccgcccgg gcctgcatct cgcggaccgt ccggccgatg accgtgagct ggtcctccgg
     1081 agtcatcccg tccgccgggc gctccacgac gccggcggcc agctgctgct tgagccccga
     1141 gacgcgcacc tggaagaact catcgaggtt gctggcgtag atggcgagga acttcaggcg
     1201 ctcgagcagg ggcgtgcggg cgtcgagcgc ctggtggagc acccggcggt tgaactccag
     1261 ccacgacagc tcccggttga tgtagaggga cgggtggtgg aggtccggcg ccgtggggcg
     1321 ggagcgggcg ggagcggcgg aatcggtcat gcggggaagc tacccgggca ttgtaacagg
     1381 cccatggggg tcaatcgagc gtgtggatct cgacctccgg ggggcggcgc cgggggcggg
     1441 tggcggccag gagcagggcc gagctgatgc ccaccaccag gcctccccag aacgcgggcc
     1501 agaagcccgc gacgctgaac ccgagccccc agcgggtcga gagccacccg gtggcctcga
     1561 gcaggatggc attgaccacc agggtgaaga gcccgaaggt gaggaggatg agcgggcagg
     1621 tgaggatcga gagcagcggc ttgagcaccg cgttcacgat gccgaacacg gcggccacga
     1681 ggaagagccg aaggactccg ccgtcggagg tgtcgaagtg cagcccgggg acgatcgcga
     1741 tggcaaggaa gagtgccgcg gcggtcactg cccagcggag cgcgagctcg cgcatcaggc
     1801 gatcacgccg ccgccgagga gctgccgggc ggcgccgtag agcacgcccg actggccggg
     1861 cgtgatggcc cggaccggcg ccgcgagcgc gagggtgacg gagtcgttcg tccgcgcgat
     1921 gaccgtggcc ggtgtggcgg gggcccggta gcggacctgg acgccgcagg tcgcgcccgc
     1981 cgcgaggggc cgggcgagcc agttgacctc ctccaggacg acgcggtggc cggccaggtc
     2041 ctcctccccg ccgatgacca cctcgcggga ttccgggcgg atcgcgacca cgtagcgcgg
     2101 cgcgccggcg ccgccgggga ggccgcgtcg ctggccgatg gtgtagcggg cgaagccctg
     2161 gtgctcgccg accacgtcgc cggcgacggt gaccagcggg ccgggggtga gggccggcgc
     2221 atcggccggg aggtgctgtt ccagcacgct cacgtagtcg tcgtcgggta cgaagcagat
     2281 ctcgaccgac tccggcttgt cggcggtctc gagcccgagg cgccgggcgt aggcgcgggt
     2341 ctcggccttg gtgagcgccc cgaccggcgt cagcatccgc gcgaccaccc tcgggtcgat
     2401 gccccagagg aagtagctct ggtccttcgc cgcgtcgtcg ccgcgataga gggcgccgtc
     2461 gcgcgcattg gcgtaatggc ccgtcgcgag gtactggcag tcgagcgagt cggcatgggc
     2521 cagcaggtcg cggaacttgg tgaaggaatt gcaccggacg cacgggatcg gggtgcggcc
     2581 ccggccgtac tcgcttacga agttctggat gacgtggagg tcgaagcggt cttcgaggtt
     2641 gagcacgtag tgcgggatgc cgagccggtg cgcaaccgca cgcgcgtcat tgatcgagtc
     2701 gagggagcag caggggcggt ccggcaccga gtcgccgtag cagaagagct tcatcgtggc
     2761 gccgatgaca tcgtatccct gctcgaccag gcgcgccgcg gcgacggagc tgtcgacgcc
     2821 gccggacatc gcgacgagca cccgcgggcg tccggcggga ccggcgaggg cctcaggcac
     2881 gacccagcgc cccggccagc ttccgcacct tgtccaccac cccggggaag gtcgcgagca
     2941 cccggtcgat gtccgcctcg gtcgtctcgt ggcccaggct catccggatg gtccccacgg
     3001 ccaggtcgtg cgggacgccc atcgccacga ggacgtggga gggttcgacc gcgccggtgg
     3061 agcaggcgga gcccgccgag accgcgatgc cggcgaggtc gaggtgcatc agaagcgttt
     3121 cgctgtcggc gcccgggatg ccgacgctga gcacgtggtg ggcgcggggc cccgcgccgg
     3181 cgtgctggac cgcatcgccc accgcggcgt ggatcccggc ccagagccgg tcccgcagcc
     3241 ccgcgatgcg tcgcgactcc gccatccaat ccgcggcggt gagttccgcg gcgcggccca
     3301 gcgccacgat gccggggacg ttctcggtcc cggggcgcag gccgtactgc tggcccccgc
     3361 cgtggatgat cgcctcgatg gcgcggcggt ccttgacgag gagggcgccg atgcccttgg
     3421 gggcgccgat cttgtggccg gagatggtga ggaggtcggc ggggaggtcg cggaaggcga
     3481 aggccacctt gccgaatgcc tgaacgccgt cgatgtggaa ggtgacgccc gccgcccgac
     3541 atcgcgcgcc gatctccgcg accggctgga tggcgccggt ctcgttgttg acccacatca
     3601 ccgagagcac gcccggtccg cgcgcgagcg ctgcgtccac cgcggcgagg tcgagctgtc
     3661 cgttgccgtc gaccgggagg agttcctcct ccccgccgaa gtgcaccacc gcgtgggccg
     3721 ccgccaggat ggccttgtgg gccgtggact cgaccaccgc gcggaggctc cgccccgccg
     3781 ccttggccgc gagcgcgccg ccgatgacgg caaggttgtc cgcctcggtg ccgccgctcg
     3841 tgaagatgac ctgattgggc tccgcgccga ccgccgccgc cacctgcttc ttggcggtct
     3901 cgaccccggc gcgcgcgctc cggccgaatc ggtgggcgct cgagggattg ccgaacacct
     3961 ccgctcggag gtacggcagc atggcctcca gcacctcggt gcggaccggg gtggtggcgg
     4021 cgtggtcgag gtagatgggg tcgggcttgg gcatgctgga aaataggcgg tcgggcggta
     4081 agggggtaag gcggttgggg gtatgcctcc cgcaggatcg cgcccgccag cgccgaaccc
     4141 cgggatccgc gaccgttgga ccgccttccc ccgccttccc ccgccttcct gctccgtctc
     4201 cttctccttc tccttctcct tctcctcccc tccgtcccgc aggcccgcct acatcgactg
     4261 cacctcgagt cccaccacat cgtccacctg gaccgtctcg tgggtgaaga agggctcccc
     4321 gaaccgggtg cggatgagcg agccgtagtg ggccgactgg gtctcgacca gcgagtacag
     4381 gtcctggcca gtggggaaga tctcgccggc ggagtgggcg ccgtcgaact gggaggcgta
     4441 gcagcggatc gcctccatct tccgtgccat ctgggccgag atgtcgacga cgaaggtcgg
     4501 cttgaccggg tcttcgcggt aggcgaggga gtagaggatc ttgaacggcc ggtggggctc
     4561 acccggggca tcgtatttgg cgagcccggc aaggaagcag gcgtcacggg ccagctcgga
     4621 agcgatccgg tggtcggggt ggcggcccac cgggtagggg aggatcacca cccgcgggcg
     4681 gaaatgccgc agctcggcca ccaccgcccg ccgggcgtcc tccgtgttgt ggaggtgcgc
     4741 gtccgggagg ccggcgttcc ggcgctcgac cacgcccagc acctgggccg cccgcgccgc
     4801 ctcctcgccg cggagcgtcg cgcttccgcg ggtcccggtc tcgccctggg tcaggtcgag
     4861 gatcccggtg cggtggccca ggtcggcggc gcggatgagc gtgcccgcgc aggtgagctc
     4921 gacgtcgtcg cgatgggcgg cgatggccag gaggtcgacg ggcatgttcg ctccgcgaag
     4981 gtgaacggta aacggtaaac ggtgaagggg ggctccggga ggcatgcggt ccccgttccc
     5041 ggttcactgt tcaccgttca ccgccgtcac tcgtgccgca gcgcctcgac cggatccatc
     5101 ttggcggcgc ggttggcggg gatgacgccg aagacgaggc cgatcagcac gctcacaccg
     5161 caggcgagga gcgcgctcca caccgggacc gacgcctcga tggcgagcac ccgcttgagg
     5221 acctcgccgg tgaggaggcc gaaggcgatg ccgatgacgc cgcccgccag ggtgagggtg
     5281 gcggcctcga ccaggaactg ccacaggacc atgcgccggg tggcgcccag cgccttgcgg
     5341 gtgccgatct cgcgggtgcg gtcggtgacc gacaccatca tgatcgccat cacgccgatc
     5401 ccgcccacca ggagcgccac gctcgacagg gccaccatca cgaggaagaa gaccgacgtc
     5461 aggctgccga cggtggccag gatctggtcc tgggtgatca ggtcgaaggt gttgggcgct
     5521 gcgggccgga gcccccgcgc ccgccggagc gcgacgatgg cctcatcctg ggcccgggtg
     5581 gggtcgaccc cggcccgggg gcggaccgcg atgaagagcg cgttcgtctg gtcgtagcgg
     5641 tagcgctggt agccggtctc gaacgggatg gccccggccg tcgcctggcc ggggggctcg
     5701 aagatgttgc cgggcttctg gtagatcccg atgaccttga gctcctgatc ccccgcccgc
     5761 acgtacttgc cgagagggtc ctcctggccg aagagccggg cggccacgtc cgcctccagg
     5821 atgatgaccg gcgcgccgtg gagttccgcc ggtgagaaca gccggccgcg cagcagggtg
     5881 ccgccctgga tgtccatgaa gtggtcatcg gcggcgaaga gcgtgatggt ctgggtgtgc
     5941 tccgcgccgc gggtgatgcg gctgaacagc tggatccaga gcccggcgta ggcgatcgac
     6001 tcggcgtgcg ccaccgccgc cgcgtcgcgc cggtccagca ccgggcggat ccggatctcg
     6061 ggggggaggt tgtcgggatt gaccggggtc tgggagaaga accgcatcac gtagaaggtg
     6121 ctgggccctg ccacttcgat ggcgttgaag atctgcgagc gcaccccctg gacgatgctg
     6181 gccatcgcca tcaccaccgt gacgccgatg accacgccca ggatcgtcag ggcggagcgg
     6241 agcttgttgt tccgcagctg gtcgag
//
LOCUS       DASUAP010000199         6320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_46814, whole genome shotgun sequence.
ACCESSION   DASUAP010000199 DASUAP010000000
VERSION     DASUAP010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46814"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1052)
                     /locus_tag="VFU45_05005"
     CDS             complement(72..1052)
                     /locus_tag="VFU45_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358452.1"
                     /translation="MPHRQLTVSGLPAISLSNDEIEVVCVPSLGGKLTNLRRHRGREW
                     FWRNPAIPFAPPVWGASYIETADSGGWDECFPTIGPSAMPGALPGEPPLPDHGELWAL
                     PWTHAVIERPDGVTLIGHVEGRLLPYDLRREVTVAVDQPEVRIGYTLRHRGEVAFPFI
                     WAAHPLLNVQKGSTIALPTVERAYVISVAGRTDLAVGDAISWPLEGSGAAWTFPGARG
                     WALMVGADLGASGRMEVTDPLRGERLQVDASPEVGQVGVWINAGGWAPATLGGRAGPA
                     PYVNLGLEPCIGAPDRLDLAVERWRAAQTLAPGEERRWSLTIRLPGPAGD"
     gene            1176..1574
                     /locus_tag="VFU45_05010"
     CDS             1176..1574
                     /locus_tag="VFU45_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU5358453.1"
                     /translation="MSPPVRRFEDLLVWQHARSLSVTIARFSAQSLRTEPDLADQMKR
                     AARSVCANIAEGFERNGRREFDQFLGIARASCGEVRSFLYDALDEGHLTESTFDTLKA
                     QAELVSRMLYALQRSVRQQRGRKSPAGAPT"
     gene            complement(1621..2835)
                     /locus_tag="VFU45_05015"
     CDS             complement(1621..2835)
                     /locus_tag="VFU45_05015"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU5358454.1"
                     /translation="MTETYFRRGFGLKSAIEGQRTADYHSEVVDALRSHGHALTVGPL
                     TFRLAREFGFCYGVDRAVDYAYETRHKFPDRRIFLAGEIIHNPHVNQKLEAMGIGFLH
                     RDAGGDFDFSGIGAADVVIMPAFGVTLRDFARLQEIGCVRVDTTCGSVLNVWKRVEGY
                     AKDGFTAVIHGKYYHEETKATASQVERYPGGRYLVVRDMAEARPVLDFIEGRGDPAAL
                     RDRFAHASSAGFDFATDLVRAGIANQTTMLSGESLAIAAEFRKSFALRYGEAQVDAHF
                     RTFDTICSATQERQDAVVKLLEDRIDLMLVVGGYNSSNTCHLAALAQSKGVPTFHIED
                     AHCIEPADGTLRHQPVGQKREVTEPAGWLAGRTVVGITAGASTPNNKVGETIGRVCEV
                     AGVLGELERVIA"
     gene            complement(2832..3401)
                     /locus_tag="VFU45_05020"
     CDS             complement(2832..3401)
                     /locus_tag="VFU45_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358455.1"
                     /translation="MRHSRFPPRLMRAAASSALACAALACGPRGGRVSARVSGAAFQT
                     RLAGPAQASVCRSRGVLVLEGSEGDDGLAMVWFFGDSLKADSVPVGPPIWRRPSSTDS
                     LRSTASGAYRRTTDIDVMGYQTRNGWLRVTPGQDGTVAAAFAAVFDRTGAAESLWVTG
                     RFDGVRPQDDSTLCRIPRAAPDSGVTLGQ"
     gene            complement(3448..5601)
                     /locus_tag="VFU45_05025"
     CDS             complement(3448..5601)
                     /locus_tag="VFU45_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358456.1"
                     /translation="MGALLLALLLAAPPGADTLPPFADAATARVVAHAIARQSDQDTL
                     VSDYSARLHYRLSVAFGRRRWARIPAFGVEEQDVLVHWQRPNDIRVDVLGQRERSRSP
                     DADISSGFDRPWFIPRALGDSVRILDSDFPARAALHPFAPDGPAWYRYALTDSVRIGD
                     GRGGMITLLGITVVPRRDGPALVAGTIWVDEASWDVARFSFRFVGTGLWAVPEHPTHA
                     DSSDAVRDNRLANRILTVDVDLEYALEDRRYWMPYRQVLSGQVVIPFVGDIVIPFTVS
                     TTFDDYAINTGRTIAFALPLPDTSSADPHRSRKDNDRTEIQDSTGRRDVAGRLEGGGR
                     YELHVPPRDSLRAYAGWTDSLALDRADDQRADLREVQRQLADLAEDLPREMTGAPGAG
                     PALEHLADAFRYERVQGLALGYGWKAPIPGIRFTSVYGAARYGFSDDRITGRVELIRE
                     SPAGRWTLRGFREVAPVDRAFPPGELANSVNALFTAHDAADRALTQGGELRYETSIAM
                     GADLSLAGRMDDERNVSTRARSAVNDVLGGSGRFPPTPPVAEGVFGGVTARLDVRRGT
                     TSWMAGADVLSGGAGATAKVFGEWRAPLWSAERAPALTLRGGMATAHPLPQSQFRIGG
                     PWTARGFTYGTALGAAFWAAQLDVPLARGAIRPVAFADAAEAGSLDGVLRAPILTDAG
                     LGLSFLGGAVRFDLSQPLTRGAHGPRFDLVFGAAR"
     gene            complement(5583..6038)
                     /locus_tag="VFU45_05030"
     CDS             complement(5583..6038)
                     /locus_tag="VFU45_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5358457.1"
                     /translation="MKTPLPTPIPSVFSPLAKALLDGFSEGVIVFDREGKVLYANQQA
                     REALQDQFDLAEGAADLMPRLAALGGRLRPLRAGTLELGEAMFIPGIEGPTTLAAREK
                     EAIVRSLDAHGWKLAETAKHLGISRTTLWRRLKAYGLHRDGRSRWAHSS"
     gene            complement(6138..>6320)
                     /locus_tag="VFU45_05035"
     CDS             complement(6138..>6320)
                     /locus_tag="VFU45_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358458.1"
                     /translation="AYIYLQPLLTAAVAPLVLQGEAVTTRVVVGGLGIFAGLGLVIWG
                     ERAQRGSIPVASLPGE"
BASE COUNT          984 a         2301 c         2172 g          863 t
ORIGIN      
        1 atcgccgctg cggcgtcgct ccgggaggtc gacatggtag ctgatatagt actcgtcgcg
       61 gcccggccgc gctaatcgcc cgcggggccc ggcagccgga tcgtgagcga ccagcgacgc
      121 tcctcgcccg gcgcgagtgt ctgcgccgcc cgccaccgct ccaccgcaag gtcgagccgg
      181 tccggcgcgc cgatgcacgg ttccaggccg aggttcacgt acggcgccgg ccccgcccgg
      241 ccacccagcg tggcgggggc ccaccccccg gcattgatcc atacccctac ctgccccacc
      301 tcgggcgacg catcgacctg gagccgctcg ccacgcagcg ggtcggtcac ctccatccgt
      361 cccgacgcac cgaggtccgc cccgaccatc agcgcccacc cccgggcgcc cgggaaggtc
      421 cacgcggcgc ccgatccctc gagcggccag gagatggcgt ctcccaccgc aaggtcggtg
      481 cggcccgcca ccgagatgac gtaggcgcgc tccaccgtcg gcagggcgat ggtactgccc
      541 ttctgcacgt tgaggagcgg gtgcgcggcc cagatgaagg ggaacgccac ctcgccccgg
      601 tgccgcaggg tgtacccgat ccgcacttca ggctggtcga cggccaccgt gacttcacgt
      661 cgcaggtcgt acggcaggag ccgcccttcc acgtgcccga tgagcgtcac gccgtccggg
      721 cgttcgatga cagcgtgggt ccacggcaac gcccacaact cgccatggtc gggcagcggc
      781 ggctcccccg ggagcgcgcc cggcatcgcc gagggcccga tcgtcgggaa gcactcgtcc
      841 cagcccccgc tgtccgctgt ctcgatgtac gacgcgcccc acaccggcgg ggcgaacggg
      901 atcgccgggt tccgccagaa ccactcccgc ccccgatgcc gccgcaggtt ggtgagcttc
      961 ccgcccaggc tcggcacgca cacgacctcg atctcgtcgt tcgagagcga gatggcggga
     1021 aggccggaga cggtaagctg gcggtggggc acgggtccga ggccaacggg taagggaaga
     1081 gggaacaggc gggtggaatg cgagaatgcg gaaagacgat gcgctccgca cggaaaggat
     1141 gccggtctag acggaaatcc atggcatcgt tcgctatgag tccgcccgtc cgacgattcg
     1201 aggaccttct ggtgtggcag cacgcacgat cgctgtccgt caccattgcc agattcagcg
     1261 cccagtcact ccgaacggaa ccggacctgg cagaccagat gaagcgcgcc gcacgatcgg
     1321 tgtgcgccaa cattgccgaa ggctttgagc gcaacggtcg tcgggagttc gatcagttcc
     1381 tggggatagc ccgcgcgtcc tgtggcgagg tccgatcatt cctttacgac gccctggacg
     1441 aggggcacct caccgagagc acgttcgata cgctcaaagc gcaggcggag ctggtcagtc
     1501 ggatgcttta cgcgctccag cgatctgtcc gtcagcagcg cgggcgtaag tccccagcgg
     1561 gcgcgccaac gtgaccacct cccaccttcc cgcccacttg caccatggcc cctgcgcctg
     1621 tcatgcgatg acccgctcca gttcgccaag cacccccgcc acctcgcaca cccgcccgat
     1681 cgtctcgccc accttgttgt tgggggtcga ggcccccgcc gtgatcccga ccaccgtccg
     1741 gcccgcgagc caccccgccg gctcggtcac ttcccgcttc tggcccaccg gctggtgccg
     1801 caacgtgccg tcggccggct cgatgcagtg ggcatcctcg atatggaagg tcgggacgcc
     1861 tttcgactgg gccagtgccg ccaggtgaca ggtgttgctc gagttgtagc cgcccaccac
     1921 cagcatcagg tcgatccggt cctcgaggag cttcaccacc gcgtcctggc gctcctgcgt
     1981 cgccgagcag atggtgtcga aggtgcggaa gtgggcatcg acctgtgcct cgccataccg
     2041 gagggcgaag ctcttccgga actcggcggc gatggccagc gattcccccg agagcatcgt
     2101 cgtctggttg gcgatcccgg cccgcaccag gtcggtggca aagtcgaatc ccgctgaact
     2161 ggcgtgggca aagcggtcgc gcagcgccgc cggatcgccg cgcccctcga tgaagtcgag
     2221 caccggccgc gcctccgcca tgtcgcggac caccaggtac cggccgcccg gatagcgttc
     2281 cacctggctc gccgtggcct tcgtctcctc atggtagtac ttcccgtgga tcaccgcggt
     2341 gaagccgtcc ttggcgtacc cctcgacccg cttccagacg ttgagcaccg agccgcaggt
     2401 ggtgtccacc cggacgcagc cgatctcctg caggcgcgca aagtcgcgga gcgtcacgcc
     2461 gaatgccggc atgatcacca cgtcggccgc gccgatgccg gaaaagtcga agtcgccgcc
     2521 ggcgtcccgg tgcaggaagc cgatgcccat cgcctcgagc ttctggttca cgtgcggatt
     2581 gtggatgatc tcgccggcga ggaagatgcg gcggtcgggg aacttgtggc gcgtctcgta
     2641 ggcgtagtcc accgcgcggt cgacgccgta gcagaacccg aactcccggg ccagccggaa
     2701 cgtgagcggg ccgaccgtga gcgcgtggcc gtgcgaccgc aaggcgtcga ccacctcact
     2761 gtggtaatcg gcggtgcgct gcccctcgat cgcggacttg agcccgaatc cgcggcggaa
     2821 ataggtctcc gtcattggcc caaagttaca cccgagtcgg gtgccgcgcg cgggatccgg
     2881 cagagcgtgg aatcgtcctg cgggcgcacg ccatcgaacc gccccgtgac ccacagcgat
     2941 tcggcggcgc cggtgcggtc gaacacggcc gcaaaggcgg cggccaccgt accatcctgc
     3001 cccggcgtga cgcggagcca tccgttgcgg gtctggtacc ccatcacgtc gatgtcggtc
     3061 gtgcggcggt aggcgcccga cgccgtcgag cgcagcgagt cggtcgacga cggccgccgc
     3121 cagatcggcg ggcccaccgg aacggagtcg gccttgagcg aatcaccgaa gaaccacacc
     3181 atcgccaggc cgtcgtcccc ctcggacccc tcgagcacca ggaccccgcg cgatcggcag
     3241 accgacgcct gggccggccc ggcgagccgc gtctggaacg ccgcgcccga cactcgggcc
     3301 gacacccgtc cgccgcgcgg gccacaggcg agcgccgcgc acgccagcgc gctcgacgcc
     3361 gcggcgcgca tcaatcgagg agggaagcgg gagtggcgca tgaggtctgc gggaaggtac
     3421 cccggcatcg gcccgcgggt caaagcgtca gcgcgccgcg ccgaagacga ggtcgaacct
     3481 cggcccgtgc gcgccccgcg tcagcggctg gctcaggtcg aaccgcacgg cgccgcccag
     3541 gaacgagagg cccaggccgg cgtcggtcag gatcggagca cgaaggacgc catcgagcga
     3601 ccccgcctcc gccgcgtcgg cgaacgccac cggccggatg gcgccgcggg ccagcggcac
     3661 atcgagctgc gccgcccaga acgccgcgcc cagcgcggta ccgtacgtga acccgcgcgc
     3721 cgtccacggg ccgccgatcc ggaactgcga ttgcggcagc ggatgcgcgg tcgccatccc
     3781 gccgcggagc gtcagcgccg gtgcccgctc ggccgaccag aggggggcgc gccactcgcc
     3841 gaacaccttc gccgtggccc ccgcgccgcc cgaaaggacg tccgcgccgg ccatccacga
     3901 cgtggtgccg cggcgcacat cgagccgcgc cgtcacgccg ccgaagacgc cctccgccac
     3961 cggtggcgtg ggcgggaagc gcccggagcc gcccagcacg tcgttcaccg ccgaccgcgc
     4021 gcgggtgctg acgttccgct cgtcgtccat tcgtccggca agcgacaggt ccgctcccat
     4081 cgcgatcgac gtctcgtagc ggagctcgcc gccctgcgtc agcgcccggt cggcggcatc
     4141 gtgggccgtg aagagcgcgt tgaccgaatt ggcgagctcg cccggcggga acgcgcgatc
     4201 caccggcgcc acctcccgga acccgcgcaa cgtccagcgc cctgcgggag attcccggat
     4261 cagttcgacg cgccccgtga tccgatcgtc gctgaatccg tagcgtgccg cgccgtacac
     4321 cgacgtgaac ctgatccccg ggatcggcgc cttccagccg tagccgagcg cgagcccctg
     4381 cacccgctcg taacggaagg cgtcggccag gtgttcgagc gcggggccgg ccccgggggc
     4441 acccgtcatc tcccggggca ggtcctccgc gaggtcggca agctggcgct gcacctcacg
     4501 gaggtccgcc cgctggtcgt cggcgcggtc gagcgccagg gaatcggtcc agccggcgta
     4561 cgcgcgcagg gagtcgcgcg gcggcacgtg cagctcgtag cgcccacccc cctcgagtcg
     4621 gccggcgacg tcgcggcgcc cggtcgagtc ctggatctcc gtcctgtcgt tgtccttgcg
     4681 cgatcgatgc gggtcggcgg acgacgtgtc gggaagcgga agggcaaagg cgatcgtccg
     4741 tccggtgttg atggcgtagt cgtcgaaggt ggtgctcacc gtgaagggga tcacgatatc
     4801 gcccacgaac gggatcacga cctggccgga gagcacctgc cggtagggca tccagtagcg
     4861 ccggtcttca agtgcatact cgaggtcgac gtcgacggtg aggatccggt tggccaaccg
     4921 attgtcccgg acggcatcgg acgagtcggc gtgggtcgga tgctccggca ccgcccagag
     4981 cccggtgccg acgaaccgga aggagaaccg cgccacgtcc caggacgcct cgtccaccca
     5041 gatggtcccg gccaccagcg ccggcccgtc gcggcgcggc accacggtga tgccgaggag
     5101 cgtgatcatc ccgccccgcc cgtcgccgat ccgcacgctg tcggtcaggg cgtaccggta
     5161 ccaagcgggc ccgtcgggcg cgaaaggatg gagcgccgcc cgcgccggaa agtcgctgtc
     5221 gaggatccgg accgaatcgc cgagggcacg gggtatgaac cacggccggt cgaagccgct
     5281 gctgatgtcg gcgtccggag agcgggagcg ctcgcgctgc cccagcacat cgacccggat
     5341 gtcgttgggg cgctgccaat ggaccaggac gtcctgttcc tcgaccccga aggccgggat
     5401 gcgcgcccaa cggcggcgac cgaaggccac cgacaggcgg tagtgcaggc gggcggagta
     5461 atcggagacg agcgtgtcct ggtcggactg gcgggcgatg gcgtgcgcga ccacgcgcgc
     5521 ggttgcggcg tcggcgaacg gaggcagcgt gtcggccccg ggcggcgccg cgagcaggag
     5581 cgctaggagg agtgcgccca tcggctacgg ccgtcccggt gcaggccgta cgccttgagc
     5641 cgccgccaga gcgtggtgcg gctgatcccg aggtgcttcg ccgtctccgc cagcttccag
     5701 ccgtgggcgt cgagcgaccg gacgatcgcc tccttttccc gcgcggccag ggtggtcggg
     5761 ccttcgatgc ccgggatgaa catcgcctcg ccgagttcca gcgtcccggc gcggagcggt
     5821 cggagccgcc cgccgagcgc cgccaggcgc ggcatcaggt cggccgcacc ttcggccagg
     5881 tcgaactggt cctgcagcgc ctcacgcgcc tgctggttgg cgtagagcac cttcccctcc
     5941 cggtcgaaca cgatcacgcc ctcgctgaac ccatcgagca gggccttggc gaggggagag
     6001 aagaccgacg ggatcggggt gggaagcggc gtcttcatgg tctcggacat gtgctgacgt
     6061 acgaaaaaag tggttccgga gtttcattcc ggtacgtttg ctaacgtatt gcacgacaat
     6121 gatttgtcca gtagcaccta ctccccgggg agggaagcca ccgggatcga cccgcgctgc
     6181 gcccgctcac cccagatgac cagcccgagc ccggcgaaga tcccgaggcc ccccaccacc
     6241 acgcgggtcg tcacggcttc cccctggagc accagcggcg ccacagccgc cgtcaggagc
     6301 ggctgcaggt agatgtaggc
//
LOCUS       DASUAP010000200        21641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_346521, whole genome shotgun sequence.
ACCESSION   DASUAP010000200 DASUAP010000000
VERSION     DASUAP010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 21641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21641
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346521"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFU45_05040"
     CDS             complement(<1..872)
                     /locus_tag="VFU45_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358459.1"
                     /translation="MNTRAAAAAILCAATLAAPSAASAQGWRVRLDANAQTVSYRGWQ
                     MDSIPSGSVVTGPGGGPTTPDGYAVACDGGAYCMFFRPGSRQASTPGVVDADATMWGL
                     GVSGLSLHTHARVGGDLGSNGTWPGPDRTFELVEGFADYDRGGWGLRLGRQITDGRLG
                     RYGFDGLTGTVRDRARTLTAQVYGGWGLGRAANVPVTSGALDPLNDFQPSRSQLFVGG
                     AARFSGTTGDLAAEYLRLTDALHYGIDQERVGVSGDARIPRTPLALTADVQYDLAAGL
                     WGSWDLGARWLARPV"
     gene            complement(869..2857)
                     /locus_tag="VFU45_05045"
     CDS             complement(869..2857)
                     /locus_tag="VFU45_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358460.1"
                     /translation="MIRSIGALRGVVHLFQGAAGVPTGQQAERIHEAVDSVVIRSPLP
                     DPIVKVAQFLFQQPPWLMAGGAVVGAIVGVVVLVWLWRRRRPIWAWIVARQGWVQGAM
                     IGAVVLVVALAGLGAHKSFDYVMHDNNFCRGCHIFIPSGQPFVRPDTGTYLLVNALEG
                     KHSKLSCHSCHPFNLEAQTKEMIGWILARPEVVPPHGKVPRAVCEQCHERGDAKKSWE
                     QISATAGHRIHLSSDSAVLKGKVECLTCHARSAHRFIPADTTCLDAGCHIGTKIRLGT
                     MQAAQTQMHCTLCHQFDVGVPALAERDSASKLLIPSGRECTACHQMRGLIAAFDFTRD
                     PHRALCGDCHDPHKQTKPADAAARCAQCHTEWKKIPFHTGAAHRKAVGPTTCINCHQP
                     HTARVDASDCTGCHREVRAPHKGEERLLPPIPFDTIKALGASPAPPGADPEPRGKGDA
                     PPPNAPPPHATPSAADSFSHARHRKLACLTCHDPGSQRKLTFEPPRGCQICHHQAPER
                     RTCSTCHDPGERAGMLSVAVPVGAPGRQAVPRPVAFPHARHDSIDCLRCHTTSVTLAA
                     DPAVLCASCHERHAGPTADCADCHVGEGLRTAHTPPIDAHRRCSGCHTSPAAAALTPA
                     RNLCLLCHAAQRTHEPGRECATCHLPGIAARWTGEDRP"
     gene            complement(2886..4445)
                     /locus_tag="VFU45_05050"
     CDS             complement(2886..4445)
                     /locus_tag="VFU45_05050"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU5358461.1"
                     /translation="MRRMSRTATLLTASAVLISTLVGCDQIVYRDRAPFNPPPDQTNG
                     FLGYYNTATKQTTCGNCHATTQGQWLSNSPHAKAWSALPANAAGYCKTCHTVSGNGNG
                     RDSAGYDKVPSTVYQDVQCESCHGPGQTHVSNPDGGTAPLAHMSLHDTLASCGACHSG
                     AHEPFVEQWAASGHGDSAANDHPATTAGCQGCHEGKAALARFGGSNVYAEASSGSTQT
                     ITCVVCHDPHGSPNTASLRLPITSPDPSVNLCMQCHWRSTTPSSTFNRSSYGAHAVQG
                     AVLLGSGAGWTPPGFAYDSAGAYTTHGSASNPLLCAGCHVHPFTVTDSNGFVFQSVGH
                     LFRPAPCVNGAGVPIADNSCAYTSTSRNWSSCTKSGCHADASVVANLFNNERATVAGL
                     VRQLWIDANHNGKLDPLPTDSGYLAQIYATTPTELTANTNGLTTAEGALFNAQMLAEA
                     SQYSHSDGSYGAHNPFFYEGLLSATINAVKTQYGLAPPPPAIQAQMDRALARPGVHYA
                     PPHTGGLASTR"
     gene            4648..5211
                     /locus_tag="VFU45_05055"
     CDS             4648..5211
                     /locus_tag="VFU45_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358462.1"
                     /translation="MLAAGGAVIFALGLGLGYLAAGDRGRAPAPVAESLAAGGAEAPP
                     DISNMSPRERFDRLYDRVMRAASSGDEATVQRFGPMAVAAYGMLDSVDDDARYDAALL
                     KIHLGDVAGAKALADTILQRTPTHLFGLMIRGTLARAAKDTVGARRNERAFLAAYNKE
                     MAKARPEYAEHKSALDAFRAQAQGDVK"
     gene            5291..5701
                     /locus_tag="VFU45_05060"
     CDS             5291..5701
                     /locus_tag="VFU45_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEU5358463.1"
                     /translation="MKAGTKFLLGAAVIVASVSVLMVEGVKETGTYFLTPTELKGKVA
                     ADPTFVNVGLKVGAKVVKGSLTRNTAAHEIDFKVSDGTTVYPVTYRGTIPDTFTDADD
                     IDVVVEGRLQADGVIHATDVLAKCGSRYDAVPKA"
     gene            5709..7751
                     /locus_tag="VFU45_05065"
     CDS             5709..7751
                     /locus_tag="VFU45_05065"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEU5358464.1"
                     /translation="MTLLGTFCLWATILVSTWGAVLAFSGRWRGRPELVGSIRGAVFA
                     GFGLLVVAAACLWQVLAAHDFNVEYVAAYTSRNLPTYFLVSAFWAGQKGSLLLWAIVL
                     SLFAALAQWLTAKRYADLLPYVAGVTCTVLVFFVLVMVTSANPFERLAYTPADGRGLN
                     PQLQHPAMIIHPPMLYLGYISAVIPFAFAMAALLSRRLDTGWIHAIRKWTLVSWLFLS
                     VGITLGMWWAYTELGWGGYWSWDPVENASFLPWLTMTAFLHSVMIQEKRGMLKRWNLS
                     LIIGTFLLTIFGTFLTRSGVIASVHSFTQSAVGYWFLGFLLLAGVASYTLLYTRWPQL
                     EAEVQLESMASREAAFLFNNLLLVGIAFSVLWGTLFPILSELVRGTKITVGPPFFNRV
                     NGPLGLLLLALTGIGPLIAWRRASTANLRRQFVWPLAAFGATFAGLLLAGVRQLSPLM
                     AWSLAGFVLGTVVQEFARGVRARHRMYHESPVVAFVHLVARNRRRYGGYLVHVAILIY
                     FVAFAGTAFREDLQVSLKPGQSVTMRSPFGHNYKFTHLGISQYDALNRSVSAATVEVS
                     EDGRPLGVLKSEKRQYVDGLGRPTFNPSTEIALRSTPQEDLYLVFESSVDGTEEANYR
                     FTITPLVWWVWYGGLMLVIGGLVTMWPASTGGGAGSKSPVEPGYAAALAASGGGAA"
     gene            7748..8362
                     /locus_tag="VFU45_05070"
     CDS             7748..8362
                     /locus_tag="VFU45_05070"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEU5358465.1"
                     /translation="MSAMDRRGFLGVLALGAVPLALHAAQQQQPGDTTRGQIPQGDAG
                     FLPNPALAGVSRPPVTAQENDPLIVGIEHRVRCPCPCGLDVYTCRTTDFTCSYSPERH
                     REIMGLVQQGMTPDQVVATLVAKYGESTLMAPKPRGFNLVGYLLPGSLVLLLGAALTS
                     VILSRRRGAAPAAGFVPAMAGDRTVDASPDELAKLAQAMRDLDQ"
     gene            8359..8892
                     /locus_tag="VFU45_05075"
     CDS             8359..8892
                     /locus_tag="VFU45_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5358466.1"
                     /translation="MNALEVILGVVVAAAAVTLVAWPLWHPPAPGVVTLDDAPSLEET
                     RKGVALLALREIEFDRETGKLSDQDYQRLKSAYTAEAVSALRVEEGRAARASAPAEAL
                     IAERVRALGARAGAQSAAGAGPSCSTCGPRPESDAIYCSNCGQMLVGHGCGRCGAALP
                     PGARFCESCGSPVAGAA"
     gene            complement(9102..9692)
                     /locus_tag="VFU45_05080"
     CDS             complement(9102..9692)
                     /locus_tag="VFU45_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358467.1"
                     /translation="MRRLLLCGALITLAPATLVAQQGAGTRLTLEFGAARFHGGVRDT
                     SATPVYIRPSRPTLMTLRLSGARFGVALTIAGSATGYSQGTIALTQGGDLTLVELAPE
                     VRHSLAATAAGGRLIIHAGPLLDLWKLNGGSSVARAGAHAGASLELPITERLGLSLRG
                     DAAITGSQLADTDLTPDLSQHTMWRTRVGIGAEYRL"
     gene            complement(9752..11011)
                     /locus_tag="VFU45_05085"
     CDS             complement(9752..11011)
                     /locus_tag="VFU45_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358468.1"
                     /translation="MRYSTLWLVPALLCVASACADDRGPTGASGPGTDVVVTEPAPTD
                     GEQGRLERLAKRTALALGDPSFRSLVRSELGASPFREHKLQFQRFLGRPGTAPMRAMA
                     AASGEPQTSIAADAADARPLEFYFPVHGQREAWQGDENILVATAINDHDTPVAYDTKG
                     RRHLLSPDRPPAVPVLAVEPQETDLDHPGAAPELTTCTTEVCGSSGGGTTGTTSGSGT
                     GGGTVMIHGLYMSYVQFSGTFEGWLKGDPEFELHILGPNAPGDNSTYHTLWCVGEHGN
                     TYWDLNSTTWSGDLMLFSQTQLNEYHTKYPDQNFSILALEDDDTACQVKVDNTNMAAF
                     VGAVNNFTSQYKGAKDNIGLNGATISAVKALWALIAEAINWIKSNDDLIGVAIANSIT
                     GYTHPVANWSFIGYDQARQGWIRLDMK"
     gene            complement(11169..11765)
                     /locus_tag="VFU45_05090"
     CDS             complement(11169..11765)
                     /locus_tag="VFU45_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358469.1"
                     /translation="MRARYDVTLMALLLAAAFPLSAQRPGTDESRPRQGFWFATGLGA
                     GREAEQCDGCAASGRQTGTVVHLAAGTTLTPDLRIGLDLTAWSRTLQGVGDQYDGTLT
                     AVVLYYPPGTGGLRLKLGAGASGMSVEPGSFVPASGFGFGGLAGVAYDLPLGRHVTIA
                     PEVTWFAGRPGDIKQGGSTMITGARFDVIAVGIDLTFH"
     gene            complement(11916..13637)
                     /locus_tag="VFU45_05095"
     CDS             complement(11916..13637)
                     /locus_tag="VFU45_05095"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358470.1"
                     /translation="MSAVEVTAGGREPWMPDLLRPKWQSARARLRQERKGAGTKVLLL
                     LVVGTVFWGGTYGLLHRVLRYLKDTPEVGPLIASKILAVGFLAFLSLLLLSNLITALS
                     SFFLARDLDLLISAPVDWLWFYLAKLAENLLHSSWMVVVMAVPIFAAYGAVFGGGPLF
                     PLVAAAALFPLLVIPAAVGAVLTLLLVNVFPARRLRDLLTLLAVAAAAGFVLWLRLAR
                     PEQLARPEGFRNLIDFLTLLQAPTSPLLPSQWAADTVMNWLDRVADPLPILLLWSTAG
                     AFVVLGALLHHRLYASGFSRAQEGGDTRDAGRRQARWVSALLRPLGAMQREMLLKDVR
                     VFFRDSTQWGQLILIGVLLVVYLFNISALPINTGERVPLFVTTLVIFLNQGLSGFVLA
                     AIAVRFIFPAVSLEGRQFWLLRSSPLDLRALLRSKYWTGTAPLLVLSLGLTVITNVSL
                     KSAPVILLMNVGTVAALTFAIAALALAFGAVYPQFDSENAAQIPTSFGGLVCMMTITG
                     LLVLVIVIEAGPVAGFLRDTQLDGHPGVAGIIIATGVVVAGCLAVAWTALRVALRTLV
                     QFDTEID"
     gene            complement(13634..14452)
                     /locus_tag="VFU45_05100"
     CDS             complement(13634..14452)
                     /locus_tag="VFU45_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358471.1"
                     /translation="MGRVPPARRRPAPHRVPGRRLIRLTDLTKRYGSFTAVDHISLHV
                     PRGELFGFLGPNGAGKTTTLRMIAGILQPTTGTVTIAGHDIAIEPVAAKQKLGFIPDR
                     PYVYDKLTGAEFLRFVAGLYRQEGADVEARMAELLDLFELTDWKDQLVEKYSHGMRQK
                     LIISSALIHRPEVIVVDEPMVGLDPKGQAFLKDLFRRFVDRGGTVLMSTHTLEVAEAV
                     CDRIAIIHHGRIAGVGTMHELREQTASHGMSLQEVFLKLTGTGEVGALEAVFGK"
     gene            complement(14389..15948)
                     /locus_tag="VFU45_05105"
     CDS             complement(14389..15948)
                     /locus_tag="VFU45_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEU5358472.1"
                     /translation="MSRGFVAAVILVAWVASLAWLAERHYGAPGGRSVASIQRVPPGA
                     AYYRVSWGARQVGLASVTVDTLSPTDSTPAEVRLSERLVIVLAGPHGPERHEFSSNTF
                     LTTRLGLRRTGIQVSGPEGKAVATVVTYGDSLLAGTLDSTGGPWRSLQRIDGPLLPLG
                     AFPIQLAYRERPSIGVTVSHPVFDPATLEYYAAGATVTGDSTFVIPDSVIKVDTGYRV
                     ARTDTLRAWRVAMVQHGVPTHAWIDINGFPVRSWTGGGLTFERTAFEFTNDGLKALSD
                     SAHPPIGLPSLPATAGPADSALAAGNIGWRVVLPGAEYSDLQGAGPMQSFAGDTLALT
                     RMPFAPLRDPMRPLQPIPMTDLRFVGVLRGEPRLSPDDAAVTALARSVVGTETSARFA
                     TASLVRWIHRNIALVDDSAGGPWPTAAQVLARRRGNAAGLAILFTALARRVGLPARPV
                     AGLLIGRGGVRLHTWAEAFIGDWVPVDPAFGQFPASPAHARLVVGATSHWAEYLPLAG
                     ALRPTAFLDGD"
     gene            complement(15945..16451)
                     /locus_tag="VFU45_05110"
     CDS             complement(15945..16451)
                     /locus_tag="VFU45_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEU5358473.1"
                     /translation="MIEVRVDDLAFAEVDAVIRPADAALDPVTTAAARLDRQAGPRFS
                     EQRRTQQALVPGAAVVTGAGDLPAKFVVHAVIQSAEQPATADTVRRALVSAWQRVAQW
                     QLVSIAMPLVGAGAGQLSIDEAARLLRDTLDDPLRPAEYPASVVVMVEREGDRAEIEA
                     ALGGRRAP"
     gene            complement(16448..17245)
                     /locus_tag="VFU45_05115"
     CDS             complement(16448..17245)
                     /locus_tag="VFU45_05115"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5358474.1"
                     /translation="MRRFRQSRSADRDPALHDLFTPDGMEGEPYPSRADVRFPLRPLA
                     AGLPSDDIVVTDPLAEGSESDLLFRRAAEQVARQRPSDAVITYRSLLELDEHHVEARA
                     ALAAILEAQEDLDGAVDQLTLALADRPTDPGLLCQRGAVLGLLKRYAEAEADLRRALR
                     ESPEHPASLFQLGLLLHRKGLPAEAVETLQHAIHVDPANAPAQFYLGEALHHQGDLAG
                     AEAALRRAAELDLRQGRAYHLLGRILDRQGRSEEAREMYRRAREAAR"
     gene            17411..20329
                     /gene="uvrA"
                     /locus_tag="VFU45_05120"
     CDS             17411..20329
                     /gene="uvrA"
                     /locus_tag="VFU45_05120"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU5358475.1"
                     /translation="MAQDSLVVRGAREHNLQNVSVTIPRDKLTVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGLMEKPDVDAIEGLSPAISIEQKSASQNPRSTVGT
                     VTEIYDYLRLLWARTGVPHCPNDGTPITRQSASQITDTILGWPEGTRIEVLAPLVRGR
                     KGEFRELFEDARRQGFARVRVNGTTYELDQVPALERRKNHDIAVVVDRLVVRQSDRGR
                     LNDSVETALRAADGVVEVVEHGLRSAVSGEKGAGSATRDPRPTTKSHIFSEKFGCPVC
                     GLSLPELEPRQFSFNSPFGACPDCHGVGTRREVNADLVLGDASISILEGVILPWGEPS
                     GYLRKVVLPTLAKAFKFDLNAPWRDHSAAAKHAILHGEPGRVLKFAAEGSKKAEYESD
                     WEGVLRNIERRYRETTSDSIRVELEDFMVEQPCQTCHGRRLKPESLSVTVGGKGIGEV
                     VEMPVDQALGFFGGLGAKADGKRDKGKGNGGLDAEVSGPILKEVVDRLRFLRDVGLAY
                     LTLGRGAASLSGGEVQRIRLATQIGSRLVGVLYILDEPSIGLHQRDNERLLSTLRELR
                     DLGNTVIVVEHDEDTIRAADHVIDLGPGAGKLGGKVVAEGTVQEILRHPTSLTARYLR
                     RELRVRVPPKRRPGSGERLKIIGASGNNLKDLTVDVPLGKFVAVTGVSGSGKSTLVTD
                     ILYQALARHFYRAKVVPGQHKRIEGLDKIDKVIDIDQSPIGRTPRSNPATYTGLFTPI
                     REVFTQLPEAKIRGYGPGRFSFNVKGGRCEACQGDGLVKIEMHFLPDVYVPCEVCKGR
                     RYNRETLEVRFKGRSIADVLDLTVADAREFFEAQPRIAEKLELLNDVGLGYIHLGQAA
                     TTLSGGEAQRVKLATELSKRDTGRTLYILDEPTTGLHFEDVRLLLEVLHRLVDKGNSV
                     VVIEHNLDVLKTADWIIDLGPEGGEGGGRIVAEGTPEQVAATKGSFTGEFLKRTLAEA
                     "
     gene            20394..20609
                     /locus_tag="VFU45_05125"
     CDS             20394..20609
                     /locus_tag="VFU45_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358476.1"
                     /translation="MKYQDILDLLGYANGHDVAVRVTTSDGTEVVGIPTSVDTHVTAH
                     EVYLRPVGNDETEIGLSLGEIVAVELV"
     gene            complement(20646..21044)
                     /locus_tag="VFU45_05130"
     CDS             complement(20646..21044)
                     /locus_tag="VFU45_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU5358477.1"
                     /translation="MFGCHFPTGTVEPFESLVAWQKAHTLFLAVYRITAGWPRSELYG
                     MVSQARRAAFSVPANIAEGQARFGAPEFKRHVSIALGSLAELKYSLRAAKDLGWLADD
                     EYQRIHVLAEEASRCGYGLLRSIQDARTNK"
     gene            21125..>21641
                     /gene="sdaAB"
                     /locus_tag="VFU45_05135"
     CDS             21125..>21641
                     /gene="sdaAB"
                     /locus_tag="VFU45_05135"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU5358478.1"
                     /translation="MVSLLDIIGPVMVGPSSSHTAGACRIGLIARGMVGGTPEAAKVE
                     LHGSFARTGVGHGTDRAIAGGLMGFAPDDERLRESLELSEKAGLAIEFANVKLRGDHH
                     PNTTRIALTRGAVTATVIGSSLGAGRILITEIDGYPVEITGSYPVLVIVAHDEPGIVA
                     KVSGVLAAEQNN"
BASE COUNT         3219 a         7431 c         7707 g         3284 t
ORIGIN      
        1 accggacggg cgagccagcg cgcgccgagg tcccagcttc cccagaggcc ggccgcgagg
       61 tcgtactgca catcggccgt gagtgcgagc ggggtccggg ggatacgcgc gtcaccgctc
      121 accccgaccc gttcctggtc gatgccgtag tggagcgcgt ccgtgaggcg caggtactcc
      181 gcggcgaggt ccccggtggt gccggagaat cgtgccgccc cgcccacgaa gagctggctg
      241 cgggacggct ggaaatcatt cagcgggtcg agtgcgccgc tcgtcaccgg cacgttggcg
      301 gcgcgtccga gtccccatcc cccatagacc tgggcggtga gtgtgcgggc gcggtctcgc
      361 acagtgccgg tgagcccgtc gaagccatat cggccgagcc tgccgtcggt gatctgccgg
      421 ccgaggcgga ggccccagcc gccgcggtcg tagtcggcaa agccctccac gagttcgaac
      481 gtccggtcgg gcccgggcca tgtgccattg ctgccgaggt cgccaccgac gcgggcgtgg
      541 gtgtgaaggc tgagtccgga gacgccgagt ccccacatcg tggcgtcggc gtcgactacg
      601 ccgggcgtgc tcgcctgacg gcttccgggc cggaagaaca tgcagtaggc gccgccgtcg
      661 caggcgacgg cgtagccatc gggcgtggtc ggcccgccgc cggggccggt gaccaccgag
      721 ccggacggga tgctgtccat ctgccagccg cgatagctca cggtctgggc attggcatcg
      781 agtcggaccc gccagccctg ggccgatgcc gcgctcggtg ctgccagcgt cgcggcgcag
      841 aggatggcgg cggctgcagc gcgcgtgttc acggccggtc ctcgccggtc caccgcgctg
      901 cgatgccggg caggtggcac gtggcgcatt cgcgcccggg ctcatgggtt cgctgggccg
      961 cgtggcagag caggcacagg ttgcgtgccg gcgtgagggc ggccgccgcg gggctggtat
     1021 ggcatccgct gcatcggcga tgtgcgtcga tgggcggggt gtgcgccgtt cggaggccct
     1081 ccccgacgtg gcagtcggcg cagtcggcgg tggggccggc gtggcgctcg tggcacgagg
     1141 cgcagagcac cgccgggtcg gccgccagcg tcaccgatgt cgtgtgacat cgcaggcagt
     1201 cgatcgagtc gtgccgggcg tggggaaagg ccaccggccg cgggaccgcc tggcgccccg
     1261 gcgcaccgac cgggaccgcc acggacagca ttcccgcgcg ctcgcccgga tcgtgacagg
     1321 tgctgcaggt ccggcgttcg ggggcctggt ggtggcagat ctggcacccg cgcggcggct
     1381 cgaaggtcag cttgcgctgc gagccggggt cgtggcacgt aaggcaggcc agcttcctgt
     1441 ggcgcgcgtg tgagaacgag tccgccgccg acggtgtcgc gtgcggcggc ggagcattcg
     1501 gcggaggcgc gtcccctttg ccgcggggtt cggggtcggc cccgggtggt gcgggactcg
     1561 cgcccagcgc cttgatggtg tcgaacggga tgggcgggag caggcgctcc tcgcccttgt
     1621 ggggcgcccg gacctcacga tggcacccgg tgcagtcgct ggcatccacc cgggcggtat
     1681 gcggttggtg gcaattgatg caggtcgtgg gcccgaccgc cttgcggtgg gctgccccgg
     1741 tatggaaggg gatcttcttc cactccgtat ggcactgcgc gcagcgcgcg gcggcatcgg
     1801 ccggcttggt ctgcttgtgc gggtcgtggc agtcgccgca gagggcgcgg tgcgggtcgc
     1861 gggtgaagtc gaacgcggcg atgaggcccc gcatctggtg gcacgccgtg cattcgcgtc
     1921 cgctcgggat gagcagcttc gatgcggaat cgcgctcggc cagcgccggc acccccacgt
     1981 cgaactggtg gcacagggtg cagtgcatct gggtctgggc ggcctgcatg gtgcccaggc
     2041 ggatcttggt gccgatgtga cacccggcat cgaggcaggt ggtatccgcc gggatgaacc
     2101 ggtgggccga gcgcgcgtgg caggtcaggc actcgacctt gcccttgagg accgccgaat
     2161 cggacgagag atggatgcgg tgcccggccg tggcgctgat ctgctcccag ctcttcttcg
     2221 cgtcgccgcg ttcgtggcac tgctcgcaga ccgcgcgcgg caccttcccg tgcggcggga
     2281 cgacctcggg gcgcgccagg atccagccga tcatctcctt ggtctgggct tcgagattga
     2341 acggatggca cgaatggcag gaaagcttcg agtgcttccc ttcgagggcg ttcaccagca
     2401 ggtacgtgcc cgtgtcgggg cgcacgaacg gctgcccgct ggggatgaag atgtggcagc
     2461 cccggcagaa gttgttgtcg tgcatcacat agtcgaacga cttgtgggcc ccgagccccg
     2521 ccagggccac cacgagcacc accgcgccga tcatcgcccc ctgcacccac ccctgccgcg
     2581 cgacgatcca cgcccagatc gggcgccggc ggcgccagag ccacaccagc accaccaccc
     2641 cgacgatcgc ccccaccact gcacccccgg ccatcagcca ggggggctgc tggaacagga
     2701 actgggcgac cttgacgatc gggtcgggca ggggcgagcg gatgaccacc gaatccacgg
     2761 cctcgtggat ccgctcggcc tgctggccgg tgggcacgcc agcggcgcct tggaagaggt
     2821 ggaccacccc tcgcaaggcg ccgatggaac ggatcaacgg aatcctggcc tcccgggccg
     2881 acgggtcatc gcgtggaggc caggccgccg gtgtgcggcg gcgcgtagtg cacgcccggg
     2941 cgcgccaggg cgcggtccat ctgcgcctgg atggccgggg gcggcggtgc cagcccgtat
     3001 tgcgtcttca cggcattgat cgtggcgctc aggaggccct cgtagaagaa ggggttatgg
     3061 gcgccatagg aaccgtccga atgactgtac tggctcgctt cggccagcat ctgggcgttg
     3121 aagagggcgc cctccgcggt cgtgagcccg ttggtgttgg ccgtcaattc ggtcggggtg
     3181 gtggcgtaga tctgcgccag gtacccgctg tcggtcggca gcgggtccag cttcccgttg
     3241 tggttcgcgt cgatccaaag ctggcgcacc aatcctgcga ccgtggcccg ctcgttgttg
     3301 aagagattgg ccacgaccga cgcgtcggcg tgacatccgc tcttcgtgca gctgctccaa
     3361 ttgcgcgaag tcgaggtgta cgcgcaggag ttgtcggcga tcggtacgcc cgccccattg
     3421 acgcaggggg ccggccggaa gaggtgcccg accgactgga acacgaaccc attgctgtca
     3481 gtgacagtga acgggtgcac gtggcagccg gcgcagagga gcggattcga cgccgagccg
     3541 tgggtggtgt acgcgccggc cgaatcgtag gcgaagcccg gcggcgtcca cccggcgccg
     3601 gatccgagga gcaccgctcc ctggaccgcg tgcgctccgt aggaggaccg attgaacgtc
     3661 gacgaaggtg tggtgctccg ccagtgacac tgcatgcaca ggttgaccga cgggtccggc
     3721 gaggtgatcg gcaaccggag gctggccgtg ttgggcgaac cgtgcggatc gtggcagacc
     3781 acgcaggtga tcgtctgcgt actgccgctc gatgcttcgg cgtagacgtt ggagccgccg
     3841 aaccgcgcca gggccgcctt cccctcgtgg cacccctggc acccggcggt ggtggccgga
     3901 tggtcgttgg ccgcggagtc gccgtggccg gatgccgccc actgctccac gaacggctcg
     3961 tgcgccccgc tatggcaggc gccgcagctc gcgagcgtat cgtgcaggct catatgcgcc
     4021 agcggggcgg tcccgccgtc aggattgctc acgtgcgtct ggcccgggcc gtggcaggac
     4081 tcgcactgga cgtcctgata gaccgtggac ggcaccttgt catatccggc gctgtcccgg
     4141 ccattgccat tgccggagac ggtgtggcag gtcttgcagt accccgccgc gttggccggc
     4201 agcgccgacc acgccttcgc gtgggggctg ttgctgagcc actgcccctg cgtcgtcgcg
     4261 tggcagttgc cgcaggtggt ctgcttggtg gcggtgttgt agtagccgag gaagccgttg
     4321 gtctgatccg gcggcggatt gaacggcgcc cgatcgcgat agacgatctg gtcgcagccg
     4381 acgagggtgg aaatgagcac cgccgaggcg gtgagaaggg ttgctgtacg tgacatgcga
     4441 cgcatcgggg gctccgtggt ttcccggatg gcctatattc aagtgtctcc tcatttggcg
     4501 caagccgaaa aaggtgaaat gaccatgaaa ccagcttcag ggatgcattg ccccagctgc
     4561 ggccagcccg caagcgaaag tgcccgattt tgcgcgagtt gcggggcgac gttgcagctt
     4621 gcgccgcgga agggtccggg aaccttaatg ctcgcggctg gcggggcggt gatcttcgcc
     4681 ctgggcctcg ggctcggcta tctggcggct ggagaccgcg ggcgtgcgcc ggcgcccgtc
     4741 gccgagagct tggcggcagg tggggccgaa gcgccgcccg acatcagcaa catgtcgccg
     4801 cgggagcggt tcgaccggct gtacgatcgg gtgatgcggg ccgccagctc gggggacgag
     4861 gccaccgtcc agcggttcgg gccgatggcc gtcgccgcgt acgggatgct cgacagcgtc
     4921 gacgacgacg cccgctacga cgccgcgctc ctcaagatcc atctgggcga cgtggccggt
     4981 gccaaagccc tggccgatac cattctccag cgtacgccga cccacctctt tggcctgatg
     5041 atccggggca cactcgcgcg cgccgccaag gacaccgtcg gcgcccggcg gaacgagcgc
     5101 gccttcctcg cggcgtacaa caaggagatg gccaaggccc ggcccgagta tgccgagcac
     5161 aagtccgccc tcgacgcatt ccgtgcccag gcccagggcg acgtgaaata gcgccgggct
     5221 tccccttggg gcgtgccgtg tatattggaa tccggaactg aattcaggac cggagccaag
     5281 ggagggcccc atgaaggcag gcacgaagtt cctgctcggc gcagcggtga tcgtcgccag
     5341 cgtgagcgtg ctgatggtgg aaggggtgaa ggagacgggg acctacttcc tgaccccgac
     5401 cgaattgaag ggcaaggtcg ccgccgaccc gacgttcgtg aacgtcggcc tcaaggtcgg
     5461 cgccaaggtg gtcaagggtt cccttacccg caacacggcc gcgcacgaga tcgacttcaa
     5521 ggtctccgac ggcaccacgg tctacccggt cacgtatcgc ggcaccatcc ctgacacctt
     5581 caccgacgcg gacgacatcg acgtcgtggt cgaaggccgc ctgcaggccg atggcgtgat
     5641 ccacgccacc gacgtcctgg ccaagtgcgg ttcccgctac gacgctgtgc ccaaggcctg
     5701 agccgaccgt gaccctcctc ggcacgttct gcctctgggc caccatcctg gtgagcacgt
     5761 ggggggcggt cctcgcgttc agcgggcgct ggcgcgggcg gccggagctg gtcggcagca
     5821 tccgtggcgc ggtgttcgcg gggttcggcc tcctggtggt ggccgcggcc tgcctctggc
     5881 aggtgctcgc cgcgcacgac ttcaacgtcg agtacgtcgc cgcctacacc tcgcgcaacc
     5941 tcccgaccta cttcctcgtc tccgcgttct gggccggcca gaagggctcg ctcctcctgt
     6001 gggccatcgt gctgtcgctc ttcgcggcgc tggcccagtg gctcaccgcc aagcgttacg
     6061 ccgacctgct cccgtacgtg gccggcgtca cctgcacggt gctcgtcttc ttcgtgctgg
     6121 tgatggtcac cagcgccaac ccgttcgagc gcctggccta caccccggcc gacggccgcg
     6181 ggctcaatcc gcagctccag catccggcga tgatcatcca tccgccgatg ctctacctgg
     6241 gctacatcag cgcagtcatc ccgttcgcgt tcgccatggc ggcgctcctc tcccgccggc
     6301 tggacacggg gtggatccac gccatccgca agtggaccct ggtctcgtgg ctcttcctgt
     6361 cggtgggcat cacgctcggg atgtggtggg cctacaccga actggggtgg ggtggctact
     6421 ggtcgtggga cccggtcgag aacgcgagct tcctcccctg gctcacgatg accgccttcc
     6481 tccactcggt gatgatccag gaaaagcgcg ggatgctcaa gcgctggaac ctgagcctca
     6541 tcatcgggac gttcctgctg accatcttcg ggaccttcct gacccgctcg ggcgtcatcg
     6601 ccagcgtgca cagcttcacc cagtcggcgg tggggtactg gttcctcggg ttcctgctgc
     6661 tggccggggt ggcctcgtac accctgctct acacccgctg gccccagctc gaggcggagg
     6721 tgcagctgga gtcgatggcc agccgtgagg cggcattcct cttcaacaac ctgctgctcg
     6781 tcgggatcgc gttctcggtg ctctggggca cgctcttccc gatcctctcg gagctggtgc
     6841 ggggcaccaa gatcaccgtg ggtccgccct tcttcaaccg ggtcaacggc ccgctcgggc
     6901 tcctgctcct ggcgctcacc ggcatcgggc cgctcatcgc ctggcgccgg gcatccaccg
     6961 ccaacctccg gcgacagttc gtgtggccgc tcgcggcctt cggcgccacc ttcgccgggc
     7021 tcctgctggc cggcgtccgc cagctctcgc cgctcatggc ctggtcgctc gccgggttcg
     7081 tcctcggcac ggtggtgcag gagttcgccc gcggcgtccg ggcccggcat cggatgtacc
     7141 acgaatcgcc ggtcgtggca ttcgtgcacc tggtcgcccg caaccggcgg cgctacggcg
     7201 gctacctggt ccacgtggcc atcctcatct acttcgtggc cttcgcgggc acggcgttcc
     7261 gcgaggacct gcaggtgtcg ctcaagccgg ggcagtcggt cacgatgcgg agcccgttcg
     7321 ggcacaacta caagttcacg cacctcggca tcagccaata cgacgcgctc aaccgctccg
     7381 tgtcggccgc gacggtcgag gtgagcgagg acggccggcc gctcggcgtg ctcaagtccg
     7441 agaagcggca gtacgtcgac gggctcggac ggcccacctt caatccgtcg accgagatcg
     7501 cgctccgcag cacgccgcag gaggacctct acctcgtctt cgagagctcg gtggacggca
     7561 ccgaggaggc caactaccgg ttcaccatca cgccgctggt ctggtgggtc tggtacggcg
     7621 gactgatgct ggtcatcggc ggcctggtga cgatgtggcc ggcgtcgacc ggtggcggcg
     7681 cagggtccaa gtcgccggtc gagccgggct atgcggccgc gctggccgcg tcgggcgggg
     7741 gcgccgcatg agcgcgatgg atcgccgcgg cttcctgggc gtcctcgcgc tcggggccgt
     7801 cccgctggcc ctgcacgcgg cccagcagca gcagccgggg gacacgacgc ggggccagat
     7861 cccgcagggc gacgccgggt tcctgcccaa tccggcgctg gccggtgtct cccgtccgcc
     7921 ggtgaccgcc caggagaacg atcccctcat cgtcggcatc gagcaccggg tgcgctgtcc
     7981 ctgcccctgc gggctcgacg tgtacacctg ccgcaccacc gacttcacct gcagttactc
     8041 gccggagcgc caccgggaga tcatgggcct ggtgcagcag ggaatgacgc ctgaccaggt
     8101 cgtggccacg ttggtggcca agtatggcga gtcgacgctg atggcgccca agccccgcgg
     8161 gttcaacctc gtgggctacc tcctcccggg gtcgctggtg ctcctgcttg gcgcggcgct
     8221 cacgtcggtg atcctctccc gccggcgcgg cgccgccccg gcggcagggt tcgtccccgc
     8281 gatggccggc gatcgcacgg tcgacgcttc gcccgatgag ctggcgaagc tcgcccaggc
     8341 catgcgggac ctggatcaat gaacgccctg gaggtgatcc tcggggtggt ggtcgccgcc
     8401 gccgcggtga ccctcgtcgc gtggccgctc tggcatccgc ctgcgccggg agtcgtcacg
     8461 ctcgatgacg cgccgtcgct cgaagagacc cgcaagggtg tggcgctgct ggcgctccgc
     8521 gagatcgagt tcgaccgcga gaccggcaag ctctccgacc aggattacca gcgcctcaag
     8581 tcggcgtata ccgccgaagc ggtgtctgca ctgcgggtgg aggaaggccg cgcggcccgc
     8641 gcttccgcgc ccgccgaagc actcatcgcc gagcgggtgc gggcgctcgg cgcccgggcc
     8701 ggcgctcagt cggcggccgg tgccggcccc agctgcagca cgtgcggccc ccggcccgaa
     8761 tccgacgcca tctactgctc gaattgcggg cagatgctcg tcggccatgg ctgcggccgc
     8821 tgcggcgccg cccttccgcc gggggccagg ttctgcgagt cgtgcggatc gccggtcgct
     8881 ggcgccgcct gacccgaagc cgttgatgct gtgaaatgcg cgggcccccg ggcgacgtct
     8941 cgcccggggg tccgctcatt tcaaggtgtg ccgcttcgct tggcttccac tgttccgctc
     9001 gcactgcacc tcgactgccg gccgccgcgc ccttggctca cggcgggcga ccgccctgct
     9061 gttcgcgctg tcgacctgtg cgtaccgctc ggtcccgcca ctcagagccg gtactccgcg
     9121 ccgatcccca cacgggtgcg ccacatcgtg tgctggctca ggtcgggcgt gaggtcggtg
     9181 tcggccagtt gggagcccgt gatcgcggcg tcgccccgga gcgaaagccc caggcgttcg
     9241 gtgatcggaa gctccagtga ggcaccggcg tgggcgcccg ctcgggccac tgaactcccg
     9301 ccgttgagct tccagaggtc gaggagcggg ccggcgtgga tgatcagccg gcccccggcc
     9361 gccgtcgccg ccagggagtg cctgacttcc ggcgccagtt cgacgagcgt gaggtcgccg
     9421 ccctgggtga gcgcgattgt gccctgggaa tatccggtgg ccgagcccgc gatcgtcagc
     9481 gctaccccga agcgcgcgcc tgacaggcgg agcgtcatca gggtcggtcg tgacgggcgg
     9541 atgtagaccg gtgtcgccga cgtgtcgcgg acgccgccgt ggaatcgggc ggcgccgaat
     9601 tcgagggtca gtcgggtgcc ggcaccctgc tgtgccacaa gggtggccgg ggccagggtg
     9661 atcagggcgc cgcagaggag cagtcgtcgc atcgaaggtc tccagggtcg gggccttccg
     9721 ggaggcgcgc gcgcctcccg gatggccggt gtcacttcat gtcgagccgg atccagccct
     9781 gtcgggcctg gtcgtagccg atgaacgacc agttggcgac cggatgggtg tagccggtga
     9841 tgctgttggc gatcgccacg ccgatgaggt cgtcgttgga cttgatccag ttgatggcct
     9901 ccgcgatgag cgcccacagg gccttgaccg cactgatcgt ggcgccgttg aggccgatgt
     9961 tgtccttggc ccccttgtac tggctcgtga agttgttcac ggctccgacg aatgccgcca
    10021 tgttggtgtt gtccaccttg acctggcagg cggtgtcgtc gtcctccagg gccaggatcg
    10081 agaaattctg gtccgggtat ttcgtgtgat actcgttgag ctgggtctgg ctgaagagca
    10141 tgaggtcgcc gctccaggtg gtgctgttga ggtcccagta tgtgttgccg tgctcgccga
    10201 cgcaccacag cgtgtggtag gtcgagttgt cgccgggggc gttggggccc agaatgtgga
    10261 gctcaaattc gggatccccc ttcaaccaac cctcaaacgt gcccgagaac tgcacatagc
    10321 tcatgtagag cccgtggatc atgacggtgc cgccgccggt gccgctcccc gacgtggtgc
    10381 ctgtcgtgcc cccgcccgat gagccgcaca cttccgtcgt gcaggtggtg agctcgggtg
    10441 ccgcgccggg atgatccagg tccgtttcct ggggctccac cgccaggacc ggcacggcgg
    10501 gtggccggtc aggggaaagg aggtggcggc ggcccttggt gtcgtaggcc accggggtgt
    10561 cgtggtcgtt gatggccgtg gccacgagga tgttctcatc gccctgccac gcctcccgct
    10621 gcccgtggac cgggaagtag aactcgaggg ggcgggcatc ggcggcgtcc gccgcgatgc
    10681 tcgtctgggg ctcgccgctg gcggcggcca tcgcccgcat gggagcggtg ccggggcggc
    10741 cgaggaaccg ctggaactgc agcttgtgct cccggaacgg cgaggcgccg agttccgacc
    10801 ggaccagcga ccggaaggac gggtcgccca gcgccagggc cgtgcgcttg gccaggcgct
    10861 cgaggcggcc ctgttcgcca tcggtcgggg cgggctcggt gacgacgacg tcggtgcccg
    10921 gtccgctggc gcccgtcggg ccccggtcat cggcgcaggc cgacgcgacg cagagcagcg
    10981 ccggcaccag ccagagcgtg gaataccgca tgtctcctcc ttgggtaggg gcgccaacgc
    11041 gcccgtgact gaagcgcgcc caaggtagga agcggtgtcc ggcggcccgt caccccgccg
    11101 ccgcggcacg gcacgaaaaa acgggacggc cgccccggcc gccccgctgg atgaccggtc
    11161 gccctcggtc agtggaacgt gagatcgatc ccgacggcga tcacgtcgaa cctggccccg
    11221 gtgatcatgg tcgatccgcc ctgcttgatg tcgccgggcc ggccggcgaa ccaggtgacc
    11281 tcaggggcga tggtcacgtg gcggccgagc gggaggtcgt acgccacgcc cgcgagcccg
    11341 ccgaatccga agccgctggc cgggacgaag ctccccggct ccaccgacat cccgctcgcc
    11401 ccggcgccga gcttgagccg gaggccgccc gtcccgggcg ggtagtagag cacgacggcg
    11461 gtgagggtgc cgtcgtactg gtcgcccacg ccctgcagcg tcctggacca cgccgtgagg
    11521 tcgaggccga tccggaggtc cggggtgagc gtcgttcccg cggccaggtg gacgacggtc
    11581 ccggtctggc gccccgatgc ggcgcacccg tcgcactgtt cggcttcgcg acccgcgccg
    11641 agtccggtag cgaaccagaa cccctgccgc ggccgcgatt cgtccgtgcc ggggcgctgg
    11701 gcggagaggg ggaacgccgc tgcgaggagc agggccatca gggtgacgtc gtagcgtgcg
    11761 cgcatcgcgc ctccgttggg gtcggttccg gaccccgtga ggtggcaatc ggcgtgccga
    11821 ggatggaaag tggcggagcg aagggagtgg atacgaggtg acgccgggcg gcggcgcgtg
    11881 cggcggggga gcgtcgcgcg tgtggcgcgg ggccgtcagt cgatctcggt gtcgaactgg
    11941 accagggtac ggagcgccac ccggagcgcc gtccacgcaa cggcaaggca cccggcgacg
    12001 accactccgg tcgcgatgat gatacccgcg acgccggggt gcccgtcgag ctgcgtgtcc
    12061 cggaggaacc ccgcgaccgg tccggcctcg atgacgatga ccagcaccag gagtccggtg
    12121 atggtcatca tgcacacgag tccgccgaaa ctggtcggga tctgtgccgc gttctcgctg
    12181 tcgaactggg gatacaccgc cccgaacgcc agcgccagcg ccgcgatggc aaaggtgagc
    12241 gcggccacgg tgccgacgtt catcagcagg atgaccggcg ccgacttgag cgacacgttg
    12301 gtgatgacgg tgaggccgag cgacaggacg aggagcggcg ccgtgccggt ccagtacttg
    12361 ctccggagca gtgccctcag gtcgagcggg ctcgaccgca agagccagaa ctgccgaccc
    12421 tcgagcgaca ccgccgggaa gatgaaccgc accgcgatgg ccgcgagcac gaacccgctg
    12481 agcccctggt tgaggaagat gaccagcgtg gtcacgaaga gcggcacccg ctccccggtg
    12541 ttgatgggca gggcggagat gttgaagagg tataccacca gcagcacgcc gatgaggatc
    12601 agctggcccc attgggtgga atcccggaag aagacccgca cgtccttgag gagcatctcc
    12661 cgctgcatcg cgcccagggg ccgcagcagc gccgacaccc agcgagcctg gcgccgcccg
    12721 gcgtcccgcg tatcgcctcc ctcctgcgcc cgggagaacc cggacgcata gagccggtgg
    12781 tggaggagtg cgcccagcac gacgaacgcc ccggcggtgc tccagaggag gaggatgggg
    12841 agcggatcgg ccacccggtc gagccagttc atcacggtgt ccgcggccca ctggctcggc
    12901 agcagcgggc tggtcggcgc ctgcaacagg gtcaggaagt cgatcaggtt ccggaatccc
    12961 tccggccggg cgagctgctc gggcctggcg agccggagcc agagcacgaa gccggcggcc
    13021 gccgccaccg ccagcagggt caggaggtcg cggagccgcc gggcggggaa gacgttcacc
    13081 aggagcaggg tcagcacggc gccgaccgcc gccgggatca cgaggagggg gaagagcgcg
    13141 gccgcggcca cgagcgggaa gagcgggccc ccgccgaaca cggcgccgta cgccgcgaag
    13201 atcggcacgg ccatcaccac caccatccac gacgaatgca ggagattctc cgccagcttg
    13261 gcgaggtaga accagagcca gtcgaccggc gcggagatga gcaggtcgag gtcgcgggcc
    13321 aggaagaacg acgacagcgc cgtgatcagg ttcgagagca gcaggagcga caagaacgcg
    13381 aggaacccca ctgccaggat cttgctggcg atgagcgggc cgacctccgg cgtgtccttg
    13441 aggtaccgga gcacccggtg cagcaggccg tacgtcccac cccagaacac cgtccccacg
    13501 acgagcagga ggaggacctt ggtgccggcg cccttgcgct cctggcgcag gcgcgcccgc
    13561 gcgctctgcc acttgggccg caggagatcg ggcatccacg gttcccgccc acccgccgtg
    13621 acctcgacgg cgctcacttg ccgaacaccg cctcgagcgc cccgacctca ccggtgcccg
    13681 tcagcttgag gaacacctcc tgcagcgaca tcccgtggga cgccgtctgt tcccggagtt
    13741 cgtgcatggt gcccacgcct gcgatccggc cgtggtggat gatcgcgatc cggtcgcaca
    13801 cggcctcggc cacctcgagg gtgtgggtgc tcatcagcac cgtgcccccg cggtccacga
    13861 agcggcggaa gaggtccttg aggaacgcct ggcccttggg gtcgagcccc accatcggct
    13921 cgtccaccac gatcacctcg gggcggtgga tgagggcgct cgagatgatg agcttctggc
    13981 gcatcccgtg actgtacttc tcgaccagct ggtccttcca gtcggtgagc tcgaagaggt
    14041 ccaggagctc cgccatccgc gcctccacgt ccgccccttc ctgccggtag agccctgcca
    14101 cgaagcggag gaactccgcc ccggtcagct tgtcatacac gtaggggcgg tcggggatga
    14161 agccgagttt ctgcttggcc gccaccggtt cgatcgcgat gtcgtggccg gcgatggtca
    14221 ccgtgccggt ggtgggctgc aggatcccgg cgatcatccg aagcgtcgtc gtcttgccgg
    14281 ccccgttcgg gcccaggaag ccgaagagct cgccgcgcgg cacgtggagc gagatgtggt
    14341 cgaccgcggt gaacgaaccg tagcgcttgg tcaggtcggt gaggcggatc agtcgccgtc
    14401 caggaacgcg gtggggcgca gggcgccggc gagcgggagg tactcggccc agtgggaggt
    14461 ggccccgacg accaggcggg cgtgggccgg tgaggcagga aattgcccga aggcggggtc
    14521 gaccggcacc cagtcgccga tgaaggcctc ggcccaggtg tggaggcgca cacctccccg
    14581 tccgatgagg agcccggcca ccgggcgcgc cggcagtccc acccgccggg ccagcgcggt
    14641 gaagaggatc gccagccccg ccgcgttccc gcgccggcgc gccagcacct gggcggcggt
    14701 aggccagggc ccgccggcgc tgtcgtcgac gagcgcgata ttccggtgga tccaccggac
    14761 caggctcgcg gtcgcgaacc gcgcgctcgt ctcggtgccg accacgctcc gggccagcgc
    14821 agtgacggcg gcgtcgtccg gcgagaggcg cggctcgccg cggagcacgc cgacgaaacg
    14881 gaggtcggtc atcgggatcg gctgcagcgg ccgcatcggg tcgcgcagcg gagcgaacgg
    14941 catccgggtg agcgccagcg tgtcgcccgc gaacgactgc atcggcccgg cgccctgcag
    15001 gtcggaatac tcggccccgg gaagcaccac gcgccacccg atgttccctg cggccagagc
    15061 cgagtcggcc ggacccgcgg tggcggggag ggagggcaat ccgatgggcg ggtgcgccga
    15121 atcgctcagg gccttgagcc cgtcattggt gaactcgaac gcggtgcgtt cgaaggtgag
    15181 gccgccgccg gtccagctcc ggaccggaaa gccgttgatg tcgatccagg cgtgggtcgg
    15241 cacgccgtgc tggaccatcg ccacccgcca cgcccgcagc gtatcggtgc gggccacccg
    15301 gtagccggta tcgaccttga tcacgctgtc ggggatcacg aatgtcgagt cgccggtgac
    15361 ggtggcgccg gccgcgtagt actcgagcgt ggccggatcg aacaccgggt gactcacggt
    15421 gacgccgatg ctgggtcgtt cccggtaggc cagctggatg gggaacgctc cgagcggcag
    15481 gagcggcccg tcgatccgct gcagcgaccg ccacgggccc ccggtcgagt cgagggtgcc
    15541 cgccagcagg gagtcaccgt acgtcacgac ggtggccacc gccttgccct cgggcccgga
    15601 gacctgaatg cccgtccggc gaaggccgag ccgggtggtg aggaaggtgt tgctggagaa
    15661 ttcgtgccgc tccgggccgt ggggtccggc gagcacgatg accagccgct ccgagagccg
    15721 cacctccgcc ggcgtggagt ccgtgggtga gagcgtgtcg acggtgactg acgcgagccc
    15781 gacctgccgt gccccccacg agacccggta gtacgccgcc cccggcggta cccgctggat
    15841 cgacgccacc gatcgccctc ccggcgcgcc atagtgccgt tccgcgagcc aggccagcga
    15901 cgcgacccac gccaccagga tcaccgccgc cacgaatccc cggctcacgg cgcccgacgc
    15961 ccgccgaggg ccgcttcgat ctcggcgcga tccccttcgc gctccaccat caccaccacc
    16021 gatgcggggt actcggcggg gcggagcgga tcgtcgaggg tgtctcggag gagccgcgcc
    16081 gcctcgtcga tggacagctg ccccgcgccg gcaccgacca gcggcatcgc gatggacacg
    16141 agctgccact gggccacccg ttgccacgcc gagacgaggg ctcgccgcac ggtgtcggcg
    16201 gtcgccggct gctcggccga ctggatgacc gcgtggacca cgaacttcgc ggggaggtcg
    16261 ccggcgccgg tcaccaccgc ggcccccggc accagcgcct gctgtgttcg ccgctgttcg
    16321 ctgaagcggg ggcccgcctg ccggtcgaga cgcgcggccg cggtcgtcac cggatcgagc
    16381 gccgcgtccg ccggccggat gaccgcatcg acctccgcga acgccaggtc atccacccgg
    16441 acctcgatca ccgggcggcc tcccgggccc gccggtacat ctcccgggcc tcctcgctcc
    16501 ggccctgtcg gtcgaggata cggccgagga gatggtaggc gcgcccctgc cggaggtcga
    16561 gctcggcggc gcggcggagg gccgcctccg ccccggccag gtcgccctgg tggtgcagcg
    16621 cttcgccgag atagaattgc gccggcgcat tggccggatc gacgtggatc gcgtgctgca
    16681 gcgtctcgac cgcctcggcc ggcaggccct tgcgatggag caggaggccc agctggaaga
    16741 gcgatgcggg atgttccggc gactcccgca gggcgcggcg gagatctgct tccgcctcgg
    16801 cgtagcgctt gaggaggccc agcacggcgc cccgctggca gagcaggccg gggtccgtgg
    16861 ggcggtccgc cagcgccagg gtcagctgat cgaccgcgcc gtccaggtcc tcctgcgcct
    16921 ccaggatcgc cgcgagcgcc gcgcgcgcct cgacgtgatg ttcgtcgagc tccaggaggg
    16981 atcggtaggt gatcaccgcg tccgacggcc gctggcgcgc cacctgctcg gcggcccgcc
    17041 tgaagagcag gtcgctctcc gagccctcgg ccaacggatc ggtgaccacg atgtcatcgc
    17101 tgggaagacc ggcggcgagc ggacggagcg ggaaacggac gtccgcgcgg gaggggtagg
    17161 gctcgccctc catgccgtcg ggggtgaaga ggtcgtggag cgccgggtcg cgatcggcgc
    17221 tgcggctctg gcggaagcgg cgcatggatg ggatgtatgg taatcgggct atcagctatc
    17281 agctatcagc gtcgctggga gatcccgccg tcccgtgcca cgcgccaccc gcattcacca
    17341 ccggtgaccc gccactcgcc acgcgccacc ccttgaatgc cccccaccat cccgggtacc
    17401 ctacaaggct atggctcagg actccctcgt cgtacgcggc gcccgcgaac acaacctcca
    17461 gaacgtctcg gtcacgatcc cccgggacaa gctgacggtc atcaccgggc tttcggggtc
    17521 gggaaagtcc tcgctcgcct tcgatacgat ctacgctgag ggccagcgcc ggtacgtgga
    17581 gtcgctctcc gcgtacgccc gccagttcct cgggctgatg gaaaagcccg acgtcgacgc
    17641 gatcgagggc ctgtcgccgg ccatctcgat cgagcagaag tcggcgtcgc agaacccgcg
    17701 ttcgaccgtc ggcaccgtca ccgagatcta cgactacctg cggctcctct gggcccggac
    17761 cggcgttccg cactgtccca acgacggcac gccgatcacc cggcagagcg cctcccagat
    17821 caccgacacc atcctcggct ggcccgaggg gacgcggatc gaggtcctgg cgccgctggt
    17881 ccggggccgg aagggggagt tccgcgagct cttcgaggat gcacgccggc aggggtttgc
    17941 acgcgtccgg gtcaacggca ccacctacga gctcgaccag gtgccggcgc tggaacggcg
    18001 caagaaccac gacatcgccg tcgtggtgga ccggctcgtc gtccggcagt ccgaccgcgg
    18061 ccggctcaat gattcggtcg agactgcgct ccgcgccgcc gacggtgtgg tggaggtcgt
    18121 ggagcatggt ctgcgatccg cggtgagcgg tgaaaagggg gcgggctctg cgacccgtga
    18181 cccgcgaccc acgaccaagt cccacatctt ctcggaaaag ttcggctgcc ccgtctgcgg
    18241 attgtcgctg ccggaactcg agccgcgcca gttctcgttc aattcgccgt tcggcgcctg
    18301 ccccgactgc cacggtgtcg gcacccgacg cgaggtgaac gccgacctgg tcctgggcga
    18361 cgcgagcatc tccatcctcg aaggtgtcat cctcccctgg ggcgagccga gcggctacct
    18421 caggaaggtc gtgctcccca ccctggccaa ggcgttcaag ttcgacctca acgccccgtg
    18481 gcgcgaccac agcgcggcgg ccaagcacgc catcctccac ggtgaaccgg ggcgggtgct
    18541 caagttcgcc gcggaggggt ccaagaaggc cgaatacgaa agcgactggg agggcgtcct
    18601 ccggaatatc gagcgccggt accgcgagac caccagcgac tcgatccgcg tcgagctcga
    18661 ggacttcatg gtggagcagc cgtgccagac ctgccacgga cggcggctca agccggaaag
    18721 cctctcggtc accgtgggcg ggaaggggat cggcgaggtg gtcgagatgc cggtcgacca
    18781 ggccctcggg ttcttcggcg gcctgggcgc aaaggcggac gggaagcgtg ataaaggaaa
    18841 ggggaacggc gggctcgacg cggaggtgtc cgggccgatc ctcaaggagg tcgtcgaccg
    18901 gctccgcttt ctccgcgacg tggggctcgc gtacctgacc ctcgggcggg gggcggcgtc
    18961 gctttcgggc ggcgaggtcc agcgcatccg cctggccacc cagatcggct cgcggctcgt
    19021 gggcgtcctt tacatcctgg atgagccaag catcgggctg caccagcggg acaacgagcg
    19081 gctcctctcc acgctgcgcg agctccgcga cctcggcaac accgtcatcg ttgtcgaaca
    19141 cgacgaggac accatccgtg ccgccgacca cgtgatcgac ctgggccccg gggccgggaa
    19201 gctcggcggc aaggtcgtgg ccgaaggcac cgtccaggag atcctccggc atccgacctc
    19261 gctcacggcg cgctatctcc ggcgcgagct ccgtgtccgc gtgcccccca agcggcgccc
    19321 cggcagcggc gagcggctca agatcatcgg cgcctcgggg aacaacctca aggacctgac
    19381 ggtcgacgtg ccgctcggca agttcgtggc cgtcaccggc gtgtccggat cggggaagtc
    19441 gacgcttgtc accgacatcc tgtaccaggc gctggcgcgg catttctatc gtgccaaggt
    19501 ggtgccgggg cagcacaagc gcatcgaagg gctcgacaag atcgacaagg tcatcgacat
    19561 cgaccagagc cccatcggcc ggacgccgcg ctccaacccg gccacctaca ccgggctctt
    19621 caccccgatc cgcgaggtct tcacccagct gcccgaggcc aagatccggg gctacggccc
    19681 cggacgcttc tcgttcaacg tcaagggcgg gcggtgcgag gcctgccagg gcgatggcct
    19741 cgtcaagatc gagatgcact tcctgccgga cgtctacgtg ccgtgcgagg tgtgcaaggg
    19801 ccggcgctac aatcgcgaga ccctcgaggt gcgcttcaag ggccggagca tcgccgacgt
    19861 gctcgacctc accgtggctg atgcgcggga gttcttcgag gcgcagcccc gcatcgccga
    19921 gaagctcgaa ctcctgaacg atgtgggact gggctacatc cacctgggcc aggcggccac
    19981 tacgctctcc ggcggcgagg cgcagcgggt caagctcgcc accgaactct cgaagcgcga
    20041 caccggccgg accctctaca tccttgacga accgaccaca ggactccact tcgaggacgt
    20101 ccggctcctg ctcgaggtgc tgcatcggct ggtggacaag gggaacagcg tcgtcgtcat
    20161 cgagcacaat ctcgacgtcc tcaagaccgc cgactggatc atcgacctcg gcccggaagg
    20221 gggcgaaggc ggcggccgga tcgtggccga agggacgccg gagcaggtgg ccgccaccaa
    20281 ggggagcttc accggcgagt tcctcaagcg cacgctcgcg gaggcgtagg cctcgctccc
    20341 gacttccggt ccgggccggt accttaccgg tgtccctcgt cccggaggcc ggcatgaagt
    20401 accaagacat cctcgacctg ctcggctacg ccaacggtca cgacgtcgcg gtacgggtca
    20461 ccaccagcga cggcaccgag gtcgtgggga tcccgacttc ggtcgatacc catgtcaccg
    20521 cccacgaggt ctatctcagg ccggtgggca atgacgagac cgagatcggg ctgtcgctgg
    20581 gggagatcgt cgcggtggag ctggtgtagc gagggggtgg gtcagtgggg cagtggctca
    20641 gtgggtcatt tgttggtgcg ggcgtcctgg atcgatcgca gaaggccata tccgcagcga
    20701 cttgcctcct ccgcaagcac atggatccgc tgatattcgt cgtccgccaa ccagccgaga
    20761 tccttggcgg cccggagtga gtatttcaat tcggccaacg agccgagtgc gatgctgacg
    20821 tgccgcttga actccggagc accaaaccgg gcctgcccct ccgcgatatt ggccggtacc
    20881 gagaacgcgg cccgcctggc ctgactcacc attccgtaaa gttcggatct gggccaaccg
    20941 gcggtgattc gatacactgc gaggaagagg gtgtgcgcct tctgccatgc gaccagcgat
    21001 tcgaaaggct ccacggtacc cgtcgggaaa tggcacccga acatggattg gcgccctcaa
    21061 tccagcatca accaccccac cggctcactg acccaccctc ccaccgaccc actatctttc
    21121 ctttatggtc tccctcctcg acatcatcgg cccggtcatg gtcggcccct cctcgtccca
    21181 tacggcgggg gcgtgccgga tcgggctcat cgcgcgcggg atggtgggcg gcacacctga
    21241 agcggccaag gtcgagctgc acgggtcgtt cgcccgtacc ggcgtcggcc acggcaccga
    21301 tcgtgccatc gccggcgggc tgatgggctt cgccccggac gacgagcggc tccgcgagtc
    21361 actggagctc tccgagaagg cgggactcgc gatcgagttc gccaacgtca agctccgcgg
    21421 cgaccaccat cccaacacca cccgcatcgc cctcacccgc ggagccgtca cggccaccgt
    21481 catcgggtcc tcgctcgggg ccggccggat cctcatcacc gagatcgacg gctacccggt
    21541 cgagatcacc ggcagttatc cggtgctcgt catcgtcgcg cacgacgaac ccgggatcgt
    21601 cgccaaggtg tcgggcgtgc tcgccgcgga gcagaacaac a
//
LOCUS       DASUAP010000201         7220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1137024, whole genome shotgun sequence.
ACCESSION   DASUAP010000201 DASUAP010000000
VERSION     DASUAP010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137024"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..604)
                     /gene="rnpB"
                     /locus_tag="VFU45_05140"
     ncRNA           complement(207..604)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFU45_05140"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(773..3874)
                     /locus_tag="VFU45_05145"
     CDS             complement(773..3874)
                     /locus_tag="VFU45_05145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5358479.1"
                     /translation="MTVDITELERRLQSALGAQYRLEGELGQGGMGVVFLARDVTLDR
                     PVAVKVIRPELAIHSSITQRFLAEARLVARLRHPNIVTVHTAGETAGLFYYVMDLVPG
                     ESLRQRLARVGKLPADEVTRIVADLADALAAAGRQGVVHRDMKPENVLLDRDTGRALL
                     TDFGIARAMVAEGSNVITGQGMAVGTPTYMSPEQAAGEDVDHRSDIYALGIVAYEMLA
                     GYPPFRGASNAAVVAMQIAEAPVPIQKLRPDTPPALAGAVMKALAKDPAARWSDGLTF
                     RRAVLGEVPLAARGRRWLPAAVAAALLAGAGLLFALRRGGPPRGVDPRHSILVLPFDN
                     LRRDSTLEWLRQGSVSMLALDLAQWNDLTVVDQERVHDLYAKHQLAPDDPIGLDMARQ
                     LARDAGAWTLVLGDYERTGDSMQVTARMLDVASGKRVDLAQAAVAADSDVRPAFDQLA
                     ARLLDVSGAPGGLKPGLAGATTSSLEAYRAYLTGIDELNHWNLESADTALRRAIALDS
                     TFGLAYYKLALTRGWIGGANDQDGRRALVYASRYAGRLPARQQSLISAYQSFLDGDLP
                     HARELYAKLVARDSIDTDAWYALGDAWFHDPDSTAAGRAKKMTNSLRAFRRALALDPA
                     YALAYEHVAMMYTDAARDRPFFALVTNDSFAPGATLDSAVRSQAIERARRAGIQLSRD
                     WVAAQPGTERAHRALIDAYAAAHDVPDALQEIDRLRLPPGETDAVTATLLEGRARFAA
                     GDISGAIATLRPAYDSLDDDPARIAQMSATDYQMLLATVNPFLYAGDLGTATRVIALT
                     RGARQRMPPEGRDTSVDWRYYDWVQEGQYYGPAGSSTELRKAWRTVAEAARNAKPQGR
                     AAIARAGAAAALGLLLSPEGDTIPVTELRALTGDPLPGDVAALVAIARGDTSAARQSL
                     ATTAPAPMGTYRWGLGGWVGTPRLLTAYAHFQLGDYSRTLDLLSDYTPEDLQPNALDP
                     RWAALGWARLVRGLADERLGRKIDAEREYRSVLAQWKDADPKQVPLVLQARAGLGRVT
                     GTG"
     gene            4104..4961
                     /locus_tag="VFU45_05150"
     CDS             4104..4961
                     /locus_tag="VFU45_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5358480.1"
                     /translation="MTTLGNDHGAVAAVLHPRERAQVEAAMGGAVSVVHRDSIPEAIR
                     VVRERPLRAVLVSVHRCSRESLGDVDQLVRGFPGVPTVALVSEHGTGTTETLLHFGAR
                     GVREVVDVTDPGGWQRLRALVAGPAPLAGSRIQASILEILADAPADTRLFFEVLVRIA
                     PTITTVRKLCTALKVRPSTLMSRFGRAGLPSPKSYLAGMRLLHASLMLEDAGLSVADV
                     AYRLDYSSPQSFGRHLRALMGVTGTEFRRRFPFPVALRRYVDLMILPYRTVLCAFHPL
                     SSGAWDSGH"
     gene            complement(5205..6764)
                     /locus_tag="VFU45_05155"
     CDS             complement(5205..6764)
                     /locus_tag="VFU45_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5358481.1"
                     /translation="MADTADSTNLFHRTTRRRWWLIAGATVALLAAGPATGADIAWAP
                     VVELLALGGAVNLGAAILGRRRLTGRAAWAGSLVLDLIITAVPIALTGLGGLGVLPLV
                     ATLPYAADDPGITGALAALSGGLAVLLAQFVAGAGTLPAPALLEGALVALVGMALRER
                     AAMTEARFRRLADGLRRAGAGDFSTRLGAQARDTLGAVETEFDRMATATGAMAETVKA
                     ESALVAAVAEELSAATEELQLSADALSGAAGRLAQDLQSQRLMAEDSRRQGEGAATEA
                     ELQRERFRALEAEEARLVSVADLARERVARASDTLVSVGNEVTTTGRIVSELTAMSAR
                     IGTFAQTIAQIARQTHLLSLNAAIEAARAEGEGQGFSVVAEEVRALAAQAGRSAREVG
                     ELVGELQAGIAAAERAMRSGSSQVEDIARVAREADGALLELTSGVRRTAERVSAAATS
                     THAQAGHQTALRQVLDRVSEVSHGAASSSDAAARSAQQQIQAMTELSATSQQLALLAE
                     RLRSATAHLKA"
     gene            complement(6776..>7220)
                     /locus_tag="VFU45_05160"
     CDS             complement(6776..>7220)
                     /locus_tag="VFU45_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU5358482.1"
                     /translation="TTMSAEGQALIGRLGRAAIAAAGWRPRSVGGLTMGADPVAYAIA
                     RASVDTPPLIDAFSVRKAAKDHGTGRLIEGNFDAGDAVVVVEDVITSGGSALQAIRTL
                     EAAGARVLGVFAVVDREEGGRAAIEAAGHAVIALTTASALGLKSA"
BASE COUNT          907 a         2603 c         2665 g         1045 t
ORIGIN      
        1 gcccggtccc gggatgcgga gccggccgcg cgggggtcgc gggaacgacg gtcgctcgac
       61 acggtgagga ttctacggag gggggacagg gggggcaagt ttggaggggt cgttggtcgt
      121 cgggcttcgg gccggggaca ggtgccaacg acgaacgacc aatgcccccc caatggagtc
      181 ccccgccctc gtttccggga gcgggggaca cgggtcaagc cgtaagccga gttctgttcc
      241 cggcgccttg cggcgccggg gatggtcatc tgtctgggac ggcggtcgcc cgacgcctca
      301 agcagcctac ccgcggctca ccgttgccgg tgttgtcggt gcgggccgca cctcgccgct
      361 tatttggcct tgctccgacc gggggttacc gtgccgtccc tgttgccagg ggcgcggtgg
      421 gctcttaccc caccgtttca cccttgcctg tgtccctcgc ttgcgcgcgg gaccatcggc
      481 ggtctgctct ctgttgccct gtccgtcgcc tcgcggcgcc caggcgttac ctggcgatct
      541 gccctgtgga gctcggactt tcctcgacgg ggtctccccc gccgcgacca tcacgctcgg
      601 cccgtgctcg aaagatagca gggatccgag cccgatgcga aggagtcgtg ggtcgtgggt
      661 cgtgagtcgt gagttcatgg tcgatcggat cggctcggcg ggccagtgct gtccgaacgt
      721 cagcggatgc gaccatcgac ccacgaccca cggctcgcga ctcacgaccc cctcaccccg
      781 tccccgtcac ccgcccgagc cccgcccgcg cctgcagcac cagcggcacc tgcttcgggt
      841 cggcgtcctt ccactgcgcc agcaccgagc ggtactcccg ttccgcgtcg atcttgcggc
      901 cgagccgttc gtcggcgagc ccgcgcacca ggcgggccca gccgagcgcg gcccagcggg
      961 gatcgagcgc gttcggctgc aggtcctcgg gggtgtagtc gctgaggagg tcgagcgtcc
     1021 gggagtagtc gccgagctgg aaatgggcgt aggcggtgag gaggcgtggc gtgcccaccc
     1081 agccgccgag cccccagcga taggtgccca tgggggcggg cgcggtcgtc gccaggctct
     1141 ggcgcgcggc gctcgtgtcg ccgcgggcga tggccacgag cgccgccacg tcgccgggga
     1201 gcgggtcgcc ggtgagcgcc cgcagttcgg tgaccgggat cgtgtcgcct tcgggggaga
     1261 ggaggagccc gagcgccgcc gccgcgccgg cgcgcgcgat ggcggcgcgt ccctgcggct
     1321 tggcgttgcg ggccgcctcg gccacggtgc gccacgcctt gcgcagctcg gtgctcgagc
     1381 ccgcggggcc gtagtactgc ccttcctgca cccagtcgta gtagcgccag tccaccgacg
     1441 tgtcgcgccc ctcgggcggc atccgctggc gcgcgccgcg cgtgagggca atgacccgcg
     1501 tcgcagtgcc gagatcgccg gcgtagagga acgggttgac cgtggccagc agcatctggt
     1561 agtcggtggc ggacatctgc gcgatgcgcg ccgggtcatc gtccagggaa tcgtaggccg
     1621 gccgcagcgt cgcgatcgcc ccggagatgt cccccgccgc gaagcgcgcc cggccctcga
     1681 ggagggtcgc ggtcacggcg tcggtctccc ccggcggaag ccgcaggcgg tcgatctcct
     1741 ggagcgcgtc gggcacgtcg tgcgccgccg cgtaggcgtc gatgagcgcg cggtgcgccc
     1801 gctcggtgcc gggctgggcg gccacccagt cgcgggagag ctggatcccc gcgcgccggg
     1861 cgcgttcgat ggcctggctg cgcacggcgc tgtcgagggt ggcgccgggg gcaaaggagt
     1921 cgttggtgac gagcgcgaag aacgggcggt cgcgcgcggc gtcggtgtac atcatcgcca
     1981 cgtgctcgta cgccagcgca tacgcggggt cgagcgccag cgcccggcgg aacgcgcgga
     2041 gcgagttggt catcttcttc gcgcgccccg cggcggtcga gtcggggtcg tggaaccagg
     2101 catcgcccag ggcgtaccac gcgtcggtgt cgatgctgtc gcgcgccacc agtttcgcgt
     2161 agagctcgcg ggcgtgcggg aggtcgccgt cgaggaagct ctggtaggcg ctgatgagcg
     2221 actgctgccg ggccgggagg cgtccggcat agcgcgaggc atagacgagg gcccggcgcc
     2281 cgtcctggtc gttggcgccg ccgatccacc cacgggtgag cgcgagcttg tagtacgcca
     2341 ggccaaaagt gctgtcgagc gcgatcgcgc ggcggagcgc cgtgtcggcc gactccaggt
     2401 tccagtggtt gagctcgtcg atcccggtca ggtaggcgcg gtacgcctcg agcgaggagg
     2461 tcgtggcgcc ggcgagcccg ggcttgagcc cgcccggcgc gccggagaca tcgaggaggc
     2521 gcgcggcgag ctggtcgaag gcgggccgga cgtcgctgtc ggcggccacc gccgcctggg
     2581 ccaggtccac ccgcttcccg gaggccacgt cgagcatccg cgccgtgacc tgcatcgagt
     2641 cgccggtccg ttcgtagtcg cccaggacga gcgtccacgc tcccgcgtcc cgcgcgagct
     2701 gacgcgccat gtcgaggccg atcgggtcgt ccggggccag ctggtgcttg gcgtagaggt
     2761 cgtgcacccg ctcctggtcg accacggtga ggtcgttcca ctgggccagg tcgagcgcca
     2821 gcatgctgac actgccctgg cggagccatt cgagggtcga atcgcgccgc aggttgtcga
     2881 acggcagcac caggatggaa tgtcgcgggt cgacgccgcg gggcgggccg ccgcgccgga
     2941 gcgcaaagag aagtccggcc ccggccagga gcgccgccgc caccgccgcc gggagccagc
     3001 gccgcccgcg ggcggcgagc ggcacctcgc ccagcacggc ccggcggaag gtgagcccgt
     3061 cgctccaccg cgcggccgga tccttggcca gcgccttcat cacggcgccc gcgagcgccg
     3121 gcggcgtgtc gggccggagc ttctggatcg gcaccggcgc ctcggcgatc tgcatcgcga
     3181 ccaccgccgc gttactggcg ccgcggaacg gcggataccc ggccagcatc tcgtaagcca
     3241 cgatgccgag ggcgtagatg tcgctgcggt ggtcgacgtc ctcaccggcg gcctgctccg
     3301 ggctcatgta ggtcggcgtg ccgaccgcca tcccctgccc ggtgatgacg ttcgagccct
     3361 ccgccaccat cgcccgcgca atgccgaagt cggtcaggag cgcacggccc gtgtcccgat
     3421 cgaggagcac gttctccggc ttcatgtccc ggtgcaccac cccctgccgt ccggcggccg
     3481 cgagcgcatc ggccaggtcg gccacgatcc gcgtcacctc atccgccgga agcttgccga
     3541 ccctcgccag gcgctggcgc aggctctcgc ccggcaccag gtccatcaca taatagaaga
     3601 ggccggcggt ctccccggcg gtgtggacgg tgacgatgtt gggatggcgg agccgcgcca
     3661 ccagccgggc ctcggccagg aagcgctggg tgatcgacga gtggatggcc agttcggggc
     3721 ggatgacctt gacggcgacc gggcggtcga gcgtcacgtc gcgcgccagg aacacgacac
     3781 ccatcccccc ctggcccagc tccccttcga ggcggtactg ggcgcccagg gcggactgca
     3841 ggcggcgctc gagctcggtg atgtcgacgg tcacgcggtc ggcccccgct ccggtcggac
     3901 ggggccagta gggctccccg caagaactgt gaagatacgt cctggccgcc cgggggccag
     3961 agacgcacgt ccatgcaagg acgcccgaca gcccccaaag gttgcgttct gcacccccgg
     4021 gaacgaggcg gtacccccgg acgattgacc gccggttgag cccctcggcg taacctcccg
     4081 ccgacgatca accgggggac gatgtgacga cgctcggcaa cgaccacggg gcggtggccg
     4141 cggtgctcca tccgcgggag cgggcgcagg tggaggcggc catgggcggc gcggtgtcgg
     4201 tggtgcaccg cgactcgatc ccggaggcca tccgcgtcgt ccgggagcgg ccgctccgcg
     4261 cggtgctcgt ctcggtgcac cgctgctccc gcgaatccct cggcgacgtc gaccagctgg
     4321 tgcgcggctt tcccggcgtg ccgaccgtgg cgctcgtgtc ggagcacggg accggcacca
     4381 ccgagaccct ccttcatttc ggcgcccgcg gggtccggga ggtggtggac gtcaccgatc
     4441 ccggcggctg gcagcggctc cgcgcgctgg tggcgggacc cgcgccgctc gccggctccc
     4501 ggatccaggc ctcgatcctc gagatcctgg cggacgcccc cgccgacacc cgcctcttct
     4561 tcgaggtgct ggtgcgcatc gccccgacca tcaccaccgt gcggaagctc tgcacagcac
     4621 tcaaggtgcg gccgagcacg ctgatgtcac gcttcgggcg ggcggggctg ccgagcccca
     4681 agagctacct cgcggggatg cgcctgctgc acgcgtcgct gatgctcgag gatgcgggcc
     4741 tttccgtggc cgacgtcgcc taccggctcg actactcctc gccgcagagc ttcggccgcc
     4801 acctccgcgc actgatgggc gtcaccggca ccgagttccg gcggcggttc ccgttcccgg
     4861 tggcgctccg gcgctatgtc gacctgatga tcctcccgta ccggaccgtg ctctgtgcct
     4921 tccatcccct ctcttccggg gcctgggaca gcggacactg agcgtgcagg gccggcaccg
     4981 ggcgggagga tccagaaatg gaagagtgaa aagggaagag tgaagagtga aaaggggaaa
     5041 agtaagaagg gaaaagtgaa gagggtctgc ctttcgcccc ctgttccctt ttcccctttc
     5101 acttttccct tttcactctt ctctcttcac ttttccctct tcactcttct ctcttcactt
     5161 ttcccttttc actcttccct tttcactctt ctctgtcccg acgatcacgc cttcaggtgc
     5221 gccgttgcgg accggagccg ttcggccagg agggcaagct gctgggaggt ggccgagagc
     5281 tcggtcatcg cctggatctg ctgctgggcg gagcgggcgg cggcgtcgga gctgctggcg
     5341 gcgccgtggc tcacctcgct cacccggtcg agcacctggc ggagcgcggt ctggtggccc
     5401 gcctgggcgt gcgtgctcgt ggccgcggcg gacacccgct ccgccgtgcg ccggacgccg
     5461 gaggtgagct cgagcagcgc gccgtcggcc tcccgcgcca cccgcgcgat gtcctccacc
     5521 tgcgagctcc ccgagcgcat cgcccgttcg gcggcggcga tccccgcctg cagttcgccg
     5581 acgagctcgc ccacttcccg tgccgagcgc cccgcctgcg cggcgagggc gcggacctcc
     5641 tcggccacga cgctgaagcc ctgcccctcc ccttcggccc gcgccgcctc gatggcggcg
     5701 ttgagggaca gcaggtgggt ctggcgcgcg atctgggcga tggtctgcgc gaaggtgccg
     5761 atccgcgcgc tcatcgccgt gagttccgag acgatgcgcc cggtggtcgt cacctcgttg
     5821 cccaccgaca ccagggtgtc gctcgcccgc gcgacccgtt cgcgcgcgag gtcggcgacg
     5881 gagacgaggc gcgcctcctc cgcttcgagt gcgcggaagc gttcccgctg gagctccgcc
     5941 tcggtcgcgg caccctcgcc ctggcgccgg ctgtcctccg ccatcaggcg ctggctctgc
     6001 aggtcctgcg cgagccgccc cgcggcgccg gagagcgcgt cggccgagag ctgcagctcc
     6061 tccgtcgcgg ccgacagctc ttccgcgacc gcggccacca gggcggactc ggccttgacg
     6121 gtctcggcca tcgcgccggt ggccgtggcc atccggtcga attcggtctc cacggcgccg
     6181 agcgtgtcgc gcgcctgcgc gccgagccgg gtggaaaagt cgccggcgcc ggcgcgccgg
     6241 agcccgtcgg ccagtcggcg gaaccgcgcc tcggtcatgg cggcgcgctc acggagcgcc
     6301 atgcccacca gggcgacgag cgcgccctcg aggagcgccg gcgccgggag cgtgccggcg
     6361 ccggccacga actgtgccag cagcaccgcc agcccgccgg agagcgcggc gagcgcgccg
     6421 gtgatccccg gatcgtccgc ggcatacggg agggtggcca ccagcgggag gaccccgagg
     6481 ccgccaaggc cggtgagcgc gatcggcacc gcggtgatga tgaggtcgag aacgagggag
     6541 cccgcccacg cggcgcggcc ggtgagccgg cggcgcccga ggatcgcggc gccgaggttg
     6601 acggcgccgc cgagcgccag gagttcgacc accggtgccc acgcgatgtc ggcgccggtg
     6661 gccgggccgg cggcgaggag cgccacggtg gcgccggcga tgagccacca gcgccggcgc
     6721 gtcgtgcggt ggaacaggtt ggtggagtcg gcggtgtcgg ccatcggcgg cggggtcagg
     6781 cggacttgag gccgagggcc gatgccgtgg tgagcgcgat gacggcgtgg cccgccgcct
     6841 cgatcgcggc gcgccccccc tcttcccggt cgaccacggc gaagacgcca agcaccctgg
     6901 cgcccgcggc ctcgagggtg cggatggcct ggagcgccga gccgccggag gtgatgacgt
     6961 cctccacgac caccacggca tcgccggcgt cgaagttgcc ctcgatgagc cggccggtcc
     7021 cgtggtcctt ggcggccttg cgcacgctga acgcgtcgat caggggcggt gtgtcgacgc
     7081 tggcgcgcgc gatcgcgtag gccaccgggt cggcgcccat cgtgagtccg ccgaccgagc
     7141 gcggccgcca gccggccgcc gcgatcgcgg cgcggccgag ccggccgatg agcgcctggc
     7201 cctcggccga catcgtggtg
//
LOCUS       DASUAP010000202         4962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_400835, whole genome shotgun sequence.
ACCESSION   DASUAP010000202 DASUAP010000000
VERSION     DASUAP010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400835"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFU45_05165"
     CDS             complement(<1..276)
                     /locus_tag="VFU45_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358483.1"
                     /translation="MDGRDVAMDPSSLPTIAGARARAGRRPSLDLDDSRSIGERVFEW
                     LLVTVWLGVAVTTFAYGLPYYRLPLAARATSPMDELLRPSGLIGQGYG"
     gene            647..1102
                     /locus_tag="VFU45_05170"
     CDS             647..1102
                     /locus_tag="VFU45_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358484.1"
                     /translation="MKALATVIRRAPRSAAAIAFGACTATLAHFAWHADVRLDGTIPV
                     LTLGAGLAHAMAGVLIGRRLLDTARTPSAANAALLGAGASLIAQALFVPVLAAYVAES
                     GAAPTIIGYVGLTIYVAIFAFLAAGWAMLLLCMAVGWGLYKVAAPPAAG"
     gene            complement(1220..2905)
                     /locus_tag="VFU45_05175"
     CDS             complement(1220..2905)
                     /locus_tag="VFU45_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEU5358485.1"
                     /translation="MIRRALLTALAFALGAVALTAQDPGMAGMQMGADSADRMPIPMP
                     RGMIMLPGLVGLMPPVGAFLPGGGVDPATVPRALPSTIVPLNDGDTLDLTAGLLRRTI
                     RGRTFVMYGFNGQVPGPMIRVAQNATITVRFHNRIDLPSTVHWHGVRLDNRSDGVPGV
                     TQDPVAPGGDFTYTVHFPDAGIYWYHPHVREDIEQSMGLYGNMLVESPDTAYYSPANR
                     EVALMLSDLLIHADTLIPFGKEASDFVLMGRVGNVPLVNGEPDFQLTVHRGDVVRFFL
                     TDAANARTWNLSIPGVPLKVLASDVGRFEREAMVASVILAPAERYVVAAEFDRPGRYP
                     IVNAVQAIDHYRGEFETQVDTLGWATVDSTPAAPDYRAAFAALRANPAVTAEIGKYRQ
                     YFDRPPDKRITLTVATSSLPLPTVQFMSVDTAYFHPVEWTDGMPEMNWLSTANEVRWI
                     LRDDSTGAENGAIDWHVRQGSVVKLRIFNDPHSFHPMQHPIHLHGQRMLVVSRDGVRT
                     TDLVWKDTVLIPVGSTVDVLIDASNPGTWMLHCHIAEHLGSGMMMTLHVDPRR"
     gene            complement(2902..3384)
                     /locus_tag="VFU45_05180"
     CDS             complement(2902..3384)
                     /locus_tag="VFU45_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358486.1"
                     /translation="MRASAVLGCLALAVASAGPKPGPYDMALAPGSAVPSARGSARLV
                     FADSPFGVAATADGRLRYDIRVMASGLPAPATLGPYRAYVAWEVSPDLSDWRRLGVIA
                     NGVSTVGVAERNKFLFVITAEADPTPAAHAGPTVLHGTSPSGWLQQVLSHPLCRGAPG
                     "
     gene            complement(3381..4745)
                     /locus_tag="VFU45_05185"
     CDS             complement(3381..4745)
                     /locus_tag="VFU45_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358487.1"
                     /translation="MRGALFALALLGVAATASAQDPGAKVPTDHKIGGSKNIKVVAHV
                     VTHPGAWKLADVEIEQDMNRPYVYVCGFVNNDVQIYDIKDPSHPKKIVDWSIENPDLH
                     RGIGAMDGKAFKIGDRYYYAQSYQFMQGSPDADLGAVILDVTGLPDPSTVKVVARIRY
                     PAAPGGFHNTFVYKHSDGRVLYFVTVNQAKSVIYDLAKVVAGDTTGTIVGEVPNPTPF
                     RAFGAGSYHDFYVGYDVARKRDVFYGAGLGGYSAWDVTHPEAPVQLFTITGMGLDIAH
                     TFTPSPDGNYAVTETEYQYTPLKVWDLREGQDGTRKNLDLPISAWTADWHDLSHNHEV
                     RWPYVFVSAYEDGLFVFDLKDPKNPKTDGWYYTCTTCVHGMGFGGNPDNGWQGTNSVE
                     QGAFGVDVRNADGLIVLSDMRTGLWLFRMKGFTGWNGASVGQPNISSVQDFDHGPTGA
                     AASK"
BASE COUNT          754 a         1650 c         1763 g          795 t
ORIGIN      
        1 gccgtacccc tgcccgatga gcccggaggg gcggaggagt tcgtccatcg gcgaggtggc
       61 gcgggccgcg aggggcaggc ggtagtatgg aagcccgtag gcgaaggtcg tgaccgccac
      121 gccgagccag acggtgacga ggagccattc gaagacccgc tccccgatcg agcgcgaatc
      181 gtcgaggtcg aggctggggc ggcggccggc acgcgcccgc gcgccggcga tggtcggcaa
      241 ggatgacggg tccatggcga catcgcgacc gtccattcgc gcctccctcc ttatcggcat
      301 cggcggcacg ccaggcccac cgggacagtc agtagagacc cggaactcaa gtcacgttcc
      361 gtgcctatcg aatgcttcac gatcctggcg ccttcttcct tatctatagc atagcggagg
      421 gcgtccggcg gaagatgaag ggaccgggct cctcgctctg tgacatctga ccattcgatg
      481 ctggcgccgc tctgtcgtgc gggcggtgcg ttctgccaca gcgaagtcgc gcatacttga
      541 gcgcggcttc acattccagc gaggccattc gcgcgatcgt gcatcgccgt tcgacgcccg
      601 agccgggcag cgatgcacac aggaccgaag ggacgggtcc ggcgacatga aggcactggc
      661 cacggtcatc cggcgggcac cccggagcgc ggcggcgatc gcgttcgggg cgtgcaccgc
      721 cacgctcgcg cacttcgcat ggcacgccga cgtgcgcctg gacggcacga tcccggtcct
      781 tacgctcggc gcggggctcg cccacgcaat ggccggggtg ctcatcggtc gccggctcct
      841 cgacactgcg cgcaccccgt ccgcggccaa cgccgcgctc ttgggcgctg gcgcgtcgct
      901 catcgcccag gcgctgttcg tgccggtgct ggcggcctat gtcgccgaga gtggtgcggc
      961 gccgaccatc atcggctacg tgggtctcac gatctacgtc gcgatcttcg cgttcctggc
     1021 ggcggggtgg gcgatgctcc tgctctgcat ggcggtcgga tgggggctgt acaaggtcgc
     1081 agcacccccg gcggccggtt gaccgcgtgg ggcaggaccg gatccgggat ccggtggcgc
     1141 cgagacgcac cgaccgccca cggccaccgg tgagcggccg tgcagtgtcg gtcggacagg
     1201 gatcggcggg gagccggact tagcggcggg gatccacgtg cagcgtcatc atcatccccg
     1261 atcccagatg ctcggcgatg tggcagtgca gcatccaggt gcctgggttc gaggcgtcga
     1321 tgagcacgtc gacggtcgag ccgaccggga tcagcaccgt gtccttccac accaggtcgg
     1381 tggtccgcac gccgtcccgc gacaccacca gcatccgctg gccgtgcagg tggatggggt
     1441 gctgcatcgg gtggaaggaa tgcgggtcgt tgaagatgcg cagcttgacc accgacccct
     1501 ggcgcacgtg ccagtcgatg gccccgttct ccgcgccggt cgagtcgtcc cgcaggatcc
     1561 accgcacctc gttcgccgtc gagagccagt tcatctccgg catcccgtcg gtccattcga
     1621 ccggatggaa gtacgccgtg tccacgctca tgaactgcac cgtcgggagc ggaagcgatg
     1681 acgtggccac cgtcagggtg atccgcttgt caggcggccg gtcaaagtac tgccggtact
     1741 tcccgatctc ggccgtcacc gccgggttgg cacggagcgc ggcgaacgcc gcccggtagt
     1801 cgggcgcggc gggggtcgaa tccacggtgg cccagccgag cgtgtccacc tgggtctcga
     1861 actcaccgcg atagtggtcg atggcctgca ccgcgttcac gatcgggtac cgccccggcc
     1921 ggtcgaactc ggccgccacc acgtaccgct ccgccggcgc caggatcacg ctcgccacca
     1981 tggcctcccg ctcgaaacgg cccacgtcgg aggccagcac cttgagcggc acacccggga
     2041 tcgagaggtt ccaggtccgc gcgttggccg cgtcggtcag gaagaagcgc accacatcgc
     2101 cccgatggac ggtcagctga aagtcgggct cgccgttgac gagcggcacg ttccccaccc
     2161 gtcccatcag gacgaagtcg gacgcttcct tcccgaaggg gatgagggtg tcggcgtgga
     2221 tcaggagatc gctcagcatc agggcgacct cccggttggc gggggagtag tacgcggtgt
     2281 cggggctctc caccagcatg ttaccgtaga gccccatcga ctgctcgatg tcctcccgca
     2341 cgtgcgggtg gtaccagtag atccctgcat ccgggaagtg caccgtgtag gtgaagtccc
     2401 cgcctggcgc caccgggtcc tgcgtgacgc ctggcactcc gtccgaccgg ttgtcgagcc
     2461 gtacgccgtg ccagtgcacc gtgctcggca ggtcgatccg gttgtggaag cggacggtga
     2521 tggtggcgtt ctgcgccacc cggatcatcg gccccgggac ctgaccgttg aagccgtaca
     2581 tgacgaaggt gcggccccgg atggtgcggc ggaggagccc ggcggtgagg tcgagggtgt
     2641 cgccgtcgtt gagtggcacg atcgtcgagg ggagcgcgcg ggggaccgtc gccggatcca
     2701 cgccgccacc cggcaggaac gctccgacgg gcggcatgag cccgacaagc cccggaagca
     2761 tgatcatccc gcggggcatc gggatcggca tccggtccgc cgaatcggcg cccatctgca
     2821 ttcccgccat tccggggtct tgtgcggtca gcgcgaccgc gccgagggcg aacgcaaggg
     2881 cggtcaggag cgcgcgccgg atcatcccgg tgcgccgcgg cagaggggat gcgaaaggac
     2941 ttgctgcagc cagccgctcg gcgaggtgcc gtgcagcacc gtcggtcccg cgtgcgccgc
     3001 gggggtcgga tcggcctcgg cggtgatgac gaagaggaac ttgttccgct ccgccacgcc
     3061 gacggtgctg acgccattgg cgatgacgcc gagacggcgc cagtcggaga ggtcggggct
     3121 cacctcccag gccacgtagg cgcgatatgg gccgagcgtg gccggcgcgg gaagccccga
     3181 cgccatgacc cggatgtcgt agcggaggcg gccgtcggca gtggccgcga cgccgaacgg
     3241 ggagtcggca aagaccaggc gcgccgagcc ccgcgcggaa gggaccgcgg agcccggcgc
     3301 cagcgccatg tcgtacgggc cgggcttggg cccggcgctg gcaacggcca gcgccaggca
     3361 gcccagtact gcggatgccc tcacttcgac gccgcagctc ccgtgggtcc gtggtcgaag
     3421 tcctgcacgc tcgagatgtt cggctggccg acgctggcgc cgttccagcc ggtgaagccc
     3481 ttcatccgga agagccagag gccggtccgc atgtcgctga ggacgatgag gccgtccgca
     3541 ttccggacgt ccacgccgaa tgccccctgc tccacgctgt tggtcccctg ccagccgttg
     3601 tccgggttcc cgccgaagcc catcccgtgc acgcaggtgg tgcaggtgta gtaccagccg
     3661 tcggtcttcg ggttcttcgg atccttgagg tcgaagacga acaggccgtc ctcgtacgcc
     3721 gagacgaaca cgtacggcca ccggacctcg tggttgtgcg agaggtcgtg ccagtccgcg
     3781 gtccaggccg agatcgggag gtcgaggttc ttgcgggtgc cgtcctggcc ctcgcggagg
     3841 tcccagacct tgagcggggt gtactggtac tcggtctcgg tgacggcgta gttcccgtcg
     3901 ggggaggggg tgaacgtgtg cgcgatgtcg aggcccatcc ccgtgatcgt gaagagctgg
     3961 accggggcct ccgggtgcgt cacgtcccac gcggaatagc cgccgaggcc cgcaccatag
     4021 aagacgtcgc gcttgcgggc cacgtcgtag cccacataga agtcgtggta gctgccggcg
     4081 ccgaaggcgc ggaacggggt cgggttcggc acctcgccca cgatggtgcc ggtggtgtcg
     4141 ccggccacga ccttggccag gtcatagatg accgacttgg cttggttgac cgtcacgaag
     4201 tacagcaccc tgccgtcgga gtgcttgtag acgaaggtgt tgtggaaccc gcccggggcg
     4261 gccggatatc ggatccgcgc caccaccttg acggtggacg ggtcgggaag gccggtcacg
     4321 tcgaggatca cggcccccag gtcggcgtcg gggctcccct gcatgaactg gtacgactgg
     4381 gcgtagtagt agcgatcgcc gatcttgaag gccttgccgt ccatggcgcc gatgccgcgg
     4441 tggaggtcgg gattctcgat cgaccagtcg acgatcttct tcgggtggct cgggtccttg
     4501 atgtcgtaga tctgcacgtc gttgttgacg aagccgcaca cgtacacgta cggccggttc
     4561 atgtcctgct cgatctcgac gtccgcgagc ttccaggcgc cgggatgggt caccacgtgg
     4621 gcgacgacct tgatgttctt cgacccgccg atcttgtggt cggtggggac cttggcgccg
     4681 gggtcctgcg ccgaagcggt cgcggcgaca cccaacaggg caagggcgaa caatgctccg
     4741 cgcatcgtgt ctcctcggat gacgagtcgt gatgttcgtg aaggggtggc ccgggaatat
     4801 ggacagggcc gcgccccctg accagactcg gcgcgccatt gttccacctc tggacgccgg
     4861 ccgcacaggc agatagactg cgcgctctcc ctcacgcacg gtccccgtga ctgcaaccgt
     4921 cgctgcgctc gacttccccc ttcgcggaac gtggacggcg ct
//
LOCUS       DASUAP010000203         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1137035, whole genome shotgun sequence.
ACCESSION   DASUAP010000203 DASUAP010000000
VERSION     DASUAP010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137035"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1286)
                     /locus_tag="VFU45_05190"
     CDS             complement(132..1286)
                     /locus_tag="VFU45_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358488.1"
                     /translation="MATAFAPWAAAAQGSPFSGYYQNVGTVTGRIGLGGTALADLQRL
                     RLMLAPAWGPADAEVALEHTFTWENAGSATPAFGLLSSTRAGDDWLGIDWTIHNGPTV
                     VWRQRVDRLNVGWSTAAWSVRVGRQAISWATTLFLTPADPFVPFDPADPFREYRRGVD
                     AVRAQWYPSALSVVDVVIRPASTPYGDTFTALARSKATVLGWEVSAWGGEVQGAAGAG
                     AGLTRSVGASALRGELALRKDSSGGLVVRGAVGLDRRVTVAGRDLYGVIEYQHDGYGA
                     ANAGELPAVALSRPAQRAELQVLGRDEAAAQLTYQLHPLLGIELLALWNLRDGSGLVA
                     PAASFSVSDEVTARGGVFLAVGRSGPSLVAPASEYGPLPAGGYVAVSAFF"
     gene            complement(1310..2029)
                     /locus_tag="VFU45_05195"
     CDS             complement(1310..2029)
                     /locus_tag="VFU45_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358489.1"
                     /translation="MMPNDDVAVHTDGVWKVYPQEPRPVEAVRGVSLDVAAGDFVALA
                     GPSGSGKTTLLNMLGGLTRPTRGTVRLRGIELAGLSAAELARVRLEHVGFVFQAYNLL
                     PVLSALENAEFTLLLRGVPTAERERRVLDLFGRIGMTGMEHRRPAKLSGGQQQRVAVA
                     RAVVGGPALVLADEPTANLDSATGEALLDMMEELNRAEGTTFVFSTHDPRVMRRARRL
                     IHLVDGAIARDERTAPVERGA"
     gene            complement(2054..3313)
                     /locus_tag="VFU45_05200"
     CDS             complement(2054..3313)
                     /locus_tag="VFU45_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5358490.1"
                     /translation="MKRAAPLLRIAARNVMRQRRRSALIAAAMTLGVAALAIFLALGD
                     GMHAQWIDAGVRLADGHVTLEAPGYRPGMDIADRLDSAALGVTLAALARPDLTKDIRA
                     AAPRLAVDALASSATSALPVRALGVDPEAEGRFSALAEHLVEGRPLRQGDRLEALVGA
                     GLAERLHLHPGGRFVLAAPDRTGAVQEQLVHVAGVFRTGIREADQGVVDLPIETVRRW
                     LGMPGAATGVAVLLERGRSAVAVAGELRRLLPSGIAVRTWHETAPELDAAIRVDNLGN
                     YLFNGILLAIVSLAVLEALLVAVLHRRREFGVLQALGLTARETSLVVLGEGMLLATVS
                     GLAGLALGFGVTSILSRTGIDLSHVMGDQLSISGALVSPVVHPAAQLSRAWQGAVVVV
                     VLGLVASLYPMWQAARIDVADAVKLEA"
     gene            complement(3310..>4178)
                     /locus_tag="VFU45_05205"
     CDS             complement(3310..>4178)
                     /locus_tag="VFU45_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5358491.1"
                     /translation="GVSRVLQAIVAGSAPAPGSRTLALGDETARKLDVRPGDTVVVVA
                     PAADGSLGNDLYGVSGVFHTGLAPLDAGVAVLPLAAMQSLLALAPGRVHEVAVTVRDP
                     WSAPAVARALAADPALGRLGVTVEPWTTFRPELAQYARLAQAWNGILIAIVFLMAIFG
                     VTNTLLMSTFERRREFAVEGALGAGRAAIARTVVYEGLMLGAVSLAVGGALAAGVVVW
                     WHAAPLDLSRFMGNFTVSGALVRPVLRAVPSWQAPLEAAVALLVTSLLAALYPAFRAT
                     RIPPADALAGRE"
BASE COUNT          687 a         1605 c         1380 g          506 t
ORIGIN      
        1 acccatcatc cggcccctcg tgcggagact cctgaaggac attcccgcgg gaacgatccg
       61 cgaatgggcg ctgacgcacg acgaaggggg ataccggatc aggctctact agtaggggct
      121 cgccgcgtcc gctagaaaaa cgcactcacc gccacatacc cccccgctgg caacggcccg
      181 tactccgacg ccggagcaac caggctgggc cccgaccgcc ccaccgcgag aaatacgccc
      241 ccccgcgccg tgacctcgtc actcaccgag aacgacgccg cgggtgccac cagcccgctg
      301 ccatcccgca gattccacaa cgccaacagc tcgatcccca ggagcggatg cagctggtag
      361 gtcagttgtg ccgccgcctc gtcccggccc agcacctgca actcggcacg ctgcgcagga
      421 cgcgacaagg ccaccgccgg cagctctccc gcattcgccg ccccataccc gtcatgctgg
      481 tactcgatca cgccgtacaa atcgcgcccc gcgactgtca cccggcgatc gagaccgact
      541 gcgccacgca ccaccaaccc gccgctcgag tccttgcgca acgccagctc cccacggagc
      601 gccgaggcac ctaccgaacg cgtgagcccg gcgccggccc ccgccgcccc ctgcacctca
      661 cccccccacg ccgacacctc ccagccgagc accgtcgcct tgctgcgggc cagcgccgtg
      721 aacgtgtccc catacggcgt gctcgcaggc ctgatcacca catccacgac ggacagggca
      781 ctgggatacc actgcgcccg caccgcatcc acgccgcggc ggtactcgcg gaaagggtcc
      841 gccgggtcga acggcacgaa cgggtccgcg ggcgtgagaa acaacgtcgt ggcccaggag
      901 atcgcctgcc gcccgacgcg cacggaccac gcggcggtcg accacccgac gttgagccgg
      961 tccacgcgtt gccgccaaac caccgtgggg ccgttgtgaa tcgtccagtc aatgccaagc
     1021 cagtcgtctc ccgcccgcgt gctcgagagc aagccgaacg ccggtgtcgc actcccagcg
     1081 ttctcccacg tgaacgtgtg ctcgagcgcc acctcggcat ccgccggccc ccacgccggt
     1141 gcgagcatga ggcgcagtcg ctggaggtcg gcgagcgccg tgccccccaa cccgattcgg
     1201 ccggtgaccg tcccgacgtt ctggtagtaa ccggagaagg gagatccctg cgctgccgcc
     1261 gcccacggag cgaaggcggt cgccaagccc agtgcggcca gcgtcccttt cacgcgccgc
     1321 gctcgaccgg cgccgttcgc tcgtcccgcg cgatcgcgcc gtccaccaga tggatcagcc
     1381 gccgcgctcg ccgcatcacc cggggatcgt gcgtcgagaa cacgaacgtg gtcccctccg
     1441 cgcggttcaa ctcctccatc atgtcgagca acgcttctcc cgtcgcactg tcgaggttgg
     1501 ccgtcggttc gtccgccagc accagtgccg gcccgcccac caccgcgcgc gccaccgcca
     1561 cgcgctgctg ctgtcccccc gacagcttcg cggggcgccg gtgctccatc ccggtcatcc
     1621 cgatgcgccc gaacaggtcc agcacgcgcc gctcccgctc cgcggtgggg acaccacgca
     1681 acagcaacgt gaactcggcg ttctcgagcg cggagagtac ggggagcagg ttgtacgcct
     1741 gaaacacgaa acccacgtgc tcgagccgca cccgggccag ctcggccgcg gaaagcccgg
     1801 cgagctcgat cccacgcaac cgcaccgtgc cccgcgtggg gcgggtgagc ccgcccagca
     1861 tgttgagcag agtcgttttc ccggatcctg aaggcccggc gagggcgacg aaatccccgg
     1921 cggcgacgtc cagtgacacg ccgcgtaccg cttcgaccgg cctcggctcc tgcggataga
     1981 ccttccatac gccgtccgtg tggaccgcga catcgtcgtt cggcatcatc gtcacctttc
     2041 gcttctcatg aaatcacgcc tcgagtttca cggcatccgc cacatcgatg cgcgccgcct
     2101 gccacatcgg atagagcgac gcgaccaggc ccagcactac cactaccacg gcaccctgcc
     2161 aggcacgcga cagctgcgcc gccgggtgta ccaccggact caccagggcc cccgagatcg
     2221 acagctggtc acccatcacg tgcgacaggt cgatgccggt gcgtgagagt atcgaggtga
     2281 ccccgaagcc gagcgccagt ccggcgagcc cgctcaccgt ggcgagcagc atgccctctc
     2341 ccaacacgac cagcgacgtc tcccgggccg tgaggccgag cgcctgcaac acgccgaact
     2401 cccgacggcg gtggagcacc gctacgagca gcgcttcgag cacggccagc gacacgatcg
     2461 cgagcagaat gccgttgaag aggtagttgc ccaggttgtc cacgcggatc gccgcgtcca
     2521 gctcgggggc cgtctcgtgc cacgtgcgca ccgcgattcc tgacgggagc aggcgcctga
     2581 gctcgcccgc gacggccacg gcgctgcggc cgcgctcgag cagaaccgcc acgccggtgg
     2641 ccgcgcccgg catccccagc cagcggcgca ccgtttcgat gggcaggtcc acgacaccct
     2701 ggtccgcctc gcggatgccg gtccggaaca cgcccgccac gtggaccagc tgctcctgca
     2761 cggcaccggt ccggtccggt gcggcaagca cgaagcgccc accggggtgg aggtgaagcc
     2821 gctccgcgag cccggcgccc acgagcgcct cgagccggtc gccctgacgg agtggccgtc
     2881 cttccaccag gtgttcggcc aacgcggaga agcgcccctc ggcctcgggg tccacgccga
     2941 gggcccgtac gggaagggca gaggtggcgg agctggcgag cgcatccacg gccaggcgcg
     3001 gtgccgctgc gcggatgtcc ttggtcaggt cggggcgcgc cagcgcggcg agcgtcactc
     3061 cgagggcggc cgagtcgagc cggtcggcga tgtccatccc cgggcggtat cccggcgctt
     3121 cgagcgtgac gtggccatcc gccaaccgga cgcccgcgtc gatccactgg gcgtgcatgc
     3181 cgtcacccag cgccaggaaa atggcgagcg cggccacgcc gagcgtcatg gccgccgcga
     3241 tcaaggcgct gcgccggcgc tgccgcatca cgttacgcgc cgcgatccgg aggagcggtg
     3301 ccgcccgctt cattcgcgcc ccgcgagcgc gtcggcgggc gggatgcgcg tggcccggaa
     3361 cgcggggtag agggcggcga ggagggacgt gacgagcagg gccacggcgg cctcgagggg
     3421 cgcctgccac gagggcacgg cgcgcagcac aggacgcacc agggcacccg agacggtgaa
     3481 gttccccatg aagcgcgaca ggtcgagcgg ggccgcgtgc caccagacca ccactcctgc
     3541 ggcgagcgct ccacccacgg cgagcgacac cgcgccgagc atcagtcctt cgtagaccac
     3601 agtacgggcg atcgcggcgc gccccgcgcc aagggcgccc tcgacggcga actcgcggcg
     3661 ccgctcgaag gtgctcatca gcagtgtatt ggtgacgccg aagatcgcca tcaggaacac
     3721 gattgcgatc aggatgccgt tccaggcttg ggcgaggcgg gcgtactgcg ccagctccgg
     3781 ccggaaggtg gtccagggct cgaccgtcac accgagccgg ccgagggccg ggtctgcggc
     3841 aagggcgcgc gccacggcgg gtgccgacca ggggtcgcgg accgtgacgg cgacctcgtg
     3901 cacgcggccg ggcgcgagcg ccaacagcga ctgcatggcg gcgagcggga gcacggcgac
     3961 gcccgcgtcg agcggcgcca ggccggtatg aaacacgccg cttacgccgt agagatcgtt
     4021 accgagcgat ccgtcggcag ccggggccac gaccaccacg gtgtcgccgg gtcggacgtc
     4081 cagcttgcgc gcggtttcgt cgcccagggc gagggtgcgg gaccccggcg cgggcgcgga
     4141 gcccgcgacg atcgcctgga gcacgcgcga gacgcctc
//
LOCUS       DASUAP010000204         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_332822, whole genome shotgun sequence.
ACCESSION   DASUAP010000204 DASUAP010000000
VERSION     DASUAP010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332822"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFU45_05210"
     CDS             <1..395
                     /locus_tag="VFU45_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU5358492.1"
                     /translation="TDLMVSQIEQLAAGRAIEKPTYDYSQHTRAALTERVEPRDVVLV
                     DGIMLFVDQRLRSLFDIKIYVDVAERGRSVDSIIKQYLSTVRPMHLEFVEPSRHWADI
                     IVPEGGHNRIGIEMIQSRVVLEVARRRA"
     gene            complement(546..1238)
                     /locus_tag="VFU45_05215"
     CDS             complement(546..1238)
                     /locus_tag="VFU45_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358493.1"
                     /translation="MGTFEFLPSTRRGFVGRTAAALALSLTTSLPRLAEAEAPLRASD
                     PGTPSDEWMKRLTGKHKQIFDMKTHANGMGLLHVRNYLNTWRDAYHLTDKDVTPVLTF
                     YGFGLPMGFDDAAWAKYPFGDAMKVTDPTTNAPAKRNVFYKPVAGDTIAAGMLDSSID
                     QLVARGTVCIMCNNALAFWTMQLEKGGAGKAADIRADLVQHLLPGVSLVPGMVIAINQ
                     AQERGCAYMALG"
     gene            complement(1270..2169)
                     /locus_tag="VFU45_05220"
     CDS             complement(1270..2169)
                     /locus_tag="VFU45_05220"
                     /inference="COORDINATES: protein motif:HMM:NF044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5358494.1"
                     /translation="MISSPVGSVIARREQDRVARVWGRLRPLARQLACVAMAVALASC
                     RKATPAPAARLTLALPDTIIPAGPFGASILRGRALLTATRDSLPTHVGNRLRCVSCHL
                     DEGRRAQGTWVGVYARYPQYRSRAAAIQTIEGRVNDCFERSLNGTALPSDGRDMADIV
                     SYFWWLSRGTAVAAPSARAGSRFAGLTADTAAGRAVFGTICSVCHGAAGQGTAVAPPV
                     WGDSSFNIAAGMARPSTAAAFIRANMPFNAPGTLTDQQAFDVAAYVTGQRRPDFPAKI
                     DDWPLGDPPADVPYRTRAGRRAQ"
     gene            2339..>2722
                     /locus_tag="VFU45_05225"
     CDS             2339..>2722
                     /locus_tag="VFU45_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5358495.1"
                     /translation="MPSDHQRTRQELIHELVALRKQVADLRQAAHERQRVEEALRASE
                     ARYRAWLEELPVATARLDADGRLAGANEALAQLLGYAHRGELVELAPVLGLWAARDQE
                     LRLAPFLRERRAFHGELSLRARDGDI"
BASE COUNT          434 a          942 c          951 g          395 t
ORIGIN      
        1 acaccgacct gatggtgtcc cagatcgaac agctggcggc gggccgggcc atcgagaagc
       61 cgacgtacga ctattcccag cacacccgcg cggcactgac cgagcgggtg gagccgcggg
      121 acgtggtgct ggtcgacggg atcatgctct tcgtcgacca gcggctccgc tccctcttcg
      181 acatcaagat ctacgtcgac gtggccgagc ggggccgctc ggtcgactcg atcatcaagc
      241 agtacctctc caccgtccgg ccgatgcacc tggagttcgt ggagccctcc cggcactggg
      301 ccgacatcat cgtgccggag gggggccaca accggatcgg gatcgagatg atccagtcac
      361 gggtggtgct cgaagtggcg cggcggagag cttagagccg agagcttaga gcctggagcc
      421 aagagagaag agatcacagt cgcagatcgg gaacgggaaa tcccgatcca agactctacg
      481 ctccaagctc tgagctctaa gctctaagct ctaagctcca agctctaagc tccaagctcc
      541 actcctcacc ccagcgccat gtacgcgcac ccccgctcct gcgcctggtt gatggcgatg
      601 accatccccg gcaccagcga cacccccggc agcaggtgct gcaccaggtc ggcccggatg
      661 tcggccgcct tccccgcccc gcccttctcc agctgcatcg tccagaacgc cagcgcgttg
      721 ttgcacatga tgcacaccgt gccacgggcc acgagctggt cgatggacga gtccagcatc
      781 ccggccgcga tggtgtcgcc cgccaccggc ttgtagaaca cgttgcgctt ggccggtgca
      841 ttggtcgtcg ggtcggtcac cttcatcgcg tcgccgaagg ggtacttggc ccatgccgcg
      901 tcatcgaacc ccatcggcag gccgaacccg tagaacgtga gcaccggcgt cacgtccttg
      961 tccgtaaggt gatacgcatc gcgccaggtg ttgaggtagt tccgcacgtg gaggaggccc
     1021 atcccgttcg cgtgcgtctt catgtcgaag atctgcttgt gcttccccgt gagccgcttc
     1081 atccactcat cgctcggcgt gccggggtcg gacgcgcgga ggggggcctc cgcctccgcc
     1141 aggcggggca gcgaggtcgt gagcgacagc gcgagcgccg ccgccgtgcg gccgacgaag
     1201 ccgcgacggg tggaaggcag gaattcgaat gtgcccatcg gtacctccgg attgtgggtt
     1261 ggtggatcgc tattgggcgc gccggccggc gcgtgtccgg taggggacat cggccggggg
     1321 atcgcccagg ggccaatcat cgatcttggc cgggaaatcg gggcgtcgct gacctgtcac
     1381 atacgccgcc acgtcgaatg cctgctggtc ggtgagggtg ccgggggcgt tgaacggcat
     1441 gttggcgcgg atgaacgccg ccgccgtgct gggccgcgcc atcccggcgg cgatgttgaa
     1501 ggagctgtcg ccccagaccg gcggcgccac cgcggtgccc tgcccggccg cgccgtggca
     1561 gacggagcag atcgtgccga agaccgcgcg ccccgcagcc gtgtccgcgg tgagccccgc
     1621 aaatcggctc ccggccctgg ccgacggcgc ggccacggcg gtcccccgcg aaagccacca
     1681 gaagtacgac acgatgtcgg ccatgtcgcg cccgtcggag ggcagcgcgg tgccgttgag
     1741 tgaccgctcg aaacagtcgt tgacccggcc ttcgatcgtc tggatcgcgg cggcgcggct
     1801 gcggtactgg ggatagcgcg cgtacacgcc gacccaggtc ccctgcgccc gccgcccctc
     1861 gtcgagatga cagcttacgc agcggagccg gttcccgacg tgggtgggga ggctgtcccg
     1921 ggtggcagtg aggagcgccc gaccgcggag gatcgacgca ccgaacggac cggccgggat
     1981 gatggtatcg ggcagggcca gggtgagtcg cgccgcagga gcgggcgtgg ccttccggca
     2041 cgacgcgagc gccacggcca tcgccacgca tgcgagctgc cgcgcgaggg gccggaggcg
     2101 gccccacaca cgcgcgacac ggtcctgctc gcgccgggcg atcacggaac ccaccggaga
     2161 ggagatcacg tcaccaagtt gcaggccgtg acgatttccg tccagagtgg cgcctgggcc
     2221 acagccgggg cgcccactgg gtgtcgggat ggagcgagtt cggccatcgg ggccgaggcg
     2281 tgcggacact gtgcaggtcg ggcgccctgc ggcatccaac tgggcaacgg acggcccgat
     2341 gcccagcgac caccagcgca cccgccagga actgatccac gaactcgtcg ccctgcggaa
     2401 gcaggtcgcc gacctccgcc aggccgccca cgagcgccag cgagtggagg aggcgctgcg
     2461 ggcgtcggag gcgcgttatc gcgcctggct cgaggaactc ccggtggcca cggcccggct
     2521 ggatgccgac ggccgcctcg cgggggccaa tgaggcactg gcccagctcc tgggctacgc
     2581 ccaccgcggc gaactcgtcg aactggcgcc ggtgctcggc ctctgggccg cccgggacca
     2641 ggaactccgc ctcgcaccct tcctgcggga acgccgcgcg ttccacggtg agctctcgct
     2701 ccgggcccgg gatggcgaca tc
//
LOCUS       DASUAP010000205         6120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1286996, whole genome shotgun sequence.
ACCESSION   DASUAP010000205 DASUAP010000000
VERSION     DASUAP010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286996"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VFU45_05230"
     CDS             complement(<1..704)
                     /locus_tag="VFU45_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358496.1"
                     /translation="MTSSTLMISVSGMRGIVGTDLTPELVARHAAALGAWARHAGRPV
                     VVLGRDARTSGPMFARAAAAGLMSAGADVIDLGIVPTPTVQLAVEHHHAGAGLILTAS
                     HNPIEWNALKFVGPDGIFLDGEAGAAVRALAERGPDRAGWDGVGQVREDGAAIDRHLE
                     LVLKLPELDLKALRKRRFKVALDCVRGAGGTTIPRLLEALGCEVVAINLETDGRFPRA
                     PEPIPENLGDLRRLVR"
     gene            complement(739..1803)
                     /locus_tag="VFU45_05235"
     CDS             complement(739..1803)
                     /locus_tag="VFU45_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5358497.1"
                     /translation="MSTLQEPEPSAAAVPAARCLVVDDDDLVRHALVRVIEGQGLACV
                     QAGSGTEALELLERQGEVPVVISDIHMPEMDGLELLRRLRQRWPDTAVIMLTAVADVE
                     TAVSCMQLGAMDYIAKPVIVDEVRTRVTRALEKRELTLQNRFYQKHLETRVRQQAQRI
                     RELFLEGVQTLAHALEAKDSYTRGHSKRVSWYSVKTGVNLGFTGDELEELRLGAELHD
                     IGKIGTREDVLNKPGPLTAEEFRHIAEHTVLGERILAPLARETPRVLRIVRSHHERLN
                     GSGFPDGLAGDAIPLEARIVAVADAFDAMTTNRTYRQLRSTEVALEELRRCIGDQFDA
                     RVVDAFLRAFPDPAQLPVSF"
     gene            1908..3080
                     /locus_tag="VFU45_05240"
     CDS             1908..3080
                     /locus_tag="VFU45_05240"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU5358498.1"
                     /translation="MTRILPDDHAHGLSTRAVHAGQVPDPLAGAVMPPIYQTSTYIQE
                     GLGRHKGYEYARTANPTREALERNVAALEGATHGFAFGSGLAALDTLLKIFSQGDHVV
                     CGENVYGGSHRLMTRIFSRFGLAFSFVDTSDIGQVERAVTPKTRLVYCETPTNPMMTL
                     TDLRAVGDLTQARGLIFAVDNTFATPVFQRPIELGADVVLHSTTKYLNGHSDMVGGLL
                     ITSRDDLAEQVGFLQNAAGGVPGPMDCWLALRGIKTLALRMKRHDETGRQLAPWLAAH
                     KAVKKAYYPGLPDHPQHELARRQMQGFGGMISLELGDVAFARRVVERTRVFRLAESLG
                     GVESLIGHPASMTHASVPVPMRQAMGLTDSLIRLSCGIEDVEDLMADLEQAFAGAK"
     gene            3227..4246
                     /locus_tag="VFU45_05245"
     CDS             3227..4246
                     /locus_tag="VFU45_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU5358499.1"
                     /translation="MSSTLPAPRPRRTLPQIAPISWEHPADRAALQALRAVPGADEAI
                     KKVIGLLGGQRGIQLLFQANAIRVGPDQFPRLWQMHVEMCATFDWPKVPELYVTQTPI
                     FNAGAYGVDDPFIVIHSAALELLDDDELRGLLAHELGHVMSGHALYRTIAAVLALVSL
                     GALPFLAELVVLPVKLAFLEWSRKSELSADRAGLLGVQDPIAMQRKFMKMAGGGRGAW
                     FTMADMNVDAFMAQANEYAHNHEGFDVVYKVLNTLSVTHPMNTVRAAEIQAWVTAGEY
                     DRVLRGEYVRRGTPEAERPWAEDWKGAAEHYGKEAREMAQQVGKAARHAAQRVGEAIR
                     GARKG"
     gene            4243..5511
                     /gene="purD"
                     /locus_tag="VFU45_05250"
     CDS             4243..5511
                     /gene="purD"
                     /locus_tag="VFU45_05250"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU5358500.1"
                     /translation="MRLLVVGGGGREHALCWALRRENPHADIYAAPGNPGIGALATNL
                     GIPADDIDRIVDAADAYGIDLVVIGPEVPLAMGLADRLRGEGRTVFGPGAAAAQIEAS
                     KAFAKEIMHRAGVATADSATFTDLAPALAYVDSHPEPMVVKASGLAAGKGAVVCATRA
                     EAAQAVREMLGEKRFGSAGQVVVVEDFLDGEELSVFAVTNGREVRILPAAQDHKRLLE
                     GDKGPNTGGMGAYAPVSIATHDLLQMVERDVLLPTLAQLEDEGAPFNGLLYAGLMIGT
                     DGAPSVIEFNCRFGDPETQAILPLIDGGLTDLLDAAARGTALPTVRVRRGAAVTTVLA
                     APGYPDAPRRGAPITIPEDLPAGVTVFHAGTDISDGTLRVSGGRVLTVTGVGGSFAEA
                     QAASRAGAEAIQFEGKVWRRDIGWREAARA"
     gene            5628..6032
                     /locus_tag="VFU45_05255"
     CDS             5628..6032
                     /locus_tag="VFU45_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5358501.1"
                     /translation="MHRPTLLLAAATLGLGACASSSSGPSGIAADVTIVANASGLTTT
                     AFSPDSFTISLATKDTISWRNADLGTPGDAYGGGGTPGVSHHIVSDSTGVFDSGVMAP
                     GAFYAHAFTTPGKFHYHCSIHPGMVGVVVVTS"
BASE COUNT          902 a         2242 c         2118 g          858 t
ORIGIN      
        1 tcccggacca gccggcggag gtcacccagg ttctccggga tcggctcggg ggcgcggggg
       61 aagcggccgt cggtctcgag gttgatggcc acgacctcgc agccgagcgc ctcgaggagc
      121 cgggggatgg tggtgccccc cgcgccgcgc acgcagtcga gcgccacctt gaaccgccgc
      181 ttccggagcg ccttgaggtc gagttccggg agcttgagca ccagctccag gtggcggtcg
      241 atggcggcgc cgtcctcccg cacctggccc acgccgtccc accccgcgcg gtcgggcccc
      301 cgctcggcca gcgcgcggac cgccgccccc gcctcgccgt cgaggaagat cccgtcgggc
      361 cccacgaact tgagggcgtt ccactcgatc gggttgtggc tcgcggtgag gatcaggccg
      421 gcgccggcgt ggtggtgctc caccgccagc tggaccgtgg gcgtggggac gatgccgagg
      481 tcgatcacgt cggcgccggc ggacatgagc cccgccgccg ccgcgcgcgc gaacatcggg
      541 ccgctggtcc gcgcgtcgcg gccgagcacc acgacggggc ggccggcgtg ccgcgcccag
      601 gcgccgaggg cggcggcgtg gcgtgccacg agttcggggg tcaggtcggt gcccacgatg
      661 ccacgcatcc cggacacgct gatcatcaag gtggaagagg tcatcggcga gggtccgggg
      721 cacgcggccc cggcagggtc agaacgagac cggcaactgg gccggatcgg ggaaggcgcg
      781 gaggaaggcg tcgacgacgc gggcgtcgaa ctggtcgccg atgcagcgcc ggagctcctc
      841 gagcgccacc tcggtggacc ggagctgccg gtaggtgcgg ttggtggtca tcgcgtcgaa
      901 ggcatcggcc acggccacga tccgggcctc gagcgggatg gcatcgcccg cgagcccgtc
      961 ggggaagccg gagccgttga ggcgctcgtg gtgggagcgc acgatccgga ggacgcgcgg
     1021 cgtctcccgc gcgagcggcg ccaggatccg ctcccccagc acggtgtgct cggcgatgtg
     1081 gcggaactcc tcggcggtga gcgggcccgg cttgttgagc acgtcctccc gggtgccgat
     1141 cttgccgatg tcgtgcaatt cggcgccgag ccggagctct tcgagctcgt cgccggtgaa
     1201 gccgaggttg acgccggtct tgaccgagta ccagctcacc cgcttcgagt ggccgcgggt
     1261 gtaggagtcc ttggcctcga gcgcgtgcgc cagcgtctgc accccttcga ggaagagctc
     1321 gcggatccgc tgggcctgct gccggacgcg ggtctccagg tgcttctggt agaagcggtt
     1381 ctgcagggtg agctcgcgct tctcgagcgc gcgggtgacg cgggtgcgca cctcgtccac
     1441 gatgaccggc ttggcgatgt aatccatggc gccgagctgc atgcaggaga ccgccgtctc
     1501 gacgtcggcg acggccgtga gcatgatgac ggcggtgtcg ggccagcgct gccggagccg
     1561 ccgcagcagc tccaggccgt ccatctccgg catgtggatg tcgctgatga cgaccgggac
     1621 ctccccctgc cgctccagca gctcgagcgc ctccgtgccg gaccccgcct gcacgcatgc
     1681 cagcccctgc ccctcgatga cccggaccag cgcatgacgc accaggtcgt catcatccac
     1741 gaccaggcag cgggcggcgg gaacggcagc ggcggaaggt tcgggttcct gaagcgtgct
     1801 cacaacgcca agttacgcgc cccgcggagc gggcacaacc agcggcctcg ccttgccggc
     1861 ccggagaccg cgccgttagg ttccccgcgc ctccacccgg ccatccgatg acccgcatcc
     1921 tcccagacga ccacgcccac ggcctctcga cccgcgccgt tcacgccggc caggtgcccg
     1981 accccctcgc cggggcggtg atgccgccga tctaccagac ctcgacctac atccaggagg
     2041 ggctcggtcg gcacaagggc tacgaatacg cccggaccgc caacccgacc cgcgaggcgc
     2101 tggaacggaa tgtggccgcc ctggaggggg ccacgcacgg cttcgccttc gggtcggggc
     2161 tcgccgcgct cgacacgctc ctcaagatct tttcacaggg cgaccacgtc gtctgcggcg
     2221 agaacgtgta cggcggctcc caccggctca tgacccggat cttcagccgc ttcggcctcg
     2281 ccttctcctt cgtcgacacc agtgacatcg ggcaggtcga gcgcgcggtg acgccgaaga
     2341 cccggctggt ctactgcgag accccgacca acccgatgat gacgctcacc gacctccggg
     2401 ccgtgggcga cctcacccag gcgcggggtc tcatcttcgc ggtcgacaac accttcgcga
     2461 cgccggtctt ccagcggccg atcgagctgg gcgctgacgt cgtgctccat tccactacca
     2521 agtacctcaa cggccacagc gacatggtgg gtgggctcct catcacctcc cgcgacgacc
     2581 tggccgagca ggtgggcttc ctccagaacg ccgccggcgg cgttcccggc ccgatggact
     2641 gctggctcgc gctccgcggc atcaagacgc tcgcgctgcg gatgaagcgg cacgacgaga
     2701 ccggccgcca gttggcaccg tggctcgcgg cccacaaggc ggtgaagaag gcgtactacc
     2761 ccgggctccc cgaccacccg cagcacgaac tcgcgcggcg ccagatgcag ggcttcggcg
     2821 ggatgatctc cctcgagctc ggcgacgtcg ccttcgcccg acgggtggtg gagcggaccc
     2881 gcgtcttccg cctggcggaa tcgctcggcg gcgtcgagag cctcatcggc caccccgcct
     2941 cgatgaccca cgcctccgtc ccggtaccga tgcgacaggc catggggctc accgacagcc
     3001 tcatccgcct gagctgcggc atcgaggacg tcgaggacct gatggcggac ctggaacagg
     3061 cgttcgcggg ggcgaagtga ggtgagcggt gaggggtgag cggtgagcgg gaggggcgcg
     3121 caactcgctc ccgttcaccg ttcaccgctg accgttcacc gttcctatct gaaacctctc
     3181 cccggccgac ccgtagggcc ggatgtcccc ttcagtcgag cgttgaatga gcagcaccct
     3241 gcccgcccca cggccccgcc gtacgctgcc gcagatcgcc cccatctcct gggaacaccc
     3301 cgccgaccgc gcggccctgc aggccctcag ggcggtcccc ggggcggatg aggccatcaa
     3361 gaaggtcatc ggcctcctcg gcggccagcg cggcatccag ctcctcttcc aggccaacgc
     3421 catccgcgtg gggcccgatc agttcccccg cctctggcag atgcacgtcg agatgtgcgc
     3481 caccttcgac tggccgaagg tgccggagct ctacgtcacc cagaccccga tcttcaacgc
     3541 cggcgcgtac ggcgtcgacg atccgttcat cgtgatccac tccgccgccc tcgagctcct
     3601 ggacgacgac gagctccgcg gcctcctggc ccacgagctg ggacacgtga tgagcggcca
     3661 cgcgctctac cgcaccatcg ccgcagtgct ggcgctggtg agcctcggcg ccctcccgtt
     3721 cctggcggaa ctcgtggtgc tgccggtcaa gctggccttc ctcgaatgga gccggaagtc
     3781 cgagctctcc gccgaccgcg ctgggctcct cggcgtgcag gacccgatcg cgatgcagcg
     3841 gaagttcatg aagatggcgg ggggcggacg cggcgcctgg ttcacgatgg ccgacatgaa
     3901 cgtcgacgca ttcatggcgc aggccaacga gtacgcccac aaccacgaag ggttcgacgt
     3961 ggtgtacaag gtgctcaaca ccctcagcgt gacccacccg atgaacaccg tgcgcgccgc
     4021 cgagatccag gcatgggtga ccgcagggga gtacgatcgc gtcctgcggg gtgaatacgt
     4081 ccgccgcggc acgcccgagg ccgagcggcc ctgggcggag gactggaagg gcgcggcgga
     4141 gcactacggg aaagaagcgc gggagatggc acagcaggtc gggaaggcgg cgcgtcatgc
     4201 cgcccagcgc gtgggcgagg cgatccgcgg cgcgcggaag ggatgaggct cctcgtcgtc
     4261 ggcggcggag gccgcgagca cgcgctctgc tgggccctcc gccgcgagaa cccccacgcc
     4321 gacatctacg ccgcccccgg caaccccggc atcggcgccc tcgccaccaa cctcggtatc
     4381 cccgccgacg acatcgaccg catcgtcgat gccgccgacg cctacggcat cgacctcgtg
     4441 gtcatcggac cggaggtgcc gctcgcgatg ggactcgccg accggctccg cggcgagggg
     4501 cggaccgtgt tcgggccggg cgcggcggcg gcccagatcg aggcgtccaa ggcgttcgcc
     4561 aaggagatca tgcaccgcgc cggcgtggcc accgcggaca gcgccacgtt caccgacctg
     4621 gccccggcgc tggcgtacgt ggattcccat cccgaaccga tggtggtcaa ggcctccggg
     4681 ctcgcggcgg gcaagggcgc cgtcgtctgc gccacgcgcg cagaggccgc ccaggcggtg
     4741 cgggaaatgc tgggcgagaa gcgtttcggc agcgcgggcc aggtggtggt ggtcgaggac
     4801 ttcctcgacg gcgaggagct ctcggtcttt gccgtcacca acgggcggga ggtgcggatc
     4861 ctgccggcgg cgcaggacca caagcgcctc ctcgagggcg acaagggccc caacaccggc
     4921 gggatgggcg cgtacgcgcc ggtctcgatc gcgacgcacg acctgctgca gatggtcgaa
     4981 cgcgacgtgc tgctcccgac cctggcgcag ctcgaggacg agggggcacc cttcaacggc
     5041 ctgctctacg ccggcctgat gatcggcacg gacggagcgc cttccgtcat cgagttcaac
     5101 tgccgcttcg gcgatccgga gacgcaggcg atcctgccgc tcatcgacgg cggcctcacc
     5161 gacctgctcg acgcggccgc acgcgggacc gcgctgccga cggtgcgcgt gcggcgggga
     5221 gcagccgtca ccaccgtgct ggcggcgccc gggtatcccg acgcgccacg ccgcggcgcg
     5281 ccgatcacga tccccgagga ccttcccgcc ggtgtcaccg tcttccacgc cggcaccgac
     5341 atcagcgacg gcacgctccg ggtcagtggc gggcgggtgc tcaccgtcac cggcgtgggc
     5401 gggagcttcg ccgaggccca ggccgccagc cgtgccggcg ccgaggcgat ccagttcgag
     5461 gggaaggtct ggcggcgcga catcggctgg cgcgaggcgg cgagggcctg accatccggc
     5521 acttccgtag tattgcggat atgacgggag ttacgggaac aaattgtggt gtaaccgcgt
     5581 cctccggacg tccttggtgg acattcacca cgcacccgga gcgccccgtg catcgtccca
     5641 ctctcctcct ggccgcggcc acgctcggcc tcggcgcctg cgcgtcgagc tcgtccggtc
     5701 cgtccggcat cgccgccgac gtgaccatcg tggccaacgc gtcaggcctc accaccaccg
     5761 cgttctcccc ggactccttc accatctcgc tcgccacgaa ggacaccatc agctggcgga
     5821 acgccgatct cggcaccccg ggcgatgcgt acggcggcgg cggcaccccc ggcgtctccc
     5881 accacatcgt gtccgactcc acgggtgtgt tcgattccgg cgtgatggcg cccggcgcat
     5941 tctacgccca cgcgttcacg acgccgggca agttccacta ccactgctcc atccatccgg
     6001 ggatggtcgg ggtcgtcgtc gtcacgagct gaccacgccc gcgggcgcgc ccccagccaa
     6061 cgagcggtga acggcggctc actcccccgt tcaccgcttt ccgtttaccg ttcaccgaca
//
LOCUS       DASUAP010000206        13400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_823387, whole genome shotgun sequence.
ACCESSION   DASUAP010000206 DASUAP010000000
VERSION     DASUAP010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 13400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13400
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823387"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..986
                     /locus_tag="VFU45_05260"
     CDS             144..986
                     /locus_tag="VFU45_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358502.1"
                     /translation="MRALLIGGLALLAACSSNAGAGTDVAPRDGHGRARPMAPVTIRP
                     APPARYAFYRQDSVTTRLPNGAESPQVVGRTLLLTVSITPPAAGTTGDARISIALDSI
                     AIDPGTPEMARAPFVGVAGTTWSGTVDGTGRISGLAPDRPSTGAEAVTPDLQRWLPVL
                     PAAGARPGDAWTDTVASVYPFSNLNLQVADTLIAAWTAKAGVAPRTLEIRDSGTIARK
                     GTSNIVTLMGGGTRTAQYTLGADGTLEAASGVDSVVMKISVLAVGQSVTVRQLGRYTV
                     TRRP"
     gene            991..2025
                     /locus_tag="VFU45_05265"
     CDS             991..2025
                     /locus_tag="VFU45_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEU5358503.1"
                     /translation="MTLSFRNLLRGVAGLAGAFVVWLFAVWPPPAWYRSHWPAETAFQ
                     EMRAQERGDAGAQGCRGAGGVAHQDSNRSFLRPRVSAALRPCARLYHPVPLDSIAPSM
                     VDAAMAGEDQRFLEHHGIDWVNLRRALGYDRDDFRWSSARDRGDLLRALPHAWARRDR
                     LRGASTITQQLAKNLYLSPSRNPLRKVKEAVTAWRLEAALDKDRIMALYLNTVELGHE
                     VWGVDAASRLYFKRPARQLTLAESAELAGELPFPLRSNPGYRPARMFHRQQLILAHLR
                     GEPVEIPPDSGLADVADTLVPAPTVESLPPMPEESLPQLLKTDSGGTAQVDSGAVGQR
                     DSVKDAPPRS"
     gene            complement(2138..3892)
                     /locus_tag="VFU45_05270"
     CDS             complement(2138..3892)
                     /locus_tag="VFU45_05270"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5358504.1"
                     /translation="MSRSTLLALAALLGAGCTPRVTTTPPAETSAPSPAATAGVQPAW
                     PDELPFRTLAPRPTTPDVTAADMMTRLYRFADDSMQGRESGELGNFKGTTYIAAEFAR
                     DGLTPMGEGGTFFQAIGLERGLFDSTLAFRSSAGPIRPWSDIVPLPPLGTAPFRPELN
                     VTDAPSVFAGTWGDPAGTPDAAGLKGKVAIFRLPGDGRSLGFWGASEPRAAGAAAVAV
                     VIPDSVMPFIASNFDHAREFLQSDRPAEGPLAAFISASAVARLFGHPVDSLSAGAAGA
                     AVTATVHYAVGPTAYPARNVIALLKGSDPRRNGEYVVISAHNDHVGMGRPADHDSIRA
                     YNRVMRPWGANDRPAPPTPEQWARIHALLDSARARRGGVVRMDSIYNGADDDGSGTVA
                     LLEIAEDLAHRPDRPARSILFVSHTGEEKGLLGSQWYTDHPTVPRDSIVVDLNMDMVG
                     KGRPTDMDGSPNSVQTIGSYRLSTDLGKLIDSLNTHRSEPMAINYTWDAHGNPLNRYC
                     RSDHFMYARYGIPITYFSLGYAIDYHQLTDEPQYVDYEHGAKVARFVRDIALAVADRP
                     ERLKVDGPIQDPKQPCRQ"
     gene            complement(3960..5072)
                     /gene="dnaN"
                     /locus_tag="VFU45_05275"
     CDS             complement(3960..5072)
                     /gene="dnaN"
                     /locus_tag="VFU45_05275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5358505.1"
                     /translation="MKFTITREQLHQGLTSVAASIPAKTTLPVLSNILVEASKDGLRL
                     SGTDLDIAVSTLIPASVDQEGAITLPAKKLQEIVRELPSGSIRVTSSGEQRVSIESGR
                     AKFKLLGLPREEYPAFPAVKFDGGWRCTSGDLQKLISHVAFAASTEESRPILNGVHWE
                     LRPDRMRMVATNGHRLAKMDIPLKTGVAEEAKLIVPPKALDQIRRLFAADEEIEIAKS
                     ENHLGFRSATTQVFTRLIEGPYPNYEQVIPRENDKVATADRASLTAALRRMSIVASDQ
                     THRIRMTLSNGACKLSVQTPDLGEAQEEVAVAYDGEALDIGFNATYLLEIFKYMPTDE
                     IRLTFKGPERAATCEPVGWDDPASYMTLVMPLRLVD"
     gene            complement(5324..6712)
                     /gene="dnaA"
                     /locus_tag="VFU45_05280"
     CDS             complement(5324..6712)
                     /gene="dnaA"
                     /locus_tag="VFU45_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU5358506.1"
                     /translation="MPQLSAKDAWSRILDEAQRQLPTEAMRSWLQPTEAVVLEDGQLT
                     VAAPDQFAVQWNQEKYASVLGRLAEGVVGRPTSVVFRVQEARLQRPQMDFFVQQARPA
                     GEVPGSQNQSQQPLNERYTFDTFVIGKSNELAAAAAHAVSESPGKTYNPLFIYGATGL
                     GKTHLMQAVAHAVVQRNPETRMLYLGAEQFMNEVIEGIGKGTMEQFRRRYRNGVDLLL
                     VDDVHFLQGKVQTQEEFFHTFNALYDNKKQVVLTSDRPPKELQGLEARLVTRFEWGMV
                     ADVGHPDLEHRIAILRKKQEQDHLETTIPDDVLRFIAEHVRSNVRELEGCIIKLLLFA
                     SLRHREVSIDLAREALADKIRDADDAAAAIRALPSIDKVQDVVARRWGVTTEGLRSKT
                     RIKTLTVPRQIAMYLARHLLEMPLVEIGQAFGGRDHSTVIHSVEKVERQMQRDRSFRD
                     RIEAARQELVTN"
     gene            6978..7139
                     /gene="rpmH"
                     /locus_tag="VFU45_05285"
     CDS             6978..7139
                     /gene="rpmH"
                     /locus_tag="VFU45_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU5358507.1"
                     /translation="MKPSYRPRNRRRINKHGFRARMATRWGRATLSRRRKKGRRQLTV
                     KLPSKHGGR"
     gene            7154..7480
                     /gene="rnpA"
                     /locus_tag="VFU45_05290"
     CDS             7154..7480
                     /gene="rnpA"
                     /locus_tag="VFU45_05290"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU5358508.1"
                     /translation="MRADRLARASELRRCLAHGRRRRGAHLEMIWLDNSSGHPRMGMI
                     VPRFQFSAVARNRLRRRIREVWRREVRPAQPGWDLVVRARREAYAAPFAALRGELLAW
                     REAVCG"
     gene            7549..7788
                     /gene="yidD"
                     /locus_tag="VFU45_05295"
     CDS             7549..7788
                     /gene="yidD"
                     /locus_tag="VFU45_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU5358509.1"
                     /translation="MRPSVSLRNWPRLAGWYLIRGYQRFISPMFPPSCRFTPSCSQYA
                     LEAVTRYGLLRGTWLGGRRLMRCHPFHPGGFDPVP"
     gene            7805..9517
                     /gene="yidC"
                     /locus_tag="VFU45_05300"
     CDS             7805..9517
                     /gene="yidC"
                     /locus_tag="VFU45_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU5358510.1"
                     /translation="MDRRIIAAVFLMMLVAVVPSLIFKAPPKAVTSQVADSAAVGQRD
                     SGTVGRGAALPQRPSAPAPLRPSAPAPLRTAAPAHDDSVVVTSGLYRYAFSTRGARLI
                     SATLPKYPSMATTAAPGTPVELVRPGGGLLALTLVSGRDTVPLSDWTFTPSARSVAVP
                     ADGTAPLTFTASQGGVTVTLGFTFRSADYRFDVAGKVEGLGPTGGFLLVGLGGGLPNT
                     EADTLANGRAHALVVKPVGKSTNLIPFSKIDPGTSREVPGPFRWVASKSEYFVVGLFA
                     TDTASPWTATRAVVPAGVGKYAGVAALEVSQPVAGPGTFGYQLYAGPMEYNRLSAIGQ
                     DFDDVNPYGIVGFRAVIRPFALFFRQVFVWMHTHLGISYGFAIILLGMLIRLVLWPLN
                     QKAMRSAMAMQAIQPHMKALQERHKSDPARLQQEMVKLYKEHNANPLGGCLPMLIPMP
                     ILFALFFVFESTIEMRGVPFWWFTDLSQPDPHHIIPFLTGLSMLLLSWIGQRSIPPNP
                     QAKMMMWMAPVMFTFFGFRFASGLNLYYVVSNLASVPQQWLLSEERRKRNPPAPPAPA
                     PGGR"
     gene            9655..>13400
                     /locus_tag="VFU45_05305"
     CDS             9655..>13400
                     /locus_tag="VFU45_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358511.1"
                     /translation="MPSSVLLASRARALGRQLLVAASALLLVTCTDNPTGPTVGSTLR
                     LAVSPRFKAGTFLSPALPVDQVRLVVYHQPACDCAADSIAGATVPFALTDTAVSLSVP
                     VRLTQPAESLTVQFYLMGGGVQLFFGSQAVVLTSGSTPVIPPVLLTYVGPGAGLDSLL
                     ITPRDSVLTFGDSLTFGVAAFQVGAPVSAFYVGWKSSLATAKILPDGKIHAPGARSKT
                     TVTVQAPNGKADSTTLTFVPKPVTLFKVSGDTQSAPRLDTLPLPLRVRVKAADSLGVG
                     GVRVVFHPLGFAGAAVPDTVYTDSLGFAQVRAVLSDSVGTQQWQATAAGLTAVTFSAN
                     SQVVAGPPYRVKLVTQPSDTTASGALIPVQPVLQVEDSVGVPVHVAGVVVTAQSLTYL
                     TGAPQPKRPLGGGLKPSSTRASAPYKSADTFGQISDSTDATGKVAFTGLRMVGYGAER
                     VVFNAPALLADTSVAMWVTAGAPKNLVKGSADSLTAYVDSLVPVAPAVYVYDSTYNPV
                     PNVAVVFQVTAGGGTIPKPVDTVFTDTTGLATLASWKLGPNPGTNTVQATVAGAGSLT
                     FTAFAQPPVPTLLLQLQGTSVVGVGRTATLQVKLSSPATATDTLYVTSDSTQTITVSG
                     SPIVLLAGDSLASVTLNGIAAGNDTIRATATGYVAGAIGVPATVNLISLPTTLTVPFG
                     GTTTIGVTLAAPAPAGGVVVTLVSSDTTKVRILTPTVSFAQGVQSQNGQIAGVALGTA
                     SVTASNPNYAPATSSVTTAANLNIVNAAATIYPAFPDSQTVQFMSSGTPIAAPAGGIV
                     VALAAADSTCVSVPASVTITGGLSTATFPVSYAGKAATPCTSWVTASASGVGPDSVLM
                     TVGNPPTIASGTLDLGAGLQTQVYVSLQTATQGNLNMTIRPLKVGVARFAPNLTTLGV
                     DTLVVPLPNGTTSLYATAAGVDTVTNDSTFVEISIPGYAVDTALVRVRTPGVILYGVP
                     TSTTSLSAPSAIYAYVGVPYVGNSGIQSYQGPRVGHAPLTATFHVTPASVAELTDSTG
                     VLDTVRTAVLPVAANLYYTPTSIASGGVGYHPVGAGSSTTTVSIPGFVSLPSTTGTVV
                     SAPTISAGAPQVGAGLQTSVYVSFAAPVPANDTLTVRSLKPTVLLVADSVSHVGADST
                     RMPLVSGATSTYIYVAGMDTVVSDSSQFVLSVPSFKPDTVWGYVRKAGLVLLGLPTST
                     TTLTAKSQFYAGIGLPYVGNTGIYTVQPIRAGGSPDTVTFHTAQP"
BASE COUNT         1887 a         4643 c         4780 g         2090 t
ORIGIN      
        1 gcggcgacct actgggtggc cgccgatggg cggatgatcg gcgcgacgat gaacgaggac
       61 gcgtcgatga ccatcaccct cgaccaggcc ccggcgccga tccccgtcaa gtcgaccaac
      121 atcgtgacga tctcggccat caagtgaggg cgctcctcat cggcgggctg gccctcctgg
      181 ccgcctgcag cagcaacgcc ggcgcaggga ccgacgtggc gccgcgtgac ggccatgggc
      241 gggcgcgccc gatggcgccg gtgacgatcc gaccggcgcc gccggcgcgc tacgcgttct
      301 accggcagga cagcgtcacc acccggctcc ccaacggcgc cgagtcgccc caggtggtgg
      361 ggcgcacgct gctcctcacc gtgtcgatca cgccgccggc ggcgggcacc acgggggacg
      421 cgcggatctc gatcgcactc gactccatcg cgatcgatcc gggcacgccg gagatggcgc
      481 gggcgccgtt cgtgggtgtc gccgggacga cgtggtcggg gaccgtggac ggcaccggcc
      541 gcatctcggg ccttgcgccc gaccggccat cgaccggcgc ggaagcggtg acccccgacc
      601 tccagcgctg gctcccggtg ctccccgcgg ccggagcgcg ccctggcgac gcgtggaccg
      661 acacggtcgc atcggtctac ccgttctcga acctcaacct gcaggtggcg gacacgctca
      721 tcgcggcgtg gaccgcgaag gccggcgtcg cgccacggac cctggagatc cgggacagcg
      781 ggacgatcgc ccggaagggc accagcaaca tcgtgacgct gatgggcggc ggcacccgga
      841 cggcgcagta caccctcggc gccgacggca ccctcgaggc ggcgagcggc gtcgactcgg
      901 tggtcatgaa gatctcggtg ctggcggtgg gccagtcggt caccgtccgc cagttggggc
      961 gctacacggt gacccggcga ccgtgaccgg atgacgctgt ccttcaggaa cctcctccgg
     1021 ggggtggccg gactggcggg agccttcgtg gtgtggctct tcgcggtctg gccgcccccc
     1081 gcctggtatc ggagccactg gccggccgag acggcgttcc aggagatgcg ggcgcaggag
     1141 cgcggggacg caggggcgca gggttgcagg ggtgcagggg gagtcgcgca tcaagattcc
     1201 aatcgctcgt tcctgcgtcc ccgcgtctct gcggccctgc gcccctgcgc ccgcctgtac
     1261 caccccgtcc ccctcgactc catcgcaccc tcgatggtcg atgcggcgat ggcgggggag
     1321 gaccagcgct tcctggagca ccacgggatc gactgggtca acctgcgccg cgcgctgggg
     1381 tacgaccgcg acgatttccg ctggagctcg gcccgcgacc ggggcgacct cctccgggcc
     1441 ctgccgcacg cctgggctcg gcgcgaccgg ctccgcgggg cgagcaccat cacccagcag
     1501 ctggccaaga acctgtacct ctcgccctcc cggaacccgc tccggaaggt gaaggaggcg
     1561 gtgaccgcct ggcggctcga ggccgcgctc gacaaggacc ggatcatggc gctctacctc
     1621 aacacggtgg agctgggcca cgaagtctgg ggggtcgacg ccgccagccg gctctatttc
     1681 aagcgtcccg cccggcagtt gacgctcgca gaatcggcgg agctggcggg ggagctgccg
     1741 ttcccgctcc ggtcgaaccc gggctaccga ccggcgcgca tgttccaccg ccagcagctc
     1801 atcctggcgc acctgcgcgg ggagccggtg gagatccctc ccgactccgg cctcgcggac
     1861 gtggcggaca cgctcgtgcc ggccccgacg gtcgaatcgc tgccgccgat gccagaggag
     1921 tcgctgccac aactactaaa gaccgacagt gggggaacgg ctcaggtgga cagtggtgcg
     1981 gtgggacagc gcgacagcgt gaaggacgct ccgccgcgca gctgaacgac cacacggcta
     2041 gcccaccctc tgcaacgaca gacgctccgg cgaagattgc cggagcgtct gaacgtctgt
     2101 tcccggatcg ggcgggcgaa ggggccgccc cgccggatca ctgccggcag ggctgcttcg
     2161 gatcctggat ggggccgtcc accttgagcc gctcggggcg atcggccacc gcgagcgcga
     2221 tgtcgcggac gaagcgggcc accttggcgc cgtgctcgta gtccacgtat tgcggctcgt
     2281 cggtcagctg gtggtagtcg atggcgtacc ccagcgagaa gtaggtgatc gggatcccgt
     2341 accgggcgta catgaagtgg tcgctccggc agtagcggtt gagcgggttg ccgtgggcgt
     2401 cccaggtgta gttgatggcc atcggctcgc tgcggtgggt gttgagcgag tcgatcagct
     2461 tgccgaggtc ggtggagagc cggtaggagc cgatggtctg caccgagttc gggctcccgt
     2521 ccatgtcggt ggggcgtccc ttgccgacca tgtccatgtt gaggtcgacc acgatggagt
     2581 cgcgcggcac ggtggggtgg tcggtgtacc actgggagcc gaggagcccc ttttcctccc
     2641 ccgtgtggga gacgaacagg atcgaccgtg ccggacggtc gggacggtgc gccaagtcct
     2701 cggcgatctc gagaagggcc acggtgccgg agccgtcgtc atcggcgccg ttgtagatcg
     2761 agtccatccg caccacgccg ccgcggcggg cccgggcaga gtcgaggagc gcgtggatcc
     2821 gggcccactg ttccggggtc ggaggagcgg ggcggtcgtt ggcgccccac ggccgcatga
     2881 cccggttgta cgcgcggatc gaatcgtgat cggcgggccg gcccattccg acgtggtcgt
     2941 tgtgggcgct gatcaccaca tactcgccgt tgcgcctggg gtcgctcccc ttgaggaggg
     3001 cgatcacgtt ccgcgccggg tacgccgtgg gaccgacggc gtagtgcacc gtggcggtga
     3061 cggcggctcc ggcggccccc gccgaaaggc tgtcgaccgg atgcccgaag agccgcgcaa
     3121 cggccgatgc gctgatgaac gcggccagcg gtccctcggc ggggcggtcg ctctgcagga
     3181 actcccgggc gtggtcgaag ttggaggcga tgaacggcat cacggaatcg gggatcacca
     3241 cggccaccgc ggcggcgcct gcggcacgcg gctcgctcgc gccccagaac ccgagcgagc
     3301 ggccatcacc ggggagccgg aagatcgcga ccttcccctt gaggccggcg gcatcggggg
     3361 tcccggcggg atcgccccag gtgccggcga agacggacgg agcgtcggtg acgttgagct
     3421 cgggacggaa tggcgccgtg ccgaggggtg ggagcggcac gatgtcgctc caggggcgga
     3481 tcgggccggc gctcgagcgg aaggcgagag ttgaatcgaa gaggccgcgc tcgaggccga
     3541 tggcctggaa gaaggtgccg ccctcgccca tcggcgtgag gccgtccctg gcgaactccg
     3601 cggcgatgta ggtggtgccc ttgaagttcc cgagctcgcc gctctcccgt ccctgcatcg
     3661 agtcgtcggc gaagcggtag agccgcgtca tcatgtcggc cgcggtgaca tcgggcgtgg
     3721 tgggacgggg tgccagggtg cggaagggga gctcgtcggg ccacgcgggc tgcaccccgg
     3781 ccgtcgcggc cggggacggt gccgacgtct ccgccggggg cgtcgtggtg acgcgggggg
     3841 tgcagccggc gcccagcagg gcggcgaggg caaggagggt cgagcgggac atctcaggtc
     3901 tccggggatg cgaagggccc gccacccgac ggcgggcccc ggcggaaagt tgcgggcggt
     3961 cagtcgacca ggcggagcgg catcaccagg gtcatgtagc tcgcgggatc gtcccagccg
     4021 accggctcgc aggtggccgc gcgctccggc cccttgaacg tgaggcggat ctcgtcggtg
     4081 ggcatgtact tgaagatctc gagcaggtag gtggcgttga agccgatgtc gagcgcctcg
     4141 ccgtcgtagg ccacggccac ctcttcctgc gcctcgccca ggtcgggggt ctgcaccgag
     4201 agcttgcagg cgccgttgct gagcgtcatc cggatccggt gggtctggtc gctggccacg
     4261 atgctcatcc gccggagcgc cgccgtgagc gaggcgcggt cggcggtggc gaccttgtcg
     4321 ttctcccggg ggatgacctg ctcgtagttc gggtatggcc cctcgatgag ccgggtgaag
     4381 acctgggtcg tggcggagcg gaagccgagg tggttctcgc tcttggcgat ctcgatctcc
     4441 tcgtcggccg cgaagagccg gcggatctgg tcgagcgcct tgggcgggac gatcagcttg
     4501 gcctcttcgg cgaccccggt cttgagcggg atgtccatct tggccagccg gtgcccgttg
     4561 gtggccacca tccgcatccg gtcggggcgg agctcccagt ggacgccgtt gaggatgggg
     4621 cggctctcct cggtgctcgc cgcgaaggcg acgtgcgaga tgagcttctg gaggtcgccg
     4681 gacgtgcagc gccacccgcc atcgaacttg accgccggga acgccgggta ttcctcgcgc
     4741 gggaggccca ggagcttgaa cttggcccgg cccgactcga tgctgacgcg ctgctcaccc
     4801 gagctggtga cacggatcga cccgctcggc agctcccgga cgatctcctg gagcttcttg
     4861 gccggcaggg tgatggcccc ttcctggtcc accgacgccg ggatcagcgt gctcaccgcg
     4921 atgtcgaggt ccgtccccga cagcctgagc ccgtccttcg aggcctcgac caggatattg
     4981 gagagcaccg gcagggtcgt cttggcgggg atgctcgccg cgacgctggt gaggccctga
     5041 tggagctgtt cgcgcgtgat ggtgaacttc atgcgttccc cgctggaggc cagaacaacc
     5101 tacttctaac cgtttataag taactagcag tagtagtagt acatctggaa atgtggacgc
     5161 ccgccgtaag ccgctctcct gcaagcgcct gaccccgaga ccgactgtcg agtgactgtc
     5221 ggggttctgg atgaaatgtg ggctcgtcct tcccggaccg caaagcttcc ccatccgccc
     5281 acgggccctg tccacagtgt ggacggcacc gtggagagcc cggtcagttg gtgaccagtt
     5341 cctggcgcgc ggcctcgatc cggtcgcgga aggagcggtc gcgctgcatc tggcgctcga
     5401 ccttctcgac gctgtggatg acggtcgagt ggtcgcggcc cccgaaggcc tggccgatct
     5461 cgacgagcgg catctcgagc aggtggcgcg ccaggtacat cgcgatctgg cggggcacgg
     5521 tcagggtctt gatccgggtc ttggagcgga gcccctcggt ggtgacgccc caccggcggg
     5581 ccaccacgtc ctgcaccttg tcgatcgacg gcagggcgcg aatggcggcc gcggcgtcgt
     5641 cggcgtcgcg gatcttgtcg gccagcgcct cccgggccag gtcgatcgag acctcccggt
     5701 gccggagcga ggcgaagagc agcagcttga tgatgcaccc ttcgagctcc cggacgttgg
     5761 agcggacgtg ctcggcgatg aaccgcagga cgtcgtccgg gatggtggtc tcgaggtggt
     5821 cctgttcctg cttcttgcgc aggatggcga tccggtgctc gaggtcgggg tggcccacgt
     5881 cggccaccat cccccactcg aaccgggtca ccaggcgcgc ctcgaggccc tgcagctcct
     5941 tgggcggccg gtcggaggtc agcaccacct gcttcttgtt gtcgtagagg gcgttgaacg
     6001 tatggaagaa ctcctcctgg gtctggacct tgccctggag gaagtgcacg tcgtcgacga
     6061 ggaggaggtc gacgccgttg cggtaccggc gccggaactg ctccatcgtc cccttgccga
     6121 tcccctcgat cacctcgttc atgaactgct cggcgccgag gtagagcatc cgggtctcgg
     6181 ggttgcgctg gacgacggcg tgggccaccg cctgcatcag gtgggtcttg ccgagtccgg
     6241 tggcgccgta gatgaagagc gggttgtagg tcttgccggg ggattccgac accgcgtggg
     6301 cggcggcggc ggcgagttcg ttggacttgc cgatgacgaa cgtatcgaag gtgtagcgtt
     6361 cgttaagtgg ttgctgtgat tgattttgcg agccggggac ctcgccggcg gggcgggcct
     6421 gctggacgaa gaagtccatc tggggccgct ggaggcgcgc ttcctggacc cggaagacga
     6481 ccgacgtggg gcgaccgacg accccttcgg cgaggcggcc gagcaccgag gcgtactttt
     6541 cctgattcca ctggacggcg aactggtccg gggcggcgac cgtgagctgg ccatcctcca
     6601 gcaccaccgc ttcggtgggc tggagccacg acctcatcgc ttccgtcggg agttggcgct
     6661 gggcctcgtc gagaatgcgc gaccaagcgt ctttggcgga cagctgcggc atcggttctc
     6721 agggagtgtt ccgaaagcgg atcagcaagg gcgggagaga atagggccgg aatgtggaaa
     6781 agtcaattga cggaaaagtg ccgggtttgt cggaagagag gtcggtgccg cgcagcgaac
     6841 gacgagtggg acacccggtc cccgcgaccc gccacccgcc cgtgaccttc cactcgtcac
     6901 tcgtcacccg ccggcccctt gccccaactc cctgacccat ctagctttcc gggctcactg
     6961 gaggacagga aagagccatg aagccgtcat atcgtccgcg caaccggcgc cggatcaaca
     7021 agcatggatt ccgcgcgcgg atggcgaccc gctggggccg cgccactttg agccgtcggc
     7081 gcaagaaggg tcgcaggcag ttgacggtga agttgccgtc gaagcacggg ggccgctgac
     7141 ggcggaacgt ctgctgcggg ccgaccggct cgcgcgggcg tcggagttgc gccgctgtct
     7201 ggcccatggg cgccggcggc gcggggcgca cctcgagatg atctggctgg acaattcatc
     7261 gggccacccg cggatgggga tgatcgttcc ccgttttcag ttctccgcgg tggcccgaaa
     7321 tcgtttgcgg cggcggatcc gcgaagtgtg gcggcgggag gtccggccgg cgcagccggg
     7381 gtgggacctg gtggtgcggg cgcggcggga ggcgtatgcg gcgccattcg ctgcgctccg
     7441 cggggagctc ctggcctggc gggaggcggt gtgcgggtag ggggcgcagg ggcgcagggg
     7501 cggacggacg gatcgggcgc gccggcgaag tcgtgcgacc atccgaccat gcgtccgtcc
     7561 gtctccctgc ggaactggcc ccgcctggcc ggctggtacc tgatccgcgg ctaccagcgg
     7621 ttcatttccc cgatgttccc gccgtcgtgc cggttcacgc cgtcgtgctc gcagtacgcg
     7681 ctcgaggcgg tgacccgcta cggactcctc cgcgggacct ggctcggtgg tcggcgcctc
     7741 atgcgctgcc acccgttcca ccccggcggt ttcgaccccg ttccctgacg cgagaatcct
     7801 gaagatggat cgtcggatca tagcggccgt gttcctgatg atgctggtgg cggtggtgcc
     7861 gtcactgatc ttcaaggcgc cacccaaggc cgtcacctcg caggtggcgg acagcgcggc
     7921 ggtgggtcag cgggatagtg gcacggtggg acggggtgcg gctctgcccc agcgcccctc
     7981 cgccccagcg cccctgcgtc cgtccgcccc agcgcccctg cgcaccgccg cgcccgccca
     8041 cgatgacagc gtcgtcgtca cgtcgggtct ctaccgctac gccttcagca cccgcggcgc
     8101 gcggctcatc tcggcgacgt tgcccaagta tccctcgatg gccacgaccg ccgctcccgg
     8161 caccccggtc gagctggtgc ggccgggcgg gggactgctg gcgctgacac tggtgtcggg
     8221 gcgggacacc gtgccgctct ccgactggac attcacgccc tcggcccgga gcgtcgcggt
     8281 cccggccgac gggacggcgc ccctcacgtt caccgcttcg caggggggcg tcaccgtcac
     8341 cctcggcttc accttccgct cggccgatta ccgcttcgac gtggcgggga aggtcgaggg
     8401 cctcggcccc accggcgggt tcctcctggt ggggctgggc ggcgggctcc cgaacaccga
     8461 ggccgacacg ctcgccaacg ggcgggccca cgcgctggtg gtcaagcccg tggggaagag
     8521 caccaacctc atcccgttct ccaagatcga tcccggcacc tcgcgcgaag tgccgggacc
     8581 gttccgctgg gtggccagca agtcggagta cttcgtggtc gggctctttg ccaccgacac
     8641 cgcgtcgccg tggacagcga cgcgggcggt ggtgcccgcc ggggtcggga agtacgccgg
     8701 cgtcgcggcg ctcgaggtgt cgcagccggt ggcgggtccg gggacgttcg gctaccagct
     8761 ctatgcgggc ccgatggagt acaaccgcct ctcggccatc gggcaggact tcgatgacgt
     8821 gaacccgtac gggatcgtcg gcttccgcgc cgtcatccgg ccgttcgcgc tcttcttccg
     8881 ccaggtgttc gtgtggatgc acacccacct gggcatctcg tacggttttg ccatcatcct
     8941 gctggggatg ctgatccggc tcgtcctgtg gccgctcaac cagaaggcga tgcgctccgc
     9001 gatggcgatg caggccatcc agccgcacat gaaggcgctg caggagcggc acaagagcga
     9061 cccggcgcgg ctgcagcagg agatggtgaa gctctacaag gagcacaacg ccaacccgct
     9121 cggcggctgc ctgccgatgc tgatcccgat gccgatcctc ttcgcgctgt tcttcgtgtt
     9181 cgagagcacc atcgagatgc gcggggtgcc gttctggtgg ttcaccgacc tgtcgcaacc
     9241 cgacccgcac cacatcatcc cgttcctcac cggcctctcg atgctgctgc tcagctggat
     9301 cggccagcgg tcgatcccgc ccaatccgca ggccaagatg atgatgtgga tggcgccggt
     9361 gatgttcacc ttcttcggtt tccggttcgc ctcggggctc aacctctatt acgtggtgag
     9421 caacctggcg agcgtccccc agcagtggct cctgtcggag gagcgccgga agcggaaccc
     9481 gcccgccccg ccggcgccgg cacccggcgg gcgctgagcc ggggccgggg cggcggacgg
     9541 tgcgggggcg gcgcatgcgc cgcccctgct gtttgggggg tgtgatcccc gtcaccgatc
     9601 cggtgcgacc ggctccgtag ccgaccggag ctccgggcgg taagtttgcg ccctatgcct
     9661 tccagcgtcc tgctggcctc ccgcgcccgt gcgctcggcc gccagctcct cgtcgccgcg
     9721 tccgcattgc tgctcgtcac ctgcaccgac aacccgaccg gtccgacggt cgggtcgacg
     9781 ctgcggctgg cggtctcgcc ccggttcaag gcggggacgt tcctgagtcc ggcgctcccg
     9841 gtcgaccagg tgcggctggt ggtgtatcac cagccggcgt gcgactgcgc ggcggattcg
     9901 atcgcggggg cgacggtgcc gttcgcgctc accgacaccg cggtgtcgct cagcgtgccg
     9961 gtcaggctga cgcagccggc ggagtcgctc acggtccagt tctacctgat gggcggcggg
    10021 gtgcagctct tcttcgggtc gcaggcggtg gtgctgacca gtggctccac gccggtgatc
    10081 ccgccggtgc tcctgacgta cgtcggtccg ggtgcggggc tcgactcgct cctcatcacg
    10141 ccccgggatt ccgtcctcac cttcggcgac tcgctcacct tcggcgtggc cgccttccag
    10201 gtcggcgcgc cggtctcggc gttctacgtc ggctggaagt cgtccctggc caccgccaag
    10261 atcctccccg acgggaagat ccacgctccc ggcgcgcgtt cgaagacgac cgtcacggtg
    10321 caagccccga acggaaaggc cgacagcacc acgctgacct tcgtgccgaa gccggtgacg
    10381 ctcttcaagg tgagcggcga cacccagtcg gcgccgagac tcgacacgct gccgctgccg
    10441 ctccgggtac gggtcaaggc ggcggacagc ctgggcgtgg gcggggtgcg ggtggtgttc
    10501 catccgctgg gctttgcggg cgccgccgtc cccgacaccg tgtacaccga ctccctcggc
    10561 tttgcccagg tgcgggcggt gctgagcgac agcgtcggga cccagcagtg gcaggccacc
    10621 gccgccgggc tcacggcggt gacgttctcg gccaactcgc aggtcgtggc cggaccgccg
    10681 tatcgggtga agctcgtcac ccagccgtcg gacaccaccg ccagcggcgc cttgatcccc
    10741 gtgcagccgg tgctgcaggt cgaggattcc gtgggcgtcc cggtgcacgt ggccggcgtc
    10801 gtcgtgacgg cgcagtcgct gacctacctg accggcgcgc cacagcccaa gcggccgctg
    10861 ggcggcgggc tcaagccgtc gtcgacccgc gccagcgcgc cgtacaagtc ggccgacacc
    10921 ttcggacaga tctccgattc caccgacgcg accggcaagg tggccttcac cgggctccgg
    10981 atggtcggct acggcgcgga acgcgtcgtg ttcaacgcgc ccgcgctcct cgccgacacg
    11041 tcggtggcca tgtgggtcac cgccggcgcg cccaagaatc tcgtcaaggg ctctgccgac
    11101 tcgctcaccg cctacgtcga cagcctggtg cccgtggcgc cggccgtcta cgtgtatgac
    11161 tcgacctaca atccggtgcc gaacgtcgcc gtcgtgttcc aggtgaccgc cgggggcggc
    11221 accatcccga agccggtcga caccgtcttt accgacacca ccggcctcgc cactctcgcg
    11281 tcgtggaagc tgggcccgaa cccgggcacc aacaccgtcc aggccaccgt ggcgggggcg
    11341 gggagcctca cgttcaccgc gttcgcgcag ccgccggtgc cgacgctgct cctgcaactc
    11401 cagggcacgt cggtggtggg cgtcggtcgg acggccacgc tccaggtgaa gctcagctcc
    11461 cccgccaccg cgaccgacac gctctacgtg acgagcgaca gcacccagac catcaccgtg
    11521 agcggctcgc cgatcgtgct tttggccggc gactccctgg cgagcgtgac gctcaacggc
    11581 atcgccgccg ggaacgacac catccgcgcc acggccaccg ggtacgtggc cggcgccatc
    11641 ggcgtgccgg cgaccgtgaa cctgatctcg ctgccgacca cgctcaccgt gcccttcggc
    11701 ggcacgacga cgatcggcgt gaccctcgcc gcgccggcac ccgcgggcgg cgttgtggtg
    11761 acgctcgtga gcagcgacac caccaaggtc cggatcctca cgccgacggt gtcgttcgcg
    11821 cagggggtgc agagccagaa cgggcagatc gccggcgtgg cgctcggcac cgccagcgtc
    11881 accgcgtcga acccgaacta cgccccggcg acctcgagcg tcacgaccgc ggccaacctc
    11941 aacatcgtca acgccgcggc gacgatctac ccggcgttcc ccgactcgca gaccgtgcag
    12001 ttcatgagtt ccgggacgcc gatcgcggcg ccggcggggg ggatcgtcgt ggcgctcgcc
    12061 gccgccgact ccacctgcgt gagcgtcccc gcctcggtga ccatcaccgg cgggctctcg
    12121 accgccacct tcccggtcag ctacgccggc aaggccgcga cgccctgcac cagctgggtg
    12181 accgcgtccg cgagcggcgt ggggcctgac agcgtgctca tgaccgtggg caacccgccc
    12241 accatcgcga gcgggaccct cgacctcggc gccgggctgc agacgcaggt gtacgtgtcg
    12301 ctgcagaccg cgacccaggg caacctcaac atgaccatcc ggccgctcaa ggtcggcgtg
    12361 gcccgatttg ccccgaacct gacgacgctc ggggtcgaca ccctggtggt cccgctcccg
    12421 aacgggacga cgtccctcta cgccaccgcg gccggcgtcg atacggtgac caacgacagc
    12481 accttcgtcg agatctcgat ccccgggtac gcggtcgaca ccgcgctggt gcgggtgcgc
    12541 acgccgggcg tgatcctcta cggcgtgccg acgagcacca cctcgctctc ggcgccgagc
    12601 gccatctacg cctacgtcgg ggtgccgtac gtcggcaatt ccggcatcca gagctatcag
    12661 gggccgcgcg tgggccacgc gccgctcacg gccacgttcc acgtgacgcc ggcgtcggtg
    12721 gcggagctca ccgactcgac gggcgtgctc gacaccgtgc ggacggccgt gctgccggtg
    12781 gcggccaacc tctactacac gccgacctcc atcgcgagcg gcggcgtggg gtaccacccg
    12841 gtgggggccg gatcgtcgac cacgacggtg agcatccccg gcttcgtgag cctgccctcc
    12901 accaccggca ccgtggtctc ggccccgacg atcagcgccg gcgcgccgca ggtcggggcc
    12961 gggctgcaga cgtcggtgta cgtctcgttc gccgcgccgg taccggccaa cgacaccctg
    13021 accgtccgga gcctcaagcc gacggtgctc ctggtggccg acagcgtgag ccacgtcggc
    13081 gccgattcga ccaggatgcc gctcgtcagc ggcgccacct cgacctacat ctacgtggcc
    13141 gggatggaca cggtggtgag cgacagcagc cagttcgtgc tgtcggtccc gagcttcaag
    13201 cccgacaccg tctgggggta cgtgcggaag gcggggctcg tgctcctggg gctcccgacc
    13261 agcaccacca cgctcacggc caagagccag ttctacgccg ggatcgggct gccgtacgtc
    13321 ggcaacaccg ggatctacac cgtccagccg atccgggccg gcgggtcgcc ggacacggtg
    13381 acgttccaca ccgcccagcc
//
LOCUS       DASUAP010000207         6063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_809915, whole genome shotgun sequence.
ACCESSION   DASUAP010000207 DASUAP010000000
VERSION     DASUAP010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809915"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..947
                     /gene="nusA"
                     /locus_tag="VFU45_05310"
                     /pseudo
     CDS             261..947
                     /gene="nusA"
                     /locus_tag="VFU45_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
     gene            969..1244
                     /locus_tag="VFU45_05315"
     CDS             969..1244
                     /locus_tag="VFU45_05315"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU5358512.1"
                     /translation="MERGGEGPLFAPLPPEEAAASRVPLAELEGLAPEIVAILAGAGY
                     NTLDDVFDLDREELAAVPGLSSAQVDALMAFLSELADEDGEEPGAAR"
     gene            1241..1546
                     /locus_tag="VFU45_05320"
     CDS             1241..1546
                     /locus_tag="VFU45_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HEU5358513.1"
                     /translation="MSALLRLLGLGRRSGALVIGADAVHRALQENGVHCVVLAADASP
                     RAREKVVPLATGRGIPLLAGPAAEVLGGQLGKPPVMAVGVRDRALADGLVAAAPDSP"
     gene            1558..4275
                     /gene="infB"
                     /locus_tag="VFU45_05325"
     CDS             1558..4275
                     /gene="infB"
                     /locus_tag="VFU45_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5358514.1"
                     /translation="MVKTRVHDLAAEFGISSEQLLTLLKDMDIFVRNHLSALEGDQVA
                     AVRVRWEREKRKSSEDAQPKRGRRKAKVVEAPTPEPARPARRRRTAAEVAEAEAQAQA
                     ERQEAEREAIELDIELPRIEPAEPVVAQTLEERARAIFKDLPPAPAPEVEADQEPAPV
                     ARRQPVIEPPAAPVENRPRPAAAESGTAPAAPVKAPFIPPRVARPAAGARPAGPRPVF
                     STSTPGSGTRERPAPARPTEERPAVGNTGMGGRKKGKKGRRGYVDQDAVQENIAKTLQ
                     GLKGGSGRKGARRSDEPSYRDIQAGLQKEEREKEKTRIRVNEFISVSELAAAMKIPAT
                     QIVQFAFKELGLMVTVNQRLDFDQIELIASEFGFEAVKEAEYQAEVEQTEAPDSPEDL
                     VPRPPVVTIMGHVDHGKTSLLDHIRKANIVAGEAGGITQHIGAYHVELPSGKEITFLD
                     TPGHQAFTAMRARGAQVTDIVVLVVAADDQVMPQTVEAISHARNAGVPLVVAINKIDL
                     PSANVPKVKQDLLQQNVVLEEFGGKVQSAEISARRGTGVDLLLEKILDEAEILELKAN
                     PTARPQGTVLEATLDPGKGPLATVLVQRGTLKVGDDFICGMFSGRVRALFDERGNPVA
                     EAGPSTPVQVLGFDGVPSAGDSVLTVTDASEARDIAQKRQRLEREAQSRRSQRGTSLE
                     DFSRALKEGAVSTLPIIIKADQGGPAEALADALAQLSTAEVRVDVVHRGVGAITESDV
                     LLARASSAIILGFHVRPDSNARAAAEREGVDVRTYRVIYEAVDDVRKGLEGLLKPEEK
                     ETILGEAEVLQLFKVSKVGTIAGCSVRSGVIPRAAHVRVIRDGVAVYTGVISSLKRFK
                     DDAREVREGQECGIGVENFNDLKVGDTIEAYRMEEVKRTLESAGAAGAS"
     gene            4283..4558
                     /locus_tag="VFU45_05330"
     CDS             4283..4558
                     /locus_tag="VFU45_05330"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEU5358515.1"
                     /translation="MGITVWELHLAGCRSLKDKRSALKPLLNRLRQQCNVSVAETGHQ
                     DRWQHAEVACAAVGSDRRVIEETLRAADRLVEGSDGVRIMDTATVYR"
     gene            4555..4917
                     /gene="rbfA"
                     /locus_tag="VFU45_05335"
     CDS             4555..4917
                     /gene="rbfA"
                     /locus_tag="VFU45_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU5358516.1"
                     /translation="MKGSSRRPEQVGETIRQVVADALMRGEVRDPRIGLVTVTRVEVS
                     GDLSHAKVYVSPQGDDAERARAMEGLESAAGYLRSLVARVLTTRSVPELHLEQDRGLE
                     HAARIEQLLADLRREEQG"
     gene            4932..5765
                     /gene="truB"
                     /locus_tag="VFU45_05340"
     CDS             4932..5765
                     /gene="truB"
                     /locus_tag="VFU45_05340"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU5358517.1"
                     /translation="MDKPAGMTSHDVVASVRRTLGERRAGHTGTLDPFATGLLVVLLG
                     RGTRLARFVEGRDKTYLATARLGVATDTDDVTGTVIGGTDRKVPDAELDAALRQLTGP
                     QEQRPPAYSAKHVEGRRSYQLARRGEAVELPPVDVMVHELEVLERQGDLISFRTRVSA
                     GTYVRALARDLGDLLGTGAHLTALRRERIGRLDLSAATPLAELTGQAPLRPLRDVVPD
                     LPAVTLDADAAADVRHGRPVPCADADAPAVALLAGERLIGIAERTGGVLHPRVMLEDP
                     A"
     gene            5771..>6063
                     /locus_tag="VFU45_05345"
     CDS             5771..>6063
                     /locus_tag="VFU45_05345"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEU5358518.1"
                     /translation="MTVGTFDGVHRGHHAVITEIRRRAAARNLPSVLVTFEPHPLEIV
                     NPAAAPLLLTPGQERLEALATTGVDRVVVLRFDRALAAMEPEQFVREVLRARLG"
BASE COUNT          943 a         1983 c         2326 g          811 t
ORIGIN      
        1 gaaggagctg aactgtgtca tcggcgtccc gatgagccgt tccgttccga tcgtgagcgc
       61 gcgcggttct tcctggcgcc gcctcgcccc cgagcgagct tccgcgcctc ggtggcctcg
      121 gcgagcaacc gagggaacgc cggattccgt cgggtccgct gctcgatgat ctcgtcgacg
      181 aagtccgctg ccacgggtgg aagcctgtag cgccactggt gggacgtcaa cgccatcggc
      241 gctgaagggt ccaggcggcc aagcagcgca tcatccagcg ggtgcgcgag ggcgagcgga
      301 cccgcatccg cgaggagttc gccgaccggg tgggagagct gctgtccggc gagatccagc
      361 agatcgagcg cggcaagctg gtggtcatgc tcgccaagtt ccgcgaggcg gaggcgatca
      421 tgccctaccg cgagcagaac caccgggagc acttccacca gggcgacacg atgcgggcgg
      481 tgctgaagcg cctcgaggag acgcccaagg gcccccgcct catcctgagc cgcgccgacc
      541 cgctcttcgt caaggcgctc ttcaagctcg aggtccccga gatccagcag caggtggtcg
      601 agatccgcgc catcgcccgg gaggtgggca gccgcaccaa ggtcgcggtc atctcgcggg
      661 atgacgcgat cgatccggtg ggtgcgtgcg tgggactcaa gggctcccgg gtgcaggcgg
      721 tggtgaacga gctcggcggc gagcgcatcg acatcgtgcc gtggtccccc gacccggaac
      781 ggttcgcgcg gctggcgctg gctccggccc gggtggccaa ggtcttcgcc gacgggccca
      841 gcaagacgat ccaggcggtg gtggacgagg accagctgtc gctggcgatc gggcggaacg
      901 gccagaatgt ccggctcgcc tccgagctca ccggatggaa gatcgatctc tactccagcc
      961 gcgagtggat ggagcggggc ggcgagggcc cgctgttcgc cccgctgccg cccgaggaag
     1021 cggcggcatc acgggtgccg ctcgcggaac tcgagggcct ggcgcccgag atcgtcgcca
     1081 tcctcgccgg tgccggatac aacacgctcg atgacgtctt cgacctggac cgggaggagc
     1141 ttgccgcagt gcccggcctc tcgtcggccc aggtggatgc gctgatggca ttcctctccg
     1201 agctcgcgga cgaggacggc gaggagccgg gcgcggcgcg ttgagcgcgc tgctccgcct
     1261 cctcgggctc gggcggcggt cgggcgcgct ggtgatcggg gccgacgcgg tgcaccgggc
     1321 cctgcaggag aacggcgtgc attgcgtggt gcttgcggcg gatgcgagcc cgcgggcgcg
     1381 ggagaaggtg gtaccgctgg cgacggggcg gggtattccg ctgctcgcgg gtccggctgc
     1441 cgaggtgctc ggcggccagc tgggaaaacc gccggtgatg gcggtgggtg tccgggatcg
     1501 tgcattggcc gatggcctgg tggccgcggc accggacagc ccttgaggga ggcgtgagtg
     1561 gtcaagactc gggtacatga tctcgcggcg gaattcggca tttcctcgga gcagctgctg
     1621 accctgctca aggacatgga catcttcgtc cgcaaccacc tgtcggccct cgagggcgac
     1681 caggtggcgg cggtgcgggt gcgctgggag cgcgagaagc gcaagagctc cgaggacgcg
     1741 cagcccaagc gcggccggcg caaggccaag gtggtcgagg caccgacccc cgagccggcc
     1801 cggccggcgc gccgccgtcg cacggcggcg gaggtcgccg aggcggaagc ccaggcccag
     1861 gcggaacgcc aggaagccga gcgtgaggcg atcgagctgg acatcgagct tccccgcatc
     1921 gagccggccg agccggtcgt ggcccagacg ctggaagagc gtgcccgcgc catcttcaag
     1981 gaccttcccc cggcccccgc gcccgaggtt gaggccgacc aggagccggc acctgtcgcc
     2041 cggcggcagc ccgtgatcga gccgcctgcg gctccggtcg agaatcgtcc tcgcccggca
     2101 gcggccgaga gtggcaccgc accggcggca ccggtcaagg ctccattcat tccgcccagg
     2161 gttgcccggc ccgccgcggg cgcacgtccc gcgggcccgc gtccggtgtt ctcgaccagc
     2221 acgccgggat cgggtacccg cgagcggccg gctccggcgc gcccgacgga ggagcgccct
     2281 gccgtcggca acaccggcat gggcggccgg aagaagggca agaagggccg ccgcggctat
     2341 gtggatcagg atgcggtgca ggagaacatc gcgaagacgc tgcagggcct gaagggcggc
     2401 agcggccgca agggcgcgcg gcgatcggac gaacccagct accgcgacat ccaggcgggc
     2461 ctgcagaagg aggagcggga aaaggagaag acgcggatcc gggtcaacga gttcatctcg
     2521 gtgagcgagc tcgctgccgc catgaagatc ccggccaccc agatcgtcca gttcgccttc
     2581 aaggagctgg gcctcatggt gaccgtgaac cagcggctcg acttcgacca gatcgagctg
     2641 atcgcgtcgg aattcgggtt cgaggcggtg aaggaggccg aataccaggc ggaggtggag
     2701 cagaccgagg ccccggacag cccggaagac ctggtcccgc ggccgccggt ggtgaccatc
     2761 atggggcacg tggaccacgg caagacgtcg ctgctggacc acatccggaa ggccaacatc
     2821 gtggccggcg aggcgggcgg catcacgcag cacatcggcg cctaccacgt cgaactgccc
     2881 agcggcaagg aaatcacctt cctcgacacg ccgggccacc aggcgttcac cgccatgcgg
     2941 gcccgcggcg cgcaggtgac ggacatcgtc gtgctggtgg tcgcggcgga tgaccaggtc
     3001 atgccgcaga cggtggaggc catcagccac gcccggaacg cgggcgtgcc gctggtcgtc
     3061 gcgatcaaca agatcgatct cccgtcggcc aacgtgccga aggtcaagca ggacctgctg
     3121 cagcagaacg tggtgctgga ggagttcggc gggaaggtgc agagcgccga gatctcggcc
     3181 aggaggggga cgggggtgga cctgctcctc gagaagatcc tggacgaggc ggagatcctc
     3241 gagctcaagg cgaacccgac ggcgcgcccg cagggcaccg tcctggaggc cacgctggac
     3301 cccggcaagg ggccgctcgc cacggtgctg gtgcagcgcg ggaccctcaa ggtgggcgac
     3361 gatttcatct gcggcatgtt cagcggccgg gtgcgggcgc tcttcgatga gcggggcaac
     3421 ccggtcgccg aggccggccc cagcacgccg gtgcaggtgc tcggcttcga cggcgtgccg
     3481 tcggcgggtg acagcgtcct cacggtgacc gacgccagcg aggcgcggga catcgcgcag
     3541 aagcgccagc ggctggagcg cgaggcgcag agccgccgga gccagcgcgg caccagcctc
     3601 gaggacttca gccgcgcgct caaggaaggc gccgtctcca ccctgccgat catcatcaag
     3661 gcggaccagg gcggcccggc cgaagcgctg gccgacgccc tggcgcagct cagtaccgcc
     3721 gaggtgcggg tggacgtcgt gcaccgcggc gtcggcgcca tcaccgagag cgacgtgctg
     3781 ctcgcgcgcg cgtccagcgc gatcatcctc ggcttccatg tgcggcccga ctcgaacgcc
     3841 cgcgccgcgg ccgagcgcga gggcgtggac gtgcgcacct accgggtcat ctacgaggcg
     3901 gtggacgatg tccggaaggg gctcgagggc ctgctcaagc ccgaggagaa ggaaaccatc
     3961 ctgggcgagg ccgaggtgct gcagctcttc aaggtgagca aggtgggcac cattgccggc
     4021 tgttcggtgc ggagcggggt gatcccgcgg gcggcccatg tgcgcgtcat ccgggatggc
     4081 gtcgcggtct acaccggcgt catttcgagc ctcaagcgct tcaaggacga tgcgcgcgag
     4141 gtgcgcgaag gacaggagtg cggcatcggg gtcgagaact tcaacgatct caaggtgggc
     4201 gacaccatcg aggcgtaccg catggaagag gtgaagcgga ccctcgagtc ggcgggagcc
     4261 gccggggcat cctgaggtgc aggtgggcat cacggtctgg gaactgcacc tggccgggtg
     4321 ccggtcgctc aaggacaagc ggagcgcgct gaagccgctg ctgaaccggc tccgccagca
     4381 gtgtaacgta tcggtcgccg agacgggcca tcaggaccgc tggcagcatg cggaggtcgc
     4441 gtgcgccgcg gtgggctccg accggcgggt catcgaggaa accctgcgcg cggcggaccg
     4501 gctggtcgag ggttccgacg gcgtgcgcat catggatacg gcgacggtat atcggtgaag
     4561 ggttcatcgc ggcgcccgga gcaggtgggc gagaccatcc ggcaggtggt ggcggacgcg
     4621 ctgatgcggg gggaagtccg cgacccgcgc atcgggctcg tcacggtcac ccgggtcgag
     4681 gtctcggggg atctctcgca cgccaaggtg tatgtcagcc cccagggtga tgacgccgag
     4741 cgcgcccggg cgatggaagg gctggagagt gccgccggct atctccggag cctcgtcgcc
     4801 agggtgctga cgacgcggtc cgtgccggag cttcatctcg aacaggaccg gggcctggag
     4861 cacgccgccc ggatcgagca gctgctggcg gatctccgcc gtgaggagca gggctgaccg
     4921 gcgcactgct gatcgacaag ccggcgggaa tgacgtcgca cgatgtcgtg gcgtcggtcc
     4981 ggcggacgct gggagaacgc cgggccgggc acacgggaac gctggatccg ttcgccaccg
     5041 ggctgctcgt ggttctgctg ggccgcggga cgcggctggc ccggttcgtg gagggccggg
     5101 acaagaccta cctggccacc gcgcgactgg gcgtcgccac cgatacggac gacgtgaccg
     5161 gtacggtcat cggcggcacg gaccggaagg tgcccgatgc ggaactcgat gcggcgctcc
     5221 ggcagctgac cggtccgcaa gagcagcggc ctccggccta ttcggccaag catgtggagg
     5281 gacgccggag ctaccagctg gcgcggcggg gagaggccgt ggagctcccg ccggtggacg
     5341 tgatggtgca cgagctggag gtgctggagc ggcagggtga cctgatctcg ttccgcacgc
     5401 gggtcagtgc cggtacctat gttcgcgccc tcgcccgcga cctcggcgac ctgctcggca
     5461 ccggcgcgca cctcacggcc ctgaggcggg agcggatcgg gcggctcgat ctctccgccg
     5521 ccacgccgct cgcggaactg acgggccagg ccccgcttcg gccgctgcgg gatgtcgtgc
     5581 cggatcttcc ggccgtcacg ctggatgcgg atgccgccgc tgatgtccgg catggccggc
     5641 cggtgccctg cgcggatgct gatgcgccgg cggtggcatt gctggccggg gagcggctga
     5701 tcggaattgc ggaacggacc ggcggcgtgc tgcatccgcg ggtgatgctc gaggatccgg
     5761 catgagcgtg gtgaccgtcg gaaccttcga cggggtgcat cgcgggcatc acgccgtgat
     5821 caccgagatc cggcggcggg cggcggcgcg gaacctcccc agcgtcctgg tcacgttcga
     5881 gccgcacccg ctcgagatcg tgaatccggc ggcggcgccg ctgctgctca cgccgggcca
     5941 ggaacggctg gaggcgctcg ccacgaccgg ggtggaccgg gtggtggtgc tccgcttcga
     6001 ccgggcgctg gcggcgatgg agccggagca gttcgtgcgc gaggtgctgc gcgcccgcct
     6061 ggg
//
LOCUS       DASUAP010000208        11024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_755147, whole genome shotgun sequence.
ACCESSION   DASUAP010000208 DASUAP010000000
VERSION     DASUAP010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755147"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..474)
                     /locus_tag="VFU45_05350"
     CDS             complement(37..474)
                     /locus_tag="VFU45_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358519.1"
                     /translation="MSFRLRIRRQRAVQGLGRITRRFRVHGNVYFGGDTLTIEWTGTM
                     RVETRQLFEAGNETVTVPLEALDVAASDLRSARLRGGWILPFIELEERTLAALDPVPS
                     ENHGLLRLWISRGDRERAARMVAAVQAAISDASATPAEGVTAT"
     gene            complement(483..962)
                     /gene="msrB"
                     /locus_tag="VFU45_05355"
     CDS             complement(483..962)
                     /gene="msrB"
                     /locus_tag="VFU45_05355"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU5358520.1"
                     /translation="MRLKLIDRNGRLVGPIETPRVEKTDAEWRALLTPEEFAVARGKG
                     TERPFCGTLLDNKREGIYTCICCGLPLFSSRGKFDSGTGWPSFFEPVAAENVTTHADV
                     SFGMRRTEILCARCDCHLGHVFEDGPKPTGLRFCVNSESLKFTDQADLAGRADPAAP"
     gene            complement(985..1689)
                     /locus_tag="VFU45_05360"
     CDS             complement(985..1689)
                     /locus_tag="VFU45_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5358521.1"
                     /translation="MPELASGLIYYVVFLFSTTLHEASHAWAALQGGDPTAYHGGQVT
                     LDPIPHIRREPIGMVVLPLLGVIASGWPIGFASAPFDPEWALRHPRRAALMALAGPAS
                     NFLLVVLAGLFLHLGLAHGIFQAPESVSFDHIVEATDPDTMWRAGGFLLSVFFSLNLL
                     LGLFNLLPFPPLDGAGVPPLFLHDDGAHKYQEFVMTHRGLGLIGIVVAWQLIDKLYHP
                     VWLAAVNLIHLAVHYG"
     gene            1776..2309
                     /locus_tag="VFU45_05365"
     CDS             1776..2309
                     /locus_tag="VFU45_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358522.1"
                     /translation="MRAPGQWYRTSAVLLACLGFLVAGGALAQSPPPACTAPEFRQFD
                     FWVGEWSVTDSAGGTPYGTSSVTNEEGGCVIHEHWHSAGGGTGQSFNFYNATRRDWTQ
                     VWVASSGGQLHLVGHLTGGAMVLEQSTVAPNGAPVVQRITWTPKPDGRVRQMWQQSRD
                     GGKTWTVAFDGWYRRKG"
     gene            2444..3856
                     /locus_tag="VFU45_05370"
     CDS             2444..3856
                     /locus_tag="VFU45_05370"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HEU5358523.1"
                     /translation="MLSTPDLSDLLARQRAAFMAAAPPSAATRRADLRRLKAAIVRRR
                     AEFSAAASSDFGHRSPHETLIADLNPTVQSIRYLVRHLGGWMRPERRRAALHFLPARV
                     HVVRQPLGVVGILSPWNYPVFLALSPLATAIAAGDRVMLKPSEHTPATSALIAEVVRE
                     VFPTEQATVVLGGPEVGAAFSALPFDHLFFTGGGTVARAVMRAASAHLVPVTLELGGK
                     SPVIVDRGYPLERAADAIASGKLFNAGQTCVAPDYALVPAESVGRFGELVARAAARLY
                     PTLGTNPDYTAIASDRHYDRLTGLVEDARARGATVMEVNPAGEDLRAGGGRKLAPTLL
                     TGVTDVMMVMQEEIFGPLLPILPYGDIQEAVDRINRGPRPLALYFFGGDTAARRTVLA
                     RTTSGGVCINDTQLQVLQDDLPFGGVGASGMGAYHGREGFLTFSHSKAVMRQPRLNLP
                     SLLRPPYGRLTERMIDYLLR"
     gene            complement(3869..4615)
                     /locus_tag="VFU45_05375"
     CDS             complement(3869..4615)
                     /locus_tag="VFU45_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5358524.1"
                     /translation="MDQKVVVITGASAGIGAALSETVARRGATPVLVARRADALEAVA
                     RKCSGRALVVAADVTRRAEVRGVVQETLERLGRIDVWVNNAGQGITRASTELTDDDVD
                     AMVRINVKSVLYGMQEVLPHFQARGEGHIINVSSMLGRVPMAPFRSAYVGAKHFMNAL
                     TATFRAEVQRTHPGIQVSLVSPGVVRTEFGVNAVHGGPDSRELPNSQSAEEVAAVIAD
                     VIESRRPDVYTRAGARKMVLDHYAASGEDP"
     gene            complement(4618..5109)
                     /locus_tag="VFU45_05380"
     CDS             complement(4618..5109)
                     /locus_tag="VFU45_05380"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HEU5358525.1"
                     /translation="MDLLQVSRVVATCCTGVASGMFIGYRMSVPPARPQLAPGAFIGL
                     MQAVRQRASATLPPLLFGALLGTVTWAAILARRGIHGTWPVALAAAGLAATIGVTYVV
                     HLPLEQELQTWRRDTPPTDLDARWAPWERWHRVCAWLLFLAFVLQVVVLAGGGSAWRL
                     RVG"
     gene            complement(5126..5686)
                     /locus_tag="VFU45_05385"
     CDS             complement(5126..5686)
                     /locus_tag="VFU45_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358526.1"
                     /translation="MTGIMALWLPILLSAVIVFIASSIIHMFTPWHKNDFAPLPDEGK
                     VAAALRPLNIPPGDYHLPRPTSMADMKSTAFAEKMKQGPVVMMTVMPSGPMSMGGSLV
                     GWFVFCAVVSLLAAYVAGLHIASGADYLTVFRTVSAVTFMGYVLGNWPNTIWYKRNLG
                     TMIRMSLDGLLFGFLTGGVFGWLWPR"
     gene            complement(5729..6100)
                     /locus_tag="VFU45_05390"
     CDS             complement(5729..6100)
                     /locus_tag="VFU45_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5358527.1"
                     /translation="MLANATVITFLATRDPERSRRFYAETLGLPLKADDGFALVFDAN
                     GIELRVQKVDRVEPHAYTALGWRVADISVKVAGLARGGVAFERYQGMTQDPSGVWQAP
                     SGARVAWFKDPDGNLLSLTQG"
     gene            complement(6275..6835)
                     /locus_tag="VFU45_05395"
     CDS             complement(6275..6835)
                     /locus_tag="VFU45_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEU5358528.1"
                     /translation="MRRFLIVYGTTDGHTAKVAAAVADRLRGSGAAVDVFNAGSTDPS
                     PGPYDGVIVAASVHAHRYQRSVERWVRRWAAVLAGHRTLFLSVCLATLQQDPAVQRDL
                     AQIVERFSTRTGWRPGRVQQVAGALPYTRYGWLKRMMMVRISRSTGGATDTSRDHEYT
                     DWAALGASVDEFRAACAPAEPSHAVT"
     gene            complement(6962..7369)
                     /locus_tag="VFU45_05400"
     CDS             complement(6962..7369)
                     /locus_tag="VFU45_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEU5358529.1"
                     /translation="MRPQLGSDIRLKLPLEAALTRTRDTLKQEGFGVLTEIDLEAAFK
                     EKLGKPFHPYRILGACNPPLAYSAVSADPSIGLLLPCNVTVEAPPDGGVIVRLTDPEA
                     LLGADGAGTSLAEVAKDARARLLRVHAALSRGA"
     gene            complement(7382..7921)
                     /locus_tag="VFU45_05405"
     CDS             complement(7382..7921)
                     /locus_tag="VFU45_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358530.1"
                     /translation="MPHRTGIYIVPTDHWYIERTVWVIAGAFLTGSTALALLSDPRWI
                     LLTTVTGLASIAVALTGFCVVGNVLIRLGFRPRLGDAARAGGGWYFMQTDRWYLERRI
                     YLVVGINITIASLLSLAWTPWWLAFIGFVGLAMFWFAATGFCIMANGLYWLGAEPRLA
                     PECARGRAPRPAKLAGFTR"
     gene            complement(8027..8353)
                     /locus_tag="VFU45_05410"
     CDS             complement(8027..8353)
                     /locus_tag="VFU45_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358531.1"
                     /translation="MRSLALALLLLLAGATTGAAQALPARAEMRAAAALQASGTAFAP
                     ASQQTPFAALLPASQYRSRRQGETLMIVGGAALLGGIIVGGSGGAVLALGGIGVGAYG
                     VYLYTR"
     gene            complement(8361..8954)
                     /locus_tag="VFU45_05415"
     CDS             complement(8361..8954)
                     /locus_tag="VFU45_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5358532.1"
                     /translation="MTPIHRADLVIDSARLRLRPLTPDDAPFILALVNDPDWIRYIGD
                     RGVRDLDDARRYVVDGPMASYARHGFGLYLVTRRSDAVPIGICGLIQRETLPAPDVGF
                     AFLPAFRRYGYAAEAVDALLRCARELANTEQLYAVVQPDNARSLHLLHQAGFRPAGTT
                     RLVPDGPELTLLSVALRDATPADRAERDHRRDRPSTT"
     gene            complement(8951..9424)
                     /locus_tag="VFU45_05420"
     CDS             complement(8951..9424)
                     /locus_tag="VFU45_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5358533.1"
                     /translation="MSDNTAVVAALYAALSRRDAAAMAACYAPDATFSDPVFVGLQGA
                     EVGAMWRMLCERATDLRVECRDIRVTGDAGAAHWEAWYSFSVTGRPVHNVIEATFEFR
                     DGLIVRHVDRFDLHRWARQALGPAGWLLGWTPPMQAAIRRKAAAGLARFRERPAR"
     gene            complement(9421..9888)
                     /locus_tag="VFU45_05425"
     CDS             complement(9421..9888)
                     /locus_tag="VFU45_05425"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEU5358534.1"
                     /translation="MKIWIDADAAPREVKDLVFRASRRLQVEVVLVANQRLQAPPGYP
                     LVTTTWVDGGADVADRHIAEHARKGDLVVTQDIPLAALLVPKGIAVLDPRGDEHTPET
                     IGERLSLRDFMEQARIAGAVTSGPPPWDARSTRAFAAALDRTLTRLRRPGQSP"
     gene            complement(9914..10669)
                     /locus_tag="VFU45_05430"
     CDS             complement(9914..10669)
                     /locus_tag="VFU45_05430"
                     /inference="COORDINATES: protein motif:HMM:NF033196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5358535.1"
                     /translation="MSRTAVISLALVIAAAPLAAQGPGGGKFPPDSLVNTRVIPHNTP
                     VREVLGTMRGFTMALGVRCQFCHQGEEGQPLSTYDFPSDRKETKLIARQMMRMVEEID
                     RRVDTLPGRAAGDPTVSCVTCHRGVSTPEPLPQLLADVAQANGADSAVRAYKALRQRY
                     YGRDAYDFGENSLPTAAFRLAQASKFAEAMALLSLNEENFPGASGTAVMRGNVQLLKG
                     DTAAAIAAYQEAVRRDSTDREARGRLQALQRRP"
     gene            complement(10745..>11024)
                     /locus_tag="VFU45_05435"
     CDS             complement(10745..>11024)
                     /locus_tag="VFU45_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358536.1"
                     /translation="AFGVWDAIRGAVSAAFAISCVGVIRQDRDFAWFAVIFAWCVAAI
                     QCVDAFLKIFHLTLSVPIGAVVYVAFAIKLSSNLKREVHTPPGSGGML"
BASE COUNT         1669 a         3791 c         4013 g         1551 t
ORIGIN      
        1 gctgatagct gatagctgat agctgatagc tgatagctac gtcgccgtca cgccctcggc
       61 gggcgtcgcg ctggcgtcgc tgatggccgc ctggacggcc gcgaccatcc gcgcggcgcg
      121 ctcccggtct ccgcgggaga tccacaaccg cagcagaccg tggttctcgc tcggcaccgg
      181 atcgagtgcc gcgagcgtcc gttcttccag ctcgatgaac ggcaggatcc atccgccgcg
      241 gaggcgcgca ctccggaggt ccgacgcggc cacgtcgagc gcctccagcg gcacggtgac
      301 cgtctcgttc ccggcctcga aaagctgccg ggtctcgacc ctcatggtgc cggtccattc
      361 gatggtgagc gtgtcgccgc cgaagtacac attgccgtgc acgcggaagc ggcgggtgat
      421 ccggccgagt ccctgcacgg cgcgctggcg ccggatacgc aggcggaagg acacgccacc
      481 gctcatgggg ccgccggatc cgcgcgcccg gccaggtcgg cctggtcggt gaacttgagc
      541 gactcggagt tgacgcagaa ccggagcccg gtgggcttcg gtccgtcctc gaacacgtgc
      601 cccaggtggc agtcgcagcg ggcgcagagg atctcggtgc gccgcatccc gaagctcacg
      661 tccgcgtggg tggtgacgtt ctccgccgcc accggctcga agaagctggg ccacccggtg
      721 ccggaatcga acttcccgcg ggaggaaaag agcgggagcc cgcagcagat gcaggtgtag
      781 atcccctctc tcttattgtc gagcagggtg ccgcagaagg ggcgctccgt ccccttgcca
      841 cgtgccacgg cgaactcctc cggggtgagc agtgcccgcc actcggcatc ggtcttctcg
      901 acgcgcgggg tctcgatcgg ccccacgagg cggccgttcc ggtcgatgag cttgaggcgc
      961 aaggatcacg ctccttcggt tcggtcaccc gtagtgcact gccagatgga tcaggttcac
     1021 cgctgccagc cacaccgggt ggtacagctt gtcgatgagc tgccacgcca ccacgatccc
     1081 gatgaggccg agtccgcggt gggtcatgac gaactcctgg tacttgtgcg ccccgtcgtc
     1141 gtgcaggaag agcggcggga ccccggcgcc gtcgagtggc gggaagggca gcaggttgaa
     1201 gaggcccagg agcaggttga gcgagaagaa tacgctcagc aggaacccgc cggcgcgcca
     1261 catcgtgtcg gggtcggtgg cctccacgat atggtcgaag ctcacgctct ccggtgcctg
     1321 gaagatgccg tgggccagcc ccaggtgcag gaagagcccc gccagcacca cgagcaggaa
     1381 gttggacgcc ggccccgcca gcgccatcag cgccgcgcgt cgcgggtggc ggagtgccca
     1441 ctcggggtcg aagggcgcgc tcgcaaagcc gatgggccac ccggaggcga tgacgccgag
     1501 gagcggcagc accaccatcc cgatcggctc gcgacggatg tgcgggatcg ggtcgagggt
     1561 cacctggccg ccgtggtacg cggtgggatc gcccccctga agcgcggccc aggcgtgcga
     1621 cgcctcgtgc agcgtcgtgg aaaagaggaa caccacgtaa tagatgaggc cggacgcgag
     1681 ttcgggcatc gcgaaagtta ccccgcgccc ggcccggtcg aaactccggg cccgtgagca
     1741 tccgtaggtt ccctgtcccc agcccaggaa ccttcatgcg tgctcccggt cagtggtacc
     1801 gcaccagcgc cgtcctcctc gcgtgcttgg ggttcctggt ggcgggaggg gcgctcgccc
     1861 aatccccgcc gcccgcatgt accgcgcccg agttccggca gttcgacttc tgggtcggcg
     1921 aatggtcggt gaccgacagc gccggcggca cgccgtacgg cacaagttcc gtcacgaatg
     1981 aagagggcgg atgcgtcatc cacgagcact ggcacagcgc ggggggcggc accgggcaga
     2041 gcttcaactt ctacaacgcg acccgtcggg actggacgca ggtctgggtg gcgagcagcg
     2101 ggggacaact ccacctggtc ggacacctga ccggcggggc gatggtgctc gagcaatcga
     2161 ccgtcgcccc gaatggcgcg ccggtggtcc agcggatcac ctggacgccc aagcccgacg
     2221 ggcgggtgcg acagatgtgg cagcagtccc gggatggcgg gaagacctgg acggtggcct
     2281 ttgacgggtg gtaccggcgg aaggggtgat cagggcgtct ctgagtagtg ggtggctggt
     2341 ggcctttcgt ggccgggcgc taccttatta gcagcgcgcg accggccgtg ccgcggttcc
     2401 cagtggccgg tcatccgtca ccagccactc ccaggcacct gcgatgctct ccaccccgga
     2461 tctctccgac ctcctcgcgc gccagcgtgc cgccttcatg gccgccgcgc cgccaagcgc
     2521 tgcgacgcgg cgcgccgatc tgcggcggct caaggcggcg atcgtgcggc gccgggcgga
     2581 gttctcggcc gcggccagca gcgacttcgg gcaccgctcg ccgcacgaaa cactgatcgc
     2641 cgacctgaac ccgacggtcc agtcgatccg ctacctggtc cgccatctcg gcggctggat
     2701 gcggccggag cggcggcgcg cggccctcca cttccttccg gcccgcgtcc acgtggtgcg
     2761 ccagcccctc ggcgtggtgg gcatcctgtc gccctggaat taccccgtgt tcctggcgct
     2821 ttcaccgctc gccaccgcca tcgcggcggg tgaccgcgtg atgctcaagc cttcggaaca
     2881 cactcccgcc acgtcggcgc tcatcgcgga ggtggtgcgc gaggtgttcc cgacggagca
     2941 ggcgaccgtc gtgctgggcg ggccggaggt cggggccgcg ttctccgcgc tcccgttcga
     3001 ccacctcttc ttcaccggcg ggggcaccgt cgcgcgcgca gtgatgcgcg cggcgagcgc
     3061 ccacctcgtg ccggtgacgc tcgagctcgg cgggaagtcc ccggtcatcg tggatcgcgg
     3121 ctatccgctc gaacgcgccg cggacgcgat cgcctcgggc aagctcttca atgccggcca
     3181 gacctgcgtg gcccccgact acgcgctggt gcccgcggaa tcggtcgggc ggttcggcga
     3241 gctcgtcgcc agggcggcag cgcggctcta cccgacgctc ggcacgaacc ccgactacac
     3301 ggccatcgcc agcgaccggc attacgaccg gctgacgggc ctggtcgagg acgcccgtgc
     3361 ccggggcgcc acggtgatgg aagtgaaccc cgccggcgag gatctgcgcg ccggcggtgg
     3421 gcggaagctg gcgccgaccc tgctcaccgg cgtcaccgac gtgatgatgg tcatgcagga
     3481 ggagatcttc gggcccctgc tcccgatcct gccgtatggc gacatccagg aggcggtcga
     3541 ccggatcaac cgggggccgc gcccgctggc gctctacttc ttcggcgggg acacggcggc
     3601 ccggcgcacg gtgctcgcgc gcacgacctc gggcggtgtc tgcatcaacg acacccagct
     3661 ccaggtgctg caggacgacc tgcccttcgg cggcgtgggg gcgagcggga tgggggcgta
     3721 ccacgggcgg gaggggttcc tgaccttcag ccattcgaag gccgtgatgc ggcagccgag
     3781 gctcaatctc ccttcgctgc tgcggccgcc gtacggtcgg ctgaccgagc ggatgatcga
     3841 ctacctgctg cggtaggggc gctccgcgtc agggatcctc gccgctcgca gcgtagtggt
     3901 cgagcaccat cttccgcgcc cccgcccggg tgtacacgtc gggccggcgc gactcgatca
     3961 cgtccgcgat caccgcggcc acctcttcgg cgctctggga gttcggcagc tcgcgcgagt
     4021 cgggaccgcc gtgcaccgcg ttcacgccga actcggtgcg gaccaccccg ggtgagacga
     4081 gcgacacctg gatccccgga tgggtccgct ggacctcggc ccggaaggtg gcggtcagcg
     4141 cgttcatgaa gtgcttggcg ccgacgtagg ccgagcggaa cggcgccatc gggacgcggc
     4201 ccagcatcga cgagacgttg atgatgtgcc cctcgccgcg ggcctggaag tgcggcagga
     4261 cctcctgcat cccgtagagc accgacttga cgttgatccg caccatcgcg tcgacgtcgt
     4321 cgtcggtcag ttctgtcgac gcgcgggtga tgccctggcc ggcgttgttg acccacacgt
     4381 cgatccggcc gaggcgttcg agcgtctctt gcacgacgcc gcgcacctcg gcacgccggg
     4441 tcacgtcggc ggcgaccacg agggcccgcc cactgcattt ccgcgccacc gcctcgaggg
     4501 cgtcggcgcg gcgggccacg agcaccggcg tggcgccgcg cctggcgaca gtctcagaga
     4561 gcgcggcccc gatcccggcg ctggcgccgg tgatgacgac gaccttctgg tccaagttca
     4621 gcccactcgc agacgccagg cgctcccgcc cccggcgagc acgacgactt gcagtacgaa
     4681 tgccaggaac aggagccagg cgcagacccg gtgccaccgc tcccacggcg cccagcgggc
     4741 gtcgaggtcg gtgggcgggg tgtcacggcg ccacgtctga agttcctgct cgagcggcag
     4801 gtggaccacg tacgtgacgc cgatcgtggc cgcgagcccc gccgcggcca gcgccaccgg
     4861 ccaggtgccg tggatcccgc gccgggccag gatcgcggcc caggtcacgg tgcccaggag
     4921 cgccccgaac aggagcggcg gcagcgtcgc agaagcgcgt tggcgcacgg cctgcatgag
     4981 cccgatgaag gcgccgggcg cgagctgtgg ccgggcgggc gggacactca tccggtagcc
     5041 gatgaacatc ccgctcgcga cgccggtgca gcacgtcgcg accacccggc tcacctgcag
     5101 caggtccacg acggcagccc gcccgttacc tgggccagag ccagccgaag acgcccccgg
     5161 tcaggaagcc gaagaggagt ccgtcgagcg acatacggat catcgtgccc aggttccgct
     5221 tgtaccagat ggtgttgggc cagttgccga gcacgtaccc catgaaggtc acggcgctca
     5281 ccgtgcggaa gaccgtcagg tagtcggcgc cggaggcgat gtgaagcccc gcgacgtacg
     5341 cggccaggag cgacacgacc gcgcagaaca cgaaccaccc caccaggctc ccgcccatcg
     5401 acatcgggcc gctcggcatc accgtcatca tcaccaccgg accctgcttc atcttctccg
     5461 cgaaggcggt gctcttcatg tccgccatgc tcgtgggacg gggcagatgg tagtcgcccg
     5521 gggggatgtt gaggggacgg agcgccgccg cgaccttccc ctcgtcaggc aggggcgcga
     5581 agtcgttctt gtgccacggc gtgaacatgt ggatgatcga gctggcgatg aacacgatca
     5641 ccgccgacag caggatgggg agccacaacg ccatgatacc ggtcacgggg tcctccgtgg
     5701 gaagggagac tgcctctgaa cgactcgatc agccctgggt cagcgagagc aggttgccat
     5761 cggggtcctt gaaccacgct acgcgggcgc cgctcggcgc ctgccacacg cccgacgggt
     5821 cctgcgtcat tccctggtac cgctcgaacg ccaccccgcc gcgggccagc cccgccacct
     5881 tgaccgagat gtccgcgacc cgccatccga gggcggtgta tgcgtgcggc tccacccggt
     5941 cgaccttctg cacgcggagc tcgatgccgt tcgcatcgaa cacgagggca aatccatcat
     6001 cggccttgag cggcaggccg agggtctctg cgtagaatcg ccgcgaccgt tccgggtccc
     6061 tggtcgcgag gaaggtgatg accgtggcgt tcgcgagcat cggtggctcc ggtcgagggg
     6121 tgtcgtcgtc cgaccccgac aatactacgg cgcggagggg cggtcggcca tatcgacgcg
     6181 tcccggggct ccggcccccg cgaacgcacg acaccccgcg accggcgggc cgcggggtgt
     6241 cgatcgacgc cgttcgtccc ggcgagcggg cggtctacgt caccgcatgc gacggttccg
     6301 cgggcgcgca cgcggcccgg aactcgtcca cgctggcgcc gagcgccgcc cagtcggtgt
     6361 attcgtggtc gcgcgaggtg tcggtggcgc cgccggtgga tcgcgagatg cgcaccatca
     6421 tcatccgctt gagccagccg tagcgggtgt acgggagtgc gccggccacc tgctgcactc
     6481 ggccggggcg ccagccggtg cgtgtcgaga accgctccac gatctgggcc aggtcgcgct
     6541 ggacggcggg atcctgctgc agggtggcca ggcacaccga gaggaagagg gtacggtggc
     6601 cggcgagcac cgccgcccag cggcggaccc agcgctccac gctgcgctgg taccggtgcg
     6661 cgtgcaccga cgccgcgacg atcacaccgt cgtatggccc tggggacgga tcggtcgacc
     6721 cggcgttgaa gacgtcgacc gcggcgccgc tgccgcggag ccggtcggcg acggcggccg
     6781 cgactttcgc ggtgtgcccg tcggtggtcc catagactat gaggaagcgt cgcatcgttc
     6841 cacccccggt gtcggccggg ctggatcgcc ccgaccggcc cggagcttac ggccggcgcg
     6901 atgcaacgac cgtgaatcgg cggtgaacct ccgcttacgt gcccggggcg cccccgcccg
     6961 gtcaggcgcc gcgggagagc gccgcgtgca cccggaggag gcgcgcccgc gcgtccttgg
     7021 ccacctccgc cagcgacgtg ccggccccgt ccgcgcccag gagcgcctcg ggatcggtga
     7081 gtcgcacgat cacgccgccg tccggcgggg cctcgacggt gacgttgcac ggcagcagga
     7141 gcccgatcga cgggtcggct gaaacggcgc tgtacgcgag cggcgggttg caagcgccca
     7201 ggatccggta gggatggaat ggcttgccga gcttttcctt gaacgccgcc tcgaggtcga
     7261 tctcggtgag gacaccgaag ccttcctgct tgagggtatc gcgggtgcgg gtgagcgcgg
     7321 cctcgagcgg gagcttgagc cggatgtcac ttccaagctg gggacgcatc gcagcctccg
     7381 gtcagcgggt gaagccggcg agcttggccg ggcgcggcgc ccgcccccgg gcgcactccg
     7441 gcgccaggcg cggttcggcg ccgagccagt agaggccgtt ggccatgatg cagaagccgg
     7501 tggccgcgaa ccagaacatc gcgaggccca cgaacccgat gaaggcgagc caccacggcg
     7561 tccaggcgag cgagagcagc gacgcgatcg tgatgttgat gcccaccacc aggtagatcc
     7621 gccgctcgag gtaccagcgg tcggtctgca tgaagtacca gccgccaccc gcacgggcgg
     7681 cgtcgcccag cctgggccgg aagccgagcc ggatgagcac gttgcccacg acgcagaagc
     7741 ccgtgagcgc caccgcgatc gaggcgagtc cggtgacggt ggtcagcagg atccagcggg
     7801 ggtcggacag gagcgcgagg gcggtgctgc cggtgaggaa cgcgcccgcg atgacccaga
     7861 cggtgcgctc gatgtaccaa tggtcggtgg ggacgatgta gatcccggtg cggtgcggca
     7921 tggcacgatc ctcggtcacg ggggcggaga agaaaggctt gcccccgtgc gctgaagtcg
     7981 cactgaaccg accgtgaagg aaccttcgtg agtggctggg actgcgtcag cgggtgtaga
     8041 ggtacacccc gtaggcgccg acgccgatcc cgcccagggc cagcaccgca ccgcccgagc
     8101 cgccgacgat gatcccgccg aggagtgctg cgccgccgac gatcatcagg gtctcccctt
     8161 ggcgccggct gcggtactgg ctggccggaa gcagggcagc aaagggggtc tgctgggaag
     8221 cgggcgcgaa cgccgtgccg ctcgcctgca gcgcggcggc ggcgcgcatc tccgcgcggg
     8281 ccggcagggc ctgggcggcg ccggtggtgg cgccggcaag gaggagcagg agggcgaggg
     8341 ctaggctgcg catgatggcc tcaggtcgtc gaaggacggt cgcgtcggtg gtcccgctcc
     8401 gcgcggtccg cgggtgtcgc gtcgcggagc gccaccgaaa ggagggtaag ctcggggccg
     8461 tcaggcacca agcgggtggt tccggcgggc cggaatccgg cctgatggag gaggtggagc
     8521 gaccgggcgt tgtcgggctg caccacggcg tagagctgct cggtgttggc gagttcccgt
     8581 gcgcatcgaa ggagcgcgtc gaccgcctcc gcggcgtagc cgtacctgcg gaacgccggc
     8641 aggaaggcga atccgacgtc gggggcgggg agggtctccc gctggatcag gccgcagatg
     8701 ccgatcggca cggcgtcgga ccggcgcgtc acgaggtaca ggccgaaccc gtggcgcgcg
     8761 taactcgcca tcggaccatc gacgacgtat cgccgtgcat cgtcgaggtc gcgcacgcct
     8821 cgatcgccga tgtagcggat ccagtcgggg tcgttgacga gcgcgaggat gaacggcgcg
     8881 tcgtcggggg tgagcgggcg gagccggagc cgcgcgctgt cgatcacgag gtcggcgcga
     8941 tggatcgggg tcaccgggcc ggccgttcgc ggaagcgggc cagcccggcg gcggccttgc
     9001 ggcggatggc ggcctgcatc ggcggcgtcc agccgaggag ccatccggca gggccgaggg
     9061 cctggcgggc ccagcggtgg aggtcgaacc ggtcgacatg cctgacgatc aggccatccc
     9121 ggaactcgaa ggtcgcttcg atgacattgt ggaccgggcg cccggtgacc gagaagctgt
     9181 accacgcctc ccagtgcgcg gcgccggcat cgccggtcac gcggatgtcg cggcactcga
     9241 ccctgaggtc ggtggcgcgt tcgcagagca tccgccacat ggcaccgacc tcggcaccct
     9301 gcaacccgac gaagaccggg tccgagaagg tcgcgtcagg cgcgtagcac gccgccatcg
     9361 ctgcggcgtc gcggcgggag agggccgcgt acagcgccgc gacgaccgcc gtgttgtcgc
     9421 tcatggtgac tggccgggcc gccggagccg ggtcagggtc cggtcgagcg ccgccgcgaa
     9481 tgcccgggtg ctccgcgcgt cccacggcgg cggtccgctg gtgacggcgc ccgcgatccg
     9541 cgcctgctcc atgaaatccc tcagcgagag ccgttcgccg atcgtctccg gcgtgtgctc
     9601 gtcgccgcgg gggtcgagga ccgcgatccc cttcgggacg aggagcgcgg ccagcgggat
     9661 gtcctgcgtc acaacgagat cccccttcct ggcgtgctcg gcgatatggc ggtcggcaac
     9721 gtcggcgccg ccgtcgaccc aggtcgtggt gacgagcgga taccccggcg gcgcctgcag
     9781 ccgctgattg gccaccagca cgacctcgac ctggagccgc cgggaggcgc ggaacaccag
     9841 gtccttcacc tcgcgggggg ccgcgtcggc gtcgatccag atcttcaggg cgtccccgct
     9901 cgcatcgcgc cggtcacggg cggcgctgga gcgcctggag ccggccccgc gcctcgcggt
     9961 cggtggagtc gcggcggacc gcctcctggt acgcggcgat ggcggcggcg gtgtcgccct
    10021 tgaggagctg gacgttgccg cgcatcactg cggtaccgga ggcgcccggg aagttctcct
    10081 cgttcagcga gagcagcgcc atcgcctcgg cgaacttcga cgcctgcgcc aggcggaacg
    10141 ccgccgtggg gagggagttc tcgccgaagt cgtaggcgtc gcggccgtag tagcgctggc
    10201 ggagcgcctt gtaggcgcgc accgccgaat cggcaccgtt cgcctgcgcc acgtcggcca
    10261 ggagctgcgg gagcggctcg ggggtgctca ctccccggtg acaggtgaca caggagaccg
    10321 tcgggtctcc cgcggcgcgg ccggggaggg tatccacgcg tcggtcgatc tcctccacca
    10381 tccgcatcat ctgccgggcg atgagcttgg tctctttccg gtcgctcggg aagtcgtagg
    10441 tcgagagcgg ctgaccttcc tcgccctggt ggcagaactg gcaccggacg ccgagcgcca
    10501 tcgtgaagcc gcgcatcgtg ccgaggacct cgcggaccgg cgtgttgtgg gggatgaccc
    10561 tggtgttgac gagcgagtcg ggcgggaact ttcccccgcc cggcccctgc gcggcgagcg
    10621 gcgccgcggc gatgacgaga gcaagggaga tgacggcggt acgggacacg gagacctcac
    10681 gtcgagaggt ggatcggtga agatccgaag aaactggtga tggtgccact gcccggacgc
    10741 gtggctacag catcccgccc gaaccgggag gggtgtgcac ttcgcgcttg aggttggacg
    10801 agagcttgat cgcgaacgcc acgtacacca ccgcgccgat cggcacggaa agtgtcaggt
    10861 gaaagatctt gagaaatgcg tccacgcact ggatcgcggc gacgcaccag gcgaagatca
    10921 cagcgaacca agcgaagtcg cgatcctgcc tgatgacccc gacgcaggag atcgcgaacg
    10981 ccgcgctcac cgccccccga atcgcatccc acacaccgaa tgcg
//
LOCUS       DASUAP010000209         7918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1164346, whole genome shotgun sequence.
ACCESSION   DASUAP010000209 DASUAP010000000
VERSION     DASUAP010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164346"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFU45_05440"
     CDS             complement(<1..441)
                     /locus_tag="VFU45_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358537.1"
                     /translation="MKLSRKWLEAFLRRPLELQDTVRRLALLGAPVDAVEPLHQELEG
                     VVVAVVDEVRPHPNADRLRLCTVDDGSGERRQVVCGAPNVTAGKRYPFARVGVTLPGG
                     VTLEKRKIRGELSEGMLCSARELGLGQDHDGILELDLTVAPGTPF"
     gene            complement(447..1577)
                     /gene="pheS"
                     /locus_tag="VFU45_05445"
     CDS             complement(447..1577)
                     /gene="pheS"
                     /locus_tag="VFU45_05445"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU5358538.1"
                     /translation="MTEHPVLAALAPRLALVPALDAAQLEAEETAVLGRKSGALSALL
                     KQLAELPEEERRRVGQAANHLKAQFEVAFAERRAVLAQASAEAARTSVDLSMPGRGRW
                     VGSLHPVTQVMDEILGIFRELGFVRVVGPEAETEWHNFTALNFPADHPAMDLHDTFYV
                     DTGDRRATEHDRRRGRSSGLFPAQERRSVPDRRRPDVGQVLLRTHTSPVQVRTLLQHR
                     PPIRAMMPGMVYRKDPFDQSHAPAFLQLEGLVVDEGVSFVDLKATLTHFARRFFSART
                     SVRFRPSYFPFTEPSAEMDVECQLCGGAGCGACKGTGWMEILGCGMVHPHVLRNCEVD
                     PERYTGFAFGMGPQRMAMQRYGITDIRLLYSGDMRFLSQFTG"
     gene            complement(1699..2058)
                     /gene="rplT"
                     /locus_tag="VFU45_05450"
     CDS             complement(1699..2058)
                     /gene="rplT"
                     /locus_tag="VFU45_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU5358539.1"
                     /translation="MPRVKNGVAHHARKKKIMKAAKGGFQSRSKLYKAAKENVERGMK
                     YAYRDRRRKKGDFRSLWIMRINAAARMHGLSYSRFMAGLKAAGVEVNRKVLADLAVHD
                     AEAFAKFAELATAKAGA"
     gene            complement(2071..2265)
                     /gene="rpmI"
                     /locus_tag="VFU45_05455"
     CDS             complement(2071..2265)
                     /gene="rpmI"
                     /locus_tag="VFU45_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU5358540.1"
                     /translation="MPKQKTKRAAMKRFKRTGSGKLKRYHANHSHILTKKARKRKNKL
                     KKGAYVSSADFPRVNRLLLA"
     gene            complement(2351..2911)
                     /gene="infC"
                     /locus_tag="VFU45_05460"
     CDS             complement(2351..2911)
                     /gene="infC"
                     /locus_tag="VFU45_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU5358541.1"
                     /translation="MSVFISSWRENTRLSTFEREKRTRINRQIRISPVRVITGDGEQL
                     GVLPIEEALAAAQERGLDLVEVAPTARPPVVKIMDYGKYKFEEAKAARAARKKQHVIQ
                     LKEVKFRPGIDDHDYEFKARHAREFLGDGNKVKVTMMYRGRQMAHLELGKAVLDRLAA
                     ELADIGKVEQDARLEGRNMSMVIGPK"
     gene            complement(3295..4239)
                     /locus_tag="VFU45_05465"
     CDS             complement(3295..4239)
                     /locus_tag="VFU45_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5358542.1"
                     /translation="MPLTSTFTVGSLTCHTLEAGLQHLDGGAMFGVVPKPLWTRRIPA
                     DERNRIPLAMRCLLIEHAAGLILVDTGLGNKEDAKFHDIYGVVNAGTKGATQLEDALA
                     ELGRRPEDIDWVVNTHLHFDHAGGNTLADGRVDAQSHRPSTTDMRPGDHASVRLAFPN
                     ATYVVQRGELDFARHTNERTRASYLPPNFDPVDRAGKWKLLDGPGEVFPGVSARLTPG
                     HVPFHQSIVIKDGGETAIFLGDVMPTAAHLPLPWIMGYDLEPLRTLESKRALLAQAGA
                     EGWMFIFEHDASVARGRAVSGAKGVELAEVVAAPVGGA"
     gene            complement(4380..5195)
                     /locus_tag="VFU45_05470"
     CDS             complement(4380..5195)
                     /locus_tag="VFU45_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU5358543.1"
                     /translation="MTGLAAVPEQTLVDRALERLAAGPCSAAELSRDVLGLPNAPTAV
                     AERIVVALLGADPRVRQLDDGRWSTIAVAGTSPLIEECGFAVLDVETTGTRPSDEDRV
                     TDVAVVVVHGERRELVFESLVNPGRPIPPWITEMTGISDATVAGAPSFEAIADPLLQA
                     LAGRVFTAHNARFDWGFVSAEFSRTRGLQLLGPRLCTVRLARALLPELPSRGLDPLMQ
                     HFGIENQARHRAAGDALATAELLGRLILLARERGARTYADLDAMAASSSRHKP"
     gene            complement(5192..6430)
                     /locus_tag="VFU45_05475"
     CDS             complement(5192..6430)
                     /locus_tag="VFU45_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEU5358544.1"
                     /translation="MPALHITSEIAPLEAVLVHTPGRELQAVTPGTREDYLYDDIIDF
                     ELAEREHDRFVAVLERFSKVYQIADLTAEILAKPEAREFLISKTMDVVPSDALAQQLQ
                     KLPAQELVKLLVQGAEEESGPIARALNEVGFALPPLPNLFFPRDIGMVIGDHAVVGSM
                     RYGVRWTEELLIKALFRFHESLRNEGFLYDGSEEKRSNYTLEGGDVHVLREDLLVVGF
                     SERSSPAALDQLCEVAFARTGITDVIVVVMPRVPTAIHLDMIFTHIDEELCLLFPPTF
                     VGPERFAVLHRKKGQASVREMPNFLAALRAVKLPLEPVFCGGSHRPNQEREQWGSGCN
                     TLAVRPGTILSYGRNEYTLTELEKSGFRIIESEEFLTTSRGGKGKKRERQVITFPGAE
                     LVRGGGGPRCMTLPLRRAGA"
     gene            6667..7047
                     /locus_tag="VFU45_05480"
     CDS             6667..7047
                     /locus_tag="VFU45_05480"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU5358545.1"
                     /translation="MEVAKEELVGDYRKLDVWRVADDVAVRVYTVTGGFPIAERFGLT
                     SQMRRAAVSVPSNIAEGCGRNGRGDLRRFIQVALGSANELDYQLSLSTRLGYVESPAA
                     LELHGEITRVRQMLGSMLRGLGRT"
     gene            complement(7191..7811)
                     /locus_tag="VFU45_05485"
     CDS             complement(7191..7811)
                     /locus_tag="VFU45_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU5358546.1"
                     /translation="MKKIADSTVRRLSLYLRFLEEFEGQGMATVSSEALAHRGGTTSA
                     QVRKDLSFFGSFGKRGLGYSVPELSTRLRDILGLGRRYRVVLIGAGKIGAALVQYRGF
                     RERGFDIISIFDADKAKIGHKWNGLTVEDVAHLEAALKARPADIAVLVTPPEAAQGIA
                     DRLVKTGIRAILNFAPVNLSVPDEVTVTNVNLALELEALSYALGNK"
BASE COUNT         1173 a         2723 c         2771 g         1251 t
ORIGIN      
        1 gaacggggtg ccgggcgcca ccgtgaggtc gagctcgagg atgccgtcgt ggtcctggcc
       61 gagcccgagc tcgcgggccg agcagagcat cccttcggag agctcgccgc ggatcttccg
      121 cttctccagg gtgacgcccc cggggagggt gacgccgacc cgcgcgaacg ggtagcgctt
      181 ccccgccgtg acgttggggg cgccgcagac cacctggcgc cgctcgcccg agccgtcgtc
      241 caccgtgcag aggcggagcc ggtcggcgtt gggatggggg cgcacctcgt ccaccaccgc
      301 cacgacgacg ccttcgagct cctggtgcag gggctcgacg gcgtcgaccg gcgcgccgag
      361 gagcgccagg cgccgcaccg tgtcctgcaa ctcgagaggc cggcggagga aggcctccag
      421 ccacttccgg gagagcttca tcggccttag cccgtgaact gggagaggaa gcgcatgtcg
      481 cccgagtaga gcagccggat gtcggtgatg ccgtagcgct gcatcgccat ccgctggggg
      541 cccatcccga aggcgaagcc ggtgtagcgc tcggggtcga cctcgcagtt gcgcagcacg
      601 tgcgggtgca ccattccgca gccgaggatc tccatccacc cggtgccctt gcaggcgccg
      661 caccccgccc cgccgcagag ctggcactcg acgtccatct ccgccgaggg ctcggtgaac
      721 gggaaatagg aggggcggaa gcgcacgctg gtgcgggcgg agaagaaccg tcgggcaaag
      781 tgggtgagcg tggccttgag gtcgacgaat gacacgccct cgtccaccac cagcccttcg
      841 agctggagga aggcgggcgc gtgcgactgg tcgaaggggt ccttgcggta gaccatcccc
      901 ggcatcatcg cgcggatcgg cgggcggtgc tggaggagcg tgcgcacctg caccggcgag
      961 gtgtgggtgc ggaggagcac ctggcccacg tcgggtcggc ggcggtcggg gacgctccgg
     1021 cgctcttgcg ccgggaagag ccccgagctc cggccgcggc ggcggtcgtg ctcggtggcg
     1081 cgccggtcgc cggtgtccac gtagaacgtg tcgtggaggt ccatcgccgg atggtcggcc
     1141 gggaagttga gggcggtgaa attgtgccac tcggtctcgg cctcggggcc gaccacgcgg
     1201 acgaagccga gctcccggaa gatgccgagg atctcgtcca tcacctgggt gaccggatgg
     1261 aggctgccga cccagcgccc gcggccgggc atcgagaggt cgacgctcgt gcgcgccgcc
     1321 tcggcgctgg cctgggccag caccgcgcgc cgctcggcga aggccacctc gaactgggcc
     1381 ttcaggtggt tggccgcctg gccgacgcgc cggcgctcct cctcgggcag ctcggccagc
     1441 tgcttgagga gcgcggagag cgcgccgctc ttgcggccca ggacggccgt ctcctccgcc
     1501 tcgagctggg cggcgtcgag ggcgggtacg agggccaggc gcggcgcgag ggccgcgagg
     1561 acgggatgtt cggtcatcgg gggtgtcacc gaaccgtgga agcgggatgc gcgcgtgggg
     1621 ccgaccgccg atggcgggcg accccacggg aaccttgcgc cgccgggccc ccgcgggggc
     1681 cggcggacgc gtgcggggtt acgcccctgc cttggccgtc gcgagctcgg cgaacttggc
     1741 gaacgcctcg gcgtcgtgca ccgccaggtc ggcgaggacc ttgcggttga cctcgacgcc
     1801 cgccgccttg agccccgcca tgaagcggct gtagctgagg ccgtgcatcc gggccgcggc
     1861 gttgatgcgc atgatccaga gggagcggaa atcgcccttc ttccggcgcc ggtcgcggta
     1921 ggcgtacttc atcccccgct cgacgttctc cttggccgcc ttgtacagct tgctgcgcga
     1981 ctggaagccg cccttggcgg ccttcatgat cttcttcttg cgggcgtggt gggctacgcc
     2041 gttcttgacg cggggcatgg tctgagctcc tcaggccagg agaagacggt tgacgcgcgg
     2101 aaaatcggcg gagctcacgt aggcaccctt cttgagcttg ttcttccgct tgcgcgcctt
     2161 cttcgtcagg atgtgcgaat ggttggcgtg gtagcgcttg agcttgccgc tcccggtgcg
     2221 cttgaagcgc ttcatcgccg cgcgcttcgt cttctgcttc ggcattgcgt ttctccgatt
     2281 gaccgggcac ctggggcacc cgaaccttga tggcgcttgc acacccgtac gggcgcagca
     2341 tgctgcaccc ctacttcggg ccgatcacca tcgacatgtt gcgcccttcg aggcgggcgt
     2401 cctgctccac cttgccgatg tcggcgagct ccgccgccag ccggtcgagc accgccttgc
     2461 cgagctccag gtgcgccatc tgccgccccc ggtacatcat cgtcaccttc accttgttgc
     2521 cgtcgcccag gaactcgcgg gcgtggcggg ccttgaactc gtagtcgtgg tcgtcgatcc
     2581 cgggccggaa cttcacttcc ttcagctgga tgacatgctg cttcttgcgg gccgcgcgcg
     2641 ccgccttcgc ctcttcgaac ttgtacttgc cgtagtccat gatcttgacg accggcggac
     2701 gggccgtcgg cgccacctcg accaggtcga ggccgcgttc ctgcgcggcg gcgagcgcct
     2761 cctcgatcgg caggacgccg agctgctcgc catcaccggt gatcacccgg accgggctga
     2821 tgcggatctg ccggttgatc cgggtccgct tttcgcgctc gaaggtgctc agtcgcgtgt
     2881 tctcccgcca agaggaaatg aaaacggaca cccgtgaccg gatgtccgac gaatgcgacc
     2941 cgcccccagg gcggcctcac ctcgctggac ccgatcgcat cgcgctgccg ccgcgagctt
     3001 ggggtgggag gccaccggcc tccgcttccg tactacgggg gacaaacgta gccgggacgg
     3061 gccggagggt caagctggac accccgccgg gccgaccgat cgcgggcacc aaaggggtgg
     3121 cctgccgcca ccccggaagg cgtgggacca cggagcacac gaagaccacg gagaggcacg
     3181 gagcaaagcc attcagtgcg gtcttgcacc gcagcgccgc ttgcgagacc acatcacatg
     3241 cagttctccg tgattctccg tgccgctccg tgccctccgt ggtcccacct tttcctacgc
     3301 gccaccgacc ggcgccgcca ccacctccgc caactccacc cccttggccc ccgacaccgc
     3361 ccgcccccgc gccacgctcg catcgtgttc gaagatgaac atccacccct ccgcccccgc
     3421 ctgggccagg agcgcgcgct tcgactccag cgtccggagc ggctccaggt cgtaccccat
     3481 gatccagggg agcggcaggt gggcggccgt gggcatcacg tcgccgagga agatggcggt
     3541 ctccccgcca tccttgatca cgatcgattg gtggaagggc acgtgccctg gcgtgagccg
     3601 cgcggagacg ccgggaaaga cctcccccgg accgtcgagc agcttccact tccccgcccg
     3661 gtcgaccggg tcgaagttgg gcgggaggta gctggcgcgc gtgcgctcgt tggtgtgccg
     3721 ggcgaaatcg agctccccgc gctggacgac gtaggtcgcg ttcgggaaag cgaggcggac
     3781 ggacgcatgg tcgcctgggc gcatgtcagt ggtggacggg cgatgcgact gtgcgtccac
     3841 ccgcccatcc gccaacgtat tcccccccgc gtggtcgaaa tgcaggtggg tattcaccac
     3901 ccagtcgatg tcctccggcc ggcggccgag ctcggccagc gcgtcctcca gctgggtggc
     3961 gcccttcgtc cccgcgttca cgacgccgta gatgtcgtgg aacttggcgt cttccttgtt
     4021 gccgagcccg gtgtcgacca ggatgagccc ggccgcgtgc tcgatcagga ggcagcgcat
     4081 cgcgagcggg atccggttcc gctcgtcggc cggaatgcgc cgggtccaga gcggcttcgg
     4141 caccacgccg aacatcgccc cgccgtcgag gtgctgcagc cccgcctcga gggtgtggca
     4201 ggtgagggag ccgaccgtga aggtggaggt gaggggcatg gaccgtgagc ctcagaagat
     4261 ctctcggagg agtgggggtc gcctgcggcg acccaagtca ggcggccact gtcgggtcgc
     4321 cgcaggcgac ccccacgccc aaagtgcgaa gaccacactc gggcggccgc aggccacccc
     4381 tacggcttat gccggctgga cgatgctgcc atcgcatcaa gatccgcgta ggtccgcgcc
     4441 ccgcgctccc gcgccagcag gatgagccgc ccgaggagct ctgcggtcgc cagcgcgtcc
     4501 cccgcagccc ggtggcgcgc ctggttctcg atcccgaagt gctgcatcaa cggatccagc
     4561 ccccggctcg gcagctccgg caggagcgcc cgcgccagcc gcaccgtgca gagccggggc
     4621 ccgaggagct gcagtccccg cgtccggctg aactccgccg acacgaagcc ccagtcgaag
     4681 cgcgcgttgt gggcggtgaa cacccggccc gcgagcgcct gcagcagcgg atcggcgatc
     4741 gcctcgaagc tcggcgcccc ggccaccgtc gcgtcggaga tcccggtcat ctcggtgatc
     4801 cacggcggga tgggccggcc ggggttcacc aacgactcga acaccagctc ccgccgctct
     4861 ccgtgcacca ccaccaccgc cacgtcggtg acccggtcct catcgctcgg ccgggtcccg
     4921 gtggtctcga cgtcgagcac ggcgaagccg cactcctcga tcagcgggct cgtccccgcc
     4981 accgcgatgg tgctccagcg cccgtcgtcg agctgccgga cccgggggtc ggcgccgagg
     5041 agcgccacca cgatccgctc cgcgaccgcc gtgggcgcgt tggggaggcc gagcacgtcg
     5101 cgggagagct cggccgcgga gcagggcccg gcggcgagcc gctcgagggc gcggtccacc
     5161 agggtctgct ccgggaccgc ggcgagccca gtcacgcgcc tgcgcggcgg agggggagcg
     5221 tcatgcagcg ggggccgccg ccgccgcgca ccagttcggc gccgggaaag gtgatcacct
     5281 ggcgctcgcg cttcttgccc ttccccccgc gcgaggtggt caggaactcc tcgctctcga
     5341 tgatacggaa ccccgacttc tcgagctccg tgagcgtgta ctcgttccgg ccatagctca
     5401 ggatcgtgcc ggggcgcacg gccagcgtgt tgcagccgct cccccactgc tcccgctcct
     5461 ggttgggccg gtgggagccg ccgcagaaga ccggctcgag cgggagcttc accgcgcgga
     5521 gggccgccag gaagttgggc atctcgcgca cgctcgcctg ccccttcttc cggtgcagca
     5581 cggcgaagcg ctccggcccc acgaaggtcg gcgggaagag gaggcagagc tcctcgtcga
     5641 tgtgggtgaa gatcatgtcg aggtggatgg ccgtcggcac ccgcggcatc accacgacga
     5701 tgacgtcggt gatcccggtg cgggcgaacg ccacctcgca gagctggtcg agtgccgccg
     5761 ggctcgagcg ctcgctgaac ccgacgacga gcaggtcctc gcgcagcacg tgcacgtcgc
     5821 cgccctcgag ggtgtagttg ctccgcttct cctcggagcc gtcgtagagg aatccctcgt
     5881 tcctgagcga ctcgtggaag cggaagagtg ccttgatgag gagctcctcg gtccagcgga
     5941 cgccgtagcg catcgagccg accaccgcgt ggtcgccgat gaccatcccg atgtcgcggg
     6001 ggaagaagag attggggagc ggcgggagcg cgaaccccac ctcgttgagc gcccgggcga
     6061 tcggcccgct ctcctcttcc gccccctgca ccaggagctt gaccagctcc tgcgccggca
     6121 gcttctgcag ctgctgcgcc agcgcgtcgg aggggaccac gtccatcgtc ttcgagatca
     6181 ggaactcccg cgcctccggc ttggccagga tctcggcggt caggtcggcg atctggtaca
     6241 ccttggagaa tcgctccagc accgcgacga accggtcgtg ctcccgctcg gcgagctcga
     6301 agtcgatgat gtcgtcgtag aggtagtcct cccgggtgcc cggcgtgacc gcctgcagct
     6361 cgcggccggg ggtgtggacc aggacggctt cgagcggggc gatctcggag gtgatatgga
     6421 gggcgggcat gggggaaagc taattggaga tttcgatatc cgttatccgc tatccgttat
     6481 ccgctatccg ctatccgttg tccgctgggc agttgaccat ccggatccac cgaatgacgt
     6541 ccgtttcccg ctatccgcta tccgctaccg tcatccgata tccggagacc ggggcggcca
     6601 gtaggtgcgt tcgttggcca ccagtcgatc aggaatgatg acgggatcgg cgacccgccg
     6661 ccgaagttgg aggttgccaa ggaggaactg gtgggcgatt accggaaact tgatgtgtgg
     6721 cgggtcgcgg atgacgtggc ggtccgcgtc tacactgtca ctggcggctt cccgatcgcc
     6781 gagcgatttg gattgacgtc acagatgcgg cgggcagcgg tgtcggtccc gtcgaacatc
     6841 gccgaagggt gtgggcggaa cggtcggggc gatctgcgtc ggttcatcca agtggcgctc
     6901 ggctccgcca atgagcttga ctaccagctg agcctctcca cgcgactggg atacgtggaa
     6961 tcgcccgccg cgctggaact gcacggcgag atcacgcggg tccggcagat gcttgggagc
     7021 atgctgcgtg ggctcggaag gacatgaggg ggattccgct atccgctatc cgctatccgc
     7081 tatccgctat ccgctatccg ctatccggga acggacgagc agtgcgcccg atggcgcaag
     7141 tgggacttca agtggaacgg atcatcggat agcggatagc ggatagcgat ctacttgttc
     7201 cccagcgcat acgacagcgc ctcgagctcg agcgcgaggt tcacgttcgt caccgtcacc
     7261 tcatccggca ccgacaggtt gaccggcgcg aagttgagga tggcgcggat ccccgtcttg
     7321 accaggcggt ccgcgatccc ctgcgcggct tccggcggcg tcaccagcac ggcgatgtcg
     7381 gcggggcggg ccttgagcgc cgcctcgagg tgggccacgt cctcgaccgt gaggccgttc
     7441 cacttgtggc cgatcttggc cttgtcggcg tcgaagatcg agatgatgtc gaagccgcgt
     7501 tcgcggaagc cgcggtactg gaccagggcg gcgccgatct tccctgcccc gatgaggacc
     7561 acgcgatagc ggcggccgag ccccaggatg tcgcggaggc gggtggagag ctcggggacc
     7621 gagtagccga ggccgcgctt gccaaaggaa ccgaagaagg agaggtcctt ccggacctgg
     7681 gcggaggtgg tgccgccgcg gtgcgccagc gcctcggagg agaccgtggc catcccctgg
     7741 ccttcgaact cttccaggaa gcggagatat agggagagac ggcgcaccgt cgaatcagcg
     7801 atcttcttca tcatcggcat tgtgaaagat ttcacaatat cgccgcgatg gacggcccgc
     7861 gtcaatgacc tcaccccccg gccgcgtctc gccgagaaac ctcacccccc cggccgcg
//
LOCUS       DASUAP010000210         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_700787, whole genome shotgun sequence.
ACCESSION   DASUAP010000210 DASUAP010000000
VERSION     DASUAP010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700787"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VFU45_05490"
     CDS             <1..873
                     /locus_tag="VFU45_05490"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU5358547.1"
                     /translation="LEVTEVDLAPSVPARVTQVRVEEGDHVRSGDTVAVLTQAALGDQ
                     VTEARARVASVEARLRELTTGSRAAEIHRAEADSAAAAAAADRAAKELARIKALVPSG
                     DTTPQQLDDATAAASEAASRQAAAGATLRLVREGPRSEDVAAQQAELARARAALAALE
                     STRRDLVLTSPVDGAVISRNAEPGEVLATGVPAVTLGEVKRPYTRIYLGPDVLPDIRV
                     GEPATGVLDGFPDRRYRGRVVAIATQAEFTPRVALTESERRDLLFGVKVAFDDTTGVL
                     TAGLPITVTIGAAR"
     gene            870..1613
                     /locus_tag="VFU45_05495"
     CDS             870..1613
                     /locus_tag="VFU45_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358548.1"
                     /translation="MTELAIHTRGLRKVFGDVVAVEGLDLDIPRGQILGLLGANGSGK
                     TTTIRMLCGLLEPTAGDATVVGFDVRRDAERIRRRIGYMSQKFGLYDDLTVVENLRFY
                     SSLYGLRGALQAERIEQLLGELSVGGRVRQLVGQLSGGWRQRVALGCAIAHEPDLLLL
                     DEPTAGVDPAARKLFWERIYRLAESGVTILVTTHYMDEAERCNRLAVISRGVLLATGS
                     PDEIRAHFGAATLEDVFIELQHRSGELVG"
     gene            1610..2734
                     /locus_tag="VFU45_05500"
     CDS             1610..2734
                     /locus_tag="VFU45_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5358549.1"
                     /translation="MRWLRALHHSDVWPMLWKEFLQLRRDRLTIAMMVGLPAIQLLLF
                     GYAIRTEVRHLPTVVLDESRTPESRALVARMINTGNFDLAATVPDRDAIRQWIENGRA
                     AAAIVIPPAYARDLKRGRGAEAQVIVDAADPMSSAAALSGAQLAGEAEALAWTRMAGP
                     PPVQVRVRPWYNPGQRSAVYIVPGIIGVLLSMTLVLITSVSIVREREKGTLEQLIVTP
                     IGKLSIMIGKLAPFVLVGYVQITVILLLGILLFHVPVAGDVALLYLLALPFIVANLGL
                     GLLLSTLVRTQSQAMQLGFLFLLPNILLSGFMFPRAAMPALAQWIGAALPLTYFLEVL
                     RGILVKGVAAGSLIPEAAALTLFSIGTIAVSVRRFAKTIE"
BASE COUNT          384 a         1089 c         1016 g          436 t
ORIGIN      
        1 ctggaagtga ccgaggtgga cctcgcgccg tcggtcccgg cgcgcgtcac ccaggtccgg
       61 gtggaggaag gagaccacgt tcgttccggt gacacggtgg ccgtgcttac ccaggcggcc
      121 ctgggcgacc aggtgacgga agcgcgcgcg cgcgtggcct cggtggaagc gcggctccgg
      181 gagctcacca ccggctcgcg cgccgccgag atccaccgcg ccgaggccga ctcggcggcg
      241 gccgcggctg ccgcggaccg cgccgccaag gagttggcgc ggatcaaggc gctggtgccg
      301 agcggcgaca cgacgccgca gcagctcgac gacgccaccg ccgccgccag cgaggccgcc
      361 agccgtcagg ccgccgcggg ggcgaccctg cgcctggtcc gcgaagggcc gcgctcggag
      421 gacgtcgcgg cccagcaggc ggagctcgcg cgggcccgcg cggcgctcgc ggcgctcgag
      481 tcgacccgcc gggacctggt gctgacgagc ccggtggacg gagcggtcat ttcccgcaac
      541 gccgagcccg gggaggtgct cgccaccggt gtccctgcgg tgacgctggg cgaggtgaag
      601 cgcccgtata cccggatcta cctcggcccc gacgtcctgc cggacatccg cgtgggcgag
      661 cccgccaccg gggtgctcga cggcttcccg gaccggcggt atcgcggccg cgtggtcgcg
      721 atcgcgacgc aggcggagtt cacgccgcgg gtggcgctca ccgagagcga gcggcgcgac
      781 ctcctcttcg gcgtcaaggt cgccttcgat gacaccaccg gcgtgctcac ggcggggctt
      841 ccgatcacgg tgaccatcgg ggccgctcgg tgaccgagct cgccatccac acccgcgggc
      901 tccgcaaggt cttcggggac gtcgtggcgg tcgaggggct cgacctcgac atcccccgtg
      961 gccagatcct cggactcctg ggcgccaacg gcagcggcaa gaccaccacg atccggatgc
     1021 tctgcggcct gctcgagccg accgcggggg acgcgaccgt ggtcggattc gacgtccgtc
     1081 gcgatgccga gaggatccgc cgacggatcg ggtacatgtc ccagaagttc gggctctacg
     1141 acgacctgac ggtggtggag aacctccgct tctactcgtc gctgtacggc ctgcgcggcg
     1201 cgctccaggc cgaacggatc gagcagctcc tcggggagct gagcgtcggc ggccgggtgc
     1261 gccagctggt gggccagctg agcggcggct ggcgccagcg ggtggccctg gggtgcgcca
     1321 tcgcccatga gcccgacctc ctgctccttg acgaaccgac cgccggcgtc gacccggcgg
     1381 cgcgcaagct cttctgggaa cggatctacc ggctggcgga gtcgggggtc acgatcctcg
     1441 tcaccaccca ctacatggac gaggcggagc ggtgcaatcg gctggccgtg atcagccgcg
     1501 gggtgctgct cgccaccggc tcgccggatg agatccgggc gcacttcggc gccgcgacgc
     1561 tcgaggacgt gttcatcgag ctccagcacc ggagcggaga gctcgtcgga tgaggtggct
     1621 ccgtgcgctg caccactcgg atgtctggcc gatgctctgg aaggagttcc ttcagcttcg
     1681 gcgcgaccgc ctcaccatcg cgatgatggt gggcctcccg gccatccagc tgctgctctt
     1741 cggctacgcg atccggaccg aggtgcggca cctgccgacc gtcgtgctcg atgaatcccg
     1801 gacccccgag agccgggcgc tcgtggcccg gatgatcaac accggcaact tcgacctcgc
     1861 cgccacggtg cctgaccggg acgccatccg gcaatggatc gagaacggtc gcgcggcggc
     1921 ggccatcgtg atcccgcctg cctacgcccg cgatctcaag cggggccgcg gggccgaggc
     1981 gcaggtcatc gtcgatgccg ccgacccgat gtcctccgcc gccgcgctct ccggtgccca
     2041 gctcgccggc gaggccgagg cgctggcgtg gacccggatg gcgggcccgc cgccggtcca
     2101 ggtgcgggtc cgcccgtggt acaatccggg ccagcggagc gcggtgtaca tcgtccccgg
     2161 catcatcggc gtgctcctgt cgatgacgct ggtgctcatc accagcgtgt cgatcgtgcg
     2221 ggagcgggag aaagggacgc tggagcagct catcgtgacg ccgatcggca agctcagcat
     2281 catgatcggc aagctggccc cgttcgtact cgtgggctac gtgcagatca cggtgatcct
     2341 cctcctcggc atcctgctct tccacgtccc cgtcgcgggc gacgtggcgc tcctctacct
     2401 cctggccctg cccttcatcg tggccaacct cgggctcggc ctcctcctct ccacgctcgt
     2461 ccgcacccag tcgcaggcga tgcagctcgg gttcctcttc ctgctcccca acatcctgct
     2521 gtcggggttc atgttccccc gcgccgcgat gccggcgctg gcccagtgga tcggcgcggc
     2581 gctgccgctc acgtacttcc ttgaggtcct ccgcggcatc ctggtcaagg gggtcgccgc
     2641 gggctcgctc attcccgaag cggcggcgct cacgctcttc tcgatcggga ccatcgcggt
     2701 gagcgtccgc cggttcgcca agacgatcga gtgacggcgc gttcgccggc cccaccccgc
     2761 acgctcccga tgacgctccc ccgcccccgc cgtgccatcc tgtacctacg gcgaccccgg
     2821 cgcggtcaga gcaatccacg gcccctttga tccgttcccc tacgctcccg gatccgcgcc
     2881 cggggctccg ccgctcccta acgtcccccc tctcccgcag ggaga
//
LOCUS       DASUAP010000211         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_387456, whole genome shotgun sequence.
ACCESSION   DASUAP010000211 DASUAP010000000
VERSION     DASUAP010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387456"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFU45_05505"
     CDS             complement(<1..598)
                     /locus_tag="VFU45_05505"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358550.1"
                     /translation="MSTRWEEFRSRAQHGGMVPLTREVVLDGDTAVAAFQKVHRGPWG
                     FLLESLEGGEKWARYSFFGTEPREVYRYRGRAVERWTRDRGWRPSAIDVAPLDHLAET
                     LRRYPAVDVPGLPRFTGGAVGFIGYDVVRTIEDLPDAPVDDRDLPDAVLMLADTLVIV
                     DNVFHRAIVVASVATPAGATDDVLRPLFDQAEARLAEWL"
     gene            complement(595..1077)
                     /locus_tag="VFU45_05510"
     CDS             complement(595..1077)
                     /locus_tag="VFU45_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358551.1"
                     /translation="MTTETIRADQALILEDDGADPADTTLTIVPGRPRTIVIRHPDPD
                     NTVFATLEIPAGGLGDDDAPVALTLATRPGIYGLEVRTDAPFGPGVVLTFKYPVHFAP
                     PAAAIARYGSRAAFERRLVIARRNADGTLALLASGRPAGDNLSALLNTPGTYVVAAPR
                     "
     gene            1186..>2216
                     /locus_tag="VFU45_05515"
     CDS             1186..>2216
                     /locus_tag="VFU45_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358552.1"
                     /translation="MIHRVRWWWSVAPLVLGGALQAQGWDAPGARMLAERATLRRERG
                     GAADPSWRARAHGFVFFLAQVGDGLAGPPRLVRADELDVEVYRNAPGPSKQVIRGWRS
                     RSWLPTDIAYHRDHLGIVTDGFGDRIRIGDGDEVRDVPHPLSPAGLDLYSFALTDSVR
                     VTSGATTLVLDRLDVRPRDLQAGGVVGSFYVDRASAEVVRFRFGFTRAAYVDPELEDI
                     TVSLESAFVDQRAWLPYRQEVELRRQTRWLDFPARGIIRGRWEIGEYDLAPPPIPLPV
                     AAGPPIGGLLTPGDSARPWGATLDERLRAQALPLPGDALADVRRALANEAPQAALDRL
                     ESARLHAPRV"
BASE COUNT          274 a          824 c          826 g          292 t
ORIGIN      
        1 ccagccactc cgccaggcgc gcctcggcct ggtcgaagag cggccgcagc acgtcgtcgg
       61 tcgccccggc gggcgtcgcc acgctggcca cgacgatggc gcggtggaag acgttgtcga
      121 cgatgaccag cgtgtcggcc agcatcagga ccgcatcggg gaggtcacgg tcgtcgaccg
      181 gcgcgtcggg gaggtcctcg atcgtgcgca ccacgtcata cccgatgaac cccactgcgc
      241 cgccggtgaa ccgcggcaac cccggcacgt cgaccgccgg gtaacgccgg agggtctccg
      301 ccaggtgatc gagcggcgcc acgtcgatgg cggagggccg ccacccccgg tcgcgggtcc
      361 agcgctcgac cgcccggccc cggtagcggt acacctcgcg cggttcggtg ccgaagaagg
      421 agtagcgggc ccacttctcg ccgccctcga gcgactcgag caggaacccc cacggcccgc
      481 ggtggacctt ctggaacgcg gccacggcgg tgtcgccgtc gaggaccacc tcgcgggtga
      541 gcggcaccat tccgccgtgc tgggcgcgcg agcggaactc ctcccagcgt gtgctcatcg
      601 cggcgccgcc acgacgtacg tgcccggggt gttgaggagc gcgctcaggt tgtcgcccgc
      661 gggccgcccg gaggcgagga gcgccagcgt cccgtcggcg ttgcgccgag cgatgaccag
      721 gcgccgctcg aacgccgcgc gggagccgta tcgggcgatg gccgcggccg gcggggcgaa
      781 gtggaccggg tacttgaacg tgagcacgac accggggccg aagggcgcat cggtgcgcac
      841 ctcgaggccg tagatgccgg ggcgggtggc cagcgtcagc gccacgggag cgtcgtcatc
      901 gcccaggccg ccggccggga tctccagcgt ggcgaagacg gtgttgtcgg ggtcggggtg
      961 ccggatcacg atggtgcggg gtcggccggg gacgatggtc agcgtggtgt ccgcggggtc
     1021 ggcgccgtcg tcctcgagga tgagggcctg gtcggcgcgg atggtctctg tcgtcaccgg
     1081 gacggggatc gcgttcggat ccggcgccac ccggggccgg ccatggcagg cggccgcggc
     1141 caccgctacg cttgcaactg cggcccgtcg gagtaacgtc acgtgatgat tcaccgggtc
     1201 cgctggtggt ggtcagtggc gccgctggtc ctgggcggtg cgctccaggc gcagggatgg
     1261 gacgccccgg gcgccaggat gctggcggag cgggccaccc tgcgccggga gcgtggcggc
     1321 gccgcggacc cctcgtggcg cgcccgcgcg cacggcttcg tgttcttcct ggcccaggtc
     1381 ggcgacgggc tcgcgggtcc gccccgcctg gtgcgcgccg acgaactcga cgtcgaagtg
     1441 taccggaacg cccccggccc cagcaagcag gtcatccgtg gctggcgcag ccgcagctgg
     1501 ctccccaccg acatcgccta tcaccgcgac cacctcggca tcgtcaccga cggattcggc
     1561 gaccgcatcc gcatcggcga cggcgacgag gtgcgcgacg taccccaccc gctctccccg
     1621 gccggcctcg acctctactc cttcgcgctg accgactcgg tccgggtcac ctccggcgcc
     1681 acgacgctcg tgctcgatcg gctcgacgtc cgcccgcgtg acctccaggc gggcggggtg
     1741 gtgggctcgt tctacgtcga ccgggcgagc gcggaggtgg tgcggttccg gttcggcttc
     1801 acccgtgcgg cgtacgtcga ccccgagctc gaggacatca ccgtctcgct cgagagcgcc
     1861 ttcgtggacc agcgggcctg gctcccgtac cggcaggagg tcgagcttcg ccgccagacc
     1921 cgatggctcg atttcccggc gcgcggcatc atccgcggcc gctgggagat cggcgagtac
     1981 gacctcgcgc cgccgccgat ccccctgccg gtggccgccg ggcccccgat cggcggcctc
     2041 ctgacacccg gcgactcggc ccgcccctgg ggcgccacgc tcgatgagcg gctgcgggcc
     2101 caggcgctcc cgctccccgg cgacgccctg gccgacgtgc ggcgcgccct ggccaacgag
     2161 gcgccgcaag cggccctcga ccggctcgag tcggcccgcc tccacgcgcc acgggt
//
LOCUS       DASUAP010000212         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_605508, whole genome shotgun sequence.
ACCESSION   DASUAP010000212 DASUAP010000000
VERSION     DASUAP010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605508"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VFU45_05520"
     CDS             complement(<1..1371)
                     /locus_tag="VFU45_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEU5358553.1"
                     /translation="MTRRDILQAGAALSAAALTLPVRPADLREPASAGAPPTARDRLL
                     LDLGWRFHLGNADDPARDFGYGTGDLFAKAGEFFGPSAADFDDSAWSQVDLPHDWAVD
                     LPFINDPDEVGHGSKPLGRKYPDTSIGWYRRTFDIPAADSGRRISLEFDGVFRDCTAA
                     LNGHYLGRNLSGYAPFRYDVSDLVTYGGTNILVLRVDATEHEGWFYEGAGIYRHVWLV
                     KTDSLHVPQWGTVVTSEMGPGGAALTVRTEVANDAGSAASVRVRSTVLDPDGRRVAAA
                     TGSPMRVEAGATREATQTLHVAAPKPWSLETPVLYRMVTETLAGARVTDRTETSFGIR
                     TMHFDPDHGFFLNGTRVELKGTCNHQDHAGVGSALPDRLQEYRIAKLKEMGSNAYRTS
                     HNPPTPELLDACDRLGMLVLDETRMFSPEPEGLGQLERMIRRDRNHPCVFAWSIANEE
                     WAVQGND"
     gene            complement(1374..2702)
                     /locus_tag="VFU45_05525"
     CDS             complement(1374..2702)
                     /locus_tag="VFU45_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5358554.1"
                     /translation="MNLIAVATILSGATNLALAFSFFYAWQTILRRRYVALLAVAATF
                     GVAELLLQLPQHLAQGRGAAAAYVAAAAAGSFGTVAWVGGCYDFVRRRIPWLALGAAF
                     TALVAWSIIGPQVTGGFFARESPDSLIKGALGLWIAVTFWRGPAIGVRGVLTALFALQ
                     GLHQLDYPFLADKTWGVIAGLAVSDFLGITISVFLLIVVIEEARRETLAANESLRRAE
                     AMAEMGELVGGVAHEVRNPLMAISSGLQTLAVAEADAAARRADIFDDLKSAVTRLTTL
                     TRDLLEYGKPTDPDLQPGDAAAVIRSAAVACAPLATAAGVRIRIDAGDPVPTAVMDRS
                     RLIQVFVNLITNAVQHSPAGGEVRVTIRQQGGGGLVECEVADSGPGFPDDLLPHLFHP
                     FAGRRPGGTGLGLAIARRLVEQQRGTVAAQNRPDGGAFVRVTLSATRMHP"
     gene            2842..>3467
                     /locus_tag="VFU45_05530"
     CDS             2842..>3467
                     /locus_tag="VFU45_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358555.1"
                     /translation="MRLAASASLFLLLVGPTSTPRTGTPSIPDADRSRIAEAFRVADG
                     VGDRIWPGWHAVPFAILLITDSTEFLIHHPHPTSDFTPAGTDPALGPIWVRPRRLPVT
                     LLATAPFVGGIPTIAVGRAEAVGQSSVEWVLTLLHEHFHQLQYGQPGYYAAVAALGLA
                     HGDSTGMWMLNYPFPYDSVPVVRAIDSLAAALVTVLDAARPGADAPAP"
BASE COUNT          494 a         1231 c         1240 g          502 t
ORIGIN      
        1 gtcgttcccc tggaccgccc actcctcgtt cgcgatggac caggcgaaga cgcaggggtg
       61 gttccggtcg cggcggatca tccgttcgag ctggcccagg ccctcgggct cgggcgagaa
      121 catccgggtc tcgtcgagca cgagcatccc cagccggtcg caggcgtcga gaagctcggg
      181 ggtcggcggg ttgtgtgagg tgcggtacgc gttcgacccc atctccttga gcttcgcgat
      241 ccgatactcc tgcagccggt cgggcagggc cgagccgacg ccggcgtggt cctggtgatt
      301 gcaggtcccc ttcaactcga cccgcgtgcc gttgaggaag aagccgtggt ccgggtcgaa
      361 gtgcatcgtg cggatgccga aggaggtctc cgtccggtca gtgacgcgcg cgcccgcgag
      421 cgtctcggtc accatgcggt agagcaccgg cgtttccagt gaccagggct tcggcgcggc
      481 gacgtggagg gtctgggtcg cctcccgtgt cgctccggcc tcgacccgca tcggggagcc
      541 ggtcgccgcc gccacccgtc ttccgtccgg gtcgagcacc gtggaccgca cccggacact
      601 cgccgccgac cctgcgtcgt tcgccacctc ggtgcggacg gtgagcgccg ccccgccggg
      661 gcccatctcg ctcgtgacga cggtccccca ctggggcacg tggagcgagt cggtcttcac
      721 gagccagacg tggcggtaga tccccgctcc ctcgtagaac cacccttcat gctcggtcgc
      781 gtcgacccgg aggaccagga tgttcgtccc gccgtaggtg acgaggtcgg acacgtcgta
      841 ccggaacggc gcgtagccgc tcaggttgcg cccgaggtag tgcccgttga gcgccgcggt
      901 gcagtcgcgg aagaccccgt cgaactcgag cgagatccgc cgccccgagt cggcggcggg
      961 gatgtcgaac gtcctccggt accagccgat gctggtgtcg gggtacttcc ggccgagcgg
     1021 cttcgagccg tggcccactt cgtcggggtc gttgatgaag gggaggtcga ccgcccagtc
     1081 gtgcgggagg tccacctgcg accaggcgct gtcgtcgaaa tccgcagcgg aggggccgaa
     1141 gaactctccc gccttggcaa agaggtcgcc ggtgccgtag ccgaagtcgc gggccggatc
     1201 gtccgcattg ccgagatgga accgccagcc aaggtcgagg aggaggcgat cgcgcgccgt
     1261 gggcggcgcg cctgcggagg ccggctcgcg gaggtccgcc ggtcgcaccg ggagcgtgag
     1321 cgcggccgct gaaagcgccg caccggcctg caggatatcc cggcgtgtca tcgtcacggg
     1381 tgcatcctcg tcgcggagag cgtcacccgc acgaacgccc cgccatcggg tcgattctgc
     1441 gcggcgaccg tgccgcgctg ctgctccacc agccggcgcg cgatggcgag gcccagcccg
     1501 gtgccgcctg gccgccggcc ggcgaacggg tggaagaggt gggggagaag gtcgtcgggg
     1561 aagccgggcc ccgagtcggc gacctcgcac tcgaccagcc cgccgccacc ctgctggcgg
     1621 atcgtgacgc ggacctcccc gccggccggg gaatgctgca ccgcgttggt gatcaggttc
     1681 acgaagacct ggatcagccg gcttcggtcc atcaccgccg tcggcaccgg atcgccggcg
     1741 tcgatccgga tgcggacccc ggcggcggtg gcgagcggcg cgcaggccac cgccgcgctc
     1801 cggatcaccg ccgcggcgtc gcccggctgg aggtcggggt cggtcggctt gccgtactcg
     1861 agcaggtccc gcgtgagcgt ggtcagccgg gtgacggcgc tcttgaggtc gtcgaagatg
     1921 tcggcgcggc gcgcggcggc atcggcctcc gccaccgcga gggtctgcag gccggatgag
     1981 atggccatga gcggattgcg gacctcgtgc gccacgccac ccaccagctc gcccatctcg
     2041 gccatcgcct cggcgcggcg gagcgattcg ttggcggcga gcgtctcgcg gcgcgcctcc
     2101 tcgatgacaa cgatcagcag gaagacggag atggtgatcc ccaggaaatc cgagaccgcg
     2161 agcccggcga tgacgcccca ggtcttgtcg gcgaggaacg ggtagtcgag ctggtggagt
     2221 ccctgcagcg cgaagagcgc cgtcagcacg ccccggacac cgatggccgg cccccgccag
     2281 aacgtcacgg cgatccagag ccccagcgcc cccttgatga gcgaatcggg ggactcgcgt
     2341 gcgaagaagc cgcccgtcac ctgcggcccg atgatgctcc aggccaccag cgccgtgaag
     2401 gcggcaccca gcgccagcca cgggatccgc cgccgcacga agtcatagca cccgcccacc
     2461 catgccacgg tgccgaagct cccagccgcg gcggcggcga cgtacgccgc cgccgccccc
     2521 cggccctgcg cgaggtgctg cggcagctgc agcaggagct cggccacgcc gaacgtggcc
     2581 gcgaccgcga gcagggccac ataccggcgg cgcaggatcg tctgccaggc atagaagaag
     2641 gagaacgcca gcgcgaggtt ggtggcgccc gagaggatcg tggcgacggc gatcaggttc
     2701 acgggcgggc tcccactccc tctggcgcga agggtgacgc tctagtgtac cgggccgggg
     2761 ccgatcgggc tagacggccc ggtcgatgcg acgtcccggc atcgcggggc atctggaagg
     2821 ggacccgtat cgagcgatcc aatgcgcctt gccgcctcgg cctcgttgtt cctcctgctc
     2881 gtcggcccga catccacgcc ccggaccggc actccctcca ttccggatgc cgatcgctca
     2941 cggatcgccg aggcgttccg ggtcgccgat ggcgtcggtg accggatctg gccgggatgg
     3001 cacgcggtgc catttgcgat cctgctgatc accgactcca ccgagttcct gatccaccat
     3061 ccccacccca cgtcggattt caccccggcg gggaccgatc ctgccctcgg accgatctgg
     3121 gtccgcccgc ggcggctccc cgtgacgctg ctcgccacgg ctcccttcgt cggtgggatc
     3181 ccgacgatcg ccgtcgggcg ggcggaggcg gtagggcaga gctcggtgga atgggtactg
     3241 acgctgctgc acgagcattt ccatcagctg caatacggcc agccgggata ttacgccgcg
     3301 gtcgccgccc tcggcctggc ccatggcgac tcgaccggga tgtggatgct caactacccg
     3361 ttcccctacg actcggtgcc ggtggtgcga gccatcgatt cgctggcggc agcgctggtc
     3421 acggtgctcg atgccgcgcg tccgggcgcc gatgcgccgg cccccag
//
LOCUS       DASUAP010000213         5587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_88000, whole genome shotgun sequence.
ACCESSION   DASUAP010000213 DASUAP010000000
VERSION     DASUAP010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88000"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFU45_05535"
     CDS             complement(<1..333)
                     /locus_tag="VFU45_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5358556.1"
                     /translation="MVRPTEVPVRARTLLVIDDEPPIRRAVANALRDVAERVVEASTG
                     AEGTELAAAERPELVVLDLGLPDLPGVEVCREIRRWSPTPILVLTARHDESEKVLLFN
                     SGADDYVTK"
     gene            complement(333..1331)
                     /locus_tag="VFU45_05540"
     CDS             complement(333..1331)
                     /locus_tag="VFU45_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5358557.1"
                     /translation="MNDRNVPRLAVIGTGEVGRGWAALAVAHGWTVSIFDTDSASLHD
                     AHDDIAGRVDRMAIAERADQHAADRGLAELSAGRSLLQAVAEADWIIEATPDDLGSKQ
                     RLLEQVEQVARLKAVLTSSSSLLHASELCGRLRRPERLLVAHPMVPVEMLPVVEVVPG
                     PRTDSVCTDDVCFWLRQLGRVPVLVRKEVPGHVLGRIQAAVWREVAQLVLDGVVDVRD
                     LDAALGLGPAVAWAAAGPALTHCLGARNRSISISLAAMLTGLESWWPTLARWEQLGSD
                     DRQRLVRALEKAYGARLPELRQERDDRLFRVLDAVEDAITHTEDDGVRLVQPPSAS"
     gene            1472..4258
                     /locus_tag="VFU45_05545"
     CDS             1472..4258
                     /locus_tag="VFU45_05545"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5358558.1"
                     /translation="MSRAIRTFLAIALAIALPSSTVAQKPVRRAAAPATAGLTKGPTV
                     EGITQYTLGNGLRVLLFPDASQPTATVNITYLVGSRNEGYGEKGMAHLLEHLMFKGSP
                     GHKNIPQELTEHGASPNGTTWFDRTNYFETFASTDVNLSWALDLEADRMVHSYIAEKD
                     LKSEFSVVRNEFESGENDPSSVLEERLMSTAYLWHNYGNSTIGDRADIEQVPIGRLQA
                     FYHKYYEPDNAVLVVAGKFDPARALQLVAQKFGRIPRPARTGDMKIWPTYTAEPVQDG
                     ERSVTLRRVGDVGWVMVGWHVPAGSDPDFPAVAVLARVLGDTPSGRFYKALVETKKAS
                     TEYAFAYQLREPGMLIAAAQLTAPDSAASASAALQDAVSALATDPPSAAEVDRAKTAI
                     VKNIDLTLNNSQRAALSLTEWSGLGDWRLLFVYRDLVKQVKPADVARVAAAYLKRSNS
                     TWGTFVPTKAPDRATVPWSPDVAALVDNYHGDSARSVGEAFDPSPRNIDARTTRRTLK
                     DGFQLALLPKKTRGATVNAAITLRFGSLDAVRGQAAVADLAADMLSRGTTTRTRQQIK
                     DEWDRLQSQVSISGGPTSANIRIESTRDNLAATLRLLGDVLHNPSFDAKEFDQLKAEN
                     LTGIEEQRSEPTSLGSIAFGKALKPYPADDPRAVLSADEQKAAYTAATLDEVRHFYQA
                     FYGADHGQMSIVGDFDTASTARLVDSLFGDWKSSSVFARVPSEYNDVPGGKTVIKTPD
                     KANAFFLSGMNLAVKDDDPSYPALVLGNYMLGGGFLNSRLAERIRQKEGISYGVGSGL
                     VASSLDRAGLFEAYAIYAPENVGRLEAAYQEEVARALKDGFTQDELDKARQGWLQQNE
                     LMRSRDAALAGTLTSDLFVGRTMAYDSTLEARVRGLTAADVNAAFRRFIDPAKFITVE
                     AGDFDKKPAQPVHP"
     gene            complement(4266..4466)
                     /locus_tag="VFU45_05550"
     CDS             complement(4266..4466)
                     /locus_tag="VFU45_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358559.1"
                     /translation="MSGGIGVQRIDGGRDRSEWHGELRAAHRWGAANELAVYGGITNS
                     AAAYTTGAFRSGTAGMAVRSGL"
     gene            4682..5062
                     /locus_tag="VFU45_05555"
     CDS             4682..5062
                     /locus_tag="VFU45_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5358560.1"
                     /translation="MTDPATTDTRALGRSLIDAFGKAWEKAKVDAMLEVFTPGAAFLE
                     SPFAAPIQGIEGIRGYWQDVPYHQSEITFSSGEIYAAGPWFSTEFKVVYRRRRTGEWV
                     DARGAIFCETDGEKISEMRMYWIR"
     gene            complement(5176..>5587)
                     /locus_tag="VFU45_05560"
     CDS             complement(5176..>5587)
                     /locus_tag="VFU45_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HEU5358561.1"
                     /translation="TLVTSGQPGPRHLEALKSAGAQVILDLRDPMEARPFDEPAAARA
                     LGLEYVNIPVASGAIDDALLERILEVARRSAERPTLVHCASGNRVTGPLLAYLMIDRG
                     MDEDNATELAMRMGLRSPEMLEWGVEYARRVIEG"
BASE COUNT          864 a         2031 c         1911 g          781 t
ORIGIN      
        1 cttggtcacg tagtcgtcgg cgccgctgtt gaagagcagg accttctccg actcgtcgtg
       61 ccgggcggtg aggaccagga tcggcgtcgg cgaccaccgg cggatctcgc ggcagacctc
      121 gacgcctggc aggtccggca ggcccaggtc gagcaccacg agctccgggc gctccgccgc
      181 ggcgagctcc gtcccttccg cgccggtcga cgcctccacc acgcgctcgg cgacgtcgcg
      241 aagggcgttg gccaccgccc ggcggatggg gggttcgtcg tcgatgacga gcagggttcg
      301 ggcgcggacg ggcacctcgg tcggtcggac catcagctcg ccgacggtgg ctgcaccagg
      361 cgcacgccgt cgtcctcggt gtgggtgatg gcgtcctcca ccgcgtcgag gacgcggaag
      421 agccggtcgt cgcgttcctg gcggagttcc ggcaggcggg ccccgtacgc cttctcgagc
      481 gcgcgcacca gccgctggcg gtcgtcgctc cccagctgtt cccaccgggc cagcgtgggc
      541 caccacgact cgagcccggt gagcatcgcc gccagcgaga tgctgatgct gcgattgcgg
      601 gccccgaggc agtgtgtcag cgccggcccc gcggcggccc acgccacggc aggcccgagc
      661 ccgagggcgg cgtcgaggtc gcggacgtcc accacgccgt cgagcaccag ctgggcgacc
      721 tcgcgccaga cggcggcctg gatccggccc agcacgtgac ccgggacctc cttcctgacc
      781 agcaccggca cccggcccag ttggcgcagc cagaagcaga cgtcgtcggt gcacactgag
      841 tcggtgcggg ggccggggac cacctcgacg accggcagca tctcgaccgg taccatcgga
      901 tgggccacca gcaaccgttc ggggcggcgc agccggccgc agagctcgct ggcgtggagc
      961 aggctgctcg aactggtgag caccgccttg agccgggcca cctgctccac ctgctcgagg
     1021 aggcgctgct tggacccgag gtcgtcgggg gtggcctcga tgatccagtc ggcctccgcc
     1081 accgcctgca ggaggctccg tccggcggaa agctccgcca gcccgcggtc agcggcgtgc
     1141 tggtcggcgc gctccgcgat ggccatccgg tcgacccgcc cggcgatgtc gtcgtgggcg
     1201 tcgtggagcg aggcggagtc ggtgtcgaag atcgacacgg tccacccgtg cgccacggcg
     1261 agcgcggccc agccccggcc gacctccccg gtgccgatga ccgcaaggcg tggaacattc
     1321 cggtcgttca tccatcatct cccggccggc ggaagcggaa ctgcgacgtt gcaagatggc
     1381 aggggccgcc gtccgccgcc actccgggga gccttgcgcg cggcggcctc ggggttacga
     1441 tgggacttcc ccgacgaccc ttcgaggatc catgtcccgc gccatccgca cgttcctcgc
     1501 catcgcgctc gcgatcgcgc tcccgtcgtc caccgtcgcc cagaagcccg tccgccgcgc
     1561 cgctgcgccg gccacggccg gcctcaccaa gggcccgacg gtcgagggca tcacccagta
     1621 caccctcggc aacggcctcc gggtgctgct cttcccggac gcatcccagc cgaccgccac
     1681 ggtcaacatc acctacctcg tcggctcccg gaacgagggc tacggcgaaa aggggatggc
     1741 gcacctcctc gaacacctga tgttcaaggg ctcgcccggg cacaagaaca tcccgcagga
     1801 actgaccgag cacggcgcca gccccaacgg caccacctgg ttcgaccgga ccaactactt
     1861 cgagaccttc gcctcgaccg acgtcaacct gtcgtgggcg ctcgacctcg aggccgaccg
     1921 gatggtccac agctacatcg cggagaagga cctcaagtcg gagttctccg tggtgcgcaa
     1981 cgagttcgag tcgggtgaga acgacccgtc gagcgtcctc gaggagcggc tgatgtcgac
     2041 cgcgtacctc tggcacaact acggcaactc caccatcggc gaccgtgccg acatcgagca
     2101 ggtgccgatc gggcggctgc aggcgttcta ccacaagtat tacgagcccg acaacgcagt
     2161 gctcgtcgtc gccgggaagt tcgacccggc gcgcgcgctc cagctggtgg cgcagaagtt
     2221 cggccggatc ccgcggccgg cccgcaccgg cgacatgaag atctggccga cctacaccgc
     2281 cgagccggtg caggacggcg agcggagcgt gacgctgcgc cgggtgggcg acgtcggctg
     2341 ggtgatggtc ggctggcacg tgccggcggg ctcggacccc gacttcccgg cggtggcggt
     2401 gctggcccgc gtgctgggcg acaccccctc cgggcggttc tacaaggcgc tggtcgagac
     2461 caagaaggcg tcgaccgagt acgccttcgc gtaccagctc agggagccgg ggatgctcat
     2521 cgccgcggcc cagctcactg cgcccgattc ggcggcgtcc gcctcggccg ccctccagga
     2581 cgcggtgtcc gcgctcgcca ccgacccgcc gagcgcggcc gaggtcgatc gggccaagac
     2641 ggccatcgtc aagaacatcg atctcacgct caacaactcg cagcgtgcgg cgctgagcct
     2701 caccgaatgg tcgggtctcg gcgactggcg gctgctcttc gtctatcgcg acctggtcaa
     2761 gcaggtcaag cccgccgacg tggcccgggt ggcggcggcg tacctcaagc ggtcgaactc
     2821 cacctggggc accttcgtgc cgaccaaggc gcccgaccgc gcgacggtgc cctggtcgcc
     2881 cgacgtggcg gcgctggtgg acaactacca cggcgacagc gcccgctcgg tgggcgaggc
     2941 gttcgacccc tcgccccgga acatcgacgc ccgcaccacg cggcgcacgc tcaaggacgg
     3001 gttccagctg gcgctcctcc ccaagaagac ccgcggcgcc acggtcaatg cggccatcac
     3061 gctccggttc ggctcgctcg atgcggtgcg gggtcaggcc gccgtggccg acctcgccgc
     3121 cgacatgctc tcccgcggca ccaccacccg cacccgccag cagatcaagg acgagtggga
     3181 ccggctgcag tcgcaggtgt cgatcagcgg cgggccgacc agcgcgaaca tccggatcga
     3241 gagcacccgc gacaacctgg ccgccaccct ccggctcctg ggcgacgtgc tgcacaaccc
     3301 gtcgttcgat gccaaggagt tcgaccagct caaggccgag aacctgaccg ggatcgagga
     3361 acagcgctcc gaaccgacgt cgctcgggtc catcgcgttc ggcaaggcgc tcaagcccta
     3421 cccggccgac gacccgcgcg ccgtcctgag cgccgacgag cagaaggcgg cctacaccgc
     3481 cgcgacgctc gatgaggtgc ggcacttcta ccaggcgttc tacggcgccg accacgggca
     3541 gatgtccatc gtgggcgact tcgacaccgc ctcgaccgcg cggctggtcg actcgctctt
     3601 cggcgactgg aagagctcgt cggtctttgc ccgggtgccg agcgaataca atgacgtgcc
     3661 cggcggcaag accgtcatca agacgccgga caaggccaac gcgttcttcc tctcggggat
     3721 gaacctggcg gtcaaggatg acgacccgtc ctacccggcg ctcgtgctcg gcaactacat
     3781 gctgggcggc gggttcctca actcgcggct ggcggagcgc atccggcaga aggaagggat
     3841 cagctacggc gtggggtcgg ggctcgtcgc ctcgtcgctc gaccgggccg ggctctttga
     3901 ggcgtacgcc atctatgcgc cggagaacgt gggacggctc gaggcggcgt accaggagga
     3961 ggtggcccgg gccctcaagg acgggttcac ccaggatgag ctggacaagg cccggcaggg
     4021 gtggctgcag cagaacgagc tgatgcggtc gcgggacgcg gcactcgccg ggacgctcac
     4081 cagcgacctg ttcgtgggcc gcacgatggc ctacgactcg acgctggagg ccagggtccg
     4141 cgggctcact gccgcggacg tgaacgccgc gttccggcgc ttcatcgacc ccgccaagtt
     4201 catcaccgtc gaggcagggg acttcgacaa gaagcccgcc cagccggtgc acccctagcg
     4261 ggccgctaca gccccgaccg gaccgccatc ccggcggtcc ccgagcggaa ggcgccggtg
     4321 gtgtaggcgg cggcggagtt ggtgatcccg ccgtacacgg ccagttcgtt ggccgctccc
     4381 caccggtgcg ccgcccggag ttcgccgtgc cattccgagc ggtccctccc gccgtcgatc
     4441 cgctgcacgc cgatccctcc gctcaccctc cgcgccaggg cgcggctctc gcgcgcccaa
     4501 ccgcctgcgc ctcgacggtg acgaacggcg ccggcaggcc cggggaaggg tagggagggc
     4561 catcgccaag aacttcccaa gatgccggcg ccggggcagg ccggacgtgg cgaaccgggg
     4621 cccgtcggct atgttccacg ttgtctttgg accgttcgac cgacaccctc tggagtcagc
     4681 cgtgaccgac cccgccacca ccgacacccg cgccctgggc cgctcgctca tcgacgcctt
     4741 cgggaaggcc tgggagaagg ccaaggtgga tgcgatgctc gaggtgttca cccccggcgc
     4801 cgcattcctc gaatccccgt tcgcggcgcc catccagggg atcgagggca tccgcgggta
     4861 ctggcaggac gtgccgtatc accagtcgga gatcaccttc agttccggtg agatctacgc
     4921 cgccggcccc tggttctcca ccgagttcaa ggtcgtctac cgccgccgcc gcaccggcga
     4981 atgggtggac gcccgcggcg ccatcttctg cgagaccgac ggcgagaaga tcagcgagat
     5041 gaggatgtat tggatacggt gaacggagaa cagggaaaag ggaaaaggga agagggaaaa
     5101 gggaacgggc catcgatcga gatctcgatc ggcggcccgt tcaccgttca ccgttgtccg
     5161 ttgaccgttc cccgttcacc cctcgatcac cctccgcgca tactccaccc cccattccag
     5221 catctccggc gaccggagcc ccatccgcat cgccagttcg gtggcgttgt cttcgtccat
     5281 cccccggtcg atcatcaggt aggccaggag cggtccggtg acccggttgc cgctggcgca
     5341 gtggaccagc gtcgggcgtt cggcgctccg ccgggccacc tcgaggatcc gctcgaggag
     5401 ggcgtcgtcg atcgcgccgg aggccaccgg gatgttgacg tactcgaggc cgagcgcgcg
     5461 agcggccgcc ggttcgtcga agggacgcgc ctccatcggg tcgcgcaggt cgaggatcac
     5521 ctgggcgccg gccgacttga gcgcctccag gtggcgcggg cccggctggc cgctcgtgac
     5581 gagcgtg
//
LOCUS       DASUAP010000214         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_919099, whole genome shotgun sequence.
ACCESSION   DASUAP010000214 DASUAP010000000
VERSION     DASUAP010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919099"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..815
                     /gene="larB"
                     /locus_tag="VFU45_05565"
     CDS             45..815
                     /gene="larB"
                     /locus_tag="VFU45_05565"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEU5358562.1"
                     /translation="MTPELLRGLLAEVAGGRLSPADALDRLRDFPTAQLEFAQVDHHR
                     ELRQGQPEVIFCERKTPAQVTAIAEHLAAASGGFLGTRAGADHAAAVRERFPTVEWNE
                     LGRTLWLRGPGGNGAPADGPVTLVVCAGTSDLPVAEEAWVTLQALGHRARKLVDVGVA
                     GIHRLLAAGDQLRDASVVIVVAGMEGALPSVVGGLVGVPVIAVPTSVGYGAAFGGVAA
                     LLGMLNSCAAGVTVVNIDNGFGAAMAASRIHRRAAVGR"
     gene            812..2212
                     /locus_tag="VFU45_05570"
     CDS             812..2212
                     /locus_tag="VFU45_05570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5358563.1"
                     /translation="MNPLWLVPAVGAGFAGAWWAGRLGAPGGRPSPGAVLPAPAVRWL
                     REAQQALGVWAVARDAVSEDTLPERSVDEGRLSSRDEETVLGRLRLAASGNGSTVERL
                     ECGVLLADVLGSRVAGLLFPPGIAPTRIDAAREDLRVFLEALAYRSVSEGWSKERSLL
                     AETPGTVALALAFELERSLNTEALVAAVDGTGVRVVGASLRSDRRLLNLSLPAESPLA
                     RVARGELAQLHTTHDPAGNVVADRRHRDLANLVLPLEHDGRVVGAAAISLPHNHDLGP
                     TQMSGVANALRLAAPRLSLALATEAVRATATTDPLTGLVNRRGLQVAMNRVGVDSGAL
                     IYADLDHFKVLNDTLGHPAGDAALVHFAAVLQEQVRGSDVAARVGGEEFAVWAPGAGL
                     ALGAQVAERIRSALADGVWMWQGHRHALTASFGVAAWPETSPSRQNLELQADAALYVS
                     KQQGRNRVSLAPTALS"
     gene            2248..2607
                     /gene="ssrA"
                     /locus_tag="VFU45_05575"
     tmRNA           2248..2607
                     /gene="ssrA"
                     /locus_tag="VFU45_05575"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          332 a          894 c          999 g          383 t
ORIGIN      
        1 tacctcgaag gccaccaggg ccttccggcc gtcgcgcagg tggcgtgacc ccggaactcc
       61 tccggggcct gctggcggag gtggccggcg gccgcctgtc ccccgctgac gcgctggacc
      121 gcctgcgcga ctttcccacc gcccagctcg agttcgccca ggtggatcac catcgggagc
      181 tccggcaggg gcagcccgag gtgatcttct gcgagcgcaa gaccccggcc caggtcaccg
      241 cgatcgccga gcacctcgcg gccgcatccg gtggtttcct cggcacccgc gcgggcgctg
      301 accacgccgc agccgtgcgg gagcggttcc cgacggtcga gtggaatgag ctcgggcgga
      361 cgctctggct gcgcggaccc ggcgggaacg gcgccccggc cgacgggccg gtgacgcttg
      421 tcgtctgcgc cggcaccagc gacctgccgg tggcggagga ggcgtgggtg acgctccagg
      481 cgctcgggca ccgcgcccgg aagctggtgg acgtcggcgt cgccgggatc caccgcctgc
      541 tcgccgcggg agaccagctt cgcgacgcat cggtggtgat cgtcgtggcg gggatggaag
      601 gtgcgttgcc gagcgtggtg ggcgggctcg tgggagtgcc ggtcatcgcg gtgccgacga
      661 gcgtgggcta cggggccgcc ttcggcgggg tcgctgcact cctcgggatg ctcaattcct
      721 gcgccgcggg cgtcaccgtg gtcaacatcg acaacggatt cggcgccgcg atggcggcga
      781 gccgcatcca tcgccgggcg gcggtgggcc ggtgaatccg ctctggctcg ttccggcggt
      841 cggcgccggg ttcgccggcg cgtggtgggc cggtcgcctg ggggcaccgg gcgggcgtcc
      901 gtctcctggc gcggtgctgc cggcgccggc ggtccggtgg ctccgcgagg cccagcaggc
      961 gctcggcgtc tgggccgtgg cccgcgatgc ggtgtcggag gatacgctcc ccgagcgatc
     1021 ggtcgacgag gggcggctca gttcccgcga tgaggaaacg gtgctgggac ggctccggct
     1081 ggccgcctcc gggaatggct cgaccgtgga gcggctggag tgcggcgtgc tgctggccga
     1141 cgtcctcggc agcagggtgg cggggctcct cttccccccc gggatcgcgc cgacccggat
     1201 cgatgcggcg cgcgaggacc tccgggtctt cctcgaggcg ctcgcgtacc ggagcgtctc
     1261 agaagggtgg tccaaggaac gttcgctgct tgcggagacg ccgggcaccg tggcgcttgc
     1321 gctcgccttc gagctggagc gatcgctcaa caccgaggcg ctggtggccg cggtcgacgg
     1381 cactggtgtc cgggtggtcg gagcctcgct ccggtcggat cgccggctcc tcaatctgtc
     1441 gctcccggcg gagagcccgc tggcccgggt ggccaggggg gaactggcgc aactgcacac
     1501 cacgcacgat ccggccggca acgtggtggc cgaccggcgg caccgggacc tcgcaaacct
     1561 ggtcctcccg ctggagcacg acggccgcgt ggtcggtgcc gccgcgatca gcctaccgca
     1621 caaccacgac ctggggccga cgcagatgag cggggtcgcc aatgcgctgc ggctggctgc
     1681 cccgcgtctc tcgctggccc tggccaccga ggcggtgagg gccacggcga ccaccgaccc
     1741 gctgaccggc ctggtcaatc gtcggggcct ccaggtggcc atgaaccgcg tcggggtcga
     1801 ttccggcgcc ctgatctacg ccgacctcga ccacttcaag gtcctcaacg atacgctggg
     1861 ccaccccgcg ggagatgccg cgctggtgca cttcgccgca gtgctgcagg aacaggtccg
     1921 cggttccgac gtggcggcgc gggtcggggg ggaggaattc gcggtctggg ccccgggggc
     1981 gggcctggcg ctgggcgcgc aggtggcgga gcggatccgg agtgcgctcg ccgacggggt
     2041 gtggatgtgg caggggcacc ggcacgcgct caccgcctcg ttcggcgtgg cggcgtggcc
     2101 cgaaacgagc ccgagccggc agaacctcga actccaggca gatgccgcgc tctacgtctc
     2161 gaagcagcag ggccggaacc gggtgtcgct ggcgccgacc gcgctgtctt gagtcggggg
     2221 cctcgggagg gtatccttcg gtacgtcggg ggcgatcggc ttcgacgggt ttggtgaacg
     2281 aatcgctgcg tgcccaggtc caggtccctg gctaaaccac ttggaatgct tcatctgcca
     2341 actccaactt ggcaatggct gcgtaagctg agctaagctc gcttaagcac ctccgatgaa
     2401 cggagcctgt ccggggcgcc gccatcggat cgaaaatccg ggctggcgaa cgccgtcgcc
     2461 ctgccggcgc aagcgagacc tcaggggctg gtccccggca cggccggctg tgcgccggac
     2521 cggggagacg cgaagagcac ggcctacgca cgtaggcgcg gttccggaag caagctcgga
     2581 ccagggttcg attccctgcg cctccatc
//
LOCUS       DASUAP010000215         6683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_265094, whole genome shotgun sequence.
ACCESSION   DASUAP010000215 DASUAP010000000
VERSION     DASUAP010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265094"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFU45_05580"
     CDS             <1..256
                     /locus_tag="VFU45_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5358564.1"
                     /translation="RENFLYMRPEWPLDDDSPLLRGGVIDSVGVIELVAHLEAAYQFT
                     IDESDITEANLGTLGAIGRFVAERHRDGAAGAGEWPRRTA"
     gene            235..1353
                     /locus_tag="VFU45_05585"
     CDS             235..1353
                     /locus_tag="VFU45_05585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5358565.1"
                     /translation="MAAPHRLTAGAGDPGVETVRSFRAADIPEVVALRRRVFRYSERN
                     TDAALTEYFERMFCRPRSAVDGLPSLVHVDGQGRVLGFLGVFPRQMRFRDERILVAVA
                     TQLMVAPESRGLAGRALVRAFLRGPQDLSFSDTANDAARRLWVSLGANVSPAPSLSWT
                     QVLRPWRHLGAEASHDGAGLVVRAALYAARPLLSVADLLLTRRPSARPVALTEPFDPT
                     RVAAVAEDVLGGYGLRPVYDAETLAWLLAQAAEKREYGTLDGRIVRGDDGSVAGWFLY
                     YRNRTGMSQVLQVAAQPRDRGLVLDAMLEHARQHGVVALAGPLDPGTIHEAAIRRSTL
                     RHEGPWVLFSSQRRDLMQAIAGGDAYLSRLEGEWWMSF"
     gene            1563..2237
                     /locus_tag="VFU45_05590"
     CDS             1563..2237
                     /locus_tag="VFU45_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEU5358566.1"
                     /translation="MVRWLAPAVVALSAAACAAGGRSAASAAVRPEPQQGEAAAVARA
                     RADSVRHPYTQADIDFMSGMIGHHSQAIVMAGWARSHGASSGVLVLCQRIINAQRDEI
                     RIMQQWLRERNQPVPAQDTTGRGMMMMGGQLHQMLMPGMLTDAQMDTLNASRGPEFDQ
                     RFLQYMIQHHTGATSMVSELFSTPGAAEDELTFKLASDINVDQTTEITRMHKMLTSLV
                     LGVPVP"
     gene            2405..4300
                     /locus_tag="VFU45_05595"
     CDS             2405..4300
                     /locus_tag="VFU45_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358567.1"
                     /translation="MSTPAPTPKYDMSTKAPSPDPRVGLKAGLNDAGQAAWNMQLLSN
                     TQSTDSAFRLATHSDLAFLGKYAIQGTYNGFIFWDISNPSAPVQAEAYQCPASQSDVS
                     VYKNLLFASGEGLSGRLDCGAEGVKDTVSKERLRGLRIFDISDIHHPKNVGNVQTCRG
                     SHTHTLLVDPKDPDNVYIYISGSAPVRSPNELAGCSAAPPDQDSTSALFRIEVIKVPL
                     AHPEQAAIVSSPRIFNDLVAPPHHGLAPDDIKQLAAAKQRGAFIVDILGNQQVLPDAF
                     ANRMLDSVVKSHNRTGAPTAADSTELRDGLPGLIAKLLGQRNTKPTGPTQCHDITVDP
                     AIGLAGGACEGYGLLLDIRDPAHPVRIDAVSDSNFSYWHSATFNNDGTKVLFSDEWGG
                     GGAPKCRATDPKEWGADAIFTLADNKMHFQSYYKLPAPQTDKENCVAHNGSLIPIPGR
                     DVMVQAWYQGGVSVFDWTDAAHPKEIAFFDRGPVDSTHMVGAGSWSAYWYNGVIVSSE
                     IARGLDIFALQPSGFISQNELDAAKSVHFDEFNTQGQPKFWWPASFALSGAYVDQLER
                     SHGLSSTRVRAVRKDLAAAERASGSARTLALTQLASQLDGYAAGSSEPAKVHTLAQSV
                     RDLAAAK"
     gene            complement(4642..>6683)
                     /locus_tag="VFU45_05600"
     CDS             complement(4642..>6683)
                     /locus_tag="VFU45_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU5358568.1"
                     /translation="DADAVFGMEYAQAEDDFNRVETNYINAMGRLAEADGESAIYQDL
                     RMKLFVVPDSLKAMYRRSPVWLRSLMNAFADGLNYYLYTHPEVRPRVIRHFEPWMALS
                     FTEGSIGGDIERVNVRDLGAFYGADAKDVPVETGDGEVFPEPKGSNGIAIAPSDTKDH
                     HALLWINPHTSFYFRSELQMSSDQGLDAYGAVTWGQFFIYQGFNPTAGWMHTSSGVDN
                     IDEFLETVSRKGGRWYYRHGGKEFPVRVRSVSVPYRTASGMASRTFTVYYTPHGPVIR
                     RAGDNWVTISLMQNPISALIQSYSRTKAKDYAAFKRILALHTNSSNNTIFADAEGNIA
                     YFHSNYIPRRDTSFDWTRPVDGSDPRTDYHGVLAVDQSPNLQNPASGWLYNSNNWPWS
                     AAGPSSPKREDYPKYVETGTEESTRGRHALRVLSEGKDFTMGSLTAAAFDTWLPSFAR
                     MLPPLFRAWDSLPDGDSLKAALAGPAALLKGWDDRWDSTSVATSVAVFWGNDIERKLY
                     RDAQAADMGVETYVVERATPSQLLQSLVAATTRLTADFGSWKTPWGTINRFQRINDSI
                     SPSFDDAGPSIPVPFTSAQWGSLASFGARPYPNTKKWYGSSGNSFVAVVEFGDSVRAR
                     AVTAGGESGDPRSPHFNDQAERYATGDLREVYFYPDQLTGHTERVYHPGEVRSEK"
BASE COUNT         1019 a         2353 c         2234 g         1077 t
ORIGIN      
        1 ccgggagaac ttcctctata tgcgccccga atggccgctc gacgacgact cccccctcct
       61 gcgtggcggg gtgatcgatt cggtgggggt catcgagctg gtggcgcacc tcgaggctgc
      121 ctaccagttc accatcgacg agagcgacat caccgaggcc aacctcggca ccctcggcgc
      181 gatcggccgc ttcgtggcgg agcggcaccg cgacggcgcc gccggagccg gtgaatggcc
      241 gcgccgcacc gcctgacggc cggggccggc gaccccggtg tggagacggt ccgctcgttc
      301 cgcgcggcgg acatccccga agtcgtggcg ctccgccgac gggtcttccg ttactccgag
      361 cggaacacgg atgcggcgct gacggaatac ttcgagcgga tgttctgccg accccgctcc
      421 gcggtcgacg ggctcccgtc gctggtacac gtggacggtc aggggcgcgt gctgggcttc
      481 ctgggggtgt tcccccgcca gatgcgtttc cgggatgagc ggatcctggt cgctgtcgcg
      541 acccagttga tggtggcccc cgagagccgg gggctcgcag gccgcgcgct ggtgcgggcg
      601 ttcctccgcg ggccgcagga cctgagcttt tccgataccg ccaacgacgc cgcccgccgg
      661 ctctgggtca gtctcggcgc caacgtgtcc ccggcgccga gcctctcgtg gacccaggtg
      721 ctgcgcccgt ggcgccacct tggcgcggag gcgtcccacg acggcgccgg gctggtggtg
      781 cgggccgcgc tctacgccgc acgcccgctc ctgtcggtcg cggacctcct gctcactcga
      841 cggccatcgg cccgacccgt ggcgctgacc gagccgttcg acccgaccag ggtggcggcg
      901 gtggccgagg atgtgctcgg cggctacggg ctccgtccgg tgtacgacgc ggagacgctg
      961 gcgtggctcc tcgcccaggc ggccgaaaag cgggagtacg gcacgctcga cggacggatc
     1021 gtgcgcggcg atgacggcag cgttgccgga tggttcctgt actaccggaa ccggaccggg
     1081 atgagccagg tactccaggt ggcggcgcag ccgcgggacc gggggctggt gctggacgcg
     1141 atgctggagc acgcgcggca gcatggggtg gtggcgctgg cgggcccgct cgatccgggg
     1201 acgatccacg aggcggccat ccgccgatcc acgctccggc acgagggccc gtgggtgttg
     1261 ttcagctccc agcggcgtga cctgatgcag gccatcgccg gtggcgacgc ctacctctcc
     1321 cggctggaag gagagtggtg gatgagcttc tgatgcccgc cggagcgccg taccttcccg
     1381 cagtcctgac attgcaatct gaaactttcc accccgctcc cgcgtctcgg tagggcaccg
     1441 agcatccatc gccgtccggc cgcttgccac tttgggccgc ggtgctacca tctagcggct
     1501 ggccgtaccc cgcatcagca ggatccgccc gaatccggga gatcgtccat atggaacgtc
     1561 cattggttcg ctggctcgcg ccggccgtgg tcgcgttgtc ggcggccgcc tgcgccgccg
     1621 ggggccgatc cgccgcttcc gctgccgtcc ggcccgagcc gcagcagggc gaggcggccg
     1681 ccgtcgcccg tgcgcgggcg gacagcgtcc ggcaccccta cactcaggcc gacatcgatt
     1741 tcatgagtgg gatgatcggc caccactccc aggccatcgt gatggccgga tgggccaggt
     1801 cccacggcgc gagttcgggc gtgctggtcc tctgccagcg gatcatcaat gcgcagcggg
     1861 acgagatccg gatcatgcag cagtggctgc gggagcggaa ccagccggtg ccggcgcagg
     1921 acaccaccgg gcgggggatg atgatgatgg gcggacagct gcaccagatg ctgatgcccg
     1981 gaatgctcac cgatgcccag atggacacgc tcaacgcgtc ccgcgggccg gagttcgacc
     2041 agcgcttcct gcagtacatg atccagcacc ataccggcgc cacctcgatg gtgagcgagc
     2101 tcttctcgac gcccggcgcg gcggaggacg agctgacgtt caagctcgcc tcggacatca
     2161 acgtggacca gacgaccgag atcacccgga tgcacaagat gctcacctcg ctcgtcctcg
     2221 gcgtcccggt cccgtgaccc gacttcaccc gagtctctgc aggtatcact cagccccatc
     2281 agaggaacac gtcatggcat ccgtgcatca cgttcgccgg tcgggacggt ggccgagcgc
     2341 cgcccgactc tccgccttcg cgctggccgc ggggctcctc tcggcgtgtg cgcgtccggg
     2401 cgcgatgagc actcccgccc cgacgccgaa gtacgacatg tccacgaagg cgccgagccc
     2461 cgatccgcgc gtggggctca aggccgggct caacgacgcc ggccaggccg cgtggaacat
     2521 gcagctcctc tcgaacaccc agtccaccga cagcgccttc cggctggcga cgcactccga
     2581 cctggcgttc ctggggaagt acgcgatcca gggcacctac aacggcttca tcttctggga
     2641 catcagcaac ccgtcggcac cggtccaggc cgaggcgtat cagtgcccgg cgtcgcagag
     2701 cgacgtgtcg gtgtacaaga acctgctctt cgcctcgggc gaggggctga gcggccggct
     2761 cgactgcggc gccgaagggg tcaaggacac cgtgagcaag gagcggctgc gggggctccg
     2821 gatcttcgac atctcggaca tccaccatcc gaagaacgtc gggaacgtgc agacctgccg
     2881 cggctcgcac acccacacgc tgctggtgga tccgaaggac cccgacaacg tgtacatcta
     2941 catctcgggg tcggcgccgg tgcgttcgcc gaacgagctg gccgggtgct ccgccgcgcc
     3001 gccggaccag gactcgacct ccgcgctctt ccgcatcgag gtcatcaagg tgcccctggc
     3061 gcacccggag caggcggcca tcgtctcctc gccgcggatc ttcaacgatc tcgtggcccc
     3121 gccgcaccac ggcctcgcgc cggatgacat caagcagctt gcggccgcca agcagcgcgg
     3181 cgccttcatc gtggacatcc tgggcaacca gcaggtgctg ccggacgcct tcgccaaccg
     3241 gatgctggac agcgtggtca agtcgcacaa ccggaccggc gcgccgaccg cggccgacag
     3301 caccgagctc cgcgacgggc ttcccggcct gatcgcgaag ctcctgggcc agcggaacac
     3361 caagcccacc ggcccgacgc agtgccacga catcaccgtg gacccggcca tcggcctggc
     3421 ggggggcgcc tgcgaggggt acgggctcct gctcgacatc cgcgacccgg cgcacccggt
     3481 gcggatcgac gcggtgtccg actcgaactt ctcctactgg cactcggcca cgttcaacaa
     3541 cgacggcacc aaggtcctct tctccgacga gtgggggggc ggcggcgcgc cgaagtgccg
     3601 cgccaccgac ccgaaggagt ggggcgccga cgccatcttc acgctggccg acaacaagat
     3661 gcatttccag agctactaca agctccccgc gccgcagacc gacaaggaga actgcgtggc
     3721 gcacaacggc tcgctcatcc cgatcccggg ccgggacgtg atggtgcagg cctggtacca
     3781 gggcggggtg tcggtgttcg actggaccga cgccgcgcac ccgaaggaga tcgccttttt
     3841 cgaccgcggg ccggtcgact ccacccacat ggtgggcgcg ggttcctggt cggcgtactg
     3901 gtacaacggc gtcatcgtga gctccgagat cgcccgcggg ctcgacatct tcgcgctcca
     3961 gccgagcggg ttcatctcgc agaacgagct ggatgccgcc aagagcgtgc acttcgacga
     4021 gttcaacacc cagggccagc cgaagttctg gtggccggcg tcgttcgcac tctcgggcgc
     4081 gtacgtggac cagctggagc ggtcgcacgg gctgagctcg acgcgggtcc gcgcagtgcg
     4141 gaaggacctg gccgcggccg agcgggcatc cgggtcggcg cggacgctgg cgctcaccca
     4201 gctggcatcg cagctcgatg gctatgcggc agggtccagc gagcccgcca aggtccacac
     4261 gctcgcccag tcggtgcggg acctcgccgc cgcaaagtga ccgcctgatg gcgacggaca
     4321 agaagaaggg gaccgccact cggcggcccc ctttttctac ctcacccccc aaccccctct
     4381 cccttacgga agagggggag gactgcgttc gatgccagac tcgaactcgc tgatcgtcac
     4441 ctttcacgtc taacctcacc ccccaacccc ctctccctta cgggagaggg ggagcactgc
     4501 gttcgatgcc agcctcgaac tcgcggatcg ccacctttca ccttccacct tccaccttcc
     4561 acctcccacc tcgtacctct caccgctccc ctctcacgcc cctctttcac tcttcgcgtc
     4621 ctggctcccc cttctcactt ctcacttttc acttctcact tcccccggat ggtacaccct
     4681 ttccgtgtgt cccgtgagct ggtccgggta gaagtacacc tcacggaggt cgccggtggc
     4741 gtagcgctcc gcctggtcgt tgaagtgcgg ggaccgggga tcgccgctct ccccacccgc
     4801 cgtcacggcg cgggcgcgga cgctgtcgcc gaactcgacg acggccacga agctgttccc
     4861 gctcgacccg taccacttct tcgtgttggg gtacggccgg gcgccgaacg aggcgagcga
     4921 accccactgg gccgaggtga acggcaccgg gatgctgggc ccggcgtcgt cgaacgaagg
     4981 actgatgctg tcgttgatcc gctggaagcg gttgatcgtc ccccagggcg tcttccagct
     5041 gccgaagtcc gcggtgaggc gcgtcgtcgc ggcgacgagg gactgcagga gctgggatgg
     5101 agtggcgcgc tcgacgacgt aggtctcgac ccccatgtcg gctgcctgcg cgtcccgata
     5161 gagcttccgt tcgatgtcgt tcccccagaa gaccgccacc gacgtggcca ccgacgtcga
     5221 atcccaccgg tcgtcccacc ccttaaggag ggcggccggc ccggcgagcg cggccttcag
     5281 cgaatcgccg tcggggagcg agtcccaggc ccgaaagagc gggggaagca tccgcgcgaa
     5341 cgaggggagc caggtgtcga acgccgccgc cgtgagcgac cccatcgtga aatcctttcc
     5401 ctccgacagc accctgagtg cgtggcgtcc ccgggtcgac tcttcggtcc cggtctccac
     5461 gtacttcggg tagtcctccc gcttcgggct gctcggcccc gccgccgacc agggccagtt
     5521 gttcgagttg tagagccacc cgctcgctgg gttctggagg ttcggcgact ggtccaccgc
     5581 caggacgccg tgatagtcgg tgcgcgggtc gctcccgtcc accggcctgg tccagtcgaa
     5641 cgaggtgtcg cgccgcggga tgtagttcga gtggaagtag gcgatgttcc cctcggcgtc
     5701 ggcgaagatg gtgttgttgg acgaattggt gtggagcgcc aggatcctct tgaacgcggc
     5761 gtaatccttt gccttggtgc ggctgtacga ctggatcagc gcgctgatcg ggttctgcat
     5821 cagggagatg gtgacccagt tgtctcccgc cctgcgtatg acggggccgt gcggcgtgta
     5881 gtacaccgtg aacgtgcggc tcgccatccc gctggcggtg cggtacggca ccgagacgct
     5941 ccgcacccgc accgggaact ccttcccgcc gtggcggtag taccagcggc cgcccttccg
     6001 ggacaccgtc tccaggaact cgtcgatgtt gtccaccccg ctcgaggtgt gcatccaccc
     6061 ggcggtcggg ttgaacccct ggtagatgaa gaactgcccc caggtcacgg cgccgtaggc
     6121 gtcgagcccc tggtcgctcg acatctggag ctcggagcgg aagtagaagg aggtgtgcgg
     6181 gttgatccag aggagggcgt ggtggtcctt cgtgtccgag ggcgcgatcg cgatcccgtt
     6241 cgatcctttg ggctcgggga agacctcgcc gtcgccggtc tcgaccggga cgtccttcgc
     6301 atcggcgcca tagaaggcgc cgaggtcgcg gacgttgacc cgctcgatgt cgccgccgat
     6361 cgaaccctcg gtgaaggaga gcgccatcca cggctcgaaa tgccggatga ccctcggccg
     6421 cacttcggga tgcgtgtaga ggtagtagtt gagaccgtcg gcaaaggcgt tcatcagcga
     6481 cctgagccac accgggctcc gacggtacat cgccttgagc gaatcgggga cgacgaagag
     6541 cttcatccgc aggtcctggt agatcgcgga ctccccgtcc gcctcagcca gccgccccat
     6601 cgcgttgatg tagttggtct cgacccggtt gaagtcgtcc tcggcctggg cgtactccat
     6661 cccgaacacc gcgtcggcgt cgg
//
LOCUS       DASUAP010000216         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_960417, whole genome shotgun sequence.
ACCESSION   DASUAP010000216 DASUAP010000000
VERSION     DASUAP010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960417"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="VFU45_05605"
     CDS             <1..1781
                     /locus_tag="VFU45_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358569.1"
                     /translation="VQASFTTDLRLMWPYPAGALGDLRFSLSADHRRAWVGVEGAAAQ
                     VVIGAWGGTLRVAPGKGTAVRVEVSGTGPLRIVVAAASDDADLERTLRALDQKRFEGI
                     QTQRTQHVQQLDGYGARLATGDPALDRAFDWAKCRMDSFLAGTPRVGRSLLAGYAGSR
                     PGWGDGRPGYAWYFGRDSCWTGFAMLAAGLREPVRDTLKFLSTTQDVTGKVLHEYTTS
                     GLVHYDAADSTPLYLLLAGRYASWTGDLATLDRHWEAIVRAYRFVRSTDRDDSGLIEN
                     RRVGHGWIEHGPLGGAAVTLYNVACWIAALEALEPVAEALGHPALAAEMAERARRGRA
                     TARRRLRVDGEWALGLLDDGTPQVHRTAMLSVPILLGLVPPAEAAAWYDAVAGDRFSA
                     PWGVRMIPTDDPLFDPAGYHLGSVWPLYTGWVSLAEWKGGRHDAALAHLAANARLASE
                     REKGAFDEVLHGTELRSAGICPDQAWSAAMVTLPAVEGLWGVGPRALDGAVSLTPWLP
                     PSWPEMGLERLRVGTTVLDLRIKRRPGQLVVHCRRTHGERIHVELAPRGLDHVRAISV
                     DGTDLPGGPSAAFDADGDHEVMFFEA"
     gene            complement(1943..>2178)
                     /locus_tag="VFU45_05610"
     CDS             complement(1943..>2178)
                     /locus_tag="VFU45_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5358570.1"
                     /translation="NDSRYGLQAGVFTRDVRRIWQAFERLEVGGVIANDYPTLRVDTY
                     PYGGVKDSGFGREGVRYAMEEMSELRTLVLAGK"
BASE COUNT          291 a          754 c          783 g          350 t
ORIGIN      
        1 cggtgcaggc gagcttcacg accgacctcc gcctgatgtg gccgtatccg gccggcgcgc
       61 tgggtgacct gcggttctcg ctcagcgcgg accatcgtcg ggcctgggtc ggcgttgaag
      121 gtgccgccgc ccaggtcgtg atcggcgcgt ggggcggcac gctgcgggtg gcgcccggga
      181 aagggacggc cgtacgggtc gaggtctccg gtaccgggcc gctccgcatc gtggtggcgg
      241 cggcgtcgga tgacgccgac ctggagcgca cgctccgtgc gctcgaccag aagcgattcg
      301 aggggatcca gacccagcgg acccagcacg tgcagcagct cgatggctac ggtgcccggc
      361 tcgcgaccgg cgaccccgcg ctcgaccgtg cattcgactg ggccaagtgc cggatggact
      421 ccttcctggc cggcacgccg cgggtggggc gctcgctgct ggccggctac gcggggtccc
      481 gccccggctg gggcgatggt cgcccggggt atgcgtggta cttcgggcgg gattcctgct
      541 ggaccggctt tgcgatgctg gccgcgggcc tgcgggagcc ggtgcgcgac acgctcaagt
      601 tcctctcgac cacgcaggac gtcacgggca aggtgctcca cgagtacacc acgagcggcc
      661 ttgtccacta cgatgccgcc gacagcacgc cgctctacct cctcctcgcc gggcggtacg
      721 cgtcgtggac cggcgacctt gccaccctcg accgtcactg ggaggccatc gtccgggcct
      781 accggttcgt gcgctcgacc gaccgggacg acagcggcct gatcgagaat cgccgggtag
      841 gccacggctg gatcgagcac ggcccgctgg gcggcgccgc ggtgacgctc tacaacgtcg
      901 cctgctggat cgcggcgctc gaggccttgg aaccggtggc cgaggcgctc ggccaccccg
      961 cgctcgccgc cgagatggcc gagcgggcgc ggcgaggccg ggccaccgcg cgccgccgcc
     1021 tccgggtcga tggcgagtgg gcgctcggat tgctggacga cggcacgccc caggtgcacc
     1081 gtacggcgat gctgagcgtg cccatcctcc tcgggctggt gccgccggcg gaggcggccg
     1141 cgtggtacga cgccgtggcg ggagaccggt tcagcgcgcc gtggggcgtg cggatgatcc
     1201 cgaccgacga cccgctgttc gatccggcgg ggtaccacct cgggtcggtg tggccgctct
     1261 acaccggttg ggtgagcttg gccgaatgga agggcgggcg ccacgatgcg gcgctggccc
     1321 acctggcggc gaacgctcgg ctcgcctcgg agcgcgaaaa gggggcgttc gacgaagtgc
     1381 tccacggcac cgagctccgc tctgcgggga tctgccccga ccaggcgtgg tccgcggcga
     1441 tggtgacgct gccggcggtg gaagggttgt ggggggtcgg accccgggcg ctcgacgggg
     1501 cggtgtcgct cacgccgtgg ctgccgccat cgtggccgga gatggggctt gagcggctgc
     1561 gggtcggcac caccgtgctc gatctcagga tcaagcggcg ccccggccag ctggtggtcc
     1621 attgccggcg gacccacggc gagcggatcc acgtcgaact cgcgccgcgc ggcctggacc
     1681 atgtgcgcgc gatctcggtg gacggcacgg accttccggg gggcccgagc gccgccttcg
     1741 acgccgacgg cgaccacgaa gtgatgttct tcgaagcgtg agacgtaaga cgtgagacgt
     1801 aagacgtgag agggacgagg tttgccgtga aaggtgcgga gtcggtgcct cgcgtctaag
     1861 cgcccaccgc tatacacatt tcactcttta cttttcactt ttcacttctt ccttctcctt
     1921 cttccttctc acttcttact tctcacttgc ccgccagcac cagcgtccgc aactcgctca
     1981 tctcttccat cgcgtagcgc accccctccc gcccgaaccc gctgtccttg acgccgccgt
     2041 atggataggt gtcgacccgg agggtcggat agtcattggc gatcacgccc ccgacctcga
     2101 gccgctcgaa tgcctgccag atccgccgta cgtcacgggt aaagaccccg gcttgcaggc
     2161 cgtaccgcga gtcgttca
//
LOCUS       DASUAP010000217         6006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_319626, whole genome shotgun sequence.
ACCESSION   DASUAP010000217 DASUAP010000000
VERSION     DASUAP010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319626"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1943
                     /locus_tag="VFU45_05615"
     CDS             <1..1943
                     /locus_tag="VFU45_05615"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEU5358571.1"
                     /translation="YAYGPPPVRLLLELHLTYADGRTEVVTSDSSWRTAPSPVLISEI
                     YDGETYDARLEQPGWDRPGFDAASWRHAIELPPVKVTIQPQAMPPIEETDSVRPIRRW
                     SPGAGVYVFDLGQNIAGWARLHVQGKAGTRVQMRFAEILDPDSSGNITQINLRSAKAT
                     DVYVLRGRGAETFTPHFTYHGFRYIEVTGYPGVPPMDAVIGVVAHTALPETGTFASSD
                     PMLDRLWQNVMWTQRANLYSVPTDCPQRDERMGWMGDAQVFWRTASYNMDMAAFGEKW
                     LADVRDGQTAPGCFPNFAPNFLGVLTCGAPGWADAGVIIPWTNWRQYGDTAELAAHWE
                     AMERYMAFIADSNAGYLWTRRKTVEFGDWLPAGSTTSWGQTNTDLISTAFWAYDASLM
                     AQMARAIGRGERAAHYDSLAGRIRTAFDRAFVLADGRVGAPFTNADSIHSDYTQTASV
                     LALRFGLVPDSQRAAVARHLVLDIRAHGGHLTTGFLGSSFILPVLSDAGQDDAAFELL
                     FQQSFPSWLYEVKAGATTTWERWNGDHGNPEMNSYSHYAFGAVGEWLYRYLAGIDEAP
                     DAVAFDRIVIHPRWTPQLDSVRAIYRSARGVITSGWVRQRDGSIVVSGRIPANTTGAV
                     MLPGWTAPKEIEAGAFRFVVRG"
     gene            complement(1968..3875)
                     /locus_tag="VFU45_05620"
     CDS             complement(1968..3875)
                     /locus_tag="VFU45_05620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5358572.1"
                     /translation="MRTAPPVDPLFLAFQSALAGRYSIDRELGRGGMGVVYLAREVHL
                     DRLVAIKLLPPERAAVPALRARFLREARLAAKLSHPNITPIHAVAETEGFVFFVMAYI
                     DGQTLTERVLERGPLPIADGTRMLREAAWALAHAHGQGLVHRDVKPDNILLEAATGRA
                     LVVDFGIAAAAGDAVEAGISGTPDFMSPEQALAGTIDARSDLYGLGATAFFAFAGRPP
                     FEGASATEVLAKQVTEPAPSLLSLGLTVPRKVAQLIERCLEKDPADRPASAEAVAEQL
                     SAALEQRRELPVALRVFAKRSGRIDGGGTILSFVALYAAIGAASIFLGPGWALATFLA
                     GATVPPLLYLVNAARKLRMQGYSHADVIPAFKREIETGREERGGRGAVPGRAERVLGP
                     IAKWSWISVGLSFGSLMALGGLGVTSDAVISWIVRALSVSGMVAGPAALGVLALRQRR
                     TDIDAELWAKVWAGRIGRLAFSLARPLVGKRAIGPAMTHRATELSLGMAAEQLYEALP
                     KATRDALGELPDVLERLQRDAQALRGKLEGLQDALTDAGGRAVTEGYADVRAARDAVQ
                     QRLADAVGALETIRLNLLRLHAGALTVEGLTTHLNVAAEVSDVVERLVAAQDEIESDL
                     RLPRARTPTPA"
     gene            3978..4568
                     /locus_tag="VFU45_05625"
     CDS             3978..4568
                     /locus_tag="VFU45_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5358573.1"
                     /translation="MPKGQKTRGRIVAQALKDASVVGLEGLSLGEVAEKVKMSKSGLF
                     AHFGSKEELQLQVLAHAAERFREVVILPAFKEPRGVPRLSALFERWLAWGSDPSIPGG
                     CVFTHAAVELDDQPGRVRDQLAEWQRQWRDTLAKAAALAVEERHFRPDLDTHQFAFQM
                     FGIVLAYYHAGRLLSDPDAGAQAHAAFDSLVAWASP"
     gene            4585..5490
                     /locus_tag="VFU45_05630"
     CDS             4585..5490
                     /locus_tag="VFU45_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5358574.1"
                     /translation="MPQSPAPANGRALGTAPWYIRSWFRAASAVAPSLAERQAASLFI
                     TPRRRKSIAPTDLHETLLPDRHAERRQGEAEPSFPKLSTGRIAAWSWGRGPTVLLTHG
                     WSGSAGDMAPLAAAFVQAGYRAVMIDMPGHGRSDPAATNLIVYLRTLAEMAARIGPLD
                     AVVGHSLGGTATALALGEGLIAARRAVLLAPGLSPWAFARDFAKVIGLPEARVPGMVR
                     RTEELVGRKADTLDVAATVTGLATPGLIAHAPEDADVPYAHSEAIAAAWRSARLVARP
                     GLGHRKILRDPETIAQAVEFVGRTP"
     gene            complement(5742..>6006)
                     /locus_tag="VFU45_05635"
     CDS             complement(5742..>6006)
                     /locus_tag="VFU45_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5358575.1"
                     /translation="LSLLALGAFGTAIAHVVMTAAAGRLGAARASSATFLMPPVALLL
                     GVLIRHESVSVVSIVGGAICVAGAWLMSHPRAVAVPALSKEAA"
BASE COUNT          911 a         2138 c         2128 g          829 t
ORIGIN      
        1 ggtacgccta cggaccgcca ccggtccggc tcctcctcga actccacctg acctacgccg
       61 acggccgcac cgaggtggtg acgagcgaca gcagctggcg gaccgcgccg agcccggtgc
      121 tgatctccga gatctacgac ggggagacgt acgacgcgcg gctggagcag cccgggtggg
      181 accggcccgg cttcgacgcg gcgtcgtggc ggcacgccat cgagctgccg ccggtcaagg
      241 tgaccatcca gccccaggcg atgccgccga tcgaggagac cgactccgtc cggccgatcc
      301 ggcggtggtc gcccggggcg ggcgtgtacg tcttcgacct ggggcagaac atcgccgggt
      361 gggcgcggct ccacgtgcag gggaaggcgg ggacgcgggt gcagatgcgc ttcgccgaga
      421 tcctcgaccc cgactcgagc ggcaacatca cccagatcaa cctgcggagc gccaaggcca
      481 ccgacgtcta cgtcctgcgc ggccgcggcg cggagacgtt cacgccgcac ttcacctatc
      541 acggcttccg ctacatcgag gtgaccggct atcccggggt gccgccgatg gacgcggtca
      601 tcggggtcgt ggcgcatacg gcgctccccg agaccggcac gttcgcctcg tcggacccga
      661 tgctcgatcg gctctggcag aacgtgatgt ggacccagcg cgccaacctc tacagcgtgc
      721 cgaccgactg cccccagcgc gacgaacgga tggggtggat gggcgacgcg caggtcttct
      781 ggcggacggc gagctacaac atggatatgg cggcgttcgg cgagaaatgg ctcgccgacg
      841 tgcgcgacgg acagacggca ccggggtgct tccccaactt tgcgccgaac ttcctgggcg
      901 tcctgacctg cggtgcgccg ggctgggccg acgcgggcgt catcatcccg tggaccaact
      961 ggcgccagta cggcgacacc gccgagctgg ccgcgcactg ggaggcgatg gagcgctaca
     1021 tggcgttcat cgccgactcg aacgccggct acctctggac ccggcggaag accgtcgagt
     1081 tcggcgactg gctccccgcc ggcagcacca cctcgtgggg acagaccaac accgacctca
     1141 tctcgaccgc gttctgggcg tacgatgcgt cactgatggc gcagatggcg cgcgccatcg
     1201 gccgagggga gcgggcggcg cattacgatt cgctggccgg ccggatccgc accgcattcg
     1261 accgcgcgtt cgtcctggcg gatgggcggg tcggcgcgcc cttcaccaac gccgacagca
     1321 tccactccga ctacacccag actgcgtcgg tgctggcgct ccgcttcggg ctcgttcccg
     1381 atagccagag ggccgcggtg gcgcggcacc tggtgctgga catccgggcc cacggcggcc
     1441 acctgaccac cggattcctc ggcagctcgt tcatcctccc cgtgctctcc gacgcgggcc
     1501 aggacgacgc ggccttcgag ctcctcttcc agcagagctt cccgtcgtgg ctctacgagg
     1561 tgaaggcggg tgcgaccacg acgtgggagc ggtggaacgg cgaccacggg aaccccgaga
     1621 tgaattcgta cagccactac gccttcggcg cggtgggcga atggctctac cggtacctgg
     1681 cggggatcga cgaggcgccc gacgcggtgg cattcgaccg gatcgtgatc catccgcgct
     1741 ggacgccgca gctcgattcc gtccgcgcca tctatcgctc ggcccgcggg gtcatcacca
     1801 gcgggtgggt gcgccagcgc gacgggtcga tcgtggtgag cggtcggatc ccggccaaca
     1861 cgaccggagc agtgatgctc cccgggtgga cggcaccgaa ggagatcgag gcgggggcgt
     1921 tccggttcgt cgtgcggggc tgagttcgtg gccgggggac gtcggcgtca ggccggcgtg
     1981 ggcgtccgcg cgcggggcag gcgcaggtcg gattcgatct cgtcctgggc ggcgacgagg
     2041 cgttccacca cgtcagacac ctcggcggcg acgttgagat gagtggtgag cccctcgacg
     2101 gtgagggcgc ccgcgtgcag gcgcaggagg ttgagccgga tcgtctcgag cgcgccgacg
     2161 gcatcggcca ggcgctgttg caccgcgtcg cgcgccgccc gcacgtcagc atatccctcc
     2221 gtgaccgcgc ggccaccggc gtcggtgagc gcgtcctgaa gcccctcgag cttcccccga
     2281 agcgcctgcg catcccgctg caatcgttcg agcacgtccg gaagctcgcc gagggcgtcc
     2341 cgggtggcct tgggcagggc ttcgtacagc tgctccgccg ccatcccgag cgagagctcg
     2401 gtggcccggt gggtcatcgc cggcccgatg gcgcgtttcc ccacgagcgg ccgggccagc
     2461 gagaaggcga gcctcccgat ccgtcccgcc cagaccttgg cccacagttc agcatcgatg
     2521 tcggtgcgcc gctggcggag cgccagcacg ccgagggccg ccgggcccgc caccatgccg
     2581 gagacgctga gcgcgcggac gatccacgag atcacggcgt cggaggtgac accaaggccc
     2641 ccgagggcca tcaggctgcc gaacgaaaga cccaccgata tccacgacca cttggcgatg
     2701 gggccgagca cccgctcggc ccggccggga acggcgcccc gtcctccccg ctcttcgcgc
     2761 cccgtttcga tctcgcgctt gaaggccgga atcacgtcgg cgtgagagta gccctgcatc
     2821 cggagcttgc gggcggcgtt gacgaggtac aggagcggcg gcacggtcgc accggccagg
     2881 aatgtggcaa gcgcccagcc cggaccgagg aagatcgatg cggcgccgat ggccgcgtag
     2941 agtgcgacga acgacaggat ggtcccgccg ccgtcgatcc gcccgcttcg cttggcaaag
     3001 acccggagcg cgacgggcag ctcccgccgc tgctccagcg ccgcgctcag ttgttcggcg
     3061 acggcctcgg cgctggccgg gcggtcagcg ggatcctttt cgagacatcg ctcgatgagc
     3121 tgggccacct tgcggggcac ggtgagtccg agggacagca gggacggcgc cggctcggtg
     3181 acctgcttcg ccagcacctc ggtcgcactg gcaccctcga acggcgggcg tccagcgaag
     3241 gcgaagaacg ccgtggcgcc gagaccgtac aggtcgctgc gggcgtcgat ggtgccggcc
     3301 aacgcctgtt ccgggctcat gaagtcgggg gtgccggaga tcccggcttc gaccgcatca
     3361 ccggctgccg cggcgatccc gaaatccacc accaacgccc ggcccgttgc cgcctcgagc
     3421 aggatgttgt caggcttgac gtcccggtgc acgagaccct gaccgtgcgc gtgcgcgagc
     3481 gcccacgccg cttcccggag catgcgcgtc ccatcggcga tcggaagcgg cccgcgctcg
     3541 aggacccgct cggtcagggt ctgaccatcg atgtaggcca tcacgaagaa gacgaaaccc
     3601 tccgtctccg cgacggcgtg gatcggcgtg atgttcgggt gcgagagctt ggcggcaagc
     3661 ctggcttcgc ggaggaagcg cgcgcggagc gccgggacgg cggcacgctc gggaggcagg
     3721 agcttgatgg ccaccaggcg gtcgagatgg acctcccggg ccaggtagac cacgcccatc
     3781 ccgcctcgac ccaattcccg gtcgatcgaa tagcggccgg ccagcgccga ctggaacgcg
     3841 aggaagagcg gatcgaccgg cggcgcggtg cgcatcccag aaggtatgga gcggtccatc
     3901 tcccggtaag ctgcgagcga cggggccgcc actggcttga gattgattat tagcacgacc
     3961 gttcgtatta ttgtgccatg cccaaaggcc agaagacccg cggtcggatt gtcgcccagg
     4021 cgctcaagga tgccagcgtg gtgggcctgg aggggctctc gctcggcgaa gtggccgaga
     4081 aggtcaagat gtccaagagc gggctctttg cccatttcgg gtcaaaggaa gagctccagc
     4141 tccaggtcct ggcccacgcg gcggagcgct tccgcgaggt ggtgatcctc cccgccttca
     4201 aggagccccg cggagttcca cggctctccg cgctcttcga gcgctggctc gcgtggggga
     4261 gcgacccgtc gatccccggc ggctgcgtct ttacccacgc cgccgtcgag ctcgatgacc
     4321 agcccggccg ggtccgcgac cagctggccg agtggcagcg gcagtggcgg gacacgctgg
     4381 ccaaggcggc cgcacttgcc gtggaggagc ggcacttccg acccgacctc gacacccacc
     4441 agttcgcctt ccagatgttc ggcatcgtcc tcgcgtacta ccacgccggc cggctcctgt
     4501 ccgaccccga cgccggcgct caggcccacg ccgcattcga ctcgctggtc gcgtgggcct
     4561 ccccctgacc cgacggcgcc cctgatgcca cagtcccctg cccccgcgaa cggtcgcgca
     4621 ctcggcaccg ccccctggta catccgctcc tggttccggg cggcgtctgc cgtcgcgcca
     4681 tcactcgccg agcggcaggc ggcgtcgctc ttcatcacgc cgcgccggcg gaagtccatc
     4741 gcaccgaccg acctgcacga gacgctcctc cccgatcggc acgcggaacg ccgccagggc
     4801 gaggccgagc cgtcgttccc caagctttcg acggggcgga tcgcggcgtg gtcgtggggg
     4861 cgcggcccga cggtgctgct gacccacgga tggtccggct ccgcgggaga catggcgccg
     4921 ctcgccgccg catttgtgca ggcgggctat cgcgccgtga tgatcgacat gccggggcac
     4981 ggccgctccg accccgccgc cacgaacctg atcgtctacc tccgcacctt ggcggagatg
     5041 gcagcgcgca tcggcccgct cgacgcggtg gtcggccatt cgctcggcgg caccgccacg
     5101 gcactggcgc tcggcgaagg gctcatcgcg gcgcgccggg cggtgctcct ggcgccggga
     5161 ctctcaccgt gggcattcgc gcgcgatttt gcgaaggtga tcgggctccc cgaggcgcgg
     5221 gtgccgggga tggtgcgccg caccgaggaa ctggtgggac ggaaggcgga taccctagac
     5281 gtggcggcca cggtgacggg gctcgcgacg ccggggctca tcgcccacgc gcccgaggac
     5341 gccgacgtcc cctacgcgca cagcgaggcg atcgccgcag catggcgcag cgcgcgactc
     5401 gtggcgcgcc cggggctcgg acatcggaag atcctgcgcg accccgagac gatcgcacag
     5461 gcggtggaat tcgtgggccg taccccctga cccggggtga tgtgggggtc gcctgcggcg
     5521 acccgaccct tggccaccat cgcacccgga cgtgcaggcg gtggcctgcg gtcacctcga
     5581 ccagccggat gccagcacca cggccgtagg ggcgcagcac gctgcaccct tacaggccgc
     5641 ccgcgatcaa caagatgcgg cggcgcgaac gcgcatcctc accccctgcc cccctctcca
     5701 tcctgacgga cggagagggg gaaaggcatt cgggctccgc cctacgccgc ctctttcgac
     5761 agcgccggga ccgcgacggc acgcgggtgg ctcatcagcc aggcaccagc gacacagatg
     5821 gcgccaccga cgatcgacac cacgctgacg ctctcgtgcc ggatgaggac gccgagcagg
     5881 agggccaccg gcggcatcag aaaggtggcg gatgacgcgc gggcggcgcc gaggcggccg
     5941 gccgcggcgg tcatcaccac gtgggcgatg gcggtgccga acgcgcccag ggccaggagc
     6001 gagagg
//
LOCUS       DASUAP010000218         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_101564, whole genome shotgun sequence.
ACCESSION   DASUAP010000218 DASUAP010000000
VERSION     DASUAP010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101564"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..583)
                     /locus_tag="VFU45_05640"
     CDS             complement(179..583)
                     /locus_tag="VFU45_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU5358576.1"
                     /translation="MPSTRRFIPTKEWEDERQLRGLDGEMAALAYLTACGWSIEAHRF
                     KLGRHDIDLIARRGGIVAFIEVKTRRGSAFGTPVESVDRRKRQAVAKVAALWLLRFGR
                     PNDTYRFDVIAVHEGKGGARRIEHVEDAWRGG"
     gene            complement(725..1750)
                     /locus_tag="VFU45_05645"
     CDS             complement(725..1750)
                     /locus_tag="VFU45_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5358577.1"
                     /translation="MSDPSDVDAPAGGLDRRTFLTVATAVPLAAAALAACEGARPAQG
                     GGAAAVAAADTSTAGLPVFRRFDPELPPLAPGRVRDFHLEARELDLRISDELVAHAWS
                     FDGSVPGPVFRARVGDTVNITLTNTGNTPHSVDFHAGQVDPKRAFKTIAKGQTAQYSF
                     TPKYAGAFLYHCGTQPVLMHMGMGMYGIIIVDPATPLPKAREFVLLQSEFYLAPPAGK
                     PIRTFDYQLMLGTLPAVVAFNGYANQYLKEPIRVRRGERVRLYVGNAGPTLGCSFHIV
                     GEQFDTVYLGAPPGNAIHGVQTYNVPAGGGMIFELTADVPGEFPFVNHAFGHGQKGAM
                     GMLIVEE"
     gene            complement(1853..>3902)
                     /locus_tag="VFU45_05650"
     CDS             complement(1853..>3902)
                     /locus_tag="VFU45_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU5358578.1"
                     /translation="LDMKRTALRVGGLAVLIPLFIGTLLDLGPALFDPPLHEWVLSAR
                     VVGLIPGFFAHGLTALLGRPIAALIAFGLTSALTILTVGWHPLQRLERRAQQEPAFQP
                     VARVPEPRPLPVAGDEGGEGEGGGEEGHAALRTVAKKKPARKPLPKPKPVVPGEPLDP
                     NDRWTTELLHEAPASSGEAAKRGQLEELKARLQEALTTFKVPSTIADYTSGPVVTQFY
                     ARLEAGVKLNRLVQLADDLALAMKARSIRVARVPGKDVVGVEVPNPKAEMVVVRQILE
                     SPEWDAEGWTLPIALGLDLEGRAIVADLAKMPHLLIAGATGTGKSVAINAIITSLIYH
                     YQRREDLRLLMVDPKMVELSMYNSLPHLRHKVVTNNNDAATVLKWAVNEMERRYVLLQ
                     TNGARNVADFNRKVEEGKPLKPIPRPKATLSSISAEAESTAPEAPEPDTYTEGKLPLI
                     VILVDELADLMMTVQGEVETPLARLAQKARAIGIHLILATQRPSVNVITGLIKANFPS
                     RIAFRVASKVDSRTILDQNGAEALLGNGDMLFLPPGRNEPMRIQGAYISTEETERIMD
                     WYTHRKDALDAAAAAGPADYDIIAETRKLEAEAEGGAPGEGGDDERDPLFREAAEACI
                     QNQGGSTSLLQRKLRIGYGRAARIIDQLHYAGILGPPDGSKPREVLVGYDQLDEYAGG
                     "
BASE COUNT          525 a         1397 c         1351 g          629 t
ORIGIN      
        1 gcgtgggggt cgcctgcggc gacccgacgc ccagcaaccg cccgtcatcc tggggggcct
       61 tcaggccccg aaggatctcg acccgaaact cgtctccgcg tcggaatgcc gcccctcgga
      121 ccggtgtcgg gtcgccgcag gcgaccccca cgggatcatt ccatccgccc gccacccatc
      181 accctccccg ccacgcatcc tccacgtgct cgatccggcg ggcgccgccc ttgccttcgt
      241 gcaccgcgat cacgtcgaac cggtaggtgt cgttggggcg cccgaagcgg aggagccaga
      301 gcgccgccac cttggccacc gcctggcgct tgcggcggtc caccgactcg accggcgtcc
      361 cgaacgcgct gccgcgccgg gtcttgacct cgatgaacgc cacgatcccg ccccgccggg
      421 cgatcaggtc gatgtcgtgg cggccgagct tgaagcggtg ggcctcgatg gaccagccgc
      481 aggcggtgag gtaggcgagt gccgccatct ccccgtcgag gccgcggagc tggcgttcgt
      541 cctcccactc cttggtgggg atgaaccgtc gggtgcttgg catggcgcca cgataacgga
      601 aggcgggggg agctccgtca tccggacgac gcggcgatgg acctgggaac gcaacgacga
      661 acggggcccc tggtcagcga ggccccgtcc gatcacccga cacttcgacc accctgccac
      721 accgctattc ctccacgatc agcatcccca tggcgccctt ctgtccgtgg ccgaaggcgt
      781 ggttgacgaa ggggaactct cccggtacgt cggcggtgag ctcgaagatc atcccgccgc
      841 cggcggggac gttgtacgtc tgcaccccgt ggatcgcgtt ccccggcggc gccccgaggt
      901 agacggtgtc gaactgctcc cccacgatgt ggaacgaaca gccgagggtg gggcccgcgt
      961 tcccgacgta gaggcggacc cgctcgccgc gccggacccg gatcggctcc ttcaggtact
     1021 ggttggcgta tccattgaag gcgaccaccg ccggaagggt gcccagcatc aactggtagt
     1081 cgaaggtgcg gatcggcttc ccggccggcg gggcgaggta gaactcgctc tggagcagga
     1141 cgaactcccg ggccttcggg aggggggtcg ccggatcgac gatgatgatg ccgtacatcc
     1201 ccatccccat gtgcatcagc accggctggg tcccgcagtg atagaggaac gcgcccgcat
     1261 acttgggggt gaagctgtac tgcgccgtct ggcccttcgc gatcgtcttg aacgcccgct
     1321 tggggtcgac ctggccggcg tggaagtcca ccgagtgggg ggtattgccg gtgttggtga
     1381 gggtgatgtt gacggtgtcc ccgacccgcg cgcggaagac cggccccggc acgctgccgt
     1441 cgaagctcca ggcgtgggcc accaactcgt cggagatccg gaggtcgagc tcccgggcct
     1501 cgaggtggaa gtcacggacc cgtccaggcg cgagcggggg cagctcggga tcgaatcgcc
     1561 ggaagaccgg gagtccggcg gtcgaggtgt ccgccgcagc gaccgccgcc gcaccgcccc
     1621 cctgcgcggg ccgcgccccc tcgcacgccg cgagcgccgc ggccgcgagc gggaccgccg
     1681 tggcgacggt caggaaggtc cggcggtcga ggccgccggc gggtgcatcg acatccgacg
     1741 gatcgctcat gggtcactcc gttctcgcag gtagtcctct catacgcccc aagcgttgct
     1801 gcatccggct tgtcatgcgt tcgtccaccc atccgcccat gcgtccatcc gcctaccccc
     1861 ccgcgtactc atccagctga tcatacccca ccagcacctc gcgcggcttg ctcccgtcgg
     1921 gagggccgag gatccccgca tagtgcagct ggtcgatgat ccgggccgcg cggccatacc
     1981 cgatgcgcag tttccgctgc aggagcgacg tgctcccccc ctggttctgg atgcacgcct
     2041 ccgccgcctc gcggaagagc gggtcgcgct cgtcgtcccc gccctccccc ggcgcgcccc
     2101 cctccgcctc cgcctcgagc ttccgcgtct ccgcgatgat gtcgtagtcc gccgggcccg
     2161 ccgcggccgc cgcgtcgagg gcatccttcc ggtgggtgta ccagtccatg atccgctcgg
     2221 tctcctcggt cgagatgtac gcgccctgga tccgcatcgg ctcgttccgc cccggcggga
     2281 ggaagagcat gtcgccgttg ccgaggagcg cttccgcacc gttctggtcg aggatcgtcc
     2341 ggctgtccac cttggacgcc acccggaagg cgatccggct cgggaagttg gccttgatga
     2401 ggccggtgat gacgttcacc gacggccgct gggtggcgag gatcaggtgg atgccgatgg
     2461 cgcgcgcctt ctgcgccagc cgggcaaggg gtgtttccac ctccccctgc acggtcatca
     2521 tcaggtcggc cagctcgtcg accaggatca cgatgagcgg gagcttcccc tcggtgtacg
     2581 tgtccggctc cggcgcctcc ggggcggtgc tctccgcctc cgcgctgatg ctgctcagcg
     2641 tcgccttggg ccgggggatc ggcttgagcg gcttcccctc ctcgaccttc cggttgaagt
     2701 cggcgacgtt gcgggccccg ttggtctgga gcagcacgta gcgccgttcc atctcgttga
     2761 cggcccactt gagcacggtg gcggcgtcgt tgttgttggt gacgaccttg tgccgcaggt
     2821 gggggagcga gttgtacatc gacagctcga ccatcttcgg gtccaccatc aggagccgga
     2881 ggtcctcccg ccgctggtag tggtagatga ggctcgtgat gatcgcgttg atcgccaccg
     2941 acttgccggt gccggtggcg cccgcgatga gcaggtgggg catcttggcc aggtcggcca
     3001 cgatggcccg gccctcgagg tcgaggccca gcgcgatcgg gagcgtccac ccttcggcgt
     3061 cccactcggg gctctcgagg atctggcgca ccaccaccat ctcggccttc gggttcggca
     3121 cctcgacccc gaccacgtcc ttccccggca cccgcgccac ccggatggac cgcgccttca
     3181 tcgcgagcgc caggtcgtcg gccagctgga ccagccggtt gagcttgacc cccgcctcga
     3241 gccgggcgta gaactgggtc accaccggcc ccgaggtgta gtcggcgatc gtcgagggca
     3301 ccttgaaggt ggtgagcgcc tcctgcagcc gggccttcag ttcctcgagc tgtccccgct
     3361 tggccgcctc cccgctcgac gccggcgcct cgtgcagcag ctcggtggtc cagcggtcgt
     3421 tggggtcgag cggctccccc ggcaccaccg gcttgggctt ggggagcggc ttccgggccg
     3481 gcttcttctt cgccacggtc cggagagcag cgtgcccctc ctctcctcct ccttctcctt
     3541 ctcctccttc gtcccccgcg accggcagcg gccggggttc gggcacgcgc gcgaccggct
     3601 ggaacgcggg ctcctgctgg gcgcggcgct ccaggcgctg gagcgggtgc cagccgacgg
     3661 tcaggatggt gagggcggag gtgagcccga aggcgatgag ggccgcgatc gggcgcccga
     3721 ggagtgcggt gagcccgtgg gcgaagaacc cggggatgag ccccaccacc cgggcggaga
     3781 gcacccactc gtggagtggc ggatcgaaga gcgcggggcc caggtcgagc agggtgccga
     3841 tgaagagcgg gatgagcacg gcgagcccgc ccacccggag ggcggtgcgc ttcatgtcga
     3901 gc
//
LOCUS       DASUAP010000219         6346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_728489, whole genome shotgun sequence.
ACCESSION   DASUAP010000219 DASUAP010000000
VERSION     DASUAP010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728489"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFU45_05655"
     CDS             <1..939
                     /locus_tag="VFU45_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HEU5358579.1"
                     /translation="VVLANPRLFRPFEALIGLMPLPRYGSIDPTPFVAVFFPAYFGLM
                     VGDIGYGIVLAILGLLLHRRSRPGTMLRNVAEIIGPCALAAIVGGVLYGEFLGDLGTR
                     WFGLRPLLFSREEAIVPFLLLSVAVGAVHILLGLVLGAVNARREPRRAAGQGVSALMV
                     VLVILALLAAAEVLPRGFFSPAVVGLLIAFPVLIALEGVVAMIELLTTFGNILSYARI
                     MALGVASVMLAVVANRMVGALGSVLVGVLFALLFHAVNFAIAMFSPTVHALRLHYVEF
                     FGKFYSPGGVRYQPLGHWSRAPGPARPTPAPMRHSA"
     gene            936..1157
                     /locus_tag="VFU45_05660"
     CDS             936..1157
                     /locus_tag="VFU45_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358580.1"
                     /translation="MTPAAFTVLAAALAVAVPALATAWAQSRIGPAIAASMAEKPELS
                     TTAVLMIAIPETMVVLGFVVSMVILVRAG"
     gene            1170..1757
                     /locus_tag="VFU45_05665"
     CDS             1170..1757
                     /locus_tag="VFU45_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358581.1"
                     /translation="MALDQLLAALERDAHAAADRALADARREAARIEADTERDIAVRR
                     ESAAGAVIAGQRAALEQELAGAGRRVRAEVLGARERLLARVSAAMRAALPAAAAGPAY
                     RASLPGRIAAARGYLGPDEPVVLECPPDLARLAAPLVTADRTVSIRTVEGRGSGFRLA
                     TADGVVEVDDTLESRLASSWRLLARTALERLGLSR"
     gene            1754..2779
                     /locus_tag="VFU45_05670"
     CDS             1754..2779
                     /locus_tag="VFU45_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEU5358582.1"
                     /translation="MRPAWDDVNARARGLSTHLLTRGTLHALGRAPELAALASGLRRC
                     GVLAATAEEQPTPEQLELGIRRWAGARLGLLARWVGERSAALPLVFDAEDRRSLRAIF
                     RGIAERAPAERRLAGLIPTPALPERALEALAAAPTAAAAAALLAAWRHPLAAAAAWTG
                     AADPDLFALDESLARAGADQARRAARRTHDAPLRNAVREAIDLDNALTAVVLATAGRE
                     VPSASQFLPGGARVTSVVFREAIATGDPGAAGVRLARALAGSAYADLFARGAGDPAAL
                     ESAVLRRQLRELSRQVRRAPLGPLELIWFALRLRAQVLDLQRIIWTVALDAPRAELAG
                     ALVTAEP"
     gene            2776..3105
                     /locus_tag="VFU45_05675"
     CDS             2776..3105
                     /locus_tag="VFU45_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEU5358583.1"
                     /translation="MSVGVRIVCRPVLAPGFGLTSLRVSEAASAADADVTVRGLVGDP
                     AVGILLVEDHVHDRLPDELRRRLARRPTPILVPFPGPAWEERRDAAESYIIELLRQII
                     GYRVRLR"
     gene            3102..4835
                     /locus_tag="VFU45_05680"
     CDS             3102..4835
                     /locus_tag="VFU45_05680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEU5358584.1"
                     /translation="MTAPVLVRVSGALAEARPLRGATLYELARVGDRGLFAEVVRLDG
                     DRGTLQVYEETNGLAIGEPVALTGGPLTVELGPGLLGSVLDGVGRPLRRIAETSGDLI
                     AAGTSIATLEPGRKWSFTPVAAVGASVTGGDVLGTVEERPGFVHRVLVPPGVTGTIAA
                     LEAGSAGVWDPVGRLADGTPLTLAHRWPVRRPRPVAGRLPLRRPFVTGQRVFDFLFPV
                     AEGGSVAVPGGFGTGKTVIEQSLAKYAEADVIVYVGCGERGNEMAEVLDEFPRLVDQR
                     TGRSVMDRTVLLVNTSNMPVAAREASVYVGITVAEYYRDMGYRVALMADSLSRWAEAL
                     REIGARLQEMPGEEGYPTYLGNRLGKFYERAGHVRALGAPEREGALTLISAISPPGGD
                     FSEPVTQASLRVAGALWALDPALAHQRQFPAVDWETSYSLYADDLAPWLRQHGGADWP
                     DLRRATLELLQRDRELREVAALVGPESLQDADRLLLECARMIRDTVLGQSAYDANDAY
                     SPIGKTWLLAALAHEVLARAQGALAAGRAFEQLDLATVERLLSALRLAPVEELEARSR
                     AVRAALEDSAR"
     gene            4832..6217
                     /locus_tag="VFU45_05685"
     CDS             4832..6217
                     /locus_tag="VFU45_05685"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEU5358585.1"
                     /translation="MSAPPRRHHGAEGVGGPLLYLKRTHRVALGEWVDIRVPGQRPWR
                     GQVIDAGREVSVIQLLEDTVGLAPATVELTLTGEGATTVVGRELLGRAFSGTGAPIDG
                     LPAPVGDATVPIWGAPLNPVRRARPADFIETGISAIDGMNTLVRGQKLPVFSGPGLPG
                     LDLAARIVEGARAPRDEPFAVVFAGIGIAAREADAFLERFDASGARERSVLYLNETSD
                     PSIERLLAPRVALAQAEHLAFELGMQVLVVIADLVHYCEALREIATARQEIPGRRGYP
                     GYMYSDLASIYERAGVLAGKPGSVTQIPILTMPDDDITHPIPDLTGYITEGQVVLSRD
                     LHRRGVSPPVDVLPSLSRLMQSGIGAGRTVPEHREWSNQLYATYTRGRDARLMAAIVG
                     EAGLAAVDRRALAFADRFELEFVGQGGARRPIADTLAVGWRLLATLPREDLHRVTDAT
                     WASHVPSGAPR"
BASE COUNT          707 a         2316 c         2386 g          937 t
ORIGIN      
        1 gtggtgctcg ccaatccacg gctcttccgc ccgttcgagg cgctgatcgg cctgatgccg
       61 ctcccgcgtt acggctccat cgacccgacc ccgttcgtgg cggtcttctt cccggcgtac
      121 ttcggcctca tggtgggcga catcggctac ggcatcgtgc tcgccatcct cgggctcctg
      181 ctgcaccgcc gctcccggcc cggcacgatg ctccggaacg tcgccgagat catcgggccg
      241 tgcgccctgg ccgcgatcgt gggcggcgtc ctctatgggg agttcttggg cgaccttggc
      301 acccgttggt tcggccttcg gccgctcctg ttcagccggg aagaggcgat cgtgcccttc
      361 ctcctcctga gtgtggccgt cggcgccgtg cacatcctcc tcggcctggt cctcggtgcg
      421 gtgaacgccc ggcgcgagcc ccggcgggcg gcgggccagg gagtgtccgc cttgatggtg
      481 gtgctggtca tcctggcgct gctcgccgcg gcggaggtgc tcccccgcgg gttcttctcg
      541 ccggccgtcg tcgggctgct catcgcgttc ccggtgctga tcgcgctgga aggggtggtc
      601 gcgatgatcg agctgctcac cacgttcggc aatatcctct cctacgcccg catcatggcg
      661 ctcggtgtcg cgtcggtgat gctggcggtg gtggccaacc ggatggtcgg cgccctcggc
      721 agcgtcctgg tcggcgtgct cttcgcgctg ctgttccacg cggtcaattt cgccatcgcg
      781 atgttcagcc cgacggtcca cgcactgcgg ctccactacg tcgagttctt cggcaagttc
      841 tacagcccgg gcggggtgcg ataccagccg ctcggccact ggtcccgagc gccgggcccg
      901 gcccgtccca cccctgctcc catgaggcac tccgcatgac gcccgctgcc ttcaccgtcc
      961 tcgccgcggc cctcgccgtg gccgtcccgg ccctcgcgac cgcctgggcc cagtcgcgca
     1021 tcggcccggc catcgcggcg tcgatggccg agaagcccga actctcgacc accgcagtgc
     1081 tgatgatcgc gatccccgag acgatggtgg tgctcggctt cgtggtctcg atggtgatcc
     1141 tggtccgggc cggctgaggg cggcgcgccg tggccctcga ccagctcctc gcagcgctcg
     1201 agcgcgacgc gcacgccgcc gccgaccggg cacttgccga cgcccggcgc gaggcggcgc
     1261 ggatcgaggc cgacaccgaa cgcgacatcg ccgtgcgccg ggaatccgcc gccggtgccg
     1321 tgatcgccgg tcagcgcgcc gcgctcgaac aggagctggc cggcgcgggc cggcgggtcc
     1381 gggcggaggt gctcggggcg cgcgaacgcc tgctggcccg cgtgtccgcc gcgatgcgcg
     1441 cggcgctgcc ggcggctgcc gcggggcccg cgtatcgggc gtcgctcccc ggccgcatcg
     1501 cggccgcccg cggatacctc ggtccggacg aaccggtggt gctcgagtgt ccgccggact
     1561 tggcccggct cgctgccccg ctcgtgacgg cggaccgcac cgtctccatc cggacggtcg
     1621 agggccgggg cagtgggttt cggctggcga cggcggacgg cgtggtcgag gtcgacgaca
     1681 ccctcgagtc ccgtctggcg tcgtcctggc gactgctcgc gcggaccgcg ctcgagcggc
     1741 tgggcctctc ccgatgaggc ccgcctggga cgatgtcaac gcccgcgcgc ggggactctc
     1801 cacgcacctg ctcacgcggg gaacgctgca tgcgcttggc cgcgcgccgg agctcgccgc
     1861 gctcgcgtcg ggcctgcggc ggtgcggcgt gctggccgcg actgcggagg aacagcccac
     1921 acccgagcag ctcgagctcg gcatccgccg gtgggccggt gcgcggctgg ggttgctcgc
     1981 gcggtgggta ggcgagcggt cggcggcgct cccgctcgtc ttcgacgcgg aggatcgccg
     2041 gagcctgcgc gccatcttcc gtgggatcgc ggagcgggcc cccgccgagc ggcggctggc
     2101 cggcctgatc cccacgccgg ccttgccgga gcgcgcactc gaggcgctgg ccgcggcgcc
     2161 caccgccgcc gccgcggcgg cgctgctcgc ggcgtggcgt cacccgctgg cggccgcggc
     2221 ggcctggacc ggtgcggccg atccggacct gttcgccctg gacgaatcgc tggcacgcgc
     2281 cggggccgac caggcccggc gcgcggcgcg ccggacgcat gacgcccccc ttcggaatgc
     2341 ggtccgggag gcgatcgacc tcgacaacgc cctgaccgcg gtggtgctcg ccaccgccgg
     2401 gcgcgaggtg ccgtcggcgt cgcagttcct tccggggggc gcgcgggtga cgtcggtcgt
     2461 ctttcgcgag gcgatcgcga ccggcgatcc gggcgccgcc ggcgtacgcc tggcgcgggc
     2521 gctggccggc tcggcgtatg ccgacctctt cgcgcgcggt gccggggatc cggcggcgct
     2581 cgagagtgcg gtccttcgcc ggcagttgcg ggagctgtcc cggcaggtgc gccgcgcgcc
     2641 gctcggcccg ctggaactca tctggttcgc gctgcggctc cgcgcccagg tcctcgatct
     2701 gcagcggatc atctggacgg tggctctcga tgcgccgcgg gcggagctcg ccggggcgct
     2761 cgtcacggcg gagccatgag cgtcggcgtc cgcatcgtct gccgccccgt cctggcgccc
     2821 ggattcggcc tgacgagcct gcgggtatcc gaggcggcct cggcggcgga cgccgatgtg
     2881 acggtgcggg ggctcgtcgg cgatcccgcc gtcggcatcc tccttgtcga ggaccacgtg
     2941 cacgaccggc tgccggacga actccggcgg cggctggccc gccgaccgac cccgatcctg
     3001 gtgccgttcc ccggccccgc gtgggaagaa cggcgcgacg cggcggagtc atacatcatc
     3061 gagctgctcc gccagatcat cggctaccgg gtgcggctcc ggtgacggcc ccggtcctgg
     3121 tgcgggtgag cggcgcactg gcggaggccc ggccgctccg cggtgccacg ctctacgagc
     3181 tggcacgcgt gggcgatcgc ggcctcttcg cggaagtggt ccgcctggac ggcgaccggg
     3241 gaacgctcca ggtctacgag gagaccaacg ggctcgccat cggggagccc gtggcgctca
     3301 ccggcggccc gctcacggtc gagctcggtc cggggctcct cgggtcggtg ctcgatggcg
     3361 tcggacgccc gctccgacgc atcgccgaga cgagcggcga cctgatcgcc gcgggcacgt
     3421 cgatcgccac gctcgagccg ggccgcaaat ggagcttcac gccggtggcg gcggtcggcg
     3481 cgtcggtgac cggcggcgat gtcctcggca ccgtggaaga acgtcccgga ttcgtccatc
     3541 gggtgctggt gccacccggg gtcaccggga ccattgcggc actcgaggcg ggaagcgccg
     3601 gggtctggga tccggtgggg cgcctggccg acgggacccc gctcacgctg gcccatcggt
     3661 ggccggtgcg gcggccgcgg cccgtggccg gtcggttgcc gctccggaga ccgttcgtca
     3721 ccggccagcg ggtgttcgac ttcctctttc cggtggccga gggcgggagc gttgcagtcc
     3781 cgggcggatt cggcaccggc aagacggtga tcgaacagtc gctcgccaag tacgccgagg
     3841 ccgacgtgat cgtctacgtc ggctgtggcg agcggggcaa cgagatggcg gaggtgctcg
     3901 acgagttccc ccgcctggtg gaccagcgca ccgggcgctc ggtgatggac cgcaccgtgc
     3961 tcctggtgaa cacctcgaac atgccggtgg cggcgcgcga agcgtcggtg tacgtcggca
     4021 tcaccgtcgc ggagtactac cgggacatgg ggtaccgggt ggcgctgatg gccgacagcc
     4081 tctcgcgttg ggcggaggcg ctccgggaga tcggcgctcg cctgcaggag atgccggggg
     4141 aggaaggcta cccgacgtac ctcggcaacc ggctcggcaa gttctatgag cgtgccggcc
     4201 acgtgcgcgc gctgggggcc cccgagcggg agggtgcgct caccctgatc agcgccatct
     4261 cgccgcccgg aggcgacttc tcggagccgg tgacgcaggc gtccctccgg gtggcgggcg
     4321 cgctctgggc gctcgatccc gcgctggcgc accagcgcca gttcccggcg gtcgactggg
     4381 agacgagcta cagcctctac gccgacgacc tcgcgccgtg gctccggcag cacggcggcg
     4441 cggactggcc ggatctccgg cgtgcgacgc tcgaacttct gcagcgcgac cgggagctgc
     4501 gcgaggtcgc ggcgctggtg ggccccgaat cgctgcagga cgctgaccgg ctgctcctcg
     4561 agtgcgcccg gatgatccgc gacacggtgc tgggccagag cgcctacgac gccaacgacg
     4621 cctactcgcc catcggcaag acgtggctcc tcgcggccct ggcgcacgaa gtcctggcgc
     4681 gggcgcaggg cgcgctcgcg gcgggcaggg cgttcgagca gctcgacctc gccaccgtcg
     4741 agcggctcct cagcgccctg cgcctggcgc cggtcgagga gctggaagcg cgcagccggg
     4801 cggtgcgggc ggcgctcgag gactcggcgc ggtgagcgcg cccccgcgtc gccaccatgg
     4861 tgccgagggc gtcggcgggc cgctcctcta cctcaagcgc acccaccgcg tggcgctggg
     4921 ggagtgggtg gacatccgcg tgccggggca gcgcccgtgg cggggacagg tcatcgacgc
     4981 cggccgcgag gtgagcgtca tccagctgct ggaggacacc gtgggcctcg cgccggccac
     5041 ggtggagctc accctcaccg gggagggcgc gacgaccgtg gtcgggcggg agctcctcgg
     5101 gcgcgcgttc agcgggaccg gggcgccgat cgacggcctc cccgcgccgg tgggcgatgc
     5161 caccgtgccg atctggggtg caccgctcaa tccggtccgg cgcgcccggc ccgccgactt
     5221 catcgagacc ggcatctcgg ccatcgacgg catgaacacg ctggtccggg ggcagaagct
     5281 gccggtcttc tccggtccgg ggctgccagg cctcgatctc gcggcgcgga tcgtcgaggg
     5341 cgcgcgcgcg ccgcgcgacg aaccgttcgc ggtcgtgttc gcgggcatcg gtatcgcggc
     5401 acgcgaggcc gacgccttcc tcgagcggtt cgacgcgtcc ggtgcccggg aacggtcggt
     5461 gctgtacctc aacgaaacga gcgacccgtc gatcgagcgg ctcctggccc cccgcgtggc
     5521 gctggcgcag gcggagcatc tggcgttcga gctggggatg caggtgctcg tcgtgatcgc
     5581 cgacctggtc cactattgcg aggcgctccg cgagatcgcc accgcgcgcc aggagatccc
     5641 ggggcgccgc ggctatccgg ggtacatgta ctccgatctt gccagcatct acgagcgtgc
     5701 cggcgtgctg gccgggaagc cggggtcggt cacgcagatc ccgatcctga cgatgcccga
     5761 cgacgacatc acccacccga tcccggacct caccggctac atcaccgaag gacaggtggt
     5821 gctgtcccgc gacctccatc ggcgtggcgt ctcaccgccg gtggacgtcc tgccttcgct
     5881 ctcgcggctg atgcagtcgg ggatcggcgc cggccgcacc gtgcctgagc accgggagtg
     5941 gtcgaaccag ctgtacgcca cctacacccg tggccgggac gcccggctga tggccgccat
     6001 cgtcggcgag gcgggcctgg ccgcggtcga ccgccgggcg ctggcgttcg ccgaccggtt
     6061 cgagctggag ttcgtcggtc agggtggggc gcggcgcccg atcgccgata cgctcgcggt
     6121 gggctggcgg ctccttgcca ccctcccgcg cgaggacctg caccgggtca ccgacgccac
     6181 ctgggccagc cacgtgccgt cgggggcacc ccggtgagcg cccgatccgt gccggccacc
     6241 cggctcgagc ggctgcgctc ccgggtccgc ctggcgcggc tcgaccgtgg agcggaactg
     6301 ctgcgtcgga agcgcgaggc gctggtggcg gagctctttc gcctgg
//
LOCUS       DASUAP010000220         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_755413, whole genome shotgun sequence.
ACCESSION   DASUAP010000220 DASUAP010000000
VERSION     DASUAP010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755413"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFU45_05690"
     CDS             complement(<1..457)
                     /locus_tag="VFU45_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEU5358586.1"
                     /translation="MSTRVHPTALVDPDARLGADVEVGPFAIVEGAVEVGDRCRIGAR
                     ATLHRFTRLAHDVTIGDGTILANLPQDHKFKNEETWVEVGAHSTIREYSTINRGTTQS
                     YKTTVGERCYLMSYVHLGHDCHIGNGVTIANGTQLSGHVTVHDRANISGL"
     gene            complement(454..888)
                     /gene="fabZ"
                     /locus_tag="VFU45_05695"
     CDS             complement(454..888)
                     /gene="fabZ"
                     /locus_tag="VFU45_05695"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU5358587.1"
                     /translation="MDIGKILNVMPHRYPFLLVDRILEVEEGKRIVGLKNVTINEPFF
                     VGHFPGHPIMPGVLIIEAMAQVGGMLLLGFFPEEEHAQKVVYFMSLDNVKFRRPVLPG
                     DQLRCELDMLQFRGKTCRMKGVAYVDGNVVCEAEMMARVVDR"
     gene            complement(888..1727)
                     /gene="lpxC"
                     /locus_tag="VFU45_05700"
     CDS             complement(888..1727)
                     /gene="lpxC"
                     /locus_tag="VFU45_05700"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEU5358588.1"
                     /translation="MPRRTLARPAEFRGTALHTGVETRLRVGPAGTGTGIVFARTDLA
                     GVPRIPARLAEVEATERRTAIGRPPATIHTVEHLLAALQALEVDDALVELDGPEPPIL
                     DGSFAPFVEGLLDAGLADQAGTPAEWRVPAPLEVVVAGSRYRAEPSATLRVSVTVEWA
                     HPAIGRQSGSWELTPGVFRRELAGARTFGLVREVEALQARGLALGASYDNAIGLDDRG
                     VVGTVLRWPDEFVRHKAGDLVGDLALLGGRIRAHVLAERPSHHGNVMLARAIASAAKE
                     IDT"
     gene            complement(1720..2760)
                     /gene="lpxD"
                     /locus_tag="VFU45_05705"
     CDS             complement(1720..2760)
                     /gene="lpxD"
                     /locus_tag="VFU45_05705"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU5358589.1"
                     /translation="MASDRAPLTAEAVAELVGGRLVGAGGAAVTAIGPLDRAGPETLS
                     FLTGPRYVPAFRASRAGLVLLPEQYAAEAAGPACRIVVADPYRAVQAVAAVLYPTVAP
                     EPGIHPSAILGPGASIGAGASLAPYVVLGRDSRVGARVILGAHVILGDGVTVGDDTVI
                     DPGVTVYPGSIIGRRVVIKAGAVIGSPGFGFMEGAAVPDRRVHIGRCIIGDDVEVGAN
                     ATVDRGSLDDTVLGDGTKLDNMVHVGHNVRIGRRCLVAAQCGFAGSVRVGDGVLIGGH
                     TAVNGHRTVGDGARIAGFSAVWGDVPAGETWGGLPARPQQETLRRQAALGRLTGISAK
                     LERLVERARPDA"
     gene            complement(2761..>3076)
                     /locus_tag="VFU45_05710"
     CDS             complement(2761..>3076)
                     /locus_tag="VFU45_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU5358590.1"
                     /translation="AANEFQTSSVLLSPTARQQKQQGLVQQQQDIEQKTQELQTRMQS
                     RERELIEPIQARVQAVIEGIRAEGNYAMIFDVSAMGGAVVAADRSLDLTQTVIDRLQA
                     AK"
BASE COUNT          428 a         1160 c         1050 g          438 t
ORIGIN      
        1 ccaggccgct gatgttggcg cgatcgtgca ccgtcacatg ccccgagagc tgggtgccgt
       61 tggcgatcgt gacgccgttc ccgatgtggc agtcgtggcc caggtggacg tagctcatca
      121 ggtagcagcg ctcgcccacc gtggtcttgt agctctgggt ggtgccgcgg ttgatcgtgg
      181 aatactcgcg gatcgtcgaa tgggcgccga cctcgaccca cgtctcctcg ttcttgaact
      241 tgtggtcctg cggcaggttg gcgaggatgg tgccgtcgcc gatcgtcacg tcgtgggcca
      301 gccgggtgaa gcgatgcagc gtcgcccggg cgccgatacg gcaccggtcg ccgacctcga
      361 ccgcgccttc cacgatcgcg aacggcccga cctcgacgtc cgccccgagc cgggcgtcag
      421 gatccaccag cgccgtcgga tggacgcgcg tgctcaccgg tccaccacgc gggccatcat
      481 ctcggcctcg cacaccacgt tcccgtcgac gtacgccacg cccttcatgc ggcaggtctt
      541 cccgcggaac tgcagcatgt cgagttcgca acgcagctgg tcgcccggca gcacggggcg
      601 gcggaacttc acgttgtcga gcgacatgaa gtacaccacc ttctgcgcgt gctcctcctc
      661 cgggaagaac cccaggagga gcatcccgcc cacctgcgcc atcgcctcga tgatcagcac
      721 gcccggcatg atgggatgcc cgggaaaatg cccgacgaag aagggctcgt tgattgtcac
      781 gttcttcagc ccgacgatcc gcttcccctc ctcgacctcc aggatccggt ccaccaggag
      841 gaacgggtat cggtggggca tcacattgag gatcttgccg atgtccatca cgtgtcgatc
      901 tccttggcgg cggaggcgat ggcccgcgcc agcatgacgt tgccgtggtg actcggacgc
      961 tccgcaagca cgtgcgcgcg gatgcggccc ccgaggagcg ccaggtcgcc gaccaggtcg
     1021 cccgccttgt gccggacgaa ttcatcgggc caccgcagaa cggtacccac gacgccccgg
     1081 tcgtccagcc cgatcgcgtt gtcatacgag gcgccgagcg ccagcccgcg tgcctggagc
     1141 gcctccacct cacggaccag cccgaacgtg cgcgcccccg ccagctcccg gcggaacacc
     1201 ccgggggtca gctcccacga gcccgattgg cggccgatgg cgggatgcgc ccactccacg
     1261 gtgacgctga cccgcagcgt ggcggacggc tccgcccggt agcgcgatcc ggccaccacg
     1321 acctcgaggg gcgccggcac ccgccattcg gcgggggtcc cggcctggtc ggccagcccg
     1381 gcgtcgagca gcccctcgac gaagggggcg aacgaaccgt cgaggatcgg gggctccggg
     1441 ccgtcgagct ccaccagtgc gtcgtccacc tcgagcgcct ggagcgccgc gagcaggtgc
     1501 tcgacggtgt ggatggtggc cggcgggcgg ccgatggcgg tccgccgttc ggtggcctcc
     1561 acttccgcaa gccgggccgg gatgcgtggc acgccggcca ggtccgtccg ggcgaacacg
     1621 atgccggtcc ccgtccccgc cgggccgacc cggagcctgg tctcgacccc cgtgtgcagc
     1681 gccgtccccc ggaactcggc cgggcgggcc agcgtccgcc taggcatcgg gtcgggcgcg
     1741 ctcgaccagc cgctcgagct tggcgctgat gccggtgagt cggccgagtg ccgcctgccg
     1801 gcgcagcgtc tcctgctgcg ggcgcgccgg caggccgccc cacgtctccc cggccggcac
     1861 gtcgccccag acggcgctga accccgcgat ccgggcgccg tcgccgaccg tccggtggcc
     1921 gttgacggcg gtatggccgc cgatgaggac gccgtcgccg acgcggacgc tccccgcgaa
     1981 cccgcactgc gcggccacca ggcaccgccg cccgatccgg acattgtggc cgacgtgcac
     2041 catgttgtcg agcttggtgc catcgccgag gaccgtgtcg tccaggctgc cgcggtcgac
     2101 cgtcgcgttg gcgccgacct cgacgtcgtc gccgatgatg caccgcccga tgtgcacccg
     2161 ccgatccggc accgccgcgc cttccatgaa cccgaaaccc ggcgagccga tgaccgcgcc
     2221 tgccttgatc acgacccggc ggccgatgat cgaccccggg tacacggtga cccccgggtc
     2281 gatgaccgtg tcgtcgccaa cggtgacgcc atcaccgagg atgacgtgcg cccccagaat
     2341 gacccgcgcg ccgacccggg agtcccggcc aaggacgacg tacggcgcga gcgacgcgcc
     2401 ggccccgatg ctcgcgccgg ggccgaggat cgcgctcggg tggatgccgg gctccggcgc
     2461 caccgtggga tagagcaccg ccgccaccgc ctggaccgcg cggtacgggt ccgccacgac
     2521 gatccggcag gccggccccg ccgcctcggc ggcgtactgc tccggcagca ggaccagccc
     2581 cgctcgcgag gcacggaacg ccggcacgta tcggggtccc gtgaggaacg acagtgtctc
     2641 gggacccgca cggtcgagcg gaccgatcgc ggtgaccgcg gccccgcccg cgccgacgag
     2701 acggccgccg accagctcgg cgaccgcctc ggcggtgagc ggggcccggt cggacgccat
     2761 ctacttggcg gcctgcagcc ggtcgatcac ggtctgggtc aggtcgagcg agcgatccgc
     2821 cgcgaccacg gcgccgccca tggccgacac gtcgaagatc atcgcgtagt tgccctccgc
     2881 gcggatcccc tcgatcaccg cctgtacccg ggcctggatc ggctcgatca gctcgcgctc
     2941 ccggctctgc atccgggtct ggagctcctg ggtcttctgc tcgatgtcct gctgctgctg
     3001 caccagtccc tgctgcttct gctggcgcgc cgtcggcgac agaagcaccg acgaggtctg
     3061 gaattcgttg gcggcc
//
LOCUS       DASUAP010000221         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1259997, whole genome shotgun sequence.
ACCESSION   DASUAP010000221 DASUAP010000000
VERSION     DASUAP010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259997"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFU45_05715"
     CDS             complement(<1..690)
                     /locus_tag="VFU45_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5358591.1"
                     /translation="MTQPGLFSTPEPLAARLRPRTLEEYVGQAQLLAPGRALGDAIRR
                     GDVGSIILWGPPGSGKTTLARLIAQYTSRHFTAFSAVTEGVPRVREIIAEAAARRSEE
                     GKGTILFCDEIHRFNRAQQDAFLPHVESGVITLIGATTENPSFELTGALLSRSRVYVL
                     EPLGADDLRELVRRALSDRERGLGTLELTLDPAAEGALVAIADGDARRVLTALEAAAQ
                     QAGLKGTITLAM"
     gene            805..1203
                     /locus_tag="VFU45_05720"
     CDS             805..1203
                     /locus_tag="VFU45_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEU5358592.1"
                     /translation="MAAPSRFPSLDADDLNTTGRVIGAAIRVHRALGPGLLEGLYHRA
                     FAVELSQSGIRHDVERPVVLTYAGVKIGTQRLDIVVEDRLVVELKAVEALSRLHRAQL
                     LSYLRATRIRIGLLMNFNSEVLTVKRILNG"
     gene            complement(1466..2179)
                     /locus_tag="VFU45_05725"
     CDS             complement(1466..2179)
                     /locus_tag="VFU45_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEU5358593.1"
                     /translation="MTPIVWALLAAAGNLVGAIVVVRHRPEQVRLLETFIAIGAGFML
                     TVALGEVVPEAFARDASGAAVYLVAGYFAVHLAQHVLTPHFHFGEETHAVSPVASVSA
                     LLGLLLHTFFDGVAIASGFSVSHTLGVLLFTAVFLHKLPEGLAMASLMLASGLGRARA
                     IGAAAALGVATLVGVLATDLVAPLRDHGLALAGGVTIYVAASNLVPEIQSRRGWRMPL
                     SFFGGAAAFFAARALLGGG"
     gene            complement(2176..>2381)
                     /locus_tag="VFU45_05730"
     CDS             complement(2176..>2381)
                     /locus_tag="VFU45_05730"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HEU5358594.1"
                     /translation="PAMQRILDRGDPIDRGALAVTGADLLDAGIPKGPALGKVLKGLL
                     DFVLEDPSRNTREVLLRQAKALS"
BASE COUNT          370 a          828 c          835 g          348 t
ORIGIN      
        1 catcgccagc gtgatcgtcc ccttgagccc cgcctgctgc gccgccgcct cgagcgcggt
       61 gaggacccgc cgcgcgtcgc catcggcgat ggccacgagc gccccctccg ccgcgggatc
      121 gagcgtgagt tcgagcgtgc caagcccccg ctcgcggtcg ctgagggcgc ggcggaccag
      181 ctcgcgcagg tcgtcggcgc ccagtggctc gaggacgtag acgcgggagc gggagaggag
      241 ggcgccggtg agctcgaagc tcgggttctc ggtggtggcg ccgatgaggg tgatgacacc
      301 gctctcgacg tgcggcagga aggcatcctg ctgcgcccgg ttgaagcggt ggatctcgtc
      361 gcagaagagg atcgtcccct tcccctcctc gctccgccgc gccgccgcct cggcgatgat
      421 ctcccgcacc cgggggaccc cttcggtgac cgcgctgaag gcggtgaagt gccgcgaggt
      481 gtactgcgcg atcaggcgcg ccagcgtcgt cttccccgag cccggcggcc cccagaggat
      541 gatgctcccg acgtcgcccc gccggatcgc atcgcccagc gcccgccccg gcgccagcag
      601 ctgcgcctgg ccgacgtact cctccagcgt gcggggccgg agccgcgccg ccaggggttc
      661 gggggtagag aataggccgg gttgggtcat cgcgctcgaa cgatcagcgt cgttccgttc
      721 tggttatcgg ggtcccgggt ggccaccgca tcttgtcgcg aacacggaat cactgagcct
      781 tgaagaatca ccgaaagtcg cattgtggca gctccgtcga gattcccgtc gctcgatgcc
      841 gacgacctga acaccacagg cagggtgatc ggagcggcca tccgggtgca tcgtgcgctg
      901 gggcccgggc ttcttgaggg gctctatcat cgcgcgttcg ccgtcgagct cagccaaagc
      961 ggcatcaggc acgacgtgga gcgcccggtc gtcctgacgt atgctggcgt caagatcggg
     1021 acgcagcgcc tcgatatcgt ggttgaagac aggctcgttg tcgaactcaa ggcagtcgag
     1081 gcgctctcgc gattgcatcg ggcccaattg ctgagctatc tccgggccac gcgcatccgg
     1141 atcggccttc tgatgaactt caacagcgaa gtcctgacgg tgaaacggat cctgaacggt
     1201 tgaccacacc aagctcgcgg tgtttccggg ggacttccgc gcttccgtgt tcgcactgcg
     1261 ctcagctacg acggcgacac ctatcgttca ccgcggagct cggcgcgaac acggaaccat
     1321 ggaaggacca tcgaaacacc gaaggccgct tgcgtggggg gacattcgag atcccgtccg
     1381 ttcacccaca tcggatgtgc cgtgcttcgg gtgcgtgtcc gtgattccgt gtccgcacgt
     1441 gatccggtcg gtgccgaacg ccgattcagc ccccccccag cagcgcccgc gccgcgaaga
     1501 acgccgcggc gcctccgaag aacgacagcg gcatccgcca cccgcggcgc gactggatct
     1561 ccggcaccag gttcgaggcc gccacgtaga tcgtgacccc gcccgccagc gccagtccgt
     1621 ggtcccggag cggggccacc aggtccgtcg ccagcacccc cacgagcgtc gcgacgccga
     1681 gcgccgccgc cgccccgatc gcgcgggcgc ggccgagccc cgaggcgagc atcaggctcg
     1741 ccatcgccag cccttcgggg agcttgtgga ggaagacggc ggtgaagagg aggacgccga
     1801 gcgtatggga gacgctgaac ccgctcgcga ttgcgacgcc gtcgaagaag gtgtggagca
     1861 ggaggccgag gagcgcgctg acgctcgcca ccgggctcac cgcgtgggtc tcttcgccga
     1921 agtggaagtg gggcgtcagg acgtgctggg ccaggtgcac cgcgaagtag ccggccacga
     1981 ggtagacggc cgctccgctc gcgtcccggg cgaaggcctc cggcaccacc tcgcccagcg
     2041 ccaccgtcag catgaacccg gcgccgatgg cgatgaacgt ctcgaggagc cggacctgct
     2101 ccggccggtg gcgcaccacg acgatggcgc ccacgaggtt gccggcggcg gcgagcagcg
     2161 cccacacgat cggggtcacg agagggcctt ggcctggcgg aggagcactt cacgggtgtt
     2221 gcgcgacggg tcctccagca cgaagtcgag gagtcccttg agcaccttgc cgagcgccgg
     2281 ccccttgggg atcccggcat cgaggaggtc cgcgccggtc acggcgaggg cgccgcggtc
     2341 gatcgggtcg ccccggtcga ggatccgctg catcgccggc t
//
LOCUS       DASUAP010000222         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_456467, whole genome shotgun sequence.
ACCESSION   DASUAP010000222 DASUAP010000000
VERSION     DASUAP010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456467"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VFU45_05735"
     CDS             <1..749
                     /locus_tag="VFU45_05735"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5358595.1"
                     /translation="VSALSAGELRALHGRGYYPGNVVVAAAGHLDHDQVLGLLEHEGW
                     LEPGSAAPRDAVAPAPARRGVHQRHERDTTQTHIVVGTDTFASRDPRRWALSVVTNVF
                     GGGMSSRLFQRLREELGLAYAVYAFQSLYQASGLAGSYIGTQPATADQALEAILAEYG
                     RLAREGLTAQELSDGKQQLKGQIMLGLESPLSRMGRMASVALAQDRYRDLDQLLAEVD
                     AITADDAAAIAAEYFAPERQTVVRLGPGGR"
     gene            875..1987
                     /gene="ald"
                     /locus_tag="VFU45_05740"
     CDS             875..1987
                     /gene="ald"
                     /locus_tag="VFU45_05740"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU5358596.1"
                     /translation="MQIGVPKEIKTNENRVALVPAGAEALVAGGHTVTVEQGAGVGSG
                     FADEAYAAAGAKIIPTADEVWAKADMIMKVKEPIASEWPRMRKGQVIYTYFHFAAAEE
                     LTKAVIKSGAIAVAYETVQLPSGELPLLTPMSEVAGRMAVQEGAKYLEKHHGGSGILL
                     GGVPGVAPAEVTIIGGGVVGINAAKMAAGLGAHVTILDVSLERLRYLADVLPANVDTL
                     FSNRHNILEAIRRSDLVVGAVLLPGAKAPHLVKRDDLRLMKQGSVIVDVAVDQGGCVE
                     TIKPTTHENPTYFVDGILHYAVANMPGGVPRTSTLALTNATLKYGVRLARDGWRKACR
                     EDRALALGLNVVEGKVVYPGVAEAFGLPLADVASVL"
     gene            2086..>2504
                     /gene="dut"
                     /locus_tag="VFU45_05745"
     CDS             2086..>2504
                     /gene="dut"
                     /locus_tag="VFU45_05745"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU5358597.1"
                     /translation="MLDLQFTRLRHAEGLPVPSRQTDGAAGYDIVAAEEVTFGPMERK
                     LVPTGFCIAIPAGYECQIRPRSGLAWKHGMTLPNTPATIDSDYRGEIQVPMINLGQEP
                     FTVTRGMRIAQLVFARFEVAEFREVGELPATGRGAGG"
BASE COUNT          383 a          853 c          927 g          341 t
ORIGIN      
        1 ccgtctccgc gctctcggcc ggggagctcc gcgccctcca cgggcgcggc tactatcccg
       61 gcaacgtggt ggtggccgcc gcgggccacc tcgaccacga ccaggtcctt ggcctcctcg
      121 agcacgaggg atggctggag ccggggtccg cggccccgcg tgatgccgtg gccccggcgc
      181 cggcgcgccg gggggtccac cagcggcacg aacgggacac gacccagacc cacatcgtcg
      241 tgggcaccga taccttcgcc tcccgcgacc cgcgccggtg ggcgctctcg gtcgtgacca
      301 acgtcttcgg cggggggatg tcgagccggc tcttccagcg cctccgcgaa gagctcggcc
      361 tcgcgtacgc cgtgtacgcc ttccagagcc tctaccaggc gtcggggctg gccgggagct
      421 acatcggcac ccagcccgcc accgccgacc aggcgctcga ggcgatcctg gccgaatacg
      481 gacggctcgc gcgcgagggg ctcaccgccc aggagctgtc ggacggcaag cagcagctca
      541 agggccagat catgctgggg ctggagagcc cgctgagccg gatggggcgg atggcgtcgg
      601 tggcgctggc gcaggaccgc tatcgcgacc tggaccagct gctggccgag gtcgatgcca
      661 tcaccgcgga cgacgcggcc gccatcgcgg ccgagtactt cgctcccgag cgccagacgg
      721 tggtgcggct ggggccgggg ggccggtgag cagtggacgg cgaacagtga acggtgaagc
      781 gtgaacggtg aatggagaac ggcacgggat gtcccgttga ccgttcaccc ttgaccgttc
      841 accggcagtc aaactccaat tcgcagagac gaccatgcag atcggcgtgc cgaaggagat
      901 caagaccaac gagaaccggg tggcgctggt gccggcgggg gcggaagccc tcgtggcggg
      961 cgggcacacg gtcaccgtgg agcagggcgc cggcgtcggg agcggcttcg ctgacgaggc
     1021 ctacgcggcc gccggggcga agatcatccc gaccgccgac gaggtgtggg ccaaggccga
     1081 catgatcatg aaggtgaagg agccgatcgc gtcggaatgg ccgcggatgc gcaagggaca
     1141 ggtcatctac acctacttcc acttcgcggc ggccgaggag ctcaccaagg cggtgatcaa
     1201 gtcgggcgcg atcgcggtgg cgtatgagac cgtccagctg cccagcggcg agctgcccct
     1261 gctcacgccg atgtcggaag tggcgggccg gatggcggtg caggaggggg ccaagtacct
     1321 cgagaagcac cacggcggga gcggcatcct gctgggtggc gtcccgggcg tggcgcccgc
     1381 cgaggtgacg atcatcggcg gcggcgtcgt ggggatcaac gcggccaaga tggccgccgg
     1441 ccttggcgcc catgtgacga tcctcgacgt gtcgctcgag cggctgcggt acctcgcgga
     1501 cgtcctgccg gccaacgtcg atacgctctt ctccaaccgc cacaacatcc tcgaggcgat
     1561 ccgccggtcg gacctcgtgg tcggggcggt gctgctcccg ggcgccaagg ccccgcacct
     1621 cgtcaagcgc gacgacctcc ggctcatgaa gcagggctcg gtcatcgtgg acgtggcggt
     1681 ggaccagggg ggctgcgtcg agaccatcaa gcccaccacg cacgagaacc cgacctactt
     1741 cgtggacggg atcctgcact acgccgttgc caacatgccc ggcggcgtgc cgcgcacttc
     1801 gacgctggcg ctcaccaacg cgacgctcaa gtacggcgtc cggctggccc gggacggctg
     1861 gcggaaggcc tgccgcgagg accgcgccct ggcgctcggc ctcaacgtgg tcgagggcaa
     1921 ggtggtctac cctggcgtgg ccgaggcgtt cgggctcccg ctggcggacg tggcgtcggt
     1981 gctgtagcgg cgcagggcgg gagggacgca ggggcgcacg gtctccgccc ctgcgttccc
     2041 gatctcctgc gccactgcga ccctgcgccg ctgcgccaat caccgatgct cgacctccaa
     2101 ttcacccgcc tccggcacgc cgaggggctc ccggtgccct cccggcagac cgatggggcc
     2161 gcggggtacg acatcgtggc tgcggaagag gtcaccttcg gcccgatgga gcggaagctc
     2221 gtgcccacag ggttctgcat cgcgatcccg gcggggtacg agtgccagat ccgcccccgc
     2281 tcggggctgg cctggaagca cgggatgacg cttcccaaca ccccggccac gatcgacagc
     2341 gattaccggg gggagatcca ggtcccgatg atcaacctgg ggcaggagcc cttcaccgtg
     2401 acccggggga tgcggatcgc ccagctggtc tttgcccggt tcgaggtggc ggagttccgg
     2461 gaggtcgggg aactcccggc gacggggcgg ggggcggggg ggtt
//
LOCUS       DASUAP010000223         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_401288, whole genome shotgun sequence.
ACCESSION   DASUAP010000223 DASUAP010000000
VERSION     DASUAP010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401288"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFU45_05750"
     CDS             <1..198
                     /locus_tag="VFU45_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358598.1"
                     /translation="FQVLQRAGQVSTEEMRDVFHLGVGLITVLDPGDVAAAQAAARAA
                     GIDTWILGEVRRGDRTVRFSA"
     gene            220..1188
                     /locus_tag="VFU45_05755"
     CDS             220..1188
                     /locus_tag="VFU45_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358599.1"
                     /translation="MRRQLVVAGLAVLFAAPALRAQNAECAPYSASTTATNLCNAGVD
                     ATRYFHPLLGLVTSGGDPVLGTAKTLGGFPHFSIGLRVNATSLTLPDLSYNGSSDTVA
                     KADKIPLPAPVVEGALGVYKGLPAGLLSVDVLASATLLPVGAVKDLHVDPSATKIGDV
                     VLGMGYGVRVGILKGSFPIPSVSASVMRRSIPRIQYGDPANDASQNFGYSLDLHATSW
                     RIAASTKLVFLDLTAGAGYTTYTGDAQILFRDPVIATQIDSVNVKLDQGRGMVFADAG
                     VDLFVMKLVGEIGWESAGNLTPSTTFQDVDPKAGHLFGGLGLRISF"
     gene            1190..>2177
                     /gene="ribD"
                     /locus_tag="VFU45_05760"
     CDS             1190..>2177
                     /gene="ribD"
                     /locus_tag="VFU45_05760"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU5358600.1"
                     /translation="MTEAEAMDRALSLAWRGWGRVHPNPLVGAVVLKDGAVVGQGWHA
                     EFGGPHAEVAALTEAGGAAKGATLVVTLEPCAHHGKQPPCTDAIQRAGIRRVVAAMAD
                     PNHLAGGGAAVLRARQVEVDIGLRAAEACRQNAPFRWRYSSSARPWVALKLATSLDGA
                     IADASGRSRWVSGPEAREYAHWLRAGFDALAVGGATARADDPALTVRGSVVPRIAPRR
                     VVFDRGGRLPPELKLVRTAREQRTILVTSAALAAAPAARELERLGVAVLGSGSLEEGL
                     RQLREQEDVHSIMVEGGGRLAGALLDAGLVDRLYWIQAPVWLGQGAVAAFAGL"
BASE COUNT          279 a          800 c          788 g          310 t
ORIGIN      
        1 ttccaggtcc tgcagcgggc ggggcaggtc tccaccgagg agatgcgcga cgtgttccac
       61 ctcggtgtcg ggctcatcac ggtgctggat cctggcgacg tcgcggcggc ccaggccgcc
      121 gcgcgtgccg ccgggatcga tacgtggatc ctcggcgaag tgcgccgggg cgaccgcacg
      181 gtgcggttct ccgcgtgacc cctgaccgga ggtagctcca tgcgcaggca actcgtggtc
      241 gcgggactgg cggtcctgtt cgcggcaccg gcgctccggg cgcagaacgc cgagtgcgcc
      301 ccgtacagcg ccagcaccac cgcgaccaac ctctgcaatg ccggcgtcga cgccacgcgg
      361 tacttccacc cgctcctggg cctcgtcacc agcggcggcg atcccgtcct gggcacggcc
      421 aagacgctgg gcggcttccc ccacttctcg atcgggctcc gggtcaacgc gaccagcctc
      481 acactcccgg acctgagcta caacggctcc tccgacaccg tggccaaggc ggacaagatc
      541 ccccttcccg cgccggtcgt ggaaggtgcg ctcggcgtgt acaaggggct tcccgccggg
      601 ctcctgtcgg tggacgtgct ggccagtgcc acgctgctgc cggtgggcgc cgtcaaggac
      661 ctgcacgtcg acccgagcgc caccaagatc ggcgacgtcg tgctcgggat ggggtatggt
      721 gtccgggtcg ggatcctcaa ggggagcttc ccgatcccgt cggtctcggc gtccgtgatg
      781 cgccggagca tcccccggat ccagtacggc gaccccgcca acgacgcgag ccagaacttc
      841 ggatactcgc tcgacctcca cgccaccagc tggcggatcg ccgcgagcac caagctcgtc
      901 ttcctcgacc tcaccgccgg cgccggttac accacctaca ccggcgacgc gcagatcctc
      961 ttccgcgacc cggtgatcgc gacccagatc gactcggtca acgtgaagct cgaccagggc
     1021 cgcggcatgg tgttcgcgga cgcgggcgtc gacctcttcg tgatgaaact ggtcggcgag
     1081 atcgggtggg agagcgccgg caacctgacc ccgtccacca ccttccagga cgtcgacccg
     1141 aaggcggggc acctgttcgg cgggctcggg ctgcgcatct ccttttagcg tgaccgaagc
     1201 cgaagcgatg gaccgcgccc tcagtctcgc ctggcgaggc tgggggcgcg tgcatccgaa
     1261 ccccctggtc ggcgcggtcg tcctcaagga cggcgcggtc gtgggccagg ggtggcacgc
     1321 cgagttcggg gggccccacg ccgaggtggc ggcgctcacc gaggccggtg gggcggcgaa
     1381 gggggccacg ctcgtcgtga cgctcgagcc ctgcgcccac cacggcaagc agccgccctg
     1441 caccgacgcg atccagcgcg ccggcatccg ccgggtagtg gccgccatgg ccgaccccaa
     1501 ccacctggcc gggggcggcg cggcggtgct tcgggcgcgt caggtcgagg tggacatcgg
     1561 cctgcgggcg gcggaggcgt gccgccagaa tgcgccgttc cggtggcggt actcctcgtc
     1621 cgcgcgcccc tgggtggcgc tcaagctcgc cacctcgctc gacggcgcca tcgccgacgc
     1681 ctcgggtcgc tcccgctggg tgtcggggcc ggaggcgcgg gaatacgcgc actggctccg
     1741 ggccgggttc gacgccctcg cggtcggcgg cgccaccgcc cgggccgacg accccgccct
     1801 cacggtgcgg ggctcggtgg tcccgcgcat cgcgccgcgg cgggtggtct ttgaccgggg
     1861 gggccgcctg ccgccggagc tcaagctcgt ccgcaccgcc cgcgaacagc gcaccatcct
     1921 ggtcacctcg gccgccctgg ccgccgctcc cgcggcccgg gagctcgaac ggctcggtgt
     1981 cgccgtcctt gggagtggct cgctggagga ggggctccgg cagctccggg aacaggagga
     2041 tgttcactcg atcatggtgg aggggggcgg acggctcgcc ggggcgctgc tggacgcggg
     2101 gctcgtcgac cgcctctact ggatccaggc cccggtgtgg ctcggacagg gcgcggtggc
     2161 ggcgttcgcc gggctcg
//
LOCUS       DASUAP010000224         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1000876, whole genome shotgun sequence.
ACCESSION   DASUAP010000224 DASUAP010000000
VERSION     DASUAP010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000876"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFU45_05765"
     CDS             <1..640
                     /locus_tag="VFU45_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU5358601.1"
                     /translation="GTQMLLGTLVVPDASTWAFQTLKPGSVSPLIETSDAIFLFRIDS
                     IAPAGVPPLDRIRLAVETAAREAKKITMARDIGQDLLKRMNEGSSLDQAADAHHLPHK
                     IMGPFTRTKPDIGNPLVVGTAFGLAVGQRSPLLDTPDGIYLFDVTAHQPADSADFVKN
                     QAQLMFTAMSRARQDRARAYLQALRTSAKIVDNRAKLFQPAQQQQQAADQPI"
     gene            complement(746..997)
                     /locus_tag="VFU45_05770"
     CDS             complement(746..997)
                     /locus_tag="VFU45_05770"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5358602.1"
                     /translation="MFERIGFSEILLIGALFFLFFGAKRLPEMGSSIGKGIREFKRSL
                     SEINDDLPASKPEQYSQAPRQLDAPANPPSGGEPKRLSQ"
     gene            complement(1009..1281)
                     /locus_tag="VFU45_05775"
     CDS             complement(1009..1281)
                     /locus_tag="VFU45_05775"
                     /inference="COORDINATES: protein motif:HMM:NF025572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HEU5358603.1"
                     /translation="MPAGPRRPGFYLSVLTLGFLVGGFLNSLLSYFLPDSAAKDFFTF
                     TVTPTLGPLSVNLLVISFTLGPVGLHVSLLSLVGVVLAYLLARSLF"
     gene            complement(1281..>2031)
                     /locus_tag="VFU45_05780"
     CDS             complement(1281..>2031)
                     /locus_tag="VFU45_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5358604.1"
                     /translation="LGHGAHIGQPRHIGQDQGLRRGMPTINALFSHQVSVIMGDYLYS
                     RAVVELVALRDLDALDVVGRVTNDMTVGEMRQLLAHDGLAFTEEAYDLLIRSKTASLL
                     AGACEVGALQAGPEQRSALRRFGQELGMAFQIVDDLLDYAGDASETGKPVGGDLREHK
                     VTLPLIAALPRFSAADRRVLQDLMREAEPAEERIAAVIQAVTAAGGLEYARERAHRHA
                     AAAEAALDVLPASVYRDALRGSIGYVLERRR"
BASE COUNT          336 a          741 c          689 g          265 t
ORIGIN      
        1 ggggacccag atgctcctcg gcaccctggt ggtgccggac gccagcacct gggcgttcca
       61 gaccctcaag ccggggtcgg tgagcccgct catcgagacc agcgacgcca tcttcctctt
      121 ccgcatcgac agcatcgcgc ccgccggcgt gccgccgctc gaccggatcc ggctcgccgt
      181 cgagaccgcc gcgcgcgagg cgaagaagat cacgatggcg cgcgacatcg gacaggacct
      241 gctcaagcgg atgaacgagg ggagcagcct cgaccaggcc gccgacgcgc accacctgcc
      301 ccacaagatc atggggccgt tcacccgcac gaagccggac atcggcaacc cgctcgtggt
      361 gggcaccgcc ttcggcctcg cggtcgggca gcggagcccg ctcctcgaca cgccggacgg
      421 catctacctg ttcgacgtca cggcccacca gccggccgac tcggccgatt tcgtcaagaa
      481 ccaggcccag ctgatgttca cggcgatgtc gcgggcgcgg caggaccggg cgcgcgccta
      541 cctccaggcg ttgcgcacca gcgccaagat cgtcgacaac cgcgccaagc tcttccagcc
      601 cgcgcagcag cagcagcagg cggccgacca gcccatctga ccgacggatc cgccgcgacc
      661 acggcgcgcg tggtcgtggc cttcaacgac aacgggcggc cctccccggc ttcggggggc
      721 cgcccgttcc gttgccaggc gtcagctact gcgagaggcg cttcggctca ccgcccgagg
      781 gcggattggc cggcgcgtcg agctggcgcg gggcctggga gtactgctcc ggcttcgacg
      841 cgggaaggtc gtcgttgatc tcggagagac tccgcttgaa ttcccggatc cccttgccga
      901 tcgaggaccc catctccggc aggcgcttgg ccccgaagaa gaggaagaac agcgccccga
      961 tgagcaggat ctccgagaac ccgatccgtt cgaacatatg ccaccccttc agaagagcga
     1021 acgagccaac aggtaggcca gcaccacgcc gaccaggctg agcagcgaga cgtgcagccc
     1081 caccggaccc agggtaaagc taatcacgag caggttcacc gacagcggcc ccagggtggg
     1141 ggtcaccgtg aaggtgaaga agtccttggc cgcgctgtcg ggcaggaaat atgacagcag
     1201 cgagttgagg aaccccccga ccaggaaccc cagcgtgagc acgctgaggt agaagccggg
     1261 ccgtcgcggt cccgccggca tcagcgcctc cgctccagga cgtagccgat gctcccgcgg
     1321 agcgcgtcgc gatagaccga ggccggcagc acgtcgagcg ccgcctccgc ggcggccgcg
     1381 tggcggtggg cccgctcccg ggcatactcg agcccgccgg ccgccgtcac cgcctggatg
     1441 accgccgcga tccgctcctc cgccggttcg gcctcccgca tcaggtcctg cagcacccgc
     1501 cggtcggcgg cgctgaagcg cgggagggcg gcgatgagcg gcagggtcac cttgtgttcg
     1561 cggaggtcgc ccccgaccgg cttgccggtc tcggaggcat cgccggcgta gtcgagcagg
     1621 tcgtcgacga tctggaacgc catccccagt tcctggccga agcgccggag cgccgagcgc
     1681 tgctccggcc cggcctggag cgcgccgacc tcgcacgcgc ccgcgagcag cgatgccgtc
     1741 ttggagcgga tgaggaggtc gtaggcctcc tcggtgaagg ccagcccgtc gtgggccagg
     1801 agctggcgca tctcgcccac ggtcatgtcg ttggtgaccc ggcccaccac gtccagcgca
     1861 tcgaggtcgc ggagcgccac cagctccacc acggcgcggg agtacaggta gtcgcccatg
     1921 atgaccgaca cctggtggct gaacagcgcg ttgatcgtcg gcatcccgcg gcggagccct
     1981 tgatcctggc caatgtgccg cggctggccg atgtgcgcgc catggccgag c
//
LOCUS       DASUAP010000225         5982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1150820, whole genome shotgun sequence.
ACCESSION   DASUAP010000225 DASUAP010000000
VERSION     DASUAP010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150820"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFU45_05785"
     CDS             <1..470
                     /locus_tag="VFU45_05785"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358605.1"
                     /translation="CAEPAPGERVWDLYAGIGETTELLLEQGAVVDSVEIDRRAVEQG
                     LRRSRFGPPVGSQAPGGPVARHAGRVEDQAGRLPRPAVVVTNPPRVGMEPAAVAAIAA
                     ARPRRIVYVSCDPATLARDVARLGTGYRVRSIRAFDLFPQTAHVETVLVLEAA"
     gene            467..979
                     /locus_tag="VFU45_05790"
     CDS             467..979
                     /locus_tag="VFU45_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU5358606.1"
                     /translation="MKYFVDVGGREFVVEVDGGRVTLDGIVVEAHLSRLEGTPLWHLL
                     LGDASCTLVLDADGRGRWRAERFGERWEVEVVDERTRHVRSLTGGGMKAAGPPVLRAP
                     MPGLVVRVPVAVGDIVAAGAGVVVLEAMKMENELRTQAGGEVSAVHVRPGQAVEKGQV
                     LVEFAPTPLP"
     gene            1054..1515
                     /gene="rplM"
                     /locus_tag="VFU45_05795"
     CDS             1054..1515
                     /gene="rplM"
                     /locus_tag="VFU45_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU5358607.1"
                     /translation="METFSAKPKDLSHRWLLVDATDVPLGRLASRVAQLIRGKHKPTF
                     TPHMDGGDFVVVVNASKVKLTGRKLSDKTVFSHTGYMGHESHTPIRNILAKHPDRVIE
                     KAVFGMLPKNSLAKQKLRTKLKVYGGAEHPHQAQAPEPTSVAPTAKKKVTA"
     gene            1512..1913
                     /gene="rpsI"
                     /locus_tag="VFU45_05800"
     CDS             1512..1913
                     /gene="rpsI"
                     /locus_tag="VFU45_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU5358608.1"
                     /translation="MTAKSEFRWQAVGRRKTSVARVYLTPGTGKWDVNGRTLGDYFPR
                     PSLVQHIQQSFTVTDTLGAFDVKAHVDGGGQSGQAGALRLGIARALISADSQHRAKLR
                     TAGLLTRDPREVERKKPGRPGARKRFQFSKR"
     gene            2067..2951
                     /gene="rpsB"
                     /locus_tag="VFU45_05805"
     CDS             2067..2951
                     /gene="rpsB"
                     /locus_tag="VFU45_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU5358609.1"
                     /translation="MAQPQLQDLLEAGVHFGHQTRRWNPKMRRFIFAERSGIYIIDLQ
                     KTLRQIQQAQELLKSVVLKGEAVLFVCTKKQLKGIVQAEAQNSNSFYVTERWLGGTLT
                     NFLTVKKQLRRLRDLEQGAAEGDFEHYTKKEQLLFEREREKLSRNLEGIKHMARLPGA
                     LFVVDAKKEKIAVSEANKLGIPVVAIVDTNADPDVITVPIPGNDDAIRAVSLVTSALS
                     DVLKEARAQMPLREVVEDGEGETYSTDGGTEGDADSERRRRRPRRKRRPKPEAIAARL
                     KTDAVDGEPGDATAASDA"
     gene            3048..3911
                     /gene="tsf"
                     /locus_tag="VFU45_05810"
     CDS             3048..3911
                     /gene="tsf"
                     /locus_tag="VFU45_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU5358610.1"
                     /translation="MATKAITPKDVSELRARTGAGMMDCKKALEETGGDMDQAAEYLR
                     KKGIAKAEKRAGRDAMQGLIFLNAAEDGDFGTMVELNCETDFVARTEGFQTLGLSLLA
                     FAATAPVGAHAVGADGALLAQPFEGKTLGERVKEVAGVIGEAISVKRLAHFRPRGGVV
                     GSYLHHNRQVGVLVELDGVTGDAALALAKDVALHIASADPIGVSAQDIPKELMDRERR
                     IAEEQVAQEGKPEQIRAKIVEGKLKKFAQERSLLDQPFVKDETKTVADLLKAVPGARV
                     VRFARFRIGDA"
     gene            3911..4621
                     /gene="pyrH"
                     /locus_tag="VFU45_05815"
     CDS             3911..4621
                     /gene="pyrH"
                     /locus_tag="VFU45_05815"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU5358611.1"
                     /translation="MALAYRSALLKVSGEALAGDKGQGLDFRVVGALADAIKDVHALG
                     VRLALVVGGGNIIRGATASRDGLDRVSADYMGMLATVINALAVQDVLEKKGVDTRVMT
                     AIRMESLAEPYIRRRAQRHQEKGRLVLFAGGTGNPFFSTDTAAVLRALEVEAEVILKG
                     TNVDGIYTGDPRKDPKARFIPELSYQDAIVNNYEVMDANAFGLCKSNNLPIVVFNISQ
                     PGAITRILQGERLGTLVR"
     gene            4618..5172
                     /gene="frr"
                     /locus_tag="VFU45_05820"
     CDS             4618..5172
                     /gene="frr"
                     /locus_tag="VFU45_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU5358612.1"
                     /translation="MSTIPETTRHAREAMTKAIESVKRELSGIRSGKASPSLLDTVRV
                     DAYGTVVPLNQVALVAAPEPRLLTVQPFDKGLASAVEKAIRDSGLGLNPATQGSIIRV
                     PLPQLTEERRKELVKLVHKLAEEGRVAVRQARGEAHAKVKKLQGLPEDDVKRAEKEVQ
                     KLTDDAVRQVDQLVAGKEKELMEV"
     gene            5174..5917
                     /gene="uppS"
                     /locus_tag="VFU45_05825"
     CDS             5174..5917
                     /gene="uppS"
                     /locus_tag="VFU45_05825"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU5358613.1"
                     /translation="MTHDDLLDRLRLQGGIPAHVAIIMDGNGRWATGRSLPRSQGHYA
                     GMGAVREAVEGALAAGVEVLTLFAFSQENWQRPADEIDALMALLEEYIAKEAQELRQA
                     GVTVRMLGDLDRLVPSARQAVDRIERDTQGGEKLALNICISYGSRAELTRAARLLAEQ
                     AARGELDPADIDEAAIARQLYTAPWPDPDLLIRTSGEMRLSNFLLWQLAYAEIHVTSV
                     LWPDFTRTDLFRAILDYQRRERRFGRVSV"
BASE COUNT          969 a         2097 c         2116 g          800 t
ORIGIN      
        1 cgtgcgccga gccggcgccg ggcgagcggg tgtgggatct ctacgccggt atcggcgaga
       61 ccaccgagct ccttctcgag cagggggcgg tcgtggactc ggtcgagatc gaccgccggg
      121 cggtggagca ggggctgcgc cgctcgcggt tcgggcctcc cgtcggcagt caggcgcccg
      181 ggggtccggt ggcgcgccat gccgggcggg tcgaggatca ggccggccgg ctcccgcgcc
      241 cggcggtggt cgtcaccaat ccgccccgcg tcgggatgga gcccgcggcg gtggccgcca
      301 tcgcggcggc gcggccccga cggatcgtct acgtctcgtg cgacccggcc acgctggccc
      361 gggacgtcgc ccgccttggg accggctacc gggtgcgttc gatccgggcc ttcgacctct
      421 ttccccagac ggcccatgtc gagaccgtgc tcgtgctgga ggcggcgtga agtacttcgt
      481 cgatgtcggg ggacgggagt tcgtcgtgga ggtggacggc ggacgggtca ccctcgatgg
      541 catcgtggtc gaggcgcacc tctcccgcct tgaagggacg ccgctctggc atctgctcct
      601 gggagacgcc tcctgtacgc tcgttctcga tgccgatggg cggggccggt ggcgggcgga
      661 acgtttcggg gagcggtggg aggtcgaggt cgtcgacgag cggacccggc acgtgcggtc
      721 gctcaccggc ggggggatga aggcggcggg ccccccggtc ctccgggcgc cgatgccagg
      781 gctggtggtc cgggtgccgg tcgcggtggg ggatatcgtg gcggccgggg cgggggtcgt
      841 ggtgctcgag gccatgaaga tggagaacga gctccggacc caggccgggg gggaggtctc
      901 ggcggtccac gtccgccccg ggcaggcggt cgagaaggga caggtgctgg tggagttcgc
      961 cccgaccccg ctcccttgac ccaacccgtt atgcccggct attttgcagg tctgtcccta
     1021 agtcgcgact tttacccggg gtaagcgcag gtcatggaga cgttctccgc caagcccaag
     1081 gatctttcgc accgctggct cctcgtggac gccacggacg tgccgctcgg ccggttggcg
     1141 agccgcgtgg cgcagctgat ccgcggcaag cacaagccga ccttcacgcc gcacatggac
     1201 ggcggcgatt tcgtcgtcgt ggtcaacgcg tcgaaggtca agctcaccgg gcgcaagctc
     1261 agcgacaaga cggtgttctc ccacaccggc tacatgggcc acgagagcca tacgccgatc
     1321 cggaacatcc tcgccaagca cccggaccgg gtcatcgaga aggcggtctt cgggatgctg
     1381 ccgaagaact cgctcgccaa gcagaagctc cggaccaagc tcaaggtgta cggcggtgcc
     1441 gagcacccgc accaggccca ggcccccgag ccgacgtccg tcgccccgac cgcgaagaag
     1501 aaggtgaccg catgaccgcc aagagcgaat tccgctggca ggccgtcggc cgccggaaga
     1561 cgagcgttgc ccgggtgtac ctgacgcccg gcaccggcaa gtgggacgtc aacggccgca
     1621 ccctcggcga ttactttccc cgtccgtcgc tggtgcagca tatccagcag tcgttcaccg
     1681 tcaccgacac gctgggcgcc ttcgacgtca aggcccatgt ggacggcggc ggccagtccg
     1741 ggcaggccgg cgcgctccgc ctcgggatcg cgcgggcgct catctcggcc gactcccagc
     1801 accgcgccaa gctccgcacc gcggggctcc tcacgcgcga cccgcgcgag gtggagcgga
     1861 agaagccggg ccgtcccggc gcccggaagc ggttccagtt cagcaagcgg taagcttgcg
     1921 cagtccgggg gcgcgcaagg gtgcgccccc atcccgcaca tcgctggacc ttgcgccggg
     1981 tgtcccgggc cggtacggac ccgggatcag cgcaggggac gaaggcgatg gacgcccaac
     2041 cccaacgaag gaccaggacg acctgaatgg cacagcccca gttgcaggac ctgctcgaag
     2101 ccggcgtcca cttcggacac cagacccggc ggtggaaccc gaagatgcgg cgcttcatct
     2161 tcgccgagcg ttcgggcatc tacatcatcg acctccagaa gaccctccgc cagatccagc
     2221 aggcccagga gctgctcaag agcgtggtgc tcaagggcga ggccgtgctg ttcgtctgca
     2281 ccaagaagca gctgaagggc atcgtccagg ccgaggcgca gaacagcaac tcgttctacg
     2341 tgaccgagcg ctggctcggc ggcacgctga ccaacttcct gacggtcaag aagcagctgc
     2401 ggcgcctgcg cgacctcgag cagggcgcgg ccgagggcga tttcgagcac tacaccaaga
     2461 aggaacagct cctcttcgag cgtgagcgtg agaagctctc ccgcaacctc gaggggatca
     2521 agcacatggc ccggctcccg ggcgccctct tcgtggtcga cgcgaagaag gagaagatcg
     2581 cggtctccga ggccaacaag ctgggcatcc cggtcgtggc catcgtcgac accaacgcgg
     2641 accccgacgt catcacggtg ccgatcccgg gcaacgacga cgccatccgc gccgtctcgc
     2701 tcgtgaccag cgccctctcc gacgtgctca aggaggcgcg cgcgcagatg cccctccgcg
     2761 aggttgtcga ggacggcgag ggcgagacct acagcaccga cggcggcacc gagggcgacg
     2821 ccgacagcga gcgccgccgg cgccgtccgc ggcgcaagcg gcggccgaag ccggaggcca
     2881 tcgcggcccg gctcaagacc gacgcggtcg acggggaacc cggcgacgcg acggccgcca
     2941 gcgacgcgta agcggacggg cgttccgtcc ggcagcatcc gccgccgctc gtggtccgtt
     3001 cccggccgtg agcggcggct cgtctatcca cactgagcgt gagcaccatg gcaacgaagg
     3061 cgatcacccc caaggatgtc agcgaactcc gcgcccgcac cggcgcgggc atgatggatt
     3121 gcaagaaggc cctcgaggag accggcggcg acatggacca ggccgccgag tacctccgca
     3181 agaagggcat cgccaaggcc gagaagcgcg ccggccgcga tgcgatgcag ggccttatct
     3241 tcctcaacgc cgccgaggat ggcgatttcg gcacgatggt cgagctcaac tgcgagaccg
     3301 acttcgtggc ccgcaccgag gggttccaga cgctggggct cagcctcctc gcctttgcgg
     3361 cgacggcgcc ggtcggcgcc cacgcggtcg gggccgacgg cgcgctcctc gcccagccct
     3421 tcgaggggaa gaccctgggt gagcgggtca aggaagtggc cggcgtcatc ggcgaggcca
     3481 tcagcgtgaa gcggctggcg cacttccgcc cccggggcgg ggtggtcggc tcctacctgc
     3541 accacaaccg gcaggtgggc gtgctggtcg aactggacgg cgtcaccggc gatgccgcgc
     3601 tggcgctcgc caaggatgtg gcgctccaca tcgcctcggc cgacccgatc ggggtctcgg
     3661 cccaggacat cccgaaggag ctgatggacc gcgagcggcg catcgccgag gagcaggtgg
     3721 cccaggaagg gaagcccgaa cagatccggg ccaagatcgt ggaggggaag ctcaagaagt
     3781 tcgcccagga gcggtcgctc ctcgaccagc ccttcgtcaa ggacgagacc aagaccgtgg
     3841 ccgacctcct caaggccgtg ccgggcgccc gggtggtccg gttcgcccgc ttccgcatcg
     3901 gggatgcctg atggcgctcg cctaccgcag cgcgctgctc aaggtgtcgg gtgaggccct
     3961 ggccggcgac aagggccagg gcctcgattt ccgcgtggtc ggcgccctcg ccgacgccat
     4021 caaggacgtc cacgcgctgg gcgtccggct ggcgctggtg gtggggggcg gcaacatcat
     4081 ccggggcgcc accgccagcc gcgacggcct cgaccgcgtc tccgccgact acatggggat
     4141 gctggccacg gtcatcaacg cgctcgcggt gcaggacgtg ctggaaaaga agggcgtcga
     4201 cacccgggtc atgaccgcca tccggatgga gtcgctggcc gagccgtaca tccggcgccg
     4261 ggcccagcgc caccaggaga aaggacgcct ggtcctcttt gccggcggca ccgggaaccc
     4321 gttcttctcc accgacaccg ccgcggtgct ccgggccctc gaggtcgagg ccgaggtgat
     4381 cctcaagggc accaacgtcg acggcatcta caccggcgac ccgcgcaagg acccgaaggc
     4441 caggttcatc ccggagctgt cctaccagga tgccatcgtg aacaactacg aggtgatgga
     4501 cgccaacgcg ttcggcctct gcaagtcgaa caacctcccg atcgtcgtct tcaacatcag
     4561 ccagcccggc gccatcaccc ggatcctgca gggtgagcgg ctcggcaccc tcgtccgatg
     4621 agcacgatcc ccgagaccac ccggcacgcc cgcgaggcga tgaccaaggc gatcgagagc
     4681 gtcaagcgcg agctctccgg catccgcagc ggcaaggcgt cgcccagcct cctcgacacg
     4741 gtgcgggtcg acgcctacgg caccgtcgtg cccctcaacc aggtggcgct ggtggcggcg
     4801 cccgagccga ggctcctcac ggtccagccg ttcgacaagg ggctcgcctc cgcggtcgaa
     4861 aaggccatcc gcgactcggg gctcggcctc aaccccgcca cgcagggctc catcatccgg
     4921 gtgccgctcc cccagctgac cgaggagcgc cgcaaggagt tggtcaagct ggtgcacaag
     4981 ctcgccgagg aaggccgggt ggcggtgcgg caggcccgcg gcgaggcgca tgccaaggtc
     5041 aagaagctcc agggcctccc ggaggacgac gtcaagcgcg ccgagaagga ggtccagaag
     5101 ctgacggacg acgcggtgcg ccaggtcgac cagctggtgg cggggaagga aaaggagttg
     5161 atggaggtct gacgtgaccc acgacgacct gctcgaccgg ctcaggctcc aagggggcat
     5221 ccccgcccac gtcgccatca tcatggatgg caacgggcgg tgggccaccg gccgctcgct
     5281 gccgcgctcg caggggcact acgcggggat gggcgcggtg cgggaggcgg tcgagggcgc
     5341 gctggcggcc ggggtcgagg tgctcacgct ctttgccttc agccaggaga actggcagcg
     5401 ccccgccgac gagatcgacg ccctgatggc gctcctcgag gagtacatcg ccaaggaggc
     5461 gcaggagctc cggcaggccg gcgtgacggt ccggatgctg ggcgacctcg accggctggt
     5521 cccctcggcg cggcaggcgg tcgaccgcat cgagcgcgac acccagggcg gtgagaagct
     5581 ggcgctcaac atctgcatct cctacggcag ccgggccgag ctcacccgcg ccgcccgact
     5641 cctggccgag caggccgcgc ggggcgagct cgaccccgcc gacatcgacg aggcggcgat
     5701 cgcccgccag ctctacaccg cgccgtggcc cgatcccgac ctcctcatcc gcacctccgg
     5761 cgagatgcgg ctctcgaact tcctcctctg gcagctcgcc tacgccgaga tccacgtgac
     5821 gtcggtgctc tggcccgact tcacccggac ggacctcttc cgggccatcc tcgattatca
     5881 gcggcgggag cggcgcttcg gtcgcgtctc ggtctgacat ggccagcaac atgacccgcc
     5941 gggtggcgtt cgccgccgtg gccatcccgc tggcgggcgc cg
//
LOCUS       DASUAP010000226         7065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_933464, whole genome shotgun sequence.
ACCESSION   DASUAP010000226 DASUAP010000000
VERSION     DASUAP010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933464"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFU45_05830"
     CDS             complement(<1..694)
                     /locus_tag="VFU45_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEU5358614.1"
                     /translation="MHKRLIALAFSGGLDTSWCVPHLADLGWTVHTVFVDTGGTTPED
                     RAAVRAQALAVGAVEHHEVDARAQVFTRFVRFLIQGNVLRGEVYPLSVAAERTQQAIA
                     VVDVARSIGAAAVAHGSTGAGNDQIRFDVAFRVLAPDLEIVTPIREQNLQRADAIAEL
                     ERRGLPVPGQAGAYSINRGLWGTTWGGGWTHDPWAGPPAELLDPPAGAPAPREIVLAW
                     RAGLPVALDGAGA"
     gene            complement(1080..2165)
                     /locus_tag="VFU45_05835"
     CDS             complement(1080..2165)
                     /locus_tag="VFU45_05835"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5358615.1"
                     /translation="MSRSHGVLALLSILPAILSAQAKPVSVAEAQAVLAPFVGTYAPS
                     GMEAPMRDAVRRALPAWARPSVDSAGDLLLSVGRGAPHVVFIAHMDEIGFSVTAIRDD
                     GSLELRPLGGFLPSLFEAEPALVHTGAADVPGVFTPADSQPAGRRGLAFRVDLGVTTR
                     AAAESLGVSVGNSVTMPKSYARLAGTRATGRSFDDRVGAAALVLAVRHLDRSRLRHAV
                     TFVWSVREETGLDGAQVVARSLGTSVARVHAIDTFVSADSPLEPQNFAVAPLGRGPVA
                     RAVDQSSVTPPAYVDSLVALARARRIPLQVGATNGGNDGSAFAPWGVVDVPIGWALRY
                     AHSPVEVADLRDVAGLADLVRAVAEGW"
     gene            complement(2162..3106)
                     /locus_tag="VFU45_05840"
     CDS             complement(2162..3106)
                     /locus_tag="VFU45_05840"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358616.1"
                     /translation="MRWRSTFLVLFIVVCAPAARAQAPAALAVRLSGMTAVTGFERAM
                     GDTLLSLVPGSVRDRAGNVVRVIGAGTGGLLVACPMDEVGYVVGGIRADGWLTLRRVG
                     GGVDPRYDRWLQGERITLWGRRGPRAGVVAVRSVHLLRGRGQGEAPFTVDDALVDVGA
                     SDRLGARALGLGVLTPVAREKRPIQYGNARLAAPWAGRRSACAALVVAAGAAHPQGRV
                     VVAFVTEEQLGGRGLLTVANTLGAFDRTILVDAKAGTLGALIESPDSTLAAREPGVGT
                     ATRLDLPTLYPGTPVETVSLSDVQALADRLVRQMEGAR"
     gene            3213..4229
                     /locus_tag="VFU45_05845"
     CDS             3213..4229
                     /locus_tag="VFU45_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU5358617.1"
                     /translation="MPLWTAAVFSAVIAALLVLLLNRARGPDPALTRELADLSARLDA
                     LTGAQQSVPGTVATLTDTVSRQLAQSNAAVTDLRDRIGRLSDATARLEGVGRQVAEVQ
                     DLLRVPKLRGTLGEVWLEELLRQVFPAGRFSMQHTFRSGERVDAVLRIGDGLVPVDAK
                     FPLEAFQRMLATEGDAADKERKAFRRSIRARIDEIADKYIRPDEGTFEFALMYLPSEA
                     VYYESVVRGEELEDGRSVLGYAMERRVIPVSPHTFYAYLSAVLHGLKGLRVEERSRDI
                     LAELGGLRQQLDRFWAAYDKVGTHLGNAVRQFEETGKERERIGARFERLSRGDDDRPP
                     TLPA"
     gene            complement(4257..4874)
                     /locus_tag="VFU45_05850"
     CDS             complement(4257..4874)
                     /locus_tag="VFU45_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5358618.1"
                     /translation="MSNVATQLTVPEAAEARRSIRKYAAEPIPLDDVREILRLTGLAP
                     TPFNVQPARYVVVTDPDLKGRLAQAAYQQPQVTSAPAVIVVYSDMVDALATLEEVVHP
                     GYPVDQRAGVKAGIERGFSGKPAEENQAWGHAISYTNLGYLLLVARSLGYSTSAMLGF
                     QPDEVKRVLGLPAHVTIPALVAIGKGDEEGFPHHRHPIERVAAFR"
     gene            5154..6638
                     /locus_tag="VFU45_05855"
     CDS             5154..6638
                     /locus_tag="VFU45_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU5358619.1"
                     /translation="MRRSLIVGLGLAVGLAPAASAQAAGSIDIGVFGRGVDYDKSLSN
                     KTGFGVGGRVGIFVANGWALAADLSSNSTTNKVTSASVTTTPIHVRVEKYWGTIDQFV
                     LGAGYAHQKVSPLTSGADGTSDGLSGAVGYEHRFGNVVGLRLDAILDYYPTGANDVAG
                     PPKTTNINYSLQAGLNFRFAAHQGPKDSDHDGVPDKEDVCPGTPAGTAVDAKGCPLPK
                     DSDHDGVTDDHDACPGTPAGIRVDARGCPLPIDSDHDGVPDNADACPGTPAGQKVDAR
                     GCPIPIDSDGDGVTDDKDACPGTPAGQKVDARGCPLPMDSDSDGVTDDKDACPGTPIG
                     QKVDARGCPLPVDSDGDGVSDNLDKCPGTMAGQKVDASGCPLLFEPGKASVTLTGVTF
                     VSGRAVLTPNAKAILDGVAASLQANPTIRVEVGGYTDNAGSRLRNITLSRARANAVAN
                     YLIQKGVSPSRLTARGYGPANPVATNATAAGRAENRRVELKRLP"
     gene            complement(6711..>7065)
                     /locus_tag="VFU45_05860"
     CDS             complement(6711..>7065)
                     /locus_tag="VFU45_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5358620.1"
                     /translation="MATGIQMLTGGVGLLVLFLLAPGAGQFELAAVTGRSILALCYLI
                     VFGSIITYSAFVWLLQVTTPAKLSTYAYVNPVVALGLGWAIAGEALDARSIGASVLIV
                     AAVATITWDQSRQPA"
BASE COUNT         1145 a         2537 c         2460 g          923 t
ORIGIN      
        1 ccgcgccggc gccatcgagc gccaccggga ggccggcacg ccaggccagg acgatctccc
       61 gcggcgcggg cgcccccgcc ggcgggtcga gcagctcagc gggcggcccc gcccacggat
      121 cgtgggtcca gccgccgccc caggtggtgc cccagagccc gcgattgatc gaatacgcgc
      181 ccgcctgccc ggggaccggg agcccccgcc gctcaagctc ggcgatggcg tcggcgcgct
      241 ggaggttctg ctcgcggatg ggggtgacga tctcgaggtc gggcgccagg acccggaagg
      301 ccacgtcgaa acggatctgg tcgttgccgg cgccggtgga cccgtgggcc accgccgccg
      361 cgccgatcga gcgggccacg tccaccaccg cgatggcctg ctgggtccgc tcggccgcca
      421 cgctcagcgg atacacctcc ccccggagca cgttgccctg gatgaggaag cgcacgaacc
      481 gcgtgaacac ctgcgcccgc gcatccacct cgtggtgctc caccgcgccc accgcgagcg
      541 cctgtgcccg caccgccgcg cgatcctccg gcgtggtgcc gccggtgtcc acgaagacgg
      601 tgtgcaccgt ccagccaagg tccgcgagat ggggaacaca ccacgacgtg tcgagcccgc
      661 cggagaaggc gagggcgatg aggcgcttat gcatagtgca taaatatgca atatgcacgg
      721 caggcgcaag aggggcggca agatgtggga ccacggagta cgcggagcac acggagagac
      781 acggagaaaa gcaggtgatg tggtctcgcg agaggaactc cgtgtccgca tcggagtcat
      841 cccgagcgga gcgagggacc ttgccagccg ggccccgcac ccggtggccg cactccgatg
      901 atccccgccg aagatccctc ccctcgctac gctcggtctc gggatgactc ggaagtgcga
      961 tcggaatccc gcccgcgagc ccacgtcacc agcagtcctc cgtgcctcgg agcctgtccg
     1021 gggcgaagcg aaggaagtgc tccgtgccct ccgtggtccc actcccagct ggcgccccgc
     1081 taccatccct ccgcgacggc ccggaccagg tccgcgaggc ccgccacgtc ccggagatcg
     1141 gccacttcca ccggcgagtg ggcgtagcgg agcgcccagc cgatcgggac atcgaccacg
     1201 ccccacggcg cgaaggccga tccgtcgttc ccgccgttcg tggccccgac ctggagcggg
     1261 atgcggcggg cccgggccag cgccacgagc gagtcgacat acgccggcgg cgtcaccgag
     1321 ctctggtcca cggcccgcgc caccggccct cggcccagcg gcgccacggc gaagttctgc
     1381 ggctcgagcg gcgagtcggc ggagacgaac gtgtcgatgg cgtgcacccg cgccaccgat
     1441 gtgcccagcg accgggcgac cacctgcgcg ccgtcgagcc cggtctcctc ccgcacgctc
     1501 cacacgaacg tgacggcgtg ccggagccgc gaccgatcga ggtggcggac cgcaaggacc
     1561 agcgccgccg cccccacccg gtcgtcgaag gagcgcccgg tggcccgggt ccccgccagg
     1621 cgcgcgtagg acttgggcat cgtgacgctg ttgccgacgc tgacaccgag cgattccgcg
     1681 gccgcacggg tcgtgacgcc caggtccacc cggaacgcga gccccctccg gcctgcgggc
     1741 tgtgagtcgg cgggcgtgaa cacacccggc acgtcggcgg ccccggtgtg gaccagggcc
     1801 ggctcggcct cgaacaggga cggcaggaac ccgcccagcg gccgaagttc gagcgagccg
     1861 tcatcgcgga tggccgtgac gctgaacccg atctcatcca tatgggcgat gaacacgacg
     1921 tgaggcgcgc cccgccccac ggagaggagc agatccccgg ccgagtcgac ggacggacgg
     1981 gcccaggcgg gtagcgcccg ccgcacggca tcgcgcatcg gggcttccat cccggagggc
     2041 gcgtacgtcc cgacgaacgg cgccagcacc gcctgggcct cggccaccga cacgggcttg
     2101 gcctgcgccg acaggatcgc gggcaggatg gacagcaggg cgaggactcc gtgactccgg
     2161 ctcatcgggc accttccatc tggcggacca gccggtcagc caacgcctgg acatcggaaa
     2221 gcgagacggt ctcgaccgga gtgccgggat acagggtggg cagatcgagg cgcgtggcgg
     2281 tgccgacgcc cggttccctg gcagcgagtg tggagtcggg gctctcgatg agtgcgccga
     2341 gggtgcccgc cttcgcatcc acaaggatgg tccggtcgaa cgcccccagg gtgttggcga
     2401 cggtcaggag cccgcggccg cccagctgct cttccgtgac gaacgccacg acgacccgcc
     2461 cctgcgggtg cgccgcgcct gccgcgacca cgagtgccgc gcaggcggag cgccgaccgg
     2521 cccacggcgc cgccagccgg gcgttcccgt actggatcgg gcgcttctcc cgcgccaccg
     2581 gcgtcaggac gccgagcccc agggccctcg cgccgagtcg gtcgctcgcg ccgacgtcca
     2641 ccagtgcatc gtccacggtg aagggcgcct cgccctggcc gcgtccccgg agaaggtgga
     2701 cggaccgcac cgcgacgacc ccggcccgcg gaccgcgccg gccccagagc gtgatgcgct
     2761 ccccctggag ccaccggtcg taccgggggt ccactccgcc accgacacgc cgcagcgtga
     2821 gccatccgtc ggcgcggatc ccgccgacca cataccccac ctcatccatg gggcacgcca
     2881 ccaggagccc gccggtgccg gcaccgatca cccgcaccac gttcccggcg cgatcgcgga
     2941 ccgaaccggg aacgagggac aggagcgtgt ctcccatcgc gcgttcgaag ccggtcaccg
     3001 ccgtcatccc ggaaaggcgg accgccagtg ctgccggagc ctgtgcgcgg gcggcaggcg
     3061 cgcacacgac tatgaacagg acgaggaagg tcgaacgcca gcgcatcgca ccgccaggaa
     3121 ggaggggagt tccatccaat ctacgcatcg gcgccgacgc tgcttgccac acctgggcga
     3181 tactattcag ctccccatcg ccggagtcgc cgatgcccct ctggaccgcc gcggtgttca
     3241 gtgccgtgat cgcggcactc ctggtcctcc tgctgaaccg cgcccgcggt ccggacccgg
     3301 cgctcacccg ggagctggcc gacctcagcg cccgcctcga cgcgctgacc ggtgcccagc
     3361 agtccgtgcc cggcaccgtc gccacgctca ccgacaccgt gtcgcgccag ctcgcacagt
     3421 ccaacgccgc ggtcaccgac ctgcgcgacc ggatcggccg gctgtccgac gccacggccc
     3481 ggctcgaagg cgtggggcgc caggtggcgg aagtacagga cctgctccgg gtccccaagc
     3541 ttcgcggcac gctcggcgag gtgtggctgg aggagctgct gcgtcaggtg ttccccgccg
     3601 gccgcttctc gatgcagcac acctttcgct ccggcgagcg ggtggacgcc gtactccgca
     3661 tcggggacgg cctggtgccg gtggacgcca agttccccct tgaggcgttc cagcggatgc
     3721 tcgccaccga gggcgacgcg gcggacaagg agcggaaggc gttccgccgc tccatccgcg
     3781 cccgcatcga cgagatcgcc gacaagtaca tccgccccga cgaggggaca ttcgagttcg
     3841 cgctgatgta cctcccgtcg gaggcggtgt attacgagag tgtggtgcgg ggggaagaac
     3901 tcgaggacgg acggagcgtg ctcggctacg cgatggagcg ccgggtgatc ccggtctcac
     3961 cccacacgtt ctacgcctac ctctccgcgg tcctgcacgg cctcaaggga ctccgggtcg
     4021 aggagcgctc gcgcgacatc ctggcggagt tgggcggcct gcgccagcaa ctcgaccggt
     4081 tctgggccgc gtacgacaag gtgggcaccc acctcggcaa cgcggtccgc cagttcgagg
     4141 agaccggcaa ggaacgggag cggatcggcg cccggttcga gcggctgagc cgcggcgacg
     4201 acgaccgacc gcccacactg cccgcctgac gactgaccgc gggccggggt gcaagatcag
     4261 cggaacgcgg cgacgcgctc gatcgggtgg cggtggtgag ggaacccctc ctcgtcgccc
     4321 ttgccgatag ccaccagggc cgggatggtg acgtgggccg gaaggcccag tacccgcttc
     4381 acctcgtcgg gctggaagcc gagcatcgcg gaggtgctgt acccgaggga ccgggccacc
     4441 aggagcaggt agccgaggtt ggtgtagctg atggcgtggc cccaggcctg gttctcctcg
     4501 gcgggcttcc ccgaaaagcc gcgctcgatc cccgccttca caccggcgcg ctgatccacc
     4561 ggatacccgg gatgcaccac ctcttcgagc gtggcgagcg cgtcgaccat gtcgctgtag
     4621 accacgatga cggcgggtgc ggacgtcacc tgcggctgct gatatgcggc ctgcgccagc
     4681 ctgcctttga ggtcgggatc ggtcaccacg acgtatcggg ccggctgcac gttgaagggg
     4741 gtgggcgcca ggcccgtcag ccgcaggatc tcgcggacgt cgtcgagcgg gatcggctcg
     4801 gcggcatact tgcggatcga ccgacgtgct tcggcggcct ccggcacggt caattgggtg
     4861 gcgacgttgc tcatattccc cctcaaggtt cgtgaccgac gacaggttca cgcccccaag
     4921 ttatctgtta taacgactat tgtcaatacg gagattgtcg agtggagtgt tcgccgttgc
     4981 accgaaattg tgcaacattg tgacgtggct caccgaggcc acaagatcga agccgatatc
     5041 ctccgttcac tactatagat attcacgccg gattcacccc ggcctgacgc ggcgattgct
     5101 agcctgaccc atctcggtcg gacatccgat ccgagccacc tgaggagctc cccatgcgca
     5161 ggtcactcat cgtcggtctc ggcctggccg tggggctggc cccggccgca tcggcccagg
     5221 cggccggcag catcgacatc ggcgtgttcg gccgcggcgt ggactatgac aagagcctga
     5281 gcaacaagac cggcttcggc gtgggaggcc gcgtcgggat cttcgtggcc aacggctggg
     5341 cgcttgcggc tgacctgtcg tccaattcga ccacgaacaa ggtgaccagc gccagcgtca
     5401 ccacgacgcc gatccacgtc cgggtggaga agtactgggg cacgatcgac caattcgtgc
     5461 tcggcgccgg atacgcccac cagaaggtga gtccgctcac cagcggagcc gatggcacgt
     5521 ccgacgggct ctcaggcgcc gtcggctatg agcaccggtt cgggaacgtg gtcgggctcc
     5581 ggctcgacgc gatcctcgac tactacccca ccggtgcgaa cgacgtggcg gggccgccca
     5641 agacgaccaa tatcaactat tcgctgcagg cggggctcaa cttccgcttc gcagcgcacc
     5701 agggaccgaa ggacagcgac cacgacggcg tgcccgacaa ggaggatgtc tgcccgggga
     5761 cgcctgccgg gaccgcggtc gacgcgaagg gctgccccct gcccaaggac agcgaccacg
     5821 atggcgtcac cgatgaccac gacgcctgcc cgggcacgcc ggccgggatc cgggtcgatg
     5881 cgcgcgggtg cccgctcccg atcgactccg accacgatgg cgtgccggac aacgccgatg
     5941 cctgtcctgg cactcccgcc ggccagaagg tcgacgcacg cgggtgcccc atcccgatcg
     6001 actctgacgg cgatggcgtg accgacgaca aggacgcctg ccccggcacg cccgccggcc
     6061 agaaggtgga tgcccgcggc tgtccgttgc cgatggactc ggatagcgac ggcgtgaccg
     6121 acgacaagga cgcctgcccc ggcacgccga tcggccagaa ggtcgatgcg cgtggctgcc
     6181 cgctgccggt cgattccgac ggcgatgggg tctcggacaa cctcgacaag tgccctggca
     6241 ccatggcggg ccagaaggtc gacgcgagcg gctgcccgtt gctcttcgag cccgggaagg
     6301 cgagcgtgac actgacgggg gtcaccttcg tgagcggacg ggcagtgctg acgcccaacg
     6361 cgaaagccat cctcgacggc gtggccgcct cactccaggc caacccgacg atccgggtgg
     6421 aggtcggcgg gtacaccgac aatgcgggat cgcgcctgcg caacatcacg ctctcgcgcg
     6481 cccgggccaa cgccgtggcc aattacctga tccagaaagg cgtctcgccc tcgcggctga
     6541 ccgcgcgcgg ctacggtccc gccaacccgg tggcgaccaa tgccaccgcc gcgggacggg
     6601 cggagaaccg gcgggtggag ctcaagcgat tgccgtgatg ccggcgtgac cggcaggtgg
     6661 gaagaatggg cgcgggggct ccaggcggag cccccgcgtt ccgttgtggt tcaggcaggc
     6721 tggcgggact ggtcccaggt gatggtggcg accgccgcca cgatgagcac cgaggcaccg
     6781 atgctccggg cgtcgagcgc ctcgccggcg atggcccagc cgagtccgag cgccaccacg
     6841 gggttgacat aggcgtaggt ggagagcttg gccggcgtgg tgacctgcag cagccagacg
     6901 aaggcgctgt aggtgatgat cgacccgaac acgatgaggt agcagagcgc caggatcgaa
     6961 cggccggtga ccgccgccag ctcgaactgc cccgccccgg gtgccaggag gaagagcacg
     7021 agcaggccga cgccgccggt aagcatctgg atgccggtgg ccatc
//
LOCUS       DASUAP010000227         3723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_579058, whole genome shotgun sequence.
ACCESSION   DASUAP010000227 DASUAP010000000
VERSION     DASUAP010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579058"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFU45_05865"
     CDS             complement(<1..488)
                     /locus_tag="VFU45_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358621.1"
                     /translation="MRPAFLAVLGSLVAAPAAAQAITPPPDAPATVELRDTLLILRYH
                     GAKIFEGTIVTAGGPASFRELTDTTDGRVTQVLKWTAPDDGRITITGLVRGSPEAFAA
                     EAEPHHDALPVVRHAVGAVADRLDRAFYDRRWDWVLSVDDPAGAELVPAVDPDSGVSY
                     RLT"
     gene            586..3225
                     /locus_tag="VFU45_05870"
     CDS             586..3225
                     /locus_tag="VFU45_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5358622.1"
                     /translation="MTPPVERLAAALADRYRLERELGAGGMATVYLAHDLKHDRKVAL
                     KVLRPELAAVIGAERFVVEIRTTAALQHPHILPLFDSGTADGFLFYVMPYIEGETLRD
                     KLNRETQLAIDDAVRITTDVADALDYAHRHGVIHRDIKPENILLHDGRPVVADFGIAL
                     ALSAAAGGRMTETGMSLGTPHYMSPEQATAEKEITGRSDIYSLGSVLYEMLTGQPPHL
                     GGSAQQIIMKIIAEPVRPVTALRRSVPPNVAEAVAKAVEKLPADRFDSAKAFATALHD
                     PGFRAGVAADGARTAVPVGLGWRDWRAWSAAAVLVAAAALLGRWSAPVRRAAPVTARF
                     SIAVDSGRYITNGPVNALALSPNGQTIVYVGRTATSTQVQLYTRPFDGTASHPVSGTE
                     GASYPAFSPDGRRLAYIGPGGVYAIDVPLVGGTSAPIVGSVVQALSGMTWLNDNTLVA
                     SSDAGDLITISLSGTSTIAARPDRAVGEKNLYPEAALPDGRTMLVLPSHGSGIVGPVV
                     AVDTRSGRRTPILDGPANAVWYADGALLWGEPNGAIRGALFDPGKLKVLSAPVTIADS
                     VQQEIGGGPQAGVSANGSLVYVPESPLSLVLADHAGKERVLAEGHRFHSPRFSPDGSR
                     LALDFTQQGSRSVWILDLGQLTLRRLSFDNDGHDPVWSRDGKWVAYEHNGGIWRRRSD
                     GSGDADSVYTGPGAEQVLEYAPDGSMLGASLGTRATFGVVHLSADTRKIEPLVSTDYS
                     VQEGTLSPDGRWMAYLSDETGRYEVYVQPFPGGAKTLVSQGGGIEVRWGPDGRTLYYD
                     GLENGRPTLIEATVRTAPDFGVLKRTPLFDASDFEPAEPHANWDISPDGARFVMAHQA
                     PFNTLAVVLGWTEEVRRHSAATR"
     gene            3225..>3723
                     /locus_tag="VFU45_05875"
     CDS             3225..>3723
                     /locus_tag="VFU45_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEU5358623.1"
                     /translation="MTTPTPRKPDDDEIDVYGLSHAGKVRPDNQDHFLLASLHKRLTI
                     HQTNLTAAERLPFGDRRLAYIAMVADGVGGGAGGAEASATALETATQYILGSMDCYDR
                     AGAGSDEGTFIDALQDAAMRAHQAVVARAQAGPERGRMATTLTLWMGVWPWYYLLQVG
                     DSRYYL"
BASE COUNT          559 a         1368 c         1294 g          502 t
ORIGIN      
        1 gtgaggcgat aggaaacgcc gctgtccgga tcgaccgccg ggacgagctc ggcgccggcc
       61 ggatcatcca cggacagcac ccagtcccag cgccggtcgt agaaggcccg gtcgaggcgg
      121 tcggccacgg cgcccaccgc atggcgcacc accggaaggg catcgtggtg cggctccgcc
      181 tccgccgcga acgcctcggg agatccacgg accaggccgg tgatggtgat ccggccatcg
      241 tcgggcgcgg tccacttgag gacctgcgtc acccggccat cagtggtgtc ggtgagctcc
      301 cggaacgatg ccgggccccc cgccgtcacg atcgtcccct cgaagatctt cgccccgtgg
      361 tagcgcagga tcaggagggt gtcccgcagc tcgaccgtgg cgggcgcgtc ggggggcggg
      421 gtgatggcct gcgcggcggc gggcgcggca acgagggatc cgagaacggc gaggaaggca
      481 ggtctcatag cacggtaggt tgggcgcccg ccgcgggaat ggcaagcgga cgcccgacgg
      541 agccaccctc gcggaagtcc ccggagacca gcatctttcc cctgcgtgac accccccgtc
      601 gaacgcctcg ctgccgccct ggccgaccgc taccgtctcg agcgggaact cggcgcgggc
      661 gggatggcca cggtctacct ggcccacgac ctcaagcacg accggaaggt cgccctcaag
      721 gtcctgcggc cggagcttgc ggccgtcatc ggcgccgagc gcttcgtggt cgagatccgg
      781 accaccgccg cgctccagca cccgcatatc ctcccgctct tcgattccgg caccgccgat
      841 ggcttcctct tctatgtgat gccgtacatc gagggtgaga cgctccgcga caagctgaac
      901 cgcgagaccc aactcgccat cgacgacgcg gtgcggatca cgaccgacgt cgccgatgcc
      961 ctcgactatg cccaccgcca cggcgtcatc caccgcgaca tcaagccgga gaacatcctc
     1021 ctccacgacg gccgcccggt cgtggccgac ttcgggatcg cgctggcgct ctccgccgcg
     1081 gccggcgggc ggatgaccga gaccggtatg tcgctcggca ccccgcacta catgagcccc
     1141 gagcaggcca ccgccgaaaa ggagattacc ggccggagcg atatctactc gctgggcagc
     1201 gtgctgtacg agatgctgac cggccagccg ccccacctcg ggggctcggc ccagcagatc
     1261 atcatgaaga tcatcgccga gccggtccgg ccggtcaccg cgctgcggcg gtcggtgccg
     1321 cctaacgtgg cagaggcggt ggccaaggcg gtggagaagc tcccggccga ccggttcgat
     1381 tccgccaagg ccttcgccac ggcacttcac gacccgggct tccgcgctgg cgtggcggcc
     1441 gatggcgccc ggaccgcggt gccggtgggc ctggggtggc gcgactggcg cgcgtggtcg
     1501 gcggcggcgg tactcgtcgc ggcggcggcc ctcctcgggc gctggtcggc gccggtgcgt
     1561 cgggcggcgc cggtcacggc ccgcttcagc atcgcggtcg actccgggcg ctacatcacc
     1621 aacggcccgg tcaacgcgct cgccctctcg cccaacgggc agaccatcgt gtatgtcggc
     1681 cgcacggcga cgagcaccca ggtccagctc tacacccgcc cgttcgacgg gaccgcgtcc
     1741 cacccggtct ccggtacgga gggggcctcc tacccggcat tttctccgga tggccgccgg
     1801 ctggcgtaca tcggtcccgg cggtgtgtac gccatcgacg tacccctcgt cggtggcact
     1861 tcggcgccga tcgtgggcag cgtggtccag gcgctcagcg gcatgacctg gctcaacgac
     1921 aacacgctcg tcgcctccag cgacgcgggc gacctcatca ccatctcgct cagcggcacg
     1981 tccaccatcg ccgccaggcc ggaccgggcg gtcggcgaga agaacctcta ccccgaggcg
     2041 gcccttcccg atgggcggac catgctggtc ctcccctcgc acgggtccgg gatcgtcggg
     2101 ccggtcgtgg cggtggacac acggagcggc cgccgcaccc cgatcctcga cgggcccgcg
     2161 aacgccgtct ggtacgccga cggggccctc ctctggggcg agccgaatgg ggccattcgc
     2221 ggcgcgctct tcgaccccgg caagctcaag gtcctgagcg cgcccgtcac catcgccgac
     2281 tcggtgcagc aggagatcgg cggcggcccc caggcgggcg tctcggccaa cggctcactg
     2341 gtctacgtgc cggagtcgcc actctccctc gtgctggcgg accatgccgg aaaggagcgg
     2401 gtgctggcgg aggggcatcg gttccacagc ccccgcttct cgccggacgg gagccgcctg
     2461 gcgctcgact tcacccagca ggggagcagg agcgtctgga tcctcgacct cgggcagctg
     2521 accctgcgcc gcctgtcgtt cgacaacgac gggcacgatc cggtctggtc gcgcgacggg
     2581 aagtgggtgg cctacgagca caacggcggg atctggcggc gccggagcga cggcagtggc
     2641 gatgccgact cggtctacac cgggcccggc gccgagcagg tgctcgagta cgcgcccgac
     2701 gggagcatgc tgggcgcctc gctcgggacg cgggccacgt tcggcgtggt gcacctgtcg
     2761 gccgacacca ggaagatcga accgctcgtc tcgaccgact acagcgtgca ggaaggcacg
     2821 ctctcacccg atggccggtg gatggcgtac ctgagcgacg agaccggtcg ctacgaagtg
     2881 tacgtgcagc ccttcccggg cggcgccaag accctcgtgt cgcagggagg cgggatcgag
     2941 gtccgctggg ggccggacgg gcggacgctc tactacgacg gcctggagaa cgggaggccg
     3001 acgttgatcg aggcgacggt gcggaccgcg ccagacttcg gcgtcctgaa gcgcacgccg
     3061 ctcttcgacg cctccgactt cgagcccgcc gagccgcacg ccaactggga catctcaccc
     3121 gacggggccc gcttcgtgat ggcccaccag gccccgttca acacgctcgc cgtcgtgctc
     3181 ggctggaccg aggaggtccg ccgccactcg gcggccacgc gctgatgacc acgcccactc
     3241 cgcggaagcc cgacgatgac gagatcgacg tctacggcct gtcgcacgcc ggcaaggtgc
     3301 gccccgacaa ccaggaccac ttcctcctgg cctcgctcca caagcgcctc accatccacc
     3361 agaccaacct gaccgcggcc gagcgcctgc ccttcggcga ccggcggctg gcgtacatcg
     3421 cgatggtggc cgacggcgtc ggcggcggcg ccggcggcgc ggaggcgagc gccaccgcgc
     3481 tggagaccgc cacccagtac atcctgggaa gcatggactg ctacgaccgc gctggagcgg
     3541 gaagcgacga gggcacgttc atcgatgccc tgcaggatgc cgcgatgcgg gcgcaccagg
     3601 cggtggtggc acgcgcccag gccggcccgg agcgcggacg gatggccacc accctcacgc
     3661 tgtggatggg cgtgtggccc tggtactacc tgctccaggt gggcgacagc cgctactacc
     3721 tct
//
LOCUS       DASUAP010000228         4587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_605944, whole genome shotgun sequence.
ACCESSION   DASUAP010000228 DASUAP010000000
VERSION     DASUAP010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605944"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="VFU45_05880"
     CDS             complement(<1..1712)
                     /locus_tag="VFU45_05880"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5358624.1"
                     /translation="MPATVELPVSGMTCAACSARVQRALEQAPGVAAANVNLMTNAAT
                     VTFDPVATSPDALVETIRATGYGAELPSPDTGALDQVSRQDEANLAERRELRQKLTVS
                     AVAAVLTMLLSMPLARLEMTNGPVDPLMRWMMPLGDWLLQAIPGLAALSADALRLVLL
                     GLTLPVVFWAGRHFYTRAWSAFRHHGADMNTLIAVGTGAAFLWSLTATAGAHWLRARG
                     LAPDVYYEAVVWIVALILLGNLLDARARGRTADAIRALVKLRPQTAHRVSDGIEEDVP
                     LERIVVGNDLQVRPGEHIPTDGVVLDGTSDVDESMLTGEPMPVAKSAGHEVTGGTVNG
                     AGAFRMRATRVGRDTVLAHIVRLVREAQGQKAPIQQLADRISAIFVPVVLGIAVATFA
                     VWYVVGPEPRSLQALVSAVTVLIIACPCAMGLAVPTAVMVSTGRGAQLGVLIKGGESL
                     QRASAIDVVLVDKTGTVTEGHPAVTAVHPLTGNEAQLLRLAAAVERLSEHPLARAIVS
                     AAKSRQLSPDLAADFKAVPGQGAAGSVDGRVVLVGNAEWMRASGVSIGAAEPIAERLA
                     GEGAT"
     gene            complement(1796..2188)
                     /locus_tag="VFU45_05885"
     CDS             complement(1796..2188)
                     /locus_tag="VFU45_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5358625.1"
                     /translation="MSADRVLVLVLAAAAIAWIWWYFFAVPARAAGVAAALKGGIQEQ
                     AIAVATAYDPSVVRVRAGAPVRLTFTRTDTNSCTEEVVLGDFNIRTFLPTGTPTAVEF
                     TPRTPGRYDFHCGMGMVHGTLIVERREG"
     gene            complement(2185..2394)
                     /locus_tag="VFU45_05890"
     CDS             complement(2185..2394)
                     /locus_tag="VFU45_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU5358626.1"
                     /translation="MTPLSLSITGMSCGHCLNAVNQALGKLDGVKLGSVKMGRAELEY
                     DESKLTPAAVTAAVSAVGYQASVIA"
     gene            complement(2621..4222)
                     /locus_tag="VFU45_05895"
     CDS             complement(2621..4222)
                     /locus_tag="VFU45_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5358627.1"
                     /translation="MPETEQGPANRRRTIAARLSRMPLAIAGAIIAGWVGCLLAYYQH
                     IAGVAVMALATALAFYARRRAQEIVDAEVEGIARALDAAAARNRELESFRHLSQVLLS
                     GRPLPELFHEIARLAAELLEGEASGIGVVTEEGRFLRIVAGTGLMAHAVDRLVPVDGS
                     LAGWVVGHDRPLIADDMRQDPRNFDFPDTPQALTSAVMTPLRSSGLVIGTVAVMNRRD
                     GKFFHEDDLYLLQALADQAALGLDRANVFEESQRNALALAEKNRELEHATRLKDEFLA
                     NVSHELRTPLNAIIGFSELLLTGDVGEVAEAHREFLESIHRNGKHLLRLINGVLDMSK
                     MDAGRMSLELTRTDLRDAIRAAAVDTESLRLGRKQVHRFELPDTPLFALADAVRVQQI
                     LFNLLSNASKFTPEGGEIVLRALATEAPLPIPAERSDDQVKRISRPAVWIAVSDNGIG
                     IKPDDMSKLFVEFSQVDSSPSRRVQGTGLGLALCKRFVELHGGQIGAESMYNFGSTFW
                     FILPAEGPLRRWGARTSDETAAVAS"
     gene            complement(4300..4497)
                     /locus_tag="VFU45_05900"
     CDS             complement(4300..4497)
                     /locus_tag="VFU45_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358628.1"
                     /translation="MLTIDRCVCRNTLFAELLPRARAEGWDLTQLVAVTGCGGQCGLC
                     RPYLKRMLATGETVFTEILLD"
BASE COUNT          743 a         1436 c         1736 g          672 t
ORIGIN      
        1 gtggcgccct caccggccaa ccgctcagcg atgggctcgg cggcgccgat gctcacaccc
       61 gaggcgcgca tccactccgc gttccccacc agcaccaccc gtccgtccac tgagccagcg
      121 gcgccctggc cggggacggc cttgaagtcg gcggcgagat cgggcgagag ctgccgtgac
      181 ttggcggccg aaacgatggc gcgggccagc ggatgctcac tcagccgctc gaccgccgcg
      241 gcgagccgga ggagctgggc ctcgttgccg gtcagcggat gcaccgcggt cacggcgggg
      301 tggccttcgg tgacggtccc ggtcttgtcg accagcacca cgtcgatggc gctggcccgc
      361 tgcagcgatt cacccccctt gatgagcacg ccgagctgcg cgccgcggcc ggtggagacc
      421 atcaccgccg tcgggaccgc gagccccatg gcacaggggc aggcgatgat gagcaccgtg
      481 accgccgaga cgagcgcctg gagcgagcgg ggctcggggc cgaccacgta ccagaccgcg
      541 aacgtggcga cggcgatgcc gaggacgacc ggcacgaaga tggcggagat ccggtcggcg
      601 agctgctgga tcggggcctt ctggccctgc gcctcgcgga ccaggcggac gatgtgggcg
      661 agcaccgtgt cgcgccccac cctggtggcc cgcatccgga aggcgccggc gccgttgacg
      721 gtgccgccgg tgacctcgtg gccggcgctc ttggcgacgg gcatcggctc gccggtgagc
      781 atcgactcgt cgacgtcgga ggtgccatcc agtacgacgc cgtcggtggg aatgtgctcg
      841 ccggggcgga cctggagatc gttgcccacg acgatccgct cgagcggcac gtcctcttcg
      901 atgccgtcgc tgacgcgatg ggcggtctgc ggacgcagct tgaccagcgc gcggatggcg
      961 tcggcggtgc ggccgcgggc ccgggcatcg aggaggttgc cgaggaggat gagggcgacg
     1021 atccagacca ccgcttcgta gtacacgtcg ggcgccaggc ctcgggcccg gagccagtgg
     1081 gcccccgccg tggcggtgag gctccagagg aaggccgcac cggtgccgac ggcgatcagg
     1141 gtgttcatgt cggcgccgtg gtgccggaac gccgaccagg cgcgggtgta gaagtggcgg
     1201 ccagcccaga aaacgaccgg gagggtgagg cccaggagga cgaggcggag cgcgtcggcg
     1261 gagagcgccg cgaggccggg gatcgcctgc aggagccaat cgcccaacgg catcatccag
     1321 cgcatcagtg ggtcgaccgg gccattcgtc atctcgagcc gggcgagcgg catcgagagg
     1381 agcatcgtga ggactgcggc gacggcgctg acagtgagct tctgccggag ctcccgccgt
     1441 tcggcgaggt tggcctcgtc ctggcgcgac acctggtcca gcgccccggt gtcggggctc
     1501 ggcaactcgg cgccgtagcc ggtggcgcgg atcgtctcga ccaatgcgtc ggggctcgtg
     1561 gccacggggt cgaaggtgac cgtggcggcg ttggtcatca ggttgacgtt cgcggccgcg
     1621 acgccggggg cctgttccag cgcgcgctgg acgcgggcgc tgcaggcggc gcaggtcatt
     1681 ccgctgacgg ggagctcgac ggttgcgggc atgtcagtcg gtgaggggtg agcggtgagg
     1741 ggtgagcggg ctcccattcg aaatctcctt ggaaggcccg ctcactcctg accgcttacc
     1801 cctcacgcct ctccactata agcgtcccat gcaccatccc catcccgcag tggaagtcgt
     1861 agcggccggg agtgcggggt gtgaactcga ccgcggtggg agtgccggtc gggaggaagg
     1921 tgcggatgtt gaagtcaccg agcaccactt cctcggtgca gctgttggtg tcggtgcggg
     1981 tgaacgtgag ccggaccggc gcgccggccc gcacccggac caccgacggg tcgtaggccg
     2041 tggcgacggc gatggcctgt tcctggatcc cgcccttgag cgcggccgcg acgccggcgg
     2101 cccgcgccgg cacggcaaag aagtaccacc agatccacgc gatcgcggcg gcggcgagaa
     2161 ccagcaccag gacccgatcg gcgctcatgc gatgaccgac gcctgatagc cgaccgcgga
     2221 caccgcggcc gtgaccgcag cgggcgtgag cttcgattcg tcgtactcga gttcagcgcg
     2281 gcccatcttg accgagccga gcttgacgcc gtcgagcttg ccgagggcct ggttgaccgc
     2341 gttcaggcag tggccgcagg acataccggt gatggagagg gagagggggg tcatgggtgg
     2401 tcggtccttg gtcgatggtc gttagtcgat ggttgttagt cgttggtcgt tggtggttgg
     2461 tggttggtgg tcagtagcca gtcgctggtg agggaccaac gtccaacgtc caatgaccaa
     2521 tgaccaatga ccaacgacga acgaccaacg accagtagcc acaatatata ggggtagggg
     2581 gtatgtcaat cccctgcgaa acaacgactt ctggagggct tcagctggct acggcggcgg
     2641 tctcgtcgct ggtgcgggcg ccccagcggc ggagggggcc ctcggcgggg aggatgaacc
     2701 agaaggtgct gccgaagttg tacatcgact cggcgccgat ctggccgccg tggagctcga
     2761 cgaagcgctt gcagagggcc aggccgagcc cggtgccctg cacccggcgg ctgggggagc
     2821 tgtcgacctg gctgaactcg acgaagagct tcgacatatc gtcgggcttg atgccgatgc
     2881 cgttgtcgct cacggcgatc cagacggcgg gccgactgat ccgcttgacc tggtcgtcgg
     2941 aacgttccgc agggatgggg agcggcgcct cggtggcgag ggcgcggagg acgatctccc
     3001 caccttcggg cgtgaacttg gatgcgttcg agaggaggtt gaagaggatc tgctggaccc
     3061 ggaccgcgtc ggcgagggcg aagagcggcg tatcggggag ctcgaagcgg tggacctgct
     3121 tccggccgag gcggaggctc tcggtgtcga ccgcggcggc gcggatggcg tcgcgcaggt
     3181 cggtgcgggt gagctcgagg ctcatccgtc cggcgtccat cttcgacatg tcgagcacgc
     3241 cgttgatgag ccggagcagg tgcttgccgt tgcggtggat cgactcgagg aactcgcggt
     3301 gggcctcggc cacctcgccg acatcgccgg tgaggaggag ctcggagaat ccgatgatgg
     3361 cgttgagcgg cgtccggagc tcgtgggaga cgttggcgag gaactcgtcc ttgagcctgg
     3421 tggcgtgctc cagctcgcgg ttcttctcgg ccagggccag ggcgttccgc tggctttcct
     3481 cgaagacgtt ggcgcggtcg aggccgagcg ccgcctggtc ggccagggcc tggagcaggt
     3541 agagatcgtc ctcgtggaag aacttgccgt cgcgccggtt catcaccgcg accgtcccga
     3601 tgacgaggcc gctcgagcgg agcggggtca tgaccgcgga ggtgagcgcc tgcggcgtat
     3661 cgggaaagtc gaagttgcgg gggtcctgcc gcatgtcgtc agcaatgagg gggcggtcgt
     3721 gacccaccac ccacccggcg agcgacccgt cgaccggcac cagccgatcc accgcatgcg
     3781 ccatcaggcc ggtgccggcg acgatgcgga ggaaccgtcc ctcttccgtg accacgccga
     3841 tcccggaggc ctcgccttcc agcagctcgg cggcgagccg cgcgatctca tggaagagct
     3901 ccggcagcgg acgccccgag agcagcacct gggagaggtg acggaaggac tcgagttccc
     3961 ggttccgcgc ggcggccgca tcgagggcgc gggcgatgcc ctccacctcg gcgtcgacga
     4021 tctcctgtgc ccggcgccgg gcgtagaatg caagcgccgt cgccagggcc atcacggcca
     4081 cgccggcgat gtgttgatag tacgccagca ggcagccgac ccagcccgca atgatggcgc
     4141 cggcgatcgc gagcggcata cggctcagcc gcgcggcgat ggtgcgccga cgattggccg
     4201 gtccctgctc ggtctcaggc atcagggaaa tctggcgtca aaacggggcc acgggagggg
     4261 ggagcgtgat gcggacgacg ctgcgccagc ctgcgggcct cagtcaagca ggatctccgt
     4321 gaagaccgtc tcaccggtgg caagcatccg cttcaagtag gggcgacaga gcccgcactg
     4381 cccgccgcag ccggtgaccg ccaccagttg cgtgagatcc cacccttcgg cgcgagcccg
     4441 gggcagcagc tcggcgaaaa gagtgttccg gcagacgcag cggtcgatgg tgagcatgtt
     4501 ctaagtcaaa agtgaaaagg gaaaaggaga gggggacgag ggaaaaggag aaggagaagg
     4561 agaagggaag aagggagatg aggatag
//
LOCUS       DASUAP010000229         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_347120, whole genome shotgun sequence.
ACCESSION   DASUAP010000229 DASUAP010000000
VERSION     DASUAP010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347120"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFU45_05905"
     CDS             complement(<1..306)
                     /locus_tag="VFU45_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358629.1"
                     /translation="MSDDARRRIGAVASLVLGLSLGIALLPIPGAGTLPATVGSILWK
                     ALGAGAIGFPLVGLGVAAAGFGWFERLDMKRTALLVGGLAVLIPLFIGTLLDLGPALF
                     "
     gene            complement(326..1684)
                     /gene="accC"
                     /locus_tag="VFU45_05910"
     CDS             complement(326..1684)
                     /gene="accC"
                     /locus_tag="VFU45_05910"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU5358630.1"
                     /translation="MFKKVLIANRGEIALRVIRACRELGVETVAVYSEADRESLHVRF
                     ADDDVCIGPPPSRQSYLRIPSLIAAAEITGADAIHPGYGFLAENPEFSDTCKASNITF
                     IGPSGDQIRAMGDKATARKLAQEAGVPTVPGSKGTIQDLDEALAVAAEIGYPVIIKAT
                     AGGGGKGMRVAQDAEGFAQSFTLAQNEALSAFGNGSVYVEKYLARPRHVEIQVMGDTH
                     GRVVHLGERDCSVQRRHQKLIEEAPSPALTEALRERMGTAAVQLASRIGYVGAGTLEF
                     LLDTDGSFYFMEMNTRIQVEHPVTEMVTSFDLVKEQIRVAAGAPISFKGEGTRLRGHA
                     IECRINAEDPYRNFQPSPGAITAYHPPGGPGIRVDSHVYAGYHVPPYYDSLLAKVIAH
                     GNDRDEALARMRQALDSFVIEGVTTTIPFLARVIRHPDFAAGRIDTKFLEREPHLLRP
                     EA"
     gene            complement(1684..2880)
                     /locus_tag="VFU45_05915"
     CDS             complement(1684..2880)
                     /locus_tag="VFU45_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEU5358631.1"
                     /translation="MADAPAPKPAAAPGGAAAGPSFNFKGAITQMVQKNGSDLLLKVG
                     RPPTIRLNGELTPLEMPPLKPEDLKTLAEQIMTPRQVKEFAERKEADFAIGVPGVGRF
                     RTNVYQQRGTLAFAFRAIPYEVKTIKELLLPEVLDEIASKPRGMVLVTGVTGSGKSTA
                     LAAMINHINTTDRVNIITIEDPIEFLHRDQMANISQREVGTDTLSFQSALKSVLRQDP
                     DVILIGEIRDKETLDTALKAADTGHLVFSTLHTTDATQTINRIISFYPPHEHAEVRSL
                     LSTALAAVVCLRLVPKKDGSGRVPMCEVLINTAAVADNIRDLDKALNIPDLIAEGGVS
                     YGMQSFDQSLMKWYHNGDVSYESALFYASNPSEFALRASGIGSASDRTFADSGGKSGL
                     TQDLTP"
     gene            complement(2873..3355)
                     /gene="accB"
                     /locus_tag="VFU45_05920"
     CDS             complement(2873..3355)
                     /gene="accB"
                     /locus_tag="VFU45_05920"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU5358632.1"
                     /translation="MDISEIERLTRLLRESPEVGSIEVRGWFGRGVVITRYGSGGMMM
                     HAATPMAMAAPAGHAPGHSPHPPAPAAEKPAAPVANLKEIKSPMVGTFYKAPEPGAEA
                     YAKVGTRVSPGQTVCIIEAMKIMNEIEAEVGGVIKEVCVEDAQPVEFGQVLYRVDPNG
                     "
     gene            complement(3388..>4008)
                     /locus_tag="VFU45_05925"
     CDS             complement(3388..>4008)
                     /locus_tag="VFU45_05925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5358633.1"
                     /translation="AEVLPGVRVGESELAVAARLEAALRVRGSEWHPFPTIVASGPRA
                     ALPHARSTERTIAPGEWLLIDFGAQVDGYCSDLTRTVVVGKADTRQRAVYEVVEAAQR
                     RAREGVRAGMTGREADALARDLIAQRGYGEAFGHSLGHGLGLEVHEAPRLSPTAEAVL
                     PEGAVVTIEPGIYLPGWGGVRLEDDVLLAPEGPRCLSDGRTSLIEL"
BASE COUNT          552 a         1432 c         1405 g          619 t
ORIGIN      
        1 gaagagcgcc ggccccaggt cgagcagggt gccgatgaag agggggatga ggaccgcgag
       61 cccgcccacc aggagggcgg tgcgcttcat gtcgagccgc tcgaaccagc cgaacccggc
      121 cgccgcgacg ccgagcccca cgagcgggaa cccgatggcg ccggcgccca gcgccttcca
      181 caggatcgaa ccgacggtgg cggggagggt gccggcgccc gggatgggca ggagggcgat
      241 gccgagcgag aggcccagca ccagcgacgc gacggccccg atgcggcggc gggcgtcgtc
      301 actcatgcgg cgccccccgg gatcatcacg cctcggggcg caggaggtgc ggctcccgct
      361 cgaggaactt ggtgtcgatc cggccggcgg cgaagtcggg gtgccggatg acccgcgcca
      421 ggaacgggat ggtggtggtc acgccctcga tgacgaagct gtcgagcgcc tggcgcatcc
      481 gggccagcgc ctcgtcgcgg tcgttcccgt gggcgatgac cttggcgagg agcgagtcgt
      541 agtacggcgg cacgtgatac cccgcgtaca cgtggctgtc cacccggatc ccgggccccc
      601 ccggaggatg atacgccgtg atggcgccgg ggctcggctg gaagttccgg tacgggtcct
      661 cggcgttgat gcggcactcg atggcgtggc cgcggagccg ggtgccctcg cccttgaagc
      721 tgatcggcgc gccggccgcc acccggatct gctccttgac caggtcgaag ctcgtcacca
      781 tctcggtgac cgggtgctcc acctggatcc gggtgttcat ctccatgaag tagaacgagc
      841 cgtcggtgtc gagcaggaac tcgagggtgc cggcgccgac gtagccgatc cgggaggcca
      901 gctggaccgc cgccgtcccc atccgctccc gcagcgcctc ggtgagcgcc gggctcggcg
      961 cctcctcgat gagcttctgg tggcggcgct ggaccgagca gtcgcgctcg cccaggtgga
     1021 cgacgcggcc gtgcgtgtcg cccatgacct ggatctcgac gtggcgcggg cgcgcgaggt
     1081 atttctcgac gtacacgctg ccgttgccga aggcggagag cgcctcgttc tgcgccaggg
     1141 tgaacgactg cgcgaacccc tcggcgtcct gcgccacccg catccccttg ccgccgccgc
     1201 cggcggtcgc cttgatgatg accgggtagc cgatctccgc cgccaccgcg agcgcctcgt
     1261 ccaggtcctg gatggtgccc ttggagcccg gcaccgtcgg cacccccgcc tcctgcgcca
     1321 gcttccgcgc ggtggccttg tcgcccatgg cgcggatctg gtcgccggag gggccgatga
     1381 acgtgatgtt cgacgccttg caggtgtcgg agaactccgg gttctcggcc aggaagccgt
     1441 accccgggtg gatggcgtcg gcgccggtga tctcggcggc ggcgatcagg ctcgggatgc
     1501 ggaggtagga ctggcggctg ggcggcgggc cgatgcagac gtcgtcgtcg gcaaagcgca
     1561 cgtgcagcga ctcgcggtcg gcctcggaat agacggccac cgtctcgacg ccgagttccc
     1621 ggcaggcgcg gatgacgcgg agggcgatct ccccgcgatt ggcgatgagg actttcttga
     1681 acatcagggc gtgagatcct gggtcagtcc ggacttgccg cccgagtcgg caaaggtgcg
     1741 gtccgacgcc gagccgatcc ccgacgcccg gagcgcgaac tcgctcgggt tgctggcgta
     1801 gaagagcgcg ctctcgtagg acacgtcgcc gttgtggtac cacttcatca gcgactggtc
     1861 gaacgactgc atcccgtagg agacgccccc ctcggcgatg aggtcgggga tgttgagcgc
     1921 cttgtcgagg tcgcggatgt tgtcggccac ggcggcggtg ttgatgagca cctcgcacat
     1981 cggcacccgg ccgctgccgt ccttcttggg caccaggcgc aggcacacca ccgccgcgag
     2041 cgcggtggac aggaggctcc gcacctcggc gtgctcgtgc ggcgggtaga acgagatgat
     2101 ccggttgatc gtctgggtgg cgtcggtggt gtggagcgtc gagaacacga ggtgccccgt
     2161 gtcggccgcc ttgagcgcgg tgtcgagggt ctccttgtcc cggatctcgc cgatcaggat
     2221 gacatcgggg tcctgccgca gcaccgactt gagcgcgctc tggaaggaga gcgtgtcggt
     2281 ccccacctcg cgctgcgaga tgttggccat ctggtcgcgg tgcaggaact cgatcgggtc
     2341 ctcgatcgtg atgatgttga cccggtcggt ggtgttgatg tggttgatca tcgccgccag
     2401 cgccgtcgac ttgcccgaac cggtgacgcc ggtgacgagc accatcccgc gcggcttgct
     2461 cgcgatctcg tcaagcacct cggggagcag gagctccttg atcgtcttga cctcgtaggg
     2521 gatggcccgg aaggcgaatg cgagcgtgcc gcgctgctgg tacacgttgg tccggaaccg
     2581 ccccacgccg gggacgccga tggcgaagtc cgcctccttg cgctcggcga actccttgac
     2641 ctgccgcggc gtcatgatct gctcggccag ggtcttcaag tcctccggct tgagcggcgg
     2701 catctccagc ggggtgagct cgccgttcag ccggatggtc ggcgggcgcc cgaccttgag
     2761 gagcaggtcg gacccgttct tctgcaccat ctgggtgatg gcgcccttga agttgaacga
     2821 cggccccgcg gccgcgcctc cgggggccgc cgccggcttg ggcgcaggcg cgtcagccat
     2881 tcgggtccac ccgatagagg acctggccga actcgacggg ctgggcgtcc tccacgcaca
     2941 cttccttgat gacgcccccc acctcggcct cgatctcgtt catgatcttc atcgcctcga
     3001 tgatgcagac ggtctgcccc gggctcaccc gggtgccgac cttggcgtag gcctccgccc
     3061 cgggctcggg cgccttgtag aaggtgccga ccatcggcga cttgatctcc ttgaggttgg
     3121 ccaccggcgc cgccggcttc tccgccgcgg gcgcgggcgg atgcggcgag tgaccgggcg
     3181 cgtggcccgc cggggccgcc atggccatcg gggtcgccgc gtgcatcatc atcccgccgg
     3241 acccgtaccg ggtgatgacg accccgcggc cgaaccaccc gcgcacctcg atcgacccga
     3301 cctcgggcga ttcgcgcagc aggcgcgtca gccgctcgat ctcggaaatg tccaaagccc
     3361 ctccttgagg gcgtatcgtc gcgttcccta cagctcgatg agcgaagtcc ggccatccga
     3421 caggcaccgc ggaccctccg gcgccagcag cacgtcgtcc tccagtcgca caccacccca
     3481 cccgggaaga tagatgcccg gctcgatcgt gaccaccgcg ccctcgggga gcaccgcctc
     3541 cgcggtgggg ctcaaccgcg gcgcctcgtg cacttccaac cccagtccgt gccccagcga
     3601 gtgcccgaac gcctcgccgt agccgcgctg ggcgatcagg tcgcgcgcca gcgcatcggc
     3661 ctcccgcccg gtcatccctg cccgcacccc ctcccgcgcc cgccgctgcg ccgcttcgac
     3721 cacctcgtac accgcccgct gccgggtgtc ggccttcccc accaccacgg tccgcgtcag
     3781 gtcggagcag tagccgtcca cctgcgcccc gaagtcgatg aggagccact ccccgggtgc
     3841 gatggtccgc tcggtgctcc gcgcatgggg gagcgccgcg cgcggccccg acgcgacgat
     3901 cgtggggaac ggatgccact cgctcccccg cacccggagc gccgcctcga gccgcgccgc
     3961 cacggccagc tcgctctccc ccacccgcac gcccggcagc acctcggc
//
LOCUS       DASUAP010000230         5759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_565155, whole genome shotgun sequence.
ACCESSION   DASUAP010000230 DASUAP010000000
VERSION     DASUAP010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565155"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFU45_05930"
     CDS             complement(<1..727)
                     /locus_tag="VFU45_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5358634.1"
                     /translation="MTASPLTSWTTGVRREVLPNGLTILVQRDDSAPVAAVVTHVRAG
                     FFDEPDEWAGLSHVLEHMFFKGTPTRGVGAVARETKGLGGYLNAGTGYDRTSYYVVVP
                     ARNLGRAIDIQADALQRATLDPGELARELQVIIEEARRKRDTPSAVASETVHELLFDR
                     HRIRRWRIGHEEALAKFTATELRAYYATRYVPSRTIVSIVAAVPEDEMLAAARAAYGG
                     WPARPADVPDGPTEPPRTGVRVRT"
     gene            complement(798..1916)
                     /locus_tag="VFU45_05935"
     CDS             complement(798..1916)
                     /locus_tag="VFU45_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5358635.1"
                     /translation="MSDPFMSSDDYDEQAHQLYNEGRYDDALALLREGLALYPHAVEL
                     HVGVAYAHLAREEYAWARRSFETATMLDPDHEDALAGYGEVLLKLGAADLALTQFERI
                     LLLGFSDDHELMLQIGRALFREGLMQSAHRFFELAVGAQPDSPEAAACVGYAAHRLGL
                     ESQAIYWLRRALEADPDYSEARIYLANMLYDRGESEAALHQLDRTRPDDHYDELGLWR
                     YVELKKTIYRLPDDDPELAPWLARLGEVSGEPDGIDTLLAEIESQQPDGSVRDPHQLE
                     LFGTLLTELQEMQKRPGASEQHQVSILSGHNFRGTWEEILLQMKAADQEWAHASLADF
                     MGSIARRGATETGVVIPLTDAEAFIRGSAEAGVLRILH"
     gene            2421..3071
                     /locus_tag="VFU45_05940"
     CDS             2421..3071
                     /locus_tag="VFU45_05940"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5358636.1"
                     /translation="MTTETQTPRWHRRPDARPEEILDAAAKVFGENGFARARLEDVAR
                     EAGVSKGTVYLYFDGKETLFREMVRAKIVSAVATAEEFARAYTGSARELMVEMVRGYW
                     RLMREEEMVRISRLCVSELGCFPELAKFYYDEVILRARRLIETVMERGVRSGEFRPVA
                     NNFLPRGLPMLVIQAAQAQCFFTGLDADRLSDQQVFDGVVDLLLHGVLARPADPGR"
     gene            3079..4395
                     /locus_tag="VFU45_05945"
     CDS             3079..4395
                     /locus_tag="VFU45_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5358637.1"
                     /translation="MRMRLVLIAAFAAAPSMARAQGPAAAPATLTLAGAVRLGQERAI
                     GAALARLGLRSSELRASEQTGHLLPTLDANGTYSRQTNNLATFGLSIPGFPVVTPDFN
                     LYTLEARASQVLFDGGLFNRIAAAHSSAGAARYDARAAADQAGAIAGLAYLRVLSAQE
                     TVAAREADSAVAADLLHQAQQLRAAGVSAAIDVTRAEVNAAAVRTQLLLARTGLERAR
                     IDLAQALDLPTDMTLVLADSLGGGALTVPVTPDSAVAYALAHRPEAAAERERSRVAEL
                     SRRAVLWENLPTLGLFGSYNYAGTRTDQMNGTYQVGIRASVPIFDGLRRQRRYAESGD
                     QVDAQALREHALLQQVEVDARTAVLDLQSARGQVALAGERLDLAEQELAQSDERFKAG
                     VAGSVETSNSQAGLIQARDAYIQAKVNFATARLRTYRALGALDQMP"
     gene            4421..5542
                     /locus_tag="VFU45_05950"
     CDS             4421..5542
                     /locus_tag="VFU45_05950"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU5358638.1"
                     /translation="MTATATPVEQSMTTSTKPDATPPVPAATPTNRMRLYIIGVVALA
                     LLTWGAKHLIYARSHEGTDNAQVDGHITPIAPKVSAFVSDVRVEDNQVVKAGDTLVVL
                     DDRDLKVRLAQAEADLAAARAQAGSSSRAGQAQAQLEAARASAASAASAVTSAEANFR
                     KASQDLERTKGLAAQQIVSAQALDAAQATYDATKANLTTAQRQANAAESQVAAAAAAL
                     TSADARLAAAQAAVDNARLQLGYTHVTSPLDGVIAKRSVEPGELIQVGQQLMAVVPLH
                     DVWVTANLKETQLTGVVPGARAEFSVDAYPGRTYTGHVESVSPATGARFALIPPDNAT
                     GNFTKVVQRIPVRIAVDGGVDPAHPLRPGMSVEVEISTR"
     gene            5553..>5759
                     /locus_tag="VFU45_05955"
     CDS             5553..>5759
                     /locus_tag="VFU45_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358639.1"
                     /translation="MATMALALPQRRSLSRAQVYEARYLIAFAVTLASVLELVDTSIV
                     NVAIPHMMGNLGATLDEIAWVSTGY"
BASE COUNT          829 a         2096 c         2032 g          802 t
ORIGIN      
        1 gggtgcggac ccggaccccg gtccgcggcg gctcagtggg gccgtcgggc acgtcggcgg
       61 gccgggcggg ccagccgccg taggccgcgc gcgccgctgc gagcatctcg tcctccggca
      121 ccgcggccac gatgctcacg atcgtccgcg aggggacgta gcgagtggcg tagtaggccc
      181 ggagctcggt cgccgtgaac ttcgcgagcg cctcctcgtg gccgatgcgc caccgccgga
      241 tgcggtgccg atcgaagagg agctcgtgca ccgtctccga ggccaccgcc gagggcgtgt
      301 cgcgcttgcg ccgcgcctcc tcgatgatga cctggagctc ccgggcgagc tcgccgggat
      361 cgagcgtggc ccgctgcagc gcgtcggcct ggatgtcgat ggcgcgcccg aggttccggg
      421 ccggcaccac cacgtagtag ctggtccggt cgtagccggt gccggcgttg agatagccgc
      481 cgagcccctt ggtctcgcgg gccaccgcgc cgaccccgcg ggtgggcgtg cccttgaaga
      541 acatgtgctc gagcacgtgc gagaggccgg cccactcgtc gggctcgtcg aagaagccgg
      601 cgcgcacgtg ggtcaccacc gccgccaccg gcgcggagtc gtcgcgctgg accaggatcg
      661 tcaggccgtt ggggaggacc tcgcgccgga cgccggtggt ccaggaggtc aggggggacg
      721 cggtcatcag gggatactaa acgcagcggg gcgtcctgtc tcggacgccc cgcgcgcgac
      781 gacgcgcctg caggtgctca gtgcaggatg cggaggaccc cggcctcggc gctcccgcgg
      841 atgaacgcct cggcgtcggt gagggggatg acgaccccgg tctcggtggc gccccgacgg
      901 gcgatcgatc ccatgaagtc ggccagcgag gcgtgggccc actcctggtc ggccgccttc
      961 atctggagga ggatctcctc ccaggtgccg cggaagttgt gcccggagag gatcgacacc
     1021 tggtgctgct ccgaggcgcc ggggcgtttc tgcatctcct gcagctccgt gagcagggtg
     1081 ccgaagagct cgagctggtg cgggtcgcgc accgagccgt cgggttgctg cgactcgatc
     1141 tcggccagca gcgtgtcgat gccgtcgggc tcccccgaca cttcgcccag gcgcgccagc
     1201 cagggagcca gctcggggtc gtcgtcgggg aggcggtaga tcgtcttctt gagctcgacg
     1261 tagcgccaca ggccgagctc gtcgtagtgg tcgtcggggc gggtacggtc gagctggtgg
     1321 agcgccgcct cggattctcc ccggtcgtag agcatgttgg ccaggtagat ccgcgcctcg
     1381 gagtagtccg ggtcggcctc gagggcccgc cgcagccagt agatggcctg gctctcgagc
     1441 ccgagccggt gcgccgcgta gcccacgcag gccgccgcct cggggctgtc aggctgggca
     1501 cccaccgcca gctcgaagaa ccggtgcgcc gactgcatca gcccctcgcg gaagagcgcg
     1561 cggccgatct ggagcatcag ttcgtggtcg tcggagaagc cgagcaggag gatacgctcg
     1621 aactgggtca gcgcgaggtc ggcggcgccg agcttgagga gcacctcgcc gtagccggcg
     1681 agcgcgtcct cgtggtcggg gtcgagcatc gtcgccgtct cgaaggagcg ccgggcccac
     1741 gcatactctt cccgcgcgag gtgcgcatac gcgacgccga catggagctc gacggcgtgg
     1801 ggatagaggg cgagtccttc gcggagcaac gctagtgcgt catcgtacct gccttcgttg
     1861 tacagttggt gggcctgttc atcgtagtcg tcggaactca tgaacgggtc cgacatcggc
     1921 aggcgctcct ttgagagcgt gtgggtctaa gctaggaaag agcgccgggc ggggcaaggg
     1981 gcgggcggag cgcgtgagtc gggtgaactg tcgcgtggaa ggccgcgttc actgccgcat
     2041 caacggccgg gtggggtgtc cgacccatcg cggatggcgg cagatccctt cgcgccgcgc
     2101 acgtcgttcg attccacgcc gcagatctct cgcccatcga accggcagtt cgcgcggcgg
     2161 ttcccgcggt cggtcacacg ccaatcctcg cggcagttca cccggcactc cacgcggcag
     2221 ttcgcgcggc ggttcccgcg gtcggtcaca cgccaatcct cgcggcggtt caccctgcag
     2281 tccacgcggc agttcacgag gtgttgcacg cggccgttca cccgccactc cccctcccct
     2341 tgactaactg accagtcatt tagatgttac tggccgttcg gtaagtaact cgttgatgta
     2401 actcattaca ggataacgag atgacgaccg agactcagac tcccaggtgg catcgacgtc
     2461 ccgatgcacg tccggaagag atcctcgacg ccgcggcgaa ggtgttcggg gagaatggct
     2521 tcgcccgtgc ccgcctggag gacgtcgcgc gcgaggcggg cgtcagcaag ggcaccgtct
     2581 acctctattt cgacggcaag gaaacgctct tccgcgagat ggtccgcgcc aagatcgtct
     2641 cggcggtggc cacggcggag gagttcgccc gcgcctacac cggcagcgcc cgcgagctga
     2701 tggtcgagat ggtgcgcggc tactggcggc tgatgcgtga ggaagagatg gtgcggatct
     2761 cccggctctg cgtctccgag ctcggctgct tccccgagct cgccaagttc tactacgacg
     2821 aggtgatcct ccgcgcccgc cgtctcatcg agacggtgat ggagcgcggg gtccgctccg
     2881 gcgagttccg tccggtggcc aacaatttcc tcccgcgcgg cctgccgatg ctggtcatcc
     2941 aggccgcgca ggcgcagtgc ttcttcaccg gcctcgatgc cgaccgcctg tcggaccagc
     3001 aggtgttcga cggcgtcgtc gacctgctcc tccacggcgt cctcgcccgt cccgccgacc
     3061 ccggccgctg acactcccat gcgcatgcgc cttgtcctga tcgcggcatt cgccgccgcc
     3121 ccgtcgatgg cccgggccca gggccccgcc gccgctccgg ccaccctcac gctcgccggc
     3181 gccgtgcggc tcggccagga gcgcgccatc ggggccgccc tggcgcgcct cggcctccgg
     3241 tcctcggaac tgcgggcgtc ggaacagacc ggccacctgc tcccgaccct cgacgccaac
     3301 ggcacctatt cccgccagac caacaacctg gccacgttcg gcctgtcgat cccggggttc
     3361 ccggtggtca cgcccgactt caacctctac acgctcgagg cccgggcgtc gcaggtgctc
     3421 ttcgacggcg gactcttcaa ccggatcgcc gccgcgcaca gctccgccgg cgccgcccgg
     3481 tatgacgcgc gcgcggccgc cgaccaggcg ggtgcgattg ccgggctcgc gtacctccgg
     3541 gtgctgagcg cccaggagac cgtcgccgcg cgcgaggcgg acagcgccgt cgccgccgac
     3601 ctcctgcacc aggcccagca gctccgggcc gccggggtga gcgccgcaat cgacgtcacg
     3661 cgcgccgagg tgaacgccgc cgccgtccgc acccagctgc tgctggcgcg gaccggcctc
     3721 gagcgggcgc gcatcgacct ggcccaggcg ctcgacctgc cgaccgacat gacgctcgtc
     3781 ctggccgact cgcttggcgg cggcgcgctc accgtgccgg tgacgccgga ctccgccgtg
     3841 gcgtacgccc tggcccaccg ccccgaggcc gccgccgagc gggagcggag ccgggtggcc
     3901 gagctgagcc gccgcgcggt cctgtgggag aacctcccca ccctgggcct cttcggcagc
     3961 tacaactacg ccggcacccg gaccgaccag atgaacggca cgtaccaggt cggcatccgg
     4021 gcgtcggtcc cgatcttcga tggcctgcgg cgccagcgcc gctacgccga atcgggcgac
     4081 caggtcgacg cgcaggcgct ccgcgagcac gcgctgctcc agcaggtgga ggtggacgcg
     4141 cgcacggccg tcctcgacct gcagagcgcc cgtggccagg tggccctggc cggcgaacgc
     4201 ctcgacctgg ccgagcagga gctggcgcag tccgacgagc gcttcaaggc gggcgtcgcc
     4261 gggagcgtgg agaccagcaa ctcgcaggcg ggcctcatcc aggcccgcga cgcctacatc
     4321 caggcgaagg tcaacttcgc caccgcgcgc ctgcggacgt accgcgcgct cggcgccctc
     4381 gaccagatgc cctgaccggc acgcacggaa caccgatccc atgaccgcca ccgccactcc
     4441 cgtggagcag tcgatgacca cgagcaccaa gcccgacgcc acgccgccgg tacccgccgc
     4501 cacgcccacg aaccggatgc ggctctacat catcggggtc gtcgccctgg ccctcctcac
     4561 gtggggggcc aagcacctga tctacgcccg cagccacgag ggcaccgaca acgcccaggt
     4621 cgacgggcac atcacgccca tcgcgcccaa ggtgtcggcc ttcgtctccg acgtgcgggt
     4681 ggaggacaac caggtcgtga aggcgggcga caccctcgtg gtgctcgacg accgcgacct
     4741 caaggtccgg ctcgcccagg ccgaggccga ccttgcggcc gcgcgggcgc aggcggggag
     4801 cagcagccgc gccggccagg cgcaggccca gctcgaggcc gcgcgggcgt ccgcggcgag
     4861 cgccgcgagc gccgtgacat cggcggaggc gaacttccgc aaggcgtcgc aggacctcga
     4921 gcggaccaag ggcctggccg cacagcagat cgtgtccgcg caggcgctcg atgcggcgca
     4981 ggccacgtac gacgccacga aggccaacct caccaccgcc cagcggcagg ccaacgccgc
     5041 cgagagccag gtggccgcgg cggcggcggc gctcaccagc gccgatgcgc ggctcgccgc
     5101 cgcgcaggcg gcggtcgaca acgcccggct ccagctgggc tacacgcacg tgacctcgcc
     5161 gctcgacggc gtgatcgcca agcgctcggt cgagcccggc gaactgatcc aggtcgggca
     5221 gcagctgatg gcggtggtgc cgctccacga cgtgtgggtc acggccaacc tcaaggagac
     5281 ccagctcacg ggcgtggtgc ccggcgccag ggccgagttc tccgtcgacg cctacccggg
     5341 gcggacctac accgggcacg tcgagagcgt gagccctgcg acgggcgccc gcttcgcgct
     5401 catcccgccc gacaacgcca ccggcaactt caccaaggtg gtgcagcgga tcccggtgcg
     5461 catcgcggtg gacggcggcg tcgacccggc gcacccgctg cgcccgggga tgtcggtcga
     5521 ggtcgagatc tccacccgct gaggccgcgc tgatggcaac gatggccctg gccctgcccc
     5581 agcggcggag cctgtcccgg gcgcaggtct acgaggcccg gtacctcatc gcctttgcgg
     5641 tgacgctcgc gagcgtgctc gagctggtgg acacgagcat cgtgaacgtg gcgatcccgc
     5701 acatgatggg gaacctcggc gccaccctcg acgagatcgc gtgggtgagc accgggtac
//
LOCUS       DASUAP010000231        13796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_74780, whole genome shotgun sequence.
ACCESSION   DASUAP010000231 DASUAP010000000
VERSION     DASUAP010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 13796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13796
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74780"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFU45_05960"
     CDS             complement(<1..180)
                     /locus_tag="VFU45_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5358640.1"
                     /translation="MLSRPDALALMHEYTANEALRRHMYAVELAMRAMAQRAGEDPEA
                     WGTVGLLHDFDYERFP"
     gene            complement(362..1612)
                     /locus_tag="VFU45_05965"
     CDS             complement(362..1612)
                     /locus_tag="VFU45_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU5358641.1"
                     /translation="MHRHRGIILALGLFGLTAGCFSNEKLNSPSAPTGGTLFSRMVFM
                     GNSITAGFQSAGINDSTQRQSYAFLVAQAAGAPYYYASLQGRGCPAPFDDNSTQHRVG
                     GGGPTDCDLRAPTPLPWLSNVAVPGARTVETFDNFATPVSASNALTTFILGGRTQVQA
                     MRAANPTLVTVWIGANDVLASLTNSGNPGTPLAVTPSDSFNAEYDILAAQLDSSGAKV
                     VLIGVPNVTDIPYTSPGAIYWCLKNAPACGPAFGASSPFPPTFAVTLSCAPGAAVPGE
                     VGDSILVPWTVGVPLIGAAALGQSDTLDCANDLQVVTAAEHDGLNAAVDADNAKISAV
                     AAAHHWAYFDPNPTLLALKADPTKVAPFPSLPGTTGNPGPNVLFGSMFTLDGVHPSAA
                     AHKIIADSLVATINTAYGTSVPLP"
     gene            complement(1631..2995)
                     /locus_tag="VFU45_05970"
     CDS             complement(1631..2995)
                     /locus_tag="VFU45_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEU5358642.1"
                     /translation="MRRFQVWLAAGILAGAALATAPSSAEAQGYGLYEHNACAMARGG
                     TGVASPCADGSAMFFNPAGLANVSGKVGSLGGVLINTSGKFTDDITGRSSDLKNGHYP
                     APNVYLAMPLGTKFAVGLGLFAPYGLTTEWPTTSEGRFLGVKSKIASIYLQPTLAMKL
                     NDRWNVGAGFDLNFMSVELDQRLDLSSQLAAPSVTFASLGIPAGTDFAAAALSGNATG
                     VGYHVGLQFKASDRVSFGLRYMSRQLVKFNSATADFTQVSTGIILPAGNPFGVPAGTP
                     LDSVVAPQFRGTGPLQNQGGKTWLRVPEQLVLGTAYKITPKLTGLFDLQWTNWDVFDA
                     LTLEFTKLPTVVLPEGYKGVVDWRLGAEYQLKPSTVLRAGIIAHKGAAPDQNVTPNLP
                     EGSRTELSLGAGTELTPQMGLDLAFQLIDQADRRGRTTATPGTNGLYAFNAYLIGASL
                     VYRF"
     gene            complement(3116..3535)
                     /locus_tag="VFU45_05975"
     CDS             complement(3116..3535)
                     /locus_tag="VFU45_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358643.1"
                     /translation="MQIVLAALADYALVDQAGRLSVVGIFSQVWVSQFPAEHRRTHLV
                     IRVIGRRTEIGEHKIRIRFLDPDGRELFGGEGTFRFGEPPAGVTDVEAAVILAYDLPL
                     PRAGRYDVELTLDAETSTHLPLAAALVPAPPAPLPNG"
     gene            complement(3535..3981)
                     /locus_tag="VFU45_05980"
     CDS             complement(3535..3981)
                     /locus_tag="VFU45_05980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5358644.1"
                     /translation="MKTATIDTARGVITAELFDDEAPKTVANFEKLANDGFYDGTRFH
                     RVIPNVVIQGGDPLSKDPNNPRVGTGGPGYTIKCETDRNTHKHVAGTLSMAHAGRDTG
                     GSQFFIAHSPQPHLDKVHTVFGQVTKGMDVVNAIKANDVVNSIRVA"
     gene            complement(4130..6115)
                     /locus_tag="VFU45_05985"
     CDS             complement(4130..6115)
                     /locus_tag="VFU45_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5358645.1"
                     /translation="MTRRIAAIAVLCLAASRAGRAQAPVDSGVFVLHKMKQAIGRESY
                     RATRSDSAVVIVDSFAFTDRGQRVPLATTLALGPDLTPRHLETSGKVSRLSRIDDAVE
                     RIGDSIRVRVDSISQVVVAPATWFAVTGYAPVIGQQEMLRYWRAHGRPDSLRAVPAGW
                     IRITDRGVDTVTGPAGPRALTRYDIDGLIWGRETAWTDADGLIIGVVTVDAEFDHFEA
                     LRAGWEDALGFFVHRAAQDAAAALVTATKDAVAGTGGFAIVGATLIDGTGRPAVPNAT
                     VVVRGGRIAAAGARVRVPKGMPELDGRGLSVLPGLWDMHAHFEQVEWGPIYLASGVTT
                     VRDVGNEFDFITAVRDAIAAGRGVGPRILAAGVVDGDGPFALGVERVTDSASAAAEVR
                     KYHDAGFQQMKLYSSLTPASVAAVTRDAHRLAMTVTGHIPEGMTAYDGVRGGMDQINH
                     VQYIYPLMFDSTPAGTPRPPLDTASAAAHRIEAFLVRHHTVLDPTLSIFEWALHPANV
                     PFEHYEPGVLKVAPQLAALLEHAGTPPADSARYDRVFREMVTTVAALHRAGVPIVAGT
                     DQTIPGYSLHRELELYVQAGFTPMEAIQAATIVPARVMGVAKDVGTVTVGKRADLLLV
                     AGDPLQDFSALRRVRLVVQGGRRYEPAPLWRSVDFTP"
     gene            complement(6132..6512)
                     /locus_tag="VFU45_05990"
     CDS             complement(6132..6512)
                     /locus_tag="VFU45_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEU5358646.1"
                     /translation="MHGFKGERTLMRIHLGERDKWKGKPLHEAIVELLRARGYAGATV
                     FRAVMGFGASSHVRSDKIELLSLDLPMVVECVDTEEKIQAILPELDGMIGGGLITLER
                     ARVIMYRADVPPERRTDSWPIDRT"
     gene            complement(6563..8623)
                     /locus_tag="VFU45_05995"
     CDS             complement(6563..8623)
                     /locus_tag="VFU45_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5358647.1"
                     /translation="MNHLASSRSPYLSSAAHQPVHWYPWGPEAFAAARTSGRPILLDI
                     GAVWCHWCHVMDGESYEDPSLAELLNAEFVCVKVDRDERPDVDARYQRAVQALTGQGG
                     WPLTAFLSAAGEVFYGGTYFPPEGHHGRPGFRTVLTEVARIHREEKDRVAAQALAIRR
                     TLAHPQDGPEPVDPTVALLEQAEAELARHYDPRHGGFGRSPKFPHPAAVCFLIDRWCD
                     SGQAAPREMAAGTLRGMARGGFHDQLGGGFHRYSVDAEWIVPHFEKMAYDNAELLKAY
                     LDGHSAFGDPYFAEVALDTLRWIREVLADPDGGFGASQDADVGLEDDGNYFTWTADEA
                     RAVLSVEEFEVAAAHYDIGTAGEMHHDPARNVLWVAEEVGAIAHRTGRSAAETANLLA
                     LAQEKLKAARRLRPAPFVDRTRYTGWNAMLAGALLRAGAHLNDRWATRHALATLDRIR
                     RECARDAAVLHVAGGIGGLLDDQVQVAAAALDAFEAIGDPAWLTWAERLMEQAWTDHR
                     DDHAGGLFDVARTRGGEGLLPERTKPVQDSPTPSPNGVAALNCARLGALLDSGTWRER
                     HLELVQVFGGQAAELGLFGATFLRALEWAVLPVTHLVVVGAVGDAEADRMHRTALTTC
                     IPRKVVHRLTGADAGRPLPAALRAMVDGQAPRAYACRGETCDAPAAEDAAWTATLARL
                     AKPR"
     gene            complement(8745..9527)
                     /locus_tag="VFU45_06000"
     CDS             complement(8745..9527)
                     /locus_tag="VFU45_06000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5358648.1"
                     /translation="MTSPALAWSQVARAYQRNILPGFTPAAAALCTALRVGSGDRVLD
                     IACGPGTLSLAALRQGASQVTGVDFAAEMVSLAQAATIGRSAEFFLGDATALPVPDEA
                     FDVGASSFGVIFAPDPRRAMSELHRALAPGGRAGLLAWLRGDTTNAYYDRVYRHIPRP
                     QTAHDPYAWGDRAIARKWFGEWFGYLELSTITVPYEGPTPAAVWEVLRSSTGRVAVSY
                     AALDRSAQERMDREMIDFFERFRRPDGGVYWPREALMITGVK"
     gene            complement(9673..10284)
                     /gene="nth"
                     /locus_tag="VFU45_06005"
     CDS             complement(9673..10284)
                     /gene="nth"
                     /locus_tag="VFU45_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU5358649.1"
                     /translation="MFSLLHRAYPDAHCALDHRNAYQLLAATILSAQCTDVRVNLVTP
                     ELFRAYPTPSALAGARQDDVERLIRSTGFFRNKAKNLIAMAQALVAEHGGRVPRTMAE
                     LHALPGVGRKTANVVLGNAFAINDGVVVDTHVGRLARLLGLTRHADPVKVERDLMDHF
                     PRAQWTLLSHLLISHGRAVCIARRPRCGECVLAAICPSAQVPR"
     gene            complement(10320..12053)
                     /gene="polX"
                     /locus_tag="VFU45_06010"
     CDS             complement(10320..12053)
                     /gene="polX"
                     /locus_tag="VFU45_06010"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEU5358650.1"
                     /translation="MSAIDKDGVVETLEQVAALLELKGENAFRVRAFRGAARAVTALP
                     GTVAEAIADGSLGSAKGIGPATRQLIEELVATGRSALLEELRGQVAPGLLEMLQLPGL
                     GVAKVRQIHETLRIETIAELEAAAQDGSLAALPRFGAKTAANILASIEFLRRSSAFRL
                     CHHAAAEAASLAATLAALPGVERVVTAGDVRRRMEVVNGLSFVVIGDTPPAEFFRRLA
                     AIPGIVEFSGHDERRVTLRHGGGSVAQVIVTTLKNAGAVLVRATGSDAHVAALEAHAR
                     ATGHTLDGAALWHGSTFVPTPDEATFYRALGLEETPPELREGTDEIAAARDGVLPTLV
                     TRADLRGFLHCHSNWSDGTTPIEDLARACQAAGYAYVGLTDHSQAAAYAGGLKPDALR
                     RQADEIDAVNARLTGVRVLKGIEADILADGALDYPAAVLDRLDFVIGSVHSRFQMGRD
                     EMTARMLRALDDPHLTIVGHPTGRLLLAREAYAVDLDALIEKAAAVGAAIEINADPHR
                     LDLDWRVLRSARARGLTISIGADAHDVGGIGNMEWGVGIARKGWLTAADILNTRSVEE
                     FLAFARKRRGA"
     gene            complement(12050..12697)
                     /locus_tag="VFU45_06015"
     CDS             complement(12050..12697)
                     /locus_tag="VFU45_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358651.1"
                     /translation="MAERPDPFTLVFGQMADDRFPALAASLLAAGRDEADRDAFLLDR
                     AVVELLQDLVPEEHDVDQIEEFVAVLHHAYLFWRNGRPVVAVDRSRATTLLSPAWPDT
                     ALTVRPSAYHQFPERLVWAQLSPDAPHEPLDGFFVRPAPAAAIEVLGVFGVHPGRPGF
                     GVAEVAGPRPLSLRRADGSPPFSPALPGGDAAGLHSLVGADELLELAFRAGGEAP"
     gene            complement(12690..13550)
                     /locus_tag="VFU45_06020"
     CDS             complement(12690..13550)
                     /locus_tag="VFU45_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5358652.1"
                     /translation="MTPITSPVETFTRHVNGIHIFERRSGSGPAVVALHGGPGASHDY
                     LLPGFDALAEGRTLVLYDQRGGGRSPLPREIKAGWEEHVADLHALKELWGDQPHDLIG
                     YSWGGLLAMLYAIRHPCSVARLALVSPAPSYREARLEYERRYARITNGEWAQAERRRL
                     RESGLQSSDLAAYQRRLFELAVIAYFKDPSRVAELSGFRLVERVQKEVWASLGEYDLR
                     PALRKLEIPTVVLHGADDVIPWESARETADCLGAEFHLIPDCGHVPYIEGFETFRRVL
                     DAFLPRTGRG"
BASE COUNT         2000 a         4508 c         5277 g         2011 t
ORIGIN      
        1 ggggaagcgc tcgtagtcga agtcgtggag caggcccacc gtgccccacg cctccgggtc
       61 ctcgccggcg cgctgcgcca tggcgcgcat ggccagctcg acggcgtaca tgtgccggcg
      121 gagcgcctcg ttggcggtgt actcgtgcat cagggcgagg gcgtcagggc gggagagcat
      181 ctcggtccgg gctccacggt tcgggcgtca agcactattg cctgcgagca tcgcttcagt
      241 acattcggcc acttctcact tctcacttct cacttctcac ttctcacttc ctccattcta
      301 acctgaacga acgccccgcg aacctctcgg tccgcggggc gttgtttgtc cgatcacggc
      361 gtcagggtag cggaacggag gtgccgtagg cggtgttgat cgtcgccacc agtgagtcgg
      421 cgatgatctt gtgcgccgcg gcgctcggat gcacgccgtc gagggtgaac atcgagccga
      481 agagcacatt cggcccgggg ttccccgtgg tcccgggaag gctcgggaac ggcgcgacct
      541 tggtcgggtc ggccttgagg gccagcagcg tggggttcgg gtcgaagtag gcccagtgat
      601 gcgctgccgc caccgccgag atcttggcat tgtcggcgtc caccgccgca ttcagcccat
      661 cgtgttcggc tgccgtgacc acctgcaggt cgttggcgca gtcgagcgtg tcgctctggc
      721 cgagcgcggc cgcgccgatg aggggtacgc cgaccgtcca cggtacgagg atcgagtcgc
      781 ccacttcgcc cgggaccgcg gcgcccggcg cgcagctgag ggtcacggcg aacgtcggcg
      841 ggaagggtga cgacgcgccg aaggccggtc cgcaggccgg cgcattcttg aggcaccagt
      901 agatcgcgcc gggcgacgtg tacgggatgt cggtcacgtt cggcacgccg atcaggacca
      961 ccttggcgcc ggacgagtcg agctgggcgg ccaagatgtc atattcggcg ttgaacgagt
     1021 cgctcggcgt cacagccagc ggcgtgcccg gattcccgct gttggtgagt gaggccagca
     1081 cgtcattggc tccgatccac accgttacca gggtcgggtt cgccgcgcgc atcgcctgga
     1141 cctgggtgcg tccgccgagg atgaacgtgg tgagcgcgtt cgaggcggag accggggtgg
     1201 cgaagttgtc gaacgtctcg accgtccgcg cccccgggac ggcgacgttg ctgagccagg
     1261 gcagcggtgt gggcgcgcgc aggtcacagt cggtgggacc gccgccgcct acccggtgtt
     1321 gggtggagtt gtcgtcgaac ggcgcgggac agccgcgccc ctggagcgag gcgtagtagt
     1381 acggtgcgcc ggcggcctgc gcgaccagga aggcgtacga ctgccgctgg gtgctgtcat
     1441 tgatcccggc cgactggaac ccggcggtga tgctgttgcc catgaacacc atccggctga
     1501 acagggtgcc gccggtgggt gcggacgggg agttgagctt ctcgttgctg aaacagccgg
     1561 ccgtgagccc gaagaggccc agggccagga tgataccgcg atgacgatgc atcatggctg
     1621 ggtctccgga tcagaagcgg tagacgaggg acgcgccgat gaggtacgcg ttgaaggcgt
     1681 acagcccgtt ggtgccgggc gtggcggtgg tccgcccgcg ccggtcggcc tggtcgatga
     1741 gctggaacgc gaggtcgagc cccatctgcg gggtgagctc ggtcccggcg ccgaggctca
     1801 actcggtccg gctcccctcg gggaggttgg gggtcacgtt ctggtcaggc gcggcgccct
     1861 tgtgggcgat gatgccggcg cggagcaccg tggagggctt gagctggtac tcggcgccca
     1921 gccgccagtc gacgaccccc ttgtagccct cgggcagcac cacggtcgga agcttcgtga
     1981 actcgagggt cagcgcgtcg aagacgtccc agttggtcca ctgcaggtcg aagaggccgg
     2041 tgagcttcgg cgtgatcttg tacgccgtgc cgagcaccag ctgttcgggt acgcggagcc
     2101 aggtcttgcc gccctggttc tggagcgggc cggtgccgcg gaactggggc gccaccaccg
     2161 agtcgagcgg ggtgccggcc gggacgccga acgggttccc tgccgggagg atgatcccgg
     2221 tggacacctg ggtgaagtcg gccgtggcgc tgttgaactt gaccagctgg cgtgacatgt
     2281 accggaggcc gaatgagacc cggtcagagg ccttgaactg gaggccgacg tggtagccga
     2341 cgccagtcgc gttgccggag agtgccgcgg cggcgaagtc ggtccccgcg gggatcccga
     2401 ggctggcgaa ggtgacgctg ggcgccgcga gctggctcga gaggtcgagc cgctggtcga
     2461 gttcgacgct catgaagttg aggtcgaagc cggccccgac gttccaccgg tcattgagct
     2521 tcatcgcgag cgtgggttgc aggtagatgc tggcgatctt gctcttgacg ccgaggaagc
     2581 gcccctcgga cgtggtgggc cactcggtgg tgaggccgta tggcgcgaag aggccgaggc
     2641 ccacggcgaa cttggtcccc agcggcatgg cgaggtagac gttcggcgcc gggtagtggc
     2701 cgttcttcag gtcgctcgac cggccggtga tgtcgtcggt gaacttgccg gaggtgttga
     2761 tgagcacccc gcccaggctc ccgacctttc cggagacgtt ggcgaggccg gccggattga
     2821 agaacatggc ggagccgtcg gcgcaggggg atgccacccc ggtgccgcct cgggccatgg
     2881 cgcatgcgtt gtgttcataa agcccgtagc cctgggcctc cgcgctcgaa ggtgccgtgg
     2941 cgagcgcggc gcccgcgagg atcccggcgg ccaaccagac ctggaaccgt cgcatagaga
     3001 tgctccgaat gagggtatgg caggtgctgt aactgatggt acgactgggg ctttctagtc
     3061 tacgggcccc cgaggggttt gtcaaaagcc tgtcgtgaca ggtttcagga tgtcttcagc
     3121 cgttgggaag tggagcgggc ggcgcgggca cgagggcggc ggccaggggg agatgggtcg
     3181 aggtctccgc atccagcgtg agctccacgt cgtatcgacc ggcccggggg aggggcaggt
     3241 cgtaggcgag gatcacggcg gcctcgacgt ccgttacgcc cgccggcggc tcgccgaagc
     3301 ggaaggtccc ttcgcccccg aagagctcgc ggccgtcggg gtcgagaaag cggatccgga
     3361 tcttgtgctc gccgatctcg gtgcggcgcc cgatgacccg gatgacgagg tgggtgcgcc
     3421 ggtgctcggc ggggaactgg gacacccaca cctggctgaa gatccccacg acggagagcc
     3481 gccccgcctg gtcgaccagc gcgtagtcgg ccagggcggc cagaacgatc tgcatcaggc
     3541 gacgcggatg gagttcacca cgtcgttggc cttgatggcg ttcaccacgt ccatcccctt
     3601 ggtcacctgc ccgaacaccg tgtgcacctt gtcgagatgg ggctgggggg aatgcgcgat
     3661 gaagaactgg ctgccgccgg tgtcgcggcc ggcgtgggcc atcgagaggg tgccggcgac
     3721 gtgcttgtgg gtgttccggt cggtctcgca cttgatggtg tagccggggc cgccggtgcc
     3781 gacgcgggga ttgttgggat ccttggagag gggatcgccc ccctggatga cgacattcgg
     3841 gatgacgcga tggaagcggg tgccatcgta gaagccgtcg ttggcgagct tctcgaaatt
     3901 ggcgacggtc ttgggcgctt cgtcgtcgaa gagctcggcg gtgatgacgc cgcgcgcggt
     3961 gtcgatggtg gcagtcttca tgggagtcgg ggaagagaga agtgaagagt gaaaagggaa
     4021 aagggtagaa gggtcgtgag ttgcgagacg taagacgggc gacctgcgac taacgactaa
     4081 cgacccacgc cccgctcgaa tctagccggg aggcgctgag ggcgcccggt cagggcgtga
     4141 agtcgacgct ccgccagagc ggggcgggct cgtagcgccg gccgccctgg accacgagcc
     4201 gcacgcggcg gagggcgctg aaatcctgca gcgggtcgcc ggccacgagc aggaggtcgg
     4261 cacgcttgcc gaccgtgacc gtcccgacgt ccttcgcaac gcccatcacg cgtgcgggga
     4321 cgatcgtggc cgcctggatg gcctccatcg gcgtgaaccc ggcctggaca tagagctcga
     4381 gttcccggtg caggctgtag ccggggatgg tctggtcggt gccggccacg atcgggacgc
     4441 cggcccggtg cagcgcggcc accgtggtca ccatttcccg gaagacccgg tcgtaccgtg
     4501 ccgaatcagc gggcggggtg ccggcgtgct cgagcagggc ggcgagttgg ggcgccacct
     4561 tgaggacccc gggttcgtag tgctcgaagg gcacgttcgc ggggtgcagg gcccattcga
     4621 agatgctgag cgtcgggtcg agcacggtgt ggtgcctgac caggaaggcc tcgatccggt
     4681 gggccgccgc cgaggcggta tcgaggggcg ggcgcggcgt gccggccggc gtggagtcga
     4741 acatcaaggg gtagatgtac tggacgtgat tgatctggtc catcccgccg cgcacgccgt
     4801 cgtatgcggt catcccctcg gggatatgcc cggtcacggt catcgcgagc cggtgcgcat
     4861 cgcgggtcac cgcggcgacg gacgccggcg tgaggctgct gtagagcttc atctgctgga
     4921 agccggcgtc gtggtacttc ctgacctcgg cagccgcgct ggcggagtcg gtgacccgct
     4981 cgacgccgag cgcgaacggg ccatcgccgt caacgacccc cgccgcgagg atccggggac
     5041 cgacgccccg gccggcggcg atggcgtcgc ggacggcggt gatgaagtcg aactcgttgc
     5101 cgacgtcgcg cacggtggtg acgcccgacg cgaggtagat cggaccccac tccacctgct
     5161 cgaagtgtgc gtgcatgtcc cagagccccg gcagcacgct cagcccgcgc ccgtcgagct
     5221 ccggcatccc cttcggtacg cggacgcggg cgcccgcagc cgcgatccgg ccgccgcgca
     5281 ctacgacggt ggcattcggg acggcgggcc ggccggtgcc gtcgatcagc gtggcaccca
     5341 cgatggcgaa gccgccggtg ccggcgacgg cgtccttggt ggcggtgacc agcgcggcgg
     5401 cagcgtcctg ggcggcgcgg tgcacgaaga agccgagcgc gtcctcccag cctgcacgca
     5461 gcgcctcgaa gtggtcgaac tcggcgtcga cggtcacgac cccgatgatc aggccgtcgg
     5521 cgtccgtcca cgccgtctcc cgcccccaga tgagcccatc gatgtcgtac cgggtgagcg
     5581 cgcgcggacc agcgggcccg gtgaccgtgt cgacaccgcg gtcggtgatc cgaatccagc
     5641 cggcggggac ggcgcggagc gaatcgggcc gaccgtgcgc gcgccagtag cggagcattt
     5701 cctgctggcc gatgaccggg gcgtagccgg tcacggcgaa ccaggtggcc ggggcgacga
     5761 cgacctggct gatgctgtcc acccggaccc ggatcgagtc gccgatccgc tccacggcat
     5821 cgtcgatccg cgagagccgc gagaccttgc cgctggtctc gaggtggcgc ggcgtgaggt
     5881 cggggccgag ggccagcgtc gtcgccagcg ggacccgctg gccgcggtcg gtgaaggcga
     5941 atgaatccac gatgacgacg gcggagtccg accgggtcgc gcggtaggat tcgcgcccga
     6001 tggcctgctt catcttgtgc agcacgaaca cgccgctgtc gaccggcgcc tgggcgcggc
     6061 cggccctcga ggcggcgagg cacagcacgg cgatggcagc gatgcggcgg gtcatctggg
     6121 ctctcgcgag gtcaggtccg gtcgatgggc cagctgtcgg tgcggcgttc cggggggacg
     6181 tccgcacggt acatgatgac acgggcgcgc tccagggtga tgagcccgcc gccgatcatc
     6241 ccgtccagct caggcaggat ggcctggatc ttctcctcgg tgtccacgca ttcgaccacc
     6301 atcgggaggt cgagcgaaag gagctcgatc ttgtcgctgc gcacgtgcga gctggcgccg
     6361 aatcccatca cggcacggaa caccgtggcg ccggcgtacc cacgcgcgcg gagcagctcc
     6421 acgatcgcct cgtggagggg cttgcctttc cacttgtccc gctcgccgag gtggatccgc
     6481 atcagcgtgc gctcaccctt gaacccgtgc atcgatccac ctcgctttcg gggcgtccgc
     6541 cgccgcccgt acgaccctaa ccctaccgcg gcttcgcgag ccgcgcgagg gtcgcggtcc
     6601 aggcggcgtc ctcggctgcc ggggcgtcgc aggtctcgcc gcggcaggca taggcgcggg
     6661 gcgcctggcc gtcgaccatc gcgcggaggg ccgccggcag cggccgtccg gcgtcggccc
     6721 ccgtcaggcg gtgcaccacc ttccggggga tgcaggtggt gagcgcggtg cggtgcatcc
     6781 ggtcggcctc ggcgtcgccc acggcgccga cgacgacaag atgcgtcacc ggcagcaccg
     6841 cccactcgag cgcgcgcagg aaggtggcgc cgaagaggcc gagctccgcg gcctggccgc
     6901 cgaacacctg gaccagctcc aggtgccgct cccgccacgt ccccgagtcg agcagggcgc
     6961 cgagccgagc gcagttgagc gccgcgacac cgttgggaga cggtgtgggc gaatcctgca
     7021 ccggcttggt gcgttcgggg agcagcccct cgccgccgcg ggtgcgcgct acgtcgaaga
     7081 gaccgccggc gtggtcgtcc cggtggtcgg tccaggcctg ctccatcagg cgctccgccc
     7141 aggtgagcca ggccgggtcg ccgatcgcct cgaaggcgtc gagcgccgcc gcggcgacct
     7201 gcacctggtc gtcgagcagc ccgccgatgc cgccggcgac gtggaggacg gcggcgtccc
     7261 gcgcacactc ccgccggatc cggtcgaggg tcgcgagggc gtggcgggtg gcccagcggt
     7321 cgttgagatg cgctccggca cggagcagcg cccccgcgag catcgcgttc cagccggtgt
     7381 agcgggtgcg gtcgacgaac ggcgcggggc ggagccggcg cgcggccttg agcttctcct
     7441 gcgccagcgc cagcaggttc gcggtctccg ccgccgaccg gccggtccgg tgcgcgatgg
     7501 cgccgacctc ctccgcgacc cacaggacat tccgcgcagg gtcgtggtgc atctccccgg
     7561 cggtcccgat gtcgtagtgc gccgcggcga cctcgaactc ctccacactc agaaccgcgc
     7621 gcgcctcgtc cgcggtccag gtgaagtagt tcccgtcgtc ttcgagtccc acgtccgcgt
     7681 cctggctggc cccgaagccg ccgtcggggt cggcgagcac ttccctgatc catcgcagcg
     7741 tgtcgagcgc cacctcggcg aagtacggat cgccgaaggc cgagtgcccg tcgagatagg
     7801 ccttgagaag ctcggcattg tcgtacgcca tcttctcgaa atgggggacg atccactccg
     7861 cgtcgacgct gtagcggtgg aagccgccgc ccagctggtc gtggaatccg ccgcgcgcca
     7921 tcccgcggag cgtcccggca gccatctccc gcggcgccgc ttggccggaa tcgcaccagc
     7981 ggtcgatgag gaagcacacg gcggcggggt gcgggaactt gggactgcgg ccgaacccgc
     8041 cgtggcgggg gtcgtagtgg cgggcgagct cggcttcggc ctgttcgagg agcgccaccg
     8101 tcgggtcgac cggctccggg ccgtcctgcg ggtgggcgag ggtccggcgg atggcgagcg
     8161 cctgcgcggc gacgcggtcc ttctcttccc ggtggatccg cgccacctcg gtgagtaccg
     8221 tccggaaccc gggccggcca tggtgcccct ccggcgggaa gtaggtgccg ccgtagaaga
     8281 cctcgccggc ggcgctcagg aacgcggtga gcggccatcc cccctggccg gtgagtgcct
     8341 ggacggcgcg ctggtaccgc gcatcgacgt cagggcgttc gtcgcgatcg accttgacgc
     8401 agacgaactc ggcgttgagg agctctgcga ggctcggatc ctcgtagctc tcgccgtcca
     8461 tcacgtggca ccagtggcac cagaccgcgc cgatgtcgag caggatcggg cgaccgctcg
     8521 tgcgcgcggc ggcgaacgcc tcaggccccc acgggtacca gtgcaccggc tggtgcgcgg
     8581 cggacgagag gtagggggag cgggaggaag cgaggtggtt cattctggat gcgggagacc
     8641 agagacggat cgcgggtggg acgcgtgtgg cgggtggcgg gcggcaccga cccgatcccc
     8701 gtcactcgat ccccactggt ctcccgcctc ccgccgtacg tttctcactt gacccccgtg
     8761 atcatcagcg cctcccgcgg ccagtacacc ccgccgtcgg ggcggcggaa gcgctcgaag
     8821 aaatcgatca tctcccggtc cattcgttcc tgggcgctgc ggtcgagcgc ggcgtaggac
     8881 accgccaccc gtccggtgga gctgcggagc acctcccaga ctgcggcggg tgtggggcct
     8941 tcgtacggca ccgtgatggt cgaaagctcg aggtagccga accactcgcc gaaccacttc
     9001 cgggcgatgg cgcgatcgcc ccaggcgtag gggtcgtgcg ccgtctgggg ccgggggatg
     9061 tggcgataca cccggtcgta gtaggcgttg gtcgtatcgc cgcggagcca ggccaggagt
     9121 cccgcgcgcc cgccgggcgc cagggcgcga tggagctcgc tcatcgcgcg gcgggggtcg
     9181 ggggcgaaga tgacgccgaa gctcgacgcg ccgacgtcga acgcctcgtc cggcacgggg
     9241 agcgcggtcg cgtcgcccag gaagaactcg gcgctccggc cgatcgtggc ggcctgcgcg
     9301 agggacacca tctcggcggc gaagtccacg ccggtgacct gcgacgcccc ctggcggagc
     9361 gccgcgagcg agagtgtccc cggcccgcag gcgatgtcga gcacccggtc gccggatccg
     9421 acgcggagcg cggtgcagag ggccgccgcc gcgggggtga acccggggag gatattgcgc
     9481 tggtacgcgc gggcgacctg agaccaggcg agagcggggg aggtcaaggg agaagtgaga
     9541 agtaagaagt aagaagtaag aagtgaaaag ggaaaaggga aaagggaaaa gggagaaggg
     9601 agcaggagac aggggacggg ctggccaacg gtcgaacgtg aatgggaagt tagggcctcg
     9661 cgggggtcgg cgtcagcggg ggacctgggc cgaggggcag atcgcggcga ggacgcattc
     9721 gccgcagcgg ggtcgtcggg cgatgcagac ggcgcgcccg tgggagatga gcaggtgcga
     9781 gagcagggtc cactgcgcgc gggggaagtg gtccatcaga tcgcgctcga ccttgacggg
     9841 gtcggcgtgg cgcgtgaggc cgaggaggcg ggcgaggcgt ccgacgtggg tgtccaccac
     9901 gacgccgtcg ttgatggcga aggcattgcc gagcaccacg ttcgcggtct tccggccgac
     9961 gccgggaagg gcgtggagtt ccgccatcgt ccgggggacc cggccgccgt gctccgccac
    10021 cagcgcctgt gccatggcga tcaagttctt cgccttgttg cggaagaagc cggtggatcg
    10081 gatgagccgt tcgacatcgt cctggcgcgc gccggcgagc gcggacggcg tgggatacgc
    10141 gcggaagagc tcgggcgtga ccaggttgac ccgcacgtcg gtgcactgcg ccgagaggat
    10201 cgtggctgcg aggagttggt aggcgttccg gtggtccagt gcgcagtgcg cgtccggata
    10261 cgcgcgatgc aggaggctga acaccctggc cgggggggca ggccggcgcg tcttcgcctt
    10321 cacgcgcccc gccgcttccg cgcgaaggcc aggaactcct cgacgctccg ggtgttgagg
    10381 atatccgctg cggtgagcca ccccttccgg gcgatcccga cgccccattc catgttcccg
    10441 atgccgccga cgtcgtgggc atcggcgccg atcgagatgg tgaggcccct ggccctcgcc
    10501 gagcggagca cccgccagtc gaggtcgagg cggtgggggt cggcgttgat ctcgatcgcg
    10561 gcgccgacgg cggccgcctt ttcgatgagg gcgtcgaggt cgaccgcgta cgcttcccgg
    10621 gccaggagga gccggccggt cgggtggccc acgatggtga ggtgcgggtc gtcgagggcg
    10681 cggagcatcc gggccgtcat ctcgtcccgc cccatctgga agcggctgtg gaccgagccg
    10741 atgacgaagt cgaggcggtc gagcacggcg gcgggatagt cgagcgcgcc atcggccagg
    10801 atgtcggcct cgatcccctt gagcacccgg acgccggtga ggcgcgcgtt gaccgcgtcg
    10861 atctcgtccg cctggcgccg cagcgcatcg ggcttgagcc cgccggcgta ggccgccgcc
    10921 tggctgtggt cggtgagccc gacgtacgcg tagccggcgg cctggcaggc gcgcgccagg
    10981 tcctcgatcg gggtggtacc gtccgaccag ttcgagtggc agtggaggaa gccgcggagg
    11041 tcggcgcggg tgacgagcgt cggaagcacg ccgtcccgcg cggcggcgat ctcgtcggtg
    11101 ccttcgcgga gttcgggcgg ggtctcctcg agcccgagcg cgcgatagaa cgtggcctca
    11161 tcgggggtgg gcacgaacgt gctgccgtgc cagagcgccg cgccgtcgag cgtgtggccg
    11221 gtcgcgcggg cgtgcgcctc cagcgccgcg acgtgggcgt cgctcccggt ggcgcggacg
    11281 agcaccgcgc ccgcgttctt cagcgtggtc acgatgacct gcgccaccga cccgccgccg
    11341 tggcggagcg tcacccgccg ttcgtcgtgc ccggagaact cgacgatgcc gggaatggcg
    11401 gcgaggcgcc tgaagaattc ggcgggcggc gtgtcgccga tgaccacgaa gctgaggccg
    11461 ttgaccacct ccatccggcg ccggacgtct cccgcggtga ccacccgctc cacgcccggg
    11521 agcgccgcga gcgtggccgc gagcgaggcg gcctcggcgg cggcgtggtg gcagagccgg
    11581 aacgcgctcg accggcggag gaactcgatg ctcgccagga tgttggcggc ggtcttggca
    11641 ccgaagcgcg gcagcgcggc cagggagccg tcctgcgcgg cggcctcgag ctccgcgatg
    11701 gtctcgatgc ggagggtctc gtggatctgg cgcaccttgg caacgcccag gcccgggagc
    11761 tgcagcatct caaggaggcc cggcgccacc tgtccccgca gctcctcgag cagggcgctc
    11821 ctgccggtgg cgacgagctc ctcgatcagc tgccgggtgg cggggccgat gcccttggcc
    11881 gacccgagcg agccgtcggc gatcgcctcc gccacggtgc cggggagggc cgtcacggcg
    11941 cgggccgcgc cgcggaaggc gcggacgcgg aaggcgttct cgcccttgag ctccaggagc
    12001 gccgccactt gctccagcgt ctccacgacg ccgtccttgt cgatggcgct cacggcgcct
    12061 ccccgccggc ccgaaaggcg agctcgagga gctcatccgc cccgaccagc gagtgaaggc
    12121 cggcggcgtc gccgccgggg agcgccggcg agaatggagg cgaaccgtcc gcgcggcgaa
    12181 gtgacagcgg ccgtggtccc gccacctccg cgacgccgaa gccggggcgg ccggggtgga
    12241 cgccgaagac gccgagcacc tcgatcgcgg cggcaggtgc gggtcggacg aagaagccgt
    12301 cgaggggctc gtgcggggcg tccggactga gctgggccca gacgaggcgc tcggggaact
    12361 gatggtaggc ggacgggcgc acggtcaggg cggtgtccgg ccaggcgggc gagaggagcg
    12421 tcgttgcacg ggaccggtcg acggccacca cggggcgccc gttccgccag aagaggtatg
    12481 cgtggtgcag caccgccacg aactcctcga tctggtcgac gtcgtgttcc tcgggcacga
    12541 ggtcctggag gagctcgacg accgcgcgat cgaggaggaa ggcatcccga tcggcctcgt
    12601 cgcggcctgc ggccaggagc gaggcggcga gcgcggggaa ccggtcgtcg gccatctggc
    12661 cgaagacgag cgtgaagggg tcggggcgct cagccacggc cggtccgggg gaggaaggcg
    12721 tcgagcaccc ggcggaaggt ctcgaaccct tcgatgtacg ggacgtggcc gcagtcgggg
    12781 atcaggtgga actccgcgcc gaggcagtcg gcggtctccc gggccgactc ccaggggatc
    12841 acgtcgtcgg cgccgtggag gacgaccgtc gggatctcga gcttccggag cgcggggcgg
    12901 aggtcgtact cgccgaggga ggcccagacc tccttctgga cccgctcgac caggcggaag
    12961 ccggagagct ctgcgacgcg cgagggatcc ttgaagtagg cgatgacggc gagttcgaag
    13021 agccggcgct ggtaggcggc gagatcgctc gactggaggc cggactcccg gagccgccgc
    13081 cgctcggcct gggcccattc gccgttggtg atcctggcgt agcgccgctc gtactcgagc
    13141 cgcgcctcgc gatacgacgg ggcgggggag acgagcgcca gccgggccac gctgcagggg
    13201 tgccggatgg cgtagagcat cgcgaggagg ccgccccagg agtagccgat caggtcgtgg
    13261 ggctggtcgc cccagagttc cttgagggcg tggaggtcgg caacgtgctc ctcccatccc
    13321 gccttgatct cccgcggcag cggcgagcgt ccgccgcccc gctgatcgta gagcacgagc
    13381 gtgcgtccct cggccagcgc gtcgaagccg ggcaggagat agtcgtggct ggcgccgggg
    13441 ccgccgtgga gcgccacgac cgcggggcct gagccgctgc ggcgctcgaa gatgtggatg
    13501 ccgttgacgt gccgggtgaa ggtctcgacc ggcgaggtga tcggggtcat ggtggggatg
    13561 gaatgtaccc gtgtcgcggg tgacgggtga cggttggcga gtgggtgacg aggcgcgggt
    13621 cggcgggatg ggccaccctg tcgccgggcg accaccccca acccgccact cggtacccgg
    13681 cacgcatccg ccacccgcaa ctcgccacct gccacttgcc ccttgccact ccctactgac
    13741 cctcgatttc gcccgctaga ttgcgcccac catgaccgcg cccgccgagt ccgaac
//
LOCUS       DASUAP010000232         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_892213, whole genome shotgun sequence.
ACCESSION   DASUAP010000232 DASUAP010000000
VERSION     DASUAP010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892213"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..714)
                     /locus_tag="VFU45_06025"
     CDS             complement(55..714)
                     /locus_tag="VFU45_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU5358653.1"
                     /translation="MPERPYGIIAPAVLRLFRGLKRLPPQSFVGDRLSHQMGEYFTAD
                     PESATRRRAWRVDIFVLASVAVELIVFFTIFGAPEWLQVVLLAMPILRILNIIEVTVN
                     LNVFDALTAEVKPRVASVVRTLVLTGINYVELILCFGIIYAVALADLHGAHDPLDVVY
                     FSAITQLSVGYGDIVPMHLLRLAAVAQAMLGLFFTVVLLGRFAGLLPRIKTVAEMEDE
                     K"
     gene            complement(972..1424)
                     /locus_tag="VFU45_06030"
     CDS             complement(972..1424)
                     /locus_tag="VFU45_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5358654.1"
                     /translation="MQPDSAVILLVEDDANDVVLITRAFTKAGAFGVIEAVGDGDAAL
                     AYLRGEGRYADRQRYPFPDVVLLDLKLPRRSGLEVLEWIRGQHPLRRLPTVMLTSSRD
                     ATDVDRAYDLGANSYLVKPVGFDELQRLVAEVSRYWLEHNQGPDAQSA"
     gene            complement(1414..>2691)
                     /locus_tag="VFU45_06035"
     CDS             complement(1414..>2691)
                     /locus_tag="VFU45_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5358655.1"
                     /translation="VPKAVYVQWFRLRSSPQARAQLNALIGPGARLSIGGTEGKWTDL
                     GQAVPAPPVPIDTATGVLQYDRPGTGPVVAMARRVPGTPWSVLVEFTRAAALGGAVRF
                     LQRLALITGILLIVLLAGTWIAVGRLVRPLAALTSAAEAVTQGRLDGRVGGERRNDEI
                     GRLADAFDAMVARVREAREGLERRVAERTRELKERNEELEAFGYSLAHDLRAPLRAMQ
                     GFSQALLEDHSSQLDPTGRHYAELVSDGARTMDRMILDLLNYSRIARAELQPAPVELT
                     SVIGAATRQLKRDLTDRHSRISVAPELPPVLAHEATLVQVFANLIGNASKFVPPDRTP
                     EIRISSIPWNGAVRVWVEDNGIGIPPQFQQKIFGVFERLNGADEYPGTGIGLAIVRKA
                     MEKMGGAVGVESTPGTGSRFWLELPALEVKGAA"
BASE COUNT          425 a          850 c         1019 g          397 t
ORIGIN      
        1 acttcttact tctcacttct tacttctcac ttcttacttc tcacttctta cttcttactt
       61 ctcatcctcc atctccgcca ccgtcttgat gcggggcagg aggcccgcga agcggccgag
      121 caggacgacg gtgaagaaga ggccgagcat cgcctgggcc acggcggcga ggcggaggag
      181 gtgcatcggg acgatgtcgc cgtagccgac ggagagctgg gtgatggcgc tgaagtagac
      241 gacgtcgagc gggtcgtggg cgccgtggag gtcggcgagg gccaccgcgt agatgatgcc
      301 gaagcagagg atcagttcga cgtagttgat gccggtgagg accagggtcc gcaccaccga
      361 cgccacccgc ggcttgacct ccgcagtgag ggcgtcgaag acgttcaggt tgaccgtgac
      421 ctcgatgatg ttgaggatgc ggaggatcgg catggccagg agcaccacct ggagccactc
      481 cggcgccccg aagatcgtga agaagacgat gagctcgacg gccaccgacg cgaggacgaa
      541 gatgtccacc cgccaggcgc ggcggcgggt ggccgactcc gggtcggcgg tgaagtactc
      601 tcccatctgg tgggagaggc ggtcgccgac gaaggactgg ggcggcaggc gcttgagccc
      661 gcggaagagg cggagcaccg cgggagcgat gatcccgtag gggcgttcgg gcatggggga
      721 aaataagcgg gggaaagcgg gggaaagcga gggaaggcgg gggaaggtgg gagaaaagag
      781 gcgagtggaa cgtggcgggt gggaactggg gtggcgcggc ccgaacggag tgtcatcccg
      841 agggagcgaa gcgaccgagg gaccttccct gggccgatcg acgatggcga caggtcccga
      901 tccgagcggg ccaggtacct cgcctccgtt cgggatgacg ttccttgtgc accgggcccc
      961 gtgtgccgag ctcaggcgct ctgtgcgtcg ggcccctggt tgtgttcgag ccagtagcgg
     1021 ctgacttcag cgaccaggcg ctgcagttca tcgaagccga cgggcttgac gaggtaggaa
     1081 ttggcgccga ggtcgtaggc gcggtccacg tcggtggcgt cgcgggagga ggtgagcatc
     1141 accgtcggga gccggcgcag cgggtgctgg ccgcggatcc attccaggac ctcgaggccg
     1201 ctgcgtcgcg ggagcttgag gtcgaggagg acgacgtcgg ggaacgggta ccgttgccgg
     1261 tcggcgtagc ggccctcccc ccgcaggtag gccagggcgg cgtcgccgtc gccgacggcc
     1321 tcgatgacgc cgaaggcgcc ggccttggtg aaggcgcgcg tgatgaggac cacgtcgttg
     1381 gcgtcgtcct cgaccaggag gatcaccgcg ctgtcaggct gcacccttga cctccaatgc
     1441 gggaagttcg agccagaacc ggctgccggt gccgggggtg gactcgacgc cgacggcgcc
     1501 gcccatcttt tccatcgcct tccgcacgat ggcaagcccg atgccggtgc cgggatattc
     1561 atcggcgccg ttgaggcgct cgaagacccc gaagatcttc tgctggaact gcggcgggat
     1621 gccaatcccg ttgtcttcca cccacacgcg aacggcaccg ttccacggga tggaactgat
     1681 gcggatctcg ggggtccggt cggggggcac gaacttcgac gcgttcccga tcaggttggc
     1741 gaacacctgc accagcgtgg cctcgtgggc gagcacggga gggagctccg gcgcgacgct
     1801 gatccgcgag tggcggtccg tcaggtcgcg cttgagctgg cgtgtggcgg cgccgatgac
     1861 ggacgtcagc tcgacgggcg cgggctgcag ttccgcgcgg gcgatgcggc tgtagttgag
     1921 gaggtcgagg atcatccggt ccatcgtccg cgcgccgtcc gacacgagct ccgcgtagtg
     1981 gcggccggtc gggtcgagct gggaggagtg atcctccagg agcgcctggc tgaacccctg
     2041 catcgcacgc agcggggcgc ggaggtcgtg ggccagggag tagccgaacg cctcgagctc
     2101 ctcgttccgt tccttgagtt cgcgggtgcg ttccgcgacg cgccgctcca gcccctcccg
     2161 cgcctcccgc acccgcgcca ccatcgcgtc gaacgcgtcg gccaggcggc cgatctcgtc
     2221 gttcctccgc tcgccgccga cccggccgtc gagccgcccc tgggtcacgg cttccgcggc
     2281 gctggtgagc gcggccagcg gacggaccag gcggccgacg gcgatccagg tcccggcgag
     2341 cagcacgatg agcaggatgc cggtgatgag ggccagccgc tggaggaacc ggaccgcgcc
     2401 cccgagcgcg gcggcacggg tgaactcgac cagcaccgac catggcgtgc cggggacgcg
     2461 ccgggccatc gccaccacgg gtccggtgcc gggccggtcg tactggagca cgccggtcgc
     2521 ggtgtcgatc ggcacgggag gcgcggggac cgcctggccc aggtcggtcc acttgccctc
     2581 cgtgccgccg atcgagagcc gggcgccggg gccgatgagc gcattgagct gggcccgcgc
     2641 ctggggcgac gatcgcagcc ggaaccactg cacgtagacc gccttcggca c
//
LOCUS       DASUAP010000233         5356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_865035, whole genome shotgun sequence.
ACCESSION   DASUAP010000233 DASUAP010000000
VERSION     DASUAP010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865035"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFU45_06040"
     CDS             <1..183
                     /locus_tag="VFU45_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HEU5358656.1"
                     /translation="VDGIVERIAREWPGGKRPKVVATGGLAALVAPLCRTIEVTTPDL
                     TLHGLRIAARHLRVGA"
     gene            186..1100
                     /locus_tag="VFU45_06045"
     CDS             186..1100
                     /locus_tag="VFU45_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEU5358657.1"
                     /translation="MAAPERHRWAYAAFGRAAPYLLHLRPVEWPIMAAHTFLGYLLAV
                     GLAGVAAGRSLPAAGLGLLLWVVCLNGGTLAINSAFDNDEGDVAYLRAPPKPPRHLFA
                     WGLGLMLLGQALAFELPRPFVLAYAACFLMSVLYSVPPFRLKAVAGADLVINMWGFGT
                     FTPFAGWATTGRTLDLPHALVLLGFCPLFAALYPLTQIYQVEEDTRRGDRTLVIRLGF
                     GRSLGVAIGATLLAFALFGWSLAIVGGGTPLKALGLALALTAWLGVLVPWARRHPSMT
                     AALHQRGMYLALGAWAITDVVVALGWGT"
     gene            1230..1859
                     /locus_tag="VFU45_06050"
     CDS             1230..1859
                     /locus_tag="VFU45_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358658.1"
                     /translation="MNAVAQASMTVDHTDVRYLVRAAVKIGFLTAVTVLVFSLVSRFT
                     DGTVMTALETVILLAGLSGVVLLPGRWTNPRTVEGIAGAAAIGLGGTFIFLLLDVSIL
                     QNIGTYTDRWREIGGGSNWWWHPVWWMLGTYLSWFGAFAQANQAVRRGAAQPAMVMIL
                     SVVYALVLGALANAIHFPGARWTMPTFAIAFIPGLALTVLTTALSPKRA"
     gene            1866..2159
                     /locus_tag="VFU45_06055"
     CDS             1866..2159
                     /locus_tag="VFU45_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358659.1"
                     /translation="MYAIRLLLKGLGWLLTPLVASAASFFGAELGTLLSEWVTSPVAG
                     LVLTVACGAATGFVGTHFWIRYLQRRPRLSRALQIDPSMLPPLDGPPSGGPAE"
     gene            2156..2539
                     /locus_tag="VFU45_06060"
     CDS             2156..2539
                     /locus_tag="VFU45_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358660.1"
                     /translation="MIRWVAASLLLATACGRGAPAPSRPAPPPRAPAPPDSLALSIAG
                     GTGVWFTFARADTAADGRTCLERTLEIRHADGRRVAVPLLYTGAPPVLLNDSTIRADI
                     WVHCAPTERYRIDLGSGIPTAERRR"
     gene            2536..3504
                     /locus_tag="VFU45_06065"
     CDS             2536..3504
                     /locus_tag="VFU45_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU5358661.1"
                     /translation="MIGRAAGVAATCCALSAAALPAQAVSGDGGYVLTSTTRQTWRIG
                     FSRELLGPTRLRLYGTRMWHAGGGPDLYGLGADLTAFEGGSPGPYLVGGFSGGVARNA
                     GSDWWNSWSAGVGYEVVPFRWLSLRGEGRYRVINPDHHTGLEFSLGLGLHFGVGRPPD
                     QPAYTSVRHATPPGSPEVVSMAEKKGFSGDKAELVAGVVATATSAMGTPYTWGGTGKN
                     GDGFDCSGLIQWAYAQHGIRLPRVSRDQAREGTKVPKRLDALAPGDILTFSNSGTFVT
                     HVALYVGDGKIIHSANGGVQMSVLSEDDPYGRWWWRRWVGARRIVN"
     gene            complement(3608..4651)
                     /locus_tag="VFU45_06070"
     CDS             complement(3608..4651)
                     /locus_tag="VFU45_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358662.1"
                     /translation="MLAPLLLAFAALQAPAPGTVTVTVDSAHHDVVVTAGPYDLPQAM
                     AGMSHAEMEHMTESQTELKRFAWPVDGWFRGFALEVRDGNGKALSRRILHHMIMINFD
                     RRQLYYPAYERLMGAGQETADVTIPATIGVPMRSGMHLGMYLMWHNETGADIHGAYLV
                     LRLHYAPKNQLPRPVDAMPIYMDVNLTVGGDNVFDLPPGITKKSHDFVQPIDGHLIAA
                     GGHLHDFGMFVRLADVASGKTLVQLNAERQPNGDVTGLPRKLFGVSGAGLRLKAGRTY
                     RVTGEYDNLTHDLIKDGAMAHIVGLFTPDDMRQWPKLDVNDPGTRADLADLESLGKAS
                     GGMRMGTMNHMKD"
     gene            complement(4709..>5356)
                     /locus_tag="VFU45_06075"
     CDS             complement(4709..>5356)
                     /locus_tag="VFU45_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358663.1"
                     /translation="MGFVIMWDDRAGAPIRGVRARLTLEWTPANQLPRPVDVLPVYLD
                     VRDPVGRSAAFDLPAGRSTFHADYTLPTSGRIIAVGGHLHDYGIGISLDDITSGTPRN
                     VVRLGTRLDPSGAIESVDRRYPGIAGAGIHLAAGHRYRITGSYDNPTGRTIPDGAMVH
                     LIYLFTPDRTDDWPSMASADSGLAADLAWLDRPAPAEHVPDARTPGHAHTAGEHR"
BASE COUNT          675 a         1888 c         1909 g          884 t
ORIGIN      
        1 gtcgatggga tcgtcgagcg catcgcgcgg gagtggcccg gcggtaagcg cccgaaggtg
       61 gtcgccacgg gcgggctcgc cgccttggtg gcgccgctct gccggacgat cgaggtgacg
      121 acgccggacc tgacgctcca cgggctccgg atcgcagcgc ggcacctgcg ggtcggcgcg
      181 tagcggtggc ggcacccgag cgccaccgct gggcctacgc cgcctttggc agggcggcgc
      241 cctacctcct ccacctgcgg ccggtcgagt ggcccatcat ggccgcgcac accttcctcg
      301 gctacctgct cgcggtgggc ctcgcggggg tggcagcggg gaggtcgctc ccggcggccg
      361 ggctcgggct cctgctctgg gtggtgtgcc tcaacggcgg cacgctcgcc atcaattccg
      421 ctttcgacaa cgacgagggt gatgtcgcct accttcgggc gccccccaag ccgccccggc
      481 acctctttgc ctgggggctt ggcctgatgc tcctggggca ggcgctcgcg ttcgagctgc
      541 cgaggccgtt cgtgctcgcg tatgcggcgt gcttcctgat gtcggtgctt tactccgtgc
      601 cgccctttcg cctcaaggcc gtggccggtg ccgacttggt catcaacatg tggggtttcg
      661 ggaccttcac gccctttgcg gggtgggcca cgaccgggcg cacgctcgac ttgccgcacg
      721 cgctggtgct gctgggcttc tgtcccctct tcgccgcgct ctatcccctc acccagatct
      781 accaggtgga ggaggacacc cgtcgcggcg accggaccct cgtcatccgg ctgggcttcg
      841 ggcggagcct tggcgtcgcg atcggcgcca cgctgctcgc gttcgcgctc ttcggctggt
      901 cgctggcgat cgtcggcggg ggcactccgc tcaaggcgct cggactggcg ctggcgctca
      961 ccgcctggct cggcgtgctc gtgccgtggg cccgccggca cccgtcgatg accgcggcgc
     1021 tccaccagcg ggggatgtac ctggcgctcg gcgcctgggc gatcacggac gtggtggtgg
     1081 cgctggggtg ggggacgtag actgcgaacg tgcggggtgc gtgggggcgg cggactcgcc
     1141 cgcgtacctc gcacgtcgca cctcctagta ccacccggca cccgccccag ctatactaga
     1201 cccacaccac aaccccatcc ggagcagcca tgaacgcggt ggcccaagcg tccatgacgg
     1261 tcgaccacac cgacgtccgc tacctggtcc gcgccgcggt caagatcggt ttcctgaccg
     1321 cagtgacggt gctcgtcttc tcgctcgtct cccgcttcac cgacggcacc gtgatgaccg
     1381 cccttgagac ggtcatcctg ctcgctggcc tctccggggt ggtgctcctc ccgggccgct
     1441 ggaccaaccc gcgcaccgtc gaggggatcg ccggcgccgc cgccatcggc ctcggcggca
     1501 cattcatctt cctgctcctc gacgtctcca tcctgcagaa catcggcacc tatactgacc
     1561 gctggcgcga gatcggtggc ggctccaact ggtggtggca cccggtctgg tggatgctcg
     1621 gcacctacct gagctggttc ggcgcctttg cccaggccaa tcaggcggtg cggcggggag
     1681 cggcccagcc ggccatggtg atgatcctgt cggtcgtgta cgcgctcgtc ctcggcgccc
     1741 tggccaatgc catccacttc ccgggcgccc gctggacgat gccgacgttc gccatcgcgt
     1801 tcatccccgg cctcgcgctc acggtgctca ccaccgccct cagcccgaag cgcgcctgag
     1861 ggcggatgta cgcgatccgg ctcctgctga aggggctcgg ctggctgctg acgccgctgg
     1921 tggcgtcggc ggcctctttt tttggggccg agctcggcac gctcctctcg gagtgggtga
     1981 cgagcccggt ggcggggctg gtgctcacgg tggcctgtgg cgccgccacc ggcttcgtcg
     2041 gcacccactt ctggatccgc tacctccagc gccgcccgcg cctgtcacgc gcgctgcaga
     2101 tcgacccttc gatgctgccg ccgctcgacg ggcctccatc cgggggcccg gccgagtgat
     2161 ccggtgggtc gcggcctcgc tgctgctggc caccgcctgc ggccgggggg cgccggcccc
     2221 gtcgcgtcca gcgccgccgc ccagggcccc ggcgcctccc gattcgctgg ccctcagtat
     2281 tgcgggcggc accggggtgt ggttcacctt tgcccgggcc gacaccgccg ccgacgggcg
     2341 gacgtgcctg gagcggaccc tcgagatccg gcacgccgat ggtcgccgcg tcgccgtccc
     2401 gctcctctac accggcgccc cgccggtgct gctcaacgac tccaccatcc gcgccgacat
     2461 ctgggtgcat tgcgcgccga ccgagcgcta ccgcatcgac ctgggttccg gcatccccac
     2521 ggcggagcgg cggcggtgat cggacgcgcg gccggcgtgg ccgcgacgtg ttgcgcactg
     2581 tccgcggccg cgctgcccgc gcaggccgtc tccggcgatg gcggctacgt cctcacctcc
     2641 accacccgcc agacctggcg catcggattc tcccgcgagc tcctcgggcc cacccgcctc
     2701 cggctctacg gcaccaggat gtggcacgcc ggcggcggcc ccgacctcta cggcctgggc
     2761 gccgacctga cggcgttcga gggggggagc ccggggccgt acctcgtcgg gggcttcagc
     2821 ggaggggtgg ccaggaacgc ggggagcgat tggtggaaca gctggtcggc cggcgtcggg
     2881 tacgaggtgg tccccttccg gtggctgtcg ctccgcggcg agggccgcta ccgggtgatc
     2941 aaccccgacc accacaccgg gctcgagttc tccctcggcc tcggcctgca tttcggggtg
     3001 gggcggccac cggaccagcc ggcatacacc agcgtccggc acgccacgcc ccccgggtca
     3061 cccgaagtgg tgtcgatggc ggagaagaag ggcttctccg gcgacaaggc cgaactcgtg
     3121 gccggcgtcg tcgccacggc gaccagtgcc atgggcacgc cgtacacctg gggcggcacc
     3181 gggaagaacg gcgacggctt cgactgctcg gggctcatcc agtgggccta tgcccagcac
     3241 gggatccggc tcccccgcgt gagccgcgac caggcgcggg aggggacgaa ggtacccaag
     3301 cggctggacg cgctcgcgcc cggggacatc ctgacttttt cgaacagcgg cacgttcgtg
     3361 acccacgtgg cgctctacgt cggcgacggc aagatcatcc acagcgccaa cggcggcgtg
     3421 cagatgagcg tgctgagcga ggacgacccg tacgggcgct ggtggtggcg gcggtgggtg
     3481 ggcgcgagga ggatcgtgaa ttagtcgcgg agggagaagt aaagagtgaa gagtgaaaag
     3541 gtgctccacg ctcagtgggc ctccctttca ctcttcactt ttcacttgtc actcttcact
     3601 tcttcgttca gtccttcatg tgattcatcg tgcccatccg catcccgccg ctggccttgc
     3661 cgagggattc gaggtcggcc aggtctgccc tggtgcccgg gtcgttgacg tcgagcttgg
     3721 gccattgccg catgtcatcg ggagtgaaga gcccgacgat gtgcgccatc gcgccgtcct
     3781 tgatcaggtc gtgcgtgagg ttgtcgtact cgccggtcac gcggtaggtg cgccccgcct
     3841 tgagccggag gccggcgccg ctcacgccga agagcttgcg ggggagcccg gtgacatcgc
     3901 cgttgggctg ccgctcggcg ttgagctgca ccagcgtctt ccccgacgcc acgtcggcca
     3961 gccggacgaa catcccgaaa tcgtggaggt gcccacccgc cgcgatcagg tgcccgtcga
     4021 tcggctggac gaagtcgtgg ctcttcttcg tgatgccggg gggcaggtcg aacacgttat
     4081 cgccgccgac ggtcaggttc acgtccatgt agatgggcat cgcgtcgacg gggcggggga
     4141 gctggttctt cggcgcgtaa tggaggcgga gcaccaggta agcgccgtgg atgtccgcgc
     4201 cggtctcgtt gtgccacatc aggtacatcc cgaggtgcat ccccgaccgc atcggcacgc
     4261 cgatcgtcgc ggggatcgtc acgtcggcgg tctcctggcc cgcgcccatc agccgctcgt
     4321 aggccgggta gtagagctgg cgccggtcga agttgatcat gatcatgtgg tgcaggatcc
     4381 gccgggagag cgccttcccg ttcccgtcgc gcacctcgag ggcaaaaccg cggaaccagc
     4441 cgtcgaccgg ccacgcgaag cgcttgagct cggtctgcga ctcggtcata tgctccatct
     4501 cggcgtggct catcccggcc atcgcctggg ggaggtcgta cggcccggcg gtcaccacga
     4561 cgtcgtggtg ggccgagtcg accgtgacgg tcaccgtgcc gggggcgggg gcctgcaggg
     4621 ccgcgaaggc gagcagcaat ggtgcgagca tgggagtctg tggatcctgg tacaggtgaa
     4681 ggtgcggggc ggggccgttg cccgcccgtc agcgatgttc gccggcggtg tgcgcgtgtc
     4741 ccggcgtgcg tgcgtccgga acatgttcgg ccggcgccgg ccggtcgagc cacgccaggt
     4801 cggccgccag gccggagtcc gcgctggcca tcgacggcca gtcatcggtc cggtccgggg
     4861 tgaaaagata aatgaggtgg accatcgctc catccgggat ggtccggccg gtcggattgt
     4921 cgtagctgcc ggtgatccgg taccggtgcc cggcggcgag gtggatcccg gccccggcga
     4981 tgccgggata cctccggtcg accgactcga tggccccgct cgggtcgagc cgggtgccga
     5041 ggcgcaccac gttccggggg gtgccggagg tgatgtcgtc gagcgagatg ccgatgccgt
     5101 agtcgtggag gtggccgccg accgcgatga tccggccgct cgtcggcagc gtgtagtcgg
     5161 cgtgaaaggt cgagcgcccc gccggcaggt cgaacgccgc gctccgcccg accgggtcgc
     5221 ggacgtcgag gtacacgggg agcacgtcca ccggccgcgg cagctggttg gccggggtcc
     5281 actcgagcgt gagccgggcc cggaccccgc ggatcggcgc gccggcccgg tcgtcccaca
     5341 tgatcacgaa gcccat
//
LOCUS       DASUAP010000234         6925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1233408, whole genome shotgun sequence.
ACCESSION   DASUAP010000234 DASUAP010000000
VERSION     DASUAP010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233408"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..931
                     /locus_tag="VFU45_06080"
     CDS             164..931
                     /locus_tag="VFU45_06080"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU5358664.1"
                     /translation="MIPLAILAALQAAPHAPAIPRDTTVRTLASYPFQVGEEYGYTAR
                     LGVLTIGSASMAIPRIDTIRNAPSFVFRFTLNGRALFFTVDDTLTSWTGIADFKSRRF
                     LQKNMEDGSLWLRDRAIFADSGYFRTLGDSSHSPTPDVPLDDAAFFYFVRTIPLEVGQ
                     TYTFSRYFVKDQNPITVRVLKRDGCDLPDGRHLQCLVLAPVVKAKGMFSAEAQARVYL
                     TDDRLRIPVEIQSRFPFGVIILKLHDMKVPPIGPVAG"
     gene            934..1887
                     /locus_tag="VFU45_06085"
     CDS             934..1887
                     /locus_tag="VFU45_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358665.1"
                     /translation="MAYREADLSKVRTVPVGTRRNKVEGALLAHPPAADRSFHAFLDS
                     LPDILAARDLRTVIQHVADAARRRKGVVLLLGGHVMKVGMGPLIRAWLDAGIVSHIAM
                     NGAAAIHDFELAAFGGTSEDVEAGLGDGTFGMAEETGAEMNAAIALAASRGRGLGEGL
                     ADALAQRDTLPGREVSVLLGCRQHDVPVTVHAAIGAEIIHQHPTTDGAALGATSHRDF
                     RRLAGSLPDLDGGGAVLNWGSAVVMPEVFLKALTIARNIGGGRPRDFLAADFDMQRHY
                     RPRVNVVQRPTKAGGIGIQLTGHHELLLPMLMWGVLEKLGA"
     gene            complement(2053..2262)
                     /locus_tag="VFU45_06090"
     CDS             complement(2053..2262)
                     /locus_tag="VFU45_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358666.1"
                     /translation="MFRSLLGYAVLAVVAYFALQIVLGLFGIVVGLVFKLLWLAAIGF
                     IVYLVIRVLSPKTADKIADTMKGKS"
     gene            2359..3441
                     /gene="thiO"
                     /locus_tag="VFU45_06095"
     CDS             2359..3441
                     /gene="thiO"
                     /locus_tag="VFU45_06095"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEU5358667.1"
                     /translation="MIGSGAIGAACGRELAVAGRRVLLLDRAPQGGEAWQAAAGMLAP
                     QIEAGPDDPIFQLAVAARERYVELAPALQEGVGIDIGLWLEGIARVAVSAEDVPELRA
                     RAAWQRQQGHLCDWFDPTEVKARYPWMGANHGALWAPRDGALDPTALVRALRADAVRL
                     GARQEIVERVEILRDGDRVIGVHGGRKFSSEHVVVAAGAWSADIGGLPRPVSVEPVRG
                     QMAALPWPAGIDPAIVFGGTAYVVARGGEAVVGSTMEYAGFDATTTAAGLARIFAGVS
                     AICPPLAGLEVKRTWAGLRPMTPDGLPIIGAEPRARGLWYATGHGRNGILLAAMTGTV
                     ISDLIAGKAATVEGLTAVSPERFWRW"
     gene            3593..4813
                     /locus_tag="VFU45_06100"
     CDS             3593..4813
                     /locus_tag="VFU45_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358668.1"
                     /translation="MPLQQWAGESVHVRSAAFVYLTDGSYHILAGSDTLAVTRIDDST
                     VAVAPPDAGSRTVAFTITGAGATAHMGSVQFFGFGGIREPDAGFGGDYEAWPAGTIGA
                     TGVLGWVNDSGTGYSGSIATLDLRTGHVTTHPGLGYHGSYGPAPGYLPDVVTTSLDVS
                     PVAAVHLGNPHDTLVSIAIPGFPRTDYLIGPFVEFHTQSHSAYARTSTDSGRTWHTVK
                     DTLYLNSTWQLVLSPTKRSATVINGWSDSGGVVLDATSGLTRYRLTRMREVDAAAFSA
                     DGTVLYVAGSSTGPGIAQSLEAVDAATGAALHVDSLLGGTAISAVVDPAGPQLFVAVA
                     RPDGTLALNVYDATTLRRTATLHSTATGFDLTQFIQSGASMADRAVYVARSGGMIARF
                     DILPRAGQPAPGRR"
     gene            4974..5579
                     /locus_tag="VFU45_06105"
     CDS             4974..5579
                     /locus_tag="VFU45_06105"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5358669.1"
                     /translation="MTKPAPIPPSTVDDQALIQRVASGDEQAIGQLYDRYASVLHAAA
                     WRITGETADAEEVLLDAFAQAWRDASRYDAQRGSVGAWLMTITRSRALDLIRSRGRRE
                     RAATAAGADPEAAGIGASTWRYDTSRSAEQSERRRLISIALQSLSAAQRQAIELAYYE
                     GLSHSEIAAKLGEPLGTVKTRVRLGLQKLREALRPYYGTAA"
     gene            5576..6463
                     /locus_tag="VFU45_06110"
     CDS             5576..6463
                     /locus_tag="VFU45_06110"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU5358670.1"
                     /translation="MNERESLQDLAADYALGLLGPEETAAFELELAQSPGLQLIVREC
                     REALAVLPGGIQSPAPAADLRARVIARALGTPADPAARDATDDIGSAAGTDTGPAADD
                     DVVLPITSESPVARAPRRHGSSAGWWMALAASLIAATGLGVLWRSALRAGQAALVREA
                     ALRASVDSVSKQLAARDATLRAILAPGVELHLLRSTGAPEPGIEFFWNKKSHSALMYA
                     FNLTPSAKGRTYQLWMIQGDKRIPSVTFEAGSDGHALVDKIAMPPDLPFEALAVTDEP
                     AGGSAQPTTPVLLVASIGS"
     gene            6769..>6925
                     /locus_tag="VFU45_06115"
     CDS             6769..>6925
                     /locus_tag="VFU45_06115"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727449.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU5358671.1"
                     /translation="MPAPPALERKPGWLKVKAPGGPSYLDVKRMMRELDLHTVCEEAR
                     CPNIGECW"
BASE COUNT         1044 a         2527 c         2329 g         1025 t
ORIGIN      
        1 gcgtggacac cgtcgccgcc acagaacgtc acgacctgcg ccagcgcccc tcccggctcg
       61 acccctgggc cgaggcgcgc gcgctctggc accatcggcc ggtgctgcgc gaccttccgc
      121 cactcgaacg gcgtggcccc cgacttgcag aggaagcgcc ggcatgatcc cgctcgccat
      181 cctcgccgcg ctccaggccg cgccgcacgc gccggccatc ccacgggaca ccacggtccg
      241 caccctggcc agctatccct tccaggtcgg cgaggagtac ggatacaccg cgcgactggg
      301 cgtgctcacc atcggcagcg cgtcgatggc catcccccgg atcgacacca tccggaatgc
      361 cccgtcgttc gtcttccgct tcacgctcaa cggccgcgcg ctcttcttca ccgtcgacga
      421 cacgctgacc tcgtggaccg gcatcgccga cttcaagagc cgccggttcc tccagaagaa
      481 catggaggac ggctcgctct ggctgcgcga ccgcgccatc ttcgccgatt ccgggtactt
      541 ccgcaccctg ggcgactcct cccacagccc caccccggac gtgccgctcg acgacgcggc
      601 gttcttctat ttcgtgcgca cgatcccgct cgaggtggga cagacctaca cgttctcccg
      661 gtacttcgtg aaggaccaga atcccatcac cgtccgggtg ctcaagcgcg acggctgcga
      721 cctccccgac ggtcgccacc tccagtgcct ggtcctcgcc ccggtggtca aggccaaggg
      781 gatgttctcg gcggaggccc aggcgcgggt ctacctcacc gacgaccggc tccggatccc
      841 ggtcgagatc cagtcgcgct tcccgttcgg ggtcatcatc ctcaagctgc acgacatgaa
      901 ggtccccccc atcgggccag tggcgggctg agcgtggcgt accgcgaagc cgacctctcg
      961 aaggtgcgca ccgtgccggt cggcacccgg cgcaacaagg tggaaggggc gctgctggcg
     1021 cacccccccg cggccgaccg ctcgttccat gccttcctcg acagcctccc agacatcctc
     1081 gccgcccgcg accttcggac ggtcatccag cacgtggcgg acgcagcccg ccgccggaag
     1141 ggcgtggtcc tccttctcgg cgggcacgtg atgaaggtcg ggatgggccc gctcatccgg
     1201 gcgtggctcg acgccgggat cgtctcccac atcgcgatga acggcgccgc tgccatccac
     1261 gacttcgagc tcgcggcatt cggcggcacc agcgaggatg tcgaagcggg gctgggggac
     1321 gggaccttcg ggatggcgga ggagaccggc gcggagatga acgcggccat cgcgctggcc
     1381 gcgtcccggg gccgcggcct cggcgaaggg ctggccgacg cgctcgccca gcgcgacacc
     1441 ctgccgggcc gcgaggtctc cgtcctcctc ggctgccggc agcacgacgt gccggtcacg
     1501 gtgcacgccg cgatcggcgc cgagatcatc caccagcacc cgaccaccga tggcgccgcg
     1561 ctcggagcca ccagccaccg cgatttccgg cgactggcgg ggtcgctgcc ggacctggat
     1621 ggcgggggcg cggtgctcaa ttgggggagc gcggtggtga tgcccgaggt gttcctcaag
     1681 gcgctcacca tcgcgcggaa catcggcgga gggcggcccc gggacttcct ggccgccgac
     1741 ttcgacatgc agcggcacta ccggccgcgg gtcaacgtgg tgcagcgccc caccaaggcc
     1801 ggtggcatcg gcatccagct caccggacac cacgaactcc tgctgccgat gctcatgtgg
     1861 ggcgtgctgg agaagcttgg agcctagagc ttagagctta gagcctagag cttagagcct
     1921 agagcttaga gcttagagcc tagagcctag agccaagagc ttagagctta gagcttggag
     1981 cctagagctt ggagatgatc cggcgtcagg cccggccctc ggctctgagc tccaagctct
     2041 cgactctgcc gctcagctct tccccttcat cgtatccgcg atcttgtccg cggtcttcgg
     2101 gctcagcacc cggatgacca gatagacgat gaagccgatc gccgcgagcc agagtagctt
     2161 gaacacgagg ccgacgacga tgccgaacag gccgagcacg atctggagcg cgaagtacgc
     2221 gaccaccgcg agcaccgcgt agccgagcag cgagcgaaac atcggagttc ctccggtgaa
     2281 gacaggtgca ccccagtacg gggaacgttt caaatagttt cgtcaggaat ataagtgcaa
     2341 gattcatttg acgttatcgt gatcggatcc ggggccatcg gtgccgcctg cgggcgggag
     2401 ctggcggtcg ccggccgccg ggtgctgctc ctcgaccgcg cgccccaagg gggggaggcg
     2461 tggcaggcag ccgccgggat gctggcgccg cagatcgaag ccggtccgga tgacccgatc
     2521 ttccagctgg cggtggccgc ccgggagcgc tacgtcgagc tggcccccgc gcttcaggaa
     2581 ggcgtcggga tcgacatcgg cctctggctc gaggggatcg cccgggtggc ggtcagcgcc
     2641 gaggacgtgc ccgagctccg ggcccgcgcc gcgtggcagc gccagcaggg ccacctctgc
     2701 gactggttcg acccgaccga ggtgaaggcg cgctacccct ggatgggggc caaccacggc
     2761 gcactctggg ccccacgcga tggcgcgctc gatcccacgg cgctggtccg ggcgctcagg
     2821 gccgacgccg tgcgcctcgg cgcccggcag gagatcgtgg agcgggtcga gatcctgcgc
     2881 gacggtgacc gggtgatcgg cgtccacggc gggcggaagt tctcctccga gcacgtggtg
     2941 gtggcggccg gggcgtggag tgccgacatc ggcggcctgc cccggccggt gtccgtggaa
     3001 ccggtgcggg gccagatggc ggcgctcccc tggcccgccg ggatcgaccc ggcgatcgtc
     3061 ttcggtggca ccgcttacgt ggtggcccgc ggcggcgagg cggtggtcgg gtccacgatg
     3121 gagtacgcag gcttcgacgc caccacgacg gcggccggtc tggcacgcat cttcgcaggc
     3181 gtgagcgcca tctgcccccc gctcgcaggc ctggaggtga agcggacctg ggcggggttg
     3241 cgaccgatga cccccgacgg actgccgatc atcggggcgg agccgcgcgc gcgggggctc
     3301 tggtacgcca ccggccacgg gcgcaacggg atcctcctgg ctgcgatgac gggcaccgtc
     3361 atcagcgacc tcatcgccgg caaggcagcc accgtggagg ggctgaccgc ggtgtccccg
     3421 gagcggttct ggcgctggtg acgggaccgt gatagcgtag aagttcctca caggggggcc
     3481 gatgcgtcct gggcgggctc tggcaggtct ggcctggttc ggcctcgcca ttggctgcaa
     3541 ctcacccggc gcgaccggcc cttccccgat cacgaccaat gacttccagc tcttgcccct
     3601 ccagcaatgg gccggcgagt cggtgcatgt ccgctccgcc gcattcgtct acctcaccga
     3661 cggtagctat cacatcctgg ccgggagcga cacgctcgcg gtgacccgga tcgacgattc
     3721 cacggtagcg gtcgcgccgc cggatgccgg gagccgcacg gtggcgttca ccatcacagg
     3781 ggccggcgcc accgcgcata tgggcagcgt gcagttcttc gggttcgggg gtatccgcga
     3841 acccgatgcg ggattcggcg gagactacga ggcgtggccc gccgggacca tcggcgcaac
     3901 gggcgtactc gggtgggtca acgacagcgg cacaggatac agcggcagca tcgcgacgct
     3961 cgacctccgc accggacacg tcaccactca cccgggcctc ggctatcacg gatcgtatgg
     4021 tccggctccc ggctatctac ccgatgtggt cacgacctcg ctcgacgtat ccccagtcgc
     4081 cgccgtccat ctgggcaatc cccacgacac gctggtcagc atcgcgatcc ccggattccc
     4141 gcgaaccgac tacctcatcg gcccgttcgt cgagttccac acgcaatcgc attcggccta
     4201 tgcccggaca agcaccgatt ccggcaggac ctggcatacc gtaaaggaca cgctctacct
     4261 gaattcgacc tggcagctgg tcctgtcccc gacgaagcga tcggccacgg tgatcaacgg
     4321 ctggtcggat tccggcggag tcgtcctcga cgcgacatcg ggactcaccc gctatcgatt
     4381 gacccggatg cgggaggtcg atgcggcggc cttctccgcc gacggcaccg tcctctatgt
     4441 agcgggcagc tcgactggtc ccgggatcgc tcagtccttg gaggccgtcg acgctgccac
     4501 cggtgccgcg ctccacgtgg attcgctcct cggcggcacc gccatctctg cagtggtcga
     4561 ccccgccgga ccccagctgt tcgtagcggt cgctcggcct gacgggacgc tcgcgctgaa
     4621 tgtctacgat gcgaccaccc tccggcggac cgcaacgctc cactcgactg ccaccgggtt
     4681 tgacctcacg cagttcatcc agtccggcgc gtcgatggcc gatcgggcag tctacgtcgc
     4741 gcgctcgggg ggaatgatcg cccgattcga catcctgccc cgcgccggcc aacccgctcc
     4801 cggacgccgc tgacatccgg cggcgcccct cccgccgtag gtggggcgtg gtgcactctt
     4861 tcccttcccg cccggggcgg gttatgatga tggcagccgc tctgacccgg gccgcccagc
     4921 ccgcgcgccg gacccggctc caggcgatca gcgctgtccc ccaacgacac ccaatgacca
     4981 agccggcccc gatcccccct tcgacggtcg acgaccaggc gctcatccag cgcgtggcct
     5041 ccggtgatga gcaggccatc ggccagctct acgatcggta cgcctccgtc ctccacgccg
     5101 cggcgtggcg gatcaccggc gagacggccg acgccgagga ggtgctcctc gacgcctttg
     5161 cccaggcgtg gcgtgacgcg tcgcgctacg atgcccagcg gggctcggtc ggcgcctggc
     5221 tgatgacgat cacccgctcc cgcgcgctcg atctgatccg ctcgcgcggc cgccgggagc
     5281 gcgccgcgac cgccgccggc gccgaccccg aggccgccgg gatcggcgcc agcacctggc
     5341 gctacgacac ctcccgcagc gccgaacaga gcgagcgccg ccgcctcatc agcatcgccc
     5401 tgcagagcct ctcggccgcc cagcgccagg ccatcgagct ggcgtactac gaggggctct
     5461 cgcattccga gatcgccgcg aagctcggcg agcccctggg caccgtgaag acgcgagtgc
     5521 gcctcggcct ccagaagctg cgcgaagcgt tgcggcccta ctacgggacg gcggcatgaa
     5581 cgagcgcgaa tccctccagg acctggccgc cgactacgca ctcggactcc tcggccccga
     5641 ggagaccgcg gcgttcgagc tcgagctcgc gcagtcgccg gggctccagc tcatcgtgcg
     5701 ggagtgccgc gaagcgctcg cggtgctccc gggcgggatc cagtcccccg cgccggccgc
     5761 ggacctccgc gcgcgggtga tcgcccgcgc gctcggcacc cccgccgacc cggcggcacg
     5821 ggacgcaacc gacgacatcg gatcggcggc cggaacggac accggtcccg ccgccgatga
     5881 cgacgtcgtc cttccgatca cgagcgaatc gccggtggcc cgggcgcccc gtcgccacgg
     5941 gagctcagcc ggctggtgga tggcactggc ggcaagcctc atcgccgcca ccgggctcgg
     6001 cgtcctctgg cggagcgcac ttcgggccgg gcaggcggca ctggtgcggg aggccgcgct
     6061 gcgtgcctcg gtcgactcgg tgtcgaagca gctggccgcc cgtgatgcca cgctccgggc
     6121 catcctcgcc ccgggcgtcg agctgcacct gctccggtcg accggggcgc cggaaccggg
     6181 gatcgagttc ttctggaaca agaagtccca ttcggcgctg atgtacgcct tcaacctgac
     6241 gccgtcggcc aagggccgga cctaccagct ctggatgatc cagggcgaca agcggatccc
     6301 atcggtcacc ttcgaggctg gcagcgatgg ccacgcgctg gtcgacaaga tcgccatgcc
     6361 gcccgacctc ccgttcgagg ccctcgcggt caccgatgag cccgccgggg gatcggcgca
     6421 accgacgacc ccggtgctgt tggtggcttc cataggatcg tagcggccgc gcggagcctc
     6481 gaaccgagca gagcccccgg gagtcccccg ggggctcgtc cgtcctgggg agtagtccgg
     6541 cgaccaggtg aagacgacgg agcatggaca ttcagcgtcc agggtcgagc gaaagatacg
     6601 tttcaactga atggttgaga aatccgactt tccccctccc cggcgggtag tccccccttt
     6661 accttcatgt ccgctgtaac ccgaccctct ggagcgtcga tggccagtcc ggcactgatc
     6721 cagatcaccc gccaccctgt tggcggcgcc ccgcaggggg cgccgttgat gcccgcgccg
     6781 ccggcgctcg agcgcaagcc ggggtggctc aaggtgaagg cgcccggcgg cccgagctac
     6841 ctcgacgtga agcggatgat gcgggagctc gacctccata cggtctgcga ggaggcccgc
     6901 tgccccaaca tcggcgagtg ctggg
//
LOCUS       DASUAP010000235         3936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1110124, whole genome shotgun sequence.
ACCESSION   DASUAP010000235 DASUAP010000000
VERSION     DASUAP010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110124"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFU45_06120"
     CDS             <1..615
                     /locus_tag="VFU45_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358672.1"
                     /translation="AALAPYRDSFTVLVRGTPLGYRTAELARDPAGWRYVDVTQIGAF
                     VLQRTEITLDRAGLVHQVLQGGQVQGIDIRTSVEYRRGRAVGVTVAPSGGKAVTLAVN
                     AAVPPGTVDDNAIPLYLPALTWDTDTAWALTVFVSGENALHTMRLHVTDTATVTVPAG
                     RFAVRVAELDGGPAPVRFCVTRAPPHRVVREELVGTPIEFVLVK"
     gene            791..2203
                     /locus_tag="VFU45_06125"
     CDS             791..2203
                     /locus_tag="VFU45_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5358673.1"
                     /translation="MHLTRRAGWLLAGALAGCAGHGGPGSPAPAPAPTGAAAAWSAER
                     PQYPSTYVRHSNPPVFIRNATVMTAAGPEITGASILFRDGKVVAVGKDLAAPADATVI
                     DGTGKFVTPGLIDDHSHLGVYAAPGTWAESDGNEATNPTTPNVWAEHSFWPQDPQIPL
                     AIAGGVTVIEALPGSANLIGGRSATLRLIPARTVQEMKFPGAPYALKMACGENPKRVY
                     QNRGPSTRMGNMAGYRAAFIQAEQYRAKWDKWQKDPVGDPPARDLGLETLADVLRGNI
                     QVQNHCYRADEMAQMIDLSKEFGFRIRSFHHVVEGYKVRDLLAKNDIAASVWADWWGF
                     KMEAYDGIPENAALLTSAGVRTVIHSDSEDGIQRLNQAAAEALYAGQQAGIPVTRLDA
                     IRWITANPAWVLRIEKETGTLEPGKDADVVLWSGDPFSVYSKADKVWNDGWLVFDRDD
                     PAHQYQTDFNVGQAAPGVGR"
     gene            2200..3483
                     /locus_tag="VFU45_06130"
     CDS             2200..3483
                     /locus_tag="VFU45_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5358674.1"
                     /translation="MSAYRQTAGGAAGGRALLCAIAMLAAAAPAAAQAVAITGGTVYP
                     VSGPRIENGTVILQDGKVVAVGAGIPVPAGATRIDATGKWVTPGFFHAFSDYGLTGVQ
                     SVDETREGSENGDVNPAFDVAEGIDPAAVTIPIARMQGITTALVAPSDRFLSGQGTVI
                     DLAGGTIEDMVGVDHAAMVIALGVQAKDAGGGSRAGVLERFRQLFRDALEYQRRKADY
                     DQNRMEQLSAPAAELAALAPVLSGKEPVIAQANRKSDIESALRLAREFHLRLVIRGGT
                     EAWMVAPELAQAGVPVLLQNLTDVPHFDGLNARLDNATILAQAGVAIVLAQNDDSRDR
                     DLRFAAGNAVRNGLSWDQALRAVTLAPAIALGVSDRWGSLEAGKTADVVVWSGDPFEF
                     SSRAEHVFIRGVDLPLKSRMTELLDRYRTLPPKYF"
     gene            complement(3513..3854)
                     /locus_tag="VFU45_06135"
     CDS             complement(3513..3854)
                     /locus_tag="VFU45_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358675.1"
                     /translation="MATFFERVNEAVGTAGRKAQAAMEQARLRIEQQRVRGERDHLLR
                     ELGQLFHREARGAAPDPARRDELFVAVDKADANLARIEKELAASKGEVVSVTDQPAAA
                     PPEPPTKPDGA"
BASE COUNT          623 a         1328 c         1438 g          547 t
ORIGIN      
        1 gccgcgctcg cgccgtaccg cgacagcttc accgtcctcg tccgcggcac gccgctgggc
       61 taccggaccg ccgaactcgc gcgcgatccc gccggctggc gctacgtcga cgtgacccag
      121 atcggcgcgt tcgtgctgca acggacggag atcacactcg accgggccgg gctggtgcac
      181 caggtcctgc aaggcgggca ggtccagggg atcgacatcc ggacgagcgt ggaataccgg
      241 cgggggcgcg ccgtgggcgt gacggtggcg ccgagcggcg ggaaggcggt gaccctcgcc
      301 gtcaacgcgg ccgtcccgcc cggcaccgtg gatgacaatg cgataccgct ctacctgccc
      361 gcgctcacat gggacaccga caccgcctgg gccctgacgg tgttcgtctc cggcgagaac
      421 gccctgcaca cgatgaggct gcacgtgacc gacacggcaa ccgtgacggt gccggcgggc
      481 cggttcgcag tgcgggtagc ggagctcgac ggcggcccgg cgccggtccg gttctgtgtg
      541 acccgggcac cgccccaccg cgtcgtgcgt gaggagctgg tggggacccc gatcgaattc
      601 gtcctggtca agtgaggaac gcgttgacga gcggtggaag gtgcgggtaa gcgcaggaag
      661 gcgggggaaa gcgggttaag gaagcgcgcc atagggagaa gcgagccgcg cgagtagctt
      721 tgcgggtgag cttggaaggt cgctccggca gcatccatga tcgttccaca tcaactgtcc
      781 gagaccaacg atgcatctga cccgtcgagc cggctggctg ctggcagggg cgctcgcagg
      841 ctgtgccggc cacggtggcc cgggaagccc ggcgcccgcg ccggcgccga ccggtgctgc
      901 ggccgcgtgg agcgctgaac ggccccagta cccgtcgacc tacgtccggc attccaatcc
      961 gccggtcttc atccggaacg cgacggtcat gaccgccgcc ggcccggaga tcaccggcgc
     1021 ctcgatcctc tttcgcgatg gcaaggtcgt ggcggtcggc aaggacctgg ccgcgcccgc
     1081 ggacgcgacg gtgatcgatg gcaccggcaa gttcgtgact cccggcctca tcgacgacca
     1141 cagtcacctt ggcgtctacg cggcgccggg gacctgggcc gagagcgacg ggaacgaggc
     1201 cacgaacccg accaccccga atgtgtgggc ggagcactcg ttctggccgc aggatccgca
     1261 gatcccgctg gcgatcgcgg ggggggtcac ggtcatcgag gcgcttcccg gatcggccaa
     1321 cctgatcggc ggccgctcgg cgacgctccg gctgatcccc gcacgcaccg tccaggagat
     1381 gaagttcccg ggggcgccgt atgccctcaa gatggcgtgc ggggagaacc cgaagcgggt
     1441 ctaccagaac cgcggcccct cgacgcggat ggggaacatg gcgggatacc gggcggcgtt
     1501 catccaggcc gagcagtacc gggccaagtg ggacaagtgg cagaaggatc cggtcggcga
     1561 tccgccggcg cgggacctcg ggctggagac gctggccgat gtcctgcggg gcaacatcca
     1621 ggtgcagaac cactgctacc gcgccgatga gatggcgcag atgatcgacc tctccaagga
     1681 gttcgggttc cggatccggt ccttccacca cgtggtggag gggtacaagg tccgggatct
     1741 cctggccaag aatgacatcg cggcatcggt gtgggccgac tggtggggct tcaagatgga
     1801 agcgtacgac ggcatcccgg agaacgccgc cctcctcacc agcgccggcg tgcggacggt
     1861 gatccactcc gacagcgagg acggcatcca gcgcctcaat caggccgccg ccgaggccct
     1921 ttacgccggc caacaggccg ggatccccgt gacgcggctc gacgcgatcc gctggatcac
     1981 ggccaacccg gcctgggtgc tccggatcga gaaggagacc ggcacgctcg agcccggcaa
     2041 ggacgccgac gtggtcctct ggtcgggcga cccgttctcc gtgtattcca aggcggacaa
     2101 ggtgtggaac gacggatggc tggtgttcga tcgcgacgac ccggcccacc agtaccagac
     2161 cgacttcaac gtgggccagg cggccccggg ggtgggacgg tgagcgcata caggcagacg
     2221 gccggcggag cggcgggcgg acgggcactg ctctgcgcca tcgccatgct ggcggcggcc
     2281 gcgccggccg cagcccaggc ggtcgcgatc accggcggca cggtctatcc ggtgtccggc
     2341 cccaggatcg agaacggcac cgtgatcctg caggacggca aggtcgtggc cgtgggcgcc
     2401 gggatccccg ttccggccgg cgccacgcgg atcgacgcga ccggcaagtg ggtgacgccg
     2461 gggttcttcc atgccttcag cgactacggg ctcaccggcg tgcagtcggt ggacgagacc
     2521 cgcgaaggtt cggagaacgg ggacgtgaac cccgccttcg acgtggcgga ggggatcgac
     2581 cccgcggcgg tcacgatccc gatcgcccgg atgcagggga tcaccaccgc cctggtggcg
     2641 ccaagcgacc gattcctgtc gggtcagggg acggtgatcg acctggcggg cgggacgatc
     2701 gaggacatgg tgggcgtcga ccacgcggcg atggtgatcg cgctcggggt ccaggccaag
     2761 gacgcagggg gcggcagccg cgcgggggtg cttgagcggt tccgccagct cttccgggac
     2821 gcactcgagt atcagcgccg caaggccgac tacgaccaga accggatgga gcagctctcc
     2881 gcgccggcgg cggagctggc ggccctggcg ccggtcctga gcggcaagga gccggtgatc
     2941 gcccaggcca accggaagag cgacatcgag agcgcgctgc gcctggcaag ggaattccac
     3001 ctccgcctgg tcattcgcgg cggcactgag gcgtggatgg tggccccgga gctggcgcag
     3061 gccggcgtgc cggtgctgct ccagaacctg accgacgtcc cacacttcga cggcctcaac
     3121 gcgaggctcg acaatgccac gatcctggcg caggccgggg tggccatcgt cctggcgcag
     3181 aacgacgaca gccgcgaccg ggacctccgc ttcgcggccg gcaacgccgt ccgcaacggg
     3241 ctatcctggg atcaggcgct tcgcgccgtg acgctggcgc ccgccatcgc gctcggtgtg
     3301 agcgaccggt ggggatcgct cgaggccggg aagacggccg acgtggtggt ctggtcgggg
     3361 gatccgttcg aattctcgag ccgggcggaa catgtgttca tccggggcgt cgacctgccg
     3421 ctcaagagcc ggatgaccga gctgctggac cgataccgga cgctcccgcc gaagtatttt
     3481 tagaggcggg agcggatcgc gcgtcgcgcc gcctacgcgc cgtcgggctt ggtgggcggc
     3541 tccggcgggg cggcggccgg ctggtcggtg acgctcacga cttcaccctt gctggccgca
     3601 agctccttct cgatccgggc caggttggcg tcggccttgt ccaccgcgac gaagagttca
     3661 tcgcgccggg cgggatcggg cgccgcgcca cgcgcctcac ggtggaagag ctgcccgagt
     3721 tcccgcagca ggtggtcccg ctcgccgcgg acccgctgct gctcgatccg gagccgggcc
     3781 tgctccatcg ccgcctgggc cttccggccg gcggtcccca ccgcttcgtt gacccgctcg
     3841 aagaaggtcg ccatcggtca cctcgctgag agtcccgtcc ccgtacgcat ccgcaccatc
     3901 gatgtttcag attgcacagg tggcaggcaa aagaaa
//
LOCUS       DASUAP010000236         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_838527, whole genome shotgun sequence.
ACCESSION   DASUAP010000236 DASUAP010000000
VERSION     DASUAP010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838527"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFU45_06140"
     CDS             <1..906
                     /locus_tag="VFU45_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEU5358676.1"
                     /translation="PVRLLIAFLIVGLFGLALPHALHAWTPGTHIYLAESVLQRLELL
                     PGLAGDLLRAFPYDYLYGSIAADTSFAKKYAPSGRHPHAWHVAQEIHDTAPSDALKAF
                     GLGYLSHLAADVIAHNHFVPRQLVLSRGPSGVAHPYWEARVEHLLGEHYSRSAVDLIR
                     MDHLRADRHLDTILSPTLFSVKTNRRMFRGMVRLGEQRTLQATLRMADEYGKWALTEE
                     DVGRHMDRSLAQIVQLLKGEEPEVRLADPSGYAALAKAREIRKIAFQHGYRGNPKRVI
                     EDAEEEFGLSRPPLHVPPEPEPPTK"
     gene            complement(1063..2481)
                     /gene="gatB"
                     /locus_tag="VFU45_06145"
     CDS             complement(1063..2481)
                     /gene="gatB"
                     /locus_tag="VFU45_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU5358677.1"
                     /translation="MTWETIIGLEVHVQLKTRSKMFCGCSANFGDAPNTNVCPVCLAL
                     PGALPVPNAEAIRLATLAALALNCTVHERSVFARKNYFYPDLPKGYQISQFDRPLATK
                     GWVEIDTAERGRLRIGVTRLHVEEDSGKSVHDRIPGRTAIDLNRAGVPLAEIVSEPDL
                     RSPAEARAYLTALRQILVFAGVSDCSMEEGSLRVDGNISIHRPGTPFGTKTEVKNLNS
                     FANVERALELEQGRQVEVLESGGRVEQVTMLYNAGADAMRVMRSKEDSRDYRYFPDPD
                     LPPLLLSRSWIDARGEELPELPDQKRQRLIAKYGIPAYDAGVLTGEPVLADHFEAVVA
                     AGADAKLASNWVMGDVISGWNETGKLEVSAPRLAALIALVTSNTVSHASARKIYAEMR
                     TSFDEPAALADKLGLRQVADTGVLERWVDDVLAANPAEVARYRGGEVKLIGFFVGQVM
                     KKSGGKADPKAVQPVLVEKLKG"
     gene            complement(2481..>3238)
                     /locus_tag="VFU45_06150"
     CDS             complement(2481..>3238)
                     /locus_tag="VFU45_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU5358678.1"
                     /translation="DPLDATTADRPPLTLDEPRRDLTGLTIGLPREYFPADLHAGVRA
                     ALDQTIAKVRELGATVREVSLPYTRFAVPAYYIVAPAEAAANLARFDGVRYGPRGGAA
                     SADLNQMYRDTRGRGFGPEVRRRILVGTYVLSAGYHDQYYHRAQQVRGLIARDFDTVF
                     ASGVDLLLTPTTPTPAFKAGEKMGDPVSMYLADIFVCPSSLSGHPALSLPVGRDQGLP
                     IGAQLIAPMWQEARMLSAASAIERVTDAKREVR"
BASE COUNT          466 a         1152 c         1127 g          493 t
ORIGIN      
        1 ccggtccgcc tcctgatcgc cttcctgatc gtggggctct tcgggctggc cctcccccac
       61 gcgctccacg cctggacccc cggcacccac atctatctgg cggagtcggt gctgcagcgg
      121 ctggagctgc tgccggggct cgccggcgac ctcctccgcg ccttccccta cgactacctc
      181 tacggctcga tcgcggcgga cacgagcttt gccaagaagt acgcgccgag cggccggcac
      241 ccccacgcct ggcacgtggc gcaggagatc cacgacaccg cgccgtccga cgccctcaag
      301 gcgttcggcc tgggctacct ctcccacctg gccgccgacg tcatcgccca caaccacttc
      361 gtcccccgcc agttggtgct ctcccgcggc ccctcggggg tcgcccaccc ctactgggag
      421 gcgcgcgtcg agcacctcct gggagagcac tactcccgct cggcggtgga cctgatccgg
      481 atggaccacc tccgcgccga ccggcacctc gacacgatcc tctcccccac cctcttcagc
      541 gtgaagacca atcgccggat gttccgcggg atggtgcgcc tgggggagca gcgcaccctc
      601 caggcgacgc tccggatggc ggatgaatac gggaaatggg cgctgaccga ggaggacgtg
      661 ggccggcaca tggaccgctc gctcgctcag atcgtccagc tcctgaaggg cgaggagccc
      721 gaggtgcgcc tggccgaccc gtcggggtac gccgcgctcg ccaaggcgcg cgagatccgg
      781 aagatcgcct tccagcacgg ctaccggggg aaccccaagc gggtgatcga ggacgccgaa
      841 gaagagttcg gcctttcccg gcccccgctc cacgtccctc cggagccgga gccccccacc
      901 aaatgacgtg ccccctctcc gcgcatcagc gtggagaggg ggacaggggg tgaggctttg
      961 gtggcgggcc gatccttcgg tcgcttcgct ccctcaggat gacggggcga cgggtcgccg
     1021 caggcgaccc ccacaacaac gcaggcgacc cccacgccac cattacccct tcagcttctc
     1081 cacgagcacc ggctgcaccg ccttcggatc cgccttcccc ccgctcttct tcatcacctg
     1141 ccccacgaag aacccgatca gcttcacttc cccgccccgg taccgcgcca cctcggcggg
     1201 gttggccgcg agcacgtcat ccacccagcg ctccagcact cccgtatccg ccacctggcg
     1261 gagcccgagc ttgtcggcca gggccgccgg ttcgtcgaat gaggtgcgca tctcggcgta
     1321 gatcttccgg gccgaggcgt ggctcaccgt gttgctggtg acgagcgcga tgagcgccgc
     1381 gaggcgcggg gcggagacct cgagcttccc ggtctcgttc cagccggaga tgacgtcgcc
     1441 catcacccaa ttggaggcga gcttggcatc cgcccccgcc gccaccacgg cctcgaagtg
     1501 atcggccagg accggctcgc cggtgaggac cccggcgtcg tacgccggga tcccgtactt
     1561 cgcgatgagc cgctgccgct tctggtcggg gagctcggga agctcctccc cccgcgcgtc
     1621 gatccatgac cgcgagagca ggagcggcgg aaggtcgggg tcggggaagt agcggtagtc
     1681 gcggctgtct tccttggagc gcatgacccg catcgcatcg gcgccggcat tgtagagcat
     1741 cgtcacctgc tccaccctgc cccccgactc gagcacctcc acctgccgcc cctgctccag
     1801 ctcgagcgca cgctcgacgt tggcgaagga gttgaggttc ttgacctcgg tcttggtgcc
     1861 gaagggtgtg cccggccggt ggatcgagat gttgccgtcc acccggagcg acccttcctc
     1921 catcgagcag tcgctcacgc cggcaaagac gaggatctgg cggagtgcag tgagatacgc
     1981 gcgtgcctcc gccgggctcc ggaggtcggg ctcgctcacg atctcggcga ggggcacgcc
     2041 cgcgcggttc aggtcgatgg cggtgcggcc ggggatccgg tcgtggacgc tcttgcccga
     2101 gtcctcttcc acgtggagcc gggtgacgcc gatgcggagc cggccccgtt cggcggtgtc
     2161 gatctcgacc caccccttcg tggcgagcgg ccggtcgaac tgggagatct ggtatccttt
     2221 ggggaggtcg ggatagaagt agttcttccg cgcgaagacg ctccgctcat gcacggtgca
     2281 gttgagcgcc agcgcggcca gggtggccag ccggatggcc tcggcgttgg gcaccgggag
     2341 ggcgccgggg agcgccaggc acacggggca gacgttcgtg ttcggcgcgt cgccgaagtt
     2401 ggcggagcag ccgcagaaca tcttggaccg cgtcttcagc tggacgtgga cctcgaggcc
     2461 gatgatcgtc tcccaggtca tcagcgcacc tcccgcttcg cgtcggtgac gcgttcgatg
     2521 gccgaggccg ccgagagcat ccgcgcctcc tgccacatcg gggcgatgag ctgggcgccg
     2581 atcgggagcc cctggtcgcg tccgaccgga aggctcaatg ccgggtggcc ggagagcgag
     2641 gaggggcaga cgaagatgtc ggccaggtac atggacaccg ggtcgcccat cttctccccc
     2701 gccttgaagg cgggggtggg cgtcgtgggg gtgaggagga ggtcgacgcc ggaggcaaag
     2761 acggtgtcga agtcccgggc gatgagcccc cggacctgct gggcgcggtg gtagtactgg
     2821 tcgtggtagc cggcgctcag cacgtaggtg cccaccagga tccggcgccg cacctcggga
     2881 ccgaagccgc ggccgcgggt gtcgcggtac atctggttca ggtcggcgct cgcggcaccg
     2941 ccgcggggac cgtaccgcac gccgtcgaag cgggccaggt tggccgccgc ctcggccggc
     3001 gccacgatgt aatacgcggg caccgcgaac cgggtgtacg ggagcgacac ctcccgcacc
     3061 gtggcgccga gttcccggac cttggcgatg gtctggtcga gcgcggcgcg gacgccggcg
     3121 tggaggtcgg ccgggaaata ctcccgcgga aggccgatcg tgaggcccgt caggtcgcgg
     3181 cgcggctcgt cgagggtgag cggcgggcgg tcggcggtgg tggcatcgag cgggtcgt
//
LOCUS       DASUAP010000237         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_306400, whole genome shotgun sequence.
ACCESSION   DASUAP010000237 DASUAP010000000
VERSION     DASUAP010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306400"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFU45_06155"
     CDS             <1..745
                     /locus_tag="VFU45_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358679.1"
                     /translation="NIGTGGTLVMTNTADSLEVVQGATFGGNSTAGLLTAGTLILDGD
                     FTQTGDPKAFAPSGTHLTVFNPATRSGGVTHAISFSNPTTSTFQDLREDYPDTLSIFN
                     GEADVHGTLTVNHAALQNGLVIRSGLGNGLLRFGTADVHAAVFDTVTAQFLRTTTPLV
                     AFDSVTFNAPTDGFTQFMANGHAGDTLTISNTVFTTVGTGTGNYISATNLDGTGLFTV
                     LALLPNLGSAEFNAHILPDPFSTIVWDVP"
     gene            complement(950..>2929)
                     /locus_tag="VFU45_06160"
     CDS             complement(950..>2929)
                     /locus_tag="VFU45_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5358680.1"
                     /translation="IRAELDARASALRQSTAIFAKQSPVIARIEKAVSDSQTSDLLDA
                     DSELVQQTGAAWALVTDGSGILRAWSLHRDEIGDDFSGASLVAKALQGQAASGVWIEV
                     DSSSGTPHDVIYQAVAVPVRRAGSNPIAAFVAALPVDSAFARSLNQQTASDIVFFTLD
                     TLGHPRVTLSTLPTASGMAEAMAGMSMGQMGMAVDSTLQAAGDSGRVTLAGGGDVWVG
                     TVGGLLNADGQPLGGYVGLRSRARELAAYTGLERTMVVVFVIGVLLALIISSLVARQV
                     TRPIERLVQATREVREGRFSGAIDIRSRDEVGELAGAFRRMMADLKEKQDLVEYLSAA
                     GVGSTVQMTPAERQGVNSGEILPPMRPSQVSSLLAPGSTFAGRYTIREILGAGGMGVV
                     YRAMDTQLHEPVAIKTLKPDAMGAENVERFKQEIRLARRITHRNVVRTHDLGEADGTL
                     FITMEYVEGKSLAEVLKKRGRLPVGVALTVARQLCRALEAAHEMGVIHRDIKPQNLVV
                     DPTGVLKVMDFGIARLSEGRPEGAKGLTAVGTVIGTPEYMSPEQLMGAELDARSDLYS
                     AGAVLFECVTGRAVFEAPNVTALIIKHVEEEPVDPRTLVPEVPHDLALLILKALAKAP
                     AARFQSAAEMGEALDEVEVAPERVSGAVKALSGQA"
BASE COUNT          429 a         1120 c          916 g          464 t
ORIGIN      
        1 caacatcggc accggcggca cgctcgtgat gaccaacacc gccgactccc tcgaggtggt
       61 ccagggcgcc accttcgggg gcaactcgac cgccggcctc ctcacggccg ggacgctgat
      121 cctcgacggc gacttcacgc agactggcga cccgaaggcc tttgccccct cgggcacgca
      181 cctgaccgtg ttcaaccccg ccacccgctc gggcggcgtc acccatgcca tctcgttctc
      241 gaacccgacg acgtccacgt tccaggacct gcgcgaggat taccccgaca cgctgtcgat
      301 cttcaacggg gaagcggacg tccatggcac gctgaccgtg aaccacgcgg cgctccagaa
      361 cgggctcgtc atccgctccg ggctcggcaa cgggctgctc cggttcggca ccgctgacgt
      421 ccacgcggcg gtcttcgaca ccgttaccgc ccagttcctg cgcaccacga cgccgctcgt
      481 ggccttcgac agcgtgacgt tcaacgcccc gaccgacggg ttcacgcagt tcatggcgaa
      541 cgggcacgcc ggcgacacgc tcaccatcag caacacggtg ttcacgaccg tcggcacggg
      601 gactggcaac tacatctctg ccaccaacct cgacggcaca ggtctcttca ccgtgctggc
      661 gctcctcccg aacctgggca gcgccgagtt caatgcccac atcctgccgg atccgttcag
      721 tacgatcgtg tgggatgtgc cgtgaggggt gaacggtgaa cggtcgaccg tgaacgggac
      781 gtgagcgggg ggagcggcgt ggactcacac gcgctccccc cgttcacatc gtttgccaca
      841 tgcccgtcac ggtacctcgc acccactggc cctcgcctct tccgcctggc atcctccctc
      901 ttacctattc cctattccct cttccctctc acgtctcacg tcttacgtct cacgcctgac
      961 cgctcagcgc cttcaccgcc ccgctcaccc gctccggcgc cacctccacc tcgtcgagcg
     1021 cctcccccat ctccgcggcg ctctggaacc gggcggcggg cgccttggcc agcgccttga
     1081 ggatgaggag cgccaggtcg tgcggcacct ccggcaccag cgtccgcgga tcgaccggct
     1141 cctcctcgac gtgcttgatg atgagcgcgg tgacattcgg cgcctcgaac accgcccgcc
     1201 cggtgacgca ctcgaagagc accgcccctg ccgaatagag gtcggagcgc gcgtcgagct
     1261 cagcgcccat cagctgctcc ggcgacatgt actccggcgt cccgatcacg gtgccgacgg
     1321 cggtgagccc cttggccccc tccggccgcc cctcgctgag ccgcgcgatg ccgaagtcca
     1381 tcaccttgag gacgccggtc gggtcgacca ccaggttctg cggcttgatg tcgcggtgga
     1441 tgacgcccat ctcgtgcgcg gcctcgagcg cccggcagag ctggcgcgcc acggtgagcg
     1501 ccacccccac cgggagccgc ccccgcttct tgagcacctc cgccaggctc ttcccctcca
     1561 catactccat ggtgatgaag agcgtgccgt cggcctcgcc gaggtcgtgg gtgcgcacca
     1621 cgttgcggtg ggtgatccgc cgcgccaggc ggatctcctg cttgaaccgc tcgacgttct
     1681 ccgcccccat cgcgtcgggc ttgagggtct tgatggccac cggctcgtgg agctgggtgt
     1741 ccatcgcacg gtacaccacc cccatcccac cggcgccgag gatctcccgg atggtgtagc
     1801 gccccgcgaa ggtcgacccc ggcgccagca gcgacgacac ctggctcggc cgcatcggcg
     1861 ggaggatctc gccgctattg acgccctggc gctcggccgg cgtcatctgc accgtgctcc
     1921 cgacgccggc ggcgctcagg tattcgacca ggtcctgctt ttcctttagg tcggccatca
     1981 tccggcgaaa tgcgccggcc aactcaccga cctcgtccct cgagcggatg tcgatcgccc
     2041 cgctgaagcg cccctcccgc acctcgcgcg tggcctggac cagccgctcg atgggccggg
     2101 tcacctgccg cgccaccagg ctcgagatga tgagtgccag caggacgccg atcacgaaca
     2161 ccacgaccat cgtccgttcg aggccggtgt acgccgccag ctcccgcgcc cgcgaccgga
     2221 gtcccacata gccgccgagc ggctgaccgt cggcgttgag gagccccccc acggtgccca
     2281 cccagacgtc gccgccgccg gccagcgtca cccgccccga gtcgcccgcg gcctggaggg
     2341 tactgtccac tgccatcccc atctgtccca tcgacatccc ggccatcgcc tcggccatcc
     2401 cggacgcggt gggcagggtg gagagggtga cgcgcgggtg gccgagggtg tcgagggtga
     2461 agaagacgat gtcggaggcg gtctgctggt tgagcgaacg ggcaaaggcg gagtcgaccg
     2521 gaagcgccgc cacgaaggcc gcgatgggat tgctccccgc ccgccgcacc ggcaccgcga
     2581 cggcctggta gatcacgtcg tgcggcgtgc cgctgctcga gtcgacctcg atccacactc
     2641 ccgaggccgc ctgcccctgc aacgcctttg ccacgagcga cgcgccgctg aagtcgtcgc
     2701 cgatctcgtc gcggtggagc gaccaggcgc ggaggatgcc ggatccgtcg gtgacgagcg
     2761 cccaggcggc gccggtctgc tggaccagct cggaatcggc gtcgaggagg tcgctcgtct
     2821 ggctgtcgct caccgccttc tcgatccggg cgatgacggg gctttgcttg gcgaagatcg
     2881 ctgttgattg ccggagggcc gaggcccgcg cgtcgagctc ggcgcggat
//
LOCUS       DASUAP010000238         4657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_333791, whole genome shotgun sequence.
ACCESSION   DASUAP010000238 DASUAP010000000
VERSION     DASUAP010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333791"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1675
                     /locus_tag="VFU45_06165"
     CDS             <1..1675
                     /locus_tag="VFU45_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358681.1"
                     /translation="GLLAIEAGLALVGPELTQRAIDVAIPAGDGGLLLTLAGVYVASL
                     VLSFGADYGGAFITTLIGQRVMNDMRRQIFGHLQRLSIPYFDRNPVGRLVTRVTSDVE
                     TLNDLLSSGFVTVIGDVMTLAAIMILMLVMDWRLALVAFAVMPVVFLVAVVFRRHVRQ
                     AFRDIRTRLARLNAFLTEHLTGMRVVQLFGRERETWERFDRVNADHLEAHLRSITIYA
                     VFLPVMEVLTSVSLALLIWYGGLRSLGGSLTVGVLAAFIQLTRRFFQPLQDLSEKFNL
                     LQSAMASSERIFTLLDQPVTVPAPAAPVRLARPVRGEVRFENVSFRYSPEGPWVLKNV
                     SFTASPGRTLALVGYTGAGKTTIISLLLRFYDVTEGRVTVDGVDVRELDPGELRRLIG
                     FVQQDLFLFAGDVLRNLVLDTGVTEAQAREAARRVGAERFIERLPAGYGHVLGERGRS
                     LSVGERQLLSFARALALDPAILILDEATSSVDAESEALIQRALLELMSGRTSIVVAHR
                     LSTILHADEILVLHHGEVRERGSHRELLAAGGLYERLYRLQIASAAGAA"
     gene            1777..3210
                     /locus_tag="VFU45_06170"
     CDS             1777..3210
                     /locus_tag="VFU45_06170"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5358682.1"
                     /translation="MLQLEFGGQKYPIPVPEAVVGSEPGLAITLAGAGVQPRHAVLQS
                     AADGSVSVRALAAGVELSLNGVKVGTDPHPILHGDKIKVGSHELLVVDPQRSGSTQYV
                     SASDLANAVASVKKGGAAKPTAGLVNGRLVCLTDGREYAINRASLIMGRDASCDVVVE
                     SKNVSRKHAEIVTSPNGYLLIDHSTNGTLVNGEKIQGQRLLARSDMIRVGEDDFRFYA
                     DQPPGGGQPAPPPAAAPVAAPPPAAPAAQQPSAPPPGARQRLHDTMHGAPPAAAAAPA
                     PGPLASLLIRSGARKGERVAIRVPVVNIGRADYNDVVLAEESVSTQHAKLQRREGVWM
                     LVDLGSTNGSFVDGERIEGEAPVTPGATLRFGDASVIFDPKDEGVEVAKGGSTKMISA
                     IPVAPPKPAAPAARTTAPVSQPTPAPAAAPAAPKAPGAKPSAPKPAPRPAAPVKAPAA
                     GGGLPKWVIPAAVVLVIVIVAIIFLLK"
     gene            3215..3943
                     /locus_tag="VFU45_06175"
     CDS             3215..3943
                     /locus_tag="VFU45_06175"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU5358683.1"
                     /translation="MRFSCAARTDVGIVRSGNEDNYLMLDERGVFIVADGMGGHAAGE
                     VASEMAVHTIAKELGTVRGMTDEEIGERMRSAIRAANAAIFERTLTEHDKRGMGTTAT
                     ALVLLAGRYLLGQVGDSRGYLLRDGELFQVTKDHSYVQEQVDAGLLTPEQARVHPYSN
                     VITRCVGANADVLPDLYFGALRPGDVLLLASDGLTGMLEDEHLQKILTSPGGPQEWVD
                     AMITDANRRGGLDNITAIIVRIEE"
     gene            complement(4226..>4657)
                     /locus_tag="VFU45_06180"
     CDS             complement(4226..>4657)
                     /locus_tag="VFU45_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358684.1"
                     /translation="VHERRLHKRAVDLAAADLPADTQAWISEDPRLVERVEDALAAEL
                     GLPAGGLLLDFPSRSTMLSVDLPLRTRTGETARLTDAGRAGQLGLPRVAEELYQSARR
                     FRVFTAGRVAIRLESVTEVLTWPAAEVAKRLDVGAPFFGKS"
BASE COUNT          675 a         1700 c         1593 g          689 t
ORIGIN      
        1 cgggctcctg gccatcgagg cgggcctggc gctggtggga ccggagctca cccagcgtgc
       61 catcgacgtg gccatccccg ccggggacgg cgggctcctg ctcacgctgg cgggcgtgta
      121 cgtggcgtcg ctcgtcctga gcttcggcgc cgactacggc ggcgcgttca tcacgacgct
      181 gatcggccag cgggtgatga acgacatgcg gcgccagatc ttcgggcacc tgcagcggct
      241 cagcatcccc tacttcgacc gcaaccccgt cggacggctt gtcacccgcg tgaccagcga
      301 cgtcgagacg ctgaacgacc tgctctcgtc gggattcgtg accgtgatcg gtgacgtgat
      361 gaccctggcg gccatcatga tcctgatgct ggtgatggac tggcggctgg cgctggtggc
      421 gttcgcggtg atgccggtgg tgttcctcgt ggcagtcgtc ttccggcgcc acgtgcgcca
      481 ggccttccgc gacatccgca cccggctcgc ccggctcaat gcgttcctca ccgagcacct
      541 gaccgggatg cgcgtggtgc agctcttcgg ccgcgagcgg gagacctggg agcgcttcga
      601 ccgggtcaac gccgaccacc tcgaggcgca cctgcgctcg atcaccatct acgccgtctt
      661 cctgccggtg atggaagtgc tcacctcggt gtcgctggcg ctcctcatct ggtacggcgg
      721 gctccgatcg ctcggcggga gcctcacggt gggcgtgctg gcggccttca tccagctcac
      781 ccggcgcttt ttccagccgc tgcaggacct gtcggagaag ttcaacctgc tgcagagcgc
      841 gatggcgtcc tcggagcgga tcttcaccct gctcgaccag ccggtgaccg tgccggcgcc
      901 ggcggccccg gtgcggctgg cccggcccgt ccgcggcgag gtgcgcttcg agaacgtctc
      961 cttccgctat tcccccgagg gaccctgggt gctcaagaac gtgagcttca ccgcgagccc
     1021 cggacgcacg ctcgcgctcg tcgggtacac cggggcgggg aagaccacga tcatcagcct
     1081 cctcctccgg ttctacgacg tgaccgaggg ccgggtgacc gtcgacgggg tcgacgtgcg
     1141 cgaactcgat ccgggagagc tccggcggct catcgggttc gtgcagcagg atctcttcct
     1201 cttcgcgggc gacgtgctca ggaacctggt cctcgacacc ggcgtcaccg aggcgcaggc
     1261 gcgggaggcg gcgcggcgcg tgggggccga gcggttcatc gagcggctcc ccgcggggta
     1321 cggccacgtg ctgggggagc gggggcggag cctgagcgtg ggggaacggc agctgctgag
     1381 ctttgcgcgg gcgctggcgc tcgatccggc gatcctcatc ctcgacgagg ccaccagttc
     1441 ggtcgacgcc gaatcggagg cgctcatcca gcgggcgctc ctcgagctga tgtcggggcg
     1501 gacctcgatc gtggtggcgc accggctcag caccatcctc cacgccgatg agatcctggt
     1561 gctgcaccac ggggaggtcc gggaacgcgg gagccaccgg gaactcctcg ccgccggggg
     1621 cctctatgag cggctctacc ggctgcagat cgcctcggcg gccggggcgg cctgagcggg
     1681 gccgcgggcg tcgaaggcgt aacttggcct cgcttgcgca cgtagaaggg ggcgcggtag
     1741 ctttccccct tcattgattc cgcccgggac aggtacatgc tgcagctaga gttcggtggt
     1801 cagaagtacc cgatccccgt ccctgaggct gtggtcggga gcgaacccgg cctggccatc
     1861 accctcgccg gcgcgggggt ccagccgcgc cacgcggtgc tccagagcgc ggccgacggc
     1921 tcggtgtcgg tgcgggcgct ggcggccggg gtcgagcttt cgctcaacgg ggtcaaggtc
     1981 ggcaccgacc cgcatccgat cctccacggc gacaagatca aggtcggctc ccacgagctg
     2041 ctggtggtgg acccccagcg gagcgggagc acgcagtacg tgagcgcgtc cgatctcgcc
     2101 aacgccgtcg cctcggtcaa gaagggcggg gcggccaagc cgaccgcggg gctcgtcaac
     2161 ggacggctgg tgtgcctgac cgacggccgg gagtacgcga tcaaccgtgc gtcgctcatc
     2221 atggggcgcg acgcgagctg cgacgtggtg gtggagagca agaacgtctc ccggaagcac
     2281 gcggagatcg tgacctcgcc caatggctac ctgctgatcg accacagcac caacggcacg
     2341 ctggtcaacg gcgagaagat ccagggccag cggctcctgg cccgatccga catgatccgc
     2401 gtgggcgagg acgacttccg cttctacgcc gaccagccgc cgggtggcgg ccagccggcg
     2461 cccccgcccg ccgcggcacc ggtcgccgct cccccgccgg ccgctcccgc cgctcagcag
     2521 ccctccgcgc cccctccggg ggcgcgccag cgattgcacg acacgatgca cggcgcgccc
     2581 ccggcggccg ccgcggcccc cgccccgggg ccgctcgcgt ctttgctcat ccggagcggc
     2641 gcccgcaagg gcgagcgggt ggccatccgg gtgccggtgg tcaacatcgg ccgcgccgac
     2701 tacaacgacg tggtgctggc cgaggagagc gtgagcaccc agcacgccaa gctgcagcgc
     2761 cgcgagggcg tctggatgct ggtggatctc ggctccacca acggctcgtt cgtcgacggc
     2821 gagcggatcg aaggcgaggc cccggtcacg cccggcgcca cgctccgctt cggcgatgcc
     2881 tcggtcatct tcgaccccaa ggacgagggc gtcgaagtgg ccaagggcgg gagcaccaag
     2941 atgatcagcg ccatcccggt ggcccccccc aagccggcgg cccctgcggc ccggaccacg
     3001 gcaccggtgt cgcagcccac gccggccccc gccgccgcgc cggcagcacc caaggccccc
     3061 ggcgccaagc cgtccgcccc caagcccgcg ccccggcccg ccgcaccggt caaggctccg
     3121 gccgccggtg gtgggctccc caagtgggtc atccccgccg cggtcgtgct ggtgatcgtg
     3181 atcgtcgcca tcatcttcct cctcaagtaa tcccgtgcga ttctcctgcg cggcccgcac
     3241 cgacgtcggc atcgtccgct ccggcaacga ggacaactac ctcatgctgg acgagcgcgg
     3301 cgtcttcatc gtcgccgacg ggatgggtgg acacgccgcc ggcgaggtgg cgagcgagat
     3361 ggccgtccac accatcgcca aggaactcgg caccgtccgg ggcatgaccg acgaagagat
     3421 cggcgagcgg atgcgctcgg ccatccgcgc cgccaacgcg gcgatcttcg agcggaccct
     3481 cactgagcat gacaagcgag ggatgggcac caccgccacc gccctggtcc tgctggcggg
     3541 gcgctacctg ctgggccagg tgggcgacag ccggggctac ctgctgcggg acggcgagct
     3601 cttccaggtg accaaggacc actcctacgt gcaggaacag gtcgatgccg ggctcctgac
     3661 gccggagcag gcacgggtcc atccctacag caacgtcatc acccgctgcg tcggcgccaa
     3721 cgcggacgtc ctccccgacc tctatttcgg cgccctccgc cccggcgacg tgctgctcct
     3781 cgcctccgac gggctcaccg ggatgctcga ggacgaacac ctgcagaaga tcctcacctc
     3841 gcccggcggt ccgcaggaat gggtcgacgc gatgatcacc gacgccaacc ggcggggcgg
     3901 gctcgacaac atcacggcga tcatcgtgcg gatcgaggag tgagggcggc gagggaagag
     3961 tgaagaggga aaagtgaaaa gggaaaagtg aaaagtgaca agtgaaaagg gggctccggt
     4021 gtcgatcgga gccccctttc gcgcgttctc gccttcgtgc ccgccttcga ccttccgccg
     4081 ccttccttcg ctcagctcct gtcgaggaag ggtgctccct ttcgcgcgtt ctcgccctcg
     4141 cgccaacctt caaccttcca ccgcgttcct tcgccttccc ccgccttcct tcgccttccc
     4201 cccgccttcc ttcgcattcc ttcgctcaac tcttcccgaa gaacggtgct cccacatcga
     4261 gccgcttcgc gacctccgcc gccggccagg tcagcacctc cgtcaccgat tccaggcgga
     4321 tcgccacccg cccggccgtg aacacccgga accggcgcgc gctctggtac agctcctccg
     4381 ccacccgcgg gagccccaac tgccccgccc ggccggcgtc ggtcaggcgc gcggtctcgc
     4441 cggtgcgggt gcggagcgga aggtccaccg agagcatcgt ggaccggctc gggaaatcga
     4501 gcagcagccc acccgccggg aggcccaact cggccgccag cgcatcttcg acccgctcca
     4561 ccaggcgggg gtcctccgag atccaggcct gggtgtcggc ggggaggtca gcggcggcaa
     4621 ggtccacggc ccgcttgtgg aggcgccgct cgtggac
//
LOCUS       DASUAP010000239         6141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_333851, whole genome shotgun sequence.
ACCESSION   DASUAP010000239 DASUAP010000000
VERSION     DASUAP010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333851"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VFU45_06185"
     CDS             complement(<1..1314)
                     /locus_tag="VFU45_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5358685.1"
                     /translation="MTGPGQLRRVLGVGDVVLFFIVAVVGLRWIAAAAAAGPSSLVIW
                     AIACGAIFIPSAFTVLELSSRYPEEGGLYLWVKEAFGERAGFIAGWLYWASNLVYFPG
                     LLYFAAGNALFIGGSHWIGLSGSATYYIVFSLFGLALACWLNVVGLDVGKHLHNVGAW
                     AAWVPVALLMLMGLAAWARFGVANDFSPAALVPSTHLADVVFWSTLAFAFGGFETASL
                     MGGEIKDAARTVPRAVLLAGFAIVAIYVGGTLAVLVALPRSEVSGIQGIMQAIEHTAV
                     RVGLGPVVPVAAILVTVSSLGGVGAWLAAAGRLPYVAGIDRAAPSIFGKLHPKWGTPA
                     TALIVQSAVAGVFIVLGQAGTTVRAAYDVLVSMCIVCFFIPYVMMFAAMIRVQWFPAG
                     PEVRRPPGGRPVALAIGALGAVATTVSIGLACLPGANEPHPAVAVA"
     gene            complement(1311..1739)
                     /locus_tag="VFU45_06190"
     CDS             complement(1311..1739)
                     /locus_tag="VFU45_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358686.1"
                     /translation="MTEAEPGPERRLVPLLALLCGVMLLVDAAWAALARTFGWYHGPA
                     IAILSLTMYVVAGFLAFDCGGRLMAALAGAVVCIVDNSIGTLVTWAILPAPVARTLPS
                     VPDLAPLFAESVLLALGMGFIGGVLASLLRRRRQRARVSA"
     gene            complement(1744..2136)
                     /locus_tag="VFU45_06195"
     CDS             complement(1744..2136)
                     /locus_tag="VFU45_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358687.1"
                     /translation="MFPVLAMFAGAILLADVLFAIGEIRFGIDYDLVTWIAYPLYAIA
                     GYFAARCGGLALGALGGALTSLVDATIGWAVTWVMGPGRLPSGWPGFGFIAYVVGTVV
                     LYGTGLGIGGGLVHLAHAWWRGRRHAAA"
     gene            complement(2241..2642)
                     /locus_tag="VFU45_06200"
     CDS             complement(2241..2642)
                     /locus_tag="VFU45_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358688.1"
                     /translation="MPIAFEFDTEDHVVRLSASGVVTDADLIDTVEAMYADPRHRPGM
                     HELLDCLPVTRVGVTPRGVRAIAALISARLDRHRVGWRIAIVTRSATIFALARMYQLL
                     RDDSPEQVQVFRDLEPAEAWIVSTRSGVPVD"
     gene            complement(2724..2966)
                     /locus_tag="VFU45_06205"
     CDS             complement(2724..2966)
                     /locus_tag="VFU45_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358689.1"
                     /translation="MTGLLLAAGLLLAAVATWLLRPRRRPAAEPWETDVVETPDRDEL
                     ERAERQVRDRGRMREPDEEAPGDDWGPGATGATGGG"
     gene            complement(2963..4291)
                     /locus_tag="VFU45_06210"
     CDS             complement(2963..4291)
                     /locus_tag="VFU45_06210"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEU5358690.1"
                     /translation="MLVSKLIERKRDGGALTPGEWAELIAGYAHGDVPDYQMSALAMA
                     VVWRGLGPEELSALVDAMLASGDRLRFDGWATPRVDKHSTGGVGDKVSIVLAPMVAVC
                     GVAVPMMSGRGLGHTGGTVDKLESIPGFRVGLSLREAEEQTRRLGIAMLMASPEVARA
                     DQKLYGLRDVTGTVASVPLITASIMSKKLAEGLTGLVLDVKSGSGAFLTQLEQSLTLA
                     RTMIDVGESRGCRTVALVTAMDRPLGRACGNALEIEECIMALQGQGPEDLMEVTYALG
                     AEMLLVAGITRDRADARRRLEQALSSGKAAERFEAMIEAQGGNPGVVEDPSILPQANE
                     VEVFRAPAAGTVARVEPRRIGFGVIELGGGRTRKEDAIDPSVGFVITARPGDRVQGGE
                     PIASVFARDRAGIDRGLAVLAEAISFGPPEPLLPLVSHRLTKDGTEVLGG"
     gene            4336..5115
                     /locus_tag="VFU45_06215"
     CDS             4336..5115
                     /locus_tag="VFU45_06215"
                     /inference="COORDINATES: protein motif:HMM:NF045274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5358691.1"
                     /translation="MRSTAYALLAALCAFAVPAAAQTAADHIQMGRSVEDLDPQTGLK
                     HYREALALDSVNYEALWRASDALVDVGKQTPDSVASPARDSMYAEAEALARRAVQADS
                     MGAEGHFALAQAVGRASLTKSKKERVRRAKEIRDEALRAIALDPNHDGAYHVLGRWNA
                     EIMRLSGLTRFFAKTFLGGAVFNEASWDNAQKYMEKAVALNPNYVYHHLDLAEVLIDR
                     DRWAEARTQLEQVESLPVTDAMDPTYKATAKTLLARIADKK"
     gene            5197..5943
                     /locus_tag="VFU45_06220"
     CDS             5197..5943
                     /locus_tag="VFU45_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5358692.1"
                     /translation="MTDDIDTLKARIEALESERRQLLTVVEILEEIGGAAQFTDIVQA
                     VARRLGEALGLDRCSIFLAEKGGRSARLVASFEDPSIRNYLVDLDRYPELRRALETGQ
                     TVVIADAESDPGLQGVQKALKDRKVKSITVVPIKWRGAAIGAIFLRTFRDGQNFTDGD
                     IRFVEVVASLTSKALRNAHRIERLQQRLGGESAPLDPQQAVLAEFLRRLLTQAAARGG
                     HVSMAQATQEELDRLVGVTLTVLAQEADLH"
     gene            5949..>6141
                     /locus_tag="VFU45_06225"
     CDS             5949..>6141
                     /locus_tag="VFU45_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358693.1"
                     /translation="MATPAQRAAELRARIDRANRLYYVADAPEISDAEYDLLVRELQA
                     IERAHPELATPDSPTQRVGA"
BASE COUNT          993 a         2258 c         2154 g          736 t
ORIGIN      
        1 ggccaccgcc accgcggggt ggggctcatt ggcgccgggc aggcacgcga gcccgatcga
       61 cacggtggtg gcgacggccc cgagcgcgcc gatcgccagg gcgaccggcc ggccaccggg
      121 ggggcggcgc acctcgggac ctgcggggaa ccactggacc cggatcatcg cggcgaacat
      181 catgacgtac gggatgaaga agcagacgat gcacatgctg accaggacgt cataggcggc
      241 gcgtacggtg gtgcccgcct ggccgaggac gatgaagacg cccgccacgg ccgactggac
      301 gatgagcgcc gtggccggcg tgccccactt gggatgcagc ttgccgaaga tcgacggcgc
      361 cgcgcggtcg atgccggcca cgtacggcag ccgccccgcg gccgccagcc acgcccccac
      421 cccgcccagg ctgctgacgg tgaccaggat cgccgcgacg ggcacgaccg ggcccaggcc
      481 gacgcgcacc gcagtgtgct cgatggcctg catgatcccc tggatgccgc tcacctccga
      541 acgcggcagc gccaccagca ccgccagggt gccgccgacg tagatggcca cgatggcgaa
      601 gcccgccagc aggacggcgc gcggcacggt ccgggcggcg tccttgatct ccccgcccat
      661 gagcgacgcc gtctcgaacc cgccgaaggc gaacgcgagg gtggaccaga agacgacgtc
      721 ggcgaggtgg gtggacggga ccagggcggc cgggctgaaa tcgttggcga cgccgaagcg
      781 ggcccacgcc gccaggccca tcagcatgag gagcgccacc ggcacccagg cggcccaggc
      841 cccgacgttg tgcaggtgct tgccgacgtc gaggccgacg acattgagcc agcaggcgag
      901 cgccaggccg aagagcgaga acacgatgta gtacgtcgcg ctccccgaca gcccgatcca
      961 gtgcgaaccg ccgatgaaga gcgcgttccc cgccgcgaaa tagaggaggc ccgggaagta
     1021 caccaggttg ctggcccagt agagccagcc ggcgatgaac ccggcccgct cgccgaaggc
     1081 ctccttgacc cacaggtaca gcccgccctc ctccggatag cggctcgaca gctccagcac
     1141 cgtgaaggcg ctggggatga agatcgcccc gcacgcgatg gcccagatga cgagggagct
     1201 cggccccgcc gcggcggcgg ccgcgatcca gcgcaggccg accacggcaa cgatgaagaa
     1261 gagcacgacg tcgccgacgc cgaggacgcg ccgaagctgc cccggaccgg tcacgccgac
     1321 accctggccc gctggcgccg gcgtcggagc aggctggcca gcacgccgcc gatgaatccc
     1381 atcccgagcg ccagcagcac gctctccgcg aagagggggg ccaggtccgg gacggacggg
     1441 agggtccgcg ccaccggcgc gggaaggatg gcccaggtga cgagcgtccc gatggagttg
     1501 tcgacgatgc acacgacggc gccggcgagt gccgccatca gccggccgcc gcagtcaaag
     1561 gccaggaagc ccgccacgac gtacatggtg agcgacagga tggcgatcgc ggggccatga
     1621 taccacccga aggtccgggc gagcgccgcc cacgccgcat cgaccagcag catcacgccg
     1681 cacagcagcg ccaagagagg caccagccgg cgctcggggc ccggttcggc ctcggtcatc
     1741 gacttacgcc gccgcgtgcc ggcggccgcg ccaccaggcg tgggccaggt ggaccaggcc
     1801 gccgccgatg ccgaggccgg tgccatacaa cacgacggtg ccgaccacgt aggcgatgaa
     1861 cccgaacccg ggccagccgc tggggagccg gcccggcccc atcacccagg tcacggccca
     1921 gccgatcgtc gcgtcgacga gcgacgtgag ggcgcccccc agcgccccca gggccagccc
     1981 gccgcagcga gcggcgaagt accccgcgat ggcgtagagg gggtacgcga tccacgtgac
     2041 gagatcgtag tcgatgccga atcggatctc gccgatcgcg aacagcacat cggccaggag
     2101 gatggccccg gcgaacatcg ccaggaccgg gaacaatcgg cgggtgacca tcgggcaggg
     2161 acgcccctga atgcgagggt gacgctcagg aggggatcgc ataagatgga ggcgaagtcc
     2221 ctgcccgtcc actccccccg tcagtccacc gggaccccgc tccgggtcga gacgatccac
     2281 gcctcggccg gctccagatc ccggaatacc tgcacctgct ccggtgaatc atcccgcagg
     2341 agctggtaca tccgcgccag ggcgaagatg gtggcgctcc gggtcacgat ggcgatccgc
     2401 caccccaccc ggtgccggtc gaggcgggcg ctgatcagcg ccgcgatggc ccggacgccg
     2461 cggggcgtga cgcccacccg ggtcaccgga aggcagtcga gcaactcgtg catccccggc
     2521 cggtgccggg ggtcggcgta catggcctcg accgtgtcga tgaggtcggc gtccgtgacc
     2581 acgccggaag cggagagccg tacgacgtgg tcctccgtgt cgaactcaaa agcgatgggc
     2641 acgcgggatt cgggatcggg tgggaacggg cagtgcgcag gggccaactt atcacctgtc
     2701 ccgcccgggc gcggtgaccg gcctcacccc ccgccggtgg cgcccgtcgc tcccggcccc
     2761 cagtcgtccc cgggcgcttc ctcgtccggc tcgcgcatgc gcccccgatc gcgcacctgg
     2821 cgctcggccc gctcgagctc gtcccggtcg ggggtctcga ccacgtccgt ctcccacggc
     2881 tccgccgcgg gacgccggcg gggccgcagc agccaggtgg ccacggcggc gagcaggagg
     2941 ccggccgcca gcaggaggcc ggtcatccgc ccagcacctc ggtgccgtcc ttcgtgagcc
     3001 ggtggctcac cagcgggagc agcggctccg gcggaccgaa gctgatcgcc tccgccagga
     3061 ccgcgaggcc ccggtcgatc ccggcccggt cccgggcgaa tacgctcgcg atcggctcgc
     3121 caccctgcac ccggtccccc ggacgggcag tgatcacgaa gccgacgctc gggtcgatgg
     3181 cgtcttcctt gcgcgtccgc ccgccgccga gctcgatgac gccgaaaccg atgcgcctcg
     3241 gctccacccg cgccaccgtg ccggccgccg gcgcccggaa cacctcgacc tcgttggcct
     3301 gcggcaggat cgacggatcc tcgaccacgc cggggttccc tccctgcgcc tcgatcatcg
     3361 cctcgaagcg ctcagcggcc tttcccgagc tcagcgcctg ctccagccgt cggcgggcat
     3421 cggcgcggtc acgggtgatg ccggccacca ggagcatctc ggcgcccagg gcatacgtca
     3481 cctccatcag gtcctcgggg ccctggccct ggagcgccat gatgcattcc tcgatctcga
     3541 gcgcgttccc gcaggcgcgc ccgagcggcc ggtccatcgc ggtcacgagc gcgaccgtcc
     3601 ggcagccgcg cgattccccc acgtcgatca tggtccgcgc gagcgtgagc gactgctcca
     3661 gctgggtcag gaaggcgccg ctcccgctct tgacgtccag cacgagcccg gtgagcccct
     3721 cggccagctt cttggacatg atgctggcgg tgatgagcgg cacgctcgcc acggtgccgg
     3781 tcacgtcgcg cagcccgtag agcttctggt cggcccgcgc cacttcggga ctggccatca
     3841 gcatggcgat accgagccgt cgggtctgct cctcggcctc gcggagggag agccccaccc
     3901 ggaagcccgg gatcgactcg agcttgtcga cggtcccgcc ggtgtgcccc aagccgcgtc
     3961 ccgacatcat cggcacggcg acgccgcaga cggcgaccat cggcgccagg acgatcgaga
     4021 ccttgtcgcc cacgccgccg gtggagtgct tgtcgacgcg gggcgtggcc cagccgtcga
     4081 accggaggcg gtcgcccgag gcgagcatgg cgtccaccag ggcggagagc tcctccggcc
     4141 ccaggccgcg ccagacgacg gccatcgcca gcgccgacat ctggtagtcg gggacgtccc
     4201 cgtgcgcgta gccggcgatc agctccgccc actccccggg ggtcagggcg ccgccgtcgc
     4261 gcttgcgttc gatgagcttg gagacgagca ttagtgggtc atcccggtgt atgtgtcccg
     4321 agcgaggagt gccgaatgcg atcgaccgcc tatgcgctgc tggccgccct gtgcgcgttc
     4381 gccgtcccgg cggccgccca gaccgccgcg gaccacatcc agatgggccg gtcggtcgag
     4441 gacctcgatc cccagaccgg cctgaagcac taccgcgagg cgctggcgct cgacagcgtg
     4501 aattacgagg ccctgtggcg ggcgtcggac gccctggtgg atgtcggcaa gcagacgccg
     4561 gactcggtgg cgagcccggc ccgcgattca atgtacgccg aggccgaggc gctcgcacgc
     4621 cgggcggtgc aggccgattc gatgggggcg gagggccact tcgcgctggc gcaggcggtg
     4681 gggcgcgcct cgctcaccaa gagcaagaag gagcgggtcc ggcgcgccaa ggagatccgc
     4741 gacgaggcgc tccgcgcgat cgcgctcgac ccgaaccacg acggcgccta ccacgtcctg
     4801 ggccgctgga acgcggagat catgcggctg tcgggcctga cccggttctt cgccaagacg
     4861 ttcctgggcg gggcggtgtt caacgaggcc agctgggaca acgcccagaa gtacatggag
     4921 aaggccgtgg cgctcaatcc caactatgtc taccaccatc tggacctggc ggaggtcctg
     4981 atcgaccggg accgctgggc cgaggcacgc acccagctgg agcaggtcga gtcactgccc
     5041 gtgacggacg cgatggatcc gacctacaag gcgaccgcga agacgctcct ggcgcggatc
     5101 gcggacaaga agtagcgagt cacaggtcgt aagtcgcggg tcgcgcgtca cggcccgtga
     5161 cctgtgaccc gcgacccgga ccccaccgag acccgaatga ccgacgacat cgatacgctc
     5221 aaggcccgca tcgaggccct cgaatccgaa cgccgccagc tcctgacggt ggtggagatc
     5281 ctcgaggaga tcggcggcgc ggcccagttc accgacatcg tccaggcggt ggcccggcgc
     5341 ctgggcgagg ccctgggact cgaccgctgc tcgatcttcc tggccgagaa gggtggccgg
     5401 tcggcccggc tcgtggcgag cttcgaggac ccgagtatcc ggaactacct ggtcgacctc
     5461 gatcgctacc ccgagcttcg ccgcgcgctc gagaccggcc agacggtggt gatcgccgat
     5521 gcggagagcg accccgggct gcagggcgtc cagaaggcgc tcaaggaccg gaaggtcaag
     5581 agcatcaccg tggtgccgat caagtggcgg ggggccgcga tcggcgcgat cttcctccgc
     5641 accttccgcg acggccagaa cttcaccgac ggcgacatcc gcttcgtcga ggtcgtggcg
     5701 tcgctcacgt ccaaggcgct gaggaacgcg caccggatcg aacggctgca gcagcggctc
     5761 ggcggcgagt cggcgccctt ggacccccag caggccgtgc tggcggagtt cctccggcgg
     5821 ctcctgaccc aggcggcggc ccgcggcggc cacgtgtcga tggcccaggc cacccaggag
     5881 gagctcgacc gcctggtggg cgtgaccctc accgtgctcg cccaggaagc cgacctgcac
     5941 tgacccgcgt ggccaccccc gcccagcgcg cggcggagct tcgcgcccgc atcgaccgcg
     6001 ccaatcgcct ctattacgtc gccgacgcgc ccgagatctc ggacgccgaa tacgacctcc
     6061 tggtgcgcga gctccaggcg atcgaacgcg cccatccgga actcgccacg ccggactcgc
     6121 cgacccagcg cgtgggcgcc g
//
LOCUS       DASUAP010000240         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1355871, whole genome shotgun sequence.
ACCESSION   DASUAP010000240 DASUAP010000000
VERSION     DASUAP010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355871"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /gene="grpE"
                     /locus_tag="VFU45_06230"
     CDS             <1..352
                     /gene="grpE"
                     /locus_tag="VFU45_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU5358694.1"
                     /translation="TERAQGELLGRILDVVDDMDRLAAGGGRSPDVDQLHQAVVLTEK
                     KLWKELAALGFESVEPTGAPFDPSQHEALSTVPAPDAAQAHLVAQTFQVGYRLKGLLL
                     RPARVQVYGEPAQD"
     gene            354..1457
                     /locus_tag="VFU45_06235"
     CDS             354..1457
                     /locus_tag="VFU45_06235"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEU5358695.1"
                     /translation="MASKDLYQVLGVPDTADAAEIKKAYRRLAKQYHPDANPNNPAAA
                     ERFKEISEAHGVLSDAAKRKQYDQMRKYGAFSGRGPSAGGRPRGGTAGAGGYGGDGGE
                     AAFEFGDLGGLGDIFSSIFGRGRREEPVEAIETLVEIPFRVAALGGKVPVSLAVNEPC
                     ATCHGSGGAPGAKLTTCPECAGRGTVTFGQGGFAVSRPCPRCRGRGKLASEPCPTCHG
                     QGEVRTERRVTLTVPAGTESGAKVRLKGQGQPRAGGGAAGDLVVTFQVQPDRFFTRDG
                     LDLRCEVPINLAQAVFGTQLRVRTLDGKKVLLKVPAGTQPGRTFRVKGLGMEKGGRRG
                     DQLITVNVALPTDLSPEAAALLQQLAEKTGMRY"
     gene            complement(1454..>2273)
                     /locus_tag="VFU45_06240"
     CDS             complement(1454..>2273)
                     /locus_tag="VFU45_06240"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894183.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEU5358696.1"
                     /translation="VVYGHPALKTHAKAVLVIRREGDGVRHYVHIGTGNYNPKTARLY
                     TDFGLFSADPELAADLTDLFNVLTGFAQPAAYRKLIVAPRGMRERFLALIRREADHAR
                     AGRPARIVAKMNALVDPGIIAALYDASEAGAQVDLVVRGICCLRPGLPGVSERIRVVS
                     VVGRFLEHSRAFLFHNDGQEEAYIGSADWMPRNLDRRIEAVVPVTDPTHRATIRHVLT
                     LMLEDNRQAWDLGPDGDWTQRHPGPGEPERAAQRMLMEQARESGGRASGAVAAP"
BASE COUNT          329 a          782 c          853 g          309 t
ORIGIN      
        1 gaccgagcgg gcgcagggag aactgctcgg gcgcatcctc gacgtggtgg acgacatgga
       61 tcggctcgcg gcgggcgggg gccggagccc ggacgtggac cagctccacc aggccgtggt
      121 cctcaccgag aagaagctct ggaaggagct tgccgccctg gggttcgagt cggtcgagcc
      181 gaccggcgcg ccgttcgatc catcgcagca tgaggcgctc tcgaccgttc cggcgcctga
      241 cgcggcgcag gcgcacctgg tggcgcagac cttccaggtc gggtaccggc tcaaggggct
      301 cctcctccgg cccgcgcgag tacaggtcta cggcgagccg gcccaggact gacgtggcat
      361 ccaaggacct ctatcaggtg ctcggcgtgc ccgacaccgc cgacgccgcc gagatcaaga
      421 aggcgtatcg gcgtctggcc aagcagtacc atcccgacgc caatccgaac aatcccgccg
      481 ccgcggagcg cttcaaggag atctccgagg cgcacggcgt gctgtccgat gccgccaagc
      541 gcaagcaata cgaccagatg cggaagtacg gcgccttcag cgggcggggg ccgagtgcgg
      601 gggggaggcc gcgcggcggg acggccggcg cgggcgggta cggcggcgat ggcggcgaag
      661 ctgccttcga gttcggcgat ctcggcgggc tcggcgacat cttttcatcg atcttcggcc
      721 gggggcggcg cgaggagccg gtcgaggcga tcgagaccct cgtcgagatc cccttccgcg
      781 tggcggcgct gggagggaag gtcccggtct cgctcgcggt caacgagccc tgtgccacgt
      841 gtcacggcag cggcggcgcg cccggagcga agctcacgac ctgccccgaa tgcgccggtc
      901 ggggcaccgt gacgttcggc caggggggct tcgcggtcag ccggccctgc ccgcggtgtc
      961 gcggccgcgg gaagctcgcg tcggagccgt gtcccacgtg tcacgggcag ggggaggtcc
     1021 gcaccgagcg ccgcgtgacg ctcaccgtgc ccgcaggcac cgagtcgggc gccaaggtgc
     1081 gcctcaaggg acagggtcag ccccgggccg ggggaggcgc cgccggcgac ctcgtggtga
     1141 ccttccaggt ccagcccgac cggttcttca cccgggacgg gctcgacctg cgctgcgagg
     1201 tgccgatcaa cctggcgcag gcggtcttcg gcacccagct gcgggtccgc accctcgacg
     1261 gcaagaaggt gctgctcaag gtgccggccg ggacgcagcc gggacggacg ttccgggtga
     1321 aaggcctggg gatggaaaag ggcgggaggc ggggggacca gctcatcacc gtgaatgtgg
     1381 cactgccgac cgacctctcg ccggaggcgg ccgccctcct ccagcagctg gccgagaaga
     1441 ccgggatgcg gtactagggc gcggcgaccg ccccgctcgc ccggcccccc gattcacggg
     1501 cctgctccat cagcatccgc tgcgccgccc gctccggttc accgggaccc gggtgccgct
     1561 gcgtccagtc gccgtcgggc ccgaggtccc acgcctggcg gttgtcctcc agcatcagcg
     1621 tgagcacgtg acggatcgtg gcgcgatggg tcgggtcggt caccggcacc accgcctcga
     1681 ttcgtcggtc gaggttccgc ggcatccagt cggcggagcc gatgtaggcc tcctcctggc
     1741 cgtcgttgtg gaagaggaag gcgcgggagt gttcgaggaa gcgccccacc acgctcacca
     1801 cccggatccg ctcgctcacc cccggcagac ccggccgcag gcagcagatg ccccggacca
     1861 cgaggtcgac ctgggcgccg gcctccgacg cgtcgtagag cgccgcgatg atccccgggt
     1921 cgaccagggc gttcatcttg gcgacgatgc gtgccgggcg gccggcgcgg gcgtggtcgg
     1981 cctcccgtcg gatgagggcc aggaaccgct cgcgcatccc gcgcggtgcc acgatgagct
     2041 tgcggtatgc cgccggctgg gcaaagccgg tgagcacgtt gaagaggtcg gtcaggtcgg
     2101 ccgcgagctc gggatcggcc gagaagagcc cgaaatcggt gtagagccgc gccgtcttcg
     2161 ggttgtagtt gccggtcccg atgtggacgt agtggcgcac gccgtctccc tcgcggcgga
     2221 tgacgagcac ggccttggcg tgggtcttca gcgccggatg cccgtagacc acg
//
LOCUS       DASUAP010000241         4195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1246549, whole genome shotgun sequence.
ACCESSION   DASUAP010000241 DASUAP010000000
VERSION     DASUAP010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246549"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFU45_06245"
     CDS             <1..394
                     /locus_tag="VFU45_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358697.1"
                     /translation="PADGFGPAHDLALYAVESELGLRRGFFGLAAEGVAIGHLHIAPG
                     ASAAEEVVLADAVAGVLARESFGQMLSVEGFNFEVSAGVAARRSGVRAPELPEPLVEQ
                     LRTVYGSLRRRWADTPPHGALELSWDGA"
     gene            497..1003
                     /locus_tag="VFU45_06250"
     CDS             497..1003
                     /locus_tag="VFU45_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU5358698.1"
                     /translation="MAPLTLAPTDIFLRLGAATLAGAILGINRDLRGKPAGLRTHALV
                     TLSSAIAVVVMEEVALVAGQPGVDAESRVIQGLVTGIGFLGAGVILHAQGEVRGLTSA
                     ATVWFAAILGLAYGSAHWMVGTTGLALALIALLFGGSVEGWAHRIFGADRREGGRQDR
                     GAGSRLED"
     gene            1027..3276
                     /locus_tag="VFU45_06255"
     CDS             1027..3276
                     /locus_tag="VFU45_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5358699.1"
                     /translation="MLRRVLASVLLALSASSLSAQLPDTVSATLHRIFASAEFAPQRF
                     GPARWIDRGAAYTTLEKSATDSGAYDIVRYATASGERSVLVPARELTPAGDSTPLGID
                     DYAWSADGSRLLVFTNTRRVWRQNTRGDYWVLDRSAHTLRKLGGDGPPSTLMYAKFSP
                     QGDRVAYVRLGDLYVERLADGAITRLTTGADSLHVNGMSDWVYEEEFGLRDGFRWSPD
                     GTRIAFWHFDMTGVRTFLLINDTDSLYPYVIPIQYPKAGTTNSAVTAGVLNADGSGTT
                     TWLQVPGDPRDNYLPWMEWAGPKELLLQRIDRVQHDDALELADAATGAVRTVMTERDS
                     AWVDLEGEVPWLKDGQRFLWTSERDGWRHAYLVSRKDGSAQLVTKGDFDVIRVAALDE
                     AGGWLYYLASPDNATQAYLYRSRLNGKGKAERVTPADEAGTHFYSLSPDARWAFHSWS
                     TYDSPGGTDLVELPGHKVVRPLAPNTALAEKVRPWVRRPVEPFKVALSDGATLDGWMI
                     KPANFDSTKKYPILMYVYGEPAGQTVRDAWMGSARLWYQMIADQGYLVASVDNRGTPA
                     PKGRAWRKVVYKQIGVLSSAEQADAVRVLARTRPYVDPTRVAIWGWSGGGSSTLQAMF
                     RYPDVYQVGMSVAPVPDERLYDTIYQERYMGLPQVDGAAYDSASAIYQAQGLKGSLLL
                     VHGSGDDNVHFQGSQRLLNRLISLDKPIDFMEYPNRSHCICEGAGTTLHIYSLLTRYL
                     LTHLPAGGR"
     gene            3402..>4195
                     /locus_tag="VFU45_06260"
     CDS             3402..>4195
                     /locus_tag="VFU45_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5358700.1"
                     /translation="MSRMDRWMRRGAAYLLVAGASACVPGKPGPAPWTDTSPHRVSMV
                     TVAPGVRLEVLDWGGSGPPLVFLSGLQDVAHGFDDFAPRFTDHFHVLAITRRGYGASS
                     QPPSGYDLATRVADDKAVLDSLGLGTVALVGHSIAGDELTAFAGAYPERVSRLVYFDA
                     AYDHSQAGRLGKYFPPQPMTPADSASPAAVQAYTLRTFGMNIPEAQLRAIARYAPSGH
                     LLADVTPASVDSQVLAGCGHPDYAKVGAPALVIYNVVDSAPSFFPR"
BASE COUNT          610 a         1505 c         1461 g          619 t
ORIGIN      
        1 cccggcggac ggattcggcc cggcgcacga cctcgcgctc tacgccgtcg aatccgagct
       61 gggcctgcgg cgcggattct tcgggctcgc ggccgagggc gtcgccatcg gccacctgca
      121 catcgcgccg ggggccagcg ctgccgagga ggtcgtgctg gccgacgccg tggcgggggt
      181 gctggcccgg gagtcgttcg ggcagatgct gtcggtcgag ggattcaact tcgaggtgag
      241 cgccggtgtg gcggcccggc ggtcgggcgt acgggcgccc gagctgccgg aaccgctggt
      301 ggagcagctg cggaccgtgt acgggtcgct ccggcgccgc tgggccgaca cgccgccgca
      361 cggcgccctg gagctgagct gggacggcgc atgaccggcc gctcgaaggc ggaccttcag
      421 ccctgattca cgggctgttc acgcggcccg ttcgccattg ccgtccggcc cgacgcactg
      481 caccttagta gccatcatgg ctcccctcac gctcgccccg accgacatct tcctgcgcct
      541 cggcgcggcg accctggccg gcgcgatcct cgggatcaac cgcgacctcc gtggcaagcc
      601 ggcgggactc cgcacccacg ccctggtgac gctgagctcg gccatcgccg tcgtggtgat
      661 ggaggaggtg gcgctggtcg cggggcagcc gggggtcgac gccgagagtc gcgtcatcca
      721 gggcctcgtg accggcatcg gcttcctggg cgccggcgtc atcctgcacg cccaggggga
      781 ggtccgcggc cttaccagcg ccgccaccgt ctggttcgcc gcgatcctcg gcctcgccta
      841 cggcagcgcg cactggatgg tcggcaccac cggcctggcg ctggcgctca tcgccctcct
      901 cttcggcggc agcgtcgagg ggtgggccca ccgcatcttc ggcgccgacc gccgggaagg
      961 cggccgccag gaccgcggcg cgggcagccg cctcgaagac tgatccgtcc cactcccgga
     1021 gcccgcatgc ttcgtcgtgt cctcgccagc gtcctgctgg ccctctctgc gtcctccctc
     1081 tccgcccagc ttcccgacac cgtcagcgcc acgctccacc ggatcttcgc ctcggccgag
     1141 ttcgcgcccc agcgcttcgg cccggcgcgc tggatcgacc ggggcgcggc ctacaccacg
     1201 ctcgaaaagt cggccacgga ctccggcgcc tacgacatcg tgcgctacgc caccgcctcc
     1261 ggcgagcgga gcgtgctcgt tccggcccgg gagctcacgc ccgcggggga ctccactccg
     1321 ctcggcatcg acgactacgc ctggtccgcc gacgggagca ggctcctcgt cttcaccaac
     1381 acccgccggg tctggcggca gaacacccgc ggcgactact gggtcctcga tcgctccgcg
     1441 catacgctcc ggaagctcgg cggcgatgga cccccttcga cgctgatgta cgccaagttc
     1501 tcgcctcagg gtgaccgcgt ggcctacgtg cgcctgggcg acctctacgt cgagcgcctg
     1561 gccgacgggg ccatcacccg gctcacgacg ggtgcggact ccctgcacgt caacgggatg
     1621 agcgactggg tgtacgagga ggagttcggg ctgcgcgacg ggttccgctg gtcgcccgac
     1681 ggcacgcgga tcgcgttctg gcacttcgac atgaccgggg tgcggacctt cctgctcatc
     1741 aacgacaccg actcactcta cccgtacgtg atcccgatcc agtacccgaa ggcggggacc
     1801 accaattcgg cggtcaccgc cggggtgctc aacgccgacg ggagcggcac taccacctgg
     1861 ctccaggtcc ccggagaccc gcgcgacaac tacctcccct ggatggagtg ggccggaccg
     1921 aaggagctcc tgctgcagcg gatcgaccgg gtccagcacg acgacgcgct cgagctggcg
     1981 gacgccgcca ccggggcggt ccggaccgtg atgaccgaac gcgacagcgc ctgggtggac
     2041 cttgagggcg aggtcccgtg gctcaaggat ggccagcgct tcctctggac cagcgagcgc
     2101 gacggctggc gccacgccta tctcgtctcc cgcaaggacg ggtcggcgca actggtcacc
     2161 aagggggact tcgacgtcat ccgggtcgcg gcactcgacg aggcgggggg gtggctgtac
     2221 tacctggcct cccccgacaa cgccacccag gcgtatctct accgctcccg gttgaacgga
     2281 aaggggaagg ccgaacgggt gactccggcc gacgaggccg ggacgcactt ctattcgctc
     2341 tctcccgacg cgagatgggc gttccatagc tggtccacct acgactcccc gggcggcacc
     2401 gatctggtgg agctcccggg ccacaaggtc gtgcgcccgc tggcgcccaa caccgctctc
     2461 gccgagaagg tccggccgtg ggtcaggcgg ccggtcgagc cgttcaaggt ggcgctcagc
     2521 gacggcgcca cgctcgacgg gtggatgatc aagccggcca acttcgactc gacgaagaag
     2581 tacccgatcc tgatgtacgt ctacggagaa cccgccggcc agaccgtacg cgacgcctgg
     2641 atgggctccg cgcggctctg gtaccagatg atcgccgacc agggatacct cgtggcgagc
     2701 gtcgacaacc gcggcacgcc ggcgcccaag gggcgcgcgt ggcggaaggt ggtctacaag
     2761 cagatcggcg tgctgtcgag cgcggagcag gccgacgccg tgcgggtgct ggcgcgcacc
     2821 cgcccatatg tcgacccgac ccgggtggcc atctgggggt ggagcggcgg cggctcgagc
     2881 accctgcagg cgatgttccg gtatcccgac gtgtaccagg tggggatgag cgtggcgccg
     2941 gtgcccgacg aacggctcta cgacaccatc tatcaggagc gctacatggg gctcccccag
     3001 gtcgacggcg cggcgtacga ctccgcctcg gccatctacc aggcgcaggg gctcaagggc
     3061 tcgctcctcc tggtgcacgg ctcgggagac gacaacgtgc acttccaggg ttcccagcgg
     3121 ctcctcaatc ggctcatttc cctcgacaag ccgatcgact tcatggaata cccgaaccgg
     3181 agccactgca tctgcgaggg cgcagggacc acgctgcaca tctactcgct gctcacccgc
     3241 tacctgctga cgcacctgcc ggcggggggg cggtgaaggg gaaggcgcaa gaaagcggag
     3301 gaaagcgtgg gaaggcgcag gaaagcgggg gaaagcctga gagcgtagga aggcggaggg
     3361 gagtcggagg agtgcgtgaa cccaccgaac aagggtgtgt catgagccga atggaccgct
     3421 ggatgcgtcg cggggcagcg tacctcctcg tagctggcgc ttcggcgtgc gtcccgggca
     3481 agccggggcc ggcgccgtgg accgacacct ctccccaccg cgtctcgatg gtgacggtgg
     3541 cgccgggggt ccggctcgaa gtgctcgact ggggcgggtc ggggccaccg ctggtgttcc
     3601 tgtcggggct ccaggacgtg gcgcacggct tcgacgactt cgcgccccgg ttcacggatc
     3661 acttccacgt gctcgccatc actcgccgag ggtacggagc gtcgagccag ccgccgtcgg
     3721 ggtacgatct tgccacccgt gtggccgacg acaaggcggt gctcgactcc ctggggctcg
     3781 ggaccgtggc gctggtgggg cactcgatcg ccggcgacga gctcacggcg ttcgcgggcg
     3841 cctatccgga gcgcgtctcc cgcctggtgt acttcgacgc ggcctacgat cacagccagg
     3901 ccggccgtct gggcaagtac ttccccccgc aaccgatgac cccggcggac tcggcgtcgc
     3961 cggcggcggt gcaggcctac acgctccgca ccttcgggat gaacattccc gaggcgcagc
     4021 tccgggccat cgcccggtac gcgccctccg gccacctcct ggcggacgtg acgccggcgt
     4081 cggtcgactc acaggtcctg gccggatgcg ggcatccgga ctacgccaag gtcggggccc
     4141 ccgcgctcgt catctataat gtggtcgact cggcgccatc gttcttcccc cgctg
//
LOCUS       DASUAP010000242         4324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_238594, whole genome shotgun sequence.
ACCESSION   DASUAP010000242 DASUAP010000000
VERSION     DASUAP010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238594"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFU45_06265"
     CDS             <1..303
                     /locus_tag="VFU45_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5358701.1"
                     /translation="RDWDMTALAAIGHATERHLLRLFVDHAGVSPLNYLRAIRLERAR
                     QSLEWGASVSEAAAGAGFSSALQLRRAWSRQWGGSPRDTARAAGPSGRLAGPSGHP"
     gene            362..2596
                     /locus_tag="VFU45_06270"
     CDS             362..2596
                     /locus_tag="VFU45_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase"
                     /protein_id="HEU5358702.1"
                     /translation="MFHRTQRALVRGVTLALIAAALRAPAASAQVSTVDLRAAAALVA
                     RVVPGHAKGFRLSAIPDSAGLDVFEVAQDGRQVVLRGSSGVAIASALNWYLEHVAGVN
                     VSLPFQPIRLGTLPPVPAPVRIRTPYRIRYFFNYCTFSYSMAWWGWSDWQRMIDWMAL
                     KGINTPLAVTGQEAVWRATLLDLGFTRRQVADFLVGPAYLPWGWMGNIDGLGGPLPDS
                     WIDGHIALERRILARERSLGMRPVLQGFTGHVPASITKVFPAARIHQTGDWSAGFSGT
                     WFLDPLDPLFQRIGRRFIERQTELYGTDHLYAADPFNEIDPPSNDSTFLAGMGHAIYS
                     SMASADPQATWLLQGWFLYYQARFWQDPQAEAFLGAVPDDRMVVLDLWGDRHPVWQAR
                     RAFYGKPWVWNVLYNFGGKLSVNGDLPSIARNLDSALRSPAHGRLAGLGMMMEGLGTN
                     PIVPDFVLDQAWRDSVPDIEGWTHAYITRRYGADDAAAWHAWQVLLATAFRSSAQTGN
                     FLAERPQFYKAGTAYRSEPVAPYDVHLLARALDSLLAAAPALGQNDNYRYDVVNLTRQ
                     VLGQLAIPFVTEVQEAYARKDRAALTAAESRITTLLGDLDTLVATRQEFLLGRWTADA
                     RRWGHTPAERRLYEWNARNIITLWGTKCTEGQNDDLNLYAFKEWAGMFSSYFLPRWRE
                     FFARLDRSLVTGVPFDRAPFAVDMCTWEQRWSRAETAFPTEPRGDAVATARRLWDKYR
                     GDLR"
     gene            2684..2905
                     /locus_tag="VFU45_06275"
     CDS             2684..2905
                     /locus_tag="VFU45_06275"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEU5358703.1"
                     /translation="MKKNMGTADRTIRILAAIAVGVLYFMGKIGGTLAIVLGVIAVVF
                     VVTSFIGKCPAYVPLGISTDKEPPGPAGA"
     gene            3461..>4324
                     /locus_tag="VFU45_06280"
     CDS             3461..>4324
                     /locus_tag="VFU45_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5358704.1"
                     /translation="MPARPKKRAPRARAARPRRGTLAQRQSALLRLSAEVAAAIDEHE
                     VCRRVVAGLQDKALGYDFLGLFLLDATGDRVLQASVGWPDAPPMWRVHPGEGLSDRAI
                     KDGKLHYTPRVKEQKGYVASLASGSEVDVPLRVDDRTIGVLVVESAEPDAFASADFKV
                     LTAVAELASLAIGRARLLVSARRRADEQQALLDTMADLSGQLELSKLLHAVLGRAVAL
                     LGVTGGELAIYEEERRELVVVASTGISRDSTGTRLALGEGAMGKVAVSHEPLIIPNYD
                     QWAGRSTKYSDV"
BASE COUNT          625 a         1601 c         1434 g          664 t
ORIGIN      
        1 cgtgactggg acatgacggc gctggccgcg atcggccacg ccacggaacg ccacctgctc
       61 cgcctcttcg tcgaccacgc cggcgtctct cccctcaact acctccgggc gatccgcctg
      121 gaacgcgcgc gccagtcact cgagtggggc gcgagcgtca gcgaggcagc ggcgggcgcg
      181 gggttcagct ccgccctgca gctgcggcgg gcgtggagcc ggcaatgggg cggatccccg
      241 cgcgacacag cgcgggcggc gggccccagt ggacggctgg ccggcccatc gggccaccca
      301 tagctttggg gcaacgcccg ccatcccgtc acccgacgtc gaagtgcttc gaggtcccat
      361 cgtgttccat agaacccagc gcgccctcgt ccggggcgtt actctcgcgc tcatcgccgc
      421 tgcgctacgt gccccagccg catcggccca agtatccacc gtcgacctcc gcgccgccgc
      481 cgcgctcgtg gcccgggtgg tccccggcca cgccaagggc ttccggcttt ccgccatccc
      541 cgacagcgcg gggctcgacg tcttcgaggt ggcacaggac ggccgccagg tcgtcctccg
      601 aggatcctcc ggcgtggcga tcgcatcggc gctcaactgg tacctggagc atgtggcggg
      661 agtgaacgtc tcccttccct tccagccgat ccgcctcggg acgctccctc ccgtaccagc
      721 acccgtgcgt atccgcacgc cctaccgcat ccgctacttc ttcaactact gcaccttctc
      781 ctactcgatg gcgtggtggg gctggagcga ctggcagcgg atgatcgact ggatggcgct
      841 caaggggatc aatacccccc tcgccgtcac gggacaggaa gcggtctggc gggcgacgct
      901 cctcgatctc ggctttaccc gccggcaggt ggccgacttc ctcgtgggcc cggcctacct
      961 gccgtgggga tggatgggga acatcgacgg gctcggcggc cccctgcccg actcgtggat
     1021 cgacggccac atagcgctcg agcgcaggat cctcgcgcgc gagcgctcgc tcggtatgcg
     1081 gccggtgctg cagggtttca ccggccacgt gccggcgtcg atcaccaagg tcttccccgc
     1141 cgcccgcatc caccagaccg gcgactggtc cgccggcttc agcggcacct ggttcctcga
     1201 ccccctcgac ccgctcttcc agcgcatcgg ccggcgcttc atcgagcggc agacggagct
     1261 ctacggcacc gaccacctct acgccgccga tccgttcaat gagatcgatc caccgtccaa
     1321 cgactcgacc ttcctggccg ggatgggcca cgccatctac tcctcgatgg cgtcggcgga
     1381 cccgcaggcg acctggctcc tccagggatg gttcctctac taccaggcgc gcttctggca
     1441 ggatccacag gccgaggcgt tcctcggcgc ggtgccggat gaccggatgg tggtgctcga
     1501 cctctggggt gaccgccacc cggtgtggca ggcgcgccga gcgttctatg ggaagccgtg
     1561 ggtgtggaac gtgctctaca atttcggcgg caagttgagc gtcaacggcg acctcccctc
     1621 catcgcgcgg aacctcgaca gcgccctccg cagtcccgca cacggacgcc tcgccgggct
     1681 cggcatgatg atggaagggc tcggcaccaa cccgatcgtc cccgacttcg tgctggacca
     1741 ggcgtggcgc gacagcgtgc ccgacatcga agggtggacg cacgcctaca tcacccgtcg
     1801 ctacggcgcc gatgatgcgg cggcgtggca cgcctggcag gtgctcctgg ccaccgcgtt
     1861 ccgctcgagc gcccagaccg ggaacttcct ggccgagcgg ccgcagttct acaaggcggg
     1921 gacggcgtat cggtcggagc cggtggcgcc atacgacgtg cacctgctgg cccgggcgct
     1981 cgactccctg ctggccgcgg cgccggcact ggggcagaac gacaactatc gctacgacgt
     2041 ggtcaacctg acccggcagg tcctgggcca gctcgcgatc ccgttcgtga ccgaggtgca
     2101 ggaggcgtat gcccggaagg accgcgcggc tctgacggcg gcagagtccc gcatcaccac
     2161 cctcctcggc gacctcgaca ccctggtcgc cacgcggcag gagttcctgc tcggccgctg
     2221 gaccgccgat gcgcggcgct ggggacacac ccccgccgag cggcggctgt atgagtggaa
     2281 tgcacggaac atcatcacac tgtggggcac caagtgcacc gaggggcaga acgacgacct
     2341 caacctctac gcgttcaagg agtgggcggg gatgttctcg tcctacttcc tcccacggtg
     2401 gcgggagttc tttgcgcggc tcgaccggtc gctggtgacg ggggtcccgt tcgaccgggc
     2461 gccgttcgcg gtagatatgt gcacctggga gcagcgatgg tcgcgcgcgg aaacggcgtt
     2521 ccccaccgaa ccccggggag acgcggtggc gaccgcccgc cggctctggg acaagtatcg
     2581 gggtgacctg cggtagcggg tgctcccttg ccgggtcccg gaagggcccg caacttctgg
     2641 cggtcggcgt gggtggcaca accttgtcag cacggaggga atgatgaaga agaacatggg
     2701 tacggcggat cggacgatcc ggatcctggc agcgatcgcg gtcggcgtcc tctacttcat
     2761 gggcaagatc ggcggcacgc tcgccatcgt gctcggcgtc atcgcggtcg tgttcgtggt
     2821 caccagcttc atcgggaagt gcccggcgta cgtgccgctc ggcatctcga cggacaagga
     2881 gccgccgggc cccgccgggg cttaaggcgg gcaggaagcg agtagggcca cgaagggcgc
     2941 ggagtacacc gagagtcacg gagcactgca gattgtaatg gtcgaccgga aagtcgcccg
     3001 agaccacagc cggcaacgtg gtgccactct gtgcacctgg gctggtcacc cggcgaagcc
     3061 cttcagggcg ccctgccgag cggcggcttc agccgccagg agccgggcgg cgcatgcgcc
     3121 gcccctgcgg ttccgagctt gaagcgctct catcggctcg gctgggcgag gggcgcatcg
     3181 gagccattcc gttcaccctc accgatcaag cccttcaggg cgccctgccg agaggcggct
     3241 tcagccgccg ggaaccggac ggcgcatgcg ccgcccctgc ggatctgcat cgtcggagct
     3301 gtcccggtcg cccccatcgt ttgcagttct ccgtgcctct ccgcgccctc cgtgtcctcc
     3361 gtggtcccac cttcacactg cctccgctgg ccctccgccc cgacaagtcg catattcgag
     3421 gagccgtctc cagcccgtcg agtccggccc ccggagcccg atgcctgcac gtcccaagaa
     3481 gcgcgccccc cgcgcccgcg ccgcccgccc ccggcgtggc accctggcgc agcgccagtc
     3541 cgccctgctc cgcctctccg ctgaagtcgc cgccgccatc gacgagcacg aggtgtgtcg
     3601 ccgcgtcgtg gccggcctcc aggacaaggc gctcggctac gacttcctcg gcctcttcct
     3661 cctcgatgcc accggcgatc gcgtccttca ggcgagcgtc ggctggcccg acgccccgcc
     3721 gatgtggcgg gtccaccccg gcgagggcct gagcgaccgc gccatcaagg acggcaagct
     3781 ccactacacc ccgcgggtca aggaacagaa gggctacgtc gcgagcctcg cctcgggctc
     3841 cgaggtggac gttcccctcc gcgtggatga ccgcacgatc ggcgtgctgg tggtcgagag
     3901 cgccgaaccc gacgccttcg cctccgccga cttcaaggtg ctcacggccg tggccgagct
     3961 cgcgagcctc gccatcggcc gggcgcggct cctggtgagc gcgcgccgcc gggccgatga
     4021 gcagcaggcg ctcctcgaca ccatggccga cctctccggc cagctcgaac tctccaagct
     4081 cctccacgcc gtgctcggcc gcgccgtggc cctcctcggc gtgaccggcg gcgagctcgc
     4141 catctacgag gaggagcgcc gcgagctcgt ggtggtggcg agcaccggga tctcccgcga
     4201 ctcgaccggc acccgcctgg ccctgggcga aggggcgatg ggcaaggtcg ccgtctccca
     4261 cgagccgctc atcatcccca actacgacca gtgggccggc cgttcgacca agtactccga
     4321 cgtg
//
LOCUS       DASUAP010000243        14161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_184200, whole genome shotgun sequence.
ACCESSION   DASUAP010000243 DASUAP010000000
VERSION     DASUAP010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 14161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14161
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184200"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..717)
                     /locus_tag="VFU45_06285"
     CDS             complement(52..717)
                     /locus_tag="VFU45_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358705.1"
                     /translation="MRSILSMAACVLAAASLPHAARAQATQPLAPGVYFLWTPPGPQT
                     TSPENPVGPNFNWVAQQTGRPTVIGLSSPGQPPSLLAAGPVRSATGQAASREAPFLFG
                     RMWYRLVVSNGSVRAPDLWTSGTDYAAAGQPIALWLPNEGITADDEVFVRVRVDRSLA
                     TAAGTAPIYVRTSGPLPPGRFGSKVRVDLGPLPAGEYHVALEAWDTRTGMQNTSARTL
                     VVQ"
     gene            complement(801..1931)
                     /locus_tag="VFU45_06290"
     CDS             complement(801..1931)
                     /locus_tag="VFU45_06290"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU5358706.1"
                     /translation="MILPGATLGMLGGGQLGRMFTLTAREMGYKVLVLDPDPDSPAGR
                     VADLHLQRPYQDPGAIERMIQHCSAVTTEFENVPAEVLETLAVRLPVRPGAKAVAAAQ
                     DRLTEKAFLQRNGFATVGFYPLLSRADVEFGTNSVPGPWILKTARLGYDGKGQARVAT
                     PAEAQAAFDAHGGVPCILEQRVPLDLEVSVVLARGEDGRIAVFPPGENVHHHGILETT
                     TVPARLPGQYASHAAEIASAVAEALEYVGVLGVEMFLSGGQLLVNELAPRPHNSGHWT
                     QDAAASSQFEQQVRALCGLPLGDPRVLTPVTMINILGDVWEGGEPDWGAALADAGVKL
                     HLYGKTQPRPGRKMGHLNVLGGNAAETLHRAHRALRVLSRGR"
     gene            complement(1928..2395)
                     /gene="purE"
                     /locus_tag="VFU45_06295"
     CDS             complement(1928..2395)
                     /gene="purE"
                     /locus_tag="VFU45_06295"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU5358707.1"
                     /translation="MGSDSDWETLEHAAKTLAEFGVPFEHRVVSAHRTPDLLAEYARE
                     ARGRGLACIIAGAGGAAHLPGMLASHTTVPVLGVPVRSRALHGLDSLLSIVQMPAGIP
                     VATFAVGEAGARNAALFAVALLARSDAALQARLDDFRTRQTAKVLSTSLPPIK"
     gene            complement(2563..5223)
                     /gene="ppdK"
                     /locus_tag="VFU45_06300"
     CDS             complement(2563..5223)
                     /gene="ppdK"
                     /locus_tag="VFU45_06300"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEU5358708.1"
                     /translation="MTAEPKLVYFFGQGRAEGTAAMKDILGGKGAGLAEMTNIGIPVP
                     PGFTIASTMCLPYLESRSFPPRLEQQVGDALQRVEAATGKIFGSAEDPLLVSVRSGAA
                     VSMPGMMETILNLGLNDTTVSGVVKQTGNARFAWDSYRRFVQMYGAVVFDLPKAPFEQ
                     LLEAKKARRKVERDIDLPAEDLQALTAEYKAHIVQATGRPFPEEPMDQLWGAVTAVFE
                     SWNTRRAVDYRKLHAIPDAMGTAVSVCTMVFGNLGEDSGTGVAFSRDPSTGAATLYGE
                     YLLNAQGEDVVSGARTPQPIDRLAETLPQAHEELVRMARTLERHFRDVQDMEFTIERG
                     KLFMLQCRRAQRTGPAALRSACEMVEEGLISEEEAVARIPPNDLDQLLHPTIDASAAL
                     DLLTTGLPASPGAASGTIVFDADVAAERGRKGEAVILVRRETSPEDFHGMVAAKAILT
                     ARGGMTSHAAVVARGMGKPCVAGATDLDVNEADGTLKVRGRTLKAGDWITLDGATGRV
                     LAGQVRLSPPALNQYFDRVLGWADRLRHLRVRTNADTPADAHKARDFGAEGIGLCRTE
                     HMFFEGDRITAMREMIVAQDAPGRKAALAKLLPMQRGDFESIFRAMEGFPVTIRLLDP
                     PLHEFLPQEPEEIAHLARDLGKSAEEMTRIVHSLKEENPMLGLRGCRLGIVYPEITAM
                     QARAIFEAACTVAGRAGRVMPEVMIPLTASVVEFRQQALVVRQVAEEVFRERQLTVPY
                     LLGTMIELPRAALTADEIAREAQFFSFGTNDLTQTTWGLSRDDAGRFLPVYLERNIID
                     ADPFQILDQRGVGKLVEMACKLGRATRPDLKIGICGEHGGEPSSIAFCDALGMNYVSC
                     SPFRVPIARLAAAQAALAERGTMDRTRASL"
     gene            complement(5323..6702)
                     /gene="purF"
                     /locus_tag="VFU45_06305"
     CDS             complement(5323..6702)
                     /gene="purF"
                     /locus_tag="VFU45_06305"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU5358709.1"
                     /translation="MCGIFGVSGVPEAARLTYLGLYALQHRGQESAGIVAIDADGQAR
                     LHRGMGLVSDNFDDAELAKLRGDTAIGHTRYSTAGSSVLANAQPYLVNYRHGPLSIAH
                     NGNLTNAIELRRDLVERGSLFASTSDTEALVHLIARSDEPDPERQIRAALEAAEGAYT
                     LLITVGRAMYAVVDSRGFRPLMIGSLGAGIVVASESCALDLVGATPIRELRPGEFVRI
                     EDGKVTDLARLAPRPESRCVFELVYFARPDSRVFGESVDAVRRALGRQLAVEHPVPGA
                     DVVFSVPDSANAMALGYAEQSGVPLENGLIRNHYVGRTFINPTQALRVAKVRIKFNPV
                     REVIAGKKVVVVDDSLVRGTTSQGLVAMIRQAGAREVHLRLGSPPITGPCYYGIDTPT
                     REELIAATHSPEEIQRFLGVDSLGYLSLEGMLQAAGSRNRWCHACFSGEYPTGEGALV
                     SIGAVRGKR"
     gene            complement(6705..8999)
                     /gene="purL"
                     /locus_tag="VFU45_06310"
     CDS             complement(6705..8999)
                     /gene="purL"
                     /locus_tag="VFU45_06310"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEU5358710.1"
                     /translation="MTATAPTRAHPLAGEPPVTDALVREHNLTPFEFGRIQEILGRRP
                     TLTELGVFSALWSEHCSYKHSKPVLRKFPTSGPAVIQGPGENAGVLRLPRGWAVAFKV
                     ESHNHPSAVEPFQGAATGVGGILRDVFTMGARPVAVLNSLRFGPLEEPRNRYLFAGVV
                     RGVGTYGNCVGIPTLGGEVDFAPGYSGNPLVNAMAVGLLKEADLITARAHGEGNVLLA
                     VGARTGRDGIHGASFASEELSAKSEARRPQVQVGDPFTEKLLLEATLELRSRDLMVAI
                     QDMGAAGLTSSSAEMAARGGVGVELETDKVPVREAGMTPYEILLSESQERMLVIAEPR
                     RIAAIQEVCERWGLHATPIGHVTGDGMFRVRHGGTVVAEIPAQRLVDDAPVYHPEATE
                     GVQARARRARRPDRAPKLGVREALPFLLDLPSIASKRWVYEQYDSTVQANTVLGPGGD
                     AGVLRVDQEPFGLAVTLDCNARFVALDPYEGGKAVVAEAARNVACTGAVPLGITDCLN
                     FGSPEKPEVFFQFTEACRGISDACRAFDIPVTGGNVSFYNESPSGAIDPTPVVGLVGL
                     LDQVDQRIPSHFATPGDGIVIFGRTLGALGGSAYWAEVLDCAGGTPPAVDLAAERALQ
                     RLLADAARAGLLKSAHDCSTGGLAVALAEAAVGGPYAERGVGAEIDLREYAPGLSDAE
                     VLYAEDHGRVVATFALADAGRLAALARKHAVPGFGAGRVGAADGALRIQTAKGEYRWP
                     IAELRRAYFDAIPRRMALVTGGGT"
     gene            complement(9043..9513)
                     /locus_tag="VFU45_06315"
     CDS             complement(9043..9513)
                     /locus_tag="VFU45_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358711.1"
                     /translation="MRPVTITALALLVPALAFAQQPTTPPPSPAPTPAPVVVPPPTIP
                     GAVDTARPAAPALAPAPAAAPVSQQQVATISPGMTVAEVEAAWGKAGVTRTDGDQTFL
                     FYKNDCLKTCGTFDVVMLEGGKVVDAIVRASYHRYDGTSSSPAGTTPHFTRPPR"
     gene            complement(9513..10199)
                     /gene="purQ"
                     /locus_tag="VFU45_06320"
     CDS             complement(9513..10199)
                     /gene="purQ"
                     /locus_tag="VFU45_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU5358712.1"
                     /translation="MFRAAVIRFPGSNCDEDVLRAAARAGADAYFVWHQDTDLRAADA
                     VLLPGGFSFGDYLRSGAIARFSPVMQAVQRHAEAGGLVLGICNGFQVLCEAGLLPGAL
                     MKNARLTFASRPVTLRVERTATAFTSAYRPGQLIRMPVAHGDGRFTADAETLRRLEGE
                     GQVVVRYATAADNPNGSAADIAGICNASGTVVGLMPHPDRAADPVLGTTDGLGFFTSM
                     LAAVPAGGVR"
     gene            complement(10199..10450)
                     /gene="purS"
                     /locus_tag="VFU45_06325"
     CDS             complement(10199..10450)
                     /gene="purS"
                     /locus_tag="VFU45_06325"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU5358713.1"
                     /translation="MTFHVHVQVMPRAGLLDPQGQAVETALHALGFGGASEVRIGRSI
                     TFGLRAASAEDATTKARQMCDKLLANPVTEDFELRVEEA"
     gene            complement(10447..11307)
                     /gene="pssA"
                     /locus_tag="VFU45_06330"
     CDS             complement(10447..11307)
                     /gene="pssA"
                     /locus_tag="VFU45_06330"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEU5358714.1"
                     /translation="MDLMPRRRGLPRPNIGRAVIVLPSTFTLGNLFFGMWAIVAAYNR
                     NFVTAAWYVVFAAVLDMLDGRVARLSKTGTRFGAELDSLVDAISFGVAPAMIMYFLEF
                     STAGRFAWVICFAYVVAAVLRLARYNVQAEAKPAGSWFTGLPSPAAGMTLATWYPFSQ
                     TAFYRNVLEPMNLPHPGLTVLIVLAAVLMVSNVKYPRFPAAGFRSIKGIFGTVFTLTI
                     VVLGVTWPEYLFFPLGVMFMTYGVLRAGALALIERPEPSLAGAGPAQEDDDLTDDDDD
                     GAAARRENDL"
     gene            complement(11298..11924)
                     /locus_tag="VFU45_06335"
     CDS             complement(11298..11924)
                     /locus_tag="VFU45_06335"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEU5358715.1"
                     /translation="MRIAREGWPFILAFWALLLGFVLLHWWWSALLWGLVCLWVVAFF
                     RDPERTGPRGPQYVIAPADGLVVSIVDVDEPSFLHGKARRVAIFMNVFNVHVNRYPAD
                     GTVAYRHYNPGRFLNAADEKASLENEQSSIGLETPKGKMLVRQIAGLIARRIVTDHAP
                     GAAVRQGERLGLIRFGSRVDLFLPLAARVLVQVGQKTTAGTTVVAEWT"
     gene            complement(11924..12850)
                     /locus_tag="VFU45_06340"
     CDS             complement(11924..12850)
                     /locus_tag="VFU45_06340"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU5358716.1"
                     /translation="MSVVTLAESRLPLRVLRRGKVREVYEVDPEHLLLVASDRVSAFD
                     VVMREPVPWKGAVLTQISAFWFGLLADVVPSHFVTARTDEIVARIPMLLPHRAEILGR
                     ATLVRRTTPVPFECVVRGYLSGSAWGEYRARGTLAGETLPAGLVESDRLETPIFSPAT
                     KAESGHDENVPFTRVVESLGREVAERLRALSFAIYARGQAHAAQCGIIVADTKFEFGT
                     DSAGTLRLIDEVLTPDSSRFWPADRYAAGGPQPSFDKQPLRDYLAGLRQAGRWNGEAP
                     PPALPAEVIEATSVRYRDAFFRLTGRPLEESL"
     gene            complement(12847..>14161)
                     /gene="purB"
                     /locus_tag="VFU45_06345"
     CDS             complement(12847..>14161)
                     /gene="purB"
                     /locus_tag="VFU45_06345"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU5358717.1"
                     /translation="ESELGIPVPAGALAEMRAHLDDADLAAAARYERRFRHDVMAHVH
                     QFGDQAPAARPWLHLGATSAFVTDNADLIVLREGMQLILGRVLALLAALEVKARQTRA
                     LPCLAYTHFQPAQLTTVGKRITLWMQDFAIDAEDLGRRIASLRFRGCQGTTGTQASFL
                     ELFHGDHAKVRELERRVTAKLGFAEQFAVTGQTYPRKADSQVLDALSGVAQSAAKMAH
                     DLRLLQHEGELLEPFESEQIGSSAMAYKRNPMRAERIGALARFMISLQANGAHTAAAQ
                     WLERTLDDSANRRLTLPEAFLATDAILVLATNIAAGLEVRDAVIARHVREQMPFMATE
                     RWLMLAVEQGGDRQTLHEVIRRHSLTAAEQVAAGGANDLMERLSRDPAFSAIPVDRLS
                     AELEPSRYTGRAAEQVGEFLDEFLAPVLDRARPLATTAPMAEVQV"
BASE COUNT         2004 a         4929 c         5202 g         2026 t
ORIGIN      
        1 gcgggccggt tgccaggtgg caacgggccc gctcgcgtat tggggaccgc gttattgaac
       61 gacgagcgtg cgcgcgctcg tgttctgcat tcccgtgcgc gtatcccagg cctcgagcgc
      121 cacgtggtac tctccggccg ggagcgggcc gaggtcgacg cggaccttgg agccgaatcg
      181 tcccgggggc aacgggcccg aggtcctcac gtagatcggt gccgtcccgg ccgcggtcgc
      241 gagtgaccga tccacccgga cccgtacgaa cacttcatcg tccgcggtga tcccctcgtt
      301 cgggagccag agcgcgatcg gctggcccgc cgcggcgtag tcggtgcccg aggtccatag
      361 gtcgggcgcg cgaacgctcc cgttcgacac gacgagacgg taccacatcc ggccgaacag
      421 gaacggcgct tcccgcgagg ccgcctgtcc ggtcgccgat cgcaccggac ccgccgccag
      481 caggctgggg ggctgcccgg gcgatgacag tccgatgacg gtggggcgac cggtctgttg
      541 cgcgacccaa ttgaaattgg gcccgacagg attttccggg ctggtggtct gggggccggg
      601 aggggtccag aggaagtaaa cacccggcgc caatggctgt gtggcctggg cccgtgcggc
      661 gtggggaagc gacgcggcgg cgaggacgca cgccgccatc gacaggatcg aacgcacctt
      721 gcacccccag ggaggagagg atccggctcc tgagtgtacg ggaccgtggc ggggtgagtc
      781 aagcgcaccc cccatcccgg tcagcggccg cggctcagca cccggagcgc ccgatgcgcc
      841 cggtggaggg tctcggcggc gttccccccc aggacgttga ggtggcccat cttccggccc
      901 gggcggggct gggtctttcc atataggtgc agcttgaccc ccgcatcggc cagggcggcg
      961 ccccagtcgg gttctccccc ctcccagaca tcgcccagga tgttgatcat cgtgacgggg
     1021 gtgagcaccc gcgggtcgcc aagggggagt ccacagagcg cccgcacctg ctgctcgaac
     1081 tgggagctgg ccgccgcgtc ctgggtccag tgtccgctgt tgtgcggccg gggcgccagt
     1141 tcgttgacca ggagctgccc gccgctcagg aacatctcca ctcccagcac cccgacatac
     1201 tccagcgcct cggccaccgc actggcgatc tcggcggcgt gcgaggcgta ctgccccggg
     1261 aggcgtgcgg gcacggtggt cgtctccagg atgccgtggt gatgcacgtt ctctcccggc
     1321 gggaagaccg cgatgcggcc gtcctctccc cgggcgagca ccaccgacac ctcgaggtcg
     1381 agcgggaccc gctgctccag gatgcagggg acgccgccgt gcgcatcgaa cgccgcctga
     1441 gcctcggcgg gggtggcgac acgcgcctgg cccttgccgt cgtaaccgag ccgggcggtc
     1501 ttgaggatcc agggccccgg caccgaattg gtgccgaact cgacgtcggc ccgcgagagg
     1561 agcggataga acccgaccgt cgcgaagccg ttccgctgga ggaaggcctt ctcggtgagg
     1621 cggtcctggg ccgccgccac ggccttggcg ccgggccgca ccggcaggcg caccgcgagg
     1681 gtctccagca cctcggccgg gacgttctcg aactcggtcg tcaccgcgct gcagtgctgg
     1741 atcatccgct cgatcgcacc cgggtcctgg tacgggcgct gcaggtgcag gtcggcgacg
     1801 cggccggcgg ggctgtccgg gtcgggatcc aggaccagga ccttgtaccc catctcgcgg
     1861 gcggtgagcg tgaacatccg cccgagctgc ccgccgccga gcatgcccag ggtggctccg
     1921 ggaaggatca cttgatcggg gggagggagg tggagaggac tttggccgtc tgccgggtcc
     1981 ggaagtcgtc gagccgggcc tggagcgcgg cgtccgaacg ggccaggagc gccacggcga
     2041 agagcgcggc attccgggcg ccggcctcac cgacggcgaa cgtggcgacc gggatgccgg
     2101 cgggcatctg gacgatcgac aggagcgaat cgagcccgtg gagcgcccgc gaccggaccg
     2161 gcacgcccag caccggcacg gtggtgtgcg aggcgagcat ccccggcagg tgggcggcgc
     2221 cgcccgcgcc ggcgatgatg caggcgaggc cgcgtccccg cgcctcgcgg gcatattccg
     2281 ccagcaggtc gggagtgcgg tgggcggaga cgacccggtg ctcgaacggg acgccgaact
     2341 ccgccagcgt cttggccgcg tgttcgagcg tctcccagtc ggagtcgctt cccatcacta
     2401 cgcctaccag gggtgcgggc attcggggtc cggtggaagg tcgggggacg gctatcggct
     2461 ctcagctctc agctctcggc tctcagctct cagctctcag ttctcagctc tcagttcacg
     2521 aacggtcgga tggtttcaac agcggccacg ggctgaccgc gtctacaggc tcgcccgcgt
     2581 ccggtccatc gttccgcgct cggcgagggc ggcctgcgcc gcggcgagcc gggcgatggg
     2641 cacccggaac ggtgagcagc tcacgtagtt catccccagg gcgtcgcaga aggcgatcga
     2701 actcggctcg cctccgtgct cgccgcagat cccgatcttg aggtcggggc gggtcgcgcg
     2761 gccgagcttg caggccatct cgaccagctt gccgacgccc cgctggtcga ggatctggaa
     2821 cgggtcggcg tcgatgatgt tccgctccag gtacaccggg aggaaccgtc cggcgtcgtc
     2881 gcgggagagg ccccaggtgg tctgggtcag gtcgttggtg ccgaaggaga agaactgcgc
     2941 ctcccgggcg atctcgtcgg cggtgagggc ggcgcggggg agctcgatca tcgtcccgag
     3001 gaggtacggc accgtgagct gccgctcgcg gaagacctcc tcggccacct gccggaccac
     3061 gagggcctgc tggcggaatt ccaccacgct ggcggtgagg gggatcatca cctcgggcat
     3121 cacccggccg gcccggcccg ccacggtgca ggcggcctcg aagatggcgc gggcctgcat
     3181 ggcggtgatc tcggggtaga cgatcccgag ccggcacccg cggagcccca gcatcgggtt
     3241 ctcctccttg agcgaatgca cgatgcgggt catctcctcg gccgactttc cgaggtcccg
     3301 ggccaggtgg gcgatctcct cgggctcctg gggcaggaac tcatgcaggg gcgggtcgag
     3361 gagccggatg gtgacgggaa atccttccat cgcccggaag atcgactcga agtcgccccg
     3421 ctgcatcggg agcagcttgg cgagcgccgc cttccggccc ggggcgtcct gcgccacgat
     3481 catctcgcgc atcgcggtga tgcggtcgcc ctcgaagaac atgtgctcgg tgcggcagag
     3541 cccgatcccc tcggcgccga agtcccgggc cttgtgggcg tccgccgggg tgtcggcgtt
     3601 ggtgcggacc cgcaggtgcc gcagccggtc ggcccagccc agcacccggt cgaagtactg
     3661 gttgagggcc gggggcgaga gccggacctg gcccgcgagg acccggcccg tggcaccgtc
     3721 gagggtgatc cagtcccccg ccttgagcgt gcgcccgcgg accttcagcg tcccgtccgc
     3781 ctcgttcacg tcgaggtcgg tggcgccggc cacgcacggc ttccccattc cgcgggccac
     3841 caccgccgcg tggctcgtca tcccgccccg tgcggtgagg atggccttcg ccgccaccat
     3901 cccgtggaag tcctcggggc tcgtctcccg ccggacgagg atgaccgcct cgcccttgcg
     3961 accccgctcg gcggccacgt cggcgtcgaa gacgatcgtg cccgaggcgg cgccgggaga
     4021 ggcggggagc cctgtggtca ggaggtcgag cgccgcgctg gcgtcgatgg tcgggtggag
     4081 cagctggtcc aggtcgttcg gagggatgcg ggcgacggcc tcctcctcgc tgatgagccc
     4141 ttcctcgacc atctcgcagg cgctgcggag cgccgcgggt ccggtccgct gggctcgccg
     4201 gcactgcagc atgaagagct tcccgcgctc gatggtgaat tccatgtcct gcacgtcgcg
     4261 gaaatgacgc tccagcgtcc gtgccatccg gaccagctcc tcgtgggcct gggggagggt
     4321 ctcggcgagc cggtcgatcg gttgcggcgt gcgcgcgccc gagaccacgt cctctccctg
     4381 ggcgttgagg aggtattccc cgtagagcgt ggcggcgccg gtggacgggt cgcggctgaa
     4441 ggcgacgccg gtgccggaat cctcgcccag gttgccgaag accatcgtgc acacgctgac
     4501 cgcggtgccc atcgcgtcgg ggatggcgtg gagcttccga tagtccaccg cgcggcgggt
     4561 gttccagctc tcgaagacgg cagtgaccgc gccccagagc tggtccatcg gctcctcggg
     4621 gaatggtcgc ccggtggcct ggacgatgtg cgccttgtac tccgccgtga gcgcctgcag
     4681 gtcctcggcc ggaaggtcga tgtcgcgctc gaccttccgc ctggccttct tggcctccag
     4741 caactgctcg aagggcgcct tgggcaggtc gaacacgacc gcgccgtaca tctggacgaa
     4801 ccggcggtag gaatcccagg cgaagcgcgc gttgccggtc tgcttgacca ccccgctgac
     4861 ggtggtgtcg ttgagcccga ggttgaggat cgtctccatc atccccggca tcgacacggc
     4921 ggcccccgac cggaccgaga cgaggagggg atcctccgcc gacccgaaga tcttgccggt
     4981 ggcggcctcg acccgctgca gcgcatcgcc gacctgctgc tcgagccggg gcgggaagct
     5041 gcggctttcg aggtagggga ggcacatcgt gctcgcgatg gtgaaccccg gtggcaccgg
     5101 gatcccgatg ttggtcatct cggcgagacc ggcacccttg ccgccgagga tgtccttcat
     5161 cgcggcggtc ccttcggcac gcccctgccc gaagaagtag accagcttgg gttcggccgt
     5221 catttcacgc ccccggggac cgcgttgcgc ggaagcttca tgtgtcgcac cttctccacc
     5281 gtacccctta cctctcacct cttacctctc accgcttacc tctcacctct tacctctcac
     5341 cgctccgatg ctcaccagcg caccctcgcc ggtcgggtac tcgcccgaaa agcaggcgtg
     5401 gcaccagcga ttccgcgagc ctgccgcctg gagcatcccc tcgagcgaga gatagccgag
     5461 ggagtcgaca ccgaggaacc gctggatctc ctcgggggaa tgggtcgcgg cgatgagctc
     5521 ctcgcgggtc ggggtgtcga tgccgtagta acaggggccg gtgatgggcg gcgatccgag
     5581 ccgcaggtgc acctcccgtg cccccgcctg ccggatcatc gccaccagcc cctggctggt
     5641 ggtcccccgg accaggctgt cgtcgaccac gaccaccttc ttgccggcga tcacctcgcg
     5701 caccgggttg aacttgatcc ggaccttggc cacccggagg gcctgggtgg ggttgatgaa
     5761 ggtccggccg acataatggt tgcggatgag cccgttctcg agcggcacgc ccgactgctc
     5821 ggcgtagccc agcgccatcg cgttggcgct gtccggcacg ctgaagacca cgtcggcgcc
     5881 ggggaccggg tgctccacgg ccagctggcg cccgagcgcc cgacgcacgg cgtcgaccga
     5941 ctcgccgaag acccggctgt cgggccgcgc gaagtagacg agctcgaaca cgcaccggct
     6001 ttccggccgg ggcgccaggc gcgccaggtc ggtgaccttc ccgtcctcga tgcgcacgaa
     6061 ctcgccgggg cggagttcgc ggatgggcgt ggcgccgacc aggtcgagcg cgcagctctc
     6121 cgaggccacc acgatcccgg cgccgaggct gccgatcatc agcggccgga acccgcgcga
     6181 gtcgaccacc gcgtacatgg cccggcccac ggtgatgagc agggtgtagg ccccttcggc
     6241 cgcttccagc gcggcgcgga tctggcgttc ggggtcgggc tcgtccgatc gcgcgatcag
     6301 gtggacgagg gcctcggtgt cgctggtcga cgcgaagagc gatccgcgct cgaccaggtc
     6361 gcgccgcagc tcgatggcgt tggtcaagtt gccgttgtgg gcgatgctga gcgggccgtg
     6421 ccggtagttg acgaggtacg gctgggcgtt ggccagcacc gaactgccgg cggtcgaata
     6481 gcgggtgtgg ccgatggcgg tgtcgccccg gagcttggca agctcagcgt cgtcgaagtt
     6541 gtcggagacg aggcccatcc cccggtggag ccgggcctgc ccgtccgcat cgatcgccac
     6601 gatgcccgcg ctctcctggc cccggtgctg cagggcgtag aggccgaggt aggtcagccg
     6661 cgccgcctcg gggacaccgc tcacaccgaa gatgccgcac atggtcaggt ccccccgccg
     6721 gtcacgagtg ccatccggcg cgggatcgcg tcgaagtagg cgcggcgcag ctcggcgatg
     6781 ggccagcgat actcgccctt cgcggtttgg atccggagcg ccccgtcggc ggccccgacc
     6841 cgcccggcgc cgaacccggg cactgcatgc ttccgggcca gcgccgcgag gcgccccgcg
     6901 tcggccagcg cgaacgtggc caccacccgc ccgtggtcct cggcgtacag cacttcggca
     6961 tcggagagcc ccggggcgta ctcccgcagg tcgatctcgg cgcccacgcc gcgctcagcg
     7021 tagggtccgc cgacggccgc ctcggcaagg gccaccgcga ggccgccggt cgagcaatcg
     7081 tgcgccgact tgaggagccc cgcccgcgcg gcgtccgcca ggaggcgctg cagcgcgcgc
     7141 tcggccgcca ggtcgaccgc agggggcgtt ccgcccgcgc agtcgagcac ctcggcccag
     7201 tacgccgacc cgcccagcgc gcccagggtc cgcccgaaga tcacgatgcc atcgccggga
     7261 gtggcaaaat ggctcgggat ccgctggtcc acctggtcga gcagtcccac cagtccgacg
     7321 acaggggtcg gatcgatggc gccgctcggg ctctcgttgt agaacgagac gttgccgccg
     7381 gtcaccggga tgtcgaaggc gcggcacgcg tcgctgatgc cgcggcaggc ctcggtgaac
     7441 tggaagaaga cctcgggctt ctcggggctg ccgaagttga ggcagtcggt gatgccgagc
     7501 ggcacggcgc cggtgcaggc cacgttgcgg gccgcctccg ccaccaccgc cttgcctccc
     7561 tcgtagggat cgagcgccac gaaccgcgcg ttgcagtcga gtgtgaccgc aaggccgaac
     7621 ggctcctggt ccacccggag cacgccggcg tcgccgccgg ggccaaggac cgtgttggcc
     7681 tggacggtgg aatcgtactg ttcgtagacc caccgcttgc tcgcgatgct cgggaggtcg
     7741 agcaggaacg ggagcgcctc gcgcacgccg agcttcggcg cccggtccgg ccggcgggcg
     7801 cggcgggcgc gggcctggac gccttccgtg gcctccgggt gatacaccgg tgcgtcgtcc
     7861 accagccgct gggccgggat ctccgccacg acggtgccgc cgtggcgcac gcggaacatc
     7921 ccgtcgcccg tcacgtggcc gatcggcgtc gcgtgcagcc cccagcgttc gcagacctcc
     7981 tggatcgccg cgatgcggcg gggctcggcg atcaccagca tccgctcctg cgactcggac
     8041 aggaggatct cgtagggcgt catcccggcc tcgcgcaccg ggaccttgtc ggtctcgagt
     8101 tcgacgccga cgccgccccg ggccgccatt tccgcggagg aacttgtcag gccggccgcg
     8161 cccatgtcct ggatggccac catcaggtcg cgcgagcgga gttcgagggt ggcctcgagg
     8221 aggagcttct cggtgaaggg gtcgcccacc tggacctggg ggcggcgcgc ctccgacttg
     8281 gccgagagct cctccgaggc gaagctggcg ccgtggatcc cgtcgcgtcc ggtgcgcgca
     8341 ccgacggcga gcaggacgtt gccctccccg tgggcgcggg cggtgatgag gtcggcttcc
     8401 ttgaggagcc cgaccgccat cgcgttgacc agcgggttgc cgctgtagcc gggcgcgaag
     8461 tcgacctcgc cgccgagcgt cgggatgccg acgcagttgc cgtaggtgcc gacaccgcgc
     8521 accaccccgg caaagaggta ccggttgcgg ggctcctcga gcgggccgaa gcgcaggctg
     8581 ttgaggaccg ccaccgggcg cgcacccatc gtgaacacgt cccggaggat gccgccgact
     8641 ccggtggcgg cgccctggaa cggctccacc gccgaggggt ggttgtgcga ttccaccttg
     8701 aatgccaccg cccagccgcg cggcagccgg aggaccccgg cgttctcgcc gggtccctgg
     8761 atgacggcgg ggccgctggt ggggaacttc cggagcaccg gcttggaatg cttgtaggag
     8821 cagtgctccg accagagcgc ggagaagacg ccgagttcgg tcagggtcgg ccggcgcccg
     8881 aggatctcct ggatccgccc gaactcgaac ggcgtgaggt tgtgctcgcg gacgagtgcg
     8941 tccgtcaccg gcggctcacc ggcgaggggg tgggcgcggg tcggtgcggt cgcggtcact
     9001 cggggcggcc ccagtcggta cgggtcaggt gaaggtggcg ggtcaccgcg ggggccgggt
     9061 gaagtgcggg gtggtgccgg ccggggacga cgacgtgcca tcgtagcggt ggtagctggc
     9121 gcggacgatc gcgtccacca ccttgccgcc ctccagcatc accacgtcga aggtgccgca
     9181 ggtcttgagg cagtcgttct tgtagaagag gaacgtctgg tcgccatcgg tgcgggtgac
     9241 gccggccttg ccccaggccg cctcgacctc ggcgacggtc atcccggggg agatcgtggc
     9301 gacctgctgc tgggacaccg gcgccgctgc cggggcgggt gccagggccg gtgcggcggg
     9361 cctggccgtg tccaccgcgc cgggaatggt gggcggcggg accaccacgg gcgccggcgt
     9421 cggtgcgggg ctcggcggcg gcgtggtcgg ctgctgggcg aacgccaggg cggggaccaa
     9481 cagggcgagg gcggtgatgg tgaccgggcg catcagcgga ctcctcccgc ggggactgcc
     9541 gcgagcattg aggtgaagaa gccaagccca tcggtggtgc cgagtacggg gtcggccgca
     9601 cgatccgggt ggggcatcaa tcccaccacc gtgccggagg cgttgcagat cccggcgatg
     9661 tccgcggccg atccgttcgg attgtcggcc gcggtggcgt accgcaccac cacctggccc
     9721 tcgccttcca gccgacgcag cgtctcggcg tccgccgtga agcgcccgtc gccgtgggcc
     9781 accggcatcc ggatgagctg gcccgggcgg taggcgctgg taaaggcggt ggcggtccgt
     9841 tccacccgga gcgtgaccgg gcgggacgcg aaggtgagcc gcgcattctt catcagcgcg
     9901 ccggggagga gccctgcctc gcagagcacc tggaacccgt tgcagatgcc gagcaccaac
     9961 ccgcccgctt cggcgtggcg ctggaccgcc tgcatcaccg ggctgaaccg cgcgatggcg
    10021 ccggagcgga ggtagtcgcc gaagctgaag ccgccgggga ggagcaccgc gtcggcggcg
    10081 cgcaggtcgg tgtcctggtg ccacacgaag taggcgtccg cccccgcacg cgcggcggcc
    10141 cggagcacgt cctcatcgca gttgctcccg gggaagcgga tcacggccgc ccggaacatc
    10201 aggcctcctc cacgcggagc tcgaagtcct cggtcaccgg gttggccagg agcttgtcgc
    10261 acatctgccg ggccttggtg gtggcgtcct cggcggaggc ggcgcggagc ccgaaggtga
    10321 tcgagcggcc gatccgcacc tcggaggcgc cgccaaagcc gagggcgtgg agcgccgtct
    10381 cgaccgcctg cccctgcggg tcgaggaggc cggcgcgggg catcacctgc acatgcacgt
    10441 ggaaggtcac aggtcgttct cccggcgcgc ggcggcgcca tcatcgtcgt cgtcggtcag
    10501 gtcgtcgtcc tcctgcgccg gaccggcgcc cgcgagcgac ggttcagggc gttcgatgag
    10561 cgccagcgcc ccggcccgga gcacgccgta cgtcatgaac atgacgccga gcgggaagaa
    10621 gaggtattcg ggccaggtga cgccgaggac gacgatggtc agcgtgaaga ccgtgccgaa
    10681 gatccccttg atgctccgga acccggcggc ggggaagcgg gggtacttca cgttgctcac
    10741 catcagcacc gcggcgagca cgatcaggac cgtgagtccc gggtggggaa ggttcatcgg
    10801 ctccagcacg ttccgataga acgccgtctg gctgaacgga taccaggtgg cgagcgtcat
    10861 ccccgccgcc ggggacggca gccccgtgaa ccagctgccg gcgggcttgg cctcggcctg
    10921 cacgttgtag cgcgccaggc gcagcaccgc cgccacgacg tacgcgaagc agatgaccca
    10981 ggcgaatcgc ccggcggtgg agaactccag gaagtacatg atcatcgccg gcgcgacgcc
    11041 gaaggagatg gcgtccacca gcgagtcgag ctcggcgccg aagcgggtgc ccgtcttgga
    11101 gagccgggcc acccgcccgt cgagcatgtc cagcacggcg gcgaacacca cgtaccacgc
    11161 cgccgtgacg aagttccggt tgtaggccgc gacgatggcc cacatcccga agaacaggtt
    11221 gccgagcgtg aacgtgctgg ggagcacgat caccgcccgg ccgatgttgg ggcggggcag
    11281 gccgcggcgc cggggcatca ggtccattcg gccaccaccg tggtccccgc cgtggtcttc
    11341 tggccgacct gcaccagcac ccgggcggcg aggggaagga agaggtcgac ccgggagccg
    11401 aagcggatca ggcccagtcg ctcgccctgg cgcaccgcgg cgccgggcgc gtggtcggtc
    11461 acgatccgcc gggcgatgag gccggcgatc tggcggacga gcatcttgcc cttcggcgtc
    11521 tccaggccga tcgacgactg ctcgttctcg aggctcgcct tctcgtcggc ggcgttgagg
    11581 aagcgtcccg ggttgtagtg ccggtacgcc acggtgccgt cggccggata ccggttgacg
    11641 tgcacgttga acacgttcat gaagatcgcc acgcggcgcg ccttgccgtg aaggaagctc
    11701 ggctcgtcga cgtcgacgat gctcacgacg agaccgtcgg ccggcgcgat gacgtactgc
    11761 gggccgcgcg ggccggtgcg ctccgggtcg cggaagaagg ccaccaccca gaggcacacg
    11821 agcccccaga ggagagcgga ccaccaccag tgcaggagca cgaacccgag gagcagggcc
    11881 cagaacgcca ggatgaacgg ccagccttcg cgcgcgatgc gcatcagagc gactcctcca
    11941 gcggccggcc cgtgagccgg aagaaggcgt cccgatagcg gacgctcgtg gcctcgatga
    12001 cctcggccgg gagggccggc ggcggcgcct cgccgttcca ccggcccgcc tggcggagcc
    12061 cggccaggta gtcgcgcagc ggctgcttgt cgaagctcgg ctgcggcccg ccggcggcgt
    12121 agcggtcggc cggccagaag cgcgagctgt ccggcgtgag cacctcgtcg atcagccgga
    12181 gcgtcccggc cgagtcggtg ccgaactcga acttggtgtc ggcgacgatg atgccgcact
    12241 gcgccgcgtg ggcctggccg cgggcgtaga tggcgaagct cagggcccgc agacgttcgg
    12301 ccacctcgcg gccgagcgac tcgaccaccc gcgtgaaggg gacgttctcg tcgtgcccgc
    12361 tctccgcctt cgtggcgggg gagaagatcg gcgtctcgag ccggtcgctc tccaccaggc
    12421 ccgcaggaag cgtctcgccg gccagcgttc cccgggcgcg gtactcgccc caggcggaac
    12481 cggagaggta gccccgcacc acgcactcga aggggaccgg cgtcgtccga cgcaccagcg
    12541 tggcgcgccc caggatctcc gcccggtgcg gcagcagcat cgggatgcgg gcgacgatct
    12601 cgtcggtccg cgccgtcacg aaatgtgagg gcacgacatc ggccaggagc ccgaaccaga
    12661 aggcgctgat ctgggtgagg acggcgccct tccacggcac cggctcgcgc atcaccacgt
    12721 cgaacgcgct cacccggtcg ctcgccacga gcagcaggtg ctcggggtcg acctcgtaga
    12781 cctcccggac cttcccgcgc cggaggaccc ggagcggcag gcggctctcc gccagggtca
    12841 ccacgctcat acctgcacct ccgccatcgg cgccgtcgtg gcgagtggcc gcgcccggtc
    12901 gaggactggc gccaggaact cgtcgaggaa ttcccccacc tgctcggccg cccggccggt
    12961 gtagcgggac ggctcgagct cggccgagag ccggtcgacc gggatcgccg agaacgccgg
    13021 atcgcgtgac aggcgctcca tcaggtcgtt ggcaccgccc gccgccacct gctcggctgc
    13081 ggtgagcgaa tggcggcgga tgacctcgtg cagggtctgg cgatcgcccc cttgctcgac
    13141 cgccagcatc agccagcgtt cggtggccat gaagggcatc tgctcgcgca cgtgccgggc
    13201 gatcaccgcg tcgcggacct cgagccccgc ggcgatgttg gtcgcgagga cgaggatggc
    13261 gtcggtggcc aggaacgcct cgggcagcgt gaggcgacgg ttggcgctgt cgtcgagggt
    13321 gcgctcgagc cactgggcgg ccgcggtgtg ggcgccgttg gcctgcagcg agatcatgaa
    13381 gcgcgccagc gcgccgatcc gctcggcgcg catcgggttc cgcttgtagg ccatggcgct
    13441 cgagccgatc tgctccgact cgaacggctc gagcagctcc ccctcgtgct gcagcagccg
    13501 gaggtcgtgc gccatcttgg ccgccgactg cgccacgccg ctcagggcgt cgagcacctg
    13561 gctgtcggcc ttgcgggggt aggtctggcc tgtgacggcg aactgctccg caaagccgag
    13621 cttggcggtg acccgccgct cgagctcgcg caccttggcg tgatcgccgt ggaagagctc
    13681 caggaagctc gcctgggtgc cggtggtccc ctggcagccg cggaagcgga gcgacgcgat
    13741 ccggcggccg aggtcctcgg cgtcgatcgc gaagtcctgc atccagagcg tgatccgctt
    13801 gcccacggtg gtgagctggg ccggctggaa atgggtgtac gcgaggcagg gcagggcgcg
    13861 cgtctgccgg gccttcacct cgagcgccgc gaggagggcc agcacccgcc cgaggatgag
    13921 ctgcatcccc tcgcggagga cgatcaggtc ggcgttgtcg gtgacgaacg cgctcgtggc
    13981 gccaaggtgg agccagggcc gcgcggccgg ggcctggtcg ccgaactggt gcacatgcgc
    14041 catcacatcg tgccggaagc ggcgctcgta ccgcgccgcc gccgccaggt cggcgtcgtc
    14101 gagatgtgcc cgcatctcgg cgagggcccc ggccgggacc gggatcccga gctccgactc
    14161 c
//
LOCUS       DASUAP010000244         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_129721, whole genome shotgun sequence.
ACCESSION   DASUAP010000244 DASUAP010000000
VERSION     DASUAP010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129721"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFU45_06350"
     CDS             <1..184
                     /locus_tag="VFU45_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358718.1"
                     /translation="LVPPPATAAVLLELLDRRGALAPVADIGRWEPAYGRPAEAQRKW
                     EEAHGRPLPDPAGDAG"
     gene            144..602
                     /gene="rimI"
                     /locus_tag="VFU45_06355"
     CDS             144..602
                     /gene="rimI"
                     /locus_tag="VFU45_06355"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU5358719.1"
                     /translation="MDAPCRTRPATPADAPALARLERAVFTDPWSEASLREVLASSGA
                     FGFVAERDGELAGYIFARVVSDEGEILNLAVAPSLRRRGIARALLELALGQFATRRVA
                     QVFLEVRESNVAARELYRRHGFESAGRRAGYYHRPEEAALVLRRVQGSSA"
     gene            708..1193
                     /locus_tag="VFU45_06360"
     CDS             708..1193
                     /locus_tag="VFU45_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU5358720.1"
                     /translation="MSRSLNKATLIGNLGADPEVRTTSNGSKVATLSLATGRQWKNQS
                     GEKQEKTEWHRVVLWNTKFSTLADVAEKYCKKGDKVYVEGSIEYRSWQDREGQTRYTT
                     EINARELILLSGRSGGGDGGESFAPAKAAAAAPAKGKDKDGSFEDFPEALDGEDDDLP
                     F"
     gene            1177..>2047
                     /locus_tag="VFU45_06365"
     CDS             1177..>2047
                     /locus_tag="VFU45_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5358721.1"
                     /translation="MISRSKSLALMLGLAAAAVPAAAVAQNPTLAPAEADSSHTVKSG
                     DTLWGLAQQYLGDPLLWPAIYRLNTSVVEDPHWIYPGEVLRLRAGAAVASVPADTAPA
                     AVAVAAPDTAHAAVVAPAVTPPADTAAAAPAKADTSAAVPAVPADTSAAVAEAAPAAQ
                     DTTPAEPAEQDDTTPIFPPTSGLKQPNILQTTFEDNYRPLRRSDFYSSGFLSERQPLP
                     YGRVAGNVAPLQIHSTGYGSAAAIYSRIALTPPKGGSYQVGDTLLLATVDRTDPTWGD
                     VVEPVGLAKVIDVS"
BASE COUNT          309 a          716 c          733 g          289 t
ORIGIN      
        1 tctcgtgccg ccgccggcca ccgccgccgt cctgctcgag ctgctcgacc ggcgcggcgc
       61 gctggcgccg gtggcggata tcggccgttg ggagcccgcc tacgggcgcc cggccgaggc
      121 ccagcgcaaa tgggaggagg cgcatggacg ccccctgccg gacccggccg gcgacgccgg
      181 ctgacgcacc cgcgctcgcg cgtctcgagc gcgcggtgtt caccgatccg tggtccgagg
      241 cgagcctccg tgaggtgctg gcgtcgagcg gcgccttcgg attcgtcgcg gagcgcgacg
      301 gcgagctggc cggttacatc ttcgcgcggg tcgtgtccga tgagggagag atcctgaacc
      361 tcgcggtcgc accatcgctc cggcgccggg ggatcgcgcg ggcattgctg gagctggccc
      421 tgggccagtt cgcgacccgc cgggtggcac aggtgttcct ggaagtgcgg gaatcgaacg
      481 tcgcggcgcg ggagctctac cgtcggcacg ggttcgaatc ggcaggtcgc cgggccggct
      541 attatcaccg gccggaggag gcggcgctgg tcctccggcg ggtccagggc agctccgcgt
      601 agatcggtac caagtggcgt atgtttggtt gacaaaagga cttgcgattc ctatactgtc
      661 gttgagtccg ctcgtccgtg gggctcggaa tagcactgga gcgtcgtatg agccgcagtc
      721 tcaacaaggc caccctgatc ggcaacctgg gcgctgaccc cgaggtgcgc accacctcca
      781 acggctccaa ggtggccacg ctctccctgg caacgggacg gcagtggaag aaccagtcgg
      841 gggagaagca ggaaaagacg gagtggcatc gggtggtgct gtggaacacg aagttctcca
      901 ccctcgccga cgtcgccgag aagtactgca agaagggcga caaggtgtac gtcgagggca
      961 gcatcgagta ccgctcgtgg caggatcgcg aggggcagac ccggtacacg accgagatca
     1021 atgcccgcga actcatcctc ctctccggcc gctccggcgg cggcgacggc ggtgagtcgt
     1081 tcgccccggc caaggcggcc gcggcggcgc ccgcgaaggg caaggacaag gatggttcgt
     1141 tcgaggactt cccggaggcg ctcgatggcg aggacgatga tctcccgttc taagtcgctc
     1201 gcgctgatgc tgggtctggc agccgccgcg gtccccgcgg cggctgtcgc gcaaaacccg
     1261 acattggcgc ccgccgaggc cgacagctcg cacacggtca agtctggcga cacgctctgg
     1321 ggcctggcgc agcaatacct gggcgacccg ctgctgtggc cggccatcta ccggctcaac
     1381 accagcgtcg tcgaggatcc gcactggatc taccccggcg aggtgctgcg cctgcgcgcg
     1441 ggcgccgcgg tggcgtcagt ccctgccgac acggccccgg cagcggtcgc ggtcgcggcg
     1501 cccgacacgg cgcacgcggc ggtggtcgct ccggcggtga ccccgccggc cgatacggcg
     1561 gccgcggctc cggccaaggc ggatacgagc gctgcggtcc cggctgtgcc ggccgacacc
     1621 agcgcggccg tggccgaggc cgctccggcg gcgcaggata ccacgcccgc ggaaccggcg
     1681 gagcaggacg acaccactcc catcttcccg cccacctccg ggctcaagca gccgaacatc
     1741 ctgcagacca cgttcgagga caactaccgc ccgctcaggc ggtcagactt ctactcgtcg
     1801 ggcttcctga gcgagcggca gccgctgccc tacgggcgcg tcgccggcaa cgtggcgccg
     1861 ctccagatcc actccaccgg gtacggctcc gcggcggcga tctacagccg catcgcgctc
     1921 acgccgccga agggcggctc gtaccaggtc ggggacaccc tcctcctcgc gaccgtcgat
     1981 cgcaccgacc cgacgtgggg cgatgtggtc gagccggtgg gtctcgcgaa ggtcatcgac
     2041 gtgagcc
//
LOCUS       DASUAP010000245         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_129764, whole genome shotgun sequence.
ACCESSION   DASUAP010000245 DASUAP010000000
VERSION     DASUAP010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129764"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFU45_06370"
     CDS             <1..311
                     /locus_tag="VFU45_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5358722.1"
                     /translation="RLALRDHPTDTRILQNMAIAFTKTDRPEEALRHYRRALDVDPSL
                     TGAHYGIGFLLLKRGDAAGAAAHLREFLARPPRGADAERWVRHAESTLRELDAAPGGP
                     "
     gene            308..1006
                     /locus_tag="VFU45_06375"
     CDS             308..1006
                     /locus_tag="VFU45_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358723.1"
                     /translation="MIGRRAFVVLAAAAAMAAACSHLSSGTSPVAIQLVLHQPYELEV
                     GDTVQLNAYALDEAGDSVGAPIIWRAPDTTVTVDSLTGRATAAYPSSTGRIQARTGSL
                     VSDVVTFSTYARSDTIVIDSTADTTTMQPTDSVSAALVASLQSFALGGGSFNRRLSYT
                     ISLPADSSVTLEGGHLAVTVSTLADGTPAAPVHLHKTAAPAPDSAVVVVSAARPSGTV
                     VPGSGQRFVVHILH"
     gene            1019..2845
                     /locus_tag="VFU45_06380"
     CDS             1019..2845
                     /locus_tag="VFU45_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU5358724.1"
                     /translation="MPAPRTLNDIFFGAIDRFTTRPAAMRHKQGGAWRSISYVDLYAR
                     VRDLSFGLATLGIQRGDRVAILSENRPDWAVTDYACLALGAADVAIYPTLPAKQIEYI
                     LNDSGAVAVCVSTAAQLAKIREIRAALPALKHVIGFDAETRGTGVVTLDELLERGRAS
                     GADEAAWRRHALTAQPDEVATFIYTSGTTGDPKGVMLTHGNITSNVVAGLEVLGLRDD
                     DECLSFLPLSHIFERMAGHYVMMQAGVVINYAENFDSVPANLLEIRPTVVLSVPRLYE
                     KIYARVLDNAMGGGPVKRNIFFWAKRTGEECIDLLQARKPIPGALRFGWAIADRLVFT
                     KLRARTGGRLRFFVSGGAPLGADIARFFHAARLPIYEGYGLTETSPVICVNTVEHLKI
                     GTVGRPLPGVEVSIAPDGEILTRGPHVMKGYYHKPDATREAIDAQGWFHTGDVGDLDL
                     EGYLRITDRKKDLIVTAGGKNIAPQPIENAVKASKFMSNAVMLGDKRKFPIMLVVPNF
                     GNLQAWARHKQLAFADDAALIALPEVRSKLEREALAMMRDLAHYEVPKKFLPLLHDFS
                     IENGELTPTLKVKRRVVELHYQKEIDALYAEPEGGIRDLDGK"
BASE COUNT          483 a         1028 c         1024 g          415 t
ORIGIN      
        1 accgcctggc gctgcgggac caccccaccg acacgcggat ccttcagaac atggccatcg
       61 cgttcaccaa gaccgaccgg ccggaggagg cgctgcgcca ttaccggcgg gcgctggacg
      121 tcgacccgtc gctcaccggc gcgcactacg gcatcgggtt cctgctgctc aagcggggcg
      181 atgcggcggg cgccgcggcg cacctgcggg agttcctcgc gcgccccccg cggggagcgg
      241 acgccgagcg gtgggtgcgg cacgccgagt cgacgctgcg cgagctcgac gcggcgccgg
      301 ggggcccgtg atcgggcgtc gggcattcgt tgtgctggcg gcggccgccg ccatggccgc
      361 cgcctgcagc cacctgagct ccgggaccag ccccgtggcc atccagctcg tgctccacca
      421 gccatacgag ctcgaggtgg gcgacaccgt gcagctcaac gcgtacgcgc tcgacgaggc
      481 cggcgacagc gtgggcgctc ccatcatctg gcgggcgcct gacacgaccg tgacggtcga
      541 ctccctgacc ggccgggcca ccgcggcata tcccagttcg accgggcgga tccaggcgcg
      601 gaccggctcg ctggtgagcg atgtggtgac cttcagcacc tacgcccggt cggacaccat
      661 cgtcatcgat tccacggccg acaccaccac gatgcagccg accgactcgg tgtctgcggc
      721 gctggtggcg agcctccagt ccttcgcgct gggcggcggc agcttcaacc ggcggctgag
      781 ctacaccatc tcgctccccg ccgactcgag cgtgacgctg gagggggggc atctggcggt
      841 cacggtgagc acgctcgccg acggcacgcc cgccgcgccg gtgcacctgc acaagaccgc
      901 cgcgccggcg ccggacagcg cggtcgtggt ggtcagcgcc gcccggccga gcggcaccgt
      961 ggtgccgggg tcggggcagc ggttcgtcgt gcacatcctg cactagcctg gaggcgccgt
     1021 gcctgctccg cggaccttga acgacatctt cttcggcgcg atcgaccggt tcaccacccg
     1081 gcccgcagcg atgcgccaca agcagggcgg cgcctggcgg tcgatcagct acgtcgacct
     1141 gtacgcccgg gtgcgggacc tgagcttcgg gctcgcgacc ctgggcatcc agcggggcga
     1201 ccgggtggcc atcctctccg agaaccggcc cgactgggcc gtgaccgact acgcctgcct
     1261 ggcgctcggc gccgccgacg tggccatcta cccgacgctc cccgccaagc agatcgagta
     1321 catcctcaat gactccgggg cggtcgcggt ctgcgtgtcg accgcggccc agctggccaa
     1381 gatccgcgag atccgcgccg ccctgccggc gctcaagcac gtgatcgggt tcgacgccga
     1441 gacccgcggg accggcgtgg tgaccctcga cgagctcctc gagcgcgggc gcgcgtcggg
     1501 cgccgacgag gcggcgtggc ggcgccatgc gctcaccgcc cagcccgatg aagtggccac
     1561 attcatctac acctcaggca ccaccggtga ccccaagggc gtgatgctga cccacggcaa
     1621 catcacgtcg aacgtggtgg cggggctcga ggtgctgggc ctccgggacg acgacgagtg
     1681 cctgtcgttc ctgcccctga gccacatctt cgagcggatg gccggccatt acgtgatgat
     1741 gcaggcgggc gtggtgatca actacgccga gaacttcgac agcgtgccgg ccaacctgct
     1801 cgagatcagg ccgacggtgg tgctgtcggt gccgcgactc tacgagaaga tctacgcgcg
     1861 ggtgctcgac aacgcgatgg gcggcgggcc ggtcaagcgc aacatcttct tctgggccaa
     1921 gcgcaccggc gaggagtgca tcgatctcct tcaggcgcgc aagccgattc ccggcgcgct
     1981 ccggttcggc tgggccatcg ccgaccggct ggtgttcacc aagctccgcg cgcggacggg
     2041 cgggcggctc cggttcttcg tgtcgggcgg cgcgccgctc ggggccgaca tcgcccggtt
     2101 cttccacgcg gcccggctcc cgatctacga gggctacggg ctcaccgaga ccagtcccgt
     2161 catctgcgtc aacaccgtcg agcacttgaa gatcggcacc gtgggccgtc cgctcccggg
     2221 ggtcgaggtc tccatcgccc ccgacggcga gatcctcacc cgcggcccgc acgtgatgaa
     2281 gggctactac cacaagccgg acgccacccg cgaggcgatc gacgcgcaag ggtggttcca
     2341 caccggcgac gtgggggacc tcgacctcga gggttacctc cggatcaccg accggaagaa
     2401 ggacctcatc gtcaccgccg gcgggaagaa tatcgcgccc cagccgatcg agaatgcggt
     2461 caaggccagc aagttcatgt ccaacgcggt gatgctgggc gacaagcgga agttcccgat
     2521 catgctggtg gtgcccaact tcgggaacct gcaggcgtgg gcccggcaca agcagctggc
     2581 cttcgccgac gacgcggcgc tgatcgccct gcccgaggtc cgcagcaagc tcgagcgcga
     2641 ggcgctcgcc atgatgcgcg acctggccca ctacgaggtg ccgaagaagt tcctccctct
     2701 gctccacgac ttcagcatcg agaacggcga gctgacgccg acgctcaagg tgaagcggcg
     2761 ggtggtggaa ctgcactacc agaaggagat cgacgcacta tacgcggagc cggaaggcgg
     2821 gatccgcgac ctcgacggga agtagtcagt aagaagtgag aagtaagaag taagaagtaa
     2881 gaagggagaa gggagaaggg agaagtaaga cggtttgata ggggcgggcg acgatgagtt
     2941 cgcgatgctt
//
LOCUS       DASUAP010000246         5875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_347812, whole genome shotgun sequence.
ACCESSION   DASUAP010000246 DASUAP010000000
VERSION     DASUAP010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347812"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VFU45_06385"
     CDS             <1..750
                     /locus_tag="VFU45_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358725.1"
                     /translation="AARPALDGVDCRVMPGELVAVVGPNGSGKTSLTRALLGVVRPTS
                     GEVRLDGVPIGGWDRRAIARAVGVVLQREETPFPIRVSEAVMMGRYAHIGPVEAPRAV
                     DRAAVRQALERCDVWSLRDRSVDTLSGGEWQRVRMARALAQEPGLLILDEPSAALDLR
                     HEMELFELIGDLVSGGIGALVVSHHLNVAARFASSLLLLSEGRVAAQGPAAEVLRPDV
                     LAAVFGWPVSVAIAPDGRPQVLPLRRSGGAA"
     gene            747..2783
                     /locus_tag="VFU45_06390"
     CDS             747..2783
                     /locus_tag="VFU45_06390"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5358726.1"
                     /translation="MKFRALFAPAALLGCLAWPLAAQRPASLAGEVVAAADGAPLVDA
                     EIQVADLVVRTNDRGAFRLAAVPFGRHVVVVRRIGFRPDTSAVEITPGLERRIVVQLR
                     GAVVEVAPLTISGVPAGASVLNGADLAARGTDLGAALDGWAGISVRRTDGATAVPQLR
                     GSAPEEVLVVLDGITLNDPLTGRADLARIPVAEVARVVVLPGAETARTGGRAMAGVIE
                     ISTRPRTAPSVTTAGGSFGAWSARVSGAVAGIDVSGGVQRLADDYPSADRSGAPTLRS
                     NAGGQTWDALLRTRGTVDATLRVSGADRGLPGNITNPSATGRATDRSVVASLRHDGGF
                     SWSLTGDAVSTRSWDPSAPPRGTGYDDTTTSAGGSGTLGLTWPARLGGWSGDLASGID
                     GRYDAFAGNGVSAGAEFGRVGAHVGADLGAGPWSLSPVLRVDGWSGWGGVAVTGRVDA
                     EWRRGQTALSVGVGNGVTPPVPFDLLFREGVGVALNPGLRPERVTIELTADLRQGGRL
                     AGIPGTLSLRGYAGQVRDLVVWAAGPNFVWSPQNVDVVRRGGELELELRPGQAWTLGA
                     GAALSFINFAAAPHFPVAYRPRDTEHASVAWRGGPWRMDVEWRRLGRRPQYNNGDWNL
                     PAIGLLDAGVERRLWHDLLARLDVRDLADRRPEYVAGMPLPGRSLLFTLTYGAP"
     gene            2780..3817
                     /locus_tag="VFU45_06395"
     CDS             2780..3817
                     /locus_tag="VFU45_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358727.1"
                     /translation="MKRTDLRVLAAALVLTVACADTTAPLPAATEVLLAVNSTANTLS
                     VIPVTAPGTGVAIPLGGISSTPIGVDARNGIAIVPLGFDNSAAIVDLATQAVTHVALP
                     ANSGATGVAIVDDSIAYVANPNLNSVTRINYITRDTATVKVGVYPQAITFTRGKVFVL
                     NGNLDSTYAPAGASWISVVDPVTNAVTDSIGLTGPGNAQFATVGSDGLLYVVQTGSFG
                     SGSGRLSIVDPVQRTELATFSGFGNAPGPVAASGNTLFVASYSEGLMTFATDTRTVTR
                     GAGSGIAIASNASVASDGNGLTYAISSGPCSGGKPGWAHVIRADLVVGDSVSLGECSV
                     GALISAIPPAP"
     gene            3814..4575
                     /gene="recO"
                     /locus_tag="VFU45_06400"
     CDS             3814..4575
                     /gene="recO"
                     /locus_tag="VFU45_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU5358728.1"
                     /translation="MSGAIATQAIVLATVRYSETSKIVRLATRDLGVQSAIAKGALRP
                     RSRFGAALQLLSEGQAHLLVKEGRDLHTLTAFDVQHVRAGLAADLDRFAAANALAEVM
                     LRCAPPAPHPEAYDLLRNGLDALELAPPVVLGVLPIRILWGLVGALGFAPALDACARD
                     GAALGPGKVGFSFREGGVLCPACAPGRSATELAASDLDELRQLAGAGTELPALDERHA
                     AAHRRLAARWIREHLGEGAPLAALDFWLERRWVRP"
     gene            4572..>5875
                     /gene="selB"
                     /locus_tag="VFU45_06405"
     CDS             4572..>5875
                     /gene="selB"
                     /locus_tag="VFU45_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEU5358729.1"
                     /translation="MIIGTAGHIDHGKSALVEALTGRSVDRLAEERRRGITIDLNFAP
                     LALADGTVAGVIDVPGHEDLVRTMIAGASGMHLVLLVVAADAGIQPQTLEHLAVVESL
                     GVRDGIAVLTKSDLVDRSTLEVRLGELRERLGASPVRFGEAAVVSARTGEGIAALRER
                     LAACRRPAAAPDAFRLPIDRCFSVAGVGTVVTGTAWSGTLAVGDAVRLLPSGAEGRVR
                     SIEMYGASANASIPGARVALGLAGVDRDAIGRGEWVVTADLPWRPTSALDVAVDLQPA
                     ASTPLASRTRVRVHLGTAEVMARVVTATPVEPGGSGEVRLTLESPVLARGGDRVVLRA
                     FSPVGTLGGGRVLDPAPPSRSRAADLRGAVAGDQLLAALVRRRLDGLPLAEVPIVGGV
                     APVAVERAAKGLAGIRRVGGHLVAGDRLLGLQRSLGVAVEAR"
BASE COUNT          695 a         2085 c         2230 g          865 t
ORIGIN      
        1 gcggcgcgtc ccgcgctcga cggggtggac tgccgtgtca tgccggggga actcgtggcg
       61 gtggtcggcc ccaacggcag cgggaagacc tcgctcacgc gcgccctgct cggggtggtg
      121 cgccccacga gcggcgaggt ccggctcgac ggcgtgccga tcggcggatg ggaccggcgc
      181 gcgatcgcgc gggcggtcgg tgtggtgctc cagcgggagg agaccccgtt cccgatccgg
      241 gtgtccgagg cggtgatgat ggggcggtat gcgcatatcg gcccggtgga agcgccacgc
      301 gccgtggacc gggcggcagt ccgccaggcc ctcgagcgct gcgacgtctg gtcgctccgg
      361 gaccggtcgg tcgacaccct ctccggaggg gaatggcaac gggtgcggat ggcgcgggcg
      421 ctggcgcagg agcccggact cctgatcctc gacgagccga gcgccgcgct cgatctccgc
      481 cacgagatgg aactgttcga gctgatcggc gacctggtgt cgggcggcat cggcgcattg
      541 gtggtgagcc atcacctcaa cgttgcggcg cggttcgcat cgtcgctcct gctgctgagc
      601 gaggggcgcg tcgccgccca gggcccggca gcggaggtcc tccggcccga cgtgctggcc
      661 gcggtgttcg gctggccggt cagcgtggcg atcgcgccgg acgggcgtcc gcaggtgctg
      721 ccccttcgcc gcagcggagg cgccgcgtga agttccgcgc gctcttcgcc ccggcggcgc
      781 tcctcgggtg cctcgcgtgg ccgctggcgg cccagcgacc cgcgtcgctc gcaggcgagg
      841 tggtggcggc ggccgacggt gcgccgctcg tcgatgccga gatccaggtg gccgacctcg
      901 tggtccgcac caacgaccgt ggtgcgttcc gcctggcggc ggtcccgttc gggcgccacg
      961 tggtggtggt ccggcgcatc ggcttccgtc cggacacctc cgcggtcgag atcactccgg
     1021 gcctcgagcg ccggatcgtg gtgcagctgc ggggtgccgt ggtcgaagtg gcgccgctca
     1081 ccatcagcgg cgtcccggcg ggggcgtcgg tgctgaacgg cgccgacctc gccgcgcggg
     1141 ggacggacct tggcgcggca ctcgacgggt gggccgggat ctcggtccgg cgcaccgacg
     1201 gggccaccgc ggtgccccag ctccgcggga gcgcgcccga ggaggtgctc gtcgtgctgg
     1261 acgggatcac gctcaacgac cccctgaccg ggcgggccga cctggcccgg atccccgtcg
     1321 ccgaagtggc gcgggtcgtg gtcctgccgg gagccgagac ggcccggacc gggggcaggg
     1381 cgatggccgg cgtgatcgag atctcgaccc gaccccgcac ggcaccatcg gtgacgaccg
     1441 ccggcggcag tttcggtgca tggagcgcgc gcgtgagcgg tgcggtggcg gggatcgacg
     1501 tgtcgggagg ggtgcagcgc ctggcggatg actacccgtc cgctgatcga tcgggggcgc
     1561 cgacgctccg gtcgaatgcc ggcggccaga catgggacgc gctcctgcgg acccggggca
     1621 ccgtggacgc gacgctccgg gtatcgggcg ccgatcgcgg gctgcccggc aatatcacga
     1681 acccgtccgc gaccggtcgg gccaccgacc ggtcggtggt ggcatcgctc cggcacgacg
     1741 gcgggttctc ctggtccctg accggcgacg ccgtttccac ccgctcgtgg gatccatcgg
     1801 cgccgccgcg cgggaccggg tatgacgaca ctacgacgag cgccggcggg agcggcacgc
     1861 tgggcctgac ctggccggcc cggctcggcg gatggtccgg cgacctggca tccggcatcg
     1921 atgggcgata tgacgcgttc gccgggaacg gtgtcagtgc gggcgcggag ttcggccgcg
     1981 tcggggccca cgtcggggcg gacctcggtg cgggaccatg gagcctctct ccggtgcttc
     2041 gggtcgacgg ctggtcgggg tggggtgggg tggcggtcac gggaagggtc gatgcggagt
     2101 ggcggcgcgg gcagacggca ttgtccgtcg gcgtcggcaa cggcgtcacg ccgccggtgc
     2161 cgttcgacct gctctttcgg gaaggggtgg gcgtcgcgct caatccgggt cttcgtccgg
     2221 agcgggtcac gatcgagctc acggccgacc tccgccaggg gggccggctc gcggggatac
     2281 cggggacgct ctcgctccgc gggtatgccg gccaggtccg cgacctggtc gtgtgggcgg
     2341 cgggacccaa cttcgtctgg agcccccaga acgtcgacgt cgtgcgccgc ggcggcgagc
     2401 tcgagctgga gcttcgccct ggtcaggcct ggaccctggg cgcgggggcg gcgctcagtt
     2461 tcatcaactt cgctgcggca ccacatttcc cggtggccta ccgcccccgc gacacggagc
     2521 acgcttccgt cgcgtggcgc ggcgggccct ggcggatgga cgtcgaatgg cgacggctgg
     2581 gccggcggcc gcagtacaac aatggggact ggaaccttcc ggccatcggc ctcctcgacg
     2641 ccggcgtcga gcggcggctc tggcacgacc tcctggcccg gctcgacgtc cgggacctgg
     2701 ccgaccgacg cccggaatat gtggcgggga tgccgctccc cgggcgcagc ctgctcttca
     2761 ctctcacgta tggtgcacca tgaagcggac cgacctgcgg gtgctcgccg ccgccctcgt
     2821 tctcactgtc gcgtgcgcgg acaccaccgc gccccttccg gctgccaccg aggtgctgct
     2881 cgccgtcaac agcaccgcca ataccctcag cgtcataccg gtcacagcgc ccggcaccgg
     2941 cgtcgccatt ccgctgggtg ggatcagttc cacgccgatc ggcgtcgacg cccggaacgg
     3001 catcgccatc gttccgctcg gctttgacaa ctcggccgcc atcgtggacc tcgcgacgca
     3061 ggccgtcacg cacgtggcgc tccccgccaa ttccggcgcc accggggtcg ccatcgtcga
     3121 cgactcgatc gcctatgtgg ccaacccgaa cctcaattcg gtcacccgga tcaattacat
     3181 cacccgggac accgccaccg tgaaggtcgg cgtgtatccg caggccatca ccttcacccg
     3241 cggcaaggtg ttcgtgctga acgggaacct cgactcgacg tacgcgcccg cgggagcaag
     3301 ctggatctcg gtcgtggacc cggtcaccaa tgcggtcacg gactccatcg ggctcacggg
     3361 cccggggaac gcccagttcg cgacggtcgg ctccgacggc ctcctgtacg tcgtccagac
     3421 gggctccttc ggttcgggga gcggtcggct ctcgatcgtc gacccggtgc agcggaccga
     3481 actggcgacc ttttccgggt tcggcaacgc accgggaccg gtggcggcga gcgggaacac
     3541 actcttcgtc gcctcgtatt ccgagggcct gatgaccttc gccaccgaca cccgcacggt
     3601 gacgcgcggc gccggctcgg ggatcgcgat cgcatccaac gcgagtgtgg ccagcgacgg
     3661 caacgggctc acctatgcga tcagctcggg cccctgttcc ggtggcaagc ccgggtgggc
     3721 ccacgtcatc cgcgcggacc tggtggtcgg ggactcggtg agtctcggcg aatgcagcgt
     3781 gggcgccctc atctcagcga tcccaccggc cccgtgagcg gagcgatcgc gacccaggcg
     3841 atcgtgctcg ccacggtccg ctacagcgag acctccaaga tcgtccggct cgcgacccgc
     3901 gaccttggcg tgcagagcgc catcgcgaag ggtgcgctgc gtccccgctc gcggttcggc
     3961 gctgcgctcc agttgctgag cgaggggcag gcgcacctcc tggtcaagga agggcgagac
     4021 ctccacaccc tcacggcgtt cgacgtgcag cacgtccgcg ccggcctggc cgcggacctc
     4081 gaccggttcg ccgccgccaa cgccctggcc gaggtgatgc tgcgctgtgc cccgccggcg
     4141 ccccatcccg aggcctacga cctcctgcgc aacgggctcg acgcgctcga acttgccccg
     4201 cccgtggtcc tcggcgtcct gccgatccgg atcctctggg gactggtggg cgccctgggc
     4261 tttgccccgg cgctcgatgc ctgcgcccgc gacggggcgg cgctcggccc cgggaaggtc
     4321 gggttcagct tccgcgaggg cggtgtgctg tgccccgcgt gcgcgccggg ccgctcggcc
     4381 acggagctgg cggcatcgga cctggacgag ctgcgccaac tggcgggtgc gggaacggag
     4441 cttccggcgc tcgacgagcg gcacgcggcg gcacaccggc gactggcggc gcggtggatc
     4501 cgcgagcacc tgggcgaggg ggcgccgctc gccgccctcg acttctggct tgagcgccgc
     4561 tgggtccgcc cgtgatcatc ggcaccgccg gtcatatcga ccacggcaag tcggcgctgg
     4621 tggaggccct gacgggacgg tcggtggatc gcctggccga ggagcggcgg cgcggcatca
     4681 ccatcgacct caacttcgcg ccgctggcgc tcgctgacgg aacggtcgcc ggcgtgatcg
     4741 acgtaccggg ccacgaagac ctggtccgga cgatgatcgc cggcgcatcc gggatgcacc
     4801 tcgtcctcct ggtcgtcgcg gccgatgccg ggatccagcc ccagacgctg gagcacctgg
     4861 ccgtggtcga gtcactcggc gtccgggacg ggatcgcggt gctcaccaag tcggacctgg
     4921 tcgaccggtc cacgctcgag gtgcgcctcg gtgaactgcg cgagcgcctt ggcgcgtcgc
     4981 cggtgcggtt cggcgaggcg gcggtcgtct ccgcccggac cggcgagggg atcgccgcgc
     5041 tgcgggagcg tctcgccgcg tgtcggcgcc cagccgcggc tcccgatgcg ttccgcttgc
     5101 cgatcgaccg ctgcttctcc gtggcaggag tgggcaccgt cgtcactggc acggcatggt
     5161 ccgggacctt ggcggtcggg gacgccgtgc ggctcctgcc ctccggcgcc gaaggccgcg
     5221 tgcgttcgat cgagatgtac ggtgcgtccg cgaatgccag tatcccgggg gcgcgcgtcg
     5281 ccctgggcct cgccggcgtc gaccgggatg cgatcggccg tggggaatgg gtcgtcaccg
     5341 ccgatcttcc ctggcgcccg acctcggcgc tcgatgtcgc cgtcgacctg cagccggcgg
     5401 cgtccacgcc gctcgcatcg cgcacgcggg tccgggtcca ccttgggacc gcggaggtga
     5461 tggcgcgggt cgtcacggcg acgccggtcg agccgggtgg gagcggggaa gtgcgcctga
     5521 cgctcgagtc accggtactg gcgcgtggcg gcgaccgggt cgtgttgcgg gcgttcagcc
     5581 cggtgggcac gctgggcgga ggccgcgtgc tcgatcccgc gccgccatcg cggagccggg
     5641 ccgcggacct tcgcggggcc gtcgcgggcg accagctcct cgcggcgctc gtccggcggc
     5701 ggctcgacgg gttgccgctc gcggaggtgc cgatcgtcgg cggtgtcgcg ccggtggcgg
     5761 tcgaacgtgc ggccaagggg ctcgccggca tccgccgtgt gggcggtcac ctggtggccg
     5821 gggaccggct cctcgggctc cagcggagcc tcggcgtcgc ggtcgaggcg cgaca
//
LOCUS       DASUAP010000247         7197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_266336, whole genome shotgun sequence.
ACCESSION   DASUAP010000247 DASUAP010000000
VERSION     DASUAP010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266336"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFU45_06410"
     CDS             complement(<1..529)
                     /locus_tag="VFU45_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU5358730.1"
                     /translation="MSTPLAAYPRHLSRAAGDAGARLIARLERWGMGEPAILTTVAVG
                     VGILAGLSVLVFYRSIDVAAEVIARIADALEIPTSIVAAASLAIGLIAVRLLVHYGTR
                     DSEGENIPDAMHAVARRGGVLNLRPVAIKTLAAAVTLGSGGSLGAEGPVAVLGAAVGS
                     RAGRWLRFRKDRLRLL"
     gene            690..2267
                     /gene="atpA"
                     /locus_tag="VFU45_06415"
     CDS             690..2267
                     /gene="atpA"
                     /locus_tag="VFU45_06415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU5358731.1"
                     /translation="MATEATLRPGELKDVLLKEIEAADLASTDARDVGTVLEVKDGVA
                     RIYGLAAAMAGELLEFTVRETGEAIAGLVLNLEEDNVGAAVMGDYLKLKEGDEVRCTG
                     KVLDVPVGPAMLGRVVDALGRPVDGRGPIKASGTRLVEMIAPGIIVRQPVKEPMQTGI
                     KAIDAMIPIGRGQRELIIGDRGTGKTAIAIDTIINQKGAGVVCVYVAIGQKRSTVASV
                     VEKLRQAGALEYTVVVVASASDPAPLQYIAPYAGCAIAEYFMYEEGKATLCVYDDLSK
                     QAAAYRQLSLILRRPPGREAYPGDVFYLHSRLLERAAKISENAETVKYDPRIKKPGGS
                     LTALPIIETQAGDVSAYIPTNVISITDGQIFLITDLFYSNVRPAVDAGISVSRVGGNA
                     QIKAMKQVAGPLRLSLAQYRELEAFAQFGSDLDPATQRQLARGARLVEILKQPQYQPV
                     PVERQVAIIWVVTNGHLDDVDVKHIRQWEQDFLGYLDSAHPKVLEGIRTKKALDDQLT
                     GQLKAAVDGFKPTFRPA"
     gene            2270..3148
                     /gene="atpG"
                     /locus_tag="VFU45_06420"
     CDS             2270..3148
                     /gene="atpG"
                     /locus_tag="VFU45_06420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU5358732.1"
                     /translation="MAKGKALKGRIKSAQNIRKITRTMELVSTSKLKRAQDRVVGARP
                     YAEALAQVIADLYGPELAERFPLLRRPAPPAKGGPTRAAVILVTSNRGLAGGFNSNLI
                     KEARRRIEDLEGRGYTVELHAVGRKGIGFFKYLGRPMATQRQDIGDKPTAAHAAELVA
                     PLIAAYAAGELASVDLVYAQFKSAISTPPTTLRVLPVAAPALKGARPDYILRPSADGI
                     LGELLPLYVRNAIYRGLVETAAAEHGARRTAMKNATDNAGEIIDLLRRSYNRQRQATI
                     TQEISEIVGGAAALQG"
     gene            3271..4695
                     /gene="atpD"
                     /locus_tag="VFU45_06425"
     CDS             3271..4695
                     /gene="atpD"
                     /locus_tag="VFU45_06425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU5358733.1"
                     /translation="MATATQNIGKVVQVIGPVLDVEFEPGLLPEIYNALLLEDRSGTV
                     PVRLVAEVQQHIGQNQVRAVAMSTTDGVVRGMKVVDTGAAISVPVGNVPLGRILNVLG
                     EPVDGGAAIPATAERWAIHRETPKFTDLEAKTQVFETGIKVIDLIAPFVKGGKIGLFG
                     GAGVGKTVVIQELINNVAKGHGGKSVFCGVGERTREGNDLYLEFQEAGILPNVALIYG
                     QMNEPPGARLRVGLSGLTVAEYFRDIEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSA
                     VGYQPTLATEMGDLQERITSTNKGSITSVQAIYVPADDLTDPAPATAFAHLDAQVVLS
                     RAISELGIYPAVDPLDSSSRILDAQYLGERHYNVAVGVQRILQRYKSLQDIIAILGMD
                     ELSEEDKQVVARARRVQRFLSQPFHVAEQFTGFPGKYVKLEETVESFERVIAGEFDHL
                     PEQAFYMVGGIDEAVEKAKKLASA"
     gene            4695..4952
                     /gene="atpC"
                     /locus_tag="VFU45_06430"
     CDS             4695..4952
                     /gene="atpC"
                     /locus_tag="VFU45_06430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEU5358734.1"
                     /translation="MRVTVISPEAAIFDGDADALTVPAYDGSVGVLPYHAPLMTLLGE
                     GVLTIHRGDVSDRYQVRGGFVQVAHDTVRVVAEHVQGAKHA"
     gene            4945..5550
                     /locus_tag="VFU45_06435"
     CDS             4945..5550
                     /locus_tag="VFU45_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358735.1"
                     /translation="MRKLTVALLLAGAVMPVALSAQSTGTPVFAAPYRAFQRSEAGVS
                     LSDPVGGGVGLEGFYRIGSHAWDIGFRGGFDAPNGNANTALLLGVDGRTRVLTHDEGF
                     PLDGALTLGFGGRFVSGGSELLIPVGLSMGRRLDLEGSNISFVPYVQPVLTPVFASGN
                     SHLDFTLGLGVDMRVSRRVDLRVAGGIGDLDGVSVSVAFIH"
     gene            5654..6301
                     /locus_tag="VFU45_06440"
     CDS             5654..6301
                     /locus_tag="VFU45_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358736.1"
                     /translation="MRSKLVAALAFASVAFAAPAVAQVGGLPVFNAGIGTGIGINVDA
                     GFPDSAAGKGHTYGLTGSVGLGPLGFTAIVASQKPEGASSSTTWAGATANLKLFGGPL
                     VPVTVTGQVGVGGASPSSGTTLLNVPVSIGIAVNIPSPALSIKPWIAPRMQYTKISTP
                     LGSTNSTKFGFSAGVNLGLSMGLGIRVAYDYYKPDTGAQSVFSVGAGYNIHVPGL"
     gene            6366..6803
                     /locus_tag="VFU45_06445"
     CDS             6366..6803
                     /locus_tag="VFU45_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358737.1"
                     /translation="MTLRLPRPLRAALPLALLAVGACAQTFDATTLGVPATLSSDASA
                     PAQGVHFQVRATQVWFGWGLLSINEPTLKDALAAQLIGGKSVADVKIRSTTRFTNLLV
                     TVLTGGLLVPRTVEFDGTIVGDAGAPATPSTPAQAPPQAPAKP"
     gene            complement(6953..>7197)
                     /locus_tag="VFU45_06450"
     CDS             complement(6953..>7197)
                     /locus_tag="VFU45_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358738.1"
                     /translation="IFGPGWQDSRDAAALIEAGGADALPALLPGDALARTWERWIGDD
                     AARAEAGRRARGVIDRGLGAAGRTAQMVEGLVAGKT"
BASE COUNT         1131 a         2591 c         2450 g         1025 t
ORIGIN      
        1 ggaggagcct gaggcgatcc ttgcggaagc ggagccagcg gccggcgcgg ctcccgaccg
       61 cggcaccgag caccgcgacc gggccttcgg cgccgaggga gccgccgctc ccgagggtga
      121 ccgcggcggc aagggtcttg atggcgacgg ggcggaggtt gaggacgccg ccacgccggg
      181 cgacggcgtg catcgcgtcg gggatattct cgccctcgga gtcgcgggtg ccgtagtgca
      241 ccaggagccg cacggcgatg agcccgatgg cgagggacgc ggcggccaca atggatgtcg
      301 ggatctccag cgcgtccgcg atccgcgcga ttacctcggc agcgacgtcg atcgaacggt
      361 agaagacaag gaccgagagg ccggccagga tgcccacgcc caccgcgacg gtggtgagga
      421 tcgccggctc gcccatcccc caccgttcga ggcgggcgat gaggcgggcg ccggcatccc
      481 ccgcggcgcg ggaaaggtgg cgggggtagg cggcgagggg ggtcgacatg ccgcaggata
      541 tatagataga ggcatgaggc gtagcccccg gcccggggcc cgcccttgcc gccgccgttc
      601 cgcggggcga aacttgccat tggcgtcgat tgtgataaat ttcacaagct gaagccgaag
      661 tgcaccgatc cctctctggc gtgagaacca tggcgactga agcgaccctg cgacccggcg
      721 aactcaagga cgtcctgctc aaggagatcg aggcggcgga tctggcctcg accgacgcgc
      781 gggacgtggg caccgtcctc gaggtgaagg acggggtggc ccggatctac ggccttgccg
      841 ccgcgatggc gggcgagctc ctcgagttca ccgtgcggga gaccggcgag gcgatcgcgg
      901 ggctcgtgct caacctcgaa gaggacaacg tcggcgccgc cgtgatgggc gactacctga
      961 agctcaagga aggcgacgag gtccgctgca ccggcaaggt gctcgacgtt ccggtgggcc
     1021 cggcgatgct cggccgcgtg gtggacgcgc tgggccgccc ggtcgacggg cgcgggccga
     1081 tcaaggccag cggtacccgg ctggtggaga tgatcgcccc gggcatcatc gtgcggcagc
     1141 cggtcaagga gccgatgcag accggcatca aggccatcga cgcgatgatc ccgatcggcc
     1201 gcggccagcg cgagctcatc atcggcgacc gcggcaccgg caagaccgcc atcgccatcg
     1261 acacgatcat caaccagaag ggcgccgggg tggtgtgcgt gtacgtggcg atcggccaga
     1321 agcgctcgac ggtcgcgtcg gtggtggaga agctccggca ggcgggcgcg ctcgagtaca
     1381 ccgtggtcgt ggtggcctcg gcgtccgacc cggcgccgct gcagtacatc gcgccctatg
     1441 ccgggtgtgc catcgccgag tacttcatgt acgaggaggg gaaggcgacc ctctgcgtct
     1501 atgacgacct ctccaagcag gccgcggcgt accggcagct ctcgctcatc ctgcggcgtc
     1561 cgccggggcg cgaggcctac ccgggcgacg tgttctacct gcacagccgg ctcctggagc
     1621 gcgcggccaa gatctcggag aacgccgaga ccgtgaagta cgacccccgc atcaagaagc
     1681 cgggcgggtc gctcacggcg ctcccgatca tcgagaccca ggcgggcgac gtctcggcct
     1741 acatcccgac caacgtcatc tcgatcaccg acggccagat cttcctgatc accgacctct
     1801 tctactccaa cgtccgcccc gcggtggatg ccggcatcag cgtgagccgc gtcggcggca
     1861 acgcgcagat caaggcgatg aagcaggtgg ccggcccgct ccggctctcg ctggcgcagt
     1921 accgcgagct cgaggcgttc gcccagttcg gctccgacct cgaccccgcc acccagcgcc
     1981 agctcgcccg gggcgcccgg ctggtggaga tcctcaagca gccgcagtac cagccggtgc
     2041 cggtcgagcg ccaggtggcc atcatctggg tcgtcaccaa cggccacctc gacgacgtcg
     2101 acgtgaagca catccgccag tgggagcagg acttcctggg ctacctcgac tccgcgcacc
     2161 cgaaggtgct cgaggggatc cggaccaaga aggcgctcga cgaccagctc accggccagc
     2221 tcaaggcggc ggtcgacggc ttcaagccca ccttccggcc ggcctgacga tggccaaggg
     2281 caaggcgctc aagggccgga tcaagtccgc gcagaacatc cgcaagatca cgcggacgat
     2341 ggagctcgtc tccacgtcca agctgaagcg ggcgcaggac cgcgtggtcg gcgcccggcc
     2401 gtacgccgag gcgctggcgc aggtcatcgc cgacctgtac ggccccgagc tggcggagcg
     2461 gttcccgctg ctccggcgcc cggcgccgcc ggccaagggc gggccgaccc gcgccgcggt
     2521 catcctggtg acgtcgaacc gcggactcgc cggcgggttc aactcgaacc tgatcaagga
     2581 agcgcgccgg cggatcgagg acctcgaagg ccgcggctac accgtcgagc tccacgccgt
     2641 cggccggaag ggcatcggat tcttcaagta cctcggccgc ccgatggcca cgcagcgcca
     2701 ggacatcggt gacaagccga ccgccgccca cgccgccgag ctcgtggccc cgctcatcgc
     2761 ggcgtatgcg gcgggggagc tggcgagcgt cgacctggtc tatgcccagt tcaagagcgc
     2821 catcagcacg ccgccgacga cgctccgggt cctcccggtc gcggccccgg cactgaaggg
     2881 cgcgcggccc gactacatcc tccgcccctc ggcggacggg atcctgggcg agctcctccc
     2941 gctgtacgtc cggaacgcca tctaccgcgg gctggtggag acggcggcgg cggagcacgg
     3001 cgcccggcgg acggcgatga agaacgcgac cgacaacgcg ggggagatca tcgacctcct
     3061 ccgccgcagc tacaaccgcc agcgccaggc gacgatcacg caggagatca gcgagatcgt
     3121 cggcggcgcc gcggcgttgc agggctaggc gcaaaggcgc gtagacggat agacggacgg
     3181 ccggaggaac gtgcgcgccg catcgctgat gcgacctggc gtcctccgcc catccgccgg
     3241 tgcacaacca gacccaagga cccgaacgca atggcaactg caacgcagaa catcggcaag
     3301 gtcgtccagg tcatcggccc ggtgctcgac gtcgaattcg agccggggct cctgcccgag
     3361 atctacaacg ccctcctcct cgaggaccgc tccggcacgg tgccggtgcg cctggtggcg
     3421 gaggtgcagc agcacatcgg ccagaaccag gtccgcgcgg tggcgatgag caccaccgac
     3481 ggcgtggtcc gcgggatgaa ggtggtcgac accggtgccg ccatctcggt gccggtcggg
     3541 aacgtccccc tcgggcggat cctcaacgtc ctgggcgagc cggtggacgg cggggccgcc
     3601 atcccggcca cggcggagcg gtgggccatc caccgcgaga cgcccaagtt caccgacctc
     3661 gaggccaaga cccaggtctt cgagaccggc atcaaggtca tcgacctgat cgccccgttc
     3721 gtgaagggcg gcaagatcgg gctcttcggc ggcgccggcg tcggcaagac ggtcgtcatc
     3781 caggagctca tcaacaacgt cgccaagggt cacggcggca agtcggtgtt ctgcggcgtg
     3841 ggcgagcgca cccgcgaggg gaacgacctc tacctcgagt tccaggaggc ggggatcctc
     3901 cccaacgtgg cgctcatcta cggccagatg aacgagccgc cgggcgcgcg gctccgcgtc
     3961 ggcctgtccg gcctcacggt ggcggagtac ttccgcgaca tcgagggcca ggacgtgctc
     4021 ctgttcatcg acaacatctt ccgcttcacc caggcgggct ccgaggtctc agccctcctc
     4081 ggccggatgc cgagcgcggt cggctaccag ccgacgctcg ccaccgagat gggcgacctg
     4141 caggagcgga tcacctcgac caacaagggc tcgatcacct cggtgcaggc catctacgtg
     4201 ccggccgacg acctgaccga cccggcgccg gccaccgcct ttgcccacct ggatgcgcag
     4261 gtggtgctct cgcgcgccat ctccgagctc ggcatctatc ccgccgtcga cccgctcgac
     4321 agctcgagcc ggatcctcga cgcccagtac ctgggcgagc gccactacaa cgtggccgtc
     4381 ggcgtgcagc ggatcctcca gcgctacaag agcctgcagg acatcatcgc gatcctcggg
     4441 atggacgaac tctccgagga ggacaagcag gtggtggcgc gggcacgccg ggtgcagcgc
     4501 ttcctgtccc agccgttcca cgtggccgag cagttcaccg gcttcccggg caagtacgtc
     4561 aagctcgagg agaccgtgga gagcttcgag cgcgtcatcg ccggcgagtt cgaccacctg
     4621 cccgagcagg cgttctatat ggtgggcggc atcgacgagg cggtcgagaa ggccaagaag
     4681 ctggcctcgg cgtgatgcgc gtcacggtca tctcgcccga ggccgccatc ttcgatggtg
     4741 acgccgacgc cctgaccgtc ccggcctacg acggctcggt cggcgtcctg ccgtaccacg
     4801 ccccgctcat gaccctgctg ggggagggcg tgttgacgat ccatcgcggc gacgtctccg
     4861 accggtacca ggtccgcggc gggttcgtgc aggtcgccca cgacacggtc cgcgtcgtgg
     4921 cggaacacgt gcaaggagcc aagcatgcgt aaactgaccg tggcgctgct gctggccggc
     4981 gccgtgatgc ccgttgccct gtccgcgcag tccaccggca cgccggtgtt cgccgcccca
     5041 taccgcgcgt tccagcgctc ggaagcgggg gtctcgctct ccgacccggt gggcggcggg
     5101 gtgggcctcg aaggattcta ccgtatcggc agccacgcct gggacatcgg cttccgtggc
     5161 gggttcgacg cccccaacgg gaacgccaac acggcgctcc tcctcggcgt cgatggccgg
     5221 acccgggtgc tgacccacga cgaggggttc cccctcgacg gcgccctgac cctcgggttc
     5281 gggggccggt tcgtgagcgg cgggtcggag ctcctgatcc cggtgggcct ctcgatgggg
     5341 cgccggctcg acctcgaagg ttcgaacatc agcttcgtcc cctacgtgca gccggtgctg
     5401 acgcccgtgt tcgcgtcggg caacagccac ctcgacttca ccctcggcct cggggtggac
     5461 atgcgggtgt cgcggcgggt ggacctccgg gtggccggcg gcatcggcga cctcgacggc
     5521 gtgagcgtga gcgtggcgtt catccactga cggcctgaat tgacttcccc ggcgcgtccg
     5581 cgatagcttg cggcgcgccg gttcgcgtcg ggcgcattgg ctcccggcat acaatcaccc
     5641 gtaggaggct cagatgcgct cgaagctcgt cgcggcactc gccttcgcgt cggtcgcctt
     5701 cgccgccccc gcggtcgcgc aggtcggcgg acttccggta ttcaatgcag ggatcggcac
     5761 cgggatcggc atcaatgtcg atgctgggtt cccggattcg gcggccggca aaggccacac
     5821 ctacggcctg accgggtcgg tggggctcgg accgctcgga ttcaccgcca tcgttgcctc
     5881 ccagaagccg gaaggcgcgt cgtccagcac cacgtgggca ggcgcgacag ccaatctcaa
     5941 gctcttcggg ggcccgctcg tcccggtcac ggtcaccggc caggtggggg tcggcggagc
     6001 gtcaccttcg tccgggacga cgttgctcaa tgtgcccgtg agcatcggca tcgccgtcaa
     6061 catcccgagc ccggcgctgt cgatcaagcc gtggatcgcg ccgaggatgc agtacaccaa
     6121 gatctccacc ccgctcggca gcacgaactc caccaagttc ggattcagcg ccggcgtgaa
     6181 ccttggcctg tcgatgggcc tcgggatccg tgtcgcgtac gactattaca agcccgacac
     6241 cggtgcgcag tcggtcttct ccgtcggcgc gggttacaac atccacgttc ccggcctctg
     6301 atccaccccg tggggaggcg cgccgcggtg gcgcgccttc cccgggctcc tcttccggta
     6361 ccctcgtgac ccttcgcctg ccacggcccc tccgggccgc gctcccgctc gcactccttg
     6421 ccgtcggtgc ctgcgcccag accttcgacg ccaccaccct cggcgtgccc gccacgctct
     6481 cctccgacgc gtcggcgccg gcccagggcg tgcacttcca ggtccgcgcg acccaggtct
     6541 ggttcgggtg ggggctcctc tcgatcaacg agccgacgct caaggacgcg ctcgccgccc
     6601 agctgatcgg cgggaagagc gtggccgacg tcaagatccg gagcaccacc cggttcacca
     6661 acctcctggt gaccgtcctc accggcggcc tcttggtccc gcgaacggtg gaattcgacg
     6721 gcaccatcgt cggcgacgcc ggcgcgccag ccacgccgtc cacgcctgcc caggcccctc
     6781 cgcaagcacc cgccaagccc tgaacgccgt gcccggatcc gcgatccggc ccggcgccag
     6841 gtgaacacag caggatgctg gatgcacaac ggggagcgct tcatacgctc cccgttcgtc
     6901 attccacccc gtcattgtta ccacttacct cttaccgctc accgctcacc gctcacgtct
     6961 tacctgcaac tagcccctcc accatctgcg ctgttcgacc ggctgcaccc agcccccggt
     7021 cgatgacccc gcgggctcgc cggccggcct cggcacgcgc ggcgtcgtcc ccgatccacc
     7081 gctcccaggt cctcgccagc gcatccccgg gcaggagggc cgggagcgca tccgcgccgc
     7141 cggcctcgat caatgcggcg gcgtcccggc tgtcctgcca gccggggccg aagatga
//
LOCUS       DASUAP010000248         9644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_470539, whole genome shotgun sequence.
ACCESSION   DASUAP010000248 DASUAP010000000
VERSION     DASUAP010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470539"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFU45_06455"
     CDS             <1..243
                     /locus_tag="VFU45_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358739.1"
                     /translation="FAEGFAGDPGWHVQLMNETVDVARSGDLAVYRGVYHEDHGAPGA
                     LLTHRTNFIAEFRRQHDRSWKIAWYSVIRMEPSHPK"
     gene            332..1240
                     /locus_tag="VFU45_06460"
     CDS             332..1240
                     /locus_tag="VFU45_06460"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEU5358740.1"
                     /translation="MVALPLLAAAAFVASTTLLDRTNGTLISSGVERRYLLFVPPTYD
                     PSRPTPLVISLHGAAAWPAQQMHLTHWNDVAAEHGFIVVYPAARGRIWRVQHPGSDPT
                     ADVRFIGDLIDTLERTYHIDSHRIYANGFSLGGAMAFALGCRLSGRLAAVGTASAAEA
                     LPWSWCTDDRPMPFINFHGTADLVPYAGGPSPDPFNPVTFPAVRQWTAQWAGRNRCRG
                     QGDSTLAPDVVATAYRDCADDASVVLYTVRGGGHAWPGGKALPRWPFGRTTTSIDATR
                     IMWAFFQAHPLGPTTSGRSVPPREEH"
     gene            1279..1695
                     /locus_tag="VFU45_06465"
     CDS             1279..1695
                     /locus_tag="VFU45_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEU5358741.1"
                     /translation="MPLARKLGIREGARILSLGAPDGYAKLLGPLPDDARIVARLSAA
                     VDLVHLFTTRRTELTRELARLRRAIRPDAAVWVSWPKKAARADTDVTEDVIRAVALPL
                     DFVDIKVCAVDERWSGLKLVIRKAARPASPRDGARR"
     gene            1798..2190
                     /locus_tag="VFU45_06470"
     CDS             1798..2190
                     /locus_tag="VFU45_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358742.1"
                     /translation="MPASPVAQRILTLSPAVRYVAIYHRGTLTTATRPDLANASSSES
                     DKYEELLVNPTLLTLVRQRGNIDCGGLDYVVIRYGSFFEFVAPLDQGHVSVGLEPSAD
                     PLPLVPRILEVVSALLPAAETLSARRGR"
     gene            complement(2168..2371)
                     /locus_tag="VFU45_06475"
     CDS             complement(2168..2371)
                     /locus_tag="VFU45_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018766546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5358743.1"
                     /translation="MDNGIRALRFQAGEMTQQELADRVGVTRQTINAIEGNKYSPSLE
                     VAFRIARVLNVPLEQAFTFHDGR"
     gene            complement(2395..2808)
                     /locus_tag="VFU45_06480"
     CDS             complement(2395..2808)
                     /locus_tag="VFU45_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358744.1"
                     /translation="MGMTFREKGLWLVGSSLVLVYGLYFWRVLPAAGTDVLPSQVGAF
                     AGAVVLLVVIQAAGHALIAIQDRRTAPDERDRLIGLIGERNGAFTLAAGVFLSLGLAV
                     FTRGNFLFTHVLLACWVLASLVSIGTSLWLYRRGA"
     gene            complement(2945..3466)
                     /locus_tag="VFU45_06485"
     CDS             complement(2945..3466)
                     /locus_tag="VFU45_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358745.1"
                     /translation="MHRSHSLVIAALVAAAAPLSAQAPTLHQLQGAWRLVDAISYDSA
                     GHAGAPLRAPGLVLITGRYISRNWTDTTGQAPTFARPWAPTDSEKARRYSAFLGVAGR
                     FTLSADTMIIRPVSARVPEYEGGYSRELVALRGDTLSLTMIAIVSSTGAPVHYYVAGG
                     KQELRFVRAGPRP"
     gene            3611..4171
                     /locus_tag="VFU45_06490"
     CDS             3611..4171
                     /locus_tag="VFU45_06490"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU5358746.1"
                     /translation="MPHIALLRGVNVGGHRVFRPAQLPAQLPELGLVNIGAAGTFVVQ
                     KRVSRERVRAAFAKALPFATAIVICDADEIRRLAAADPLAEYAGQADITRFVMVLGGA
                     PDVEPILPVEFRAGRRWLIRVIGRTGPFALGVYRRDPKTIGLLGRVDRLFDVPVTTRN
                     WNTIGAIVKVLDATGGVPGPIDLEAT"
     gene            4171..4530
                     /locus_tag="VFU45_06495"
     CDS             4171..4530
                     /locus_tag="VFU45_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358747.1"
                     /translation="MPTEPALTPRERVLLHQIHPLKLATDWGTAVAAAYLLWQHHLGA
                     ALRVGLVPPVVVTLALLRWADLGPLGASAFGRYILRHMTRAMEALRFDGLALAWLAAW
                     TRGAITFGERNAGANHH"
     gene            4508..5005
                     /locus_tag="VFU45_06500"
     CDS             4508..5005
                     /locus_tag="VFU45_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU5358748.1"
                     /translation="MPGRTTTDLQDPALLRRLLRAKDRMDAASDEAWPVARLAKVSGV
                     SAAHFARSFRQAFGVPPHRYLLTRRIERATALLRDTDLPIIEIAFRTGWESLGTFGRT
                     FRDITGESPSAVRRQGRAPARQAGPVPACVLKAAHRPHLTTAVSEKRRKRSRGMIGSP
                     RQETR"
     gene            5002..5409
                     /locus_tag="VFU45_06505"
     CDS             5002..5409
                     /locus_tag="VFU45_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5358749.1"
                     /translation="MTQGIEVAGLYVHDQDEALDFYVGKLGFKVHTDVRNGDYRWLTV
                     QHPEQPSFQLGLFAPGPPVLDDATAQALRAIVAKGAMPPLVLTVADCRAAYERLKGQG
                     VEFIQEPIERYGTVDAGFRDPSGNGWKMIQARR"
     gene            5410..5817
                     /locus_tag="VFU45_06510"
     CDS             5410..5817
                     /locus_tag="VFU45_06510"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5358750.1"
                     /translation="MAPKRAPAGAGAGRPAAELIDARIAEFPDWRGKLLARLRALVRA
                     ADPGIIEEWKWEVPVWSHHGIICTGEVYKQVVKMTFARGASLKDPAGLFNSSLEGATR
                     RAIDFREGEKLDERALKALVREAVALNRSRARD"
     gene            5821..6333
                     /locus_tag="VFU45_06515"
     CDS             5821..6333
                     /locus_tag="VFU45_06515"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEU5358751.1"
                     /translation="MSTLSFRARIRIIGVNPYVLVSAARAAALKPGWRRPLPVRIRID
                     GHPRTPWRINLMPRGDGSFYLYLHGSVRQASGTTVGDVVAVEIAFDAGYRGGPAQGIP
                     PWFRKPLAANTRAAAAWKALAPSRQKEIVRYLVRLRSPEARARNSAKALAALSGKPVR
                     FLARSWSGGR"
     gene            6414..7097
                     /locus_tag="VFU45_06520"
     CDS             6414..7097
                     /locus_tag="VFU45_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358752.1"
                     /translation="MTRPAFAALTALLAAAPLAAQQPRDFGPYLTADRAAEIALARTA
                     APRNVTDSATILVMTRTGFVEAVHGTNGFTCAVFRSFAAPSNDPNLWNVKISAPQCFN
                     APATRTVLPMWLKRMEWILGGATPAEADARTARAYASHKFPAPAPGSMAYMLSHEQYL
                     SDSAPHYWKPHLMFFYDRSLPGAAFGADDGGPVIDGSGAVSDTPMVTILIPVPHWSDG
                     TDALPRPAQ"
     gene            7094..7975
                     /locus_tag="VFU45_06525"
     CDS             7094..7975
                     /locus_tag="VFU45_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358753.1"
                     /translation="MSRPARWTILGAALTASAVLAGRPGVRGDPAPTAVTLDGNLDAR
                     TDDSPAFTPDGATAFFDRTAGRSKTIVMTTRIDGHWTPAVTAPFSGQWLDPDPALAPD
                     GSALVFSSNRPVHNDGQPVVFTDDGGKAYRGANLWKVARQGEGWGTPEWLGPAVNTTT
                     LVVAPSVAQDGSVYFIQRERGAMHIFRSDFRDGKYLPARLVALGDTTLPTHDPAIAPD
                     RSFLVFDYGQSSGGLGRLSIAFRQGDGWSAPHDLGDAANSDAPWGAHLGPDARTLYFT
                     GNTHIWRLSLAPWLPAD"
     gene            8076..9242
                     /locus_tag="VFU45_06530"
     CDS             8076..9242
                     /locus_tag="VFU45_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358754.1"
                     /translation="MSHIESAPAPSASLWRRTVVLLTLLALSGCVTIRYQRPGSEVTP
                     RTGETLVFGRVRFFHDGREFFPWKVSLVGDPVATNTERHLWLLRLGRRAVSSEVHPDP
                     DGSLAIWLGSGDYALLGSTEIATEGAPPYEVVALIRVPAGPVSAYAGDLELRTESHEG
                     GHLSRGEFGDATVTLAPITIARATVEQRLGRLPEVPATSSWCAGERVPGFNDSKLAAR
                     AKELLDQGCGAAAVQPPAPADTNDPARVALYGTGDTVLGYVTLGVSTTADAARALRSL
                     GGLGPPRGTDITFTVGSATIHPALIFTPPGTMHQLYFDRDTLVLVVSGIPRGIPATRS
                     EFLARYPGARETRREPGWYELQAPLGRCIWLIAVFTPGTDAIESVGQARACPAS"
     gene            complement(9289..>9644)
                     /locus_tag="VFU45_06535"
     CDS             complement(9289..>9644)
                     /locus_tag="VFU45_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU5358755.1"
                     /translation="LNICTELWALETYRDYAAQGAQLILSPRATAAATTPKWLALGSV
                     AAVRSGAFSVSSNRSDPSGTCGGVGWIISPDGELLATTSATRPFATQDLDLAAPVRAR
                     HSYPRDVFRRSACRR"
BASE COUNT         1412 a         3583 c         3277 g         1372 t
ORIGIN      
        1 ttcgcagaag gcttcgccgg tgatcccggc tggcacgtgc agctgatgaa cgagaccgtc
       61 gacgtggccc ggagcgggga cctggcggtc taccgcggcg tgtatcacga ggaccacggc
      121 gcccctggcg cgctgctgac ccaccggacc aacttcatcg cggaattccg gcggcaacac
      181 gaccgatcct ggaagatcgc gtggtacagc gtgatcagga tggagccgtc gcatccgaag
      241 tgatgggcga cggccggcgc cgcctggcca ggggcgcccg cgcaccacac tgcttcgact
      301 gcgccggtcg atcgtgatcg caggagcgat cgtggtcgcc ctgcccctcc tggccgcggc
      361 cgctttcgtc gccagcacca cgctcctcga ccgcacgaac gggacgctca tctcctccgg
      421 cgtcgagcgc cggtacctgc tcttcgtccc gcccacctac gacccgtccc gccccacccc
      481 gctggtcatc agcctccacg gcgccgccgc gtggccggcg cagcagatgc acctgacgca
      541 ctggaacgat gtcgccgccg agcacggctt catcgtggtc tatccggcgg cgcgggggcg
      601 catctggcgc gtgcagcatc ccgggagcga ccccacggcg gacgtgcggt tcatcggcga
      661 tctcatcgac accctcgagc ggacctatca catcgattcg caccggatct acgccaacgg
      721 cttttcgctc ggcggcgcga tggccttcgc gctcggctgc cggctctccg gccggctggc
      781 ggcggtgggg accgcgtcgg ccgcagaggc cctcccctgg tcgtggtgca cggacgaccg
      841 cccgatgccg ttcatcaatt tccacggcac cgccgacctg gtgccctatg ccggcggccc
      901 ctcgcccgac ccgttcaacc cggtcacctt ccccgccgtg cggcagtgga cggcccagtg
      961 ggccgggcgc aaccggtgcc gcggccaggg cgattcgacc ctggcacccg acgtcgtcgc
     1021 gaccgcgtac cgggactgcg ccgacgacgc gtccgtcgtg ctctacaccg tgcgtggcgg
     1081 cggacacgcc tggccgggcg gcaaggctct gccgcggtgg ccgttcgggc ggacgacgac
     1141 gtccatcgac gcgacgcgga tcatgtgggc gttcttccag gcgcatccgc tggggccgac
     1201 cacgtcgggg cggtcggtgc cgccccggga ggaacattga caatgcccga tcgcccgaga
     1261 cccgccggct actccggcat gccactcgcc cggaagctcg gcatccgcga gggagcgcgg
     1321 atcctgtccc tcggcgcccc cgacggctac gccaagctcc tggggccgct ccccgacgac
     1381 gcaaggattg tcgcgaggct ctcagcggcg gtggacctcg tgcacctctt caccacccgg
     1441 cgcacggagc tcacgaggga gctggcgcgg ctccggcgcg ccatccggcc ggacgcggcc
     1501 gtctgggtgt cctggccaaa gaaggccgcg cgcgcggaca ccgatgtgac ggaggacgtg
     1561 atccgcgcgg tcgcgctgcc gctcgacttc gtggacatca aggtgtgcgc cgtcgacgag
     1621 cgctggtcag ggctcaagct cgtcatccgg aaggccgccc ggccagcgtc gccgcgtgat
     1681 ggggcgcgcc ggtaacgccg gcgtttccca ggagcccggc ctcctcggcg cacgttggcg
     1741 cacgaccggc ccagacggta cattggcgcc gacccttccc atcggtcggc gccacagatg
     1801 cccgcttccc ccgtcgccca gcgcatcctc acactctccc cggccgtccg ctacgtcgcg
     1861 atctaccatc gcggcaccct caccaccgcc acgcgccctg acctggccaa cgccagcagc
     1921 tccgaatcgg acaagtacga ggagctcctg gtcaacccga cgctcctcac cctcgtccgc
     1981 cagcggggga acatcgactg cggcgggctc gactacgtag tcatccgcta cggcagcttc
     2041 ttcgagttcg tcgcgccgct cgaccagggg cacgtgtcgg tcgggctcga gccgtcggcg
     2101 gacccgctcc cgctcgtccc gcgcatcctc gaggtcgtat cggcgctgct gccggccgcc
     2161 gagacgctca gcgcccgtcg tggaaggtga acgcctgttc gagcggcacg ttgagcaccc
     2221 gcgcgatgcg gaacgccacc tcgagcgacg gcgaatactt gttcccctcg atcgcgttga
     2281 tggtctggcg cgtcaccccc acccggtccg ccagctcctg ctgcgtcatc tcgccggcct
     2341 ggaagcggag ggcccggatg ccgttgtcga tgtggctctt gcccttgccc atcgctacgc
     2401 gccgcgccga tacagccaga gcgacgtgcc gatcgacacc agcgacgcca gcacccagca
     2461 cgccaggagc acgtgggtga acaggaagtt cccgcgcgtg aacaccgcca gccccaacga
     2521 caggaacacc cccgcggcca gggtgaacgc gccgttccgc tcgccgatga ggccgatcag
     2581 ccggtcgcgc tcgtccggcg ccgtgcgccg gtcctggatc gcgatcagcg cgtgacccgc
     2641 cgcctgaatg accaccagca gcaccacggc gcctgcgaac gcgccgacct ggctcggtag
     2701 cacgtcggtg ccggccgccg gcaggacccg ccagaagtac agcccataca ccagcaccaa
     2761 ggacgacccc accagccaga ggcccttctc ccggaacgtc atccccatcg ctcagtctcc
     2821 caattggatg tcaagtgttt acgacacaat gacaaaaata cctgacatca ctcgggaagt
     2881 caagtacgcg ggggagatcg ccgaatgcca ttcgtccgga cgcgaccggc gtatgtcctg
     2941 cggcctaggg tcgaggcccc gcccgcacga accgcaactc ctgcttcccg ccggccacgt
     3001 agtaatgcac cggcgcgccg gtggaggaga cgatcgcgat catcgtgagg gacagggtgt
     3061 cgccccggag ggcgacgagt tcgcgggaat agccgccttc gtactcggga acgcgggcgg
     3121 agaccgggcg gatgatcatc gtgtcggccg agagggtgaa gcggccggcg acgccgagga
     3181 aggcgctgta gcggcgggcc ttttcggagt cggtgggggc ccagggacgg gcgaaggtcg
     3241 gcgcctggcc ggtggtgtcg gtccagttcc ggctgatgta cctgccggtg atgaggacga
     3301 ggccgggagc gcggagcggc gcgccggcgt ggccggcgga gtcgtaggag atggcatcga
     3361 cgaggcgcca ggccccctgc agctggtgga gggtcggcgc ctgcgcgctg agcggcgcgg
     3421 cggccgcgac gagcgcggcg atgacgagcg agtgcgagcg atgcatcagg cgatcctcga
     3481 gggttgggcg ccacggcgtt cccgcgacca tattgctcgt acgcacggtc gaccggcgcc
     3541 agtttcaccc cgtccgccca acgaccaacg accaaagacc agcgacctca cgaccctcga
     3601 ctcacgaccc atgccccaca tcgccctcct ccgcggcgtc aacgtcggcg gccaccgcgt
     3661 cttccgcccc gcccagttgc cggcgcagct cccggaactc ggcctcgtca acatcggggc
     3721 ggcggggacg ttcgtcgtcc agaagcgcgt ctcccgggag cgggtgcgcg cggcattcgc
     3781 gaaggcgctg ccgttcgcca ccgccatcgt catctgcgac gccgatgaga tccgccgcct
     3841 ggccgcggcg gatccgctgg ccgagtacgc cggacaggcg gacatcacgc gcttcgtgat
     3901 ggtcctcggc ggcgcgccgg acgtcgagcc gatcctgccg gtcgagttcc gcgccgggcg
     3961 gcgctggctg atccgcgtca tcggccgcac cggccccttt gcgctcggcg tatatcggcg
     4021 cgacccgaag acgatcgggc tcctgggccg ggtggaccgg ctgttcgacg tgccggtgac
     4081 gaccaggaac tggaacacga tcggcgcgat cgtgaaggtg ctggacgcga caggcggcgt
     4141 gccggggccg atcgacctgg aggcgacttg atgcccacgg agccggcgct gacgccccgg
     4201 gagcgggtac tcctccacca gatccatccg ctcaagctcg ccaccgattg gggcacggcc
     4261 gtcgccgcgg cgtatctcct ctggcagcat cacctcggcg cggcgctccg cgtcggcctg
     4321 gtcccgccgg tcgtggtgac gttggcgctc ctccgctggg ccgacctcgg gcccctcggc
     4381 gcatcggcat tcggtcggta cattttacgc catatgaccc gggcgatgga ggccctgcgg
     4441 ttcgacggcc tcgcgctcgc ctggctcgcg gcctggaccc ggggggccat caccttcgga
     4501 gagcggaatg ccggggcgaa ccaccactga tctccaggac ccggcgctcc tccgccggct
     4561 cctccgcgcc aaagaccgga tggacgccgc ctcggacgag gcgtggccgg tggcgcggct
     4621 cgccaaggtg agcggcgtct cggcggcgca ctttgcccgc tcgttccggc aggcgttcgg
     4681 cgtcccgccg caccgctacc tccttacgcg ccggatcgag cgcgccaccg cgcttctccg
     4741 cgacaccgat cttcccatca tcgagatcgc cttccgcacc ggctgggaga gcctcggcac
     4801 cttcggccgc accttccgcg acatcacggg cgagagcccg agcgccgtcc gccggcaggg
     4861 acgggccccg gcccgtcagg cagggccggt gccggcctgc gtcctcaagg ccgcccatcg
     4921 gccgcatctg acgaccgcag tttcggagaa gcgccggaag cgctcgcgcg gtatgatcgg
     4981 gtccccacgc caggagaccc gatgacgcag ggcatcgaag tggccggcct ctacgtccat
     5041 gaccaggacg aggcgctcga cttctacgtc gggaagctcg gcttcaaggt ccacaccgac
     5101 gtgcgcaacg gcgactaccg ctggctcacc gtccagcacc cggagcagcc gtcgttccag
     5161 ctcggcctct ttgcacccgg cccgccggtg ctcgacgacg cgaccgcgca ggcgctccgg
     5221 gccatcgtgg ccaagggggc gatgccgccg ctcgtgctca ccgtggccga ctgccgggcc
     5281 gcctacgagc ggctcaaggg ccaaggcgtc gagttcatcc aggaaccgat cgagcggtac
     5341 ggcacggtgg acgccggatt ccgcgacccg tcggggaacg gctggaagat gatccaggcg
     5401 cggcgctgaa tggccccgaa gagagcgccg gctggcgcgg gcgccgggcg gccggcggcg
     5461 gagctgatcg acgcgcggat cgcggagttc cccgactggc gcgggaagct cctcgcgcgc
     5521 ctccgcgcgc tggtcagggc ggccgacccg gggatcatcg aggagtggaa gtgggaggtc
     5581 cccgtctggt cgcaccacgg gatcatctgc accggcgaag tgtacaagca ggtggtgaag
     5641 atgacctttg cgcggggggc gtcgctcaag gaccccgccg ggctcttcaa ctcgagcctc
     5701 gaaggggcga cccgccgggc gatcgacttc cgcgagggag agaagcttga cgagcgggcg
     5761 ctcaaggcgc tggtccggga agccgtggcg ttgaatcgat cgcgcgcacg cgactgaccg
     5821 gtgtccacgc tttctttccg cgcgcggata cggatcatcg gcgtcaatcc gtacgtgctg
     5881 gtgagcgccg cgagggccgc ggcgctcaag cccgggtggc gccgccccct ccccgtccgc
     5941 atccggatcg acggacatcc ccgtaccccc tggcgcatca acctgatgcc gcggggggac
     6001 gggtcgttct acctctacct gcacggcagt gtccgccagg cctccggcac cacggtggga
     6061 gatgtcgtgg cggtcgagat cgccttcgac gcgggttacc gcggaggccc ggcgcagggg
     6121 atcccgccct ggttccggaa gccgctcgcg gccaacacca gggcggccgc ggcgtggaag
     6181 gcactcgcac cgagccggca gaaggagatc gtgaggtacc tcgtgcggct caggtcgccg
     6241 gaggcgcggg cgcggaactc ggccaaggcc ttggcggcgc tgtccggaaa gccggtgcgg
     6301 ttcctggcgc ggtcgtggag cggcgggcgt tgacggtgca agggcggacg gggtggggcg
     6361 tcccctgtcg aactggcacc cacgttcccg gtctccatcg gcaggatctc tccatgaccc
     6421 gccccgcatt cgcggcactc acggcgctgc tcgccgccgc ccccctcgcc gcccagcaac
     6481 ccagggattt cggcccctac ctcacggccg accgcgccgc cgagatcgcc ctggcgcgga
     6541 ccgcggcacc gaggaacgtg accgattccg cgaccatcct ggtgatgacc cgcaccggct
     6601 tcgtcgaggc ggtgcacggg acgaacggct tcacctgtgc ggtcttccgg tcctttgcgg
     6661 ccccgagcaa cgacccgaac ctctggaacg tgaagatcag cgcgccgcag tgcttcaacg
     6721 cccccgccac gcggaccgtc ctcccgatgt ggctcaagcg gatggagtgg atcctgggcg
     6781 gcgccacccc tgccgaagcc gacgcacgca ccgcgcgggc ctacgcctcg cacaagttcc
     6841 ccgcgcccgc ccccggctcg atggcctaca tgctctcgca cgagcagtac ctgagcgact
     6901 ccgctccgca ctactggaag ccgcacctga tgttcttcta tgaccggtcg ctccccggcg
     6961 ccgccttcgg agccgacgac gggggaccgg tcatcgacgg ttccggtgcg gtgtcggaca
     7021 cgccgatggt gacgatcctc atccccgtgc cgcactggtc ggatgggacg gacgcccttc
     7081 cgcgccctgc gcagtgagcc gcccggcacg atggaccatc ctgggcgcgg ccctgaccgc
     7141 cagcgcggtg ctcgccggcc gtcccggcgt gcggggtgat ccggcaccca cggccgtgac
     7201 cctggacggg aatctcgacg ccaggaccga cgattccccc gccttcaccc ccgacggcgc
     7261 cacggccttc ttcgaccgga cggcgggccg tagcaagacg atcgtcatga caacgcggat
     7321 cgacggccac tggactcccg cggtgacggc gcccttctca ggccaatggc tcgacccgga
     7381 tccggcgctc gcgcccgacg gatcggctct cgtcttcagt tcgaaccgcc cggtgcacaa
     7441 cgacggccag ccggtggtct tcacggatga cggcggcaag gcgtaccggg gtgccaatct
     7501 ctggaaggtg gcgcgtcagg gcgagggctg gggcacgccc gaatggctcg gccccgccgt
     7561 caacacgacg acgctggtcg tggcgccgag cgtcgcgcag gacgggagcg tctacttcat
     7621 ccagcgggag cggggcgcga tgcacatctt ccgctccgat ttccgggacg ggaagtatct
     7681 ccccgcccgg ctggtggccc tcggcgatac gaccctcccc acccacgacc ccgcgatcgc
     7741 gccggatcgg tcgttccttg tcttcgacta cggccagtcc tccggcgggc tcggccgcct
     7801 cagcatcgcc ttccgccagg gggacggctg gtccgccccg cacgacctgg gtgacgcggc
     7861 caacagcgac gcaccctggg gcgcccatct cgggccggac gcccggaccc tctacttcac
     7921 cggcaacacc cacatctggc ggctgtccct ggcaccgtgg ctcccggcag actagcgccg
     7981 cccgggcatc acggaaccct catctgccct tcacggtgac ccgcccccct ccctgctagc
     8041 gtgtcctgcg gacacccgcc ggaccaggag ggaacgtgag tcacatcgaa tccgcccccg
     8101 cgccatcggc gagcctgtgg cgccggacag tcgtgctttt gaccctgctg gcgctgtcgg
     8161 gatgcgtgac gatcaggtac cagcgccccg gcagcgaagt gacgccgcgc acgggagaga
     8221 cgctggtatt cgggcgggtg cgcttcttcc acgacggccg cgagttcttc ccgtggaagg
     8281 tgagcctcgt gggcgatccg gtcgcgacga acaccgaacg ccacctctgg ctcctccgcc
     8341 tgggacgccg cgccgtgtcg tcggaagtgc accccgaccc cgatggctcc ctcgccatct
     8401 ggctcggcag cggggactac gccctcctcg gcagcaccga gatcgcgacc gagggcgcgc
     8461 caccgtacga ggtggtggcg ctcatccgcg tgcccgccgg cccggtctcg gcctacgccg
     8521 gcgacctcga actgcggacc gagagccatg aggggggaca cctttcgcgc ggtgagttcg
     8581 gcgacgcgac cgtcaccctg gcaccgatca ccatcgcccg ggcgacggtc gaacagcgcc
     8641 tcggaaggct cccggaagtg cccgcgacgt cgtcgtggtg cgcgggggag cgcgtgccgg
     8701 ggttcaacga ctccaagctc gcggcccgcg ccaaggagct cctggaccag gggtgcggcg
     8761 cggccgcggt ccaaccacct gcgccggccg acacgaacga ccccgcccgg gtcgcgctct
     8821 atggtaccgg cgatacggta ctcggatacg tcaccctcgg cgtatcgacc accgcagacg
     8881 cagcgcgagc cctccgatcg ctgggcgggc tcgggccgcc gcgggggacc gacatcacgt
     8941 tcaccgtcgg ctctgcgacg atccacccgg cgctgatctt cacgccgcca gggacgatgc
     9001 accagctcta tttcgaccgc gacacgctgg tgctggtcgt ctccggcatc ccccgcggca
     9061 tcccggccac gcgaagcgag ttcctggccc ggtatccagg agcgcgagag acgcgccgag
     9121 aaccgggatg gtacgaactg caggcgccgc tcggccgctg catctggctg atcgccgtgt
     9181 tcacccccgg cactgacgcg atcgaatcgg tgggccaggc gcgcgcctgc ccggcgtcgt
     9241 agtacaccgg ctccctgcac ggcgtgcgac ccggtccctg acccggcctc acctgcgaca
     9301 cgccgaacga cggaacacgt ccctcggata ggagtgccgc gcccgcaccg gggccgccag
     9361 gtccaggtct tgggtggcga acggccgggt ggccgaagtg gtggcgagga gctcgccatc
     9421 ggggctgatg atccacccca ctccgccgca ggtcccgctg gggtcggacc ggttcgacga
     9481 cacgctgaac gcgccgctcc gcaccgccgc caccgaaccc agcgccagcc acttcggggt
     9541 cgtcgcggca gcggtcgcgc ggggcgagag gatcagctgg gcgccctgtg ccgcgtagtc
     9601 acggtacgtc tcgagcgccc agagctcggt gcagatgttc aacc
//
LOCUS       DASUAP010000249        12591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_361360, whole genome shotgun sequence.
ACCESSION   DASUAP010000249 DASUAP010000000
VERSION     DASUAP010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 12591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12591
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361360"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFU45_06540"
     CDS             complement(<1..466)
                     /locus_tag="VFU45_06540"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358756.1"
                     /translation="MPIDLGHHPPIVWGGFTLVVAVLLVLDLFVVNRKSHVLTLAEAA
                     RWFGALVALALLFGLFLLGREGPRAMLEYYTGYLIELSLSVDNLFVFILVFQYFAVPA
                     ALQPKVLKWGILGALVLRAAMILGGVLLLQRFSWITWVFGAILVFTGWKMFTR"
     gene            complement(515..1777)
                     /gene="glp"
                     /locus_tag="VFU45_06545"
     CDS             complement(515..1777)
                     /gene="glp"
                     /locus_tag="VFU45_06545"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU5358757.1"
                     /translation="MTDVPSHLSAREAAMRVVAGVRCQPPLRVPLDDALGMVLADDVR
                     SPVDVPPRTSSSMDGYAARAADVRGATAERPAVLRLVERIPAGSVPTRALGPGECARV
                     FTGSPVPAGTDTVVRQEDAAADGEHVRIVTDRDAGANLRQPGEDIRRDAVALTAGLPL
                     RAAHLGVLASLGIGAPMVVRRPRVAILCCGDEVADVDRPDEIQDGRRIGNASGHALRA
                     LIRQAGGEPVDLGIASDTLGSIRDHLARAADADLVVTAGGISVGDHDQVRAAVQASGG
                     AVDFWRVRIRPGGPVAAGSVAGIPWVGLPGNPVSAMVTCELFVRPAIRKMAGHAEPFR
                     RCRPVRTAAAISLRPTLQHFLRAVVSGDGEGREARLTGPQGSGILSSMAAANALLIVP
                     EGQHETPAGAGLSALLLDEAEHQGEPGF"
     gene            complement(1859..2671)
                     /locus_tag="VFU45_06550"
     CDS             complement(1859..2671)
                     /locus_tag="VFU45_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358758.1"
                     /translation="MSPAATARAACILLLAGAAAPLRAQATADRARLAVGLGIAYHGG
                     GHIWTQDGQTIIDQSANVDTATVGRDIQSQLGLSFMGAYFPTERWGVIGEVQFLGLHY
                     VDSCRLLTNHSPDEAPQICRNLQGRGHDGTAVTLTLGGIFRPWPTSEVSPYLRASGGL
                     NMSQNSSVRMIGTWITSTFEEADYYIFGDPSPQEIMPTLALGGGITAPTGRGSQFRLE
                     ARDNVVFLRHPVGPQSTPDPGLTPPTKVRASHLFSLNLSFEIVLEKRRGRRY"
     gene            complement(2668..3090)
                     /locus_tag="VFU45_06555"
     CDS             complement(2668..3090)
                     /locus_tag="VFU45_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5358759.1"
                     /translation="MSVPEPTLEIVRSARIIAIVGLSPDAARPSHGVGTYLQAQGYRI
                     LPVTPRGGPILGQPAFPDLPTAAAAVAPERIDIVDVFRRPEHVAALLPDILAVHPKLV
                     WLQLGIRDDATAAKLEAAGISVVQDHCLGVEHSLRIRR"
     gene            complement(3128..3538)
                     /locus_tag="VFU45_06560"
     CDS             complement(3128..3538)
                     /locus_tag="VFU45_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU5358760.1"
                     /translation="MRILLTRTVAFTARHRLAFQDRSAEENRRRFGALAEPHEHRYTC
                     AVTVEGAPGPGTGMVMDLVELDRILAEEVTRRLGGTDLNQSLDAARAGTALPVCETIA
                     SELFGRIGGRLPPGVSLDRVRIEEAPDLAAECRA"
     gene            complement(3549..4247)
                     /locus_tag="VFU45_06565"
     CDS             complement(3549..4247)
                     /locus_tag="VFU45_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358761.1"
                     /translation="MNRYAALLLAATIMARPISAQVGHAPDSSPYRDIIHPNGLLFTY
                     GHIAGDGGSLGVGPHNGDSWGLRYDVRLSGLLGAHLGFSHFSGVRDAYYPDDSVAKRR
                     TGPYSQSVTFIGVGMQANLTGPKTWHRLAPFVDAELGAAVGSKVAADTASLYDFGTKL
                     YLDPGVGVQWFVTSRLRIRFDARRVYWKLKYPVSFFAAPTAQPTASPIIPAGGSATEW
                     SGNGWLSLGIHYSL"
     gene            complement(4253..5806)
                     /gene="gpmI"
                     /locus_tag="VFU45_06570"
     CDS             complement(4253..5806)
                     /gene="gpmI"
                     /locus_tag="VFU45_06570"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEU5358762.1"
                     /translation="MSNNARPAGPVVLIVLDGWGYRAEREGNAIELARTPVWHRLWEA
                     GPRTLLDASGLAVGLPAGQMGNSEVGHLNLGAGRVVMQDLVRIDQSIASGAFFQLEPL
                     RALAAHLKRTGGTLHLIGLIGSGGVHAIDRHLVAALEFAHRAGVPRIAVHGFLDGRDS
                     PPTSALGFVRELLGDIARIGGDRACLASVTGRYFAMDRDKRWDRTQLAYDAMVRGLGA
                     PATDVLAAIQASYDVGVTDEFVRPIVVRRDGAPAATLRDGDAVFCFNYRADRMRQICH
                     ALVDDPFTGFDVTGRPKLALATMTQYDRTLQAPQAFPPMSMARIMAEVLADAGRAQLR
                     TAETEKYAHVTYFFNGGYEPPYKGEERILVPSQKVATYDLAPEMSAAGITDTLCAAIE
                     AHHHDFILCNYANADMVGHTGVAPAIITAVETVDACLARVLASAERARAQVLITADHG
                     NCELMVDPVTGGPHTAHTTNPVPLVTVGAGPAPLRAGGALCDVAPTLLRMMGIDPPAD
                     MTGRDLRLT"
     gene            5837..6940
                     /locus_tag="VFU45_06575"
     CDS             5837..6940
                     /locus_tag="VFU45_06575"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU5358763.1"
                     /translation="MTYRPQSSPGPRRQPAFPALSRALLAGLLALSLPGVAAAQDHRT
                     LARESRIYATPGGASLGLVTRGTSLPSRRVDGTAVELQVDGWVFARSVGAADRDGFDL
                     SVTQPGGQNLRESPTPGARIVARLARGALLQKVEADGHGWVHVRRYAWVPGAALQPLP
                     AASAPAPAVAARPSPSAAAARPDSGGGTPAPLPPVQLSPSDRQQMARAAALTVGPGGA
                     TAASLPQGSEVKVVTRAGDWTQVSVTGWVRTSDLAPLPDQAMVGVSAAEVRADPARYI
                     GRVVEWRLQFVSVMVADELRPEIPAGRSYLLTRGPLPESGFVYVVLPPDQVERFRTLE
                     PLTELTLRVQVRAASTKFLPNPVVDLLQVVTGR"
     gene            6956..7294
                     /locus_tag="VFU45_06580"
     CDS             6956..7294
                     /locus_tag="VFU45_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358764.1"
                     /translation="MHEQLKERIARRLESLADERGYQVLDYIEFLESKYGERSTPTNF
                     FARLAERVEDTLRAGRIPVKAIGGTLTAMDAAGKVMKGVVAAGKAVVDEAVSAANAPG
                     AIGPPKDPPK"
     gene            7393..8871
                     /locus_tag="VFU45_06585"
     CDS             7393..8871
                     /locus_tag="VFU45_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5358765.1"
                     /translation="MTTDSPSTDFIRGIGPLQAVSLVVGSMIGSGIFIVSADIGRQVN
                     AWGPGGLMLVWVLTGLMTVAGALSYAELAGMMPKAGGQYVFLREGLNPLAGFLYGWTL
                     FAVIQTGTIAAVAVAFAKYLGVFFPALTDHLFLPLGSFALPAGLGGQSIALGLSPERV
                     VAILSVLFLTWVNVRGVGLGAGIQTLLTIVKTVALGGLILLGITIFRHADVWAANVAH
                     FWGTAPWSLAMLPVIAAAMVGSLFSSDAWNNVTFAAAEVREPARNLPRALALGTGLVT
                     LLYLLANVAYLHVLPFAGDPAGADALARGMQHAASDRIGAAAAEVMLGSAGALVMAGL
                     IVISTFGCNAGLILSGPRLYWAMAQDKLFFAPAAALHPVHRTPTFGLWAQAVWTSLLC
                     LTGTYNQLLDFVIPAVLVFYFLTIIALFRLRATRPDLPRPVKAFGYPVVPGLYLVALA
                     FVTGAQVLARPLFAATGLIIVAIGVPVYMWWHRGAATTRRDS"
     gene            9127..10590
                     /gene="guaB"
                     /locus_tag="VFU45_06590"
     CDS             9127..10590
                     /gene="guaB"
                     /locus_tag="VFU45_06590"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU5358766.1"
                     /translation="MSAPRVRPTPALTFDDVLLAPRHSLVHPKDVATASWFTRKIPLQ
                     IPFISAAMDTVTEAEMAIAMARAGGIGVIHKNMTVERQAAEVDRVKRSESGMILNPIT
                     LGPDRPLREAAQLMSRFRISGVPIVDGQGRLIGIVTNRDLQFERNLDQPISEAMTKDK
                     LVTAPVGTGLDAAERILARHRIEKLPVVDEAGVLKGLITVKDIFKRKQHPKSNKDQHG
                     RLRVAAAVGGSPAAVDRARALLDAGVDALVVDSAHGHSEGVLRTVDALRLKFPDAQIV
                     AGNVATEAGARELVRRGVDAVKVGVGPGSICTTRVVTGVGMPQVTAVMDAVRGAGEVP
                     VIADGGIKYSGDAVKALAAGACSVMMGAMLAGTEESPGESVLAEGRRYKMIRGMGSMS
                     AMQDGSADRYFQEGEMAPSKFVPEGIEGRVPYRGPVGDVLFQMVGGLRSGMGYCGVGD
                     IEALRTQVEFVQITTAGLRESHPHDVTITREAPNYSA"
     gene            10661..12145
                     /locus_tag="VFU45_06595"
     CDS             10661..12145
                     /locus_tag="VFU45_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5358767.1"
                     /translation="MSEPEAKGAPLPAHGAGIWRRKSITELQVEAETDHSLKRALGAV
                     NLTALGIGAIIGTGIFVLTGTVAAINSGPAVVLSFVLAGVASIFAALCYSEFASLVPM
                     AGSAYTYGYATLGELIAWIIGWDLVLEYALGAVTVAIGWSGYVVSFLDGIGIHIPAAL
                     TAARGQIVTLPDGSTVTAIFNLPAVLIIAVVTTLLVVGIKESATVNNAIVVIKVAVVL
                     LFIVLAAKAINPANWHPFIPPNTGVREQFGWTGVMAGAGVVFFAYIGFDAVSTAAQEA
                     KNPQRDMPIAIIGSLLICTLLYIVVSGIATGVMPYQQLNVPDPIAVVADRAGLGWISG
                     LIKLGAIAGLSSVILVMLLGQSRVFWTMSKDGLLPPFVAKVHPKFHTPWITSIVTGVG
                     VAIFSAILTVREAGSLVSIGTLLAFVIVSIGILVLRVREPGLPRPFKAPVVWFTAPMG
                     ALSALYLMISLERRTWERLIIWFVLGMFVYILYSRHHSKLRSRS"
     gene            12328..>12591
                     /locus_tag="VFU45_06600"
     CDS             12328..>12591
                     /locus_tag="VFU45_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5358768.1"
                     /translation="MQLLRRKSVTALQAEAEADHSLKRVLSATQLTMLGIGAIIGTGI
                     FVLTGTVAAENAGPAVVLSFGLAGFASIFAALCYSEFASLVPMA"
BASE COUNT         1714 a         4499 c         4532 g         1846 t
ORIGIN      
        1 tgcgggtgaa catcttccag ccggtgaaca ccaggatggc gccgaacacc cacgtgatcc
       61 aggagaagcg ctgcagcagc aggacgccgc ccaggatcat ggccgcccgg aggacgaggg
      121 cgccgaggat cccccacttg agcaccttcg gctggagcgc ggccgggacc gcgaagtact
      181 ggaagacgag gatgaagacg aacaggttgt cgacgctgag gctgagctcg atcaggtatc
      241 cggtgtagta ctcgagcatc gcccggggtc cctcgcgccc gagcaggaag aggccgaaga
      301 ggagcgccag cgccaccagc gccccgaacc agcgcgccgc ctcggccagg gtgagcacgt
      361 ggctcttccg gttgaccacg aagaggtcga ggaccaggag caccgccacg acgagcgtga
      421 agccgcccca gacgatgggc gggtggtgtc cgaggtcgat gggcatgcgg aaaggtagag
      481 cggggaagga gaggggagaa gccgaagaca cgcgctagaa gccgggctcc ccctggtgtt
      541 cggcctcgtc cagcagcagg gccgaaagcc ccgcgccggc gggcgtctcg tgctggccct
      601 cgggcacgat gagcagtgcg ttcgccgcgg ccatcgagct gaggatcccc gagccctgcg
      661 gccccgtcag gcgcgcttcc cgcccctcgc cgtcgccgga gaccaccgcc cggaggaagt
      721 gctgcagcgt cggccggagg gaaatggcgg cggcggtccg gaccggccgg caccggcgga
      781 acggctccgc gtggcccgcc atcttccgga tggccggccg gacgaagagc tcgcaggtga
      841 ccatcgcgct caccgggttg ccgggaagcc cgacccacgg gatgcccgcg acgctcccgg
      901 cggcgacggg acccccgggg cggatccgca cgcgccagaa gtccacggcg ccgcccgacg
      961 cctgcacggc cgcgcgcacc tggtcgtgat cgccgacgct gatgccgccg gcggtgacga
     1021 ccaggtcggc gtcggcggcg cgggcgaggt gatcccggat gctcccgagg gtgtccgacg
     1081 cgatgccgag gtccaccggc tccccgcccg cctgccggat gagcgcccgg agcgcgtggc
     1141 cgctggcgtt cccgatccgg cggccgtcct ggatctcatc ggggcggtcg acgtccgcca
     1201 cctcgtcacc gcagcacagg atggcgaccc gtggccggcg caccaccatc ggcgccccga
     1261 tcccgagcga ggcgaggacg ccgaggtggg ccgcgcggag cggaagaccg gcggtgagcg
     1321 ccaccgcgtc gcgccggatg tcctcgccgg gctggcgcag gttggcgccc gcgtcgcggt
     1381 cggtcacgat ccggacgtgt tcgccgtcgg cggcggcgtc ctcctggcgc accacggtgt
     1441 ccgtgcccgc tggtaccgga ctgccggtga acacccgcgc gcattccccg gggcccagcg
     1501 ctcgcgtcgg cacgctgccg gcggggatcc gctccaccag gcgcagcacg gcggggcgtt
     1561 cggcggtggc gccgcgcacg tccgcggcac gtgcggcata cccgtccatc gagctgctgg
     1621 tacggggcgg cacgtccacg gggctccgta cgtcgtccgc cagcaccatc ccgagcgcgt
     1681 catcgagcgg cacgcggagg ggcggctggc agcgcacgcc ggcgacgacg cgcatcgccg
     1741 cctcacgggc ggagagatgg gacgggacat ccgtcatcgg gggagcgtcc ggtgggtggc
     1801 gggcacgtcg gggaggatac cggcgccggc cgccgggcgg aagctcggcg cggggccgtc
     1861 agtatcgccg gccgcgccgc ttttcgagga cgatctcgaa gctcaggttg agggagaaca
     1921 ggtgggaggc gcggaccttc gtcggcgggg tcagccccgg gtcgggggtg gactgggggc
     1981 cgacggggtg ccgcaggaac acgacgttgt cgcgcgcctc cagccggaac tgggagccac
     2041 ggcccgtggg cgcggtgatc ccgccgccca gcgccagcgt gggcatgatc tcctgcgggc
     2101 tcgggtcgcc gaagatgtag tagtccgcct cctcgaacgt cgacgtgatc caggtgccga
     2161 tcatccggac cgagctgttc tggctcatgt tgagcccgcc gctggcgcgc aggtagggcg
     2221 agacctcgct cgtgggccac gggcggaaga tgccgcccag ggtgagcgtc acggcggtgc
     2281 cgtcgtgtcc ccgtccctga aggttccggc agatctgtgg cgcctcgtcg gggctgtggt
     2341 tcgtgagcag gcgacaggag tcgacgtagt ggagccccag gaactggacc tcgccgatca
     2401 cgccccaccg ttcggtcggg aagtacgccc ccatgaagct cagcccgagc tgcgactgga
     2461 tgtcgcgccc gacggtcgcg gtgtcgacgt tggccgactg gtcgatgatc gtctggccgt
     2521 cctgggtcca gatgtgcccg ccgccgtggt aggcaatccc cagacccacc gccaggcggg
     2581 cgcggtcggc ggtggcctgc gcccggagcg gcgccgcggc cccggccagc aggaggatgc
     2641 acgctgcgcg ggcggtggcc gcggggctca tcgccggatc cggagtgagt gttcgactcc
     2701 caggcaatgg tcctgcacca cgctgatccc cgcggcctcg agcttggcgg cggtcgcgtc
     2761 gtcgcggatg ccgagctgca gccacacgag cttcgggtgg acggccagga tgtccggcag
     2821 gagcgccgcg acgtgctccg ggcggcggaa cacgtcgacg atgtcgatgc gctcgggcgc
     2881 cacagcggcg gcggcggtcg ggaggtcggg gaaggctggc tggccgagga tggggcctcc
     2941 gcgaggcgtc accgggagga tccggtagcc ctgcgcctgc aggtaggtgc cgacgccgtg
     3001 actcggacgc gcagcatcgg ggctcagtcc cacgatggcg atgatgcggg ccgagcggac
     3061 gatctcgagt gttggttcgg ggacgctcaa gggggccctc cttgcacgaa gtgtccttcc
     3121 ccgcaggtca ggcccggcac tcggcggcga ggtcgggggc ctcctcgatg cgcacccggt
     3181 cgagcgacac gccgggaggg agccgccctc cgatccggcc gaagagctcc gacgcgatcg
     3241 tctcgcagac cggcagggcc gtccccgcgc gggccgcatc gagcgactgg ttgaggtcgg
     3301 tcccgccgag gcggcgggtg acctcctccg ccaggatccg gtcgagctcc acgaggtcca
     3361 tcaccatccc cgtcccgggg ccgggcgcgc cctcgaccgt gacggcgcag gtgtaccggt
     3421 gctcgtgcgg ctcggcgagc gccccgaatc ggcggcggtt ctcctccgca ctccggtcct
     3481 ggaaggccag acggtggcgg gcggtgaagg cgaccgtccg ggtcagcagg atccgcactt
     3541 ggccgggctc acagggagta gtggatgccg aggctcagcc agccattgcc gctccactcc
     3601 gtggcgctcc cgccggccgg gatgatcggg cttgctgtcg gctgagcggt gggcgcggcg
     3661 aagaagctca ccgggtactt gagcttccag tacacccggc gggcgtcgaa gcggatccgc
     3721 agccggctgg tcacgaacca ctggacgccc accccgggat cgaggtagag cttggtgccg
     3781 aagtcataga ggctcgcggt gtcggcggcg accttcgacc ccaccgccgc gccgagctcg
     3841 gcgtcgacga acggcgccag ccggtgccag gtcttcgggc ccgtgaggtt ggcctgcatg
     3901 cccacgccga tgaaggtgac cgactgggag taggggccgg tccgccgctt cgccacgctg
     3961 tcgtcgggat agtacgcgtc gcgcacgccg gagaagtggg agaacccgag gtgggcgccg
     4021 aggagcccgc tcagccggac atcgtagcgc aggccccagg aatcgccgtt gtggggcccg
     4081 accccgagcg aaccgccgtc cccggcgatg tggccgtagg tgaacagcag cccgttcggg
     4141 tggatgatgt cccggtaggg cgagctgtcc ggggcgtgcc ccacctgggc cgagatgggg
     4201 cgcgccatga tcgtggcggc gaggaggagc gcggcgtagc ggttcatgga tctcacgtca
     4261 gtcggaggtc gcggccggtc atgtcggccg gaggatcgat gcccatcatc cggaggagcg
     4321 tcggcgccac gtcgcacagt gcgccgccgg cgcggagcgg ggcgggcccc gcgcccaccg
     4381 tcaccagcgg caccgggttc gtggtgtgcg cggtgtgggg cccgccggtc acggggtcca
     4441 ccatcagttc gcagttgccg tggtcggcgg tgatcagcac ctgcgcccgc gcccgctcgg
     4501 cgctcgcgag cacccgggcc aggcaggcat ccaccgtctc cacggccgtg atgatcgccg
     4561 gggccacgcc ggtgtgcccc accatgtccg cgttggcgta gttgcagagg atgaagtcgt
     4621 ggtggtgcgc ctcgatcgcg gcgcagagcg tgtcggtgat gccggccgcg ctcatctcgg
     4681 gcgccaggtc gtaggtggcc accttctgcg acggcaccag gatccgctcc tcgcccttgt
     4741 agggcggctc atagccgcca ttgaagaagt acgtcacgtg ggcgtacttc tcggtctccg
     4801 cggtgcggag ctgcgcccgg ccggcgtcgg ccagcacctc cgccatgatc cgcgccatcg
     4861 acatcggcgg gaacgcctgg ggggcctgga gcgtccggtc gtactgggtc atcgttgcga
     4921 gcgccagctt cggccggccc gtcacgtcga atccggtgaa cggatcgtcc accagcgcgt
     4981 ggcagatctg gcgcatccgg tcggcgcggt agttgaagca gaagaccgcg tcgccgtcgc
     5041 ggagggtggc cgcgggggcg ccatcccgcc gcaccacgat cgggcggacg aactcgtcgg
     5101 tgacgccgac atcgtaggac gcctggatgg cggcgaggac gtcggtggcg ggggcgccga
     5161 ggccgcgcac catcgcgtcg tacgcgagct gggtgcggtc ccaccgcttg tcgcggtcca
     5221 tcgcgaagta gcggccggtc accgacgcca ggcatgcacg gtcgccgccg atccgggcga
     5281 tgtcgcccag gagttcgcgc acgaagccga gggctgaggt cggcggcgag tcgcggccgt
     5341 cgaggaaccc gtgcacggcg atgcggggga cgccggcgcg atgcgcgaat tcgagcgccg
     5401 ccaccaggtg ccggtcgatg gcgtggacgc cgccggaccc gatgagcccg atgaggtgca
     5461 acgtgccgcc ggtgcgcttg aggtgggcgg cgagcgcacg gaggggttcg agctggaaga
     5521 acgcgccgct tgcgatcgac tggtcgatgc gcaccaggtc ctgcatcacc acgcggccgg
     5581 cgccgagatt gaggtgcccc acctcgctgt tgcccatctg gcccgccggg agcccgaccg
     5641 cgaggccgct cgcgtcgagc agggtccggg gcccggcctc ccagagccga tgccacaccg
     5701 gcgtgcgcgc gagctcgatc gcgttcccct cgcgctcggc acggtagccc cacccgtcca
     5761 gcacgatgag cacgacgggg cccgccggcc gcgcattgtt tgacatggcc ctcccggcct
     5821 tctacaatgc cgaatcgtga cttacaggcc gcaatctagt cccgggccgc gccggcaacc
     5881 agcctttccg gcgctcagcc gcgcgcttct ggcggggctg ctggcgctct cccttccggg
     5941 cgtcgcggcg gcgcaggacc accgcacgct cgcccgcgaa tcccgcatct acgccactcc
     6001 gggcggcgcc tccctcggcc tcgtgaccag ggggacgtcg ctcccctccc ggcgcgtgga
     6061 cggcaccgcc gtcgagctcc aggtcgacgg gtgggtcttt gcccggtcgg tgggtgccgc
     6121 tgaccgcgac ggattcgacc tgtcggtgac acagcccggc gggcagaacc tccgcgagtc
     6181 cccgacgccc ggcgcccgca tcgtggcgcg gctcgcgcgg ggcgccctgc tgcagaaggt
     6241 cgaggctgac ggccacggct gggtccacgt ccggcggtac gcgtgggtcc cgggtgccgc
     6301 gctccagccg ctgccggcgg ccagcgctcc cgcccccgcc gtggcggcgc gaccctcgcc
     6361 gtcggcggcc gcggccaggc ccgactccgg cggcggcacg ccggccccgc tcccgccggt
     6421 gcaactgtca ccgagcgacc ggcagcagat ggcgcgggcg gcggcgctca cggtcggccc
     6481 gggcggcgcg accgcggcct cgttgccgca ggggtcggag gtgaaggtgg tgacgcgtgc
     6541 gggggactgg acccaggtct cggtgaccgg gtgggtccgc acctccgacc tggcgccgct
     6601 ccctgaccag gcgatggtcg gcgtgtcggc ggcggaggtc cgcgccgacc cggcgcgcta
     6661 catcgggcgg gtcgtcgaat ggcggctcca attcgtctcc gtgatggtcg ccgacgagct
     6721 gcggcccgag atcccggcgg ggcggagcta cctcctgacc cgcggcccgc ttcccgaatc
     6781 gggattcgtc tacgtggtcc tcccgcccga ccaggtcgag cgcttccgca cgctcgaacc
     6841 gctcaccgag ctgacgctcc gggtgcaggt ccgcgccgcg agcaccaagt tcctgccgaa
     6901 cccggtggtc gacctgctgc aggtcgtcac gggcaggtag ccgccaggag gtcccgtgca
     6961 cgagcagctc aaggaacgga tcgcccgccg gctggagtcg ctggccgacg agcgcggcta
     7021 ccaggtgctc gactacatcg agttcctcga gagcaagtac ggcgaacgct ccacgccgac
     7081 caacttcttc gcccggctcg ccgagcgggt cgaggacacg ctccgcgccg gccgcatccc
     7141 ggtgaaggcc atcggcggga cgctcaccgc catggacgcg gccggcaagg tgatgaaggg
     7201 cgtcgtggcc gccggcaagg cggtcgtcga cgaggcggtg agcgcggcga acgctccggg
     7261 cgcgatcggc ccgccgaagg atccgcccaa gtaacgggtg acgcgtcggc gggggacgag
     7321 cgggggtcga gaccgctcac accccactcg ccgcccgaca cccgccactc gccacctctt
     7381 tcccggccac ccatgaccac cgactccccg tccaccgact tcatccgcgg catcggcccg
     7441 ctgcaggccg tctcgctcgt cgtcggttcg atgatcggca gcggcatctt catcgtctcc
     7501 gccgacatcg gccgccaggt gaacgcgtgg gggcccggcg gcctgatgct cgtctgggtc
     7561 ctgaccggcc tcatgacggt ggcgggcgcg ctctcgtatg cggagctcgc cgggatgatg
     7621 cccaaggcgg ggggccagta tgtcttcctc cgggaagggc tcaaccccct cgccgggttc
     7681 ctttacgggt ggaccctctt cgccgtcatc cagaccggta cgatcgccgc cgtggcggtg
     7741 gcgttcgcca agtacctcgg cgtcttcttc ccggccctga ccgaccacct cttcctgccg
     7801 ctcgggagct tcgcgctccc ggccgggctc ggcggtcagt cgatcgcgct cggactctcg
     7861 cccgagcggg tcgtggccat cctgagcgtc ctgttcctca cctgggtcaa cgtccgcggc
     7921 gtcgggctcg gcgccggcat ccagaccctc ctcaccatcg tgaagaccgt ggcgctgggc
     7981 ggcctgatcc tcctcggcat caccatcttc cgccacgccg atgtctgggc cgccaacgtc
     8041 gcgcatttct ggggcaccgc gccctggagc ctcgcgatgc tgccggtgat cgccgccgcg
     8101 atggtgggct cgctcttctc gagcgacgcg tggaacaacg tcaccttcgc cgccgccgag
     8161 gtccgggagc cggcgcggaa cctgccgcgg gccctggccc tcggcaccgg gctggtgacg
     8221 ctgctgtacc tcctggccaa cgtggcgtac ctccacgtcc ttcccttcgc cggcgacccg
     8281 gcgggggccg atgccctcgc gcgcgggatg cagcacgccg cctccgaccg gatcggggcc
     8341 gcggcggccg aggtgatgct cgggagcgcc ggcgcgctcg tgatggccgg cctgatcgtg
     8401 atctcgacct tcggctgcaa cgccgggctc atcctttcgg ggccccggct ctactgggcc
     8461 atggcgcagg acaagctctt ctttgccccg gcggccgcgc tccatccggt gcatcgcacc
     8521 ccgacgttcg ggctctgggc ccaggcggtg tggacctcgc tcctctgcct cacggggacg
     8581 tacaaccagc tcctcgactt cgtcatcccg gccgtcctgg tcttctactt cctcaccatc
     8641 atcgccctct tccggctccg cgccacgcgt cccgacctgc cgcggccggt caaggcgttc
     8701 ggctatccgg tggtgccggg gctctacctc gtggcgctcg cgttcgtcac cggagctcag
     8761 gtcctggcgc ggccgctctt tgccgccacc gggctcatca tcgtggccat cggggtgccg
     8821 gtgtacatgt ggtggcatcg gggcgcggcg acgacgaggc gtgattcgtg agctgtgagt
     8881 tgcgagtcgt aagacgtgac cgggggcttc gctgcgtggc ggaccccctt tcgcaacggt
     8941 aggaagtcgg taccttcgcc gcgacccgcg acccgcgacc cgcgacccgc gacccgcgac
     9001 ccgcgccttg ccgccttccg gcaccccgac tatccttccg catggaacac ctgactcccc
     9061 acggcctgcc ggcgcttccg ggcgcactcg gcgggccttc gtgcgtatag ccccggcccc
     9121 gaccgcatga gcgcccctcg cgtccgaccc acgcccgcac tgaccttcga cgacgtcctg
     9181 cttgccccgc gtcactcgct ggtgcacccc aaggacgtgg cgaccgccag ctggttcacc
     9241 cgcaagatcc cgctccagat ccccttcatc tccgccgcga tggacaccgt caccgaggcg
     9301 gagatggcca tcgcgatggc gcgtgcgggg gggatcggcg tcatccacaa gaacatgacc
     9361 gtcgagcgcc aggccgccga ggtggaccgg gtcaagcgct ccgagagcgg gatgatcctg
     9421 aacccgatca cgctgggtcc cgaccgtccg ctccgggagg cggcgcagct gatgagccgg
     9481 ttccggatca gcggggtccc gatcgtggac gggcaggggc gcctgatcgg catcgtgacc
     9541 aaccgtgacc tccagttcga gcggaacctc gaccagccga tctccgaggc gatgaccaag
     9601 gacaagctgg tgacggcgcc ggtcggcacc gggctcgatg cggcggaacg gatcctggcc
     9661 cggcaccgga tcgagaagct cccggtggtg gacgaggccg gggtgctcaa ggggctcatc
     9721 acggtcaagg acatcttcaa gcggaagcag caccccaagt ccaacaagga tcagcacggg
     9781 cgcctgcggg tggcggcggc ggtgggcggc tcgcccgcgg cggtggatcg cgcgcgcgcg
     9841 ctcctcgacg ccggggtcga cgcgctggtg gtcgattccg cgcacggcca cagcgagggg
     9901 gtcctcagga ccgtcgacgc actgcgcctg aagttccccg atgcgcagat cgtggcgggc
     9961 aacgtggcca ccgaggcggg tgcccgcgag ctcgtccggc gcggcgtgga cgcggtcaag
    10021 gtcggcgtgg ggcccggcag catctgcacc acccgggtgg tgaccggcgt cgggatgccg
    10081 caggtgaccg cggtgatgga cgccgtccgg ggcgcggggg aggtgccggt gatcgccgac
    10141 ggcgggatca agtactcggg cgacgcggtg aaggcgctgg cggccggggc ctgctcggtg
    10201 atgatgggcg ccatgctcgc cggcaccgag gagagccccg gggagtcggt gctggccgag
    10261 gggcgccgct acaagatgat ccgcgggatg gggagcatgt cggcgatgca ggacggctcc
    10321 gcggaccgct atttccagga gggggagatg gccccctcca agttcgtgcc cgaggggatc
    10381 gaaggccggg tcccctaccg gggcccggtg ggcgacgtgc tcttccagat ggtgggcggg
    10441 ctccgcagcg ggatgggata ctgcggggtc ggcgacatcg aggcgttgcg cacgcaggtc
    10501 gagttcgttc aaattaccac cgccgggctc cgtgagagcc acccgcacga cgtcaccatc
    10561 acccgggaag ccccgaacta cagcgcgtga tccgggcatc cgccggatcc cggcgggctc
    10621 cccggtcacg tgatcgactc cccccaggtc gaggtaccgc atgagcgaac ccgaagcgaa
    10681 gggcgcgccc ctccccgcgc atggcgccgg catctggcgc cgcaagagca tcacggagct
    10741 ccaggtcgag gccgagaccg accacagcct caagcgcgcc ctcggcgcgg tcaacctgac
    10801 ggcgctcggc atcggcgcca tcatcggcac cggcatcttc gtgctcaccg gcaccgtggc
    10861 cgccatcaac tcgggccccg cggtggtgct gtcgttcgtc ctggccgggg tcgcgtcgat
    10921 cttcgcggcg ctctgctact ccgagttcgc gtcgctcgtc ccgatggccg ggagcgccta
    10981 cacctacggc tacgcgacgc tgggcgagct catcgcgtgg atcatcggct gggacctggt
    11041 gctcgaatac gcgctcggcg cggtgaccgt ggccatcggc tggtcggggt acgtcgtgtc
    11101 gttcctcgac ggcatcggca tccacatccc ggcggcgctc accgccgcac ggggccagat
    11161 cgtcacgctt cccgacggct ccaccgtcac ggccatcttc aacctgccgg cggtgctgat
    11221 catcgcggtc gtcaccacgc tcctggtggt gggcatcaag gagtcggcca cggtcaacaa
    11281 cgccatcgtc gtgatcaagg tggcggtggt gctgctcttc atcgtgctgg cggccaaggc
    11341 catcaatccc gccaactggc acccgttcat cccgcccaac accggcgtcc gggagcagtt
    11401 cgggtggacc ggcgtgatgg cgggtgccgg agtcgtgttc ttcgcctaca tcggcttcga
    11461 cgccgtgagc accgcggcgc aggaagccaa gaacccccag cgcgacatgc cgatcgccat
    11521 catcgggtcg ctgctcatct gcaccctgct ctacatcgtg gtctccggca tcgccaccgg
    11581 cgtgatgccc taccagcagc tcaacgtccc cgacccgatc gccgtcgtgg ccgaccgcgc
    11641 cgggctcggc tggatctcgg gcctcatcaa gctcggcgcc atcgccggcc tctcctcggt
    11701 catcctggtc atgctcctgg gccagtcgcg ggtgttctgg acgatgtcca aggacgggct
    11761 cctcccgccc ttcgtggcca aggtgcaccc gaagttccac acgccgtgga tcacgtcgat
    11821 cgtcaccggc gtcggcgtcg ccatcttctc ggcgatcctc accgtccgtg aggcggggag
    11881 cctggtgtcg atcggcaccc tgctggcgtt cgtgatcgtg tccatcggca tcctggtgct
    11941 ccgggtccgc gagcccggcc tcccacgccc gttcaaggcg ccggtggtct ggttcaccgc
    12001 gccgatgggc gcgctctcgg ccctctacct gatgatctcc ctggagcggc gcacctggga
    12061 gcggctcatc atctggttcg tcctcggcat gttcgtctac atcctgtaca gccgacacca
    12121 ctcgaagctc cgcagccgct cctgagcacg tcgggatgaa cgaccgcgcc gccgcaaggc
    12181 ggcgctttcg ttccatcccg caccgtgaat ccccgggtac ccttgggccg tctatgccgg
    12241 ggcgccgtgg ttgacacccg ccccggaccg gcctaaactg gcgcgccgct ttccccggcg
    12301 gcgtctccca cgataccgag gaccgtcatg caattgctcc gtcggaagag cgtcaccgcg
    12361 ctccaggccg aggccgaggc cgaccacagc ctgaagcggg tgctgtcggc cacccagctc
    12421 acgatgctcg gcatcggggc catcatcggc accggcatct tcgtcctgac cggcaccgtc
    12481 gcggccgaga acgccggtcc cgcagtggtg ctctcattcg ggttggccgg tttcgcgtcg
    12541 atcttcgccg cgctctgtta ctccgaattc gcgagcctcg tgccgatggc c
//
LOCUS       DASUAP010000250         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1110583, whole genome shotgun sequence.
ACCESSION   DASUAP010000250 DASUAP010000000
VERSION     DASUAP010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110583"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1141)
                     /locus_tag="VFU45_06605"
     CDS             complement(170..1141)
                     /locus_tag="VFU45_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEU5358769.1"
                     /translation="MPTPKKKRAPKKSKKAAAARTRGLEPTKLTGGAPPKAVESLAAQ
                     ITKDGGEVLATYKDPLGGRWQIFASLPIGLVEPTPYQRDLSDAHVARLGDAIDKLDRF
                     LDPIIAVPADAGKYWSPNGYHRLGAMNQLGARTITALVVPEPDVAHRILALNTEKAHN
                     LKEKSLEVSRLAQALAEMDPGSTEKSHAEIFEEAPLITLGLCYQENGRFSGGAYNPIL
                     KRIDGFLARKLPEAIEIRKGRAAKLLELNEDVNEAVKKLKEKGLESPYLKAFVVARIN
                     HLRFVKAKDLDFDETIATIMKAAKRFNVANVKADQVARSGGAPIAEE"
     gene            1273..2121
                     /locus_tag="VFU45_06610"
     CDS             1273..2121
                     /locus_tag="VFU45_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU5358770.1"
                     /translation="MEQHREPSTEPGFVRRRAIRGYSLKEFAHPPHRRLPWHDHAEAS
                     ICFVVSGSYTERMDGRDWECVAGTMVFKPPHEGHGDRFGGRGGRCLLIEVDPGRLRAI
                     GAHTTITERPGLARTAALTAFGQRILRETSAADEASPLAIEGLILEILAEGSRAIDGG
                     AHGPAPAWLRQAHALIRDSFRDALTLSAVARAVGVHPSHLARAYRAHHGCSVGDQVRR
                     LRVEQAALELAATGAPPAEIALRAGFFDQSHFSRVFRRQTGLTPAAYRAASGVRTART
                     APQRPS"
     gene            2205..2801
                     /locus_tag="VFU45_06615"
     CDS             2205..2801
                     /locus_tag="VFU45_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358771.1"
                     /translation="MSGSTQVRTVILACTVLAGPALLPGAPGRPDARSTAATVPAPRD
                     GQHDFDFEFGHWKTHLRRLVHPLTGSTTWVEYEGTTVVRRVWGGRANLLELVADGPAG
                     HFEGLNLRLYDPGARQWSLNFSNSTVGTLSQPTIGEFRDGRGEFYDQEPLNGRAILVR
                     FVILPITRDSVRFEQSFSDNGGKTWELNWVATDTRITG"
     gene            complement(2791..>3252)
                     /locus_tag="VFU45_06620"
     CDS             complement(2791..>3252)
                     /locus_tag="VFU45_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5358772.1"
                     /translation="LSAPAPLNAALMLAAGVAWGVYSLRGHGTEDALAETAGNFARAV
                     PLVLLLALATLPALHARPSGIAWAALSGAVASGLGYAVWYQALRALTSVTAAVVQLAV
                     PVLAGIGGVALLGEQLTLRLAAAAVLVLGGIAMTILGRASTAPARASDVSP"
BASE COUNT          461 a         1107 c         1189 g          495 t
ORIGIN      
        1 gacggcgagg cagagggggg cgaagggagc tgcgccgagg tagcgaccga gcagcgcggt
       61 cagggccgcg gcaagcgcca cggcgatgat cgggaaggtg ggaagtccga ccccgcggag
      121 acccgtgggg ggtcgggcac gaacggtggt gtcggtcatg ggtccgtact cactcctccg
      181 cgatcggcgc cccgccgctc cgcgccacct ggtcggcctt cacgttggcg acgttgaacc
      241 gcttggccgc tttcatgatg gtggcgatcg tctcgtcgaa gtcgaggtcc tttgccttga
      301 cgaaacggag gtgattgatc cgcgccacga cgaacgcctt caggtacgga ctctcgagcc
      361 ccttctcctt gagcttcttc accgcctcgt tcacgtcctc gttgagctcg agcagcttgg
      421 cggcgcgccc cttccggatc tcgatggcct ccgggagctt cctggccagg aatccgtcga
      481 tccgcttgag gatcgggttg tacgccccgc ctgaaaagcg cccattctcc tggtagcaca
      541 agcccagcgt gatgagcggc gcctcctcga agatctcggc gtggctcttt tcggtcgatc
      601 ccggatccat ctccgcgagg gcctgtgcca ggcgtgagac ttcgagggac ttctccttga
      661 ggttgtgcgc cttctcggtg ttgagcgcca ggatccggtg ggcgacgtcg ggctccggca
      721 ccacgagcgc ggtgatcgtc cgggcgccga gctggttcat ggcaccgagg cggtggtagc
      781 cgttgggaga ccagtacttt ccggcgtcgg cggggacggc gatgatcggg tcgaggaagc
      841 ggtcgagctt gtcgatcgcg tcgccgaggc gggccacgtg ggcgtcggag aggtcgcgtt
      901 ggtagggggt cggctccacc agcccgatcg ggagcgacgc gaagatctgc caccgtccgc
      961 cgaggggatc cttgtacgtg gcaaggacct cgccgccgtc cttggtgatc tgcgcggcga
     1021 ggctctcgac cgccttgggc ggggcgccgc cggtgagctt ggtcggttcg aggccgcggg
     1081 tgcgggcggc ggcggccttc ttcgacttct tcggggcgcg cttcttcttg ggtgtgggca
     1141 tcgggatagt ctgcgcggca gcgacgccgg gggcaagggc gggccggtgc gcggcagatg
     1201 aaaccgcgcg ggggtaaggt cacgtcgtgg gccgcaatgc gcaccagacc gtcttggcac
     1261 ggggagggat cagtggaaca gcatcgggaa ccgtcgaccg aaccggggtt cgttcgccgg
     1321 cgcgccattc gggggtattc actgaaggag ttcgcgcacc cgccgcaccg ccgcctgccg
     1381 tggcacgacc acgctgaggc gagcatctgc ttcgtggtgt ccggatcgta cacggagcgg
     1441 atggatggcc gggactggga atgcgtcgcc gggacgatgg tcttcaagcc cccgcacgag
     1501 gggcacggcg accggttcgg cggccggggc gggcgctgcc tgctgatcga ggtcgatcca
     1561 ggccggctgc gcgcgatcgg ggcccacacg accatcacgg aacgcccggg cctcgcccgc
     1621 accgcggcgc tcacggcgtt cggccagcgg atcctccggg aaacgtccgc cgcggacgag
     1681 gcctcgcccc tggcgatcga aggcctgatc ctggagatcc tcgcggaagg gtcgcgggcc
     1741 atcgacggtg gggcccacgg tccggcgccg gcgtggctgc gacaggcgca cgcactgatc
     1801 cgcgattcgt tccgcgatgc gctgacgctc tcggccgtcg cacgcgccgt cggcgtgcac
     1861 ccgtcccacc tcgcgcgcgc gtaccgcgcg caccatggat gctcggtcgg ggaccaggtc
     1921 cggcggctcc gggtcgagca ggcggccttg gaactggcag cgaccggtgc gccgcccgcc
     1981 gagatcgcgc tgcgggcggg attcttcgac cagagccact tctcacgggt attccggcga
     2041 cagaccggcc tcaccccggc ggcgtatcgc gcggcctccg gggtgcgcac cgcccgtacc
     2101 gccccgcaac gcccgtccta gaccgctccg gcccgatgcc gtaacgtcgc tccggccgat
     2161 cgcgccggtc ggtccaagtg cacttcgccg accggagggt caacgtgagc ggttccaccc
     2221 aggttcgcac cgtcatcctt gcctgcactg tgctggccgg ccctgcactc cttcccgggg
     2281 cgcctgggcg cccggatgca cgatcgaccg ccgcgacggt cccggcgccg cgcgacgggc
     2341 aacacgattt cgacttcgag ttcgggcact ggaagaccca cctgcggcgc ctggtccatc
     2401 ccctcaccgg ctcgaccacg tgggtggagt acgagggcac gacggtggtg cggcgcgtgt
     2461 ggggtggccg ggccaacctg ctggagctcg tggcggacgg cccggccgga cacttcgagg
     2521 ggctcaacct gcgcctgtac gaccccggcg cgcgccagtg gagcctcaac ttctccaaca
     2581 gcacggtcgg cacgctgagc cagccgacga tcggtgaatt ccgggatgga cgtggcgaat
     2641 tctatgatca ggagccactc aatgggcggg ccatcctggt gcgcttcgtg atccttccca
     2701 tcaccaggga ctcggtccgg ttcgagcagt ccttctcgga caacggtggc aagacgtggg
     2761 agctcaactg ggtggccacc gacacgcgga tcacgggctg acatcggacg cacgggcggg
     2821 cgccgtgctg gcccgtccga ggatcgtcat cgcgattccc ccgaggacca gcacggccgc
     2881 ggcggcgagg cgcagtgtca gctgctcgcc gagaagtgcc acccccccga tccccgcaag
     2941 gaccggcacg gcgagctgga ccacggcggc ggtgaccgac gtgagcgccc ggagcgcctg
     3001 gtaccagacg gcgtagccga gccccgacgc cacggccccc gacagcgctg cccacgcgat
     3061 cccgctcggc cgcgcgtgga gcgccggcag cgtggcgagg gccagcagga gcaccagggg
     3121 gacggcgcgg gcgaagttgc cggccgtctc cgcgagcgcg tcctccgtcc cgtggccccg
     3181 gagcgaatag acaccccacg cgacgccggc cgcgagcatc agtgcggcgt tgaggggcgc
     3241 gggggcggaa ag
//
LOCUS       DASUAP010000251         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1301694, whole genome shotgun sequence.
ACCESSION   DASUAP010000251 DASUAP010000000
VERSION     DASUAP010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301694"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..959
                     /locus_tag="VFU45_06625"
     CDS             174..959
                     /locus_tag="VFU45_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358773.1"
                     /translation="MKQADQEVEMDGAEMMSRLSEYVDGTLSSAERAGVEAWLSGHPE
                     GRQAVAELERVKRRAAELSQRPVPDAVWAGVRAGIRGPGGKVAGRQDGRAVRSRVSFS
                     MVQLAAAAALLMVVGATGAWWALRGRRGPGQAIVTQPPVAMAHPTSATIDTAVAVPVT
                     AIPRAAARPERLVANTTRADRSYDHAISDLQRVVAENRGRLSPATVKVIGQSLARIDS
                     ALARAQRALEKDPRNAYLTGHVTEMRQRKLELLQQTASLVSAS"
     gene            979..1953
                     /locus_tag="VFU45_06630"
     CDS             979..1953
                     /locus_tag="VFU45_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5358774.1"
                     /translation="MLLLTITALALAGFQQGVPTPRPPRPPRPPHVVAPRVHVDVDVD
                     RDDGSDDGAAGGTTDTTIAVEKGVRLSVDNFSGRITVVAWDQDKVRVRTGADDESGVD
                     VSGGAVTLRVNGNSGRGGDQDDHDITLSVPAWMELDLSGNQVDIDVSGVQGAVQAETV
                     EGSVSLKGGDGNISLKSVDGDVTISGAKGRIELNTVEGEIRATDVAGDLDVQSVDGEI
                     TLDRVDSRSVTANSVDGAIRFSGPINAGGVYHLESHDGDVILQTDGPPDALMSISTFD
                     GEFESDYPVTVTGANSNRRMTFTLGAGKARVELETFDGKISLRRAPAR"
     gene            1977..2753
                     /locus_tag="VFU45_06635"
     CDS             1977..2753
                     /locus_tag="VFU45_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5358775.1"
                     /translation="MRGTTLLTLALVAGTAAPAAAQDFSWKGTLAAGKTLEIRGVIGG
                     IHATASSGDQIEVTATKRGRRSDPDDVKIQVVPWDGGVTICAVYPTPRRARHENECAP
                     HGGGSMTTDNNDVSVEFTVKVPAKVNFTGSTVNGAVSADGLGGDADLSTVNGDVSVDA
                     AGTVEASTVNGSIDATSGTNTWDGTLKFSTVNGGITLRVPDGLNADLRASTVNGRITT
                     DFPVTVTGSMSPRSLKGRIGTGGRDLDMETVNGSIEIRKK"
     gene            complement(2879..3247)
                     /locus_tag="VFU45_06640"
     CDS             complement(2879..3247)
                     /locus_tag="VFU45_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5358776.1"
                     /translation="MPPALYSAAVFVTDIERAITFYRDTLKLPLAKQGTFGAEFFAEG
                     TRLGVHPANHPNAQAMVGRETGLVLHVPGILDYCSDLMDAGVTFVSEPTRQSWGIMAL
                     IADPDGNRIALWEEIEDAKP"
     gene            complement(3296..>3680)
                     /locus_tag="VFU45_06645"
     CDS             complement(3296..>3680)
                     /locus_tag="VFU45_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HEU5358777.1"
                     /translation="CGSCTRCLDACPTGAFPEPGVVDATRCISYLTIEQDGPIPPELL
                     PRLEGWAFGCDVCNDVCPWNERFARETDVAAFADRRPLAGVTVEVLDRMSDGEFTDRF
                     GGTPLTRAGREGLARNLSAVGSRRE"
BASE COUNT          609 a         1200 c         1372 g          499 t
ORIGIN      
        1 ctcgaccacc tggccacgat cctttccacc accgcgcccg ccgcggtcgt gaagcagggc
       61 gacaagctgg tcggcatcgt aagccggcac gacctgctgc aggagttgat cgggaggtaa
      121 atgggtcgtg agtcatgggt cgtgagtcgt gggtcggtga aggtcggtgt ggtttgaagc
      181 aggcagacca ggaggtcgag atggacggcg ccgagatgat gtcgcgattg tcggaatacg
      241 tggacggcac gctctcgagc gccgagcgcg cgggggtgga ggcgtggctg tccgggcatc
      301 cggaagggcg tcaggcggtg gccgaactgg agcgggtcaa gcgccgggcg gcggagctgt
      361 cgcagcgtcc ggtgccggac gcggtatggg cgggagtgcg ggcggggatc cgcgggccgg
      421 gcgggaaggt cgccggccgg caggatggcc gggcggtccg gagccgggtc tcgttctcga
      481 tggtccaact tgcggcggct gctgcgctgc tgatggtggt cggcgcgacc ggcgcctggt
      541 gggcgctccg cggccgcagg ggcccggggc aggcgatcgt gacgcaacct ccggtggcga
      601 tggcccatcc tacttccgcg acgatcgaca ccgccgtcgc ggtgccggtc acggcgatcc
      661 cgcgggcggc ggcaaggccc gagcgactgg tggccaacac cacccgcgcc gaccggagct
      721 acgaccacgc catcagcgac ctgcagcggg tcgtggcgga gaaccggggg cgcctgagcc
      781 cggcgacggt gaaggtgatc gggcagagcc tggcccgcat cgacagcgcg ctggcgcggg
      841 cccagcgcgc gctggagaag gacccgcgga acgcctactt gaccggccat gtgaccgaga
      901 tgcgccagcg aaagctcgag ctgctccagc agaccgcgag cctcgtgtcg gcgagctgaa
      961 ccggatggag gaatcacgat gctgttgctg accatcaccg cgctcgcgct ggcgggcttc
     1021 cagcagggag tccccacgcc gcggccgccc cgccccccgc gcccgccgca cgtcgtggcg
     1081 cctcgagtgc acgtggatgt cgatgtcgac cgcgatgacg gctccgatga cggcgccgcc
     1141 ggcggcacca ccgacaccac gatcgcggtc gagaagggcg tgcgcctgtc ggtggacaac
     1201 ttctccgggc gcatcaccgt ggtcgcgtgg gaccaggaca aggtgcgcgt ccggaccggc
     1261 gccgacgacg agagcggcgt cgacgtgtcc gggggcgccg tgacgctccg ggtcaacggg
     1321 aacagcggcc gcgggggcga ccaggacgac cacgacatca cgctctcggt gccggcgtgg
     1381 atggagctcg acctgagcgg caaccaggtg gacatcgacg tgagcggggt gcagggcgcg
     1441 gtgcaggcgg agaccgtgga gggttcggtg tcgctcaagg ggggcgacgg caacatctcg
     1501 ctcaagtcgg tcgacgggga cgtcaccatc tcgggcgcca agggccggat cgagctcaat
     1561 accgtggagg gggagatccg cgccaccgac gtcgctggcg acctcgatgt ccagtcggtg
     1621 gatggcgaga tcacgctcga ccgggtcgac tcccgctcgg tgaccgccaa ctcggtggac
     1681 ggcgccatcc gcttttccgg gccgatcaac gcgggcgggg tgtatcacct ggagtcgcac
     1741 gacggcgatg tgatcctcca gaccgacggc cctcccgatg cgctgatgtc gatcagcacc
     1801 ttcgacggcg agttcgaatc cgactatccg gtgacggtga ccggcgccaa ctcgaaccgg
     1861 cggatgacct tcaccctcgg cgcagggaag gcccgggtgg agctcgagac gttcgacggc
     1921 aagatctcgc tccggcgcgc gccggcgcgc tgaccccaac cagacggagc tacacgatgc
     1981 gcggcacgac gctcttgacc ctggccctcg tggccgggac cgccgccccg gcggcggcac
     2041 aggacttcag ctggaaaggc acgctggcgg caggaaagac gctggagatc cggggcgtca
     2101 tcggcggcat ccacgccacc gcctcttccg gcgaccagat cgaggtgacc gccaccaagc
     2161 ggggccgccg gagcgacccg gatgacgtca agatccaggt ggtgccgtgg gatggcggcg
     2221 tcacgatctg cgcggtctac ccgacgcccc ggcgggcccg gcacgagaat gaatgcgcgc
     2281 cccacggcgg cggcagcatg accacggaca acaacgacgt gtcggtcgaa ttcacggtga
     2341 aggtgccggc caaggtcaac ttcaccggat ccacggtcaa cggcgcggtc tcggccgacg
     2401 gcctcggcgg cgacgccgac ctttcgaccg tcaacggcga cgtctcggtc gacgccgcgg
     2461 gcacggtgga ggcctcgacc gtgaacgggt cgatcgatgc caccagcggc accaacacct
     2521 gggacgggac gctcaagttc agcaccgtga acggcgggat cacgctgcgg gtgcccgacg
     2581 gattgaatgc cgacctgcgc gcctcgacgg tgaacggcag gatcacgacc gacttccccg
     2641 tcacggtgac gggttcgatg agcccgcgga gcctcaaggg gcggatcggg accggcggac
     2701 gcgacctcga catggagacc gtcaacgggt cgatcgagat caggaagaag taggcgggtg
     2761 ggcgcatagg cgcacagacg cacaaggcgc ataggggtcc tttgtccccg atgcgcctta
     2821 cgcgtccacc cgcccgtgcg cctatgcgtc cgtgcgcctt acgcgcccgg tccgccggct
     2881 acggcttggc gtcctcgatc tcctcccaca gcgcgatgcg gttgccgtcg ggatcggcga
     2941 tgagggccat gatgccccac gactgacggg tcggttcgga gacgaacgtc acgccggcgt
     3001 ccatcaggtc gctgcagtag tcgaggatcc cggggacgtg gagcacgagc cccgtctccc
     3061 ggccgaccat cgcctgggcg ttgggatggt tggccgggtg cacgccgagc cgggtgcctt
     3121 cggcaaagaa ctcggcgccg aaggtgccct gcttggcgag cggcagcttg agcgtgtcgc
     3181 ggtagaacgt gatggcgcgc tcgatgtcgg tgacgaagac ggcggcggag tagagggcgg
     3241 gaggcatcgt gcggtgaacg gtaagcagtg aacggtgaac gggaagtccc cggattcatt
     3301 ctcgccggga tccgacggcc gagaggttac gggccaggcc ttcccgcccc gcccgcgtga
     3361 gcggcgtccc gccgaagcga tccgtgaact ccccatccga catccgatcg agcacttcca
     3421 cggtgacgcc agcgagcgga cgccgatcgg cgaatgcggc cacgtcggtc tcccgcgcga
     3481 accgttcatt ccacggacag acgtcgttgc agacatcgca cccgaaggcc cagccctcga
     3541 gccgggggag gagctccggc gggatcggac cgtcctgctc gatggtcagg taggagatgc
     3601 agcgggtggc gtcgaccaca ccgggctcgg gaaaggcgcc ggtgggacag gcgtcgaggc
     3661 accgggtgca gcttccacag
//
LOCUS       DASUAP010000252         6645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_852224, whole genome shotgun sequence.
ACCESSION   DASUAP010000252 DASUAP010000000
VERSION     DASUAP010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852224"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2074
                     /locus_tag="VFU45_06650"
     CDS             <1..2074
                     /locus_tag="VFU45_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU5358778.1"
                     /translation="GDGACMGCGEKTAVHLIVSTIVAAMQPRVKRHVAHLNSLITDLD
                     AEARGLVAAGVDLGAAASAKGQVSVPVAATTRERVALLDRALVQLRDLKWRYESGPGG
                     RGRASLGMANSTGCSSVWGSTYPYNPYPFPWVNHLFQDSPSVAIGLFEAQMRKMADDV
                     AMVRRAERLREGTYDAAKDEPALAALTWQGFTDEEFALCPPILSMGGDGAMLDIGFQN
                     LSRLLASGKPIRVVVLDTQVYSNTGGQACTSGYTGQVADMSGFGKAQHGKAEVRKELA
                     LLAIAHRGVYVHQSSQAAASHLIGGVLRGLHKRRPVLINVYTPCPVEHGLADDQSQHA
                     ARLALESRAFPFLTFDPDAGPTYADQLSLEGNPSLEDPWPTYTLKHLDGAGAEQSLEL
                     PVTIADWAATEGRFKQHFTEVPAEKWHDGMVPFHEFLALPSDARDGKVPYVLALAKDR
                     TLRRLAVTREIVSLAEERQRFWSQLRQLAGLEVAPAVRDLVSKSLEAAFEARSAELRA
                     GYEAEMAALKASYPAQMARQLATALLDRAGGSAAVAELLASLPPSPNGASAPATAPAP
                     VVAAAPRAPAPAPVAVAAPAPAPAAAPTASSAPAAVAGDEEMVLEPWIDSARCTTCNE
                     CINLNKKMFAYNADKQAYIKDPHAGTFAQLVTAAEHCPVSIIHPGTPLDPREKDLPKW
                     TARAAKFN"
     gene            2085..3410
                     /gene="rsxC"
                     /locus_tag="VFU45_06655"
     CDS             2085..3410
                     /gene="rsxC"
                     /locus_tag="VFU45_06655"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEU5358779.1"
                     /translation="MTASGFRHGVHPPEEKGLTSRLPIRRMPFPDEIILPLRQHAGKP
                     AIPCVRVGDHVERGDPVALADGWVSVPIHASAAGTVAAIDWWPHPDGSMAQAVRIRVD
                     RFAAQVPRPRMVPHWEGLSTDQVIGAVQDAGVVGLGGAAFPTHVKLAPPKDHAVHTLI
                     INGAECEPYLTSDHRTMAEYPDRVLFGIRVMMHCLGVTRCVVGVERNKPDAIAALERA
                     RPADLDISILPLTVKYPQGAEKMLIHAVTGIEVPSGQLPVTVGVVVQNVGSVSAIAEV
                     FETGLPLIERIVTVSGRGLRRPANLIVPVGTKLRDLIAYCGGMTDEGVEVIIGGPMMG
                     QAQADLDAPVLKGTTGVVVLRREETRQEKVYPCIHCGRCLEACPVFLNPSLLGDFARV
                     GRYEDMAEHHLADCMLCGSCAYVCPSNIPLAQFFQAGKAALRRAAAGAA"
     gene            3407..4402
                     /locus_tag="VFU45_06660"
     CDS             3407..4402
                     /locus_tag="VFU45_06660"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HEU5358780.1"
                     /translation="MTASLVVTASPHVRSQDSTPVIMWNVVGSLAPVVAAAAWFFGVS
                     GLLVVAAAVVGAVATEHFLGRGGTLADGSAAITGLLLGLALPPGLPLWMACLGGAFGI
                     AFGKLVWGGLGQNVFNPALVGRAFLQAAFPVAITTWPPVGGPFWALKGDLFALPFTRP
                     ATDAVTSATPLGLLKFEGHATRLLDLVTGATGGSVGETAALAILAGGAFLAWKRYLNW
                     RAPVSILLTVAVVSALIHLVDPHRPDAPFMLFSGGLMLGAVYMATDLVTSPITNLGRW
                     VFGFGIGVLVVVIRIWGGLPEGVMYAILFMNALVPFINRATQPRIFGSRGAKVAA"
     gene            4399..5073
                     /locus_tag="VFU45_06665"
     CDS             4399..5073
                     /locus_tag="VFU45_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HEU5358781.1"
                     /translation="MTDVAPHCAVGAEPQVHGTSSPAWRLLVTLAVAGALSGWLVATV
                     YNITLPAVRRHAAERLDTAVREVLRAPVRWDTLYLVHDALTRTTPAGDLDEVPKAYVG
                     YDAAGKRLGVAITAAEPGFQELVTLMIGYDPATGTLIGFTVLDEKETPGLGDKIERDT
                     DFTRQFAGRTPPLTGVKGRAPATPGEVQTITGATISSRAVVRIIDHAVERWRPLLAAW
                     DRGGAT"
     gene            5070..5768
                     /gene="rsxE"
                     /locus_tag="VFU45_06670"
     CDS             5070..5768
                     /gene="rsxE"
                     /locus_tag="VFU45_06670"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HEU5358782.1"
                     /translation="MTTEATAVAEPPDVVVVEPDFVPGAPPPASVVADIWRGITRENP
                     VLVQFLGMCPTMAVTNTLANGFAMGLATLFVLVGSGLIVALFSRYIAHEVRITSYILI
                     IATFVTLADMILAATYPDISKALGPFMPLIVANCLLLGRAEAFAAKVGPVRAVADGLG
                     MGLGFTASLSLLSSIREVLGRGSILGHDLFGRRYEPWVFMAMPPGGFFTLGLALLGLA
                     LIIELRRRRAAEAA"
     gene            5765..6343
                     /locus_tag="VFU45_06675"
     CDS             5765..6343
                     /locus_tag="VFU45_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HEU5358783.1"
                     /translation="MSHLSWIFVSTLLVNNFTLVMFLGLCSFFGVTGKIETAWRLGLA
                     NTFVLLLTAVGAWTLNTYVLTRTPFLRTIAFIVVIASGVQVVEMAVKKYFPVLFRQLG
                     IYLPLITTNCAILGLALFQTARHYTLAEALVFAIGAGLGLTLALSLMASIRERIEVAE
                     VPVIARRMGLVLVVASCLSMAFMGFAGMGSAP"
     gene            6340..6489
                     /locus_tag="VFU45_06680"
     CDS             6340..6489
                     /locus_tag="VFU45_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358784.1"
                     /translation="MSTTAIGILLCAGLFALYGLVPHRGCTGHCAGCTGSCGRYADEG
                     DHDDR"
BASE COUNT          881 a         2487 c         2257 g         1020 t
ORIGIN      
        1 cggggacggc gcctgcatgg gatgcggcga gaagaccgcc gtccacctca tcgtgtcgac
       61 gatcgtcgcc gcgatgcagc cacgggtgaa gcggcacgtg gcgcacctca actcgctcat
      121 caccgacctc gacgcggagg cacgcggcct ggtcgcggcc ggcgtggacc tcggagctgc
      181 cgcgtcggcg aagggccagg tctcggtgcc ggtcgcggcg acgacgcggg agcgggtggc
      241 gctgctcgac cgcgccctcg tccagctgcg cgacctcaag tggcgctacg agtccggccc
      301 cggcggccgc ggccgcgcgt cgctcggcat ggccaacagc actggctgct cctcggtgtg
      361 gggcagcacc tatccgtaca acccataccc gttcccctgg gtcaatcacc tgttccagga
      421 ctcgccgtcg gtggccatcg ggctcttcga ggcgcagatg cggaagatgg ccgacgacgt
      481 ggccatggtc cgccgcgccg aacgcctccg cgaggggacg tacgatgccg ccaaggacga
      541 gcccgcgctc gcggcgctca cctggcaggg cttcaccgac gaggagttcg cgctctgccc
      601 gccgatcctc tcgatgggcg gcgacggcgc gatgctcgac atcggcttcc agaacctctc
      661 gcggctcctg gcctcgggta agcccatccg ggtggtggtg ctcgacaccc aggtgtactc
      721 caacaccggc gggcaggcgt gcacctcggg ctacaccggc caggtggccg acatgtccgg
      781 gttcgggaag gcgcagcacg gcaaggcgga ggtccggaag gaactcgcgc tcctggccat
      841 cgcgcaccgc ggcgtctacg tgcaccagag ctcgcaggcc gcggccagcc acctgatcgg
      901 cggcgtgctc cgggggctcc acaagcgccg ccccgtgctc atcaacgtct acacgccctg
      961 cccggtggag cacggcctgg ccgacgacca gtcgcagcac gcggcccggc tggcgctcga
     1021 gagccgcgcc ttcccgttcc tgaccttcga ccccgacgcg ggcccgacct acgccgatca
     1081 gctctcgctc gagggcaatc cctcgctcga ggacccctgg ccgacctaca ccctcaagca
     1141 cctcgacggc gccggcgcgg agcagtcgct cgagctcccg gtcaccatcg ccgactgggc
     1201 cgcgacggag ggacgcttca agcagcactt caccgaggtg ccggccgaga agtggcacga
     1261 cgggatggtg ccgttccacg agttcctggc gctcccgtcc gatgcgcgcg acgggaaggt
     1321 gccgtacgtc ctggcgctcg ccaaggaccg caccctccgc cgcctggcgg tgacgcggga
     1381 gatcgtgtcc ctggccgagg agcggcagcg cttctggtcg cagctccggc agctcgcggg
     1441 gctcgaggtg gcgccggcgg tgcgcgacct ggtgtcgaag tcgctcgaag cggcattcga
     1501 ggcccggagc gccgagctcc gcgccggcta tgaggcagag atggccgcgc tcaaggcgag
     1561 ctaccccgcc cagatggcgc gccagctcgc gaccgcgctc ctcgatcggg ccggcggctc
     1621 ggccgcggtg gccgagctcc tcgcctcgct cccgccgagc ccgaacggtg cgtccgcacc
     1681 ggcgacggcg cctgccccgg tggtcgccgc ggcgccgcgc gcgccggcac cggctcccgt
     1741 cgcggtcgcg gcgccggcac cagccccggc cgcggcaccg acagcgtcgt cggccccggc
     1801 ggcggtggcc ggcgatgagg agatggtgct cgagccgtgg atcgactcgg cccgctgcac
     1861 cacctgcaac gagtgcatca acctcaacaa gaagatgttc gcgtacaacg ccgacaagca
     1921 ggcgtacatc aaggacccgc acgccgggac ctttgcccag ctcgtcaccg cggcggagca
     1981 ctgcccggtg tcgatcatcc accccggcac gccgctcgac ccgagggaga aggacctccc
     2041 caagtggacc gcgcgtgccg cgaagttcaa ttgagcgccg gccgatgacc gcgtccggct
     2101 tccgtcacgg tgtccacccc ccggaagaga aggggctcac ctcccgcctt cccatccggc
     2161 ggatgccctt ccccgacgag atcatcctcc cgctgcgcca gcacgccgga aagcccgcca
     2221 tcccctgcgt cagggtgggg gaccacgtcg aacgcggcga cccggtggcc ctcgcggacg
     2281 ggtgggtctc ggtccccatc cacgcctccg cggccggcac ggtggcggcc atcgactggt
     2341 ggccccaccc cgacggctcg atggcgcagg cggtgcggat cagggtcgac cgctttgccg
     2401 cccaggtgcc gcgcccgcgg atggtgccgc actgggaggg gctctccacc gaccaggtga
     2461 tcggcgcggt ccaggacgcc ggcgtcgtgg ggctcggcgg cgccgccttt ccgacgcacg
     2521 tcaagctggc gccgccgaag gaccatgcgg tgcacacgct catcatcaac ggcgccgagt
     2581 gcgagcccta cctcacctcc gaccaccgca cgatggccga ataccccgac cgcgtgctct
     2641 tcgggatccg ggtgatgatg cactgcctcg gcgtcacccg gtgcgtggtg ggcgtggagc
     2701 gcaacaagcc ggacgccatc gccgcgctcg aacgggcccg cccggcggac ctcgacatca
     2761 gcatcctgcc gctcaccgtc aagtacccgc agggcgcgga aaagatgctg atccacgccg
     2821 tgaccgggat cgaggtgccc tcgggccagc tgccggtcac ggtcggcgtg gtggtgcaga
     2881 acgtgggctc ggtctcggcc atcgccgagg tgttcgagac cggcctcccg ctcatcgaac
     2941 ggatcgtgac cgtgtcggga cgcggactcc gccgccccgc caacctgatc gtgccggtgg
     3001 ggaccaagct ccgcgacctc atcgcgtact gcggcgggat gactgacgag ggcgtggaag
     3061 tcatcatcgg cggcccgatg atggggcagg cgcaggccga cctcgatgcg ccggtcctca
     3121 aggggaccac cggcgtcgtg gtgctccggc gggaggagac ccggcaggaa aaggtctatc
     3181 cctgcatcca ctgcggccgc tgcctcgagg cgtgcccggt gttcctcaat ccctcgctcc
     3241 tcggcgactt tgcccgggtg gggcggtacg aggacatggc ggagcaccac ctggccgact
     3301 gcatgctctg cgggtcgtgc gcctacgtgt gcccctccaa catcccgctg gcgcagttct
     3361 tccaggcggg gaaggccgcg ctccggcgtg ccgcggcggg ggccgcatga cggcatccct
     3421 cgtcgtcacc gcctcgccgc acgtgcggtc gcaggactcg acgccggtca tcatgtggaa
     3481 cgtggtcggc agcctcgcgc ccgtggtcgc ggcggcggca tggttcttcg gcgtgagcgg
     3541 actcctcgtc gtcgccgccg cggtcgtcgg ggccgtggcc accgagcact tcctcggccg
     3601 gggcggcacc ctggccgacg ggtccgcggc catcaccggg ctcctcctcg ggctcgcact
     3661 tccgcccggc ctcccgctct ggatggcgtg cctgggcggc gccttcggga tcgccttcgg
     3721 gaagctcgtc tggggcggcc tcggccagaa cgtgttcaat cccgcgctcg tgggccgcgc
     3781 cttcctccag gccgccttcc cggtggccat caccacgtgg ccgccggtgg gcggaccgtt
     3841 ctgggcgctc aagggcgatc tcttcgccct gcccttcacc cgtcccgcca ccgatgccgt
     3901 cacatccgcc acgccgctcg gcctcctcaa gttcgaggga cacgccacca ggctcctcga
     3961 cctcgtgacc ggcgccaccg gcggctccgt cggtgagacg gcggcgctcg cgatcctggc
     4021 ggggggcgcc ttcctggcct ggaagcgcta cctcaactgg cgggcgcccg tgagcatcct
     4081 cctcaccgtg gcggtggtga gcgcgctcat ccacctcgtc gacccccacc gccccgacgc
     4141 gccattcatg ctcttctccg gcggcctgat gctcggtgcc gtgtacatgg ccaccgacct
     4201 cgtcacctcg ccgatcacca atctcgggcg ctgggtgttc ggcttcggga tcggcgtgct
     4261 ggtggtggtg atccggatct ggggcgggct ccccgagggc gtgatgtacg ccatcctctt
     4321 catgaacgcc ctggtcccgt tcatcaaccg agccacgcag ccgcggatct tcggcagccg
     4381 cggcgcgaag gtggccgcat gaccgacgtc gccccgcact gcgccgtcgg cgccgagccg
     4441 caggtccacg gcacgagcag ccccgcctgg cggctcctcg tgacgctcgc cgtggcgggg
     4501 gccctctccg ggtggctcgt ggcgacggtg tacaacatca cgctcccggc ggtcaggcgg
     4561 cacgccgccg agcggctcga caccgcggtg cgggaggtgc tgcgggcgcc ggtccggtgg
     4621 gacacgctct acctcgtcca cgacgcgctc acccgcacca cccccgcggg cgacctggac
     4681 gaggtaccca aggcctacgt cggctacgac gcggcgggca agcgcctggg cgtcgcgatc
     4741 accgcggcgg aaccgggctt ccaggagctg gtgacgctga tgatcggcta cgaccccgcc
     4801 accggcaccc tgatcggctt caccgtgctc gacgagaagg agacgcccgg cctgggcgac
     4861 aagatcgagc gcgataccga cttcacccgc cagttcgcgg ggcgcacgcc gccgctcacc
     4921 ggcgtcaagg gacgcgcacc cgccacaccg ggcgaggtgc agaccatcac cggggccacg
     4981 atctcctcgc gcgcggtggt gcggatcatc gaccatgccg tggagcggtg gcgcccgctg
     5041 ctcgccgcgt gggaccgggg aggtgcaacg tgacgaccga agcgaccgcc gtggccgagc
     5101 cgcccgacgt cgtggttgtg gaacccgact tcgtgcccgg ggcgccgccc cctgcctccg
     5161 tcgtcgccga catctggcgc ggcatcacac gggagaatcc ggtgctggtg cagttcctcg
     5221 ggatgtgccc cacgatggcg gtcaccaaca cgctcgccaa cggtttcgcg atggggcttg
     5281 ccacgctctt cgtgctggtg ggttcggggc tcatcgtggc gctcttcagc cgctacatcg
     5341 cgcacgaggt gcggatcacc tcctacatcc tgatcatcgc caccttcgtg accctggccg
     5401 acatgatcct ggccgccact taccccgaca tcagcaaggc gctcggcccc ttcatgccgc
     5461 tcatcgtggc caactgcctc ctcctcggcc gggcggaggc gttcgccgcc aaggtgggac
     5521 cggtccgcgc cgtggccgac gggctcggga tggggctcgg cttcaccgcc tcgctctccc
     5581 tcctgagctc gatccgtgag gtcctcggtc gcggttccat cctcgggcac gacctcttcg
     5641 gccgtcggta cgagccgtgg gtgttcatgg cgatgccgcc cggcggcttc ttcaccctgg
     5701 gcctggccct cctcgggctg gcgttgatca tcgagctgcg ccgccggcgc gccgcggagg
     5761 ccgcatgagc cacctctcct ggatcttcgt ctcgaccctg ctggtcaaca acttcaccct
     5821 ggtgatgttc ctggggctct gctcgttctt cggcgtcacc ggcaagatcg agacggcgtg
     5881 gcgactggga ctcgccaaca cgttcgtgct cctcctcacc gccgtcgggg cgtggacgct
     5941 caacacgtat gtgctgaccc gcacgccgtt cctccgcacc atcgcgttca tcgtggtgat
     6001 cgcctccggg gtgcaggtgg tcgagatggc ggtcaagaag tacttcccgg tgctcttccg
     6061 ccagctcggc atttacctcc cgctcatcac caccaactgc gccatcttgg ggctcgcgct
     6121 cttccagacc gcgcgccact acacgctggc ggaggcgctg gtcttcgcca tcggcgcggg
     6181 gctcgggctc acgctcgcac tctcgctgat ggcgtcgatc cgcgagcgca tcgaggtggc
     6241 cgaggtcccc gtcatcgcgc gccggatggg gctcgtgctg gtggtggcct cctgcctgtc
     6301 gatggcgttc atgggattcg ccgggatggg gagcgcgcca tgagcacgac cgcgatcggc
     6361 atcctgctct gtgccggact cttcgccctc tacggcctgg tgccccaccg cggctgcacc
     6421 ggccactgcg ccggctgcac cggttcgtgc ggacgttatg ccgatgaagg agaccacgat
     6481 gaccgatgac ctcttccgcc gcccgacgcg ccgggagttt ctgaccgtgg gcacggggat
     6541 cttcctggtg ctctcgctcc cccgcgcgct ccggcgccgg ccgccggtgt cccggcgcac
     6601 cgtcccgttg atggggacga tcgccgagat ccaggtggcg caccc
//
LOCUS       DASUAP010000253         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_130109, whole genome shotgun sequence.
ACCESSION   DASUAP010000253 DASUAP010000000
VERSION     DASUAP010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130109"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..>2295
                     /locus_tag="VFU45_06685"
     CDS             226..>2295
                     /locus_tag="VFU45_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5358785.1"
                     /translation="METLLQDLRYAVRTLAKAPGFAAVAILTLAIGIGANTAIFSAVD
                     TVLLKPLPFPNGDRLVQVSTAGFQGARFAVSYPDVEDLRRLTDVFSGVGASTQQRYNL
                     TGAGDPLEVQAASVTADLFSVLSVPPEVGRLFTAGDDHSPVALISHGLWVTSFGSDPK
                     IVGKPIGLDGKSYTVVGVMPAGFHYPDDAIAVWTPIGGALEQNPGSETDRNARFFTAL
                     GRLAPGATVARAVADLKVLSARLNAEDSAASAGAPGGGRRTFGIAISGGAPGAPNGGQ
                     LARALDKTLEATPLHEVAIGDTGRPLFILFGAVALVLLIACVNAANLLLARATARRRE
                     MALRQALGAARGRLIRQLLTESVLLSLSAAVVGSMMALWGIRALVSLWPRALPGGGDI
                     SVDWRILAFTFGLALVTGLVFGLIPAWRASAPQIEETLREESAGTTGSRPRRLQGSLV
                     IAEITVALVLLVGAGLLVKSFIRLSNVNPGFATNDLLAARVRLTPTRYATGPAQAQFF
                     DQMLAALSAHPGIQSASIAGTLPWSGAFRMVMFNPRMIRPDYPDPFMALGSYTVSPDY
                     FAAFRIPIRRGRAFTADDRNGAPAVAVVNRSTVKALWPDQDPIGKQIILGRPGGPRGV
                     TVITVVGVIDDIRSAGLDATPRPEIYTPAAQESSLNQMWVVFRATNGKPLQMASAIRE
                     AVHQADPE"
BASE COUNT          340 a          798 c          771 g          386 t
ORIGIN      
        1 ctgatggcgg tggccgcgct cgcaacttat ctgccggccc ggcgggcgac ccggacggac
       61 ccgatcgtgg cgttgcgggc agagtgacgg ggatggggga ttcgagattg ggaattcggg
      121 ggctttcggg agccaccaat caccaatccc gaatccccaa tcccccgcta cacgaccttt
      181 gacgggagct gggcatctat ccagcaaccc gaaccaggac cattgatgga aacgctgctg
      241 caggacctcc ggtacgccgt gcggacactg gcgaaggctc cgggtttcgc cgcggtcgcc
      301 attctcacgc tggcgatcgg catcggcgcg aacacggcca tcttcagtgc cgtcgacacg
      361 gtgctgctca agccgttgcc ctttccgaac ggcgaccgcc tggtccaagt cagtacggcg
      421 ggattccaag gcgcccgttt cgcggtgtcc tacccggacg tggaagacct gcgccggctg
      481 accgatgtct tctcgggcgt gggcgcgtcc acccagcagc gctacaacct caccggagcc
      541 ggcgacccgc tcgaggtgca ggcggcgtcg gtcaccgcgg acctgttctc ggtgttgagc
      601 gtgcctcccg aggtgggccg gctcttcacg gccggcgatg accactcgcc cgtcgccctg
      661 atctctcatg ggctctgggt cacgagcttt ggatcggatc ctaagatcgt cggcaagccc
      721 atcgggctgg acgggaagag ttacaccgtc gtcggcgtga tgccggcggg attccactat
      781 cccgacgatg ccatcgcggt ctggaccccg atcggagggg cgctcgagca gaacccgggc
      841 tccgagaccg accggaacgc ccgcttcttc accgccctgg gccggctcgc tcccggcgcc
      901 acggtcgcac gtgccgtcgc cgacctcaag gtgttgagcg cgaggctcaa tgcagaggat
      961 tcggccgcga gtgccggcgc cccgggaggt ggacggcgga ccttcggtat cgcgatcagc
     1021 ggcggggccc ccggcgcccc gaacggcggg cagctggcaa gagccttgga caagaccctc
     1081 gaagcgacgc ccctgcacga ggtggccatc ggggacacgg ggcggcccct gttcatactc
     1141 ttcggcgccg tggcgttggt cctcctgatc gcctgcgtga acgcggcgaa cctgctgctg
     1201 gctcgagcca cggcccgccg acgtgagatg gcgctccgac aggccctggg cgcggcgcgg
     1261 gggcgtctga ttcgccagct gctcaccgag agcgtcctgc tctcactctc ggccgccgtg
     1321 gtcgggtcca tgatggcgct ctggggcatc cgcgcgttgg tgtccctctg gccgcgagcc
     1381 ctccccggcg gtggggacat ctcggtggat tggcgtattc tggcattcac cttcggcctc
     1441 gccctcgtca cggggctggt cttcggcctg atcccggcat ggcgcgcctc ggccccccag
     1501 atcgaggaga ccctgcggga ggaatcggca ggaactaccg gctcccgccc tcggcggctt
     1561 caaggcagct tggtgatcgc cgaaatcacg gttgccctcg tgctgctcgt gggcgcgggg
     1621 ctcctggtga agagcttcat ccggctcagc aacgtgaatc caggcttcgc caccaacgac
     1681 ctgctcgcgg cgcgcgtgcg cctcacgccc acccggtatg ccacgggtcc cgcgcaggcg
     1741 cagttcttcg accagatgct ggccgcgctc tcggcacacc cgggcattca gtcggcgagt
     1801 atcgctggta cgctgccgtg gtccggcgcg ttccgcatgg tcatgttcaa cccccggatg
     1861 atcaggccgg attacccgga tcccttcatg gccctcgggt cgtacaccgt gtcccccgac
     1921 tacttcgcgg cgttccgaat tccgatccgg cggggacggg cgttcaccgc ggacgaccgg
     1981 aacggtgcgc cggccgtggc ggtcgtgaat cgctccaccg tgaaagcgct ctggcccgat
     2041 caggatccga tcggcaagca gatcatcctg ggccgaccgg gcggtccccg gggcgtgacc
     2101 gtgatcacgg tcgtcggagt catcgacgac atccgctcgg cgggcctcga tgcgacgcct
     2161 cgccccgaga tctacacgcc cgcggcgcag gagagctcgc tcaaccagat gtgggtggtg
     2221 ttccgcgcca ccaacggcaa accattgcag atggcaagcg ccatccgcga ggcggtgcat
     2281 caggccgatc cggaa
//
LOCUS       DASUAP010000254         5888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_784429, whole genome shotgun sequence.
ACCESSION   DASUAP010000254 DASUAP010000000
VERSION     DASUAP010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784429"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFU45_06690"
     CDS             <1..721
                     /locus_tag="VFU45_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5358786.1"
                     /translation="TGSVDGRMVLVGNAEWMRASGVSIGAAEPVAERLAGEGATPVYV
                     AVGGSVLGVIGVSDPVRATSAPAVAGLRRGGTEVVMLTGDHPRAAERVAAAVGIARVV
                     AGVSPAGKRAEIERLQREGKVVAMVGDGLNDAPALAQADIGIAMGTGTDVAMASADIT
                     LMRGDLAGVAQAIRLSKATLRIIRQNLFWAFAYNVVCIPVAAGVLFPAFGFRLSPAIA
                     AAAMAVSSITVVSNSLRLRSA"
     gene            868..1212
                     /locus_tag="VFU45_06695"
     CDS             868..1212
                     /locus_tag="VFU45_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU5358787.1"
                     /translation="MDFDVLPCACGRGADDSERKAAAVDPEVKVALGNRLRRIEGQVR
                     GLQKMVEEERYCADVLTQVASVQEALRSVGKTLLGNHLRHCATQAITSEDPGKRDAMY
                     DELVELFYRNAK"
     gene            complement(1444..2979)
                     /locus_tag="VFU45_06700"
     CDS             complement(1444..2979)
                     /locus_tag="VFU45_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5358788.1"
                     /translation="MNPVRRQEPVALSTAYRWYALALLTALNLLNYLDRNVIFALFEP
                     IKRDLSLTDAQLGWLGSAYILVFSVAALPFGVLSDLRSRRAVIAWGVSLWSAFTVLAG
                     LVRGFGSLFVTRAAVGIGEAAFGPASQSLVADYFSGKRRAFAMGILSAGLSLGGVLGL
                     WLGGKLEVVYGWRVTFMAVGLPGFALAVLAVRLVDPTRVLTPVRVTHLLRELRVSVDT
                     LIEQFNPLLVTAAFGAIIALWADLGFGAGSGTITMILAISAGLGFALNLQRWVRQIRH
                     DQIDETPFGGGVSEVFRDLVRATRLVFRTPTLVYVFLSGALVSFGLNGLVGWGTSFAS
                     RELGWSAAESAAHLGEWGLISGTAGTLFGGVFADWLRKYTVAGRVWAGAIGLLIGGVL
                     AMWLVTIRDPQRFVTVFAVAFFFLTWYNGPLAATIFDVVAARVSATVVGAYLLFSHVA
                     GDAIAFPLVGALSDRFGIDRAMLILPVVTVIGGLALLPAVRTVGGDGVRMTGEFPVVV
                     VEE"
     gene            complement(2976..4121)
                     /gene="speY"
                     /locus_tag="VFU45_06705"
     CDS             complement(2976..4121)
                     /gene="speY"
                     /locus_tag="VFU45_06705"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEU5358789.1"
                     /translation="MTRPSAPYLRGQRIDPQGITGRETAADLIDSAFLAYNAGRLKEG
                     CKLFTERMLDPSVTVGMSLTGAMTPAGLGMSTLIPLMEAGFVDWIISTGANLYHDAHF
                     GLGLRLHRGRHDADDVELRENGVVRIYDIFFDYDVLLSTDAFVREVSARPEFQRSMST
                     AEYHYLLGGYIREREKALKLTRRSLLAAAHELEVPIYTSSPGDSSIGMNVAEQALAGS
                     QLRFDVSADVNETAAIVLEAKRTGGKSGALIVGGGSPKNFLLQTEPQIQEVLGIDERG
                     HDYFLQMTDARPDTGGLSGATPAEAVSWGKIDPDMLPGTVVVYMDNTVALPLLTAYAL
                     NRHEKRPLKRLYARREAMMTRLRTEYQAALDFRDRRAGEAAESATKR"
     gene            complement(4118..4882)
                     /locus_tag="VFU45_06710"
     CDS             complement(4118..4882)
                     /locus_tag="VFU45_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU5358790.1"
                     /translation="MGRVSRRGPTTNGASTEDEAFLERGAPERPDALKHSDPWRVLRI
                     MGEFVDGFDALADVTDAVTIFGSARTSPDDPLYTTAIATARELARAGFPIITGGGPGI
                     MEAANRGASEGGALSIGCNIELPHEQGLNPYVDRGIEFRYFFVRKTMFLKYSTAFVVF
                     PGGFGTLDEMTEALTLIQTGKVKHFPVILVGSAYWHGLVEWLRGPVMNEGKIGPKDLD
                     LFHLTDDPAEVVRRIVAVREARAQRRRSAPPQESHP"
     gene            4956..5240
                     /locus_tag="VFU45_06715"
     CDS             4956..5240
                     /locus_tag="VFU45_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358791.1"
                     /translation="MDQGAHPGGSWFRLSLAVVPRALVSPSLAVALLGALWAFRRRNW
                     WRRPPFLPLPDGTYLRWRMYTAYGDESAVPPVRDLIRFARWRRELLRLGR"
     gene            5437..>5888
                     /locus_tag="VFU45_06720"
     CDS             5437..>5888
                     /locus_tag="VFU45_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5358792.1"
                     /translation="MTGPTPPHVVILGGGFAGLYAAKALRHAPVRITLVDRRNHHLFQ
                     PMLYQVATAALSPGDIASPIRSILRRQANVEVLLGEARRVDADAKRVELTDDSSIEYD
                     YCIVATGARHSYFGHDAWEAAAPGLKNLEDALAIRRRILLAFEAAEREP"
BASE COUNT          913 a         2076 c         2003 g          896 t
ORIGIN      
        1 caccggttcc gtggatgggc ggatggtgct ggtggggaac gcggaatgga tgcgcgcgtc
       61 gggagtgagt atcggcgccg ccgagcctgt cgctgaacgc ctggccgggg agggggccac
      121 accggtctat gtcgcggtcg gcggctcggt gctcggcgtc atcggtgtct ccgatcccgt
      181 ccgggccacc agcgccccgg cggtggccgg gctccgccgg ggcggcaccg aggtcgtgat
      241 gttgaccggc gatcatccga gggccgccga gcgggtcgcg gcggcggtcg ggatcgcgcg
      301 ggtggtggcg ggggtatcgc cggccgggaa gcgggccgag atcgagcggc tgcagcggga
      361 agggaaggtc gtcgcgatgg ttggggatgg cctgaacgat gcgccggcgc tcgcccaggc
      421 cgacatcggc atcgcgatgg gcaccggcac cgacgtggcg atggcctcgg ccgacatcac
      481 cctgatgcgg ggcgacctgg ccggcgtcgc acaggccatc cggctttcga aggcgacgct
      541 ccggatcatc cggcagaatc tcttctgggc cttcgcctat aacgtcgtct gcattcccgt
      601 ggcggcgggg gtgctcttcc cggcgttcgg gttccgcctg agtcccgcca tcgccgccgc
      661 agcgatggcg gtgagttcga tcacggtggt gtccaacagc ctgcgacttc ggagtgcgtg
      721 aagagagaag agtgaagagt gaacagtgaa gagtgaacgg gcgtgggagg cgtcaaactg
      781 agggacctcc gcttcgttcc ggatgacgcg tccggccacc cccgttcacc gctgaccgtt
      841 gactgttaac ccgaaaggaa gcgcgacatg gatttcgacg ttcttccctg cgcctgcggg
      901 cgcggggccg atgacagcga gcgcaaggcc gcggctgtcg atcccgaggt caaggtagcc
      961 ctcggcaacc ggctccggcg catcgaaggc caggtccggg gcctgcagaa gatggtcgag
     1021 gaggagcgct actgcgccga cgtcctgacc caggtggcgt cggtacagga ggcgctccgc
     1081 agcgtcggca agacgctcct cggcaaccac ctgcgccact gcgccaccca ggccatcaca
     1141 tcggaagacc cggggaagcg cgacgcgatg tacgatgagc tggtggagtt gttctatcgg
     1201 aacgcgaagt gacgcggagc tgatggtcgg gtaggcacac cgcgcagcag ttcgacccca
     1261 ggcctgcagg cgcctggggg cgggccgccg atctgcggga gcagggcagg gatccttcgg
     1321 tcgcttcgct ctctcaggat gactccgcct gttccctctt ccctgttccc tcttcccttt
     1381 tcccttttcc ctcttccctt ttcccgtttc ccttccccct tcccccttct tacttctcac
     1441 ttctcactcc tccaccacca ccaccgggaa ctctcccgtc atcctgacac cgtcgccacc
     1501 caccgtgcgc accgcgggca ggagcgccag gccgccgatg accgtcacca ccggcaggat
     1561 gagcatcgcc cggtcgatgc cgaagcggtc cgacagcgca cccaccagcg ggaaggcaat
     1621 cgcatccccc gccacgtggc tgaagaggag gtaggcgccg accaccgtgg cgctcacccg
     1681 cgccgccacc acgtcgaaga tcgtcgccgc cagcgggccg ttgtaccagg tcaggaagaa
     1741 gaacgccacc gcaaagacgg tcacgaaacg ctgcggatcc cggatcgtca ccagccacat
     1801 ggccagtacg ccgccgatca ggagcccgat ggcgccggcc catacccgtc ccgccacggt
     1861 gtacttccgg agccagtcgg caaagacccc gccgaagagc gtgcccgccg tgccggagat
     1921 gagtccccat tctcccaggt gcgccgccga ttcggccgcc gaccagccca gctcccggct
     1981 cgcgaaactc gtcccccacc cgaccaggcc attgagcccg aacgacacca gcgcgcctga
     2041 caggaatacg tacaccaggg tcggcgtgcg gaagacgagg cgtgtggccc gcacgaggtc
     2101 tcggaacacc tcggagacgc cgccaccgaa tggcgtttca tcgatctggt cgtgccggat
     2161 ctgccgcacc catcgctgga ggttgagggc gaatccgagc cctgccgata tcgcaagaat
     2221 catcgtgatg gtccccgagc cggcgccgaa gcccaggtcg gcccagagcg cgatgatggc
     2281 gccgaatgcg gcggtcacga ggagcggatt gaactgctcg atcagcgtgt ccacgctgac
     2341 gcggagttcc ctgaggaggt gggtcaccct gaccggcgtg agcacgcggg tcggatccac
     2401 cagccgcacc gcgagcaccg cgagcgcgaa ccccgggagc ccgacggcca tgaacgtgac
     2461 ccgccagccg tacaccacct cgagcttgcc gccgagccag agccccagca cgccgcccag
     2521 cgagaggccg gccgagagga tccccatcgc gaaggcgcgg cgcttgccgg agaagtagtc
     2581 ggccacgagc gactgcgacg cggggccgaa cgccgcctcc ccgatcccga ccgccgcccg
     2641 ggtcacgaag agcgacccga agccgcgcac cagccccgcg agcacggtga aggcgctcca
     2701 gagcgacacg ccccacgcga tgacggcccg gcgcgaacgc aggtcgctca gcaccccgaa
     2761 ggggagcgct gccacggaga agacgaggat gtaggcggag ccgagccagc cgagctgggc
     2821 gtcggtcagg gagaggtcgc gcttgatcgg ctcgaacagc gcgaagatga cgttgcggtc
     2881 gaggtagttg aggaggttga gggccgtgag cagcgccagg gcgtaccacc ggtacgcggt
     2941 cgacagcgcg accggctcct ggcgcctgac ggggttcaac gcttggtggc cgactccgcg
     3001 gcctcgccgg cccggcggtc gcggaagtcg agcgcggcct ggtactcggt gcggagccgg
     3061 gtcatcatcg cctcgcgccg cgcgtacagc cgcttgaggg ggcgcttctc gtgccggttg
     3121 agcgcgtacg ccgtgaggag cgggagcgcc acggtgttgt ccatatacac caccacggtg
     3181 cccggcagca tgtcggggtc gatcttcccc cagctcaccg cctcggcggg cgtggcaccg
     3241 gagaggccgc cggtgtcggg ccgcgcgtcg gtcatctgca ggaagtagtc gtggccccgc
     3301 tcgtcgatgc cgagcacttc ctggatctgc ggctcggtct ggagcaggaa gttcttgggg
     3361 cttccgcccc ccacgatgag ggcaccgctc ttcccgccgg tgcgcttggc ctcgaggacg
     3421 atggccgcgg tctcgttgac gtcggcgctc acgtcgaagc ggagctggct ccccgccagc
     3481 gcctgctctg ccacgttcat cccgatcgag ctgtcgcccg gcgagctggt gtagatcggc
     3541 acctcgagct cgtgcgccgc cgccagcagc gaccggcgcg tcagcttgag cgccttctcc
     3601 cgctcccgga tgtacccgcc cagcaggtag tggtactcgg cggtgctcat cgagcgctgg
     3661 aactcggggc gcgccgacac ctcgcgcacg aaggcgtcgg tggacaggag cacgtcgtag
     3721 tcgaagaaga tgtcgtagat ccggacgacg ccgttctcgc ggagctccac gtcgtcggcg
     3781 tcgtggcggc cgcgatggag ccgaaggccg agcccgaagt gcgcgtcgtg gtacaggttg
     3841 gccccggtgg agatgatcca gtcgacgaac cccgcctcca tcagcgggat cagcgtgctc
     3901 atcccgagcc ccgccggcgt catcgcaccc gtcaggctca tccccacggt caccgacggg
     3961 tcgagcatcc gctcggtgaa gagcttgcac ccttccttga gccggccggc gttgtacgcc
     4021 aggaacgccg aatcgatcag gtcggccgcg gtctcccggc cggtgatccc ctgcgggtcg
     4081 atccgctggc cgcggaggta gggggcggat ggtcgggtca cgggtggctc tcctgcggcg
     4141 gggcggagcg gcggcgctgg gcgcgcgcct cgcgcaccgc aacgatgcgt cgcaccacct
     4201 ccgccgggtc gtcggtcagg tgaaagaggt ccaggtcctt cgggccgatc ttcccttcgt
     4261 tcatgaccgg cccccggagc cactccacca gtccgtgcca gtatgcgctg ccgaccagga
     4321 tgaccgggaa gtgcttcacc ttgccggtct ggatcagcgt gagcgcctcg gtcatctcgt
     4381 ccagggtgcc aaatccgccg gggaagacca cgaaggcggt ggagtacttg aggaacatcg
     4441 tcttgcggac gaagaagtag cggaactcga tgccacggtc gacgtacggg ttgagccctt
     4501 gctcgtgcgg caactcgatg ttgcagccga tcgagagggc gcccccctct gacgcgcccc
     4561 ggttggccgc ctccatgatg ccgggcccgc cgccggtgat gatcgggaag ccggcccggg
     4621 ccagctcccg cgccgtcgcg atggcggtgg tgtagagcgg gtcatccggg gaggtccggg
     4681 ccgagccgaa gatggtcacc gcatccgtca cgtcggcgag cgcatcgaac ccgtccacga
     4741 actcccccat gatgcggagc acgcgccacg gatcggaatg cttgagcgcg tcgggccgct
     4801 cgggcgcccc ccgttcgagg aacgcctcgt cctcggtgga tgcaccgttg gtcgtcggcc
     4861 cgcgccggct cactcgcccc acgctacgct cctggctggc agggaatgct gccccggaca
     4921 ggccgggaat ataccggacc ctgttatata tgggcatgga ccagggcgcc catcccggtg
     4981 gttcgtggtt ccggctctcg ctggccgtgg tgccgcgggc gctcgtctcc ccctccctcg
     5041 ctgtcgcgct cctcggtgcc ctctgggcct tccgccggcg caactggtgg cgccggccgc
     5101 cgttcctccc cctccccgac ggtacctacc ttcgctggcg gatgtacaca gcctacggcg
     5161 acgagtcggc ggtgccgccc gtccgcgacc tgatccgctt tgcccgctgg cgccgcgagc
     5221 tcctgcggct aggtcgatga ccaccgtggg accacggagg gcacggagga tcacggagat
     5281 gctcggagga ccgcatgtga tgtggccttg cgggcggagc tcggtgcgca cgaccagacg
     5341 atacagttgt tctccgtgcc tctccgtgca gctccgtgcc cgagcttgcc ctgagcctgc
     5401 gaagggcggt cccacggttg atcgggacga cgccgcatga ccggaccgac cccaccgcac
     5461 gtcgtcatcc tcggcggcgg attcgcgggg ctctacgccg ccaaagccct ccgccacgcg
     5521 ccggtccgga tcaccctcgt cgaccgccgg aaccaccacc tcttccagcc gatgctctac
     5581 caggtggcca ccgccgccct gagccccggc gacatcgctt ccccgatccg ctcgatcctc
     5641 cgccgccagg ccaacgtcga agtcctcctc ggcgaggccc gccgcgtcga tgccgacgcc
     5701 aagcgcgtcg aactcaccga tgatagtagt attgagtacg actactgcat cgtggccacc
     5761 ggtgcgcgcc actcctactt cggccacgat gcgtgggaag cggcggcgcc gggactcaag
     5821 aacctcgaag acgccctcgc catccgccgc cgcatcctcc tcgccttcga ggccgccgag
     5881 cgcgaacc
//
LOCUS       DASUAP010000255         3692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_893084, whole genome shotgun sequence.
ACCESSION   DASUAP010000255 DASUAP010000000
VERSION     DASUAP010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893084"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFU45_06725"
     CDS             complement(<1..880)
                     /locus_tag="VFU45_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5358793.1"
                     /translation="MHQIRALVILALASAACAGNVGAPVIPRAAPVLPEPVEVSPTPV
                     PYGGRVNIDALLAQLTPRQKLAQLVVPWIAGNYEAFDDSALARAAGWVDSLQVGGIIV
                     STGSPLDVAAKLNYLQRRSPLPLLVAADLEGGTAGRFLGGTAFPTNMGVAATGRELDA
                     YEMGRITALEGRAVGIDLALAPVADVNNNPANPIINTRSFGEDASRVAILVRAEVRGM
                     EDHGLHATAKHFPGHGDTGTDSHIALPVLTSDWRRLDTLELVPFRSAVDAGVTAVMSA
                     HIALPGLDSGAIRPATL"
     gene            875..2119
                     /locus_tag="VFU45_06730"
     CDS             875..2119
                     /locus_tag="VFU45_06730"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEU5358794.1"
                     /translation="MHGKLVVGFVMVVTLGGCARSAPSLAPAPVAAAPGVDVLLTDSL
                     ALVRGRRVGLVTNQAGIDARGVGDVERLRAAGIDLVALFSPEHGFRGTAAPGAAVAST
                     RDSATGLPIYSLYGRTRVPTVAMLDRLDVILVDLQDAGARYFTYLATTVDVMRGAARA
                     GKPVVILDRPDPIGGAVQGNVLDTAYASDVGLLAVPMRPGMTLGELARLARSDLKLTA
                     DLSVIPVRGWRRDQALDATGLPFVPPSPNLPTLESLFHYPGLCLFEGTDLSVGRGSDA
                     PFEQVGAPWLDTARVLARIRGAKLPGVAFRGVTFTPVRPGDGKYADTLLRGIRLTVTD
                     RSTYDPTATAVHLLAAVRAVHPRQFTFADVQFDRLAGGRDLREALARGEGAGRIVRGW
                     AADRAAFLRRRAPFLLYPAAAH"
     gene            2270..2899
                     /locus_tag="VFU45_06735"
     CDS             2270..2899
                     /locus_tag="VFU45_06735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5358795.1"
                     /translation="MKLVPAAAERLYYGTVRPAVNVLVRSGIRPNTITSVGTAVVIAS
                     AVCYALGQIRWGGALLLLSGVADTLDGQVARAGNLATKFGAFYDSTLDRVGDGATFIG
                     IAVYLLSAPGVAWREVAVLACMLGVLSSLLVSYARARAEGIGVDCKVGIAQRAERIVG
                     LGVPSLLVGAGPSGVVLEVIVGALTVASIITVVQRFVYVYRQTEGARAG"
     gene            2940..>3692
                     /locus_tag="VFU45_06740"
     CDS             2940..>3692
                     /locus_tag="VFU45_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU5358796.1"
                     /translation="MSKAAKEVAPAKGKLGVMCVGLGAVASTFIAGVELVRTGRAQAI
                     GSLTQIGTIRLGKRTEGRTPLIKEFVPLAALDDLVFTAWDPVPDNAYESAVHCGVLER
                     HDHIEAIAPFLKSIVPMPAVFDQQFVKRLEGKNVKKGKTKRDLAEQLRQDIRDFKARH
                     KLDRLVMVWCASTEAFLQPGPAHQSLAAFEKAMDANDPTIAPSMLYAYAAIMEGVPYA
                     NGAPNLSCDFPALEQLAVDKGVPIGGKDFKTGQ"
BASE COUNT          475 a         1281 c         1385 g          551 t
ORIGIN      
        1 cgagcgtggc cggccggatg gccccgctgt cgagcccggg gagcgcgatg tgcgcgctca
       61 tcaccgccgt gacgcccgcg tccaccgccg agcggaacgg caccagttcg agggtgtcga
      121 ggcggcgcca atcggaggtg aggaccggca gcgcgatgtg gctgtcggtg ccggtgtcgc
      181 cgtggccggg gaaatgcttg gcggtggcgt ggaggccgtg gtcctccatc ccccggacct
      241 cggcgcggac gaggatcgcc acgcgagagg cgtcttcgcc gaaggagcgg gtgttgatga
      301 tcgggttggc gggattgttg ttgacgtcgg ccaccggcgc cagcgccagg tcgatcccga
      361 ccgcgcgtcc ttcgagggcc gtgatccggc ccatctcata cgcgtccagc tcccggcccg
      421 tggcggccac gcccatattg gtggggaagg cggtgccgcc gaggaagcgg ccggcggtgc
      481 cgccctcgag gtcggccgcc acgagcagcg ggagggggct ccggcgctgg aggtagttga
      541 gcttggccgc gacgtcgagc ggcgagccgg tggagacgat gatcccgccg acctggaggg
      601 agtcgaccca tcccgcggcg cgcgcgaggg ctgagtcgtc gaaggcctcg taattccccg
      661 cgatccacgg caccaccagc tgggcgagtt tctgccgggg ggtcagctgg gccagcagcg
      721 catcgatgtt cacccgtccc ccatacggga ccggcgtggg gctcacctcg accggctccg
      781 gcagcaccgg ggcggcccgc ggtatcaccg gggccccgac gttccccgcg caggcggcgg
      841 aggcgagggc caggatcacg agggcacgta tctgatgcac ggaaagctcg ttgttgggtt
      901 cgtgatggtc gtgacgctgg gcggatgcgc ccggtccgct ccctcgctcg cgccggcacc
      961 ggtggccgcc gccccggggg tcgacgtgct cctgaccgac agcctggcgc tcgtgcgcgg
     1021 gcggcgcgtg gggctggtca ccaaccaggc ggggatcgat gcccgcgggg tgggcgatgt
     1081 cgagcggctc cgcgccgcgg ggatcgacct cgtggcgctg ttcagccccg agcacgggtt
     1141 ccgcggcacc gccgcgccgg gcgccgctgt cgcttccacc cgcgactcgg cgaccggtct
     1201 cccaatctac agcctgtatg gccgcacgcg ggtgccgacc gtcgcgatgc tcgaccggct
     1261 cgacgtgatc ctggtcgacc tgcaggacgc cggcgcccgc tacttcacgt acctcgccac
     1321 cactgtcgac gtgatgcgcg gcgccgcgcg cgcggggaag ccggtggtca tcctcgaccg
     1381 cccggacccg atcggcgggg cggtgcaggg gaacgtcctc gacaccgcct acgcgtcgga
     1441 cgtgggcctg ctcgcggtgc cgatgcgccc cgggatgacg ctcggcgagc tggcgcgcct
     1501 cgcccggtcc gacttgaagc tcaccgccga cctgtcggtc atcccggtgc ggggctggcg
     1561 gcgcgaccag gcgctcgatg ccaccgggct gccgttcgtg ccgccgagtc ccaacctgcc
     1621 gacgctggag agcctgttcc attacccggg gctctgcctc ttcgagggga ccgacctgtc
     1681 ggtgggacgg ggcagcgacg cgccgttcga gcaggtcggg gcgccgtggc tcgacacggc
     1741 acgggtgctg gcacggatcc gcggggcaaa gctcccgggg gtcgcctttc ggggcgtcac
     1801 gttcaccccg gtgcggcccg gcgacgggaa gtacgcggac acgctcctca gggggatccg
     1861 gctcaccgtg accgaccgct cgacgtacga cccgacagcc acggcggtgc acctgctggc
     1921 tgccgtccgg gcggtgcacc cgcgccagtt cactttcgcc gacgtccaat tcgaccgcct
     1981 ggccgggggc cgggaccttc gggaagcgct ggcgcggggc gagggcgcgg ggcggatcgt
     2041 gcgggggtgg gcggccgacc gcgcggcgtt cctccggcgc cgtgcaccct tcctgctcta
     2101 ccccgcggcc gcgcattagc gccctctttg tgacggccgg aaaccgttgc gcggcgcggc
     2161 ctccggccgg tccggcacaa ttgccctaag ccgttctggc ggttatcctt tggcgctccg
     2221 ccgggccgct ggcggggcgc cattcccggt caccgaggat gcccgagcgt tgaagctcgt
     2281 tccagccgcc gccgaacgcc tctattatgg gaccgtccgt ccggcggtga acgtcctcgt
     2341 tcgctccggg atccggccca acaccatcac gtccgtgggg acggcggtgg tgatcgcctc
     2401 ggcggtctgt tacgcgctgg gccagatccg ctggggcggg gcgctgctcc tgctgagcgg
     2461 cgtggccgac acgctcgacg gccaggtggc gcgggcgggg aacctggcca ccaagttcgg
     2521 cgccttctac gattcgacgc tcgaccgggt gggtgacggc gccacgttca tcgggatcgc
     2581 ggtctatctc ctctcggccc ccggcgtcgc ctggcgcgag gtggcggtgc tcgcctgcat
     2641 gctcggcgtg ctgagctcgc tgctggtctc ctacgcgcgg gcccgggccg aggggatcgg
     2701 ggtcgactgc aaggtcggga tcgcgcagcg ggcggagcgg atcgtggggc tgggcgtgcc
     2761 ctcgctcctg gtcggcgcgg ggccctcggg ggtggtgctc gaggtcatcg tcggggcgct
     2821 cacggtcgcc tcgatcatca cggtggtgca gcgattcgtc tacgtctatc gccagaccga
     2881 gggagcgcgg gcggggtgag cctgcgggtc ccgtcggcgc attggaaagg acgcttgcag
     2941 tgagcaaggc agcgaaggag gtcgcccccg ccaaggggaa gttgggggtg atgtgcgtgg
     3001 ggctcggggc cgtcgcatcg acgttcatcg ccggggtgga gctggtgcgg accggccggg
     3061 cgcaggccat cggctccctc acccagatcg ggaccatccg cctggggaag cggaccgagg
     3121 ggcggacccc gctcatcaag gagttcgtgc cgctcgccgc gctcgacgac ctcgtcttca
     3181 cggcctggga cccggtgccg gacaacgcgt acgagtcggc cgtgcactgc ggcgtcctgg
     3241 agcgccacga ccacatcgag gccatcgcgc cgttcctgaa gtccatcgtg ccgatgccgg
     3301 ccgtcttcga ccagcagttc gtcaagcggc tggagggcaa gaacgtcaag aaggggaaga
     3361 ccaagcgcga cctggccgag cagctccggc aggacatccg cgacttcaag gcccggcaca
     3421 agctcgaccg cctcgtgatg gtctggtgtg cgtcgaccga ggcgttcctc cagcccgggc
     3481 cggcgcacca gagcctggcg gcgttcgaga aggcgatgga cgccaacgac ccgaccatcg
     3541 ccccgtccat gctctacgcg tacgccgcca tcatggaagg cgtgccgtat gccaacggcg
     3601 cccccaacct ctcgtgcgac ttcccggcgc tcgagcagct cgccgtggac aagggcgtgc
     3661 cgatcggcgg caaggacttc aagaccggcc ag
//
LOCUS       DASUAP010000256         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_143610, whole genome shotgun sequence.
ACCESSION   DASUAP010000256 DASUAP010000000
VERSION     DASUAP010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143610"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VFU45_06745"
     CDS             <1..1293
                     /locus_tag="VFU45_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358797.1"
                     /translation="ADRPDSATWAAAEQAVPLTPAERAAWRRIDSIARRPPDLTTMVR
                     RGITTSIRVLTDPAYFHYNRVEGAYVGLGDHWDPRPDLSAAARAGYATGSDEAEYALS
                     GQIRLSDAQRTWLGLEYHDQVVPRPTFVSGSYNPTVRALLFRVDPLDYYRERGTTLSL
                     STKLADFTDLELRYDDYAQSTLPVITDYSVFGSHRPQRPNIPVTAGQLRTLGGTLTFD
                     SRPLRRLGIRDERLPAAVWTRVSVGAELSEPLITGGDFRYERYSLQVQRRQRTLGLGI
                     TTITAAAGIGSGELPPQRYFTVDFGMKALTYQSGGFNTLSDTNYAGNRAAMIIVQHDF
                     DRLLFARSHLPLIDRLPWTLSVEGGIFWTDFVDHAAEPADLMVHTAPAGYGEAGFGLG
                     NLTPFLSPINLAAHFSWSLNSRYAADRRMHFGLSFFAR"
     gene            complement(1350..3026)
                     /locus_tag="VFU45_06750"
     CDS             complement(1350..3026)
                     /locus_tag="VFU45_06750"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5358798.1"
                     /translation="MRLSLAALSLMASFALPLAAQQPPPRRVFTHADTLHGSNTPERA
                     WWDAEFYDLHVAVHPADSSITGWNGITYRVLQPGSVMQLDLQLPLEMDSVVQDGRSLP
                     FRRDGNAFFVSLAAQQRAGERRSLRAYYHGGWHAGPADTGRRRYLGPFHWATDSGGRP
                     WIATSDEGPGASTWWPLKDYPADEPDSQRIAITVPDPMIDVSNGRLRSTTHHGDGTTT
                     YEWFVADPINSYDVAINASADYVPVRDTMAGAEGPLTVEFWALTPHRDKAAALMSEVK
                     GMLACYERWFGPYPWYRDGYKLIEVPYLGMEHQSGIAYGNEYLPGYLGRDLSGTGEGL
                     GWDYIVVHESAHEWWGNSVSAKDHADMWIHESFGMYAEALYLECQKDHAAGVRYLVGV
                     RKNIKNDEPIIGSYGVYDVPRSQDRYFKGANMLMTMRAVVADDAKWLATLRGLQRTFR
                     HQTVTASDIRDYMSRSTGVDLQKIFVQYQETTMVPTLEYKSDGKTLSYHWTDVIPGFD
                     MPVDVALGADSTAYTQLRPTAAWQTTPSTLGSSDTLRVDPEFYVVARRTP"
     gene            complement(3166..3375)
                     /locus_tag="VFU45_06755"
     CDS             complement(3166..3375)
                     /locus_tag="VFU45_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU5358799.1"
                     /translation="MARTTGTLKWFNDAKGFRIITPDGGAKDCFVHHSPIQGEGFKTL
                     AEGANVEFDMVQGQKGPAAEKVVTL"
BASE COUNT          561 a         1287 c         1271 g          618 t
ORIGIN      
        1 gccgaccgtc ccgacagcgc cacctgggcc gcggcagagc aggccgttcc attgaccccc
       61 gccgagcgtg cggcgtggcg acggatcgac tcgatcgcgc gccgccctcc cgacctcacc
      121 accatggtcc gccgcgggat caccacctcc atccgggtgc tgaccgatcc cgcgtacttc
      181 cactacaacc gcgtcgaggg cgcctacgtg gggctcggtg accactggga tccccgcccc
      241 gacctttcgg ccgccgcgcg ggccgggtat gccaccggga gcgatgaagc ggagtacgcg
      301 ctctcggggc agatccgcct ctcggacgcc cagcgcacct ggctcggcct cgagtatcac
      361 gaccaggtcg tgccccggcc cacgttcgtg tcaggttcct acaacccgac cgtgcgggcg
      421 ctcctcttcc gggtggaccc gctcgattac taccgggagc ggggcaccac gctgtcgctc
      481 agcaccaagt tggccgactt caccgacctg gaactccggt acgacgacta cgcgcagtcc
      541 accctgccgg tgatcaccga ctactcggta ttcggatccc accggccgca gcgccccaac
      601 atccccgtca cggcggggca gcttcgcacc ctcggcggca ccctcacctt cgattcccgg
      661 ccgctgcggc ggctgggcat ccgtgacgaa cggctccctg cggcggtctg gacgcgggtg
      721 agcgtgggcg cggagctctc tgagccgctg atcacggggg gcgacttccg gtacgagcgg
      781 tattcgctgc aggtccagcg gcggcagcgg acgctggggc tcgggatcac caccatcacc
      841 gccgcggcgg ggatcgggtc gggagagctg ccgccgcagc ggtacttcac cgtcgacttc
      901 gggatgaagg cgctgaccta ccagagcggg gggttcaaca ccctgagcga caccaactac
      961 gccggcaacc gcgcggcgat gatcatcgtg cagcacgact tcgaccggct gctctttgcc
     1021 cggagccacc tgccgctgat cgaccggctg ccgtggacgc tgagcgtgga gggggggatc
     1081 ttctggaccg acttcgtgga ccacgccgcc gagcccgccg acctgatggt ccacaccgcg
     1141 cccgcggggt acggggaggc gggcttcggg ctcggcaacc tgacgccgtt cctctcgccc
     1201 atcaacctgg cggcgcactt cagctggtcg ctcaattcgc gctacgccgc cgaccggcgg
     1261 atgcatttcg ggctgagctt ctttgcgagg taggggcggg cgccgccggc cctgagcccc
     1321 gcacccatgg ggaggccgca ggccgccccc tacggcgtcc gcctcgccac cacgtagaac
     1381 tccggatcca cccgcaacgt gtctgacgag ccgagcgtcg acggcgtcgt ctgccacgcc
     1441 gccgtcgggc ggagctgggt gtaggcggtg ctgtcggcgc ccaacgctac gtccaccggc
     1501 atgtcgaagc cggggatcac gtcggtccag tgatacgaca gcgtcttccc gtcgctcttg
     1561 tattcgagcg tcgggaccat cgtcgtctcc tggtactgga cgaagatctt ctggaggtcg
     1621 acgccggtgg agcggctcat gtagtcgcgg atgtcgctcg ccgtcacggt ctggtggcgg
     1681 aaagtgcgct ggagcccgcg gagcgtcgcg agccacttcg cgtcgtcggc gacgacggcg
     1741 cgcatcgtca tcagcatgtt ggcacccttg aagtaccggt cctgcgaccg gggcacgtca
     1801 tacacgccgt agctgccgat gatgggctcg tcgttcttga tgttcttccg cactccgacc
     1861 aggtaccgca cgccggcggc gtggtccttc tggcactcca ggtacagcgc ctcggcgtac
     1921 atcccgaagc tctcgtggat ccacatgtcg gcgtggtcct tggcgctcac gctgttgccc
     1981 caccactcgt gggcgctctc gtgcacgacg atgtagtccc accccagccc ttcgccggtg
     2041 ccggagaggt cgcggccgag gtagccgggg aggtactcgt tcccgtaggc gatgccgctc
     2101 tggtgctcca tcccgaggta ggggacctcg atcagcttgt agccgtcgcg ataccacgga
     2161 tagggcccga accagcgctc gtagcaggcg agcatcccct tgacctctga catcagggcg
     2221 gcggccttgt cccggtgcgg cgtcagcgcc cagaactcca cggtgagcgg gccttcggcg
     2281 ccggccatcg tgtcccggac gggcacgtag tccgccgagg cattgatggc cacgtcgtag
     2341 ctgttgatcg ggtcggccac gaaccactcg taggtggtgg tcccgtcgcc gtggtgggtg
     2401 gtggagcgga gccgcccgtt ggacacgtcg atcatcgggt cggggacggt gatggcgatc
     2461 cgctggctgt cgggctcgtc ggcgggatag tccttgagcg gccaccaggt gctggcgcca
     2521 ggtccctcat ccgacgtggc gatccacggc cggccgccgg agtcggtggc ccagtggaag
     2581 ggcccgaggt agcggcgacg gccggtgtcg gcggggccgg cgtgccagcc gccgtgatag
     2641 tacgcgcgca gggaccgccg ctcccccgcc cgctgctgcg cggccaggga aacgaagaac
     2701 gcgttgccat cgcgccggaa cgggagcgag cgcccgtcct gcaccacgct gtccatttcg
     2761 agcggcagct gcaggtcgag ctgcatcacc gacccgggct ggagcacccg gtacgtgatc
     2821 ccgttccacc cggtgatgct gctgtcggcc gggtgcaccg ccacgtgcag gtcatagaac
     2881 tcggcgtccc accaggcacg ctcgggcgta ttggagccgt gcagcgtgtc cgcgtgcgtg
     2941 aacacgcgcc gcggcggcgg ctgctgggcc gccaggggga gcgcgaagga cgccatgagc
     3001 gacagggcgg cgagcgagag gcgcatgccg gactcgggga tggccggggt gaaggcgggc
     3061 tgccggggag gggcccagca gacggggatc ccccccaata tgccacgaag ccggcccacg
     3121 gaggaggtcg aacggcttcg cgacgaccgc atgagcggcg ggtgcttaga gtgtgacgac
     3181 cttctcggcc gccgggccct tctgcccctg caccatgtcg aactcgacgt tggcgccttc
     3241 ggcgagggtc ttgaagccct caccctggat cggcgagtgg tgcacgaagc aatccttcgc
     3301 gccgccatcg ggggtgatga tgcggaagcc cttcgcgtcg ttgaaccact tcagggtacc
     3361 ggtggtacgt gccatgacct gctccttgtg tgtgtaatcg gagtccggtc aagccgtacg
     3421 ttcccgaccg cccggtcgtt cgtgatcgtg ccctcaccct gggcggcctt tcaacgcgaa
     3481 aggggccggc gccaggccag ccccggatcg atctctggaa gttcgtcgcg cgcatgcacg
     3541 acaagctgga tatgaggcag ggggatgtac ttcgaaacac tggggcccag tccgatctcg
     3601 gccgccaagc ctgtgtcccc cggattctcg acgcccgtcg caagtccccg cggactccaa
     3661 gtatcgtagt gctcaatgcg tttctatatg agaatgatac ttgccaaaaa gagaaggata
     3721 cattactatt tccgctg
//
LOCUS       DASUAP010000257         5446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_34172, whole genome shotgun sequence.
ACCESSION   DASUAP010000257 DASUAP010000000
VERSION     DASUAP010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34172"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(485..1297)
                     /locus_tag="VFU45_06760"
     CDS             complement(485..1297)
                     /locus_tag="VFU45_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358800.1"
                     /translation="MRVILGLGILAASAAPMSAQIPAEGITWSLYGLRAGSCIHFLAP
                     ADMIKDVAPSGFVPARADGTPAIHPALAQELKAEPKYDDWVPSRFCWFAFDSAATAGR
                     TVRAKDDTRRAQSRPVAVGYWAAAIHRKGSVDGASEWAALAIFSNQGQLTQSLVPARY
                     EVSSADFEISGIDGSTDTRYQVQFDKNLVGWDGHLGDPTAPERTADTLSVGQSYVRLL
                     LTAALGDSAYSPVGNLRVTGKGKMVAAMAASPIRMVSPIARSGVPLTWWFRK"
     gene            complement(1339..3078)
                     /locus_tag="VFU45_06765"
     CDS             complement(1339..3078)
                     /locus_tag="VFU45_06765"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5358801.1"
                     /translation="MKRSILLAAALAAAPASLPAQQAQVDSLAGAYWQAQLDRYPETA
                     TTLGLAGPHNGRLTDLRPAAFDAYDAHLRDLERAAATIDEMGLAPLGRVTLRTLQASL
                     ARDRSLRICRDELWSVDQLGGLQVSFPDLAAIQPTATAEERAFLLARWRRMGDEIDQQ
                     VQDLRAGLAAGYTAPRVDVERVIAQVERLVAMPVDSNPLLPASARPGAPHRDDRWAGQ
                     VRAEVGDVIIPSFRRYLAFLGDVYLPRARTAPGVSANPDGAACYRARIHAETTLDLGA
                     DSIHALGLADVARVRGEMLALARSRFGTSNLDSLLPALRSDTALAFRTRDEVLAAATA
                     AVDRMRPHLPELFGRLPTLPLVVEPMPGFEEADAPAAYYYPGAVDGARPGRYLGNTSA
                     PRTRPRYTVEALAYHEGIPGHHLQIALAQELPLPDFMRYGGGSTAFTEGWALYAERLA
                     DERGLYTDDLQRLGMLFFRSWRACRLVVDTGIHALGWSRDSAVAYLSANTGLSRADVE
                     NEVDRYIAHPAQALAYRLGEREILDLRAEAQDRLGSRFDLRTFHDAVLAAGAVPLPIL
                     KDNIDAWLSLQGR"
     gene            complement(3075..3653)
                     /locus_tag="VFU45_06770"
     CDS             complement(3075..3653)
                     /locus_tag="VFU45_06770"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5358802.1"
                     /translation="MNAIRPRLAVAGLLAFLPTVLVAQNPAAFVVSGPEAGTRAPFFV
                     LDAAGKAGRSDRPFDLSLQLGRVVVLAFYQRDFTPTATKELTAFADRFQELFGDSTTV
                     VGVGVDSLDLHQSFAQSLALPFALLADTGLHVARLYGSVRPDGYARYSVYVIGPDGRV
                     SWRDLRFDPAKDGSYDELRRAVAAAHAPPTRK"
     gene            complement(3700..4203)
                     /locus_tag="VFU45_06775"
     CDS             complement(3700..4203)
                     /locus_tag="VFU45_06775"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5358803.1"
                     /translation="MRTFAAGAVLLLALVGAPRAAAAQDEGIAVGAPAPAVTIADLDG
                     HPVDLGNDIGRRPVFLEFWATWCELCKALYPRMEAAAAKYGSKVAFYGINVTVNQTPA
                     RVRRYLAEHHPPFRTLYDADGVGVRAYQAPTTSFVVIVDRHGKVVYTGSGGDQDLDAA
                     LARVTAN"
     gene            complement(4200..4904)
                     /locus_tag="VFU45_06780"
     CDS             complement(4200..4904)
                     /locus_tag="VFU45_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5358804.1"
                     /translation="MTGDSIATLLRDHPLAALPALFGAGVLVSFTPCIYPMIPITAGV
                     LGGAARPGQGRGRAAALTSAYVLGLALVYASLGLVAGLTGSIFGAISSNPWAYFAIAN
                     LLLLAALALLDVFPVAAPARLVQWAARFGAGSVPGAFAMGATSGLVAAPCGAPAFGAV
                     LTFVAATQSAARGFVYLFVFSLGMSAVLVAVGLSAGALAALPRAGRWMVWVKRGSGVL
                     LLAMAEYYLVKMGSVM"
     gene            complement(4901..>5446)
                     /locus_tag="VFU45_06785"
     CDS             complement(4901..>5446)
                     /locus_tag="VFU45_06785"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU5358805.1"
                     /translation="LTEAGLGAKGIVASTAGLAILLQEGIGDTIRISLTPVPGGDRAE
                     EVRVGQQILQSLGLRSFVPQVTSCPGCGRTTSTFFQSMAQDIQGYLRDRMPAWKLEYP
                     GVEELRVAVMGCVVNGPGESKHADIGISLPGTFEDPVAPVYIDGKLKLTLRGDAIVAE
                     FLRLLEDYVAERYGAGQTAGR"
BASE COUNT          854 a         1816 c         2071 g          705 t
ORIGIN      
        1 gtccctgccc cgcggaacgg gggacgcgag gcgagtggcg ggtggagaat gggcgacgag
       61 tggtgggagc cggggggtgg tatccctgcc ctgccaggtg gcggaccgag tggcgggtca
      121 cgggtggggg aagggcgacg agtggcggga gcgggggggc tggtatccct gccccgcccg
      181 ggtaatggat cgggtggcgg gtcacaggcg gcgggtgggg aaagggcgac gagtggcgtg
      241 aggtacgcgg gggccctgcc ctgctggtcg gggggaaggt ggcccgggag acggtccgag
      301 tggggaccgt ccgccggtta cgggaagtca gcctgagcga agcgaaggat ctcaacctga
      361 tggtcgccac ggtcccgatg tcaccagcat cggtccgaga tccttcggtc gcgccggtcc
      421 ctcaggctga cgtcggcggc aggtggcggg cggctgatag ctgatagctg acggctgata
      481 gctgctattt ccggaaccac caggtgaggg ggacccccga tcgggcgatc gggctgacca
      541 tccggatggg gctcgcggcc atggctgcga ccatcttccc cttccctgtc acgcgcaggt
      601 tgccgaccgg cgaataggcc gagtctccga gcgcggcggt aaggaggagg cggacgtacg
      661 actggccgac gctcagcgtg tccgccgtgc gctcgggggc cgtcgggtcg cccagatggc
      721 cgtcccatcc gaccagattc ttgtcgaact gcacctggta acgggtatcg gtgcttccat
      781 cgatcccgga gatctcgaaa tcggccgagg agacttcgta gcgggctgga acgaggctct
      841 gggtcagctg tccttggttc gagaagatcg caagcgccgc ccactccgac gccccgtcga
      901 ccgagccctt ccggtggatc gctgcggccc agtaccccac cgcgacgggg cggctttggg
      961 cgcgacgggt gtcgtccttg gcccgcacgg tgcggccggc ggtcgcagcg gaatcgaagg
     1021 cgaaccagca gaaccgggag ggcacccaat cgtcatactt gggctccgcc ttgagctcct
     1081 gggccagcgc cgggtggatg gccggggtgc catcggcccg ggcggggacg aagcccgacg
     1141 gtgccacgtc cttgatcatg tctgccgggg ccaggaagtg gatgcacgac ccggccctga
     1201 ggccgtagag ggaccaggtg atgccctcgg ccgggatctg ggccgacatc ggggcggcgc
     1261 tggccgccag gatgcccagg ccgaggatca cacgcattga acgcacggga agccccctga
     1321 tatggatgcg gcggtaggtc agcggccctg caacgagagc caggcgtcga tgttgtcctt
     1381 caggatcggc aacggcactg cgccggcagc gaggacggca tcgtggaagg tccggaggtc
     1441 gaaccgggac cccaggcggt cctgcgcttc ggcgcgcagg tcgaggatct cccgctcacc
     1501 aaggcggtac gccagcgcct gcgcgggatg ggcaatgtag cggtcgacct cgttctcgac
     1561 atcggcgcgg gaaagcccgg tgttggcgct caagtatgcc accgccgaat cgcgcgacca
     1621 gccgagggcg tggatgccgg tgtccaccac gaggcggcag gcccgccagg agcggaagaa
     1681 gagcatcccg aggcgctgga ggtcgtccgt atagagccct cgctcgtccg ccaggcgctc
     1741 ggcatagagc gcccaccctt cggtgaaggc cgtcgatccg ccgccataac gcatgaagtc
     1801 ggggagcggc agctcctgcg ccagggcgat ctgcaggtgg tgacccggga tcccctcgtg
     1861 gtacgccagt gcttccaccg tgtaccgggg ccgggtgcgg ggcgcggacg tgttgcccag
     1921 gtaccgcccg ggacgggctc catcgaccgc gcccgggtag tagtacgccg cgggtgcatc
     1981 ggcctcttcg aaccccggca tcggctcgac cacgagcggc agcgtgggca ggcggccgaa
     2041 gagttcggga aggtgcgggc gcatccggtc gacggccgcc gtggccgcag cgagcacttc
     2101 gtcgcgcgtg cggaacgcca gggccgtatc gcttcgaagc gccgggagca gggagtcgag
     2161 gttcgaggtg ccgaaccgcg accgggcaag ggcgagcatc tcgccgcgga cccgggccac
     2221 gtcagcaagg ccgagggcgt ggatcgaatc cgccccgagg tcgagcgtgg tctcggcgtg
     2281 gatgcgggcc cggtagcagg cggcgccgtc agggttggcg gagacacccg gcgccgtgcg
     2341 ggcgcgcgga aggtacacgt cgcccaggaa cgccaggtaa cgtcggaatg acggaatgat
     2401 cacgtcgccc acctcggcgc ggacctgacc cgcccaccgg tcgtcccggt gcggcgcacc
     2461 gggccgggcc gaagccggca ggagcgggtt cgagtcgacc ggcatcgcca ccaggcgttc
     2521 gacctgcgca atgacccgtt ccacgtcgac gcggggggcc gtgtatcccg ccgcgagccc
     2581 ggcgcggagg tcctggacct gctggtcgat ctcgtcgccc atccggcgcc agcgcgccag
     2641 aaggaaggcg cgctcctcgg cagtggcggt cggctggatc gccgccaggt cgggaaagga
     2701 cacttgcagc ccgccgagct ggtcgaccga ccagagctcg tcgcggcaga tgcggaggct
     2761 ccggtcgcgg gcaagcgatg cctgcagggt ccgaagtgtc acccgcccga gcggggcgag
     2821 ccccatttcg tcaatggtgg cggcggcgcg ctcgaggtcg cgcaggtggg cgtcgtacgc
     2881 gtcgaaggcg gcggggcgga ggtcggtgag gcggccgttg tgcggtcctg caagtccgag
     2941 cgtggtggcg gtctccgggt accggtcaag ctgcgcctgc caatacgccc cggccagcga
     3001 atcgacctgc gcctgctggg ccgggaggga ggcgggggcc gccgccaggg cggccgcgag
     3061 gagaatggac cgcttcactt ccgtgtcggg ggcgcgtgcg ccgccgcgac tgcgcgtcgc
     3121 agctcgtcgt aactgccgtc cttggcgggg tcgaagcgca ggtcgcgcca gctgacccgg
     3181 ccatccgggc cgatcacgta cacggagtag cgggcgtagc cgtccggccg cacgctgccg
     3241 tagagccgcg ccacgtggag gcccgtgtcg gccagcagcg cgaacggcag ggccagcgac
     3301 tgggcaaagc tctggtgcag gtcgagcgag tcgaccccga cgccgaccac cgtggtgctg
     3361 tcgccgaaga gctcctggaa tcggtcggcg aaggcggtca gctccttcgt ggcggtcggg
     3421 gtgaaatcgc gctggtagaa ggcgagcacc accacccgtc cgagctgcag gctcaggtcg
     3481 aaggggcggt ccgaccggcc ggcctttccg gcggcgtcga gcacgaagaa cggggcgcgg
     3541 gtccccgctt ccggtccact caccacgaag gccgcggggt tctgggcgac gagcaccgtc
     3601 ggcaggaagg ccaggaggcc agcgacggcg agtcggggac ggatggcgtt catcgagcgc
     3661 tcgggaacgg gtggagaggg gcccgggcgt gcggccgggt cagttcgcgg tcacgcgggc
     3721 cagggcggca tcgagatcct ggtcgccgcc gctgccggtg tagaccacct tgccgtgccg
     3781 gtccacgatg acgacgaagg aggtcgtcgg ggcctggtag gcccgcactc cgacgccgtc
     3841 ggcgtcgtag agggtccgga acggcggatg gtgctccgca aggtaacggc gcacccgcgc
     3901 cggggtctgg ttgaccgtca cgttgatccc gtagaaggcc accttcgacc catacttggc
     3961 ggccgcggcc tccatccggg ggtacagcgc cttgcagagc tcgcaccagg tggcccagaa
     4021 ctcgaggaag accgggcgcc gtccgatgtc attcccaaga tccacgggat gtccgtcaag
     4081 atcggcgatc gtgaccgcgg gggccggcgc gcccacggcg atcccctcgt cctgcgccgc
     4141 agcggcgcgg ggcgcgccga cgagcgccag gagcagcacg gccccggcgg cgaaagtcct
     4201 cacatgacgc tccccatctt gaccaggtaa tactccgcca tcgcgagcaa taggacgccg
     4261 gaaccgcgct tgacccacac catccagcgg cccgcccggg gcagcgccgc aagcgcgccg
     4321 gccgagaggc cgaccgccac cagcacggcg gacatcccca gggagaacac gaacaggtac
     4381 acgaagcccc gcgccgcgct ctgggtggcc gcgacgaagg tgagcaccgc gccgaaggcc
     4441 ggcgcgccgc agggcgccgc gaccagaccc gacgtcgccc ccatcgcgaa ggccccgggg
     4501 accgatccgg cgccgaagcg cgccgcccac tgcaccagcc gggcgggcgc cgcgaccggg
     4561 aacacgtcga gcagcgcgag cgcggcgagg agcaggaggt tggcgatggc gaagtaggcc
     4621 caagggttcg agctgatggc gccgaagatg gagccggtaa gccccgccac gagcccgagg
     4681 gacgcgtaga ccagcgcgag ccccaggacg taggccgatg tgagcgccgc cgcgcgcccg
     4741 cgcccctgcc ccggccgcgc cgccccgccc aggacgccgg cggtgatcgg gatcatcgga
     4801 tagatgcagg gggtgaagct caccaggacg cccgcaccga acagcgcggg aagcgcggcc
     4861 agcggatggt cgcgcaggag ggtcgcgatc gagtcgcccg tcaccggccg gccgtctgtc
     4921 ccgccccgta gcgctcagcg acgtagtcct cgagcagtcg gaggaactcg gccacgatgg
     4981 cgtcgccgcg gagcgtgagc ttgagcttgc cgtcgatgta gaccggcgcc accgggtcct
     5041 cgaaggtgcc ggggagcgag atcccgatat cggcgtgctt cgattccccg ggaccgttga
     5101 ccacgcaccc catgaccgcc acgcggagct cctcgacgcc gggatactcg agcttccagg
     5161 cgggcatccg gtcgcggagg tagccctgga tgtcctgcgc catcgactgg aagaaagtgc
     5221 tggtggtccg gccgcagccg ggacagctcg tcacctgggg cacgaagctg cggagcccga
     5281 gcgactgcag gatctgctgg ccgacccgca cctcctcggc gcggtcgccc ccgggcaccg
     5341 gcgtcagcga gatgcggatg gtgtcaccga tcccctcctg caggaggatc gcaagccccg
     5401 cggtgctggc aacgatcccc ttggcgccaa gccccgcctc ggtcag
//
LOCUS       DASUAP010000258         7925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_784508, whole genome shotgun sequence.
ACCESSION   DASUAP010000258 DASUAP010000000
VERSION     DASUAP010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784508"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..2382
                     /locus_tag="VFU45_06790"
     CDS             385..2382
                     /locus_tag="VFU45_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5358806.1"
                     /translation="MFCRIAVVLLVAVPLRLAGQQPADTLHQHGDSLSRAPVRVQSIT
                     VTAAPMRRETATSGVRVSAATVRGTPASDAYDLVRQTAGIEVHDQGQGPGFASDASVR
                     GFSSDHSADIALWIDGVPNNEPFNGHAEGYNDWNLLLPEIIEGIDVIKGPVSPLYGNF
                     ALAGVVNVQTSDRMRGSEVLVSGGSYGRAQADLLTGFDHGSSGGLVAIRGLREVGWRP
                     NSGYRTGQVNGRIVTDLSPLLSLDASVALYGAAWDSPGFLTDSQFQARDFGAVADPTD
                     GGFKRHASERASLRYITDGGAVWRTTAYATQGRWQLFLTIPPEAGAGEGSGSQTEEED
                     TRYGFGLNSAMTWGLARGDLTIGLEGRLDHADFGRYFSTARIRDSADAVITGHQGSAG
                     LYLASNTDLTRHLRLSLGGRLDAVGTTSDAGTGPRSDTHAIVTPKAGLLYHLPRYADL
                     YATVSRGFRQADGVIEDPAVGFITAWAYETGIKADIKGVNADVAFFRMDVSAEQPFDP
                     VTLQPSIGGASRRQGVELGLRARAGRWVAIRGSWTFNDARYRSFITPAGDTLTGRTVY
                     NTSKYVGAIAATLGPESGGWSVTGSLNAVGPYAPFDEPGVELPAYGLVHLSAYLRRGP
                     IAFELGIRNVLDHVYPEVRAGGYVSPGLPRTIYTTVRYRWP"
     gene            2461..2534
                     /locus_tag="VFU45_06795"
     tRNA            2461..2534
                     /locus_tag="VFU45_06795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2495..2497,aa:Arg,seq:tct)
     gene            2563..2961
                     /locus_tag="VFU45_06800"
     CDS             2563..2961
                     /locus_tag="VFU45_06800"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEU5358807.1"
                     /translation="MTDPGLLPARGASHRSRGVAFVLALFFGVFGAHRFYVGRTSSGI
                     VQLLTLGGLGIWALIDCVLIGSGNFRDVDGRRLTRWDPQEQEPYGEDLPPEVVEEIDA
                     LHAHIAELTERLDFAERMLADPSRRPGAGR"
     gene            2975..4834
                     /locus_tag="VFU45_06805"
     CDS             2975..4834
                     /locus_tag="VFU45_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5358808.1"
                     /translation="MSSAPFCTACGATIPADAVECPVCHAPATPAEPVSTPAPANATR
                     PSAGAPEVDDDAPMQAELRAQLEEASLGEYDIIGELGRGGMATVYLAHEIALDRKVAI
                     KVMSPAVMLSPKMAERFKREARTAAALSHPNIVPIYAVRETDRLVYFVMKCLMGRPLD
                     SIIKEVGQLPVGMMQTIFSQVASALSYAHKRGVIHRDIKPANIMIDEDGAVVVTDFGI
                     AKAGETTGLTVTGSTIGTPAYMSPEQCGNKNVGPASDQYSLGTVAYEMLAGRVPFEGD
                     TAIGLMYAQFYHAPPPLEQFRPDVPAELAAAVMRMLEKDADHRWPSLEAAVKAVGVPQ
                     IEGIDDPIREEMARLGSSGSVPKLLALSHTPNSPLPRMSTTGPRSNPGLRSNPNAVTI
                     PFTASGPSRRLRRGFHLSGPAWAGIGGIGMVAVLYFLGVFRGRAVPPAVAAPPVAESA
                     ASATPPGAPKARAKPDPARARAERARNPESTATLAAAAAPPARREAAPPPLPADPRSD
                     TIVTRAAIDVVIKLFRQSIESRDLAKVAIAYPGLTVKQQQEFTDLFQKAQNLRMTLVV
                     EKLNKLTENSAEVTLRGSYQYTDAKSHRNKLDDYKKKATLAFAAGAWRLSEIH"
     gene            4927..6345
                     /locus_tag="VFU45_06810"
     CDS             4927..6345
                     /locus_tag="VFU45_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5358809.1"
                     /translation="MLGAAFTPRRADLGEMLRLAVPVVTVQVGLMFMGVVDTVMVGHL
                     SKEALAAVALGNVYVFASICIGMGTLMVMDPIVSQAVGAGDAVAVARGVQRGLVLALL
                     LTIPCTLVLLPGEAVLGWLHQPPEVVPLAARYTRATLAGMFPFFAFIVFRQTLQAMNR
                     MAPIVWVVVAGNLINLALNYGLIYGHWGLPALGVVGSGYATAVGRTALGAGLLALAWP
                     VLRPTLRPLRSDAFDPRPLLRMTRLGFPIAVTMELEYGAFAIVGLMMGTLGTLQMAGH
                     QIALNLASLTFMVPLGVSGAGAVLVGQAIGRGDADGMRRAAGAALVIGVGFMACTAVV
                     FTLTPGAFAALYTTDPGVYALAATLIPIAGLFQVFDGTQAVTVGILRGAGDTSAPMAI
                     NVLGFWLVGVPVSLLFGFTFHGGPVGLWWGFVAGLAAVALFLVLRLRVRFRQAVERVV
                     IDRFHTGEHPRPEPPMEVPSVM"
     gene            complement(6440..7687)
                     /gene="icd"
                     /locus_tag="VFU45_06815"
     CDS             complement(6440..7687)
                     /gene="icd"
                     /locus_tag="VFU45_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU5358810.1"
                     /translation="MANPTYTDLTAPVEGETITMQGGKLHVPDRPIIPFIEGDGTGPD
                     IWRASQLVFDAAVARAYGGKRKLAWFEVYAGEKAKQKFDNWLPDDTLRAIQHHLVAIK
                     GPLTTPVGGGFRSLNVTLRQKLDLYACVRPVRYFTGVPSPVKRPEDVNMVIFRENTED
                     IYAGIEWKAGSAEAKQLLTFLQGTLSVKNIRFPATSAFGIKPVSEEGTKRLVRSAIDY
                     ALAQKHESVTLVHKGNIMKFTEGGFRDWGYELAKQEYGAVEIDGGPWCRLPGGLVIKD
                     VIADAFLQQILTRPKEYGVVATLNLNGDYISDALAAQVGGIGIAPGANINYTSGHAVF
                     EATHGTAPKYAGQDKVNPGSVILSGEMMLRYMGWGEAADLIVRSLEKTISQKTVTYDF
                     ARLMEGAKEVKCSEFAKAMAGNM"
BASE COUNT         1076 a         2899 c         2693 g         1257 t
ORIGIN      
        1 gtgctcgatg cgccggcgat ggcgttgatg cacgcccagc acgccggcgc cgacatggcg
       61 tcgatgcccg ccgcgcacgc tcacgcccac caccgggcgt ccggcggcca gcaccccgcc
      121 cctgaccgtc cgtgctgcga cctctgcgcc accgcttgcg tctcccatgc cgtgggtggc
      181 gccggcccgc tcgtcgccgg gcccacggaa atttccccgt gggcacccgt ggcgcccgct
      241 gctgccgctc cggcacgcag cctccccgct cagctcctcc cccccgccgt cggtccccca
      301 gcgcgcgcct gatcccgccg tcccgatccg atcaggtcac agccgtccgg ccacagccgg
      361 tcgctccacg cgcttggggg tgtcatgttc tgtcgtatcg ccgtcgtgct gctcgtcgcc
      421 gtcccgctcc ggctcgcggg ccaacagccc gcggatacgc tgcaccagca cggcgactcg
      481 ctctcgcggg cgccggtccg ggtgcagtcg atcaccgtca ccgccgcgcc gatgcggcgc
      541 gagacggcca cgagcggcgt ccgcgtctcc gcagcgacgg tccggggcac gcccgccagc
      601 gacgcctacg acctcgtgcg ccagaccgcc ggcatcgagg tccacgatca ggggcagggc
      661 cccgggttcg cctccgatgc gtcggtccgc ggcttcagct ccgatcactc ggcggatatc
      721 gcgctctgga tcgacggggt gcccaacaac gagccgttca acggccacgc cgagggctac
      781 aacgactgga acctcctcct gccggagatc atcgagggga tcgacgtcat caaggggccc
      841 gtgagtccgc tctacggcaa cttcgcgctg gccggcgtgg tcaacgttca gaccagcgac
      901 cggatgcggg gaagtgaggt gctggtgtcg ggcgggtcgt acgggcgcgc gcaggcggac
      961 ctgctcaccg gcttcgacca cggatcgagc ggggggctcg tggcgatccg cggtctgcgg
     1021 gaggtcggct ggcgccccaa ctccggctat cggacggggc aggtgaacgg caggatcgtg
     1081 accgacctct cgccgctcct gtcgctcgac gcgagcgtgg ccctctacgg cgcggcgtgg
     1141 gactcgccgg gcttcctgac cgacagccag ttccaggccc gggatttcgg cgccgtcgcg
     1201 gatcccacgg acggcggctt caagcgccac gccagcgagc gggccagcct gcggtacatc
     1261 accgacgggg gcgcggtctg gcgcaccacg gcctacgcca cccaggggcg ctggcagctc
     1321 ttcctcacca tcccgcccga ggccggggcc ggcgagggca gcggcagcca gacggaggag
     1381 gaggacaccc gctacggctt cggcctcaac agcgcgatga cctgggggct cgcgcgcggc
     1441 gacctcacca tcggcctcga agggcggctc gatcacgcgg acttcggccg ctacttctcg
     1501 accgcccgca tccgcgactc cgccgatgcg gtgatcaccg gccaccaggg ctccgccggg
     1561 ctctatctgg catccaatac ggacctcacg cggcacttgc gactttccct tggcggccgg
     1621 ctcgacgcgg tgggcacgac gtccgatgcc ggcaccggtc cgcgttccga cacccacgcg
     1681 atcgtgacac cgaaggcggg actgctctac cacctcccgc gctacgccga tctgtacgcc
     1741 accgtgtccc gcgggttccg ccaggccgat ggcgtgatcg aggatccggc ggtgggcttc
     1801 atcaccgcgt gggcgtacga gacgggcatc aaggccgaca tcaagggcgt caatgccgac
     1861 gtcgcgttct tccggatgga cgtgagtgcc gagcagccat tcgatcccgt caccctgcag
     1921 cccagcatcg gcggtgccag ccggcgtcag ggggtggaac tcggcctgcg ggcgcgcgcc
     1981 ggacggtggg tggccatccg tgggtcctgg accttcaatg acgcccgcta tcgcagcttc
     2041 atcaccccgg cgggggacac cctgaccggg cggacggtct acaacacgtc gaaatacgtc
     2101 ggagccatcg ccgcgaccct tggccctgag tcgggcggat ggtcggtcac cgggagcctc
     2161 aacgcggtcg gcccctacgc cccgttcgac gagccgggcg tcgagctgcc ggcgtacggg
     2221 ctggtccacc tgagcgcgta tctccggcgc gggccgatcg cgttcgagct tggcatccgg
     2281 aatgtgctgg accacgtcta tcccgaggtc cgggccggcg gctatgtgtc ccccggcctg
     2341 ccccggacca tctacaccac ggtccggtat cgctggccgt aggccgggcc cgccgggggc
     2401 agggggtgag gttccgcgcc gcaccggcgc gggctacaat tcatccctta cgccgcgacc
     2461 gcgcccgtag ctcagctgga tagagcaaca gccttctaag ctgtgggtcg gaggttcgaa
     2521 tcctcccggg cgcatcgacc tccgccatcc catcaggatg ccatgaccga tccgggcctt
     2581 cttccagcac ggggcgcatc ccaccgctcg cggggggtgg cgttcgtgct ggcgctgttc
     2641 ttcggcgtgt tcggcgccca ccggttctac gtcggacgca cctcgagcgg catcgtccag
     2701 ttgctcacgc tcggcgggct cgggatctgg gcgctcatcg actgcgtgct gatcggcagc
     2761 ggcaacttcc gcgacgtcga cgggcgccgc ctcacgcgct gggatccgca ggagcaggag
     2821 ccctacggcg aggacctccc gcccgaggtc gtcgaggaga tcgacgccct gcacgcccat
     2881 atcgccgagc tgaccgaacg cctcgatttc gccgagcgga tgctcgccga tccgtcgcgc
     2941 cgtcccgggg cgggccggtg aggcaccgcc gcccgtgagc tccgccccgt tctgcaccgc
     3001 ctgcggtgcc acgatcccgg ccgacgcggt cgagtgcccg gtgtgccacg ccccggccac
     3061 cccggccgag ccggtgtcca cgccggcccc ggccaatgcc acccggccgt cggcgggcgc
     3121 gccggaggtc gacgacgacg caccgatgca ggccgagctc cgggcccagc tggaggaggc
     3181 gagcctcggc gagtacgaca tcatcggcga attgggccgc ggcgggatgg cgaccgtgta
     3241 cctggcccac gagatcgcgc tcgaccgcaa ggtggccatc aaggtgatgt cgccggcggt
     3301 gatgctctcg cccaagatgg ccgaacggtt caagcgcgag gcccgcaccg ccgcggcgct
     3361 cagtcacccg aacatcgtcc cgatctacgc cgtccgcgag accgaccggc tggtgtactt
     3421 cgtgatgaag tgcctgatgg gccggccgct cgacagcatc atcaaggagg tgggccagct
     3481 ccccgtcggg atgatgcaga ccatcttctc ccaggtggcg agcgcgctct cctacgccca
     3541 caagcgcggc gtgatccatc gcgacatcaa gcccgccaac atcatgatcg acgaggacgg
     3601 cgccgtcgtc gtcaccgact tcgggatcgc caaggccggg gagaccaccg gcctcaccgt
     3661 gaccggatcc accatcggca cgccggccta catgagcccg gagcagtgcg gcaacaagaa
     3721 tgtcggcccc gcgtccgatc agtactcgct cggcaccgtg gcctacgaga tgctcgccgg
     3781 gcgggtgccc ttcgagggcg ataccgccat cggcctgatg tacgcgcagt tctaccacgc
     3841 gccgccgccg ctcgagcagt tccgccccga tgtcccggcc gagctggcgg cggccgtgat
     3901 gcggatgctg gaaaaggacg ccgatcaccg gtggccgtcg ctcgaggccg cggtcaaggc
     3961 cgtcggagtg ccgcagatcg aggggatcga cgacccgatc cgggaagaga tggcccgcct
     4021 cgggagctcg ggctcggtgc cgaagctgct ggcgctctcg cacaccccca acagtccgct
     4081 cccgcggatg tcgacgaccg gcccgcgctc gaacccgggg ctccgttcca accccaacgc
     4141 cgtgacgatc ccgttcaccg cctccgggcc gagtcggcgg ttgcggcgcg gattccacct
     4201 ctccgggccg gcgtgggcgg ggatcggcgg gatcgggatg gtggcggtgc tctacttcct
     4261 gggcgtgttc cgcgggcgcg cggtgccccc ggccgtcgcg gcgccgccgg tcgcggagtc
     4321 ggccgcgtcg gcgacccctc cgggcgcccc caaggcccgt gccaagcccg atccggcgag
     4381 ggcccgggcc gagcgcgccc gcaacccgga gagcaccgcc acgctggccg cagccgccgc
     4441 cccgcccgcg cggagggaag cggcaccgcc gccgctcccg gccgacccgc ggagcgatac
     4501 catcgtgacc cgcgccgcca tcgacgtcgt gatcaagctg ttccgccagt ccatcgaatc
     4561 acgggacctg gccaaggtcg cgatcgccta tccgggcctc acggtcaagc agcagcagga
     4621 gttcacggac ctcttccaga aggcccagaa cctgcggatg accctggtgg tggaaaagct
     4681 caataagctc accgagaact cggccgaggt gacgctgcgt gggagctacc agtacaccga
     4741 cgcgaagagc caccgcaaca agctcgacga ctacaagaag aaggcgacgc tcgccttcgc
     4801 ggccggcgcg tggcgcctga gcgagatcca ctgaggaccg ggcgtccgat cgcagtctga
     4861 ccggggccgc ctcgcaaggg acggccccgt atcgcttcgc aggaccacga tccatcgggg
     4921 gcaagcgtgc tgggtgccgc attcacgccg cgccgggccg acctgggcga gatgctccgg
     4981 ctggcggtcc cggtcgtgac ggtgcaggtc gggctgatgt tcatgggcgt cgtcgacacc
     5041 gtgatggtcg ggcacctctc caaggaggcg ctcgccgcgg tggccctcgg caacgtgtac
     5101 gtctttgcct cgatctgcat cgggatgggc acgctgatgg tcatggaccc gatcgtttcg
     5161 caggcggtcg gggcgggcga tgccgtggcc gtcgcgcggg gcgtccagcg gggcctcgtc
     5221 ctggcgctcc tgctcaccat cccgtgcacc cttgtcctcc tcccgggcga agccgtcctc
     5281 ggctggctcc accagccgcc cgaagtggtc ccgctcgccg cgcgctatac ccgcgccacg
     5341 ctcgccggga tgttcccctt cttcgcgttc atcgtcttcc gccagacgct ccaggccatg
     5401 aaccggatgg cgccgatcgt ctgggtggtc gtcgcgggga acctcatcaa cctggcgctc
     5461 aactacgggc tcatctacgg ccactggggc ctgccggccc tcggcgtggt cgggtcaggc
     5521 tatgccaccg ccgtcgggcg cacggcgctc ggcgcggggc ttctggccct ggcgtggccg
     5581 gtgctccggc cgaccctccg gccgctgcgg tccgatgcct tcgacccgcg gccgctcctc
     5641 aggatgacac ggctcggctt tccgatcgcc gtcacgatgg aactggagta cggtgccttc
     5701 gccatcgtgg gcctgatgat ggggacgctc ggcaccctgc agatggcggg gcaccagatc
     5761 gccctcaacc tcgcgtccct cacgttcatg gtgccgctcg gtgtgtcagg cgcgggtgcc
     5821 gtgctggtgg gccaggccat cggccgcggc gatgccgacg ggatgcggcg cgccgccggc
     5881 gccgcgctcg tcatcggagt cgggttcatg gcctgcaccg ccgtcgtgtt caccctgact
     5941 ccgggcgcct tcgcggccct ctacaccacc gatcccgggg tgtacgcgct cgccgcgacg
     6001 ctcatcccga tcgccggcct cttccaggtc ttcgacggca cccaggcggt gaccgtcggg
     6061 atcctccgcg gggcggggga cacctccgcg ccgatggcca tcaacgtgct cgggttctgg
     6121 ctggtggggg tcccggtgag cctcctgttc gggttcacgt tccacggcgg accggtcggg
     6181 ctctggtggg gctttgtggc ggggctcgcg gccgtggcgc tcttcctcgt gctccggctc
     6241 cgggtccgct tccggcaggc ggtggagcgg gtcgtcatcg accgcttcca taccggcgaa
     6301 catccgcggc cggagccgcc gatggaggtg ccgtcggtga tgtgagcggg aacatgcatc
     6361 gagggcgccg acggtcatcc catcggcgcc ctcgatcgca tccatcgtcg gcggtaccgt
     6421 cgccgtgccg tctgaccgcc tacatgttcc ccgccatcgc cttggcgaac tccgagcact
     6481 tcacttcctt ggccccctcc atcagccggg caaagtcgta ggtcacggtc ttctgcgaga
     6541 tcgtcttctc cagggaccgg acgatgaggt ccgccgcctc gccccacccc atgtagcgga
     6601 gcatcatctc gccggacagg atgaccgaac cggggttcac cttgtcctgc cccgcgtact
     6661 tgggggccgt tccgtgggtg gcctcgaaga ccgcgtggcc gctcgtgtag ttgatgttgg
     6721 ccccgggggc gatcccgatc ccgcccacct gcgccgcgag cgcgtcggag atgtagtcgc
     6781 cgttcaggtt gagcgtcgcc acgacgccgt actccttggg gcgggtgagg atctgctgga
     6841 ggaaggcgtc ggcgatcacg tccttgatca cgagcccgcc aggcagccgg caccagggcc
     6901 cgccgtcgat ctcgacggcg ccgtactcct gtttggcgag ctcgtagccc cagtcacgga
     6961 agcccccttc cgtgaacttc atgatgttcc ccttgtggac cagcgtgacg ctctcgtgct
     7021 tctgcgccag ggcgtagtcg atggccgagc ggaccaggcg cttggtgccc tcctcggaca
     7081 ccggcttgat gccgaatgcg ctggtggccg ggaaccggat gttcttgacg ctcagggtgc
     7141 cctgcaggaa ggtgagcagc tgcttcgcct ccgcggagcc cgccttccat tcgatgccgg
     7201 cgtagatgtc ctcggtgttc tcccggaaga tcaccatgtt cacgtcctcc ggccgcttga
     7261 ccggcgacgg caccccggtg aagtagcgca ccgggcgcac gcaggcgtag aggtcgagct
     7321 tctggcgcag ggtcacgttg agcgaccgga acccgccgcc caccggcgtc gtgagcggcc
     7381 ccttgatggc caccagatgg tgctggatgg cgcgcagcgt gtcgtcgggg agccagttgt
     7441 cgaacttctg cttggccttc tcccccgcgt acacctcgaa ccaggcgagc ttccgctttc
     7501 ccccgtacgc cctggcgacc gcggcgtcga acaccagctg ggacgcccgc cagatgtccg
     7561 gtccggtgcc gtccccctcg atgaacggga tgatcggccg gtccggcacg tggagcttgc
     7621 cgccttgcat cgtgatggtc tcgccctcga cgggggccgt caggtcggtg tacgtggggt
     7681 tcgccatggt ggtcccggag tggtcgttcg tggggtgatg cgtggtgggc gagaagttaa
     7741 tcgccccgct agattctcgc ccgtgactcc gccgatctgg tcgcccactc ccgtcgacgt
     7801 cgcccgcgcc aacctgaccg ccttcatcgg cgcggtctcc gcgcgccatc ccgccgccgg
     7861 catctcggat tacgactcgt tgtaccgctg gtcgctgcgg gcgccgcagc atttctgggc
     7921 ggaag
//
LOCUS       DASUAP010000259         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_525012, whole genome shotgun sequence.
ACCESSION   DASUAP010000259 DASUAP010000000
VERSION     DASUAP010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525012"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFU45_06820"
     CDS             complement(<1..878)
                     /locus_tag="VFU45_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358811.1"
                     /translation="MSSRALLCAGVLALGAPAAARAQSVAQLQAIRIRLQREADSLQA
                     VRRAGPSVDTVRLAGGWTLLVSGAARPFAEREVPVALAEAGREFGGALSPSAGPIRVV
                     FDSLARPVLEVSFTGAGPGAFAGSDRLVGGAIGELVRSAARKAVWAEADATLRAWAGG
                     QALESPYATIAPAARIRLARDTGSVVTGCRHGDVAACERALSVSGGVSEVVRTSLFRY
                     VLERAGGTDRLPDLYAEPTRPVLDRLAALAGEPREVMVSDWRERLIGLGALTTPAALV
                     AALGALMITGLGIWGV"
     gene            complement(871..1506)
                     /locus_tag="VFU45_06825"
     CDS             complement(871..1506)
                     /locus_tag="VFU45_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358812.1"
                     /translation="MFRVILKQVWRDSRWMLLFLAACGFVIVRGALHLAVAPPPVQEP
                     EQILVEAQFWGLMMPVLAFTAATLLSIITWWPDRRGWWVYALTLPVRRERYALMRAGA
                     GGTVLAGLAAAFAIAVAAFAGRAVVPDGLHLYAGGLILRFALALLVSHALWTMFALVG
                     RPVAWAVVGMVVVAIGLELVGVHATGWILDALTGRWSPLHVLGGRWMLIDV"
     gene            complement(1506..2411)
                     /locus_tag="VFU45_06830"
     CDS             complement(1506..2411)
                     /locus_tag="VFU45_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358813.1"
                     /translation="MTDLAIETRGLGLRLGRNFALADVSLAVPVGAIYGFLGPNGSGK
                     TTTIRTLLGLYPGFSGAASLLGLPVPARLPEVLGRTGYVPERPHLYRTLTVAQSLEFH
                     SAFHRKWDPAWAAELAAHFGLKSDAEIGRLSKGETAKLMLLQALGQKPELLVLDEPTD
                     GLDPVVRRDVLAALLEYVERTHATVFISSHLVHELERICDWVGVMDQGRLVAQVRMEA
                     FKHGIKRIRVAAGSKVPAIGLVAVIGSETIAGADEWVVRDWTPDAAAGLGRAGVTVRE
                     VIDLDLEEAFVELLRSFRRAAGRAA"
     gene            complement(2408..2785)
                     /locus_tag="VFU45_06835"
     CDS             complement(2408..2785)
                     /locus_tag="VFU45_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5358814.1"
                     /translation="MFERLDPRSPTPLYAQIAERLKVAIASGDLAPGAALESVRALAA
                     RIRVNPATVVQAYRDLEAEGFVEMRQGSGTYVKAVSTERRGRQREAQARQLIRQMLAE
                     AARLGVQPAEIRAALHDELKETR"
BASE COUNT          487 a         1218 c         1073 g          398 t
ORIGIN      
        1 ctcacccccc agatgccgag acccgttatc atgagcgcgc cgagcgccgc aacgagcgcc
       61 gcgggcgtgg tgagcgcgcc gagcccgatc aggcgctccc gccaatcgga gaccatcacc
      121 tcgcgcggct cgcccgccag cgccgccaga cgatcgagca ccgggcgtgt cggttcggcg
      181 tagagatcgg ggagccggtc ggtgccgccg gcgcgttcga ggacatagcg gaacagtgac
      241 gtgcggacca cctcgctcac gccgccggac acgctgaggg cacgctcgca ggcggccacg
      301 tcgccgtgtc ggcatcccgt gaccacggat ccggtgtcgc gggccagccg gatccgcgcc
      361 gccggtgcga tcgtggcata cggggactcg agcgcctggc caccggccca tgcgcgcagc
      421 gtagcgtcgg cctcggccca gacggccttg cgggccgcgg agcggacgag ctccccgatg
      481 gcgccgccca ccaggcgatc cgacccagca aatgcgccgg gccctgcgcc ggtgaacgac
      541 acctcgagca ccggccgcgc gagcgagtcg aacaccaccc ggatcggccc ggccgagggc
      601 gacagcgcgc cgccgaactc cctcccggcc tcggccagcg cgaccggcac ctcacgttcg
      661 gcgaatggcc gggcagcccc gctcacaagg agcgtccatc cgccggccag ccgcacggtg
      721 tccaccgacg gaccggcgcg gcgcacggcc tgcagcgaat ccgcctcgcg ctgcagccgg
      781 atgcggatcg cctgcagctg ggctacgctc tgggcccgtg cggccgcagg ggcgccgagc
      841 gccagcacgc cggcacagag gagcgcgcgg ctagacatcg atcagcatcc agcggccgcc
      901 gaggacgtgc agggggctcc accgcccggt gagcgcatcc aggatccatc ctgtggcgtg
      961 gacgccgacc agttcaagcc cgatcgccac caccaccatc ccgaccaccg cccaggcgac
     1021 cggccgaccc acgagcgcga acatcgtcca cagggcgtgg ctcacgagga gcgccagcgc
     1081 gaaccgcagg atcaggccgc cggcgtaaag gtggagcccg tcaggcacta ccgcccgccc
     1141 cgcgaaggcc gccacggcga ttgcgaaggc cgcggcgagc cccgcgagca ccgtgccgcc
     1201 cgcgcccgca cgcatcagtg cataccgctc gcgacgcacc ggcagcgtaa gggcgtagac
     1261 ccaccacccg cgacggtcgg gccaccaggt gatgatcgac aggagcgtcg ccgctgtgaa
     1321 cgccagcacc ggcatcatca gcccccagaa ctgggcctcg accaggatct gctccggctc
     1381 ctgcaccggc ggcggcgcca ccgcgaggtg gagcgccccc cgcacgatca cgaacccgca
     1441 cgccgccagg aagagcagca tccaccgcga gtcgcgccag acctgcttga ggatcacccg
     1501 gaacatcagg cggccctccc cgcggcgcgg cggaacgagc gcagcagctc cacgaacgcc
     1561 tcctcgaggt cgaggtcgat cacctcgcgc accgtgacgc cggcacgccc gagcccggcc
     1621 gcagcgtcgg gggtccaatc gcgcaccacc cattcatcgg ccccggcgat ggtctcgctc
     1681 ccgatgacgg ccaccaggcc gatggcgggc accttcgaac ccgccgccac ccggatccgc
     1741 ttgatcccgt gcttgaacgc ctccatccgg acctgggcca cgagccgccc ctggtccatc
     1801 acccccaccc agtcacagat gcgttcgagc tcgtgtacca ggtggctcga gatgaacacg
     1861 gtggcgtgcg tccgctccac atactcgagg agcgcggcca gcacgtcgcg ccggaccaca
     1921 gggtcgagcc cgtcggtcgg ttcatcgagc accaggagct cgggcttctg ccccagcgcc
     1981 tggaggagca tcagcttggc ggtctctccc ttggacaggc ggccgatctc ggcgtcggac
     2041 ttgagcccga agtgtgccgc cagttccgcc gcccatgccg gatcccactt ccggtggaac
     2101 gccgagtgga attcgagcga ctgcgccacg gtgagggtcc ggtagaggtg cggccgttcg
     2161 ggcacatatc ccgtgcgccc cagcacctcg gggaggcgtg ccggaacggg gagcccgagc
     2221 agggacgccg cgccgctgaa tccggggtag agcccgagca gggtgcggat ggtggtggtc
     2281 ttgccggagc cgttggggcc gaggaagccg tagatggcgc cgaccgggac ggccagggag
     2341 acgtcggcca gggcgaagtt ccgccccagc cggaggccca ggccgcgggt ctcgatcgcg
     2401 agatcggtca tcgggtctcc ttgagttcgt cgtggagcgc ggcgcggatc tccgccggct
     2461 ggacgccgag tcgggccgcc tcggccagca tctgacggat gagctggcgg gcctgggcct
     2521 cgcgctggcg gccgcgccgc tcggtgctga ccgccttgac gtaggtgccg ctcccctgcc
     2581 gcatttccac gaatccctcc gcttcgaggt cgcgataggc ctgcaccacc gtggccggat
     2641 tgacccggat gcgggccgca agggcgcgga ccgactcgag cgccgcgccg ggggcgaggt
     2701 cgccggaggc gatggccacc ttgagccgct ccgcgatctg ggcgtagagc ggggtcggac
     2761 ttctgggatc gagccgttcg aacaccgggt caccgccgat tgtattggtg ttgccataca
     2821 gtacgacacg gcaggcgcgg cgtcaagagg ccgccccgac gcccggccgg tgccgcccgc
     2881 ccagtgtgac ctgcgcccta gccgcccctc ccccgcgccg tatatttgcc cttccgtagc
     2941 ttcccagcgg cgttccggcc cttccgggcc tgcacccttg caccaccccg aataccaacc
     3001 gaccccctga tcggccactc cggccagggg cgatcctgtt cagggacctg atgcctccga
     3061 cgactccact cggccgccgc ccgtacatct ccgcccggtt ccttcgcctg ctcgccaccg
     3121 gcctcctggg gctcgcggtg gtcacctgtt ctgacgacca cctcaacggt ccgtcg
//
LOCUS       DASUAP010000260         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_252975, whole genome shotgun sequence.
ACCESSION   DASUAP010000260 DASUAP010000000
VERSION     DASUAP010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252975"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VFU45_06840"
     CDS             complement(<1..1182)
                     /locus_tag="VFU45_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5358815.1"
                     /translation="MKLTPIPPAHSPSRPDDRRAAIAAGFLGWTLDAFDFFLVVLTLT
                     AIGREFHRPDADIVFSLFLTLAFRPVGAFIFGLLADRYGRRLPLMIDLVFYAVVEVLT
                     GLAPSYASFLVLRALFGIGMGGEWGVGASLVMEKVEPEKRGAVSGLLQQGYAAGYLLA
                     AVAYFAIFPRWGWRPLFFLGGLPALVALFIRVRVTESDAWRRTRHPDWRSLGRAVLAN
                     WRVFVVITLLMAMMNMTSHGTQDLFPTFLERSWGLDARGRALVTAVSMVGALIGGVMV
                     GRWSDRIGRRRAIGLALAGAVLMVPLWAFAPSLGLLVVGAFAIQFCVQGAWGVIPAHI
                     TELAPDGVRGFLPGFAYQCGALLASAFTYVQALFAARVSFAWTMALSAGVVFVIAGAV
                     AL"
     gene            1264..>2575
                     /locus_tag="VFU45_06845"
     CDS             1264..>2575
                     /locus_tag="VFU45_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEU5358816.1"
                     /translation="MTQHAAKPTFLDPSGRRKARLKAGIIAMGIITTLIVLVVFTGML
                     ISPVLPDLPLTQTAADSLGHVPRTARRPVRRTISVRAERERLASRQRLFQQLMKERRP
                     LRRSLRYSEIPLGRRLRADSALAAHRGAYDPTVVGFYVNWDDNSLASLRKNIDRLDWV
                     VAEWGLVRARGDSTPLAFQVDRRVLALAALAKQPPIILLMLTNANATGFDAGAVRRLV
                     GTERARRTTIREIVDTVTHYRLGGVTVDFEDLPPSMHPLLLRFLRELKPALEKEKLLL
                     TQAVPGDDPTWPVEDYARVNDRVFLMLYDEHDPSDDPGAVASQAWFDHHLARLLARVP
                     AGKALVGIGEYGYLWADTSESATELTFQDVMQLARDHAIHPTMDRDALNPTFHWDDPD
                     STSNIVWYLDGATAWNQIRAAERAGVAGIGIWRLGSEDPSLWSVL"
BASE COUNT          438 a          965 c          863 g          309 t
ORIGIN      
        1 gagcgctacc gcccccgcga tgacgaacac cacgcccgcc gagagcgcca tcgtccacgc
       61 gaacgacacc cgcgccgcga agagcgcctg cacgtaagtg aacgcactcg ccaggagcgc
      121 cccgcactgg taggcaaagc cggggaggaa tccgcgcacc ccatcggggg cgagctcggt
      181 gatgtgcgcc gggatgaccc cccacgcccc ctgcacgcag aactggatcg caaaggcacc
      241 caccaccagg agcccgagcg acggagcgaa cgcccagagg ggcaccatca gcaccgcgcc
      301 cgcgagcgcg aggcctatcg cccgccgccg cccgatccgg tccgaccacc gccccaccat
      361 cacgccgccg atgagcgccc cgaccatcga caccgccgtg acgagcgcgc gcccgcgggc
      421 atcgagtccc cagctccgct cgaggaaggt cgggaagagg tcctgggtgc cgtgggaggt
      481 catgttcatc atcgccatca gcagggtgat gacgacgaag acccgccagt tggccagcac
      541 ggcccgcccg agcgaacgcc agtcggggtg gcgggtgcgg cgccaggcat ccgactcggt
      601 cacccgcacc cggatgaaga gcgccaccag cgccgggagc ccgcccagga agaagagcgg
      661 ccgccacccc cagcgcggga agatcgcgaa gtaggccacc gccgccagca ggtatccggc
      721 cgcatatccc tgctgcagga gcccggagac ggcgccccgc ttctccggtt cgaccttttc
      781 catcaccaat gacgcgccga ccccccactc accgcccatc ccgatcccga agagcgcccg
      841 gaggacgagg aagctcgcgt agctcggcgc aagaccggtg agcacctcga ccacggcata
      901 gaagacgagg tcgatcatca gcgggaggcg ccggccgtag cggtcggcca ggagcccgaa
      961 gatgaaagca cccaccgggc ggaacgcgag cgtcaggaag agcgagaaga cgatgtcggc
     1021 gtcggggcgg tggaactccc ggccgatggc ggtgagggta aggacgacga ggaagaaatc
     1081 gaaggcgtcg agggtccagc cgaggaatcc ggcggcgatc gcggcgcggc ggtcgtccgg
     1141 gcgggaaggc gaatgggccg gcgggatcgg ggtgagcttc acgggtcact aagatagggc
     1201 gaaaggcccg ggaggccagc gcgtcccgat tggcgcggac ctctgggccg cctacctttc
     1261 ggcgtgaccc agcacgccgc caagccgacc ttcctcgacc cgtccgggcg ccgcaaggcc
     1321 cggctcaagg ccgggatcat cgcgatgggg atcatcacca cgctcatcgt gctggtggtg
     1381 ttcaccggga tgctcatctc cccggtcctc cccgacctcc ccctcaccca gacggcggcg
     1441 gactccctcg gccacgtccc ccgcaccgcc cggcgcccgg tccggcggac gatctcggtc
     1501 cgggccgagc gggagcggct cgccagccgc cagcggctct tccagcagct gatgaaggaa
     1561 cgccggccgc tccgccgctc cctccgctat tccgagatcc cactcggccg gcggctccgg
     1621 gccgacagcg cgctggcagc gcaccgcggg gcctatgacc cgacggtggt cggcttctac
     1681 gtcaactggg acgacaattc cctcgcctcg ctccggaaga acatcgaccg gctcgactgg
     1741 gtcgtggccg agtgggggct ggtgcgtgcg cggggggatt cgaccccgct cgccttccag
     1801 gtggaccgcc gggtgctggc gctcgcggcg ctcgccaagc agccgccgat catcctcctg
     1861 atgctcacca acgccaacgc caccggcttc gatgcgggcg cggtgcggcg cctggtcggc
     1921 accgagcggg cacggcgcac caccatccgg gagatcgtcg acaccgtcac ccactaccgc
     1981 ctgggcggcg tgacggtcga cttcgaggac ctcccgccgt cgatgcaccc gctgctcctc
     2041 cgcttcctcc gggagctcaa gcccgcgctc gagaaggaga agctgctcct gacccaggcc
     2101 gtcccgggcg acgacccgac gtggccggtc gaggactacg cgcgcgtcaa cgaccgggtc
     2161 ttcctgatgc tctacgacga gcacgacccg tccgacgacc ccggcgccgt ggcgagccag
     2221 gcgtggttcg accatcacct ggcccggctc ctcgcgcggg tgcccgccgg caaggcgctg
     2281 gtcggtatcg gcgagtacgg ctacctctgg gccgatacgt ccgagagcgc caccgagctc
     2341 acctttcagg acgtcatgca gctcgcgcgc gaccatgcca tccacccgac gatggaccgc
     2401 gacgcgctca acccgacctt ccactgggac gatcccgact ccaccagcaa catcgtctgg
     2461 tacctggacg gggccaccgc ctggaaccag atccgcgccg ccgaacgcgc cggcgtggcc
     2521 gggatcggca tctggcggct cggatcggag gacccgtcgc tctggtcggt gctgg
//
LOCUS       DASUAP010000261         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1111058, whole genome shotgun sequence.
ACCESSION   DASUAP010000261 DASUAP010000000
VERSION     DASUAP010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111058"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1024
                     /locus_tag="VFU45_06850"
     CDS             74..1024
                     /locus_tag="VFU45_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5358817.1"
                     /translation="MTDRVLIAGGAGFIGSHVVEAYAGAGWSVEVLDDLSSGKAANVP
                     SGIPLRRLDVRSPEARAAVAGGRYRLLVVLAAQVDVRVSVRDPLRDTDINVMGLINLL
                     EGAAEGGVRRVVFSSSGGVLYGEAEQVPTPETAEKRPISPYGVSKLAGEHALRVLGEL
                     RGFEVVALRYANVFGPRQDPNGEAGVMAIFTGRALRGEPLTIFGDGRQTRDYVFVRDV
                     ARANLLAGTAALPPVRDHDAIAFNIGTGIERSVLDVVTAVGRATGTTPAVVHAAARAG
                     ELQRSALDAGRAGAVLGWKPEVRFEEGLSALVQWMRTEAR"
     gene            1021..1734
                     /locus_tag="VFU45_06855"
     CDS             1021..1734
                     /locus_tag="VFU45_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU5358818.1"
                     /translation="MILQLGGAVPRSSLDLVLHSGTETIVVLLVTGVFSLVSWFIIVQ
                     KWWQFRRLRAQGDRFFAEIERTTRLRDAYHAVMKLPPSPYHRIFREAINFFSELRPGA
                     LRDAGDVPKGSLSVTQLEALKMMLGKDIAAERDVASQYIPWLATIGSVSPLMGLLGTV
                     LGVMSAFIGISAGGSGNIAAVAPGVAEALVTTVAGLATAIPAVIGYNLYASRVGLFSA
                     EMEGFADELIGTMAREGLI"
     gene            1736..2179
                     /locus_tag="VFU45_06860"
     CDS             1736..2179
                     /locus_tag="VFU45_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HEU5358819.1"
                     /translation="MAGGLGSSGFRGRHGPPPLNADINVTSLVDVAFVLLIIFMIAAP
                     MMQGGVDVQLPRAQARPLTPKEQGLVVSVDREGRIFVDETQVSYNDFKVTFRGLVATR
                     HPSGVFLRADKRVAYGDVVRVLAIIRGSGVTDVGLIAEDEETPTQ"
     gene            2185..2565
                     /locus_tag="VFU45_06865"
     CDS             2185..2565
                     /locus_tag="VFU45_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358820.1"
                     /translation="MRLRPPRGNLALGVSGTVVVHATVVVLLVALAPRPRIEPPVYAV
                     NLVAAPAPAPETPKPTVNQPAPPPQPVAPTKPVKSSRPAPPPPKPAPKPAPSKSEARS
                     GRRHRSRSPRPPARRRAPGPTRSP"
BASE COUNT          342 a          944 c          947 g          386 t
ORIGIN      
        1 ccttcggggc cgccgcgccg atggcgtttc cggcggggta cggctgcggg ccggccccgg
       61 ctagattgac aggatgacag atcgcgtgct catcgccggc ggcgccggct tcatcggttc
      121 gcacgtagtg gaggcctacg ccggcgcggg gtggtcggtc gaggtgctcg acgacctctc
      181 gtcgggaaag gccgccaacg tcccctcggg catcccgctc cggcgcctcg acgtgcgctc
      241 gcccgaggcc cgcgctgcgg tcgccggcgg ccggtatcgc ctgctggtcg tcctggcggc
      301 gcaggtggac gtgcgcgtgt cggtccgcga cccgctccgc gacaccgaca tcaatgtgat
      361 ggggctcatc aacctgctcg agggcgcggc ggagggcggg gtccggcggg tggtgttctc
      421 ctcgagcggc ggggtgctct acggcgaggc ggagcaggtg ccgacgccgg agaccgccga
      481 gaagcggccg atctcgccgt acggggtgag caagctcgcc ggggagcacg ccctgcgggt
      541 gctgggcgag ctccggggct tcgaggtggt ggcgttgcgg tacgccaacg tcttcggccc
      601 gcggcaggac cccaatggcg aggcgggcgt gatggcgatc ttcaccgggc gcgcgctccg
      661 gggggagccg ctcaccatct tcggcgacgg gcgccagacc cgcgactacg tgttcgtgcg
      721 ggatgtggcg cgggccaacc tgctcgccgg cacggccgcg ctgcccccgg tccgcgacca
      781 cgacgcgatc gcgttcaaca tcggcaccgg catcgagcgg tcggtcctcg acgtcgtcac
      841 ggccgtggga cgcgccaccg gcaccacgcc cgccgtggtc cacgctgcgg cgcgggccgg
      901 tgagctccag cggagcgcac tcgacgccgg gcgcgccgga gcggtgctcg gctggaagcc
      961 ggaagtacgg ttcgaagagg ggctctcggc cctcgtccaa tggatgcgaa cggaggcacg
     1021 gtgatcctgc agctcggcgg tgcagtcccg cggtcgtcgt tggacctggt gctccactcg
     1081 ggcaccgaga ccatcgtggt gctcctggtg acgggcgtgt tctcgctcgt atcctggttc
     1141 atcatcgtcc agaagtggtg gcagttccgg cggctccggg cgcaggggga tcggttcttc
     1201 gccgagatcg agcgcaccac ccgccttcgt gacgcctacc atgcagtgat gaagctcccg
     1261 ccttcgccgt accatcggat cttccgcgag gccatcaact tcttctccga gctccgtccc
     1321 ggcgccctgc gcgacgccgg cgacgtaccg aaggggtccc tcagcgtcac ccagctcgag
     1381 gcgctcaaga tgatgctcgg caaggacatc gccgccgagc gcgacgtggc gagccagtac
     1441 atcccgtggc tcgccaccat cgggtcggtg agcccgctga tggggctcct cggcaccgtt
     1501 ctcggcgtga tgagcgcgtt catcgggatc tcggccggcg ggtcggggaa tatcgcggcg
     1561 gtggcgccgg gcgtcgccga ggcgctggtg accaccgtgg cggggctggc gacggcgatc
     1621 ccggcggtga tcgggtacaa cctctacgcc agccgggtgg ggctcttcag cgccgagatg
     1681 gaagggttcg ccgacgagct gatcggcacc atggcccggg aagggctgat ctaggatggc
     1741 cggcggcctc gggtcctccg ggttccgcgg gcggcacggc ccgccgcccc tcaacgccga
     1801 catcaacgtc acctcgctgg tggacgtggc gttcgtgctg ctcatcatct tcatgatcgc
     1861 ggcgccgatg atgcaaggcg gcgtggacgt ccagctcccg cgcgcgcagg cccggccgct
     1921 caccccgaag gagcagggcc tggtggtgag cgtcgaccgc gaggggcgca tcttcgtcga
     1981 cgagacccag gtgtcgtaca acgacttcaa ggtcaccttc cgcggcctgg tggccacccg
     2041 ccacccctcc ggggtcttcc tgcgggccga caagcgcgtc gcctacggcg acgtcgtgcg
     2101 ggtgctcgcc atcatccgcg ggtcgggcgt caccgacgtc ggcctcatcg cggaggacga
     2161 ggagaccccc acgcagtgag ggccgtgcgc ctccgcccgc cgcgcgggaa cctcgccctc
     2221 ggcgtctccg ggacggtcgt cgtccacgcc acggtggtgg tactgcttgt ggcgctggcg
     2281 ccacgtcccc gcatcgagcc cccggtgtat gccgtcaacc tggtggcggc gccggccccc
     2341 gcgccggaaa cgcccaagcc gacggtcaac cagccagcgc cgccgcccca gccggtggca
     2401 ccgaccaagc cggtcaagtc gtcgcggccg gcgccgccgc cccccaagcc cgctccgaag
     2461 ccggcgccct cgaagagcga agcccggtcc ggcaggcgcc accgcagccg gtcaccgcgg
     2521 cccccggcga ggcgccgagc accgggaccg acgcgatcac cgtgaagacc cccgggctcg
     2581 atttcccgtt ccccgattac ctccgcaaca tcgtctcgc
//
LOCUS       DASUAP010000262         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_361938, whole genome shotgun sequence.
ACCESSION   DASUAP010000262 DASUAP010000000
VERSION     DASUAP010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361938"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..612)
                     /locus_tag="VFU45_06870"
     CDS             complement(16..612)
                     /locus_tag="VFU45_06870"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEU5358821.1"
                     /translation="MTDIAAQYGQLLVRLDRWSADAAARHPGVIPCRLGCTACCHGPF
                     DISAADAALLRSGLAALAPSVRAAVRDRARTLLRRMGQEAPGWGPPWHIEDIGEDRFD
                     DVVEALADEPCPLLGADGGCLTYAHRPAVCRMMGLGMHTPSGDIDNACPIQHQFPAYA
                     ALPPQPFDLEAFEQAEERLIARAGGAETTIAAVAGEEK"
     gene            complement(609..983)
                     /locus_tag="VFU45_06875"
     CDS             complement(609..983)
                     /locus_tag="VFU45_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEU5358822.1"
                     /translation="MDRTFAALGALSAGLAVAAGAFGAHALKGHLSPEDLAVFETGAR
                     YQMYHALALLAVAWAASRWPVPAVSVAGWLFVAGTILFSGSLYALALTGVRWLGAVTP
                     LGGVAFLAGWAALALGAWRGRA"
     gene            1320..>3498
                     /locus_tag="VFU45_06880"
     CDS             1320..>3498
                     /locus_tag="VFU45_06880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEU5358823.1"
                     /translation="MQLWLISTVCWWVIGIPFSTIWWLMNPEQLRWVLICYAWEVPIV
                     GWLGLVGGAWLLWRRIIRRHRAGHLDSSRDVAALPRNVGLLALAISSVGYFLGAVQIR
                     HFAALPYIEAAKIVTQGVVLGLVLGAAAYLTAEACVRRSALPAADLHGTGLYARVMAV
                     GAAVAIGLGMPVFLLGLSREQARLEAIRGGQLEDALAATAGDATALQQALSAVGGHTS
                     AFVVDRATGRIVAGAGAPALLEDDRIDDVGRILTGSDGWFVSRQWTDKVVATRELAGA
                     RPPGLVLVAVSPFQDYGRALARTALLALIVLLVAMAVGYLVLRAFALSLVRPLARIGA
                     AAADMATGRIDVAEVGYPGTDEIAGLASAFDRMAARVRTDEQELRSAYQRLISTQTQL
                     LQEAKLSSVGRLVSGVAHELNNPLTAILHLTDEVAGMPHLADEDRELLRVVHQQAQRA
                     RAIVRDLLAAVRGREVRRERFGVRSLIDGVLAGLRATTGAPMARVTVDVAADLPDLDA
                     DRAGVEQILVNLVQNASHAAGPEGVVTVRVTASDTGVTFTVEDTGPGMAPEVLPRIFE
                     PFFTTKREGEGTGLGLFVTLGIVEAHGGTIRAENLLPPGSGARVTVVLPPGGAADATG
                     AGQERTAAPMAEAGRVLLVDDESAIRLAVRRWFVRQGWEVVEATDGGDALVQALAAPH
                     GGFDLVLTDLKMPGLSGIELVDRLAVLRPDLHARIVIMTGDVAS"
BASE COUNT          446 a         1233 c         1279 g          540 t
ORIGIN      
        1 cttacttctt acttcttact tctcctcccc cgccaccgcc gcgatggtcg tctccgcgcc
       61 ccccgcgcgg gcgatcagcc gttcctccgc ctgctcgaac gcctccaggt cgaatggctg
      121 gggcgggagt gcggcgtagg cagggaattg gtgctggatg gggcaggcgt tgtcgatgtc
      181 accgctcggg gtatgcatac cgagccccat catccggcac accgcagggc ggtgcgcgta
      241 cgtgaggcag ccgccgtcgg cgccgaggag cgggcagggt tcgtccgcca gtgcctccac
      301 cacgtcgtcg aaccggtcct caccgatgtc ctcgatatgc cagggcgggc cccaccccgg
      361 tgcctcctgt cccatccgcc ggagcagtgt gcgggcacga tcccggacgg cggcccgcac
      421 cgatggcgcc agcgcggcga gcccgcttcg gaggagggcg gcatcggcgg cggagatatc
      481 gaatggccca tgacagcacg ccgtgcagcc gagacggcag gggatgacgc cggggtgcct
      541 ggccgcggcg tcggccgacc accggtcgag ccgcaccagg agctggccgt actgggccgc
      601 gatgtcggtc acgcccggcc ccgccaggcg ccgagggcca gggcggccca gcccgccagg
      661 aaggccacgc cgcccagcgg cgtgacggcc ccgagccagc ggacgccggt cagggcgagc
      721 gcgtagaggc tccccgaaaa gaggatggtg ccggccacga agagccatcc cgccacgctc
      781 accgcgggga caggccagcg cgacgcggcc cacgctacgg ccaggagcgc gagtgcgtgg
      841 tacatctggt agcgggcgcc ggtctcgaag accgcgaggt cctcgggcga gaggtggccc
      901 ttgagcgcgt gggcgccgaa cgcccccgcg gccacggcga gcccggccga gagtgcgccg
      961 agtgctgcga atgtgcggtc catcggggta agatataccg ctggccccgc atgcaggcgt
     1021 ggtgcatttt ggggagggct tagggcttag ggcttagggc ttagggctta gggcttgggg
     1081 cttggggctt agggcttaga gcgttgggcg ttgggcgtag ggctcagagc ttgcgggcga
     1141 gcgacggccc cgctccccgc tcacatccca ctcttgacgt ctcctgtacc ctgtcccctt
     1201 ttcccttctc acttcttact tctcacttct tacttctcac ttcttacttc tcacttctta
     1261 cttctcactc cgacccgccc ctgcctcctt cccttgacgc gctcggtcgc cgactggcgg
     1321 tgcagctctg gctcatcagc acggtctgct ggtgggtcat tggcatcccg ttctcgacga
     1381 tctggtggtt gatgaacccg gagcagctcc gctgggtgct catctgctac gcctgggagg
     1441 tgccgatcgt cggatggttg ggcctggtgg gcggggcatg gctcctctgg cggcggatca
     1501 tccggcgcca ccgcgccggg cacctcgatt cctcccgcga cgtggcagcg cttccccgga
     1561 acgtcggcct cctggccctc gcgatcagct ccgtcggcta cttcctcggc gccgtccaga
     1621 tccggcactt cgccgccctc ccctacatcg aggcggcgaa gatcgtgaca cagggggtgg
     1681 tgctcgggct ggtgctgggt gcggcggcct acctgacggc ggaggcgtgc gtccgccggt
     1741 cggcgctgcc cgcggccgac ctccacggga ccggcctcta cgcccgcgtg atggccgtcg
     1801 gcgccgccgt ggcgatcgga ctggggatgc cggtgttcct gctgggactg agccgggagc
     1861 aggcgcgact cgaggccatc cgtggcggcc agctcgagga cgccctcgcg gccaccgccg
     1921 gcgacgccac agcgctccag caggcgctca gcgccgtcgg gggccacacg tcggcgttcg
     1981 tcgtcgaccg cgccaccggg aggatcgtcg ccggcgccgg ggccccggcc ctgctcgagg
     2041 acgaccggat cgatgatgta gggcggatcc tgaccggctc cgacggatgg ttcgtgagcc
     2101 gtcagtggac cgacaaagtg gtggcgaccc gcgaactggc cggcgcgcgc cccccggggc
     2161 tggtcctggt ggccgtctcg ccgttccagg actacggccg cgccctggcg cgcacggcgc
     2221 tcctggcgct catcgtgctc ctcgtggcga tggccgtggg ctatctcgtg ctccgggcgt
     2281 tcgccctgag cctggtgcgg ccgctcgccc ggatcggcgc ggcggcggcg gacatggcca
     2341 ccggccggat cgacgtggcc gaggtcgggt atcccggcac cgacgagatc gccggcctgg
     2401 cgtcggcgtt cgaccgcatg gccgcccggg tgcggaccga cgagcaggag ctccggtcgg
     2461 cctaccagcg gctcatctcc acccagacgc agctgctgca ggaggccaag ctctcctccg
     2521 tcgggcggct ggtgtcgggg gtggcacacg aactcaacaa tccgctcacc gcgatcctgc
     2581 acctgaccga cgaggtggcg gggatgccgc acctcgccga cgaggaccgg gagctgctgc
     2641 gcgtcgtcca ccagcaggcg caacgggccc gggccatcgt gcgcgacctg ctcgccgccg
     2701 tgcggggtcg ggaggtgcgc cgggagcggt tcggggtccg gtccctcatc gacggagtgc
     2761 tcgccggcct gcgggccacc accggcgcgc cgatggcccg ggtgacggtc gacgttgccg
     2821 ccgacctccc cgacctcgat gcggaccggg caggggtcga gcagatcctg gtcaacctgg
     2881 tccagaatgc gtcgcacgcc gctgggccgg agggtgtggt cacggtccgg gtcacggcct
     2941 ccgacaccgg cgtcaccttc accgtggagg acaccggccc ggggatggcg cccgaagtgc
     3001 tgccccggat cttcgagcct ttcttcacca ccaagcgcga aggagagggg accgggctcg
     3061 gcctcttcgt gaccctgggc atcgtcgagg cccacggcgg gaccatccgg gcagagaacc
     3121 tcctgccgcc cggttcgggg gcacgggtga ccgtcgtgct gccgccgggc ggggccgccg
     3181 acgccacggg cgcggggcag gaacggaccg cggctccgat ggctgaggcc gggagggtgc
     3241 tcctggtcga tgacgagtcc gccatccggc tggcggtccg gcgctggttc gtgcgccagg
     3301 gatgggaggt ggtggaggcg acggacggcg gggacgccct ggtgcaggcg ctcgccgcgc
     3361 cccacggggg attcgacctg gtgctgaccg acctgaagat gccggggctt tcagggatcg
     3421 agctggtcga ccggctggcg gtgctgcgcc ccgaccttca cgccaggatc gtcatcatga
     3481 ccggcgacgt ggcctcgc
//
LOCUS       DASUAP010000263         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_648490, whole genome shotgun sequence.
ACCESSION   DASUAP010000263 DASUAP010000000
VERSION     DASUAP010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648490"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2382
                     /locus_tag="VFU45_06885"
     CDS             <1..2382
                     /locus_tag="VFU45_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5358824.1"
                     /translation="AGPWYAGTLREAALGGGPEADVNWNNVVGDTLPVIVTRASDGWV
                     LFADTDGDGSLRNERPVHDYLVARETFGWAPRGHAPALTMAANISTQGGAPALDLFFD
                     TSGHGTHVAGIAAGHDLYGVPTFDGVAPGAQVLGLKIADDAHGGISVTGAMLDALAYA
                     IRFAADHRLPLVVNMSFGVGNEPGVVPRIDRLIDSVLARHPDVTFAVSAGNDGPGVSS
                     LGFPGSARRVLSVGATLPAVFFTRDRGEPDVLAYFSSRGGESARPDIVTPGMAYSLVP
                     RWHRGQEVLQGTSMASPYAAGLAAVLASALAERRQPIEARRIRQALMATAQPIAGHSA
                     IGQGDGLPNLGRAWAWLAGRFRYPEVDVAVGAPQAGRTNAAYRPAGLLGPGDTLQTFV
                     LFRPPGDSAPLSLTFRTDTTWLVPPDAVTLRGTPVPVTLRYRPGALRAPGLHVGVVTG
                     WGDDTLAGALVRLENTIVTPIAIGSTFEQRTAQLAGGFERRWFFSADTGRPFQVDIAA
                     AGPDEVVGAFLHEPGGMPFRDGHEREASVAGGVASFNVDAADVVGGVYEVDAATPPGG
                     AASPRVKVTVAPLVLSGTRDAQGIRADVTNPTALPVDVALGGRIVGAVRSVPLVERGS
                     RRVTIPFVAPEWARAVRIDIQMDPAEWGRFTDFGLDILDPAGRELGELALNYAVGRFA
                     AKLPPHDGPTPITAALSPGLATEGDTAAWNATLSVRLYADPDSAVPFRAGTAAPVTVA
                     PGASATVRFPMPVLGWTLDPGFSPLGVLTAQVAGGPVWTRELQLGAPMGLMMR"
     gene            2382..3755
                     /locus_tag="VFU45_06890"
     CDS             2382..3755
                     /locus_tag="VFU45_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEU5358825.1"
                     /translation="MDRTVRVAAGQGFWGDWLEAPVRQVLGGPIDYLMMDYLAEVTMS
                     IMQKQKSRDPKLGYAKDFPPLMERLLPELVARGVRVTSNAGGVNPRACAEAVVASARK
                     LGLGGKVKVGLVTGDDILPRLDELIAKGHALNDMDTGRPLSDIRDKVVSANAYLGMAP
                     VVTALDRGAGIVITGRVTDTGLTLGPLVHEFGWAADDWDRIAAGTIAGHIIECGAQAS
                     GGNLLKDWRRVPGMADPGFPIVEAAPDGTFVVTKHPRTGGVVSVASVSEQLVYEMGDP
                     HNYITPDGVADFTTIHLAPAGKDRVAVRGIRGRPRTGLLKVSVAYFYGYKAVGTLVYS
                     WPDAYDKARAADKILRQRLQDLGLEFEQVLTEFVGVDATHGRLSGPPSAGLAEVQLRV
                     GVRARERAPVERFTREIAPLVLTGPPSVTGFAGGRPAVEEIVAYWPALVDRREIEPFV
                     RVEILEA"
     gene            3795..4235
                     /locus_tag="VFU45_06895"
     CDS             3795..4235
                     /locus_tag="VFU45_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358826.1"
                     /translation="MAKVKRARAASKGRARSAHTTRRRTAEASVQLRWLAHARSGDKG
                     DTANVGLIALRPGWYELLRREVTVARVARHFKGMVSGVERFELPNLHALNFLLHGALD
                     GGGTLSLKTDAQGKVYSTALLRMRIPVPADVRKGLPRAALRAEG"
BASE COUNT          561 a         1601 c         1545 g          605 t
ORIGIN      
        1 gcgggcccct ggtacgccgg cacgctccgc gaagcggcgc tcggcggcgg gcccgaggcc
       61 gacgtcaact ggaacaacgt cgtcggcgac acgctcccgg tcatcgtcac gcgcgccagc
      121 gacggctggg tgctctttgc cgacaccgac ggcgacggga gcctccgcaa cgaacggccg
      181 gtccacgact atctggtggc ccgcgagacc ttcggctggg cgccacgggg ccacgcgccg
      241 gccctgacga tggcggccaa catctccacg cagggcggcg cgcccgccct cgacctcttc
      301 ttcgacacca gcggccacgg cacccacgtc gccggcatcg ccgcgggaca cgacctctac
      361 ggcgtgccga cgttcgacgg cgtggcgccg ggcgcccagg tgctgggcct caagatcgcc
      421 gacgacgcgc acggcggcat ctcggtcacc ggggcgatgc tcgacgcgct ggcgtacgcc
      481 atccgcttcg cggccgacca ccgcctgccc ctcgtcgtca acatgagctt cggcgtcggc
      541 aacgagccgg gcgtcgtgcc acgcatcgac cgcctcatcg actcggtcct ggcccggcac
      601 cccgacgtga cattcgccgt gagcgccggc aacgatggcc cgggcgtctc gtcgctcggc
      661 ttccccggct cggcccgccg ggtgctctcg gtcggcgcga cgctgcccgc cgtcttcttc
      721 acccgcgatc gcggcgaacc tgacgtcctg gcgtacttca gttcgcgcgg cggcgagagc
      781 gccaggcccg acatcgtgac gccggggatg gcctattcgc tcgtgccgcg ctggcaccgg
      841 ggtcaggagg tgctccaggg caccagcatg gcgtcgccgt acgcggcggg gctcgcggcg
      901 gtcctcgcct ccgcgctcgc ggaacgccgc cagccgatcg aggcccggcg gatccgccag
      961 gcgctcatgg ccaccgcgca gccgattgcc gggcacagcg cgatcggcca gggcgacggg
     1021 ctccccaacc tgggccgcgc gtgggcatgg ctcgcggggc ggttccgcta ccccgaggtc
     1081 gatgtcgcgg tgggtgcgcc gcaggcgggg cggaccaacg ccgcctaccg gcccgccggg
     1141 ctcctcggcc ccggcgacac gctccagacg ttcgtcctct tccgcccacc cggcgattcc
     1201 gccccgctct ccctgacgtt ccgcaccgat accacgtggc tcgtgccacc ggatgccgtc
     1261 acgctgcgcg ggacgcccgt cccggtcacc ctgcgttacc gcccgggcgc gctccgcgcc
     1321 cccgggctcc acgtcggcgt cgtcaccggc tggggcgatg acacgctggc cggcgccctg
     1381 gtgcggctcg agaacacgat cgtgacaccg atcgcgatcg ggtcgacgtt cgagcagcgg
     1441 acggcgcagc ttgccggcgg ctttgagcgc cgctggttct tttccgccga taccggccgg
     1501 ccgttccagg tcgatatcgc cgccgccggc ccggatgaag tggtcggcgc cttcctccat
     1561 gagccgggcg gaatgccgtt ccgcgatggc cacgagcgcg aagcgtcggt cgccggcggc
     1621 gtcgcgtcgt tcaacgtcga cgccgcggac gtcgtcggtg gcgtctacga agtcgatgcg
     1681 gcgacgccgc cgggcggggc cgcgtccccc cgggtcaagg tcacggtggc gccgctcgtg
     1741 ctctctggca cccgcgacgc ccagggcatc cgggccgacg tgacgaaccc gaccgccctg
     1801 ccggtcgacg tggccctcgg cggacggatc gtgggcgccg tccggtcggt gccgctcgtc
     1861 gagcgcgggt cccggcgggt gacgatcccg ttcgtcgcgc cggagtgggc ccgcgccgtc
     1921 cggatcgaca tccagatgga tcccgcggag tggggccgat tcaccgactt cggcctcgat
     1981 atcctggacc cggcggggcg ggagctcggt gagctggcgc tcaactatgc ggtggggcgc
     2041 ttcgccgcaa agttgccgcc ccacgacggg cccacgccga tcacggcggc gctttcaccg
     2101 ggccttgcca ccgagggcga caccgccgcg tggaacgcca ccctctcggt gcggctgtac
     2161 gccgatcccg attccgccgt cccgttccgt gcggggaccg cggcgccggt gacggtggcc
     2221 ccgggtgcga gcgccacggt gcggtttccg atgcccgtgc tcgggtggac cctggatccc
     2281 ggcttctcgc cgctcggcgt actcaccgcg caggtcgccg gaggtccggt gtggacccgc
     2341 gaacttcaac tgggggcccc gatggggctg atgatgcgct gatggatcga accgtacgcg
     2401 ttgccgccgg ccagggcttc tggggcgact ggctcgaggc tcccgtccgc caggtgctcg
     2461 gcggcccgat cgactatctg atgatggact acctcgccga ggtgacgatg tccatcatgc
     2521 agaagcagaa gtcccgcgac ccgaagctcg gctacgccaa ggattttccg ccgctgatgg
     2581 agcggctcct gccggagctc gtggcccggg gcgtgcgcgt cacctccaac gcggggggcg
     2641 tcaacccgcg ggcctgcgcc gaggcggtgg tggcctcggc gcggaagctg ggcctcggcg
     2701 ggaaggtcaa ggtcggcctc gtcacgggcg acgacatcct gccgcgcctc gacgagctga
     2761 tcgcgaaggg acacgcgctc aacgacatgg acaccggacg ccccctttcg gacatccggg
     2821 acaaggtcgt ctcggccaac gcctacctcg gcatggcccc ggtcgtgacg gcgctcgacc
     2881 ggggggccgg catcgtgatc accgggcgcg tcaccgacac cgggctcacc ctcgggccgc
     2941 tggtccacga attcggctgg gcggcggacg actgggaccg catcgccgcc ggcaccatcg
     3001 ccggtcacat catcgagtgc ggcgcccagg cgtcgggcgg caacctcctc aaggactggc
     3061 ggcgcgtccc cgggatggcc gaccccggct tcccgatcgt ggaggcggcg cccgacggca
     3121 cgttcgtggt gaccaagcac ccgcgcaccg gcggggtcgt gtcggtggcg agcgtgtccg
     3181 agcaactcgt gtatgagatg ggcgatccgc acaactacat cacccccgac ggcgtcgccg
     3241 atttcaccac catccacctc gcgccggccg ggaaggatcg cgtggcggtg cggggcatcc
     3301 gaggccggcc gcggacgggc ctgctcaagg tgtcggtggc gtacttctac ggctacaagg
     3361 ccgtcggcac gctggtgtac tcgtggcccg atgcctacga caaggcgcgc gcggccgaca
     3421 agatcctgcg ccaacggctc caggacctcg ggctcgagtt cgagcaggtg ctcaccgagt
     3481 tcgtcggcgt cgacgccacc cacggtcggc tgtcagggcc gccgagcgcc gggctggcgg
     3541 aagtccagct ccgcgtcggc gtgcgggccc gggagcgcgc gccggtcgaa cgcttcaccc
     3601 gggagatcgc gccgctggtc ctcaccgggc cgcccagcgt caccggcttc gccggcggcc
     3661 ggccggcggt cgaggagatc gtcgcgtact ggcctgcgct cgtcgaccga cgcgagatcg
     3721 aaccgttcgt gcgggtggag atcctcgagg cgtgaccggg tgggccgaac ggacgtgacc
     3781 gaaaaggggg gaccgtggca aaggtgaagc gggcacgcgc ggcatcgaaa ggcagggcgc
     3841 gatcggcgca cacgaccagg cgtcggaccg ccgaggcgtc ggtccagctc cgctggctcg
     3901 cgcacgcccg ctccggcgac aagggcgaca ccgccaacgt gggcctcatc gcgctccgcc
     3961 ccggctggta cgagctcctc cgccgcgagg tcacggtcgc gcgggtggca cggcacttca
     4021 aggggatggt gtcgggggtg gagcgcttcg agctgcccaa cctccacgcg ctcaatttcc
     4081 tgctccacgg cgcgctggac gggggcggca cgctgtcgct caagaccgat gcccagggca
     4141 aggtctattc gacggccctg ctccgcatgc ggatcccggt ccccgcggat gtgcggaagg
     4201 gtttgccgcg cgcggcgctc agggccgaag ggtgagccgc gtgccgacgc tctccgcccg
     4261 gctcgccgcc ggcgtcctca ccctcaccct cgatcgcccg gacaagcgga ac
//
LOCUS       DASUAP010000264         3223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_458300, whole genome shotgun sequence.
ACCESSION   DASUAP010000264 DASUAP010000000
VERSION     DASUAP010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458300"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFU45_06900"
     CDS             complement(<1..731)
                     /locus_tag="VFU45_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEU5358827.1"
                     /translation="MRATGERSWPTAPTIVAEALVSVVDPLALWLRSGPVGTPRSVAD
                     TIALAAAAPPAADAPPSWLRPEQRTAFATLLTLIRRHRGALLADAPGTGKTFTALAVA
                     GAYPRGAVAALVPATLKPQWGARAKALGVALEVISHEEVSRGHCPAGSAQLVIVDESH
                     RFRNPATKRYRTIAPWMVGRDALFLTATPVVNDPVDLAHQLLLAVRHDALAPLGIPSL
                     AATLARGHGHPALGDLVVAREPPDGA"
     gene            complement(679..>3223)
                     /locus_tag="VFU45_06905"
     CDS             complement(679..>3223)
                     /locus_tag="VFU45_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358828.1"
                     /translation="FRLGRDRLAQALEGPGPADRESLALLQLTRILFLYFVQAKGWLD
                     GRSDFLARSLDDCLRRGRHPQHDLLHPLFFGTLNRPVARRSQSTLRFGRIPFLNGGLF
                     EPHPIERRVRAAAPTAVWRQVFDELFERFHFTVTEGDTIGIAPDMLGRVFEGLMSPDT
                     RRLTGTFYTPPTLVSRMVDAGLAALLQAQLAIPADRAFRLLADPDPAARRFLADITVL
                     DPAAGSGAFLLGALERLSGATLSDRRNADTARALTTARRAVLRRNLFGVDLSATAVRL
                     AELRLWLAVVRDDPAGPGEEVEPLPNLDCTIRQGDSLRDPLPHGADPAFDAEPLARAR
                     SLAMIATGPEKRRATDRLRTEERRAACATLTAMEREFDHGIAECLSDARAPTLFERRR
                     GLDRDLRARLAALRSDRATTRDAMRRLRRDGELPWFHYQAHFADVFARGGFDLIVGNP
                     PWVRSEAIPPPTRRELVRRYRWWKGAGGTGYSHQADLSVAFVERALELVAPGGAVTFL
                     VPAKLATAAYATPARAALCARHNVRVVSDLSTDPDASFGATVYPLALVVTKAKPAAED
                     RTRLSLAPDGATVSHASLGGGGPWVLSDPSAGRLRAALRERFPVLADTAPPQLGVKTG
                     ENRAFIDPPDAIDARFVRVALRGREIRPFAIRPTHRLLWTHGSDGRPLPTLPPDVLTY
                     LEPWRERLEQRADRRRGQWWQLFRVGPAFARHRVVWPDLARRLAACALTGRPDVIPLN
                     SCYVATMPSAPQAHALAAWLNAAPLRACARLGADPAMSGFARFNARSVGQLPWPEAAA
                     TDRALITFGERGSKGEDDTAALDDRAADLLQLDARDRRALLADRTHDRR"
BASE COUNT          463 a         1107 c         1232 g          421 t
ORIGIN      
        1 gctccgtccg gcggctcccg ggccacgacg agatcgccga gcgcggggtg tccgtgtccc
       61 cgggcgaggg tggcagcgag cgacgggatc cccagcggcg ccagcgcgtc gtggcgcacc
      121 gccagcagga gctgatgggc caggtcgacc ggatcgttca cgacaggtgt cgccgtgagg
      181 aagagcgcat cccgtcccac catccacggc gcaatggtcc ggtaccgctt ggtggccggg
      241 ttccggaacc ggtggctctc gtcgacgatg accaactgcg ccgagcctgc ggggcaatgc
      301 ccgcggctca cctcctcgtg gctgatgacc tcgagggcca ctcccagcgc cttcgcacgg
      361 gcgccccatt gcggcttgag cgtggcgggc accagcgcgg cgacggcgcc tcgggggtag
      421 gcgccggcga cggcgagggc cgtgaacgtc tttccggtgc cgggcgcatc ggcgagcagc
      481 gcgccgcggt gccggcggat gagcgtcagc agggtcgcga acgcggtccg ttgttcgggc
      541 cggagccagc tcggaggtgc gtccgcggcg ggcggggccg ccgcggcaag cgcgatcgtg
      601 tccgccaccg atcgaggcgt acccaccggc ccggaccgaa gccacagtgc cagcgggtcg
      661 acgaccgaga ccagcgcttc agcgacgatc gtgggtgcgg tcggccagga gcgctcgccg
      721 gtcgcgcgca tcgagctgaa ggaggtcggc cgcgcggtcg tcgagcgccg cagtgtcgtc
      781 ttcacccttt gatccccgtt ctccgaatgt gatcagtgcc cggtcggtcg ccgccgcctc
      841 gggccagggc aactggccca cgctgcgggc gttgaatcgc gcgaagccgc tcatcgcggg
      901 gtcggcgccg agccgggcgc aggcccggag cggggcggcg ttgagccagg cggccagtgc
      961 gtgcgcttgc ggcgccgacg gcatggtggc cacatagcag ctgttgaggg ggatgacgtc
     1021 cgggcgtccg gtcaacgcgc acgcggcgag ccggcgcgca aggtccggcc acaccacccg
     1081 gtgccgcgcg aaggccggcc ccacgcggaa gagctgccac cactggcctc tgcgacggtc
     1141 ggcacgctgc tccagccgtt ccctccacgg ttcgaggtac gtcagcacgt cgggcggcag
     1201 cgtcggcagc ggccggccgt cgctgccgtg ggtccagagc agccggtgcg tgggccgaat
     1261 ggcgaacggc ctgatctccc gtccgcggag ggccacgcgt acgaagcggg catcgatcgc
     1321 atccggcggg tcgatgaatg cgcgattttc gccggtcttg acgccgagct gtggcggcgc
     1381 cgtgtcggcg aggaccggga agcgctcgcg gagcgcggcg cggagcctcc ccgcgctcgg
     1441 gtcggacagc acccaggggc cgccgccccc gagcgacgca tgcgacacgg tggcgccgtc
     1501 cggcgcgagc gaaagccggg tccggtcctc ggccgccggc ttagccttgg tgaccaccaa
     1561 ggcgagcggg tacaccgtgg cgccgaagga cgcatccgga tcggtcgaga ggtcggacac
     1621 cacacgtacg ttgtggcgtg cgcagagcgc cgccctcgcc ggcgtggcgt aggcggcagt
     1681 ggccagcttg gccgggacga gaaaggtcac cgcgccgccc ggcgccacga gctccagcgc
     1741 gcgttcaacg aacgcgaccg acaggtctgc ctggtgcgag tagccggtgc cgccggcacc
     1801 cttccaccag cggtagcggc gcacaagctc ccggcgggtc ggtgggggga tcgcctcaga
     1861 gcgcacccac ggtgggttgc cgacgatcag gtcaaacccg ccgcgggcga acacatcggc
     1921 aaagtgggcc tggtaatgga accagggcag ctccccgtcc cggcggagcc gccgcattgc
     1981 gtccctcgtc gtggcccggt cgcttctcag tgccgccagg cgcgcgcgga ggtcgcgatc
     2041 cagtccccgt cgccgctcaa agagcgtggg cgcgcgcgca tcggacaggc attccgcgat
     2101 gccgtggtcg aactcgcgtt ccatggccgt gagcgtggcg cacgccgcgc gccgctcttc
     2161 ggtgcgcagc cggtcagtgg cgcggcgctt ctcgggaccg gtggctatca tggccagcga
     2221 gcgggccctc gccaatggct cggcgtcgaa cgccgggtct gcgccgtgag gcagagggtc
     2281 gcggagtgag tcgccctggc ggatggtgca gtcgaggttg gggagcggct ccacctcctc
     2341 gcccggcccg gccggatcgt cgcgcactac ggcgagccag agccggagtt cggccaggcg
     2401 caccgcggtg gcgctcaggt caacgccgaa gaggttcctc cgaagcacgg cccggcgggc
     2461 cgtggtgagc gcgcgcgcgg tgtcggcgtt ccgcctgtcg ctgagggtcg ctccgctgag
     2521 tcgttccaga gcgccgagga ggaacgcccc cgagcccgcg gcagggtcga ggaccgtgat
     2581 gtcggccagg aaccgccgtg ccgcggggtc cgggtccgcc agaagtcgga acgcccggtc
     2641 cgcgggaatg gccaactgtg cctggagcag ggcggcgagg ccggcatcca ccatccgcga
     2701 tacgagggtg ggtggcgtgt agaacgtccc ggtcaggcgg cgggtgtcgg gcgacatcag
     2761 cccctcgaac acccggccga gcatgtcggg ggctatcccg atggtgtccc cttccgtgac
     2821 ggtgaaatgg aaccgctcga agagctcgtc gaacacctgg cgccagacgg ccgtgggcgc
     2881 agccgcccgg acccgccgtt cgatcggatg cggttcgaag agccctccat tgaggaacgg
     2941 gatacggccg aaacgcagcg tcgactggct gcgccgcgcg acagggcggt tgagcgtacc
     3001 gaagaagagc gggtggagga ggtcgtgctg cggatgccgc ccgcgccgca ggcaatcgtc
     3061 gagcgagcgg gcgaggaagt cgctccgccc atccagccat cccttggcct ggacgaagta
     3121 caggaacagg atgcgggtca gctgcagcaa tgccagcgac tcgcggtcgg cgggaccggg
     3181 tccttcaagg gcctgcgcca gtcgatcgcg ccccagccgg aac
//
LOCUS       DASUAP010000265         8839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_634761, whole genome shotgun sequence.
ACCESSION   DASUAP010000265 DASUAP010000000
VERSION     DASUAP010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634761"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VFU45_06910"
     CDS             complement(<1..1102)
                     /locus_tag="VFU45_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5358829.1"
                     /translation="MPQELVSVTIDGRQVSVPKGTPIIEAAKQAGVLVPHYCYHPSLP
                     SPAVCRMCLVEVEKAPKLLPACVTTVAEGQVVHTQAARAKKAREGVLEFLLINHPLDC
                     PICDQSGECELQDYTFQEGRADGRYADYPKRYNPVEDFGPDILYVTNRCILCTRCVRF
                     MGDVAREPVLNVSERGDRAYIGIDPAHALTHPWSGNVVDLCPVGSLLSKDFLHKARAW
                     DLDKTASVCTGCSQGCNTMVETRDDVVVRLRPRPNLDVNRHFICDAGRLDYRWMNRGD
                     RVEAPLVPDGQKLVARDWETALERVAQLVTGATGAAVILASGRASNESLGEAMALLTG
                     RTVTAAVRVPLGEAEAPLAGVPGLAFRKERAPN"
     gene            complement(1108..2415)
                     /gene="nuoF"
                     /locus_tag="VFU45_06915"
     CDS             complement(1108..2415)
                     /gene="nuoF"
                     /locus_tag="VFU45_06915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU5358830.1"
                     /translation="MGYPNPSHAKETVVLSKHFGDAGARTFKGWVERGGYAGLRQALG
                     MTPEAVIDVIKASGLRGRGGAGFPTGLKWSFMPKNDGKPHYLVCNADESEPGTFKDRE
                     IMRWTPHALIEGCAVAAHAIGAEMCYIYIRGEFTEPWKVMTEAVKDAYANGALGDNVF
                     GTGRKIHVVLHRGAGAYICGEETALMNSIEGKRGNPRVKPPFPAAAGLFALPTTINNV
                     ETLTAAPHILVRGADWYKGLCRSNPKSTGTKLISVCGHVQRPGNYEITMGMPMKELIY
                     DLCGGMRPGRTLKAVIPGGSSVPIMDANDVETCLMDYEGVVAAGSMLGSGGMIVMDDT
                     ADIPYQVWRLARFYAHESCAQCTQCREGTAWTVKVLERILKGEGQESDLQLLLDLADN
                     MTGTTICVLSDSCAAPVVSGLKKFRGEFDAYLSRRRQPALASA"
     gene            complement(2415..2918)
                     /locus_tag="VFU45_06920"
     CDS             complement(2415..2918)
                     /locus_tag="VFU45_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU5358831.1"
                     /translation="MSASHGTAPYTPVFTGARRQELDQLFTKYPTRQACLLPALWMVQ
                     EDRGWIAPEAINEVAEVLGLTPAYVRGVVTFYTMYHMHPVGRHFIQVCTTSPCGLCGA
                     EDTLKALLRETGCRELGATSPDGRFTVVEAECLGACGFPTPILVDEDFIESVTPERVP
                     GVLQRYT"
     gene            complement(2915..4198)
                     /gene="nuoD"
                     /locus_tag="VFU45_06925"
     CDS             complement(2915..4198)
                     /gene="nuoD"
                     /locus_tag="VFU45_06925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEU5358832.1"
                     /translation="MAPRTVEFALATPVMDRHGRVSQAPLGAPLQGEDDLHGEHMLIN
                     IGPQHPATHGVLRLVIELDGERVIRVIPHIGYLHSGFEKLGEYRHYNQIVPLTDRTDY
                     LSPMANNVCLALAAEKLMGITVTERCQVLRVIACEMSRIISHLVWLGTTGIDLGAFTP
                     FLWSFQQRERIYDLQEAWTGARLTTSLTRVGGMMADVPEGWEEGLRDFCRTFPKTLRE
                     VDTMFTRNAIWIGRTQGVGVMSAADAVNYSLSGPMLRASGVPFDVRKDRPYLGYETYD
                     FDVPVGEHGDIYDRYRVRLEEMWQSTRILEQALDRLAALKGAPVNVADARVILPPKSQ
                     AMSDMESMIHHFKQVMEGVKAPPGEAYLSIENPKGELGYYFVSDGTAKPVRWRIRPPS
                     FINLAALPALCTGSLLSDVIAINASVDIVMGEIDR"
     gene            complement(4198..4809)
                     /locus_tag="VFU45_06930"
     CDS             complement(4198..4809)
                     /locus_tag="VFU45_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU5358833.1"
                     /translation="MTAHPSVAALRARFGAALARTSESCGDTIVWVTRDQAHSVLAWL
                     RDDPAQAYDYLVDVTAVEYRDLELPLEVVWQLRSLPHGRDLRVKVALDKALPLEVPSV
                     WDLWKGADWLEREVFDMFGVSFAGHPDLRRILMWETYHEGFPLRKDFPLRGHFSRAEQ
                     TRQALAANPEAHYSLEELSIAEAYDELPAEMRERLARGERGKV"
     gene            complement(4806..5426)
                     /gene="nuoB"
                     /locus_tag="VFU45_06935"
     CDS             complement(4806..5426)
                     /gene="nuoB"
                     /locus_tag="VFU45_06935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEU5358834.1"
                     /translation="MGLTLPSAPSGTTTMSEMSPNWVTTRLDFLVNWARKNSLWPMPF
                     GTACCAIEYMATAASRFDIARFGMERQSFSPRQADVLICAGRLPFKLAPVIRKIWDQM
                     PQPKWAISMGACASSGGIFDTYAMVQGIDTIIPVDVYVPGCPPRPEGLIYGITLLQKK
                     IAGETIADHAMRDEHLTGPDGLFVRPEAIDVVSEPFGNSVQQTRSA"
     gene            complement(5417..5794)
                     /gene="ndhC"
                     /locus_tag="VFU45_06940"
     CDS             complement(5417..5794)
                     /gene="ndhC"
                     /locus_tag="VFU45_06940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5358835.1"
                     /translation="MLQSYLPILLVIAFVIANAVLMLGVSRLSDLVLRTYQPTAVKVA
                     PYESGMPVLGDARERISVKFYMVTILFIVFDIEAVFMIPWAVAFQQLAAMRGLLILEM
                     LAFMLILVVGFVYVWQRGALEWD"
     gene            5989..6567
                     /gene="dcd"
                     /locus_tag="VFU45_06945"
     CDS             5989..6567
                     /gene="dcd"
                     /locus_tag="VFU45_06945"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEU5358836.1"
                     /translation="MSIKSDRWIRRMARERGMIEPFVEGQVREVDTQGRKVISYGVSS
                     YGYDMRVAPEFKIFTNVLSAIVDPKDFDPKSFVEFSGAVCIVPPNSFALARSVEYFRI
                     PREVLTICVGKSTYARCGIITNVTPFEPEWEGHVTLEISNTTPLPAKIYANEGICQVL
                     FFEADAEDVCETSYKDKAGKYQGQLGVTLPRL"
     gene            6708..8762
                     /locus_tag="VFU45_06950"
     CDS             6708..8762
                     /locus_tag="VFU45_06950"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5358837.1"
                     /translation="MRKLLIPLLFAALPAALGAQQPTAPTAPPPPVVARPQQPAQAPL
                     RTPTADTGIFAHLDLTPGNEVRLPDGRPGPKYWEQRADYDIVATLDTAAKTITGTVRI
                     TYTNHSPAALDHVFLQLDQNLFRQGSVGSLMFPPNSRFGTRGFDGGFTLASITQESPV
                     RGRAKAKSVAKKLTGRTDDTMLFLPLADPLAPGQSTVIAIDYSFNIPEHGADRMGYDG
                     PLFELAQWYPRMAVYDDVRGWNTDQYLGQGEFYLEYGDFTYAVTVPAGYIVAGTGTVQ
                     NPAEVYTATERARLAAAAKSDTTIHIVTEADLASGAARPKKDGMVTWRFKAQNVRDVA
                     WAGSPEYLWDASSYDGHYAFAYYRATAKSTWADAAKMSRFSIKEYSERWLPYPYPHIS
                     AVEGPISGMEYPMVAMEAPNTPRAAGAEALQEGKESLYNVITHEIGHMWYPMIVGSNE
                     RLYAWMDEGFNTFINTFSEEDYWQRNDSTNRKGEEKFFVMVNDQRPTAQPIMTPANRY
                     RTNANLGELAYVKPSIALLALRNVVLGPEVFDKAFSEYTHRWAFKHPTPADFFRTMED
                     MSGRDLSWFWREWFYTTATLDQGIDNVTQTANADGSSDVKVALKNNGAAVMPVDLKLT
                     LSDGSVQMVKLPVEVWYYDQTKFTSEIRTAKPVVKAELWTSGWFKDYDSSNDTWTAPA
                     GN"
BASE COUNT         1431 a         3052 c         3013 g         1343 t
ORIGIN      
        1 cgttgggggc ccgctccttc cggaacgcga ggccgggcac gccggcgagc ggcgcttcgg
       61 cctcgcccag cgggacgcgg accgccgcgg tcacggtgcg gcccgtgagc agcgccatcg
      121 cctcgcccag ggactcgttg gaggcgcgtc ccgacgcgag gatcaccgcc gcgcccgtgg
      181 cacccgtcac caactgggcg acgcgctcca gcgccgtctc ccagtcccgc gcgacgagct
      241 tctggccatc cggcaccagc ggcgcctcga cccggtcgcc gcggttcatc cagcgatagt
      301 ccagccggcc cgcgtcacag atgaagtggc ggttgacgtc gaggttggga cgcggccgca
      361 gccggaccac cacgtcgtcg cgggtctcga ccatcgtgtt gcagccctgg ctgcacccgg
      421 tgcagacgct ggcggtcttg tcgaggtccc aggcgcgcgc cttgtgcagg aaatccttgg
      481 agaggagcga gcccaccggg cagaggtcca cgacgttgcc ggaccacggg tgggtcagcg
      541 cgtgggcggg gtcgatgccg atgtaggcgc ggtcgccgcg ctccgacacg ttgaggaccg
      601 gctcccgggc cacgtcgccc atgaagcgga cgcaccgggt acagaggatg cagcggttgg
      661 tgacgtacag gatgtccggc ccgaagtcct cgaccgggtt gtagcgcttg ggatagtcgg
      721 cgtaccgtcc gtcggcgcgc ccttcctgga acgtgtagtc ctgcagctcg cattcacccg
      781 actgatcgca gatcgggcaa tcgagcggat ggttgatgag caggaactcg agcacgccct
      841 cgcgcgcctt cttggcgcgg gcggcctggg tatgcaccac ctggccctcg gccacggtgg
      901 tgacgcaggc cggcaggagc ttgggcgcct tctccacctc caccaggcac atccggcaga
      961 cggcaggact gggcagcgac gggtgatagc agtagtgcgg caccagcacg cccgcctgct
     1021 tggcggcttc gatgatcggc gtccccttcg ggacgctcac ctggcggcca tcgatcgtga
     1081 cggagacgag ctcctggggc atggcgctca cgcggaggcg agcgccggct ggcgccggcg
     1141 gctcaggtag gcgtcgaatt ctccccggaa cttcttcagc ccgctcacca ccggggccgc
     1201 gcacgagtcg gagaggacgc agatggtcgt gccggtcatg ttgtcggcca ggtcgagcag
     1261 gagctggagg tcgctctcct gcccctcccc cttgaggatc cgctccagga ccttgacggt
     1321 ccaggcggtg ccctcgcggc actgggtgca ctgggcgcac gactcgtggg cgtagaagcg
     1381 ggccaggcgc cacacctggt aggggatgtc ggcggtgtcg tccatcacga tcatcccgcc
     1441 ggacccgagc atcgagcccg cggccacgac cccctcatag tccatcaggc acgtctcgac
     1501 gtcattggcg tccatgatcg gcaccgacga cccgcccgga atgaccgcct tgagcgtccg
     1561 ccccgggcgc atcccgccgc acaggtcgta gatgagctcc ttcatcggca tgcccatcgt
     1621 gatctcgtag ttccccggcc gctgcacgtg gccgcacacc gagatgagct tggtgcccgt
     1681 gctcttgggg ttgctccggc agaggccctt gtaccagtca gcgccgcgga cgaggatgtg
     1741 cggcgcggcg gtgagggtct ccacgttgtt gatggtggtg ggcagcgcaa agagccccgc
     1801 ggcggccggg aacggtggct tgacccgcgg attgccccgc ttcccctcga tggagttcat
     1861 cagggcggtc tcttcgccgc agatgtaggc gcccgcgccg cggtggagca cgacgtggat
     1921 cttccggccg gtgccgaaca cgttgtcgcc cagggcgccg ttggcgtagg cgtccttgac
     1981 cgcctcggtc atcaccttcc acggctcggt gaactcgccc cggatgtaga tgtagcacat
     2041 ctcggccccg atggcgtgcg ccgccacggc gcacccctcg atgagcgcgt gcggggtcca
     2101 gcgcatgatc tcccggtcct tgaaggtccc gggttcggat tcatcggcgt tgcagacgag
     2161 gtagtgcggc ttgccgtcgt tcttcggcat gaacgaccac ttgaggccgg tcgggaaccc
     2221 cgcgccgccg cgcccgcgca accccgacgc cttgatgacg tcgatgacgg cctcgggggt
     2281 catccccagg gcctgccgga gccccgcgta cccgccgcgc tcgacccagc ccttgaaggt
     2341 ccgggcgccc gcgtcgccga aatgcttgga gaggaccacc gtctccttgg cgtggctcgg
     2401 attcgggtag cccatcaggt gtacctctgg agcacgcccg gcacccgctc tggcgtgacg
     2461 ctctcgatga agtcctcgtc caccaggatg ggcgtgggga agccgcacgc gccgaggcac
     2521 tccgcctcca cgacggtgaa ccggccgtcg gggctggtgg cgccgagctc ccggcagccg
     2581 gtctcgcgca ggagcgcctt gagcgtgtcc tcggcgccgc agagcccgca ggggctcgtg
     2641 gtgcacacct ggatgaagtg ccggcccacc ggatgcatgt ggtacatggt gtagaaggtc
     2701 accacgccgc ggacatacgc cggggtgagc cccaggacct ccgccacctc gttgatggcc
     2761 tccggggcga tccagccgcg gtcctcctgc accatccaga gcgcggggag gaggcacgcc
     2821 tgcctggtcg ggtacttcgt gaacagctgg tccagctcct gccgccgggc gccggtgaag
     2881 accggcgtgt acggggccgt gccgtgcgac gcgctcaccg gtcgatctcc cccatcacga
     2941 tgtccacgct cgcgttgatc gcgatcacgt ccgacaggag cgacccggtg cagagcgccg
     3001 ggagggccgc caggttgatg aaggacggcg gacggatccg ccaccgcacc ggcttggcgg
     3061 tcccgtcgga gacgaagtag tagccgagct cccccttggg gttctcgatc gacaggtagg
     3121 cctcgcccgg gggcgccttc acgccttcca tcacctgctt gaagtgatgg atcatcgatt
     3181 ccatgtcgct catcgcctgc gacttgggcg ggaggatgac gcgggcgtcg gccacgttca
     3241 ccggcgcgcc cttgagcgcc gccagccggt cgagcgcctg ctccaggatc cgggtcgact
     3301 gccacatctc ctcgagccgc acgcggtagc ggtcgtagat gtcgccgtgc tcaccgaccg
     3361 gcacgtcgaa atcgtacgtc tcatagccga gatagggccg gtccttccgc acgtcgaacg
     3421 gcactccgct cgcgcggagc atcgggccgg agagcgagta gttgacggcg tccgccgccg
     3481 acatgacccc gacgccctgg gtccgcccga tccagatggc gttgcgggtg aacatcgtgt
     3541 ccacctcgcg gagcgtcttc gggaaggtcc ggcagaagtc gcggagcccc tcttcccacc
     3601 cctccggcac gtcggccatc atcccgccga cccgggtgag cgaggtggtg agccgggccc
     3661 cggtccacgc ctcctgcagg tcgtagatcc gctcgcgctg ctggaaggac cagaggaacg
     3721 gcgtgaaggc gccgagatcg atcccggtgg tgccgagcca gaccaggtgg ctgatgatcc
     3781 ggctcatttc gcaggcgatc acccggagca cctggcagcg ctcggtgacg gtgatcccca
     3841 tcagcttctc ggccgccagc gccaggcaga cgttgttggc catcggcgag aggtagtcgg
     3901 tccggtcggt gagcggcacg atctggttgt agtgccggta ttcgccgagc ttctcgaagc
     3961 cggagtgcag gtagccgatg tgggggatga cgcggatcac ccgctcgccg tcgagctcga
     4021 tgacgaggcg gagcacgccg tgcgtggcgg ggtgctgagg gccgatgttg atcagcatgt
     4081 gctcgccgtg caggtcgtcc tcgccctgca ggggcgcgcc cagcggcgcc tgggagaccc
     4141 gcccgtgccg gtccatcacg ggggtggcga gcgcgaactc gacggtgcgt ggggccatca
     4201 gaccttcccc cgttcgccgc gcgccagccg ctcccgcatc tcggcgggca gctcgtcgta
     4261 cgcctcggcg atggagagtt cctcgagcga gtagtgggcc tcgggattgg cggcgagcgc
     4321 ctggcgggtc tgctcggcgc ggctgaagtg gccgcggagc gggaagtcct tccggagcgg
     4381 gaaaccctcg tggtaggtct cccacatcag gatgcggcgg aggtcggggt ggccggcgaa
     4441 ggagacgccg aacatgtcga agacctcgcg ctccagccag tcggcgccct tccagaggtc
     4501 ccacaccgac ggcacctcga gcgggagcgc cttgtcgaga gcgaccttga cccggaggtc
     4561 ccggccgtgc gggagcgacc gcagctgcca gacgacctcg agcgggagct ccaggtcgcg
     4621 gtactcgacg gcggtgacgt ccacgaggta gtcgtatgcc tgcgccggat cgtcccgcag
     4681 ccaggcgagg accgagtggg cctggtcgcg ggtcacccag acgatcgtat cgccgcagct
     4741 ctcgctcgtg cgggccagcg cagcgccgaa gcgggcccgg agggccgcca cgctcgggtg
     4801 ggcggtcatg cggagcgggt ctgctggacc gagttgccga agggctccga caccacatcg
     4861 atggcctcgg gacggacgaa caggccgtcc gggccggtca ggtgctcgtc ccgcatcgcg
     4921 tggtcggcga tcgtctcgcc ggcgatcttc ttctggagga gcgtgatccc gtagatcagg
     4981 ccttccgggc gcggcgggca ccccggcacg tagacatcca ccgggatgat cgtgtcgatc
     5041 ccctgcacca tcgcgtaggt gtcgaagatg ccgcccgagg aggcgcaggc gcccatcgag
     5101 atggcccact tcggctgcgg catctggtcc cagatcttgc ggatgaccgg cgcgagcttg
     5161 aacggcaggc gcccggcgca gatgagcacg tccgcctggc ggggggagaa gctctggcgc
     5221 tccatcccga agcgggcgat gtcgaagcgc gaggccgcgg tggccatgta ctcgatggcg
     5281 cagcaggcgg tgccgaaggg catcggccag agggagttct tgcgggccca gttgaccagg
     5341 aaatcgagcc gggtggtcac ccagttgggc gacatctccg acatcgtggt ggtcccggac
     5401 ggggccgagg gcagggtcaa tcccattcga gcgctccccg ttgccacaca tagacgaagc
     5461 ccaccacgag gatgagcatg aaggccagca tctccaggat cagcagcccg cgcatggcgg
     5521 cgagctgctg gaaggccacg gcccacggga tcatgaacac cgcctcgatg tcaaagacga
     5581 tgaacaggat ggtgaccatg tagaacttga ccgagatgcg ttcccgcgca tcgcccagga
     5641 ccggcatccc cgactcatac ggggcgacct tgaccgcggt gggctggtag gtgcggagga
     5701 cgaggtccga caagcgcgac acgccgagca tcaggaccgc gttggcgatc acgaaggcga
     5761 tgaccagcag gatcggcagg taggactgga gcatgggctc ggcgtatcgt gaaatttttc
     5821 acttacggaa agatgaagct aatccccgga ctgggcgagt tcaacccgca ccgcgccgga
     5881 catggccgcg cattcccgac cgccatcgct gggattcagc acaccgccgc cccggggtat
     5941 attccgcacc ggtcgcgccg gtccccgtcc ccccatcccc gctcccccat gtccatcaag
     6001 tccgaccgct ggatccgccg gatggcccgc gagcgcggga tgatcgagcc gttcgtcgag
     6061 ggccaggtcc gcgaggtgga cacccagggg cggaaggtca tctcctacgg ggtgtcgtcc
     6121 tacggctatg acatgcgggt ggcgccggag ttcaagatct tcaccaacgt gctgtcggcc
     6181 atcgtggacc cgaaggactt cgacccgaag agcttcgtcg agttctcggg tgcggtctgc
     6241 atcgtgccac ccaacagctt tgccctggcg cggtcggtgg agtacttccg catcccccgc
     6301 gaggtgctga cgatctgcgt cggcaagtcg acctacgccc gctgcggcat catcaccaac
     6361 gtcacgccgt tcgaaccgga atgggaaggg cacgtgacgc tcgagatctc caacaccacg
     6421 ccgctgcccg ccaagatcta cgccaacgag ggcatctgcc aggtgctgtt cttcgaggcc
     6481 gatgccgagg acgtctgcga gaccagctac aaggacaagg ccggtaagta ccagggccag
     6541 ctgggcgtga cacttccgcg gctgtaaggc cgtagacggt gaacggtgcg cggtcaacag
     6601 catacgggcg gtcaccccgt cgaatgacgc gcgccgtgtt caccatccac cgttgaccga
     6661 tcaccgttga gcgttgaccc ctttcccaat accccgagcc cacgcacatg cgcaaactcc
     6721 tgatccccct gctcttcgcc gcgctccccg cggcgctggg cgcccagcag ccgaccgcgc
     6781 cgacggcgcc gccgccgccc gtcgtggccc gaccgcagca gcccgcccag gcccccctgc
     6841 ggaccccgac cgcggacacc ggcatcttcg cgcacctgga cctgacgccg ggcaacgagg
     6901 tccggttgcc cgacggccgc cccggcccca agtactggga gcagcgcgcg gactacgaca
     6961 tcgtcgccac cctcgacacc gcggcgaaga cgatcacggg gacggtgcgc atcacctaca
     7021 ccaaccactc cccggccgcg ctcgaccacg tcttcctgca gctcgaccag aacctgttcc
     7081 gccaggggag cgtcggctcg ctgatgttcc cgcccaactc ccggttcggc acccgcggct
     7141 tcgacggcgg cttcacgctg gcctcgatca cccaggagtc gccggtccgg ggccgggcca
     7201 aggcgaagtc cgtggccaag aagctcaccg gccgcaccga cgacacgatg ctgttcctcc
     7261 cgctcgccga tccgctcgca ccgggacagt cgacggtcat cgccatcgac tactcgttca
     7321 acatccccga gcacggcgcc gaccggatgg gctatgacgg cccgctcttc gagctggccc
     7381 agtggtaccc ccggatggcg gtgtacgatg acgtccgcgg ctggaacacc gaccagtacc
     7441 tgggccaggg cgagttctac ctcgagtacg gcgacttcac ctacgcggtg accgtcccgg
     7501 cgggctacat cgtggcgggc accggcacgg tgcagaaccc cgccgaggtg tacaccgcca
     7561 cggagcgcgc ccggctggcc gcggcggcga agagcgacac cacgatccac atcgtgaccg
     7621 aggccgacct cgcgagcggg gccgcccggc ccaagaagga cgggatggtc acctggcgct
     7681 tcaaggccca gaacgtgcgc gacgtggcgt gggccggctc gcccgagtac ctgtgggacg
     7741 cgtcgagcta cgacggccac tatgccttcg cgtactaccg ggccacggcc aagtcgacct
     7801 gggccgacgc cgccaagatg agccggttct cgatcaagga gtacagcgag cggtggcttc
     7861 cctaccccta cccgcacatc tccgccgtcg agggcccgat cagcgggatg gagtacccga
     7921 tggtcgcgat ggaggcgccc aacacgccgc gcgccgccgg cgccgaggcg cttcaggagg
     7981 gcaaggagtc gctctacaac gtgatcacgc acgagatcgg gcatatgtgg tacccgatga
     8041 tcgtcggatc gaacgagcgg ctctacgcct ggatggacga ggggttcaac acgttcatca
     8101 acaccttctc cgaagaggac tactggcagc ggaatgactc caccaaccgc aagggggagg
     8161 agaagttctt cgtgatggtc aacgaccagc ggccgacggc ccagcccatc atgacgccgg
     8221 ccaaccgcta ccgcaccaac gccaacctgg gcgagctggc gtacgtcaag ccgtcgatcg
     8281 cgctcctcgc cctgcggaac gtggtgctcg ggcccgaagt gttcgacaag gcgttcagcg
     8341 agtacaccca ccgctgggcg ttcaagcacc cgaccccggc cgacttcttc cgtacgatgg
     8401 aggacatgtc cggccgtgac ctgagctggt tctggcggga gtggttctac accaccgcca
     8461 cgctcgacca gggcatcgac aacgtgaccc agaccgccaa tgccgacggc tcgagcgacg
     8521 tgaaggtcgc gctcaagaac aacggcgcgg cggtgatgcc ggtggacctc aagctcaccc
     8581 tgagcgatgg gtcggtgcag atggtcaagc tgccggtcga ggtgtggtac tatgaccaga
     8641 ccaagttcac gagcgagatc cgcaccgcga agcccgtggt gaaggccgag ctctggacca
     8701 gcggctggtt caaggactac gactcgtcca acgacacctg gacggcgccc gccggcaact
     8761 gagcggcggc ggccgaacga cgaacggcgg gctccgggat ccccggggcc cgccgttcgc
     8821 tttccgcctc cgcggcgcg
//
LOCUS       DASUAP010000266         7554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_879995, whole genome shotgun sequence.
ACCESSION   DASUAP010000266 DASUAP010000000
VERSION     DASUAP010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879995"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VFU45_06955"
     CDS             complement(<1..1456)
                     /locus_tag="VFU45_06955"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU5358838.1"
                     /translation="MTDQLDTLLHEQRRFPPPPEFAAKALADHNFVASAADAERFWAA
                     QAAELDWMRPWDRVLEWTPPHAKWFQGGKLNASANCLDRHLTGPRRNKAALIWEGEPG
                     DRRVLTYWDLAREVGRAANALKRLGVKRGDRVAIYLPMVPEAAIAMLACARIGAVHSV
                     VFGGFSADSLRDRINDAGANLLITADGGYRRGQVVPLKRFADDALTGCPTIRHVVVVR
                     RRAGADGDEAFANMTEGRDHWWHRLLDAESPDCPPEAMDAEDLLYILYTSGTTGKPKG
                     IVHTTGGYLTHVASTTRYVFDLKDEDVFWCTADVGWVTGHSYVVYGPLAIGATVVMYE
                     GAPDWPERDRFWKLVARYGVTVLYTAPTAIRAFMKWGEALPARHDLSTLRLLGSVGEP
                     INPEAWMWYHQHIGGGRCPIVDTWWQTETGAIAISPLPGITETKPGSATVPVPGFTAE
                     LLDAKGNVIPVGGGLLALTKPWPSMLRTIWGDDER"
     regulatory      1745..1839
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1942..2253
                     /locus_tag="VFU45_06960"
     CDS             1942..2253
                     /locus_tag="VFU45_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5358839.1"
                     /translation="METALMQETLMAPEHLGLQSRTDFRQQAVTLLERLPEDGGRLVV
                     DLSATRQVDSAGLGALMLIQRKAAERRQVVVLRDANEELRFLLVLTKLEDLFTIEPPP
                     A"
     gene            2294..2659
                     /locus_tag="VFU45_06965"
     CDS             2294..2659
                     /locus_tag="VFU45_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5358840.1"
                     /translation="MTPPAPRRRVLFVEDEPALRTSYERFFRDRYAMQFASSGSAALA
                     AIKATPPEVMILDMRLPDTDGLALLREVRREHPTLPVIVTTAYVSVEPQLRMLDLQVS
                     GYLVKPFDLSDLGSSIDAA"
     gene            2649..3356
                     /gene="ccmA"
                     /locus_tag="VFU45_06970"
     CDS             2649..3356
                     /gene="ccmA"
                     /locus_tag="VFU45_06970"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEU5358841.1"
                     /translation="MQPDAPVLEVRGLTRRYGRARVLKGVDLTVRAGEVVALLGPNGA
                     GKTTLLRVLAGLARAEDGVVLVRGRHPAADPLAARRAIGLVEHRSLLYGELTPRENLA
                     FTARLFGLDGAAVAAAVERLDIGKHADLPVASLSRGMVQRTSVARALLHAPSILLLDE
                     PFSGLDAPSSARLVSLLSARRAEGVAVLLVTHQPEEAWSLITRVAVLHGGRVVTDVAR
                     PSTPAETSRLYHEAIGG"
     gene            3349..4029
                     /locus_tag="VFU45_06975"
     CDS             3349..4029
                     /locus_tag="VFU45_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU5358842.1"
                     /translation="MGDAIRVALAVAGKDLQLELRRRTALLAATVFAALVLVVFNFAR
                     DPTALSALDLAPSALWITLVFSSEVALNRAFHAEAEHGALDGLLLAPASRGGLYLGKC
                     LANLIFVGVVLLVAVPLFILFFNLDPSTGWTGVAAVLVLAAVGIVAVGTLFSAMAVRT
                     RFAELLLPLLHLPFVVPILIAGVQASTRLLAGRPLSEVGGWLRFLLVYDIVFLTLGSL
                     AFSTVVDE"
     gene            4026..4721
                     /gene="ccsA"
                     /locus_tag="VFU45_06980"
     CDS             4026..4721
                     /gene="ccsA"
                     /locus_tag="VFU45_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU5358843.1"
                     /translation="MSDPMARPRAMVRWGAAITATALALLVGVVVRALLYTPVEVHQG
                     PAQKIFYMHAPAAWSAMLAFSLVGIVSAAYLIMKDERLDRFAACSAEVGLAFSGVMLT
                     TGPIWGKTVWGTWWTWDARLTLTLFLFFLFLGYLALRAAVNDPGERARFAAVVGILGL
                     VLVPFIHLSVYLFRTLHPQPVVLKPSAPSLPSDMLITLLTSFGVFTLLYIGFVTWRYG
                     IALAADLAGGDDD"
     gene            complement(5095..5772)
                     /locus_tag="VFU45_06985"
     CDS             complement(5095..5772)
                     /locus_tag="VFU45_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU5358844.1"
                     /translation="MRSLARPLFVEALGTFTLVFVGVAAMMANNYPLVGFNMLGIALA
                     HGLALGLAITIALPYSGGHVNPAVTLGFLSVGRIDLKKALWYIAAQLLGGVLGALAVK
                     ATYPGGVTSVTDLGVPTIHSAISFAGAIGIEAILTFFLMTAVYATIVSPKATRMGGFG
                     VGLTVFVMILAGGPLTGAIMNPARAFGPSLVTMQWTGHLAYWIGPVLGAIVAAMVWDK
                     FLLKGEK"
     gene            complement(5928..7178)
                     /gene="fabF"
                     /locus_tag="VFU45_06990"
     CDS             complement(5928..7178)
                     /gene="fabF"
                     /locus_tag="VFU45_06990"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5358845.1"
                     /translation="MAEPRRVVITGIGAVTPIGTGAEPLWAGLRARRSAVGPVTRFDA
                     TPFRTRVAGEVRDFHPSDFVEERRVRRMDRCSQFTLAAARLALSDAALDLRREDSDRT
                     GAMMGTALGGVAHAEEQHARYLRGGVRDVDPGLALTVFAGAASCNLAIEFGLTGPNAT
                     NGMSCASGAMAIGDAYRTIRRGEADVMLAGGAEAPLAPLSFGAFSIIRAMSTRNDDPA
                     AASRPFDRGRDGFVMAEGSAVLVLEERGRALARGAPVYAEVCGYGTTNDAHHMTAPRP
                     DGAQAARAITLALADAHVPAASVGYVNAHGSATALNDPTETLAIRRAFGPHATALAVS
                     GTKGYYGHALGASGAFEAAICALASRRRWLPPTVNLDSPDTACDLAYLRGDGVDADPE
                     YLLSNSFGFGGINAALLFRRADGR"
     gene            complement(7171..>7554)
                     /locus_tag="VFU45_06995"
     CDS             complement(7171..>7554)
                     /locus_tag="VFU45_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358846.1"
                     /translation="EWLPHYRWVRLTPGGEGGAATVEMAAWRPFGSLNWPTWWTSEMW
                     IDRSRPAVRYRHIRGITRGMDVEWSFSSEGEGTVVTIVHEWTGPRWPLIGRAAAAWII
                     GPVFVHGIASRTLAGIARVVERADG"
BASE COUNT         1084 a         2617 c         2704 g         1149 t
ORIGIN      
        1 agcgctcatc gtcgccccag atggtgcgca gcatcgaggg ccacggcttg gtgagcgcga
       61 gcagtccacc gccgacgggg atcacgttgc ccttggcgtc gaggagctcg gcggtgaagc
      121 cgggtaccgg gaccgtggcg ctgccgggct tcgtctcggt gattccggga agcggtgaga
      181 tcgcaatcgc gccggtctcc gtctgccacc aggtgtcgac gatggggcag cgcccgccgc
      241 cgatgtgctg gtggtaccac atccacgcct ccgggttgat gggctcgccc accgagccga
      301 ggagccggag cgtcgagagg tcgtggcggg cggggagcgc ctcaccccac ttcatgaagg
      361 cgcggatggc ggtcggcgcg gtgtagagca ccgtcacgcc gtagcgcgcc accagcttcc
      421 agaagcggtc ccgttcgggc cagtcggggg caccctcgta catcaccacc gtggcgccga
      481 tggcgagcgg cccgtacacc acataggagt ggcccgtcac ccagccgacg tcggcggtgc
      541 accagaagac gtcctcgtcc ttgaggtcga agacgtagcg tgtcgtggac gccacgtggg
      601 tcaggtaccc gccggtggtg tggacgatgc ccttgggctt cccggtggtc cccgaggtat
      661 agaggatgta cagcaggtcc tcggcgtcca tcgcctcggg cgggcagtcg ggcgattcgg
      721 catcgagcaa ccggtgccac cagtggtcgc ggccctcggt catgttggcg aaggcctcgt
      781 cgccgtcggc cccggcgcgg cggcgcacca ccacgacgtg ccggatcgtg gggcagccgg
      841 tcagcgcgtc gtccgcgaag cgcttgagcg gcaccacctg cccccgccga tacccgccgt
      901 cggcggtgat cagcaggttg gcgccggcgt cgttgatgcg gtcccggagc gaatcggcgg
      961 agaagccgcc gaacaccacg gagtgcaccg cgccgatccg cgcgcacgcg agcatcgcga
     1021 tggccgcctc cggcaccatc ggcaggtaga tggcgacgcg gtcgccgcgc ttgacgccga
     1081 gccgtttgag ggcgttggcg gcccgcccca cctcgcgcgc caggtcccag taggtgagga
     1141 cccgccggtc gccgggttcc ccttcccaga tgagcgcggc cttgttgcgc cgcgggccgg
     1201 tgagatgccg gtcgaggcag ttggcggagg cgttgagctt cccgccctgg aaccacttcg
     1261 cgtggggcgg ggtccattcg aggacccgat cccacggccg catccagtcg agctccgccg
     1321 cctgggcggc ccagaatcgc tcggcatcgg cggcggacgc gacgaagttg tggtcggcca
     1381 gcgccttcgc ggcgaactcc ggcggaggcg ggaagcggcg ctgttcgtgg agcagggtat
     1441 cgagttggtc ggtcatatcc agggatcggc aagcaggaag agggggtgac gcccatccat
     1501 cttagccacc gaaaccgggt cgtgtaagcg acgccctggc cccgccgggt gtcaagttcc
     1561 tcgacgatgt caaagaattg aggggttgca ggacccggca acttcacgct cggcgcaacg
     1621 aaaatcaggg cgcgaccggg gtcacactgg tggcataact cgttgtaaca gcgccacttg
     1681 aatacttggg caccgacttc gccttagaag gggccggcgc caccaggcgc cagacaactg
     1741 aaccgcccga taagagcaca cccggagcga tccggggccg caaaacttga ggtctcgtgt
     1801 ccggcgaatg ccggcgccac gagatagctc ggtgccgaag gtggagcaga cgatgcgcat
     1861 ccgcgcggag actctcctgt tgacccatcg gcggcgatgg cagggagggt ctcctgccat
     1921 tgtcgggcgg aggagcccgt aatggagacc gcgctgatgc aagagaccct gatggcccct
     1981 gagcacttgg gtctgcaaag ccggacggat ttccgccagc aggccgtgac gctgctcgag
     2041 cgcctgcctg aggacggggg acgtctggtg gtggacctca gcgccacgcg gcaggtcgat
     2101 tccgccggac tgggcgcgct gatgctgatc cagcgcaagg cggcggagcg acgccaggtg
     2161 gtggtgctcc gcgatgcgaa cgaggagctt cgcttcctgc tggtgctcac gaagctcgag
     2221 gacctgttca ccatcgagcc gccgccggcc tgaccggccc cggggccggg ccggggccgg
     2281 gtatattccg gcaatgaccc cgcccgcccc gcgccgacgc gtcctgttcg tcgaggatga
     2341 accggcgctc cggaccagct acgagcggtt cttccgtgac cgatatgcga tgcagttcgc
     2401 atcgtccggg agcgccgccc tggccgccat caaggccacc ccgcccgaag tgatgatcct
     2461 cgacatgcgc ctccccgaca ccgacggcct ggcgctcctg cgcgaggtcc ggcgcgagca
     2521 cccgacgctg ccggtgatcg tcacgacggc gtacgtgagc gtggaaccgc agctccggat
     2581 gctggacctc caggtgagcg ggtacctggt caagccgttc gacctctccg acctgggcag
     2641 ctccatcgat gcagcctgac gccccggtgc tcgaggtacg cgggctcacc cgccgttacg
     2701 gccgcgcccg ggtgctcaag ggggtggacc tcacggtccg ggccggcgag gtggtggcgc
     2761 tcctcggccc caacggtgcg gggaagacca cgctcctgcg ggtgctggcc ggcctggccc
     2821 gcgccgagga cggcgtggtg ctggtgcggg gccgccatcc ggccgccgat ccgctggccg
     2881 cgcgccgcgc catcgggctg gtggaacacc gctcgctcct ctacggcgag ctgacgccga
     2941 gggagaacct cgccttcacc gcccggctct ttggcctcga cggcgcggcc gtggcggcgg
     3001 cggtggagcg gctcgacatc ggcaagcacg ccgacctgcc ggtggcgtcg ctgagccgtg
     3061 ggatggtcca gcggacctcg gtggcgcggg cgctccttca tgcgccgtcg atcctgctcc
     3121 tcgacgagcc gttcagcggc ctggacgcgc cgtcgtcggc gcggctggtc tcgctcctca
     3181 gcgcgcgtcg cgccgagggg gtcgcggtgc tcctggtgac gcaccagccc gaggaggcgt
     3241 ggtcgctgat cacgcgggtg gcggtgctcc acggcgggcg cgtcgtcacc gacgtggccc
     3301 ggccctccac gccggcggag acgtcccggc tctaccacga ggccatcggt gggtgacgcg
     3361 atccgggtgg cgctcgcggt ggccgggaag gacctgcagc tcgagctgcg gcgccgcacg
     3421 gcgctcctcg cggcgacggt ctttgcggcg ctcgtgctgg tggtgttcaa cttcgcccgc
     3481 gacccgaccg cgctctcggc cctcgacctg gcaccgagcg cgctctggat cacgctggtg
     3541 ttctcgtccg aggtggcgct caaccgcgcc ttccacgccg aggccgagca cggcgccctc
     3601 gacggcctgc tgctggcacc ggcctcgcgc ggcggcctct acctcggcaa atgcctggcc
     3661 aacctgatct tcgtgggcgt ggtgctgctg gtggccgtgc cgctcttcat cctgttcttc
     3721 aacctggacc cgtcgaccgg gtggaccggc gtggcggcgg tgctggtgct cgcggcggtg
     3781 gggatcgtgg ccgtggggac gctcttcagc gccatggcgg tgcgcacccg cttcgcggag
     3841 ctcctgctgc cgctcctcca cctcccgttc gtggtgccga tcctgatcgc cggggtgcag
     3901 gcctcgaccc ggctgctggc cgggcgtccg ttgagcgagg tgggggggtg gcttaggttc
     3961 ctcctcgtgt acgacatcgt cttcctgacg ctgggatccc tggccttttc cacggtggtg
     4021 gacgaatgag cgatccgatg gcgcggccgc gcgcgatggt gcgctggggg gccgccatca
     4081 cggcgaccgc cctggcgctg ctcgtcgggg tggtcgtgcg ggccctgctc tacacgccgg
     4141 tcgaggtgca ccagggcccg gcgcagaaga tcttctacat gcacgcgccc gccgcctggt
     4201 cggcgatgct cgccttctcg ctcgtcggca tcgtgagcgc ggcctacctc atcatgaagg
     4261 acgagcggct cgaccgcttt gccgcctgct cggccgaggt gggcctggcc ttcagcggcg
     4321 tgatgctcac caccggtccc atctggggca agacggtctg gggcacctgg tggacgtggg
     4381 atgcccggct caccctcacg ctcttcctct tcttcctctt cctcggctac ctcgcgctcc
     4441 gcgccgcggt gaacgacccg ggtgagcggg cccgcttcgc ggcggtggtc gggatcctgg
     4501 gtctcgtgct ggtcccgttc atccacctga gcgtctacct ctttcggacg ctccacccgc
     4561 agcccgtcgt gctcaagccg agcgcgccgt cgctgccgag cgacatgctc atcaccctgc
     4621 tcacgtcgtt cggggtgttc accctgctct atatcggctt cgtcacctgg cgctacggga
     4681 tcgcactcgc cgccgacctg gccggagggg atgatgactg aactgacgcc gcagaacggc
     4741 gccttcatgg tggcgggcta cgtggtgacc ggcgtgatcc tgctgggcta cacgctgtcg
     4801 ctctgggcgc ggagcgggaa agcaaggaag taagaaggag agaggagaag gagagaggag
     4861 aaggaagaag taaagagtga agagtgaaaa gggaggagta aagagggggg agtgaaaaga
     4921 gggaaagacg tcagtggtga ccgctacgac gtgaaagggc ccccggacgg gatcgcgttc
     4981 gggggccctg ttacgcttta gcgctcacga cctacttctc accttcgtca ctcttcactc
     5041 ttcactcttt acttctcctt ctccttctcc ttcttacttc ttacttctca cttctcactt
     5101 ctcacctttc agcaggaact tgtcccacac catcgcggcc acgatggcgc cgagcaccgg
     5161 cccgatccag taggcgaggt ggccggtcca ctgcatcgtg acgagcgagg ggccgaacgc
     5221 gcgggcggga ttcatgatgg cgccggtgag gggcccgccc gccaggatca tcacgaacac
     5281 cgtgagcccg acgccgaagc cgcccatccg ggtggccttg ggggacacga tcgtggcgta
     5341 caccgcggtc atcaggaaga aggtgaggat ggcctcgatg ccgatggcgc cggcgaagga
     5401 gatcgccgag tggatcgtcg ggacgccgag gtcggtcacg gacgtgacgc cgcccgggta
     5461 ggtcgccttg acggccagcg cgccgagcac gccgccgagg agctgcgcgg cgatgtacca
     5521 gagcgccttc ttgaggtcga tccggcccac ggacaggaag cccagcgtca ccgccgggtt
     5581 cacgtgcccg ccggaatacg ggagggcgat ggtgatcgcc aggccgagcg ccaggccgtg
     5641 cgccagcgcg atgcccagca tgttgaagcc gacgagggga tagttgttcg ccatcatcgc
     5701 cgcgacgccg acgaagacga gcgtgaacgt gccgagcgct tcgacgaaca gcggacgggc
     5761 gagggaacgc atccggtggc ctcggggtgg ggtgatacga tgtgcgggaa cgggcggaag
     5821 tctaggcggg ccgggcgtgg ggcgccagtt gggtggcaga tggcgggcgg ggatcacgcc
     5881 acgtcgggga gatcgcacgc acctcgtcgc tcgtcacccg cccgcactca ccgcccatcc
     5941 gcccgccgga acaggagcgc cgcattgatc ccgccgaagc cgaaggagtt gctgagcagg
     6001 tactccgggt cggcgtcgac cccgtcacca cgcaggtagg ccaggtcgca ggcggtgtcg
     6061 ggggagtcga ggttgaccgt cggcgggagc cagcggcggc ggctcgcgag cgcgcagatg
     6121 gcggcctcga aggcgccgct cgcgccgagc gcgtggccgt aatatccctt ggtgccgctc
     6181 acggcgagcg ccgtggcatg cgggccgaac gcgcgccgga tggccagcgt ctcggtcggg
     6241 tcgttgagcg ccgtcgccga accgtgggcg ttcacgtacc ccaccgacgc cgccggcacg
     6301 tgcgcatccg cgagcgccag cgtgatggcc cgcgcggcct gcgccccgtc gggtcgcggt
     6361 gcggtcatgt ggtgcgcgtc gttggtggtg ccgtagccgc acacttccgc atacaccggc
     6421 gcgcctcggg ccagcgcccg cccgcgctcc tccagcacca gcaccgccga gccctcggcc
     6481 atcacgaacc cgtcccgccc ccggtcgaag ggccggctcg ccgccgccgg atcgtcattg
     6541 cgggtcgaca tcgcgcggat gatcgagaac gcgccgaagg agagcggcgc gagcggcgcc
     6601 tccgcgccac ccgccagcat cacgtcggcc tcgccgcgcc ggatggtgcg gtacgcgtcg
     6661 ccgatggcca tcgccccgga ggcgcagctc atcccgttgg tggcgttggg cccggtcagg
     6721 ccgaactcga tcgcgagatt gcagctggct gcgccggcga acacggtcag cgcgagcccg
     6781 gggtcgacat cgcggacgcc gccccggagg tagcgcgcgt gctgctcctc cgcgtgcgcc
     6841 acgccgccga gcgcggtgcc catcatcgcg ccggtgcggt cggagtcctc gcggcggagg
     6901 tcgagcgccg cgtcggaaag cgccaggcgc gccgccgcca gcgtgaactg ggagcagcgg
     6961 tccatgcgcc ggacccgccg ctcctcgacg aaatcggagg ggtggaagtc gcggacctcg
     7021 cccgccaccc gggtgcggaa gggcgtcgca tcgaaccggg tgaccggtcc gaccgcggac
     7081 cggcgtgcgc ggagccccgc ccacagcggc tcggcgccgg tgccgatcgg cgtgacggcc
     7141 ccgatgccgg taatgaccac ccggcgcggc tcagccatcg gcccgctcca ccacccgggc
     7201 gatcccggcc agcgtccgcg acgcgatccc gtgcacgaac accggaccga tgatccatgc
     7261 ggcggccgca cggccgatga ggggccaccg ggggccggtc cactcgtgca cgatggtgac
     7321 cacggtcccc tcgccttcgc tcgagaagct ccactccacg tccattccgc gcgtgatccc
     7381 gcggatgtgc cggtagcgca ccgccggccg cgaccggtcg atccacatct ccgacgtcca
     7441 ccaggtcggc cagttgagcg acccgaacgg ccgccacgcg gccatctcca ccgtcgcggc
     7501 gccgccctcg ccgcccggcg tcagccggac ccaccgatag tgcggcagcc attc
//
LOCUS       DASUAP010000267        13185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_948510, whole genome shotgun sequence.
ACCESSION   DASUAP010000267 DASUAP010000000
VERSION     DASUAP010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948510"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..419
                     /locus_tag="VFU45_07000"
     CDS             150..419
                     /locus_tag="VFU45_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358847.1"
                     /translation="MDGFVGSLTGLIAVLAIFGVPGVWLLGKSDIGKAIVERIRHGTH
                     GLPDPQLLAELDEMKDQLAEVQDRLEFAERQLAALKPGTDRTSSK"
     gene            492..749
                     /locus_tag="VFU45_07005"
     CDS             492..749
                     /locus_tag="VFU45_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358848.1"
                     /translation="MEDILAIILIFGGGTAIAISFSPIGRAIAERIRGKPAFAEPDPQ
                     VLAELDDLRGQLAELQERMDFSERLLGQARGTRAEEGGAGA"
     gene            746..1114
                     /locus_tag="VFU45_07010"
     CDS             746..1114
                     /locus_tag="VFU45_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358849.1"
                     /translation="MNSKDSQLTYFLVFGALLVVVPVSLALMFASSRMGPASIPIWFA
                     VSGAGWLISKGPIGQAIAARIHGAPDQSELPEQVLAEIDQLRDRVAELEERQDFSERL
                     LARKHEEALARPTRGHDEPE"
     gene            1101..1370
                     /locus_tag="VFU45_07015"
     CDS             1101..1370
                     /locus_tag="VFU45_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358850.1"
                     /translation="MNPSDIFDIIMATIFGLILPLGLTSLIVFARARSRRMELEARGR
                     TDPALLAELDQLRTHMAEVDERLDFAERLLAQRTDRPELRAEGGS"
     gene            1381..1665
                     /locus_tag="VFU45_07020"
     CDS             1381..1665
                     /locus_tag="VFU45_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358851.1"
                     /translation="MSSQGDLVQVIVIIGSLILFGRLVRYIGPALARRVGGEADGGRR
                     ADALQADVDDLKARLADRAHDRDRLLELEERLDFTERMLAKPSDVRRTEH"
     gene            1666..1941
                     /locus_tag="VFU45_07025"
     CDS             1666..1941
                     /locus_tag="VFU45_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358852.1"
                     /translation="MDFETVVKVLVAVAAAVAIPVGAYAAIVATRAIWVKGEPGKFSA
                     AEAAALRERVEELEAQVARLGELEDRLDFTERMLSQARSAGSLREEN"
     gene            1943..2257
                     /locus_tag="VFU45_07030"
     CDS             1943..2257
                     /locus_tag="VFU45_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358853.1"
                     /translation="MLQAIPPVPAPPSPAVFMDGPPVSVVLITVVVVVAGVIFLYPLV
                     RAWARRLEGGVPRDDSDLQLGARVEHLEQRLADAEERLDFAERMLARGPETAALPREK
                     AD"
     gene            2313..2708
                     /locus_tag="VFU45_07035"
     CDS             2313..2708
                     /locus_tag="VFU45_07035"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEU5358854.1"
                     /translation="MITAEDVEGFLDRLDGGTLQSRAAGEGLWLVRTPDGLEVAVNAV
                     APLLVLRINVMRLPKDPKKLAALCRKLLEFNAKDLTHGAYGIEGDDVVLTDTLALERT
                     DFQEFEASFDTMTLALAQHFGALAPYREN"
     gene            2713..3492
                     /locus_tag="VFU45_07040"
     CDS             2713..3492
                     /locus_tag="VFU45_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEU5358855.1"
                     /translation="MGFWDRLSALIRSNVNDLISRAENPEKMLNQLITDMRGQLAKAK
                     QQVAAAIADEKKLQADAEAMKKQAEDWERRAMLAVQENRDDLAKQALVRYNEALQGAQ
                     QLHETWVKHKAETESLKGSLRSLNDKIEEAKRKKNILIARARRAEAQQRIQETMSGMS
                     DKSAFESFERMQEKIENQERKAIAAAELQQEFEGDTLMQQFQSLEYHGSSDQQLLELK
                     QKMGLLGAGGTAGAKQLGKGGEEVVHDARVVDDDEKGATSQ"
     gene            3675..4760
                     /locus_tag="VFU45_07045"
     CDS             3675..4760
                     /locus_tag="VFU45_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEU5358856.1"
                     /translation="MPAPRFLILADGDFTPMTSKTANAVIRYRPDRVTGVVDRQQAGR
                     TVQDVLGFGGATPVLGSVTEGLARGFDVLLIGIAPQGGRLPDAWKGWILEAIEAGKDV
                     WSGLHTFLGDDPVLAEAARRRQVRLWDIRRPPADLPVATGLARDLAPFVILTVGTDCN
                     VGKMTAQLQLVERLNARGVRTRFVPTGQTGIMIEGWGIGVDAVVADFIAGAAERLTLE
                     GAKDADIVLVEGQGSINHPGYSGVTLGLLHGACPDALLLCHQSSRRYIGDYREASWAK
                     IPPLSTYVRWYEEIGGMVHPTRTIGVCLNTYDLDEAAARAACDAAQQDTGLVATDPVR
                     FDPAPLVEAILAAREAYLGRQKSLRRN"
     gene            complement(4736..5539)
                     /locus_tag="VFU45_07050"
     CDS             complement(4736..5539)
                     /locus_tag="VFU45_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5358857.1"
                     /translation="MEPAVNQALLILRFELAEVRRSRAVLGYGLLLLAVTDALLRFGG
                     GGPRAVVSLMNVVLIFVPLVSVVFGTMHVYAAREFTELLLVQPVSRRSLYAGLLGGLA
                     LPLSAAVTLGVGLPFLWHPAEGGPVAGPLGTLIAAGVLLTLIFTGVAFFAANRFEDRA
                     KGLGAGLLAWLTLTVVYDAFILFVVTTWGDYPVETPLLAMSLLNPVDAARIALLLQLD
                     TAALLGYTGAALQRFFGTPWSIAAAGMALCTWAALPAWAGWRQFRRKDF"
     gene            complement(5524..6297)
                     /locus_tag="VFU45_07055"
     CDS             complement(5524..6297)
                     /locus_tag="VFU45_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358858.1"
                     /translation="MTAPALHLLDAAPVAVPGPGVEFADVHKRFGRVDVLRGVTADLA
                     PGRITAILGPNGAGKSTLIKCLLGLDRADSGRIDVDGRTPGDDPAARARIGYAPQSPR
                     FPENSTGRQILAMLGALRGAPTAGDELLFAEFGLERELDKPTRTLSGGTRQKLSVALA
                     LAFRPPLLVLDEPTAGLDPVASGILKDRLLNERRRGHTVVLTSHLLGEIEDLADDIVL
                     LLEGRVRFLGPLRQLLAATGETRLDRAVAHLMTLEWSRP"
     gene            complement(6294..7517)
                     /locus_tag="VFU45_07060"
     CDS             complement(6294..7517)
                     /locus_tag="VFU45_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEU5358859.1"
                     /translation="MIPALLLAVAALQGARPPLVVRPDGPLPTLTAALGAARPGDTVI
                     VRGGTYREPVIHVDVPVTIIGEGSPVFDGSGAHEIIVVRADGVAIRGLVLENVGASYT
                     DDRAALRLEGVHGCRIEGNRVVNGFFGVYASRVDGCVIARNVITGTGKGESANANAIH
                     LYASRNVTVDGNAVSGHRDGIYLEFTRRSVIRNNVSSRNTRYGLHFMFSDSCDYTANV
                     FRDNAAGVAVMYSHTVTVTGNEFSGSRGSASYGLLLKELRDSRVERNRFVRNTVGLYL
                     EGSDRTFVAGNEFTDCGWGVKLMADADDAAFEGNRFAGNSFDVATNSRSTRARFHANN
                     WDRYAGYDLDHDGFGDVPFRPVRLFALAVEQHPPAIILARSFFMDLLDTAERVLPVLT
                     PEAISDDRPLMRWPG"
     gene            complement(7514..7954)
                     /locus_tag="VFU45_07065"
     CDS             complement(7514..7954)
                     /locus_tag="VFU45_07065"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEU5358860.1"
                     /translation="MRSRTRLMAAALALACAAPAVRPIPYGTEPCAHCHMVITDARFA
                     AELVSRKGRVWFFDDPACLAAFYASAEVPPGDVALLVVNDFVTPDSALDARTASYLET
                     ARVQTPMGGGIIALRAGHETDSVRSALGGSVLSWDQLPRHPGAR"
     gene            complement(7954..8547)
                     /locus_tag="VFU45_07070"
     CDS             complement(7954..8547)
                     /locus_tag="VFU45_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358861.1"
                     /translation="MTKLSRAVLAAASLVLGLLYVTPLWRIQLVAPQYPEGLGIRIWV
                     DRIVGAQPQDLNSINDLNHSIGMRAIEPDAIPELRFMPWILAGLILTGLLAALLGRRW
                     AVRTWLGLVIAGAAAGLADFWKWGYDYGHNLNPRAAIKVPGMSYQPPVVGVKQLLNFQ
                     ATSWPDVGAWIALAAVAAALWVLWRERTPRAGRTQAT"
     gene            complement(8600..10582)
                     /gene="nosZ"
                     /locus_tag="VFU45_07075"
     CDS             complement(8600..10582)
                     /gene="nosZ"
                     /locus_tag="VFU45_07075"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HEU5358862.1"
                     /translation="MSRPLVSLAVLAALAAGCAKSARVGDAAADAAARVYVAPGHYDE
                     LYAFLSGGFDGQVSVYGLPSGRMLRHIPVFSQNPENGWGYSEQTKPMLMTSWGFVPWD
                     DAHHPELSQTNGVPDGRWLFINGNNTPRIARIDLTRFETDEILEIPNAAGNHASPFVT
                     PHDEYVVSGTRFSVPIPEKDVPIASYKDNFAGVLTFAKVDSATGRMSIAFQIRMPGFD
                     YDLSHAGKGPSDGWAFFTSYNSEEANTLLEVNASQNDKDYIAAINWKRAAECVAQGKA
                     QEEPARYAHNIMGADGIARTEWGSSVKVLEPTNCDGMVYFLPTPKSPHGVDVDPTGEY
                     IVAGGKLATVIPVHSFSKMMKAIAAKDFAGEVHGIPVLKYESILAGEVQNPGLGPLHT
                     EFDGKGNAYTSMFISSEIVKWSLSTFKVLDRVPTYYSIGHLMIPGGDSRQPYGKYVVA
                     LNKITKDRYLPTGPELTQSAQLYDITGDRMKLLLDFPTIGEPHYAQAIPASLIKDRQL
                     RVYPLAENRDPYVARSEKETKVVRQGRSVHVYMTAIRSHFVPDNIEGVRVGDSVFFHV
                     TNLEQDWDVPHGFAAMGNNNAELLVMPGQTRTLTWVPQRVGVYPFYCTDFCSALHQEM
                     QGYVRVSAPGATVPLAWNGPNGTLKAPPAADHEMTH"
     gene            complement(10629..11099)
                     /locus_tag="VFU45_07080"
     CDS             complement(10629..11099)
                     /locus_tag="VFU45_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5358863.1"
                     /translation="MRLLSQSLLASLVVLTAACGGDKTSSSDSAGAAEGPPALTPFQQ
                     ENGVGPITQVVPTGPIDKAMAERGEKTFQANCTACHKLDERYVGPALRGVTDRRTPTY
                     VMNMILAPDTMYTRHPVARGLLAQFATQMPNLHLSETQARELMEYLREASAEKH"
     gene            complement(11220..11636)
                     /locus_tag="VFU45_07085"
     CDS             complement(11220..11636)
                     /locus_tag="VFU45_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358864.1"
                     /translation="MPPLSRWFVRTALLELTLGFLLGAAMLATKGSHLWGATARLLPL
                     HIELMLPGWLANLAIGVAYWMLPKHAVGAERGAALPVALAWLLLNGGILLVGSGAAVA
                     VGRSAEFCAVVLFTINAWPRIKRFGAGRPGGQPSAG"
     gene            complement(11636..12043)
                     /locus_tag="VFU45_07090"
     CDS             complement(11636..12043)
                     /locus_tag="VFU45_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358865.1"
                     /translation="MSRLSRWLVRTAFLYLVVALALGVLLVWRPALAQWPVLRRAAPT
                     QLHLFVVGWITQLIAGVAYWLFPRTARGAPPEDWRGWWVYGGLNLGLLLRAAAEPWAG
                     SRWSAGVLPLSAALQFGAGLLLAITLWPRARAR"
     gene            12306..13013
                     /locus_tag="VFU45_07095"
     CDS             12306..13013
                     /locus_tag="VFU45_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5358866.1"
                     /translation="MPDLLSPKQLAEYLQLSQRTVYRLLERGDLPAVKVGGQWRFRKA
                     AVDEWLDVNMQRLDVASLHALEADEEVGRPSRSIAELIAASNAQITVPAGPAEAVVRA
                     FVRAVQYPEPVSVDLVCERVLERERLCSTALSGGVALLHTPRSRPRLLNEHDLVAIGR
                     VEAPIAFGAFDGSPTQTLILLLARSERDQLVLLAKLTRLVQESGFLDAFHATRSAADV
                     IALVRETEHRVFASSSR"
BASE COUNT         2090 a         4340 c         4823 g         1932 t
ORIGIN      
        1 atccgaccat tcggtcaccg cccagatcga cggcgaattg caggacatgc gcctccaggt
       61 cgaggacctg cggcaggcgc tcactgagac gcacgaccgg ctcgacttca ccgagcgcct
      121 gctggcgggc agcgagaagc ggacctgaca tggacggatt cgtgggcagc ctgactgggt
      181 tgatcgcggt gcttgccatc ttcggcgtgc ccggcgtgtg gcttctcggc aagtcggata
      241 tcggcaaggc catcgtggag cgcatccggc acggcaccca cggtcttccg gatccccagc
      301 tccttgccga gctggacgaa atgaaggatc agctggccga agtgcaggat cggctggaat
      361 tcgccgagcg ccagctggcg gcgctcaagc ccggcactga ccgtaccagc agcaagtagc
      421 tggtcgctga tagccgatcg ctgatagctg acagctcttc caacgtccaa cgacgggcga
      481 ccaccgaccc catggaagac atcctcgcga tcatcctgat cttcggtggc gggaccgcca
      541 tcgcgatctc gttcagtccc atcgggcggg ccatcgccga gcggatccgc ggcaagccgg
      601 cgttcgcgga gcccgacccg caggtcctgg cggagctcga tgacctgcgc ggacagctgg
      661 ccgagcttca ggagcggatg gatttctccg agcggctcct cggccaggcg cgaggcactc
      721 gcgccgagga aggcggtgca ggagcgtgaa cagcaaggac tcgcagctca cgtacttcct
      781 cgtgttcggg gcgctgctcg tggtggtgcc ggtgtccctc gcgctgatgt tcgcaagctc
      841 caggatgggg cctgcctcga tcccgatctg gttcgcggtg tcgggggcgg gatggctcat
      901 cagcaaagga ccgatcggcc aggcgatcgc ggcgcggatc cacggcgccc cggaccagtc
      961 cgaactcccg gagcaggtcc tggccgagat cgaccagctc cgcgaccggg tggccgagct
     1021 cgaggagcgg caggatttct cggagcggtt gctggcgcgg aagcacgagg aggcgctggc
     1081 caggcctacg cgagggcacg atgaacccga gtgacatctt tgacatcatc atggcgacga
     1141 tattcgggct gatcctgccc ctcgggttga catcgctgat cgtctttgcg agggcccgct
     1201 cccggcggat ggaactggag gcccggggac gcaccgatcc ggcgctgctg gcggaactgg
     1261 atcaattgcg aacgcacatg gccgaggtgg atgaacggct cgacttcgcg gagcggctgc
     1321 tggcgcagcg cactgatcga ccggagttgc gggcagaggg gggcagctga gatgggtccg
     1381 gtgagctcgc agggggacct ggtccaggtc atcgtgatca tcggctcgct gatcctcttc
     1441 ggtcgcctgg tgcgttacat cggcccggcg ctggcgcgga gggtcggtgg tgaggcggac
     1501 ggaggccggc gggccgacgc gctccaggcc gacgtcgacg atctcaaggc ccggctggcc
     1561 gaccgggcac acgatcgcga ccggctgctc gagctcgagg agcgcctcga cttcaccgag
     1621 cggatgctcg ccaagccgtc cgacgtgcgg cgcacggagc actgagtgga cttcgagacg
     1681 gtcgtgaagg tgttggtcgc cgtggcggcg gcggtcgcga tcccggtcgg ggcctacgcg
     1741 gcgatcgtag ccacccgggc catctgggtg aagggcgaac cggggaagtt cagcgccgcg
     1801 gaggccgcgg cgttgcggga gcgggtggag gagctcgagg cccaggtggc gcggctgggc
     1861 gagctcgaag accggctgga cttcaccgag cggatgctgt cccaggcacg atcggccggg
     1921 tccttgcggg aggagaactg aaatgctgca ggccatcccg ccggtcccgg cgcccccgtc
     1981 gcccgcggtg ttcatggacg gcccgccggt gagcgtcgtt ctgatcacgg tcgtcgtggt
     2041 cgtggcgggg gtcatcttcc tgtacccgct ggtccgcgcg tgggcccgcc gcctggaggg
     2101 gggcgtcccc cgcgacgaca gcgacctgca gctcggcgcg cgggtggagc acttggaaca
     2161 gcggctcgcg gatgccgaag agcggctcga tttcgccgag cggatgctgg cccgcggccc
     2221 cgaaacggcc gccctgccgc gggaaaaggc ggactgacgg acgggctcgg gaaggccccg
     2281 gcggggttac attcttggcg ggagggaacg cgatgatcac ggcggaagac gtcgaggggt
     2341 tcctcgaccg gctggatggc ggcaccctgc agtcccgcgc cgcaggcgag gggctctggc
     2401 tggtccgcac cccggacggg ctggaggtcg cggtcaacgc agtggcgccg ctcctggtgc
     2461 tccggatcaa cgtgatgcgg ctccccaagg acccgaagaa gctggccgca ctctgccgca
     2521 agctgctcga gttcaatgcc aaggacctga cgcacggcgc ctacggcatc gagggtgacg
     2581 acgtggtgct caccgacacc ctggcgctcg agcggacgga tttccaggag ttcgaggcga
     2641 gtttcgacac gatgacgctg gccctggccc agcacttcgg cgcgctggcg ccctatcggg
     2701 agaactgacc ccatgggatt ctgggatcga ctgtcggccc tcatccggtc gaacgtgaat
     2761 gacctcatct cgcgggccga gaacccggag aagatgctca accagctgat caccgacatg
     2821 cggggtcagc tggccaaggc caagcagcag gtcgcggccg ccatcgccga cgagaagaag
     2881 ctccaggccg acgccgaggc gatgaagaag caggccgagg actgggagcg ccgggcgatg
     2941 ctggcggtgc aggagaaccg cgacgacctg gccaagcagg cgctcgtgcg gtacaacgag
     3001 gccctgcagg gcgcgcagca gctccacgag acctgggtca agcacaaggc cgagaccgag
     3061 agcctcaagg gctcgctccg gtcgctgaat gacaagatcg aggaggccaa gcgcaagaag
     3121 aacatcctga tcgcgcgggc ccggcgggcc gaggcgcagc agcgcatcca ggagaccatg
     3181 tccgggatgt cggacaagag cgccttcgag agcttcgagc ggatgcagga gaagatcgag
     3241 aaccaggaac ggaaggccat tgcggcggcg gagctgcagc aggagttcga gggcgatacc
     3301 ctgatgcagc agttccagtc gctggagtac cacgggagct cggaccagca gctcctggag
     3361 ctcaagcaga agatgggcct cctgggcgcc ggcggcacgg cgggcgccaa gcagctcggg
     3421 aaggggggcg aggaagtggt gcacgacgcc cgggtggtcg acgacgacga gaagggcgcg
     3481 accagccagt gagcggtcag gagtgagcgg tgagcgggct cccatcggag aaacgcgatg
     3541 ggggcccgct cacttcttcc cggtcatttc cagccgcacc tatattgctg cggtcgagct
     3601 cgacgacccg tgcccgctca ctactgaccg ctcaccgctc actgcgcacc tctcacctct
     3661 cacctcttcc cgccatgcct gctccccgct tcctgatcct ggccgacggc gacttcacgc
     3721 cgatgacatc gaagaccgcc aacgccgtca tccgctatcg ccccgaccgg gtgacgggcg
     3781 tggtggaccg ccagcaggca ggacggaccg tgcaggacgt cctcggcttc ggcggggcga
     3841 ccccggtcct cggctcggtg acggaagggc tggcccgcgg attcgacgtg ctcctcatcg
     3901 gcatcgcccc gcaggggggc cgccttcccg acgcatggaa ggggtggatc ctcgaggcga
     3961 tcgaggccgg aaaggacgtc tggagcggcc tgcacacctt cctgggtgat gaccccgtcc
     4021 tggccgaggc ggcaaggcgg cggcaggtgc gcctctggga catccggcgc ccgcccgccg
     4081 acctgccggt ggccaccggg ctcgcgcgcg acctcgcgcc attcgtcatc ctgacggtgg
     4141 gtaccgactg caacgtcggc aagatgacgg cgcagctgca gctggtggag cgcctcaatg
     4201 cccgcggcgt gcgcacgcgg ttcgtgccca cgggacagac cggcatcatg atcgaagggt
     4261 gggggatcgg tgtcgacgcg gtggtggccg acttcatcgc cggcgcggcg gagcgcctga
     4321 ccctcgaagg ggccaaggac gccgacatcg tcctggtcga gggacagggg agcatcaacc
     4381 acccggggta ctcgggcgtc acgctcggcc tcctgcacgg cgcctgccct gacgcgctcc
     4441 tgctgtgcca ccagtccagc cgccggtaca tcggcgacta tcgcgaggcg tcatgggcga
     4501 agatcccgcc gctctccacc tacgtccggt ggtacgagga gatcggcgga atggtgcacc
     4561 cgacgcggac gatcggcgtc tgcctcaata cctatgacct cgacgaggct gcggcgcgcg
     4621 cggcgtgcga tgcagcgcag caggacaccg gcctcgtggc aacggacccg gtgcgcttcg
     4681 acccggcgcc gctggtggag gcgatcctgg cggcgcggga ggcatacctc gggcgtcaga
     4741 agtccttgcg gcggaactga cgccagccgg cccacgccgg caatgccgcc caggtgcaga
     4801 gcgccatccc cgccgccgcg atgctccacg gcgtgccgaa gaaccgttgc agcgccgcac
     4861 cggtgtagcc caggagagct gcggtgtcga gctgcaggag cagggcgatc cgggcagcgt
     4921 cgacgggatt gaggaggctc atcgcgagca gcggggtctc gaccgggtag tcgccccagg
     4981 tcgtcacgac gaagaggatg aatgcgtcgt agacgacggt gagcgtgagc cacgccagca
     5041 acccggcccc gaggcccttg gcccggtcct cgaatcgatt ggccgcgaag aacgccaccc
     5101 ccgtgaagat gagcgtgagc aggaccccgg ccgcgatgag cgttccgagc ggccccgcca
     5161 cgggcccgcc ctcggcagga tgccagagga acggcagccc caccccgagg gtgacggcgg
     5221 ccgaaagcgg cagggccagc ccgcccagga gcccggcata gaggctccgc cggctcaccg
     5281 gctgcaccag cagcagttcc gtgaattccc gtgccgcgta cacgtgcatg gtgccgaaca
     5341 ccacgctcac cagcggcacg aagatcagca ccacgttcat caggctcacg accgcccggg
     5401 ggcccccgcc cccgaagcgg agcagcgcgt cggtgaccgc gagcagcagg agcccatagc
     5461 cgagcacggc ccggctgcgg cggacctcgg cgagctcgaa gcggaggatc agcagcgcct
     5521 ggttcacggc cggctccatt cgagcgtcat caggtgcgcc acggcgcggt cgagccgggt
     5581 ctcgccggtc gcagcgagga gttgccggag cgggccgagg aaccggacgc gcccctcgag
     5641 caggagcacg atatcgtcgg ccaggtcctc gatctcgccg aggaggtgcg acgtgagcac
     5701 cactgtgtgc ccgcggcgcc gctcgttgag caggcggtcc ttgaggatcc cgctcgcgac
     5761 gggatcgagg cccgctgtcg gttcgtcgag aacgaggagc gggggccgga aggcgagggc
     5821 cagtgccacg ctcaatttct gccgggtgcc gccggagagg gtgcgggtcg gcttgtcgag
     5881 ctcccgttcg aggccgaact cggcgaagag gagctcatcg ccggccgtgg gtgcgccgcg
     5941 gagcgcgccg agcatggcga ggatctggcg cccggtggag ttctccggga agcgagggga
     6001 ctgcggcgcg tatccgatgc gggcccgtgc cgccggatca tcgcccgggg tgcggccgtc
     6061 gacgtcgatg cggccgctgt ccgcgcggtc gagcccgagg aggcacttga tcagcgtgga
     6121 cttgccggcg ccgttggggc ccaggatggc ggtgatgcgc ccgggggcga ggtccgcggt
     6181 gaccccgcgg agcacgtcca cccgcccgaa gcgcttgtgc acgtcggcga actcgacgcc
     6241 cgggccaggg accgcgaccg gggccgcgtc gaggaggtga agggcggggg cggtcatccg
     6301 ggccaccgca tcagcgggcg gtcgtcgctg atcgcctcgg gcgtcaggac gggcagcacc
     6361 cgttcggccg tgtcgaggag gtccatgaag aagctccggg cgaggatgat cgccggcggg
     6421 tgctgttcca cggccagcgc gaagaggcgg accggccgga acggcacgtc gccgaacccg
     6481 tcgtgatcga ggtcgtaccc ggcgtagcgg tcccagttgt tggcgtggaa ccgggcccgg
     6541 gtgcttcggc tgttggtcgc gacgtcgaat gagtttcccg cgaagcgatt cccctcgaag
     6601 gcggcgtcgt ccgcgtcggc catcagcttg accccccagc cgcagtccgt gaactcgttg
     6661 cccgccacga acgtccggtc cgacccctcg agatagagcc ccacggtgtt ccgcacgaac
     6721 cggttccgct ccactcggct gtcgcgcagc tccttgagga gcagcccgta ggacgccgag
     6781 ccccggctcc ccgagaactc gttgcccgtc acggtgacgg tgtgggagta catcaccgcc
     6841 accccggccg cgttgtcgcg gaacacgttg gcggtatagt cgcaggagtc ggagaacatg
     6901 aagtggagcc cataccgggt gttgcgtgag ctcacgttgt tccggatgac cgaccggcgg
     6961 gtgaattcca ggtagatccc gtcgcggtgg ccgctcacgg cgttgccgtc caccgtcacg
     7021 ttccgggacg cgtagagatg gatcgcgttg gcgttggccg actcgccctt gccggtgccg
     7081 gtgatgacgt tgcgcgcgat gacgcagccg tcgacgcggg acgcgtacac gccgaagaac
     7141 ccgttcacca cccggttacc ctcgatgcgg catccatgca cgccctccag ccgaagcgcg
     7201 gcgcggtcgt cggtgtagct cgcgcccacg ttctccagca ccagtccccg gatggccacg
     7261 ccatccgccc ggaccacgat gatctcgtgc gcgccgctcc cgtcgaacac cggcgatccc
     7321 tcgccgatga tcgtgaccgg cacgtcgacg tggatgaccg gttcacggta ggtgccgccg
     7381 cgcacgatga ccgtgtcgcc cggtcgggcg gccccgagcg cggcggtgag cgtggggagg
     7441 ggcccgtcgg gccggacgac gagcggcggg cgggctccct ggagcgccgc aaccgccagc
     7501 aggagcgccg ggatcatcgg gccccggggt gccgcgggag ctggtcccag ctgaggaccg
     7561 agccgccgag cgccgagcgg acgctgtcgg tctcgtggcc ggcgcggagc gcgatgatgc
     7621 ctccccccat cggggtctgc acccgggcgg tctcaaggta ggaggcggtc cgcgcgtcga
     7681 gcgccgagtc cggcgtcacg aagtcgttca ccacgagcag cgccacgtcg cctggcggca
     7741 cctcggccga ggcatagaag gccgcgaggc aggcggggtc gtcgaagaac cacacgcgcc
     7801 ccttgcggga gaccagttcc gccgcgaacc gtgcgtcggt gatcaccatg tggcagtggg
     7861 cgcagggctc ggtgccgtac gggatcggcc gcacggccgg cgcggcgcag gcgagcgcga
     7921 gcgccgccgc catgagccgg gtgcgggatc gcatcaggtc gcctgcgtgc ggcccgcccg
     7981 cggcgtccgt tcgcgccaga ggacccagag ggccgctgcg accgccgcga gcgcgatcca
     8041 ggcccccacg tcgggccacg aggtcgcctg gaagttgagc agctgcttga cccccaccac
     8101 gggcggctgg tacgacatcc cgggcacctt gatcgccgcg cgcgggttga ggttgtggcc
     8161 gtagtcgtag ccccacttcc agaagtccgc gagccccgcc gctgcgccgg cgatgacgag
     8221 cccgagccag gtgcggaccg cccagcggcg gccgaggagc gccgcgagga ggcccgtcag
     8281 gatgagtccg gcgaggatcc acggcatgaa gcgcagctcg gggatggcgt cgggctcgat
     8341 ggcgcgcatg ccgatagagt gattgaggtc gttgatgctg ttgaggtcct gcggctgggc
     8401 gccgacgatc cggtccaccc agatgcggat gccgagccct tccgggtact gcggcgccac
     8461 cagctggatg cgccagagcg gcgtgacgta caggaggccc agcaccagcg acgccgcagc
     8521 gaggactgcg cgagagagtt tggtcatcgg ggaacgggct ccccggccgg cgcgcggggc
     8581 gccgaccggg ggtgccggct cagtgggtca tctcgtggtc cgcggccggc ggcgccttga
     8641 gcgtgccgtt ggggccgttc caggccagcg ggaccgtggc cccgggggcc gagacccgga
     8701 cgtatccctg catctcctgg tgcagcgcgg agcagaagtc ggtgcagtag aacgggtaca
     8761 cgccgacgcg ctgcggcacc caggtgagcg tgcgcgtctg acccggcatg acgaggagct
     8821 cggcgttgtt gttgcccatc gccgcgaagc cgtgcggcac gtcccagtcc tgttcaaggt
     8881 tggtgacgtg gaagaacacc gagtccccca cccggacccc ctcgatattg tcgggcacga
     8941 agtggctccg gatggcggtc atgtacacgt gcaccgaccg cccctgccgg acgaccttcg
     9001 tctccttctc gctccgcgcg acgtacgggt cgcgattctc ggcgagcgga tacacccgca
     9061 gctgccggtc cttgatgagg ctcgccggga tggcctgggc gtagtggggc tcgccgatcg
     9121 tcgggaagtc gagcaggagc ttcatccggt cgccggtgat gtcgtagagc tgggccgact
     9181 gcgtgagctc cgggccggtc gggaggtagc ggtccttggt gatcttgttg agtgccacga
     9241 cgtacttccc atagggctgc cgcgagtcgc cgccggggat catcaggtgg ccgatggagt
     9301 agtaggtcgg cacccggtcg agcaccttga aggtcgacag gctccacttc acgatctcgg
     9361 agctgatgaa catcgacgtg taggcgttgc ccttgccgtc gaactcggtg tggagcgggc
     9421 cgaggcccgg gttctggacc tcaccggcca ggatcgactc atacttgagg accgggatcc
     9481 cgtggacctc gccggcgaag tcctttgcgg cgatggcctt catcatcttc gagaacgagt
     9541 ggaccgggat cacggtggcg agcttcccgc cggccacgat gtactcaccc gtcgggtcga
     9601 cgtccacgcc gtgcggcgac ttgggggtcg ggaggaagta gaccatcccg tcgcagttcg
     9661 tgggctccag caccttcacg ctcgagcccc actcggtgcg ggcgatgccg tcggcgccca
     9721 tgatgttgtg ggcgtaacgc gccggctcct cctgcgcctt cccctgcgcc acgcattccg
     9781 ccgcgcgctt ccagttgatg gccgcgatgt agtccttgtc gttctgcgag gcgttgacct
     9841 cgaggagggt gttggcctcc tcgctgttgt aggaggtgaa gaacgcccag ccgtcggagg
     9901 ggcccttgcc cgcgtgcgac aggtcgtagt cgaagccggg catccggatc tggaaggcga
     9961 tgctcatgcg gccggtggca ctgtccacct tcgcgaacgt gaggacgccg gcgaagttgt
    10021 ccttgtagct cgcgatcggt acgtccttct cggggatcgg cacgctgaag cgggtccccg
    10081 acaccacgta ctcgtcgtgc ggcgtcacga agggcgacgc gtggttgccg gcggcgttcg
    10141 ggatctcgag gatctcgtcg gtctcgaacc gggtcaggtc gatccgcgcg atgcgcgggg
    10201 tgttgttgcc gttgatgaag agccagcgcc cgtccgggac cccgttggtc tgcgaaagct
    10261 ccgggtggtg ggcgtcgtcc caggggacga agccccagct ggtcatcagc atgggcttgg
    10321 tctgctcgct gtacccccag ccgttctccg ggttctggga gaagacgggg atgtggcgca
    10381 gcatccggcc ggaggggagc ccatagacgc tcacctggcc atcgaagccg cccgacagga
    10441 aggcgtacag ctcgtcatag tgcccggggg ccacgtagac gcgcgccgcc gcatcagcgg
    10501 cggcatcgcc cacacgcgcg gatttggcgc agccggccgc gagcgcggcg aggacggcga
    10561 gggacacgag gggtcgggac atctggtgcc tccggaacac gttggacggt atcggttcga
    10621 tggacggttc agtgcttctc ggcggacgct tcccgcaggt actccatcag ctcgcgggcc
    10681 tgggtctcgc tgaggtgcag attgggcatc tgggtcgcaa attgggccag caggccccgg
    10741 gccaccgggt gcctggtgta catcgtgtct ggcgcgagga tcatgttcat cacgtaggtc
    10801 ggtgtgcgcc ggtccgtcac gccgcgcagc gccggcccga cgtaccgctc gtccagcttg
    10861 tggcatgcgg tgcagttggc ctggaacgtc ttctcacccc gctcggccat cgccttgtcg
    10921 atcgggccgg tcggaacgac ctgggtgatc ggcccgacgc cgttctcctg ctggaagggc
    10981 gtcagcgccg gcgggccttc agcggcgccg gccgaatccg aggaggaagt cttgtcgccg
    11041 ccgcaggcgg ccgtcagtac gacgagggaa gcgaggaggg actgggacag caggcgcatc
    11101 cgtcgatctc cattcaggat gggggaattc gaacagaata agtctgcttt ccggggggtc
    11161 aggatcggat gaaggcggga tgaatcgttc tttagagggg aaggacttgg aggagcgcgt
    11221 caaccggccg agggctggcc tccggggcgt cccgcgccga atcgcttgat ccggggccag
    11281 gcgttgatcg tgaagagcac caccgcgcag aattccgcag agcgtcccac cgcgacggcg
    11341 gctccgctgc cgacgagcag gatccctcca ttcaacagga gccacgcgag ggcgaccggc
    11401 agggctgccc cgcgctccgc gccgacggcg tgcttgggga gcatccagta cgccacgccg
    11461 atggccaggt tggcgagcca gcccgggagc atgagctcga tgtggagcgg cagcaggcgt
    11521 gcggtggcgc cccagaggtg cgaccccttg gtcgccagca tcgccgcgcc gaggaggaag
    11581 ccgagggtga gttcgaggag cgcggtccgg acgaaccagc ggctgagcgg cggcatcagc
    11641 gggcgcgggc ccggggccag agcgtgatgg cgagtagcag gccggcgccg aactgcaggg
    11701 ccgccgagag cgggagcacg cccgcggacc agcgggaccc ggcccatggc tcggcagcgg
    11761 cgcgcaggag gaggcccagg ttgagcccgc catagaccca ccacccgcgc cagtcctccg
    11821 gcggcgcccc tcgcgcggtg cgggggaaga gccagtaggc caccccggcg atcagctggg
    11881 tgatccatcc cacgacgaac aggtgcagct gggtgggcgc ggcgcggcgg agcaccggcc
    11941 actgcgccag ggccggccgc cacacgagca ggacgccgag ggcgagcgcc accaccaggt
    12001 agaggaacgc ggtgcggacc agccagcggg agagacggga catcggtcct cggtcagcga
    12061 gggatatcgg cagtgagccg gccactgact catgaagcaa cctaacgtcg acgccaggtg
    12121 ctgcctcggt ctcgggtcag ggccgcgaca cgcggtccgg tccggtgggc atggggcgcg
    12181 acccggcttg cagggggggc atggtcaaag gatcggcttg gtcatatgtc agaatatgac
    12241 gatacttgac attgctgcct aaagatggcc atgatttccc cgtcctccgg ccaggaaaac
    12301 ggtcattgcc cgaccttctc agtcccaagc agctcgccga gtatctccag ctgtctcagc
    12361 gcaccgtgta ccgattgctg gagcggggcg atctgccggc ggtgaaggtc ggcgggcaat
    12421 ggcgcttccg gaaggccgcc gtcgacgagt ggctggacgt caacatgcag cggctggacg
    12481 tggcatcgtt gcacgcgctc gaggccgatg aagaggtggg gcgaccgtca cgcagcatcg
    12541 ccgagctgat cgctgcgagc aacgcgcaga tcaccgtgcc ggcgggcccg gccgaggccg
    12601 tggtgcgggc gttcgtgcgc gccgtgcagt acccggagcc ggtctcggtg gacctggtct
    12661 gtgagcgggt gctggagcgc gaacggctct gcagcacggc gctctcgggc ggggtcgcgc
    12721 tgctgcacac gccgcgcagc cgtccgcggc tgctcaacga gcacgatctg gtggccatcg
    12781 ggcgggtgga ggcgcccatc gccttcggcg cgttcgacgg gagccccacg cagacgctca
    12841 tcctcctgct cgcgcgcagc gagcgtgacc agctggtgct gctggccaag ctcacgcgcc
    12901 tcgtccagga atccgggttc ctggacgcgt tccacgccac ccggtccgcg gccgacgtga
    12961 tcgccctcgt gcgcgagacg gagcaccgcg tgttcgcttc ctcctcccgc tgatcgcgca
    13021 cccgaccgcc aaccatgcaa ctcaccctcc gccaggtggc ctcctacctc gatgtccccg
    13081 atgccacggt ccgccgctgg atcgccgatc ggggcctccc cgcccaccgg gtgaacgagc
    13141 agttctactg caacaccatc gaactgtggg aatgggcggt ggaga
//
LOCUS       DASUAP010000268         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1057507, whole genome shotgun sequence.
ACCESSION   DASUAP010000268 DASUAP010000000
VERSION     DASUAP010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057507"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFU45_07100"
     CDS             complement(<1..603)
                     /locus_tag="VFU45_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358867.1"
                     /translation="MRHLLACTVLSALTALPAMAQDTTTYTHADTLKGSLTSPARTWW
                     DVTFYDLHVKVNPADSSISGRNGITYSVVGAPHEMQIDLQAPLGVDSMVQDDRKLRFR
                     QDGNAWFATVNGKQKTGESHTVTVYYHGRPTIAKRPPWDGGFTWGHDSLGKPWIVTTD
                     QGIGASIWWPNKDIQADEPDSQRIAITMPDPLVDVSNGRLR"
     gene            complement(774..1076)
                     /locus_tag="VFU45_07105"
     CDS             complement(774..1076)
                     /locus_tag="VFU45_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU5358868.1"
                     /translation="MVRRHLSAVYILTSRRRSVLYVGVTSDLQRRMFEHRSGMVAGFA
                     RRYSVNRLVWYEWHADIRHAIAREKQIKAGSRADKEALITALNPEWRDLAAEFGSG"
     gene            complement(1234..2268)
                     /locus_tag="VFU45_07110"
     CDS             complement(1234..2268)
                     /locus_tag="VFU45_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU5358869.1"
                     /translation="MRATVMYRAGDVRVENVPDARLIEPTDALVTVRRACICGSDLWP
                     YNLMAPVEGGRRMGHEAIGVIEETGAEVRTLKAGDFVIMPFAFSDGTCDFCHEGLQTS
                     CTHGGFFGTDETAGAQAEAVRVPQADGTLYRIPLDADYALMPSLLTLSDVMGTGHHAA
                     VTARVGPGKVAAVVGDGAVGLCGVIAAKRLGAERIIIMGRHPERIALARRFGATDVVS
                     ERGDEAVARVRDLTGGFGAHSVLECVGNEQAMLTAVRIARPGGAVGRVGVPHYDAVPA
                     TQSVFFENVTVSGGPAPVRAYLDELLPDVLEGRIEPGLVFDRTIGLEEVPDGYRAMNA
                     REAIKVMVRP"
     gene            2343..>2567
                     /locus_tag="VFU45_07115"
     CDS             2343..>2567
                     /locus_tag="VFU45_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358870.1"
                     /translation="MSPSESAVLDIGRVRLVRGEGSWRVSAMIEGDEVYFESAVPLRP
                     SVEAFLSAFLIPAMRTGRSVRTEDAVCGVWA"
BASE COUNT          385 a          859 c          911 g          412 t
ORIGIN      
        1 ccggagccgg ccgttggaga cgtccaccag cgggtcgggc atggtgatgg cgatgcgctg
       61 gctgtccggc tcgtcggcct ggatgtcctt gttgggccac cagatgctgg cgccgatgcc
      121 ctggtcggtg gtgacgatcc agggcttgcc gagactgtca tggccccagg tgaagccgcc
      181 gtcccagggg ggccgcttcg cgatggtggg gcggccgtgg tagtagacgg tgacggtgtg
      241 ggactcgccg gtcttctgct ttccgttcac cgtggcgaac caggcgttgc cgtcctggcg
      301 gaagcggagc ttgcggtcgt cctgcaccat gctgtccacg ccgagggggg cctggaggtc
      361 gatctgcatc tcgtgcggcg cgcccaccac ggagtaggtg atcccgttcc ggccgctgat
      421 ggagctgtcg gcggggttca ccttcacgtg gaggtcgtag aaggtcacgt cccaccaggt
      481 gcgtgcgggg ctcgtgaggg agcccttgag cgtgtcggcg tgggtgtagg tggtggtgtc
      541 ctgggccatg gcgggcaagg cggtcagggc ggacaagacg gtgcaggcga gcaggtggcg
      601 catgggagtc ggggctggag ttggggatgg agggaagcta agggctggag gcggaaagtg
      661 aaggcacggg accaggtacg tcattgcgag ggccgcgggc tcgcgacgac gtgcgaagct
      721 gacgcgtcat tgcgaggaga gcccggaggg ctcgacgaag caatcgggtc gttctatccc
      781 gatccgaact ccgcagccag atcccgccac tccggattca gcgcagtgat gagcgcttcc
      841 ttgtcagccc gactgccggc cttgatctgt ttctcgcgcg cgatggcatg ccggatgtcc
      901 gcatgccatt cataccagac caggcgattt accgagtatc gccttgcgaa tccggcgacc
      961 atgcctgaac gatgctcaaa catgcgccgc tggagatcgc tcgtcacccc gacatagagg
     1021 actgaacgcc gacgcgaggt caggatgtag acggcggaca ggtggcggcg gaccatgctc
     1081 caacatgggc acggcggatc aacgggagat caatgtgcgg ggccatccga tcgcgtcgcc
     1141 cgctggcgcg ggctcgcgat gacgtgtcag ggccacccga ttgcttcgtc gggagcttcg
     1201 ctccctcctc gcaatgacgt gcctaggtgt gccctacggc cgcaccatca ccttgatcgc
     1261 ctcccgcgcg ttcatggcgc ggtagccgtc cggcacctcc tccaggccga tggtccggtc
     1321 gaacaccagc cccggctcga tccgtccctc gagcacgtcg gggagcagct cgtcgagata
     1381 cgcccgcacc ggcgccgggc cgccgctgac ggtgacattc tcgaagaaga ccgactgcgt
     1441 cgcggggacc gcgtcgtagt gcggcacgcc gacccggccg accgccccgc cgggccgggc
     1501 gatccgcacc gccgtgagca tcgcctgctc gttgccgacg cattccagca ccgagtgcgc
     1561 accgaagccg ccggtgaggt cacgcacccg cgcgaccgcc tcgtcacccc gctcgctcac
     1621 cacgtcggtc gcgccgaacc gccgggccag cgcgatccgc tccggatgcc gccccatgat
     1681 gatgatccgc tccgcgccca gccgcttcgc ggcgatcacg ccgcagagac ccaccgcacc
     1741 gtcccccacc accgccgcca ccttcccggg ccccacccgc gcggtgaccg cggcatggtg
     1801 ccccgtgccc atcacgtccg acagcgtcag cagcgacggc atgagcgcgt agtcggcgtc
     1861 gagcgggatc cggtagagcg tgccatccgc ctgcggcacc cgcaccgctt ccgcctgggc
     1921 accggcggtc tcgtcggtcc cgaagaaccc gccgtgggtg cacgaggtct ggagcccctc
     1981 gtggcagaag tcgcaggtgc cgtcggagaa ggcgaagggc atgatcacga agtcgcccgc
     2041 cttgagcgta cggacctcgg cgccggtctc ctcgatcacg ccgatcgcct cgtggcccat
     2101 gcggcgcccg ccctcgaccg gcgccatcag gttgtagggc cacaggtcgc tgccgcagat
     2161 gcaggctcgg cgcacggtca ccagcgcgtc ggtcggttcg atcaggcggg cgtcgggcac
     2221 gttctccacg cgcacgtcgc cggcacggta catcacggtg gcacgcatca ggatctccgg
     2281 aaaggtgcga cgcgtccgcg gcgaggccac gcgagcgtca tgcgtccaac tataatacgg
     2341 ccatgagtcc atcagagtct gccgtgctcg atatcggccg tgtccggctg gtcaggggcg
     2401 aggggtcgtg gcgagtgtcg gcgatgatcg agggcgacga ggtgtacttc gagtcggcgg
     2461 tgccgctccg gccgagcgtc gaggcgttcc tctcggcatt cctgatcccg gcgatgcgga
     2521 ccggacggtc ggtgcgcacg gaggatgcgg tgtgcggcgt ctgggcc
//
LOCUS       DASUAP010000269         7908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_307656, whole genome shotgun sequence.
ACCESSION   DASUAP010000269 DASUAP010000000
VERSION     DASUAP010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307656"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1280
                     /locus_tag="VFU45_07120"
     CDS             111..1280
                     /locus_tag="VFU45_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU5358871.1"
                     /translation="MSPVVTSALPLSLLSEEEELFRSTVAEFAETEVRPWVREMDEAA
                     HFRQDIIPKFFELGLMGIEVPEQWGGAAGSIFLATLAIEELAKVDASAAIYVDVHNTL
                     VNNAVLRWANEEQKARYFPRLTSGLLGAFALSEAGSGSDAFALAARAEPKGDDWILNG
                     HKLWITNGAEAGFFLVFANVDPSKGYKGITAFLVERDFPGFKVGKKEDKLGIRASSTC
                     EILFENCRVPAANVMGPVGQGYKIAIETLNEGRIGIGAQMIGVASGALAAATSYIKER
                     KQFGKAVADFQGVQFQVGQMATELEAARLLVYNAARLKDAGQPFTQQAAMAKLFSSQV
                     AGRITSLCVELLGGYGYTKDYPVEKFYRDAKIGTIYEGTSNMQLQTIAKVLLSAR"
     gene            1277..2797
                     /gene="dacB"
                     /locus_tag="VFU45_07125"
     CDS             1277..2797
                     /gene="dacB"
                     /locus_tag="VFU45_07125"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEU5358872.1"
                     /translation="MSTSRQRGAAAALAALLLASAPLRAQSVSPHLTRLLDGWYKRAQ
                     RTAHGRWGVAIADELGNVLWSRQPDQPLIPASTAKIFTTGFARSRLGGEARIATRVLG
                     TGFLDSASGTWNGTWTLELNGDPSLDDPSHAGPRLYDLAEQLHAAGIRRIEGPMLVSS
                     EAGPATATFPAGWPARHRGAVFAPLIGSLTVHENIITAWVAPTRRSGHRPLLLDDAPA
                     GVRSMIVNEAITRTGRRSRLHLSARSNGWVLTGWIGTRAGMRQVKAVAYDPERVLELA
                     WRQALRQAGVEWDEDPHTVLAPSDGRIRTLAEVFSPTLDTLAADIDRRSLNLGAELLL
                     RWAAGPDAPADSLVEHVARVTGIGAEQFRLVDGSGLSDLDRVTPRLQVTYLARIPTFP
                     ATRAFPLLLPANGTGTLTRLRLGLPGAGVLRAKTGTLGNVAAIAGYLGRPDGVFIVSM
                     IYNGGRTAAARQAQTRLFRLLGAKGTVQPEDFDPPPDASPDSSAIPPDTGSVLPAH"
     gene            complement(2802..4004)
                     /locus_tag="VFU45_07130"
     CDS             complement(2802..4004)
                     /locus_tag="VFU45_07130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5358873.1"
                     /translation="MLNAFYGLIGLEGIVVLATGVVVAFAGRLPGVEALAAILPPVVL
                     VVGGFLAWRFQRGRPLLGLLATAFALTAFQLAGVRSAALLPAGTLVLGWVAILAAVVT
                     AAARERAGATLMSPAAAIVAGAWLVAVPVIVGFEAELSPLLEPRWVPARWVAAAGVPE
                     AVLIPGVVIVATLLGWARWRNDVTARGAAWGLVAGVIGIGSWQAPGAGLYAAAAGAAF
                     LVSAVEEIHAVAFRDPLTGLPARRALEAALARLSAPYVVAMVDVDHFKRFNDTYGHTV
                     GDQVLTMVATRLAQVAGARAFRYGGEEFTLLLPGRSLTDAAPVLEGARAAVEATTFTL
                     RAPDRPRRRPRKGKAKKRGGSEVVAVTVSIGAAEPTAGDRATAVLEAADQALYRAKEG
                     GRNRIAAG"
     gene            4100..4723
                     /locus_tag="VFU45_07135"
     CDS             4100..4723
                     /locus_tag="VFU45_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358874.1"
                     /translation="MKIRIVVAALLIATPLAAQAGADSMTNAGVVRAADAVDSVFLAR
                     TRVSDTVAGGDWAAYLMARLGVKPIPPDLRLKVATDSAAIHIFGHIADLPAEARMQLG
                     PLLSLLDTSTTIDAWASLAQAGPAAVRFRLDSAAVGGMGIPEVALAPVLANVGVRFPV
                     LTRTGRDLFVETPPYGRVELALNGVLLVAPPPPPSAPHTRPRRTRAR"
     gene            4720..4926
                     /locus_tag="VFU45_07140"
     CDS             4720..4926
                     /locus_tag="VFU45_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358875.1"
                     /translation="MSDPASLRRIYRLWRTSAILCGLGAVEFALLAWQRGVAWIAAAA
                     VPLVAVSIFATRRAARARRSWGIR"
     gene            4956..5312
                     /locus_tag="VFU45_07145"
     CDS             4956..5312
                     /locus_tag="VFU45_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEU5358876.1"
                     /translation="MSPILLRTAGLLVLSNVFMTFAWYAHLRNLGDRPVFVAILASWA
                     IAFFEYCFQVPANRIGFTAFSLAQLKILQEVITLGVFVPFALLYMKQPIKLDFLWAGL
                     CMVGAVYFMFRGHVPA"
     gene            5373..5627
                     /locus_tag="VFU45_07150"
     CDS             5373..5627
                     /locus_tag="VFU45_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358877.1"
                     /translation="MTAFQARLKPQVAQKYSGLNSVTWYDVEPLWAGVTERNMNLLGQ
                     RMTRLRTGKDHTTVLAEDLEFREHPARATERTTDRATAQE"
     gene            5732..6241
                     /locus_tag="VFU45_07155"
     CDS             5732..6241
                     /locus_tag="VFU45_07155"
                     /inference="COORDINATES: protein motif:HMM:NF025658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEU5358878.1"
                     /translation="MPRKKTPATTATKAANNPLTDLLKVDNAERFWAGINDLVFPRYE
                     RLGYDGLSQGEQVAHCIDWLVREVEAGGLRQFLSEISGDHTAETLTALRAIGADHTAE
                     MMELATTVFPEEVPIDQDERLAIMEDFTGRERRMLKDFDAGFFDNSEDLLKLLKAYMA
                     KHVAEFHPE"
     gene            complement(6387..7874)
                     /gene="ahcY"
                     /locus_tag="VFU45_07160"
     CDS             complement(6387..7874)
                     /gene="ahcY"
                     /locus_tag="VFU45_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU5358879.1"
                     /translation="MSTTVTTEPRTTSESTDRPPFKVRDLSLAEWGRNEIRLAEQEMP
                     GLMALRSEYAGKRPLAGARIMGSLNMTTQTAVLIETLAALGADVRWVSCNIFSTQDQA
                     AAAVVVGKDGTESDPRGVPVFAWKGETLLEYWWCTEQALMWPDGGGPNLLLDDGGDAT
                     LLVHKGAEYERSGKIPAFDAANDPEEWGVILDLLRAEAKAHPGRWGTVAAGIRGVSEE
                     TTTGVHRLYEMMRAGTLLFPAINVNDSVTKSKFDNIYGCRHSVIDGLNRATDVMIAGK
                     LAVVCGYGEVGKGCAQALKGQGARVVITEIDPICALQASMEGYQVLTLEDVVSTADIF
                     ITATGNRDIITAQHMARMKDKAIIGNIGHFDNEIDMAGLKDIKGVRRVNIKPQYDEWA
                     FPDGHSVLILSEGRLFNLGNATGHPSFVMSASFTNQVMAQIELHQNAGKYETKVYTLP
                     KHLDEKVARLHLDKLGVRLTTLTPAQAKYIGVKPEGPYKAEHYRY"
BASE COUNT         1198 a         2957 c         2545 g         1208 t
ORIGIN      
        1 gctttcgccc acgttcctac gccttcccca gcattccccc gctttcttct ctcccaccct
       61 atcccccgct attttagggc gtccgttccg gcaccacctt cggaggtccg atgtccccgg
      121 tcgtcacgtc cgccctgccg ctcagcctgc tcagcgaaga agaagagctc ttccgctcca
      181 ccgtcgccga gttcgccgag accgaggtcc gcccctgggt gcgcgagatg gacgaagcgg
      241 cgcacttccg ccaggacatc atccccaagt tcttcgagct cggcctgatg ggtatcgagg
      301 tgccggagca gtggggcggc gccgcgggct cgatcttcct cgccaccctc gccatcgagg
      361 agctcgccaa ggtcgacgcc tcggccgcca tctacgtcga cgtccacaac accctcgtca
      421 acaatgcggt gctccgctgg gccaacgagg agcagaaggc ccgctatttc ccccggctca
      481 cgagcgggct cctcggcgcc ttcgcgctct ccgaggccgg gagcgggagc gatgcctttg
      541 ccctcgccgc ccgcgccgag ccgaagggcg atgactggat cctcaacggc cacaagctct
      601 ggatcaccaa cggtgccgag gccggattct tcctcgtctt cgccaacgtc gacccgagca
      661 agggctacaa ggggatcacg gccttcctcg tcgagcgcga cttccccggc ttcaaggtcg
      721 gcaagaagga ggacaagctc ggcatccgcg cctccagcac ctgcgagatc ctcttcgaga
      781 actgccgggt ccccgcggcg aacgtgatgg gccccgtggg ccagggctac aagatcgcca
      841 tcgagacgct caacgaagga cgcatcggga tcggcgccca gatgatcggc gtggccagcg
      901 gggccctcgc ggccgccacg tcgtacatca aggagcggaa gcagttcggc aaggcggtcg
      961 ccgacttcca gggggtccag ttccaggtgg gccagatggc caccgagctc gaggcggcgc
     1021 ggctcctggt gtacaacgcg gcccggctca aggacgccgg ccagcccttc acccagcagg
     1081 ccgcgatggc caagctcttc agttcccagg tggcgggtcg gatcacctcg ctctgcgtcg
     1141 aactcctggg cggctacggc tacaccaagg actacccggt cgagaagttc taccgcgacg
     1201 ccaagatcgg cacgatctac gaagggacga gcaacatgca gctgcaaacg atcgccaagg
     1261 tgctcctctc cgcccggtga gcacctcccg ccaacggggg gcggcggccg cactcgccgc
     1321 cctcctcctg gcctcggcgc ccctccgcgc gcagtcggtc tccccccatc tcacccggct
     1381 gctcgacggc tggtacaaac gcgcccagcg caccgcccac gggcgctggg gggtcgccat
     1441 cgccgacgag ctcggcaacg tcctctggtc ccgccagccc gaccagccgc tcatccccgc
     1501 ttccaccgcc aagatcttca ccaccggctt tgcccgcagc cgtctgggcg gcgaggcccg
     1561 cattgccacc cgtgtcctcg gcaccgggtt cctcgacagc gccagcggca cctggaacgg
     1621 cacctggacc ctcgagctca acggcgaccc gtcgctcgac gacccgtccc acgccggtcc
     1681 gcggctctat gacctcgccg agcagctgca cgccgcgggg atccgccgca tcgaggggcc
     1741 gatgctcgtc tcctccgaag cgggacccgc cacggcgacc ttccccgccg gctggcccgc
     1801 ccggcatcgc ggcgccgtct ttgccccgct catcggctcc ctcacggtcc acgagaacat
     1861 catcaccgcc tgggtggcgc ccacccgccg atccggacat cggccgctgc tgctggacga
     1921 cgcgccggcc ggcgtccgct cgatgatcgt gaacgaggcc atcacacgca ccggcagacg
     1981 gagtcgcctc cacctctcgg cacgcagcaa cgggtgggtg ctgaccggct ggatcggcac
     2041 ccgcgcgggt atgcgacagg tgaaggcagt ggcgtacgac cctgagcggg tgctcgaact
     2101 ggcgtggcgt caggcgctcc gtcaggccgg ggtcgaatgg gacgaagatc cccacaccgt
     2161 gctggcgccg agcgacggca ggatcaggac gctggcggag gtgttctcac cgaccctcga
     2221 cacactcgcc gcggacatcg accgccggag cctcaacctg ggcgccgaac tcctgctgcg
     2281 ctgggcggcg gggcccgatg cacccgccga ttcgctcgtg gagcacgtgg cgcgggtcac
     2341 cgggatcggc gcggagcagt tccggttggt ggacgggagc ggcctctccg acctggaccg
     2401 ggtcactccc cgcctccagg tgacctatct ggcccgcatc ccgacgttcc ccgccacccg
     2461 cgccttcccg ctgctcctgc cggccaacgg caccggaaca ctgaccaggc ttcgtctcgg
     2521 gctccccggc gcgggagtgc tccgcgccaa gaccgggacc ctcggcaacg tcgcggcgat
     2581 cgccggttac ctcggccggc ccgacggcgt gttcatcgtc tcgatgatct acaacggcgg
     2641 ccgcactgcc gccgcccggc aggcacagac ccgcctcttc cgcctccttg gcgccaaggg
     2701 cacggtgcag cccgaggatt tcgatccgcc gcccgacgca tcacccgact cgagcgccat
     2761 ccctcccgac accggcagcg tcctcccggc gcactgagcc gtcacccggc ggcgatccgg
     2821 ttccgaccgc cctccttggc ccggtacaac gcctgatcgg ccgcctcgag caccgccgtc
     2881 gcgcggtctc cggcggtggg ctcggccgcg ccgatgctca ccgtcaccgc gaccacctcc
     2941 gaaccgcccc gcttcttggc tttccccttc cgcggcctgc gccggggccg gtcgggggcg
     3001 cgaagcgtga acgtcgttgc ctcgaccgcc gcccgcgccc cttcgagcac cggcgccgcg
     3061 tcggtgagcg accgccccgg gagcaggagg gtgaactcct ccccgccgta gcggaacgcc
     3121 cgggcacccg ccacttgcgc cagacgggtt gccaccatcg tcagcacctg gtcgcccacg
     3181 gtgtgaccat aggtgtcgtt gaagcgcttg aaatggtcca cgtccaccat cgccaccacg
     3241 tacggcgcgg aaagcctggc cagcgccgcc tccagtgcgc ggcgggccgg gagcccggtg
     3301 agcggatcgc ggaaggcgac agcgtggatc tcctccaccg ccgacaccaa aaaggcggcg
     3361 ccggccgcag ccgcatagag cccggcgccg ggcgcctgcc acgaaccgat cccgatcacg
     3421 ccggccacca agccccaggc cgcgccccgc gccgtcacgt cgttccgcca ccgggcccat
     3481 cccaggaggg tggccacgat caccacgccg gggatgagga ccgcctccgg gacgccggcc
     3541 gccgccaccc agcgggcggg gacccaccgg ggctccagca ggggagacaa ctctgcctcg
     3601 aacccgacga tcaccggcac cgcgacgagc cacgcgccgg ccacgatcgc ggcggccgga
     3661 ctcatgagcg tcgcgccggc ccgttcgcgc gccgcggcgg tgacgaccgc ggcaaggatc
     3721 gccacccagc cgaggacgag cgtgcccgcc ggcagaagcg cggcactccg gacgcccgcc
     3781 agctggaacg ccgtcagcgc gaacgcggtc gcgaggaggc ccagtaacgg gcggccgcgc
     3841 tggaagcgcc aggccaggaa tccgccgacc accaggacca ccggcggcag gatggccgcg
     3901 agagcctcga cgcccggcag ccgacccgca aaggcgacga cgacgccggt ggcaaggacg
     3961 acgatcccct cgaggccgat cagtccgtag aacgcgttca gcatatcctc gttcggcgtg
     4021 ggagcagggg cgagaagtga acggcgccga gccggcgaca caaggggcgc ggtcggccga
     4081 tcccgctaga tttggggata tgaagatccg gatcgtggtc gccgcgctgc tcatcgcgac
     4141 gccgctcgcg gcccaggccg gcgccgactc gatgaccaac gccggcgtgg tgcgcgccgc
     4201 ggacgcggtg gattcggtgt tcctggcccg cacccgcgta tcggacactg tcgccggcgg
     4261 cgactgggcc gcctacctga tggcgcgcct cggcgtcaag ccgatccccc cggacctccg
     4321 gctcaaggtc gccaccgact cggccgccat ccatatcttc ggccacattg ccgacctccc
     4381 ggcggaggcg cggatgcagc tcggcccgct cctctcgctc ctcgacacct cgacgaccat
     4441 cgatgcgtgg gcgtcgttgg cccaggcggg gcccgccgcg gtccggttcc ggctcgactc
     4501 ggcggccgtg ggcggaatgg gcatccccga ggtggcgctg gcccccgtcc tggccaacgt
     4561 cggcgtccgc tttcccgtgc tcacccggac cggccgcgat ctgttcgtcg agaccccgcc
     4621 atacggtcgg gtggaactgg cgttgaacgg cgtgctgctg gtggcgccgc cgccgccccc
     4681 gtccgcgccc cacacccgac cccgccggac gcgcgcccgg tgagcgaccc cgccagcctc
     4741 cgccgcatct accggctctg gcgcacttcg gccatcctct gcggcctcgg ggcggtggaa
     4801 ttcgccctgc tcgcctggca gcggggcgtg gcgtggatcg ctgccgcggc cgtcccgctc
     4861 gtggcggtct cgatctttgc cacccgccgc gccgcccggg cccgccggtc gtggggaatc
     4921 cggtagcacc gacctccacc ttccgccctg cgaccatgtc acccatcctg ctgcgtaccg
     4981 ccggcctcct cgtcctctcg aacgtgttca tgacgttcgc gtggtatgcc cacctccgga
     5041 acctcggcga ccggccggtc ttcgtcgcca tcctcgccag ctgggccatc gcgttcttcg
     5101 agtactgctt ccaggtgccg gccaaccgga tcggcttcac cgccttctcg ctggcccagc
     5161 tcaagatcct gcaggaggtc atcaccctcg gcgtgttcgt accgttcgcg ctcctctaca
     5221 tgaagcagcc gatcaagctc gacttcctct gggccggcct ctgcatggtg ggtgcggtct
     5281 acttcatgtt ccggggacac gttccggcct aggccggatt gcgccgcgcg gtacgccgtt
     5341 gcagtatgga tgggtcactc acgggggcac tcatgacagc attccaggct cgactcaagc
     5401 cgcaggtcgc ccagaagtac tccgggctca acagcgtcac ctggtacgac gtggagcccc
     5461 tctgggccgg cgtcaccgag cggaacatga acctcctcgg ccagcggatg acccggctca
     5521 ggaccgggaa ggaccacacg accgtcctgg cagaggacct cgaattccgc gagcaccccg
     5581 cccgggccac cgagcggacc acggaccggg cgaccgctca ggagtgaccc ccttgccacg
     5641 gcggaggggg ggtgcacaaa ttggccgcag ttcaccccgc ccccctgcgg ggcggcaggt
     5701 cccggcaggg cccaggaagg acgagatcgc ggtgccacgc aagaagaccc cggcgacaac
     5761 ggcaacaaag gccgccaaca atcccctcac cgacctgctc aaggtggaca acgccgagcg
     5821 gttctgggcg gggatcaacg acctcgtgtt cccgcgctat gagcggctcg ggtacgatgg
     5881 gctgtcgcag ggggagcagg tcgcgcactg catcgattgg ctggtgcggg aggtggaagc
     5941 gggaggcctt cgccagttcc tcagcgagat ctcaggcgac cacaccgcgg agaccctcac
     6001 cgcgctgcgc gccatcggcg cggaccacac cgccgagatg atggagcttg ccacgaccgt
     6061 cttcccggaa gaggtcccga tcgaccagga cgagcgcctg gccatcatgg aggacttcac
     6121 gggacgcgaa cgccggatgc tcaaggactt cgacgccggg ttcttcgaca actccgaaga
     6181 cctccttaag ctcctcaagg cctacatggc caagcacgtg gcggagttcc atccggagtg
     6241 agacgtgaga cgtgagacgt gagacgtgag acgtgagacg tgagacgtga gacgtgagac
     6301 gtccgaaggt accgaacacc tgccgatgcg aaaggggccc cgtcactcag cggagcccct
     6361 tttcacttct tacttctcac ttttcactag tacctgtagt gctccgcctt gtacggcccc
     6421 tccggcttca ccccgatgta cttcgcctgc gccggcgtga gggtggtgag cctgacgccg
     6481 agcttgtcga ggtggagccg ggccaccttc tcatcgaggt gcttgggcag ggtatagacc
     6541 ttcgtctcgt acttgcccgc gttctggtgc agctcgatct gggccatcac ctggttggtg
     6601 aagctggccg acatcacgaa gctcgggtgt cccgtggcgt tacccaggtt gaacagccgc
     6661 ccctcggaga ggatcagcac cgagtggccg tccgggaagg cccactcgtc gtactgcggc
     6721 ttgatgttga cccgccgcac gcccttgatg tccttgaggc cggccatgtc gatctcattg
     6781 tcgaagtggc cgatgttgcc gatgatcgcc ttgtccttca tccgcgccat gtgctgggcg
     6841 gtgatgatgt cgcgattgcc ggtggcggtg atgaagatgt cggccgtcga caccacgtcc
     6901 tccagcgtca gcacctggta cccttccatc gacgcctgga gcgcgcagat cgggtcgatc
     6961 tcggtgatga ccacccgggc gccctgcccc ttgagcgcct gggcgcaccc cttgcccact
     7021 tccccgtagc cgcacaccac cgccagcttc ccggcgatca tcacgtcggt ggcgcggttg
     7081 agtccgtcga tcaccgagtg ccggcacccg tagatgttgt cgaacttcga cttggtgacg
     7141 gagtcgttga cgttgatggc cgggaagagc agcgtgccgg cccgcatcat ctcatacagc
     7201 cggtggacgc cggtcgtggt ctcctcggag acgccacgga tcccggcggc gaccgtgccc
     7261 caccgccccg ggtgcgcctt cgcctcggcg cggagcaggt cgaggatgac cccccattct
     7321 tccgggtcgt tcgcggcgtc gaacgccggg atcttccccg accgttcgta ctccgccccc
     7381 ttgtgcacca gcagcgtggc gtcgccgccg tcgtcgagca gcaggttcgg gcccccgcca
     7441 tcgggccaca tcagcgcctg ctcggtgcac caccagtact ccagcagggt ctcgcccttc
     7501 cacgcgaaca ccgggacgcc ccgcgggtcg gattcggtgc cgtccttccc caccaccacg
     7561 gccgcggcgg cctggtcctg ggtcgagaag atgttgcacg acacccagcg cacgtcggcg
     7621 ccgagcgcgg ccagcgtctc gatcagcacc gcggtctggg tggtcatgtt gagggagccc
     7681 atgatgcggg cgccggcaag cggccgcttg ccggcgtatt ccgaacggag tgccatcagc
     7741 cccggcatct cctgttcggc cagccggatc tcgttgcgtc cccactccgc gagcgagagg
     7801 tcgcgcacct tgaagggcgg gcgatcggtg ctctcgctcg tggtacgggg ctcggttgtg
     7861 acggtggtgc tcatgactgc tccgaacggt tgaatgttat cggtgatt
//
LOCUS       DASUAP010000270         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1343216, whole genome shotgun sequence.
ACCESSION   DASUAP010000270 DASUAP010000000
VERSION     DASUAP010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343216"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VFU45_07165"
     CDS             <1..941
                     /locus_tag="VFU45_07165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5358880.1"
                     /translation="GVRAREERLHAQVDTLKREIDEAQRGSAAGVDAEPDAGTLIPGA
                     LFADRYTITRVVGRGGMGMVYKAHDRELDEDVAIKLLRPDVLRGDPTMVERFKSEIRL
                     ARRISHTNVVRTHDFGERDGTYYVTMEYVEGITLRELIELRSRLSAEATLGIARQLAV
                     SLAVAHEQGVVHRDLKPENLLLDPAGVLKVMDWGIAKLADRSSTITQAGMVVGTPAYM
                     APEQLLAETVDARSDLYAAGVVLYECLTGALPYEAGNPLTLIARVLNQVPAAPHERNA
                     EVPGPLSSLVMRLLAKDPAGRPQTAAELGELLAQIG"
     gene            1173..2585
                     /locus_tag="VFU45_07170"
     CDS             1173..2585
                     /locus_tag="VFU45_07170"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5358881.1"
                     /translation="MRRTLRASLLFVLLAAPTAVAAQGVNTLPPDLSAKVQAAVTDVM
                     TRTHVPSASVGIVQGGRIVYTAAFGSARLSPALPASPDMHYAIGSISKQFTTACVLLL
                     AQEGKLSLDDPVSKWFPELTRSSEVTIRNLLSHTSGYEDYAPQDYTIPAWTHPTTAQA
                     IIHEWATKPLDFDPGTQYQYSNTNFNIVGLIVEKASGQPFWQFLSSRVLKPLHLDHAI
                     DLDTQREQLEPLGYARAALGPLRPARMEAPGWYFADGEMAMPVGDLLTWDISLMDRTL
                     LSPASYDAMETEMKLKNGQGIGYGLGVAVGYRNGHRVVAHSGEVGGFVAQNIVFPDDR
                     IAIAVLTNQEASSAAGAIGQALAGVLLPGTPAAADPGPAEAQATAILGGLQQGKIDRS
                     LFTANANFYFDDTTLGDFKSSLGPLGAVKSVHQTRSALRGGMTYRGFAVEFANGTKVS
                     LSTYTTTDGKIEQFLVDTPG"
     gene            2614..>2890
                     /locus_tag="VFU45_07175"
     CDS             2614..>2890
                     /locus_tag="VFU45_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358882.1"
                     /translation="MDKPNRIAQVYGYAVCLVAIVVALITIGSIVNSAFERANPLESG
                     STFGPSLTSFDAYKATYRQTPATAGTAAAADTMPEAALRARYDALASD"
BASE COUNT          452 a         1084 c          953 g          401 t
ORIGIN      
        1 cgggggtccg ggcgcgggaa gagcggctgc acgcgcaggt ggacaccctc aagcgcgaga
       61 tcgacgaggc ccagcgcggg agcgccgccg gcgtggacgc cgaacccgac gccgggacgc
      121 tgatcccggg ggcgctcttc gccgaccgat acaccatcac ccgcgtggtg ggacgcggcg
      181 ggatggggat ggtgtacaag gcgcacgacc gggagctcga tgaggacgtg gccatcaagc
      241 tcctccgccc cgacgtcctc cgcggcgacc cgacgatggt cgagcgcttc aagtcggaga
      301 tccggctggc ccggcggatc tcccacacca acgtggtccg cacccacgac ttcggcgagc
      361 gcgacgggac gtactacgtc accatggagt acgtcgaggg gatcacgctc cgggagctga
      421 tcgagctccg cagccggctc tccgccgagg ccacgctcgg catcgcccgg cagctggcgg
      481 tgtcgctcgc ggtggcgcac gagcaggggg tggtgcaccg cgacctcaag ccggagaacc
      541 tcctgctcga cccggccggg gtgctcaagg tgatggactg ggggatcgcc aagctggccg
      601 accgctcgag caccatcacg caggcgggga tggtggtggg gacgcccgcg tatatggccc
      661 ccgagcagct cctggcggag accgtcgacg cgcggagcga cctgtacgcg gccggggtgg
      721 tgctgtacga gtgcctcacg ggcgcgctcc cctacgaggc cggaaatccg ctcacgctca
      781 tcgcgcgggt gctcaaccag gtgcccgccg ccccgcacga acggaacgcc gaggtcccgg
      841 ggccgttgtc gtcgctggtg atgcggctgc tcgccaagga tcctgccggc cggccgcaga
      901 ccgccgccga gctgggggag ttgctggcgc agatcgggtg aggggatgcg ggcggcgcat
      961 gcgccgcccc tgcggatcag catcttcgga gccgtgccgg tccccgacac tgatcaagcc
     1021 cttcagggcg cagtgccgag aggcggcttc agccgccggt aaccgggcgg cgcatgcgcc
     1081 gcccctgcgg atcgcgcgtg gcggtcatgc cacatagtca ccgcgcccgc gggcccgtat
     1141 attgtcgaag tccttcccca gcgagatccc tgatgcgccg caccctccgt gcctccctgc
     1201 tcttcgtcct cctcgccgcg ccgactgcgg tcgctgcgca gggggtcaac accctccccc
     1261 ccgacctctc ggccaaggtc caggcggcgg tgacggacgt gatgacccgc acccacgtgc
     1321 ccagcgcatc ggtcgggatc gtgcaggggg gccggatcgt ctacaccgcc gcgttcggca
     1381 gcgcccggct ctccccggcg ctccccgcct cgcccgacat gcactacgcc atcggctcga
     1441 tcagcaagca gttcaccacc gcctgcgtgc tgctcctggc gcaggagggg aagctctcgc
     1501 tcgacgaccc ggtctcgaag tggttccccg agctgacgcg ctccagcgag gtcaccatcc
     1561 ggaacctcct gtcgcacacc tcgggctatg aggactacgc gccgcaggat tacaccatcc
     1621 cggcctggac ccacccgacc accgcacagg ccatcatcca cgagtgggcc accaagccgc
     1681 tcgacttcga tcccggcacc cagtaccagt acagcaacac caacttcaac atcgtcggcc
     1741 tcatcgttga gaaggctagc ggccagccct tctggcagtt cctctcctcg cgcgtcctca
     1801 agccgctgca cctcgaccac gcgatcgacc tcgacaccca gcgcgagcaa ctcgagccgc
     1861 tgggttacgc ccgcgcggcc ctcggtcccc tccgccccgc ccggatggag gcgcccggct
     1921 ggtacttcgc cgacggcgag atggcgatgc cggtcggcga cctcctcacc tgggacatct
     1981 ccctgatgga ccggacgctc ctctcccccg cctcctacga cgcgatggag acggagatga
     2041 agctcaagaa cggccagggg atcggctacg gcctcggcgt ggccgtcggg taccgcaacg
     2101 gccaccgggt ggtggcgcac agcggtgagg tgggcgggtt cgtggcgcag aacatcgtct
     2161 tccccgatga ccggatcgcc atcgcggtcc tgaccaacca ggaggcgagc tccgccgccg
     2221 gcgccatcgg ccaggccctc gccggggtcc tcctccccgg cacccccgcg gccgccgacc
     2281 cgggacccgc cgaggcgcag gccacggcga tcctgggcgg gctccagcag gggaagatcg
     2341 accgctcgct cttcaccgcg aacgcgaact tctatttcga cgacaccacg ctcggcgact
     2401 tcaagtcgag cctcggtccg ctcggcgccg tcaagtcggt ccaccagacg cggtcggcac
     2461 tccgcggcgg gatgacgtat cgcggcttcg cggtcgagtt cgccaacggc accaaggtgt
     2521 cgctcagcac ctacaccacc accgacggga agatcgagca gttcctggtc gatacgccgg
     2581 gatgagcggc accgcatcct gagggatccg ggaatggata agcccaatcg catcgcccag
     2641 gtctacggct acgcggtctg cctcgtggcc atcgtggtgg cgctcatcac catcgggtcg
     2701 atcgtgaact cggccttcga gcgcgccaac ccgctcgaga gcggatccac attcggcccc
     2761 tcgctcacct ccttcgacgc ctacaaggcc acctaccggc agacgccggc cacggcgggc
     2821 acggccgccg ccgcggacac gatgcccgag gcggcgctgc gcgcgcgcta cgacgccctc
     2881 gccagcgacc
//
LOCUS       DASUAP010000271         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1193673, whole genome shotgun sequence.
ACCESSION   DASUAP010000271 DASUAP010000000
VERSION     DASUAP010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193673"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFU45_07180"
     CDS             complement(<1..579)
                     /locus_tag="VFU45_07180"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEU5358883.1"
                     /translation="MTASARLYINIDHVATLREARKTDEPDPIAAARVVEAAGADGIT
                     VHLREDRRHIQDRDVESLARTVRTVLNLELAASPAIVDIACRLRPFQATLVPERREEV
                     TTEGGLDVLRGGAALRTACQRLAAAGCRVSLFIDPALPAIDAARALGVPAIELHTGRY
                     AAAWRQGDAALDELRRAAAHARAQGLAVHAGHG"
     gene            795..1262
                     /locus_tag="VFU45_07185"
     CDS             795..1262
                     /locus_tag="VFU45_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358884.1"
                     /translation="MTSRSVLGILAVAFLGACGGHSGGAVPGTVPPASSASGAVKEFM
                     AGVADSNIVRIGSAWGTEKGSAAKLNTPDDWQKRVTVMQLWLRGGTYTITGDQSLVND
                     RTHRQVTIDLVRSGCTRTIPITVVRSGGGWLVESIDLTAAGNPEKPCGDTPSK"
     gene            complement(1411..1806)
                     /gene="mce"
                     /locus_tag="VFU45_07190"
     CDS             complement(1411..1806)
                     /gene="mce"
                     /locus_tag="VFU45_07190"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEU5358885.1"
                     /translation="MTRAPEIAHVGVAVPDLDAALRFYRDVLGMTPHPPEVADGATIV
                     SLSFGGSEVELLAPRDAGSPVARFLEKRGPGIHHICYRVPDLTAALARCRQAGYQLID
                     EVPRTGAHGKRIAFVHPKSTAGILLELTE"
     gene            1868..2260
                     /gene="trxA"
                     /locus_tag="VFU45_07195"
     CDS             1868..2260
                     /gene="trxA"
                     /locus_tag="VFU45_07195"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5358886.1"
                     /translation="MTGPATLHEGAAGRQARERTRNMGNLTHVTDDTFGPVVEQGKGL
                     VLVDFWATWCGPCLAVAPVLEQLAGEYAGKARITKVDVDQNQKTAMRFNIRSIPSILF
                     FKDGKHVDTVVGAVPKPQLDAKIKQHLA"
     gene            2314..>2903
                     /locus_tag="VFU45_07200"
     CDS             2314..>2903
                     /locus_tag="VFU45_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358887.1"
                     /translation="MRASSARLLQTSVRLFDLVFPTTGAMITRTRIAFIHLDNVMSFA
                     KRDRDGQVDAWLAGYLPDELVLLFFRRGEVVNAASLRSDGRAVIPIAEALRRIQAEPE
                     RGELSFGRAPFEQLAWMFAACAGPARVRFVDEHQPGELFKVFATEGFNGVVEFISNGR
                     VNYFRFSDGKYAGGYCCDRPEGMPVAAYVESLFKPGP"
BASE COUNT          437 a          974 c         1063 g          429 t
ORIGIN      
        1 cccgtgcccg gcgtggaccg cgagcccctg ggcccgcgcg tgggccgccg cgcggcggag
       61 ctcgtcgagt gcggcatcgc cctggcgcca ggccgccgcg tagcgtccgg tgtggagctc
      121 gatcgcgggc acgccgaggg cacgggcagc gtcgatggcg gggagcgcgg ggtcgatgaa
      181 gagtgagacg cgacaccccg ccgccgccag gcgctggcag gcggtgcgaa gggcggcgcc
      241 gccgcgcagg acgtcgaggc cgccctcggt ggtgacctcc tcccgccgct cggggacgag
      301 tgtcgcctgg aacgggcgga gccggcaggc gatgtcgacg atcgccgggc tcgcggcaag
      361 ttcgaggttg agcaccgtcc gtaccgtgcg cgccagggac tcgacgtccc ggtcctggat
      421 gtggcgccga tcctcccgca ggtgcacggt gatgccgtca gcgccggcgg cctcgacgac
      481 ccgcgccgcg gcgatcgggt cgggctcgtc ggtcttgcgc gcctcgcgca gcgtggcgac
      541 gtggtcgatg ttgatgtaga ggcgggcaga tgcggtcatt ggtcgttggt cattcgagtc
      601 gttggtcctt taccgtcggg ccgggggaac gttagcgggg cggaagatcg ggcgtaatgg
      661 atgtaactct gccgcgtccc gaggcccaac gaccattggc caacgaccaa cgaccaacga
      721 ccgctgcggc gattgattgc ccccccgtaa ctcattacct tctaacacat tcccccatcc
      781 ggagcggatc gaccgtgacc tctcgcagcg tgctgggcat ccttgccgtg gcatttctcg
      841 gggcgtgcgg cggccacagc ggtggcgccg ttcccggtac cgtcccgccg gcctcgtccg
      901 catccggcgc cgtgaaggag ttcatggccg gcgtcgccga cagcaacatc gtccggatcg
      961 gcagcgcctg gggcacggag aaggggtcgg ccgccaagct caacacaccc gacgattggc
     1021 agaagcgggt gacggtcatg cagctctggc tgcgcggcgg cacgtacacc atcaccggcg
     1081 accagtcgct ggtgaacgac cggacgcacc gtcaggtgac catcgacctg gtccggagcg
     1141 ggtgcacccg gaccatcccg atcacggtgg tgcgctcggg cggcgggtgg ctggtggaat
     1201 cgatcgacct gacggcggcc ggcaatccgg aaaagccctg cggcgacacc ccgtccaagt
     1261 aggggccggg aaccggtcgg tgtttaggta gtaggtgctg ggaattaggt gccaggtgct
     1321 ccgtgctcga tccggctcca tcggcggcat cacccttcgg aacgtggcgc gtcactgagc
     1381 acctagcacc tggtacctgg cacccagcac ctactcggtg agctccagca ggatgccggc
     1441 ggtgctcttc ggatgtacga aggcgatgcg cttcccgtgg gcccccgtcc ggggcacctc
     1501 gtcgatgagc tggtagccgg cctggcggca ccgggccagc gccgcggtca ggtcggggac
     1561 gcggtaacag atgtgatgga tcccggggcc gcgcttttcc aggaacctgg ccaccggact
     1621 gccggcatcg cgtggagcga gcagctcgac ctcgcttccg ccgaagctga gcgagacgat
     1681 ggtggcgccg tcggccacct cgggaggatg gggtgtcatc cccaggacgt cgcgatagaa
     1741 gcgcagcgcg gcgtcgaggt cggggacggc gacaccgacg tgggcgatct cgggggcacg
     1801 ggtcacggct cgggtctcca gggcggcggg gcgggggccg cacggcaccc cacccgacgc
     1861 gacaacgata accggcccgg ccacgctcca cgagggcgcc gcgggccgcc aagcacggga
     1921 acggacaagg aacatgggaa acctgacgca cgtcactgac gacactttcg gcccggtggt
     1981 ggaacagggc aagggcctcg tgctcgtgga cttctgggcc acgtggtgcg gcccctgcct
     2041 ggcggtggcg ccggtgctgg agcagctggc cggggagtac gccggcaagg cgcggatcac
     2101 caaggtcgac gtcgaccaga accagaagac cgcgatgcgg ttcaacatcc gctcgatccc
     2161 gagcatcctg ttcttcaagg acgggaagca cgtggacacg gtggtcgggg cggtgccgaa
     2221 gccgcagctc gacgcgaaga tcaagcagca cctggcctga cccgggccat cacggcgcgg
     2281 cgtcccctcg ccgcgccgcc cttcgagggc cccatgcgcg cgagcagcgc ccggctcctg
     2341 cagacatcgg tgcgtctctt cgacctcgtc ttcccgacga cgggcgcgat gatcacgcgc
     2401 acgcggatcg ccttcatcca cctcgacaac gtgatgtcgt tcgccaagcg cgatcgcgac
     2461 ggccaggtcg acgcgtggct ggcgggttac ctccccgacg agctggtcct gctcttcttc
     2521 cgccggggcg aggtggtcaa cgccgccagc ctccggtccg acggccgcgc ggtgatcccc
     2581 atcgccgagg cgctgcgccg gatccaggcg gagccggaac ggggggaact gagcttcggc
     2641 cgtgcgccgt tcgagcagct ggcgtggatg ttcgccgcct gcgccggccc ggcgcgggtc
     2701 cggttcgtgg atgagcacca gccgggcgag ctcttcaagg tcttcgcgac cgaggggttc
     2761 aacggcgtgg tggagttcat ctcgaacggc cgggtcaact acttccgctt cagcgacgga
     2821 aagtacgccg gcggctactg ctgcgaccgc cccgagggga tgccggtggc ggcgtatgtc
     2881 gagtcgctgt tcaagccggg ccc
//
LOCUS       DASUAP010000272         6505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_8790, whole genome shotgun sequence.
ACCESSION   DASUAP010000272 DASUAP010000000
VERSION     DASUAP010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8790"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFU45_07205"
     CDS             complement(<1..599)
                     /locus_tag="VFU45_07205"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5358888.1"
                     /translation="MPQLPTLSVIVPVYNEIRTLEAVVARLHSVPIPMEIICVNDCSR
                     DGSGELMDRLKAAGQIQLAVHHPVNRGKGAALRSGIQHATGDVIVVQDADLEYDPEDF
                     PKLLGPIIAGKADAVFGSRFLGAEHRVLYYWHSVGNSLLTTLSNMFTNLNLTDMETCY
                     KMVRAPLMKSLIMTSDRFGFEPEITARLAQSGARIWEVPV"
     gene            complement(688..2076)
                     /locus_tag="VFU45_07210"
     CDS             complement(688..2076)
                     /locus_tag="VFU45_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5358889.1"
                     /translation="MSQKPCVLVIDDEMGILDTLRILLKGEGFDVITAQGGKAGIEQI
                     KLGQHDLILSDVRMPQVTGLDILQAAKEQDPMTPVILMTAQASLQTAMSALNQGAFYY
                     LQKPFANDELLALLRRAVDFRAVRAENKQLKQDIRRRERTAPARPLGKARRFVEVLKL
                     TEMVAPTEATVLIQGESGTGKEVLARYVHNLSPRREGPFLSINCGALPENLLESELFG
                     HVKGSFTGAVRDKQGLFAAARGGSFFLDEVGEMPASLQVKLLRVLQEREAIPVGGTEP
                     VPLDVRIIAATNRDLEDEVRRGAFRSDLFYRLNVFALSLPPLRERPEDLMALVEFFLQ
                     RMAADRGIDAKALSPDAIDAVMVYDWPGNVRELENAIEHALVVTKGDAIDLGALPERI
                     TRRRHETLLSERAHANPTLETIERAYIMWVLQSEGGNKTRAAEVLGIDPSTLYRKLSR
                     YEGVEGVAGRGA"
     gene            complement(2073..3755)
                     /locus_tag="VFU45_07215"
     CDS             complement(2073..3755)
                     /locus_tag="VFU45_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5358890.1"
                     /translation="MTSPQPSLASARASIWQHLPSVGTVLTGLLGLRVMVATFALWRA
                     ALMWDEDPSIAFVVSIMVLIVYVLTFYGFWVVHGRRAAQPTTSQLYAQAVADLLAATT
                     LVHVSGGDRSAFSAIYVLLIATYTLLMSFGAGLLITVLSISVYLADIFWGQPVPPDTT
                     FIGQAVVFGLVFAVVSGLASRLKEEGRKAASLQTELARVRLEADDILRTITSGVLTVD
                     ALGRLVFINPMAERMLGLPAREYLGRPALDTIRSAAPELGQAILSVLRAGRRVSRAEG
                     TIRAAGREFPVGLSVTTLERDREGAPPSVTAIFADISESKRLQELHLRAERLEAVAEL
                     SASLAHEIRNPLASIRSSVEQLSRSSRASDDERFLAQLIMRESDRLSRLLSEFLDFSR
                     VRITRSEPLDLLQVARSAARLVREHPDCREDVTITVDGETAELDGDEDLLHRVVANLL
                     LNAVQAAKGAVTVHVNCRPAAAAELPSGSGLERAVRLEIRDNGPGIPAEVRERLFEPF
                     VTGRVGGSGLGLSIVQRAVEAHRGLVLVDTGAGRGTTFTIYLPVHWATEEAA"
     gene            4025..4993
                     /locus_tag="VFU45_07220"
     CDS             4025..4993
                     /locus_tag="VFU45_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02453 family protein"
                     /protein_id="HEU5358891.1"
                     /translation="MPWHVYIVRCGDGTLYTGVATDLARRVAAHDAGRGARYTRSRRP
                     VTLAWSTTARDRSAAQRLEAALKKLPRPAKERLIREGTTSTNEWHGLPKAGLTFLRQL
                     KRNNRREWFEEHRATYDAALREPMRALVEAMDVRFAGFAAEIVGDPKRSLFRIHRDVR
                     FARDKSPYKTHAALWFAHRDAGFGVGREAHGGAGFYFHVEPGSCYVAGGFWMPPRPAL
                     DRIREALAEDPGSFARIVDAPAIRRTFGPLSTEGMLKRLPRGTAPGHPAERWLRHQSF
                     TVSTELPESAVFRRDLPALLERRYRTLLPLVRWLNSAIGLQAAARR"
     gene            complement(5243..>6505)
                     /locus_tag="VFU45_07225"
     CDS             complement(5243..>6505)
                     /locus_tag="VFU45_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU5358892.1"
                     /translation="AAGAAVYRSLVNDRVLGARALYEEGRALLRLPDHTAGIRVLEAV
                     VRRHRGDTTVAASALFLLGGEDADRGDDRGARRRWGELAHRYPSDRLAPAARFQADVI
                     SFVRGEFTRAARGFDSLRAPGTGAAEANAAGYWAGRAWAAAGKPDTASARWHEVMDQS
                     PESYYAGASAARLGIVAPPIHEPGPPAPVPEFIARAERQIALLDATGLDLEARWVREM
                     LGAAAGGPDTLIAAAGVLLQEDAPAPAGRLARAALLKPGVDSARALRLLYPLDARDAL
                     SREAARRDLDPAFVAALIRQESGFDPEAVSAAGARGMMQVMPTLGAALARRLRFPLWD
                     PVLLFQPDVSLEIGMAQLQNLFDRFRDPARVLAAYNAGGANVLAWEQRAGVRDPDVFI
                     ERIPFPETRDYVRTIIRNREMYRRLYGW"
BASE COUNT          939 a         2298 c         2349 g          919 t
ORIGIN      
        1 accggcacct cccagatccg ggcgccggac tgggccaggc gggcggtgat ctcgggctcg
       61 aagccgaagc gatcgctcgt catgatgagc gacttcatca gcggggcacg gaccatcttg
      121 tagcaggtct ccatgtcggt caggttcagg ttggtgaaca tgttcgacag ggtggtgagc
      181 agcgaattgc ccaccgagtg ccagtagtag agcacgcgat gttccgcgcc gaggaagcgg
      241 gagccgaaca ccgcgtcggc cttgccggcg atgatcggac ccaggagctt ggggaaatcc
      301 tccggatcgt actcgaggtc ggcgtcctgg accacgatga cgtcgccggt ggcgtgctgg
      361 atgccggagc ggagcgcggc gcccttgccc cggttgaccg ggtggtggac cgcgagctgg
      421 atctgccccg ccgccttgag ccggtccatc agctcgccgc tcccgtcccg ggagcagtcg
      481 ttgacgcaga tgatctccat cgggatcggc accgagtgga ggcgcgccac gaccgcctcc
      541 agcgtgcgga tctcgttata gaccgggacg atcacggaca gggtgggaag ctggggcata
      601 tcggcctggg tgtggttgta gcggctgcgg gcctcggggt ccgctatccg ctatccgacc
      661 tgcgactcta gactcgcgac tcacgactca cgccccgcga cctgcgaccc cttccactcc
      721 ctcgtaccgg ctcagcttgc ggtacaacgt ggacgggtcg atccccagca cctcggcggc
      781 gcgggtcttg ttgccgccct ccgactggag cacccacatg atgtatgcgc gctcgatggt
      841 ctcgagggtc gggttggcgt gcgcccgctc gctgaggagg gtctcgtggc ggcggcgggt
      901 gatgcgctcc gggagcgcgc ccaggtcgat cgcgtcgccc ttggtcacga ccagcgcatg
      961 ctcgatggcg ttctcgagct cccgcacgtt gcccggccag tcgtagacca tcacggcgtc
     1021 gatggcgtcg ggcgagagcg ccttggcgtc gatgccgcgg tcggcggcca tccgctgcag
     1081 gaagaactcg acgagcgcca tcaggtcctc cggccgctcg cgcagcggcg gaaggctgag
     1141 cgcgaagacg ttgagccggt agaagaggtc ggaccggaac gcgccacgcc ggacctcgtc
     1201 ctcgaggtcg cggttggtgg cggcgatgat ccggacgtcg aggggcacgg gttccgtgcc
     1261 gccgaccggg atggcctcgc gctcctgcaa cacgcgcagg agcttgacct gcagcgaggc
     1321 gggcatctcg cccacctcgt cgaggaagaa gctcccgccc cgggccgcgg cgaagagccc
     1381 ctgcttgtcg cgcacggcgc cggtgaagga ccccttcacg tggccgaaga gctcgctctc
     1441 gaggaggttc tcggggagcg cgccgcagtt gatggagagg aacggcccct cccgccgggg
     1501 cgaaaggttg tggacgtagc gggccaggac ctccttgccg gtgccgctct cgccctggat
     1561 gagcacggtg gcctcggtcg gcgccaccat ctcggtgagc ttgagcacct cgacgaaccg
     1621 ccgggccttg ccgagcggcc gggccggcgc ggtccgctcg cggcggcgga tgtcctgctt
     1681 gagctgcttg ttctccgccc gtacggcgcg gaagtcgacc gcgcggcgga gcagcgccag
     1741 cagctcgtcg ttggcgaagg gcttctgcag gtagtagaac gcgccctggt tgagggcgga
     1801 catggcggtc tggagcgacg cctgggcggt catcaggatg accggcgtca tcgggtcctg
     1861 ctccttggcc gcctgcagga tgtcgaggcc ggtgacctgg ggcatccgca cgtcagagag
     1921 gatcaggtcg tgctggccca gcttgatctg ctcgatgccg gccttgcccc cctgggcggt
     1981 gatgacgtcg aacccctcgc ccttgaggag gatccgcaag gtgtcgagga tccccatctc
     2041 gtcgtcgatg accagcacgc acggcttctg gctcacgccg cctcctcagt cgcccaatgc
     2101 accggcaggt aaatggtgaa cgtcgtgcca cggccggcgc cggtgtccac cagcacgagc
     2161 ccgcgatggg cctcgaccgc ccgctggacg atcgagagcc cgagccccga gccgccgacc
     2221 cggccggtga cgaacggctc gaagagccgc tcccgcacct ccgccgggat gccgggcccg
     2281 ttgtcgcgga tctccagccg caccgcccgc tcgagcccgc tcccggaagg tagctccgcc
     2341 gccgccgcgg ggcggcagtt cacgtgcacg gtcacggcgc ccttggccgc ctgcacggcg
     2401 ttgaggagga ggttggccac cacccggtgg agcaggtcct cgtcgccgtc gagctcggcc
     2461 gtctccccgt ccaccgtgat ggtcacgtcc tcccggcagt cggggtgctc gcgcaccagc
     2521 cgcgcagccg agcgcgccac ctggaggagg tcgagcggct ccgaacgggt gatgcgcacc
     2581 cgggaaaagt cgaggaactc gctcagcagc cggctcagcc ggtcgctctc ccgcatgatg
     2641 agctgggcca ggaagcgctc gtcgtcgctc gcgcgggagg agcgggagag ctgctcgacc
     2701 gagctccgga tcgacgcgag cggattgcgg atctcgtgcg ccagcgatgc actcagctcg
     2761 gccaccgcct cgagccgctc ggccctgagg tgcagctcct gcagccgctt cgactcggag
     2821 atgtcggcaa agatggccgt caccgacggc ggcgcaccct cgcgatcgcg ctcgagcgtg
     2881 gtgacgctca agcccaccgg gaattcgcgc ccggcggcgc ggatcgtccc ctccgcccgg
     2941 ctcacgcggc ggccagcgcg gagcaccgag aggatggcct ggcccagctc gggggccgcc
     3001 gaccggatcg tgtcgagggc gggccggccc aggtactccc gggccggcag gcccagcatc
     3061 cgctcggcca tcgggttgat gaacacgagc cggccgagcg cgtcgacggt caggaccccg
     3121 ctcgtgatgg tgcgcaggat gtcgtccgcc tcgagccgga cccgggccag ttccgtctgg
     3181 agcgaggccg ccttccggcc ctcctccttc aggcgcgagg caaggccgct cacgaccgcg
     3241 aacaccagcc caaagaccac cgcctggccg atgaacgtcg tgtccggcgg caccggctgg
     3301 ccccagaaga tgtcggccag gtacacgctg atcgacagca ccgtgatgag gaggccggcg
     3361 ccgaaggaca tcagcagcgt atacgtcgcg atcaggagga cgtagatggc ggagaaggcg
     3421 ctccggtcgc caccgctcac gtgcaccagg gtcgtcgcgg ccaggaggtc ggcgacggcc
     3481 tgcgcgtaga gctggctcgt ggtgggctgg gcggcccggc gcccgtgtac cacccagaac
     3541 ccgtagaacg tcagcacgta gacgatcagc accatgatgc tgacgacgaa ggcgatgctc
     3601 gggtcctcgt cccacatcaa cgcagcgcgc cagagcgcaa aggtcgcgac catcaccctg
     3661 aggccgagca accccgtcag caccgtcccg accgagggaa ggtgctgcca gatggaggcg
     3721 cgcgcggagg ccagcgatgg ctggggcgag gtcacaagcc ttggggtcgg ggacgccggc
     3781 ccggagcccg ggcgcggtcg gcaactgcgc tggaatatgg aaggcccgcg ggcgggcggg
     3841 aaggcggggg aaagcgaagg aaggcggagg aaagcgggaa aagcggggga aggcgtggga
     3901 acgcggggga aggcggggga aagcgaggga aagcctggga gagcgtggga aggcgggacc
     3961 ccgggacgtg cctcagctcg cgccctcttc acttttcact cttcacgctt cactgttcgt
     4021 cccgatgccc tggcacgtct acatcgtccg ctgcggcgac gggacccttt acaccggcgt
     4081 cgcgacggac ctcgcccgcc gggtggcggc gcacgacgcg ggccggggcg ccaggtacac
     4141 ccggtcccgg cggccggtga cactggcctg gagcaccacg gcgcgcgacc ggtcggctgc
     4201 ccagcggctc gaggcggcgc tcaagaagct gccgcgcccg gcgaaggagc gcctgatccg
     4261 ggaggggacc acgtcgacga acgaatggca cggcctgccc aaggcggggc tcaccttcct
     4321 gcggcagctc aagcggaaca accgtcgcga gtggttcgag gagcaccgcg ccacctacga
     4381 cgccgcgctg cgggagccga tgcgggccct ggtcgaggcg atggacgtgc gctttgccgg
     4441 cttcgccgcc gagatcgtcg gcgacccgaa gcgctcgctc ttccgcatcc accgggacgt
     4501 ccgcttcgcc cgcgacaagt ccccgtacaa gacccacgcg gcgctctggt tcgcgcaccg
     4561 cgacgcggga ttcggcgtgg ggcgggaggc ccacggcggc gccggcttct acttccacgt
     4621 cgagccgggc agctgctacg tggccggcgg cttctggatg cccccccgcc ccgcgctcga
     4681 ccggatccgg gaggcgctgg cggaggatcc ggggtcgttc gcgcggatcg tcgacgcgcc
     4741 cgcgatccgc cgcaccttcg gcccgctcag cacggagggc atgctcaagc ggctcccccg
     4801 tggcacggcg cccggccatc ccgccgagcg gtggcttcgg caccagtcgt tcaccgtgtc
     4861 gaccgagctc cccgagtcgg cggtcttccg ccgcgacctc cccgccctgc tcgaacgccg
     4921 ctaccggacc ctcctgccgc tggtgcgctg gctcaactcg gcgatcgggc tgcaggcggc
     4981 ggcgaggagg taggaccgat cggaggatgg gactgggcga tgctcggcgc acggagcccg
     5041 gcgcgcaaga ggtgtcatcc tgagcggagc gaaggatctc tttgcgtcca cccgagagat
     5101 ccttcggtcg ctttgctccc tcaggatgac tccgcgggct cagctacctc cgcttccccg
     5161 cccgccgctt ccctcgcctc atcacctcct cgcctcttcc cttccctctc ctgcttcctc
     5221 ttcccttctc acttttccct cctcaccacc cgtacagcct ccgatacatc tcccgattgc
     5281 ggatgatggt ccggacgtag tcccgggtct cggggaacgg gatccgctcg atgaacacgt
     5341 cgggatcccg gaccccggcc cgctgttccc acgccagcac gttggcgccg ccggcgttgt
     5401 acgcggccag gacgcgggcg gggtcgcgga agcggtcgaa gaggttctgt agctgggcca
     5461 tcccgatctc gaggctgacg tcgggctgga agaggagcac cgggtcccag agcgggaagc
     5521 ggagccggcg ggccagcgcc gcgccgagcg tcggcatgac ctgcatcatg ccgcgggcgc
     5581 cggccgcgga gacggcctcg ggatcgaatc ccgattcctg gcggatgagc gcggcgacga
     5641 acgcgggatc gaggtcgcgg cgggccgcct cgcgcgacag agcgtcccgg gcgtcgagcg
     5701 ggtagaggag ccggagcgcc cgcgccgagt cgacgccggg cttgaggagc gccgcgcgcg
     5761 ccagccgtcc cgcgggcgcc ggcgcatcct cctgcagcag gacgccggcc gccgcgatca
     5821 gcgtgtccgg gccccctgcg gcggcgccca gcatctcacg cacccatcgg gcctcgaggt
     5881 cgagtccggt ggcatcgagc agcgcaatct ggcgctcggc gcgggcgatg aactccggca
     5941 ccggcgcggg cgggcccggc tcatggatcg gcggcgccac gatccccagg cgcgcggcgc
     6001 tcgcgccggc gtagtacgac tcgggactct ggtccatcac ctcgtgccag cgggcgctcg
     6061 cggtgtcggg cttcccggcg gcggcccagg cccgccccgc ccagtatccg gcggcgttcg
     6121 cttcggcggc gccggtcccg ggggcccgga gactgtcgaa gccgcgcgcg gcccgggtga
     6181 actcgccccg gacgaagctg atcacgtccg cctggaaacg ggccgccggc gccagtcggt
     6241 cgctcggata ccggtgcgcc agttcgcccc agcggcggcg ggcgcctcgg tcgtcgccgc
     6301 ggtcggcgtc ctcgccgccc agcaggaaga gcgccgacgc ggcgacggtc gtgtcgcccc
     6361 ggtgccggcg gaccactgcc tcgagcacgc ggatccctgc cgtgtggtcg gggaggcgga
     6421 ggagggcccg gccttcctcg tacagcgccc gcgcgccgag cacccgatcg ttgacgagcg
     6481 accggtatac cgccgctccg gcggc
//
LOCUS       DASUAP010000273         8073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_948718, whole genome shotgun sequence.
ACCESSION   DASUAP010000273 DASUAP010000000
VERSION     DASUAP010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948718"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2042
                     /locus_tag="VFU45_07230"
     CDS             <1..2042
                     /locus_tag="VFU45_07230"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEU5358893.1"
                     /translation="LGNIGPLAGKPVMEGKGVLFKAFADLDVFDLEIDALKPDDVIRF
                     CQMLAPTVGGINLEDIRAPDCFAIEEALRETLDIPVFHDDQHGTAIISGAALLNALEV
                     TERDITTTRVTFVGAGAAAIATAELYVKLGVPRENITLCDKGGVVYEGRGEMDAYRAR
                     FARKTEARSIADAFVGADVFVGLSAAGTVTPEMVKTMAPRPIIFALANPTPEIMPDVA
                     RAARPDAVIATGRTDFPNQVNNVLGFPFIFRGALDVRARKINEEMKMAATRALAALTK
                     EDVPDSVLKAYGLDSLRFGPDYLIPKPFDPRVLLWVAPAVAWAAIASGVAREIIDLDE
                     YRAKLESRQGRGRAIIRGLINRAVAEPKRLAFTDAEDTRVIRAARIVADEGIGSPVLL
                     GDEREVFEAATEAGIPLNNIEVLDPARSAQREAYATAFWKRRQRKGVTRGEARRRMAD
                     PAYYGAMMVSQGHADALVAGQAQYYPDAIRPALEVVGVLPGGAVAGIYMMIVGVKTFF
                     FADCTVNIDPDAARLAEIAEAVAGFVKHFGIDPRVALLSFSNFGSVKHPAQVKVAEAV
                     RLLHQRLPGLPVDGEMQADTAVVESILQQTYAFSRLQGPANVLIFPDLNSANISYKLL
                     ARLGGAQAIGPILVGLAAPIHVLQRGADVDEIVNMAVIAAVDAQERSRRAATVAR"
     gene            2068..3663
                     /locus_tag="VFU45_07235"
     CDS             2068..3663
                     /locus_tag="VFU45_07235"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEU5358894.1"
                     /translation="MTATKPAPEAIGIPGTGLHPIRVHANLHPPQLIEHAVRRGEGTL
                     TAHGAHTTITSPHTGRSPNDKFVVDEASCTAEIWWDKNGKGDEAGFEKLLQAVRAHLD
                     AQEVFVQDLYAGADRSHRLNVRFVTPNAWQALFVRNMFIRPDAADLAGFAPGFTVLHA
                     PELQADPAVHKTRTGTFVILHFARKLVLIGGTRYAGELKKSIFTVLNYLLPRQGVLPM
                     HCSANIGPDGDTAIFFGLSGTGKTTLSADPARHLIGDDEHGWGEHGVFNFEGGCYAKV
                     IRLSAEGEPEIYAASQRFGTILENVVVDPATRLPDYNDQSITENTRSSYPIDYIPNHE
                     PSGQGGHPRHIVFLTADAYGVLPPIARLSPEQAMYQFVSGYTAKVAGTERGVTEPTAT
                     FSACFGAPFLPLHPGVYAKMLGQKIAKHGATCWLVNTGWTGGAYGTGSRMKLAHTRSM
                     VRALLAGKLAKAEFRTDPVFGFDVPTAVPDVPAAVLNPRETWADKAAYDAQAKKLAQM
                     FRENFAKFADHVEAKVQRAGPTV"
     gene            3857..4270
                     /locus_tag="VFU45_07240"
     CDS             3857..4270
                     /locus_tag="VFU45_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5358895.1"
                     /translation="MALTIGRVAAEVGVNIQTIRYYERRGLLVPPDRSASGYRQYAGD
                     AVSRLRFIKHAQELGFSLAEVRGLLDLRVRPGAACDAVEQSTRKQIDRVQGKIRSLQR
                     IERTLERLATACEARQATDACPILEALEDHANTND"
     gene            4251..4604
                     /locus_tag="VFU45_07245"
     CDS             4251..4604
                     /locus_tag="VFU45_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
                     /protein_id="HEU5358896.1"
                     /translation="MPTPTTKTLLGATGGVAAAVASALCCVGPLVAVALGLSGAGLAA
                     TFEPLRPYFIAGTVAALGLGFTTLWREERRACEPGTPCASPAARRRMKRLLWVATIIA
                     VSFLTFPWWSKYLLG"
     gene            4614..5000
                     /locus_tag="VFU45_07250"
     CDS             4614..5000
                     /locus_tag="VFU45_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEU5358897.1"
                     /translation="MIASIRTALVLVAAASGSTLLCPRGGECALPAASNLARIATQAT
                     DTATVQLHISGMTCGSCPITARIALRKLPGVFSATVTLDDSLGVVRYDPSKVTPAQIA
                     AHLTRLTGYGAKVIADGAKALRQGGK"
     gene            5224..6873
                     /gene="merA"
                     /locus_tag="VFU45_07255"
     CDS             5224..6873
                     /gene="merA"
                     /locus_tag="VFU45_07255"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HEU5358898.1"
                     /translation="MRELTIEIRGMTCDHCERAVATALHAVPGVRRVDEVNHVRGFAR
                     VTAGPEATSARIEDAIAKAGYRALVRPEPTGTVASTTIGQQAGEFDLTILGGGSAGFA
                     AAIRAAELGARVAMVEAGTLGGTCVNVGCVPSKTLIRAAEMQHRRLHHGFQGIPITGG
                     SVDWNLVRTQKDELVAGLRQAKYWDVLHAYPSITLFEQQAEIASGGGVRLADGRIITS
                     GKILVTTGASPWAPPIPGLADAGHLDNASAMALPELPNSLIVIGASAVGLELAQMFAR
                     LGVTVTVVEAQPRVVPGEDPDVGDALGASLEREGLDLRIGMQIERVGRDGSDYTVSFK
                     GGGSVRAEQLLVATGRRANVAGLGLGAAGVALGKKGEIVVDQFLQTANPGIYAAGDVT
                     GDPMFVYVAAYGGTIAAENALRGNARRYDLAALPKVTFTDPAVASVGLTADQARAQGI
                     GPLVSLLPMEHVPRSLAARDTRGFIKLVADAATRKIVGAHILAAEAGEMITEPAMAIK
                     FGLTIDDLTSTFHPYLTLSEGIKLAAQTFDRDVAKLSCCAA"
     gene            6894..>8073
                     /locus_tag="VFU45_07260"
     CDS             6894..>8073
                     /locus_tag="VFU45_07260"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5358899.1"
                     /translation="MRPQLDHNMPNSPADFEIVRDPLWDNIRLDRDVATLVDTPAVQR
                     LRYVRQLGHAFLVYPGATHSRFEHALGTYHLARSALSALDERGELAGIGDDERLAVEL
                     AALLHDIGHYPFSHALEEAGFLDHESLGEAKLRHGELGDALRALGGDALITRIGRIIR
                     GRSDSPLAGLISGSLDLDKIDYLSRDARMCGVPYGTVDVSRLLAALTLVETGDGRREA
                     GVHDKGVSALESLLFAKYQMYRNVYWHHAVRAATCMFKRAVGAAVIAGALTPDAIADA
                     TDASLGEALLRHDPTGLARAVHGRRLHKRAADLAAADLPADTQPWISDDPRLVERVED
                     ALAIELGLPAGGLLLDFPSRSTMLSVDLPLRTRTGETARLTDAGRAGQLGLPRVAEEL
                     Y"
BASE COUNT         1272 a         2850 c         2738 g         1213 t
ORIGIN      
        1 ggctcggcaa catcggtccg ctggccggca agccggtgat ggagggcaag ggggtcctgt
       61 tcaaggcgtt cgcggacctc gacgtcttcg acctcgagat cgacgccctg aagccggacg
      121 acgtgatccg gttctgccag atgctggccc cgacggtggg cgggatcaat ctcgaggaca
      181 tcagggcgcc cgactgcttt gccatcgagg aggcgctccg ggagactctc gacatcccgg
      241 tcttccacga tgaccagcac ggcaccgcca tcatctcggg cgcggcgctc ctcaacgcgc
      301 tcgaggtgac cgagcgggac atcaccacga ccagggtgac cttcgtcggc gccggcgccg
      361 ccgccatcgc gacggcggag ctctacgtga agctcggcgt cccgcgggag aacatcacgc
      421 tctgcgacaa ggggggcgtg gtgtacgagg gccggggcga gatggacgcg tatcgggccc
      481 gctttgcccg gaagaccgag gcccggagca tcgccgacgc cttcgtcggg gccgacgtct
      541 tcgtgggcct gtcggccgcg gggacggtga cgcccgagat ggtcaagacg atggcccccc
      601 ggccgatcat cttcgccctg gccaacccga cacccgagat catgcccgac gtggcgcggg
      661 cggcgcgccc cgatgcggtg atcgccaccg ggcgcaccga cttccccaac caggtcaaca
      721 acgtcctcgg cttcccgttc atcttccgcg gtgcgctcga cgtgcgggcg cggaagatca
      781 acgaggagat gaagatggcc gccacgcgcg cgctggcggc gctgaccaag gaggacgtcc
      841 ccgattcggt gctcaaggcc tacggcctgg actcgctccg gttcggcccc gactacctga
      901 tccccaagcc gttcgacccg cgggtgctcc tctgggtggc gccggcggtg gcgtgggcag
      961 cgatcgcgtc gggtgtggcc agggagatca tcgacctcga cgagtaccgc gccaagctcg
     1021 agtcgcggca ggggcgcggg cgggccatca tccgcggcct catcaaccgc gccgtggccg
     1081 agcccaagcg gctggcgttc accgacgccg aggacacccg ggtcatccgc gccgcacgga
     1141 tcgtggccga cgaggggatc ggctcaccgg tcctcctggg cgatgagcgc gaggtcttcg
     1201 aggccgcgac cgaggccggg atcccgctca acaacatcga ggtgctcgac cccgcgcgga
     1261 gcgcgcagcg ggaggcgtac gccacggcct tctggaagcg ccgccagcgc aagggcgtga
     1321 cccggggcga agcacggcgc cggatggccg atcccgcgta ctacggcgcg atgatggtgt
     1381 cgcagggcca cgccgacgcg ctggtggccg gccaggccca gtactacccc gacgccatcc
     1441 ggccggcgct cgaggtggtg ggagtgctcc ccgggggggc ggtggccggc atctacatga
     1501 tgatcgtcgg tgtcaagacg ttcttcttcg ccgactgcac ggtcaacatc gaccccgacg
     1561 cggcgcgcct ggccgagatc gccgaggcgg tggcgggctt cgtgaagcac ttcgggatcg
     1621 accccagggt ggcgctgctc tcgttctcca acttcggctc ggtcaagcac ccggcgcagg
     1681 tcaaggtggc cgaggcggtg cggctcctgc accagcggct gcccggcctc ccggtggacg
     1741 gcgagatgca ggccgacacc gccgtggtcg agtcgatcct gcagcagacg tacgccttca
     1801 gccggttgca gggacctgcc aatgtcctca tttttcctga cctcaactcg gccaacatca
     1861 gctacaagct gctcgcccgg ttgggcggcg cccaggcgat cgggccgatc ctggtgggcc
     1921 tcgccgcgcc gatccacgtg ctgcagcgcg gcgccgacgt ggacgagatc gtcaacatgg
     1981 cggtgatcgc ggcggtggat gcgcaggagc gcagccgccg ggccgcgacc gtcgcccgat
     2041 gacctaccaa cgcacaagga ccccagcatg accgcgacca agcccgcccc cgaggcgatc
     2101 ggcatccccg gcaccggcct ccacccgatc cgggtccacg ccaacctgca tcctccgcag
     2161 ctcatcgagc acgccgtccg ccgcggcgag ggcacgctca ccgcccacgg cgcccacacc
     2221 accatcacct cgccgcacac cggccgttcc cccaacgaca agttcgtggt ggacgaggcg
     2281 agctgcacgg cggagatctg gtgggacaag aacggcaagg gcgacgaggc gggcttcgag
     2341 aagctcctcc aggccgtgcg ggcgcacctc gacgcccagg aggtgttcgt ccaggacctc
     2401 tacgccggcg ccgaccgttc ccaccggctc aacgtccggt tcgtgacacc caacgcctgg
     2461 caggcgctct tcgtgcggaa catgttcatc cggcccgacg cggccgacct ggccgggttc
     2521 gcgccggggt tcaccgtact ccatgcgccg gagctccagg ccgacccggc cgtgcacaag
     2581 acccggaccg gcacgttcgt gatcctccac tttgcccgga agctggtcct catcggcggc
     2641 acccggtacg cgggggagct caagaagtcg atcttcacgg tgctcaatta cctgctgccc
     2701 aggcagggcg tgctgccgat gcactgctcc gccaacatcg gccccgacgg cgataccgcc
     2761 atcttcttcg gcctctccgg caccggcaag acgacgctct ccgccgaccc cgcccggcac
     2821 ctgatcggcg acgacgagca cggctggggc gagcacggcg tcttcaactt cgagggcggg
     2881 tgctacgcca aggtgatccg cctctccgcc gagggcgagc cggagatcta cgccgcgagc
     2941 cagcgcttcg gcaccattct cgagaacgtc gtcgtcgacc ccgccacccg gctcccggac
     3001 tacaacgacc agagcatcac cgagaacacc cgctccagct acccgatcga ctacatcccg
     3061 aaccacgagc cgtcgggcca gggcgggcac cccaggcaca tcgtgttcct gaccgccgac
     3121 gcctacggag tgctgccgcc catcgcgcgg ctctcgccgg aacaggcgat gtaccagttc
     3181 gtctcgggct ataccgccaa ggtggcgggc accgaacgcg gcgtgaccga gccgacggcc
     3241 accttcagcg cctgcttcgg cgcgccgttc ctgccgctcc acccgggggt ctacgccaag
     3301 atgctggggc agaagatcgc caagcacggg gccacctgct ggttggtcaa caccggctgg
     3361 accggcggcg cgtacggcac cgggagccgg atgaagctcg cgcacacgcg gtcgatggtc
     3421 cgcgcgctcc tggccggcaa gctcgccaag gccgagttcc gcaccgaccc ggtgttcggc
     3481 ttcgacgtgc cgaccgcggt gccggacgtg ccggccgcgg tgctcaatcc acgggagacg
     3541 tgggccgaca aggccgccta cgacgcgcag gcgaagaagc tggcccagat gttccgcgag
     3601 aacttcgcca agttcgcgga ccacgtcgag gcgaaggtgc agcgggcggg accgacggtg
     3661 taagtggcgg atgggcggat ggacccgcgg tcgtcgtcgt cggatatgag cggcagcgaa
     3721 aaaggggccc cggggagacc tggggccctt cagcaacgcc tgttcaggag ggcttcaaca
     3781 gctcggttca cccacccttg actctatact atggtataag gtttatactg ctgccatcgg
     3841 acctgagggg ggtgaaatgg cgctcaccat tggtcgtgtc gccgccgagg tcggagttaa
     3901 catccagacg atccgctact acgagcgccg cggactgctt gttcctcccg accgcagtgc
     3961 gtccggctat cgccagtacg ccggcgacgc ggtcagccgg cttcggttca tcaagcatgc
     4021 gcaggagctg ggtttctcct tggcggaagt ccgtggattg ctggatctga gggtccgacc
     4081 cggcgccgcg tgcgatgccg tcgagcagtc gacccgaaag cagatcgatc gggtccaagg
     4141 gaagatccgc agtctccagc gcatcgagcg caccctcgag cgcttggcga ccgcatgcga
     4201 ggcgaggcag gcgacggacg cctgcccgat cttggaggca cttgaagatc atgccaacac
     4261 caacgactaa gacgctcctc ggggccacgg gtggcgtcgc agcggccgtt gcatcggcgc
     4321 tgtgctgtgt cggcccgctc gtcgccgtgg ccctggggct cagtggtgcc gggctcgccg
     4381 caacgtttga accactgcgg ccttacttca tcgccggcac cgtggcagcc cttgggctcg
     4441 gcttcaccac gttgtggcgc gaggagcggc gggcctgcga gcccgggact ccctgcgcct
     4501 cgcccgccgc gcgccgtcgt atgaagcggc tgctctgggt cgcaacgatc attgctgtct
     4561 cgtttctcac cttcccctgg tggtcgaagt accttctcgg ctaggagcct gatatgatcg
     4621 catcaattag gacagcgctc gtactcgtgg cagccgccag cggctccacc ctgctctgtc
     4681 cgcgtggcgg cgaatgcgcc ctgccggcgg catcgaacct ggcacgaatc gccacccagg
     4741 caacggacac cgccaccgtc cagctgcaca tttccggaat gacctgcggg agctgcccga
     4801 tcaccgcgcg catcgctctc aggaagctcc cgggcgtttt cagcgccacc gtgacgctgg
     4861 acgacagtct cggggtcgtg cgctacgacc cgtccaaggt gacccctgcc cagatcgcgg
     4921 cccatctcac tcgactcacc ggctacggcg ccaaggtgat cgccgatggt gccaaggccc
     4981 tccgtcaggg tgggaagtga cggacacgga ccggatgggc atcgggctcc tggtcgcggg
     5041 actggtgtgt tgtgccgcgc ccgtgatact cggcactggc ctggcggccg ccgccctcgg
     5101 cgtcgtgcgt cagcactggg gctggtctgc ggcaggcgtt gggcttgtgg cgctcgtggt
     5161 gatttcatgg gctcggggcc gtcattgcgc cgttcctcgg aaggaactcc aacgatgacg
     5221 gatatgcgcg agctcaccat cgagatccgc ggcatgacgt gcgaccactg tgaacgcgcc
     5281 gtggccacgg cgctgcacgc cgtgcccggg gtccggcggg tcgacgaggt gaaccacgtc
     5341 aggggcttcg cgcgggtcac ggcggggccc gaagccacat ctgccagaat cgaggatgcc
     5401 atcgcgaagg cgggataccg cgcgctcgta cgccccgagc cgacgggcac cgtcgcctcg
     5461 acgacaatcg ggcaacaagc gggcgagttc gacctgacca tcctgggcgg gggctccgcc
     5521 ggattcgccg ctgcaatccg ggcggctgag ctcggcgcac gggtcgccat ggtcgaggcc
     5581 gggaccctcg gcggcacctg tgtgaacgtc ggctgtgtgc cgtcgaagac actgatccgg
     5641 gccgccgaaa tgcagcaccg ccggctgcat cacggattcc aaggcattcc catcactggc
     5701 ggctccgttg actggaactt ggtccgtacc cagaaggacg aactcgtggc cgggcttcga
     5761 caggcaaagt actgggatgt actgcacgcg tacccgtcga tcacactctt cgagcaacag
     5821 gcagagatcg cgtccggcgg cggcgttcgc ctggccgatg gccgtatcat cacgtccggc
     5881 aagatcctcg tcacgacggg agcgtccccg tgggcgccgc cgatccctgg gctggccgac
     5941 gccggccacc tcgacaacgc cagcgcgatg gcattgccgg agttgccgaa ctcgctgatc
     6001 gtgatcgggg cgagcgcggt gggcctcgag cttgcccaga tgttcgcgcg gctgggcgtc
     6061 acggtgacgg tggtcgaggc ccagccacgc gtggtccccg gggaggatcc cgacgtcggt
     6121 gacgccttgg gcgcgtcgct ggagcgggag gggctcgacc tccgtatcgg catgcagatc
     6181 gagcgcgtgg gccgcgacgg gagcgactat accgtgtcgt tcaagggcgg cggcagtgta
     6241 cgcgccgaac agctgctggt ggccaccggc cgccgggcga acgttgcggg cctggggctc
     6301 ggcgccgccg gcgtggcact cggaaagaag ggtgagatcg tcgtcgatca gttccttcaa
     6361 acggccaacc cgggcatcta tgccgccggt gacgtgaccg gtgacccgat gttcgtctat
     6421 gtggccgcgt acggaggaac gatcgcggca gagaacgccc tcagggggaa cgctcgccgg
     6481 tacgatctcg cggccttgcc gaaggtcacg ttcaccgatc cggcggtcgc gtccgtcggg
     6541 ctcaccgccg atcaggcgcg cgcgcagggg atcggccccc tcgtatcact ccttccgatg
     6601 gagcacgtgc cacgctcgct ggcggcccgt gacacgcgcg gcttcatcaa gctcgtggct
     6661 gacgcagcga cccggaagat cgtgggcgcg cacatcctgg cggccgaagc gggcgagatg
     6721 atcaccgagc cggcgatggc catcaaattc gggttgacga tcgacgacct cacgtcgacg
     6781 ttccacccgt acctgacgct atcggaaggg atcaagctcg cagcccagac cttcgaccgg
     6841 gatgtggcga aactctcgtg ttgcgccgcg taggccatcc tggaggaccg ctggtgcgcc
     6901 cgcagctcga ccacaatatg ccaaactccc ccgccgactt cgagatcgtc cgcgacccgc
     6961 tctgggacaa catccgcctc gaccgcgatg tcgccacgct cgtcgacacg cccgcggtcc
     7021 agcgcctgcg ctacgtgcgc cagctgggcc acgccttcct cgtctacccc ggcgccaccc
     7081 acagccgctt cgagcacgca ctcggcacct atcacctggc gcggagcgcc ctctccgccc
     7141 tcgacgaacg gggcgagctc gccggcatcg gcgacgacga gcgcctcgcg gtcgagctcg
     7201 cggccctcct gcacgatatc gggcactatc ccttttccca cgccctcgaa gaggcggggt
     7261 tcctcgacca tgaatccctg ggcgaggcca agctccgcca cggtgagctc ggcgacgcgc
     7321 tccgtgcgct cggcggcgac gcgctcatca cccggatcgg gcggatcatc cggggccgga
     7381 gcgactcgcc cctggcggga ctcatctccg gctccctcga cctcgacaag atcgactacc
     7441 tgagccgcga cgcccggatg tgcggcgtcc cctacggtac ggtcgacgtg agccgcctcc
     7501 tcgccgcgct gacactggtg gagacggggg acggaaggcg ggaggcgggg gtccacgata
     7561 agggagtgag tgccctcgag tcgctgctct ttgccaagta ccagatgtac cggaacgtct
     7621 actggcacca cgccgtccgt gccgccacct gcatgttcaa gcgcgccgtg ggggccgcgg
     7681 tgatcgcggg cgcgctcacg cccgacgcca tcgccgacgc gaccgacgcg agcctgggcg
     7741 aggcgctcct gcggcatgac ccgaccggtc tcgcgcgggc ggtgcacggg cggcgcctcc
     7801 acaagcgggc ggcagacctc gcggccgccg acctccccgc cgacacccag ccgtggatct
     7861 ccgacgaccc ccggctggtc gagcgggtcg aagatgcgct ggcgatcgaa ctgggcctcc
     7921 cggcgggcgg gctcctgctc gatttcccga gccggtccac gatgctgtcg gtggaccttc
     7981 cgctccgcac ccgcactggc gagaccgcgc gcctgaccga cgccggccga gcggggcagt
     8041 tggggctccc gcgggtggcg gaagagctct acc
//
LOCUS       DASUAP010000274         6018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1071510, whole genome shotgun sequence.
ACCESSION   DASUAP010000274 DASUAP010000000
VERSION     DASUAP010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071510"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFU45_07265"
     CDS             complement(<1..473)
                     /locus_tag="VFU45_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5358900.1"
                     /translation="MATKTRTKRRALRPAQIARAVDEGRESLDLYLDEISRVPLLTRD
                     EELELARKAFRGDVKAQEKLARHNVRFVVSVAKKFQNRGVPLVDLIGEGNLGLMTAAR
                     KFDPDRGVKFISYAVWWIRQAVQAAIARHGRPVRVPLNRTADLSRLGRTTTLLKE"
     gene            complement(838..1512)
                     /locus_tag="VFU45_07270"
     CDS             complement(838..1512)
                     /locus_tag="VFU45_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358901.1"
                     /translation="MKHRSTIVLALAAAVATSAAAQGPCGMKGPHLPAAGEWSEYQTA
                     RGTMKMAYLGHESGGDRIEMQMTSDRGNMIMQVVVDGFPYDSKDIHEAVIKLGDRPAM
                     KAPQMMLDRMGGNSAITKDMCGSFTKVGDESVTVPAGTFKATHYKFSSERTINGMDMK
                     SSGDIWISADVPFGLVKEEGTASGMRGDTQTSMALTAKGSGAKSSITEKPQDMGGMMM
                     GPPPKN"
     gene            complement(1533..2921)
                     /locus_tag="VFU45_07275"
     CDS             complement(1533..2921)
                     /locus_tag="VFU45_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU5358902.1"
                     /translation="MTPRELFVQVTYLTASVLFILGLRSLTRPDQARRGMNQAALGML
                     LAICGTLAHHEIVRYDWIIAGLVIGTVIGYPLGVFVPMTAMPQRIAISHLFGALAATL
                     VGIAEYYTLVRGPGVGHAEMAALGFEVLFGSLTITGSFMAFGKLQELLPGRPITYRGQ
                     NAVNLLLFATALGLFGYLIFVPGHQAAFYTMVGIAFCLGIFMVMPIGGADMPVVISLL
                     NSYAGLASSATGFAIGNNVLIIAGALDGASGFLLSILMSKAMNRSFANVLFGAFGSPV
                     AKSGRSSVGLAVRSITAEDAALQLGYAGLVIVVPGYGMAVAQAQHQVRELAEQIEKRG
                     GEVKYAIHPVAGRMPGHMNVLLAEANIPYDRLYDMEEINGQFEQADVALVIGANDVVN
                     PAARSDRSSPIYGMPILNVDRAKHVIVLKRSMNPGFAGIENELFYDPKTSMLFGDAKA
                     SLSRLVTEVKAL"
     gene            complement(2918..3229)
                     /locus_tag="VFU45_07280"
     CDS             complement(2918..3229)
                     /locus_tag="VFU45_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU5358903.1"
                     /translation="MIATGIIQLYVFILAAFVGYQVITRVPPLLHTPLMSATNAISGI
                     SLVGSIVAAGSDRNTVSTILGVIAVASATVNVVGGFLITDRMLKMFKRHDVPGSTPKA
                     G"
     gene            complement(3365..4453)
                     /locus_tag="VFU45_07285"
     CDS             complement(3365..4453)
                     /locus_tag="VFU45_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU5358904.1"
                     /translation="MRIGVPRETTSGERRVALVPESVGRLVKAGIAIVVEQGAGDAAG
                     FPDAAYAAAGATPGSAAAAFGGVDLVLKVQAPTPDEAAQIDRGAIVVAFLAPHTHGVA
                     ISALQARGVTGLAVELVPRTTLAQSMDVLSSQATIAGYKAVLLGAAELPRILPMLTTA
                     AGTLAPARAFVIGAGVAGLQAIATARRLGAVVSGFDVRAAAREQIESLGARSVGKELE
                     TAEGAGGYAREVAADQQARIEQLLAAHLKDQDLVITTAQIPGKPAPRLLTAEMVRTMR
                     PGSVIVDLAAETGGNCVHTRAGETVRAGGVSILGPVNVAASVPSHASQMLSRNLTALA
                     QRMVKDGTLVADAADEVVGPMIVTRPSA"
     gene            complement(4555..>6018)
                     /locus_tag="VFU45_07290"
     CDS             complement(4555..>6018)
                     /locus_tag="VFU45_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358905.1"
                     /translation="ARTIPRAKGDTLSALQSAKAFAAKVSVPLLRLTPVDSIKALVLD
                     PSQLKSLQAAAALQARGDSVRRAVQAGYDSLALQVVLDSSRALADRMAKFDAGAAGVA
                     GTRQAVQDLDQGLSRIKDAQARVSALQRSATAAAASLGDGAKALDAARQQDYALARGL
                     LKLPTVSPRDISAALFGRSVVGKFERAVYWSQMAKRYMPPGLLPKKDPGPRRARMAGT
                     TVRFPELHRLPTFLLRRGDLGISFGGDRASDYALTAALRGVTSDPALYGQPTTFTGAG
                     KAAGPVPVTVDLGGAFDHTGAVASDSAAGTVSGIRLPPFALPGLRYSAAPGVGNASFS
                     FSVRGDQLRGRWTLAAANVSWVRDSAAGPPGTVDQLVGRVLQGIRTLTLEATLSGTLA
                     APQVDVSSNVGDAVAKGVSAMLGQAARDAEAKARAEVDRQVGAKVADAKARVADVQQQ
                     AAKLVADAQSKLEDQRKQLESRLQGLARPSLPGGIKF"
BASE COUNT          931 a         2125 c         2051 g          911 t
ORIGIN      
        1 ttttccttga gcagcgtcgt ggtgcggccc agccggctca ggtcggccgt gcggttgagc
       61 ggcacgcgga ccggacggcc gtggcgggcg atggcagcct gcaccgcctg gcggatccac
      121 cacacggcgt acgagatgaa cttgacgccg cggtcggggt cgaacttccg ggccgcggtc
      181 atgaggccca ggttcccctc gccgatcagg tccaccagcg gcacgccacg gttctggaac
      241 ttcttggcca ccgacaccac gaaccgcacg ttgtggcggg ccagcttctc ctgcgccttc
      301 acgtcacccc ggaacgcctt gcgggcaagc tccagctcct cgtcccgcgt gagcagcggc
      361 actcggctga tctcatccag atagaggtcc agggactccc ggccctcgtc gaccgcgcgg
      421 gcgatctgcg ccggacggag cgcgcgccgc ttggttcgag tcttagtcgc cattgctgtc
      481 tcctcacttc acaacgtccg accgtggcga acgcgccccg ggacggccgt tcaaaaatta
      541 gtcgcgcaat atagctgtta agacactcaa aatcaacagc ttagctaact cgcactcccc
      601 gttggctttg ccaagcacat gggaggacac cccaccgagc cgaccgggcg aaacgccagt
      661 gtgtcatttc aacatcggac atggccagca acggccctcc cggccttaaa gacggcgcaa
      721 tgaccccgcc gttgcaccgc caccctagag acgcgcaaac cggccggaac gttcccccga
      781 ataccaacga cccccgggga tacgctcccc gggggtcggc ggatcggtgg gacgcgatca
      841 gttcttcggc ggcggcccca tcatcatgcc gcccatgtcc tgcggcttct cggtgatgga
      901 gctcttggcc ccggagccct tggcggtgag cgccatggat gtctgggtat cgccccgcat
      961 cccggacgcg gtgccctctt ccttgaccag gccgaacggg acgtccgcgc tgatccagat
     1021 gtcgccggag ctcttcatgt ccatcccgtt gatggtccgc tcagagctga acttgtaatg
     1081 agtggccttg aaggtcccgg ccggcacggt gacagactca tcgccgacct tcgtgaagct
     1141 gccgcacatg tccttcgtga tggcgctgtt gcctcccatc cggtccagca tcatctgggg
     1201 cgccttcatc gccggccggt cgccgagctt gatcacggcc tcgtggatgt ccttcgagtc
     1261 atacggaaac ccgtccacga ccacctgcat gatcatgttg ccgcggtcgc tggtcatctg
     1321 catctcgata cggtcaccgc cggattcgtg gcccaggtag gccatcttca tggtcccgcg
     1381 ggccgtctgg tactccgacc attcccccgc cgccggaagg tgggggccct tcatgccgca
     1441 cggcccctgc gcggcggccg aggtcgccac cgcggcggcc agggccagga cgatcgtgct
     1501 acgatgcttc atacgttctc cactgaaagg gttcagagtg ccttgacctc ggtgaccagc
     1561 cgcgacagcg aggccttcgc gtcgccgaag agcatcgacg tcttcgggtc gtaaaagagc
     1621 tcgttctcga tcccggcgaa gcccgggttc atcgagcgct tcaggacgat aacgtgcttg
     1681 gcccggtcca cgttgaggat cggcatgccg tagatggggc tcgaccggtc gctccgggcc
     1741 gcggggttca ccacgtcgtt ggcgccgatc accagcgcca cgtccgcctg ctcgaactgc
     1801 ccattgatct cctccatgtc gtacagccgg tcgtagggga tgttggcctc ggccaggagc
     1861 acgttcatgt gccccggcat ccggcccgcc accggatgga tggcgtactt gacctcccca
     1921 ccccgcttct cgatctgctc ggccagctca cgcacctggt gctgggcctg ggccacggcc
     1981 atcccgtacc cgggcacgac gatgacgagc cccgcatagc cgagctgcaa cgccgcgtcc
     2041 tcggccgtga tcgagcgcac ggcgagcccc accgacgagc ggccgctctt ggccaccggc
     2101 gagccgaacg ccccgaagag cacgttggcg aagctccggt tcatcgcctt cgacatcagg
     2161 atggagagca ggaatccgct ggccccgtcg agcgcaccgg cgatgatgag gacgttgttg
     2221 ccgatcgcga acccggtggc cgacgatgcg aggccggcgt acgagttcag gagcgagatc
     2281 accaccggca tgtcggcgcc gccgatcggc atcaccatga agatgccgag gcagaaggcg
     2341 atccccacca tcgtgtagaa ggccgcctgg tggccgggca cgaagatgag gtagccgaag
     2401 agccccagtg ccgtcgcgaa gaggagcaga ttgaccgcgt tctggccccg gtaggtgatg
     2461 ggccggcccg gaaggagctc ctggagcttc ccgaacgcca tgaaactgcc cgtgatggtc
     2521 aggctgccga agaggacctc gaacccgagc gccgccatct cggcgtggcc caccccgggg
     2581 ccgcggacca gggtgtagta ctcggcgatg cccaccagcg tggcggcgag cgccccgaac
     2641 aggtgggaga tggcgatccg ctgcggcatc gcggtcatcg gcacgaacac gccgagggga
     2701 tagccgatga cggtcccgat cacgagcccg gcgatgatcc agtcatatcg gacgatctcg
     2761 tggtgcgcga gcgtgccgca gatggccagc agcatgccga gtgccgcctg gttcatcccg
     2821 cgacgggcct ggtccggccg cgtgagcgac cggagcccca ggatgaacag gaccgaggcg
     2881 gtgaggtagg tgacctgtac gaacagttcg cgcggggtca tccggccttc ggcgtggagc
     2941 cgggaacgtc gtgccgcttg aacatcttga gcatccggtc ggtgatcagg aacccgccca
     3001 cgacgttgac cgtggccgac gcgacggcga tgacgccgag gatggtgctc accgtgttgc
     3061 ggtcggagcc ggcggcgacg atcgacccga ccagcgagat gcccgagatc gcgttggtgg
     3121 cgctcatcag cggggtgtgc aggagcggcg gcacccgggt gatgacctgg tagccgacga
     3181 aggccgccag gatgaagaca tagagctgga tgatgccggt ggcgatcatg ggtcgagggc
     3241 tccgggacgg cgtagggcta tcagctatcg gctatcagct atcagctatc agctatcagc
     3301 tatcagcgcg aaggttgcac ggcacgcgca gtgcgcaaga cccgcccggc cagttcacgg
     3361 cgcttcaggc gctgggtctc gttacgatca tcggaccgac gacctcgtcc gcggcgtccg
     3421 cgacgagcgt gccgtccttg accatccgct gggcgagagc ggtgaggttc cggctcagca
     3481 tctgactggc gtggctcggt accgacgccg cgacgttgac gggcccgagg atgctgaccc
     3541 cgcccgcccg gacggtctcc ccggcccgcg tgtggacgca attcccgccg gtctccgcgg
     3601 ccaggtcgac gatgacggaa cccggacgca tcgtccgcac catctccgcg gtgaggagcc
     3661 tcggcgccgg cttccccggg atctgggcgg tggtgatcac caggtcctgg tccttgaggt
     3721 gcgcggcgag gagctgctcg atccgggcct gctggtcggc cgcgacttcg cgggcatagc
     3781 cgccggcacc ctcggcggtc tcgagctcct tcccgaccga ccgggcgccc aggctctcga
     3841 tctgctcccg ggccgccgcg cggacgtcga agccggagac gacggcgccg agccggcggg
     3901 cagtcgcgat ggcctgcagc cccgccacgc cggctccgat cacgaatgcc cgcgccggcg
     3961 cgagcgtccc ggcggcggta gtgagcatcg gaaggatgcg gggaagctcc gccgcgccga
     4021 ggagcaccgc cttgtatccg gcgatcgtcg cctgagagga aagcacgtcc atcgactgcg
     4081 ccagcgtggt ccgcggcacc agctccaccg ccagcccggt cacgccccgc gcctggagcg
     4141 ccgagatcgc gacgccgtgg gtgtgggggg caagaaatgc gacgacgatg gcgccccggt
     4201 cgatctgggc cgcctcgtcc ggggtgggcg cctgcacctt gagcaccagg tccacgccgc
     4261 cgaacgccgc cgctgccgat cccggcgtcg ccccggcggc ggcataggcc gcgtcgggga
     4321 agccggcggc gtcccccgcc ccctgctcga cgacgatggc gatccccgcc ttgacgagcc
     4381 gcccgaccga ctcggggacc agcgccaccc gacgttcccc ggacgtcgtt tcccgcggca
     4441 caccgatgcg catcgccgat cccggttacg gtggagtgac gcgcaagaat ctacgcccac
     4501 tgcgggcggg cgggaggggt cgccggccgc gcggccggcg accggatgcc ggggctagaa
     4561 cttgatcccg cccgggagcg atggacgggc gaggccctgc aggcgggact ccagttgctt
     4621 gcgctggtcc tcgagcttcg attgcgcgtc ggccacgagc ttggcggcct gctgctgcac
     4681 gtcggccacg cgggccttcg catcggccac cttggcgccg acctgccggt cgacctcggc
     4741 ccgggccttg gcttcggcgt cacgcgcagc ctggccgagc atcgccgaga cccccttggc
     4801 cacggcatcg cccacgttcg agctgacatc cacctgcggc gccgcgagcg tcccgctcag
     4861 cgtcgcctcg agcgtcagtg tgcggatccc ctgcagcacc cgcccgacga gctggtccac
     4921 cgtaccgggg ggaccggcgg cgctgtctcg gacccaggaa acgttcgccg ccgcgagggt
     4981 ccaccggccg cggagctggt cgccgcggac cgagaaggag aagctggcgt tgccgacacc
     5041 cggcgcggcg gagtaccgga gcccggggag cgcgaacggc ggcaaccgga tgccgctcac
     5101 agtgccggcc gcactgtcac tggccacggc accggtgtga tcgaacgcgc cgcccagatc
     5161 gaccgtgacc ggcaccggcc cggccgcctt ccccgccccg gtgaaggtgg tcggctggcc
     5221 gtagagcgcc gggtcggatg tgacgccccg cagcgccgcc gtcagggcat aatcgctcgc
     5281 ccggtcaccg ccgaaggaga tgccgaggtc gccgcggcgc agcaggaacg tcggcagccg
     5341 gtgcagttcg ggaaagcgga cagtggtgcc ggccatccgc gcccgccgcg gtcccgggtc
     5401 cttcttgggc aggagcccgg gcggcatata gcgcttggcc atctgactcc agtagaccgc
     5461 acgctcgaac ttgcccacca ccgaccgccc gaagagcgct gcactgatgt ccctggggga
     5521 caccgtcggg agcttgagga gaccccgtgc cagggcatag tcctgctgcc gggcggcatc
     5581 gagcgccttg gcaccgtcgc cgagcgacgc agcggcggcc gtcgccgacc gttgcagcgc
     5641 cgacacccgc gcttgggcgt ccttgatccg ggacagcccc tggtcgaggt cctgcaccgc
     5701 ctggcgcgtc ccggcgacgc cggccgcgcc ggcatcgaac ttcgccatcc ggtcggccag
     5761 cgcccggctg gaatccagca ccacctgtag cgccagggag tcgtagcccg cctgcacggc
     5821 cctgcgcacc gaatcgccgc gggcctggag tgcggcggcc gcctgcagcg acttgagctg
     5881 cgagggatcg agcaccagcg ccttgatgct gtcgaccggc gtgagccgca ggagcggcac
     5941 gctgaccttg gccgcgaacg ccttggcgga ctggagggcg ctcagggtgt cgcctttggc
     6001 cctggggatc gtccgggc
//
LOCUS       DASUAP010000275         6824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1044138, whole genome shotgun sequence.
ACCESSION   DASUAP010000275 DASUAP010000000
VERSION     DASUAP010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044138"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2647
                     /gene="hrpB"
                     /locus_tag="VFU45_07295"
     CDS             <1..2647
                     /gene="hrpB"
                     /locus_tag="VFU45_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEU5358906.1"
                     /translation="VARNSAVLQAPPGAGKTTLVPLALLNEPWLAGKKIVMLEPRRLA
                     ARAAATRMTELVASGAFPLSADVERGSGGEETRAGRSFASPRTTKEMKPDGEAARDPR
                     LATIGGLIGYRIRRDTKVSSATRIEVVTEGVLTRIIQHDPALEDYGLVIFDEFHERNL
                     NGDLGLALTLQTQSLLRPDLRILVMSATLDGGPVSALLGDAPIVTSEGMSFPVETRWI
                     PRRPDQRVEAAMASAIRQALDQHDGDILAFLPGAREIHRTRELLAESPLTADRSPLTD
                     TIPLFGMLPQAEQDRAIRPSAPGRRKVVLATSIAETSLTIEGVTVVVDSGLARVPRFT
                     PASGMSRLATIRVSRASADQRRGRAGRTSPGTCYRLWAQAEEPGLIPRATPEILEADL
                     APLALDLAAQGIADPLTLRWLDLPPGAAFAQARELLRELGALDASGAISPHGHAMAAL
                     PVHPRLAHMVIRSQDAGLGELGVQLAALLEERDILRGSGGPPDPDIELRLHALRDRDA
                     APNVDRDALHRVRAEAKALAEIIGRTPSPRTGRGGRGERKKDGTLEAGGGSPQATPAA
                     LLALAYPERVGRRREGQRGRFTLRNGAGVWTDSPSFVNAEWIVVAETDGDPRDAKIYL
                     GAALDEAEVMDLFADQVMEEERVAWDPAIGAVLAQRDRRLGAIVLGSAPIRDAEPSHI
                     TAAMTAAIRREGLSLLPWNDVATSTRQRLAFLHRLDPAWPDVSDQALLERLDGWLGPA
                     LGAIRRKGDLARLDLDAALRTTLDWKRLQALDELAPTHVSVPSGSRIAVDYGDPAAPV
                     LAVKLQEVFGWTDTPRIGGGKVPLTLYLLSPARRPIQVTRDLASFWRNAYFEVRKDLR
                     GRYPKHPWPEDPLSATPTRRTKRAR"
     gene            2829..3479
                     /locus_tag="VFU45_07300"
     CDS             2829..3479
                     /locus_tag="VFU45_07300"
                     /inference="COORDINATES: protein motif:HMM:NF015866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU5358907.1"
                     /translation="MSESTVLFEACVESVAEAVAAERGGAGRIELCVDLAIDGITPPM
                     EMITEACRAVKVPVMVLVRPRGGEFRYSPIEVSAMIGSIERARSLGAAGVVIGALAAD
                     GGIDRAVTERLVKAARPMSVTFHRAFDVVPDRADALETLVTLGVDRVLTSGGAPTAEA
                     GVLELKRLVDQAAGRIGILAGGRIRAGNVAAIVRATGVREVHAHTDAAELMKALRR"
     gene            3636..4766
                     /locus_tag="VFU45_07305"
     CDS             3636..4766
                     /locus_tag="VFU45_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358908.1"
                     /translation="MRVRLRALLAIALVSGCGGSTGPTKAAPSAPVRQLSSGGDVSCA
                     VTADSTAWCWGRGFGPKPVVWASGTKFRRVSVALSGASVSYVCGVAVSGQTLCQGTAT
                     VDSSGSWVLGASPTPLVNDTVIDTVSAGGSHFCGVNAAHAIWCWGQYAAGVRGDSIVP
                     AARDSFDVPSKVAGGISWAQVAAGTDHTCGLAGNGAVYCWGKATQVGVGDTTQYAAFD
                     TTCNVSAGGSGRCAYAPLAVAGVPSSIRLVSSGTTTCAITSSYTLWCWGYVGPTAGNA
                     GQVPAAMPISTVATTVTVGSLGGYCIISPAGDIYCGTLGSAPSILQGGLKFTDVSAGS
                     DHACGIGAGGYVYCWGQNGSGQLGVGDSTARSLPTQVVIPIP"
     gene            complement(4750..4959)
                     /locus_tag="VFU45_07310"
     CDS             complement(4750..4959)
                     /locus_tag="VFU45_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HEU5358909.1"
                     /translation="MTWRRRITGFHRDDEGHWVAELECGHGQHVRHDPPWQVREWVTT
                     AEGRAGRIGTELECRRCEVAARGSG"
     gene            complement(4956..5129)
                     /locus_tag="VFU45_07315"
     CDS             complement(4956..5129)
                     /locus_tag="VFU45_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358910.1"
                     /translation="MKTLGWLGGLVGSTAAGTFGWWLGGFVGSTTAFIISGIMGGVGL
                     YYGRKWALQMVGM"
     gene            complement(5323..6678)
                     /locus_tag="VFU45_07320"
     CDS             complement(5323..6678)
                     /locus_tag="VFU45_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5358911.1"
                     /translation="MTAPVTYRRSLGLFSGTMLVIGGIIGSGIFLNPAIVAQRVGTAG
                     LTMLAWGLGAVVALIGAFIFAELGQRRPRAGGGYIYLRDAFGPVYAFLYPWTLFLMTA
                     TGAIAAVAVTFSTYAGPLLGLPPALEKPLAAAAIALLTGVNVIGIRPGAWTQNVFTVL
                     KLAAIAVLVFAGLALSPIHPHVCGTCTAAAPPPATLGAAILAMGAALVPVLFAFGGWQ
                     QTNFVAEELRDPERNLPRALVLGVIAVVVVYLTVNLAYLRALGADGLAASTAPAAETM
                     EIYLGAAGRTLIAAGIVISTFGFLNLVILVTPRVYQATAADGLFFAGFSRLHPTFRTP
                     VIALVAQGIWGIILLYSGTYGQLLDYVVFADWIFFGSNAVALFVLRRQDIAAGRDTAV
                     TVRTPLHPWSTAVFILASVYVLVGTITSNPANALIGAGFLAIGVPVCLFWRHRARLEA
                     R"
BASE COUNT         1021 a         2414 c         2400 g          989 t
ORIGIN      
        1 cgtcgcgcgg aactcggcgg tgctccaggc gccgcccggc gcggggaaga cgacgctggt
       61 tccccttgcc cttctgaacg agccgtggct cgccgggaag aagatcgtga tgctcgagcc
      121 gcggaggctc gcggcgcgcg cagctgcgac ccggatgacc gagctggtgg ccagtggggc
      181 gttccccctc tccgcggacg tggagagagg gtcggggggt gaggagacgc gagcgggccg
      241 atcttttgct tcgcccagga cgacgaaaga gatgaagccg gatggtgagg cagcgcgcga
      301 cccgcgactc gcgaccatcg gcggcctcat cggctaccgc atccgccgcg acaccaaggt
      361 gagctcagcc acgcgcatcg aggtcgtcac cgaaggggtc ctcacccgca tcatccagca
      421 cgatccggcg ctcgaggatt acggcctcgt gatcttcgac gagttccatg agcggaacct
      481 gaacggcgac ctcgggctcg ccctcacgct gcagacgcag tcgctgctcc gccccgacct
      541 ccgcatcctc gtgatgtcgg ccaccctcga cggcggcccc gtctccgccc tcctcggcga
      601 cgcgccgatc gtgacgagcg aggggatgag ctttccggtc gagacccgct ggatcccccg
      661 tcgcccggat cagcgcgtcg aagccgcgat ggcctcggcg atccgccaag ccctcgacca
      721 gcacgacggc gacatcctcg ccttccttcc gggtgccagg gagatccatc ggacccggga
      781 gcttctcgcc gagagcccgc tcaccgctga ccgctcaccg ctcaccgata ccatccccct
      841 cttcgggatg ctcccgcagg ccgagcagga ccgcgctatc cggccgagcg cgccgggacg
      901 ccggaaagtg gtgctcgcga cctcgatcgc cgagacgagc cttaccatag aaggggtgac
      961 ggtcgtggtc gattcggggc tcgcccgggt gccccgtttc acgcccgcct cggggatgtc
     1021 ccgcctggcc accatccgcg tttcccgcgc ctccgccgac cagcgccgcg gccgggccgg
     1081 ccgcacgtcg cccggcacct gctaccggct ctgggcccag gccgaggaac ccgggctcat
     1141 cccgcgggcg acaccagaga tcctcgaagc cgacctggcg ccactggcgc tcgacctcgc
     1201 ggcccagggc attgccgacc cgctcaccct ccgctggctc gaccttcctc ccggcgccgc
     1261 ttttgcccag gcgcgggagc tcctccgcga actcggcgcc ctcgacgcca gtggggccat
     1321 ctcgccgcac ggacacgcga tggcggcgct cccggtccat ccgcggctgg cgcatatggt
     1381 catccggagc caggacgcgg ggctcggcga gctcggtgtc cagctggctg cactcctcga
     1441 ggagcgggac atcctgcgcg gcagcggcgg gccccccgac cccgacatcg aactccgcct
     1501 ccacgccctt cgcgatcgtg atgccgcgcc gaacgtcgac cgcgacgcgc tccaccgcgt
     1561 ccgcgccgag gcgaaggcgc tggctgagat catcgggcgc accccctctc cgcggacggg
     1621 gagaggggga cggggggaga ggaagaagga cgggacgttg gaggccggcg gcgggtcgcc
     1681 gcaggcgacc cccgccgcgc tcctcgccct cgcgtatccc gagcgcgtcg gccggcgccg
     1741 ggagggccag cggggccgct tcacgctccg caacggcgca ggcgtgtgga ccgattcgcc
     1801 gtccttcgtg aacgccgaat ggatcgtggt ggccgagacc gacggcgacc cccgcgacgc
     1861 aaagatctat ctcggcgccg cactcgacga ggcggaggtg atggaccttt tcgccgatca
     1921 ggtgatggaa gaggagcgtg tcgcctggga tccggcgatt ggcgccgtct tggcgcagcg
     1981 ggatcgccgc ctgggcgcca tcgtcctggg cagcgctccg atccgggatg ccgagccttc
     2041 gcatattacc gcggcgatga ccgccgccat ccgccgcgaa gggctgtcgc tcctgccgtg
     2101 gaacgacgtg gcgacgagca cccgccagcg gctcgccttc ctgcaccggc tcgaccccgc
     2161 ctggccggat gtgtcggatc aggcgctgct cgagcggctc gacggctggc tcggtcccgc
     2221 gctcggcgcc attcgccgca agggcgatct ggcccggctc gacctggacg cggcgctgcg
     2281 cacgacgctc gactggaagc gcctgcaggc gcttgacgag ctggcgccga cgcacgtgag
     2341 cgtgcccagc ggctcccgga tcgcggtgga ttacggcgac ccggcggcgc cggtgcttgc
     2401 ggtcaagctg caggaggtct tcgggtggac cgacacgccg aggatcgggg gcggtaaggt
     2461 gccgctgacg ctgtatctcc tctcgccggc gcgccggccg atccaggtga cccgcgacct
     2521 cgcgagcttc tggcgcaacg catatttcga ggtgcggaag gacctgcgcg gccggtaccc
     2581 gaagcacccg tggcccgagg atccgctttc cgccactccg acccggcgga cgaagagggc
     2641 cagatgactg atgatatggg accacggagg gcacggagga tcacggagag gcacggagga
     2701 aatgcttctt tcgtgggtgt gcgagcggaa ctccgatcgc aaggccactc cacctgcagt
     2761 cctctgtgta tctccgtgca gctccgtgcc ctccgtggtc ccatgccgtt cagcatgctg
     2821 gagcttcgat gagtgaatcg actgtactct ttgaagcctg cgtcgagagc gtcgcggaag
     2881 cggtggcggc ggagcggggg ggcgcggggc ggatcgagct ctgcgtcgac cttgccatcg
     2941 acggcatcac gccaccgatg gagatgatca ccgaggcgtg ccgagcggtg aaggttcctg
     3001 tgatggtgct cgtccggccc aggggcggcg aatttcgcta ttcgccgatc gaggtatcgg
     3061 cgatgatcgg atccatcgaa cgggcccgct ccctcggcgc ggcgggtgta gtgatcggtg
     3121 cgctggccgc cgatggtggc atcgaccgcg cggtgacgga gcgactcgtg aaggcagcac
     3181 gaccgatgtc cgtcaccttt caccgcgcgt tcgacgtggt gcccgaccgc gccgacgcgc
     3241 tcgagacgct cgttacgctc ggcgtcgacc gggtgctcac ctccggtggt gcgccgaccg
     3301 cggaggcggg tgttctggag ctgaagcggc tcgtggacca ggccgcaggc cggatcggca
     3361 tcctggccgg cggaaggatc cgcgccggga acgtggcggc catcgtgcgc gccaccggcg
     3421 tccgcgaagt ccacgcccac accgacgccg cggaattgat gaaggccctg cgccggtagc
     3481 agcccccggt ccccggcttt cctacgcttt cccccgcctt cctacgcttt ccccgctttc
     3541 ctacgccttc ccccgctttc ctgtgcgccc gctccccgct gagccgtccc cgcgactata
     3601 atccaagccc atttcctgtc accgtaggag atcccgtgcg agtccgactt cgcgcgctct
     3661 tggccattgc cctcgtgtcc ggatgcggcg gctcgaccgg cccgacgaag gccgccccct
     3721 ccgcgcccgt ccgccagctc tcctcggggg gcgacgtgag ctgtgccgtc accgccgaca
     3781 gcaccgcctg gtgctggggg cgcggcttcg gtcccaagcc ggtggtctgg gcgtccggca
     3841 ccaagttccg ccgggtgtcg gtggcactct cgggcgcgtc cgtgagctac gtgtgcggcg
     3901 tcgcagtgag cggccagacc ctctgccagg ggacggccac ggtcgactcg tccgggtcgt
     3961 gggtgctcgg cgcctctccc acgccgctcg tcaacgacac tgtcatcgac acggtgagtg
     4021 ccggcggatc gcacttctgc ggcgtgaatg cggcgcacgc catctggtgc tgggggcagt
     4081 acgccgcggg cgtgcggggc gacagcatcg tgcccgcagc gcgcgacagc tttgacgtgc
     4141 cgagcaaggt ggcgggcggg atcagctggg cgcaggtcgc cgccgggacc gaccacacgt
     4201 gcgggcttgc aggcaatggg gccgtctatt gctgggggaa ggcgacgcag gtcggcgtcg
     4261 gcgataccac gcagtacgcc gcgttcgaca ccacctgcaa tgtgagcgcg gggggctcgg
     4321 ggcggtgcgc gtacgcgccg ctggcggtgg caggcgtgcc gtcctcgatc aggctggtga
     4381 gcagcggcac caccacctgc gccatcacgt cgagctacac gctctggtgc tggggatacg
     4441 tcgggcccac ggcggggaat gccggtcagg tcccggccgc gatgccgatc agcaccgtgg
     4501 ccacgacggt gacggtaggg agtctcgggg gatactgcat aatttctccc gccggggaca
     4561 tctactgcgg gacgctggga tcggcgccat cgatcctgca gggcgggctc aagttcaccg
     4621 atgtctccgc cggctcggac cacgcctgcg gcatcggcgc cggaggctac gtctactgct
     4681 ggggccagaa cggcagcggc cagctcggcg tcggcgacag caccgcccgg agcctcccga
     4741 cccaggtggt catcccgatc ccctagccgc gacttcgcag cgccggcact ccagttcggt
     4801 gccgatccgg cccgcccggc cctccgcagt ggtcacccac tcgcggacct gccacggcgg
     4861 gtcgtgtcgc acgtgctggc cgtgcccgca ctcgagctcg gccacccagt gcccttcgtc
     4921 gtcccgatgg aagcccgtga tccggcgccg ccaggtcaca tcccgaccat ctgcagcgcc
     4981 cacttcctgc cgtagtagag ccccacgccg cccatgatgc ccgagatgat gaacgcggtg
     5041 gtggacccga cgaacccgcc gagccaccag ccgaaggtgc cggccgcggt gctgccgacc
     5101 aaccctccca gccagccgag cgtcttcatg cgtgcgcctc cgaaagtcgt gtggatcgcg
     5161 gatggtggga ccacggagga cacggagctg cacggagagg ctcggaggaa cggcacatgt
     5221 ggtggtcttg cgggcgggac cccgctcgcg aggccacacc ccatgcagtt ctccgtgtgt
     5281 ctccgtgacc tccgtgccct ccgtggtccc accttcgtgg ccttaccgag cttcgagccg
     5341 cgcgcggtgc cgccagaaca ggcacaccgg caccccgatg gccaggaacc ccgcgccgat
     5401 gagcgcgttg gccgggttcg acgtgatggt gcccaccagc acgtacaccg aggccaggat
     5461 gaacactgcg gtgctccacg gatggagcgg cgtgcggacc gtcaccgccg tgtcgcggcc
     5521 tgcggcgatg tcctgccggc gcagcacgaa gagcgccacg gcgttgctgc cgaagaatat
     5581 ccagtcggca aagacgacgt aatccagcag ctgtccgtag gtgccggagt acaggaggat
     5641 gatgccccag atcccctgcg ccacgagcgc gatcacgggg gtgcggaatg tggggtgcag
     5701 gcgcgagaat cccgcaaaga agagcccgtc ggccgccgtg gcctggtaca cccggggcgt
     5761 caccaggatg accagattga ggaacccgaa cgtcgagatc acgatgccgg ccgcgatcag
     5821 cgtgcggccc gccgcgccga ggtagatctc catcgtctcg gcggcgggtg cggtgctggc
     5881 cgcgagcccg tcggcgccga gggcccggag gtacgcaagg ttgaccgtga ggtacaccac
     5941 caccaccgcg atcacgccga ggacgagggc gcgcggcagg ttcctctcgg ggtcgcgcag
     6001 ctcctcggcc acgaagttgg tctgctgcca gccgccgaag gcgaagagca ccggcaccag
     6061 cgccgcgccc atcgccagga tggcggcgcc gagcgtcgcg gggggaggcg ccgcggcggt
     6121 gcaggtgccg cagacgtggg ggtggatcgg cgagagcgcg agccccgcaa agaccagcac
     6181 cgcgatcgcc gcgagcttga ggaccgtgaa gacgttctgg gtccaggccc cggggcggat
     6241 gccgatgacg ttgacgccgg tgaggagcgc gatcgcggcg gccgcgagcg gcttttccag
     6301 cgccggcggc agcccgagga gggggcccgc gtaggtcgag aaggtcaccg ccaccgcggc
     6361 gatcgccccg gtggccgtca tcaggaagag cgtccacgga tagaggaagg cgtacaccgg
     6421 tccgaaggcg tcgcggaggt agatgtatcc gcccccggcg cgcggccgcc gctggcccag
     6481 ctccgcgaag atgaacgcgc cgatgagcgc caccaccgcg ccgagccccc acgcgagcat
     6541 cgtgagtccc gccgtgccga cccgctgggc cacgatcgcc gggttgagga agatccccga
     6601 gccgatgatc ccgccgatca cgagcatcgt gccagagaag agacccagcg agcggcgata
     6661 cgtcacagga gcggtcatcg gggatctcgg tattggtgac ttggacgtgg gaccacggag
     6721 gacacggagc tgcacggaga ctcacggaga actgcaattg acgtggtctc gcggagggag
     6781 ccccccctgg gaggccacat catgtggctg tcctccgtgc ctct
//
LOCUS       DASUAP010000276         4564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_948746, whole genome shotgun sequence.
ACCESSION   DASUAP010000276 DASUAP010000000
VERSION     DASUAP010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948746"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1426)
                     /locus_tag="VFU45_07325"
     CDS             complement(32..1426)
                     /locus_tag="VFU45_07325"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5358912.1"
                     /translation="MSQKPSVLIVDDESGILDTLRILLRNEGFEVTTAQGGKAGLEQI
                     RSGHHEIILTDVRMPGVSGLDILAAAKEQDTMTPVILMTAQADLRSAIDALNTGAYYY
                     IQKPFGNDDLVAIMRRASEYRAVRVENKQLRQEIRRRDRGVSVERPVGKSRRFLEVLK
                     LAEHVAPTDSTVLITGESGTGKEVVARYIHNLSSRAEGPFMSINCGALPENLLESELF
                     GHVKGSFTGAVRDKQGLFAAARGGTFFLDEVGEMPASLQVKLLRVLQEREVIPVGATE
                     SIPVDVRIVAATNKNLEEEIRRGNFRSDLFYRLNVIALDLPPLRERRDDLLLLIEALL
                     TRLAAENGGEIKALSSEALDAVVVYDWPGNVRELENALEHAVVLSRGTVIDASSLPER
                     ITTRRKEPLVAERSYKNPALDVIERAYIMWVLQAEGGNKTRAAEVLGIDPSTLYRKLS
                     RYEEQPEGKPVTAG"
     gene            complement(1423..3084)
                     /locus_tag="VFU45_07330"
     CDS             complement(1423..3084)
                     /locus_tag="VFU45_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5358913.1"
                     /translation="MNDADRAAARSRLPDRRRLLTWLFAGRLALAVAVLVAAGLAWTE
                     APQVSFLASVGIILALVVTAYGVWVVVSARREPGPVFLAVQLLADLGLVLTLVHFLGP
                     AGGTGPALFVLLIAAYAVLLPFRFGTLTTLLASTVYLVTAYRGGVAPVALALQVVVFF
                     LVFAVVAVLGQRLREASVEHRTLEYELQRVRLEADVILRNIHSGVLSVDSGGNLAYAN
                     PTAQRLLGLERGPAIGAPVMNIVRSRAPGLWSALVEGLEQGRRVRRGEAAVDNGGGRQ
                     FSIGLSTTTFQQPGQSGPSVTAVFSDISDSKRVEELQLRAERLEAVTALSASLAHEIK
                     NPLASIRSSVEQLARSSYAGEDEQTLARLIVRESDRLSRLLTEFLDFSRVRVTKSEPV
                     DLREVADAVVRLVSAHPDHKAATRLGVEGESTVVQGDEDLLHRTLANLVLNAVQVAGP
                     EPVEVTVALKTLAGNELPPGMPRISRGVRLQVKDTGPGIPEDIRARLFEPFVSRRPGG
                     SGLGLAIVQRAVEAHRGTIFVESAAGAGTAFTIYLPAGDAGEESA"
     gene            complement(3081..3356)
                     /locus_tag="VFU45_07335"
     CDS             complement(3081..3356)
                     /locus_tag="VFU45_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358914.1"
                     /translation="MRARMFLAGAAVLLLAACEPDYGKEQSHPATVESEDAVTGGADP
                     TAMSGRVKKPGEANIQHPRADGNAGRPPDGPGAPASSADSTHASGGA"
     gene            complement(3529..>4564)
                     /locus_tag="VFU45_07340"
     CDS             complement(3529..>4564)
                     /locus_tag="VFU45_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5358915.1"
                     /translation="GVAHDFNNLLMVIMGFADFLSGSLEEDDPRRSDAVEIRKASERA
                     AALTQQLMVFGRRGPVAVTAVSLNAAVDELAAMLRAVLGENIALVLELAPDAGGVSVD
                     RGQLDQALLNLALNAKDAMPEGGRLTLRTRRPATGGLALVEVEDTGHGMDDETQARIF
                     EPFFSTRRSSHNTGLGLSVVYGIVAQHRGQVRVRSAPGRGSVFTLAFPRTRLTPPPAV
                     IPEVPAGKVSGSVLVVEDDPDVRQLVSRALGEAGFDVVSAADADEAITMLRREGPVGV
                     LVTDLVLPGMTGAELAARARHEQADLAVVFMSGYGDEDKERPDSRPVLAKPFAPGELV
                     RRVRDAMGRM"
BASE COUNT          684 a         1680 c         1514 g          686 t
ORIGIN      
        1 caccatcgat cgaacgcgga cccggcccgc atcaccccgc cgtcaccggc ttcccctccg
       61 gctgctcctc gtagcggctg agcttccggt acagggtcga gggatcgatc cccagcacct
      121 cggcggcgcg ggtcttgttc ccgccctccg cctgcaagac ccacatgatg tacgcccgct
      181 cgatgacatc cagcgccgga ttcttgtagg accgctccgc caccagcggt tccttccggc
      241 gggtggtgat ccgctccggc agcgaagagg cgtctatcac cgtgccgcgc gacagcacca
      301 ccgcgtgctc cagggcgttc tccagttccc gcacattgcc gggccagtcg tagaccacta
      361 ccgcatccag cgcctccgag ctcagcgcct tgatctcgcc cccgttctcg gcggcgagcc
      421 gcgtgagcag cgcctcgatg agcaggagaa gatcgtcccg ccgctcccgg agcggcggca
      481 ggtcgagcgc gatgacgttg agtcggtaga acaggtccga gcggaagttc ccccggcgga
      541 tctcttcctc gaggttcttg ttcgtggccg ccacgatccg gacatccacc gggatggact
      601 cggtggcgcc caccgggatc acctcccgct cctgcaggac ccggagcagt ttcacctgca
      661 gcgaggcggg catctccccc acctcgtcca ggaagaatgt cccgccccgt gccgccgcga
      721 agagcccctg cttgtcccgc accgcgccgg tgaagctccc cttcacatgc ccgaacagct
      781 cgctctccag caggttctcg ggcagcgcgc cgcagttgat ggacatgaac gggccttcgg
      841 cccgcgacga gagattatgg atgtagcgcg ccaccacttc cttgccggtg ccgctttccc
      901 cggtgatgag cacggtggaa tcggtcggtg cgacatgctc cgccagcttc agcacctcca
      961 ggaaccggcg ggactttccc accggccgct ccacgctgac gccccggtcc cgccgccgga
     1021 tctcctgcct cagctgcttg ttctccaccc gcaccgcgcg gtactcgctc gcgcggcgca
     1081 tgatggcgac gagatcgtcg ttgccgaacg gcttctggat atagtagtag gcgccggtgt
     1141 tgagggcgtc tatggcgctc cggaggtccg cctgcgccgt catcaggatc actggcgtca
     1201 tcgtgtcctg ttccttggcc gccgcgagga tgtccagtcc gctcaccccc ggcatccgga
     1261 cgtcggtgag gatgatctcg tgatgccccg agcggatctg ctccaggccg gccttgcctc
     1321 cctgggcggt ggtgacctcg aacccctcgt tccgcagcag gatgcgcagg gtatcgagga
     1381 tgccggactc gtcatccacg atcaggacac tgggcttctg gctcatgccg actcctctcc
     1441 cgcgtcgccg gccgggaggt agatagtaaa ggcggtgccg gcaccggcgg ccgactcgac
     1501 gaagatagtg ccgcggtgcg cctcgacggc gcgctgcacg atggcgagac ccagcccgct
     1561 ccccccaggg cgccggctca cgaacggctc gaagaggcgg gcccggatgt cctccggaat
     1621 tcccggcccc gtgtccttca cctgcagccg caccccccgg ctgatccggg gcatcccggg
     1681 cggaagttcg ttgccggcca gggtcttcag ggcgacggtg acctccaccg gctcgggccc
     1741 cgccacctgg acggcgttga gcaccaggtt ggccagggta cggtgcagca ggtcttcatc
     1801 gccctgcacc acggtgctct cgccctcgac gccgagacgg gtggcggcct tgtggtcggg
     1861 gtgggcactg acgaggcgca ccacggcatc tgccacctcg cggagatcca ccggctccga
     1921 cttggtcacc cgcacccggg aaaaatcgag gaactcggtg agcaggcggc tcagccggtc
     1981 gctctcgcgc acgatgagcc gggccagggt ctgctcgtcc tcgccggcat agctgctgcg
     2041 ggcgagctgc tccaccgagc tccggatgga ggcgagcggg ttcttgatct cgtgggccag
     2101 cgacgcgctg agagccgtca ccgcttccag ccgctcggcc cgcagctgca gctcctcgac
     2161 ccgcttggag tcggagatgt cgctgaagac cgccgtcacc gacggcccgc tctggcccgg
     2221 ctgctgaaat gtggtggtgc tgaggccgat ggaaaactgc cggccgccgc cgttgtccac
     2281 cgcggcctcg ccgcgccgca cccggcggcc ctgttccagc ccctcgacca gcgcgctcca
     2341 gagcccgggg gcgcgactcc gcacgatgtt catcaccggc gcaccgatgg ccggaccgcg
     2401 ctccagcccg agcaggcgct gggccgtggg gttggcgtac gccaggttgc cgccgctgtc
     2461 caccgagagc acgccggagt ggatgttgcg caggatcacg tcggcttcga gccgcacgcg
     2521 ctgcagctcg tactcgaggg tccggtgctc cacgctcgcc tcgcggagcc gctggccgag
     2581 caccgccacg acggcgaaga cgagaaagaa caccaccacc tggagcgcca gcgccaccgg
     2641 cgccacgcca ccccgatacg ccgttaccag gtacacggtg ctcgccagca gggtggtgag
     2701 cgtcccgaag cggaatggaa ggagcaccgc ataggcggcg atgagcagca cgaacagcgc
     2761 cggcccggtg ccgcccgcgg gccccaggaa gtgcaccagg gtaagaacga gaccgagatc
     2821 cgccaggagc tgcacggcga gaaagacagg gcccggctcc cgccgcgccg ataccaccac
     2881 ccacacaccg tacgccgtga cgaccagtgc caggatgatt cccacactgg caaggaagga
     2941 gacctgcggt gcttcggtcc atgccaggcc cgcggccacc agcaccgcga ccgccagggc
     3001 caggcgtccc gcgaagagcc aggtcagcag ccggcggcgg tccggcaggc gggagcgggc
     3061 cgcggcacgg tcagcgtcgt tcacgcgccg cctgacgcat gggtgctgtc cgcggagctg
     3121 gctggagctc cggggccgtc gggcggccgg ccggcgttgc cgtccgcccg cggatgctgg
     3181 atgttcgcct cgcccggctt cttcacgcgg cccgacatcg cggtgggatc ggcgccaccg
     3241 gtcaccgcat cctccgattc caccgtggcc gggtgggact gttccttccc gtagtcgggc
     3301 tcgcaggctg ccagcagcag caccgcggcg ccggccagga acatccgagc tcgcatttgt
     3361 gctccgaaga tggtgagtgg gcgaaacata ggcgggatgg gcggtatggg cgagatgggc
     3421 gggagtggac ggggctaagc gaaggttgtc accccggcgc aagccggggt ccagtgatgg
     3481 gagagatggt caacctggat tccggctttc gccggaatga cgggccactc acatcctgcc
     3541 catcgcgtcc cgcacccgcc gcaccagttc ccccggcgcg aacggcttgg cgagcacggg
     3601 acgggaatcc ggccgctcct tgtcctcatc gccgtaaccg gacatgaaca ccacggcgag
     3661 gtccgcctgc tcgtggcgcg cacgtgccgc cagttcggcg ccggtcatgc cgggcagcac
     3721 caggtcggtc accagcacgc ctaccggtcc ctcccggcgg agcatcgtga tcgcctcgtc
     3781 cgcatcggcg gcggagacca cgtcgaatcc ggcctcgccc agtgcacggc tcaccagctg
     3841 ccgcacgtcc ggatcgtcct ccaccaccag caccgatccg ctgaccttcc cggcaggcac
     3901 ttcgggaatg accgccggcg gcggcgtgag cctggtgcgg ggaaaggcga gcgtgaacac
     3961 cgatccccgg cccggggcgc tccgcacccg cacctgtccg cggtgctgtg ccacgatgcc
     4021 gtagaccacc gacagcccga gcccggtgtt gtggctgctg cggcgggtcg agaagaacgg
     4081 ctcgaagatg cgtgcctgcg tctcgtcgtc catgccgtgg ccggtatcct cgacctcgac
     4141 gagggcgagg ccgccggtcg cggggcgccg ggtccggagc gtgagccggc cgccctcggg
     4201 catcgcatcc ttggcgttga gcgccaggtt gagcagcgcc tggtcgagct ggccccggtc
     4261 cacgctgacg cctcccgcat cgggcgccag ctcgagcacg agcgcgatgt tctcgcccag
     4321 taccgcccgc agcatcgcgg ccagctcgtc aaccgccgcg ttcaggctga cggccgtcac
     4381 cgccaccggc ccccgccggc cgaacaccat gagctgctgg gtgagcgccg ccgcccgctc
     4441 cgacgccttc cggatctcca ccgcgtcgct gcgccgcggg tcgtcctcct ccagcgatcc
     4501 gctgaggaag tccgcgaacc ccatgatcac catgagcagg ttgttgaagt cgtgcgcgac
     4561 gccc
//
LOCUS       DASUAP010000277         5710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_894162, whole genome shotgun sequence.
ACCESSION   DASUAP010000277 DASUAP010000000
VERSION     DASUAP010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894162"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1019)
                     /locus_tag="VFU45_07345"
     CDS             complement(81..1019)
                     /locus_tag="VFU45_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEU5358916.1"
                     /translation="MPHAHVHGLHHVTCIAGDPQENLDFYTKVLGMRLVKKSINQDAP
                     DTYHLFYADAAGSPGTDLTFFPWPDAAPGRPGVGLTNEVMLAVPAGTLPWWRARLDEA
                     KVRRGEEVTRFGEKTLPFTDPHGLELALVEIAEDRPFAPWDKSPVPADKQIRGFHAVR
                     LWERDPAPTVRLLVDVMGLAPVGNDDGWQRFGIGGRSGTLIDVKAFPDERRGAWGLGS
                     VHHIAWRVTDTPEEMAVRSNVEGAGLHPTPQIDRFWFQSVYFKEPGGALFELATDGPG
                     FARDEDPAHLGETLVLPPWYEPRRAEIEAGLPALRA"
     gene            complement(1170..2252)
                     /gene="rlmN"
                     /locus_tag="VFU45_07350"
     CDS             complement(1170..2252)
                     /gene="rlmN"
                     /locus_tag="VFU45_07350"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU5358917.1"
                     /translation="MATAPVKAGATPTGILDLPPERAREKLTKWVQAQGLPAYRVNQI
                     VPRLWQRPLAAWSEASDLPLTIREALERDHPLPRLALDVAQHSQDGTKKFLWRLPDGE
                     AIESVLIPSGTRRTLCISSQSGCALGCVFCATGTMGFRRNLTAYEIAGQVREIVLRDP
                     SDAPTNVVFMGMGEPLLNWPAVNAALTILNHPEGFGIGARHITVSTVGIIPGMEQLAK
                     RPEQFRLAISLHAPTAERRLAIMPVQKKYGLEEVLQAAEEFKKRITFEYVLIEGKNDS
                     NKDADALAKLARRMGAHVNLLPLHPGGAPGLSPTSSDRIREFAARLVNQGVEATVRRS
                     RGLDINAACGQLRVEVEKKRALRAEG"
     gene            2516..2998
                     /locus_tag="VFU45_07355"
     CDS             2516..2998
                     /locus_tag="VFU45_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5358918.1"
                     /translation="MTITATWTCVLLTDLLHPDRVRVPLAGRDKPAILRELSRLLAER
                     TGGQEEDILRAVEEREAVLSTGIGYGVAIPHGRAATVPELSLVAGSTPVGVDFDSVDG
                     LPVRLFFLLVGPENAAGQHVKALSRIARVVRRDQVRERLLQAATATEFIQTIVDAEER
                     "
     gene            2995..4770
                     /gene="aspS"
                     /locus_tag="VFU45_07360"
     CDS             2995..4770
                     /gene="aspS"
                     /locus_tag="VFU45_07360"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU5358919.1"
                     /translation="MTAESTSLRTHRAGSLTRAHVGQTVRLGGWVHRTRDLGGLLFID
                     LRDRDGLVQLSFDPRWTPPAVMTAAAQVGSESVVLVQGTVELRPEPAPDRSMTSREVE
                     VHVTTFAIVGPAMTPAIPVARKEGEELAAEDLRLKHRVLDLRRPELQRNLQLRHRLLQ
                     RARRTMSELGFLEIETPVLTKPTPEGARDYLVPSRVHPGEFYALPQSPQIYKQLLMVA
                     GFDRYFQLARCFRDEDLRADRQPEFTQVDLEASFVTQDDVMGFVEQALVDLWDEAGHR
                     IERPFRRLAWREAMERWGVDKPDLRFPLEITDLTARVGADAAPFVTDALKAGGRLRAL
                     LVPGAGFSRKDFDQLTAIARENRAGGLIWAKRSAAGWEGQGVKAIGEQALRTLDGGEG
                     DALLAVVGPDRVTSPALHGVRLEAIRRAGIKPVTDHAFCWVVDFPLFEKDEATGEWVF
                     THHPFTSPHPDDAAFFESDPGRCRALHYDAVYNGNELGSGSIRITDPAVQGAVFRKLG
                     VSAEEQRRRFGFLLDGLASGAPPHGGFAIGFDRVAMLLSAAPSLRDVIAFPKTTAARA
                     LFEGAPTTVDGRDLEALHIQVRSER"
     gene            4938..>5710
                     /locus_tag="VFU45_07365"
     CDS             4938..>5710
                     /locus_tag="VFU45_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU5358920.1"
                     /translation="MTDDLTHRLSTEGADPLLLAGVNDANLLELQRSLGVRVSLRGDA
                     VSVSGTLEQVERATPVLQGMIDSARMGEAVTVEDVHRYAADGLPPEHDASGDQRIVLP
                     GTRKAIAPKTQGQKDYVQAIGVHDIVVGVGPAGTGKTYLAVAKAVEALVRKRVRRIIL
                     ARPAVEAGESLGFLPGDLQAKVDPYLRPLYDALEDMMPHDKVQKALETRTIEIAPLAY
                     MRGRTLADAFIILDEAQNATGAQMKMFLTRIGVNSKVVVT"
BASE COUNT          831 a         2004 c         2005 g          870 t
ORIGIN      
        1 gccggggctg gtcgctcagc cgagacatgc cgacgccgga tgggtcgtcg acgtgtaccg
       61 ggtcgggatg gattgaggcg ctaggcgcgg agcgccggga gccccgcctc gatctccgcg
      121 cgtcgcggct cgtaccatgg aggcaggacc agcgtctcgc cgaggtgggc cggatcctcg
      181 tcgcgggcga accccgggcc gtcggtggcg agctcgaaga gcgccccgcc cggctccttg
      241 aagtacaccg actggaacca gaaccggtcg atctgcggcg tcgggtggag ccccgctccc
      301 tccacgttgc tccgcaccgc catctcctcg ggcgtgtccg ttacccgcca cgcgatgtga
      361 tgcaccgagc ccagtcccca ggcgcctcga cgctcgtcgg ggaacgcctt cacgtcgatc
      421 agcgtccccg accttccgcc gatcccgaag cgctgccaac cgtcgtcgtt gccgaccggg
      481 gccaggccca tcacgtcgac gaggagcctg accgtcggcg ccgggtcgcg ctcccagagc
      541 cggacggcgt ggaagccgcg gatctgcttg tcggcgggga cggggctctt gtcccacggg
      601 gcgaacggac ggtcctcggc gatctccacc agcgcgagct cgagtccgtg cgggtcggtg
      661 aaggggagcg tcttctcccc gaagcgcgtc acttcctcgc ctcgtcgtac cttcgcctca
      721 tccagccggg cccgccacca ggggagtgtt ccggccggca ctgccagcat cacctcgttg
      781 gtgaggccga cgcccgggcg gccgggggcg gcgtcgggcc aggggaagaa ggtcaggtcg
      841 gtgcccgggg agcccgcggc gtcggcgtag aagaggtgat aggtgtcggg ggcgtcctgg
      901 ttgatcgact tcttgaccag gcgcatcccg aggaccttgg tgtagaagtc gaggttctcc
      961 tgcgggtcgc cggcgatgca ggtgacgtgg tggaggccgt ggacgtgtgc gtggggcatt
     1021 atggtcttct cgtgagaacg gtgtcatcct gagggcccgg cagggcccga aggatctctc
     1081 agtcagtaac acccgccggt tcccgcctct cgcatcccgg gcatcgggtg tggccgggag
     1141 agggcaacgg tcatctaagc cctaagcgct cagccctcag ccctcagcgc ccgcttcttc
     1201 tccacttcca cccggagctg cccgcacgcc gcgttgatgt cgagtccgcg gctccgcctg
     1261 accgtcgcct cgacgccctg attgacgagc cgggccgcga actcgcggat ccggtcggaa
     1321 gatgtcggtg agagcccggg tgctccgccg ggatggagcg gcaggaggtt cacgtgcgcc
     1381 cccatccgcc gcgccagctt ggcgagcgcg tcggcgtcct tgttcgagtc gttcttccct
     1441 tcgatgagca cgtactcgaa ggtgatccgc ttcttgaact cctccgccgc ctggagcacc
     1501 tcttcgagcc cgtacttctt ctgcaccggc atgatcgcca gccgccgctc ggcggtgggg
     1561 gcgtggagcg agatggccag ccggaactgc tccgggcgct tggccagctg ctccatcccg
     1621 gggatgatcc ccaccgtgct caccgtgatg tggcgcgcgc cgatgccgaa cccctcgggg
     1681 tggttgagga tggtgagcgc ggcgttcacc gccggccagt tgaggagcgg ctcccccatc
     1741 cccatgaaca cgacattggt cggtgcgtcg ctcgggtcgc ggaggacgat ctcccgcacc
     1801 tggcccgcga tctcgtacgc cgtgaggttc cgccggaacc ccatcgtccc ggtggcgcag
     1861 aagacgcagc cgagggcgca tcccgactgc gaggagatgc agagggtgcg gcgggtgccg
     1921 gaggggatga ggaccgactc gatcgcctcg ccgtccggga ggcgccagag gaacttcttg
     1981 gtgccgtcct gcgaatgctg cgccacgtcg agcgccaggc ggggaagggg atggtcccgc
     2041 tcgagcgctt cgcggatggt cagggggagg tcgctcgcct cgctccacgc cgccagcggc
     2101 cgctgccaga gtcggggcac gatctggttg acccggtacg ccggaagccc ctgggcctgg
     2161 acccatttgg tgagcttctc gcgcgcgcgc tccggaggaa ggtcgaggat cccggtcggt
     2221 gtcgcgccgg ctttgacggg tgcagtggcc atgggcggaa gatactggct cgtgacgacg
     2281 aagtgggagc acttcgtgcc atgagagtgg gaccacggag gtcacggagg ccacggagag
     2341 gcacggagca aagggacgta gtgcgggtcg agcatgccat gctccgcacg cgaggccacc
     2401 gcaaacgcgg ttctccgtgc atctccgctc tgctccgtgc cctccgtggt cccaccttca
     2461 tcgcgtaact gcttgaaatt gaattgcttg ccgcgccact aagccgccgc taactttgac
     2521 catcaccgcc acctggacct gcgtgctcct gaccgacctt ctgcaccccg accgggtgcg
     2581 cgttcccctc gcgggccggg acaaacccgc catcctgcgg gaactgtcgc ggctcctggc
     2641 ggagcggacc ggtggacagg aggaggacat cctgcgcgcc gtggaagagc gggaagcggt
     2701 gctgagcacc ggcatcgggt acggggtcgc gatcccgcac ggtcgcgccg cgacggtgcc
     2761 ggagctgagc ctcgtggcgg gttccacccc ggtgggtgtc gacttcgatt cggtcgacgg
     2821 cctgccggtc cgtctcttct tcctgctggt cggccccgag aacgccgcgg gccagcacgt
     2881 gaaggcgctg tcccggatcg cccgcgtggt gcggcgcgac caggtgcgtg agcggctgct
     2941 gcaggccgcc accgccaccg aattcatcca gaccatcgtc gacgccgaag agcgatgacc
     3001 gccgaatcca cgtcccttcg tacgcaccgc gccggatcgc tcacccgcgc ccacgtcggc
     3061 cagacggtcc gcctcggcgg gtgggtccac cgtacccgcg acctcggcgg gctcctcttc
     3121 atcgacctgc gcgatcgcga cggcctggtg cagctctcct tcgacccgcg ctggacgccc
     3181 cccgccgtga tgaccgcggc agctcaggtc ggcagcgagt cggtggtgct ggtgcagggc
     3241 accgtcgaac tccgccccga gcccgcgccc gaccggtcca tgacctcccg cgaggtcgag
     3301 gtccacgtga cgacgttcgc catcgtcggt cccgcgatga cgccggccat tcccgtggca
     3361 cggaaggaag gggaggaact cgcggccgag gacctgcgcc tcaagcatcg ggtgctcgac
     3421 ctgcggcgtc ccgagctcca gcggaacctc cagctgcgcc accggctcct ccagcgcgcc
     3481 cgccgcacga tgagcgagct cggcttcctc gagatcgaga cgccggtcct caccaagccg
     3541 acgccggagg gggcgcgcga ctacctggtg ccgagccggg tgcatccggg tgagttctac
     3601 gcactacccc agtcgccgca gatctacaag cagctgctga tggtggcggg gttcgaccgc
     3661 tacttccagc tggcgcgctg cttccgcgac gaggacctcc gcgccgaccg ccagcccgag
     3721 ttcacccagg tggacctcga ggcgtcgttc gtgacccagg acgacgtgat gggcttcgtg
     3781 gaacaggcgc tggtcgacct gtgggatgag gccgggcacc gtatcgagcg cccgttccgt
     3841 cggctcgcct ggcgcgaggc gatggagcgc tggggcgtgg acaagcccga cctgcgcttc
     3901 ccgctcgaga tcaccgacct cacggcgcgg gtcggcgccg acgccgcacc cttcgtgacc
     3961 gatgccctca aggccggcgg gaggctccgc gcgctgctgg tgcccggcgc cgggttcagc
     4021 cggaaggact tcgaccagct caccgccatc gccagggaga accgggccgg cgggctcatc
     4081 tgggccaagc gctcggcggc ggggtgggag gggcagggcg tcaaggccat cggcgagcag
     4141 gcgcttcgga cgctcgacgg cggcgagggc gatgcgctcc tggccgtggt cgggcccgac
     4201 cgcgtgacct cgccggcgct ccacggcgtg cgcctcgagg cgatccgccg cgccgggatc
     4261 aagcccgtca ccgaccacgc cttctgctgg gtggtcgatt tcccgctctt cgagaaggac
     4321 gaggccaccg gcgagtgggt ctttacccac cacccgttca cctccccgca tcccgacgat
     4381 gcggccttct tcgagtccga tccggggcgc tgccgcgcgc tccactacga tgcggtgtac
     4441 aacggcaacg agctggggag cggatcgatc cggatcaccg atcccgcggt gcagggggcg
     4501 gtcttccgga agctcggcgt gtccgcggag gagcagcgcc ggcgcttcgg cttcctcctc
     4561 gacgggctcg cctccggcgc gcccccccac ggcggcttcg cgatcggctt cgaccgggtg
     4621 gcgatgctcc tctcggcggc accctcgctg cgcgacgtga tcgcgttccc caagaccacc
     4681 gccgcgcggg ccctgttcga aggcgcgccg accaccgtcg acggacgcga tctcgaggca
     4741 ctgcatattc aggtgagaag tgagcggtaa gacgtgagcg ggctgcgcga cgggatctcg
     4801 aagggtggcc ggcgcacggc tcacctttca ccgctcaccg aaagggagcg ggaagcggtg
     4861 agcgggctgc gcgacgggat ctcgaagggt ggcccgctca cggctcaccg ctcaccgctc
     4921 accgacaagg agcatcgttg accgacgact tgacccaccg cctctcgacc gaaggcgccg
     4981 acccgctcct gctggccggc gtcaacgacg ccaacctcct cgagctccag cgctcgctcg
     5041 gcgtgcgtgt cagcctccgg ggtgatgccg tgagcgtgag cggcaccctc gagcaggtcg
     5101 aacgggcgac accggtgctg caggggatga tcgacagcgc gcggatgggg gaggcggtga
     5161 ccgtcgagga cgtgcaccgc tacgccgccg acggcctgcc gccggagcac gacgccagcg
     5221 gcgaccagcg gatcgtcctg cccgggaccc ggaaggccat cgcccccaag acccaggggc
     5281 agaaagacta cgtccaggcc atcggcgtcc acgacatcgt ggtgggcgtg ggtcccgccg
     5341 gcaccggcaa gacgtacctc gcggtggcca aggcggtcga ggcgctggtg cggaagcggg
     5401 tgcgccgcat catcctcgcg cggccggcgg tggaggcggg ggagtccctc ggcttcctcc
     5461 ccggcgacct gcaggccaag gtcgatccgt atctgcggcc gctgtacgat gcgctcgagg
     5521 acatgatgcc gcacgacaag gtccagaagg cgctcgagac ccgcaccatc gagatcgcgc
     5581 cgctcgcgta catgcgcggt cgtacgctcg ccgacgcgtt catcatcctc gacgaggcgc
     5641 agaacgccac cggcgcgcag atgaagatgt tcctcacccg catcggggtg aacagcaagg
     5701 tcgtcgtcac
//
LOCUS       DASUAP010000278         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_921491, whole genome shotgun sequence.
ACCESSION   DASUAP010000278 DASUAP010000000
VERSION     DASUAP010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921491"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VFU45_07370"
     CDS             <1..1195
                     /locus_tag="VFU45_07370"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEU5358921.1"
                     /translation="FEVERVFAAVDILERTADGVRITEVKSTTKLKDEHIPDIAVQRW
                     VVEGTGLPVAATRLMHLNRDCRAPDLSNLFATAPVDDLVTPVLPTVEPEVQAQLRMLA
                     GPLPDVAIGDHCTKPYDCPFLDRCWPKLPPHLISTLPRLSAKKRTALQVLGVETIDRI
                     PDDFELTDIQERQRRAVKAGRPLIEPELRTELAAFAGDRIAFLDFETVAPAIPVWDGC
                     RPFDAVPAQVSVDVVEAGAVLDHHEWMASDAGDPRPMIAQTVVAACAGADVVVAFNAG
                     FERRCLELVAEAVPEHAPAINAIIGRLRDLADPVRRGIYHPDFHGSYSLKAVIPALVP
                     ELAYHDLEVAEGTTASTRLAALMFTPERFSAEERERTRRHLLEYCARDTLVMVRLLDT
                     LRSLA"
     gene            1341..1808
                     /locus_tag="VFU45_07375"
     CDS             1341..1808
                     /locus_tag="VFU45_07375"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HEU5358922.1"
                     /translation="MTPLADGRTGVVMRDFGYEVSVEGSGDTIDVAIGFQTDFASVPR
                     PLWWLFPCWGKYGNAAVVHDWLYWTQTRPKPAADRIFLEAMTVLGVGGFTRYSLYYAV
                     HWFGFAAWVQNREDRAQGYLRVQPVAGLKATATSERRNKWAQVGRAMIRKFRP"
     gene            1849..2043
                     /locus_tag="VFU45_07380"
     CDS             1849..2043
                     /locus_tag="VFU45_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358923.1"
                     /translation="MTTIVETGNFASTGFVTFLFGIFTAYWAQTTRRNAWAWFFFGLI
                     LAPIAGLVLLYKNGMARTGT"
     gene            2040..2525
                     /locus_tag="VFU45_07385"
     CDS             2040..2525
                     /locus_tag="VFU45_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358924.1"
                     /translation="MTRGAVATALLVLGAIGGRPLAAQDARYRHHDAALLPDSLETPG
                     AVATTDTARICRKGYSRSVRHVTAGVKRQVYTAYATQPHDSTCCEVDHLISLELGGSN
                     EFQNLWPEPYVPHPGARDKDKVENWLHREVCAGRIPIEEAQREIAEDWYAVYLQLPKR
                     R"
     gene            2800..3276
                     /locus_tag="VFU45_07390"
     CDS             2800..3276
                     /locus_tag="VFU45_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5358925.1"
                     /translation="MALTIRSATAADVPLILRFIRGLAEYERLLDECVATEALLRTSL
                     FGPRPDAEVVIASLDDRPAGFALFFHSYSTFLARRGLYLEDLFVLPEFRGKGVGKALL
                     RHLARLAVDRGCGRFEWAVLDWNESAIEFYESLGAVAMGDWITYRLTGEALARLAR"
     gene            3329..3676
                     /locus_tag="VFU45_07395"
     CDS             3329..3676
                     /locus_tag="VFU45_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEU5358926.1"
                     /translation="MPFAIKRVYDPPASNDGHRILVDRLWPRGVTKTRARVDEWLKEI
                     APSTELRRWFHADPSRWAEFRSRYRHELGDHAEAVAHVRALARKGRVTLLYGARDPEH
                     NHAIVLKEFLGRK"
     gene            3673..3984
                     /locus_tag="VFU45_07400"
     CDS             3673..3984
                     /locus_tag="VFU45_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358927.1"
                     /translation="MTPRSLLTAYCLLLLGPLLPLGAHVRVRVAHDRAWHEGNVVLND
                     YGCLTVVYTDPTGHPTAGVARFPQVLALELADPAGHWRPVPIDSVRFADRTCGTVKHA
                     E"
BASE COUNT          612 a         1518 c         1327 g          598 t
ORIGIN      
        1 gttcgaggtg gagcgggtct ttgcggcggt cgacatcctc gagcgcacgg ccgatggcgt
       61 ccggatcacc gaggtcaagt cgaccacgaa gctcaaggac gaacacatcc ccgacatcgc
      121 ggtccaacgc tgggtggtgg agggcaccgg actcccggtg gccgccaccc gcctgatgca
      181 cctcaaccgc gactgccgag cccccgacct ctccaacctc ttcgccacag caccggtcga
      241 cgatctggtg acacccgtcc tcccgacggt cgagcctgag gtgcaggccc agctccgaat
      301 gctcgcgggg ccgcttcccg acgtcgccat cggcgaccac tgcaccaagc cctacgactg
      361 cccattcctg gaccgctgct ggcccaaact ccccccgcac ctcatctcca ctctcccccg
      421 cctgagcgcc aagaagcgca ccgccctcca ggtactcggc gtcgaaacga tcgaccggat
      481 ccccgacgac ttcgagctca ccgacatcca ggaacgccag cgccgcgcgg tgaaggcggg
      541 acgcccgctc atcgagccgg aactgcggac cgagctcgcc gcgttcgcgg gggacaggat
      601 cgcattcctc gacttcgaga cggtggcccc ggccatcccg gtctgggacg gctgccgccc
      661 cttcgacgcc gtgccggccc aggtgagcgt cgacgtcgtg gaggccggcg cggtgctcga
      721 ccaccacgaa tggatggcga gcgacgccgg cgacccccgg ccgatgatcg cccagacggt
      781 cgtcgcggcc tgcgccggcg ccgacgtggt ggtggcgttc aacgccgggt tcgaacggcg
      841 ctgcctggag ctggtggccg aggcggtgcc agaacacgcc ccggccatca acgcgatcat
      901 cggccggctc cgcgacctgg ccgacccggt gcgccgcggc atctaccacc ccgacttcca
      961 cgggagctac tccctcaagg ccgtcatccc ggcgctcgtc cccgaactcg cgtaccacga
     1021 cctcgaggtg gcggaaggga ccaccgccag cacccggctc gcggcgctga tgttcacgcc
     1081 ggagcggttc tcggccgagg agcgggagcg gacccggcgg cacctgctgg agtactgcgc
     1141 gcgggacacg ctggtgatgg tccgcctgct cgacacgctg cggtcgctgg cctaggccct
     1201 cccacgatca ccggcggccc cgggccgcgg atacgggcgg tccttccggg ggcccggccc
     1261 cgcagctagg ctcagggagc gcacgcgccc gctcgaccgg aaggacccga ccgaggtcgc
     1321 gcttcaccgg ccccctgctc gtgaccccgc tcgccgacgg caggaccggg gtggtgatgc
     1381 gggacttcgg gtatgaggtg agcgtcgagg ggagcggcga cacgatcgac gtcgccatcg
     1441 ggttccagac cgactttgcg tcggtgccgc gcccgctctg gtggctcttc ccctgctggg
     1501 ggaagtacgg caacgccgcg gtggtgcacg actggctcta ctggacccag acccgcccca
     1561 agcccgccgc cgaccggatc ttcctcgagg cgatgacggt gctgggcgtc ggcgggttca
     1621 cccggtactc gctctactac gccgtgcact ggttcggctt cgcggcctgg gtccagaacc
     1681 gcgaggaccg cgcccagggc tacctgcgcg tccagccggt ggcgggcctc aaggccaccg
     1741 cgacctccga gcgccggaac aagtgggccc aggtcggccg cgcgatgatc cgcaagttcc
     1801 gtccgtgaga tggaccgacg gacgttcccc acagcttcgg gagacacgat gaccacgatc
     1861 gtcgagaccg gcaacttcgc cagcaccggg ttcgtgacct tcctcttcgg gatcttcacc
     1921 gcctactggg cccagaccac ccggcggaac gcctgggcct ggttcttctt cggcctcatc
     1981 ctggcgccga tcgcggggct cgtgctgctg tacaagaacg gcatggcgcg gaccggcacg
     2041 tgacgcgcgg cgcagtggcc acggccctgc tcgtcctcgg cgcgatcggc ggccggcccc
     2101 tggccgccca ggacgcccga taccgccacc acgacgcggc gctcctcccc gattccctcg
     2161 agaccccggg cgccgtggcc accaccgaca ccgcgcgcat ctgccggaag gggtacagca
     2221 ggtcggtccg ccacgtgacg gccggggtca agcgccaggt ctacacggcc tacgccaccc
     2281 agccccacga ctccacctgc tgcgaagtgg accacctcat ctccctcgag ctcggcggtt
     2341 ccaacgagtt ccagaacctc tggcccgagc cctatgtgcc ccaccccggc gcgcgggaca
     2401 aggacaaggt cgagaactgg ctccaccgcg aggtctgcgc cggaaggatc ccgatcgagg
     2461 aggcgcagcg ggagatcgcc gaggactggt acgcggtgta cctccagctg ccgaagcggc
     2521 ggtgagcgcg ggaatgaggt gcgcacgacc gggatcgctt ggtgaagtag gtttgttgtg
     2581 ggaccacgga gtacacggag ggcacggaga tacacggaga actgcatgtg atgtggtctt
     2641 gcacaaggag ctccgctcgc gaggccatac tatatcgctt tcctccgtgc ctctccgcgt
     2701 tctccgtgtc cgagcctgcc ctgagcgcag cgaagggtgg tcccatctcc ggcttcacac
     2761 ccgctcccgg ttccacgccc cctcgtttac cgttgaccca tggccctcac catccgttcc
     2821 gccacggccg ccgatgtccc gctcatcctc cgattcatcc gggggctggc cgagtatgag
     2881 cggctgctcg atgagtgcgt cgcgaccgag gcactgctgc gcacctcgct cttcggcccc
     2941 cggcccgacg cggaagtggt catcgcgtcg ctggatgacc gcccggcagg gttcgcgctc
     3001 ttcttccaca gctacagcac cttccttgcc cggcggggac tctacctcga ggacctcttc
     3061 gtcctccccg agttccgtgg caagggcgtc ggcaaggcgc tgctcaggca cctggcccgc
     3121 ctggccgtcg accgcggctg cggccgcttc gaatgggcgg tgctcgactg gaacgagtcc
     3181 gccatcgagt tctacgaatc cctcggcgcg gtcgcgatgg gcgactggat cacctaccgc
     3241 ctgaccggcg aggcgcttgc ccgcctggcc cggtgaacca agtgaccgga agtgggacca
     3301 caacaaacct cgcaacggga gcgcatctat gcccttcgcg atcaagcgcg tttacgaccc
     3361 gcccgcctcg aacgacggcc accgcatcct ggtcgaccgg ctctggcccc ggggggtgac
     3421 caagacccgc gcgcgggtgg acgaatggct gaaggagatc gccccctcga cggagctgcg
     3481 gcgctggttc cacgccgatc catcgcgctg ggcggagttc cggagccggt accggcacga
     3541 gctcggcgac cacgccgagg cagtcgcgca cgtgcgcgca ctcgcgcgga agggccgggt
     3601 gacgctgctc tacggcgcgc gcgacccgga acacaaccac gcgatcgtcc tgaaggagtt
     3661 cctgggccgc aaatgacccc ccgctcactg ctcaccgctt actgcttact cctcctgggc
     3721 cccctcctcc cgctcggcgc ccacgtgcgg gtgcgcgtgg cgcacgaccg cgcctggcac
     3781 gaaggcaacg tcgtgctcaa cgactacggc tgcctcacgg tggtctacac cgaccccacc
     3841 ggccatccga ccgcgggcgt agcgcgcttc ccgcaggtgc tggcgctcga actcgccgac
     3901 ccggccgggc actggcgacc cgtccccatc gactccgtcc gctttgccga ccgcacctgc
     3961 gggacggtca agcacgcgga gtaggctgct ccccctctcc ggcgcgtcag cgctggagag
     4021 ggggccgggg ggtgaggctg tgcgggcggc gcatg
//
LOCUS       DASUAP010000279         4095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_511811, whole genome shotgun sequence.
ACCESSION   DASUAP010000279 DASUAP010000000
VERSION     DASUAP010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511811"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2205)
                     /locus_tag="VFU45_07405"
     CDS             complement(<1..2205)
                     /locus_tag="VFU45_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5358928.1"
                     /translation="MLLALAAPLAAQAAPQDSVSLWLNIPYIMRGAETTGRAPERVKW
                     TPDSKWIYFRWDTAGTPWREERHWFRVRPAAGATPERVSDAAADSVAPLLAEGSLSPD
                     RKSRAVAALGDLWIVDLKAGTTRRLTDTRAKESDPAYAADGRAVLFTRDDNLFRVDLG
                     TGLVSQLTDIRKGPAPKDAKPDTGQRGYLEREQTELFQSVRDRLYADSVRKAAEKARD
                     SLRAAPIYLADDETVQSISASLSGRSALLFTSIPAKGRKPTIVPDWVTASGYVTDITG
                     RENVGDAQESGRVGMIDLPSGKVSWLSLVPGDSTAAPAWANPMGWNDAGTSALIFAVT
                     KDFKRRIIWRVDAADEAHGAHESHKTPVANLSALDSLRDTAWVDGPCFGCGGWLEGGK
                     RAWYVSEADGWAHLYSVAADGSDKRQLTSGRWEVRGAEVSRDGRTFFLHTSEGSPYEQ
                     HFWRMPVSGGAREEVTPRAGWHNASVSPDGRLIADVFSTADRPPEIFVGPSRAGAELA
                     QVTTSPTAEWLKGPWIDPAIITIKASDGVEVPAHIYRPQDLGAKPNGAAVIFVHGAGY
                     LQNVMRGWSDYYSREYQFHHLLAAKGYVVLDLDYRGSAGYGRDWRTAIYRHMGGRDLQ
                     DEVDASRYLQATYGIGPDHVGIYGGSYGGFMTLMALFTEGKHFGAGAALRSVTDWAHY
                     NHGYTAAILNTPTQDSVAYRQSSPIYFAAGLDRPLLMAHGMVDTNVEYQDIVR"
     gene            complement(2345..3142)
                     /locus_tag="VFU45_07410"
     CDS             complement(2345..3142)
                     /locus_tag="VFU45_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358929.1"
                     /translation="MLLIAGCSTSAPVGVVPATARTVVAETDQGFETQDIVVVNSSSV
                     PITVTSLTLRFCRNIRNPCVRVPFQLLVPPHTRKQIATVGPADARAAFSYGYSWTWSA
                     DSSAPQADSSAWRVGLHSFGPVAYGMTLAEVSAALGGPVTFDSRDAWVRGGNCGFIAP
                     GGIPRGAALMVRNGRIERVDVDTASIATESGARVGSTDADILRLYPGRIRAERHSYGA
                     SEVDELVYTGASAPDSGFAIIFEVDGHRVVKFRAGRRRAVDDAQGCE"
     gene            complement(3303..3953)
                     /locus_tag="VFU45_07415"
     CDS             complement(3303..3953)
                     /locus_tag="VFU45_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358930.1"
                     /translation="MQGGEPAPDDEPGVIWDGRLGAAWFLGGVWTFDYPAHRLLFNGP
                     ARTGPSDPRCWEPLGFQVDSAGRRTNIFPRVAAVVAGDTIQFLLDTGARTDVTESAWP
                     RIEPATPRHRAASFIGAARFAEWHTRHPDWPVVEHAEAGSDSSAMIRVPSITVDGQAI
                     GPVWFTERPTHSFATFMSQYTDRPVEGALGGSAWQYVTLILDYPRARMAVLAHPQS"
BASE COUNT          665 a         1400 c         1412 g          618 t
ORIGIN      
        1 ccggacgatg tcctggtact ccacgttggt gtcgaccatc ccgtgcgcca tcaggagcgg
       61 ccggtcgagc ccggcggcga agtagatcgg cgaggactgc cgatacgcca cggagtcctg
      121 ggtgggcgtg ttgaggatgg ccgcggtgta gccgtgattg tagtgcgccc agtcggtcac
      181 cgagcggagc gccgccccgg cgccgaagtg cttcccctcg gtgaagagcg ccatcagcgt
      241 catgaagccg ccgtaactcc cgccgtagat cccgacgtgg tcggggccga tgccgtacgt
      301 ggcctggaga taccggctgg cgtcgacctc gtcctgcagg tcgcgcccac ccatgtggcg
      361 gtagatggcg gtgcgccagt cgcggccgta gccggcggag ccccggtagt cgaggtccag
      421 gaccacatag cccttggcgg ccagcaggtg gtggaactgg tattcacgcg agtaatagtc
      481 ggaccacccg cgcatcacgt tctgcaggta accggcgccg tggacgaaga tgaccgccgc
      541 gccgttgggc ttggcgccca ggtcctgcgg ccggtagatg tgcgccggga cctcgacccc
      601 atcggacgcc ttgatggtga tgatcgctgg gtcgatccac ggacccttga gccactccgc
      661 cgtcggcgac gtcgtcacct gcgccagctc ggcgccggcg cggctcggcc cgacgaagat
      721 ctccggcggc cggtcggcgg tcgaaaagac gtcggcgatg agcctgccgt ccggcgacac
      781 cgacgcattg tgccagcccg cccgcggcgt cacctcctcg cgggcgccgc cgctcaccgg
      841 catccgccag aagtgctgct cgtagggcga cccctcgctg gtatggagga agaacgtccg
      901 cccatcgcgg ctcacctccg cgccacgcac ctcccaccgg ccgctcgtga gctggcgctt
      961 gtcgctgccg tcggcggcca cggagtagag gtgggcccag ccgtcggcct cggagacgta
     1021 ccacgcccgc ttgcctccct ccagccatcc gccgcacccg aagcaggggc cgtccaccca
     1081 cgccgtatcg cgaagggagt cgagagcact caggttcgcc acaggcgtct tatgcgactc
     1141 atgcgcccca tgcgcctcat ccgccgcatc gacgcgccag atgatccgcc gcttgaaatc
     1201 cttcgtcact gcaaagatga gcgccgaggt gccggcgtcg ttccatccca tcgggttggc
     1261 ccaggcgggc gccgcggtgg agtcacccgg cacgagcgag agccagctca ccttgcccga
     1321 ggggaggtcg atcatcccca cccgtcccga ctcctgggcg tcacccacgt tctcccgccc
     1381 cgtgatgtcc gtgacgtacc ccgaggcggt gacccagtcg ggcacaatgg tcggcttccg
     1441 gcccttggcc gggatgctgg tgaagaggag cgccgagcgc cccgagagcg aggccgagat
     1501 cgattgcacc gtctcgtcgt ccgccaggta gatgggcgcg gcgcggagcg agtcccgcgc
     1561 cttctccgcc gctttccgca cgctgtcggc gtagagccgg tcgcggaccg actggaagag
     1621 ctcggtctgt tcccgctcga ggtagccgcg ctggccggtg tcgggcttgg cgtccttggg
     1681 tgccggtccc ttccggatgt cggtgagctg ggacacgagc ccggtgccga ggtcgacgcg
     1741 gaagaggttg tcgtcgcggg taaagaggac cgcgcgtccg tcggcggcgt aggcgggatc
     1801 cgactccttg gcgcgggtgt cggtgaggcg gcgggtggtc cccgccttga ggtccacgat
     1861 ccagaggtcg ccgagtgcgg cgactgcgcg gctcttccgg tcaggggaga ggctcccctc
     1921 cgccaggagc ggcgccacgg aatcggctgc ggcgtcgctc acgcgctcgg gagtggcacc
     1981 ggccgcgggc cgcacccgga accagtggcg ctcttcccgc cacggcgtcc ccgccgtgtc
     2041 ccagcggaag tagatccact tcgagtccgg cgtccacttc acccgctcgg gcgctcggcc
     2101 ggtggtctcc gcgccccgca tgatgtaggg gatgttgagc cagagcgaca ccgagtcctg
     2161 cggcgcggcc tgggcggcga ggggcgcggc gagggcgagg agcatcagtg gagagaagcg
     2221 catggagacc gtgagaggtg agacgtgaga ggtaagacgt gagaggtgag acgtgagacg
     2281 taagccggaa gacccgcgac ccgcgaccac tgcgcacaga cagccgcatg cgctagggcg
     2341 gcggtcactc gcatccctgc gcgtcatcga ccgcccggcg tcggcccgct cggaacttca
     2401 cgacgcggtg accgtcgacc tcgaagatga tggcgaaccc ggaatctggt gcgctcgcgc
     2461 cggtgtagac gagctcgtcg acctccgatg caccatagct atggcgctcg gcccggatgc
     2521 ggccgggata cagccgaagg atatccgcat cggtcgatcc gacacgcgcg ccactctccg
     2581 tggcgatact cgcggtgtcg acatcgaccc gctcgatacg cccattgcgc accatcaatg
     2641 cggcgccgcg gggaatccca cccggcgcaa tgaagccaca gttcccgccg cgcacccagg
     2701 catccctcga gtcgaatgtc accggaccgc cgagcgcggc ggacacctcg gcgagcgtca
     2761 tgccatacgc gacgggaccg aaggaatgaa gacccacccg ccacgccgac gaatcggcct
     2821 gcggcgccga cgagtccgcg ctccacgtcc acgaatatcc gtaggagaac gcggccctcg
     2881 catcggccgg cccgacggtg gcgatctgct tgcgcgtgtg cgggggtaca aggagctgga
     2941 acgggacccg gacgcacgga ttccggatgt tgcgacagaa gcgcagggtg aggctcgtga
     3001 ccgtgatcgg caccgacgag gagttgacca ccacgatgtc ctgggtctcg aacccctgat
     3061 ccgtctcggc aacgacggtc cgtgcagtgg ccggtacgac gccgaccggg gcgctcgtgc
     3121 tgcagccggc gatgaggagc aaaggaatta cgcgcagcgg gacccgtccc gaggacggcg
     3181 acctcgaacg ccggctcagg gagttgggat caatgccgtc cactctggcc cccctgccca
     3241 tcgatctgtt cacggacctg tggcccggac ggcccgacag ctcggagcag atgccatgga
     3301 ctttaacttt gcggatgggc aagcactgcc attcgcgccc gcgggtagtc gaggatgagc
     3361 gtgacatatt gccacgccga gccgccaagt gcgccctcga ccggccgatc cgtgtactgg
     3421 gacatgaatg tcgcaaatga atgggtcgga cgctccgtga accagactgg cccgatggcc
     3481 tggccgtcga cggtgatcga cggcacacgg atcatggcgg agctgtcgga accggcctca
     3541 gcgtgctcca cgaccggcca atcagggtgc cgggtgtgcc attcggcgaa gcgcgccgca
     3601 ccgataaagc tggccgcgcg gtgtcttggc gtggccggtt cgatgcgcgg ccacgcgctc
     3661 tcggtcacgt cggtccgcgc gccggtgtcg agcaggaatt ggatcgtatc gcctgcgacg
     3721 acggccgcga cccgcggaaa gatgttggtg cgtcggcctg ccgaatcgac ctggaaaccc
     3781 agtggctccc agcaacgcgg atcacttggt cccgtacggg cggggccgtt gaacaacagc
     3841 cgatgcgcgg ggtaatcgaa cgtccatacc ccgcctagga accaagccgc accgaggcgt
     3901 ccgtcccaga tgaccccggg ctcgtcatcc ggcgccggtt cgccaccctg catcaggaac
     3961 tgggcagacg aggagtcccg agttgggatt gaggggacca cgggtcgccc tccgacgccg
     4021 gcactttcac aacgaacatc tcaccatgtg cgcttccgtg cgcccagcgc accgtgtcga
     4081 taggtgcacc gatgc
//
LOCUS       DASUAP010000280        18091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_553585, whole genome shotgun sequence.
ACCESSION   DASUAP010000280 DASUAP010000000
VERSION     DASUAP010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 18091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18091
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553585"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..883
                     /locus_tag="VFU45_07420"
     CDS             98..883
                     /locus_tag="VFU45_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358931.1"
                     /translation="MKDRYGIELCGVGKAYGDRVVLSRLDLNVARGEVVALVGPNGAG
                     KSTTLRILSGAIHPSVGVARIGGRDVTLDPLGAREWLGYLPQRLGVPLSTVVRDLAAL
                     VSNSRALPADAGARALAEAHLGKRLDATLGQMSGGQRQRVMLVLATLGAVEALLLDEP
                     SISLDTEGAEEVRDAISAARFDGAAILFASHHLSDVARLADRIAVMVEGRVVACGTVE
                     ELARRAGVAWDPREPEAPIERIYRNLIQGVPAPVPMALTLVGA"
     gene            880..1692
                     /locus_tag="VFU45_07425"
     CDS             880..1692
                     /locus_tag="VFU45_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5358932.1"
                     /translation="MTRGALIGFEWAAARRGRVVLFFAIGFALASIGVSLAGLSAGGI
                     IAVQGFSRTSVSLLQLVLWTVPLLALLLGAIHGAECYELEFLAGLPAPRSSLVFGRWA
                     AWTAALGTALIGGFGAAGVVIGLFAGAADVLRYLALVGISLLLLSAGLALGLWIGVAA
                     GTRPRAIGAAVIVWFVLAIGLDLTAIAVLAVLPATGATWNLAVLLLANPVDSARALAL
                     GVFSADALAGPTGASLRHLLGGWGVLPLTLSLAVWTAGPLYIAGKTFNNKDL"
     gene            1870..2829
                     /locus_tag="VFU45_07430"
     CDS             1870..2829
                     /locus_tag="VFU45_07430"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5358933.1"
                     /translation="MAIGAFRNAQPQRVQNVDFAQTPVSTDPTYPATVAPAGDAPVKA
                     FRIPIRDAKVEIAAGVTYAGWTFGGTVPGPAIRVRQGDRVRVTVVNQASMPHSIDFHA
                     ARVPMNVDFRMIMPRDSISFEFVARDPGASLVHCGTPPVLLHLMQGMYLPIIVDPKGG
                     WPGKADKEFVLVQSEFYVQGGDSAQPGQPDWQHALDRQASYVVFNGKAFQYQSTPLQV
                     NVGDRVRFFVVNAGPSFTSDFHIVGAIFDRVYPDGDPGHALAGVQTWGIPAGSGAVFE
                     THFAADGSGAGTYAFVTHAFADAAKGAVGLIHVGDGPAVAMAH"
     gene            2830..3249
                     /locus_tag="VFU45_07435"
     CDS             2830..3249
                     /locus_tag="VFU45_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358934.1"
                     /translation="MPVLTRWYVKTAILYLVVALLLGAVLAGNGVVPLPAFLLVAGPA
                     QIHLFVVGWLTQLIFGIAYWMFPKATAQQPRGNDALAVLTYVCLNFGLLARVIGEPWH
                     AVAPGVLAGGLLVASGVLQWLGGVGFAVLAWPRVRGR"
     gene            3263..3664
                     /locus_tag="VFU45_07440"
     CDS             3263..3664
                     /locus_tag="VFU45_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358935.1"
                     /translation="MSIWMVRSALVALVLGFSIGAIMLIGKGTGAFGGLSAGIPIHVE
                     LVVLGWMVQLAMGVALWILPRFGARGPARGETWGWAAWALWNSGIVFVIVGAAGAPAV
                     RLIGRLIELGAAIAFGAAVWSRVRASGISAM"
     gene            complement(3678..4835)
                     /locus_tag="VFU45_07445"
     CDS             complement(3678..4835)
                     /locus_tag="VFU45_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU5358936.1"
                     /translation="MTTTRKRILVLAAGAGLLGGAFFLFRPAPAAVDLTTVTRGPLEV
                     TIDEEGQTRVVDRYTITAPVAGRVARIQAREGDPVQPGTIVAWIFPSPLDARAHEQAA
                     ARVAEAEDAQRAADAVAGQARAAYDQARRSCDRAQELAAQHLIAPEERERAELDETNR
                     QRELESAQFRAQAAAHDVEVARSALLGGAREAVALRSPVRGQLLRVPDPSERVVAPGT
                     PLLEIGDPRNIEIVTDLLSSDAVRVHPGDEMRVEGWGGDSALMGHVIRVEPSGFTKVS
                     ALGVEEQRVNVVGILNTVPAGLGDRFRVDVQIVVWRSPGVLQVPASALFRLGPSWAVF
                     QVENGRVHQRSVEVGHRSGFDVEVTGGLKAGATVIRDPGDQVVEGGRVRAR"
     gene            complement(4840..7212)
                     /locus_tag="VFU45_07450"
     CDS             complement(4840..7212)
                     /locus_tag="VFU45_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5358937.1"
                     /translation="MTRLSLLDRKLVRDLWHLRGQVAAISLVVACGVAVVVTTRTSYT
                     SLGASRAAYYRDYRFADVFAHLKRAPEAVAGRVAAVPGVSAIDTRVVTAVTLDVPGLA
                     EPASGRLISVPAAGRPRLNDVHLRRGRWIEADRPDEAIVSEAFATANHLELGATLGAV
                     INGRWQRLRVVGVGISPEYVYEIGEAQLFPDSRRFGVLWMNRAVLAAAFDMTGAFNDV
                     SIRLAPGASEADVITRVNRILAPYGGLDAYGRADQVSARFLNDELNQNRVSGTLVPAI
                     FLGVAAFLLNIVLTRLVGIEREQIGVLKAFGYENRTVALHYLKLALVALALGAALGIG
                     LGLWFAERVNLNYSRFYRFPVFVFRLDWAAVALAVGVTLLAALAGATGAVRRVVQLPP
                     ATAMQAAAPPRFRPGLLERLGLHRLLSPPTRIMARDLERRPLRSVLSMTGIAFAAAIL
                     IVGRYFVDAMWTIADVQFNRVQREEFTVAFNRPRQPDAVFAARALPGVLSAEGFRTVP
                     ARVSNGHRSRRIPVLGLEPESEMHRLVGTGWRPVRLAPGGLVLTAKLGDILGVRAGDS
                     VSVEVLEGSRPRRDVVVEGFVDELVGLTGYMDRRSLASLLGEDQTVSGVFLRTDARDL
                     AALNARLKALPAVAGVADRRSSLGSFETTLAQSIGIVTVVFVGFAAALAVATVYNAAR
                     LSLSERARELASLRVLGFTRWEVARMLLSEQAVLTLLGTGAGLAIGYGFCALFSDLYQ
                     WEVFRLPLVVSAATYLNAALVVLAAAAVSALLIRRRLNRLNLVEVLKTRE"
     gene            complement(7209..7964)
                     /locus_tag="VFU45_07455"
     CDS             complement(7209..7964)
                     /locus_tag="VFU45_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5358938.1"
                     /translation="MTAATALPQLGTTALAGRDVVFRALGLSKMYRLGDVEVPALRAV
                     DLELYRGEFVVILGPSGSGKSTLLNILGGLDAPTGGRVHFLDHDLTGASDRELTRFRR
                     EHVGFVFQFYNLIPTLTARENVALVTAIATNPMDPAEALRLVGLGERLDHFPAQLSGG
                     EQQRVAIARAIAKRPDALLCDEPTGALDYETGKIVLEVLDQVNRELGTTTAVITHNAS
                     IAAMADRVIRLRSGTITELSVNQRRSRPAELSW"
     gene            8175..9323
                     /locus_tag="VFU45_07460"
     CDS             8175..9323
                     /locus_tag="VFU45_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEU5358939.1"
                     /translation="MFFPQTHPWTEGQSLTDYLEQRGVNRRDFLTWCAEMAAVLGLSS
                     VVTPRVAEAMVAQKRPSVIWLQLQECTGCVESVLRTEAPTIGDLILDVVSLDYQHTLM
                     AGAGKAVEDALRTAEEKNRGQYLLIVTGSIPLNDNGIYCTIGGRTAKEILLEAGRGAK
                     AVVAIGACAHWGSVQAAKPNPTGAVGVADVLKDRPIVNIAGCPPIADVVTATIVHYLT
                     FGRLPATDYDGRPLFAYGARIHDQCSRRANFDAGQFVEKFDDDAARRGWCLYHVGCKG
                     PETFSPCPIFLWNDKTSWPIGAGHPCLGCTERHFWDTMTPFYGRLPNVGGFSVERYAD
                     TIGAALAVGAAAGVAAHAAATGVHQYRERRRQLPVVDADTKGDTHGGH"
     gene            9310..11025
                     /locus_tag="VFU45_07465"
     CDS             9310..11025
                     /locus_tag="VFU45_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEU5358940.1"
                     /translation="MAVTKVVVDPITRIEGHLRIEAQADNGRITGAWATSTQFRGIET
                     IMQGRDPRDAWAFTQRICGVCTVVHAVASCRAVEDALDIRIPPNANLIRNLIHGMQFV
                     QDHVIHFYHLHALDWVDVVSALSADPAATARLAQSLSPWPNNSPTYFGEVKNRLKGFV
                     GSGQLGIFTNGYWGHPAYKLPPEANLMAVAHYLEALDWQRDVIRLHTIFGGKNPHPNF
                     LVGGMASAINLESRATINAARLADIHDLIARAQRFVEQVYWPDLLAIAGFYKEWGAIG
                     GGTGNYLAVGEFPESDIRDLDSLYFPRGIVMNRDLTRVFPYEQQLVKEYINSSWYEYS
                     KGDDAGLHPYEGETTAKYTGPATPWTFLQGEKKYSWMKAPRYDGHPMEVGPLARALVA
                     FASGHKDVREMVKEVMDRLNVGPAALFSTLGRTAARGMETVLLSRRLSHWYDSLVARI
                     QSGDTRTHNGDKWEPSTWPAKAQGYGFLDAPRGALGHWVQIENQKIARYQCVVPSTWN
                     CSPRDQQGQPGPYEAALMDHHPLVKADQPLEILRTIHSFDPCMACGVHVLDATGAEVT
                     RVKVQ"
     gene            11022..11762
                     /gene="cybH"
                     /locus_tag="VFU45_07470"
     CDS             11022..11762
                     /gene="cybH"
                     /locus_tag="VFU45_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HEU5358941.1"
                     /translation="MKAIRKQRGVSLAHPVPPPSGDYHWVYLWEWPIRAMHWAAAGSI
                     LVLFLTGFYIGEPAFLAGGTESRAGFLMGWARFLHFTAAAVFVATGIVRAYWLLAGNQ
                     FERWRALFPVRPRDWVNAWKQVKFYMMIQPEKAPHYLGHNPLQQFSYTGMYAVAALMA
                     VTGFALFGQYEPGGFWYVANQWWVALVGGVQIVRVIHHVAAWAFPIFVPIHIYLAMRA
                     DLLERTGTISSIISGGRFVRADVKYIDE"
     gene            11755..12234
                     /locus_tag="VFU45_07475"
     CDS             11755..12234
                     /locus_tag="VFU45_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HEU5358942.1"
                     /translation="MSSGTVVIGLGNPLMGDDGLGIVVARQLLSRTDLPDGVQVVDGG
                     TWGLNLLPVIEDAERVLLVDAIDMAEAPGTLIRLSREHLPRYLATKISPHQVDLRDVL
                     ALAELRGTLPEDTTAIGLQPQIVALGTALSPLIAGRMEELVSAVLDHLRAREPACTK"
     gene            12222..12476
                     /locus_tag="VFU45_07480"
     CDS             12222..12476
                     /locus_tag="VFU45_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HEU5358943.1"
                     /translation="MHEMSLALEIGRIAELQAGASVRKVITVGVDVGDEAGVEPENLA
                     FCLEAVLATPPFRAKPVIARCRGDVLRVSYLEVDDERPDD"
     gene            12457..13128
                     /gene="hypB"
                     /locus_tag="VFU45_07485"
     CDS             12457..13128
                     /gene="hypB"
                     /locus_tag="VFU45_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEU5358944.1"
                     /translation="MSVRTIEVRERVMAKNDEIARGVRARLHAAGVPAFNLVSSPGAG
                     KTLLLERTLADLGREFRIAVVTGDVQTRNDALRLARHTNRLVQAVVTNGACHLDARQI
                     DSALDAVDLPATDLLFVENVGNLVCPANWDLGEDAKVVLFSVTEGEDKPAKYPKMFQE
                     ARYAVMTKMDLLPHVGFSVEEAVAIATEVNPALTFIYTSAVESEGLEEWYGFLRSQAR
                     RRSAA"
     gene            13125..15395
                     /gene="hypF"
                     /locus_tag="VFU45_07490"
     CDS             13125..15395
                     /gene="hypF"
                     /locus_tag="VFU45_07490"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEU5358945.1"
                     /translation="MTAALDLRVRGVVQGVGFRPFVHRLAVRHGMSGWVRNTSAEVEI
                     WAEGPRDALEAFTHALKSEAPPLARIDQVERLDRDPQGLDGFQIAPSQTDPDRRQPVS
                     PDVALCAACEAELFDPANRRYRYPFITCTDCGPRYTVIEQMPYDRAATTMAAFTQCPV
                     CRAEYEAPGDRRFHSQTNSCPACGPRVWLEGSGGPVSGDAGITAAADALVAGRIVAIR
                     GLGGFHLAVDATAEEAVRRLRVRKHREAKPLAVMVRTLTEARALAVLGDAEAALLASR
                     ERPIVLVSIRPRANLAPSVAPGLTTVGLMLAYTPLHHLLLDRVRRPLVMTSGNQSDEP
                     IAIANEEARRRLDGIADLFLLHDREIAVRYDDSVLRLTGSGPVFLRRGRGYAPLPLAL
                     PIPAPRPLVAVGPHLKNTFTLAAGAEAYVSQHIGDLENLESLEHFRSALAVFERLFRI
                     RPEVVIRDLHPGYLSTRVADELGLRIIPVQHHHAHIAAVLAEHGRREPALGLAFDGTG
                     YGADGRVWGAEFMAADLGGYRRLGHLRYAPLPGGDLAARQPWRSALGYAALDAGGRTL
                     FDPIRPRIPEPERAVAEAQIREGINAPLASSLGRLFDAAAAVLGVCDVSAYEGHAAMR
                     LEHLAGRRVAAALPFPVLEGADGWELDPVPLLAALAERAQRGGDVADLAARFHESIIT
                     GAADLAVRCAGSAGLSVVALGGGCFQNARLLDGCVSRLRSRGFEVLTPRRLSPNDGAV
                     SYGQAAAGAALLAAET"
     gene            15407..15706
                     /locus_tag="VFU45_07495"
     CDS             15407..15706
                     /locus_tag="VFU45_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEU5358946.1"
                     /translation="MCLGIPGRVVERHDEAGLPMGIVDFGGVRRSVCLAYVADEVAVG
                     DYAIVHVGFAISKVNEEEAKRTYAALREMSQLEELDWMREVADRAERAGGAGGGA"
     gene            15703..16806
                     /gene="hypD"
                     /locus_tag="VFU45_07500"
     CDS             15703..16806
                     /gene="hypD"
                     /locus_tag="VFU45_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEU5358947.1"
                     /translation="MKYLNEYRDPEVARGLIEQIKREATRSWVLMEVCGGQTHTIVRQ
                     GLDEVLGGAVEMVHGPGCPVCVTPLEQIDRALALASRPDVIFTSFGDMLRVPGSDCDL
                     HQIRARGGNVQIVYSPLDAVELARRTPDRQVVFFAVGFETTAPANAMAVWRARELGVT
                     NFSILVSHVTVPPAMTAILESPDNRVQAFLAAGHVCTVMGWTEYEPIAARYRVPIVVT
                     GFEPLDILEGMLLAVRQLESGRHEVENQYVRSVRRDGNQPAQDLVSRVFELVDRAWRG
                     IGEIPRSGLGLRAEFAEFDAERRFALEGIRTREPAECRAGDVLRGVLKPMDCPAFGKA
                     CTPEHPLGAPMVSSEGACAAYFNYGRFRASISV"
     gene            16803..17870
                     /gene="hypE"
                     /locus_tag="VFU45_07505"
     CDS             16803..17870
                     /gene="hypE"
                     /locus_tag="VFU45_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEU5358948.1"
                     /translation="MTIDLIAARGLVSCPAPVALDAVVQLGHGSGGKMSAALLRDRFL
                     PHLGNPTLEQLGDAAILPAAGDWLAFSTDTFVVNPLEFPGGNIGSLAAHGTLNDLVMM
                     GARPTALSAGFVLEEGLPLDVLDRVVAALGHSARAAGVMVATGDTKVVERGKADGMYL
                     NTSGVGVVAPGFRPGPNRARAGDVVVISGPVGRHGIAILAARGAIAFEVDVVSDSAYL
                     GPLVDRLRNTLGSDVHALRDPTRGGVASSLNEIASASGVGIVLEEGAIPVPEAVAGAC
                     EMLGFDPLYVANEGVLVALVPGSRADDALMALREHRLGSEATVIGRAVSDHPGLVVMK
                     TGIGGTRIVDMLPGDQLPRIC"
     gene            complement(17875..>18091)
                     /locus_tag="VFU45_07510"
     CDS             complement(17875..>18091)
                     /locus_tag="VFU45_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5358949.1"
                     /translation="PVETLLRELTDWAADLVIVGSHGRNFVMRALLGSVSEELLNQLP
                     TSLLMVPPLRRAGDRVRAATPQLAGAL"
BASE COUNT         2736 a         6026 c         6315 g         3014 t
ORIGIN      
        1 gtctcggggg cacgcaggcg caagccgctg cgggtgcggc gttgaccttg ggtgcgaccc
       61 tcatcctggt gggatttggg agacgggagg cgacgaggtg aaggatcggt acggcatcga
      121 gctctgtgga gtcgggaagg cgtacggcga ccgcgtggtg ctcagcaggc tcgacctgaa
      181 cgtcgcccgg ggggaagtcg tggctctcgt gggcccgaac ggcgcgggga aatcgacgac
      241 gctccgcatc ctgtcgggcg ccattcatcc gtccgtggga gtggcgcgga tcggaggacg
      301 ggatgtcacc ctcgaccctc tgggggcccg ggaatggctg gggtacctgc cgcagcggct
      361 gggggttccc ctgtcgacgg tcgtgcggga cctcgcggcg ctggtgtcca actctcgggc
      421 gctccccgcg gacgccggtg cgcgggcact cgccgaagcg catctcggca aacgtctcga
      481 cgcgacgctc ggacagatgt cgggagggca gcgtcagcgg gtgatgctgg tgttggcgac
      541 gctcggggcg gttgaggcgc tgctcctgga cgaaccgagc atctcgctcg acacagaggg
      601 tgcggaggag gtccgggatg ccatctcggc agcgcggttc gacggcgcgg cgatcctgtt
      661 cgcgtcgcac cacttgagtg acgtagcccg gctggccgat cgcatcgcag tgatggtgga
      721 gggccgagtg gttgcgtgcg gaacggtcga ggagctcgcc cggcgggcgg gcgttgcgtg
      781 ggatccccgc gagcctgagg cgccgatcga gcgcatctac cggaacctga tccagggcgt
      841 tcccgcgccc gttccgatgg ctctcacgct ggtgggcgca tgacgcgcgg ggcgctgatc
      901 gggttcgagt gggcagcggc acgccgcggt cgcgtcgtgc tcttcttcgc catcgggttc
      961 gcgctggcga gcatcggcgt ttccctggcc gggctttcgg cgggaggcat catcgccgtg
     1021 caggggttct cccgcacgtc ggtgagcctg ttgcagctgg tgctatggac ggtgcccttg
     1081 cttgcgctcc tgctgggcgc cattcacggt gcggagtgct acgagctgga gtttctcgcg
     1141 ggcctgcctg cgccacggtc cagtctggtc ttcggccgct gggcggcgtg gaccgcagcc
     1201 ctgggaacgg cgttgatcgg aggattcggg gctgccggcg tggtgatcgg cttgttcgcg
     1261 ggcgctgcgg acgtcctccg gtacctggct ctggtcggga tctccctctt gctcttgtcc
     1321 gcggggctcg ccctcggcct ctggatcggg gtcgcggcgg gaactcgtcc gcgcgccatc
     1381 ggggccgcag tgatcgtctg gttcgtgctc gcgatcggac tcgacctcac cgccatcgca
     1441 gtgctggctg tgttgcctgc gacaggggct acgtggaacc tggcagtgct gctcctggcg
     1501 aacccggtcg acagtgcacg cgcgctggcg ctcggcgtct tcagcgcgga cgcgctggcc
     1561 ggtcccacag gagcatcact ccggcacctg ctgggagggt ggggcgtcct ccccctgacc
     1621 ttgagcctcg cggtctggac agccggtccc ctgtatatcg caggtaagac ttttaacaac
     1681 aaagatttat agcctcaccc gattgtgctg gttatttcgg tagaaacctt catgtcggac
     1741 tgaagcctcc ttcatatccc ctgggcgatt gtcctcacgc acgacaggca gccagcacac
     1801 accaactcgc gtggggagta tctaatgaag gccaggctcg tcgttctcgc agtcgtgatc
     1861 cttggcgcgg tggccatcgg cgcattccgc aatgcccagc cacagcgggt tcagaacgtg
     1921 gatttcgccc agacccccgt ctccaccgat ccgacctatc cggccacagt ggcgcccgca
     1981 ggggatgctc ccgtgaaggc gttccggatc ccgattcggg acgcgaaggt cgaaatcgcg
     2041 gccggcgtca cgtacgccgg ttggaccttc ggcgggaccg ttcccggtcc cgcgattcgg
     2101 gtgcgtcagg gagaccgggt gcgggtgacg gtcgtgaacc aggcctcgat gccacactca
     2161 atcgatttcc acgcggcgcg cgtgcccatg aacgtcgact tccgcatgat catgccccgc
     2221 gactcgattt ccttcgagtt cgtggcgcgc gatcccggcg cctctctggt gcactgcggc
     2281 acgcccccgg tgctgctgca cctgatgcag ggcatgtacc ttccgatcat cgtcgacccg
     2341 aagggcggct ggcccggcaa ggcggacaag gagttcgtgc tggtgcagag cgagttctat
     2401 gtccagggcg gggacagcgc gcagccgggg cagcccgact ggcagcacgc gctcgaccgg
     2461 caggccagct acgtcgtttt caacgggaag gcctttcagt accagagcac gcccctccaa
     2521 gtcaacgtcg gtgaccgcgt ccggttcttc gtcgtgaacg ccggtccgag cttcacgagc
     2581 gacttccaca tcgtgggcgc gatcttcgac cgggtgtatc cggatgggga tccgggccac
     2641 gccctcgccg gcgtccagac ttggggcatt cccgcgggct ccggagcggt attcgagacg
     2701 catttcgcgg ccgacggcag cggagccggg acttacgcgt tcgtcaccca cgcgttcgcc
     2761 gatgcggcca aaggcgcggt aggattgatc cacgtcggcg acggccccgc ggtcgcgatg
     2821 gcacactgag tgccggtcct cacgcgctgg tacgtcaaga cggcgattct gtatctggta
     2881 gttgcgctgc tgctgggagc agttctcgcg gggaacggcg tggtgccgct ccccgcgttt
     2941 ctgctcgtgg ccgggccggc gcagattcat ctcttcgtcg tgggttggct gacgcaactc
     3001 atcttcggca tcgcgtactg gatgtttccc aaagccacgg cccagcagcc gcgaggcaac
     3061 gacgcgctgg ccgtgctgac ctatgtctgc ctcaacttcg ggctcctggc tcgggtcatc
     3121 ggagagcctt ggcacgcggt ggcgccgggt gtcctggcgg gtgggctcct ggtggcgtcc
     3181 ggcgtgctcc agtggctcgg gggtgttgga ttcgcggtgc tcgcatggcc gcgggtgcgg
     3241 gggcgctgac gccgtgccgc gggtgagcat ctggatggtt cggtcggcgc tggtggcact
     3301 cgtgctggga ttctcgatcg gcgccatcat gctgatcgga aagggcaccg gcgcgttcgg
     3361 tggattgtcc gccggcattc cgattcacgt ggagttggtg gtgctcggct ggatggtgca
     3421 gctcgcgatg ggtgtggccc tctggatctt gccccgcttc ggcgcccgcg gcccggcccg
     3481 cggcgagacc tggggctggg ccgcctgggc cctctggaac agcggcatcg tattcgtcat
     3541 cgtcggcgcg gctggtgctc ccgcggtacg gctcatcggc cggttgatcg agctgggcgc
     3601 ggccatcgca ttcggtgcgg ccgtctggag ccgcgtgcgc gcgtccggga tctccgcgat
     3661 gtgatcggcc gtccggacta gcgggcgcgc acccggcccc cttcgaccac ctgatcgccg
     3721 gggtcacgga tcacggtcgc gccggccttc aacccaccgg tcacctcgac atcgaaaccg
     3781 gagcggtgcc cgacttccac cgaacgctga tgaacccggc cattctcgac ctggaacacc
     3841 gcccagctgg ggccgaggcg gaacagggcg ctggccggaa cctgcaacac cccgggggac
     3901 cgccagacca cgatctgcac atcgacccgg aaccgatccc ccagccccgc cgggacggta
     3961 ttcagaatcc ccaccacgtt cactctctgt tcctcgacgc cgagcgccga caccttggtg
     4021 aatccggacg gctcgaccct gatcacgtgg cccatcaggg cgctgtcccc tccccagccc
     4081 tccacccgca tttcgtctcc tgggtggacc cgcacagcgt cggacgagag cagatcggtg
     4141 acgatctcga tgtttcgggg atcgccgatt tccagcagcg gcgtgccggg cgcgacgacc
     4201 cgctcgctgg ggtccggcac gcgcagcagt tggccccgaa ccggcgagcg cagggccacc
     4261 gcctcccggg ctccaccgag gagggctgac cgggccactt cgacatcatg cgccgccgcc
     4321 tgtgcgcgga attgcgcgga ttccagctcg cgctgccggt tcgtttcgtc cagctcggcc
     4381 cgttcccgtt cctccggcgc gatcagatgc tgggcggcga gctcctgcgc gcgatcgcac
     4441 gaccgtcggg cttggtcgta cgcggcccgg gcttgcccgg ccaccgcgtc tgccgcgcgc
     4501 tgggcgtctt ccgcttcggc cactcgtgcg gccgcctgct cgtgcgcccg cgcgtcgagg
     4561 ggggatggga agatccacgc gacgatggtg ccgggctgca ccgggtctcc ctcccgggcc
     4621 tggatgcggg cgacccggcc ggcgacgggc gcggtgatgg tgtagcggtc gaccactcgg
     4681 gtctgccctt cttcgtcgat ggtgacttcg agaggacccc gggtgacggt cgtcagatcg
     4741 acggcggcgg gcgccggacg gaacaggaag aaggctccgc cgaggagacc ggcgccggct
     4801 gccagaacaa ggatgcgctt ccgcgtcgtg gtcatgggat cattcccgcg tcttgagaac
     4861 ttcgaccagg ttgaggcggt tcaggcggcg gcggatcagc agtgccgaaa cggccgctgc
     4921 ggcgagcacc accagcgcgg cgttgaggta ggtagccgcc gacaccacca ggggcaggcg
     4981 gaacacctcc cactggtaca gatccgagaa cagcgcgcag aagccatagc ctatcgccaa
     5041 ccccgcgccc gttccgagaa gcgtgaggac tgcctgctca ctgaggagca tgcgggcgac
     5101 ctcccaccgc gtgaacccga gcacccggag gctcgccagc tcccgagccc gctcggagag
     5161 agacaggcgg gcggcgttat agacggttgc gaccgccaac gccgccgcga accccacgaa
     5221 gaccaccgtg acgatgccga tgctctgcgc gagcgtggtt tcgaagctgc cgagcgacga
     5281 ccggcggtcg gcgacgccgg ctaccgccgg gagggccttg aggcgggcgt tgagggccgc
     5341 gaggtcgcga gcgtcggtgc gcagaaagac cccggacaca gtctggtctt cccccaacaa
     5401 ggaagcgagc gaccgccggt ccatgtagcc ggtgaggccg acgagctcgt ccacgaaccc
     5461 ctcgaccacg acgtctctcc gcggacggct gccctccagc acctcgacgc tgaccgagtc
     5521 cccggcgcgc acacccagga tgtcgcccag cttcgcggtg agcacgagac cgccgggcgc
     5581 gagacgcacg ggtctccagc cggtcccgac cagtcggtgc atctcactct cgggctcgag
     5641 gccgagcaca ggtattcggc gggatcggtg cccgttggac acacgggccg gcacggtgcg
     5701 gaatccctcc gcgctgagca cgccagggag ggcgcgggcc gcgaacaccg cgtctggctg
     5761 ccggggccgg ttgaaggcga cggtgaattc ctcgcgctga acccggttga attgcacatc
     5821 cgcgatggtc cacatggcgt ccacgaagta cctgccgacg atcaggatgg ccgccgcgaa
     5881 cgcgatcccc gtcatggaca gcacggagcg cagggggcgt cgttccagat cccgcgccat
     5941 gatccgggtc gggggtgaga gcaggcggtg caggcccagg cgctccagga gccccggacg
     6001 gaaccggggc ggcgcggccg cctgcatggc ggtcgccggg ggaagctgaa cgacccgccg
     6061 taccgcgccg gtcgcccccg cgagtgcggc caggagcgtc accccgacgg cgagggcgac
     6121 cgccgcccag tccagccgga aaacgaagac ggggaaccgg tagaaccgcg agtaattgag
     6181 attgacgcgc tcggcgaacc agagacccag cccgatgccc agggccgcgc ccagcgcgag
     6241 agcgacgagc gcgagtttga ggtagtgcag ggcgacggtc cggttctcat acccgaacgc
     6301 cttcagcacg ccgatctgct cccgctcgat ccccaccagc cgggtcagca cgatgttcag
     6361 gagaaaagcg gcgacgccga ggaagatggc ggggaccaga gtaccggaga cgcggttctg
     6421 gttgagctcg tcgttcagga atcgcgccga gacctggtcc gcgcgtccgt acgcgtccag
     6481 ccctccgtac ggcgcgagga tccggttgac gcgcgtgatg acatcggcct ccgatgcacc
     6541 cggggcgagc cggatggaga cgtcattgaa cgcgccggtc atgtcgaacg ccgcggccag
     6601 gacggcgcgg ttcatccaca ggacgccgaa gcgccggctg tcgggaaata gctgagcctc
     6661 tccgatttcg tacacatact cgggtgaaat cccgacgccc acgacgcgca gccgctgcca
     6721 gcgtccgttg atcaccgcac cgagcgtcgc gcccaactcg aggtggttcg cggtggcgaa
     6781 ggcctcgctc acgatcgctt cgtccgggcg gtcggcctcg atccagcggc cgcgccgcaa
     6841 gtgcacgtca ttgagccgcg gccggcccgc cgcggggacg ctgatgagtc gtcccgaggc
     6901 gggttccgcg agtcccggga catccagcgt cacggcggtc acgacgcggg tgtcgatcgc
     6961 cgacacgccc ggaaccgccg ccacccgtcc cgccaccgct tcgggtgccc gcttgaggtg
     7021 cgcgaacaca tcggcgaacc ggtagtcgcg gtaatacgcc gctcgtgacg cgccgaggga
     7081 ggtgtatgag gttcgcgtcg tgacgaccac cgccacaccg cacgccacca ccaggctgat
     7141 ggccgcaacc tggccgcgga ggtgccagag gtcgcgcacc agcttgcgat caagcaggga
     7201 caggcgcgtc accacgacaa ctccgccgga cggctgcgac gctggttcac gctcagctcc
     7261 gtgatggttc cgctccgcag acggatgacg cggtcggcca tggcggcgat actggcgttg
     7321 tgggtgatga ccgcggtggt ggtgcccagc tcccggttca cctggtcgag cacctcgagg
     7381 acgatcttgc cggtctcata gtcgagggcg ccggtcggct cgtcgcacag cagcgcgtcg
     7441 ggccgcttgg cgatcgcccg ggcgatcgcc acgcgttgct gttcgcctcc ggacagttga
     7501 gcggggaaat ggtcgagtcg ttccccgagc cccacgagcc gcagggcctc ggccggatcc
     7561 atggggttcg tcgcgatggc ggtcaccagc gcgacgtttt cccgagccgt gagcgtcgga
     7621 atcaggttgt agaactggaa gacgaaaccc acgtgttccc ggcggaatcg ggtcagctcg
     7681 cggtccgagg cgcccgtgag gtcgtggtcg aggaaatgga cgcgcccacc ggtcggggca
     7741 tcgagacctc ccaggatatt gaggagggta gacttgccgc ttccggacgg cccgaggatg
     7801 accacgaatt ccccgcggta gagctcgaga tccacggcac ggagggccgg gacttcgacg
     7861 tcacccaacc ggtacatctt cgagaggccg agcgcccgga agaccacatc ccgccccgcg
     7921 agggccgttg tccccagctg gggaagcgcc gttgccgccg tcatgatctc aattgcgcag
     7981 gccgaccgcg aaggacctct gagaatcctt cgaaggtccc gtgcgccaga tcgtgaaggt
     8041 ctcttcatag tttcttcgac tttcttgctt gccccgatac gcattctccc tgcacgattg
     8101 aattgttctg atggacgacc ccggtcccat ccaccgatgc cagtcactcg ggcgactgca
     8161 gcggagcgtg ccccatgttc tttccgcaaa cccatccatg gactgaaggc cagtccctca
     8221 ccgactatct cgagcagcgc ggcgtcaacc ggcgcgattt cctgacttgg tgcgcggaaa
     8281 tggcggccgt gctcggcctc tcgagcgtgg tgaccccgcg ggtcgcggaa gccatggtgg
     8341 cgcaaaagcg tccctccgtc atctggctgc aactgcagga atgcacgggc tgcgtcgaaa
     8401 gcgtgctccg caccgaggcc ccgaccatcg gggatctgat cctcgatgtc gtctctctcg
     8461 attaccagca cacgctcatg gcgggcgcgg ggaaggccgt cgaggacgcc ctgcggaccg
     8521 cggaagaaaa gaaccggggt cagtatctgc tgatcgtgac gggctccatt ccgctcaacg
     8581 acaacgggat ctactgcacc atcggtgggc gcacggccaa ggagatcctg ctcgaggccg
     8641 gacgaggggc gaaggcggtc gtggcgatcg gcgcctgcgc acactggggc agtgtacagg
     8701 cggcgaagcc caacccgacg ggcgcggtgg gggtcgccga cgtcctgaag gaccggccca
     8761 tcgtgaacat cgccgggtgt cctcccatcg ccgacgtcgt gacggcgacc atcgtccact
     8821 atctgacctt cggccgcctg ccggcaaccg attacgatgg gcggccgctc ttcgcctacg
     8881 gcgcccgtat ccatgatcag tgcagccgcc gtgcgaattt cgacgccggc cagttcgtcg
     8941 agaaattcga cgatgacgcg gcccggcgcg gatggtgcct ctatcacgtc ggatgcaaag
     9001 ggccggagac gttctcaccg tgcccgattt tcctctggaa tgacaagacc agctggccca
     9061 tcggcgccgg gcacccgtgc ctcggctgca ccgagcgcca cttctgggat accatgaccc
     9121 cgttttacgg gcgcctgccc aacgtcggcg gattttcggt ggagcgatac gcggacacga
     9181 tcggtgccgc gctcgcggtc ggggccgcgg ctggcgtcgc ggcacacgca gcggccaccg
     9241 gagtgcacca gtatcgcgaa cgccgccgcc agctgccggt agtcgatgcg gacacgaagg
     9301 gagacacgca tggcggtcac taaagtcgtc gtcgatccga tcaccaggat cgagggccat
     9361 ctccgcatcg aggcccaggc ggacaatgga cggatcaccg gcgcctgggc gacgtcgacg
     9421 cagttccgcg gcatcgagac catcatgcag gggcgggacc cgcgggatgc ctgggccttt
     9481 acccagagga tctgcggcgt ctgcacggtc gtccacgcgg tcgcctcctg ccgtgccgtc
     9541 gaagatgccc tcgatattcg gattcccccc aacgccaacc tgatccgcaa cctgattcac
     9601 gggatgcaat tcgtgcagga tcacgtcatc cacttctacc acctgcacgc gctcgactgg
     9661 gtggacgtgg tcagtgcgct gtcggctgat ccagcggcga cggcgcggct cgcgcagtcc
     9721 ttgtctccct ggccgaacaa ctctcccacg tacttcgggg aggtgaagaa ccgtctcaag
     9781 ggattcgtgg gatccggaca gctcgggatc ttcaccaacg ggtactgggg ccacccggcc
     9841 tacaagctgc cgcccgaggc caacctgatg gcggtggcgc attacctcga agcgctcgac
     9901 tggcagcggg acgtgatccg gctgcacacc atcttcggcg gcaagaaccc gcaccccaac
     9961 ttcctggttg gcggcatggc ctcggcgatc aatctcgaga gccgcgccac gatcaacgcc
    10021 gcccgtctgg cggacattca cgacctcatc gcccgggccc agcggttcgt ggagcaggtc
    10081 tactggcccg atttgctggc catcgccggc ttctacaagg agtggggagc catcggcggg
    10141 gggaccggca actatctggc ggtgggggag ttcccggaga gcgacatccg ggacctcgac
    10201 tcgctctact tcccccgcgg gatcgtgatg aaccgcgacc tcacgagggt ctttccgtac
    10261 gagcagcagc tggtgaagga gtacatcaac agctcctggt acgagtactc gaagggggat
    10321 gacgccgggc tccatcccta cgagggcgag accaccgcga agtacaccgg gcccgccacg
    10381 ccctggacct tcctgcaggg ggagaagaag tactcctgga tgaaggcgcc gcggtacgac
    10441 ggccacccga tggaagtcgg gccgctggcg cgtgccctgg tggcgttcgc ctcaggccac
    10501 aaggacgtgc gagagatggt gaaggaagtg atggaccgcc tgaacgtggg gcccgcggcg
    10561 ctgttctcca cgctggggcg gacggcggcg cgcgggatgg agaccgtgct gctctcccgc
    10621 cggttgagcc actggtatga ctccctcgtc gcgcgcatcc agagcggaga cacgcgcacc
    10681 cacaacggcg acaagtggga gccgtcgacg tggccggcga aggcccaagg gtatggattc
    10741 ctcgacgcgc cgcgtggcgc gctggggcat tgggtccaga tcgagaacca gaagatcgcg
    10801 cgctatcagt gcgtggtgcc gagtacctgg aattgctcgc cgcgcgacca gcagggtcag
    10861 cccggcccct acgaagcggc gctgatggat caccacccgc tggtgaaggc ggatcagccg
    10921 ctcgagatcc tccgcaccat ccactcgttc gatccgtgca tggcctgcgg agttcacgtc
    10981 ctcgatgcca ccggcgcgga agtgacgcga gtcaaggtgc aatgaaagcg atccggaagc
    11041 agcgcggcgt ctccctggcc cacccggtgc cgcccccctc cggggactac cattgggtgt
    11101 acctgtggga gtggcccatc cgcgccatgc attgggcggc cgcggggtcc atcctggtgc
    11161 tgttcctcac cgggttctac atcggggagc cggcgttcct ggccggcggc accgaatccc
    11221 gcgccggttt tctgatgggg tgggcgcggt ttctgcactt caccgccgcc gccgtgttcg
    11281 tggcgaccgg catcgtgcgc gcctactggc tgctggcggg gaatcagttc gagcgctggc
    11341 gcgcgctctt cccggtccgc ccccgcgact gggtcaacgc gtggaagcag gtcaagttct
    11401 acatgatgat tcagcccgag aaagccccgc actacctcgg gcacaatcca ctccagcaat
    11461 tctcctacac ggggatgtac gccgtcgccg cgctgatggc ggtgaccggc ttcgcgctct
    11521 tcggccagta cgagccgggc ggcttctggt acgtcgcgaa ccagtggtgg gtggcgctcg
    11581 tcggcggcgt tcagatcgtc cgggtgattc atcacgtggc ggcgtgggcc tttcccatct
    11641 tcgtcccgat tcacatctac ctggcgatgc gcgcggatct cctggagcgg accggcacca
    11701 tctcctcgat catttcgggt ggccgcttcg tccgcgccga cgtgaagtac atcgatgagt
    11761 agcggcaccg tcgtcatcgg cttggggaac cccctgatgg gagatgacgg gctgggcatc
    11821 gtcgtggccc ggcagctcct gtcccgcacg gaccttcccg atggagtcca ggtggtggac
    11881 ggggggacat gggggctcaa cctcctcccg gtcatcgagg acgccgagcg cgtactcctg
    11941 gtcgacgcca tcgacatggc tgaggcgcct ggaaccctca tccggttgtc ccgggagcac
    12001 ctccccagat acctggccac caagatctct ccccatcagg tcgacctccg ggacgtgctc
    12061 gcgctcgccg agctgcgggg gacattgccg gaagacacca cggcgattgg gctccagccg
    12121 cagatcgtag ccttgggcac ggccctcagt cctctgatcg ccggacgcat ggaggagctg
    12181 gtgtccgccg tgctcgacca cctgcgcgcc cgtgagcccg catgcacgaa atgagtctcg
    12241 cgctggagat cggacggatc gcggaattgc aggcaggggc gtccgtccgg aaggtgatca
    12301 ccgtcggggt ggacgtcgga gatgaggcgg gagtggagcc cgagaacctg gctttttgtc
    12361 tcgaagcggt cctcgcgacg cccccgttcc gcgcgaagcc cgtgatcgcc cggtgccggg
    12421 gagatgttct gcgggtcagc tatctggagg tggatgatga gcgtccggac gattgaagtc
    12481 cgtgagcggg tgatggcgaa gaacgacgag atcgcgcggg gtgtgcgcgc ccgcctgcac
    12541 gcggcgggcg tccccgcgtt caacctcgtc tcctccccgg gcgcggggaa gacgctgctc
    12601 ctggaacgga ccctcgccga cctcgggcgg gagttccgca tcgcggtggt cacgggagac
    12661 gtgcagaccc ggaacgacgc gctccgcctg gcgcgtcaca ccaaccgcct ggtgcaggcg
    12721 gtcgtgacca acggtgcctg tcacctggat gcccggcaga tcgactcggc gctcgacgcc
    12781 gtcgaccttc ccgccaccga tctcctcttc gtcgagaatg tcgggaacct ggtgtgtccg
    12841 gcgaactggg atctcgggga ggacgcgaag gtggtcctct tctccgtcac cgagggtgag
    12901 gacaagccgg ccaagtatcc gaagatgttc caggaggcgc ggtacgcggt catgaccaag
    12961 atggatctgc tgccccatgt ggggttcagc gttgaagagg cggtcgcgat tgccaccgag
    13021 gtgaatcccg ccctcacctt catctatacc tccgcggtgg agtccgaagg tctggaggag
    13081 tggtacggct tcctgcgaag ccaggcccgg cggcgcagcg cggcatgacg gccgcgctcg
    13141 atctccgcgt ccggggggtg gtgcagggcg tggggttccg tccgttcgtc caccggctcg
    13201 ctgtccgtca cggcatgagc ggatgggtcc gcaacacctc cgcggaggtt gagatctggg
    13261 cggagggtcc gcgcgacgcg ctcgaggcgt tcacgcacgc tctcaagtcc gaggccccgc
    13321 cgctcgcccg catcgatcag gtggagcggt tggaccgtga tcctcagggg ctcgacgggt
    13381 tccagatcgc cccgagtcag accgatcccg atcgccgtca gccggtgtcc cccgacgtgg
    13441 ccctctgcgc ggcctgtgaa gccgagctct tcgatcccgc caatcggcgt taccggtatc
    13501 cgttcatcac gtgtaccgat tgcggcccac ggtacaccgt catcgagcag atgccgtacg
    13561 accgggccgc gaccacgatg gcggccttca cgcagtgtcc ggtctgtcgt gccgagtacg
    13621 aggcccccgg ggaccgccgg ttccactctc aaaccaacag ctgccccgcc tgcggtccgc
    13681 gggtctggtt ggaagggagc gggggaccgg tttcgggtga tgcgggcata acggcagccg
    13741 ccgatgcgct cgtcgccggc cgcatcgtcg cgatccgggg actgggaggg ttccacctgg
    13801 cggtggacgc gacggcggag gaagcggtgc gccgcctgcg ggtccgcaaa caccgcgagg
    13861 cgaaacccct ggccgtgatg gtccgcacgt tgaccgaggc ccgcgccctc gcggtgctgg
    13921 gcgatgccga ggcggccctg ctcgcatcgc gcgaacgccc catcgtcctg gtgtcgatac
    13981 ggccgcgcgc gaacctggcg ccgtcggtcg cacccggcct cacgaccgtc ggtctcatgc
    14041 tcgcctacac gcccctccat cacctcctgc tggatcgcgt ccgccgcccc ctggtcatga
    14101 cctccgggaa ccaaagcgac gagccgatcg cgatcgccaa cgaggaggct cgccggcggc
    14161 tcgacggcat cgccgatctc ttcctgctgc acgaccgcga gatcgccgtc cgctacgacg
    14221 attcggtgct gcggctcacc ggctcggggc ctgtgtttct gcggcggggc cggggttacg
    14281 caccccttcc ccttgccctc ccaattccgg ccccgcgtcc cctggtcgcg gtggggcccc
    14341 atctcaagaa cacgttcacg ctcgcggccg gcgccgaggc gtacgtcagc cagcacatcg
    14401 gcgatctcga gaacctcgaa tcactggagc acttccggtc cgccctcgcg gtctttgaac
    14461 ggctcttccg gatccggcct gaggtcgtca tccgggatct gcatccgggg tacctctcga
    14521 ctcgagtggc ggacgagctc ggcctgcgga tcatcccggt ccagcatcat cacgcgcaca
    14581 tcgccgcggt gctggccgag catgggcgcc gggagccggc gctgggactg gcgttcgacg
    14641 gaaccgggta cggtgcggac ggacgggtct ggggagccga attcatggcg gccgatctgg
    14701 gcggctaccg gcgcctcggg catctgcggt acgcgccact gccgggcggc gatctcgccg
    14761 cccggcagcc ctggcgctcc gccctgggct acgccgcgct ggacgcgggc ggccggacgc
    14821 tgttcgaccc gatccgcccg cgcattcccg agcccgagcg cgcggtcgcc gaggcacaga
    14881 ttcgcgaggg gatcaatgcg ccgctcgcgt catctctggg acggctgttc gacgcggcgg
    14941 ccgccgtgct cggggtgtgt gacgtatcgg cgtacgaagg gcacgcggcg atgcgcctgg
    15001 agcatctcgc cggccggcga gtggccgcgg cgttgccgtt tccggtcctc gagggggccg
    15061 acggttggga gctcgatccg gtgccgctcc tcgccgctct cgcggagcgc gcgcagcggg
    15121 ggggcgacgt cgcggacctc gcggcccgct tccacgaatc gatcatcacc ggagccgcgg
    15181 accttgcggt gcggtgcgcg gggagcgcgg gactctcggt ggtggcgctt ggcggcggat
    15241 gctttcagaa cgcccgcctg ctggatggat gcgtctcccg gttgcggagc cgcgggttcg
    15301 aggtgctgac cccgcgccgg ctctccccga acgacggcgc ggtgagctac gggcaggcgg
    15361 cggccggggc ggccctgctt gccgcggaga cataaaggag aaggccatgt gcttgggcat
    15421 cccgggaaga gttgtcgaac gccacgatga agccggtctc ccgatgggga tcgtggactt
    15481 cgggggcgtg cgccgttcgg tgtgtctggc gtacgtcgcc gacgaggtcg ccgtcggcga
    15541 ctacgcgatc gtgcacgtgg gatttgcgat cagcaaagtg aatgaagagg aagcgaaacg
    15601 gacctatgcg gcgctgcggg aaatgagcca gctcgaagag ctggactgga tgcgggaagt
    15661 cgcggaccgc gctgagcggg ccggtggggc ggggggcggc gcatgaagta cctgaacgag
    15721 tatcgcgacc ccgaggtcgc ccgcgggctg atcgagcaga tcaagcggga ggccacccga
    15781 tcctgggtgc tcatggaggt ctgcggcggg cagacccaca cgatcgtccg tcagggtctg
    15841 gacgaggtgc tcggcggggc ggtcgagatg gtgcatggcc ccggctgtcc cgtatgcgtg
    15901 acgccgctcg agcagatcga ccgcgcgctg gcgctggcat cccggcccga cgtgatcttc
    15961 acctcgttcg gggacatgct gcgtgttccc ggaagcgatt gtgacctgca tcagatccgg
    16021 gcccggggag ggaacgtcca gatcgtgtac tccccgctgg acgccgtgga gctggcccgc
    16081 cggactcccg accgccaggt cgtgttcttc gccgtgggat tcgagacgac ggcgccggcg
    16141 aatgccatgg cggtgtggcg ggcgcgcgag ttgggggtga cgaacttcag catcctcgtc
    16201 tcccacgtca ccgttccccc ggccatgacg gcgattctgg aatcgccaga caaccgggtg
    16261 caggcgttcc tggcggcggg acatgtctgc acggtgatgg ggtggaccga gtacgaaccc
    16321 atcgccgccc gctaccgggt tccgatcgtc gtgaccgggt tcgaaccgct cgacatcctg
    16381 gaagggatgc tgttggcggt ccggcagctc gagtccgggc ggcacgaagt cgaaaaccag
    16441 tatgtgcgct cggtgcggcg ggacgggaat cagccggctc aggatctggt gagccgcgtc
    16501 ttcgagctcg tggaccgggc ctggcggggc attggagaga ttccccggtc cggactggga
    16561 ttgcgggccg agttcgccga gttcgacgcc gaacggcgct ttgccctgga gggcatccgg
    16621 actcgtgagc ccgccgaatg ccgcgccggg gacgtgctgc gcggggttct gaagccgatg
    16681 gattgtcccg cgttcggcaa ggcctgcacc cccgagcacc cgctgggtgc gcccatggtg
    16741 tcgtccgagg gcgcctgcgc cgcttacttc aactacgggc gcttccgcgc gtccatctcc
    16801 gtatgaccat cgatctcatc gccgcccgcg gactcgtctc ctgtcccgcc cccgttgcat
    16861 tggacgccgt ggtgcaactc ggtcacgggt caggcggcaa gatgtcggcc gccctgctcc
    16921 gtgaccggtt cctgccccat ctcggaaatc cgacgctcga gcagctcggc gacgcggcga
    16981 tcctgccggc cgcgggcgac tggctggcgt tttcgaccga cacgttcgtc gtcaatcctc
    17041 tggagtttcc cggtggcaac atcggctcgc tcgcagcgca cgggaccctc aacgatctcg
    17101 tcatgatggg tgcccggccg accgccctgt cggcgggctt cgtcctcgag gagggcttgc
    17161 cgctggatgt gctcgaccgc gtagtcgccg ccctcgggca ctccgcgcgc gccgccggcg
    17221 tcatggtcgc aaccggtgat acgaaggtgg tggagcgagg gaaggcggac gggatgtacc
    17281 tcaataccag cggagtcggc gtggtggcgc caggattccg gccggggccc aaccgggccc
    17341 gtgccggtga tgtggtcgtc atttcaggtc ccgtaggccg tcacggtatc gccattctcg
    17401 cggcccgcgg ggccatcgcg ttcgaggtcg acgtggtgag tgactcggcc tacctgggcc
    17461 ccttggtcga ccggctccgg aacacgctcg ggtccgatgt gcacgccctg cgtgatccga
    17521 cccgcggtgg cgtggccagc agtctgaatg aaatcgcgtc ggcgtcaggc gttggcatcg
    17581 ttctcgagga gggggcgata cccgtacccg aggcggtggc gggagcttgt gaaatgctcg
    17641 ggttcgaccc gctatacgtg gcgaatgagg gcgtactggt agccctggtc cccggttccc
    17701 gcgctgatga tgcacttatg gccttgcggg agcatcgcct tgggtccgag gcgaccgtga
    17761 tcgggcgtgc ggtttccgat caccccggtc ttgtggtcat gaagaccggg atcggcggta
    17821 cccggatcgt ggacatgctg cccggggatc agctgccccg gatctgctga gcccctagag
    17881 cgctccagcc agttgggggg tggcagcgcg gacccgatcg ccggcccggc ggagtggcgg
    17941 gaccatcagc agggacgtgg ggagctggtt caggagctcc tcgctcacgc tccccagcaa
    18001 tgcccgcatg acgaagttcc ggccatgcga tccgacgatg accagatccg ccgcccagtc
    18061 ggtcaactcg cggagcaggg tctccaccgg g
//
LOCUS       DASUAP010000281         6251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_198609, whole genome shotgun sequence.
ACCESSION   DASUAP010000281 DASUAP010000000
VERSION     DASUAP010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198609"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFU45_07515"
     CDS             <1..682
                     /locus_tag="VFU45_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dehypoxanthine futalosine cyclase"
                     /protein_id="HEU5358950.1"
                     /translation="HIHGFSPSEVVFFSERFQLPVPEVIRQLRAAGLDSIPGGGGEIL
                     VDAVRARVARKKAQTDAWLEVQEEAHRQGMKTSVTMMYGLGESNADRVEHLFRVRAVQ
                     RRTGGFTAFICWPLQPEGTPMFTGQAKTDAVTYLRTLAMARIVLDNVPNLQSSWVTMG
                     HKIGQVALRFGANDYGSLMMEENVVSAAGTTYRTTLTEIERLITDAGYAPRRRRQDYS
                     IIEATAAA"
     gene            679..1158
                     /gene="ispF"
                     /locus_tag="VFU45_07520"
     CDS             679..1158
                     /gene="ispF"
                     /locus_tag="VFU45_07520"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEU5358951.1"
                     /translation="MSRTGIGYDSHRFAAGRPLILGGITIPSDRGLTGHSDADAIAHA
                     LTDALLGAAAAGDIGRLFPDTDPQWKGADSMTLLAAAHAAAQAKGWRFVQGDITIICE
                     RPKLAPHLPAIAERLAAVLGVTPDAVGVKAKTNEGMGWIGRAEGIAVMAVATVEPVR"
     gene            1242..1844
                     /locus_tag="VFU45_07525"
     CDS             1242..1844
                     /locus_tag="VFU45_07525"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEU5358952.1"
                     /translation="MFPPVPADTAVALGAFLSQQGVTTPWTVFTVTILANTASAALVY
                     GVARRFGPRFAATGLGRRLLPADALATLERKYLRWGLPAIFLSRMIPGIRAIVPPFTG
                     IIGLSPRRALLPIAAASAVWYGFVTVVGVVLGSEWDTMVRLLGQVNRALAVVGAVAAL
                     AIATGLLVRSRARRRHHQQPVTGELVDAVLQKALHKGPAA"
     gene            1841..2749
                     /gene="xerD"
                     /locus_tag="VFU45_07530"
     CDS             1841..2749
                     /gene="xerD"
                     /locus_tag="VFU45_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU5358953.1"
                     /translation="MTREELERAFLLEGFRDHLSLEAGASPNTVESYLRDLRHLVEFA
                     GAAGLTGPDRLTARTLRDFVFGLKDLGLGPATIRRHVSAVRTYFRFAVGEGVVAADPS
                     DRLEAPRRGRKLPDTLSVADVSAMLEAPPLDDPLAWRDRALLELAYGAGLRVSELCGL
                     TPADLWLDDQVVRVFGKGSKERIVPLGRTVIGAVAVYLRQLRPELDTGASGNRLLLNF
                     RGRPLSRVGAWGIVKKAAKRAGIRKRVTPHTLRHSFATHLLEGGADLRAVQEMLGHAD
                     LSTTQIYTHVDREYLRSVHRQFHPRG"
     gene            complement(2757..4058)
                     /locus_tag="VFU45_07535"
     CDS             complement(2757..4058)
                     /locus_tag="VFU45_07535"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU5358954.1"
                     /translation="MYPASPAHSSEYQVPGRGGARPLTADEEKAARGFVLRLGRALHN
                     VGYAAHRVETLMERASDRLGLTGQFFATPTSIFAGFGAEDEQRTFLMRVGAADDDLGK
                     LADLDEVAGQVLRGAIPPLAGSARIDEIVASPPRYNDLVTVVAFGLLSACGARFLGGG
                     SVEVEVSAVIGLVVGLLNALAIRVPALGRVFPVAAAFVASMLAVVSGKFVHGITVYTV
                     TLSGLLVLIPGFTLTTAMTELATRHLIAGVARFSGAMMLFISIAFGVALGTKTATYIT
                     GKLMPASAVPLPGWTIVAALLLAPLAFTILLRANPRDTGWILLTGVVTYYGGILGASI
                     LGPEVGAFVGALSAGLASNVYAWLTDRPTSVTLVPSVLLLVPGSVGFRGLASLLDRQT
                     ILGVETAFQMVLIASALVAGLLVSFVLGPQRRRYRRDIFAS"
     gene            4411..4659
                     /locus_tag="VFU45_07540"
     CDS             4411..4659
                     /locus_tag="VFU45_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358955.1"
                     /translation="MRVNAMVLSTRIPSVARVAVQPAGPAGQTRVATRANTGWSVSIS
                     GSAAKGDSVQVRSPASGGMVTPVETRQGGLMIVTLAER"
     gene            4702..5421
                     /locus_tag="VFU45_07545"
     CDS             4702..5421
                     /locus_tag="VFU45_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358956.1"
                     /translation="MQHPENHDGEGDTVRNYWFKIAGGALAIFAVGLAGVYAVRGIHR
                     RVHDLANGTGDITIPLLGIVPLSIGNVRLGALNKIEIHRDAPKHVDGFTVLARLDDSV
                     DADRFADCFFTLQDPTRINDKTTFTCLDSVPAGMRQFGDLRLIDSSGDAVSERPLILA
                     ESDIADLQNVHGGGTPNADSIRASVESIGAAARKMGDSIRREMRARYGKGSTGTVYQP
                     TRPDPPMPQKPPAQPAPNARP"
     gene            5544..6146
                     /locus_tag="VFU45_07550"
     CDS             5544..6146
                     /locus_tag="VFU45_07550"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU5358957.1"
                     /translation="MLLVIDNYDSFTWNLVQYIGELGVEPVVYRNDRLSVDEALALAP
                     EAILVSPGPCTPREAGIAVSLIRRAAGRIPVLGVCLGHQAIGEAFGGQVVRADRLMHG
                     KTAAVRHDGSSLFDGVPSPVTVMRYHSLVVAPDRLPPELEVTAWSADRGREDEIMGLR
                     HRQHPIWGVQFHPESVGTEHGRRILANFLALAGIPAGSGR"
BASE COUNT          963 a         2211 c         2115 g          962 t
ORIGIN      
        1 ccacatccac gggttctcgc cgtcggaggt cgtgttcttc agcgagcgat ttcagctccc
       61 cgtgccggag gtgatccggc agctccgcgc ggccggtctc gacagcatcc cgggcggggg
      121 cggagagatc ctggtcgacg ccgtccgcgc gcgggtggcc aggaagaagg cccagaccga
      181 cgcgtggctg gaggtgcagg aagaagccca ccggcagggg atgaagacct cggtcacgat
      241 gatgtacggc ctgggggagt ccaacgccga ccgggtggag cacctcttcc gcgtccgtgc
      301 cgtccagcgg cggaccggcg ggttcacggc gttcatctgc tggccgctcc agcccgaggg
      361 caccccgatg ttcacgggcc aggccaagac cgacgctgtg acgtacctcc ggaccctcgc
      421 gatggcgcgg atcgtcctcg acaacgtgcc caacctgcag tcctcctggg tcacgatggg
      481 gcacaagatc ggccaggtgg cgctccggtt cggcgccaac gactacggct ccctgatgat
      541 ggaggagaac gtcgtctcgg ccgccggcac cacctaccgc accacgctta ccgagatcga
      601 gcggctcatc accgatgccg ggtacgcccc caggcgccgc cgccaggact actcgatcat
      661 cgaagccacg gccgcggcgt gagccgcacc gggatcgggt acgattccca tcgtttcgcg
      721 gccgggcggc cgctcatcct ggggggcatc accatcccct cggaccgcgg gctcaccggc
      781 cactcggacg ccgatgccat cgcccacgcc ctcaccgacg ccctcctcgg cgccgccgcc
      841 gcgggggata tcggccggct ctttcccgac acagacccgc agtggaaggg cgccgattcc
      901 atgacgctcc tggccgccgc ccacgccgcg gcgcaggcca aggggtggcg gttcgtccag
      961 ggtgacatca ccatcatctg cgagcgcccg aagctcgcgc cgcacctccc cgccatcgcc
     1021 gagcggctcg ccgccgtgct cggggtgaca cccgatgccg tcggggtcaa ggccaagacg
     1081 aacgagggga tgggctggat cggccgggcc gaagggatcg cggtgatggc cgtcgccacc
     1141 gtggagccgg tccgctgatc cacggcctcc tcgcctggct cacgggcctc ccggcgggcg
     1201 cggtctacct ggtcctcgcc ctcctcgccg cgctcgagaa catcttcccc ccggtccccg
     1261 ccgacaccgc cgtcgcgctc ggtgccttcc tgtcgcagca gggtgtcacg accccgtgga
     1321 cggtgttcac ggtcacgatc ctggccaaca ccgcctccgc tgctctggtg tacggtgtcg
     1381 cccgccggtt cgggccccgc tttgccgcga ccgggctcgg gcggagactc cttccggccg
     1441 atgcgctcgc cacgctcgag cggaagtacc tgcgctgggg cctgccggcc atcttcctct
     1501 cccggatgat ccccgggatc cgggcgatcg tcccgccgtt caccggcatc atcgggctct
     1561 ccccgcgccg ggcgctcctt ccgatcgccg ccgcgtcggc cgtctggtat ggattcgtga
     1621 cggtggtcgg cgtcgtcctc ggctctgaat gggatacgat ggtgcgcctc ctcggtcagg
     1681 tgaatcgggc cttggcggtg gtcggggccg tcgcggccct cgcgatcgcg accggccttc
     1741 ttgtgcggtc ccgtgcccgg cggcggcatc accagcagcc ggtcaccggc gagctggtcg
     1801 acgcggtgct ccagaaggcg ctgcacaagg gcccggccgc gtgacgcggg aggagctgga
     1861 gcgggcgttc ctcctcgagg gcttccgtga ccacctgtcg ctcgaggccg gcgcgtcgcc
     1921 caacacggtc gagagctacc tgcgggacct gaggcatctc gtcgaatttg cgggcgcggc
     1981 ggggttgacc gggccggacc ggctcactgc ccggacgctc cgtgacttcg tgttcgggct
     2041 gaaggacctg ggcctcgggc ctgccaccat ccggcgccat gtgtcggccg tccgcacgta
     2101 tttccgcttt gccgtgggtg aaggcgttgt cgccgccgat cccagcgatc gcctcgaggc
     2161 cccccgccgt gggcgcaagc tccccgacac cctctcggtc gccgacgtgt ccgcgatgct
     2221 cgaagcgccg ccgctcgacg acccgctggc gtggcgggac cgtgcgctcc tcgaactggc
     2281 ctacggcgcc gggctccggg tgtccgaact ctgcgggctc accccggcgg acctctggct
     2341 cgacgaccag gtggtgcggg tcttcgggaa ggggagcaag gaacggatcg tgccgctggg
     2401 acgaacggtg atcggcgcgg tggccgtgta cctgcgccag ctccgccccg agctcgacac
     2461 cggcgccagc gggaaccggc tcctgctcaa tttccggggc cggccgctgt cgcgcgtcgg
     2521 cgcgtggggg atcgtgaaga aggccgcgaa gcgcgccggc atccgcaagc gggtgacgcc
     2581 gcatacgctg cgccactcct tcgcgaccca cctgctcgag ggaggggccg acctccgggc
     2641 cgtccaggag atgctcgggc acgccgacct gtccaccacc cagatctaca cgcacgtcga
     2701 ccgggagtac ctgcggtcgg tgcatcgcca gttccacccg cgcggctagg cgcgcctcag
     2761 ctcgcgaaga tgtcccgtcg atagcggcgg cgctgcggcc cgagcacgaa cgacaccagg
     2821 agcccggcca ccagcgccga ggcgatcagc accatctgga acgccgtctc cactcccagg
     2881 atcgtctgcc ggtcgagcaa cgacgccaag ccccggaatc cgacgctccc cggcaccaag
     2941 agcaggacgc tcggcaccag cgtcaccgac gtgggtcggt cggtgagcca tgcgtagacg
     3001 ttgctcgcga gcccggccga cagggctccg acgaacgcac ccacctccgg tccgaggatg
     3061 ctggccccca ggatcccgcc atagtacgtg accaccccgg tgaggaggat ccagccggtg
     3121 tcccgcgggt tggcacggag gaggatcgtg aatgccagcg gcgcgagcag gagggcggcc
     3181 acgatcgtcc atcccgggag ggggacggcc gaagcgggca tcagcttgcc ggtgatgtac
     3241 gtggccgtct tggtgccgag cgccacgccg aaggcgatgc tgatgaagag catcatcgcg
     3301 ccggagaacc ttgccacgcc ggcgatgagg tgccgcgtcg cgagttcggt catcgcggtc
     3361 gtcagcgtga accccgggat caggaccagg agccccgaga gcgtcacggt atacacggtg
     3421 atgccgtgga cgaacttccc cgacacgacc gcgagcattg acgccacgaa cgcagccgcc
     3481 acggggaaca cgcgccccag tgccgggacc cggatggcca gggcgttcag gagcccgacc
     3541 acgagcccga tcaccgccga cacctcgacc tccaccgacc cgccacccag gaacctcgcg
     3601 ccgcatgccg agaggaggcc gaaggccacg acggtcacga ggtcgttgta ccggggcggg
     3661 gaagcgacga tctcatcgat ccgcgccgag cccgcgagcg ggggaatggc gccccgcagc
     3721 acctgacccg ccacttcgtc gaggtcggcc agcttgccga ggtcatcgtc cgccgcgccg
     3781 accctcatga ggaaggtgcg ttgctcgtcc tcggcgccga agccggcgaa gatgctcgtg
     3841 ggagtggcga agaactgtcc ggtgaggccc agccggtccg aggcccgttc catgagcgtc
     3901 tcgacccggt gggcggcata acccacgttg tggagcgcgc ggccgagccg gaggacgaat
     3961 ccgcgggccg ccttctcctc gtccgccgtc agggggcggg caccgccccg tccggggacc
     4021 tgatactctg aactgtgcgc cggggatgct gggtacatgg ggctaatcta ccgcgaccgg
     4081 ctggcgtatt ctcgtgcggg ctggggtgca tccggcgcgg caaaatggcc cggcagaggc
     4141 gccccgggtg ctgcgactgt caaataaatt gtcgacaccg actcgaaata atacaccgca
     4201 caactttcta agctgttgat tcctaatatc ttaacatctt ttgctgttgg tatcggatat
     4261 gcttaatgac ggggcgcagt ccaatcgagc cccggagcgt gtatgcgata tcttcaggtt
     4321 gcgcttgtca tcgtgatcgc cctgttggtc ggcccgcggg cggcgcgtgc ccagatgtcc
     4381 tgtgagggac cgaacggttg cagcctccag atgagggtca acgccatggt gctgtcgacc
     4441 cggatcccct ccgtggccag ggtggccgtc cagccagcgg gcccggccgg gcagacgcgg
     4501 gtggcgacgc gggccaatac cgggtggagc gtctccattt ccggcagtgc ggccaagggg
     4561 gactccgtgc aagttcgctc ccccgccagc ggcgggatgg tcaccccagt ggagacgcgc
     4621 cagggtggcc tcatgatcgt caccctcgcc gagaggtagc actgggtcct gaaaccttcc
     4681 ccgtgtagag ttcgtctcct attgcaacat ccggagaacc acgacgggga gggcgacacc
     4741 gtgcgcaact actggttcaa gatcgccggc ggcgcgctgg cgatcttcgc ggtcgggttg
     4801 gcgggcgtct acgccgtccg tggcatccac cgtcgggtgc acgatctcgc gaacggcacc
     4861 ggcgacatca cgatcccgct cctcgggatc gttccgctct cgatcggcaa tgtacggctc
     4921 ggcgcgctga acaagatcga gatccatcga gacgccccca agcacgtcga tggcttcacc
     4981 gtcctcgccc ggctcgacga ctcggtcgac gccgatcggt tcgccgactg cttcttcacg
     5041 ctgcaggatc ccacccggat caatgacaag accaccttca cctgcctgga ctccgtcccg
     5101 gcaggaatgc gccagttcgg cgatctgcgg ctgatcgaca gttcgggcga tgcggtgtcg
     5161 gaacgcccgc tgatcctcgc cgaaagcgac atcgccgacc tgcagaacgt gcacgggggc
     5221 gggaccccga acgccgacag catccgcgcg tcggtggaat cgatcggcgc tgcggcccgg
     5281 aagatgggcg atagcatccg gcgggagatg cgtgcccgct atggcaaggg atccaccggt
     5341 acggtgtacc agccgacccg tcccgatccg ccgatgcccc agaagcctcc ggctcagccc
     5401 gcaccaaacg ccaggccctg atcaccgccc ccgtcccggc accacggcac gggggcggat
     5461 cgttgtgggg tgggcattcc cattccgcgg ccgaccagcg agcgttcatc gtccgtgctc
     5521 cctccatcga cccgccgagc gccatgctcc ttgtcatcga caactacgac agcttcacct
     5581 ggaacctggt ccagtacatc ggcgagctgg gcgtcgagcc ggtggtgtat cggaacgatc
     5641 ggctgagcgt cgacgaggca ctcgcactgg ccccggaggc gatcctcgtc tctcccggac
     5701 cgtgcacgcc gcgcgaagct gggatcgccg tttcgctcat ccggcgggca gcgggacgga
     5761 tcccggtgct cggagtatgc ctgggtcatc aagccatcgg ggaagcgttc ggcgggcagg
     5821 tggtccgggc cgaccggttg atgcacggca agaccgcagc cgtccggcat gacggatcct
     5881 cgctcttcga tggggtaccc tccccggtca cagtgatgcg gtaccactcc ctcgtcgtgg
     5941 cgccagaccg gcttccaccg gaactcgagg tgacggcgtg gtcggccgat cgggggcggg
     6001 aggacgagat catggggctc cgccaccgcc agcatccgat ctggggcgtg cagttccacc
     6061 ccgagtcggt cggcacggag cacggccgac ggatcctggc caactttctt gccttggccg
     6121 ggatcccggc cggcagcgga cgctgagccc aaaccgattc aggccaaccg cttagctcag
     6181 cgtggccgac gatggaccac ccgcggcacg acttttgaca ggggaccgct gtcgtcgtat
     6241 aattggcgat g
//
LOCUS       DASUAP010000282         7116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_963024, whole genome shotgun sequence.
ACCESSION   DASUAP010000282 DASUAP010000000
VERSION     DASUAP010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963024"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3449
                     /locus_tag="VFU45_07555"
     CDS             <1..3449
                     /locus_tag="VFU45_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5358958.1"
                     /translation="RFLGAAPLAPLYLAVFLSAIRRGPAQGWVAFGLSAVVVLLRLTP
                     LVDLTRRPHPLEFAAVAAAVCVGIIVLAGRTHDAVERSRAREAELARLNRLYDALSQV
                     DQAIVWSGTRQELFDKVCQALVERGGFRMCWIGQADRESSRMSVLARHGDEHDYLGAV
                     EIYVDQRPEGRGPSGTAFHEGRPYVANDFRSDPATLPWRQAAARSGFRAVAAFPIRQQ
                     GMAWGVLAVYADVAGFFRDREVALLEEAATDLSYALDNFVRDAEHSAAEAAAMRERQL
                     TDSVVESTPGILYLYDEQGRFLRWNRNFLTVSGYTAEEMAGLRPQDFFRGDDVARVSA
                     RIAEVFARGESALEAGFVTKDGRVIPYYFTGRRAELDGRPCLVGMGIDISERVRAEDA
                     TRRSEERYHSTLDSIAEGAQILGPDWRYLYLNPAAERHNRRPNSELLGRTMPEAWPGI
                     EGTPVFALLRRCQEERVAAQGEIAFTFSDGEVRWFDVRAHPVPEGIFVLSVDVTAEHE
                     ARSALSRHAALQRIVGRVARIGGWQIELPGRTMSWSDEVCDMHEVPRGCRLSLEEGLA
                     MFAPESREPMAQALEACIAAGTPYDLELVKLTARGRRFWVRTIGEAVRDADGRIIRVQ
                     GAIQDINERKEAETRLAEQAALLDHARDAIIVRDLEHRVRYWNRSAERMYGWSAAEAA
                     GRPVTELFYRPPGTPEEFLRAMALLLEVGEWTGDLEQRTRDGNVVVVEGRWSLLRDPA
                     GTPTSVLAINTDVSHRKALERQLLRAQRLESIGTLAGGIAHDLNNVLTPIMMSIELLR
                     ADDTAEGRLETIDTIASSARKGAEMVRQVLSFARGVEGQRVPVDIPTLVKEVEKLVND
                     TFLKSIEVSARVAPDLPLVLGDPTQLHQVLLNLCVNARDAMPEGGTLLIVADRHQVDA
                     RYVAINPDARVGTYVTIEVSDSGEGMKPEVLDRIFDPFFTTKEQGKGTGLGLSTSLAI
                     VKGHGGFFRVRSTPGSGSQFMILLPAHSGEPVATAALVEAAPATGNGELVLVVDDEAA
                     IRDVTRRILEGNGYRVVLAGDGAAGLATYRRRRDEVAVVLTDMMMPLMDGDAVIAALR
                     KIDPRVPIIAVSGVVEGVAAGEVLAAAGRTRHLPKPYTTTELLRAVHGVLHPPDASTP
                     PA"
     gene            3538..4191
                     /gene="msrA"
                     /locus_tag="VFU45_07560"
     CDS             3538..4191
                     /gene="msrA"
                     /locus_tag="VFU45_07560"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5358959.1"
                     /translation="MTVRTARLAALGFLLVAAFPFSIGAAQPTPRPRAPRRATADTAV
                     FAGGCFWGVEGVFEHVKGVISATSGYAGGTTDAPSYEDVSSGTTGHAESVRVVFDPSR
                     ITYAQLLQVFFLVVHDPTELDRQGPDVGTQYRSLVLYRDPAQRQAAEAYVAELTRTRA
                     FDRPIVTRIQPLGGFYPAEAYHQHFMALHPDYPYIVYNDAPKLVRLRQRFPGWYRDS"
     gene            4476..5081
                     /locus_tag="VFU45_07565"
     CDS             4476..5081
                     /locus_tag="VFU45_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU5358960.1"
                     /translation="MANSVSHGFGFVAALMATPSLVRAALRRGDPTGVVAVSVFAATL
                     VFLYLASTLYHAVREGRAKRAFRVLDHGAAYLLIAGTYTPFTLVSLHGAWGWTLFAVV
                     WVLAILGIVLTAVGALRHPQASTALFLGMGWLIVVAIRPLWLHLPLDGFIWLVAGGMA
                     YTIGVGFLAMDRRIRYGHFVWHLLVLVGSACHYIAVLRYAA"
     gene            5159..5659
                     /locus_tag="VFU45_07570"
     CDS             5159..5659
                     /locus_tag="VFU45_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5358961.1"
                     /translation="MRRTTAAPTILLTLSLSSACAVAPKVDPIAEMHAIRAQDSRFSA
                     AAARLDVVGAAALYAPDATILPPGLPAVHGADAIRTMLAGLFTAPGLSITLRPQRIDV
                     AAAGDLATDQGEVEVGMNTAECPVIEVSKYLEVWRKVDGEWKLMYDTWNANAPAVPAP
                     AEAKKR"
     gene            6605..>7116
                     /locus_tag="VFU45_07575"
     CDS             6605..>7116
                     /locus_tag="VFU45_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEU5358962.1"
                     /translation="MHAIPQTMGQQFGRRSWAEPWRIKMVEPIRRISAEERARALEAA
                     SYNTFLLHADDVYIDLLTDSGTGAMSDRQWAGMMVGDEAYAGSRSFYRLEAAVQQHYG
                     YAHILPTHQGRGAEHLLSRAAITPGQFVPGNMYFTTTRLHQELAGGVFVDIIVDEAHD
                     TTSLHPFKGDV"
BASE COUNT         1064 a         2573 c         2422 g         1057 t
ORIGIN      
        1 gtcgcttcct cggcgcagct cccctcgccc ccctctacct cgccgtcttc ctctccgcca
       61 tccgcagggg accagcccag ggctgggtgg ccttcgggct cagcgccgtc gtggtgcttc
      121 tccgcctgac accgctggtc gacctgaccc gccggccgca tccactcgag ttcgcggcag
      181 tggcggccgc ggtatgcgtc ggcatcatcg tgctggccgg gcggacgcac gacgccgtcg
      241 agcggagccg ggcgcgtgag gccgagctgg cgcggctcaa ccgcctctac gatgcgctga
      301 gccaggtcga ccaggccatc gtctggagcg gcacgcgcca ggagctcttc gacaaggtgt
      361 gccaggcgct ggtggagcgg ggcgggttcc ggatgtgctg gatcggccag gccgaccgcg
      421 agagctcgcg gatgtcggtg ctggcccgcc acggcgacga gcacgactac ctcggtgccg
      481 tcgagatcta cgtggaccag cggccggagg gtcgggggcc ctccggcacc gcattccacg
      541 agggtcggcc ctacgtggcc aatgacttcc ggtccgatcc cgccaccctc ccctggcgtc
      601 aggcggcggc ccggagcggc tttcgtgccg tggccgcctt cccgatccgt cagcagggga
      661 tggcctgggg ggtgctcgcg gtctacgccg acgtggccgg gttcttccgc gaccgcgagg
      721 tggccctgct ggaagaggcg gccaccgacc tctcctatgc gctggacaac ttcgtgcggg
      781 acgcggagca ctccgctgcc gaggcggccg cgatgcggga gcgccagctc accgacagcg
      841 tggtcgagag cacgcccggg atcctgtacc tctatgacga gcagggccgc ttcctgcgct
      901 ggaaccggaa cttcctcacg gtgagcggct acacggcgga ggagatggcg gggctccgcc
      961 cccaggactt cttccgcggg gacgacgtgg cccgcgtgag cgcacggatc gcggaggtct
     1021 ttgcccgggg cgaatcggcg ctcgaggcgg ggttcgtcac gaaggacggg cgcgtcatcc
     1081 cgtactactt caccggccgc cgggcggaac tcgatggacg cccctgcctc gtcggcatgg
     1141 ggatcgacat cagcgagcgt gtgcgcgcgg aggacgcgac ccgccgcagc gaggagcgat
     1201 accactccac cctcgacagc atcgccgagg gggcacagat cctcggcccc gactggcggt
     1261 acctgtacct gaaccccgcg gccgagcggc acaatcggcg ccccaactcg gaactgctgg
     1321 ggcgcacgat gccggaagcg tggcccggga tcgagggaac gccggtcttc gcgctccttc
     1381 ggcggtgcca ggaggagcgc gtcgcggccc agggcgagat cgcgttcacc ttttcggacg
     1441 gggaggtccg ctggttcgac gtccgcgccc acccggtgcc ggagggcatc ttcgtgctct
     1501 ccgtggacgt caccgcggaa cacgaggccc gcagcgcgct ctcccggcac gcggcgctcc
     1561 aacggatcgt gggccgggtg gcgcgcatcg gaggatggca gatcgagctg cccggccgga
     1621 ccatgtcctg gtcggacgag gtgtgcgaca tgcacgaggt gccgcgcgga tgccggctct
     1681 cgctcgagga ggggcttgcg atgttcgcgc ccgagtcacg ggagccgatg gcgcaggcgc
     1741 tcgaggcgtg catcgctgcc gggaccccgt acgacctgga gctcgtcaag ctcaccgccc
     1801 gcgggcgccg cttctgggtc cgcaccatcg gcgaggcggt gcgggatgcc gacgggcgca
     1861 tcatccgggt gcagggggcc atccaggaca tcaacgagcg caaggaggcg gaaacgcgcc
     1921 tggcggagca ggccgccctc ctggaccacg cgcgggacgc gatcatcgtc cgggacctgg
     1981 agcaccgggt gcgctactgg aaccggagcg cggagcggat gtacggctgg tcggcggcgg
     2041 aggccgcggg ccggccggtc accgagctgt tctaccggcc cccggggacg ccggaggagt
     2101 tcctccgcgc catggccctc ctgctcgagg tcggcgagtg gaccggcgac ctggaacagc
     2161 gcacccggga cgggaacgtc gtcgtggtcg aggggcggtg gagcctcctc cgggacccgg
     2221 ccggcacgcc gacctccgtc ctggccatca acaccgacgt gtcgcaccgc aaggcgctcg
     2281 aacgccagct cctccgcgcg cagcgtctgg agagcatcgg gaccctggcc ggcggcatcg
     2341 cccacgacct caacaacgtc ctgacgccca tcatgatgtc catcgagctc ctgcgggcgg
     2401 acgacaccgc cgagggacgc ctggagacga tcgacaccat cgcctcgagt gcccggaagg
     2461 gcgcggagat ggtgcgccag gtgctctcct tcgcccgtgg ggtcgaaggc cagcgggtgc
     2521 ctgtcgacat cccgacgctg gtgaaagaag tggagaagct cgtcaacgac accttcctca
     2581 agtccatcga ggtgagcgcc cgcgtggcgc ccgacctccc gttggtgctc ggtgacccga
     2641 cccagctcca ccaggtcctt ctcaacctct gcgtcaacgc ccgggacgcg atgccggagg
     2701 gcggcacgct gctgatcgtg gccgaccgcc accaggtgga cgcgcgatac gtggccatca
     2761 accccgatgc gcgggtcggc acctacgtca ccatcgaggt gagcgacagc ggcgaaggga
     2821 tgaagccgga agtgctcgac cggatcttcg accccttctt caccaccaag gaacagggga
     2881 aaggcaccgg actcggcctg tccacctccc tggccatcgt gaagggccac ggcggcttct
     2941 tccgagtgcg cagcacgccc gggagcggca gccagttcat gatcctgctc ccggcgcact
     3001 ccggggagcc ggtcgcgacc gcagcgctgg tcgaagccgc ccccgcaacg ggcaacgggg
     3061 agctcgtcct ggtcgtggac gacgaggcag ccatccgcga cgtcacgcgg aggatcctcg
     3121 aagggaacgg ctaccgcgtc gtgctcgcgg gggacggcgc cgcagggctg gccacctacc
     3181 gccggcgccg ggacgaggtg gcggtcgtcc tgaccgacat gatgatgccg ctgatggacg
     3241 gggacgcggt catcgccgcg ctccgcaaga tcgacccccg ggtcccgatc atcgccgtct
     3301 ccggcgtggt cgaaggagtg gccgccgggg aggtgctcgc cgccgcgggc cgcacccggc
     3361 acctgcccaa gccctacacc accaccgaac tgctccgagc cgtccacggc gtcctccatc
     3421 cgcccgacgc cagtacgcca ccggcctgaa ggcccggcgg gctgccggcg cgcgggctcc
     3481 gacgaaaccg cccggcctcg gccggcgtca tctgggtgac ccgacccgga gcacaccgtg
     3541 accgtccgca ccgcccgcct cgccgcactc ggattcctcc ttgtcgcagc gttccccttc
     3601 tcgatcggcg ccgcgcagcc gaccccccgc ccacgggcgc ctcggcgcgc cacggccgac
     3661 accgccgtct tcgccggcgg ctgcttctgg ggcgtcgagg gggtcttcga gcacgtgaaa
     3721 ggggtcatct cggcgacctc cggctacgcc ggcgggacga ctgatgcccc gagctacgag
     3781 gacgtcagtt cggggaccac ggggcatgca gagtcggtcc gggtggtgtt cgatccctcc
     3841 cgcatcacct acgcgcagct cctccaggtc ttcttcctgg tagtgcacga cccgaccgaa
     3901 ctcgatcgcc agggacctga cgtcggcacc cagtaccgct cgctcgtgct gtaccgcgac
     3961 cccgcccagc gccaggcggc cgaggcgtac gtggccgaac tgacccgcac cagggcgttc
     4021 gaccggccca tcgtgacccg catccagccc ctcggcgggt tctatcccgc cgaagcgtat
     4081 caccagcact tcatggccct ccaccccgac tatccatata tcgtgtacaa tgacgcgccc
     4141 aagctggtcc gcctgcgcca gcgctttccc gggtggtacc gggactcctg acccggctgg
     4201 tggcggaacg atccgcggcg catattcgac gcacgtcgcc ggagcggccg cgggccaggg
     4261 tcgcgcgggc cgccggacgg cgggcgccct gggccccctg ccgcggagat gtcgaatgcc
     4321 caagtacgtc ttcgagcgca agatcccctg cgcgaggacg ggtccggccg accgcacggc
     4381 gcgggtcatc gcgatgatcg aaccggccac cgcggagtag tggcatcccc ccggggctcc
     4441 cggcgcacca agcgccgcag tcgctcggcg aagagatcgc caacagcgtg agccacggat
     4501 tcggcttcgt ggcggcgctc atggccacgc cgtcccttgt gcgggcggcg ctgcgccgcg
     4561 gcgacccgac cggcgtcgtg gcggtgagcg tctttgccgc cacgctcgtg ttcctctacc
     4621 tggcgtcgac gctgtatcac gcggtgcggg agggccgggc caagcgcgcc ttccgggtgc
     4681 tcgatcacgg cgccgcctac ctcctcatcg ccggcaccta cacgccgttc accctggtct
     4741 ccctgcacgg cgcgtgggga tggacgctct tcgccgtggt gtgggtgctc gccatcctcg
     4801 ggatcgtcct cacggccgtc ggtgccctgc gccatcccca ggcctccacg gcgctcttcc
     4861 tcggcatggg gtggctcatc gtcgtcgcga tccggccgct ctggctccac cttccgctcg
     4921 acggattcat ctggctcgtg gccgggggga tggcctacac catcggggtc gggttcctcg
     4981 cgatggaccg ccggatacgc tacggccact tcgtgtggca cctgctggtg ctcgtcggca
     5041 gcgcctgcca ctacatcgcg gtcctccgct acgccgcctg aacggccgcg cgcgggcacg
     5101 gcgcctacat tacctcccgc catcgcccgt cagccggcca cgccctgaag gagacctgat
     5161 gcgccgcacg accgccgccc cgacgatcct gctgaccctg tccctctcat ccgcctgcgc
     5221 cgtggcgccg aaggtggatc cgatcgccga gatgcacgcc atccgcgccc aggactcgcg
     5281 gttcagcgcc gcggcggcca ggctcgacgt cgtcggcgcc gcggccctct acgctcccga
     5341 cgcgaccatt ctgccgccgg ggctgccggc agtgcacggc gccgacgcca tccgcacgat
     5401 gctggcgggg ctcttcacgg cgccggggct gtcgatcacc ctccggccgc agcggatcga
     5461 cgtcgccgcc gcgggcgacc tggccaccga ccagggggag gtcgaggtgg ggatgaacac
     5521 cgcggagtgt ccggtcatcg aggtgtcgaa gtacctcgag gtgtggcgca aggtggatgg
     5581 ggagtggaag ctgatgtacg acacctggaa cgcgaacgcg ccggcggtac cggcgccggc
     5641 ggaggcgaag aagcggtgac ggcctgactc ggccggcctg acgtgcctgg atctgcggca
     5701 cgtgactggc cgaattccgt gcgcccacgg gaagatgtgt cgctcgccgg cattgaaccg
     5761 tcaccttggg cccgcaccac aaggaacgct gccgccgtta cgcctattga ctaccgggcc
     5821 gagaggaggg atctcatgat gcggagcccg tggtttcgga tcgtgtgtgg aatggcaggc
     5881 ctgaccgccg tgacggtggt gggatgggcg tgttcgtcaa cggggccgcg accgctcccc
     5941 accgtcctgg tgaccaacac gtcgtgcagc tatggccggt gcactaccct cgagattcga
     6001 gcgttcatct ggaaattcac catcccgcag gatccatcgg gcttcgaaaa gctcggcgag
     6061 gctccgcccg gacagacctg tctcaccttc cctcccaact ggacgctccg catcatcggc
     6121 cctgacacga caggtcacgt cgatacaacc ttcatgacgt gggcacccga cgatggaagc
     6181 gcaatctacc tcactgcgat tgactcagcc ctccgttact cagcctcgga cagcgctcag
     6241 agggacagtc tgagtcgggg gttgctgccc tactttgaca agtacaccgg caccactgtc
     6301 ggggagaccc cagacttctc tcctgggaca gcatcgggct gggccgtcag cttcccgagc
     6361 gcccctgtac gcgaagcgaa gctcacggtg gtaccagcat gcaatggctg atcgctgaag
     6421 cgcgcattcg gcctgaagca ccggggtgga actggtcagt cggctgacga tcgaggcgtg
     6481 aagcggccag cccaccacca ttatatgaac gccccttcaa ccggccttat ggctttcgtt
     6541 aatctcgccc ggctagcgta ggcggtccgt cctccgcccc cgctccttca aagccgagtg
     6601 cctgatgcac gcgatcccgc agacgatggg ccagcagttc ggccgccgca gctgggccga
     6661 accctggcgt atcaagatgg tcgagccgat ccgtcgcatc tccgccgagg agcgcgcccg
     6721 cgcgctcgag gcggcgagct acaacacctt cctcctccat gccgacgacg tctacatcga
     6781 cctgctgacc gacagcggca ccggcgcgat gagcgaccgg cagtgggccg ggatgatggt
     6841 gggcgacgag gcgtacgccg ggagccggag cttctaccgg ctcgaggcgg cggtgcagca
     6901 gcactatggc tacgcccaca tcctccccac ccaccagggg cgcggcgctg agcacctgct
     6961 gagccgggcc gcgatcacgc ccggccagtt cgtgcccggc aacatgtact tcaccaccac
     7021 ccgcctgcac caggagctgg cgggcggcgt cttcgtcgac atcatcgtcg acgaggcgca
     7081 cgacaccacg agcctccacc ccttcaaggg ggatgt
//
LOCUS       DASUAP010000283         4602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_240437, whole genome shotgun sequence.
ACCESSION   DASUAP010000283 DASUAP010000000
VERSION     DASUAP010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240437"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VFU45_07580"
     CDS             <1..1158
                     /locus_tag="VFU45_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358963.1"
                     /translation="MAQAIGIVTTAGPMPHDATVTEARLVQPAVSVGLGALSGHLQFR
                     GTLDLEGWTMPGGELTPGAYGEGYIDRRHPHTYAHELMLTAHDVLGRLGGPAHLSAAF
                     GKGFVAFGSDDPMSRPVALYPIDHHLAQILEREVAIAGLAAGPAALEVSWFNGDEPEY
                     PSEWPNGRRFGDSYALRGSVAPVRGVEAQVSYAEVKSPEHRDGAGPPQEKWSASVRVD
                     RTFGGRPAYALAEYARTSEASGAFLFHSLVLEGAVQAGPVHPYLRLERTERPEEARTG
                     DVFRTPRPPLDNSLLGITRWSVATAGASVTALTAGPRFSVRPFLEASVADVRAISGTF
                     DVRAWYGSNTILSLSLGVRLDWGMAGHRMGRYGTGIAAGGDGMEMPMMEGP"
     gene            1155..2594
                     /locus_tag="VFU45_07585"
     CDS             1155..2594
                     /locus_tag="VFU45_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358964.1"
                     /translation="MKGRILLALLAAVGACRDASAPSTATGSVPDGLIVVSGDRQGLD
                     SGATPSPLVVQLWNKGKPVAGAVVHWEVTRGGATISDSITVTDGSGTAAVTVTPGSAG
                     FGQGVEAGVAGVPPVDFSLTLRRPFQVLGGGNNVAERYTSDLWLAKGYGYTGTWGFRS
                     AAGNVIKVWQLGGGGAPALVGADTIAGVNTVSDLQVSPDSTLLVATGESGPGAGLYVY
                     GLTDPAHPALLAQWSIASATQGLHTGTLAEIGGQLYAFTARDPSAPSLLTFRVQPDSL
                     AKIVLVDSIAMPANYGIHDTFVRDGLLFVENWNSGMWIYDVGGGGLGGSPGHAVFIGS
                     VVTQGGEVHNAWWFHDPVTNEKRYVFIGQEGPDVIGVSSAGDIHVVDVSDLSAPVEVA
                     HFHLGTTEGPHNFWMDEQRQVLYAAYYNGGVVALDVSGTLSGDLGSREIAEIQPGGSG
                     DTYVWGVMLYGGSLYATDMVSGLWQLTVP"
     gene            2776..>4602
                     /locus_tag="VFU45_07590"
     CDS             2776..>4602
                     /locus_tag="VFU45_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5358965.1"
                     /translation="MRIISALLLSTLVAAPAAAQQTPTLDHFLLTTPDAAKAREYSTE
                     LSRVIHIAGSPRQVETADYVLRLMKSWGLDTSRVSYRVYLPWADSAVVELAGPTPTRL
                     ALAEPPVAGDTTSYLAQYPTVHGYTGSGDVTAPLIYVNYGLPVDYAQLDSQGVSVKGK
                     VVIARYGRSFRGIKAREAEKHGALAILMYSDPHDDGFVPGDVYPWGPMRGAAGVQRGT
                     VQNGDGDPSTPGWPSTPDAKRLDPSQMEIPHIPSVPIGYGNAQVLLQGLKGPGALRGW
                     QGGLPFHYHLGGTAAVKVRVAVWAETGARAWKTITNTFGTLKGTTWPDEMVITGGHRD
                     AWGPGTADNVAGVTSVMESARAWGEASRRGLRPRRTLVFATWDAEEWGDEGSTEWVEA
                     HEADLSAHAVGYFNQDAGGVGRSFGGSATASLQPLVREVTKLVPQPGDTVSIYAAWRR
                     RDNVADSLEPAMGDLGGGSDFAAFYNHLGIPSADWGFGGPSGNYHSQYDDEKWMETWG
                     DSGFLGHRAIAQVDALLLARMADSDVLPLDEANLASYLGSLVKGVEVRAARQGWRTDM
                     SALYRAIAAMHDAALGFANVRDAGMASSGPKGATAALAEANLL"
BASE COUNT          655 a         1584 c         1672 g          691 t
ORIGIN      
        1 atggcacagg ccatcgggat cgtcaccacg gcaggcccga tgccccacga cgccaccgtc
       61 accgaggcgc ggctggtcca gccggccgtg agcgtgggac tcggcgccct gtccggtcac
      121 ctccagttcc gcggtaccct cgacctcgag gggtggacga tgcccggcgg cgagctcacg
      181 ccgggggcct acggcgaggg gtacatcgac cggcgccacc cccacaccta cgcgcacgaa
      241 ctgatgctga cggcccacga cgtgctgggg cgcctgggcg gtccggccca cctctcggcg
      301 gcgttcggca aggggttcgt ggccttcggg agcgacgacc cgatgagtcg cccggtggcg
      361 ctctatccga tcgaccacca cctggcccag atcctggagc gggaggtcgc gatcgccggc
      421 ctcgcggcgg gtccggcggc gctcgaagtc tcgtggttca acggcgacga gcccgagtac
      481 ccgtcggaat ggcccaacgg ccggcgcttc ggcgattcct acgccctgcg cggctcggtg
      541 gcgccggtgc ggggcgtcga ggcgcaggta tcgtacgccg aagtgaagag ccccgaacac
      601 cgcgacggtg cggggccgcc gcaggaaaag tggagcgcgt cggtccgggt cgaccggacg
      661 ttcggcggcc gcccggcata cgccctggcg gagtatgcac ggaccagcga agcgagcggc
      721 gcgttcctgt tccacagcct ggtgctcgag ggtgccgtgc aggccggtcc ggtccatcct
      781 taccttcgcc tggagcgcac ggaacgacct gaggaagcgc ggaccgggga cgtgttccgg
      841 accccgcgcc cgccgctcga caattcgctc ctcggcatca cgcggtggtc ggtggccacc
      901 gccggcgcct cggtgaccgc gctcaccgcg ggcccccggt tctcggtgcg accattcctc
      961 gaggcgtccg tggccgacgt gcgtgcgatc agcggcacct tcgacgtccg ggcgtggtac
     1021 gggtcgaaca ccatcctgtc gctttccctc ggggtgcggc tcgactgggg gatggccggg
     1081 caccggatgg gccgctatgg gaccggcatc gctgcgggcg gcgacgggat ggagatgccg
     1141 atgatggagg gaccgtgaag ggtcgcatcc tgctcgcgct gctggccgcg gtcggtgcct
     1201 gccgggacgc gtcggcgccc agcaccgcga cgggctcggt tcccgacggc ctcatcgtcg
     1261 tctccggcga ccgccaggga ctcgattccg gtgccacgcc gtcgccgctg gtggtgcagc
     1321 tgtggaacaa ggggaagccg gtcgccgggg cggtcgtcca ctgggaggtg acgcggggcg
     1381 gagcgacgat ctccgacagc atcaccgtga ccgacggatc cgggaccgcc gcggtgaccg
     1441 tcacgcccgg ttccgcggga ttcgggcagg gcgttgaggc cggcgtcgcc ggggtgccgc
     1501 cggtggactt ttccctcacg ctccgccgcc cgttccaggt gctcggcggc ggcaacaacg
     1561 tcgcggagcg gtacacgtcc gacctgtggc tcgcgaaggg gtacggctac accgggacgt
     1621 ggggattccg ctccgccgcg gggaacgtca tcaaggtgtg gcagctcggc ggcggcggcg
     1681 ccccggcgct cgtgggggcg gataccatcg ccggcgtcaa cacggtgagc gaccttcagg
     1741 tgagccccga cagcacgctg ctcgtggcca ccggcgagtc cgggccgggt gccgggctct
     1801 acgtgtacgg cctgaccgac cccgcgcacc cggcactgct ggcgcagtgg tcgatcgcca
     1861 gcgcgaccca ggggctccac accgggacgc tcgccgagat cggcggccag ctctacgcct
     1921 tcaccgcacg cgatccgagc gcgccttcgc tcctcacctt ccgcgtccag cccgattcgc
     1981 tggcgaagat cgtgctcgtc gattcgatcg cgatgccggc caactacggc atccacgaca
     2041 ccttcgtgcg ggatggcctg ctgttcgtgg agaactggaa cagcgggatg tggatctacg
     2101 acgtcggcgg cggagggctg gggggctcgc cgggccatgc ggtgttcatc ggatcggtgg
     2161 tgacccaggg cggcgaggtc cacaatgcct ggtggttcca cgatccggtc acgaacgaga
     2221 agcggtacgt attcatcgga caggaggggc cggacgtcat cggggtctcc agcgccggcg
     2281 acatccacgt ggtggacgtc tcggacctct cggccccggt cgaggtggcg catttccatc
     2341 tcggcaccac cgaaggtccc cacaacttct ggatggacga gcagcgacag gtgctctacg
     2401 ccgcgtacta caacggcgga gtcgtggcgc tcgacgtctc gggcaccctg agcggcgacc
     2461 tggggtcccg cgagatcgcc gagatacagc ccggcggcag cggcgatacg tacgtctggg
     2521 gcgtgatgct ctacggcggt tcgctctacg ccacggacat ggtgagcggg ctctggcagc
     2581 tcacggtgcc gtgagggtcg gacgccatcc tgacacgcgg caaggtggaa cggtggcaag
     2641 atccgggtag cgttcctcgc gtgtcatcgc atatttggtc gttggtcgtt ggtcgttggt
     2701 cgttggtcgt tcgttgttcg tcgttggtca cgcaccacgc gccaccccca ccactccttc
     2761 ccgggatcac gatccgtgcg gatcatcagc gctcttctgc tcagcaccct cgtcgctgcg
     2821 ccggccgccg cgcaacagac gccgacgctc gatcacttcc ttctcacgac gcccgacgcg
     2881 gccaaggcgc gcgagtactc gaccgagctg agccgtgtca tccatatcgc cggctcgccg
     2941 cggcaggtgg agaccgccga ctacgtcctt cgcctgatga agtcgtgggg cctcgacacc
     3001 agccgcgtct cctaccgcgt ctacctgccg tgggccgatt ccgcggtggt ggagctggcc
     3061 ggcccgacgc cgacgcggct tgcgctggcg gagccgcccg tcgccggtga cacgaccagc
     3121 tacctggcgc agtaccccac ggtccacggg tacaccggct ccggtgacgt caccgcgccg
     3181 ctcatctacg tcaactacgg cctgccggtc gattacgccc agctcgactc ccagggcgtg
     3241 agcgtcaagg gaaaggtggt catcgcacgc tatggccgga gcttccgcgg catcaaggcc
     3301 cgcgaggccg agaagcacgg cgcgctcgcc atcctgatgt attccgatcc gcacgacgac
     3361 gggttcgtgc cgggcgacgt ctatccctgg ggcccgatgc gtggcgcggc gggggtccag
     3421 cggggcacgg tgcagaatgg cgacggcgat ccctcgaccc ccggctggcc gagcacgccg
     3481 gacgccaagc ggctcgaccc ctcgcagatg gagatcccgc acatcccgtc ggtgccgatc
     3541 ggctacggca atgcgcaggt cctgctgcag ggcctcaagg gaccgggggc gctccggggc
     3601 tggcagggcg ggctgccgtt ccattaccac ctcggcggga ccgccgccgt gaaggtccgc
     3661 gtcgccgtgt gggccgagac cggcgccagg gcgtggaaga ccatcaccaa caccttcggg
     3721 acgttgaagg ggaccacctg gcctgatgag atggtcatca ccggcggcca ccgcgatgcg
     3781 tggggccccg ggaccgctga caacgtggcc ggcgtgacga gcgtgatgga gtcggcgcgc
     3841 gcgtggggcg aggcgtccag gcgcgggctc cggccgcggc ggaccctggt gttcgccacc
     3901 tgggatgcgg aggaatgggg ggacgaaggc tcgaccgaat gggtcgaggc ccacgaggcc
     3961 gatctctcgg cccacgcggt cgggtacttc aaccaggatg cggggggcgt aggccggtcg
     4021 ttcggcgggt cggccaccgc gtcgctccag ccgctggtgc gcgaggtgac caagctcgtg
     4081 ccccagcccg gtgacaccgt ttcgatctac gcggcctggc gccggcggga caacgtggcc
     4141 gattccctcg agccggcgat gggcgacctg ggcggcggga gcgacttcgc cgcgttctac
     4201 aaccacctcg gcatcccgtc ggcggactgg ggattcggcg ggccgtcggg caactaccac
     4261 tcccagtacg acgacgagaa gtggatggag acgtggggcg acagcggctt cctgggtcac
     4321 cgcgccatcg cccaggtcga cgcgctcctg ctggcccgga tggcggacag cgacgtcctg
     4381 ccgctcgacg aggccaacct ggccagctat ctcggctcgc tggtgaaggg cgtggaggtc
     4441 cgcgcggccc ggcaggggtg gcggaccgac atgtcggcgc tctaccgggc catcgccgcg
     4501 atgcacgatg cggcgctcgg ctttgccaac gtgcgggatg ccgggatggc gtccagcggg
     4561 ccgaagggtg ccacggcagc gctcgcggag gccaacctgc tg
//
LOCUS       DASUAP010000284         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1316771, whole genome shotgun sequence.
ACCESSION   DASUAP010000284 DASUAP010000000
VERSION     DASUAP010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316771"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFU45_07595"
     CDS             complement(<1..225)
                     /locus_tag="VFU45_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU5358966.1"
                     /translation="MARIDVIMPQMGESIAEGTLSKWLKKVGDPVKRDEPIFEISTDK
                     VDAEIPAPSAGTLAEILVQEGQTVAIQTVVG"
     gene            complement(327..1304)
                     /locus_tag="VFU45_07600"
     CDS             complement(327..1304)
                     /locus_tag="VFU45_07600"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5358967.1"
                     /translation="MSEMTYLEAIRQGLFEEMERDPNVFCLGEDIGAYGGAFKVTEGL
                     LDRFGDRRVIDTPISEAAIIGAAAGAAHMGMRPVCEMQFVDFVSCGYDMLTNYVATAR
                     YRAFLPCPMVVRGPSGGYVRGGPFHSQNPEAAFLHTPGLKIVYPATASDAKGLIKSAI
                     RDDDCVLFFEHKYLYRRIKETLPEGDHLVPIGKARVAREGRDVSVITYAATVWKALEA
                     AEQLEKEDGLSVEVVDLRSLAPMDDDAIVATVKKTSRVLVLHEDTVTGGVAGEITARI
                     NELAFEWLDAPVRRICAHDVPLPYAPVLEDFVLPQTSDVVRGLRWLAAY"
     gene            complement(1297..>2083)
                     /locus_tag="VFU45_07605"
     CDS             complement(1297..>2083)
                     /locus_tag="VFU45_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5358968.1"
                     /translation="SPTRGRELNIHFGDTGRGFIGQISHLGDMVPVMAGVTLSFKLRG
                     EDRVGLVYVGDGATSTGAFHEGINLAAVQRCPLVVVVENNGYAYSTPTWKQTAAESFI
                     AKAPGYGIRGVREDGNDVLAVYEVTKDAVDRARAGEGVTLVELVTYRRKGHAEHDNQS
                     YVPPGERERWAAENDPLDRYQRVLTGAGFTAEDLAAVDARAAAEVDAATDEAEKSPPP
                     EARDALLGVLADPPSAPVLWFREDVRSAVERHERPSAWGTFDV"
BASE COUNT          310 a          773 c          664 g          336 t
ORIGIN      
        1 tcccaccacg gtctggatgg cgacggtctg cccttcctgc accaggattt cggccagcgt
       61 cccggcgctg ggcgccggaa tctcggcatc caccttgtcg gtcgagattt cgaagatggg
      121 ttcgtcccgc ttgaccggat cgcccacctt cttgagccac ttggagaggg tgccttcggc
      181 gatggactca cccatctgcg gcatgatcac gtcaatgcga gccatggtcc aggcctgtca
      241 catgctgggg ttcactgcgg gcggcgtccg acgcggccgc ggcccgggtt tccgtcaatc
      301 taaccggtca gggcgggtcg cgcggctcag tacgccgcga gccaccggag cccgcgcacc
      361 acgtccgatg tctggggcag cacgaaatcc tccagcaccg gcgcgtacgg cagcgggacg
      421 tcatgggcgc agatccgccg gaccggcgcg tccagccact cgaacgccag ctcgttgatc
      481 cgcgcggtga tctcccccgc cacgccgccg gtcaccgtgt cctcgtgcag caccagcacc
      541 cggctggtct tcttcacggt ggccacgatc gcgtcgtcgt ccatcggcgc cagcgaccgc
      601 aggtcaacca cttccaccga cagcccgtct tccttctcga gctgctcggc ggcttcgagc
      661 gccttccaca cggtcgcggc ataggtgatc accgagacat cccggccctc ccgtgccacc
      721 cgcgccttgc cgatcggcac caggtgatcg ccctcgggca gcgtctcctt gatgcgccgg
      781 tagagatact tgtgctcgaa gaagagcacg cagtcgtcgt cccggatggc ggacttgatg
      841 agccccttcg cgtccgacgc cgtggccgga tagacgatct tgaggcccgg cgtgtgcagg
      901 aatgccgcct cggggttctg cgaatggaac ggcccgccgc gcacgtagcc gccggacggc
      961 ccccgcacca ccatcgggca cggaaggaag gcgcgatatc gcgcggtcgc gacatagttg
     1021 gtgagcatgt cgtacccgca cgagacgaaa tcgacgaact gcatctcgca gaccggccgc
     1081 atgcccatgt gcgccgcccc cgccgccgcg ccgatgatcg cggcctcgct gatcggcgta
     1141 tcgatcaccc gccggtcgcc gaagcggtcc agcagcccct cggtgacctt gaacgctccg
     1201 ccgtaggcac cgatgtcctc gcccaggcag aacacgttcg ggtcccgttc catctcctcg
     1261 aacagtccct ggcggatcgc ctcgaggtag gtcatctcag acatcgaagg tcccccacgc
     1321 cgaggggcgt tcgtgccgct ccaccgccga ccgcacgtcc tcgcggaacc agagcaccgg
     1381 ggcggacggc ggatcggcaa gcacacccag cagcgcgtcg cgtgcctcgg gcggcgggga
     1441 cttctccgcc tcgtcggtcg cagcgtccac ctctgccgcc gcgcgcgcgt ccacggccgc
     1501 aagatcctcc gccgtgaagc cggcacccgt cagcacccgc tggtaccggt cgagcgggtc
     1561 attctccgcg gcccagcgtt cccgctcgcc gggagggacg taggactggt tgtcgtgctc
     1621 ggcatgcccc ttccgccggt aggtcaccag ctcgacgagc gtcacccctt cgccggcccg
     1681 cgcgcggtcc acggcgtcct tcgtcacctc gtagaccgcc agcacgtcgt tcccgtcctc
     1741 acgcaccccg cggatgccgt atcccggcgc cttcgcaatg aagctctcgg cggcggtctg
     1801 cttccaggtg ggcgtcgaat aggcgtagcc gttgttctcg accacgacca ccagcgggca
     1861 gcgctgcacc gccgcgaggt tgatcccctc gtggaaggcg ccagtggagg tggcgccgtc
     1921 accgacgtac acgaggccca cccggtcctc gccgcgaagc ttgaacgaca gcgtgacccc
     1981 ggccatgacc gggaccatgt cgcccaggtg ggagatctgc ccgatgaagc cgcgcccggt
     2041 gtcgccgaag tggatgttga gctcgcggcc gcgggtgggg ctg
//
LOCUS       DASUAP010000285        11005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_853682, whole genome shotgun sequence.
ACCESSION   DASUAP010000285 DASUAP010000000
VERSION     DASUAP010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11005
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853682"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VFU45_07610"
     CDS             <1..628
                     /locus_tag="VFU45_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5358969.1"
                     /translation="DPAVRVVVVRGAGKDFCAGADLEELLASAELDPAANEASARRLG
                     AVFERMRALPKPVVAAVHGRALAGGAGLATACDIVLAAESAEFGYPEIRRGFVPAMVM
                     AILRRLVGEKRAFELVASGRSVGAREALDLGLVSRVVADQKLDVALAEVADALAAQSA
                     TAFALTKRLFYEQDGRSFADGIALGARVNALSRTTPDFRAAIAAFLKR"
     gene            698..886
                     /locus_tag="VFU45_07615"
     CDS             698..886
                     /locus_tag="VFU45_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358970.1"
                     /translation="MPLLLRSRLYLAALGVVLGVVGIARDDRRMIWGAIGVLGAAVVL
                     RFVARALSARRDRASGGS"
     gene            complement(849..3752)
                     /locus_tag="VFU45_07620"
     CDS             complement(849..3752)
                     /locus_tag="VFU45_07620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5358971.1"
                     /translation="MTVLLHCPHCGGRMEPTDLRCRFCGSRRSVAVTAGAGLPSIVAD
                     ASDPILASELQETLAPGIVVLRELGRGGMGLVFLAREPALRRLVVIKVLAPELSQDAT
                     ARARFRREAEAAASVAHPNVVAVHQAGELPRSGTAYLVMQFIDGATLAEEFPSGVVAP
                     EAKARRVMGEVAGALAAAHARGLIHRDIKPANVMLERETGRAIVLDFGVSAARHSSQH
                     RAGPRITQEGVTLGTPIYMSPEQAAAEPVTDRSDVYSLGVVMFELVTGRAPFTERSPM
                     ALAAAHLHKAPPTVESKRPDLEPEFAGLIDRCLAKRPEERPDAAAIARALLPNAGTSV
                     EWPPPGLARLHGAGRRTLIRFRYATGAAFAYLVLVAWLGTSVAGPGWLLLTAGMVALA
                     FGLNFGAERANGRMLRWMSAGRQAGYPLRVLFDVALDRPADSGALMAGSGIFALTPAP
                     RRTVWIGRRRAAAVLLALGYVATGVTVVLWSMGIIAVGSGSAFRALTVGGALLLFAPS
                     IAAEAAAALLTWPEFRVRLTLGRRSESALTRWRTPVIRRDLAERWLAGTGAAALPPHS
                     RWRARTLRWLAGAWTLPAIALALVLVALTWANAFIRDPARAADFEPWDQWQQLAGPER
                     HVDPAWPAVVATVDTVLAGWGDAVPDTAAARSMLMDVFGPPKDARPEWANLRIRADST
                     AVAAAWAALPGRLPGAVAIRLANDTTGVAPGLAAFRRMARSPLPGPWYRDSRDPTPAT
                     ITARGAYRLVFDYALRNAAAAALLLDHGDRPHALERTREILGVARPMLQAPSAAFQND
                     GLRLTALAGRMLDVIGRRAGDPALAAHGDALVAASARALESGQQFPLRSASMAAADPS
                     PARWVAIAGDRQLSPATRWAAFQAVVDGSCWNEREVLSGPAAARAALLTAMRSGVADL
                     PDADPIYRTILVQLDWLRRPWTAVGGVDGGRIPGLFRPAIDLMIRPTLCRDVR"
     gene            3916..5745
                     /locus_tag="VFU45_07625"
     CDS             3916..5745
                     /locus_tag="VFU45_07625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEU5358972.1"
                     /translation="MAEARAVLQRHFGYPDLRPAQRRVVGSVLRGRDTLAILPTGGGK
                     SLCFQVPALCLPGFTIVVSPLIALMQDQVAALARRGISAAALNSTLGRDAQEAILDDV
                     ASGRMKLLYTSPERLERLTRELARRQLRPALLAVDEAHCITEWGHDFRPGFRRLARSR
                     WQLGAPPVVALTGSATPEVRADITSALKLGAWGRSRGVDVHLGSFDRPNLWFGALRVA
                     DRRERWNALLELLKRGDRMALVYAPTRNATEGLSRALVRAGLRAAPYHAGLTKAERAA
                     ALERFLGDEVEVVVATSAFGMGIDKPDVRLVVHWMMPPTLESYYQEAGRAGRDGAFAR
                     CILLVGDDDARIHRAQLDVTFPPTDLVERVWQEPGPHRGVAANTLASIERLRRELHPE
                     RGAVDWRPVRARRERTLARIAAMARYAVAGGCRRSELLRYFGERLHTCAGCDRCGAGA
                     APATLDDGTRQRLRALHRWQRGLGRGAVELVDAATLARLAGAPPTSASALADVPGVGP
                     VLAERYGAAILGALGVAVGVTADATAIDPVAAVLASWRAEVAAAAGIPPYAVITDRVL
                     ARIAELRPATRGELARVEGVGPRTLIKHGERIVDIVAAACRTA"
     gene            5836..7056
                     /locus_tag="VFU45_07630"
     CDS             5836..7056
                     /locus_tag="VFU45_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5358973.1"
                     /translation="MDDALYFSDQHREVREMVREFSRKEIAPVARELDRTAEFPWENI
                     RKMGELGLLGVPWSEELGGAGMDQLSYYITIHEMAKVDASHALTISAHTNLGTSPIVD
                     FGTDAQRRTYVPLLASGRVLGGFGLTEPGAGSDAGGTATTAVDKGDHYVLNGAKRFIT
                     HAGVGEVFVATARTSPGHGSRGITSFIVTKDTVDLARCQAVGVGHAPDLPKFKGVRAG
                     RKEDKMGWRASDTRELIFEDAIVPKENVLGNLGEGFVNFLKTLDGGRIGIAALSLGIA
                     EGAYEEALAYAGTRRQFGKFIGSFQGIHFQLADMALEIEAGKHLLYHAAWLKTHGRPF
                     KTEAAMAKLFCSELAMRVTTKAVQVFGGYGYTTDYPVERMMRDAKVCEIGEGTSEIQR
                     MVIARAILGEVING"
     gene            7053..7712
                     /locus_tag="VFU45_07635"
     CDS             7053..7712
                     /locus_tag="VFU45_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU5358974.1"
                     /translation="MSRPAGPHVTAIHAGPVREETFGGKTETTAICKVPIAGMARIGP
                     LGIEGDEQADARCHGGPSRALCCYVLEHQEAWAREWDRPVGPGSFGENLTVTGLTEAT
                     VHIGDRFRLGSALIEVASARGPCRTLAARLGVPDIVPRIRLNGWTGWYCRVLEPGEAT
                     AGAALVLEHAHPHAISVLDAYRIKLDKSGPRQPVERLLSVEALCPDWRKSLEARIGSG
                     D"
     gene            8186..10195
                     /locus_tag="VFU45_07640"
     CDS             8186..10195
                     /locus_tag="VFU45_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU5358975.1"
                     /translation="MKREILINGTQRETRVAILEDDRLVELLVDRPDHRRIVGDIYFG
                     RVEAVLPGIQAAFVDIGLEKSAFLHASDVLEPDDEHDPDEEPATSEYPVMVADAPARE
                     PEDAAPSSEEAEPAASEEPAADDAPGQPQGGGGQRRSRRRRRRRRGNGATGIEGEGEG
                     APEPAPTDGAAAEVVPGPGAVGESQDAQAAGPQGDPVAESSDAPARQQPRRDKEPRRQ
                     RGRGNGQQPQQPPQRERNGGPAPAPAPRQREVSRRGAIPNIADLVKKGDTFLVQVTKE
                     PISTKGCRVTKVISLPGRFLVYMPYASKVGVSRKIESREQRAKLRDMVSRLLPKDQGG
                     VIVRTVAEGTTEDHFKREIDSLLALWKKINKKMRFVRAPALIQRETSLTRGIIRDLFS
                     DKVDGLWCDSKELHHEIEQYLEQVDPELKQRAHLYTEAQPLFEKFDIEREIKDLFKPR
                     CELPNGGSIVVQPTEALVSIDVNTGRYTGKKDPEKTILRTNLEAAREIARQLRLRDVG
                     GIIVCDFIDMESRQNRERVLNELRAHLSRDRARTKAYAVSELGLIEMTRQRVRPSLWQ
                     SMTTECPTCSGTGRVFRPEVVARRLERSLKRAGYEHRDRQLAVRLHPEVALYLLEEEP
                     KLVQSLGRQIGIELEVRDDPMMRLDEFRLMSRPAGRDVTELYAVA"
     gene            10285..10599
                     /gene="rplU"
                     /locus_tag="VFU45_07645"
     CDS             10285..10599
                     /gene="rplU"
                     /locus_tag="VFU45_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU5358976.1"
                     /translation="MYAIFRALGKQFRAEKGKTLRLPLMDKQPGDKVVFDEVLLSSDG
                     ETIKAGTPLVKGAKVEAEVVGDVKGEKLYIFKFKRRKNYRRKTGHRQKFTDVKITGLK
                     LG"
     gene            10607..10870
                     /gene="rpmA"
                     /locus_tag="VFU45_07650"
     CDS             10607..10870
                     /gene="rpmA"
                     /locus_tag="VFU45_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU5358977.1"
                     /translation="MAHKKGVGSSRNGRDSNPQFLGVKRFGGEKVTSGMIIVRQRGTK
                     FHPGRNVRRASDDTLFSLIDGIVKFEHKDKARQKVSVYPVATN"
BASE COUNT         1634 a         3998 c         3883 g         1490 t
ORIGIN      
        1 cgatcccgcc gtccgggtcg tggtggtccg tggggcgggg aaggacttct gtgccggcgc
       61 cgacctcgag gagctccttg cctccgccga actcgacccc gccgccaacg aggcgtccgc
      121 ccgccggctg ggcgcggtct tcgagcgcat gcgggcgctg ccgaagccgg tggtggcggc
      181 ggtacacggc cgggcgctgg ccggcggcgc cggcctggcc acggcgtgcg acatcgtcct
      241 ggccgccgag agcgcggagt tcggatatcc ggagatccgc cgcgggttcg tgccggcgat
      301 ggtcatggcc atcctgcggc ggcttgtggg ggaaaaacgg gcgttcgagc tggtggcatc
      361 ggggcggtcg gtgggcgcgc gcgaagcgct cgacctcggg ctggtgagcc gtgtggtggc
      421 cgaccagaag ctcgacgtgg cgctcgccga ggtggcggac gccctcgcag cgcagagcgc
      481 taccgccttc gcgctgacca agcggctctt ctatgagcag gacggcaggt cgttcgccga
      541 cgggatcgcc ctcggcgccc gggtcaatgc cctctcccgt accacacccg atttccgcgc
      601 cgcgatcgcg gccttcctca agcggtgaac cgacgggggt cgcccggacc ctcgccgagc
      661 gcctgaccca ctgagcatcg ggatcatgga gcgcctcttg cccctcctcc tccgttctcg
      721 cctctacctt gccgccctcg gcgtcgtcct cggcgtcgtg gggatcgcgc gcgacgaccg
      781 ccggatgatc tggggcgcca tcggcgtcct cggagcggcg gtcgtgctgc gattcgtggc
      841 gcgcgccctc agcgcacgtc gcgacagagc gtcgggcgga tcataaggtc gatggccggg
      901 cgaaagaggc ccgggatgcg gcccccgtcc acccccccga ccgccgtcca cgggcgccgg
      961 agccagtcga gctgcaccag gatcgtccgg tagatcgggt cggcgtcggg caggtcggcg
     1021 acccccgacc gcatcgccgt caggagcgcc gcccgggcgg cggccggtcc gctcagcacc
     1081 tcccgctcat tccagcagct gccatccacc accgcctgga acgccgccca gcgggtggcg
     1141 ggagagagtt gccggtcgcc cgcgatcgcc acccaacgcg ccggagacgg gtcggcggcg
     1201 gccatcgacg ccgagcgcag cgggaactgc tgcccgctct cgagggcgcg ggccgacgcg
     1261 gccaccagcg cgtcgccatg ggcggcgaga gccgggtcgc cggcccgccg tccgatcacg
     1321 tcgagcatcc tgcccgccaa cgccgtgagc cggaggccgt cgttctggaa cgccgccgac
     1381 ggtgcctgca gcatcgggcg ggccacgccg aggatctcgc gggtccgctc cagtgcgtgc
     1441 ggccggtctc cgtggtccag gaggagggcg gcggcggccg cattccgaag ggcgtagtcg
     1501 aacaccaggc ggtaggcgcc gcgtgcggtg atggtcgccg gggtgggatc gcgggagtca
     1561 cgataccacg gaccggggag cggcgagcgg gccatccggc ggaacgcagc gagccccggg
     1621 gccacgcccg tggtgtcgtt cgccaggcgg atcgccaccg cgccggggag ccgccccggg
     1681 agcgcagccc acgccgccgc gaccgcggtg gagtcggccc ggatccgcag gttggcccac
     1741 tccgggcggg cgtccttcgg cggcccgaac acgtccatca acatcgagcg tgcggcggcg
     1801 gtgtcgggca ctgcatcgcc ccagccggcc aggacggtgt cgacggtcgc cacgacggcg
     1861 ggccacgccg ggtcgacgtg gcgctccggg cccgcgagct gctgccactg gtcccaaggc
     1921 tcgaagtccg cggcccgtgc cgggtcgcgg atgaacgcat tggcccaggt gagcgccacc
     1981 agcaccagcg cgagggcgat ggccggcagc gtccacgcgc ccgccagcca gcggagcgtc
     2041 cgcgcgcgcc agcgcgaatg cggcgggagc gccgccgcgc cggtgcccgc cagccagcgc
     2101 tcggccaggt cgcgtcggat cacgggggtc cgccagcggg tcagggccga ctcggagcgc
     2161 cgcccgagcg tgaggcgcac gcggaattcc ggccaggtca gcagcgccgc ggcggcttca
     2221 gccgcgatgc tcggtgcaaa gagcaggagc gcgcctccca ccgtcagcgc ccggaaggcg
     2281 gacccgcttc cgacggcaat gatgcccatg ctccagagca ccaccgtgac gccggtggcc
     2341 acgtatccga gcgcgaggag taccgcagcc gcccgccggc gcccgatcca cacggtccgg
     2401 cgcggcgccg gggtcagggc gaagatcccc gaaccggcca tcaaggcgcc cgaatcggcc
     2461 ggccggtcga gcgcgacgtc gaagagcacc cggagcggat accccgcctg gcgaccggcg
     2521 gacatccacc gaagcatccg tccgttggcc cgctcggcgc cgaagttgag cccgaacgcc
     2581 agcgccacca tgccggcggt caggagcagc caaccgggcc cggcgaccga cgtgccgagc
     2641 caggccacca gcaccaggta ggcgaatgcc gcgccggtgg cgtagcggaa ccggatgagc
     2701 gtccgacgtc cggcgccgtg cagccgggcg agccccggcg gcggccattc gacgctcgta
     2761 cccgcgttgg gcaggagcgc ccgggcgatg gccgcggcgt cggggcgttc ctccgggcgc
     2821 ttggccaggc aacggtcgat gagtcccgcg aactccggct ccaggtccgg gcgcttcgat
     2881 tccaccgtcg gcggcgcctt gtgcaggtgg gccgccgcca gcgccatcgg agatcgctcg
     2941 gtgaagggcg cccgcccggt caccagctcg aacatcacca cgcccaggct gtagacgtcg
     3001 ctccggtcgg tcaccggctc cgccgcggcc tgttccgggc tcatgtagat cggggtgccg
     3061 agcgtcaccc cttcctgggt gatgcggggg ccggcccggt gctgggagga atgccgcgcg
     3121 gcgctcaccc cgaagtcgag cacgatcgcc cgtcccgtct cccgctccag catgacgttg
     3181 gccggcttga tgtcgcggtg gatgagcccc cgggcgtgcg ccgccgccag tgcgcccgcc
     3241 acctcgccca tcacccgccg cgccttcgcc tccggcgcca ccacgccgct cgggaattcc
     3301 tcggcgagcg tcgccccgtc gatgaactgc atcacgagat aggcggtgcc tgagcgaggg
     3361 agttcccccg cctgatggac cgccaccacg ttcgggtggg ccaccgacgc ggccgcctcc
     3421 gcctcgcggc ggaagcgggc acgcgcggtg gcgtcctggg acagttccgg cgccagcacc
     3481 ttgatgacca ccaggcgccg gagcgccggt tcccgcgcca ggaacaccag ccccatcccg
     3541 ccgcggccga gttcgcgcag caccacgatc ccgggagcga gcgtctcctg cagttcgctc
     3601 gccagtatcg ggtcggacgc atcggcgacg atcgagggga ggccggcccc ggccgtcacg
     3661 gccacgctgc ggcggctgcc gcagaagcgg cagcggaggt cggtcggttc catccgacca
     3721 ccacagtgcg ggcagtgcag gaggacggtc atcaggggcg cgggttctcc ggaacctacg
     3781 gcgccccgcg tgccgggggc aaggtcgcgt tccccggcgc cgcgcgggat cgatttggtg
     3841 atgtacggct gcgtgggagc gttaccgtgt gccatgactc ttccggccgc cgtgatcccc
     3901 gccgccgttc ccgccatggc cgaggcccgt gcggtgctcc agcgccactt cgggtatccc
     3961 gacctccgtc ccgcccagcg gcgcgtggtc ggctcggtgc tccgcggccg ggacacgctc
     4021 gccatcctcc cgaccggcgg cgggaagtcg ctctgcttcc aggtcccggc gctctgtctc
     4081 cccggcttca ccatcgtggt ctccccgctc atcgcgctga tgcaggacca ggtcgcggcc
     4141 ctcgcccggc gcggcatctc cgcggccgcg ctcaacagca ccctcggtcg cgacgcgcag
     4201 gaggccatcc tcgacgacgt ggcgagcggc cggatgaagc tcctctacac ctcgcccgag
     4261 cgcctcgagc ggctcactcg ggagctcgcc cggcgccagc tccgcccggc cctcctcgcg
     4321 gtggacgagg cccactgcat caccgaatgg ggacacgact tccggcccgg gttccgccgc
     4381 ctggcccggt cgcgctggca gttgggtgcg cccccggtcg tcgcgctcac cggcagcgcc
     4441 acgccggagg ttcgggccga tatcacctcc gccctcaagc tcggcgcctg gggccggtcc
     4501 cgtggcgtcg acgtgcacct tggctccttc gaccggccga acctctggtt cggggccctc
     4561 cgcgtggccg accggaggga gcgctggaac gcgctcctcg agctgctcaa gcgcggtgac
     4621 cggatggcgc tggtctacgc cccgacgcgg aatgccaccg aggggctgag ccgcgcgctg
     4681 gtgcgggcgg ggctcagggc cgcgccgtac cacgccggcc tcaccaaggc tgagcgcgcc
     4741 gcggcgctcg agcgcttcct gggcgacgaa gtcgaggtgg tcgtggccac cagcgcattc
     4801 gggatgggga tcgacaagcc cgacgtccgg ctggtggtac actggatgat gccgcccacg
     4861 ctcgagagct actaccagga ggcggggcgc gccggacggg acggggcgtt cgcgcgctgc
     4921 atccttctcg tgggcgacga cgacgcccgg atccatcgcg cccagctgga cgtgaccttc
     4981 ccgccgaccg atctggtcga gcgggtctgg caggagccgg gaccgcaccg cggcgtggcg
     5041 gccaacacgc tggcatcgat cgaacggctc cgccgcgagc tccatccgga gcgcggcgcg
     5101 gtcgactggc ggccggtgcg cgcacggcgc gaacgcacgc tcgcccggat cgctgcgatg
     5161 gcgcggtacg ccgtcgccgg cggatgccgc cggtcggaac tcctgcggta cttcggcgag
     5221 cggctccaca cctgcgccgg atgcgaccgc tgtggtgcgg gcgccgcgcc ggccaccctc
     5281 gacgacggga cgcgccagcg gctccgcgcc ctccaccggt ggcaacgtgg actcgggcgc
     5341 ggcgcggtcg agctggtcga cgcagccacc ctcgcgaggc tggccggcgc gccaccgacg
     5401 tcagcgtcgg cgctggcgga cgttccaggt gtcgggccgg tcctcgccga gcgctacggt
     5461 gccgcgatcc tgggcgcgct tggcgttgcg gtcggcgtca ccgctgatgc cacagcgatc
     5521 gaccccgtgg ccgcagtgct tgcgagctgg cgggcggagg tcgcggccgc cgcggggatc
     5581 ccgccatatg ccgtcatcac cgaccgggtc ctggcgcgta tcgcggagct gcgaccggca
     5641 acccgcggcg agctcgcccg ggtcgagggc gtcgggcccc ggaccctcat caagcacggc
     5701 gagcgcatcg tggacatcgt ggccgccgcg tgcagaacgg cgtgactggg agcggtcggt
     5761 ccctcgctta tattccggcg atccctgcgg ttccttccgc gccggcgcgg cgcgccatac
     5821 ttccggtggc cattcatgga cgacgcactg tatttctccg accagcaccg cgaagtgcgc
     5881 gaaatggtcc gcgagttctc ccgcaaggag atcgccccgg tcgcccgcga attggaccgc
     5941 acggcggagt tcccgtggga gaatatcagg aagatgggcg aactcggcct cctcggtgtc
     6001 ccgtggagcg aggagctggg cggggcgggg atggaccagc tgagctacta catcaccatc
     6061 cacgagatgg ccaaggtcga cgccagccac gccctcacca tcagcgccca cacgaacctc
     6121 ggcacctctc ccatcgtcga cttcggcacc gacgcccagc gtcgcactta cgtgccgctc
     6181 ctggcctcgg gccgcgtgct cggtgggttc gggctcaccg agccgggcgc cggttccgat
     6241 gccggtggca ccgccaccac cgccgtcgac aagggcgatc actacgtcct gaacggcgcc
     6301 aagcggttca tcacccacgc cggcgtgggt gaggtgttcg tcgccaccgc ccgcacctcg
     6361 cccggtcacg gcagccgcgg catcaccagt ttcatcgtca ccaaggacac cgtcgacctg
     6421 gcccgttgcc aggccgtcgg cgtgggccac gccccggacc tccccaagtt caagggcgtc
     6481 cgcgccggcc ggaaagagga caagatgggc tggcgggcca gcgacacccg ggagctcatc
     6541 ttcgaggacg cgatcgtccc gaaggagaac gtcctgggca acctgggcga gggtttcgtc
     6601 aacttcctca agaccctgga cggggggcgg atcggcattg cggcgctctc gctcgggatc
     6661 gccgagggcg cgtacgagga ggccctggca tacgccggca cccgcaggca attcggcaag
     6721 ttcatcggaa gtttccaggg catccacttc cagctcgccg acatggccct cgagatcgag
     6781 gccggcaagc acctcctcta tcacgccgcc tggctcaaga cccacgggcg cccgttcaag
     6841 accgaggccg ccatggccaa gcttttctgc tccgagctcg ccatgcgcgt caccaccaag
     6901 gcggtgcagg tcttcggcgg gtatggctac accaccgact acccggtcga gcggatgatg
     6961 cgcgacgcca aggtctgcga gatcggcgag ggcaccagcg agatccagcg gatggtcatc
     7021 gcccgcgcca tcctcgggga agtgatcaac ggatgagccg gcccgccggc ccccacgtga
     7081 cggccatcca cgccggcccc gtccgcgagg agaccttcgg cggcaagacg gaaacgaccg
     7141 cgatctgcaa ggtgccgatc gccgggatgg cccgcatcgg cccgctcggg atcgagggcg
     7201 atgagcaggc cgacgcccgg tgccatggcg ggccctcccg ggcgctctgc tgctacgtcc
     7261 ttgaacacca ggaggcgtgg gcgcgggaat gggatcgccc ggtcggcccc ggatccttcg
     7321 gcgagaatct cacggtcacc gggctgaccg aggccaccgt ccacatcggc gaccggttcc
     7381 ggctcgggtc ggcgctcatc gaggtggcct cggcgcgggg cccgtgccgc accctggcgg
     7441 cgcggctcgg cgtccccgac atcgttcccc gcatccgcct caacgggtgg accggttggt
     7501 actgccgggt cctcgagccg ggggaggcca cggcgggcgc cgcgctcgtc ttggagcatg
     7561 cccaccccca cgccatctcg gtcctggacg cctaccggat caagctcgac aagtcaggtc
     7621 cccgccagcc cgtcgaacga ctcctctcgg tggaggcgct gtgtccggac tggcggaaga
     7681 gcctcgaggc ccgcatcgga tccggcgatt gatcggccag gccggtgtta tcttcggccg
     7741 acccacgacc cacgacccac gacccacgac ccacgaccca cgacccacga cccacgaccc
     7801 acgacccacg acccacgact attgccgtaa cccctcccat tgcatatatt tagctcgcct
     7861 atgaagtcag tcccccagcg ccagcggtcc cccttcatcc tgttgggcac acgcgccttg
     7921 ctgtgctcca tggacggagt gtccgcgttg tggccgggaa ttcggacagg gcaatcctca
     7981 gtcagggtcg gcgatcccaa ccggctgtac cggggtcgcg gaggtatggg tgtcgggcgg
     8041 ggcctggtcc ccgcgccggt gccggaaggt gcgggcccga tctcgggccg cgggtccaag
     8101 ggcggccgct tggcgtcata cgccgggtgg ctgcccgttc acttttctca ggccgtccag
     8161 tctggatcgg ccgagggtac agcgcgtgaa gcgcgagatt ctcatcaatg ggacgcagcg
     8221 ggagacgcgc gtcgccatcc tcgaggacga ccgccttgtt gaactcctgg tcgaccgtcc
     8281 cgaccaccgc cgcatcgtcg gcgacatcta cttcggccgg gtcgaggcgg tccttcccgg
     8341 gatccaggct gccttcgtag acatcgggct cgagaagagc gccttcctcc acgcgtcgga
     8401 tgtcctcgaa ccggatgacg agcacgatcc ggacgaggaa ccggccacct ccgaatatcc
     8461 ggtcatggtg gcggacgccc cggcccgcga gcccgaagac gccgcgccct cttccgaaga
     8521 ggccgaacct gcggcgtcgg aggagcccgc ggcggatgac gcgccgggcc agccgcaggg
     8581 cgggggcggg cagcgacgtt cgcgccgccg gcgccggcgt cggcgcggca acggcgccac
     8641 cgggatcgaa ggggagggcg agggcgctcc cgaaccggcc ccgaccgatg gggccgcggc
     8701 cgaggtcgtg ccgggtccgg gagcggtggg ggagtctcag gacgcgcagg cggccgggcc
     8761 gcagggcgac cctgtggccg agtcgagtga cgcgccggcc cggcagcagc cgcgccgcga
     8821 caaggagccg cgccgccagc gagggcgcgg gaacgggcag cagccgcaac agccgcccca
     8881 gcgcgagcgg aatggcggcc cggccccggc cccggctccg cggcagcggg aggtcagccg
     8941 ccggggtgcc atccccaaca tcgccgatct ggtcaagaag ggcgacacct tcctcgtcca
     9001 ggtcacgaag gaaccgatca gcaccaaggg ctgccgggtc accaaggtga tctcgctccc
     9061 ggggcgcttt ctcgtgtaca tgccgtacgc gtcgaaggtc ggggtcagcc gcaagatcga
     9121 gagccgggag cagcgcgcca agctccgcga catggtctcc cgcctgctcc cgaaggacca
     9181 ggggggcgtg atcgtccgga ccgtggccga gggcaccacc gaggaccact tcaagcgcga
     9241 gatcgactcg ctcctggccc tctggaagaa gatcaacaag aagatgcggt tcgtgcgcgc
     9301 gccggccctg atccagcgtg agacgagcct cacccgcggc atcatccgcg acctgttcag
     9361 cgacaaggtg gacgggctct ggtgcgattc gaaggagctc caccacgaga tcgagcagta
     9421 cctcgagcag gtggatcccg agctcaagca gcgcgcgcac ctctacaccg aggcccagcc
     9481 gctcttcgag aagttcgaca tcgaacgcga gatcaaggac ctcttcaagc cccgctgcga
     9541 gctccccaac ggcggctcga tcgtggtcca gcccaccgag gcgctggtgt cgatcgacgt
     9601 gaacacgggg cgctacaccg ggaagaagga tcccgagaag accatcctgc gcaccaacct
     9661 cgaggcggcg cgcgagatcg cccggcagct caggctccgg gacgtgggcg gcatcatcgt
     9721 ctgcgacttc atcgacatgg aatcgcgcca gaaccgggaa cgggtgctca acgagctccg
     9781 ggcgcacctg agccgcgacc gggcccgcac caaggcgtac gccgtctccg agctcggcct
     9841 gatcgagatg acccgccagc gggtccggcc gtcgctctgg cagtcgatga ccaccgaatg
     9901 cccgacctgc tcggggaccg gccgggtctt ccgcccggag gtggtggcgc ggcggctcga
     9961 gcgctcgctc aagcgggccg gctacgagca tcgcgaccgg cagttggcgg tccggctcca
    10021 cccggaggtg gcgctctacc tccttgagga ggagcccaag ctggtgcaga gcctggggcg
    10081 gcagatcggg atcgaactcg aggtccggga cgacccgatg atgcgcctgg atgagttccg
    10141 cttgatgagc cgcccggcag gccgggacgt gaccgaactc tacgccgtgg catgaccggt
    10201 cgggtgcagc acggcttgac ctaactgccg gctccgatta atcttccggg cttacaccgt
    10261 accccgtact cgggacagga tcacatgtac gcgatcttcc gcgccctggg caagcagttc
    10321 cgggccgaaa agggaaagac cctccgcctg ccgctgatgg acaagcagcc gggcgacaag
    10381 gtcgtcttcg acgaggtgct cctctcctcc gacggcgaga ccatcaaggc tggcaccccg
    10441 ctggtcaagg gcgccaaggt cgaggccgag gtcgtgggcg acgtgaaggg cgagaagctc
    10501 tacatcttca agttcaagcg ccggaagaac taccgccgga agaccggcca ccggcagaag
    10561 ttcactgacg tcaagatcac cggcctcaag ctgggctgag ggtaccatgg cacacaagaa
    10621 aggcgtcggc tccagccgca acggccgcga ttcgaacccg cagttcctcg gcgtcaagcg
    10681 cttcggcggc gagaaggtca cctcggggat gatcatcgtc cggcagcggg gcaccaagtt
    10741 ccaccccggg cggaatgtgc gccgcgccag cgacgacacc ctgttctcgc tgatcgacgg
    10801 catcgtcaag ttcgagcaca aggacaaggc ccggcagaag gtgtcggtct atccggtcgc
    10861 gacgaactga ccgcccgctc gagtccgaag ggcgccggct tcccggcgcc cttttctttt
    10921 gcccgtcacc tgtgcaatct gaaacatcga cgtcccgagt gcgtatgggg acgggactct
    10981 cagcgaggtg tccgatggcg acgtt
//
LOCUS       DASUAP010000286         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1330884, whole genome shotgun sequence.
ACCESSION   DASUAP010000286 DASUAP010000000
VERSION     DASUAP010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330884"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..1401
                     /locus_tag="VFU45_07655"
     CDS             364..1401
                     /locus_tag="VFU45_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU5358978.1"
                     /translation="MKFPSLDFGSFLPANEIAVDLGTANTLIYVKGEGIVLNEPSVVA
                     IEKATGKIKGIGLEAKRMLGRTPDGILAVRPLKDGVIADFDVTEKMLRYFLKTIIDKH
                     VFRVKPKVIVCVPSGITEVERRAVRDSAETAGAKQVLMVAEPMAAAIGVGLPVETPTG
                     NMVIDIGGGTTEIAVIALSGIVSDTSIRTGGDELDQAIVQFMRKNYNLLVGEPTAELI
                     KIKIGSAAPTGEEREMEVKGRDLVSGIPKTVRVHSSEIREAVQEPIQQIVDAVRRALE
                     ITPPELASDIVDRGIIMTGGGALIRGLDVLLQQETGLPIHVDEDPLTCVVRGTGRILD
                     DFEKYRNVLTN"
     gene            1423..2289
                     /gene="mreC"
                     /locus_tag="VFU45_07660"
     CDS             1423..2289
                     /gene="mreC"
                     /locus_tag="VFU45_07660"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5358979.1"
                     /translation="MSALARPSPFGPDRSTGRRDTVLALFSVAVSLTFLLGPASWGDT
                     AGSLIRASVLRPFLWLQGEAVAARTSRARFAALEAERDSVAFAAQQVPGLEAENDRLR
                     QLLGLAGRTTEPYVAAEVLRQPQLTDGRTLLVRAGADRGVRPFDPVVSPDGLVGVVQT
                     VEPTTSIVLTWAHPEFRVSTFVGDGAVAGIIASAELPLGSNPMLELRGIPYRDSVPLG
                     AAVLSSGLGGVYPKGIPVGRVAGVLREQPGWERIYLVRPATNLAAVSHVLILRGGADH
                     PVDAAFRPGTPP"
     gene            2286..2780
                     /gene="mreD"
                     /locus_tag="VFU45_07665"
     CDS             2286..2780
                     /gene="mreD"
                     /locus_tag="VFU45_07665"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU5358980.1"
                     /translation="MSGRESRVRLGLVFLLLLVLHFYVRPRFGDSRAAPDFLAMAVLL
                     FALRSAPGDAAVVGLCTGLLADALTPARFGAGALAHAVVGYLASWGRAVFFADNLVVT
                     AGFFAVGTWIRNLLVLLASGTSGADLLAQVAVWSTLQSLSTAACGTLLVVLLRGWLDI
                     RILE"
     gene            2777..>3014
                     /locus_tag="VFU45_07670"
     CDS             2777..>3014
                     /locus_tag="VFU45_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358981.1"
                     /translation="MKAFGSARLEARADAARWVLRGAFAILVAAFFGAQVVQHQRYTL
                     RAADNRLRAVPLTAPRGLILDRDGRVIAENVPGYA"
BASE COUNT          407 a         1097 c         1056 g          454 t
ORIGIN      
        1 tccgtgaggt cggggagctc ccgccgacgg ggcgggggac gggagggttc gggagtacgg
       61 ggaggtgatt cgtgagaggt gagacgtgag aggtgagagg gaagagggaa gaggggaagg
      121 ggtgagaccc gtgacccgtg acccgtgacc cgtgacccac gacccgtgac ccgtgacccg
      181 tgacccacga cccacgaccc gcgacacacg acccgtgacc cgcgacccgc gacacacgac
      241 ccgtgacccg cgaccctcga cctgcgaccc cccaccctct aaccccgcct cttgtcagaa
      301 gttacgccct gccgttacct taggctgtcc ccgcatcccc atcgctcccg acacggctgg
      361 tcgatgaagt ttccgtcgct cgatttcggt tccttcctgc ccgccaacga gatcgccgtc
      421 gatctcggca cggcgaacac gctcatctac gtgaagggcg aaggcatcgt gctcaacgag
      481 ccttcggtgg tcgcgatcga gaaggcgacc ggcaagatca aggggatcgg gctcgaggcc
      541 aagcggatgc tgggccgcac ccccgacggc atcctggccg tccggccgct caaggacggc
      601 gtcatcgccg acttcgacgt gaccgagaag atgctgcggt acttcctcaa gaccatcatc
      661 gacaagcacg tcttccgggt gaagccgaag gtcatcgtct gcgttccgtc ggggatcacc
      721 gaggtggagc ggcgcgcggt ccgcgactcc gccgagaccg ccggggccaa gcaggtcctg
      781 atggtggcgg agccgatggc ggccgcgatc ggcgtgggcc tcccggtcga gacgcccacc
      841 ggaaacatgg tcatcgacat cggcggcggc accaccgaga tcgcggtcat cgcgctgtcg
      901 gggatcgtct ccgacacctc gatccgcacc ggcggggacg agctcgacca ggcgatcgtc
      961 cagttcatgc gcaagaacta caatctcctc gtcggcgagc cgacggcgga actcatcaag
     1021 atcaagatcg ggtcggcggc ccccaccggc gaggagcggg agatggaggt caagggacgc
     1081 gacctcgtct ccggcatccc caagacggtg cgggtccact ccagcgagat ccgcgaggcg
     1141 gtgcaggagc cgatccagca gatcgtcgac gcggtccggc gggcgctcga gatcacgccg
     1201 cccgagctcg ccagcgacat cgtggaccgc ggcatcatca tgaccggcgg cggcgccctc
     1261 atccgggggc tcgacgtcct gctccagcag gagacgggtc tcccgatcca cgtcgacgag
     1321 gacccgctca cgtgcgtggt ccgcggcacc gggcgcatcc tcgacgactt cgagaagtac
     1381 cgcaacgtcc tcaccaactg acgcccttcc gagcgcccgc gcatgagcgc cctcgcccgt
     1441 ccgtcgccgt tcgggcccga ccgctccacc ggccggcgcg acacggtgct ggcgctcttc
     1501 tccgtggccg tctcgctcac gttcctgctg ggccccgcca gctggggtga caccgccggg
     1561 tctctgatcc gggcgtcggt gctgcgcccc ttcctctggc tccagggcga ggcggtggcg
     1621 gcccggacga gccgcgcccg cttcgcggcg ctcgaagccg agcgggattc cgtggcgttc
     1681 gcggcgcagc aggtgccggg gctcgaggcc gagaacgacc ggttgcgtca gctcctcggc
     1741 ctggccgggc ggaccaccga gccgtacgtc gccgccgagg tgctgcgcca gccccagctg
     1801 accgatggcc ggacgctcct ggtgcgggcc ggcgccgacc gcggagtgcg ccctttcgac
     1861 ccggtggtga gcccggacgg cctggtgggc gtggtccaga ccgtcgagcc gacgaccagc
     1921 atcgtgctca cctgggccca ccccgaattc cgggtcagca ccttcgtggg cgatggcgcc
     1981 gtggcgggca tcatcgcctc ggcggagttg ccgctgggca gcaatccgat gctcgagctc
     2041 cgcggcatcc cctaccgcga cagcgtcccg ctcggcgccg cggtgctctc ctcggggctg
     2101 ggcggggtgt atcccaaggg gatcccggtg gggcgcgtcg ccggcgtgct gcgggagcag
     2161 ccggggtggg agcggatcta cctggtccgg ccggcgacca acctggccgc ggtgtcgcac
     2221 gtgctgatcc tgcgcggcgg cgccgaccac ccggtggacg cggccttccg cccgggcacc
     2281 cctccgtgag cggtcgcgag tcgcgcgtcc ggctggggct ggtgttcctg ctcctcctgg
     2341 tgctgcattt ctacgtgcgg ccccgcttcg gcgactcgcg tgcggcgccg gacttcctcg
     2401 ccatggcggt gctgctcttc gcactccgga gcgcgccggg cgatgccgcc gtggtggggc
     2461 tctgcaccgg gctcctggcc gacgcgctca ccccggcccg gttcggcgcc ggcgcgctgg
     2521 cccacgcggt ggtgggctat ctcgcctcgt ggggccgcgc ggtgttcttt gccgacaacc
     2581 tcgtggtgac ggccgggttc ttcgcggtgg ggacctggat ccggaacctc ctcgtgctcc
     2641 tggccagcgg caccagtggg gcggacctcc tggcccaggt cgcggtctgg tccaccctcc
     2701 aatcgctctc cacggcggcc tgcggcaccc tgctggtggt gctgctgcgc ggctggctcg
     2761 acatccgcat cctcgaatga aggcgttcgg cagcgcgcgg ctcgaggcac gggcggacgc
     2821 ggcccgctgg gtcctgcgcg gtgcgttcgc gatcctcgtc gccgcgttct tcggcgcgca
     2881 ggtggtccag caccagcgct acaccctccg cgccgccgac aaccggctcc gcgcggtgcc
     2941 gctcaccgcg ccgcgcggcc tcatcctcga ccgcgatggc cgggtcatcg ccgagaacgt
     3001 cccgggctac gccg
//
LOCUS       DASUAP010000287         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_676763, whole genome shotgun sequence.
ACCESSION   DASUAP010000287 DASUAP010000000
VERSION     DASUAP010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676763"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..471
                     /locus_tag="VFU45_07675"
     CDS             25..471
                     /locus_tag="VFU45_07675"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEU5358982.1"
                     /translation="MAPPLSIALVGTWELRSREDRTHTGERRVDPALGADPVALLVYD
                     AHGRFTAQFMKRDRNEPAPEAAAAAGPNNSRAQGGYDAYFGTYTVDDAAGTVTQRLTG
                     ALSPENVGQVLTRAMTVAGNELTIELETTALDGVPIIRTLRWDRVT"
     gene            596..922
                     /locus_tag="VFU45_07680"
     CDS             596..922
                     /locus_tag="VFU45_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358983.1"
                     /translation="MSNVHVVRVPPTLARGRLCRLVEVSDGFLLVQVWSGGHWRAEPT
                     ASISPTDVRNGIDPTPRILRALGVPENDWSLVMPARRGVSALVALWSILELAGALTPV
                     GLTMAA"
     gene            1029..2219
                     /locus_tag="VFU45_07685"
     CDS             1029..2219
                     /locus_tag="VFU45_07685"
                     /inference="COORDINATES: protein motif:HMM:NF005761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain"
                     /protein_id="HEU5358984.1"
                     /translation="MPGPASDILISGLGIAGPTLAYWLRKRGFRPVLVERAPAPRRGG
                     YIIDFWGVGYDVADRMGLLPALHREACHLDEVRLVDTDGRRVARLRAAVFGQATGGRY
                     LSILRSGLARQVWDLVDGSVETIFGDSVTRIEQDADSALVHFEHAPPRRFGLVIGADG
                     LHSNIRSLAFDPSAPVQYHLGCCTAALTARAYPRRDTGVYVSYAAPGIQAARYALADG
                     ASTFFFIFVHKDPDDIPHHDAEAQKALVRRAFAGRGWECDQMLAEIDRTDDWYFDAVA
                     QVRIPQWHRGRVALVGDAAYCPSLLAGQGSALAMAGAYVLAYELERAAGDHATAFARY
                     QARFKPFIDSKQRAAEHFTWWMAPQTRLGVHLRNFSTRLMDLPIVAAATVKRSFGDPV
                     TLPD"
BASE COUNT          317 a          889 c          810 g          376 t
ORIGIN      
        1 gcggccgccc gcggggcctg agcggtggcg ccaccgcttt ccattgccct cgtcggcacc
       61 tgggagctcc gctcccggga ggaccgcacc cacaccggcg agcggcgcgt cgatcccgcc
      121 ctcggcgccg acccggtggc gctgctggtg tacgacgcgc acggccgctt caccgcccag
      181 ttcatgaagc gcgaccggaa cgagccggcg ccggaggccg ccgccgcggc cggacccaac
      241 aacagccggg cccagggcgg ctacgacgcc tatttcggca cgtacaccgt ggacgatgcg
      301 gcggggaccg tgacccagcg cctgaccggc gcgctctcgc cggagaacgt gggacaggta
      361 ctcacccgcg cgatgacggt ggccggcaac gagctcacca tcgagctcga gaccacggcg
      421 ctggacggcg tgcccattat ccgcaccctc cgctgggacc gggtcacctg aggagcgggg
      481 ctgccgtccg atcgttcagt tccggttgaa cgtgactcgc cgcgggccgg cgctgtcgct
      541 tgcgttccgg gggcgcggtc gccatcgtcc cagcccccct tcaggtgagc catacatgtc
      601 gaacgttcac gtggtccgcg tccccccgac attggcccgc ggccgcctgt gccggctggt
      661 cgaggtgtcg gacggattcc tgctcgtcca ggtctggtcg ggtggccatt ggcgtgccga
      721 gccgacggcg agcatttcgc ccacggacgt gcgcaacggg atcgacccga cgccccggat
      781 cctccgcgcg ctcggtgtgc cggagaatga ctggtcgctc gtcatgcccg cgcgccgtgg
      841 cgtcagtgcc ctcgtggccc tctggtccat ccttgagttg gccggtgcgc tcacgcccgt
      901 aggcctcacg atggccgcgt gaggccggcg ccggcccgta cctccatcgg agtcgcgagc
      961 ctggcaccgg ggaccaatgt cacgctgacc ggcatcgacg cgggtgcgat gcccgaacag
     1021 accaactgat gccgggcccg gcctccgaca tcctcatttc cggcctcggc atcgccggac
     1081 ccaccctcgc ctactggctc cgcaagcgcg gcttccgccc cgtcctggtc gaacgcgccc
     1141 ccgccccccg gcggggcggg tacatcatcg atttctgggg cgtgggttac gatgtggccg
     1201 accggatggg gctcctgccc gcgttgcacc gggaggcgtg ccacctcgac gaggtccgac
     1261 tggtcgacac cgacggccgc cgcgtggcgc gcctccgtgc ggcggtgttc gggcaggcga
     1321 ccggcggacg ctatctcagc atcctccgaa gcggcctggc acggcaggtg tgggacctgg
     1381 tggacgggtc ggtcgagacg atcttcggtg attcggtgac gcggatcgag caggacgccg
     1441 acagcgccct cgtgcacttc gagcacgccc caccccggag gttcggcctc gtgatcggag
     1501 ccgacgggct ccattcgaac atccgctcgc tcgcgttcga tccctcggcg cctgtccagt
     1561 atcacctggg atgttgcacc gcggccttga ccgcgcgtgc ctacccccgg cgtgacacgg
     1621 gcgtgtacgt gagttatgcg gcgcccggca tccaggccgc gcgatatgcg ctggcggacg
     1681 gggcatccac cttcttcttc atctttgtcc acaaggaccc ggacgacatc ccccaccacg
     1741 acgccgaggc gcagaaggcg ctcgtgcgcc gggcctttgc ggggcgaggg tgggagtgcg
     1801 accagatgct ggcggagatc gaccggaccg acgactggta cttcgacgcg gtggcgcagg
     1861 tccggatacc tcaatggcac cgcgggcggg tggccctcgt gggcgacgcg gcctactgcc
     1921 cgtcgctcct cgccggccag ggatcggcgc tcgcgatggc gggcgcatac gtgctggcgt
     1981 acgagctcga acgtgccgcc ggtgaccacg ccaccgcctt tgcacgatac caggcccgct
     2041 tcaagccctt catcgattcc aagcagcgtg cggccgagca cttcacctgg tggatggccc
     2101 cccagacgcg gcttggcgtg cacctgcgca acttctcgac ccgcctgatg gaccttccga
     2161 tcgtcgccgc cgccaccgtg aagcgttcct tcggcgatcc cgtcacgctg cccgactgaa
     2221 tcggtgagcc acctgccgtc ccggggccgg accgcggcgc tcaccgcgac cgcgctcctc
     2281 gccttcgcgg ccaactcgct catctgccgg gtggcgctcc gcagcggctc gatcgacgcc
     2341 gccagcttct cgacggtgcg catcgccacg ggtgcggcga tgctgctgct cc
//
LOCUS       DASUAP010000288         7491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_812946, whole genome shotgun sequence.
ACCESSION   DASUAP010000288 DASUAP010000000
VERSION     DASUAP010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812946"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFU45_07690"
     CDS             complement(<1..373)
                     /locus_tag="VFU45_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEU5358985.1"
                     /translation="MADRKREDALRYHEEGRPGKIEVVPTKRFQNQRDLSLAYTPGVA
                     EPCLAIKDRPEDAYRYTARGNLVAVVTNGTAVLWLGNIGPLAGKPVMEGKGVLFKAFA
                     DLDVFDLEIDALKPDDVIRFCQ"
     gene            complement(376..1089)
                     /locus_tag="VFU45_07695"
     CDS             complement(376..1089)
                     /locus_tag="VFU45_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358986.1"
                     /translation="MRRRGLLALAVAAAGVAVACAELSSPLRPAGYGFTLVARDSMTA
                     DDTVNGVVYHQDSVYTDTVWFHWPTSRLPVRIWADTSGGLGLPGHVRDGIALWKQVLQ
                     YGEYDATLVSDSTNADVLVYAGVPPVLPAAVRMRSMAPQCSGATDVVVSRPDHRKLLL
                     PIRIYVENKYPLGQDSTTWCLGLTVAHELGHSMGLFQHSDSLDDLMSSNPLVPVPTAR
                     DVSTVLFLYHFRPDLVPAR"
     gene            complement(1086..1703)
                     /locus_tag="VFU45_07700"
     CDS             complement(1086..1703)
                     /locus_tag="VFU45_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358987.1"
                     /translation="MLNILHPALRRLAAGPAFAIALAPGVLAAQVQWTGRIGVVASTK
                     IVSDAIVSPLTITPAVAPVLLLGGSIPLDAKGRRFDIELGLASGSYASKENGVTTDLG
                     TLRTATLTFGAEGQVAPRLRWRAALGFITYLPAQKTGIFQDGAPTRAAGGLGLEYRRA
                     WRPGWDLSAALRWDYHRFTTTHLQSQGYTGSEDVHRITLAVGVAH"
     gene            complement(2025..2903)
                     /locus_tag="VFU45_07705"
     CDS             complement(2025..2903)
                     /locus_tag="VFU45_07705"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEU5358988.1"
                     /translation="MAATKKVTAAELLGAHVSTAGGPQTAPPRGVEIGATAIQLFTKT
                     PNQWREPVLSEATVAAYRTAWAESGLGVAVSHDSYLINLASPDAALRKRSIASFTAEL
                     TRCTQLGVPYVVSHPGNFMDDRDAGLTRNAQAYAECLEAVPGPVMVLMETTAGTGTAL
                     GSTFEELAELRARMPKAVQARVGYCADTCHLYAAGYDLVNDYDGVWERFDRVCGLERL
                     RCLHLNDSKTPFASHRDRHELIAEGSLGAGPFKRIMNDPRMAGVAKILETPKSEDMVT
                     NDRKMLARLRGYVGKR"
     gene            complement(2882..4135)
                     /locus_tag="VFU45_07710"
     CDS             complement(2882..4135)
                     /locus_tag="VFU45_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5358989.1"
                     /translation="MSRRRGAGTEGRGAWRRAAFLTLLALCALAPRHLSAQGTIQQRM
                     QDSRKRLEQIRAERERLQQQRSDLQGQAHDAESELVNITRQRDATNRIVNELDRQIGG
                     LNQELDQVSASLALAQDNLADKRAVLYRRLVEIYKRGPLYDFQALLAAESFGDLVSRF
                     KYLYLGSRQDRQLVSDVERLRDRVNRQRDDLLNYRASLTTSREERDQELQHYGDLADE
                     QRSRLRDVRRDTRSTESRLSALQRDEEAMNALIATLERARREAAARAAAARASGRRVA
                     PPRSSITTASIGKLGWPMDGTVLYGFGTDELNSGAVIKHNGIAIAAPPGTPVKAVEAG
                     TVEMVQPLGTYGLSVVLSHGGGYYSIYSQLSTSSVTKGQAVVRGQMIGTSGGANTDEG
                     PHLYFEIRGENGVALDPTEWLQRRK"
     gene            complement(4128..4994)
                     /locus_tag="VFU45_07715"
     CDS             complement(4128..4994)
                     /locus_tag="VFU45_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEU5358990.1"
                     /translation="MRLLLREALLSFRRTPLLSMLSVTTIGFSLFVVGLFGLVAVNLR
                     QALKGVEERVEIVAYVLRGTPAEVIAQASTDIAAYPEVQQVTYVSEDVALARARRELT
                     EFRDAYRDLQANPLPASLEVTLKDGFRDEAAVTRVAERIKGYQFVDDVRFGRDWIAKL
                     DRIRDIAGLVGFVIGLAFAIVAVVIIGTTIRMAVLQRGREIGIMRLVGASNWFVRGPF
                     LLEGAVKGLLGGLLAIGLCWLAFSAVQGNAGLFSGLVFFEPDYLGAIVLFGTAIGFGG
                     SLFSVGRHLKDV"
     gene            complement(4991..5695)
                     /gene="ftsE"
                     /locus_tag="VFU45_07720"
     CDS             complement(4991..5695)
                     /gene="ftsE"
                     /locus_tag="VFU45_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU5358991.1"
                     /translation="MIQFTSVFKAYPKGHFGLRDVSLRVHKGEFVFLTGHSGAGKSTI
                     LKLMFAEQRPTEGEVRVSGFNIGTLSRGDVPKLRRRLGVVFQDFRLLEDRTAHENVAF
                     ALEVTGAKRDTIPGRVMRVLTQVGLAAKGQAYPRELSGGEQQRVAIARALVNDPAILL
                     ADEPTGNLDERATRGVFQLLRDINAGGTCVVMATHDLDLVKSAPYRTIEMREGMVVYD
                     SGEERIPAAEDLEGGR"
     gene            complement(5760..6305)
                     /locus_tag="VFU45_07725"
     CDS             complement(5760..6305)
                     /locus_tag="VFU45_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358992.1"
                     /translation="MVTTIRLETLLQEAHPARAPKNLVTRPTGAAVRNSIEREMTLRG
                     ARVALLDFSELDLVDLSCADEVVAKLLQRMDVTDERYVVLTGLTEDQAEAIDHVLSHH
                     QLGVQAITRDSTRPRLLGHFGNDSRAAFDLLIDRGPLDGGALARELGWRTERVADALQ
                     VLCLLGVVMANGGAYGAVMVA"
     gene            complement(6305..7204)
                     /locus_tag="VFU45_07730"
     CDS             complement(6305..7204)
                     /locus_tag="VFU45_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5358993.1"
                     /translation="MTRIVEIPSHLDDRTFEQFAASFAAGPATDKMLFDARGSKWSSP
                     YGFTGMLIAGQAVAQAGGPRPLFTVPEDEQARSYWTRIGFFREAAELFELHGKVPRAR
                     PQEESDVLLDVTPVRASEDVHAVVERVQDRSARIVTGELGLEAKAVMGFAMALSEACQ
                     NIVEHAGTTGWVAIQAYNWRKRLGRRVVVIAVADGGVGFRKSLEATQAKRIGERWGDG
                     AALECALVQGISRFRDPGRGQGLAGIKRYLERWEGKISIRSGSARLAIVPAWDEDVPL
                     REHLAPFPGAQVQIVIPAAQGKA"
     gene            complement(7201..>7491)
                     /locus_tag="VFU45_07735"
     CDS             complement(7201..>7491)
                     /locus_tag="VFU45_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5358994.1"
                     /translation="HGSQVMGDGSQVASPTTRDPRPATTITTALLPPPTVETLDPTGC
                     GDVFGAACCARLLAGDTFEQALVHANRMGARNAAFRGATALNAHLRGELVPA"
BASE COUNT         1066 a         2597 c         2698 g         1130 t
ORIGIN      
        1 tctggcagaa ccggatcacg tcgtccggct tcagggcgtc gatctcgagg tcgaagacgt
       61 cgaggtccgc gaacgccttg aacaggaccc ccttgccctc catcaccggc ttgccggcca
      121 gcggaccgat gttgccgagc cacagcaccg cggtgccgtt ggtcaccacc gccacgaggt
      181 tgccccgcgc ggtgtagcgg taggcgtcct ccggccggtc cttgatggcc aggcacggct
      241 cggccacccc cggcgtgtag gcgagcgaca ggtcgcgctg gttctggaag cgcttggtag
      301 gaacgacctc gatcttgccg gggcgccctt cttcatggta gcggagcgcg tcctcgcgct
      361 tgcggtcggc catcgctacc gcgccgggac gaggtcgggg cggaaatggt acaggaagag
      421 cacggtggag acatcgcgcg ccgtcggcac cgggacgagc gggttcgacg acatcaggtc
      481 gtcgagcgaa tcgctgtgct ggaagagccc catgctgtgc cccagctcgt gcgccaccgt
      541 gagcccgagg caccacgtgg tgctgtcctg gcccagcggg tacttgttct cgacgtagat
      601 ccggatcggc aggaggagtt tcctgtggtc ggggcggctc accaccacgt cggtggcgcc
      661 gctgcactgc ggggccatcg accgcatccg caccgccgcg gggaggaccg gcggcacccc
      721 ggcatagacg agtacgtcgg cgttggtgga atcactcacg agcgtcgcgt catactcccc
      781 gtactgcagc acctgcttcc agagcgcgat gccgtcccgt acgtggcccg ggaggccgag
      841 accgccgctc gtgtccgccc agatccggac cggcagccgc gacgtgggcc agtggaacca
      901 gacggtgtcg gtgtagaccg agtcctggtg gtacacgacg ccgttcacgg tatcgtccgc
      961 ggtcatcgag tcccgcgcca cgagggtgaa cccatacccc gccggccgga gcgggctcga
     1021 gagctcggcg caggcgacgg ccacgccggc ggcggccacg gccagggcca gcaggcctcg
     1081 ccgcctcaat gcgccactcc gacggccagc gtgatccgat gcacgtcctc gctcccggtg
     1141 tagccctgcg actggaggtg ggtcgtggtg aagcgatggt aatcccagcg tagcgcggcc
     1201 gagagatccc accccggccg ccaggcgcgg cgatactcga ggcccagccc gccggccgcg
     1261 cgggtgggcg caccgtcctg gaagatcccg gtcttctggg ccgggaggta cgtgatgaag
     1321 ccgagcgccg cccgccaccg cagccggggt gccacctggc cctcggcgcc gaacgtgagc
     1381 gtagcggtcc tgagcgtgcc gaggtccgtc gtgacgccgt tctccttcga ggcgtaggag
     1441 ccgctcgcca ggccgagctc gatgtcgaac cggcggccct tcgcatcgag cggaatggac
     1501 ccccccagca gcaataccgg tgccaccgcc ggggtgatgg tgagcggcga cacgatcgca
     1561 tcgctcacga tcttggtcga cgccaccact ccgatccggc ccgtccactg tacctgtgcg
     1621 gccagcaccc ccggcgccag ggcgatggcg aatgccggcc cggccgccag gcgccgaagc
     1681 gcggggtgaa ggatgttcaa catgagaggg aggaagatag ccgggtcggg gatggtgccg
     1741 ggggatgttc gggggagcga ggtgggaagg gaaaagtgaa aagggaagag tgaaaagtga
     1801 aaagggaaga gtgaaaaggg gaaaagggaa gagggaagag agaagagaga agcgggaaaa
     1861 aaggggctcg gtgccgtgtc gttcgtcgtt cctacctctg acgtctgacc tcgcaccgct
     1921 cccctcccac cttttccctc ttccctgttc cctcttccct cttccctgtt ccctcttccc
     1981 tcttccctgt tccctcttcc ctcttccctc tcacctctca cctctcacct cttaccgaca
     2041 taccctcgca gccgcgccag catcttccgg tcgttggtga ccatgtcctc gctcttgggg
     2101 gtctcgagga tcttggccac cccggccatc cgcgggtcgt tcatgatgcg cttgaacggg
     2161 cccgcaccga gggagccctc cgcgatcagc tcgtggcggt cgcgatggga agcgaacggc
     2221 gtcttggagt cgttgaggtg gaggcaccgg aggcgttcga ggccgcacac ccggtcgaag
     2281 cgctcccaca cgccgtcgta gtcgttcacc aggtcgtagc ccgccgcgta caggtggcag
     2341 gtgtcggcgc agtagccgac ccgcgcctgc accgccttgg gcatgcgggc ccggagctcg
     2401 gccagctcct cgaaggtgct gccgagggcg gtgccggtgc cggccgtcgt ttccatgagc
     2461 accatcaccg gcccgggcac cgcctccagg cactcggcgt aggcctgggc gttgcgggtc
     2521 aggccggcgt cgcggtcgtc catgaagttg ccggggtggg acaccacgta cggcacgccg
     2581 agctgggtgc agcgggtgag ttccgcggtg aacgacgcga tcgatcgctt gcgcagcgcc
     2641 gcatcggggg acgcgaggtt gatgaggtag ctgtcgtgcg acaccgccac gccgagaccg
     2701 ctctccgccc aggcggtgcg gtaggccgcg accgtcgcct cggagagcac gggctcccgc
     2761 cattggttcg gcgtcttggt gaagagctgg atggccgtgg cgccgatctc gacgccgcgg
     2821 ggcggcgccg tctgggggcc gccggccgtg gacacatgcg cgccgaggag ctcggccgcc
     2881 gtcactttct tcgttgcagc cattcggtgg ggtcgagggc gacgccgttc tcgccgcgga
     2941 tctcgaagta gaggtggggg ccttcgtcgg tgttggcgcc gccggaggtg ccgatcatct
     3001 gcccgcgcac caccgcctgg cccttggtga ccgacgaggt cgaaagctgg gagtagatcg
     3061 agtagtagcc gccgccgtgg ctcaggacca ccgagagccc gtaggtgccc agcggctgga
     3121 ccatctccac ggtgcctgcc tcgaccgcct tcaccggggt gccggggggt gccgcgatgg
     3181 cgatgccgtt gtgcttgatg acggccccgg agttgagctc gtcggtgccg aacccgtaga
     3241 gcaccgtgcc gtccatcggc cagccgagct tgccgatgct ggccgtcgtg atggacgagc
     3301 ggggcggcgc cacccggcgg cccgacgcgc gggcggcggc ggcgcgggcg gcggcctcgc
     3361 ggcgggcgcg ctcgagggtg gcgatgagcg cgttcatcgc ctcctcgtcc cgctggagcg
     3421 ccgagagccg gctctcggtg gaccgggtgt cgcgccgcac gtcccggagg cgcgagcgct
     3481 gctcgtcggc caggtcgccg tagtgctgga gctcctggtc gcgctcctcg cgggaggtgg
     3541 tgaggctcgc gcgatagttg agcaggtcgt cgcgctggcg gttgacccgg tcgcgcaggc
     3601 gctccacgtc ggagaccagc tggcggtcct gccggctgcc gaggtagagg tacttgaagc
     3661 ggctcaccag gtcaccgaag gattccgccg ccaggagggc ctggaaatcg tagagggggc
     3721 cgcgcttgta gatctcgacc aagcgccggt agagcaccgc gcgcttgtcg gccaggttgt
     3781 cctgcgccag cgcgaggctg gcgctcacct ggtccagctc ctggttgagc ccgccgatct
     3841 ggcggtcgag ttcgttgacg atgcggttgg tggcgtcgcg ctggcgggtg atgttgacca
     3901 gctcggactc ggcgtcgtgg gcctggccct gcaggtcgct ccgctgctgc tgcagccgct
     3961 cccgctcggc gcggatctgc tccaggcgct tccgggagtc ctgcatccgc tgctggatgg
     4021 tgccctgggc ggagaggtgc cggggtgcga gggcgcagag cgccagcagg gtcaggaagg
     4081 ccgccctgcg ccatgccccg cgtccctccg tccctgcgcc cctgcgccta gacatccttg
     4141 agatgccggc ccacgctgaa caggctcccg ccgaagccga tggcggtgcc gaagagcacg
     4201 atggcgccga ggtagtcggg ctcgaagaag acgagtccgc tgaagaggcc ggcgttcccc
     4261 tggacggcgc tgaaggcgag ccagcagagc ccgatggcca ggagcccgcc caggagcccc
     4321 ttgaccgcgc cctcgagcag gaaggggccg cgcacgaacc agttggaggc gcccaccagg
     4381 cgcatgatgc cgatctcgcg gccgcgctgg agcaccgcca tccggatcgt ggtgccgatg
     4441 atgaccaccg ccacgatggc gaacgcgagc ccgatcacga agcccaccag ccccgcgatg
     4501 tcccggatcc ggtcgagctt ggcgatccag tcgcgcccga agcgcacgtc gtcgacgaac
     4561 tggtagccct tgatccgctc ggccacgcgc gtcaccgccg cctcgtcgcg gaagccgtcc
     4621 ttgagcgtca cctcgagcga ggcggggagc gggttggcct ggaggtcgcg gtaggcgtcg
     4681 cggaactcgg tgagctcccg tcgcgcccgc gcgagcgcca cgtcctcgct cacgtaggtg
     4741 acctgctgca cctccggata cgccgcgatg tcggtcgagg cctgggcgat gacctcggcc
     4801 ggcgtgcccc ggaggacgta cgccacgatc tcgacccgct cctcgacgcc cttgagcgcc
     4861 tggcggaggt tgaccgccac cagcccgaag agccccacca cgaagaggct gaatccgatg
     4921 gtggtgaccg acagcatcga caggagcggc gtccgccgga aggagaggag cgcctcgcgc
     4981 agcaggagcc tcatcggccg ccctccaggt cctcggccgc ggggatccgc tcctcgccgc
     5041 tgtcgtacac caccatcccc tcccgcatct cgatggtgcg gtagggcgcc gacttgacca
     5101 ggtcgaggtc gtgggtggcc atcaccacgc aggtgccgcc ggcgttgatg tcgcgcagga
     5161 gctggaagac gccgcgggtg gcgcgctcgt cgaggttgcc ggtgggctcg tcggccagga
     5221 ggatggcggg gtcgttcacc agcgcgcgcg cgatggccac ccgctgctgc tcgccgcccg
     5281 agagctcgcg cgggtacgcc tgtcccttgg ccgcgagccc cacctgcgtc agcacccgca
     5341 tgacccggcc ggggatggta tcgcgcttgg ccccggtcac ctcgagcgca aaggccacgt
     5401 tctcgtgcgc ggtgcggtcc tcgaggagac ggaagtcctg gaacaccacg cccagccggc
     5461 ggcggagctt gggcacgtcg ccgcgggaga gggtgccgat gttgaagccc gacacccgca
     5521 cctcgccctc ggtggggcgc tgctcggcga acatcagctt gaggatggtg gacttgccgg
     5581 cgccggaatg gccggtgagg aagacgaact cgcccttgtg gacccggagc gagacgtcac
     5641 ggaggccgaa gtggcccttg gggtacgcct tgaagacgct ggtgaactgg atcacgcccg
     5701 aatgatagga accgcggagg ggggtgtcta gcgcctccgc gccccggccg ggcccgcgct
     5761 cacgccacca tcacggcgcc gtacgccccg ccgttggcca tcaccacccc caggaggcag
     5821 agcacctgga gcgcgtcggc cacccgctcg gtgcgccagc ccagctcccg ggcgagcgcg
     5881 ccgccgtcga gcgggccgcg gtcgatcagg aggtcgaagg cggcgcggct gtcgttgccg
     5941 aagtggccca ggagccgggg ccgggtggag tcgcgggtga tggcctgcac gccgagctgg
     6001 tggtgcgaga gcacatggtc gatggcctcg gcctggtcct cggtgagccc ggtgagcacc
     6061 acgtagcgct cgtcggtcac gtccatccgc tgcagcagct tggccaccac ctcgtcggcg
     6121 caggagaggt cgacgaggtc gagctcggag aagtcgagga gcgccacccg cgcgccgcgg
     6181 agcgtcatct cgcgctcgat cgagttccgc accgccgccc cggtggggcg ggtcaccagg
     6241 ttcttgggcg cccgggcggg gtgcgcctcc tggagcagcg tctcgagacg gatcgtggtg
     6301 accatcaggc cttcccctgg gcggcgggaa tgacgatctg gacctgggcg ccggggaagg
     6361 gggcgaggtg ctcgcggagc gggacgtcct cgtcccacgc cggcacgatc gcgagtcggg
     6421 cgctgccgct gcggatgctg atcttcccct cccaccgttc gaggtaccgc ttgatcccgg
     6481 ccagtccctg cccgcgaccc gggtcgcgga accggctgat gccctgcacc agcgcgcatt
     6541 cgagcgcggc gccgtccccc cagcgctccc cgatccgctt ggcctgcgtc gcttcgaggc
     6601 tcttgcggaa gcccaccccg ccgtcggcca cggcgatgac caccacccgg cggcccaggc
     6661 gcttccgcca gttgtatgcc tggatcgcca cccagccggt ggtcccggcg tgctccacaa
     6721 tattctgaca tgcctcgctg agcgccatag cgaaccccat caccgccttg gcctcgagcc
     6781 ccagctcgcc ggtcacgatc ctcgccgagc ggtcctggac ccgctccact acggcgtgga
     6841 cgtcctccga cgcccggacc ggcgtcacgt cgagcaggac gtcgctctcc tcctgggggc
     6901 gggcccgggg caccttgccg tggagctcga agagctcggc cgcctcgcgg aagaacccga
     6961 tccgcgtcca gtagctccgg gcctgctcgt cctccggcac cgtgaagagc ggcctggggc
     7021 ccccggcctg cgccaccgcc tggccggcga tgagcattcc cgtgaagccg tagggcgacg
     7081 accacttcga cccccgcgcg tcgaagagca tcttgtcggt cgcgggcccc gccgcgaagc
     7141 tcgcggcgaa ctgctcgaac gtgcggtcgt ccaggtgcga ggggatctcg acgatgcggg
     7201 tcacgcgggc accagctccc cccggaggtg ggcgttgagc gcggtcgccc cgcggaaggc
     7261 ggcgttcctg gcccccatcc ggttggcgtg gaccagcgcc tgctcgaagg tgtcgccggc
     7321 caggagccgg gcgcagcacg ccgcgccgaa gacgtcgccg catcccgtcg ggtcgagggt
     7381 ctcgacggtc gggggcggga ggagcgcggt ggtgattgtg gtcgcgggtc gcgggtcgcg
     7441 ggtcgtgggg cttgcgacct gcgacccgtc gcccatgacc tgcgacccgt g
//
LOCUS       DASUAP010000289         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_786273, whole genome shotgun sequence.
ACCESSION   DASUAP010000289 DASUAP010000000
VERSION     DASUAP010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786273"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1092)
                     /locus_tag="VFU45_07740"
     CDS             complement(238..1092)
                     /locus_tag="VFU45_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5358995.1"
                     /translation="MATPTLTSHTLPGALGPILVDVRAAARAEGRPAVVLLPGFKGFK
                     DWAFFPPLAERLARAGLAAVTVSVSGSGVDAAGEFTELDRFGHNTYSAELDDLRTVLA
                     ALAAGGLGTAAPHAIGLFGHSRGGGIAVLHAARDPRIRGLVTWGAISSVDRWNTATKD
                     DWRSRGRIDVVNSRTQQVLPLYIDLLEEVEQQGEGRLNIIGAASSISVPWLLLHGEDD
                     ETVEPSEAERLAEAAPQASRRLMMVRNGTHTFGTTHPWDGPTAEYDLAASATIDWLGK
                     ALAGAGDK"
     gene            1210..1767
                     /locus_tag="VFU45_07745"
     CDS             1210..1767
                     /locus_tag="VFU45_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358996.1"
                     /translation="MRASHPALLATGRTLAELRDSLVRQWADWLDDRTSASPAIPRPL
                     LERELQLLLDVLTELVGPLRREATELWSRTCEHYGRSAAARGLAAGEVVEEIQFLREL
                     LIRNLAGTLATMRPRQAMAIMLRLNQVIDRGIGVAVAGYTDALVAQLFAHDGVPSAET
                     ALDSAEVERQLGDIEKQLGELVARN"
     gene            1878..3014
                     /locus_tag="VFU45_07750"
     CDS             1878..3014
                     /locus_tag="VFU45_07750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5358997.1"
                     /translation="MQLPPIAALPWLVPFIPLIRLAFRGRRPALADAPEAAGTLVSVV
                     IPARNESGNIEPVIRSVLASRYAPIEVIVADDRSTDDTAVIVERIAREDPRVRLIRGA
                     DLPPGWYGKPWACLQGFRAASGTFVLFTDADTRHEPALLGHAVGALERERADLVTILP
                     TQVCLTLGERLVMPQLFSLLGAQYPPHRVNHARTAADVIANGQFILVRRDAYLEVGTH
                     EAVRHEVAEDLALGQEFWRRGKKLHLAHAEELMRTRMYTSLAQLVEGWSKNLYLGARR
                     AYADDPLRRSLAPFGVLLGFLFWLLPPLALAASFAWPALAGPAAWACGFSALFWMLVT
                     AGMEIPFAYGLLYPAGAALAAWIALRSIVRGRTKVEWKGRVYGA"
BASE COUNT          459 a         1157 c         1106 g          467 t
ORIGIN      
        1 gggccggcac cgacatccgc cgctcgacct tccggctctt gcagccgggg cactcgggca
       61 cgtcctcgcc gaggaccagg aattcgaatt cccggctgca cgcggggcag tggtagtcgt
      121 agaggggcat ggacggaata gtaaaaaggg aaaagggaaa aggggaacgc gggcgggaag
      181 cgtaagcacg gaaagcgagg gaaggcgacg gaaagcgggg gaaagcgtag gaaggcgtca
      241 cttgtcccct gctccggcca gcgccttccc cagccagtcg atggtggccg aggccgcgag
      301 gtcgtattcc gccgtcgggc cgtcccacgg atgcgtggtg ccgaaggtgt gggtgccgtt
      361 ccgcaccatc atcagccggc gcgaggcctg gggggccgcc tcggccagcc gctccgcctc
      421 ggaaggttcc acggtctcgt cgtcctcgcc gtggaggagg agccacggga ccgagatgct
      481 ggaggccgcg ccgatgatgt tgagccgacc ctctccctgc tgctccacct cttccagcag
      541 gtcaatgtag agcgggagca cctgctgggt acggctgttg acgacgtcga tccggccccg
      601 ggagcgccag tcgtccttgg tggcggtgtt ccagcggtcc acgctgctga tggcgcccca
      661 ggtgacgagc ccgcggatgc gcgggtcgcg cgcagcgtgc agcaccgcga tcccgccgcc
      721 gcgggaatgg ccgaagagcc cgatggcgtg gggcgcggcc gtgccgagcc cgccggcggc
      781 aagtgccgcg aggaccgtgc gcaggtcgtc gagctcggcg gagtaggtgt tatgcccgaa
      841 gcggtcgagc tcggtgaact cccccgcggc gtccacgccc gagccgctca cgctcaccgt
      901 gacggccgcg agccccgccc gcgccaggcg ctcggcgagc ggcgggaaga acgcccagtc
      961 cttgaacccc ttgaaccccg gcaggaggac gaccgcgggc cggccttccg cgcgggcggc
     1021 ggcccggacg tcgaccagga tgggtccgag cgcgccggga agggtgtggg aggtgagcgt
     1081 gggcgtggcc attgtgcgcg aaggtaatcc gctgcgccgc ggaatcaacg acgcccttgg
     1141 cacccgctac tggccccctg ccccggccgg ccctaccttt cgtccgcaca tcacccggga
     1201 gccccacacg tgcgcgcgtc gcatcccgca ctgctggcca ccggccgcac gctggccgaa
     1261 ctccgtgact ccctcgtccg tcaatgggcc gactggctcg acgaccggac ctccgcctcc
     1321 ccagccatcc cgcgcccgct gctcgagcgg gaacttcagc tcctcctcga cgtgctcacc
     1381 gaactggtgg ggcccctccg gcgcgaggcg accgagctct ggtcgcgcac ctgcgaacac
     1441 tatggccgct cggccgcggc ccgcgggctc gccgccggcg aggtggtgga ggagatccag
     1501 ttcctccgtg agctcctcat ccggaacctg gccggcaccc tcgccacgat gcggccacgc
     1561 caggcgatgg ccatcatgct ccggctcaac caggtgatcg accgcggcat cggcgtggcc
     1621 gtggcgggat acaccgacgc gctggtggcg cagctgttcg cccacgacgg cgtgcccagc
     1681 gccgagacgg ccctcgactc cgccgaggtc gagcgccagc tgggcgacat cgagaagcag
     1741 ctgggggagc tggtggcgcg taactgacgc tatcagctat cagctatcag ctatcagcgc
     1801 ttcagggatg ccgtaatgcg gcacgaacgc aagtaccctg gaccgtcgac atcgacggat
     1861 gatgcttagc gacctccgtg caactgcccc ccatcgccgc gctcccctgg ctcgtcccct
     1921 tcatcccgct catccgcctg gcgttccgcg gccggcgacc ggccctcgct gacgcgccgg
     1981 aggcggcggg cacgctcgtg agcgttgtca tccctgcgcg gaacgagtcc ggcaatatcg
     2041 agccggtgat ccgctccgtg ctcgcatccc gctacgcccc catcgaagtg atcgtggccg
     2101 acgatcgctc caccgacgac accgccgtca tcgtcgagcg gatcgcccgg gaggaccccc
     2161 gcgtgcgact gatccgcggc gccgacctcc cgcccgggtg gtacggcaag ccttgggcgt
     2221 gcctgcaggg attccgcgcc gcgagcggca ccttcgtcct cttcaccgac gccgacaccc
     2281 ggcacgaacc cgccctgctc ggccacgcgg tcggcgccct ggagcgggag cgggccgacc
     2341 tcgtcacgat cctccccacc caggtctgcc tcaccctcgg ggagcggctg gtgatgccgc
     2401 agctcttttc cctgctcggc gcacagtacc ctccccaccg ggtcaaccac gcccgcaccg
     2461 cggccgacgt gatcgccaac ggccagttca tcctcgtgcg gcgcgacgcc tacctcgagg
     2521 tcgggaccca cgaggcggtg cgacacgagg tggccgagga cctggcgctg gggcaggagt
     2581 tctggcggcg ggggaagaag ctccacctgg cgcacgccga ggagctgatg cggacccgga
     2641 tgtacacaag cctggcacag ctggtggagg ggtggtcgaa gaacctctat ctcggcgccc
     2701 gccgggccta cgccgacgac cccctcagac ggagcttggc gccattcggc gtgctgctcg
     2761 gcttcctctt ctggctgctg ccgccgctgg cgctcgcggc atcgttcgcc tggccggcgc
     2821 tggccggtcc ggccgcatgg gcgtgcgggt tctccgccct cttctggatg ctcgtgaccg
     2881 ccgggatgga gatcccgttc gcctacgggc tcctctatcc ggcgggagcg gcgctcgccg
     2941 cctggatcgc gctccggagc atcgtgcgcg ggcggaccaa ggtggagtgg aaggggcggg
     3001 tgtacggcgc gtaaggtggg cggacagggc gacaccgatg tgactccccc gctccctgcc
     3061 cgctgttgac ccgcttgtca cccgcttccc ttcaccgcct cgatcccggc gagcgagaac
     3121 gcgatgatgt gctccgccat cgctgccgcg gattccggag tgcgcggaat ggccggcgtc
     3181 acccggtcc
//
LOCUS       DASUAP010000290         7631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1331062, whole genome shotgun sequence.
ACCESSION   DASUAP010000290 DASUAP010000000
VERSION     DASUAP010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331062"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFU45_07755"
     CDS             complement(<1..305)
                     /locus_tag="VFU45_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358998.1"
                     /translation="MSIPGVVLERDGAQYRVLLGGGSEVRAVLRGKLKRDTPQVVVGD
                     RIQLEDAPQGELYGIAAVEPRRTIVERRVPLGRGARPVVANVDRVIVVTASIDPAPIP
                     "
     gene            complement(302..616)
                     /locus_tag="VFU45_07760"
     CDS             complement(302..616)
                     /locus_tag="VFU45_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5358999.1"
                     /translation="MSEATLGGYMAAHGRAAAFGGSDGQAYSVGIWADEVPDERGRFG
                     AALVFVRWDSGGERPVGHVETDYLAWGATAAEAEERVKALSLFDVKQALDEAIAARPE
                     GW"
     gene            complement(613..1542)
                     /locus_tag="VFU45_07765"
     CDS             complement(613..1542)
                     /locus_tag="VFU45_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU5359000.1"
                     /translation="MRSTLLWLSQRQGIFNFVRTNRLAKKFASRFVAGETVDTAVAAV
                     RELNGAGITASLDLLGESVARPDEAAHARDAYLGMLDTIATAGVRANVSVKLTQMGLD
                     ISPDVCRANMRAILDKAKALDSFVRLDMEGSAYTDRTLSFFYDQLYADYAKHCGVVIQ
                     SMLRRSPVDIDKLIGLKVRVRLCKGAYLESPEVAFPDKKDVDAQYVSLMERLLVDGNY
                     PGIATHDERIIEHAKGFARGKGIAPERFEFQMLYGVRRDLQLRLRQEGWNLRVYIPFG
                     TQWYPYLMRRLAERPANIAFILGNVIRETLPGR"
     gene            1699..2820
                     /gene="rho"
                     /locus_tag="VFU45_07770"
     CDS             1699..2820
                     /gene="rho"
                     /locus_tag="VFU45_07770"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU5359001.1"
                     /translation="MPVSGVLELQPDGNGALRRREAGYLPDPRDVSVPARLIRSAGLR
                     AGDEVTGETGRAERGRAAPLASVTTIFGRPAASVAERPAFERLTAVHPRQRLRLACGR
                     RVGGQPDLTGRAIDLIAPLGRGQRATIVAPAKAGKTTVLTAVAEGIKANHPECRLLVL
                     LVDERPEEVTEMESADVGEVVASSFDCPADQHVALAELTIERARRLVELGEHVVIVLD
                     SITRLARAFNAVETKSGGRTMSGGLGAGALERPKRLLGSARATPDGGSLTIVATSLID
                     TGSKMDQVIFEEFKGTGNCEVTLSRELAERRLYPAIDVTASGTRREELLLSPQELDVA
                     RALRKRLADLQPVPASQLFLELLRRNADDAALAKALGVG"
     gene            complement(2907..5021)
                     /gene="fadJ"
                     /locus_tag="VFU45_07775"
     CDS             complement(2907..5021)
                     /gene="fadJ"
                     /locus_tag="VFU45_07775"
                     /EC_number="1.1.1.35"
                     /EC_number="4.2.1.17"
                     /EC_number="5.1.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF008363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HEU5359002.1"
                     /translation="MPVFSVDTRDGVAVLTLDVPGQPVNTLNADVQREIDELLPRLAA
                     DAAVRSVVLISGKPDNFVAGADIEQFTRLTSAEEGAALSRGGQEMAQRLQDFPKPIVV
                     AIHGACLGGGLELSLACTWRVISDHPKTQLGLPEIQLGIVPGAGGCERLPRLIGLRAA
                     LDIILAGKSERAAKAFKLGMADELVHPAILRDTAVAAARRLADRVPLRAPRPSGLMAW
                     FLDRTRWGRALVFRGARAQVLRKTGGNYPAPLKAIDVIRTGYDQGLAAGFAAEHQAFG
                     DLAVTDVSRNLVRLFFADTALKKDDFGTGGARPREVRRLGVIGSGFMGAAIAGTAVSN
                     AAVEVRLKDAELARVGRGLKAATGLLDERLKRRRITRPEHQRLTALLTGAEDFRGFRH
                     VDLVIEAVFEDLTVKRQVLKEVEAAAPDAVFATNTSTIPIGDIGADAARPERVIGMHF
                     FSPVDKMPLLEVIPSAHTGPEAIATAVAFGRRMGKKVVVVQDRPGFWVNRILTPYLNE
                     AGRLLQEGTPIKEIDAALTRFGFPVGPIALIDEVGIDVAQKAGKVMHAAFGERLEPSG
                     VVGTMIADKRLGKKNGRGFYVYHDGHKTGPDESVYDLLGVHPSSKYAEDIERRLVCAM
                     LNEAALAFAEGVVRSPRDGDLASVFGIGFPPFRGGPLRLIDTWGAAKVVEMLSDLASH
                     AGPRFTPAPALVEMARSGKTYY"
     gene            complement(5021..6316)
                     /locus_tag="VFU45_07780"
     CDS             complement(5021..6316)
                     /locus_tag="VFU45_07780"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5359003.1"
                     /translation="MLSIPLDRRCALIAGLRTPFARSGTVFRDVPATTLARAAARELL
                     YRAELDGREVDQVIFGQVIQSVLAPNVAREVSLLPQFPRTIPAYSLNRACASSAQAVA
                     DAHDQIVQGNADVILAGGVESLSDVPILHSNGMRRILMDASKAKTLGQRLATFARIRP
                     RDLVPVTPAIAEPSTGETMGQSAEKMAKENGITRRAQDECALMSHQRAAAATANGRLP
                     AEIAPWFGGRGMDEILHADNGIRTDTSLEALSALKPVFDRRYGTVTAGNSSPLTDGAS
                     AVILMADAKARSLGYRPLAYVRSYAVAAVDPGWQLLMAPAVAVPLALKRAGLSWKDIG
                     VLEIHEAFASQVLSNVQAWGSKKWADFFGLPAPTGEVNWDITNVNGGSIAIGHPFGAT
                     GTRLVTQLANEMVRRDAQFGMLSICAQGGMGFAMILERA"
     gene            complement(6385..6948)
                     /locus_tag="VFU45_07785"
     CDS             complement(6385..6948)
                     /locus_tag="VFU45_07785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5359004.1"
                     /translation="MSEPRLSPPALPALPRPSTWFTWHGHLIRPGVRVLDLACGEGRH
                     AVAAAALGAEVVAVDREATRIDVAREAAAAQGLTLDLRTVDLEGEWPELGTFGVVLAF
                     NYLDRARMADVIARVAPGGVLVMETFLVAQRQLGWGPKSDAYLLNPGELARLVAPLEI
                     VHGREVYEPVDTDRWRAVAGIVAQRRI"
     gene            6988..>7631
                     /locus_tag="VFU45_07790"
     CDS             6988..>7631
                     /locus_tag="VFU45_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5359005.1"
                     /translation="MLVIGGGIVGAAVARDAAMRGYRVALVEQGDFGAGTSSHSSRLA
                     HGGLRYLEHGEFKLVFEALRERGILLRIAPHLVRPLPFIFPVHHGDRVGFWRLAAGMW
                     LYDLLAMFRNVPRHRMLGKRGLLRAEPMLRERGLVGGARYFDAQLDDARLTLATVRSA
                     ARHGAVVASHATVSGLEVAGGRVSGAHVSLGSGPPVLVRATVVVNATGPWCDRIR"
BASE COUNT         1073 a         2704 c         2795 g         1059 t
ORIGIN      
        1 gggatcggcg cgggatcgat gctggcggtg acgacaatga cgcggtcgac gttcgcgacg
       61 accgggcggg cgccgcggcc gagcggcacc cgtcgttcga cgatggtgcg cctcggctcc
      121 accgcggcga tgccgtagag ctccccctgc ggcgcgtcct cgagctggat ccggtcaccg
      181 acgaccacct gcggcgtgtc ccgcttgagc ttgccgcgca gcaccgcccg gacctcggag
      241 ccgccaccga gcaacacgcg gtactgggcg ccgtcgcgtt cgaggacgac gccggggatg
      301 ctcaccaccc ctccgggcgg gcggcgatgg cctcgtcgag ggcctgcttg acgtcgaaga
      361 gggagagcgc cttgacgcgc tcctccgcct cggccgcggt ggcaccccac gccaggtagt
      421 cggtctcgac gtggcccacc ggccgctcgc cgccggagtc ccagcggaca aaaacgagcg
      481 cggcgccgaa gcggccgcgc tcgtccggga cctcgtccgc ccagatgccc accgaatagg
      541 cctgaccgtc gctgccgccg aaggcggcgg cgcggccgtg ggcggccatg tagccgccga
      601 gggtggcttc gctcaccggc ccggaagggt ctcccggatc acgttgccca ggatgaaggc
      661 gatgttggcg ggccgttccg cgagccggcg catgaggtag ggataccact gggtgccgaa
      721 ggggatgtag acgcggaggt tccatccttc ctgccgcagc cggagctgaa gatcgcgccg
      781 gacgccgtag agcatctgga actcgaaccg ctccggcgca atgcccttgc cccgggcgaa
      841 ccccttggcg tgctcgatga tccgctcatc gtgggtggcg atgccggggt agttcccgtc
      901 gacgagcagg cgctccatca gcgacacgta ctgggcgtcg acgtccttct tgtcggggaa
      961 ggccacctcg ggggattcaa ggtaggcgcc cttgcagagc cgcacccgta ccttgaggcc
     1021 gatgagcttg tcgatgtcga ccggcgagcg gcggagcatc gactggatga ccacgccgca
     1081 gtgcttggcg tagtcggcat acagctggtc gtagaagaac gagagggtcc ggtcggtgta
     1141 ggccgacccc tccatatcga gccggacgaa ggaatcgagc gccttcgcct tgtcgaggat
     1201 ggcgcgcatg ttggcgcggc agacgtcggg cgagatgtcg aggcccatct gcgtcagctt
     1261 gacggagacg ttggcccgca cgccggcggt cgcgatagtg tcgagcatcc cgaggtacgc
     1321 gtcccgggcg tgtgccgcct cgtccgggcg ggccacgctt tcgcccagga ggtcgagcga
     1381 ggcggtgatg ccggcgccgt tcagctctcg caccgcggcg accgcggtgt cgacggtctc
     1441 cccggcgacg aagcgggacg cgaacttctt ggccagccgg ttggtgcgga cgaaattgaa
     1501 gatcccctgc cgctgggaca gccagaggag cgtggagcgc atcatatcga aggatccccg
     1561 gaagaacgga ccaccccgcc cgcgagcgcc cccaagctag cgcgggcctc cggcgccgac
     1621 aaggcgaacc gtgccccgaa tgggttagtt ttggcgggtc gcacggcctc ctcgaccatc
     1681 cctccctccc aggcacgcat gccagtctcc ggggtcctcg aactccagcc ggacggcaat
     1741 ggcgcgctcc gccgccgcga agcggggtac ctccccgacc cccgcgacgt cagcgtgccc
     1801 gcacggctca ttcgttcggc cggcctgcgg gccggcgacg aggtcaccgg cgagaccggg
     1861 cgcgccgagc gcggccgcgc cgccccgctc gcgtcggtga ccacgatctt cggacggccg
     1921 gcggcgtcgg tcgccgagcg cccggcgttc gaacggctca ccgcggtgca tccacggcag
     1981 cggctccgcc tcgcctgcgg tcgccgggtg ggcggccagc cggacctgac ggggcgcgcg
     2041 atcgacctca tcgcgccgtt gggccggggc cagcgcgcca ccatcgtggc cccggccaag
     2101 gcagggaaga ccacggtgct caccgcggtg gcggagggga tcaaggccaa ccacccggag
     2161 tgccggctcc tcgtgctgct ggtggacgag cgccccgagg aagtgaccga gatggagtcg
     2221 gccgacgtcg gggaggtcgt ggcctccagc ttcgactgcc ccgccgacca gcacgtggcg
     2281 ctggccgagc tcaccatcga gcgggcccgg cgcctggtgg agctcggcga gcacgtggtg
     2341 atcgtcctcg actcgatcac gcgactggcg cgggccttca acgcggtcga gaccaagagc
     2401 ggcggacgga cgatgtccgg cggcctcggc gccggggcgc tggagcgccc caagcggctg
     2461 ctcggcagcg cccgcgcgac gccggacggc gggtcgctca ccatcgtggc gacgtcgctg
     2521 atcgacaccg gcagcaagat ggaccaggtg atcttcgagg agttcaaggg gaccggcaac
     2581 tgcgaagtga cgctcagccg tgagctcgcc gagcggcggc tctacccggc catcgacgtg
     2641 acggcgagcg ggacccggcg ggaggagctg ctgctctcgc cgcaggagct cgacgtggcg
     2701 cgggccctcc gcaagcgcct ggccgacctc cagcccgtcc cggcgagcca gctcttcctc
     2761 gagctcctcc gccgcaacgc cgacgacgcc gcgctggcga aggcgctggg ggtggggtga
     2821 gggtcgcggg tcgcgggtcg cgggtcgcga gtcgcgggtc gcgagtcgac ccattcggaa
     2881 taagcactgc gcccgccacc cgctgcctag tagtacgtct tccctgacct ggccatctcc
     2941 acgagcgccg gcgcgggggt gaagcgcggc ccggcgtggc tcgcgaggtc ggagagcatc
     3001 tcgaccacct tcgcggcgcc ccaggtgtcg atcaaccgca ggggcccgcc gcggaagggc
     3061 gggaacccga tcccgaacac cgatgcgagg tcgccgtcgc ggggggagcg gaccactccc
     3121 tcggcgaagg cgagcgccgc ctcattgagc atggcgcaga cgaggcgacg ctcgatgtcc
     3181 tccgcgtact tcgacgacgg gtggacgccg agcaggtcgt agaccgattc gtcgggcccc
     3241 gtcttgtggc cgtcgtggta cacgtagaag ccccgcccgt tcttcttccc cagccgcttg
     3301 tcggcgatca tcgtccccac gacgcccgac ggctcgagcc gctcgccgaa tgcggcgtgc
     3361 atcaccttcc ccgccttctg cgccacgtcg atcccgacct cgtcgatgag cgcgatgggc
     3421 ccgaccggga acccgaaccg cgtcagcgcg gcatcgatct ccttgatcgg cgttccttcc
     3481 tgcagcaggc gcccggcctc attcaggtac ggcgtgagga tccggttgac ccagaacccc
     3541 ggccggtcct gcaccacgac gaccttcttc cccatccgcc ggccgaaggc gaccgccgtg
     3601 gcgatcgcct ccggcccggt gtgcgccgac gggatgacct cgaggagcgg catcttgtcg
     3661 acgggggaga agaagtgcat cccgatgacc cgctcgggcc gcgccgcgtc ggcgccgatg
     3721 tcgccgatgg ggatggtcga ggtgttggtg gcaaagacgg cgtcgggcgc cgcggcctcg
     3781 acctccttga gcacctggcg cttgacggtc aggtcctcga agaccgcctc gatgacgagg
     3841 tccacgtggc ggaacccgcg gaagtcctcc gcgccggtga ggagtgcggt caagcgctgg
     3901 tgttcggggc gcgtgatgcg ccgccgcttg agccgctcgt cgaggagccc ggtcgcggcc
     3961 ttgagccccc tccccacccg cgcgagctcg gcgtccttga gccgcacctc gaccgcggcg
     4021 ttgctcaccg ccgtccccgc gatggccgcc cccatgaatc ccgagccgat gacgccgagc
     4081 cggcggacct cgcggggccg cgcgccgccg gtgccgaagt cgtccttctt gagcgccgtg
     4141 tcggcaaaga agaggcgcac caggttccgc gacacgtcgg tgacggcaag gtcgccgaac
     4201 gcctggtgct ccgccgcgaa ccccgccgcc aggccctggt cgtacccggt gcggatgacg
     4261 tcgatggcct tgagcggcgc cgggtagttg ccgccggtct tccggaggac ctgggcacgg
     4321 gcgccccgga agaccagggc ccgcccccac cgggtccggt cgaggaacca cgccatgagt
     4381 cccgacgggc ggggggcgcg gagcggcacc cggtcggcga gccgccgagc cgcggccacc
     4441 gccgtatcgc gcaggatggc gggatgcacg agctcgtcgg ccatgccgag cttgaacgcc
     4501 ttggccgccc gctccgactt gccggccagg atgatgtcga gcgccgcccg gagcccgatc
     4561 aggcgcggta gccgctcgca gccgccggcg cccggaacga tgccgagctg gatctccggc
     4621 aggccgagct gggtcttggg gtggtccgag atgacgcgcc aggtgcacgc gagggagagc
     4681 tcgagcccgc cgccgaggca ggcgccgtgg atcgcgacga cgatgggctt gggaaaatcc
     4741 tgcagccgct gggccatctc ctggccgccg cgcgagagcg ccgcaccctc ctcggcgctc
     4801 gtgagccggg tgaactgctc gatgtcggcg ccggcgacga agttgtcggg cttccccgag
     4861 atgagcacca ctgagcgcac cgccgcatcg gccgcgaggc gggggaggag ttcgtcgatc
     4921 tcccgctgca cgtcggcgtt gagggtgttg accggctgcc ccggcacgtc gagggtcagc
     4981 acggccacgc cgtcccgggt gtcgacgctg aagaccggca tcaggcacgc tccaggatca
     5041 tcgcgaagcc catcccgccc tgcgcgcaga tcgagagcat cccgaactgg gcgtcgcggc
     5101 ggaccatctc gttggccagc tgggtcacga gacgggtgcc ggtcgcgccg aacgggtgcc
     5161 cgatggcgat cgacccgccg ttgacgttgg tgatgtccca gttgacctcg cccgtgggcg
     5221 cgggcaggcc gaagaagtcg gcccacttct tcgaacccca tgcctgcacg ttggagagca
     5281 cctgggaggc gaacgcctcg tggatctcga ggacgccgat gtccttccac gaaaggcccg
     5341 cgcgcttcag cgcgagcggc accgcgaccg ccggcgccat caggagctgc cagccgggat
     5401 cgaccgccgc gacggcgtag ctccggacgt aggccagtgg ccggtatccg agcgaccggg
     5461 ccttggcgtc ggccatgagg atcaccgccg acgcgccatc ggtgaggggc gaggagttgc
     5521 cggccgtcac ggtcccgtag cggcggtcga agaccggctt gagcgccgag agcgcctcga
     5581 gcgaggtgtc ggtgcggatc ccgttgtcgg cgtggaggat ctcgtccatc ccacggcccc
     5641 cgaaccacgg cgcgatctcg gccgggagac gcccgttggc ggtggcggcc gcggcgcgct
     5701 ggtggctcat cagcgcgcat tcgtcctgcg cccggcgggt gatcccgttc tccttggcca
     5761 tcttctcggc cgactggccc atcgtctcgc cggtcgacgg ctcggcgatc gccggggtga
     5821 cgggaacgag gtcgcgcggg cggatccggg cgaaggtcgc aagccgctgg ccgagggtct
     5881 tcgccttgct cgcgtccatc aggatgcggc gcatcccgtt cgagtgaagg atcggcacgt
     5941 cggagaggga ctcgacgccg ccggcgagga tcacgtcggc gttgccctgg acgatctggt
     6001 cgtgcgcatc ggccaccgcc tgggcggagg aggcgcaggc gcggttgagc gagtaggcgg
     6061 ggatggtgcg ggggaactgc ggcaggaggc tcacttcgcg cgcgacgttc ggcgcgagga
     6121 ccgactggat gacctgcccg aagatgacct ggtcgacctc gcggccgtcg agctcggcgc
     6181 ggtagagcag ctcacgggcg gcggcgcggg ccagggtggt ggcggggacg tcccggaaga
     6241 ccgtgccgct ccgtgcaaag ggcgtccgaa gccccgcgat cagcgcgcag cgacggtcca
     6301 gcgggatgga gagcatcgag ttcgcctctg cgccggtgcc gggttgcccc ggccggcggg
     6361 agcggagatt attggattcg ggcgctagat ccgtcgctgg gccacgatgc cggcgacggc
     6421 acgccagcgg tcggtgtcga ccggttcata cacttcccgt ccgtggacga tctcgagcgg
     6481 cgccaccagg cgggccaact cccccgggtt cagcaggtag gcatcggact tggggcccca
     6541 gccgagctgg cgctgcgcca ccaggaacgt ctccatcacg agcacgccac ccggcgccac
     6601 ccgcgcgatc acgtccgcca tccgcgcccg gtcaagatag ttgaacgcca ggaccacccc
     6661 aaacgtgccc agctccggcc actcaccctc gaggtcgacc gtgcgcagat cgagtgtcag
     6721 cccctgtgcc gcggccgcct cgcgggccac gtcgatccgg gtggcctccc gatcgacggc
     6781 caccacctct gcgccgaggg ccgccgccgc gaccgcgtgg cgcccctccc cgcaggccag
     6841 gtcgagcacg cggacgcccg ggcggatcag gtgaccatgc caggtgaacc aggtgctggg
     6901 ccgggggagg gcgggcagcg cgggggggga cagcctgggt tcgctcatct cgcctcgccg
     6961 gccgaactga cggcccggcc gtgggaagtg ctggtcatcg gtggcggcat cgtcggcgcc
     7021 gcggtcgcgc gggacgccgc gatgcgcggc taccgcgtgg cgctggtgga acagggcgac
     7081 ttcggggccg ggaccagctc ccactcgagc cgcctcgcgc acggcgggct ccggtacctg
     7141 gagcacgggg agttcaagct cgtcttcgag gcgctccgcg aacggggcat cctgctccgc
     7201 atcgcgccgc acctcgtccg tcccctgccc ttcatcttcc cggtccacca cggcgaccgc
     7261 gtcggcttct ggaggctcgc ggccgggatg tggctctacg acctcctcgc catgttccgg
     7321 aatgtgcccc ggcaccggat gctgggcaag cgcggcctcc tgcgggccga gccgatgctg
     7381 cgggagcgcg gcctggtggg cggcgcccgg tatttcgacg cgcagctcga cgatgcacga
     7441 ctgacgctgg ccaccgtccg gagcgcggcg cggcacggcg ccgtggtggc gagccacgcc
     7501 acggtgtccg ggctcgaggt ggccggcggc cgggtgagcg gcgcgcacgt ctccctcggc
     7561 agcggtcctc cggtgctggt ccgggccacg gtggtggtca acgccaccgg cccctggtgc
     7621 gaccggatcc g
//
LOCUS       DASUAP010000291         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_308919, whole genome shotgun sequence.
ACCESSION   DASUAP010000291 DASUAP010000000
VERSION     DASUAP010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308919"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFU45_07795"
     CDS             <1..591
                     /locus_tag="VFU45_07795"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5359006.1"
                     /translation="VVPPVLLPGDDLLELATLDAVAAVVRSCRKCPLCEGRTHAVPGE
                     GDPAARLVVVGEGPGQTEDQTGRPFVGRAGELLDKILLSIDVPRSSAYILNIVKCRPP
                     QNRAPLPDEIAACVPYLHRQLALLRPKVILAAGSTAAAGLLQSRKSLGQLRGQVHRYG
                     GVPLVVTYHPAALLRNPNWKKPTWDDVRIARQLLDA"
     gene            554..1978
                     /gene="dnaB"
                     /locus_tag="VFU45_07800"
     CDS             554..1978
                     /gene="dnaB"
                     /locus_tag="VFU45_07800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU5359007.1"
                     /translation="MTSVLPDNSSTPDAPAAPRGAPGDNDAEQAVLGAMLLDQDASLR
                     AAELLEDWMFYAERHRRIFRAMLHLAEQRIVIDQLTLRDELVRRGELDSTGGLPYASE
                     LADAVPTAANLEFHAGIVREKAIQRRLIEAATQIIQETYDGRSRSGELLDRAESKIFQ
                     IAQARRDDGFKGIKEMLWPTMERIEKLQGNAKAVTGVASGFRDLDQLTAGFQPSELII
                     VAARPSMGKTAFCLNLAAHAALEAETGVAIFSLEMSKESLVQRLLCAEAQVDSSKVRT
                     GRLRDDEYSRLARGAGVLSQARIWIDDTPSLTLLEMRSKARRLKAENDIGLIIVDYLQ
                     LMRSPEMAENRVQEISDISRSLKGLARELELPVIALSQLSRASEQRGGERTPILSDLR
                     DSGAIEQDADQVVFIHRPEMYKDLRDKAEKDGRNLDGVAEINLAKNRNGPTGSFELFF
                     NKAVTRFSDHSDREPPPEALPDDA"
     gene            1968..>2603
                     /locus_tag="VFU45_07805"
     CDS             1968..>2603
                     /locus_tag="VFU45_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5359008.1"
                     /translation="MTPKARSAYRCTECGHEHAKWVGRCEGCGAWNAVAEEPVAARRD
                     GGASGRGGARRAASGPGAVPARLGDIAESRLARWMVGLPEFDFVLGGGIVPGSLTLIG
                     GEPGIGKSTLLLQACARLEAAGRRTLYVSGEESPDQIRLRALRLEEDTRLVHVLGETR
                     LEAILESATTLAADVVILDSIQTVFTDALEGAPGSVGQVRECAALLMRFAKE"
BASE COUNT          391 a          919 c          938 g          355 t
ORIGIN      
        1 gtggtccccc ccgtgctcct cccgggagac gacctcctcg agctggcgac gctcgatgcg
       61 gtcgcggcag tggtgcgcag ctgccggaag tgcccgctct gtgaagggcg tacccacgcg
      121 gtgccggggg agggcgatcc cgccgcgcgc ctcgtggtcg tcggcgaggg gccgggccag
      181 accgaggatc agaccggccg cccgttcgtc ggccgggccg gcgagctgct cgacaagatc
      241 ctgctgtcca tagacgtccc gcgctccagc gcgtatatcc tcaacatcgt gaaatgccgg
      301 ccgccgcaga accgcgcgcc gctgcccgac gagatcgccg cttgcgtgcc gtacctgcac
      361 cggcagctgg cgctgctccg tccgaaggtg atccttgccg cgggatccac cgccgcggcc
      421 gggctgctgc agtcccgcaa gagcctgggc cagctgcgcg gccaggtcca tcgctacggc
      481 ggcgtgccgc tggtggtcac ctaccatccg gccgcgttgc tccgcaaccc caactggaag
      541 aagccgacct gggatgacgt ccgtattgcc cgacaactcc tcgacgcctg acgccccggc
      601 ggccccgcgg ggcgcgccgg gggacaacga cgcggaacag gcggtcctcg gggcgatgct
      661 cctcgaccag gacgcctcgc tccgcgccgc cgagctgctc gaggactgga tgttctacgc
      721 cgagcggcac cgccggatct tccgcgcgat gctgcacctg gccgagcagc ggatcgtcat
      781 cgaccagctg acgctgcgcg acgagctggt gcggcgcggg gagctcgaca gtacgggcgg
      841 cctgccctat gcctccgagc tcgccgatgc ggtcccgacc gcggccaacc tcgagttcca
      901 cgccgggatc gtgcgggaga aggcgatcca gcgccggctc atcgaggcgg ccacgcagat
      961 catccaggag acctacgacg gccgcagccg ttccggcgag ctcctcgacc gggcggagtc
     1021 gaagatcttc cagatcgccc aggcgcggcg ggacgacggc ttcaagggca tcaaggagat
     1081 gctctggccg acgatggagc gcatcgaaaa gctccagggc aacgcaaaag cggttaccgg
     1141 tgtggcgagc ggcttccgag atttggacca gctcacggcg ggcttccagc cgtcggagct
     1201 gatcatcgtc gcggcgcgtc cgtcgatggg gaagacggcc ttctgcctca acctcgcggc
     1261 ccacgccgcg ctggaggcgg agaccggcgt ggccatcttc tcgctggaaa tgagcaagga
     1321 gtcgctggtg cagcggctcc tctgcgccga ggcgcaggtg gacagctcga aggtgcgcac
     1381 cgggcggctc cgggacgacg agtactcgcg cctggcccgc ggggcggggg tgctgtcgca
     1441 ggcccggatc tggatcgacg acacgccatc gctgaccctg ctcgagatgc gctcgaaggc
     1501 ccggcggctc aaggccgaga acgacatcgg gctcatcatc gtggactacc tgcagctgat
     1561 gcggagcccg gagatggcgg agaaccgcgt gcaggagatc tcggacatct cgcggtccct
     1621 gaaggggctg gcgcgcgagc tcgagctccc ggtcatcgcg ctctcccagc tttcccgcgc
     1681 ctcggagcag cggggcgggg agcgcacgcc gatcctgtcc gacctgcgcg attccggcgc
     1741 catcgagcag gacgccgacc aggtggtgtt catccaccgg cccgagatgt acaaggacct
     1801 gcgcgacaag gccgagaagg acggccgcaa cctcgacggc gtggcggaga tcaacctggc
     1861 caagaaccgc aatggcccca ccggctcgtt cgagctcttc ttcaacaagg ccgtcacccg
     1921 cttcagcgac cattccgacc gggaaccgcc gccggaggcg ctccccgatg acgcctaagg
     1981 cgcggtcggc ctatcggtgc accgagtgcg gccacgagca cgccaagtgg gtgggccggt
     2041 gcgaagggtg cggggcgtgg aacgcggtgg ccgaggagcc ggtggcggcg cgccgggacg
     2101 ggggagcgtc ggggcgcggc ggagcccggc gcgcggcctc cggcccgggc gccgtgccgg
     2161 cacggctggg agacatcgcc gagtcgcggc tggcccggtg gatggtgggc ctgcccgagt
     2221 tcgacttcgt gctgggcggc gggatcgtgc cgggctcgct cacgctgatc ggcggcgagc
     2281 cgggcatcgg caagtccacg ctcctgctgc aggcctgcgc ccggctcgag gccgcggggc
     2341 gccgcaccct ctacgtgagc ggcgaggagt cgccggacca gatccggctc cgggcgctcc
     2401 gcctggaaga ggacacccgc ctggtccacg tcctcggcga gacgcgcctc gaggccatcc
     2461 tcgaatctgc caccacgctc gcggccgacg tcgtcatcct cgactccatc cagacggtct
     2521 tcaccgacgc gctcgaaggc gcccccggca gcgtgggtca ggtgcgcgag tgcgcggcgc
     2581 tcctgatgcg cttcgccaag gag
//
LOCUS       DASUAP010000292         5405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_717766, whole genome shotgun sequence.
ACCESSION   DASUAP010000292 DASUAP010000000
VERSION     DASUAP010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717766"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..735)
                     /locus_tag="VFU45_07810"
     CDS             complement(61..735)
                     /locus_tag="VFU45_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEU5359009.1"
                     /translation="MNPFTRLWRSTIGQKVLMALSGLIGVGFVIVHMAGNLQVFPAFG
                     GRDKLNGYSHLLHGPLGEVLWLARFVLLVAVVVHVLMAYRLTVRNAEARPIEYRKREP
                     QVSTWAARTLRWGGVLLLVFIPFHILNITTGDIHPGGAFVPGDVYANLTANFKVWWVT
                     AFYVLAMVALGLHLFHGAWSSWRTLGIAKPGPKPLHRDVAALVAIGVALGFALVPLSI
                     FFGWVR"
     gene            complement(873..1796)
                     /gene="mdh"
                     /locus_tag="VFU45_07815"
     CDS             complement(873..1796)
                     /gene="mdh"
                     /locus_tag="VFU45_07815"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU5359010.1"
                     /translation="MLQKVTVVGAGNVGATAAQRLAEKSLARTVVLLDVVDGVPQGKG
                     LDQWESAPIEGFDSRVVGTTSYEAAAGSDVFVVTAGIARKPGMSRDDLVKTNAGIVKS
                     VAENIKKVAPKSIVIVVSNPLDVMCYVMMRETGFPRERVIGMAGVLDTARYRSFLAEA
                     LDVSVEDIQAMVLGGHGDTMVPLISYTTVSGIPITQLLGKDKLDAIVTRARNGGAEIV
                     AFLKTGSAYYAPSAAAVQMVEAIALDKKRILPCSAWLTGEYGLKDVFCGVPCKLGARG
                     LESVLEVALTAEERAALHASAKAVTDVMAIV"
     gene            1961..2722
                     /locus_tag="VFU45_07820"
     CDS             1961..2722
                     /locus_tag="VFU45_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359011.1"
                     /translation="MTRSLCVLASVLALATPLAAQDNYEIQVYGSETVAPAVTMLELH
                     SNFTFDGGTTVVDGVRPTDHALHETVEVTHGFNDWFEVGFYFFTTAQDGGWHYVGSHI
                     RPRVRVPPGWGWPVGASISQEIGCQDRSYATEHCNYEFRPIVDKQMGRFYWAVNPSLE
                     LALTGPDRSIGFAPSAKVSFDFSRIVTGGFEYYGGWGPVAGLLPGDAREEGLYPTIDL
                     NVSPEWEVNAGVGIGLTPATDRLLAKLILGRRLGR"
     gene            complement(2719..3291)
                     /locus_tag="VFU45_07825"
     CDS             complement(2719..3291)
                     /locus_tag="VFU45_07825"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU5359012.1"
                     /translation="MLHIALLEPQIPPNTGNIARLCAATDTALHLVEPLGFSLDDKHL
                     KRAGLDYWDKVDLWRHPDWFEFRKAISRERCIYFSAHATRSYLEAPYQKNSVLVFGRE
                     DTGLPARIIEKHPEQCFKIPMPGDVRSLNLATAAGIVLYEALRQVGRGNEVPEGGMDA
                     DAGAAGAEAPAAVPGRGRTRRGGRGRSRTA"
     gene            complement(3404..4459)
                     /gene="moaA"
                     /locus_tag="VFU45_07830"
     CDS             complement(3404..4459)
                     /gene="moaA"
                     /locus_tag="VFU45_07830"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEU5359013.1"
                     /translation="MPDPLPLGSQASGHRPARPSDRRGRPIRSLRISVTDRCNLRCRY
                     CMPEEDYVWLPRESILSFEEITRLATVFASLGVERIRLTGGEPLMRRDLPTLVRLLRA
                     EVRPKDLALTTNGLLLRDEVAGLKAAGLERVTVSLDTLRPERAKEFSRTTRHADILDG
                     IRAAADAGFGIKLNVVVIRGFNDDELADLIAFGNEVGAEVRFIEYMDVGGATRWRMDD
                     VVPRDEIISRVGGAEPLPERGSAPAERYRSTDGTVFGIVASTTAPFCADCDRGRLTAD
                     GTFLTCLYAREGLDLRGPLRDGTSDGELTAIIAGRWSARDDRGAEDRLHAPDRGVLVP
                     LAGLRTDPHREMHTRGG"
     gene            complement(4452..4898)
                     /locus_tag="VFU45_07835"
     CDS             complement(4452..4898)
                     /locus_tag="VFU45_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU5359014.1"
                     /translation="MPFLTEHRLVVDDLAAEVAAPDRGAIVTFVGIVRDHHAGRAVRR
                     LDYSAYPEMAESECAAVVAEARRHWPVAVALRHRVGQLAIGDAAVAIVVAADHRDVAF
                     EACRFVIDEVKRRVPIWKKEYYADGSVAWVDPTAAGGTVPAKAPDA"
     gene            complement(4900..5163)
                     /locus_tag="VFU45_07840"
     CDS             complement(4900..5163)
                     /locus_tag="VFU45_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5359015.1"
                     /translation="MPQAKSITVTVRFLARYAEVVGRDMVSVTATVPATVDSILAQLG
                     ADVPSARALPPKPLCARNFRQVRGNEPLLDGDELALLPPLAGG"
     gene            complement(5228..>5405)
                     /locus_tag="VFU45_07845"
     CDS             complement(5228..>5405)
                     /locus_tag="VFU45_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU5359016.1"
                     /translation="VRGFGTFKIRRRKTRMARNPRTGDPVEVAARPVPVFKPSKELRA
                     LVAEEDAPSELPAT"
BASE COUNT          823 a         1903 c         1863 g          816 t
ORIGIN      
        1 cggtgctggt cccacttctt ctcgatgggc cccgcgggga ccctggcatt cagttccatc
       61 tcagcgcacc cagccgaaga agatcgagag cggcaccagc gcgaacccga gcgccacgcc
      121 gatggccacc agcgccgcca cgtcgcgatg gagcggcttg gggcccggct tggcgatgcc
      181 gagggtccgc cacgagctcc acgccccgtg gaagaggtga aggcccagcg ccaccatcgc
      241 cagcacgtag aaagcggtca cccaccagac cttgaagttc gcggtcaggt tggcgtagac
      301 gtcgccgggg acgaaggcgc cgcccgggtg gatgtcgccg gtggtgatgt tgaggatgtg
      361 gaacgggatg aacacgagga gcagcacccc accccatcgc agcgtgcgcg ccgcccaggt
      421 ggacacctgc ggctcccgtt tccggtactc gatcggccgc gcttcggcgt tgcggacggt
      481 gagccggtag gccatcagga cgtgcaccac cacggccacg agcagcacga agcgcgccag
      541 ccacagcacc tcgcccagcg ggccgtgcag gaggtggctg tagccgttga gcttgtcgcg
      601 gcccccgaag gccgggaaga cctgcaggtt ccccgccatg tggacgatca cgaagcccac
      661 gccgatgagc ccggagaggg ccatcagcac tttctgcccg atggtggagc gccagaggcg
      721 ggtgaacggg ttcatcgagt ggtcgagctc cctgggtgcg ctatccggta tccgctatcc
      781 gttatccgct atccgtgatc agatgttccg cgaatgcagc gacgccgtgc gggccgtcgg
      841 agcatcgggg agcggatgac ggatagccgt cctcacacga tcgccatcac gtcggtcacg
      901 gccttggccg aggcgtggag cgcagcccgc tcctcggcgg tcagggcgac ttcgagcacc
      961 gactccagcc cccgggcgcc gagcttgcac ggcacgccgc agaagacgtc cttgagcccg
     1021 tactcgccgg tgagccaggc ggagcacggc aggatccgct tcttgtcgag cgcaatggcc
     1081 tcgaccatct gcaccgccgc ggcgctcggc gcataatagg ccgagccggt cttcaagaac
     1141 gcgacgatct cggccccgcc gttgcgggcg cgggtcacga tggcgtcgag cttgtccttg
     1201 ccgaggagct gggtgatcgg gatgccggag accgtcgtat acgagatgag cgggaccatc
     1261 gtgtcgccgt ggccgccgag gaccatcgcc tggatgtcct cgacgctcac gtcgagcgcc
     1321 tcggccagga acgaccggta ccgcgccgtg tcgaggacgc cggccatccc gatcacgcgc
     1381 tcgcgcggga agccggtctc ccgcatcatg acgtagcaca tcacgtcgag cggattcgac
     1441 accacgatca cgatgctctt cggggcgacc ttcttgatgt tctccgccac cgacttgacg
     1501 atgccggcgt tggtcttgac caggtcgtcg cggctcatcc ccggcttgcg ggcgatcccg
     1561 gcggtcacga cgaacacgtc gctcccggcg gccgcctcat agctcgtggt gccgacgacc
     1621 cgggaatcga acccttcgat gggtgccgac tcccactggt cgagcccctt cccctgcggg
     1681 acgccgtcga ccacgtcgag caggacgacg gtgcgggcca ggctcttctc ggcgaggcgc
     1741 tgggccgcgg tggccccgac gttgcctgcg ccgacgacgg tgactttctg cagcataggg
     1801 cgttctggag ttcgggtcct tggcgtggtg tagcgcccgg tcccgcgatg ggccgagcaa
     1861 cccaatgtac cgactccccc ggcgagattg caggggcggc ggatctgcaa caccgacccg
     1921 tcgactcccg tactggtacc gaccttcttc caggacgacc gtgacccgat ccctctgcgt
     1981 cctcgcctcc gtgctcgcgc tggccacgcc gctcgcggcc caggacaact acgagatcca
     2041 ggtgtacggc tcggagaccg tcgcgccggc agtcacgatg ctggagctcc actccaactt
     2101 caccttcgac ggcggcacga ccgtcgtcga cggcgtgcgc cccaccgacc acgccctgca
     2161 cgagacggtc gaggtgaccc acggattcaa cgactggttc gaggtgggat tctacttctt
     2221 cacgacggcg caggacggag ggtggcacta cgtcgggagc catatccggc cccgggtccg
     2281 ggtgccgcca ggctggggct ggccggtcgg ggcgagcatt tcgcaggaga tcggctgcca
     2341 ggaccgctcc tatgcgacgg agcactgcaa ctacgagttc cggccgatcg tggacaagca
     2401 gatggggcgc ttctactggg cggtgaaccc gagcctcgaa ctggcgctca ccggccccga
     2461 ccgatcgatc gggttcgcgc cgagcgccaa ggtgagcttc gacttctcgc ggatcgtgac
     2521 ggggggattc gagtactacg gtggctgggg cccggtggcg gggctcctcc cgggcgatgc
     2581 gcgcgaggag gggctctacc ccaccatcga cctcaacgtt tcgccggagt gggaggtcaa
     2641 cgctggcgtc gggatcgggc tcacgccagc gaccgatcgg ctcctcgcca agctcatcct
     2701 gggacggcgc ctcggccgct aggccgttcg gctcctcccc cgtccgccgc ggcgggtccg
     2761 cccgcgcccg ggcacggcgg ccggcgcttc ggcccccgcg gctcccgcgt ccgcgtccat
     2821 cccgccttcc ggcacttcgt tcccccgccc cacctgccgc aatgcttcgt acagcacgat
     2881 ccccgcggcg gtcgccaggt tgagcgaccg gacgtcgccc ggcatcggga tcttgaaaca
     2941 ctgctccggg tgcttctcga tgatccgcgc cggcagcccc gtgtcctccc gcccgaacac
     3001 gaggacgctg ttcttctgat acggggcctc gagatagctc cgcgtggcgt gcgccgagaa
     3061 gtagatgcac cgctcgcggg agatggcctt tcggaactcg aaccagtccg ggtggcgcca
     3121 gaggtcgacc ttgtcccagt aatcgagtcc ggcgcgcttc aggtgcttgt cgtcgagcga
     3181 gaagccgagc ggctcgacca ggtggagcgc cgtgtcggtg gcagcgcaga gccgcgcgat
     3241 gttgccggtg ttgggcggga tctggggttc gagcagtgcg atgtggagca tgctgagacc
     3301 ggtaagcggt gagtggtgag cagtgagcgg tggacggtga acggtcaaca gggtacgggc
     3361 ctgtgccctg gcacctggag ctcggagcga aacctagcgc agcctacccg ccgcgggtgt
     3421 gcatctcccg atgcggatcg gtccgcagtc ccgcgagcgg caccaggacc ccgcggtcgg
     3481 gcgcgtggag ccgatcctcc gctccgcggt cgtcacgggc gctccaccgc ccggcgatga
     3541 tcgccgtcag ctccccgtcg ctcgtgccgt cgcggagcgg cccccggagg tcgaggccct
     3601 cccgtgcata gaggcaggtc aggaacgtcc cgtccgcggt cagccggccc cggtcgcagt
     3661 cggcgcagaa gggcgccgtc gtgctcgcca cgatgccgaa tacggtcccg tcggtcgacc
     3721 ggtatcgttc cgccggtgcg ctcccccgct ccgggagcgg ctccgccccg cccacgcgcg
     3781 agatgatctc gtcgcgggga acgacgtcgt ccatccgcca gcgcgtggcc ccgcccacgt
     3841 ccatgtattc gatgaaccgc acctcggcgc cgacctcgtt cccgaaggcg atgaggtcgg
     3901 cgagctcgtc atcgttgaac ccgcggatca ccacgacgtt caacttgatg ccgaatccgg
     3961 cgtcggccgc cgcccggatg ccgtcgagga tgtcggcatg gcgcgtggtg cgggagaact
     4021 ccttggcccg ctccgggcgc agcgtgtcga gactgacggt gacccgctcg agcccggcgg
     4081 ccttgaggcc ggcgacttcg tcccgcaaca gcagcccatt ggtcgtgagc gccaggtcct
     4141 tcggtcggac ctctgcgcgc aggagccgga cgagcgtcgg gaggtcgcgc cgcatcagcg
     4201 gctccccccc ggtcaggcgg atccgctcga cgccgagcga ggcgaagacg gtcgcgaggc
     4261 gcgtgatctc ctcgaaggac aggatcgact cccgcgggag ccagacgtag tcctcctccg
     4321 gcatgcagta cctgcaccgg aggttgcacc ggtcggtcac cgagatccgg agcgaacgga
     4381 tcggtcggcc ccggcggtcg gatgggcggg caggccgatg gccggacgcc tgggagccga
     4441 gcggcagcgg gtcaggcatc tggggccttc gcgggcaccg ttccccccgc cgcggtgggg
     4501 tcgacccatg ccaccgagcc gtcggcgtag tactccttct tccagatcgg gaccctccgc
     4561 ttcacctcgt cgatcacgaa gcgacaggcc tcgaaggcca cgtcccggtg gtcggcggcc
     4621 acgacgatgg ccacggcggc gtccccgatg gccagctggc ccacccggtg ccgcagggcg
     4681 acggccacgg gccagtggcg ccgcgcctct gccacgaccg ccgcacactc ggactcggcc
     4741 atttccgggt acgccgagta gtccaggcgg cggacagccc gtccggcgtg gtggtcacgc
     4801 acgatgccga cgaacgtgac gatcgcccca cgatccgggg ccgctacctc ggccgccagg
     4861 tcgtccacca cgaggcgatg ctccgtgagg aatggcacct cagcctccgg ccagtggcgg
     4921 cagcagggcg agctcgtcgc catccagcaa cggctcgttc ccccggacct gacggaagtt
     4981 acgggcgcac aagggcttgg gcggcaaagc gcgggcggag gggacgtcgg cgccgagctg
     5041 ggcaaggatg gaatcgacgg tggccggaac tgtcgccgtg accgagacca tgtcgcgccc
     5101 tacgacctcc gcataacgcg ccaggaaccg cacggtcact gtgatcgact tggcttgggg
     5161 cacctgcact cccggaaaac gcgaagggcc gcccgcaagc ggccccagga cccgacctcg
     5221 acccggctca ggtggccggg agctcactgg gcgcgtcctc ttccgcgacc agggcccgca
     5281 actccttgct gggcttgaac accggcaccg gacgggcagc cacttccacc gggtcgccgg
     5341 tgcgcggatt gcgcgccatg cgggtcttgc gccggcggat cttgaaggtg ccgaaccccc
     5401 ggact
//
LOCUS       DASUAP010000293         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_63754, whole genome shotgun sequence.
ACCESSION   DASUAP010000293 DASUAP010000000
VERSION     DASUAP010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63754"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1194
                     /locus_tag="VFU45_07850"
     CDS             79..1194
                     /locus_tag="VFU45_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEU5359017.1"
                     /translation="MSAKLQRWIDLLAALQRRRYPATFEELAKDVPGYQTRRGNAESL
                     RRMFERDKDELLAFGIPITSPALGDGDGGYAISAPAFYLPYLSLIAAGRPAKPTRTDR
                     WGYRSLAELAFEPDELRAIAAAATAAQRLGIPRLSADARSALRKLGHDLPPDALDAGA
                     AATQLLRRSSREEAALFDTLSAALQARKEVRFTYHSIGRNAVSPRTVHPCGLFFLGHW
                     YLAATTPDDATVKNYRLSRMTDVAVNGRRPGTPDYALPPSFVLADHARSRQSWELGGA
                     DAIEVTVRVVRESGAARAVTTLGAPAGSDPLLRRFTVRRPDAFCRWLLGFAGDVVPTG
                     PPSFVEQYAEEVRKVAALYAGADTAGEPRPRRGGGAA"
     gene            1191..2186
                     /locus_tag="VFU45_07855"
     CDS             1191..2186
                     /locus_tag="VFU45_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEU5359018.1"
                     /translation="MSKAKRGGKGTSDALATGAAEQLKRILALVPLCADDRPHRIAEV
                     AARAGTDEATLKRDLLALSQRFDDPGAFVEGVEIFLEADEFRVRSDHFLRPMRLTLAE
                     LAALDLGLALLRSESAVEERPVIERAGERLQAALARLPGAGPDDPLHHAAYAGNQVDE
                     GHLAELRRAIRTRRKARISYRKPDAAAAQSREVHPLGVVHASGHWYLVANGDEDEVLR
                     VFRLDRIEEVEVLTVAAQVPPGFSVDQLFRDGKAFVGEPAAELVVRYGPRIARWIAER
                     EGVPLEGDGSVVVRHPLADVDWAVRYVLQYGADAEVLAPDEVRVRMRERLRGIGV"
BASE COUNT          318 a          922 c          906 g          325 t
ORIGIN      
        1 ctccctcggg atgactccga tccccaccga cttccgcccc acccctcacc cccccgcgct
       61 acattctctc cacctcccat gtccgccaaa ctccagcgct ggatcgacct gctggcggcc
      121 ctccagcgcc gccgctaccc ggcgaccttc gaggagttgg ccaaggacgt ccccggttat
      181 cagacccggc ggggcaacgc cgagtcgctg cgccggatgt tcgagcgcga caaggacgag
      241 ctcctcgcct tcggcatccc gatcacctcg cctgcgctcg gcgacggcga cggcggctac
      301 gccatctccg cgccggcgtt ctacctcccc tacctctccc tcatcgccgc cggccgtccc
      361 gccaagccga cgcgcacgga ccgctggggg taccggagcc tggccgagct cgccttcgaa
      421 cccgatgagc tgcgggccat cgccgctgcg gccaccgcgg cgcagcgcct gggcattccc
      481 cgcctctccg ccgacgcccg ctccgccctc cgcaagctgg ggcacgacct tccccccgac
      541 gccctcgatg ccggcgccgc cgcgacccag ctcctccgcc ggagctcgcg ggaggaggcc
      601 gcgctcttcg acacgctgag cgccgccctc caggcgcgga aggaagtgcg cttcacctac
      661 cactcgatcg gccgcaatgc ggtgagcccc cgcaccgtcc acccctgcgg cctcttcttc
      721 ctgggacact ggtacctggc cgccaccacg cccgacgacg ccacggtcaa gaactaccgg
      781 ctgagccgga tgaccgacgt cgccgtgaac ggcaggcgcc cgggcactcc cgactacgcg
      841 ctcccgccca gcttcgtcct cgccgaccac gcccgttccc gccagtcgtg ggagctgggc
      901 ggcgccgatg cgatcgaggt gacggtccgc gtggtccgcg agagcggcgc cgcccgcgcg
      961 gtcacgacgc tcggcgcccc ggccgggagc gacccgctcc tccgccgctt caccgtgcgc
     1021 cgccccgacg ccttctgccg ctggctcctg ggatttgcgg gagacgtggt gcccaccggg
     1081 ccgccgagct tcgtcgagca gtatgccgag gaggtgcgga aggtggcggc gctgtatgcg
     1141 ggggcggaca ccgcgggcga gccgcgaccg cgtcggggag ggggcgccgc atgagcaagg
     1201 ccaagcgggg cgggaagggg accagcgacg cgttggcgac gggggcggcg gagcagctca
     1261 agcggatcct ggcgctggtg ccgctctgcg ccgatgaccg gccgcatcgg atcgcggagg
     1321 tggcggcccg ggcggggacc gacgaggcca ccctgaagcg cgacctcctg gccctctcgc
     1381 agcgcttcga cgatccgggg gcgttcgtcg agggggtgga gatcttcctc gaggccgacg
     1441 agttccgggt ccgctccgat cacttcctgc gcccgatgcg cctcacgctg gcggagctcg
     1501 cggcgctcga cctggggctc gcgctcctgc gctccgagag cgcggtggag gagcggccgg
     1561 tcatcgagcg ggcgggggaa cgcctgcagg cggcgctggc ccggctcccg ggtgcggggc
     1621 ccgacgaccc cctgcatcac gccgcgtacg ccgggaacca ggtggacgag gggcacctgg
     1681 ccgagctgcg gcgcgccatc cggacccggc ggaaggcgcg gatcagctac cggaagcctg
     1741 acgccgccgc cgcgcagtcg cgggaggtcc acccgctcgg cgtggtgcac gcctcggggc
     1801 actggtacct cgtggccaac ggcgacgagg acgaggtgct ccgcgtcttc cggctcgacc
     1861 ggatcgagga ggtcgaggtg ctgacagtcg cggcgcaggt gccgccgggg ttctcggtgg
     1921 accagctgtt ccgcgacggg aaggcgttcg tgggggagcc ggcggcggag ctggtggtgc
     1981 ggtacgggcc gcggatcgcg cggtggatcg cggaacggga aggggtgccg ctcgaggggg
     2041 acgggagcgt cgtggtgcgc cacccgctgg cggacgtgga ctgggcggtg cgctacgtgc
     2101 tgcagtacgg cgccgatgcg gaggtgctgg cgccggatga ggtgcgggtg cggatgcggg
     2161 agcggctgcg gggaatcggg gtgtaggtgc aggggcgcgg ggacgcaggg gcgcaggggc
     2221 gcgtgggggt cgcctgcggc gacccaagcc tcaccctccg gcccccgctc cttcgctgac
     2281 gcgatgatgg gaggcacgcc gtgcatcgga gtgtcatccc gagcgaagcg agggatctca
     2341 aaccgatcga tgcgtccagg acgccgctgc tgccccgggg caggccgggc atcggcttga
     2401 gatcactcgg tcgctccgct ccctcgggat gactccgagc cccaccgacg tccgccccac
     2461 ccgtcacccc c
//
LOCUS       DASUAP010000294         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_404608, whole genome shotgun sequence.
ACCESSION   DASUAP010000294 DASUAP010000000
VERSION     DASUAP010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404608"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1795
                     /locus_tag="VFU45_07860"
     CDS             59..1795
                     /locus_tag="VFU45_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5359019.1"
                     /translation="MHRSLVLTALCLMPSALLAQRPQEPAQPVTIVPQPETKLPLKHT
                     PEKTGPAITAQDLMTRLYIYSDDSMQGRETGKIGNVKATNYIEGQVRQMGLVPAGDNG
                     TYFQTIPFVTRTVDATSTFTVGGSSFAFGKDWAVQGGRTESLQNVGVVWGGNLADTSA
                     GDLTAQQVAGKILVLGYPQGPAGRRAMRRRPAAADSAAAIIIVGAGQFIPFLVRPTSF
                     VDDTTNPRNRASAPTLVLTDAAGARLFAQPLDSLKVGAAGNPFSLDLKVTVAPVAFPA
                     RNVVAILPGSDPVLKNEYVALGGHNDHIGMTNRPVDHDSVRIFNHIVRPNGADQGGKQ
                     ATPEEQTEVNAQLAAWRTAHPNSERLDSISNGADDDGSGTVSVLEIAQKMASLKHKPK
                     RSILFVWHVGEEKGLLGSAYFTDHPTVPRDSIVAQLNMDMVGRGDAWDVTGFTKDGAA
                     IHGSPNYIQLVGSRRLSTELGDLAEKVNKDDHHDLTFDYSIDADGHPANIYCRSDHYE
                     YARYGIPIIFFTTGLHSDYHQVTDEPEYIDYAHMARIDNYIEDLALHVADLDHRPVVD
                     HPKPDPHGACRQ"
     gene            1950..2348
                     /locus_tag="VFU45_07865"
     CDS             1950..2348
                     /locus_tag="VFU45_07865"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEU5359020.1"
                     /translation="MSRFVAFRRSLALSGLLLVPCVVAARPAEAFHLHLKRAEPGIND
                     TVAVAPKVIRLWFTERPEAAVARIQLQGPGGGWVAVAKPYLEPGDDAPLAAAVTGAMA
                     PGAYRVLWRAMAADGHPMRGSFGFVLKPAS"
     gene            2345..2959
                     /locus_tag="VFU45_07870"
     CDS             2345..2959
                     /locus_tag="VFU45_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU5359021.1"
                     /translation="MIRRSLLGVLVAGALLGCARGPEGSEFHGVVLPVPFPRPQVTLT
                     ATDGRPFDFAAATRGRVTFLFFGYTHCPDVCPVHMANLAAVLKDLTHEDRDRVQVVFV
                     TTDPARDSAQVLRRWLDQFDPTFIGLRGDDSTVARFEAEAHVAAAVKEAPTDSNAGYT
                     VGHAAQVIAFASDDSAHVLYPFGTRQEDWAHDIPLLLGGAWSGK"
     gene            2956..3426
                     /locus_tag="VFU45_07875"
     CDS             2956..3426
                     /locus_tag="VFU45_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEU5359022.1"
                     /translation="MTRRALAALGVAALVGCRPAPPVYHLGELTISHVVSPEPQRGGP
                     GGAYFTIVNGGPDDTLIGVASPDADTVMMHESETTGGMVKMDMTAAVPVPAGDTVRFA
                     PGRRHVMLTGLHRTLIAGDSLRLTLTFREAGTVVVTARVVAYADLDAALGAGGR"
     gene            3430..>3938
                     /locus_tag="VFU45_07880"
     CDS             3430..>3938
                     /locus_tag="VFU45_07880"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU5359023.1"
                     /translation="MLDIQWWCSARGLTWTWAWQAFPGVWIFIVALAVGLERCVRAAP
                     RPLAAWRTLLRAVVLVLLWATLDWPVGPLGAGYLMWVHAAQFLMLAMIAPPLLLLGLG
                     PEGAAPILRNRVMGPVIRAGTRPILAMIIFTAVMVISHLPGVVDALMKRQLGALALDL
                     AWLGSGILF"
BASE COUNT          558 a         1500 c         1281 g          599 t
ORIGIN      
        1 gaatagcgaa cgcctgttac ctgatcgcat cttttctctt ccccaacccc acgagaccat
       61 gcatcgatcc ctcgttctta ccgcgttgtg cctgatgccg agtgcgctgt tggcgcagcg
      121 gccgcaggag ccggcccagc cggtcaccat cgtgccgcag cccgagacca agctccccct
      181 caagcacacg ccggagaaga ccggtcccgc catcacggcc caggacctga tgacccggct
      241 ctacatttat tccgacgatt cgatgcaggg ccgggaaacg ggaaagatcg gcaacgtcaa
      301 ggcgaccaac tacatcgagg gccaggtgcg ccagatgggg ctggtccccg ccggcgacaa
      361 cggcacctat ttccagacca tccccttcgt gacccggacc gtcgacgcca cgtcgacctt
      421 caccgtcggt ggctcgagct tcgcgttcgg gaaggactgg gcggtgcagg ggggacgcac
      481 cgagtcgctc cagaacgtgg gcgtcgtgtg gggcggcaac ctggccgaca cctcggcggg
      541 tgacctcacc gcccagcagg tggcagggaa gatcctcgtc ctcggctatc cgcagggacc
      601 cgccggccgc cgggcgatgc gccggcgccc tgccgcggcc gactccgccg ccgcgatcat
      661 catcgtcggc gccgggcagt ttatcccctt cctggtccgt cccaccagtt tcgtggacga
      721 cactaccaac ccccgcaatc gcgcgtcggc gccgaccctg gtgctgaccg acgccgccgg
      781 ggcccggctc tttgcccagc cgctcgactc cctcaaggtc ggtgcggccg gcaacccgtt
      841 ctcgctcgac ctcaaggtca ccgtcgcccc ggtggcgttc ccggcgcgga acgtggtggc
      901 catcctcccc ggcagcgacc ccgtgctcaa gaacgagtac gtggcactcg gcggccacaa
      961 cgaccacatc ggaatgacca accgcccggt cgatcacgat tcggtgcgga tcttcaacca
     1021 catcgtccgg cccaacggcg ccgaccaggg cggcaagcag gcgacgccgg aggagcagac
     1081 cgaggtgaac gcccaactcg ccgcgtggcg gacggcgcac cccaactccg agcggctgga
     1141 ttcgatctcc aacggcgccg acgacgatgg ttccggcacg gtgagcgtgc tcgagatcgc
     1201 gcagaagatg gcctcgctca agcacaagcc gaagcgctcg atcctcttcg tctggcacgt
     1261 cggcgaggag aagggcctcc tcggctcggc gtacttcacc gaccacccga cggtgccgcg
     1321 cgattccatc gtggcccagc tcaacatgga catggtgggc cggggtgacg cgtgggacgt
     1381 gaccggcttc accaaggacg gcgcggccat ccacgggagc ccgaactaca tccagctggt
     1441 cgggtcccgc cggctgtcga cggagctggg cgacctggcg gagaaggtga acaaggacga
     1501 ccaccatgac ctcaccttcg actactcgat cgacgccgac gggcacccgg ccaacatcta
     1561 ctgccggagc gaccactacg agtacgcccg ctacggcatc ccgatcatct tcttcaccac
     1621 ggggctgcac tccgactatc accaggtgac cgacgagccg gagtacatcg actacgccca
     1681 catggcgcgg atcgacaact acatcgagga cctggccctg cacgtggccg acctcgacca
     1741 ccggccggtc gtcgaccacc cgaagcccga cccgcacggc gcctgccggc aatgacacgg
     1801 tagggggcgg cccgcggcag ccctggccgt tacccgcgcc atgggggtcg cctgcggcga
     1861 cccgatcccg gtggaccacg accccgtccg gcttcgcgcc gggcggggtc ttggtatctt
     1921 tccccgcacg ttctcccaag gatccgctcg tgagtcggtt cgtcgcgttc cgccggtcgc
     1981 tcgccctctc cggcctcctg ctcgtgccat gcgtcgtcgc cgcgcgcccc gccgaggcgt
     2041 tccatctgca tctcaagcgc gccgaacccg gcatcaatga caccgtggca gtggcgccga
     2101 aggtcatccg gctctggttc accgagcgcc cggaggccgc ggtggcgcgg atccagctcc
     2161 agggcccggg cggcgggtgg gtcgcagtcg cgaagccgta cctcgagccg ggagacgacg
     2221 cgcccctcgc cgccgccgtc accggcgcga tggcgcccgg cgcctaccgc gtcctctggc
     2281 gcgcgatggc ggccgacggc cacccgatgc ggggcagctt cggcttcgtc ctcaagccgg
     2341 cctcgtgatc cgtcgctccc tcctcggggt cctggtcgcc ggcgcgctcc tcggctgcgc
     2401 ccgcggcccc gaggggtcgg agttccacgg ggtcgtcctc ccggtgccgt tcccccgtcc
     2461 gcaggtgacc ctcaccgcca ccgatggccg ccccttcgac ttcgccgcgg ccaccagggg
     2521 gcgcgtcacc ttcctcttct tcggctacac ccactgtccc gacgtctgcc cggtgcacat
     2581 ggccaacctc gcggcggtgc tcaaggacct gacccacgag gaccgcgacc gggtgcaggt
     2641 cgtgttcgtc acgaccgacc cggcacggga ttccgcgcag gtcctccgtc gctggctcga
     2701 ccagttcgac cccacgttca tcggcctccg gggcgacgac tcgaccgtcg cgcgcttcga
     2761 ggcggaggcc cacgtcgccg ctgcggtcaa ggaggccccg accgactcga acgcgggcta
     2821 caccgtcggg cacgccgccc aggtgatcgc cttcgcatcc gacgacagcg cccacgtgct
     2881 ctatcccttc ggcacccgcc aggaggactg ggcccacgac atcccgctcc tcctcggcgg
     2941 cgcatggagc ggcaagtgac ccgccgcgcc ctggcggccc tcggcgtggc cgcgctggtc
     3001 gggtgccggc cggcgccgcc ggtgtaccac ctgggggagc tcacgatctc ccacgtcgtg
     3061 tccccggaac cgcagcgggg tggcccgggc ggcgcctact tcacgatcgt caatggcggc
     3121 cccgatgaca cgctcatcgg cgtcgcctct cccgacgccg acacggtgat gatgcacgag
     3181 tcggagacga ccggcgggat ggtgaagatg gacatgaccg ccgccgtccc cgtccccgcc
     3241 ggcgacaccg tgcgcttcgc gccgggccgg cgccatgtga tgctgacggg gctccatcgg
     3301 acgctgatcg ccggcgattc cctgcggctg accctcacct tccgcgaggc gggcaccgtc
     3361 gtggtcacgg cgcgggtggt ggcctacgcc gacctcgatg ccgccctcgg cgccggcggg
     3421 cgctgaccga tgctcgacat ccagtggtgg tgctccgccc gcggcctcac ctggacctgg
     3481 gcgtggcagg cgttccctgg cgtctggatc ttcatcgtgg cgctcgccgt gggactggaa
     3541 cggtgcgtgc gcgccgcgcc gcgaccgctg gcggcgtggc gcacgctcct ccgcgcggtc
     3601 gtgctcgtgc tgctctgggc caccctcgac tggccggtcg gccccctggg ggccggctac
     3661 ctgatgtggg tccacgccgc ccagttcctg atgctggcga tgatcgcgcc cccgctcctc
     3721 ctcctgggcc tcggaccgga gggcgcagcg ccgatcctcc gcaaccgggt catgggcccg
     3781 gtcatccgtg cgggcacccg gccgatcctg gcgatgatca tcttcacggc ggtgatggtg
     3841 atctcgcacc tgcccggcgt ggtcgacgcc ctgatgaagc ggcagctggg cgccctcgcg
     3901 ctcgacctgg cgtggctcgg ttccgggatc ctcttctg
//
LOCUS       DASUAP010000295         4641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1182070, whole genome shotgun sequence.
ACCESSION   DASUAP010000295 DASUAP010000000
VERSION     DASUAP010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182070"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFU45_07885"
     CDS             <1..402
                     /locus_tag="VFU45_07885"
                     /inference="COORDINATES: protein motif:HMM:NF020672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEU5359024.1"
                     /translation="VVRRPDFRAAVPGGEAELAALVGRVVAGGLTPPDVPELERITGR
                     QDVAAALRIAARDGRVVAVRRDWFVGAEALAGFADVLRGLGAGAPITVGDVRDRTGLS
                     RKYLIPLLEWADASGATERRGDVRVVLQRRA"
     gene            466..921
                     /locus_tag="VFU45_07890"
     CDS             466..921
                     /locus_tag="VFU45_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359025.1"
                     /translation="MRKRTIAAVLFGVATAAGPPVLAQTRPPPPTAELRENSPNPFFP
                     ATLIPFSINPSECANGHQPLVSLKVYNVLVQVVAIPVLQSSKGEVLNDIHIPCGDHVA
                     LWDGRMMDGPDEPTPGIYYSQLTVNGVRYTRKMIVRRPPGRRPKDASGT"
     gene            1105..3732
                     /gene="clpB"
                     /locus_tag="VFU45_07895"
     CDS             1105..3732
                     /gene="clpB"
                     /locus_tag="VFU45_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEU5359026.1"
                     /translation="MVRPDRLTLKAQEAFRDALADARARGNPVANDAHLLAALLAQPE
                     GVVRPLLQKAGVNVTDLTAQVAREIDRFPTQQGEAGEPALSREVNGAFDRAEREAKAL
                     GDAFVSTEHLLLGLMEEKGTAARALLSAAGLSSEDLRQALGEVRGAHRVTDQAPEEKY
                     QALERFTRNLTEQARAGKLDPVIGRDEEVRRVMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVQGDVPESLRGREIVALDIGQLLAGAKFRGEFEERLKAVLKELTQAEGRYV
                     VFIDELHTLVGAGKAEGAVDAGNMLKPALARGELHVVGATTLDEYRQHVEKDPALERR
                     FQPVLVGEPSVPDTIAILRGLKERYEVHHGVRITDDALVAAATLSDRYIGDRFLPDKA
                     IDLVDEAASRLRIEIDSLPQEIDEVERRIVQLEIERQALLKEKDRTARERRDALEREL
                     AQLREESGGMKAQWQAEKVAIQGMRGKKEELEALRTQVDQATRAGDLQKAAELRYGRI
                     PALERELAEGETRLGEIQKGAKYLKEEVDQEDIAEIVAKWTGIPVSKMLESDRERLTR
                     LEEELGRRVIGQGAAVSAVANAVRRSRAGLQDPNRPTGSFIFLGPTGVGKTETARALA
                     EFLFDDERAMVRLDMSEYMEKHAVARMIGAPPGYVGYEEGGQLTEAIRRRPYSVVLFD
                     EIEKAHPDVFNILLQILDDGRLTDSQGRVVDFRNTVIIMTSNLGGHLIVEAGAEAGAE
                     AWGMVERSVREELRQHFRPEFLNRVDEVIVFHPLDRDEIGRIVELQLEKLSRLLAGRQ
                     LSLTVTPEARALIGGEGYDPVYGARPLKRVIQQRLQNPLALAVLEGTYQPGDTVRVGV
                     TGKELSFARQAGRPEAASA"
     gene            3725..4195
                     /gene="tadA"
                     /locus_tag="VFU45_07900"
     CDS             3725..4195
                     /gene="tadA"
                     /locus_tag="VFU45_07900"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEU5359027.1"
                     /translation="MRRSAPAGGDLAGMEAALRLARAAAVRDEVPVGAVIIHEGETLA
                     EAHNETIQRNDPTAHAELLAIHHAMRHGYGARLLEATLYVTLEPCAQCAGAIVLARIR
                     RVVFGAFDQRAGMAGSVHDLLRHPRLNHRPEVLPGVLADECGELLKRFFRAKRE"
     gene            4350..>4641
                     /locus_tag="VFU45_07905"
     CDS             4350..>4641
                     /locus_tag="VFU45_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU5359028.1"
                     /translation="MLGPQARPWPDGEPLVEYLGQNGVSRREFLVFCGQMSAILGLTS
                     AATPQVARALSKQRRPSVVWLQLQECTGCVESVLRTEAPSIGDLLLNVISLDF"
BASE COUNT          714 a         1567 c         1710 g          650 t
ORIGIN      
        1 gtcgtccgcc ggcccgactt ccgggccgcg gtgcccggcg gggaggccga gctggccgcg
       61 ctggtcgggc gggtcgtggc gggtggcctc acgccgccgg atgtgcccga gctcgagcgg
      121 atcaccgggc ggcaggatgt cgccgccgcg ctcaggatcg cggcgcggga cgggagggtc
      181 gtcgccgtgc ggcgcgactg gttcgtcggc gcggaggccc tggcgggctt cgccgacgtc
      241 ctccgtgggc tcggggccgg ggcgccgatc accgtcggcg acgtgcgaga ccgcaccggc
      301 ctctcgcgga agtacctgat cccgctcctg gagtgggccg atgccagcgg tgccaccgag
      361 cgccgcggcg atgtgcgcgt ggtgctccag cgtcgagctt gacacgccat cggggcgcgg
      421 gaaggtttcg gtcagggtac ctgacgaggt ccattcggag atctgatgcg caagcgcacc
      481 atcgcggcag tgctgttcgg cgtggccacg gcggccggcc cgccggtgct ggcccagacg
      541 cggccgccgc ccccgacggc ggagttgcgg gagaattcgc ccaacccgtt cttccccgcg
      601 accctgatcc cgttctccat caatccatcg gagtgtgcca acggacatca acctcttgtc
      661 agtctcaagg tgtacaacgt gctggtgcag gtcgtcgcga tcccggtgct ccagagctcg
      721 aaaggtgagg tcctcaacga catccatatc ccgtgtggcg accacgtcgc cctgtgggac
      781 ggccggatga tggacggacc ggatgagccg acgccgggga tctactacag tcagttgacg
      841 gtcaatgggg tgcgttacac ccggaagatg atcgtccgac gccctccggg gcgccggccg
      901 aaggatgcga gcggaacgtg aaggcggccc ggtggccgcc ttcacgcgct tcggggactg
      961 tcaatccggc agggatctcc ggtacgccga ttgccttgtc agcggcccga tgtccgtgaa
     1021 cgctgcccgc cgtgcgttgc gcgcttcgag ccgatgcgcc gccggcgcct tcggtgtatt
     1081 actcattgat gtgaggagga tgtgatggtc cgtcccgatc gtctgacgct gaaggcgcag
     1141 gaggcgttcc gcgacgcgct ggccgatgcg cgtgcgcggg gcaacccggt cgcgaacgac
     1201 gcccacctgc ttgccgcgct gctggcgcag ccggaaggcg tcgtgcgccc gctcctgcag
     1261 aaggccggcg tcaacgtgac cgatctcacc gcccaggtgg cgcgggagat cgatcgcttc
     1321 cccacgcagc agggcgaggc cggggagccg gcgctgagcc gcgaggtgaa cggtgcgttc
     1381 gaccgcgccg agcgggaggc caaggcgctg ggcgatgcgt tcgtgtcgac cgagcacctc
     1441 ctgctcgggc tgatggagga gaagggcacg gcggcccggg cgctgctgag cgcggccggc
     1501 ctgtccagcg aggatcttcg acaggccctc ggggaggtcc gcggggccca tcgcgtcacc
     1561 gaccaggcgc cggaggagaa gtaccaggcg ctggagcgct tcacccgcaa cctcaccgag
     1621 caggcccggg ccggaaagct cgaccctgtc atcggccggg atgaggaggt gcgccgcgtc
     1681 atgcaggtgc tctcccggcg cacgaagaac aatccggtgc tgatcgggga gccgggggtg
     1741 ggcaagacgg ccatcgtcga ggggctggcc cagcggatcg tgcagggcga cgtccccgag
     1801 tcgctgcggg gccgggagat cgtggcgctc gacatcgggc agctgctggc gggtgccaag
     1861 ttccgcggcg agttcgagga gcggctcaag gcggtcctca aggagctgac ccaggcggaa
     1921 ggccgctatg tcgtgttcat cgacgagctg catacgctgg tcggcgccgg gaaggcggaa
     1981 ggggcggtgg acgccggcaa catgctcaag ccggcgctgg cccggggcga gctgcacgtg
     2041 gtgggcgcca ccaccctcga cgagtaccgt cagcacgtcg agaaggaccc cgcgctcgaa
     2101 cgccggttcc agccggtgct ggtgggcgaa cccagcgtgc ccgacaccat cgccatcctc
     2161 cggggcctca aggagcgcta tgaggtccac cacggcgtcc gcatcaccga cgatgcgctg
     2221 gtggcggcgg ccacgctgtc ggaccgctac atcggcgacc ggttcctgcc cgacaaggcg
     2281 atcgacctgg tcgacgaggc ggcgagccgc ctccggatcg agatcgactc gctgccgcag
     2341 gagatcgacg aggtcgagcg ccgcatcgtg cagctggaga tcgagcgcca ggcgctgctc
     2401 aaggagaagg accgcacggc ccgggagcgg cgggacgcgc tcgaacgtga gctggcgcag
     2461 ctgcgcgagg aaagcggcgg gatgaaggcg cagtggcagg ccgaaaaggt cgccatccag
     2521 gggatgcgcg ggaagaagga ggagctcgag gcgctgcgga cccaggtcga ccaggcgacg
     2581 cgcgcgggcg acctgcagaa ggcggcggag ctccgctacg gccggatccc ggcgctcgaa
     2641 cgggagctcg ccgaggggga gacgcgcctg ggggagatcc agaagggcgc gaaatacctc
     2701 aaggaggagg tcgaccagga ggacatcgcg gagatcgtgg ccaagtggac cggcatcccc
     2761 gtgtcgaaga tgctggagtc cgaccgggaa cggctcaccc ggctcgagga ggagctcggc
     2821 cggcgggtga tcgggcaggg ggcggcggtg tcggcggtgg ccaacgccgt ccggcggagc
     2881 cgcgccggac tgcaggaccc caaccggccc accggctcgt tcatcttcct cggccccacc
     2941 ggcgtgggga agaccgagac ggcccgcgcg ctggccgagt tcctgttcga cgatgagcgg
     3001 gcgatggtcc ggctcgacat gtccgagtac atggagaagc acgccgtggc ccggatgatc
     3061 ggcgcgcccc ccggctacgt gggctatgag gaaggaggcc agctcaccga ggccatccgg
     3121 cgccgaccgt acagcgtggt gctcttcgac gagatcgaaa aggcgcaccc cgacgtcttc
     3181 aatatcctgc tccagatcct ggacgatggc cgcctgaccg acagccaggg gcgggtggtg
     3241 gatttccgga acacggtcat catcatgacc agcaacctcg gcggccacct gatcgtcgag
     3301 gcgggagcgg aggcgggggc ggaggcgtgg gggatggtcg agcgctcggt gcgcgaggag
     3361 ctgcgccagc acttccggcc cgagttcctg aaccgggtgg atgaggtcat cgtcttccat
     3421 cccctcgacc gggacgagat cggcaggatc gtcgagctgc agctcgagaa gctctcgcgc
     3481 ctgctcgcag ggcgccagct ctcgctcacc gtcactccgg aggcccgggc gctgatcggc
     3541 ggcgagggct acgacccggt ctacggcgcc cggcccctca agcgcgtcat ccagcagcgg
     3601 ctgcagaacc cgctcgcact cgccgtgctc gagggcacgt accagcccgg ggatacggtg
     3661 cgggtcggcg tgacggggaa ggagctctcc tttgcccgac aggcggggcg cccggaggcg
     3721 gccagtgcgt aggtccgcac ccgcgggcgg cgacctggcc gggatggagg cggcgctccg
     3781 gctggcgcgc gccgcggccg tgcgtgacga ggtgccggtc ggcgccgtca tcatccacga
     3841 gggcgagacc ctcgcggaag cgcataacga gaccatccag cggaacgacc cgaccgcgca
     3901 cgccgagctc ctggccatcc accacgcgat gcggcatggg tacggcgccc gccttctcga
     3961 ggcgaccctc tacgtgaccc tcgagccctg tgcgcagtgt gccggcgcca tcgtcctggc
     4021 ccgcatccgg cgggtggtct tcggcgcgtt cgaccagcgg gcggggatgg caggctcggt
     4081 gcacgacctg cttcgccacc cccgcctcaa tcaccggccg gaggtcctcc cgggcgtcct
     4141 cgccgacgaa tgcggcgagc tgctcaagcg gttcttccgg gccaaacggg agtgaccgcc
     4201 atttcctgag cgttgcttca ccgatcgttc atctccccct cagtcggcct cccatacaat
     4261 cgtgctcgat cggccgcggt cccccgccga tggacgagca ccctgagccc gcccgaagcc
     4321 acccctcagc ggccgagtgg agcgtgcgca tgctcggccc ccaagcccgt ccctggcccg
     4381 acggcgaacc cctcgtcgag tacctcgggc agaacggcgt gtcccgccgc gagttcctgg
     4441 tcttctgcgg acagatgtcg gccatcctcg gcctcaccag cgcggccacg ccgcaggtgg
     4501 cccgtgcgct ttcgaaacag cgacgcccca gcgtcgtgtg gctccagctg caggagtgca
     4561 ccggctgcgt ggaaagcgtg ctgcgcacgg aggcgccgag catcggcgac ctgctgctca
     4621 acgtcatttc cctcgacttc t
//
LOCUS       DASUAP010000296         5026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_936661, whole genome shotgun sequence.
ACCESSION   DASUAP010000296 DASUAP010000000
VERSION     DASUAP010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936661"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFU45_07910"
     CDS             complement(<1..741)
                     /locus_tag="VFU45_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5359029.1"
                     /translation="MQLTHLILDVRDGIAWLTVDRPDKLNALNDALIEDLGHAARHVA
                     TDSAIRGAILTGAGSKAFVAGADIGELAAQSPQEGKARSQRGQAVFRAIEQCGKPVIA
                     AVNGFALGGGCELAMACHLRVASATAKFGQPEVKLGIGPGYGGTVRLPRLVGKGRALE
                     LLLTGRMIDADEAYRIGLVNKVVPPEGLAAAAEELLRTILDQGPLAVRLCIEAVDAGL
                     EMSLDEALLLEANHFGLLSGSADMKEGTA"
     gene            930..1829
                     /locus_tag="VFU45_07915"
     CDS             930..1829
                     /locus_tag="VFU45_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5359030.1"
                     /translation="MPNPRRNDVAMLAVVLIWAANFTALKVTLVEVPVLAIGAIRFVL
                     GAVALALVVRRREGDVRFPPGLLWRLTWLGVVGNTIYQTLFMVALDRTSVANAAILLA
                     TSPLLVAGLGAFTGVERLTRWVGAGLVLAFAGVVLVVGAHEASFTAATRVGDLAMLGA
                     SLCWAVFTLAVRRLPAEISALKVTALTTITGAPGLLLLGLPDLLHLRWGAVSVAAWGG
                     LAYSLFLSLVLAYVLWNASVKVVGPNRTAIYNCLVPLAAMLIAWRVLGEPIEAVQVVG
                     AALIVGGVLLTRNGHAPPVMEVA"
     gene            1826..3313
                     /gene="glpK"
                     /locus_tag="VFU45_07920"
     CDS             1826..3313
                     /gene="glpK"
                     /locus_tag="VFU45_07920"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU5359031.1"
                     /translation="MSAILALDQGTTGSTALVIHQDGAVLGRGYQEIHQHYPEPGWVE
                     HDPDELFTSLVAAGVQALAAARLSAREVAGIGIDVQRETVVLWDRATLRPVHNAIVWQ
                     DRRTAERCRELRAAGRAEWLSERTGLVPDPYFSATKIEWLLRDPDLRRRADRGELAVG
                     TVESWIVARLTSGRAHVTDHTNASRTLLYGLARRGWDPELLDLFGVPPELLPTIVRSS
                     GVVAECDTAPFGAALPVAGLVGDQQAALFGQGCVTPGSAKVTFGTGAFLLEDTGQELR
                     RSRHGLLTTAACGPAGEPAYAVEGSLFIAGAAVQWLRDGLGVIERASETEAIARSVPD
                     AGGVHFVPAFAGLGSPYWDPEARGTITGITGGTRRAHLVRATLEAMAFGCADLLHAMA
                     DDTGVPLATLRVDGGAAANDWLMQFQAGIVGATVERPDVVDTTALGADALAGLALGVW
                     RSIEEFQAGRTYARFLPDRSAAERELARAGWRRAVEASLAWARMR"
     gene            complement(3315..4736)
                     /locus_tag="VFU45_07925"
     CDS             complement(3315..4736)
                     /locus_tag="VFU45_07925"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU5359032.1"
                     /translation="MRRPVLLAALLALATGVPLSAQQQADTGRFDHARHAKLFPSCIS
                     CHGGADDPALPIYPAATVCATCHDGALRSKVTWTPPSAARASNLRFAHDVHRAALARA
                     GKDSATCITCHAPSGARWMTAVRFPVPESCVGCHAPGGAHLTQADSACTQCHETLAAS
                     PLPATEVAKFPAPPSHRDSAFTLGGGHGRAAKASNTSCALCHAQDFCATCHANAAQVP
                     AIRSLAADPRSLALKAPAAVPPSHSDPRFDRKHGALARRSGATCATCHSQESCTSCHQ
                     GAVPEQIRRLSAALPGRPSGAILVRRPPPSHADPSWARSHGADAAARPQSCSACHARS
                     QCLECHRPDAGAATGYHPAGFLTRHPAEAYARESSCAECHNTRGFCQDCHRTAGVTAT
                     GPLKPGFHDAQGAFLLSHGQAARQGLETCVSCHAERDCLTCHSAAGGRGFDPHGPGFD
                     AAQLRRKNPTMCVACHGTNIPSK"
     gene            complement(4733..>5026)
                     /locus_tag="VFU45_07930"
     CDS             complement(4733..>5026)
                     /locus_tag="VFU45_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359033.1"
                     /translation="LTWRAGSRLSLSAHGGTLDRPLEYRYDVASLAWYGASADLRVNE
                     RLRLGANLDRWNEDRSRPDAAGISWDQTRFSVSASWILVSDADRLPLPPARAQ"
BASE COUNT          644 a         1815 c         1821 g          746 t
ORIGIN      
        1 cgccgtccct tccttcatat cggccgaacc cgagagcagc ccgaagtggt tggcctccag
       61 gaggagcgcc tcatccaggc tcatttcgag tccagcgtcc accgcctcga tgcagagccg
      121 gaccgccagc ggaccctggt cgaggatggt ccgcagcagc tcctcggccg cggccgccag
      181 cccctccgga ggcaccactt tgttcaccag tccgatccgg tacgcctcat cggcgtcgat
      241 catccggccg gtgagcagga gttccagggc gcgtcccttg ccgaccaggc gggggagccg
      301 caccgtgccc ccgtagccgg gaccgatgcc gagcttgacc tccggctggc cgaacttggc
      361 cgtggcgctc gccacccgga ggtggcaggc catcgcaagc tcgcagcccc cgccgagtgc
      421 aaagccattg accgcggcga tgacgggctt gccgcattgc tcgatcgcac ggaacaccgc
      481 ctgtccccgc tgcgaccgcg ccttcccctc ctgcggcgac tgcgcggcga gctcgccgat
      541 gtcggcaccg gccacgaagg ccttgctgcc ggcgccggtc aggatcgcac cgcggatagc
      601 ggagtcggtg gccacgtgcc gggccgcgtg acccaggtct tcgatgagcg cgtcgttcag
      661 ggcgttcagc ttgtcgggcc ggtcgacggt gagccaggcg attccgtcac ggacgtcgag
      721 gatcaggtgg gtgagctgca tgggtttggc tcgcggcgag aggtccaggg tggtgcgcca
      781 tccgttatcc gcaatccgat gatcgacggg gatcccacgg ccctctggat ccggcggagt
      841 cggttctcca ccccgacggt cggatcgcgg ctggcggaca gcgagtgcag ccgacgaaat
      901 ctccgaaaag atacactaca ttccgctcca tgccgaaccc tcgccgcaac gacgttgcga
      961 tgctcgccgt ggtgctcatc tgggcggcga acttcacggc cctcaaggtg acgctcgtcg
     1021 aggtgccggt gctcgccatc ggcgccatcc gcttcgtgct cggcgccgtt gcgctggcgc
     1081 tcgtcgtccg gcgccgcgag ggcgacgtgc gatttccgcc cggactcctt tggcgcctca
     1141 cctggctcgg cgtggtcggc aacacgatct accagaccct gttcatggtg gccctcgacc
     1201 gcacgtccgt ggccaatgcc gccatcctcc ttgccacgtc gcccctcctg gtggcgggcc
     1261 tcggcgcctt caccggcgtc gaacggctga cgcgctgggt cggtgcgggc ctggtgctgg
     1321 cgttcgccgg tgtggtgctc gtcgtcggcg cgcacgaggc ctcgttcacc gccgccaccc
     1381 gcgtcggcga ccttgcgatg ctcggcgcct cgctctgctg ggcggtgttc accctcgcgg
     1441 tccgccggct gccggcggag atctcggccc tcaaggtcac cgcgctcacc accatcaccg
     1501 gtgcccccgg tctcctcctc ctcggcctcc ccgacctcct ccaccttcgc tggggggccg
     1561 tctccgtggc cgcctggggg gggctcgcct attccttatt cttatccctc gtgctggcgt
     1621 atgtgctctg gaacgccagc gtcaaggtcg tgggtcccaa ccgcacggcg atctataact
     1681 gcctcgtgcc gctcgccgcg atgctgatcg cctggcgcgt cctgggggag ccgatcgagg
     1741 cggtgcaggt ggtcggcgcc gcgctgatcg tcgggggcgt gctcctgacc cggaacggcc
     1801 acgccccgcc ggtcatggag gtcgcgtgag cgccatcctc gcgctcgacc agggcaccac
     1861 cgggagtacc gcgctcgtga tccatcagga cggcgcggtg ctgggccggg ggtaccagga
     1921 gatccatcag cactacccgg aaccggggtg ggtggaacac gatcccgacg agctgttcac
     1981 gagcctcgtc gccgccggcg tgcaggcgct tgctgcggcg cgcctctccg ccagggaggt
     2041 ggccgggatc ggcatcgacg tccagcggga gacggtcgtg ctctgggacc gggccaccct
     2101 ccggccggtc cacaacgcga tcgtgtggca ggaccggcgc accgccgagc ggtgccggga
     2161 gctccgggca gcggggaggg ccgagtggct gtcggagcgc accgggctcg tgcccgatcc
     2221 ctacttctcc gcgaccaaga tcgagtggct gctccgcgat cccgacctcc ggcgccgcgc
     2281 tgaccgcggc gagctggccg tcgggacggt cgagagctgg atcgtggccc ggctcacgtc
     2341 ggggcgggcc cacgtcaccg accacaccaa tgccagccgc acgctgctct atggccttgc
     2401 gcgccgcggg tgggacccgg agctgctgga cctcttcggc gtgccgcccg agctcctgcc
     2461 gaccatcgtc cgatcgagcg gcgtcgttgc cgaatgcgat acggccccct tcggcgccgc
     2521 gctccccgtc gccggcctcg tcggcgacca gcaggcggcg ctctttggcc agggctgtgt
     2581 cacccccggg tcggccaagg tcaccttcgg cacgggcgcc ttcctcctgg aagacaccgg
     2641 acaggagctc cggcgctccc ggcacggcct gctcacgacc gcggcgtgcg gccccgccgg
     2701 cgagccggcc tatgcggtcg aggggagcct cttcatcgcc ggcgccgccg tgcagtggct
     2761 ccgcgacggg ctcggcgtca tcgaacgcgc ctcggagacc gaggccatcg cccgcagcgt
     2821 gccggatgca gggggcgtgc atttcgtccc cgcgttcgcg ggcctcggct ccccgtattg
     2881 ggatccggag gcgcgcggga cgatcaccgg gatcaccggc ggcacccgtc gcgcccacct
     2941 ggtgcgcgcc accctggaag cgatggcgtt cgggtgcgcg gacctgctgc acgcgatggc
     3001 cgatgacacc ggggtcccgc tcgccacact ccgcgtcgat ggcggcgcgg cggccaacga
     3061 ctggctgatg cagttccagg cgggcatcgt cggcgccacg gtcgagcgcc ccgacgtggt
     3121 cgacacgacc gccctcggcg ccgacgccct ggcagggttg gcgctcgggg tgtggcgctc
     3181 catcgaggag ttccaggccg gccgcacgta cgcccgcttc ctgcccgacc gctccgccgc
     3241 cgagcgtgaa ctggcccgcg ccggctggcg acgcgccgtg gaggcgagcc tcgcctgggc
     3301 ccggatgcgc tgacctactt cgacgggatg ttggtcccgt ggcaggccac gcacatcgtc
     3361 gggttcttcc gtcggagctg cgcggcgtca aagcccggcc cgtgcgggtc gaagccgcgc
     3421 ccgcccgccg ccgagtggca cgtcaggcaa tcccgctcgg cgtgacagct cacgcacgtc
     3481 tccaacccct ggcgcgccgc ctgcccgtgc gacagcagga acgccccctg cgcatcgtgg
     3541 aagccgggct tgagcggtcc cgtcgccgtc acgcccgccg tccgatggca gtcctggcag
     3601 aacccccgcg tgttgtggca ctcggcacag gaactttcgc gggcatacgc ctcggccgga
     3661 tggcgggtca ggaacccggc ggggtggtat ccggtcgcgg cgcctgcatc gggccggtgg
     3721 cattccaggc actgggagcg cgcgtggcac gccgagcagg actgcgggcg tgcggcggcg
     3781 tcggccccgt gggaccgcgc ccacgacggg tcggcgtgcg agggcggggg ccggcgcacg
     3841 aggatcgcgc ccgagggacg ccccggcagc gccgcactga gccgacggat ctgttcgggc
     3901 acggcgccct ggtggcagct cgtgcagctc tcctgcgaat ggcaggtggc gcaggtggca
     3961 ccggagcgac gcgccagcgc gccgtgctta cggtcgaacc gcgggtccga gtgcgagggt
     4021 ggcaccgcgg ccggcgcctt gagcgccagc gatcgcgggt cggcggccag cgaccggatg
     4081 gccgggacct gcgcggcgtt ggcgtggcag gtggcgcaga agtcctgggc atggcacagg
     4141 gcgcaggacg tgttcgacgc cttcgcggcg cgcccgtgcc cgccgcccag cgtgaacgcg
     4201 ctgtcccgat gggaaggcgg ggcggggaac ttcgccactt cggtcgccgg caacggtgag
     4261 gccgcgagcg tctcatggca ctgcgtgcag gcgctgtcgg cctgggtcag gtgcgccccg
     4321 cccggagcgt ggcagccgac gcagctctcc ggcaccggga atcgcactgc cgtcatccac
     4381 cgcgcgccgc tcggcgcgtg acaggtgatg cacgtggccg agtccttgcc tgcgcgggcg
     4441 agcgcggccc gatggacgtc gtgggcgaac cggaggttgg atgcccgcgc cgccgatggg
     4501 ggcgtccagg tgaccttcga ccgaagcgca ccgtcgtgac aggtggcgca gaccgtggcc
     4561 gccggataga tcggcagcgc gggatcgtcg gccccgccgt ggcaggagat gcacgacggg
     4621 aacagcttgg cgtgcctggc gtggtcgaac cgcccggtgt cggcctgctg ctgggcggag
     4681 agcgggacgc cggtcgccag cgccagcaga gcggcaagga gaacgggccg cctcactggg
     4741 ccctcgccgg tggcagtggc aggcggtcgg catccgaaac gaggatccag ctcgccgaga
     4801 ccgagaatcg ggtctggtcc cacgagatcc cggcggcgtc cgggcggctg cggtcctcgt
     4861 tccatcggtc gagattggcg ccgagccgca gacgctcatt cacgcgcagg tcggccgagg
     4921 cgccgtacca cgcaagtgac gcgacgtcgt agcggtactc gagtggtcgg tcgagcgtgc
     4981 caccgtgggc cgagagcgaa agccgggaac cggcccgcca tgtgag
//
LOCUS       DASUAP010000297         5015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_36291, whole genome shotgun sequence.
ACCESSION   DASUAP010000297 DASUAP010000000
VERSION     DASUAP010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36291"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2105
                     /locus_tag="VFU45_07935"
     CDS             <1..2105
                     /locus_tag="VFU45_07935"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEU5359034.1"
                     /translation="RVNLLAAVAGAATVAFLALAFLEILRRREAGSGSGSGSGAGAEA
                     GAVAAALVAGFTLTHWQNSNETEVYSIATAMVGAVLWLALRWRGERDAAAAPGATPRP
                     DRSSRYLLLILYIFGLAVGDHLLTLLAGPAVVAFLWAEERRVTVEVAVVAAVWAVLVA
                     TGLGSTMLLLVAGAGLVTAFVVAARARRLDVAVLGAAVAALGVSTYLFLLVRSGQHPF
                     LNEAQPDTWERMVAVIRRAQYPVRTPFDDPTIAHGATNPGRSLGMIWLQLQDYAQYFS
                     WQWGAALPDVARGVVATAFASLGFFGSRAQWREDRSAWWLCFVLWVVSGLGLVAYMNF
                     KPGASHAWAQYPDPADHEVRDRDYFFVLSFLVWGCWAGMQMARLASEWWGRRGGWRIA
                     GGVAAACILTPLALNWPTATRLGPDRTLARDAAWDLLNSVGPYGILVTYGDNDTFPLW
                     YLQEVEGVRRDVTVVCLALANTDWYVRQLRDVPVRPFDEAAAPPVWRGLHPVAPDRPL
                     LAMSDSEISDIAGRLIPVTRTDTLTLGPIRQVIPAGVTLYPNQFVVLRILQQNLGRRP
                     FAWSVTAGREFLGLEDRVVLQGMAWRVEPGAIDSSSARFGGGRVAGVLVDVPLTDSLL
                     WGTFRYGSLVATGVGGLEPAAQGLASDLGLPFTYLASIAQRSGDRVAAVRNLKVAVQL
                     APSEQLKLILSNLESGGR"
     gene            2158..2841
                     /locus_tag="VFU45_07940"
     CDS             2158..2841
                     /locus_tag="VFU45_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5359035.1"
                     /translation="MEYPGLPGHLERVRATIAEAQAAGGWSHPVTIVAVTKTHGPEAV
                     RAAYAAGLRDVGENRVQEALPKQDACADVPVAWHLIGSLQRNKARQVVGRFRLIHSVD
                     RLELGQELARRAAPGRQGILVQVNCSDEPQKGGVAPGELPRLLDDLLALDGLEVRGLM
                     TMAELTADAGAQRAAFRRLRDLRDAAERRGIPLPELSMGMSGDYPVAVAEGATMIRLG
                     TILFGGRTP"
     gene            2838..3353
                     /locus_tag="VFU45_07945"
     CDS             2838..3353
                     /locus_tag="VFU45_07945"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEU5359036.1"
                     /translation="MTDDAFELTPHDVRSQEFSRGFGGYDRAQVDDFRLRLADALDRL
                     LRERAQVEERLRGMQEQLRVFRERERAMNEALVSAQQLRADTKVQADREAESLLREAK
                     AEALRIVQQAQEQEKVIRERYEAASRMFASYLANFRALLERQMGEVEGLQASAQTRAQ
                     VQTELILKRHA"
     gene            3388..>5015
                     /locus_tag="VFU45_07950"
     CDS             3388..>5015
                     /locus_tag="VFU45_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5359037.1"
                     /translation="MSYPAWPAGPADQLERELLDRWKAEGLFARVQAQGKDAPGFVFF
                     EGPPTANGKPGIHHVFARTIKDLICRYQSLLGRRVTRIAGWDTHGLPVEIEVEKLLKI
                     SGKKDIEAYGVEKFNALCRESVFRYRDEWEKLSDRIAYWLDYEHPYITCSAEYVESVW
                     AILKRLHERDLLYRGHRVLPYCPRCGTVLSSHELALGYEEIQDNSIYVSFPLTDGSGR
                     ELVVWTTTPWTLPSNVAVAVNPELTYGAYASKKHPGRAFIIAADRAGALANVLGGELA
                     PVGEAFPGQALVGLEYRRPLDVVPLPDDRKHQVVVPGDFVTAHDGSGLVHMAPAFGSD
                     DFAAGKEHGLALVRPVAADGTFQGTTWPELEGKLVTDKATNDLIIRKLKELGRWLHTE
                     PHQHSYPHCWRCQSKLIYYARDSWFVRTSAVKDRMLELNRSVGWHPPEVGSGRFGEWL
                     ENNVDWALSRDRYWGTPLPVWVCDRDPAHVDVVGSYAELAERWGKPLPADFDPHKPHV
                     DGYVWPCACGGLKHRAPEVIDTWFDSGSMPYAQW"
BASE COUNT          691 a         1764 c         1798 g          762 t
ORIGIN      
        1 ggcgggtcaa tctccttgcc gccgttgccg gagccgcgac ggtggcattc ctggcgctcg
       61 cgttcttgga gatactgcgt cgccgtgagg cgggttcggg ttcgggttcg ggttcgggtg
      121 cgggggcgga ggccggcgcc gtcgccgcgg cgctggtggc ggggttcacc ctcacccact
      181 ggcagaattc gaacgagacc gaggtctact cgatcgccac ggcgatggtg ggcgcggtgc
      241 tctggctggc attgcgctgg cgcggggagc gggacgccgc cgccgctccc ggcgcgacgc
      301 ctcgccccga tcggtcgtcg cgctacctgc tcctcatcct ttacatcttc gggctcgcgg
      361 tcggcgatca cctcctgacg ctgctggcgg ggccggcggt cgtcgcgttc ctctgggccg
      421 aggagcggcg ggtgacggtc gaggtggcgg tcgtggccgc ggtatgggcg gtgctcgtgg
      481 cgaccgggct cggctcgacg atgctcctgc tcgtggcagg tgcggggctc gtcacggcgt
      541 tcgttgtcgc ggcgcgcgcc cggcggctcg acgtggcggt gctcggcgcc gccgtcgccg
      601 cgctcggtgt ctcgacctat ctcttcctcc tggtccgttc cggccagcac cccttcctca
      661 acgaggccca gcccgacacg tgggagcgga tggtcgccgt gatccgacgg gcacagtacc
      721 ccgtgcggac gccgttcgac gacccgacca tcgcccacgg cgccaccaat ccggggcgaa
      781 gcctgggaat gatctggctc cagctgcagg actacgccca gtacttctcg tggcagtggg
      841 gcgcggcact cccggacgtc gcgcgcggcg tcgtcgcgac cgcgttcgcg tcgctcggat
      901 tcttcgggag ccgggcgcag tggcgcgagg atcgctcggc ctggtggctc tgcttcgtcc
      961 tctgggtcgt gtcggggctc ggcctcgtgg cctacatgaa cttcaagccc ggcgccagcc
     1021 acgcgtgggc acagtacccc gaccccgcgg atcacgaggt ccgggaccgc gactacttct
     1081 tcgtgctgag cttcctcgtc tggggatgct gggcgggaat gcagatggcg cgcctggcgt
     1141 cggagtggtg ggggcgtcgt ggcgggtggc gaatagcggg aggcgtggcc gccgcgtgca
     1201 tcctgacgcc actcgccctc aactggccca ccgccacccg gctcggcccc gaccggacgc
     1261 tcgcgcgcga cgccgcgtgg gatctcctca attcggtggg gccctacggc atcctcgtca
     1321 cctatggcga caacgacacc ttcccgctct ggtacctgca ggaggtggag ggggtccggc
     1381 gcgacgtgac cgtcgtctgc ctggcgctcg ccaacaccga ctggtacgtg cggcagctcc
     1441 gcgatgtgcc ggtgcggccg ttcgacgagg cggcggcccc gcctgtctgg cgcgggctcc
     1501 acccggtggc gcccgaccgt ccgctccttg cgatgagcga ctccgagatc tccgacatcg
     1561 ccgggcggct catcccggtg acgcgcaccg acacgctcac gctggggccc atccggcaag
     1621 tcatccccgc gggcgtcacc ctctatccca accagttcgt ggtgctccgg atcctgcagc
     1681 agaacctggg gcggcgcccc ttcgcctggt cggtcacggc ggggcgggag ttcctggggc
     1741 tcgaggaccg tgtggtgctg caggggatgg cgtggcgggt ggagccgggc gccatcgaca
     1801 gcagctccgc gcggttcggc ggcgggcggg tggcgggcgt gctggtggac gtgccgctca
     1861 ccgactcgct cctctggggc acgttccgat acggttcgct ggtcgccacc ggtgtcggcg
     1921 gccttgagcc ggcagcgcag gggctcgcgt ccgacctggg gctgccgttc acgtatctcg
     1981 cgtcgatcgc ccagcggagc ggtgatcgcg ttgccgccgt ccggaacctc aaggtcgctg
     2041 tgcaattggc gccgagcgag cagctcaagc tgatcctcag caacctggag tcgggcggca
     2101 ggtagcttcg tgggcgcgac tttcaccgtt ccgtaactcc aagtaaattc aacgactatg
     2161 gaatacccgg ggcttcccgg ccacctcgag cgcgtgcggg ccacgatcgc ggaggcgcag
     2221 gcggcggggg gatggagcca ccccgtcacc atcgtggcgg tgaccaagac ccacggaccc
     2281 gaggcggtgc gcgcggcgta cgcggccggg ctccgcgacg tgggggagaa ccgggtccaa
     2341 gaggcgctcc ccaagcagga cgcctgcgcc gacgtccccg tggcgtggca cctcatcggg
     2401 agcctccagc ggaacaaggc gcgccaagtc gtcggccggt tccggctcat ccattcggtg
     2461 gaccgcctgg agctcgggca ggagctggcg cggcgcgcgg cgcccggccg ccagggcatc
     2521 ctggtgcagg tcaactgctc ggatgagccg cagaagggcg gggtggcccc aggcgaactc
     2581 ccccggctcc tcgacgacct gctggcgctc gacggcctcg aggtccgggg cctcatgacg
     2641 atggcggagc tgaccgcgga cgcgggcgcc cagcgcgccg ccttccgccg gctccgcgac
     2701 ctgcgcgacg cggcggagcg ccgcggcatt ccgctccccg agctctcgat ggggatgtcg
     2761 ggcgactacc cggtggccgt cgctgaaggt gccacgatga tccggctcgg cacgatcctg
     2821 ttcggaggac gcacgccatg accgacgacg cattcgagct gacgccgcac gacgtgcggt
     2881 cgcaggagtt ctcccgcggt ttcggcggct acgaccgcgc ccaggtggac gatttccgtc
     2941 tccggctggc cgacgcgctc gaccggctcc tgcgcgagcg cgcacaggtg gaggagcgcc
     3001 tccgggggat gcaggagcag ctgcgggtct tccgcgagcg ggaacgcgcg atgaacgagg
     3061 cgctcgtctc cgcccagcag ctgcgggccg acaccaaggt gcaggccgac cgcgaggccg
     3121 agtcgctcct gcgcgaggcc aaggcggagg cgctccggat cgttcagcag gcgcaggaac
     3181 aggagaaggt catccgggag cggtatgagg cggcgagccg gatgttcgcc tcctacctgg
     3241 ccaacttccg cgccctcctc gaacgccaga tgggcgaggt cgaggggctg caggccagcg
     3301 cgcagacccg cgcccaggtg cagaccgagc tgatcctcaa gcgccacgca tgaccctgcc
     3361 gctccacgcc gcaccacggc gggcccgatg agctacccgg cctggcccgc cggtcccgcc
     3421 gaccaactcg agcgcgagct cctcgaccgc tggaaggcgg aggggctctt tgcgcgtgtc
     3481 caggcgcagg ggaaggacgc cccggggttc gtcttcttcg agggcccgcc caccgccaac
     3541 ggcaagcccg gcatccacca cgtattcgcg cgcaccatca aggacctgat ctgccgctat
     3601 cagtcgctcc tcggcaggcg ggtcacgcgt atcgccggct gggacaccca cggacttccg
     3661 gtcgagatcg aggtcgaaaa gctcctcaag atctcgggca agaaggacat cgaggcgtac
     3721 ggcgtcgaga agttcaacgc cctctgccgt gagagcgtct tccgctaccg cgatgagtgg
     3781 gagaagctct ccgaccggat cgcgtactgg ctggactacg aacaccccta catcacctgc
     3841 tcggcggagt acgtcgagtc ggtgtgggcc atcctcaagc gcctgcacga gcgcgacctg
     3901 ctctaccgcg gccaccgcgt gctgccctac tgcccccgct gcggcacggt gctctcgagc
     3961 cacgagctgg cgctcggcta cgaggagatc caggacaact ccatctacgt gagcttcccg
     4021 ctcaccgacg ggagcgggcg cgagctcgtg gtttggacca cgacgccgtg gaccctgccg
     4081 agcaacgtgg cggtggcggt caatccggag ctgacctacg gcgcgtacgc gagcaagaag
     4141 catccggggc gcgcgttcat catcgccgcg gaccgcgcgg gagcgttggc caacgtgctg
     4201 ggcggcgagc tcgcgcccgt gggcgaagcg ttccccggcc aggcgctcgt cggcctcgag
     4261 taccggcgcc cgctcgacgt cgtgcccctg cccgacgacc ggaagcacca ggtcgtcgtc
     4321 cccggggact tcgtcaccgc gcacgacggg tcggggctgg tccacatggc gccggccttc
     4381 gggtcggacg attttgccgc cggcaaggag cacgggctgg cgctcgtccg ccccgtggcg
     4441 gcggacggca ccttccaggg caccacctgg ccggagctcg aggggaagct cgtcaccgac
     4501 aaggcgacca acgacctcat catccggaag ctcaaggaac tcggccgctg gctccacacc
     4561 gagccccacc agcacagcta tccgcactgc tggcgctgcc agagcaagct gatctactac
     4621 gcccgcgact cctggttcgt ccgcacgtcg gcggtcaagg accggatgct ggagctcaac
     4681 cgctcggtgg ggtggcaccc gcccgaggtg gggagcgggc gcttcggcga gtggctcgag
     4741 aacaacgtcg actgggcgct ctcgcgcgac cgctactggg gcacgccgct ccccgtatgg
     4801 gtgtgcgacc gggatccggc ccacgtggac gtcgtcggga gctatgccga gctggccgag
     4861 cgctggggca agcccctgcc ggcggacttc gacccccaca agccgcacgt cgacgggtac
     4921 gtctggccct gcgcgtgcgg cggtctcaag caccgcgcgc cggaagtgat cgacacctgg
     4981 ttcgactcgg gctcgatgcc gtacgcccag tggca
//
LOCUS       DASUAP010000298         6780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1208833, whole genome shotgun sequence.
ACCESSION   DASUAP010000298 DASUAP010000000
VERSION     DASUAP010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208833"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFU45_07955"
     CDS             <1..317
                     /locus_tag="VFU45_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5359038.1"
                     /translation="NVFHAGDGNLHPTISFDGRDAALAERVHRASGEIVAACVAAGGS
                     ITGEHGVGVDKREYMPLLFSPDTLSAMCAVRRVFDPLDTANPVKVIPIHACREWRAGG
                     VA"
     gene            314..1528
                     /locus_tag="VFU45_07960"
     CDS             314..1528
                     /locus_tag="VFU45_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5359039.1"
                     /translation="MSTAILERLTGLLGPACVGRDPSGLPRAMPDSSDAAALVLRLAH
                     DEGWKVRLEGRGSWLPADAPADLALSSRGLDQVVSVSPADLVATVQAGVTFDTLERRL
                     AAAGMWLPLDPPGRPDRSVGSIIATATTGPLRAGAGTVRDHVLGCTFVTGDGKVVDAG
                     GRVVKNVAGYDLTKLQVGGFGGFGMLTELHLRLRARPEQDVTLVAAGDRDRLTRAGRS
                     AIEAGVTALALELCAPAVVAEREWQLAARLAGPAAGASAESVALAEATGLEWRTLAVE
                     QAHAWWTMAARAPLGGAVTLRLGALPDGLDETLDLLDEVLDAGLVTVGTTTGQVRWSG
                     DAHAASIKRVRAVAASREVPLTLERAPWTLRREVGHFGAYREGVGPIVGRLRDTFDPG
                     HNFVVALEGDDA"
     gene            1521..2759
                     /locus_tag="VFU45_07965"
     CDS             1521..2759
                     /locus_tag="VFU45_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEU5359040.1"
                     /translation="MRERPTALSVSPALDPCVHCGFCLPACPTYLATGDEADSPRGRI
                     VLMRALAQGELAPHDPAVTEHLEHCLGCRGCEPACPSGVEYGRGLEATRAWLAERNGI
                     PPLARIVLRVFRHRWLWVPLLTAARWARDLGLARAFSGKGRIGFGFASLAASRGLSST
                     SRDAPSSRTPTRPPVQLFTGCVMSTLFDHVNDATARVLAANGHTIVPARGQRCCGALH
                     AHAGDLDGARRLAEGNVRAFAGSDAIIVVNSAGCGALLKDYGHLLGTADAERFAARVR
                     DVTEVLADDGPRAGAPIEWKVAYDAPCHLQHAQHVHQQVLTMFAAIPGLDVTLAPGSD
                     RCCGSAGIYSLLEPGMSRAVLDAKVEELRTAAPDLVATGNPGCLMQVGAGLAVAGVPA
                     RAVHPVELLDASYQAAGAYR"
     gene            2770..3762
                     /locus_tag="VFU45_07970"
     CDS             2770..3762
                     /locus_tag="VFU45_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359041.1"
                     /translation="MAELTPLEVGPAVDAVQLAAVQDRAVLVPTAAAVIDLSGTGAVD
                     CLQGLLTNDVAKPGPDSVTYGALLTPKGMIVTDLWALRTAAGFTLVVPAAGHAAAAEI
                     LHRTLPPRLARSTDLSATHRAVTLFGAGSEAAITAALPGHEIPAPGRLTYVGPSAVAH
                     APAGAPFRWLIVGPLADVEALESALRTQGVQTGIEAQATAARILAGWPALGLEIDEKT
                     LPQEVRFDEIGGVSYTKGCYTGQETVARVHFRGHPNRELRAVVWDGSDPLADRSIQHL
                     GREVGTVRSTVVVGARRIGLAPIRREVQNDEVVSAGGSPARVVALPLPVATLFP"
     gene            3759..4745
                     /locus_tag="VFU45_07975"
     CDS             3759..4745
                     /locus_tag="VFU45_07975"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5359042.1"
                     /translation="MILLTGGTGLVGLAILDELKSLGLGCTALVRTAEGAAVVSARGA
                     IPLQGSVEDPAVWARVGGISAVIHAAALLRSHGGWAPFARVNVGGTGLAAARARGLGA
                     RFIHISSVAVYGDVSRRPDASVAEEHPWAPLPSGNLYARSKRDAEQVVWSEAARGLAA
                     IVLRPCPVYGPGDRLFVPRLWAAARRGWMPLVGPGDRPVPLVHARSVAQAVGAALRAS
                     DGWGRAYNVTGDGAVTARQIVAAAARGAGRGIRTIAVPERLAFGMADAADLARRVLPA
                     GRMPGTVRTGVGYWRGGDPFDSGAAAAALGWAPKVVHAEMIERLIAERAAAE"
     gene            complement(4807..5034)
                     /locus_tag="VFU45_07980"
     CDS             complement(4807..5034)
                     /locus_tag="VFU45_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359043.1"
                     /translation="MKKALFLVALVGMAACAEKKPAAPAADSAAMAAPAAAMPADTSK
                     KMADTGAMKKDTGMKMAAPAAAPAPAKPAKK"
     gene            5218..6660
                     /locus_tag="VFU45_07985"
     CDS             5218..6660
                     /locus_tag="VFU45_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU5359044.1"
                     /translation="MTDHLPQTLGELKSSAWASPPLRGRTVRDELRANLLRAIEAGEE
                     LFPGIVGFEDTIEPQIVNALLARHHFILLGLRGQAKSRILRQLARFLDERIPAIAGSE
                     VNDDPFHPISKFGREQVATLGDRTPIAWLERDQRYVEKLATPDVTIADIIGDVDPIRA
                     ARGGHVLSDELTMHFGLLPRANRGIFALNELPDLSGKIQVGLFNVLQEGDVQLKGYPV
                     RLPLDVLLVFTANPEDYTARGKIITPLKDRIGSEITTHYPRTVDLGQAITRQEAWLVR
                     GGLPVEIPPFVAEVVERVAFEAREDRRVDRRSGVSQRLPISVLENVVSNAERRAIAHH
                     GRAVEPRISDIYAALPSITGKLELEYEGELQGADVVARDLIRRAAGKAFDEALGDVDF
                     RPTVAWFDQGGALKVNVDERTELCLKGFSVIPGLVEAVEAAGLGHRKTAPGALVAGCE
                     LALEGLAAHQRITRSEELGYTRVRQARRET"
BASE COUNT          907 a         2484 c         2449 g          940 t
ORIGIN      
        1 gcaacgtctt ccacgccggc gacgggaacc tccaccccac catctccttc gacggacgcg
       61 acgccgcgct ggccgagcgg gtacaccggg ccagcgggga gatcgtggcc gcctgcgttg
      121 cggccggcgg gagcatcacc ggggagcacg gcgtcggcgt ggacaagcgg gaatacatgc
      181 cgctgctctt ctctcccgac accctctcgg ccatgtgcgc ggtgcgccgc gtcttcgacc
      241 cgctcgacac ggccaatccc gtcaaggtga tccccatcca cgcctgccgc gagtggcggg
      301 ccggaggcgt cgcgtgagca ccgccatcct cgaacgcctc accgggctcc tcggcccggc
      361 atgcgtcggc cgcgacccgt cgggcctgcc gcgcgcgatg cccgacagca gcgacgccgc
      421 cgcactcgtg ctgcggctgg cccacgacga gggatggaag gtccgcctcg aagggcgggg
      481 atcgtggctc cccgccgacg cacccgccga cctggcgctc agttcccggg gcctcgacca
      541 ggtcgtgagc gtgagccccg cggacctcgt ggccaccgtc caggcagggg tgaccttcga
      601 cacgctcgag cggcgccttg ccgccgcggg gatgtggctc ccactcgatc cccccgggcg
      661 tcccgaccgg agcgtgggct cgattatcgc caccgccacc accgggccac tccgcgccgg
      721 cgccggcacc gtccgggacc acgtcctcgg ctgcactttc gtgaccggcg acggcaaggt
      781 ggtcgacgcg ggcggtcggg tggtcaagaa tgtcgccggc tacgacctca ccaagctcca
      841 ggtcggcggc ttcggcggct tcggaatgct gaccgaactc cacctgcggc tccgcgcgcg
      901 cccggaacag gacgtgacgc tggtggccgc gggcgatcgc gaccggctga cccgcgcggg
      961 gcggtcggcg atcgaggcgg gcgtgaccgc gctggcgctc gagctgtgcg caccggccgt
     1021 ggtggcggaa cgggagtggc agctggcggc gcgccttgcc ggaccggcgg cgggcgcgag
     1081 cgcagagtcg gtcgcactcg ccgaggcgac cgggctcgag tggcggacgc tcgccgtcga
     1141 gcaggcgcac gcctggtgga cgatggcggc gcgggccccc ctcggcgggg ccgtgaccct
     1201 gcgcctcggc gcgctccccg acggcctcga cgagaccctc gacctcctcg atgaagtgct
     1261 ggatgcggga ctcgtcaccg tgggcaccac caccggccag gtgcgctgga gcggcgacgc
     1321 ccacgccgcg tccatcaagc gggtccgcgc cgtggccgcg tcgcgcgagg tcccgctcac
     1381 gctcgagcgg gccccctgga cgctccggcg cgaggtggga cacttcgggg cgtaccggga
     1441 gggcgtcggg ccgatcgtgg gacgcctgcg ggacaccttc gaccccggcc acaacttcgt
     1501 cgtggcgctg gaaggtgacg atgcgtgagc ggccgaccgc gctctcggtg agccccgcgc
     1561 tcgatccctg cgtccactgc ggattctgcc ttcccgcctg ccccacctac ctcgccaccg
     1621 gcgacgaggc agacagcccc cgcgggcgga tcgtgctcat gcgggcgctg gcccaggggg
     1681 agctcgcgcc ccacgacccg gcggtcaccg aacacctgga gcattgcctc ggctgtcgcg
     1741 ggtgcgagcc ggcgtgcccg tccggcgtcg aatatggccg gggcctcgag gccacccgcg
     1801 cgtggctcgc cgaacggaac gggatcccgc ccctcgcgcg catcgtgctg cgcgtgttcc
     1861 gccaccgatg gctctgggtg ccgctcctca cggcggcgcg ctgggcacgg gacctcggcc
     1921 tggcccgggc gttctccggc aagggccgga tcggattcgg cttcgcgtcg ctggcggcga
     1981 gcagggggct cagctcgacg tcccgcgacg ctccgtcctc ccgaacgccg acccggccac
     2041 cggtccagct gttcacgggc tgcgtgatgt cgaccctctt cgatcatgtg aatgacgcca
     2101 ctgcccgggt gctcgccgcg aacgggcaca ccatcgtgcc ggcccggggc cagcggtgct
     2161 gcggcgccct gcatgcccat gcgggcgacc tcgacggcgc acgtcgcctg gccgaaggga
     2221 acgtgcgggc cttcgcgggg tccgacgcga tcatcgtcgt caacagcgcc gggtgcggcg
     2281 ccctcctcaa ggactacggg cacctcctcg gtacggcgga tgcggagcgc ttcgccgcgc
     2341 gggtgcgcga tgtcaccgag gtgctggccg acgacggtcc gcgggccggc gcgccgatcg
     2401 aatggaaggt ggcctatgat gccccctgcc atctccagca cgcccagcac gtccaccagc
     2461 aggtcctcac gatgttcgcc gccatcccgg gcctcgacgt cacgctcgcc cccggatccg
     2521 atcgctgctg cggcagcgcg gggatctatt cgctcctgga gcccgggatg tcgcgcgccg
     2581 tgctcgacgc caaggtggag gaactccgga cggccgctcc cgacctcgtc gcgaccggca
     2641 atcccggatg cctgatgcag gtcggggcgg gactcgccgt ggccggcgtg ccggcgcggg
     2701 cggtccaccc ggtcgaattg ctcgatgcat cgtaccaggc ggcgggagcg taccgttaga
     2761 tttcaaccca tggcagaact caccccgctc gaggtcggac cggcggtcga cgcagtgcag
     2821 ctcgcggcgg tgcaggaccg cgcggtgctg gtgccgacgg ccgcggcggt gatcgacctg
     2881 agtggcaccg gcgccgtcga ctgcctgcag ggactcctca ccaatgacgt ggccaagccg
     2941 gggccggact ccgtcaccta tggcgcgctc ctcacgccga aggggatgat cgtcaccgac
     3001 ctctgggcgc ttcgcacggc cgccgggttc acgctggtcg ttcccgccgc cggacacgcg
     3061 gccgccgcgg agatcctcca tcggaccctc ccgccgagac tggcccgcag caccgacctt
     3121 tccgccaccc accgtgccgt gaccctcttc ggcgccgggt ccgaggctgc gatcacggcg
     3181 gcgctcccgg gccacgagat cccggcacca ggacggctta cctacgtcgg cccgagcgcc
     3241 gtggcgcacg ccccggcggg agcccccttc cggtggctca tcgtcgggcc gctggcggac
     3301 gtcgaggcgc tggagtcggc gctccgcacg caaggcgtcc agacggggat cgaagcccag
     3361 gccaccgccg cccggatcct ggccggctgg ccggcgctcg gcctcgagat cgacgagaag
     3421 acgctgccgc aggaggtccg cttcgacgag atcggcggcg tgagctacac caaggggtgc
     3481 tacaccggcc aggagaccgt ggcgcgcgtc cacttccgcg gccaccccaa ccgggagctg
     3541 cgggccgtcg tctgggacgg gtcggacccg ctggccgacc ggtccatcca gcacctcgga
     3601 cgcgaggtcg ggacggtccg ctccacggtc gtggtcggcg cccgccgcat cggcctcgcg
     3661 ccgatccgac gggaggtcca gaacgacgaa gtggtctcgg ccggcgggtc ccccgcgcgc
     3721 gtcgtcgctc ttcccctgcc ggtggccacg ctcttcccgt gatcctcctc acgggaggca
     3781 ccgggctcgt cggcctcgcc atcctcgatg aactgaagtc gctgggtctc ggctgcaccg
     3841 ccctggtgcg gacggccgaa ggcgcggcgg tggtcagcgc ccggggtgcg atccccctcc
     3901 agggaagcgt cgaggatccg gcggtatggg ctcgcgtcgg cggcatcagc gccgtgatcc
     3961 acgccgccgc actgctgcgt tcgcacggag ggtgggcccc cttcgcacgg gtcaacgtcg
     4021 gtggcaccgg gctcgcggcg gcgcgggccc gggggctggg tgcccggttc atccacatct
     4081 cttccgtcgc ggtctacggc gacgtttcgc ggcggcccga cgcctcggtg gccgaggaac
     4141 atccctgggc gccgctcccg tcgggcaacc tgtacgcgag gtccaagcgc gatgccgagc
     4201 aggtggtctg gagcgaagcg gcgcgcgggc ttgcagccat cgtgctccgt ccctgtccgg
     4261 tctacggccc cggcgaccgg ctcttcgtgc cgcgcctgtg ggccgctgcc cggcgcgggt
     4321 ggatgccgct cgtcgggccg ggggaccgcc cggtgccatt ggtccatgcg cggagcgtgg
     4381 cccaggccgt cggggcggcg ctcagggcat ccgacggatg ggggcgggcg tacaacgtga
     4441 ccggggacgg cgccgtcacg gcgcgccaga tcgtggccgc cgccgcccgt ggcgccggcc
     4501 gcggcattcg gaccattgcg gtgccggagc ggctggcctt cgggatggcg gacgccgccg
     4561 atctggcgcg gcgggtgctt ccggcgggcc ggatgccggg gacggtgcgg acgggggtcg
     4621 gctattggcg gggcggggat ccgttcgaca gcggggcagc ggcggcggca ttggggtggg
     4681 cgccgaaggt ggtgcacgcg gagatgatcg agcgcctgat cgcggagcgg gcggccgccg
     4741 aatgaacgaa ggccccggac cggggtccgg ggccttcgcg agacactgcg actggcgtca
     4801 ggaagcttac ttcttggccg gcttggccgg agcgggcgcg gcggccgggg cggccatctt
     4861 catcccggtg tccttcttca tggcaccggt gtccgccatc ttcttgctgg tgtcggccgg
     4921 catcgcggcg gccggagcgg ccatcgcggc ggagtcggcg gccggcgcgg ccggcttctt
     4981 ctcggcgcag gcggccatgc cgacgagcgc cacgaggaac agggccttct tcatgggatc
     5041 ctctagggtg tgtgagttgc cagtgggtgt ctcgggaacc gggccgtcac tgtgccggcg
     5101 tcagcgggca gaaaatacag gataccctta gactgtcaag ggtttcggcg ttcccggcct
     5161 ccgccgagca cgcacgccaa ggaccggccg ccgagtgcac cccaacggga aacgacgatg
     5221 accgaccacc tcccccagac cctgggtgaa ctcaagtcca gcgcgtgggc ctccccgccc
     5281 ctgcgtggcc gcaccgtgcg cgacgagctc cgcgccaacc tgctccgcgc catcgaggcc
     5341 ggcgaggagc tcttccccgg catcgtcggg ttcgaggaca ccatcgagcc ccagatcgtc
     5401 aacgcgctcc tggcgcggca ccatttcatc ctcctcggcc tccgcggcca ggccaagagc
     5461 cgcatcctgc gccaactggc tcgcttcctc gacgagcgca tccccgccat cgccgggagc
     5521 gaggtgaacg acgacccctt ccacccgatc tccaagttcg gccgcgagca ggtggccacc
     5581 ctcggcgacc ggacccccat cgcctggctc gagcgcgacc agcgctacgt cgaaaagctg
     5641 gccacccccg atgtcaccat cgccgacatc atcggcgacg tcgacccgat ccgcgccgcc
     5701 cgcggcggcc acgtgctctc cgacgagctc acgatgcatt tcggcctcct cccacgcgcc
     5761 aaccgcggga tcttcgcgct caatgaactg ccggacctga gcgggaagat ccaggtcggg
     5821 ctcttcaacg tgctgcagga gggcgacgtc cagctcaagg gctatccggt ccggctgccg
     5881 ctcgacgtcc tgctcgtctt caccgccaat cccgaggact acacggcgcg cggcaagatc
     5941 atcacccccc tcaaggatcg catcgggtcc gagatcacca cccactaccc gcgcacggtg
     6001 gacctcggcc aggccatcac ccggcaggag gcctggctcg tgcggggcgg gctccccgtc
     6061 gagatcccgc ccttcgtggc ggaggtggtg gaacgggtgg cgttcgaggc gcgggaggac
     6121 cggcgggtcg accggcgctc gggcgtgagc cagcggctcc cgatctcggt cctcgagaac
     6181 gtggtgtcca atgccgagcg ccgggccatc gcccatcacg ggcgcgcggt ggagccgcgg
     6241 atcagcgaca tctatgcggc gctcccgtca atcaccggca agctggagct ggagtacgag
     6301 ggggagctgc agggggccga cgtggtcgcc cgcgacctca tccgtcgcgc cgccggcaag
     6361 gcgttcgacg aggcgctcgg cgacgtggac ttccgcccga ccgtcgcctg gttcgaccag
     6421 gggggcgccc tcaaggtgaa cgtcgacgag cggaccgagc tctgcctcaa gggattctcg
     6481 gtcatccccg gcctggtcga ggcggtcgag gcggcgggcc tcggccaccg gaagactgct
     6541 ccgggcgcgc tggtggcggg gtgcgagctc gccctcgaag gcctggccgc gcaccagcgc
     6601 atcacccgga gcgaggaact gggctacaca cgggtccgac aggcgagacg ggagacgtga
     6661 gaagtgaaga gggaagaggg aaaagggaaa ggtgaaaagg gagaagggga cagcggactg
     6721 gttcgagccg gccgtcgtcc tgcctttcgc ccgatcgttc gctcggccca ccatgacgac
//
LOCUS       DASUAP010000299         3126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_813828, whole genome shotgun sequence.
ACCESSION   DASUAP010000299 DASUAP010000000
VERSION     DASUAP010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813828"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFU45_07990"
     CDS             <1..682
                     /locus_tag="VFU45_07990"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU5359045.1"
                     /translation="AYCAVSHGTPKAFDPLEPVRLAEAVAQMGLEHVVITSVDRDDLE
                     NGGAEAFAGCITEIKRRLPETSVEVLIPDFKGSERALQIVMDARPDILNHNLETAERL
                     YRLARPGGRYDRALKLLANARRMGGPGALTKSGIILGMGEEWDEVIRCLKDLRRSDVD
                     IVTLGQYLRPSEGHLPVVRYYTPDEFAELARIGRRLGFKHVQASPLTRSSYHAWEQVE
                     LARTPSET"
     gene            682..1701
                     /gene="pdhA"
                     /locus_tag="VFU45_07995"
     CDS             682..1701
                     /gene="pdhA"
                     /locus_tag="VFU45_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEU5359046.1"
                     /translation="MAAASSTALPKKIDAQTADLYRTYLRQMLQIRRFEEKAGEAYSL
                     GKIGGFCHLYIGQEAVAVGTLAALRHDDYLTCSYRDHGHALARGMSARAVMAELFGKA
                     AGCTKGKGGSMHLFDASLGFLGGHGIVGGHIPLTTGIAFAIKYRGGDQVALCYFGEAA
                     VNNGAFHEALNMAALWKLPAIYICENNRYGMGTALERASAIYDISERACSYDMANEVV
                     DGQDVLAVRAAMDRACARARKENLPTLLEIRTYRFMGHSMSDPIHGHYRTKEEVDEQR
                     KRDPITTWGDRLVAAGVIDEAGIKQIDAEVTAEAADAVQFADEAPEPAPEELWADVYA
                     PAGGH"
     gene            1704..2681
                     /locus_tag="VFU45_08000"
     CDS             1704..2681
                     /locus_tag="VFU45_08000"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEU5359047.1"
                     /translation="MAVLTYRDALNQALREEMQRDPSVFLMGEEVGVYNGAYKVSRGL
                     LEEFGPMRVVDTPITELGFAGVGVGAAMVGLRPIVEFMTWNFALLAVDQIVNSAAKMR
                     YMSGGQVGCPIVFRGPGGAALQLASQHSQAFESWYAHIPGLKVVMPATPADAKGLLKS
                     AIRDDDPVVFIEGEMLYNNKGEVPDGEHVVPIGQADVKRDGGDVTIICHSKTVAVALK
                     AAEQLATEGISAEVVDLRTIRPLDRETILTSVGKTHRVVVAEEGWGFAGVGAQVVDLI
                     QQEAFDELDAPVLRVTGADVPMPYNKFLERAAKADAPKVVAAVKKVMYV"
     gene            2776..>3126
                     /locus_tag="VFU45_08005"
     CDS             2776..>3126
                     /locus_tag="VFU45_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU5359048.1"
                     /translation="MATKVVMEALSPTMEEGRLVEWKKKEGDTVAVGDVLAEVETDKA
                     VMELVARAAGTLLKLAVPVGTTVPVANVVAWIGTPGESVGDGAQAPAVTPARASAPAS
                     APAPARAVAPVASVP"
BASE COUNT          529 a         1046 c         1122 g          429 t
ORIGIN      
        1 cgcgtactgc gccgtatccc acgggacgcc caaggcgttc gaccccctgg agccggtgcg
       61 cctcgccgag gcggttgcgc agatggggtt ggagcacgtc gtgatcacgt cggtcgaccg
      121 cgacgacctg gagaacggcg gcgccgaggc gttcgccggg tgcatcaccg agatcaagcg
      181 acgcctcccc gagacatcgg tcgaggtgct catcccggat ttcaaggggt cggagcgggc
      241 gctccagatc gtgatggacg cgaggcccga catcctgaac cacaacctcg agacggccga
      301 gcgcctctat cggctggcgc gccccggggg ccgatacgac cgggccctga agctgctggc
      361 caacgcccgg cggatgggcg ggcccggcgc gctcaccaaa tcggggatca tcctcgggat
      421 gggcgaagag tgggacgagg tgatccgctg cctcaaggac ctgcggcgga gcgatgtcga
      481 catcgtgacc ctcggccagt acctccggcc cagcgaggga cacctccccg tcgtgcggta
      541 ctacaccccg gacgaattcg cagagctcgc gcggatcggc cgacgactcg gcttcaagca
      601 cgtccaagca agcccgttga cccgctcctc gtaccacgcc tgggagcagg tggaactggc
      661 ccggacccct tcggagacct gatggccgca gcctcatcga ccgccctgcc caagaagatc
      721 gacgcgcaga cggcggacct ctaccgcacg tacctccggc agatgctcca gatccggcgc
      781 ttcgaggaga aggccggcga ggcgtacagc ctcgggaaga tcggcggctt ctgccacctc
      841 tatatcggcc aggaagcggt ggcggtcggc acgctcgcgg cgctccgcca cgatgactac
      901 ctgacctgca gctaccgcga ccacggccac gcgctggccc gggggatgag cgcgcgcgcg
      961 gtgatggccg agctcttcgg gaaggcggcc ggctgcacca aggggaaggg gggctcgatg
     1021 cacctcttcg acgcgtcgct cgggttcctg ggcggccacg ggatcgtcgg ggggcacatc
     1081 ccgctcacga ccggcatcgc cttcgcgatc aagtaccggg gcggcgacca ggtggcgctc
     1141 tgctacttcg gcgaggccgc ggtcaacaac ggggccttcc acgaggcgct caacatggcc
     1201 gcgctctgga agctcccggc catctacatc tgcgagaaca accgatacgg gatgggcacc
     1261 gcgctcgagc gcgccagcgc catctacgac atctccgaac gggcctgctc gtacgacatg
     1321 gccaacgagg tggtcgacgg gcaggacgtg ctggcggtgc gggcggcgat ggaccgcgcc
     1381 tgcgcccggg cccgcaagga gaacctcccc accctgctcg agatccgcac ctaccgcttc
     1441 atggggcact cgatgtccga cccgatccac gggcattatc gcaccaagga ggaggtcgac
     1501 gagcagcgca agcgcgaccc catcaccacc tggggcgacc ggctggtggc agccggggtc
     1561 atcgacgagg cggggatcaa gcagatcgac gcggaggtca ccgccgaggc ggcggacgcc
     1621 gtgcagttcg ccgacgaagc gccggagccg gcccccgagg agttgtgggc cgacgtctac
     1681 gcgccggcgg ggggacactg accatggccg ttctcaccta ccgcgacgcc ttgaaccagg
     1741 cgctccgcga ggagatgcag cgggacccgt cggtcttcct gatgggcgag gaagtgggcg
     1801 tctacaacgg cgcctacaag gtgagccgcg ggctcctgga ggaattcggg ccgatgcggg
     1861 tggtcgacac gccgatcacc gagctcggct tcgccggcgt cggcgtggga gccgcgatgg
     1921 tgggcctccg gccgatcgtg gaattcatga cctggaactt cgcgctgctg gccgtggacc
     1981 agatcgtcaa ctccgccgcc aagatgcgct acatgagcgg cggccaggtc ggctgcccga
     2041 tcgtcttccg cgggccgggc ggggcggcgc tgcagctcgc ctcccagcat tcgcaggcgt
     2101 tcgagagctg gtacgcgcac atccccggcc tcaaggtggt gatgcccgcg acgccggccg
     2161 atgccaaggg tctcctcaag agcgccatcc gcgatgacga cccggtggtg ttcatcgagg
     2221 gcgagatgct ctacaacaac aagggcgagg tccccgacgg cgaacacgtc gtccccatcg
     2281 gccaggccga cgtgaagcgc gacggcgggg acgtcaccat catctgccac tccaagaccg
     2341 tggcggtggc cctcaaggcg gccgagcagc tcgcgaccga ggggatctcg gccgaggtgg
     2401 tggacctcag gaccatccgc ccgctcgatc gggagaccat cctcacctcc gtcggcaaga
     2461 cccaccgggt cgtggtggca gaggaggggt gggggtttgc cggggtcggc gcgcaggtgg
     2521 tggacctgat ccagcaggag gcgttcgacg agctggatgc gccggtgctc cgggtcaccg
     2581 gcgccgacgt gccgatgccg tacaacaagt tcctcgagcg cgccgccaag gcggatgcgc
     2641 cgaaggtggt cgcggcggtc aagaaggtga tgtacgtctg aagtcactgg tcgttggtca
     2701 ttggtcgtcg acctccgaca cgatcggcat caacgacgaa cgaccaaggg ctaacgaccc
     2761 accccggata ccgacatggc aacgaaagtc gtgatggaag cgctctcccc gacgatggag
     2821 gaggggcgcc tggtcgagtg gaagaagaag gaaggggaca cggtcgccgt cggcgacgtc
     2881 ctggccgagg tcgagaccga caaggccgtg atggagctgg tggcgcgcgc ggccgggacg
     2941 ctcctcaagc tcgccgtgcc ggtgggcacg acggtgccgg tggcgaacgt ggtggcctgg
     3001 atcggcacgc cgggggagtc ggtgggtgat ggtgcccagg cgccggcggt aaccccggcg
     3061 cgggcgagcg cgcccgcatc tgcgcccgcg ccagcgcgcg cggtcgcacc ggtcgcttcc
     3121 gtgccg
//
LOCUS       DASUAP010000300         9177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_78523, whole genome shotgun sequence.
ACCESSION   DASUAP010000300 DASUAP010000000
VERSION     DASUAP010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78523"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFU45_08010"
     CDS             complement(<1..296)
                     /locus_tag="VFU45_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359049.1"
                     /translation="MIHRRVYAGVAVTALVLAQACSPNGSTGPTTGVNSSPELVTAHV
                     LTGQPYGVAIASTGQGYVTRLNLALLARVDLGTIVDSAATTGYAPTGVAFLPDGS"
     gene            complement(353..1273)
                     /locus_tag="VFU45_08015"
     CDS             complement(353..1273)
                     /locus_tag="VFU45_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5359050.1"
                     /translation="MTPTIDVSLLVPAKDEADNLPEFVRQCGEALGRAGFAFEVVIVN
                     DGSTDGTAGVLRELEAAHPFVRSVTHRRQRGIAEALRSAGEAAQGRVHVFYPADLQYL
                     PSDVPALVAPILSGSADIVTGTKQGKYEKAFVSSVYNVLCRWLFGVRVTDLNSVKAWR
                     REVMANVPFRPDWHRFMVVIAAAEGFRLTSVPVPLHPRRAGVSKFGWSRIPVGVLDLL
                     SVWFQLRFGRKPMLFFGVVGALLFLVGFLAGLVALVLRFGYGIGFRPLLNLVETMVIT
                     GTVLFAGGFVGEMVAGVREDVRELRRRQED"
     gene            complement(1270..2733)
                     /gene="rpoN"
                     /locus_tag="VFU45_08020"
     CDS             complement(1270..2733)
                     /gene="rpoN"
                     /locus_tag="VFU45_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEU5359051.1"
                     /translation="MKGGLSQHTSLRQELRINPRLYQAMDLLYMPMMDLQQHLKQELM
                     ANPFLELLEPEDEELPAETQAEQKEEEQKKEEMDWEEILLNGFDVGGAQSSAYEDREY
                     TEPVSVDQRDLDDHLRDQLDLLELSPRQRLLAEEFLGSIGDDGYLTGTVDEVVAGANE
                     LLAERTAAAEEAERTVPDVPPFTLAEGEEMLAIFQRLDPPGIGARDLRECILLQLADR
                     GLSDSLTYRLVAEAFAEVAAHKWADLARRFEVGPGEVQAAADQLTKLDPKPGLRYAAR
                     PDGYVIPDLIVDKVDGRYRVTLNDSGVPRLRLSRAYQEVARDKKGMDAEQKEFISSKL
                     NSAQWMIQAIEQRRQTMLKVMNFIVDRQREFFEKGVEYLKPLTLREVADVIDMHESTV
                     SRVTNDKYAQTPRGVFPLKYFFSSGLGTTSGEDASARAIRAKLQKMVAEENASDPLTD
                     QQIVHQFQEQGVQIARRTVAKYRDQLGILPARMRKRV"
     gene            complement(2730..3887)
                     /gene="lptB"
                     /locus_tag="VFU45_08025"
     CDS             complement(2730..3887)
                     /gene="lptB"
                     /locus_tag="VFU45_08025"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEU5359052.1"
                     /translation="MSEFRLDGLVGSEPALAVALEALVHTAGDDGRAPLGELVTAFRT
                     AYASADPEGGVAQLDLSGDQIRENLGQSVLPRLVEAGWITWDPELQAVAAAPWWWSAA
                     IADRNGTATRFRLAARSGFHRDSGGAPVVVERPSVLGGRGLMKSFKRRAVVNDVSVEV
                     RQGEIVGLLGPNGAGKTTTFYLLTGLILADAGVVTLDGRDITRAPMYERARAGLGYLA
                     QEPSIFRRLTVEENVMAVLETLPLPAAERQRRLETMLDELSIKHLRHSKAYALSGGER
                     RRLEITRALVSGPKFMLLDEPFAGVDPIAVHDIQTIVAGLRHRGIGVLITDHNVEQTL
                     DIVDRAYIMFEGKVQVEGSVRDLVFDDRVAQLYFGPTLTGRLRARLTERVG"
     gene            complement(3884..5248)
                     /locus_tag="VFU45_08030"
     CDS             complement(3884..5248)
                     /locus_tag="VFU45_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359053.1"
                     /translation="MKRAALLFAGLGAGAAPLAGQVPATPGSRCLLSIDHVSRATHQV
                     PNPDGTSNYFLGGDVRLSCQGTDVRMAADSVAAFNDLKVTYFLGHVRYDDSSITMTAD
                     NGTYYKDGERWEARGNVVTRNTRNGSTLRGPALDYLRAITGVRDTAEMYATGRPRVEY
                     VPTDSAGAHQEPYVIYADRVRLRGNDEVFGGGSVTVDRSDLSAKGDSLRLDTGPGQDG
                     GLYGRTEGRAEIDGKGATTFTLTGKRIDLTLTKNELTGVRSTGRAHAVDPDWDLLADT
                     IALGLDRQRLVETYAWGDSLRPRAVSPGRDIVADSLAIDTPDQALKQARAFGRAWVGG
                     AVDSVTHERDQLWGDTVVADFVRHDSAGKARSVISRVEARHDARSYHLLAPSGTCPGS
                     PVSYIRGGRIVLLFVPGGNDLDRVEVHGKVDGVQIEPVCAPPDSAKADSLKADSLRAA
                     RTGR"
     gene            complement(5248..5799)
                     /gene="lptC"
                     /locus_tag="VFU45_08035"
     CDS             complement(5248..5799)
                     /gene="lptC"
                     /locus_tag="VFU45_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEU5359054.1"
                     /translation="MRCFPAAVAFAAAVALAACNHNELRSTTTVAAADTADQVLVHLQ
                     HYITKAGVRQSLVEADTAYYYEATRRFELRHVKVTFYSATGTQSSVLTAREGTYYMQD
                     QNMEARGNVVVVSTDGRTLQSEKLRYDQATNKLSTDIAFTFSGPTASGSGNGFVSDPD
                     FRDVIIQQGRGIQRNGAFVPPGQ"
     gene            complement(5796..6752)
                     /locus_tag="VFU45_08040"
     CDS             complement(5796..6752)
                     /locus_tag="VFU45_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEU5359055.1"
                     /translation="MGEDALVAEARRVLELEARELRAAATRIGASFARAVGVIRAAKG
                     RVIVSGVGKSGLIARKIAATLTSTGTPAAFLHPVDSLHGDLGIVGPADVAILLSKSGN
                     TEELFGLVAALQRMNVPIIAITGGVDSLLAKAATVVLDAAVSEEACPHDLAPTTSTTV
                     ALALGDALAVALLGLAGFRSADFAALHPGGSLGRRLHLRVRDVMVTALQPLPSGATMR
                     DAVVALAGQRGLAVVTDGATLRGVLTTGDLTRLAERQPDFLGVSVAAVMTATPRTAEA
                     DELAAAAVGRMEQFGIMALPVLDGGRLVGVVHLHDLLRAGAV"
     gene            complement(6745..7302)
                     /locus_tag="VFU45_08045"
     CDS             complement(6745..7302)
                     /locus_tag="VFU45_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU5359056.1"
                     /translation="MLDPGRARAVRLFGFDVDGVLTDNGIYVAVEAGRRVETKRFHAL
                     DGVAFHFLRQAGIDVAWISGRTSEATVERARELQVAEVIQDTGLGFKLPAVRELLRRK
                     GIDWGQFAYVGDDLADIPVLRRAGLPLAVANAMPEVKALAAWTGQRTGGHGAVRELVE
                     AFLRARGEWDGLVARYLEERGDRDG"
     gene            complement(7295..8110)
                     /gene="kdsA"
                     /locus_tag="VFU45_08050"
     CDS             complement(7295..8110)
                     /gene="kdsA"
                     /locus_tag="VFU45_08050"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:TIGR01362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEU5359057.1"
                     /translation="MSGWTFGPGPFILAGPCVIEGGDVLPRVADELARLAQKLNVPAC
                     FKASFDKANRARASGARGPGLDEGLRVLENVRRASGLPVLTDIHEPGQAAPAAAVVDV
                     LQIPAFLCRQTDLLLAAGRTGKPVNVKKGQWMAAEGMAGAVEKVRQGGSTEVAVTERG
                     TFFGYGDLVVDMRSFLRMRQATGVPVIFDATHSVQQPGQGQGGTSGGLREFIPALLAG
                     AAVAGADGFFLEAHPDPARAPSDAATQWPLDRLEWLLTRTLQLQARARESQGA"
     gene            complement(8107..>9177)
                     /locus_tag="VFU45_08055"
     CDS             complement(8107..>9177)
                     /locus_tag="VFU45_08055"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU5359058.1"
                     /translation="RELMQIGIQPDILICRSEQPISADIKRKIALFCNVDFGCVIESP
                     DVRSIYEIPLRFSEQGFDEVVCERLRLTTKAPDLAPWRAIVDKVLRPASKLRIAVVGK
                     YTDLHDAYKSVHEALIHGGIAHDVGVNIEWLSSDAFTDQAAAGTMLQGFDGLLVPGGF
                     GVRGVDGMIEAIRWAREHELPFFGICLGLQVAIIEFARDVCGMTGTSSTEFEPECESP
                     VIALMQSQRDVKDMGGTMRLGAYAAKLRAGSRAAQAYGTLEISERHRHRWEVNNAYRD
                     VLAEFGLRLSGQSPDGGLVEVIELPDHPWFLGCQFHPELKSRPTRPHPLFAGFIGAAK
                     AHHARAQVPAEQPAAKLSGAGR"
BASE COUNT         1312 a         3372 c         3146 g         1347 t
ORIGIN      
        1 gaaccatccg gaaggaaggc gacccctgtc ggcgcatagc ctgtcgtcgc cgcactatcg
       61 acaatggtgc cgaggtcgac ccgcgccagg agtgcgaggt tcagccgggt gacatagcct
      121 tggccggtcg acgcgatcgc cacgccgtac ggctggcccg tcaatacatg ggccgtgacc
      181 aactcagggg acgaattgac cccggtggtc ggtcccgtgc tcccattggg gctgcaagcc
      241 tgggccagga ccagggccgt gaccgcgacc ccggcgtaca cgcgtctgtg gatcatgtca
      301 gcaactcctc tcagggggag agtcgacttc aaggcaccac ggcagagctc cgtcagtcct
      361 cctgccgccg ccgcagctcc cgcacgtcct cccgcacccc cgccaccatc tcgcccacga
      421 acccgccggc gaagagcacc gtcccggtga tgaccatcgt ctcgaccagg ttaaggagcg
      481 gccggaaccc gatcccgtag ccgaaccgca gcaccagcgc caccaatccg gccaggaacc
      541 ccacgaggaa gagcagcgcg cccacgacgc cgaagaagag catgggcttc cgaccgaagc
      601 ggagctggaa ccagaccgag agcaggtcga ggaccccgac ggggatccgg gaccagccga
      661 acttggagac acccgcccgc cgggggtgga gcggcaccgg caccgacgtc agccggaacc
      721 cctccgccgc ggcgatgacc accatgaagc ggtgccagtc ggggcggaac ggcacgttgg
      781 ccatgacctc gcggcgccac gccttgaccg agttgaggtc ggtgactcgc acgccgaaga
      841 gccagcggca gaggacgttg tagacgctcg agacgaaggc cttctcgtat ttcccctgct
      901 tggtcccggt cacgatgtcg gcgctgccgc tcaggatcgg cgcgaccagc gccggcacgt
      961 ccgacgggag gtactgcagg tcggccggat agaagacatg gacccgcccc tgcgccgcct
     1021 cccccgccga ccggagcgcc tcggcgatcc cccgctgccg gcggtgggtc acgctccgca
     1081 cgaacggatg ggcggcctcg agctcgcgca gcaccccggc cgtcccgtcg gtcgacccat
     1141 cgttgacgat caccacctcg aaggcgaacc ccgcccgccc cagcgcttcc ccgcactggc
     1201 gcacgaactc cggcaggttg tccgcctcgt ccttggccgg gaccaggagg ctcacgtcga
     1261 tggtcggggt catacgcgct tcctcatccg cgcgggaagg atgccgagct ggtcgcggta
     1321 cttggccacg gtgcggcgcg cgatctgcac gccctgctcc tggaactggt gcacgatctg
     1381 ctggtcggtg agcgggtcgc tggcgttctc ctccgccacc atcttctgca gcttggcccg
     1441 gatggcccgg gcgctcgcgt cctcgcccga agtggtgccg agcccgctgg agaagaagta
     1501 cttgagcggg aagacgccgc gcggggtctg ggcgtacttg tcgttcgtca cccggctcac
     1561 cgtggactcg tgcatgtcga tcacgtccgc cacctcgcgg agggtcaggg gcttcaggta
     1621 ttcgacaccc ttctcgaaga actcccgctg ccggtcgacg atgaagttca tgaccttgag
     1681 catcgtctgc cggcgctgct cgatggcctg gatcatccat tgcgcactgt tgagcttgct
     1741 ggagatgaac tccttctgct cggcgtccat ccccttcttg tcgcgcgcca cttcctggta
     1801 ggcccggctc aggcggaggc gcggcacccc ggagtcgttg agcgtcacgc ggtagcgtcc
     1861 atccaccttg tcgacgatca ggtccgggat cacgtagccg tcggggcgtg ccgcgtaccg
     1921 aagccccggc ttggggtcga gcttggtgag ctggtcggcg gccgcctgca cctcaccggg
     1981 cccgacctcg aaccgtcggg ccaggtcggc ccacttgtgg gccgccacct ccgcaaaggc
     2041 ctccgccacc agccggtacg tcagggagtc gctgagcccg cggtcggcca gctggagcag
     2101 gatgcattcc cgcaggtccc gcgcaccgat cccgggtggg tcgagccgct ggaagatcgc
     2161 gagcatctcc tcgccctcgg ccagcgtgaa cggcggcaca tccggcaccg tgcgctcggc
     2221 ctcctccgca gcggcggtcc gctcggccag gagctcgttg gcgccggcca ccacctcgtc
     2281 gaccgtaccg gtgaggtagc cgtcatcgcc gatcgagccg aggaactcct ccgccagcag
     2341 ccgctggcgg ggagagagct ccaggaggtc gagctggtcg cgcaggtggt cgtcgaggtc
     2401 gcgctggtcg accgacaccg gttcggtgta ttcccgatcc tcatacgccg aggactgggc
     2461 gccgccgacg tcgaagccgt tgaggaggat ctcctcccag tccatctcct ccttcttctg
     2521 ctcctcttcc ttctgctcgg cctgcgtctc cgccggcaat tcctcgtcct cgggctcgag
     2581 cagctcgagg aacgggttgg ccatcagctc ctgcttcagg tgctgctgca ggtccatcat
     2641 cggcatgtac agcaggtcca tcgcctggta gaggcgcgga ttgatccgga gctcctgccg
     2701 gaggctcgta tgctgggaga gcccgccctt catccgaccc gctccgtgag ccgggcccgc
     2761 agccggccgg tcagcgtcgg gccgaagtag agctgggcca cccggtcgtc gaagaccagg
     2821 tcgcggaccg agccctccac ctggaccttc ccctcgaaca tgatgtaggc ccggtccacg
     2881 atgtccagcg tctgctcgac gttgtggtcg gtgatgagca cgccgatccc gcggtggcgg
     2941 agcccggcca cgatggtctg gatgtcatgg acggcgatcg ggtcgacccc ggcgaagggc
     3001 tcgtcgagga gcatgaactt ggggccgctc acgagcgccc gggtgatctc cagccggcgc
     3061 cgctcgccgc cggagagcgc gtacgccttg ctgtgccgca ggtgcttgat cgacagttcg
     3121 tcgagcatcg tctccaggcg ccgctgccgc tccgccgcgg ggagcggcag cgtctccagc
     3181 accgccatca cgttctcttc gaccgtcaac cgccggaaga tcgacggctc ctgcgccagg
     3241 tacccgaggc cggcgcgggc ccgctcgtac atcggcgccc gggtgatgtc ccgcccgtcg
     3301 agcgtcacca ccccggcatc ggccaggatg aggccggtga gcaggtagaa cgtcgtggtc
     3361 ttccccgccc cgttgggtcc cagcagcccc acgatctctc cctgccggac ctcgacgctc
     3421 acgtcgttga cgacggcgcg ccgcttgaac gacttcatca gcccccggcc gccgagcacg
     3481 ctgggccgct ccaccaccac cggcgcgccc ccgctgtcgc ggtggaaccc gctccgcgcc
     3541 gccagccgga accgggtggc cgtaccgttg cggtccgcga tcgcggcgga ccaccaccag
     3601 ggggcggccg cgaccgcctg gagctccggg tcccaggtga tccaccccgc ctcgaccagc
     3661 ctcggcagga ccgattgtcc gaggttctcg cggatctggt cgccgctcag gtcgagctgc
     3721 gcgacgccac cctccggatc cgccgaggcg tacgccgtcc ggaacgccgt caccagctcg
     3781 cccagcgggg cgcgcccgtc gtcgccggcg gtgtggacca gcgcctcgag cgccacggcg
     3841 agcgccggtt cgctgccgac caggccgtcg agccggaact cgctcatcgt cccgtcctcg
     3901 cggcccggag cgagtcggcc ttgagcgagt ccgccttggc cgaatcgggc ggcgcgcaga
     3961 ccggctcgat ctggacgccg tcgaccttgc cgtggacctc gacccggtcc aggtcgttcc
     4021 cgcccggcac gaagagcagg acgatccgcc ccccgcggat gtagctcacc ggcgaccctg
     4081 ggcatgtgcc gctgggcgcc aggaggtgat acgacctcgc atcgtggcgg gcctcgaccc
     4141 gggagatcac cgaccgggcc ttgccggcgg agtcgtgccg gacgaaatcc gccacgaccg
     4201 tatcgcccca gagctggtca cgctcgtggg tcaccgagtc caccgcgccg ccgacccagg
     4261 cccggccgaa ggcccgcgcc tgcttgagcg cctggtccgg cgtgtcgatg gccagcgaat
     4321 cggcgacgat gtcccgcccg ggggagaccg cccgcggccg gaggctgtcg ccccaggcgt
     4381 atgtctcgac gaggcgctgc cggtcgagcc cgagggcaat cgtgtcggcc agcaggtccc
     4441 agtcggggtc gacggcatgc gcgcgcccgg tcgaccggac gccggtgagc tcgttcttcg
     4501 tcagggtgag gtcgatccgc ttgccggtca gcgtgaacgt cgtggccccc ttgccatcga
     4561 tctcggcgcg cccctcggtg cgcccgtaga gcccaccatc ctggcccggc ccggtgtcga
     4621 ggcgcagcga gtcgcccttg gcggagaggt cggagcggtc gaccgtcacc gatccgcccc
     4681 cgaagacctc atcgttgccg cggagccgca ctcggtcggc gtagatcacg tagggttcct
     4741 ggtgtgcgcc cgccgagtcg gtcggcacgt actcgacgcg cgggcggccg gtggcgtaca
     4801 tctccgcggt atcccgcacg cccgtgatcg cgcgcaggta gtcgagcgcg gggccgcgca
     4861 gcgtcgatcc gttccgggtg ttgcgggtga ccacgttgcc gcgcgcctcc caccgttcgc
     4921 cgtccttgta atacgtgcca ttgtcggccg tcatggtgat gctcgagtcg tcgtagcgga
     4981 cgtgcccgag gaagtaggtc accttgaggt cattgaacgc cgccacactg tccgccgcca
     5041 tccgcacgtc ggtgccctgg cagctcaggc gcacgtcccc gccgaggaag tagttcgacg
     5101 tcccgtccgg gttcggcacc tggtgcgtgg cacgggacac gtggtcgatc gacaggaggc
     5161 aacggctccc gggggtcgcc ggcacctggc ccgcgagcgg cgccgccccg gccccgagcc
     5221 cggcaaagag cagcgccgcc cgtttcatca ttgccccggc gggacgaacg caccgttgcg
     5281 ctggatgccc cggccctgct ggatgatcac gtcccggaag tccgggtcgg agacgaaccc
     5341 gttgcccgag ccgctggccg tcggccccga gaacgtgaac gcgatgtcgg tggacagctt
     5401 gttcgtcgcc tggtcgtacc ggagtttctc gctctggagg gtgcgaccgt ccgtggacac
     5461 cacgaccacg ttgccgcgcg cctccatgtt ctggtcctgc atgtagtagg tgccctcgcg
     5521 cgccgtgagc accgacgact gggtcccggt ggcggaatag aacgtcacct tcacgtgccg
     5581 gagctcgaac cgacgcgtgg cttcgtagta gtacgccgtg tccgcttcca ccagcgattg
     5641 gcgcaccccc gccttggtga tgtaatgctg gaggtgcacc aggacctggt cggcggtgtc
     5701 ggcggcggcg accgtcgtgg tgctgcggag ctcgttgtgg ttgcaggccg ccagcgcgac
     5761 cgccgccgcg aacgcgaccg ccgccgggaa gcacctcaca ccgcccccgc acgcaggagg
     5821 tcgtgcaggt ggaccacgcc caccagccgt ccgccgtcga gcaccgggag cgccatgatc
     5881 ccgaactgct ccatccggcc gaccgccgcc gccgcgagct catccgcctc ggcggtccgg
     5941 ggcgtcgccg tcatgaccgc ggcgaccgac acgccaagga agtcgggctg ccgctccgcc
     6001 aagcgggtga ggtcgccggt cgtgagcacg ccgcggagcg tggccccgtc ggtcaccacc
     6061 gccagcccgc gctggccggc gagcgccacc acggcgtcgc gcatggtggc gccggacggg
     6121 agcggctgga gcgccgtcac catgacgtcc cggacccgga ggtgcaggcg gcgccccagg
     6181 ctcccgcccg gatgcagcgc cgcgaagtcg gccgagcgga agccggcgag accgagcagg
     6241 gccaccgcca gcgcgtcgcc gagggcgagc gccacggtcg tgctcgtggt gggcgcgagg
     6301 tcgtgcgggc aggcctcctc gctcacggcg gcgtccagga ccaccgtcgc cgccttggcg
     6361 aggagggagt cgacgccgcc ggtgatcgcg atgatcggca cgttcatccg ctggagcgcc
     6421 gccacgaggc cgaagagctc ctcggtgttg ccgctcttgc tgagcaggat ggcgacgtcg
     6481 gcggggccga cgatgccgag gtcgccgtgg aggctgtcga cgggatgcag gaaggcggcg
     6541 ggcgtgccgg tggacgtgag ggtcgcggcg atcttccggg cgatgagccc cgacttgccg
     6601 acgccggaga cgatgacgcg ccccttcgcc gcgcggatga cgccgacggc ccgggcaaag
     6661 gatgccccga tgcgcgtggc cgcggcgcgc agctcccgcg cctcgagctc gagcacccgc
     6721 cgggcctcgg cgaccagcgc gtcctcaccc atcgcggtcc cccctctcct cgagataccg
     6781 ggccacgagg ccgtcccatt cgccgcgcgc ccggaggaac gcctccacca gctcccggac
     6841 ggcgccgtga ccgccggtcc gctggccggt ccacgccgcc agcgccttca cctccggcat
     6901 cgcattggcc accgcgagcg ggagcccggc acggcgcagt accgggatgt cggccaggtc
     6961 gtcgccgacg taggcgaact ggccccagtc gatccccttc cgccgcagga gctcgcgcac
     7021 tgccggcagc ttgaatccga ggcccgtgtc ctggatgacc tcggccacct gcagttcgcg
     7081 ggcccgctcg accgtcgcct cggacgtccg gccgctgatc cacgccacgt cgatccccgc
     7141 ctgtcgcagg aaatggaacg ccacaccgtc gagcgcgtgg aagcgcttgg tctcgacccg
     7201 ccgcccggcc tcgaccgcga cgtagatccc gttgtcggtg aggaccccgt cgacgtcgaa
     7261 gccgaagagc cgcaccgcgc gcgcgcggcc cgggtcaagc accctgcgac tcccgggcgc
     7321 gggcctgcag ctggagcgtc cgcgtcagca gccactcgag ccgatccagc ggccactggg
     7381 tggcggcgtc gctcggggca cgcgccgggt cgggatgggc ctcgaggaag aagccgtcgg
     7441 cgcccgccac cgccgccccg gccaggagcg ccgggatgaa ctcccggagc ccgccgctcg
     7501 tccccccctg gccctgcccc ggctgctgca ccgagtgggt cgcatcgaag atcaccggca
     7561 cgccggtggc ctggcgcata cgcaagaaac tccgcatgtc gaccacgagg tcgccgtaac
     7621 cgaagaaggt cccgcgctcg gtgaccgcca cctccgtcga cccgccctgg cgcaccttct
     7681 cgaccgcgcc ggccatcccc tcggcggcca tccactgccc cttcttgacg ttgaccggct
     7741 ttccggtgcg cccggccgcg aggaggaggt cggtctggcg gcagaggaac gcggggatct
     7801 gcaggacgtc gaccaccgcg gcggcagggg ccgcctggcc cggctcgtgg atgtcggtga
     7861 ggaccggcag ccccgatgcc cggcggacgt tctccagcac gcgcaaccct tcgtcgaggc
     7921 cgggtccgcg cgctcccgac gcccgggccc ggttggcctt gtcgaagctc gccttgaaac
     7981 aggccgggac gttgagcttc tgcgccaagc gcgcgagctc gtcggcgacc cgcggcagca
     8041 cgtcaccgcc ctcgatcacg cagggaccgg ccaggatgaa cgggccgggc ccgaaggtcc
     8101 aaccgctcac cggcccgccc cgctcagctt ggcggccggc tgctcggccg ggacctgcgc
     8161 ccgcgcgtga tgggccttgg ccgcgccgat gaatcccgcg aagagcgggt gcggccgggt
     8221 cgggcgggac ttgagctccg gatggaactg gcaccccagg aaccacggat gatccggcag
     8281 ctcgatgacc tcgaccaggc cgccgtccgg cgactggccg gagagccgca ggccgaactc
     8341 cgccaggacg tcgcggtagg cgttgttgac ctcccagcgg tggcggtgcc gctcgctgat
     8401 ctcgagggtg ccgtaggcct gggcggcacg ggagcccgca cggagcttgg cggcgtacgc
     8461 gccgaggcgc atcgtgccac ccatgtcctt gacgtcgcgc tggctctgca tcagcgcgat
     8521 gaccggcgat tcgcactccg gctcgaactc ggtgctgctg gtgccggtca tcccgcagac
     8581 gtcgcgcgcg aactcgatga tggccacctg caggccgagg cagatgccga agaacgggag
     8641 ctcgtgctcc cgggcccagc ggatggcctc gatcatcccg tcgacgcccc gcacgccgaa
     8701 gccgccgggc accagcagcc cgtcgaaccc ctgcagcatc gtgccggcgg cggcctggtc
     8761 ggtgaaggcg tcgctcgaga gccactcgat gttcaccccg acgtcgtgcg cgatgccgcc
     8821 gtggatcagc gcctcgtgca ccgacttgta tgcgtcgtgc aggtcggtgt acttgccgac
     8881 caccgcgatg cgcagtttgg acgccgggcg cagcaccttg tccacgatgg cccgccacgg
     8941 cgccaggtca ggagccttgg tcgtgagccg caggcgttcg cagaccacct cgtcaaagcc
     9001 ctgctcgctg aagcggagcg ggatctcgta gatcgaccgc acgtcggggc tctcgatgac
     9061 gcagccgaag tccacgttgc agaacagcgc gatcttgcgc ttgatgtcgg cggagatggg
     9121 ttgctcgctc cggcagatca ggatgtcggg ctgaattccg atctgcatca gctcgcg
//
LOCUS       DASUAP010000301         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_133020, whole genome shotgun sequence.
ACCESSION   DASUAP010000301 DASUAP010000000
VERSION     DASUAP010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133020"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /gene="kdpC"
                     /locus_tag="VFU45_08060"
     CDS             complement(<1..516)
                     /gene="kdpC"
                     /locus_tag="VFU45_08060"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEU5359059.1"
                     /translation="MKQHLRPALVTLAGLTLITGVLYPGAVTLAASVLFPGRASGSIV
                     LRNGLPVGSRLIGQSFTDVGHFWGRPSATTPTPYDAEASSGSNLAISNPVLGDSIAAR
                     AARLRAADPGNPAPIPADLLTASASGLDPDISPEAAEYQVPRVARTRGVPAGLLRELV
                     AAATTGRTLGIF"
     gene            complement(527..>2588)
                     /gene="kdpB"
                     /locus_tag="VFU45_08065"
     CDS             complement(527..>2588)
                     /gene="kdpB"
                     /locus_tag="VFU45_08065"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEU5359060.1"
                     /translation="DATSPLFDATMVGRAMAESVRKLHPRRMLRNPVMFVVEVGSAFT
                     TILGIDALRGGGDAPAGFTLAISAWLWLTVLFANFAEALAEGRGQAQAAALRRAREGL
                     RARRLRRPEDHRDFEEVSAEELHRGDTVIVSAGETIPVDGDVIEGVASVDESAVTGES
                     APVIRESGGDRSAVTGGTRVLSDWLVIRVAAEPGHSFLDRMISLIEAARRQKTPNEIA
                     LDILLAGLTIVFLLATATLLPFSQYAVRVTGHGTPVSITVLVALLVCLIPTTIGGLLP
                     AIGIAGMDRMLQKNVIATSGRAVEAAGDVDVLLLDKTGTITLGNRRASAFLPAPGVTV
                     ERLGDAAQLASLADETPEGRSIVVLAKEAFGIRERDVHALGATFVPFTAQTRMSGVDL
                     AARRIRKGAAEAVGAWVRDQGGDVPASVTAAVDEVARSGGTPLLVTEGADVLGVVQLK
                     DIVKGGIRERFADLRRMGIRTVMVTGDNPVTAAAIAAEAGVDDFIAQATPERKLALIR
                     EYQAGGRLVAMTGDGTNDAPALAQADVAVAMHSGTQAAKEAGNLVDLDSDPTKLIAVV
                     ETGKQLLMTRGSLTTFSIANDVAKYFAIIPAAFATTYPALGALNVMHLASPASAVMSA
                     VIFNAVVIVGLIPLALRGVTYRALGAATVLRQNLLVYGLGGLVVPFVGIKLIDLALSA
                     LGLA"
BASE COUNT          373 a          917 c          944 g          354 t
ORIGIN      
        1 gaagatgccg agggtccggc ccgtcgtggc ggccgccacg agctcgcgca gtaagccggc
       61 aggcacgccc cgcgtccgcg ccacccgtgg tacctgatac tcggcggcct cgggggagat
      121 gtcggggtcg agcccgctgg cggatgcggt cagaaggtcg gcgggaatgg gcgccgggtt
      181 gcccggatcg gccgcgcgga gccgagcggc acgcgccgcg atggagtcgc cgaggacggg
      241 gttggagatg gcaagattgc tccccgagga ggcctcggcg tcatacggcg tcggtgtcgt
      301 ggccgatggg cgcccccaga agtgaccgac gtcggtgaac gattgcccga tcagccgcga
      361 tccgaccggc aggccgttgc ggagcacgat gcttccggat gcgcggcccg ggaacaggac
      421 gctggccgcc agggtcacgg cgccggggta gaggaccccg gtgatcaagg tcagtccggc
      481 cagggtcacg agggcggggc gcaggtgctg cttcacaggc cgctccttac gccaggccca
      541 gcgcggaaag cgccaggtcg atgagtttga tgccgacgaa cggcacgacc agcccgccga
      601 gcccgtacac cagcaggttc tggcggagta ccgtcgccgc gccgagcgcg cggtacgtca
      661 cgccgcggag tgccagcggg atgaggccca cgatcacgac ggcgttgaag atcaccgccg
      721 acatcacggc gctcgccggg gacgccaggt gcatcacgtt gagtgcgccg agcgcggggt
      781 aggtcgtggc gaaggcggcc gggatgatgg cgaagtactt cgcgacgtcg ttggcgatgc
      841 tgaaggtggt gagcgacccg cgggtcatca gcagctgctt cccggtctcg accacggcga
      901 tgagcttggt cgggtcggag tcgaggtcca ccaggttgcc ggcctccttg gcagcctggg
      961 tccccgagtg catcgcgacg gccacgtcgg cctgggccag cgccggggcg tcgttggtgc
     1021 cgtcgcccgt catcgcgacc agccgcccgc cggcctggta ctcccggatg agcgccagct
     1081 tccgctccgg cgtggcctgc gcgatgaaat cgtcgacccc cgcctcggcg gcgatcgccg
     1141 ccgcggtcac cgggttgtcg ccggtcacca tcacggtgcg gatccccatc cgccgcaggt
     1201 cggcaaagcg ctcgcggatc ccgcccttca cgatgtcctt gagctggacc acgccgagca
     1261 cgtcggcgcc ctcggtcacc aggagcggcg tgcctcctga ccgcgccacc tcgtccacgg
     1321 ccgcagtgac ggaggccggc acgtcgcccc cctggtcgcg gacccaggcg ccgacggcct
     1381 cggcggctcc cttccggatc cggcgtgccg ccaggtccac gccactcatc cgggtctgcg
     1441 ccgtgaacgg cacgaaggtg gcgccgaggg cgtggacgtc gcgctcccgg atgccgaacg
     1501 cttcctttgc caggaccacg atgctccgtc cctccggcgt ctcgtcggcg agcgaggcga
     1561 gctgggccgc gtcgccgagc cgctcgaccg tcaccccagg tgcgggcagg aacgccgagg
     1621 cgcgccggtt ccccagcgtg atggtccccg tcttgtcgag cagcagcacg tcgacgtcgc
     1681 cggccgcctc cacggcgcgc ccgctggtgg cgatcacgtt cttctggagc atccggtcca
     1741 tccctgcgat gccgatggcg gggaggaggc cgccgatggt cgtcgggatg aggcacacca
     1801 ggagcgccac cagcaccgtg atcgagaccg gcgtgccatg gccggtgacc cgcaccgcgt
     1861 attgggagaa tgggagcaag gtggccgtgg ccaggaggaa gacgatcgtg agcccggcaa
     1921 ggaggatgtc gagcgcgatc tcgttcggcg tcttctggcg ccgggcagcc tcgatgagcg
     1981 agatcatccg gtcgaggaac gaatgccccg gttccgcggc cacccggatg accagccagt
     2041 cggagagcac ccgggtgccg ccggtgacgg cgctccgatc gccgccgctc tcccggatga
     2101 ccggcgcgct ctccccggtc acggcgctct cgtccaccga ggccacaccc tcgatcacgt
     2161 caccgtcgac ggggatcgtc tcgcccgccg acacgatcac ggtgtcgccg cggtggagtt
     2221 cctcggcgga cacctcctcg aagtcgcggt ggtcctccgg gcgccggagc cgccgggccc
     2281 ggagtccttc gcgcgcccgc cgcagggccg ccgcctgcgc ctggccgcgg ccttccgcca
     2341 gcgcctcggc gaaattggcg aacaggaccg tgagccagag ccacgccgag atggcgaggg
     2401 tgaatccagc aggcgcatcg ccgccgccgc ggagcgcgtc gatcccgagg atggtggtga
     2461 aggcgctccc gacctcgacc acgaacatca ccgggttccg gagcatccgg cgcgggtgga
     2521 gcttgcgcac cgactccgcc atcgcgcgcc cgaccatcgt ggcgtcgaag agcgggctcg
     2581 tggcatcg
//
LOCUS       DASUAP010000302         5719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_540705, whole genome shotgun sequence.
ACCESSION   DASUAP010000302 DASUAP010000000
VERSION     DASUAP010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540705"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFU45_08070"
     CDS             complement(<1..247)
                     /locus_tag="VFU45_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEU5359061.1"
                     /translation="MRPRTEAAIAIGGFALAAALVAIAGSGRRGVLDQPFDPRSTYHP
                     GPNGARALADVLPRLGVAVYRYRRPSRELPDRVPGPLD"
     gene            complement(231..842)
                     /locus_tag="VFU45_08075"
     CDS             complement(231..842)
                     /locus_tag="VFU45_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEU5359062.1"
                     /translation="MGPVAPDSLRAALDSVFADRAYHWRGVTDPLGWLGRWFGALADW
                     IMGLRQGQPWLYWAVLLALLTLVVGIFVHVAWVAARAVRYSTAADASYAGAAAEVHDE
                     AWFLALADRLAGDGRSAEAVRTAFQALVLELTRAGVVRYHPSKTPREYLREARLPVEA
                     RARLGGLVESLYRFAFAGASCGPSDYAEWRRRAAEVRWDAPAH"
     gene            complement(842..1636)
                     /locus_tag="VFU45_08080"
     CDS             complement(842..1636)
                     /locus_tag="VFU45_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359063.1"
                     /translation="MTTVTLRPLAMGEILDQAFGLFRRKFGALLTISVVCSGLPALLN
                     VLVRARGGLILAWGLGLTAMVLGVIGGAVATGASTYVVSGAYLNHEVSATQALERALP
                     RLWQLILCSFGFGLLVGLGMLLLFVPGVIALSGCVLCFTAVVVERLDAQAALGRSWEL
                     TRGFRWRMLGLLFVFGVVLYVPMLAVGVVVGMMGGLDPAAIRAVQMGGTVTAGFLFVS
                     VVTAVVQIVLSPFLYCILVTAYYDLRVRKEAFDLELLAGSLAPAGA"
     gene            1712..3478
                     /locus_tag="VFU45_08085"
     CDS             1712..3478
                     /locus_tag="VFU45_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEU5359064.1"
                     /translation="MAATPASPPAPPDFRQHFSVETPEHVALEYEVAGIGSRILAAVV
                     DQLIIVGWGIAVAIIAGFIGGGPFVGALMLLLWGAGSLAYFVGFEAFRQGQTIGKRAN
                     GIRVVRETGHPVTFGAALARNVLRAADFLPVGYLVGLGFVMLHPRARRLGDLVAGTVV
                     VRDRPEAAPAPVAPEVPAHDVVVPSAGTRPQLTDDEYRLLSAFVTRRDLEPAVTSRVA
                     AGLVARLADRFPVRTQGDVAFLRDLHQVETVRRGSAPAAAQGTAVRFVARRSGRWAEF
                     QALAARAAERGLDSFAAAELPDFAARYREVAADLARARTYGVDPHAVARLERAVAAGH
                     NVLYRDERNTGARVLAVLFRECPAAIVRAAPAVLLALACLFGPAAAGWMLIRERPALA
                     YDVLPDVMIERAAAGHARLRQGLGYFEAPTGEQPVMAAEIINNNVRVAFSCFAGGIFV
                     GVGSLVLLGYNGLELGATAAHFANQGLLGYLLAFIIGHGVLELFAISLAGAAGFLLGR
                     AVIAPGDHPRGEAVALAGRTAVRLVGAVVVLLVVAGSIEGMASASDLGTGYRLGISGL
                     SVVFLALYLTNGWRHRRDAAGA"
     gene            complement(3588..3770)
                     /locus_tag="VFU45_08090"
     CDS             complement(3588..3770)
                     /locus_tag="VFU45_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU5359065.1"
                     /translation="MTDAERLHKLRHDIANPLSALLAEAQLLLLNDAKLDPEVAASLR
                     EIENLAIRMRALLRDV"
     gene            complement(3786..4076)
                     /locus_tag="VFU45_08095"
     CDS             complement(3786..4076)
                     /locus_tag="VFU45_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359066.1"
                     /translation="MPAQPARQPLREPREPSVHELPSFRFEGLTIEVRALVLRGEDGT
                     WRGRLRFLGGNDGPDAERETAEIFCAVSEAELWQSVRGLGSHHVRDLYRSLL"
     gene            4390..5637
                     /gene="rho"
                     /locus_tag="VFU45_08100"
     CDS             4390..5637
                     /gene="rho"
                     /locus_tag="VFU45_08100"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU5359067.1"
                     /translation="MDIAELKQKSVAELHALAEQLNIANYSGLRKQDLIFRIEQTLLD
                     TDTVIRGEGVLEILPEGYGFLRSQDWNYLYGPDDIYVSPSQIKRFDLRTGDTVMGQVR
                     PPKEGERYLALLKVESVNYEEPEKTKHRIAFDNLKPRYPDERISLERKTQDLSMRVVD
                     LLSPIGKGQRGLIVAPPKAGKTILMQKLANAISENHPEITLIVLLIDERPEEVTDMQE
                     NVKAEVISSTFDEPADRHVQVADMVIEKSKRLVEHGRDVVILLDSITRLARAHNVVVP
                     HSGKILSGGVDANALQKPKRFFGAARNIDKGGSLTIIATALIDTGSRMDEVIFEEFKG
                     TGNMELVLDRRLADRRIYPAIDIQRTSTRKEELLMDKDELNRVYLLRNFLADMPPVEA
                     LEFLLERMKRTKNNKEFFATMAQ"
BASE COUNT          897 a         2039 c         2025 g          758 t
ORIGIN      
        1 agtcgagggg cccgggcacg cggtcgggaa gttcgcggct cgggcgccgg tatcggtaca
       61 cggccacgcc gagtcgcggc agcacgtcgg cgagcgcgcg ggcgccgttg ggaccggggt
      121 ggtaggtcga ccggggatcg aatggctggt cgaggacgcc gcggcgcccg ctccccgcga
      181 tcgccaccag cgccgcggcg agcgcaaagc cgccgatggc gatcgccgcc tcagtgcgcg
      241 ggcgcatccc accgcacttc cgcggcccgg cggcgccact cggcgtagtc ggacggcccg
      301 cagctggcgc cggcaaaggc gaaccggtag agcgactcga ccagtccacc gaggcgggcg
      361 cgcgcctcga ccggaaggcg tgcctcgcgg aggtattcgc ggggcgtctt gctcgggtga
      421 tagcggacca cgccggcgcg ggtgagctcg aggaccagcg cctggaacgc cgtccggacc
      481 gcctcggcgg atcgcccgtc tcccgccagc cggtcggcca gcgccaggaa ccaggcctcg
      541 tcatggacct ccgcggcggc gcctgcgtag ctggcgtcgg cggcggtcga gtagcggacg
      601 gcccgcgccg cgacccacgc gacgtggacg aagatgccga ccaccagcgt cagcagggcg
      661 agcaggaccg cccagtacag ccacggctgt ccctgccgca gccccatgat ccagtcggcc
      721 agcgcgccga accagcgccc gagccatccc agcggatcgg tgaccccgcg ccagtggtac
      781 gcccggtcgg cgaagacgct gtcgagcgcg gcgcgaaggg aatcgggggc caccgggccc
      841 atcacgcgcc tgcgggcgcc agcgatcccg ccaggagctc gaggtcgaag gcctctttcc
      901 gcacgcgcag gtcgtaatag gcggtgacga ggatgcagta gaggaacggc gagagtacga
      961 tctgcaccac cgcggtgacg acgctgacga agaggaagcc ggcggtcacc gttccgccca
     1021 tctgcaccgc ccggatcgcc gccggatcga gcccgcccat catgccgacg accaccccga
     1081 ccgccagcat cggcacgtag agcaccacgc cgaagacgaa cagcagcccg agcatccgcc
     1141 agcggaatcc gcgggtgagc tcccacgacc ggccgagcgc cgcctgcgcg tccagccgct
     1201 cgaccaccac cgccgtgaag cagagcacgc atccggacag ggcgatgacc ccgggcacga
     1261 agagcagcag catcccgagg cccaccagga gcccgaaccc gaacgaacag aggatcagct
     1321 gccagaggcg gggcagcgcc cgctcgagcg cctgcgtcgc cgagacttcg tggttcaggt
     1381 atgcgcccga taccacgtag gtcgaggcgc cggtggcgac cgcgccgccg atcacaccca
     1441 gcaccatcgc cgtcagcccg agcccccacg ccaggatcag gccgccgcgg gcgcggacca
     1501 gtacgttgag cagcgccggc aggccggaac acaccaccga gatcgtgagc aacgcgccga
     1561 acttgcgacg gaagaggccg aacgcctgat cgaggatctc ccccatcgcg aggggacgca
     1621 gcgtgaccgt ggtcatcggg tgtggctccg gaggcgggtg gaaggaccgg tcgcgattga
     1681 ttcgccggcg gccggagcgt aaagttcgcc gatggcagcc accccggcca gcccccccgc
     1741 cccgccggac ttccggcagc acttctcggt cgagaccccg gaacacgtcg ccctcgagta
     1801 cgaggtcgcg gggatcgggt cgcgcatcct ggcggccgtc gtcgaccagc tcatcatcgt
     1861 cggatggggc atcgccgtcg cgatcatcgc cggcttcatc ggcggcggcc cgttcgtcgg
     1921 tgcgctgatg ctgctcctgt ggggcgccgg ctccctcgcc tatttcgtcg gcttcgaggc
     1981 gttccggcag ggccagacga tcggcaagcg ggccaacggg atccgcgtcg tccgggagac
     2041 gggccacccg gtgacctttg gcgccgcgct ggcgcggaac gtcctgcgcg ccgcggactt
     2101 cctgccggtc ggctatctcg tgggcctggg attcgtgatg ctccatccgc gggcccgccg
     2161 cctcggcgat ctcgtcgccg gtacggtcgt ggtgcgcgac cgcccggagg cggcgccggc
     2221 acccgtcgcc cccgaggtgc ctgcgcacga tgtcgtcgtg ccgtccgccg gaacacgccc
     2281 ccagctcacc gatgacgaat accggctcct ctccgccttc gtgacccgac gggacctgga
     2341 gcccgcggtg acctcgcggg tggcggccgg gctggtcgcg cggctggcgg atcgcttccc
     2401 ggtccgcacc cagggcgacg tggcgttcct ccgcgacctg caccaggtcg agaccgtgcg
     2461 ccgaggatcg gcgccggcgg cggcacaggg cacggcagtg cggttcgtcg cgcggcggag
     2521 cgggcgctgg gccgagttcc aggcgctggc ggctcgggcg gccgagcgcg ggctcgacag
     2581 cttcgcggca gcggagctcc cggacttcgc ggcccgttac cgcgaggtgg cagccgacct
     2641 cgcgcgcgcg cgcacctacg gcgtcgatcc gcacgcggtg gcccggctcg aacgcgcggt
     2701 ggccgccggc cacaacgtgc tctaccgcga cgagcggaac accggcgcgc gggtcctcgc
     2761 ggtgctcttc cgtgaatgtc ccgcggccat cgtgcgcgcc gcgcccgccg tcctccttgc
     2821 actggcgtgc ctgttcggcc cggcggcggc cggctggatg ctcatccggg agcgccccgc
     2881 gctggcgtat gatgtcctgc ccgacgtgat gatcgagcgt gcggcggcgg ggcacgcgcg
     2941 gctgcggcag gggctgggct acttcgaggc gccgacgggc gagcagcccg tgatggccgc
     3001 cgagatcatc aacaacaacg tgcgggtggc gttctcctgc ttcgcgggcg ggatcttcgt
     3061 gggcgtgggc tcgctggtgc tgctcggcta caacgggctc gagctcggcg ccaccgcggc
     3121 gcacttcgcc aaccagggac tgctgggata cctgctcgcg ttcatcatcg ggcacggcgt
     3181 gctcgagctg ttcgcgatct ccctcgccgg cgccgcgggc ttcctgctcg gccgggcggt
     3241 catcgccccg ggcgaccacc cccgcggcga ggccgtggca ctggccggac gcaccgcggt
     3301 ccgcctggtc ggcgcggtgg tggtgctgct cgtggtggcg gggtccatcg agggcatggc
     3361 gtcggcgagc gacctgggga caggctaccg gctggggatc agcggactga gcgtggtgtt
     3421 cctggcgctc tacctcacga acgggtggcg ccaccgccgg gacgcggccg gcgcctgagc
     3481 gcccggcgcc cgggccagat ccagcccggg gtgcgctccc ggccgcggca gccggggacc
     3541 ggccaccgca ccgaactgcg ccgcgccggg ctcgggcggc tcacccctca gacgtccctg
     3601 agcagcgcgc gcatccggat ggccaggttc tcgatctcgc ggaggctggc cgcgacctcg
     3661 ggatcgagct tggcgtcgtt gaggagcagc aattgggctt cggcaaggag cgccgagagg
     3721 gggttggcga tgtcgtggcg gagcttgtgg agacgttcag cgtcggtcat gccgggcccg
     3781 tccgtctaga ggagggagcg gtagaggtcg cgcacgtggt gggagccgag cccgcggacc
     3841 gactgccaca actccgcctc cgagacggcg cagaagatct cggcggtctc gcgttcggcg
     3901 tccgggccgt cgttgccgcc gaggaaccgg agccggccgc gccaggtgcc gtcctcgccc
     3961 cgcaagacga gggcgcggac ctcgatggtg aggccctcga aacggaacga tggcagttcg
     4021 tggacggagg gttcgcgggg ttcgcgcagg ggctgtcggg cgggctgagc aggcatctac
     4081 cgtccttgac ctgggggctg gtccggacac ctggccgggg cacgaaccgc agtcctaagc
     4141 tagggaaatc cttgcactta ggcaataggg cgccggtcgg acgagggggc ccgtcgcttg
     4201 acgggggggc cctcccaccc taccttatcc cccgcatacc gtgactcacc gttcccaatc
     4261 ccacacatcg tgacccaggc gccgcgctcc cgcggaccga caccgtttcc gaccttccct
     4321 tcccccacaa tcgacgcagt ggcccccggc cgaatgccgc cggtacccgc cccgcggagc
     4381 gtccgtcccg tggacatcgc agaactcaaa caaaagtccg tcgctgagct gcacgcgttg
     4441 gccgagcagc tcaacatcgc caattactcc gggctccgga aacaggacct gatcttccgg
     4501 atcgagcaga cgctgctcga tacggatacg gtcatccgcg gggagggcgt gctcgagatc
     4561 ctgcccgagg gctacggctt cctccgcagc caggactgga actacctgta cgggcccgac
     4621 gacatctacg tgagcccgag ccagatcaag cgcttcgacc tccgcaccgg ggacacggtg
     4681 atgggccagg tgcgcccgcc gaaggagggc gagcgctacc tcgcgctcct caaggtcgag
     4741 agcgtcaact acgaggagcc ggagaagacc aagcaccgga tcgcgttcga caacctgaag
     4801 ccgcgctacc ccgacgaacg gatcagcctc gagcggaaga cgcaggacct ctcgatgcgg
     4861 gtggtggacc tgctctcgcc catcggcaag ggccagcgcg ggctgatcgt ggcgccgccc
     4921 aaggcgggca agacgatcct gatgcagaag ctggccaacg ccatcagcga gaaccacccg
     4981 gagatcacgc tcatcgtact cctgatcgac gagcgccccg aggaagtgac cgacatgcag
     5041 gagaacgtga aggccgaggt catctcctcg accttcgacg agcccgccga ccggcacgtc
     5101 caggtggccg acatggtcat cgagaagtcg aagcggctgg tggagcatgg acgggacgtg
     5161 gtgatcctgc tcgactcgat cacccggctc gcgcgggccc acaacgtcgt ggtgccgcac
     5221 tccggcaaga tcctctcggg cggcgtcgac gccaacgccc tccagaagcc caagcggttc
     5281 ttcggcgcgg cccgcaacat cgacaagggc ggctcgctca cgatcatcgc cacggcgctc
     5341 atcgacaccg ggagccggat ggacgaggtc atcttcgagg agttcaaggg caccggcaac
     5401 atggagctcg tcctcgaccg ccgcctggcc gaccgccgca tctacccggc catcgacatc
     5461 cagcgcacca gcacccggaa ggaggagctc ctgatggaca aggacgagct caaccgggtg
     5521 tacctgctcc gcaacttcct ggccgacatg ccgcccgtcg aggccctgga gttcctgctc
     5581 gaacggatga agcgcacgaa gaacaacaag gagttcttcg ccacgatggc gcagtgatcg
     5641 gtgaacggtg aacggtcaac agggaacggg gactccgcac gacccggggt ccccgttcac
     5701 cgttgaccgt tgaccgttg
//
LOCUS       DASUAP010000303        11736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_609332, whole genome shotgun sequence.
ACCESSION   DASUAP010000303 DASUAP010000000
VERSION     DASUAP010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609332"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFU45_08105"
     CDS             <1..493
                     /locus_tag="VFU45_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359068.1"
                     /translation="DSSGSHYDFPLPVATSDTTGFTSVAAGGLHTCASGVSVTYCWGS
                     DRYAQIGNRTISATDDSLPTAMDSTLGLRSLTGDTASTVALGLGNKVYWWGTRGASLS
                     APVSADRSALPKAVPNYTFISIATGRGVSCGLLSNNFVFCWGSSADPNFPATDKPAGV
                     PAP"
     gene            646..2055
                     /locus_tag="VFU45_08110"
     CDS             646..2055
                     /locus_tag="VFU45_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359069.1"
                     /translation="MRRPNLVLISAGICVVAIGGCSADAVSPPSLGSVVLVSGDAQTS
                     SPGQALPDTLVARILSVTGRPMSGVEVSWRVTYGAGTLTPGPDTSDANGNIRATLTLG
                     PAPGTTQVVATVADLPGVTFTATGRGLQATSLAVGLVTACAIDITAHTWCWGGTASSG
                     DSGAATAPYYVPRLVAGGHHFTELAAGEEHMCGLDQSGTTWCWGWDYYQALGTGATGH
                     VDYPVMLASAPTFAHIFGGDAEHTCGLTADGTAYCWGRDDLGQLGSGSTTTSVGTPVA
                     VAGGIKFSSLSPTIDRTCGVAIGGAAYCWGNNNGVLLGDSGASGAASNVPVPVAGNHT
                     FLALATTDTYTCGVTSDVGRVCWGAVPDGGAQRGVPVPAPQLSDLSEYLPAYNGGFGI
                     LAKEAISLVGFSSPRNEGAPIPLHGLTAGFWTACALAQDATVYCWGVNDAGQAGNPDA
                     YPYDGGWSAAHAVVVPATY"
     gene            2112..2576
                     /locus_tag="VFU45_08115"
     CDS             2112..2576
                     /locus_tag="VFU45_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HEU5359070.1"
                     /translation="MPIARIISGGQTGVDRAALDVALVLGISCGGWCPRGRLAEDGVI
                     AARYPLYEAPSGDYAVRTEWNVRDADGTIALTKGEPEGGTRLTVEIAKRHGKPCLLID
                     LRNPPDPRVVARWARAHHIRTLNVAGPRASTVPSAYDDATGFLVKLFEILRR"
     gene            complement(2721..3143)
                     /locus_tag="VFU45_08120"
     CDS             complement(2721..3143)
                     /locus_tag="VFU45_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359071.1"
                     /translation="MTDPGLAGVAPAPAPSKWRHRLRVALALIVVVPILTLSLYTWSA
                     LHWSYSKGERSGVLQKFSEKGWICKTWEGELAMTTVPGTAPVLWNFTVRSDSIAAQVS
                     EMVGHEGRMVLTYEEHRGVPTKCFGDTRYYVVGVRMVK"
     gene            complement(3179..3967)
                     /locus_tag="VFU45_08125"
     CDS             complement(3179..3967)
                     /locus_tag="VFU45_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HEU5359072.1"
                     /translation="MPSAYLFDLDGTLYTDAGPVPGAVEVLEGLKHARIPFRYITNTS
                     RRPRAAIVERLRGYGFPASAEHVMTAVLAAAELVRHRGHRVVAPFVAPQTLPDLGEFE
                     LVGGTSGVRSGAGAPDAIVLGDLGDGWSYSLLQDAFRAVMGGADVIALSRDRYWLRGD
                     GLALDAGPFVAAIEYATGASARVAGKPSRDFFEAAIRSLGLGAGTSVADIAVVGDDVW
                     SDVKGAQGCGLLGWLVRTGKFRADALASSGVTPDRIIDSVAAIS"
     gene            complement(4064..4342)
                     /locus_tag="VFU45_08130"
     CDS             complement(4064..4342)
                     /locus_tag="VFU45_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359073.1"
                     /translation="MGHSPMTSSPPHLPNAPLARHLGDLTRNDTRWGVYLEARQQKDN
                     WAGRLHFVDGPRHRSTGWIFVEWAESALIDRFNEFSPVELWRLVESLH"
     gene            4459..5253
                     /locus_tag="VFU45_08135"
     CDS             4459..5253
                     /locus_tag="VFU45_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5359074.1"
                     /translation="MTTPTTSKCPHCGSAAQGKFCANCGQPLGPSSCVRCQAPLSPGA
                     RFCHRCGTAVTGAMVGGGRTERTAWIVAGVLALGLVGMVAWRVLAGVAMPARPDTGLA
                     GGGTGLSAGPGIPVGQAPDISNMTPKERFARLNDRVMQAAQQGDTSTVINFTPMALGA
                     YAQLPAPDIDDRYHAAILHAQVGDFVPALSLADTIARLAPGNLFAPLVRGTVADFQGD
                     ASGRIAAFKDFQRHYAAEIQKSRPEYRDHRALIDDFKRAADSAGVR"
     gene            5404..6243
                     /locus_tag="VFU45_08140"
     CDS             5404..6243
                     /locus_tag="VFU45_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEU5359075.1"
                     /translation="MHTIRVAHSPDSDDAFMFYALAEGKIDTGNLRYVHELQDIQSLN
                     ERALKAELEVTAVSVHAYAYLADKYAFLSSGSSMGDGYGPRLVALKPAPADPRAGLKG
                     LRVAIPGKLTTAYLALQLYQPDVTAVVVPFDQVQDAVRRGEADVALVIHEGQITFGDE
                     GFHLWEDLGAWWLRDTGLPLPLGGNVVRRDLGPEVTAQVARDLKASIVYALAHRRPAL
                     EHAKRFNRGIGDARTDEFVGMYVNDWTVDYGPRGRQAVQLLLDRAFEARLIPRRVVVD
                     FVG"
     gene            6315..7379
                     /locus_tag="VFU45_08145"
     CDS             6315..7379
                     /locus_tag="VFU45_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5359076.1"
                     /translation="MVDAAAPVAVLVVDDEEPIRNALRKFLTQQGYEVSLAGTASEAL
                     AVAKRQRLTAVLLDMRLPDRSGMDLLPDLLEVDADLAILMLTAVNDATTASLCIQRGA
                     TDYLTKPLDLDDLDSALRHALRRRGTRIEEQSLQHWLKEEVAVRSAELRRERAKLEQV
                     TTATLEALVNALEAKDEYLRGHSARVADLAALVAAEMGLDDEQVEQLRRAGRLHDIGK
                     IGISEQVLQKHGPLTPEEFTHVKEHVTIGSQILAPLTHLGDIIAFVRHHHEHWDGGGY
                     PDGLKGEQIPLGARILGVVEIYDALTTARPYQERMEPDAAVARMKALSARVIDPTAYD
                     ALVRAVERRRNLVFLDDPRH"
     gene            7409..8440
                     /locus_tag="VFU45_08150"
     CDS             7409..8440
                     /locus_tag="VFU45_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5359077.1"
                     /translation="MVDDDPDIVRVVTAVLAAAGHGSPAIAATAADALRADPADLVLL
                     DLQLPDAPGLDVLATLRARPAPPAIVVITAHGSEDAAAQALRLGADDYLVKDAALRDH
                     LPKVVERQRRNLALRDALAAAERDLVAAERLAAIGQVTVSLHHALNNPLMAASAEVEL
                     LLRGGGLTPDQREGLENVRASLARIAEAVRGAAEMQAAPVVDYLEGSMKMVDLAAAAP
                     SVRPASRGTALVIAADAALGRILGHLLGHAGFAVEQCDPKDCSARLGPGRPPVRAVVL
                     QAGAVEADRLPAQSATSPLLVVVGPAAGLRPFDGKADLLVTVPFDPGVVVQQVIARLE
                     GNGGGAAGA"
     gene            complement(8460..9488)
                     /locus_tag="VFU45_08155"
     CDS             complement(8460..9488)
                     /locus_tag="VFU45_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU5359078.1"
                     /translation="MADENLWAQQADNRRKSAWLVAGFILFFAWLGFGGDWVYYLHTR
                     ALPADQYHHVVPWFGILLTLVGAGLCWYAWNNGADRVLWATGAREVTDPATDQEKTLV
                     NVVQEMAIAASIPVPRVWIVPDPDPNAFATGNDPATASIAVTQGLLDTLNRDELQAVV
                     AHEMAHVRNLDVRLMTLLAAIVGAVALMSQGAGRWLGFGGGGRGRDDEGKGAGPLAIV
                     VLVLWVVSLIVAPIVTRLLAMAVSRKREFLADATGAQFTRNPLALASALEKLDAASAP
                     TRTIASGAAHLCIVDPAGSALNDQEGAAADFLGSHPPTRIRIARLRGMGYQQMKRDGV
                     PIPDAGAA"
     gene            complement(9495..10058)
                     /locus_tag="VFU45_08160"
     CDS             complement(9495..10058)
                     /locus_tag="VFU45_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5359079.1"
                     /translation="MGAIVLLVLLVVAGLWFVAAYNRLIALKNNVVNAWKQIDVQLKR
                     RHDLIPNLVNTVKGAMNFEKETLEAVTAARTRALGASGVRETAKAEGELTQALGRLFA
                     VSEAYPDLKATGNVAQLQEELASTENKITFARQAYNDLATEYNTAQQQFPTSFVAGFA
                     KAVPAELWEIEDAGERAVPNVDLSMGK"
     gene            complement(10144..10770)
                     /locus_tag="VFU45_08165"
     CDS             complement(10144..10770)
                     /locus_tag="VFU45_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5359080.1"
                     /translation="MTSATPPADPLATLRSYRTLAPWGLQDLAGLAGAILEASGVVPL
                     SGAAQARPTVRTIRFYMTRALVDKPEGRGTAAVYGYRHLLQVLAIKLRQMEGMTLEAI
                     VQEARGLTGDAVERRVAATIGPALPPPELLGWTSSLRGRSGRVAAEAQAAMGSGAAPA
                     TCRRIPAARGVELLVDASHPALRHPGAESALAEAVRAAVERLAPGTDR"
     gene            complement(10767..11156)
                     /locus_tag="VFU45_08170"
     CDS             complement(10767..11156)
                     /locus_tag="VFU45_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5359081.1"
                     /translation="MRVLVIDDEPVIRGVIQRLLARVGHEVLAAAGPDTALRLLADAP
                     VDAVLLDRRMPKLSGEALYLVLIHRWPYLKGRVILMSGAPLDLEGGLPAELGSCHFLG
                     KPFTVDQLLAVLGEIERQVEKEDRDTA"
     gene            complement(11160..>11736)
                     /locus_tag="VFU45_08175"
     CDS             complement(11160..>11736)
                     /locus_tag="VFU45_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU5359082.1"
                     /translation="PLVAYTSLAPETASVLLDLGRIGVRRAVFARFDDDPARLRAVLR
                     EEWALTAPHQVLQALHPLAERLPDRVRWALEAAVQSPGEAGVDDLARRAQVGRRTCER
                     LFARLELPAPSKVLQMARLLYAHRLLLDPGFTVEDVAQKLGYSRARSLQLQLRSMFGR
                     TAGEVRLSLSPEEALTLLWERLVGIHERMAG"
BASE COUNT         1753 a         4301 c         4034 g         1648 t
ORIGIN      
        1 cgactcgtcg gggagccact atgacttccc gctcccggtc gcgacgtccg acaccaccgg
       61 gttcacctcg gtggcggcag gcgggctgca cacctgtgcc agcggcgtct cggtgacgta
      121 ctgctggggc tccgacaggt acgcccagat cgggaaccgc accatcagcg ccaccgacga
      181 ctccctcccg accgcaatgg actcgacgct cgggctcagg tcgctcaccg gagatactgc
      241 gagcaccgtg gcgctcggcc tgggcaacaa ggtctactgg tggggcaccc ggggcgccag
      301 cctcagtgcc ccggtgtccg cggatcggtc ggcgctcccc aaggcggtgc cgaactacac
      361 cttcatctcc atcgcgaccg gtcgcggtgt cagctgcggg ctgctgagca acaacttcgt
      421 gttctgctgg gggtcctccg ccgacccgaa cttccccgcc accgacaagc cggcaggcgt
      481 tccggcgccg tgagcagctg atgctgggcg tcccgggccc gaagctcgtg gaccgtggcc
      541 tgacccccta gtgtgtgcct gttccccgga gcaacgtcac accggcaatc ccacataact
      601 gatccaccag gaccgatcgg ccccggttcc cccgctggag gtcgtatgcg tcgtcccaac
      661 ctcgtcctga tctcggccgg catctgcgtg gtcgccatcg gcggctgctc cgctgatgcg
      721 gtgagccccc cgtcgctggg ctcggtcgtg ctcgtttccg gcgacgccca gacgagctca
      781 cccggccaag cactccccga cacgcttgtg gccaggatac tcagtgtcac gggacggccg
      841 atgtcgggcg tggaggtcag ctggcgcgtc acgtacggcg ccggcacctt gactcccggc
      901 cccgacacct cggatgccaa cggcaatatc cgcgcgacac ttacgcttgg gcccgcccca
      961 ggcaccacgc aggtcgtggc gacggtggcc gaccttcccg gagtgacctt cacggcgacg
     1021 ggcaggggcc tccaggcgac gtccctggcg gtcggcctcg tcacggcgtg tgccatcgat
     1081 atcacggccc acacctggtg ctggggtggg accgcttcca gcggcgacag tggcgcggcg
     1141 acagcgccct attacgtgcc gcgcctcgtg gccggagggc atcacttcac ggagctcgcc
     1201 gcaggcgaag aacacatgtg cggactcgac caatcgggga ccacctggtg ctggggctgg
     1261 gactactacc aggctctcgg caccggcgcg accggccacg tggattaccc ggtgatgctg
     1321 gcaagcgcgc cgacatttgc gcacatcttc ggcggcgatg ctgaacatac ctgcggcctc
     1381 accgcagatg gcacggcgta ctgctggggc cgggacgatc ttggtcagct cggatcgggc
     1441 tcgacgacga catccgtcgg gactcccgtc gcggtcgcag gcgggatcaa gttcagctcg
     1501 ctgagcccca cgatcgaccg cacctgcggc gtggcgatcg gcggcgccgc gtattgctgg
     1561 gggaacaaca acggcgtact gctcggcgac agcggggcca gcggggcggc cagcaacgtc
     1621 ccagtgccgg tcgccgggaa tcacacgttc ctcgcgctcg cgacgacgga tacgtacacg
     1681 tgcggcgtca ccagcgacgt cggacgggtg tgctggggcg ccgtgcccga tggtggcgcg
     1741 cagcgcggcg tgccggtgcc ggccccccag ctctcggatc tcagcgagta cctgccggcc
     1801 tacaacggag gcttcggcat cctcgccaag gaggcgatct cgctcgtcgg attcagctcg
     1861 ccccggaacg agggcgcccc gatcccgctg cacggcctca ccgccggctt ctggaccgcg
     1921 tgcgcgctgg cacaggacgc caccgtgtac tgctggggcg tcaacgacgc ggggcaggcg
     1981 ggcaaccccg acgcctaccc gtatgacggt ggctggtcgg ccgcacacgc cgttgtagtg
     2041 ccggcgacct actgacagcg ccgctcgagc ccgtccgcgc cccgcccttg cggggcgcgc
     2101 tactattcgg cgtgcccatc gcccgcatca tctccggcgg ccagaccggc gtcgaccgcg
     2161 ccgcgctcga cgtcgccctc gtgctgggca tctcctgcgg cggctggtgt ccccgcggcc
     2221 gcctggccga ggacggcgtc atcgccgcac gatatccgtt gtatgaggcg ccgagcgggg
     2281 attacgcggt ccggaccgag tggaacgtcc gcgacgccga cggcaccatc gccctcacca
     2341 agggagaacc cgaagggggc acccgcctta cggtcgagat cgcgaagcgc cacggcaagc
     2401 cctgcctcct catcgacctc cgcaacccgc ctgacccgcg cgtcgtggcc cgctgggccc
     2461 gcgcccacca catccgcacc ctcaacgtcg ccggcccgcg ggccagcacg gtcccctcgg
     2521 cgtacgacga cgcgacggga ttcctggtca agctgttcga gatattgaga aggtgagaag
     2581 gtgagaggtg agaggtgaga ggtgagaggt gagaggtgag aggtgagagg ttccgacgaa
     2641 tgccatgagc gttgaaatgc gcaaggggcc cgtgccgcca cggagccccc tcttacgtct
     2701 cacctcttac gtctcacgcg ctacttcacc atccgcactc ccacgacgta ataccgcgtg
     2761 tcgccgaagc acttggtcgg gacgccgcgg tgttcctcgt acgtcagcac catccgcccc
     2821 tcgtgcccca ccatctcgga gacctgcgcc gcgatcgaat cgctgcgcac cgtgaagttc
     2881 cagaggaccg gcgcggtgcc cggcacggtg gtcatcgcga gctccccttc ccaggtctta
     2941 cagatccacc ccttctcgga gaacttctgc agcacgccgg aacgctcccc cttggaatag
     3001 ctccaatgaa gggcggacca ggtgtagagg gacagggtaa ggatcgggac gacgacgatg
     3061 agcgccagcg ccacccgaag acggtggcgc cacttcgatg gcgccggggc cggggcgacg
     3121 ccggccaggc ctggatcggt catggcagga ggttaacgcc gcgcgccgcg gggcgccatc
     3181 aggagatggc cgccaccgaa tcgatgatac ggtcgggtgt cacgccgctc gaagcgagcg
     3241 cgtcggcgcg gaacttcccg gtccgcacca gccagccgag gaggccgcac ccctgcgcgc
     3301 ccttgacgtc cgaccagacg tcatcgccca cgacggcgat gtcggccacc gacgtgccgg
     3361 cgccgagccc gagggagcgg atggccgcct cgaagaagtc ccggctcggc tttcccgcca
     3421 ctcgcgccga cgcgcccgtg gcgtactcga tggccgccac gaacgggccg gcatcgagcg
     3481 cgagcccgtc cccgcggagc cagtagcggt cgcgcgagag agcgatcacg tccgccccgc
     3541 ccatcaccgc ccggaacgcg tcctggagga gcgagtagct ccagccgtcg cccaggtcgc
     3601 cgagcacgat cgcgtcggga gcgcctgcgc cggaacgcac gccggacgtg ccccccacga
     3661 gctcgaattc cccgaggtcg gggagcgtct gcggggccac gaacggcgcc accacccggt
     3721 gcccccggtg gcgcaccagt tccgcggccg cgagcactgc cgtcatcacg tgctctgccg
     3781 aggccgggaa gccgtacccc cgcaggcgct cgacgatggc ggcgcgaggg cggcgggagg
     3841 tgttggtgat gtagcggaac gggatgcgag cgtgcttcag gccttccagc acctcgaccg
     3901 cgcccggcac cggtccggcg tcggtgtaga gcgttccgtc gaggtcgaag aggtaggcgg
     3961 agggcatggg cgatgggacg gcgtgaacgg tgaacagtca acggtgaacg gtggacaggg
     4021 gggactcgaa cttcccgttc accgttgccc gttcactgtt cactcagtgg aggctctcca
     4081 ccaaccgcca cagctcgacc gggctgaact cgttgaaccg gtcgatcagg gcggattcgg
     4141 cccattcgac gaagatccag ccggtggagc ggtggcgggg accgtcgacg aagtgcaggc
     4201 gccccgccca gttgtccttc tgctggcgcg cctcgaggta gaccccccac cgggtgtcgt
     4261 tccgggtgag gtcgcccagg tggcgggcga gcggcgcgtt cggcaggtgt ggcggggaag
     4321 atgtcatagg ggagtggccc atcgcgcctc cgggtggata cacgctaacg ccgccgggca
     4381 ggcggcgcca gcgtcccgcc gctcccgggc agctccgggc cggtatacat tgcgcccgca
     4441 catcgacttg gacgaccgat gaccacgccg acgaccagca agtgccccca ttgcggcagc
     4501 gccgcccagg ggaagttctg cgccaattgc ggccagccgc tcgggccgag ttcgtgcgtt
     4561 cggtgccagg cccccctgtc gccgggcgcc cgcttctgcc accgctgcgg caccgcggtg
     4621 accggcgcca tggtcggcgg cggccgcacc gagcgcaccg cctggatcgt ggccggcgtc
     4681 ctggcgctcg gcctggtcgg gatggtggcc tggcgggtgc tggccggcgt ggcgatgccg
     4741 gccaggccgg acacgggcct cgccggcggc ggcacgggcc tgagcgccgg acccggcatc
     4801 cctgtggggc aggctccgga catcagcaac atgaccccca aggagcggtt cgcgcggctg
     4861 aatgaccgcg tgatgcaggc cgcccagcag ggcgatacgt cgaccgtcat caacttcacc
     4921 ccgatggcgc tcggcgctta cgcccagctt cccgcgcccg acatcgacga ccgctaccac
     4981 gccgccatcc tgcacgccca ggtcggcgac ttcgttccgg cgctctcgct ggcggacacc
     5041 atcgccaggc tggcccccgg gaacctcttc gcgccgctcg tccgcgggac cgtcgcggat
     5101 tttcagggtg acgccagcgg ccgcatcgcc gcattcaagg acttccagcg ccactacgcc
     5161 gccgagatcc agaaatcgcg ccccgagtac agggaccatc gtgcgctgat cgacgatttc
     5221 aagcgcgcgg cggacagcgc gggggtgcgg taagacgcag gggcgcatag gcgcataagg
     5281 cggatgaggc ggacggggcc gtgcgcaata gggacccgcg acctgtgacc cgcgacctgt
     5341 gacccgcgac ccgcgacccg cgacccgcga ccaccgacta acgaccaacg actcacgacc
     5401 ctaatgcaca ccatccgcgt tgcccattcg cccgactccg acgatgcgtt catgttctac
     5461 gcgctcgccg agggcaagat cgacaccgga aacctccgct acgtccacga actgcaggac
     5521 atccagtcgc tcaacgagcg ggcgctcaag gccgagctcg aggtgacggc ggtgtcggtc
     5581 cacgcgtacg cctatctcgc cgacaagtac gccttcctct ccagcggttc ctcgatgggg
     5641 gatggctacg ggccgcggct ggtggcgctc aagccggcgc ccgccgaccc gcgtgcaggg
     5701 ctcaagggcc tgcgggtcgc gatccccggc aagctcacca ccgcgtacct cgcgctgcag
     5761 ctctaccagc ccgacgtcac cgccgtcgtg gtgccgttcg accaggtgca ggacgcggtc
     5821 cggcgggggg aggccgatgt ggcgctggtc atccatgaag ggcagatcac cttcggggat
     5881 gagggcttcc acctctggga ggacctgggc gcctggtggc ttcgcgacac cggactcccg
     5941 ctgcccttgg gcggcaacgt ggtccgtcgc gacctcggcc ccgaggtcac cgcacaggtg
     6001 gcccgcgacc tcaaggccag catcgtgtac gccctggccc atcgccggcc ggcgctcgaa
     6061 cacgccaagc gcttcaatcg cggcatcggg gacgcacgga ccgacgagtt cgtggggatg
     6121 tacgtcaatg attggaccgt cgactacggc cctcgcgggc gccaggcggt ccagctcctg
     6181 ctcgaccgcg cgttcgaggc gcgcctcatc ccccggcgag tggtggtgga cttcgtgggc
     6241 tgatcccggt ccgcgattga cgccccccac ggcggccggt agcttcgcag gggccatccc
     6301 atcggaggga attcgtggtc gacgccgcag cgcccgtcgc ggtcctcgtc gtggacgacg
     6361 aagaaccgat ccgcaacgcg ctcaggaagt tcctcaccca gcagggctac gaggtctcgc
     6421 tcgccggcac cgcgtccgag gcgctggcgg tggccaagcg ccagcggctg acggccgtgc
     6481 tcctcgacat gcggctcccc gaccggagcg ggatggacct ccttcccgac ctcctcgagg
     6541 tcgacgccga cctcgcgatc ctgatgctca ccgcggtcaa cgacgccacc accgcgtcgc
     6601 tctgcatcca gcgcggcgcc accgactacc tcaccaagcc gctcgacctc gacgacctcg
     6661 acagcgccct ccgccacgcg ctccggcgcc gcggcacccg gatcgaggaa cagagcctgc
     6721 agcactggct caaggaagag gtggcggtgc ggagcgccga actccggcgc gagcgggcca
     6781 agctcgagca ggtcaccacc gccacgctgg aggcgctggt caacgccctc gaggccaagg
     6841 acgagtacct ccgcggccac tccgcgcgcg tggccgacct ggcggcgctg gtggccgccg
     6901 agatgggcct cgacgacgag caggtcgagc agctccgccg cgccggccgg ctccacgaca
     6961 tcggcaagat cgggatcagc gagcaggtcc tccagaagca cgggccgctc acgccggagg
     7021 agttcaccca cgtcaaggag cacgtgacga tcggctccca gatcctcgcg ccgctcaccc
     7081 acctgggcga catcatcgcc ttcgtccgcc accaccacga acactgggac ggcggcggct
     7141 atcccgacgg cctcaagggc gagcagatcc ccctcggcgc ccggatcctc ggcgtcgtcg
     7201 agatctacga cgccctcacc accgcccggc cctaccagga gcgcatggag cccgatgccg
     7261 cggtggcgcg gatgaaggcg ctctcggcgc gggtcatcga ccccacggcg tacgacgcgc
     7321 tggtccgggc ggtcgaacgc cggcgcaacc tggtcttcct cgacgacccc cgccactgac
     7381 ccctgaccgc ccgcccatcc ggatcctcgt ggtcgatgac gacccggaca tcgtccgggt
     7441 ggtcacggcc gtgctcgcgg ccgcgggcca cgggtccccg gcgatcgcgg cgaccgccgc
     7501 ggacgcgctg agggccgacc ccgccgacct cgttctcctc gacctccagc tccccgatgc
     7561 gcccggcctc gacgtgctgg ccacgttgcg ggcgcggccg gcgccccccg cgatcgtcgt
     7621 gatcaccgcc cacggcagcg aagacgcggc agcccaggcc ctccggctcg gcgccgacga
     7681 ctacctcgtc aaggacgccg cgctccgcga ccacctgccc aaggtggtcg agcgccagcg
     7741 ccggaacctg gcgctccgcg acgcgctggc cgcggccgag cgggacctcg tcgccgccga
     7801 acggctcgcc gccatcgggc aggtcacggt gtcgctgcac cacgccctca acaatccact
     7861 gatggccgcg agcgccgagg tggagctcct gctgcgggga ggcgggctca cgcccgatca
     7921 gcgggagggg ctcgagaacg tgcgcgcatc gctcgcgcgg atcgccgagg cggtgcgcgg
     7981 cgccgccgag atgcaggcgg cgccggtggt cgactacctc gagggctcga tgaagatggt
     8041 ggaccttgcc gcggcggcgc cgtcggtgcg gccagcgagc cggggcaccg ccctcgttat
     8101 tgctgccgac gccgcgctcg ggcgtatcct tggccacttg ctgggacacg ccggcttcgc
     8161 ggtggaacag tgcgatccga aggactgcag cgcccgcctc ggtccggggc gcccgccggt
     8221 gcgcgcggtg gtgcttcagg ccggcgcggt ggaggccgac cggctgcccg cgcagtcggc
     8281 gacaagcccg ctcctcgtgg tggttgggcc ggcggccggg ctccggccgt tcgacgggaa
     8341 ggccgacctc ctggtgacgg tccccttcga tccgggagtg gtagtgcagc aggtcattgc
     8401 gcgtctggag gggaacggcg gaggggcggc cggagcgtga ccggcgctga cggacggcct
     8461 caggccgccc cggcgtcggg gatcggcacg ccatcgcgct tcatctgctg gtagcccatg
     8521 ccgcgcagcc gggcgatccg gatccgggtc ggcgggtggg agccgaggaa gtcggccgcg
     8581 gcaccctcct ggtcgttgag cgcgctgccg gccggatcca cgatgcagag gtgggccgcg
     8641 ccgctcgcga tggtccgggt cggcgcgctc gcggcgtcga gcttttcgag cgccgaggcg
     8701 agcgccagcg ggttgcgggt gaactgggcc cccgtggcgt cggccaggaa ctcgcgcttg
     8761 cggctcacgg ccatcgcgag gaggcgggtg acgatcggcg ccacgatcag cgacaccacc
     8821 cagagcacga gcacgacgat ggcgagcggc cccgcaccct tgccttcatc gtcccgcccc
     8881 cggcccccgc cgccgaagcc gagccaccgc cccgccccct gcgacatcag cgccacagcg
     8941 cccacgatcg ccgcgagcaa ggtcatcagc cgcacgtcga ggttccgcac gtgcgccatc
     9001 tcgtgcgcca ccaccgcctg cagctcgtcg cgattgagcg tgtcgagcag tccctgcgtc
     9061 actgcgatgc tggcggtggc cgggtcgttg ccggtggcaa aggcattggg gtcggggtcg
     9121 ggcacgatcc acacccgcgg cacggggatg ctcgcggcga tggccatctc ctgcaccacg
     9181 ttgacgagcg tcttctcctg gtcggtggcg gggtcggtca cttcccgcgc accagtggcc
     9241 cagagcacgc ggtcggcgcc gttgttccac gcgtaccagc agaggcccgc gcccaccaat
     9301 gtgagcagga tcccgaacca gggcaccacg tgatggtact ggtcggcggg gagcgcccgg
     9361 gtgtggaggt agtagaccca gtcgccgccg aagccgagcc acgcgaagaa caggatgaac
     9421 cccgccacca gccacgccga tttccgtcgg ttgtcggcct gctgggccca gaggttctcg
     9481 tccgccatcc ccggctactt gcccatcgac aggtccacgt tcggcactgc ccgctcgccg
     9541 gcgtcctcga tctcccagag ctcggccggc accgccttgg caaagcccgc cacgaagctc
     9601 gtggggaact gctgctgcgc cgtgttgtac tcggtggcca ggtcgttgta cgcctgccga
     9661 gcgaacgtga tcttgttctc ggtgctggcg agctcctcct gcagctgcgc cacgttgccg
     9721 gtggccttga ggtcgggata ggcctccgac acggcgaaca gccggcccag cgcctgcgtg
     9781 agctcccctt ccgccttggc ggtctccctc acgccgctgg cgccgagcgc gcgggtgcgc
     9841 gcggcggtca cggcctcgag ggtctccttc tcgaaattca tcgccccctt gaccgtgttg
     9901 accaggttcg ggatgaggtc gtggcgccgc ttcagctgga cgtcgatctg cttccaggcg
     9961 ttgaccacgt tgttcttgag cgcgatgagc cggttgtacg cggcgacgaa ccagagtccg
    10021 gccacgacga gaaggacgag caggacgatc gcgcccatcg ggagctccgt gagtgtggga
    10081 gtattgtcgt caatctagcg gggagaacga cgctgtcacg cccggcccgg cgctgacggt
    10141 gggtcagcga tcggtgcccg gggccaggcg ttccaccgcg gcgcggacgg cctcggcgag
    10201 cgccgattcc gcaccagggt gccggagtgc cggatgcgac gcatccacca gcagctcgac
    10261 gccacgggcc gccgggatcc ggcggcaggt ggccggcgcc gcccccgatc ccatcgcggc
    10321 ctgcgcctcc gccgccaccc gccccgagcg tccgcggagg ctcgacgtcc atccgaggag
    10381 ctccggcggc ggaagggcgg ggccgatggt agccgccacc cggcgttcca ccgcgtcacc
    10441 cgtcaggccc cgcgcctcct gcacgatggc ctcgagggtc atcccctcca tctgccgcag
    10501 cttgatggcc agcacctgga ggaggtgtcg gtacccgtag accgccgcgg tgccccgtcc
    10561 ttccggcttg tccaccagcg cccgcgtcat gtagaagcgg atcgtgcgta ccgtggggcg
    10621 cgcctgcgca gcgccggaga gcggcacgac gcctgacgcc tcgaggatgg cgccggcgag
    10681 ccctgccagg tcctgcagtc cccagggagc gagcgtgcga tagctgcgga gggtggccag
    10741 cggatcggct ggcggggtgg cggaggtcac gccgtgtcgc ggtcctcttt ctcgacctgg
    10801 cgctcgatct cgcccagcac agccaggagc tggtcgaccg tgaacggctt gccgaggaag
    10861 tgacagctgc cgagctcggc ggggaggccc ccttcaaggt cgaggggcgc cccggacatc
    10921 aggatgaccc ggcccttgag gtagggccag cggtggatca gcacgaggta cagcgcctca
    10981 cccgagagct tgggcatccg gcggtcgagc agcaccgcat cgaccggcgc atcggccagc
    11041 aggcggagcg cggtgtccgg ccccgcggcc gccagcacct cgtggccgac acgcgccagg
    11101 agccgctgga taaccccgcg gatcacaggt tcatcgtcga tgacgagcac gcgcatcggt
    11161 caccccgcca tgcgttcgtg gattcccacc aggcgttccc acaggagcgt caacgcctct
    11221 tccggactca gcgacaaccg gacctccccg gcggtacggc cgaacatcga ccggagttgc
    11281 agttgcagtg agcgcgcccg cgaatacccc agtttctggg ccacgtcctc gaccgtgaag
    11341 ccgggatcga ggagcagccg gtgcgcgtac agcagccgcg ccatctgcag caccttgctc
    11401 ggcgccggca gctcgagccg ggcaaagagc cgctcgcagg tccggcggcc cacctgcgcg
    11461 cgccgcgcga ggtcgtcgac ccccgcctcc cccggcgact gtaccgccgc ttcgagcgcc
    11521 caccgcaccc ggtcggggag ccgctccgcc agcgggtgca gcgcctgcag tacctggtgc
    11581 ggcgccgtca gcgcccattc ctcgcgcagc accgcccgga gccgcgcggg atcgtcgtcg
    11641 aaccgggcga acaccgcccg ccgcaccccg atccgcccca ggtcaaggag caccgacgcc
    11701 gtttcggggg ccagcgaagt gtacgccacc agcggc
//
LOCUS       DASUAP010000304         4815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1086942, whole genome shotgun sequence.
ACCESSION   DASUAP010000304 DASUAP010000000
VERSION     DASUAP010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086942"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFU45_08180"
     CDS             complement(<1..711)
                     /locus_tag="VFU45_08180"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU5359083.1"
                     /translation="MSVPTRRHTPTVRVGSVSVGSAHPIVVQSMTNTDTADARATVDQ
                     VAALHRAGSELVRVTVNNDDAARAVPRIVEELDRLGLDVPVIGDFHYNGHLLLTKYPA
                     CAAALAKYRINPGNVGGKRHDDNFGAIIDVALEHGKPVRIGVNWGSLDQALLTQLMDE
                     NSALAEPRDARDVTMTAMVESAVRSAAFAEGRGMAHDRIILSAKVSGVQDLVDVYRML
                     AARADYPLHLGLTEAGLGA"
     gene            complement(768..1742)
                     /locus_tag="VFU45_08185"
     CDS             complement(768..1742)
                     /locus_tag="VFU45_08185"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU5359084.1"
                     /translation="MSAPGERLTRAAGIEVPLLCGAMYPCSNPELVAAVSEAGALGVV
                     QPISLTYVHGHEFRAGLRYIRRLTSRPIGMNALIEQSSRAYRERMERWIDVALEEGVR
                     FFVTSLGNPKWVVERVRPVGGVVYHDVTEARWARKGLDGGVDGLIAVNARAGGHAGGK
                     GAEELLDELRPFGVPVVCAGGVGDEHGFVRMLRLGYDGCQLGTRFIATTECTASNAYK
                     AAIVAADESDIVLSERLTGVPVAVINTPLIQRMGTRAGPLARWLLRGRRTKRWMRTAY
                     ALKSLWQLKRSLTGPVEYWQAGRSVQGIRSVEPVAAIVARFRAAAADG"
     gene            complement(1739..2215)
                     /locus_tag="VFU45_08190"
     CDS             complement(1739..2215)
                     /locus_tag="VFU45_08190"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEU5359085.1"
                     /translation="MAADAPQRLLGLWRHLAPLPGGRWFFSRLLRANVPYSGSIGARV
                     EVLEPGHARLSLADRRAVRNHLRSVHAVALVNLGELTSGLAMTTALPPGIRSIVTHIE
                     TEFVKKARGTLTAECRATPPQAVPAPVDQVVEAVIVDQGNDVVARVRVRWRLAPPA"
     gene            complement(2248..2862)
                     /locus_tag="VFU45_08195"
     CDS             complement(2248..2862)
                     /locus_tag="VFU45_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359086.1"
                     /translation="MRRSGWMLLAALVALSAPAVAQYPRGGMGGMGGRRGGERRRGEM
                     GSGRAFQDIPTDPEAEGPPDLQTLSLSADVDTTGLAAYGVAVKAYLDSTRTVRDSIWV
                     VLQRMRPGGDDATAMAPDPDGAGPGAGPGGGARRGGFEVLQRAWPALKKRDDAFVKSV
                     LKPALPKKEFKKWKKWHDDEVDQAKKDREDQFRQRMGGGGRGDR"
     gene            complement(2933..3748)
                     /locus_tag="VFU45_08200"
     CDS             complement(2933..3748)
                     /locus_tag="VFU45_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359087.1"
                     /translation="MRTHSMTIRLAGLAALAAFTSAPLQAQDVMAGTRWNVHQQDRDS
                     GVTWVFLEGGTLRVIAGGDSTSTDRWRWTDDSLEITLAHGGTFVGALAGQRLAGTRDV
                     GRPTEGWWYADQVGGPSVVPEEFAEATPSTDSVPADTAAAPQVQRRRGGMMRRRGQAG
                     GGASGGAGIRFGGLWAGGARLMIQFTLDGGARYVWQPSGGEPVRGQGTWRRVQRQVVV
                     RIKDASGADLVLRGEVTPRGFLGQATLPGGGTSEVILRRAPMRGTGRARSATD"
     gene            complement(3804..>4815)
                     /locus_tag="VFU45_08205"
     CDS             complement(3804..>4815)
                     /locus_tag="VFU45_08205"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5359088.1"
                     /translation="ERVLIATGSSPHRPPEVPFDGRVVHDSDTILSMDRVPRTMVVVG
                     GGVIGAEYASIFTALGVRVTLIEGRDRLLPFVDGEIADRLRRRLEGFGLRAVFNGRVA
                     QVAARDDGVTLTLAGGEVLAADIAFFAAGRQSNIAGLGLEALGVALGKRGLILVDEHY
                     ATSVPGMYAAGDVIGFPALASTSMEQARVAMVHAFSLAYKERMSPVIPLAVYTVPELG
                     QAGKTEEECRADGIDHLVGRAYYEKSPRGQIIGDLAGMLKLVFSPSDKRLLGVHIIGE
                     MASELVHVGAQVLAQQGTIDAFIEAVYNYPSLTDSYKYAAYDGLGALARRAAGDTGGT
                     AG"
BASE COUNT          676 a         1767 c         1708 g          664 t
ORIGIN      
        1 ggcgccaagc cccgcctcgg tcaggccgag gtggagggga tagtccgccc gggcggcgag
       61 catccggtag acatcgacca ggtcctgcac gcccgacacc ttggcgctca ggatgatccg
      121 gtcgtgcgcc atcccgcgcc cctcggcgaa ggcggccgag cgcaccgcgc tctcgaccat
      181 cgcggtcatc gtcacgtcac gcgcgtcgcg cggctcggcg agcgccgagt tctcgtccat
      241 cagctgggtg aggagcgcct ggtcgagcga cccccagttg acgccgatgc ggaccggttt
      301 cccgtgctcg agggccacgt cgatgatggc cccgaagttg tcgtcgtgcc gcttgccccc
      361 gacgttgccg ggattgatgc ggtacttggc gagcgccgcg gcgcaggcgg ggtacttggt
      421 gaggaggagg tggccgttgt agtggaagtc gccgatgacg gggacgtcga ggcccaggcg
      481 atcgagctcc tcgacgatcc ggggcaccgc gcgggcggca tcatcgttgt tgacggtgac
      541 ccggacgagt tcgctcccgg cgcggtggag ggcggcgacc tggtcgacgg tggcccgtgc
      601 gtcggcggtg tcggtgttcg tcatcgactg caccacgatc gggtgcgccg aaccgacgga
      661 gacgctgccg acgcggacgg tcggggtgtg acggcgcgtg gggacggaca tgggggaaat
      721 gtagcggcgg gcggcgggcg gcgggtagcg ggagagtgtg tgccgggtca cccgtccgcg
      781 gccgcggcgc ggaatcgggc cacgatcgcc gccacgggct cgacgctccg gatgccctgc
      841 acgctccgcc cggcctgcca gtactcgacg ggcccggtga gcgagcgctt gagctgccag
      901 agcgacttga gggcatacgc ggtccgcatc cagcgcttgg tgcggcgccc gcggagcagc
      961 caccgggcga gcggccccgc gcgggtgccc atccgctgga tgagcggggt gttgatcacg
     1021 gcgacgggga caccggtgag ccgctcgctc agcacgatgt cggattcatc cgcggcgacg
     1081 atggccgcct tgtacgcatt gctcgcggtg cactcggtgg tggcgatgaa gcgggtgccg
     1141 agctggcagc cgtcgtagcc gaggcggagc atccggacga agccgtgctc gtcgccgacg
     1201 ccgcccgcgc agaccaccgg cacaccgaag ggccggagct cgtcgagcag ctcctcggcc
     1261 cccttcccgc ccgcgtgccc gccggcgcgc gcgttgacgg cgatgagccc gtccacgccg
     1321 ccgtcgagcc ccttccgggc ccacctggcc tcggtgacgt cgtgatagac caccccaccc
     1381 acgggccgca cccgttccac cacccacttc gggttgccga gcgaggtcac gaagaaccgg
     1441 accccttcct cgagcgcgac gtcgatccag cgttccatcc gctcgcgata ggcgcgggag
     1501 gactgctcga tgagggcgtt catcccgatc ggccgcgagg tcaggcgccg gatgtaccgg
     1561 agcccggcgc ggaattcgtg gccatggacg taggtgaggg agatcggctg gaccacaccg
     1621 agcgcgcccg cctcggagac cgcggcgacg agctccgggt tgctgcacgg gtacatggca
     1681 ccgcacagga gcggcacctc gatgccggcc gcccgagtga gccgctcccc cggcgcactc
     1741 acgccggcgg ggccaggcgc cagcggacgc ggacccgggc cacgacgtcg ttcccctggt
     1801 ccacgatgac cgcctcgacg acctggtcca ctggcgccgg caccgcttgc ggcggggtgg
     1861 cccggcactc ggccgtcagg gtgccgcgcg ccttcttcac gaattcggtc tcgatgtggg
     1921 tcacaatgct gcggatgcct ggcggaagcg cggtggtcat cgccagtccg ctggtgagtt
     1981 cccccaggtt gaccaacgcg accgcgtgga ccgagcgcag atggttgcgc accgcgcgcc
     2041 gatcggcgag cgagagccgc gcgtggcccg gctcgaggac ctcgacccgg gcgccgatgc
     2101 tcccgctgta ggggacgttc gcgcggagga ggcgggaaaa gaaccaacgc ccgcccggga
     2161 gcggcgccag gtggcgccag agcccgagca gacgttgggg agcatcagcg gccatggcac
     2221 aaagctaggg ccgccacgag cgccgggcta ccggtcgccg cgaccgccac cgcccatccg
     2281 ctggcggaac tggtcctcgc ggtccttctt cgcctggtcc acctcgtcgt catgccactt
     2341 cttccacttc ttgaattcct tcttggggag cgccggcttg agcaccgact tcacgaacgc
     2401 gtcgtcgcgc ttcttgagcg cgggccacgc ccgctgcagc acctcgaagc cgccgcgccg
     2461 cgcgcccccg cccggaccgg caccgggtcc cgcgccatcg ggatcgggcg ccatcgcggt
     2521 cgcgtcatcg ccgccggggc gcatccgctg gagcaccacc cagatgctgt ctcgcacggt
     2581 gcgggtcgag tcgagatagg ccttcaccgc gacgccgtac gccgcgagcc cggtggtgtc
     2641 gacgtcggcc gagagcgaga gggtctgcag gtcgggcggg ccctccgctt cgggatcggt
     2701 cgggatatcc tggaacgcgc gaccgctgcc catctcgccg cggcggcgtt cgcccccccg
     2761 gcggccaccc atgccgccca tcccgcccct cgggtactgc gccaccgccg gcgcgctcag
     2821 cgccaccaac gcggccaaca gcatccatcc actgcgacgc atcacaactc ctcgatcagg
     2881 acgtagctgt tacgccggat gcgcccgggg tgctgagcgt gcggctcgga agtcagtcag
     2941 tggcggaccg ggctcgcccg gtgccacgca tgggggcccg ccgcaggatg acctccgagg
     3001 tgccgccccc cggcaacgtc gcctggccga ggaagccgcg cggcgtcacc tccccccgga
     3061 gcacgagatc ggcgccgctg gcgtccttga tccgcacgac gacctggcgc tggacccgcc
     3121 gccaggtgcc ctgcccgcga acgggctcgc ctccggaggg ctgccagacg taccgagctc
     3181 cgccgtcgag cgtgaactgg atcatcagcc tggccccgcc ggcccacagt ccaccgaagc
     3241 ggatcccggc acccccgctt gctccgcccc cggcctggcc gcgccgacgc atcatcccac
     3301 cccggcgtcg ctggacctgc ggcgccgcgg cggtgtcggc gggcacactg tcggtggaag
     3361 gcgtggcctc cgcgaattcc tccggtacca cgctcggccc gcccacctgg tcggcgtacc
     3421 accacccctc ggtcggccgc ccgacgtccc gcgtccccgc caggcgctgg ccggcgagtg
     3481 cgccgacgaa cgtcccgcca tgcgccagcg tgatctcgag ggaatcgtcc gtccagcgcc
     3541 acctgtcggt ggaggtgctg tcacccccgg cgatgacgcg cagcgtcccg ccctcgagga
     3601 agacccaggt gacgccggag tcgcgatcct gctggtggac gttccaccgg gtgccggcca
     3661 tcacgtcctg tgcctggagc ggcgcagagg tgaacgcggc cagggcggca aggccggcaa
     3721 gacggatcgt catggagtgg gtgcgcatgg tgtggtcggg cggggttcgg ggtccatcgg
     3781 ggagcgcggg ccaggatcac aacttacccg gcggtccctc cggtgtcacc ggcagcacgc
     3841 cgcgccaggg cgccgagccc gtcgtaggcc gcgtacttgt aggagtcggt caggctgggg
     3901 tagttgtaga ccgcctcgat gaaggcgtcg atcgtcccct gctgggccag cacctgcgcg
     3961 ccgacgtgca ccagctcact tgccatctcg ccgatgatgt ggacgcccag gagccgcttg
     4021 tcggacggcg aaaagacgag cttgagcatc ccggccaggt caccgatgat ctggccgcgg
     4081 gggctcttct cgtaatacgc ccgcccgacg agatggtcga tgccgtcggc ccggcactcc
     4141 tcctcggtct ttccggcctg gcccagctcc ggcacggtgt acaccgcgag cgggatgacc
     4201 ggggacatcc gctccttgta ggcgagcgag aaggcgtgga ccatcgccac ccgggcctgc
     4261 tccatcgacg tcgaggcgag cgcggggaag ccgatcacgt cgccggcggc gtacatcccc
     4321 ggcacgctgg tggcgtagtg ctcgtcgacc aggatcaggc cgcgcttgcc cagcgccacg
     4381 ccgagggcct cgaggcccag ccccgcgatg ttgctctgcc gcccggcggc aaagaacgcg
     4441 atgtcggccg caagcacctc gccgccggcc agcgtgagcg tcacgccgtc gtcccgtgcc
     4501 gccacctgcg ccacccggcc gttgaagacg gcgcggagcc cgaacccctc cagcctccgc
     4561 cgcagccggt cggcgatctc gccgtcgacg aaggggagga ggcggtcgcg cccctcgatc
     4621 aacgtcaccc ggacaccgag cgcggtgaag atggacgcgt actccgcgcc gatgaccccg
     4681 ccccccacca ccaccatcgt ccgcggcacc cggtccatcg acaggatcgt gtcggaatcg
     4741 tggaccaccc gcccgtcgaa cgggacttcg gggggccggt gcggcgacga cccggtggcg
     4801 atgagcaccc gctct
//
LOCUS       DASUAP010000305         5436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1290942, whole genome shotgun sequence.
ACCESSION   DASUAP010000305 DASUAP010000000
VERSION     DASUAP010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290942"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFU45_08210"
     CDS             complement(<1..319)
                     /locus_tag="VFU45_08210"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU5359089.1"
                     /translation="MLGAVVALLASYLVGAIPTSYLVGRVFRGIDLREHGSRNLGATN
                     LYRVLGWRFAVPVGLFDVAKGAAPVLVFGPLGPAATLFPLACGLTAVLGHVFSVFVGF
                     KGGK"
     gene            complement(319..1674)
                     /gene="der"
                     /locus_tag="VFU45_08215"
     CDS             complement(319..1674)
                     /gene="der"
                     /locus_tag="VFU45_08215"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU5359090.1"
                     /translation="MSQPTVAIVGRPNVGKSTLFNRLVGGRKAIVSEVPGTTRDRHFG
                     VVEWGGRSFWLVDTGGLVPDSDDAMDQAIRRQVEFAIAEADLILLVVDGKAGPHPADA
                     AIADRLRTVGRPVLLAVNKLDDHQRHGNDHLSFYELGMGDPLPVSAAVGSQTGDLLDR
                     VVEALPPQAEHEGEPMIDVAIVGRPNVGKSSLANRLLGDDRLVVSPVAGTTRDAIDTP
                     LRYHGETLNFIDTAGLRRRSKVEDDIEFYSTLRTDRAIERAQVCLLVVDAAAGLHNQD
                     LRIATEAWDRGAGLVVVVNKWDLIEEKDANTAHRGEQELAEKAPFLADVPFVYVSALT
                     GQRVRTLLDQVLVVAEARTRRIPTAEVNRVLGELVQRAAPPQKQGEEVKLLYASQIGT
                     EPPAFAIVSNRPDDVAESYTRYLLHGFRAAWGFAGCPVRLKYTARGARQHIKAQGDGR
                     R"
     gene            complement(1671..2954)
                     /locus_tag="VFU45_08220"
     CDS             complement(1671..2954)
                     /locus_tag="VFU45_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEU5359091.1"
                     /translation="MVKVTAVASGSLGEALGLRPGTELLAIDGRDLHDFLDWEFLAAA
                     EQFLLHVRHPDGEEIEFDVERPEDLPLGVAVEAVRIRRCANRCDFCFVEGLPEGLRDV
                     LYIRDDDYRLSFRYGNFATLTNLKPWDVARIKEYRLSPLYVSVHATDPAMRRRLLRNP
                     AAPEILPQLRDFQSAGIQFHTQIVVQPGINDGDVLVASLDDLWEFGPDILSVSVVPVG
                     LTEFSGHHKVRESTAEECRAMVATVDRFGARARAERGDTWCYGADELYLRAGVPLPPA
                     EVYGMFDQVENGVGSVRWLQREIAAGAAALGSAGWQGKRIAVCTGTSMGPLMPQVLAP
                     LSAATGADFELIVLVNPLFGSSVTTAGLLPGAAFQQALSGRRDLDLALIPGESLNDDG
                     LFMDDMPFDLLQAGVSCEVRPSKTFIDALEAPLPA"
     gene            complement(3135..3207)
                     /locus_tag="VFU45_08225"
     tRNA            complement(3135..3207)
                     /locus_tag="VFU45_08225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3172..3174),aa:Gly,seq:ccc)
     gene            complement(3261..4703)
                     /locus_tag="VFU45_08230"
     CDS             complement(3261..4703)
                     /locus_tag="VFU45_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5359092.1"
                     /translation="MTRILRTLAIGAFLAAPLAAQDPVIAMRMSSTKGDGSPIEPACK
                     RTLSPGHYLVSSGATYLQTAMKTSQPDNRSRALHDGQRVLLQAITQSGQGSNPAAWYY
                     LGRIDLMLGSVGGADSSLTKTTAMLPSCAAEVDLVRGEAVRLLAIPANDFQSAGKLDS
                     AMYLYRAAMKIDSTKPFVNYQMAEIFRGQKQDDSALVYYERAIAFAKDSTPVNKQIRG
                     NAGYTAGSIYYNAQRYADAVRAFRVAVDANPSDQDAIKNLSVSFRAAGMPDSAKALDD
                     QLLKQAQASGTVTPDQLFDLGVAQFNDKKYADAAATFRKVLAADPTRHDALENLASAY
                     SQLDSTTALIAVSEQLVGLEPLNYDAVKLLAIGYKKAEDKQKQKPKSFEAFNKLLGMT
                     VSLESVAFAPTDSGATLKLAAVGRAGADVRGNPLKPTAASIVVEFLDASGAVVASQPA
                     VAPALAPDARQDVTVSVTGKGIKGWRYRKP"
     gene            complement(4703..>5436)
                     /locus_tag="VFU45_08235"
     CDS             complement(4703..>5436)
                     /locus_tag="VFU45_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HEU5359093.1"
                     /translation="AHGVLAVPAPATLDLLTGLEIGPDGPVTGEATTPTGAVLLKALS
                     DGPPPARWRPVATGWGAGGRDPAGYPNALRLVIGERALEAGEAVVLSTDLDDLSPEYL
                     EPLREALMSAGALDVQIWPTQMKKGRIGFRVEIVTGPDAVSQVTDAVFRHSSTAGVRR
                     VAAERVTLNRRELVVPGTDHAPVRVKVLDGPGGLRIKPEYDDVHAAARQSGRPAHEVA
                     REVAERARAALNPAGDHGPITNQEQ"
BASE COUNT          864 a         1873 c         1857 g          842 t
ORIGIN      
        1 ccttcccgcc cttgaatccg acgaagaccg agaacacgtg ccccagcacg gccgtgaggc
       61 cacaggcgag cgggaatagc gtggcggccg ggccgagcgg accgaagacc aggaccggcg
      121 cggcgccctt ggcgacgtcg aagagcccca ccgggacggc gaagcgccat ccgaggaccc
      181 gatacaggtt ggtggcgccc aggttccggc tgccgtgctc gcggaggtcg atgccgcgga
      241 agacccgccc cacgaggtag ctggtgggaa tggcgccgac gagatacgaa gccaacagcg
      301 ccaccaccgc tccgagcatc agcgtctccc gtcgccctgc gctttgatgt gctgccgggc
      361 gccacgggcc gtgtacttga ggcgcaccgg gcacccggcg aacccccatg cggcccggaa
      421 cccgtgcagc aggtaccgtg tatacgactc cgccacgtcg tcagggcggt tcgaaacgat
      481 cgcgaaggcc ggcggctcgg tcccgatctg cgaggcgtag aggagcttga cctcctcacc
      541 ctgcttctgg ggtggcgccg cccgctggac cagctcaccc aggacgcggt tcacttccgc
      601 ggtcgggatc cgccgggtcc gcgcctccgc caccaccagc acctggtcga gcagtgtgcg
      661 gacccgctgg cccgtcagtg ccgagacgta gacgaagggc acgtcggcca ggaacggggc
      721 cttctccgcc aattcctgct cgccccggtg ggcggtattg gcgtcctttt cctcgatcag
      781 gtcccacttg ttgaccacga ccacgagccc ggccccgcgg tcccacgcct cggtcgcgat
      841 ccgcaggtcc tgattgtgga gccccgccgc ggcatccacc accaacaggc agacttgcgc
      901 ccgttcgatt gcgcggtcgg tgcggagcgt cgagtagaat tcgatatcat cttctacttt
      961 agatctgcgc cgtaaccccg cagtgtctat gaagttgagg gtctcgccgt ggtagcgcaa
     1021 gggggtgtcg atggcgtccc gggtcgtgcc cgcgaccggt gagaccacca gacggtcgtc
     1081 acccaggagc cggttggcca gggaggactt tcccacattg gggcggccga cgatcgcgac
     1141 atcgatcatc ggttccccct catgctctgc ctgcggcgga agggcttcga ccacccggtc
     1201 gaggaggtcg ccggtctggc tccccaccgc ggccgacacc gggagcgggt cgcccatgcc
     1261 gagctcgtag aagctcaggt ggtcgttccc gtggcgctgg tggtcgtcga gcttgttcac
     1321 ggccagcagg accgggcggc cgaccgtgcg gagccgatcg gcgatggcgg cgtcggcggg
     1381 atgcggcccc gccttcccgt ccaccaccag caggatcaga tccgcctccg caatggcgaa
     1441 ttccacctgc cggcggatgg cctgatccat cgcatcgtcg gaatcgggga ccaggccgcc
     1501 ggtgtcgacc agccagaacg aacggccgcc ccactcgacc acgccgaagt ggcggtcccg
     1561 ggtcgtgccc ggcacctcgg agacgatcgc cttccggccg ccgaccaggc ggttgaacag
     1621 ggtcgacttg ccgacgttgg gccggccgac gatggcgacg gtgggctggc tcatgcgggc
     1681 aggggcgcct ccagcgcgtc gatgaacgtc ttcgacgggc gcacctcgca ggacaccccc
     1741 gcctgcagca ggtcgaacgg catgtcgtcc atgaagagcc cgtcgtcgtt cagcgattcg
     1801 ccgggaatga gcgcgaggtc gaggtcgcgc cggcccgaga gcgcctgctg gaatgccgcg
     1861 cctggaagaa gcccggcggt cgtgacgctc gagccgaaga gcgggttaac caggacgatc
     1921 agctcgaagt cggcaccggt ggccgccgag agcggcgcga ggacctgagg catgagcggg
     1981 cccatcgaag tcccggtgca gaccgcgatg cgcttcccct gccacccggc gctcccaagc
     2041 gcagcggcgc ccgccgcgat ctcccgctgc agccaccgga ccgagcccac accgttctcc
     2101 acctggtcga acatcccgta gacctccgcc ggcgggagcg gtacgccggc gcgcaggtag
     2161 agctcgtcag cgccgtagca ccaggtgtca ccccgctccg cccgggcacg ggcaccgaag
     2221 cggtccacgg tggccaccat cgcgcggcac tcctctgccg tcgactcccg gaccttgtga
     2281 tggccgctga attcagtgag tcccactggc accaccgaca cgctcaggat gtccgggccg
     2341 aactcccaca ggtcgtcgag cgacgcgacg aggacatccc cgtcgttgat gccgggctgg
     2401 accacgatct gcgtatggaa ctggatgccg gccgactgaa agtcgcggag ctggggcagg
     2461 atctcgggcg ccgccggatt gcggagaagg cgtcggcgca tggcggggtc cgtcgcgtgc
     2521 accgagacgt agagcggcga gagccggtac tccttgatcc gcgcgacgtc ccacggcttg
     2581 aggttcgtga gcgtcgcgaa gttgccgtag cggaaggaga ggcggtagtc atcgtcacgg
     2641 atgtaaagga cgtcccgcaa gccctccggg agcccctcga cgaagcagaa gtcgcaacgg
     2701 ttggcgcacc ggcggatccg cacggcctcg accgcgactc cgagcggcag gtcctccggg
     2761 cgttcgacgt cgaactcgat ctcttccccg tcagggtgcc ggacgtgcag caggaactgc
     2821 tccgcggcgg cgaggaactc ccagtcgagg aaatcgtgga ggtcgcgccc gtcgatggcg
     2881 agcagctcgg tccctggacg gaggcccaac gcctcgccca gcgacccgga tgccaccgcc
     2941 gtgaccttga ccatcccgca ccgtcccaag ctgcaatgcc gacccggcgg tccgcgcgac
     3001 cgtccgagaa ccatgaaaga tggtaggata acggtccaag ttcaagtccc acagcgactt
     3061 ccgcgttcga tgctgcgggg tcagtggaaa caacgcgggg ccccgatgtg gggccccgcg
     3121 cgatgatcga acagagcggg agacggggtt cgaacccgcg accctcagct tgggaagctg
     3181 atgctctacc aactgagcta ctcccgcaac tcggggcgga acttgacggc ggttccaccc
     3241 caagtcaagc gccctgcggg tcagggcttc cgataccgcc agcccttgat gcccttcccc
     3301 gtcaccgaca cggtcacgtc ctgacgggcg tccggtgcca aggcaggggc caccgccggt
     3361 tggctcgcca ccacggcgcc cgacgcatcg aggaattcga ccacgatcga ggccgccgtg
     3421 ggcttcagcg gattgccgcg gacatcggcg ccggcgcggc cgaccgccgc cagcttgagg
     3481 gtggccccgg aatcggtggg cgcgaaggcc actgactcga ggctcacggt catcccgagg
     3541 agcttgttga acgcctcgaa gctcttcggc ttctgcttct gcttgtcttc ggccttctta
     3601 tagccgatgg ccagcagctt cacggcgtca tagttcaggg gctcgagccc caccagctgc
     3661 tccgacacgg cgatcagggc cgtggtggaa tcgagctgag aatacgcgct cgcgaggttc
     3721 tccagcgcat cgtgccgggt cgggtccgcc gccagcacct tgcggaaggt ggcggccgcg
     3781 tcggcgtact tcttgtcgtt gaactgcgcc acaccgaggt cgaagagctg atccggcgtc
     3841 acggtgccgg aggcctgggc ctgcttgagg agctggtcgt cgagcgcctt ggcgctatcg
     3901 ggcatcccgg cggcacggaa ggagactgac aggttcttga tggcgtcctg gtcgctcgga
     3961 ttggcgtcca ccgccacgcg gaatgcgcgc acggcatcgg cgtagcgctg ggcgttgtag
     4021 tagatcgagc cggccgtgta cccggcgttc ccgcggatct gcttgttgac cggcgtcgag
     4081 tccttggcga aggcgatggc ccgctcgtag tagacgagcg cgctgtcgtc ctgcttctgc
     4141 ccccggaaga tctccgccat ctggtagttc acgaacggct tggtcgagtc gatcttcatg
     4201 gccgcccgat agaggtacat cgcggaatcg agcttcccag cgctctggaa gtcgttggcc
     4261 gggatcgcca ggaggcggac cgcctcgccg cggaccaggt cgacttccgc ggcgcagctc
     4321 ggcagcatgg cggtcgtctt ggtcagggaa gaatcggcgc cgccgacgct cccgagcatc
     4381 aggtcgatgc gaccgaggta gtaccaggcc gcggggttcg atccctggcc gctctgagtg
     4441 atcgcctgca ggagcacacg ctgcccgtca tgcagggccc ggcttcggtt gtcgggctgg
     4501 gaggtcttca tcgcggtctg gaggtacgtg gcgccgctgc tcacgagata gtggccaggg
     4561 ctcaacgtgc gcttgcacgc cggttcgatc ggcgagccat cgcccttggt gcttgacatc
     4621 cgcatggcaa tcacgggatc ctgggcggcg agcggcgcag caagaaaggc accgatggcc
     4681 aacgtgcgaa ggattcgggt catcactgct cctggttcgt gatcgggccg tggtcgccgg
     4741 ccgggttgag cgcggcgcgg gcccgttcgg ccacctcgcg cgccacttcg tgcgccggac
     4801 gccccgattg gcgcgccgcc gcatgaacat cgtcgtattc tggcttgatc ctcagcccac
     4861 cggggccgtc cagcaccttg accctgactg gagcatggtc cgtgccaggc accaccaact
     4921 cccgcctgtt cagggtgacg cgctcggcgg cgacccgacg caccccggca gtggaactgt
     4981 gccggaagac cgcatcggtg acttgggata ccgcgtcggg accggtcacg atctcgaccc
     5041 ggaatccgat ccgccccttc ttcatctggg tcggccagat ctggacatcc agggcgccgg
     5101 ccgacatcag cgcttcccgc agcggctcga gatactccgg actcaggtcg tcgaggtcgg
     5161 tcgagagcac caccgcttcg cccgcctcga gtgcccgctc accgatgacc aggcggagcg
     5221 cattgggata gccagcaggg tcgcgcccac cggctcccca ccccgtcgcc accggccgcc
     5281 accgcgccgg gggtgggcca tcgctcaggg ccttgagcag gaccgcgccg gtgggggtgg
     5341 tggcctcgcc ggtcacaggg ccatcggggc cgatctcgag cccggtcagc aggtcgagcg
     5401 tcgccggcgc cgggacggcg agcacgccgt gcgcag
//
LOCUS       DASUAP010000306         4456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_677918, whole genome shotgun sequence.
ACCESSION   DASUAP010000306 DASUAP010000000
VERSION     DASUAP010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677918"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /gene="hpt"
                     /locus_tag="VFU45_08240"
     CDS             <1..501
                     /gene="hpt"
                     /locus_tag="VFU45_08240"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU5359094.1"
                     /translation="IVYDAATIAARVKELGAEITAAYPEGELLVLGLLKGSFIFLGDL
                     VRHIERKLQVDFLVASSYGAGKVSSGNVRLLYDPETKLEGKHILVVEDIIDSGKTLNR
                     LLALLAERRPKSLAICALLDKHVAPEPPKHVRFTGFDAPPAFLVGYGLDHAEEYRHLP
                     FIADLE"
     gene            511..2511
                     /gene="ftsH"
                     /locus_tag="VFU45_08245"
     CDS             511..2511
                     /gene="ftsH"
                     /locus_tag="VFU45_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5359095.1"
                     /translation="MPDRLPPPRQQGPDWASVSKSAALWAMIILAALFLFNVMGRQRG
                     GVQEFTYTGFRAQLDAGNVATATYVTGATDQRLQGDFKAAVTTPDGRSAKGYTVLLPV
                     GNSEQLLQALDAAKVQVNAKEDSGTFATILLGLLPYVLIFALAIFFLRQMQAGGNRAF
                     SFGKSKAKLLSGDTPKITFADVAGADEAKVELQEIIEFLKDPQKFTRLGGRLPKGALL
                     VGAPGTGKTLLAKAVAGEAGRPFFSMSGSDFVEMFVGVGASRVRDLFEQGKAHAPCII
                     FIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNDGVILIAATNRPDVLDPA
                     LLRPGRFDRQIVVDVPDVKGREGILRVHTRKIPLGANVRLDVVARGTPGMAGADLANL
                     VNEAAVLAARRNKSLVDMADFEDAKDKVMLGLERRSLVLTEDERRLTAYHEAGHTIVL
                     VKTPGADPIHKVTIVPRGRALGLTMWLPETDRHGMSKELLLGQLAIAFGGRVAEELVF
                     GPEKVTTGAGGDIQQATKIARQMVTRYGMSDKIGMMATSEAETEIFLGREFGQRREVS
                     ERTAELVDEEVKRLIDEAYRRARNILESERALLDRMAATLLDRETIDRDDVDLLVQGK
                     PLPPRAEPPAPTAPAPALTAAPKPAPGLGPILGAPPAKPAGA"
     gene            2508..3677
                     /gene="folP"
                     /locus_tag="VFU45_08250"
     CDS             2508..3677
                     /gene="folP"
                     /locus_tag="VFU45_08250"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU5359096.1"
                     /translation="MKAGALPVHGPRAVVAALAAHGWEAHRAEAAAGGLGPVALHLRD
                     VPAPAIEALVSWNARAALDLLTGPDWVIIAGTRSRVSALARPWTVPPALSELAVAVGM
                     ALPAEPVPEWVTARGRIALDRGVLVGIVNVTPDSFSDGGAHVSVDAAVAHAARLLDDG
                     ATMLDVGGESTRPGAAPVPVEEELRRAVPVVEALASRFPETPISIDTVKAPVARAALE
                     AGAWAVNDVSALRLDPDIAALVAAAGAGIVLMHSRGAFSEMATFDHTEYPAGVIDGVA
                     VELGAAVARATAAGIGAERIVLDPGFGFSKTAEQNLALLDGLAGLAGLGHPLMVGPSR
                     KRFLGHATGRDLADRDRATAAACLLAWERGARLFRVHEPAAVQDALALARSVGDA"
     gene            3677..>4456
                     /gene="cdaA"
                     /locus_tag="VFU45_08255"
     CDS             3677..>4456
                     /gene="cdaA"
                     /locus_tag="VFU45_08255"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEU5359097.1"
                     /translation="MPVSQIHFLAPTWRDALEVALVAFIIYRLLRVLAGTRALQIVFG
                     VLVLLVAYVAAFLLKLTMITYLLGVLFTYGAFAVIVVFQPELRQALARLGQTRFLRAF
                     TASNVDRVTEAVAEAVERLARNATGAIIVLEKDAALDEYVRTGTGIDAAVSADLLATI
                     FTPYSPLHDGAVLIRGDRIIGAGCILPLTQFPVADRALGTRHRAALGLSEETDALVLV
                     VSEENASVSLACEGRLARSLTFEQVRAVLDGPWPPDLRQFDP"
BASE COUNT          619 a         1611 c         1579 g          647 t
ORIGIN      
        1 atcgtgtacg atgccgccac catcgccgcc cgggtgaagg aactcggcgc cgagatcacc
       61 gccgcgtacc cggaggggga gctgctggtg ctgggcctcc tcaaggggag cttcatcttc
      121 ctgggagact tggtccgcca catcgagcgg aagctgcagg tggacttcct ggtggcgtcg
      181 agctacgggg cggggaaggt ctccagcggc aatgtccggc tcctgtacga tccggagacc
      241 aagctcgagg ggaaacacat cctcgtcgtc gaggatatta ttgatagcgg caagacgctc
      301 aaccggcttc tggcgcttct ggccgaacgc cgaccgaaga gcctggccat ctgcgccttg
      361 ctggacaagc atgtggcgcc cgagccgccg aagcacgtgc ggttcacggg gttcgacgcg
      421 ccgccggcgt tcctggtcgg gtatgggctc gaccacgccg aggagtaccg gcatttgccg
      481 ttcatcgcag acctggagta ggaccgaacg atgcctgacc gacttccgcc gccgcgccag
      541 caggggcccg actgggcgag cgtgagcaag agcgctgccc tctgggccat gatcatcctt
      601 gcggcgctgt tcctgttcaa cgtgatgggg cgccagcgcg ggggcgtgca ggagttcacg
      661 tacaccggct tccgcgccca gctcgacgcc ggcaacgtgg ccaccgccac gtatgtcacc
      721 ggcgccaccg accagcgcct gcaaggtgac ttcaaggccg cggtgaccac ccccgacggc
      781 cggagcgcga agggctacac agtcctcctc ccggtgggga acagcgagca gctgctgcag
      841 gccctcgacg ccgccaaggt gcaggtgaac gccaaggagg acagcgggac gttcgccacg
      901 atcctcctcg gcctcctgcc gtacgtgctc atctttgcgc tcgcgatctt cttcctccgc
      961 cagatgcagg cgggcgggaa ccgggcgttc agcttcggca agagcaaggc caagctgctc
     1021 tcgggcgaca cgcccaagat caccttcgcc gacgtggccg gcgccgacga ggccaaggtc
     1081 gagctgcagg agatcatcga gttcctcaag gacccgcaga agttcacccg cctcggcggc
     1141 cggctcccca agggggcgct gctggtgggc gcgccgggca ccggcaagac gctcctcgcc
     1201 aaggccgtcg ccggcgaggc ggggcgcccg ttcttctcga tgtccgggtc cgacttcgtc
     1261 gagatgttcg tcggcgtcgg cgcctcgcgg gtgcgcgacc tcttcgagca ggggaaggcc
     1321 cacgccccct gcatcatctt catcgacgag atcgacgccg tggggcgcca ccgcggcgcc
     1381 ggcctgggcg gcgggcacga cgagcgcgag cagacgctca accagctcct ggtcgagatg
     1441 gacggcttcg agtcgaatga cggggtcatc ctcatcgccg ccaccaaccg ccccgacgtc
     1501 ctcgacccgg cgctcctccg cccggggcgt ttcgaccgcc agatcgtggt ggacgtgccc
     1561 gacgtgaagg gccgcgaggg gatcctccgc gtccacaccc gcaagatccc cctcggcgcc
     1621 aacgtccggc tcgacgtcgt ggcccggggc acgccgggga tggcgggcgc cgacctggcc
     1681 aacctcgtca acgaggcggc cgtcctcgct gcgcgccgga acaagtcgct ggtcgacatg
     1741 gcggacttcg aggacgccaa ggacaaggtg atgctcggcc tggagcgccg ctcgctggtg
     1801 ctcaccgagg acgagcgccg cctcaccgca taccacgagg ccggccacac gatcgtgctg
     1861 gtcaagacgc cgggcgccga cccgatccac aaggtcacca tcgtcccccg cgggagggcg
     1921 ctcgggctca cgatgtggct ccccgagacc gaccgccacg gcatgtcgaa ggaactcctc
     1981 ctgggccagc tcgcgatcgc cttcggcggg cgggtggccg aggagctcgt cttcgggccg
     2041 gagaaggtga ccaccggggc gggcggcgac atccagcagg ccaccaagat cgcgcgccag
     2101 atggtgacgc ggtacgggat gagcgacaag atcgggatga tggccaccag cgaggccgag
     2161 acggagatct tcctcggccg cgagttcggc cagcgccgcg aggtgtccga gcgcaccgcc
     2221 gagctggtgg acgaagaggt caagcggctc atcgacgaag cgtaccggcg cgcgcggaac
     2281 atcctcgagt cggagcgcgc gctcctcgac cggatggccg ccaccctcct cgaccgcgag
     2341 accatcgatc gggacgatgt cgacctgctg gtgcagggga agccgctgcc gccccgggcg
     2401 gagccgccgg cgcccaccgc gccggcgccg gccctgaccg cggcgcccaa gccggcgcca
     2461 ggcctcggtc cgatcctcgg cgcgccgccc gccaagcccg ccggggcgtg aaggccggag
     2521 cgctgccggt ccacggaccg cgcgccgtcg tcgcggcgct ggcagcgcac ggatgggagg
     2581 cccaccgggc cgaggccgcc gccggcggtc tcggtccggt cgcgttgcac ctccgtgacg
     2641 tcccggcgcc cgccatcgag gcgctcgtct cgtggaatgc gcgggccgcc ctcgacctcc
     2701 tcaccggccc ggactgggtc atcatcgcag ggacccggag ccgcgtcagc gccctcgcgc
     2761 gcccgtggac cgtgccgccg gcgctctcgg aacttgccgt ggcggtcggg atggcgctcc
     2821 ccgccgaacc ggtgccggaa tgggtcaccg cccgcggccg cattgcgctc gaccgcggcg
     2881 tcctcgtcgg gatcgtgaac gtcaccccgg acagcttcag cgatggcggt gcgcacgtgt
     2941 cggtcgatgc cgccgtggcc cacgccgcgc gcctcctcga cgatggcgcg acgatgcttg
     3001 acgtgggcgg ggagtcgacc cgtcccggcg cggcgccggt gccggtcgaa gaggaactcc
     3061 ggcgcgccgt gcccgtggtc gaggcgctgg ccagccggtt cccggagacg cccatctcca
     3121 tcgacacggt caaggcgccg gtggcgcgcg cggccctcga ggcgggcgcg tgggcggtca
     3181 atgacgtgag cgccctccgc ctcgatccgg acatcgcggc gctggtcgcc gcggccggcg
     3241 cggggatcgt cctgatgcac tcgcggggtg ccttctcgga gatggccacc ttcgaccaca
     3301 cggagtatcc ggcgggggtg atcgatggcg tcgccgtcga gctcggcgcg gcggtggcgc
     3361 gggcgacggc cgccgggatc ggtgccgagc ggatcgtgct ggatcccggg ttcgggttct
     3421 ccaagaccgc ggagcagaac ctggcgctgc tcgacgggct ggccgggctc gcagggctcg
     3481 gtcacccgct gatggtcggt ccctcccgga agcggttcct gggtcatgcc accggccgcg
     3541 acctggccga ccgcgatcgc gccaccgcgg ccgcctgcct cctggcctgg gagcgcggcg
     3601 cgcggctctt ccgggtgcac gagcccgccg cggtgcagga cgcgctggcg ctggcccggt
     3661 ccgtgggaga cgcctgatgc cggtgtccca gatccacttc ctggcgccga cctggcgcga
     3721 tgcactggag gtggcgctcg tcgcgttcat catctaccgc ctgctccggg tcctggccgg
     3781 cacccgtgcg ctccagatcg tcttcggggt gctggtgctg ctcgttgcct acgtcgcggc
     3841 atttctcttg aagctcacga tgatcaccta cctcctgggt gtgctcttca cctacggggc
     3901 gttcgcggtg atcgtcgtct tccagcccga gctgcgccag gcactcgccc ggctggggca
     3961 gacccgcttc ctccgggcgt tcacggcaag caacgtcgac cgcgtcaccg aggcggtggc
     4021 ggaggcggtg gagcggctgg cccggaacgc caccggcgcc atcatcgtgc tggaaaagga
     4081 cgcggcgctc gacgagtacg tccggaccgg cacggggatc gacgccgccg tttccgccga
     4141 cctgctggcc acgatcttca cgccgtattc gcccctccac gacggcgcgg tgctcatccg
     4201 cggcgatcgc atcatcggcg ctgggtgcat cctgccgctc acccagttcc ccgtcgccga
     4261 ccgcgcgctc ggtacccgcc accgcgctgc cctgggcctc tcggaggaga ccgacgccct
     4321 ggtgctggtc gtgtcggagg agaatgcgtc ggtgtcgctc gcctgcgagg gccggctggc
     4381 gcggtcgctc accttcgagc aggtccgcgc ggtgctcgac ggcccgtggc cccccgacct
     4441 tcggcagttc gacccg
//
LOCUS       DASUAP010000307         6403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1182641, whole genome shotgun sequence.
ACCESSION   DASUAP010000307 DASUAP010000000
VERSION     DASUAP010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182641"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFU45_08260"
     CDS             <1..370
                     /locus_tag="VFU45_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5359098.1"
                     /translation="IEALAAGAAGFMLKQASSRELAQAIASIVSGGTYVDPMIAQKID
                     AGETEGSATRDGELLLTYRQREVLRLIALGRSNVEIAIELGITVKAVEFHRSRIWRAL
                     GVSTSAAMISVAVARGLARP"
     gene            416..694
                     /locus_tag="VFU45_08265"
     CDS             416..694
                     /locus_tag="VFU45_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HEU5359099.1"
                     /translation="MLLMDRENLLARGWSHVVSDTDDLAELEELRVRVGAPPRALQFG
                     DGHRPHLDLKLEPRERALADPGVRVFDRTRDLVRHLHSLTGSPRDRVR"
     gene            691..1098
                     /locus_tag="VFU45_08270"
     CDS             691..1098
                     /locus_tag="VFU45_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5359100.1"
                     /translation="MNRFRILIADDHHLFCDGLRKILEPEHHVVGTVHDGLDVVPAIL
                     SLEPDLVLLDLSLPGRNGIEIARQARDLGLAARILVLTMHNDTVFAVEALRAGAAGFM
                     LKEASGEELLHAVDEVMDGRTYLDAELRRLAGM"
     gene            complement(1267..2583)
                     /locus_tag="VFU45_08275"
     CDS             complement(1267..2583)
                     /locus_tag="VFU45_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5359101.1"
                     /translation="MIPLPSRRWFRVAAALAVAAPLALLWPEASAFLVVADLCWVAAF
                     WLDWYLSPAESDISVERDAPAAFSVGRAFPVRYRWTRRGSRGFVAQVRESYPAPLMQG
                     PSPMRRLVIPAGGATESITIRPVRRGRGTAGTLAVRLGGPMGLAWSQGKIERPWTATV
                     FPDLTDVAVRALPTQSARRREAGLRNVRRLGEGRVFETLRDWVPGDDTRTIDWKATAR
                     RGKTMARQYEDERRQQVLIVIDAGRMLTAEVHGRPRLESAIDAAVQLAWTAIDHDDNV
                     GLLVFADGIQHWLPPRRGKRALQGILEALATVEGKLVEPNYPAAFAHLALRNRKRALT
                     VLFTDLIDRTASDALVAQAGTLRPRHLPLAVTFRNPELEALALQPPGKLDEAYERAAA
                     EELLVARAEALAAVRGRGVLVLDVLPEGAAHAVVERYRQLKRRGML"
     gene            complement(2580..4514)
                     /locus_tag="VFU45_08280"
     CDS             complement(2580..4514)
                     /locus_tag="VFU45_08280"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5359102.1"
                     /translation="MRTPSLFLLLAAASAPAAAQQPARAVRRTIPITRAFEAGLASGA
                     RDSLGTPGSRYWQLRTDYVIDAKLDPGTGVVTGRETIAIDNPSDSALGVVYLRLLQNL
                     FAPNVERAESEPSLTDGMKVTKLVANGTSVNVDSAAEDWSHRTVVRVRLATPIPAHGT
                     GTIAAEWSFTVPMVPLDERGDRMGSWEHRLYQVAQWYPQLAMYDDLRGWNTDPYLGAA
                     EFFNNFGSFDVRLDLPAGWLVGATGVLQNADSVLTPMVRARLAHVTESDSQVSIVGVD
                     ERGAGKATMAGDRLVWHFRADSVADFAWAASNEFVWDATRATIPGKGVIPVNILYWPE
                     HADYRQTGVYARHALEYYSKLWMPYAFPQLTQVDGPELGMEYPMFIMSGSGRGVTDHE
                     IGHEWWPMMVGVNETWYGWMDEGFNQYMNRLSSFDFRGLPANNDSLGQAYGKVSGRES
                     EPPMMWDGNYAGPMYGFTTYGKAPLMLSALGGVVGDSAVWRAMRTYANAWRFKHPSPW
                     DFMFAMDHALKQDLGWFWYYWLFTTESVDGSIARVEARGVRTLVTVHQAGEMPSPVVL
                     RVEFAPNGPAIRPMTNAIVTGSTATVTWPVDVWFGGARDFTATLDFGARKIERITLDP
                     GMRFPDRDPADNTWPRAPTK"
     gene            complement(4555..5535)
                     /locus_tag="VFU45_08285"
     CDS             complement(4555..5535)
                     /locus_tag="VFU45_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5359103.1"
                     /translation="MAGIEALTPDAARRLSEVARRIEREVASVVLGQPDAGRDVLVAL
                     LARGHVLLEGVPGTAKTLLVRALARAVGVGFRRIQFTPDLMPSDITGINLLTGQGQFT
                     FRAGPIFADFVLADEINRAPAKTQAALLEAMQERSVTVDGETHPLPAPFTVFATQNPV
                     EFEGTYPLPEAELDRFVVKVLVGYPDAATEQGILTRVLGGFESDDPSSYGTSMAVDAA
                     GLAELRLAARGVRAEPAITQYVTALVRATREAPSITMGASPRATVWLLKASQAAALLD
                     GREYVTPDDVKAMAPAVLRHRIVVAPELELEGVSPDRALEGILQAVEVPR"
     gene            complement(5514..6302)
                     /locus_tag="VFU45_08290"
     CDS             complement(5514..6302)
                     /locus_tag="VFU45_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359104.1"
                     /translation="MAGIGPRPAYVSAVLGRTGVAAVADTMDAGAGVIARCRVPAVTE
                     RDTVLLTTGNRLVAIRLRLSGGHAVTLVSDGRLFGNRSLKDTDAAAAVVPLLAGRARA
                     VYFDEYHQGYASNGSLLRETLAWSRRSPWGWGAWQLAVVGLLALLAAAVRFGPVVPGI
                     ERRRRSPMEHVRALATALAAARGHDVAVRLMVRGLRRRLSRDNRPQPEPVESWLAQLA
                     DRVRSTRARAAVNDLQSLIHPPQPADAVLRAAYAVEDVWQELRP"
BASE COUNT          967 a         2328 c         2238 g          870 t
ORIGIN      
        1 gatcgaggcg ctggcggcgg gcgccgcggg gttcatgctc aaacaggcgt cgagccggga
       61 gctcgcccag gccatcgcga gcatcgtgtc gggcggcacg tacgtggacc cgatgatcgc
      121 ccagaagatc gacgccggcg agaccgaagg gagcgccacc cgcgacggcg aactcctcct
      181 cacctaccgc cagcgcgagg tgctccgcct gatcgccctc ggccgctcca acgtcgagat
      241 cgccatcgag ctcggcatca ccgtcaaggc cgtcgaattc caccggagcc ggatctggcg
      301 ggcactcggc gtctccacct cggcggcgat gatcagcgtg gcggtcgcgc gcggcctggc
      361 gcggccctga ctatattatc ccaccccctg tagcccaccc cctgataaca cgccgatgct
      421 gctgatggac cgagagaatc tcctggcccg ggggtggtcg cacgtcgtga gcgacaccga
      481 cgacctggcc gaactggagg agctccgggt gcgggtcggg gcgccgcccc gcgccctgca
      541 gttcggcgac ggccaccgcc cccacctcga cctcaagctc gagccgcggg agcgggccct
      601 cgccgacccg ggcgtgcgcg tcttcgaccg gacccgcgac ctcgtccgtc acctgcattc
      661 gctcaccgga agcccgagag accgcgtgcg atgaatcgct ttcgcatcct gatcgccgac
      721 gaccatcacc tcttctgcga cgggctccgc aagatcctcg agccggaaca ccacgtcgtg
      781 ggcaccgtgc acgacgggct cgacgtggtg cccgcgatcc tgtccctcga acccgacctc
      841 gtgctcctcg acctctcgct ccccggccgc aacgggatcg agatcgcgcg gcaggcgcgc
      901 gacctggggc tcgcggccag gatcctcgtc ctcacgatgc acaacgacac ggtcttcgcc
      961 gtcgaggcgc tccgggccgg cgcggccggg ttcatgctca aggaggcgtc gggcgaggaa
     1021 ttgctgcacg cggtcgacga ggtgatggac ggccgaacgt acctggatgc ggaactgcgc
     1081 cggctggcgg ggatgtgaga agtaagaagt gagaagtgag aagtaagaag ggagagggaa
     1141 aaggaaactg ggaacaggga agaggtgaga ggtaagaggt gagcgggccg ccactcgaga
     1201 cccgtggcac gcgcccgttc accgttcacc gttcactgtt caccgcttac ccctcaccgc
     1261 tcaccgtcag agcatccccc gccgcttgag ctggcggtac cgctccacca ccgcgtgggc
     1321 agcgccttcc ggcagcacat cgagcaccag cacaccgcgt ccacgcaccg ccgccagcgc
     1381 ctcggcccgc gccaccagca gctcctccgc cgcggcgcgc tcatacgcct cgtccagctt
     1441 cccggggggc tggagcgcca gcgcctccaa ctcggggttc cggaacgtca ccgccagcgg
     1501 caggtgccgg ggacggagcg tgcccgcttg cgccaccagg gcgtcgctcg cggtgcggtc
     1561 gatgaggtcc gtgaagagca ccgtgagcgc gcgcttccgg ttccggagcg ccaggtgcgc
     1621 gaacgccgcg gggtaattcg gctcgaccag cttcccctcg acggtcgcga gcgcctccag
     1681 gatgccctgc agcgcccgct tcccccgccg gggcgggagc cagtgctgga tcccgtccgc
     1741 aaagaccagg agcccgacat tgtcgtcgtg gtcgatcgcg gtccacgcaa gctgcactgc
     1801 ggcgtcgatg gccgactcga gccgcggccg cccatgcacc tcggcggtga gcatccggcc
     1861 ggcatcgatc acgatcagca cctgctggcg ccgctcgtcc tcgtactgcc gcgccatcgt
     1921 cttcccgcgg cgcgcggtcg ccttccagtc gatggtccgg gtgtcgtccc cgggcaccca
     1981 gtcccgcagg gtctcgaaga ccctcccttc cccgagccgg cgcacgttgc ggagccccgc
     2041 ctcccggcgc cgggcggact gcgtcggcaa tgcccggacg gcgacgtccg tgaggtcggg
     2101 gaacacggtg gccgtccaag gacgctcgat cttcccctgc gaccaggcga gccccatcgg
     2161 cccaccgagc cggacggcca gcgtgccggc ggtaccacgc ccgcgccgga cgggacggat
     2221 ggtgatggac tcggtggcgc cccctgccgg gatgacgagg cgccgcatcg ggctcggccc
     2281 ctgcatcagc ggcgccggat agctctcccg cacctgtgcg acaaagcccc gcgatccacg
     2341 ccgggtccag cggtaccgca ccgggaacgc gcggccgacg ctgaacgcgg ccggtgcgtc
     2401 ccgctccacc gagatgtcgg actccgcggg cgagagatac cagtcgagcc agaacgcggc
     2461 gacccagcag aggtccgcca cgaccaggaa tgccgatgct tccggccaga gcagggcgag
     2521 cggcgccgcc acggccagcg cggccgcgac ccggaaccac cgccggctcg ggagcgggat
     2581 cacttcgtcg gcgcccgggg ccaggtgttg tcggccggat cgcgatcagg gaagcgcatt
     2641 cccggatcga gggtgatccg ctcgatcttc cgcgcgccga agtcgagggt ggccgtgaag
     2701 tcgcgcgcgc cgccgaacca gacatccacc ggccacgtga cggtggcggt gctccccgtg
     2761 acgatggcat tcgtcatcgg ccggatggcg gggccgttcg gggcgaactc cacccgcagc
     2821 acgaccggcg agggcatttc gcccgcctgg tgcaccgtga cgagcgtgcg aacgcccctg
     2881 gcctccaccc gcgcgatcga cccgtccacc gactccgtgg tgaagagcca gtagtaccag
     2941 aaccacccga ggtcctgctt gagcgcgtgg tccatcgcga acatgaagtc ccacggcgac
     3001 gggtgcttga agcgccaggc gttggcgtac gtgcgcatcg cacgccagac ggcggaatcg
     3061 cccaccacgc cccccagcgc cgagagcatc agaggagcct tgccgtaggt cgtgaacccg
     3121 tacatcggcc cggcgtagtt gccgtcccac atcatcggcg gctcgctctc ccgccccgag
     3181 accttcccgt aggcctggcc gagggagtcg ttgttggccg gcaggccccg gaagtcgaag
     3241 gacgacagcc ggttcatgta ctgattgaat ccctcgtcca tccagccgta ccaggtctcg
     3301 ttgacgccga ccatcatcgg ccaccattca tggccgatct cgtgatcggt gacgccccgt
     3361 ccgctgccgc tcatgatgaa catcgggtat tccatcccga gctcggggcc gtccacctgg
     3421 gtgagctggg gaaaggcgta cggcatccag agcttcgagt agtactcgag cgcatgccgc
     3481 gcgtagacgc cggtctgccg gtagtcagcg tgctccggcc agtagaggat attgaccgga
     3541 atgacgccct tcccggggat ggtggcgcgg gtggcgtccc agacgaactc gttcgacgcg
     3601 gcccaggcga agtcggccac gctgtcggcc cggaagtgcc acaccaggcg gtctcccgcc
     3661 atggtggcct tccccgcccc gcgctcatcg acgccgacga tgctcacctg gctgtcactc
     3721 tccgtgacgt gggccaggcg cgcgcgcacc atcggcgtca gcacggagtc ggcgttctgc
     3781 agcaccccgg tggcgccgac cagccatccg gccgggaggt cgagccggac gtcgaacgac
     3841 ccgaagttgt tgaagaactc cgccgcgccg aggtaggggt cggtgttcca gccgcggagg
     3901 tcgtcgtaca tcgcgagctg cgggtaccat tgcgccacct ggtagagccg gtgctcccag
     3961 ctccccatcc ggtcgccgcg ctcatcgagc ggcaccatcg gcaccgtgaa cgaccactcg
     4021 gccgcgatgg tgccggtgcc gtgcgcgggg atgggcgtgg ccagccgtac ccggaccacc
     4081 gtccggtggc tccagtcctc cgccgcgctg tcgacgttca cggacgtgcc gttggcgacg
     4141 agtttcgtga ccttcatccc gtccgtgagg ctcggctccg attccgcccg ctcgacgttc
     4201 ggggcaaaga ggttctgcag gagccggagg tacacgacgc cgagggcaga gtcgctggga
     4261 ttgtcgatgg cgatcgtctc gcggccggtg accactcccg tgccggggtc gagcttcgcg
     4321 tcgatcacgt agtcggtccg gagctgccag tatctcgacc ccggcgtgcc gagggagtcc
     4381 cgcgcgccgg aggcgagccc cgcctcgaag gcgcgggtga tcgggatggt gcgccggacg
     4441 gcgcgggccg gctgctgcgc ggccgccgga gcggacgcgg cggccagcag gaggaagagc
     4501 gaaggtgtgc gcatacggac tcccatcgat ggtgaacggc tgcgacgtcc cggttcagcg
     4561 cgggacttcg accgcttgga ggatcccttc cagcgcccga tcgggggaga ccccctcgag
     4621 ctcgagttcc ggcgccacca cgatccggtg gcgaagcacc gcgggcgcca tcgccttgac
     4681 gtcgtccggc gtgacgtact cccggccatc gaggagcgcc gccgcctggc tcgccttgag
     4741 caaccagacg gtggcgcggg gcgaggcgcc catcgtgatc gacggcgcct cccgcgtggc
     4801 ccggacgagc gccgtgacgt actgcgtgat cgcgggctcg gcccgcacgc cacgcgcggc
     4861 caggcggagt tcggcgagcc ccgcggcgtc caccgccatc gacgtgccgt agctcgacgg
     4921 gtcgtccgac tcgaagccac cgagcacgcg ggtcaggatc ccctgctccg tggccgcgtc
     4981 gggatagccc acgagcacct tgaccacgaa gcggtcgagt tcggcctcag gcaggggata
     5041 ggtcccctcg aattccaccg ggttctgggt ggcaaagacc gtgaacggcg ccgggagcgg
     5101 atgggtctcg ccgtcgaccg tgacgctccg ttcctgcatc gcctcgagca gcgcggcctg
     5161 ggtcttggcg ggcgcccggt tgatctcgtc ggcgaggacg aagtcggcga agatgggtcc
     5221 ggcccggaag gtgaattggc cctggccggt aaggaggttg atgccggtga tgtccgaggg
     5281 catcaggtcc ggggtgaact ggatccggcg gaagccgacc ccgaccgccc gggccagcgc
     5341 gcgcaccaga agggtcttgg cggtgccggg caccccttcg agcagcacgt gcccacgggc
     5401 gaggagcgcg acgagcacgt cgcgcccggc gtccggctgc ccgaggacga ccgacgcgac
     5461 ctcgcgttcg atccggcgcg ccacttccga caggcggcgg gcggcgtccg gggtcagggc
     5521 ctcaattcct gccacacatc ctccacggcg taggccgccc gcagcacggc atcggccggc
     5581 tggggcgggt ggatgaggga ctgcagatcg ttgacggcgg cgcgggcgcg ggtggagcgc
     5641 acgcgatcgg ccagttgcgc gagccacgac tcgaccggct cgggctgggg tcggttgtcg
     5701 cgggagaggc ggcgccggag cccccgcacc atcaggcgca ccgcaacgtc gtggccccgc
     5761 gcggcagcga gcgcggtggc aagcgcccgc acgtgctcca tcggcgagcg gcgccggcgc
     5821 tcgatgcccg gcaccaccgg gccgaagcgg acggccgcgg cgaggagcgc caggagcccc
     5881 accacggcga gctgccaggc gccccatccc cacggcgagc gccggctcca cgccagcgtc
     5941 tcgcggagga ggctcccgtt cgacgcgtag ccctgatggt actcgtcgaa gtagaccgcg
     6001 cgggcccgcc ccgcgagcag cggcaccacg gccgccgcgg catcggtgtc cttgaggctc
     6061 cggttgccga agagccgccc gtccgacacg agggtcacgg cgtgtccgcc cgacagccga
     6121 agccggatcg cgaccaggcg gttcccggtg gtaaggagca cggtgtcccg ctccgtcacc
     6181 gccggcaccc ggcaccgcgc gatgaccccg gcgccggcat ccatcgtgtc cgccaccgcg
     6241 gccaccccgg tccggcccag caccgccgag acataagcgg gccgcgggcc gatccccgcc
     6301 accgtgatcg agtccgcgcg cggcagcgag cgcggtggca agcgcccgca cgtgctccat
     6361 cggcgagcgg cgccggcgct cgatgcccgg caccaccggg ccg
//
LOCUS       DASUAP010000308         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_432586, whole genome shotgun sequence.
ACCESSION   DASUAP010000308 DASUAP010000000
VERSION     DASUAP010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432586"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFU45_08295"
     CDS             complement(<1..259)
                     /locus_tag="VFU45_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359105.1"
                     /translation="MIHSSTLFDQSPAAEAAIEPHAVFLARAASAREAGDDRGAELAL
                     GAYLTLRAVDRAATLGGEAAADVREGFRFQAQAAFKFVDRLP"
     gene            409..843
                     /locus_tag="VFU45_08300"
     CDS             409..843
                     /locus_tag="VFU45_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5359106.1"
                     /translation="MRQGFTLPEVAITLALVGILTGFAAPSLMAAADRGAIRMGLAQL
                     RGAHEEARLTAVLDDRVSVLTIDSLAITLRTVLGADTVLKWRRSGPASWGVRYLGGRK
                     SFVFAPIGHSIGVSNATLRIEKGGASGSLIISRLGRVRVEMH"
     gene            complement(929..1519)
                     /locus_tag="VFU45_08305"
     CDS             complement(929..1519)
                     /locus_tag="VFU45_08305"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU5359107.1"
                     /translation="MAAIVLASISTSGRCRAQVPTAIPSGTVTSGALSFDGHSTLGDF
                     TGTTTTVRGAVRGAASIDSVSGWVEAPVNTLRTGNGKRDRDMMSSMEADRFPGIRYEL
                     SSVTRRGTRGDTLDLTLHGNFVIHGVTRPVDLQATAVFAPGQVRIQSSVPLDLNDYHI
                     EGLSKMLGILKMHPGILVHVDVTFGLAAPVPPRPGA"
     gene            complement(1566..1934)
                     /locus_tag="VFU45_08310"
     CDS             complement(1566..1934)
                     /locus_tag="VFU45_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5359108.1"
                     /translation="MDQEAADMMLRVLLVTDDAERVAPLAAALRATGCDVVVAAPGAD
                     EPDDRIDLVLTDPAMPPGTLEDAERRHISATLRHTQGNKRQAAHLLGIARSTLLAKVR
                     KYGLDAVGHDAREPAGDLAS"
     gene            2126..>3074
                     /locus_tag="VFU45_08315"
     CDS             2126..>3074
                     /locus_tag="VFU45_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5359109.1"
                     /translation="MGFPDLPRLGLGERIQDALLVREVDQRTYQGDKTMTTLTLGNAS
                     GTIGSAPFWEQDQHLVAGVKAGDVVQVIGEVGAYKDRRQLQVTSLRVLPRGKVDWGVL
                     LPSAGDVSRYWETIDKWRAEIAGPRLRAVISLFYDDEDFRTRYGQCPASTVGHHAELG
                     GLIKHTVEVASIGRHIAKVARADVDLVTAGVLLHDIGKLEAYRWDGLFAPTDKNALYG
                     HVVLGALMLDDAVRSAEPMPCTEQELDILMHLVLAHHGRLEFGAPVEPKTLEAEIVHY
                     ADNASAKTASFADALADPSNFIGEELVSAKGIWALDKRRA"
BASE COUNT          462 a         1070 c         1100 g          442 t
ORIGIN      
        1 tgggcaaccg gtccacgaac ttgaacgcgg cctgcgcctg gaagcggaac ccctcccgca
       61 cgtccgccgc ggcttcgccg ccgagcgtcg cggcccggtc gaccgcccgc agcgtcaggt
      121 atgcgccaag cgccagctcg gcgccgcgat cgtcccccgc ctcgcgcgcg gaagcggcac
      181 gggccaggaa cacggcatgc ggctcgatgg cggcctcggc cgccggggac tggtcgaaca
      241 gggtgctgga atggatcacg ccacgcagcc tagcaagggg cgggccaaaa gggggtgacc
      301 gagagtatcg ctcccggcgc cccggacggc aagggagccg cgttcctcgg gccatcggcg
      361 cggattccgg atgacggtcg gtggcgtcct gaccgctacc cttgcagcat gcgacagggc
      421 ttcaccctcc ccgaagtggc cattaccctg gccctcgtcg gcatcctgac gggattcgcc
      481 gcgccttcgc tgatggccgc ggccgaccgc ggcgccatcc ggatggggct cgcccagctg
      541 cggggggcgc acgaagaggc ccggctcacc gcggtcctcg acgaccgcgt gtcggtgctc
      601 accatcgact ccctggccat caccctgcgg accgtcctcg gcgccgacac cgtgctcaag
      661 tggcgccgca gcggccccgc ctcctggggc gtgcgctacc tgggagggcg gaagtcattc
      721 gtctttgccc cgatcggcca ctcgatcggg gtctccaacg ccacgctccg catcgaaaag
      781 gggggcgcga gcggtagcct gatcatctcc cggctgggac gggtccgggt ggaaatgcac
      841 tagcgccccc tctccgtccg tcaggatgga gagggggtcg ggggtgaggt ggacggatgg
      901 gcggaccgac tggtcgaccg aaggcgcctc aggcaccggg ccgaggtggc accggcgccg
      961 ccagcccgaa cgtcacgtcc acgtgcacca ggatgcccgg atgcatcttg aggatgccga
     1021 gcatcttgga gagtccctcg atgtggtaat cgttgaggtc gagcgggacg gaactctgga
     1081 tccggacctg gccgggggcg aacaccgccg tggcctgcag gtcaacgggg cgggtcacgc
     1141 cgtggatgac gaagttcccg tggagcgtca ggtcgagcgt atcgccgcgc gtcccgcgcc
     1201 gggtgacgct cgaaagctcg taccggatgc cggggaaccg atccgcctcc atggacgaca
     1261 tcatgtcgcg atcgcgcttg ccgttgccgg tccggagcgt gttgaccggc gcctcgaccc
     1321 atccgctcac gctgtcgatc gacgccgcgc cgcggaccgc gccgcggacg gtcgtggtgg
     1381 tgccggtgaa gtcgccgagg gtggagtggc cgtcaaaact gagcgcgccg gaggtgaccg
     1441 tcccggacgg gatggcggtc ggcacctgcg cccggcagcg cccggaggtc gagatggacg
     1501 caagcacgat cgcagccatg aaccatcgga cggacactcg ggctcctctg cggggtatga
     1561 cccttctacg acgccaggtc ccccgccggt tccctcgcat cgtggcccac ggcgtcgagg
     1621 ccgtatttcc gcaccttggc cagcagggta gagcgcgcga tgccgagcag gtgggcggcc
     1681 tgccgcttgt tgccctgggt gtgccggagg gtggcggaga tgtgccggcg ctcagcgtcc
     1741 tcgagggtgc ccgggggcat cgcggggtcg gtcagcacca ggtcgatccg gtcatccggc
     1801 tcgtcggcgc cgggcgccgc gacgaccacg tcacacccgg tggcgcggag cgcggcggcc
     1861 agcggggcca cccgttcggc gtcgtcggtg accagcagca cgcggagcat catgtcggca
     1921 gcctcctgat ccatctgcgg aaagctacgt cggcagctgc aggtgcgacg accttcccca
     1981 gcatcgcggt gtgagggggt gacgtacgcc agcccggacg atgcacgccc tcggctcggg
     2041 gcccgcaccg ccccgccgga cccgccgatc gcacggcggc ggctttgccg ctaatgtttc
     2101 cccctcctct ccgggtggag ctccaatggg atttcccgac cttccgcgcc tcgggctcgg
     2161 tgagcgcatc caggacgcgc tcctggtgcg cgaagtggac cagcgcacct accagggcga
     2221 caagacgatg accaccctga cgctcggcaa cgcgtccggg accatcggct ccgcgccgtt
     2281 ctgggagcag gaccagcacc tggtggccgg ggtcaaggcc ggcgacgtgg tgcaggtcat
     2341 cggcgaggtc ggggcctaca aggaccggcg ccagctccag gtgacctcgc tccgcgtgct
     2401 cccccgcggc aaggtcgact ggggcgtgct gctcccctct gccggtgatg tctcccgcta
     2461 ctgggagacc atcgacaagt ggcgggccga gatcgccggc ccccgccttc gggcggtcat
     2521 ttccctgttc tacgacgacg aggacttccg gacccgctac ggccagtgtc cggccagcac
     2581 cgtgggccac cacgccgagc tgggcgggct catcaagcac accgtggagg tggcgagcat
     2641 cgggcgccac atcgccaagg tcgcgcgggc cgacgtcgac ctggtgacgg cgggcgtcct
     2701 cctgcacgat atcgggaagc tcgaggcgta ccgctgggac gggctcttcg caccgaccga
     2761 caagaacgcg ctctacggcc acgtggtgct cggggcgctg atgctcgacg acgccgtgcg
     2821 gagcgccgaa ccgatgccct gcaccgagca ggaactcgac atcctgatgc acctggtgct
     2881 ggcgcaccat ggccggctcg agttcggggc accggtggaa cccaagaccc tcgaagcgga
     2941 gatcgtgcac tacgccgata acgccagcgc caagacggcg agcttcgccg acgcgctggc
     3001 agatccgtcg aacttcattg gcgaggagct ggtgagcgcc aaggggatct gggcgctcga
     3061 caagcggcgg gcgt
//
LOCUS       DASUAP010000309         6136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_677992, whole genome shotgun sequence.
ACCESSION   DASUAP010000309 DASUAP010000000
VERSION     DASUAP010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677992"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VFU45_08320"
     CDS             complement(<1..1448)
                     /locus_tag="VFU45_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU5359110.1"
                     /translation="MQLTTLDWAIIAASVVISFLPALFFYKRAGTSTAEFFTSGRAAP
                     WWLIGVSMVATTFSTDTPNLVTNMVRENGVADNWLWWSFLLTGMMTVFFYARLWRRSG
                     VLTDLEFYELRYSGKAATFVRGFRAVYLGLFFNCVIMATVNLAAAKIANVVLGLPMWE
                     TMLICSVITIFFASVSGLWGVLVTDSIQFSITMTGTFAVAYFAVKQPQIGGLSGLWSK
                     VSPATLNLLPDFSNWGVALSVIIIPITIQWWSVWYPGAEPGGGSYIAQRMLASKSEKD
                     ALTGTLLFNVMHYALRPWPWIIVAVASTVIYPDLASIAHQFPHLDPKLLGNDIAYPAM
                     LRFLPAGFLGLMIAGMLAAYRSTIETHLNWGTSYLVHDFYRRFLKRDGTERHYVFMGR
                     LTTALLMVCAALLTFELDTAKEAFDLILSVGAGTGLIYLLRWFWWRVNAWSEVSAMVS
                     SFLVALGFFVAGKMGMAVPSYVSLIATVATTTV"
     gene            complement(1724..2773)
                     /locus_tag="VFU45_08325"
     CDS             complement(1724..2773)
                     /locus_tag="VFU45_08325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5359111.1"
                     /translation="MTLLCGRAAFIALVLAAPLAAQTPTVSRADVARVLSTLADDSMQ
                     GRLTGTPGADRAAAFVAAEMARIGLTPLGDSGYFQRVALESVTFPPRPGAPAGALPRA
                     RLVLLHELSDLDTVPAAQRRHGQNVLGEIEGSDPVLRHEIVLVDAHYDHLGVGRPVHG
                     DSIYNGADDDASGVTTVLEVARAIAAGPRPKRTIVFAAMTGEEVGLLGTRWYLAHPAV
                     PIAQMAANLEVEMIGRPDSLAGGPGKAWLTGYERSTLGDELRSHGIPIVPDPRPEQHF
                     FERSDNIAFARAGVPAHTLSSFGLHADYHEPSDDFAHVDLDHMTKVIDAAVAAVRLMA
                     DGPRPEWHAGGRPTE"
     gene            2957..5362
                     /locus_tag="VFU45_08330"
     CDS             2957..5362
                     /locus_tag="VFU45_08330"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5359112.1"
                     /translation="MQLSRLAAVPLLLSLGGSLSAQSAVAPKLLPPPPGATYSGWDHE
                     TSLHIPRIDATIQVDGSLDEAAWASAARLTGFSQYSPTDGARANDSTVVLVWYSPTAI
                     YFGIRAYEAHGQPHGTLANRDNIFSDDNVQIYLSTFDDGRQATVFAVNPLGVQADGVL
                     VESGKSNGNSVFGNAIIGGREPIDLSPDYVFQSKGQVTPWGYEVEVRIPFKSLRYQSA
                     RSQDWGLNIERDVQHDGTTQTWTPARRAAASFLGQSGTLAGITDIHRGLVMDLNPELT
                     GRRDGATDPGTGAYAYSNTGPDIGGNVRWGVTNNLTLNATAKPDFSQVESDAGQLGFD
                     PRQALFYPEKRPFFLDGLEFFQSPYQLVYTRQIVQPVGALKLTGKAAGTNLGVIAAVD
                     DQADGLVPGHNPVFGIVRAQRDLGAGSKLGLVYTDRTDGPDRNQVGALDGRFVFGKVY
                     SAQFQAGMSHDVQNGAATDAPIWAFSFRRAGRTFGWNSAFNGVSDKFRTRSGFISQAG
                     VANALVDPSYTVYGGKDRWFQRITGDVSLGGVWQYEHLIHGQGLQTRQLHFNFSSVLK
                     GGWQVGGGYFFESFGFDSGLFANYRLLVPNGAATDTVRFTGQPTIPNSEFVVQVGTPT
                     FRTFDANGFILRGHDENYYEWASGDLVVLQAGVNWRPNANLRVGATYSWQQVNRRTDG
                     TLVNVGRVPRLTVEYQVSRPFFVRVVGQYIQNETDSLRDNSRTELPIYIAQPDGSLLR
                     AGPRKTDLFHADVLLSYQPGPGTVVYAGYGSNQTEPSAFRFQDFTRLNDGLFVKFSYL
                     FRM"
     gene            complement(5452..>6136)
                     /locus_tag="VFU45_08335"
     CDS             complement(5452..>6136)
                     /locus_tag="VFU45_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5359113.1"
                     /translation="AVTISFNLAPNVALGDAVSEVQKLANATLPASISTAFSGTAQAF
                     KDAQSGLLGLLVLAVLVIYLVLGILYESFIHPLTILSGLPFAGFGALLTLYIFRMDVS
                     LYAFVGIIMLVGLVKKNAIMMVDFAVEAEKSGKSAREAIVEACSVRFRPIMMTTFAAL
                     MGTLPIAIGSGTGAESRRPLGVAVVGGLAFSQFITLYVTPVVYTYFDEFQHWLATRRK
                     RAVVAAEAH"
BASE COUNT         1030 a         2160 c         2059 g          887 t
ORIGIN      
        1 acggtggtgg tcgccacggt cgcgatcagc gacacatagc tcggcaccgc catccccatc
       61 ttccccgcca cgaagaagcc gagcgccacc aggaacgagc tcaccatcgc gctcacctcg
      121 ctccaggcgt tgacccgcca ccagaaccag cgcagcaggt agatgagccc cgtgccggcg
      181 ccgactgaga ggatcaggtc gaacgcctcc ttggcggtgt cgagctcgaa ggtgaggagc
      241 gccgcgcaca ccatcaggag cgccgtcgtg agccggccca tgaacacgta gtgccgctcg
      301 gtgccgtccc tcttgaggaa gcggcggtag aagtcgtgca ccaggtagct ggtgccccag
      361 ttgaggtggg tctcgatcgt cgaacggtag gccgccagca tcccggcgat catcagcccc
      421 aggaagcccg ccggcaggaa tcggagcatc gccgggtagg caatgtcgtt gccgaggagc
      481 ttggggtcga gatgggggaa ctggtgcgcg atcgacgcca ggtccgggta gatgaccgtc
      541 gacgccaccg ccacgatgat ccagggccag gggcggagcg cgtagtgcat cacgttgaag
      601 aggagcgtgc cggtgagggc gtccttctcc gacttcgacg ccagcatccg ctgtgcgatg
      661 tagctcccgc cccccggctc ggcgccggga taccagaccg accaccactg gatcgtgatc
      721 gggatgatga tcaccgagag cgccaccccc cagttggaga agtcggggag gaggttgagg
      781 gtggccgggc tcaccttgct ccagagcccc gacaggccgc cgatctgggg ctgcttcacc
      841 gcgaagtacg ccaccgcgaa ggtgcccgtc atcgtgatcg agaactggat cgagtccgtc
      901 accagcacgc cccagagccc cgacaccgac gcgaagaaga tcgtgatcac cgagcagatg
      961 agcatcgtct cccacatcgg gaggccgagc accacgttgg cgatcttggc ggcggcgagg
     1021 ttgaccgtcg ccatgatgac gcagttgaag aagaggccca ggtagacggc gcggaacccg
     1081 cggacgaagg tggccgcctt gcccgagtag cgcagctcgt agaactcgag gtcggtgagc
     1141 accccggagc gccgccagag ccgggcgtag aagaacacgg tcatcatccc ggtcagcagg
     1201 aacgaccacc agagccagtt gtcggcgacg ccgttctccc ggaccatgtt ggtgacgagg
     1261 ttcggggtgt cggtgctgaa ggtcgtcgcc accatcgaca ccccgatcag ccaccacggc
     1321 gccgcgcgcc cggaggtgaa gaattccgcc gtgctggtcc ccgcgcgctt gtagaagaag
     1381 agggcgggga ggaacgagat gacgacggat gccgcgatga tggcccagtc gagggtcgtc
     1441 agctgcatgg gggcgccgga ggtgggggga ggcttcggat gacggatgta aaataggggg
     1501 accggcggct acacgcgcag gggcggcgca cgcgccgccc gcagaggcca accctcgcac
     1561 ggaccttcac cctcacccgt ggccttcacc ctcacccctg acccctctcc cgcagggaga
     1621 agggccggga gggagggata agcggacatc ggccgggaat gagggtcaca ccacgcggct
     1681 ttgtacgcgg cagttcacgc ggcccttcac ccgccccacc cgcctactcc gtcggccgac
     1741 cccccgcgtg ccattccggc ctcggcccgt cggccatcag ccgcaccgcc gccaccgccg
     1801 catcgatcac cttggtcatg tggtcgaggt ccacatgggc aaagtcgtcc gacggctcgt
     1861 ggtagtcggc gtggagcccg aagctcgaga gcgtgtgcgc cggcactccc gcgcgggcga
     1921 aggcgatgtt gtcgctccgc tcgaagaagt gctgttcggg gcgcgggtcg gggacgatcg
     1981 ggatcccgtg agacctgagc tcatcgccca gcgtggaacg ctcatagccc gtgagccacg
     2041 ccttgccggg gccgccggcg agggagtcgg gccggccgat catctcgacc tcgaggttgg
     2101 ccgccatctg cgcgatcggg accgccggat gcgccaggta ccagcgcgtc cccaggaggc
     2161 ccacctcctc cccggtcatc gcggcgaaga cgatcgtccg cttcggacgc ggccccgcgg
     2221 cgatcgcccg cgcgacctcg agcaccgtcg tgacgcccga ggcatcgtcg tccgcgccgt
     2281 tgtagatcga gtcgccgtgc accggccggc cgacgccgag atggtcgtag tgcgcgtcga
     2341 ccaggacgat ctcgtgcctg aggaccgggt cgctcccctc gatctcgccc agcacgttct
     2401 ggccgtggcg gcgctgggcg gcggggaccg tgtcgaggtc cgagagctcg tggaggagga
     2461 ccaggcgcgc gcgcggcaac gcccccgccg gcgcgcccgg acgcggcgga aaggtgacgc
     2521 tctcgagcgc cacgcgctgg aagtagccgc tgtcgcccag cggcgtgagc ccgatccgcg
     2581 ccatctcggc ggcgacgaag gccgccgccc ggtcggcgcc gggcgtgccc gtcagccggc
     2641 cctgcatcga gtcgtcggcg agggtggaga ggacccgggc gacgtcagcg cgggagacag
     2701 tgggagtctg cgccgcgagc ggcgcggcaa gaacgagggc gatgaacgcg gcacgcccgc
     2761 agaggagggt cacggaaggt ccagtggaag gggacaggtg cttcgacggc ggtcgggaat
     2821 gtatgcttgt gtgaacgagg agtcatccgg cggccggccc cgccgccatg ctgaatcctg
     2881 cgcggcgcat ccttcgtacg caccacggtg cggcgcaggt ggcgccgcgt tccaccttgg
     2941 cgccgaggct ccacgagtgc agctctcccg acttgccgcc gtcccgctcc tgttgtctct
     3001 cggcggctcc ctctcggccc aatcggccgt cgcgccgaag ctcctcccgc caccgccagg
     3061 cgccacctac tcggggtggg atcacgaaac gtcccttcac attccccgca tcgacgccac
     3121 catccaggtc gatggttcgc tcgacgaggc ggcgtgggcg tccgccgcgc ggctcaccgg
     3181 gttttcccag tacagcccga ccgacggcgc ccgcgccaac gactcgaccg tcgtcctcgt
     3241 ctggtactcc ccgaccgcta tctacttcgg catccgcgcc tacgaggcgc acggtcagcc
     3301 gcacggcacc ctggccaatc gcgacaacat cttctccgac gacaacgtcc agatctacct
     3361 cagcacgttc gacgacgggc gccaggccac ggtgttcgcc gtcaacccgc tcggcgtcca
     3421 ggccgacggc gtgctggtcg agtcgggaaa gtcgaacggc aacagcgttt tcggcaatgc
     3481 gatcatcggc ggccgcgagc cgatcgacct gagccccgac tacgtcttcc agtccaaggg
     3541 gcaggtgacc ccgtgggggt acgaggtcga ggtcaggatc ccgttcaaga gcctccgata
     3601 ccagtcggcc cgctcgcagg actggggcct caacatcgag cgcgacgtcc agcacgacgg
     3661 caccacccag acctggaccc ccgcccggcg ggccgcggcg tccttcctcg ggcagtcggg
     3721 cacgctggcg gggatcaccg atatccatcg cgggctggtg atggacctga accccgaact
     3781 caccgggcgc cgggacggcg ccaccgatcc cggcaccggc gcatacgcct actccaacac
     3841 cggccccgac atcggcggca acgtgcgctg gggcgtcacc aacaacctga ccctcaacgc
     3901 cacggccaag ccggacttct cccaggtgga gtccgacgcc ggccagctcg gcttcgaccc
     3961 gcgccaggcg ctcttctacc ctgaaaagcg gcccttcttc ctcgacgggc tcgagttctt
     4021 ccagtcgccc taccagctcg tctacacccg ccagatcgtg cagccggtgg gcgcgctcaa
     4081 gctgaccggc aaggccgccg gcaccaacct cggcgtcatc gccgccgtcg acgaccaggc
     4141 cgacgggctg gtgccgggcc acaaccccgt cttcggcatc gtccgcgccc agcgcgacct
     4201 gggcgccggc tcgaagctgg ggctcgtcta caccgaccgc accgacggcc ccgaccggaa
     4261 ccaggtgggc gcacttgacg gaaggttcgt cttcggcaag gtgtacagcg cgcagttcca
     4321 ggccgggatg agccacgacg tgcagaacgg cgctgcgacc gacgccccga tctgggcctt
     4381 cagcttccgg cgcgccggcc ggaccttcgg atggaactcg gcgttcaacg gggtgtcgga
     4441 caagttccgc acccggagcg ggttcatcag ccaggcgggc gtcgccaacg cgctcgtcga
     4501 cccgagctac accgtgtacg gcggcaagga ccgctggttc cagcgcatca ccggcgacgt
     4561 gtcgctcggc ggcgtctggc agtacgagca cctgatccac gggcagggac tgcagacccg
     4621 gcagctgcac ttcaacttct cgtcggtgct caagggcggc tggcaggtcg ggggcgggta
     4681 cttcttcgag tcgttcggct tcgactcggg gctcttcgcg aactaccggc tcctcgtgcc
     4741 caacggtgcc gccaccgaca cggtgcggtt caccggccag cccaccatcc cgaacagcga
     4801 gttcgtcgtc caggtcggta cgccgacgtt ccgcacgttc gacgccaacg ggttcatcct
     4861 gcggggccac gacgagaact actacgagtg ggcctccggc gacctcgtgg tgctgcaggc
     4921 cggggtcaac tggcgcccca acgccaacct gcgggtgggc gccacgtact cgtggcagca
     4981 ggtcaaccgc cgcaccgacg gcacgctggt caacgtgggg cgggtgcccc ggctgacggt
     5041 ggagtaccag gtctcgcggc cgttcttcgt ccgggtggtg ggacagtaca tccagaacga
     5101 gaccgactcg ctgcgcgaca actcgcgcac cgagctcccc atctacatcg cccagcccga
     5161 cggctcgctg ctgcgcgccg ggccgcggaa gaccgacctc ttccacgccg acgtcctgct
     5221 gagctaccag cccggccccg gcaccgtggt ctacgccggg tatggcagca accagaccga
     5281 gccctcggcg ttccggttcc aggacttcac ccgcctcaac gacggcctct tcgtcaagtt
     5341 cagctatctg ttccggatgt gacggcaagc ggtgagcggt cagaagtgag cggggggctc
     5401 cgtcaccggt cggggccccc cgctcacctc tgacttctca ccgctcaccg atcagtgcgc
     5461 ctccgctgcc accactgctc gcttccgccg cgtcgccagc cagtgctgga actcgtcgaa
     5521 gtaggtgtag accaccggcg tcacgtagag cgtgatgaac tgcgagaacg cgaggccgcc
     5581 caccaccgcc acgccgagcg gtcggcggga ttcggcgccg gtcccggagc cgatggcgat
     5641 cgggagcgtg cccatcagcg ccgcgaaggt cgtcatcatg atcggccgga atcgcacgct
     5701 gcacgcctcg acgatcgcct cgcgggcgct cttgccggat ttctccgcct cgaccgcgaa
     5761 gtccaccatc atgatggcgt tcttcttgac cagccccacc agcatgatga tccccacgaa
     5821 cgcgtagaga ctcacgtcca tccggaagat gtacagcgtc aggagcgcgc cgaacccggc
     5881 aaagggaagc ccggaaagga tcgtgagcgg gtggatgaag ctctcgtaga ggatgcccag
     5941 caccaggtag atgaccagga ccgcaagcac caggagcccg agcaggccgc tctgggcgtc
     6001 cttgaacgcc tgggcggtgc cggagaatgc ggtcgagatg ctggccggca gggtggcgtt
     6061 ggcgagtttc tggacttcgg acacggcgtc gcccagggcc acgttcgggg ccaggttgaa
     6121 ggagatcgtc accgcc
//
LOCUS       DASUAP010000310         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1168660, whole genome shotgun sequence.
ACCESSION   DASUAP010000310 DASUAP010000000
VERSION     DASUAP010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168660"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="VFU45_08340"
     CDS             <1..1666
                     /locus_tag="VFU45_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEU5359114.1"
                     /translation="DAPTTVGVIRGVETLLQLVSADSTGYFLPAVAISDAPRFRWRGL
                     LLDVGRHFEPVEVVERTLDGMAAAKLNVLHWHLSEDQGFRVESKRFPRLQEFGSDGLY
                     YTQDQIRAVVAYAADRGIRVVPEFDMPGHSTSWFVGYPQYASGKGPYAIQRTWGVFDP
                     AFDPTRESVYRFLDGFIGEMSGLFPDAFWHIGGDEVPGTEWKANPRIQAWMKAHHIKD
                     NAALQAHFNRRLIAILARHHKQVVGWDEILNPALPKTAVIQSWRGTNYLGDAAKQGYR
                     GILSAPYYLDHIDQAGQHYLADPLPASAGLTDAEAARVLGGEACMWSEYVGPETVESR
                     IWPRMLAVAERFWSPASVTDVADMYRRLGIVSVELGDLGFDHLDHTRRFVRRMMNGAP
                     GGAALERLLAVTEPGNFGQRADAMSWTQQTPLVRLVDAAVPDPASRRVYGDLATRAIA
                     GDTAARSELDRQFAELRQLPAGVRTAARSAPLAAEGIPAADMVAAVGAVGLEADSFLA
                     RGATMPVATRDSALSTLRDAAKPYGMLRITIVDAVQALVEAAGGKP"
     gene            1852..>3753
                     /locus_tag="VFU45_08345"
     CDS             1852..>3753
                     /locus_tag="VFU45_08345"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HEU5359115.1"
                     /translation="MSGAPGRLPTGQRLDPAGVTTTVGQMPLGMALAPDGRRVALLLS
                     GYREQGLQVTDRQGQVTQTLPQVAAFIGLVFSPDGRSLYASGGNLDLVYRYAWAGDSA
                     VLADSIVLGPRARDGNATHYPAGLAFSPDGRSLYVAEDLADSLAVVDVATRQVVQRIG
                     LDRYPYGVAVTPDGTVFVSCWNADSVTVLRTTPDHRLGIAGKVAAGRHASALVLDRAG
                     TRLFAASGSTDRVSVIDTKALRVVAVLRDTVPRGVREGATPNALALSPDGTRLYAAEG
                     DANAVAVFELSRGTADTSVAAASDRFVGRIPTGWYPVSLAVAGDTLLVASGKGLGTAP
                     DPDGPRPGIARVKRGPGADRQYTLSQLDGALSIVPLGEAPLASLTRRVYAANGWNDRA
                     RRHRYPPFRHVIYVIRENRTYDQVLGDLPRADGDPSLVFFGRDVTPNAHALAERFGVF
                     DRFFVNAEVSGDGHNWSTAAYATDYTQKSVPVSYSAHGGRSYDYEGTNRDSIPTDDVA
                     APAQGFIWDLAEQAGASFRDFGERVAEEERDGKKVYVGAEKFLRTHTDPAYPGFDLAI
                     PDQRRADLWLAQFEQWVLADSMPALQTILLPRDHTAGGAAGSNTPRAMVADNDLALGR
                     IVEAVSKSPF"
BASE COUNT          546 a         1335 c         1341 g          531 t
ORIGIN      
        1 cgatgcgccc acgaccgtgg gggtgatccg cggcgtcgag acgctcctcc agctcgtctc
       61 cgccgattcg acgggctatt tcctgccggc cgttgccatc tcggatgcgc cgaggttccg
      121 gtggcgggga ttgctgctgg acgtgggacg ccacttcgag ccggtggagg tggtcgagcg
      181 cacgctcgac gggatggccg ccgccaagct caacgtgctg cactggcacc tctccgagga
      241 ccaggggttc cgggtcgaga gcaagcgctt tccacgactc caggaattcg gctccgacgg
      301 gctctactac acgcaggacc agatccgcgc ggtggtggcg tacgctgccg accgcggcat
      361 ccgggtggtg ccggaattcg acatgccggg ccactcgacc agctggttcg tgggctatcc
      421 gcagtacgcc agcggcaagg gtccctacgc catccagcgc acctgggggg tgttcgaccc
      481 cgcgttcgac ccgacgcggg agtcggtgta ccgcttcctg gacggcttca tcggtgagat
      541 gagcgggctc tttcccgacg cattctggca catcgggggc gacgaagtgc cggggaccga
      601 gtggaaggcg aatccccgga tccaggcgtg gatgaaggcg catcatatca aggacaacgc
      661 ggcgctgcag gcgcacttca accggcggct catcgccatc ctcgcacgcc atcacaagca
      721 ggtggtgggg tgggatgaga tcctgaatcc ggcgctcccg aagaccgccg tcatccagtc
      781 gtggcgcggc accaactacc tcggcgatgc tgcgaagcaa gggtaccgcg gcatcctgtc
      841 cgccccctat tacctcgacc acatcgacca ggcggggcag cactacctgg ccgatccgct
      901 cccggccagc gccggactca ccgacgccga agctgcgcgg gtgctcgggg gtgaggcgtg
      961 catgtggagc gagtacgtgg gccccgagac ggtggagagc cgtatctggc cccggatgct
     1021 cgcagtggcg gagcggttct ggagcccggc gtcggtgacc gacgtggccg acatgtaccg
     1081 ccggctcggc atcgtgtcgg tggagctcgg cgacctgggc ttcgaccacc tcgaccacac
     1141 ccggcgcttc gtccggcgga tgatgaacgg cgcgcccggc ggcgcggcgc tcgagcggct
     1201 cctggccgtg accgagccgg gcaacttcgg ccagcgggcc gacgcgatga gctggaccca
     1261 gcagaccccg ctcgtccggc tggtggacgc cgccgtgccc gacccggcat cgcgccgggt
     1321 gtatggcgac ctggcgaccc gcgcgatcgc cggcgacacc gcggcgcgga gcgagctcga
     1381 ccgccagttc gctgagctgc gccagctgcc ggcgggcgtc cggaccgccg cccgcagcgc
     1441 accgctcgcg gccgagggga tccccgccgc ggacatggtg gccgccgtgg gtgccgtggg
     1501 cctcgaggcc gacagcttcc tggcccgcgg cgccacgatg cccgtcgcca cccgcgactc
     1561 ggcgctgtcc acgctgcgcg acgcggcgaa gccctacggg atgctccgca tcaccatcgt
     1621 cgacgcggta caggcgctgg tcgaggcggc gggagggaag ccgtgagtgc gagtgaagag
     1681 tcaagagtga agagtgaaga gtcaaaggtg aagagtgaag agtcaaaggt gaagagtgaa
     1741 cagggagaga tgcgcgacgg acgccgacgc atcctcttca cgctttactt ttcactcttc
     1801 actgccctca ctgtcgcgtc cgcctgcgcc ccccgcaccg ccgcgccccc cgtgagcggc
     1861 gcacccggac ggctccccac cggccagcgg ctcgatcccg ccggtgtcac gacgacggtg
     1921 ggacagatgc cgctcgggat ggcgctggct cccgacggcc ggcgcgtggc gctcctcctg
     1981 agcgggtacc gggagcaggg gctgcaggtg accgatcgtc aggggcaggt cacccagacc
     2041 ctcccgcagg tggcggcgtt catcgggctc gtgttctcgc ccgatggccg gtcgctctac
     2101 gcctccggcg gcaacctcga cctcgtgtat cgctacgcct gggccggcga cagcgccgtc
     2161 ctggccgact cgatcgtcct gggcccccgc gccagggacg ggaacgccac ccactacccg
     2221 gcggggctcg cgttctcgcc cgacggccgc tcgctctatg tcgcggagga cctggccgac
     2281 tcgctcgccg tcgtggacgt ggcgacgcgc caggtggtgc agcgcatcgg cctggatcgc
     2341 tatccctacg gcgtggcggt gacgccggac ggcacggtgt tcgtctcgtg ctggaatgcc
     2401 gactcggtga cggtgctgcg cacgacgccc gaccatcggc tcggcatcgc cggcaaggtg
     2461 gccgcgggcc ggcacgcctc ggcgctcgtg ctcgaccgcg cggggacccg cctcttcgcg
     2521 gcgtcgggga gcaccgatcg cgtgtcggtg atcgacacga aggcactccg ggtcgtcgcg
     2581 gtgctgcgcg acaccgtccc ccgcggcgtg cgggaaggcg cgacgccgaa cgcgctcgca
     2641 ctctcaccgg acggcacgcg gctctacgcc gccgaggggg acgcgaacgc cgtggcggtg
     2701 ttcgagctgt cgcgcgggac cgcagacact tccgtggcgg cggcgagcga ccgattcgtg
     2761 ggccggatcc ccaccgggtg gtatccggtg tcgctcgccg tggcggggga cacgctcctc
     2821 gtggccagcg gcaaggggct cggcaccgcg cccgatcccg acgggccgcg gcccggcatc
     2881 gcccgcgtga agcgcggccc cggcgccgac cggcagtaca cgctgagcca gctcgacggg
     2941 gcgctctcga tcgtgccgct cggcgaggcg ccgctcgcgt cgctcacccg gcgggtctac
     3001 gccgccaacg gctggaacga ccgcgcccgg cggcatcgct atccgccgtt ccggcacgtc
     3061 atctacgtga tccgcgagaa ccgcacctac gaccaggtcc tgggcgacct ccccagggcc
     3121 gacggcgacc cgtcgctcgt cttcttcggg cgcgacgtga ccccgaacgc ccacgcgctg
     3181 gcggaacggt tcggcgtgtt cgaccgcttc ttcgtgaacg cggaggtgag cggcgacggg
     3241 cacaactggt ccaccgcggc ctacgcgacg gactacaccc agaagtcggt ccccgtcagc
     3301 tactcggcgc atggcggacg gagctacgac tacgagggga ccaaccgcga ctcgatcccc
     3361 accgacgacg tggcggcgcc ggcgcaggga ttcatctggg acctggcgga acaagcgggg
     3421 gcatcgttcc gcgacttcgg cgaacgggtg gcggaggagg agcgggacgg gaagaaggtc
     3481 tacgtcggcg ccgagaagtt cctccggacg cacaccgacc cggcctatcc gggcttcgac
     3541 ctggccattc ctgaccagcg ccgggccgac ctctggctgg cgcagttcga gcagtgggtc
     3601 ctggccgact cgatgccggc gttgcagacg atcctgctcc cgcgcgacca caccgccggc
     3661 ggcgccgcgg gcagcaacac cccgcgcgcg atggtggccg acaacgacct ggcgctgggc
     3721 cgcatcgtcg aggcggtgtc gaagagcccg ttc
//
LOCUS       DASUAP010000311         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_801232, whole genome shotgun sequence.
ACCESSION   DASUAP010000311 DASUAP010000000
VERSION     DASUAP010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801232"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFU45_08350"
     CDS             <1..192
                     /locus_tag="VFU45_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359116.1"
                     /translation="STGFCNQASVIGFGASVDYRLDRSWRLQTSFEPTYQSCTRGYNQ
                     WKPSTAYQVGFDALWDREF"
     gene            192..1127
                     /locus_tag="VFU45_08355"
     CDS             192..1127
                     /locus_tag="VFU45_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU5359117.1"
                     /translation="MPRALVITNPAASRTRARTVAAIMRVFARGGWQAEVLATGGPGD
                     ARRFAEAGVRDQVDVVAVFGGDGTTMQAAAALVGTGVALGVIPGGTGNLLAGNLRLPS
                     SPIRAAQAIVRGRPRPLDLGRMERDGGTQYFAVACGAGYDARVMAETGAAEKHKWKFA
                     AYVATTLRLLPELKPARFTLTVDGVEHETDASMLLIANCGEVIPPFVKLRSGIVPDDG
                     LLDVVAVRASNFSESVRAVWDLVSEGPRRGRDEFVGYARGREIRVRSEPAEPVQLDGE
                     PHGETPFTATVVPGALKVMVGNGGRVSRVPRPESR"
     gene            1139..2488
                     /locus_tag="VFU45_08360"
     CDS             1139..2488
                     /locus_tag="VFU45_08360"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5359118.1"
                     /translation="MANSVLLIDDDVDVLRSIGAYFERIGYEVTRELSGEAGLATFDR
                     NRPDVVILDLGLPGMDGMEVLEHLRQRGAAVVLLTGQGDVGTAVRAMQLGAENFLTKP
                     VDMAHLAAATERVADKVRLRRVNESLVMHDAPGGRGLDSLGGSGQMLELAHQVALLAQ
                     SDRTTVLLTGESGTGKGWVARLIHDLGPRAREPFVEVNCAGLTATFLDSELFGHEKGA
                     FTDAKDRKQGLFEIADRGTIFLDEIGDLAPELQPKLLKVLETKTFRRLGGTREITVDV
                     RLVAATNRDLHDLVEQGRFREDLFYRLSVMPLRLPAVRDRTREDRLALITRILTDLRR
                     ELPDGPGGLNTEAMERLLQYAWPGNVREMRNVLERALILGRGSAMLSVEHLPGEFRAR
                     PGPGDRRHTPLSLEELERQHIDRTLKHHAGNRTRAAVELGISRATLINKIKRYGISS"
     gene            2499..2690
                     /locus_tag="VFU45_08365"
     CDS             2499..2690
                     /locus_tag="VFU45_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359119.1"
                     /translation="MNQDQPAGHKPGEKDAMRCRACGNEERASEGYPCERCGTFICLI
                     CTFRGVTLCAECAKQGSHA"
BASE COUNT          427 a          964 c         1036 g          405 t
ORIGIN      
        1 agcaccgggt tctgcaacca ggcctcggtg atcggcttcg gcgcgagcgt ggattaccgg
       61 ctcgatcgca gctggcggct ccagacctcg ttcgagccga cctaccagtc ctgtacccgc
      121 ggctacaacc agtggaagcc gtccaccgcg taccaggtcg gcttcgacgc gctctgggac
      181 cgggagttct gatgccgcgg gcgctcgtga tcaccaatcc tgccgcctcc cgcacccgtg
      241 cgcggaccgt ggcggcgatc atgcgcgtct ttgcccgcgg cggatggcag gccgaggtgc
      301 tcgccaccgg cgggccgggc gatgcgcggc gcttcgccga ggccggcgtg cgggaccagg
      361 tggatgtggt cgcggtcttc ggcggcgacg ggaccaccat gcaggcggcc gcggcgctgg
      421 tgggaaccgg cgtggcactg ggcgtgattc ccggcggcac cgggaatctc ctggccggca
      481 acctgcggct gccgtcatca ccgatccgcg ccgcccaggc gatcgtgcgc ggccggccac
      541 ggccgctcga cctgggccgg atggagcggg acggcggcac gcagtacttc gccgtcgcct
      601 gcggggcggg gtacgacgcg cgggtcatgg cggagaccgg tgccgcggag aagcacaagt
      661 ggaagttcgc ggcctatgtc gccaccacgc tgcgcctgct gcccgagctc aagccggccc
      721 gcttcaccct cacggtggac ggggtcgagc acgagaccga cgcctcgatg ctgctgatcg
      781 ccaattgcgg cgaggtcatc ccgcccttcg tgaagctccg ctccggcatc gtgccggatg
      841 acgggctgct cgacgtggtg gcggtgcgcg cctcgaactt cagcgagagc gtgcgggcgg
      901 tctgggacct ggtgagcgaa ggcccgcgcc gcggccgcga cgagttcgtg gggtacgcgc
      961 gcggccggga gatccgggtg cggagcgagc cggccgagcc ggtgcagctg gatggcgagc
     1021 cccacggcga gacgccgttc acggcgacgg tggtgccggg ggcgctgaag gtgatggtgg
     1081 ggaacggcgg acgcgtgtct cgagtcccgc gtcccgagtc caggtaacgg agcttctcat
     1141 ggcgaattca gtccttctca tcgacgacga cgtggatgtc ctgcgctcga tcggcgccta
     1201 cttcgagcgg atcggctacg aggtgacgcg cgagctgagc ggcgaggcgg ggctcgccac
     1261 cttcgaccgg aaccggccgg atgtggtgat cctcgacctc ggactcccgg gcatggacgg
     1321 catggaggtg ctggagcacc tgcgccagcg cggcgcggcg gtggtgctgc tcaccggcca
     1381 gggtgacgtc gggacggcgg tgcgcgccat gcagctgggt gccgagaact tcctgaccaa
     1441 gccggtggac atggcgcacc tcgccgccgc caccgagcgg gtggcggaca aggtccgcct
     1501 gcgccgggtc aacgagtccc tggtgatgca tgatgccccc ggcggccgcg gactggactc
     1561 cctcggcggc tcgggccaga tgctggagct ggcgcaccag gtggcgctgc tggcccagag
     1621 cgaccgtacc accgtgctcc tcaccggcga gagcggcacc ggcaagggct gggtcgcgcg
     1681 gctgatccat gaccttggac cccgcgcgcg cgagccgttc gtcgaagtga actgcgccgg
     1741 cctcaccgcc accttcctcg acagcgagct gttcgggcat gagaagggcg cgttcaccga
     1801 tgccaaggac cggaagcagg gactgttcga gatcgcggac cgcggcacca tcttcctgga
     1861 cgagatcggc gacctcgccc cggaactcca gcccaagctg ctgaaggtgc tggagaccaa
     1921 gaccttccgg cggctgggcg gcacacggga gatcaccgtg gacgtgcggc tcgtggccgc
     1981 caccaaccgc gacctgcacg acctggtaga gcagggacgg ttccgcgaag acctgttcta
     2041 ccggctgagt gtcatgccgc tccggctgcc cgccgtgcgg gatcgcaccc gggaggatcg
     2101 cctggcactg attacccgca tcctcaccga tctccgccgc gagcttccgg acggccccgg
     2161 cggcctcaat accgaggcca tggaacgcct gctgcaatac gcctggcccg ggaacgtgcg
     2221 cgagatgcgg aacgttctgg agcgggcgct gatcctcggc cgcggcagcg ccatgctgag
     2281 cgtggagcac ctgccgggcg agttccgcgc ccggcccggc cccggcgacc ggcgccacac
     2341 gcccctcagt ctcgaggagc tggaacggca gcacatcgac cgcacgctca agcatcacgc
     2401 cggcaatcgc acccgtgccg cggtggaact cggcatctcc cgcgctacgc tgatcaacaa
     2461 gatcaagcgc tacggcattt ccagctgagg tgggcatcat gaatcaggac cagccggccg
     2521 ggcacaagcc cggggagaag gacgccatgc gctgccgcgc ctgcggcaac gaggagcgcg
     2581 catcggaggg ctatccctgt gagcggtgcg ggacgttcat ctgcctgatc tgcaccttcc
     2641 gcggcgtgac gctctgcgcc gagtgcgcga agcagggctc gcacgcatga gcagtgtggt
     2701 ggtgacggcc gccgtcgccc cggtccatgc cgagccctcg gtgcgggcgg aacaggtgac
     2761 ccagctggtg ctgggagaga ccgcgagcat ccgcgaggac cacggtgagt ggctggcgat
     2821 ccggagcgac gt
//
LOCUS       DASUAP010000312        11544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_568821, whole genome shotgun sequence.
ACCESSION   DASUAP010000312 DASUAP010000000
VERSION     DASUAP010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568821"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFU45_08370"
     CDS             complement(<1..397)
                     /locus_tag="VFU45_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5359120.1"
                     /translation="MTRRSPTSPDLILAAARREFSAHGLAGARTEAIARRAGVNKQLL
                     HYYFSTKAQLYRATLDAAATELAGSLARLPLVGLTAVERLRRLLRGQFDLLAEREEVT
                     RLLVRSDGDGGWVATAVTPVTTLLTEGQAT"
     gene            complement(417..2042)
                     /locus_tag="VFU45_08375"
     CDS             complement(417..2042)
                     /locus_tag="VFU45_08375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5359121.1"
                     /translation="MTDPLDLVLAQLARDYVAEAPSRIGELRKDLAALEAGESDALDS
                     LRARFHRLAGSGGSHGLPDVSDIARQAEQWILTHPIAGPDAYGTILAAVEALSAAFDR
                     AASGIEAAAPAAPSARHVEFAWHALILGASPEVGAKLSGPLESAGFTVRIAPHGTETP
                     DEAPAHANPDLIVVMHAPDGPDPYALASAWTSRRQVRPRAVVLIEPLEGADRLRAAAA
                     GVDAVFDQAAVETELPEFARALARIGQPPGSVLLVEDDPAAAQLITAILEQANLRVTR
                     CGTVREAEQVIGGMYPDLFLLDIRLPDGDGFTLARYIRHDPRFALTPLIFLTAATGPE
                     SQMEALRCGGDDFLAKPAHPQLLTQLVVSRIERGRRIREMAHRDGLTGLLNHATLMAE
                     LDHAVESWHRRKDPFSFVMVDLDHFKRINDRHGHLAGDRVLVHVARILQQVARASDLL
                     GRYGGDEFGLILPGNGVDGAMSFADRARAAIANDPPSDPAGAPIPVHLSVGIAVYPED
                     GDRASVLVQAADRALYQAKRRGRDQIIRAQDVD"
     gene            complement(2039..2728)
                     /locus_tag="VFU45_08380"
     CDS             complement(2039..2728)
                     /locus_tag="VFU45_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5359122.1"
                     /translation="MPSAIDLTPFGFTPTESRMYAALVRHGPGTGYALAQATGLARAN
                     AYSALEGLVAKGAARVDGERPKRYRPEAPSTLMVRLSNAQGEALDRLGNALANVSGGD
                     GAAVVDLTSARGLLQLLTREIARAHERVWLCAPPEAYPILAPALRRARAQVPAVDLRS
                     TGLVTLDFAEVGGLAGTQDWPGEPIVCVVDRRSAVLAARSGDTVQGQWSSTPAFVAGA
                     AAAFLQLRGNA"
     gene            complement(2786..3985)
                     /locus_tag="VFU45_08385"
     CDS             complement(2786..3985)
                     /locus_tag="VFU45_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359123.1"
                     /translation="MVARCPDCPGPAIFAPACRPEFRRSPRPGAALELPTHHRIPLTW
                     LLEHGGESIRFRSLKEFAPAGSVPAEELEAAQLAITESKAALAITKRQKDTGAWGGNI
                     MGLGPSPKDGIKEPGTVTQYRRLLQLEWPRQGRPFRLADRVLYRLLSRDEDPALLFEY
                     QKLVKAEPIAEPWARGVIREAACAALAEAGYVEDPRLRGAAHKIASAISHFLRSPLAA
                     KPFVRAGAHTALHPEAYPPSWYSVAMMAAMPNLQRERAGFTERLGAYLAHPAPKKAFV
                     VQIGKKVARPTHLLLGDPIEADAKGHPKDLPLALHYIELMARIGALQFAPVATRVLAR
                     LIKDCDEQGIWHPKNLRSQPKLISKIPQHAFPLVAESKSQESKQVDVTFRLALIAKAL
                     GWQLEIA"
     gene            complement(4048..4950)
                     /locus_tag="VFU45_08390"
     CDS             complement(4048..4950)
                     /locus_tag="VFU45_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359124.1"
                     /translation="MRFSLVMAAAVLVAPLTAQAPPPPLPSAPAPSWHRDAVPFKDTR
                     IPESSGVVVSRTTPGVLWTFNDSDNPPDIFATDTAGRALGRWRILGVNDRDWEALAPG
                     PGPCRGGHCLYIGEIGDNDSQYPAVALFRVDEPVATGRDDSIPVRDVLHFRYPDGPHD
                     AESLIIDSTGDAYVITKPRVHWPEVFRIPASSWGRRDTVVAELTDSLALDPRSGVEMW
                     VTDASLAPDGRTVAVRTYGYLYLYHLSARGHLTPVGRSPLCSLHGLGAQGEGVAWLGP
                     DTFALTSERFFILPASIAVARCRG"
     gene            complement(4959..6188)
                     /locus_tag="VFU45_08395"
     CDS             complement(4959..6188)
                     /locus_tag="VFU45_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEU5359125.1"
                     /translation="MTAALAAFVSLPLAAQLRAKGDKGWGDELASSRLATAPARQRLP
                     AATATTRDSIDWPAPPITPPPSIVHAIYLNAWTFGSRRFYDLVRLADTTEINAFVVDV
                     KDDTGYLTYRSSVPTAEAIGADRAIRAPDTRHRLAVLRAHNIYPIARIVVAKDPLLAR
                     MRPAWSVQDAGGGLWHDRLKFAWVDAYNDSVWIYAAQLGAEAVHEGFGELQFDYVRFP
                     DEPRSRMESARFTARRNNESTRDAVARHLRLLRSLVHPLGVPLAIDIFGMTASADRDL
                     GIGQVWEDLASTADVVQPMVYPSHYYGGFYNIQHPNSEPYAVVHRALEDAERRSAPLR
                     HAAEIRPFLQAFTLGRPRYTPVEIRDQIRAAADVGIQSWVLWNPRGVYDPEIFRPRRA
                     PAAIATARIAAVRTVPR"
     gene            6411..6647
                     /locus_tag="VFU45_08400"
     CDS             6411..6647
                     /locus_tag="VFU45_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:6441..6443,aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HEU5359126.1"
                     /translation="MKLTIQFCVVUNYEPRAAGLAAELRQAFPGIEISMVKSSGGVFE
                     VTLDGELLFSKKAAGRHAAPGEVLELVRKRVGGR"
     gene            6711..7790
                     /locus_tag="VFU45_08405"
     CDS             6711..7790
                     /locus_tag="VFU45_08405"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEU5359127.1"
                     /translation="MRIALNTGGGDAPGLNAVIRAVVLSAHARGWQTYGILRGFDGLL
                     KNDGVVNLSPDRVRGITHLGGTILGTTNRGNPFRWATTDASGREVEVDRSDELIAAFQ
                     ASGFDAFITIGGDGSLKIGHDLWKKGLPVVGVPKTIDNDVSGTQITFGFDTAVSTATE
                     AIDKLHSTAESHNRVMVVELMGRDAGWIALNSGISASADVILIPEIPYDIDTVCAKVR
                     DREASGRHFSIVVVAEGAKPKGGRHHLLEGAGVGTVERLGGIAAVVAKEIGEQTGKET
                     RSLVLGHIQRGGSPTTFDRLLALRFGAAAVRAVADREFGVMVGLASPTIVRVPLADVI
                     GRPKLVPLDSDTVMTARELGICLGD"
     gene            7848..8258
                     /locus_tag="VFU45_08410"
     CDS             7848..8258
                     /locus_tag="VFU45_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359128.1"
                     /translation="MRATNVQTRWRAATPTLLTVLGWTTGMATFLSIWVVAVSVVRRS
                     FYFDKYHMSTWQVLGSYYAASVIAALALRLLWPLTRWRAGAFAVGTVIGTMTYGVIMF
                     ALGGWSWSSAWLATIPGTLVGGGLGAHWFSKPPL"
     gene            complement(8489..8839)
                     /locus_tag="VFU45_08415"
     CDS             complement(8489..8839)
                     /locus_tag="VFU45_08415"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU5359129.1"
                     /translation="MPGKEPVLGAATAAGRLGAIPSWRIEDNCLVRTYETDGWPSTLL
                     VVGAIAFVAEAANHHPDLSVSWPRVVVRLQTHSAGGLTDKDFELAQKIEETVTWWPSR
                     GWPGLEGNPKRFVK"
     gene            complement(8857..9354)
                     /locus_tag="VFU45_08420"
     CDS             complement(8857..9354)
                     /locus_tag="VFU45_08420"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU5359130.1"
                     /translation="MSGTFRVDVKKDYLSFSSAHFITFRGHKCESLHGHNYRVGVSVE
                     GAVDAEALFVLDFAVLKEVTKPMVDAIDHKVLLPGRNPKVSISHEGDRTLVTVFGESR
                     YAFPARDVAVLPVSNTTAEMLAEHFATEVRAALDRQGVSHLSLIEIEIEESPGQSATY
                     RLPLR"
     gene            9403..10002
                     /locus_tag="VFU45_08425"
     CDS             9403..10002
                     /locus_tag="VFU45_08425"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU5359131.1"
                     /translation="MAEGEKARLRREGLARRDAEPDRDAKGKAIQERLQATAVYGRAR
                     TVAAYVGVRSEVPTLPLLESMLTSGKRVVLPVVEGDDIRLIRLEAISELAPAVFGLLE
                     PGPAVRDRLDRRVQPAELDLMLVPGVAFDRKGGRIGYGKGYYDRLLARTRSGTPTVGL
                     AFEAQLIAAVPMGPADYHLKHLVTERMFYTFDHVRRHGR"
     gene            10113..10562
                     /locus_tag="VFU45_08430"
     CDS             10113..10562
                     /locus_tag="VFU45_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359132.1"
                     /translation="MRATPLLGLAVLACGLVATRPAAAQRVSVGMTFGGGPVWGRVYV
                     GPRGGYRWYHRDMDRRPVVVDAYGDYPRDVEVERLWVPRGRAWGWWRQHGYQEADLWV
                     DDGGRYYDRDDDRPGRLREVTVYQRDGRYYRAGGDDRDRGDRHGRDD"
     gene            10664..>11544
                     /locus_tag="VFU45_08435"
     CDS             10664..>11544
                     /locus_tag="VFU45_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5359133.1"
                     /translation="MLPARNHQLPLDVEAFRRARHPRGRVIVIAPTRAACETIELALG
                     LDLDTVLERQHGADLRRLAAGGRGFGIVAGTGTGKTLAIRPIAETILGEPLRAGVVNR
                     ERESTPETPGWNVVIVTTGIARRWFEDGLITGVDTIVVDEIHQTSAELELCLALGKRA
                     RCRFIWLSATVDPSFYARYLESADVIESSAFDPALRAKVQVQARRVEEFLDDKYVRRL
                     IRDRRGVAVFVPTRAEVEQLATGLGERWPRLSAAFYHGGEPIRVIRPFLDGEVERPFL
                     LAMTAAGQSALNIRGLDT"
BASE COUNT         1697 a         3910 c         4210 g         1727 t
ORIGIN      
        1 cggtggcctg cccttcggtg agaagcgtcg tcaccggcgt cacggcggtg gccacccagc
       61 cgccgtcgcc atcggaccgg accagcagcc gggtcacctc ctcccgctcg gccagcaggt
      121 cgaactggcc gcggagcagg cgccggaggc gctccacggc cgtgaggccc acgagcggca
      181 gccgcgccag gctccccgcc agctcggtcg cggcggcgtc gagcgtggcg cggtagagct
      241 gggccttggt ggagaagtag tagtggagga gctgcttgtt gacgccggcc cgccgggcga
      301 tggcctcggt gcgggcgccc gccaggccgt gggccgagaa ttcccgccgc gccgcggcca
      361 ggatcaggtc gggggaggtg gggctccggc gggtcatcgg gcgggatccg cgccggtcag
      421 tcgacgtcct gcgcgcggat gatctggtcg cggccccgtc gcttggcctg gtagagcgcc
      481 cggtcggcgg cctgcaccag gacgctcgcc cggtcgccgt cctccggata gacggcgatc
      541 cccacgctca ggtggacggg gatcggcgcc ccggccggat cgctcggcgg gtcgttcgcg
      601 atcgccgcgc gcgcgcggtc cgcgaacgac atcgcgccgt cgacgccgtt gccagggagg
      661 atcagcccga actcgtcgcc gccgtagcgt cccagcaggt cgctggcccg ggccacctgc
      721 tgcaagatgc gcgcgacgtg gaccagcacc cggtccccgg ccaggtggcc gtgacggtcg
      781 ttgatccgct tgaaatgatc caggtccacc atcacgaacg agaacgggtc cttgcgccga
      841 tgccacgact ccaccgcgtg gtcgagctcg gccatcagcg tggcgtggtt gaggaggccc
      901 gtgagcccgt cgcgatgcgc catctcgcgg atccggcggc cacgctcgat ccggctcacc
      961 acgagctggg tgagcaactg gggatgcgcc ggcttggcca agaagtcgtc gccgccgcag
     1021 cgcagcgcct ccatctggga ctccgggccg gtcgccgcgg tgaggaagat cagcggcgtc
     1081 agcgcaaagc gggggtcgtg ccggatgtag cgcgcgagcg tgaagccgtc gccgtccggc
     1141 agccggatgt cgagcaggaa gaggtcggga tacatcccgc cgatcacctg ctcggcctcc
     1201 cgcaccgtac cgcaccgggt gacccgcagg ttggcctgct ccaggatggc ggtgatgagc
     1261 tgggccgccg ccggatcgtc ctcgaccagg agcacgctgc ccggcggctg gccgatgcgg
     1321 gcgagcgccc gcgcgaattc ggggagctcc gtttccacag cggcctggtc gaatacggcg
     1381 tcgacccctg ccgccgccgc ccgcaggcgg tcggcgcctt cgagtggctc aatcagcacc
     1441 accgcccgcg ggcgcacctg gcgccgggag gtccatgcgc tcgccagggc gtaggggtcg
     1501 gggccgtcgg gtgcgtgcat cacaacgatg aggtccggat tggcatgcgc cggcgcttcg
     1561 tcgggcgtct cggtgccgtg cggcgcgatc cgcaccgtga agccggcgct ctcgagcggg
     1621 ccggacagct tcgccccgac ctccggggaa gcgccgagga tgagcgcatg ccaggcgaat
     1681 tcgacgtgcc gggccgaggg cgcggccggg gcggcagcct cgatacccga ggcggcgcgg
     1741 tcgaatgcgg cgctgagcgc ctcgaccgcg gccaggatcg tcccgtacgc gtccggcccg
     1801 gcgatcggat gggtcaggat ccactgctcg gcctggcggg cgatgtcgga tacgtcgggg
     1861 agcccgtgcg atccgccgga gcccgcaaga cggtggaacc tcgcccggag tgagtcgagc
     1921 gcgtcggatt cccccgcctc gagcgccgcg aggtccttgc gcagctcgcc gatccgtgac
     1981 ggcgcctcgg cgacgtagtc tcgggcgagc tgcgccagca ccaggtcgag cgggtcggtc
     2041 acgcgttccc ccgcaactgc aggaacgcgg cggcggcgcc ggccacgaat gcgggggtgg
     2101 aactccactg gccctgcacg gtatcgccgc tgcgtgcggc cagcaccgcg ctgcggcggt
     2161 cgacgacgca cacgatcggc tcaccgggcc agtcctgggt cccagcgaga ccgccgacct
     2221 cggcgaagtc gagggtcacc aggccggtgg agcggaggtc cactgccggc acctgggccc
     2281 gggcccgccg gagcgccggc gccaggatcg gatatgcctc gggcggggcg cagagccaga
     2341 cccgttcgtg cgctcgcgcg atctcccggg tcaggagctg cagcaggccc cgggctgagg
     2401 tcaggtcgac cacggccgcg ccgtcgccac cgctcacgtt ggccagcgcg ttgccgagcc
     2461 ggtcgagcgc ctcgccctgg gcattgctca gccgcaccat cagcgtcgac ggcgcctcag
     2521 gccgatagcg cttgggccgc tcgccatcga cgcgggcagc gcccttggcc accaggcctt
     2581 cgagcgcgct gtaggcgttg gcgcgcgcca accccgtcgc ctgggccagg gcgtagccgg
     2641 tgcccgggcc gtggcgcacc agggccgcgt acatccggct ctccgtcggg gtgaacccga
     2701 acggggtcag gtcgatggcg gaaggcatat aggagtattg cctacgacta ctcgccggtc
     2761 aatgccgcgc ccggctggcg cggactcagg cgatctcgag ctgccagccc agcgccttcg
     2821 cgatgagcgc caggcggaac gtcacgtcca cctgcttcga ctcctgcgac ttgctctcgg
     2881 ccaccagcgg gaaggcgtgc tgcgggatct tgctgatgag cttgggctgg gagcggaggt
     2941 tcttggggtg ccagatcccc tgctcgtcgc agtccttgat caggcgggcg aggacgcggg
     3001 tggccaccgg ggcgaactgg agcgcaccga tccgcgccat caattcgatg taatggagcg
     3061 ccagcggaag gtccttcggg tgtcccttgg cgtcggcctc gatcgggtcg cccagcagca
     3121 ggtgggtggg gcgggcgacc ttcttcccga tctgcaccac gaacgccttc ttcggggccg
     3181 gatgcgccag gtaggcgccg agccgctcgg tgaacccggc ccgctcccgc tgcaggttgg
     3241 gcatcgccgc catcatggcc accgagtacc agctcggcgg ataggcctca gggtgcagcg
     3301 cggtgtgtgc gccggcccgc acgaagggct tggccgcgag cgggctccgc aggaaatggg
     3361 agatggccga ggcgatcttg tgggccgcgc cccgaaggcg cggatcctcg acgtatcccg
     3421 cctccgccag cgcggcgcac gccgcctccc ggatgacgcc gcgggcccac ggctccgcga
     3481 tcggctccgc cttcaccagc ttctggtact cgaagaggag cgcgggatcc tcgtcccggg
     3541 agagcagccg gtagagcacc cggtccgcca ggcggaaggg gcgcccctga cgcggccatt
     3601 ccagctgcag cagccgccga tactgggtga ccgttcccgg ctccttgatg ccgtccttgg
     3661 gcgacggtcc gaggcccatg atgttgccgc cccaggcgcc ggtgtccttc tggcgcttgg
     3721 tgatcgcgag cgcggccttc gactcggtga tggcgagctg cgccgcctcg agttcttcgg
     3781 ccggtaccga gccggcgggg gcgaattcct tgagggaccg gaatcggatc gactccccgc
     3841 cgtgctccag gagccacgtg agcgggatgc gatgatgggt cgggagttcc agagcggctc
     3901 ctggacgtgg ggaacgtcgg aattcgggcc ggcaagcggg cgcgaatata gcggggcccg
     3961 ggcagtcagg acaacgagcg accatgcctc cgcgacttgg aacgtgctat ctggttgtgt
     4021 gacaaagagt taactggcta ctgtgattca accgcgacat cgggccacgg cgatcgaggc
     4081 cggaaggatg aagaaccgct cgctcgtgag ggcaaaggtg tcggggccaa gccaggcgac
     4141 gccctcgccc tgcgccccca gcccgtggag gctgcagagc ggggagcgcc ccacgggcgt
     4201 gaggtggcca cgggcgctca ggtgatagag gtacaagtac ccgtaggtcc ggaccgccac
     4261 ggtccggcca tcgggcgcga gggaagcgtc cgtgacccac atctcaactc cagagcgcgg
     4321 gtcgagcgcc agggaatcgg tgagctcggc gacgaccgtg tcgcgacgac cccacgacga
     4381 ggcggggatg cggaacacct cgggccagtg caccctcggc ttggtgatca cgtaggcgtc
     4441 gcccgtcgag tcgatgatga gcgattcggc gtcgtgcggg ccgtccgggt accggaagtg
     4501 gagcacgtcg cggacgggga tggagtcgtc ccgcccggtg gcgacgggct catccacgcg
     4561 gaagagcgcg accgccggat actgggaatc gttgtcgccg atctcgccga tgtagaggca
     4621 gtggccgccg cggcaggggc cgggacccgg ggcgagcgcc tcccagtcgc ggtcgttgac
     4681 gccaaggata cgccaccggc ccagcgcgcg tcccgcggtg tcggtggcga agatgtccgg
     4741 tgggttgtcg gagtcgttga acgtccagag cacgccggga gtggtacggc tcaccaccac
     4801 gccggacgat tccgggatgc gagtgtcctt gaaggggacg gcgtcgcggt gccacgacgg
     4861 cgccggtgcg gacgggaggg gcgggggcgg ggcctgcgcg gtaagcggcg cgacgaggac
     4921 cgccgcggcc attaccaggg agaaccgcat cgaccgggtc agcggggcac ggtccgcacc
     4981 gccgcgatgc gcgcggtcgc gatggcggcc ggggcgcggc gggggcggaa gatctccggg
     5041 tcgtacacgc cgcgcgggtt ccagagcacc cagctctgga tcccgacatc ggcggcggcg
     5101 cggatctgat cgcggatctc caccggcgtg tagcgcggcc ggccgagcgt gaacgcctgc
     5161 aggaacggcc ggatctccgc cgcgtgccgg agcggcgccg atcggcgctc ggcgtcctcc
     5221 agggcgcggt gcaccacggc gtacggctcg ctgttcgggt gctggatgtt gtagaacccg
     5281 ccgtagtagt gactggggta caccatcggt tggacgacgt ccgccgtgct cgcgaggtcc
     5341 tcccacacct gcccgatgcc gagatcacgg tcagcgctcg cggtcatgcc gaagatgtcg
     5401 atggcgagcg ggacgccgag cggatgcacc aggctccgga gcagccggag atggcgcgcc
     5461 accgcgtcac gggtcgattc gttgttccgg cgggcggtga acctggcgga ttccatccgc
     5521 gagcggggct catcggggaa ccggacgtag tcgaactgga gctcgccgaa gccctcgtgg
     5581 accgcctcgg cgccgagctg tgccgcatag atccacaccg agtcgttata ggcatccacc
     5641 caggcgaact tgagccggtc gtgccagagg ccgccgccgg catcctgcac cgaccaggcc
     5701 ggccgcatgc gcgccaggag cggatccttg gcaacgacga tccgcgcgat cggatagatg
     5761 ttgtgcgcgc gcaggaccgc cagccggtgg cgggtgtcgg gcgcgcggat ggcacggtcg
     5821 gccccgatgg cctcggcggt cgggaccgac gatcgatacg tcaggtagcc ggtgtcatcc
     5881 ttgacgtcga ccacgaaggc gttgatctcg gtcgtgtccg ccaagcggac caggtcgtag
     5941 aagcggcgcg agccgaaggt ccacgcgttg aggtagatgg cgtggacgat gctgggcggc
     6001 ggcgtgatcg gcggggccgg ccagtcgatc gaatcccgcg tggtggcggt cgcggcggga
     6061 agccgctgcc gtgccggcgc cgtcgcgagc cggctcgagg cgagctcgtc cccccacccc
     6121 ttgtctccct tggcgcgaag ctgggccgcg aggggcaagg aaacgaacgc cgccaaggcg
     6181 gcggtcatcg gcaggagggc gctaagccgt gctggcatac cgtgatagct aacgttgccg
     6241 caccgggatg gacaaggggg gaagggcctc cgccgctggt cggcggtggg gggtgggggt
     6301 agattcgggg agctatcagc tatcagctat cagctatcag ctatcagcta tcagccaacg
     6361 gccaacggcc aacgactgtc ccgtacacca cccacgaccc gcgacctgcc atgaagctca
     6421 ccatccagtt ctgcgtcgtc tgaaactatg agccccgggc cgccggtctg gcggccgagc
     6481 tccggcaggc attccccggg atcgagatct cgatggtcaa gagttccggc ggcgtcttcg
     6541 aggtgaccct cgacggcgag ctgctctttt ccaagaaggc ggctgggcgg cacgccgcgc
     6601 ccggcgaggt gctcgagctc gtccggaagc gggtcggcgg ccgctagctg gtgtagattc
     6661 accgaatgcc cctccccctt accgctcacc gctcaccgct caccgcctag atgcgcatcg
     6721 cgctcaacac cggcggcggc gacgcgcccg gactcaatgc cgtgatccgc gccgtggtgc
     6781 tgtcggccca cgcgcgcggc tggcagacct acgggatcct ccgcggcttc gatggtctcc
     6841 tcaagaacga cggcgtcgtg aacctctctc ccgaccgggt gcgcggcatc acgcatctgg
     6901 gcggcaccat cctcggcacc accaaccggg gcaacccgtt ccgctgggcc acgaccgacg
     6961 ccagcggccg ggaggtcgag gtcgaccgct ctgacgaact gatcgccgcg ttccaggcga
     7021 gcggcttcga cgcgttcatc accatcggcg gcgacgggtc gctcaagatc ggccacgacc
     7081 tgtggaagaa gggcctgccg gtggtgggcg tcccgaagac catcgacaac gacgtgagcg
     7141 ggacccagat caccttcggc ttcgacaccg cggtgagcac cgccaccgag gccatcgaca
     7201 agctgcattc gacggcggag agccataacc gcgtgatggt ggtcgagctg atgggccggg
     7261 acgcaggctg gatcgcgctc aacagcggga tctctgccag cgccgacgtc atcctgatcc
     7321 cggagatccc gtacgacatc gacaccgtct gcgccaaggt acgcgaccgg gaggcgtcgg
     7381 gacggcactt cagcatcgtc gtcgtggccg aaggggccaa gcccaagggc gggcgccacc
     7441 acctgctcga gggggccggc gtcggcaccg tcgaacggct ggggggcatc gccgccgtgg
     7501 tggcgaagga gatcggcgag cagaccggga aggagacccg ctcgctggtg ctggggcaca
     7561 tccagcgcgg cgggtcgccg acgaccttcg accggctgct ggccctccgg ttcggcgccg
     7621 ccgccgtgcg ggcggtcgcc gaccgggagt tcggcgtgat ggtcggcctg gcctcgccca
     7681 ccatcgtgcg ggtgccgctc gccgatgtca tcgggcgccc gaagctggtc cccctcgact
     7741 ccgacaccgt gatgacggcg cgcgaactgg ggatctgttt gggggattag ctaaccgcta
     7801 tccgctatcc gctgggtgcg agttcggcag cgaccatcac ttctgtcgtg agagccacca
     7861 acgtgcaaac acgatggcgc gccgcaaccc ccacgctgct gaccgtgctt ggttggacaa
     7921 cggggatggc caccttcctt tcgatctggg tcgtggctgt atctgtcgtg cggcggagtt
     7981 tctatttcga caagtatcac atgagcacat ggcaagtcct gggctcttac tacgcggcta
     8041 gcgtcatcgc cgcgctggct ctccgtctgc tgtggccatt gacgcggtgg cgcgccggcg
     8101 cgttcgccgt aggcacagtg atcggcacca tgacatatgg cgtcatcatg tttgcgctcg
     8161 gtggctggtc ttggtcgtcg gcatggctcg ctaccatccc tgggacgctg gtcgggggtg
     8221 gtttgggcgc ccattggttt agcaagccac cgctttgata tcaggctgcc caaccaagtg
     8281 ctggggctgg cgggccttcc gggccggggc gcagcggaca actggatggt tcggggcggg
     8341 gaaggggaga cgttgaattc gttgctggtg atccagctcc cgcaaccaaa gtgcggcggt
     8401 caaggccact gcagccatcg acaggctgac gcatatcgcc gaccttccgg acggatagcg
     8461 gatagcggat agcggatagc ggatggccct acttcacaaa cctcttcgga ttcccctcga
     8521 gcccgggcca cccgcgcgac ggccaccagg tgacggtctc ttcgatcttc tgggccagct
     8581 cgaagtcctt gtcggtcagg ccgccggcgc tgtgggtctg aaggcgcacc accacgcgcg
     8641 gccagctcac ggacaggtcg gggtggtggt tggccgcctc ggccacgaac gcgatggcgc
     8701 cgaccacgag cagggtggag ggccacccgt cggtctcgta ggtgcgcacg aggcagttgt
     8761 cctcgatccg ccagctcggg atggcgccca ggcgcccggc ggcggtggcg gcgccgagca
     8821 ccggttcttt tcctggcatc gggctctcct tggggttcag cggagcggca ggcggtacgt
     8881 ggcgctctgg ccggggctct cctcgatctc gatctcgatg aggctcaggt gggacacgcc
     8941 ctggcggtcc agcgccgcac ggacctcggt ggcaaagtgc tcggcgagca tctccgcggt
     9001 ggtgttggac accgggagca ccgccacgtc gcgggcggga aaggcgtagc gcgactcacc
     9061 gaagacggtg accagcgtcc ggtccccttc gtgggaaatg ctcaccttgg ggttccgccc
     9121 tgggaggagc accttgtggt cgatggcatc caccatcggc ttggtcactt ccttgaggac
     9181 ggcaaagtcg agcacgaaga gggcctccgc atcgacggcc ccttccaccg acacacccac
     9241 ccggtaatta tggccgtgca gggactcgca cttgtggccc cggaaggtga tgaagtgcgc
     9301 cgagctgaag ctcaggtagt ctttcttgac gtcgacccgg aacgtaccgc tcaccggcag
     9361 cctcgctgag gaatggcccc acctgcgtga gggacaaaga taatggccga gggggagaag
     9421 gcgcggcttc ggcgtgaagg gctggcccgc cgggacgcgg aacccgaccg ggatgcgaag
     9481 ggaaaggcca tccaggagcg cctccaggcc acggcggtgt acgggcgtgc ccggaccgtg
     9541 gcggcgtacg ttggcgtgcg gagcgaggtg ccgaccctgc cgctcctcga gtcgatgctg
     9601 acgtcgggga agcgggtggt gctcccggtc gtcgagggcg acgacatccg cctcatccgg
     9661 ctcgaggcca tcagcgagct cgcgccggcg gtcttcggcc tcctcgagcc ggggccggcg
     9721 gtgcgcgatc ggctcgatcg ccgggtgcag cctgcggagc tcgatctgat gctggtgccc
     9781 ggggtcgcct tcgaccgcaa gggcggccgc atcggttacg ggaaggggta ttacgaccgg
     9841 ctcctcgccc gcacccgctc cggcacgccg acggtcgggc tcgcgttcga ggcgcagctc
     9901 atcgccgctg tccccatggg ccccgccgac tatcacctca agcacctggt gacggaacgg
     9961 atgttctaca ccttcgacca tgtccgccgg cacggacgct gacccgcctc caccgccccg
    10021 ccgttccttc atttctcctt gttccacaag cacttggcac gctggcacgg aagtcgcgac
    10081 ccgggtccgc atctcttgac cggagcgttc cgatgcgtgc gacaccattg ctgggcctgg
    10141 ccgtccttgc ttgcgggctc gtcgccaccc gacccgccgc ggcgcagcgc gtctcggtcg
    10201 ggatgacctt tggcggtggc ccggtgtggg gccgcgtgta tgtcggtccc cgcggcggct
    10261 accggtggta ccaccgtgac atggatcggc ggccggtcgt cgtggacgcc tacggcgact
    10321 acccccgcga cgtcgaggtg gagcgcctgt gggtgccgcg agggcgggcg tgggggtggt
    10381 ggcgccagca cggataccag gaggccgacc tgtgggtcga cgacggcggg cgctactacg
    10441 accgggacga tgaccgcccg gggcgcctgc gcgaggtgac cgtgtaccag cgcgacggcc
    10501 gctactatcg ggccggcggc gacgaccggg atcgcggcga caggcacggc cgcgacgact
    10561 agatagatcc tccccatcac ggagcgccgc acggcgccag cggtcatcca cggactcccc
    10621 gtcagcaatg gcggggagtc tgccgttccg cttgattctt ggggtgctcc ccgcccgcaa
    10681 ccatcagctc ccgctcgacg tcgaggcctt tcgtcgggcc cgccatcccc gcgggcgtgt
    10741 catcgtcatc gccccgacgc gcgccgcctg cgagacgatc gagctggcgc tcggactcga
    10801 cctcgacacc gtgctcgaac gccagcacgg cgccgacctc aggcgcctcg cggcgggggg
    10861 ccggggcttc ggcatcgtgg ccgggacggg gaccggcaag accctcgcca tccggccgat
    10921 cgccgagacg atcctgggcg agccgctccg cgccggggtc gtcaaccggg agcgggagtc
    10981 gacgccggag acgccggggt ggaatgtggt catcgtcacc accggcatcg cgcgccggtg
    11041 gttcgaggac ggtctcatca ccggcgtcga caccatcgtg gtcgacgaga tccaccagac
    11101 ctcggcggag ctcgagctct gcctggcgct gggcaagcgg gcgcgctgcc gattcatctg
    11161 gctgagcgcc acggtggacc cgtcgttcta cgcccgctac ctcgagagcg ccgacgtgat
    11221 cgagagctcg gccttcgacc cggcgctccg ggccaaggtg caggtgcagg cccggcgggt
    11281 ggaggaattc ctcgatgaca agtatgtgcg ccgcctgatc cgggaccggc gcggcgtggc
    11341 cgtgttcgtg ccgaccaggg ccgaggtcga gcagctcgct accgggctcg gcgagcggtg
    11401 gccccggctc tcggcggcgt tctaccatgg cggcgagccg atccgcgtca tccggccgtt
    11461 cctcgacggc gaggtcgaac gcccgttcct cctcgcgatg accgcggcgg ggcagtccgc
    11521 gctcaacatc cgcgggctcg atac
//
LOCUS       DASUAP010000313         4886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_909927, whole genome shotgun sequence.
ACCESSION   DASUAP010000313 DASUAP010000000
VERSION     DASUAP010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909927"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFU45_08440"
     CDS             complement(<1..292)
                     /locus_tag="VFU45_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359134.1"
                     /translation="MNRRELLAASVAFPGAAWLASCAGGAAGAGAAAPPPPLPPARDD
                     ERWWADVRRQFLIADGVFMNTGTFGASPRQVVDATVAHLTAFETVFHQMPLDG"
     gene            complement(500..961)
                     /locus_tag="VFU45_08445"
     CDS             complement(500..961)
                     /locus_tag="VFU45_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359135.1"
                     /translation="MRDLWIFLHLAGMTMWLGGGFAVMFAGIASKKFTREQLASTARL
                     FAVIHARVIAPGAVLVVLSGLVLTMQLMSALGAGAGGAISPWIMVMQGVGLVAAALVI
                     VVSVPASMKAARLDPVTQPGAFDALRVRLRVVGAISGILGAVALLAGAMYR"
     gene            complement(958..1386)
                     /locus_tag="VFU45_08450"
     CDS             complement(958..1386)
                     /locus_tag="VFU45_08450"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEU5359136.1"
                     /translation="MTTEPRIFSVEEAERALPLVRRIVLDLQAEYPLWREAVGRFELA
                     SAGHHPGAAGSGELVACQRDVTERAERISGYLAELDQVGCVFKGFEAGLVDFYALRED
                     RLIFLCWRLGEARVTHWHELDAGYAGRRPIDEPTLTGTVP"
     gene            complement(1383..1793)
                     /locus_tag="VFU45_08455"
     CDS             complement(1383..1793)
                     /locus_tag="VFU45_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5359137.1"
                     /translation="MRARDVMVKDPHVAVAGAMASEVAILFHRRDISVIPVVDDHRTR
                     RFLGVISDRDLVTRCLAEGRDPLHTRADQLMRTNACVVGPEQELDGFVLRMDQDPGDS
                     HLRATITVVDAGQKVIGFISHPELIAGLKVVGNA"
     gene            complement(1908..2759)
                     /locus_tag="VFU45_08460"
     CDS             complement(1908..2759)
                     /locus_tag="VFU45_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5359138.1"
                     /translation="MSVPARVVAIYGGGGAKALGHLGAERALREADLAPACYVGCSMG
                     AVIAACLAAGLTPDEVKTRADALAGRRIAVPDPLVLLFGVGRPALLRSGPLRAAFEAL
                     VPVRSFAELKAPLTVAVTDLGSGQLLYFGNGPGDRDAPLLDVLMATCALPLYFPPVIL
                     DGKRCADGGIRAVVPFAAATRMGADLVVAVDVGPGFDEAPHQPAQPLPAMVRYNDEAL
                     GIAMAQHTRDQLALWRATAGHPRLVYIRPQVERNATFNVARMSTYLEWGYAATRAALT
                     EAGFGAH"
     gene            complement(2756..3202)
                     /locus_tag="VFU45_08465"
     CDS             complement(2756..3202)
                     /locus_tag="VFU45_08465"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEU5359139.1"
                     /translation="MSFSRAQLTTLVMPDMQNILGDLFGGHLMALVDQAAAVAAIRHA
                     GGPAVTASIDRVDFRERIPVGALVTCDASVDYVGNSSMHITVEVYAEKVSTGERRHTH
                     TAHMVFVAIDEFGHPKRVPRLVAEAPEEKARYEKARVHAEEMKKHR"
     gene            3335..3703
                     /locus_tag="VFU45_08470"
     CDS             3335..3703
                     /locus_tag="VFU45_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU5359140.1"
                     /translation="MRDHTSLIAWQEAEAVAITAVRLSRTYWRPPARAIFDQLLRASV
                     SAQVNISEGYAWTDSPTFRRHLQIAYGSAVECGDLLRLLERTGIVPPAEIAPVSERCG
                     RCQRLILGLYHRVAGKRNAG"
     gene            complement(3738..>4886)
                     /locus_tag="VFU45_08475"
     CDS             complement(3738..>4886)
                     /locus_tag="VFU45_08475"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEU5359141.1"
                     /translation="QLVGFEGGKGFAGGSVLLSPRGETLLQGPVFEPAVLTAALDMGE
                     ITRARADQPLLSDLEHRLPHLLEGRDQTLGRSVVPELGARGERQGRIARPEDGGESRR
                     VGASERLSTDPLRIDPQLARTWLVEFIRDEVQRRRGFEHVVIGLSGGVDSALVAYLAA
                     EALGPDHVTAVRMPYRTSSPESLAHADLVIRRLGLRERTVDISAAVDGLVGAIGGEVD
                     SARRGNVMARMRMITLFDLSAALKALPLGTGNKSERLLGYFTWHADDSPPVNPIGDLF
                     KTQVWALARHLGVPEEIVAKPASADLIQGQTDEGDFGISYAKADLILHWLLSGLRPAE
                     IEPLGFTAAEVALVRGRLDSTHWKRRLPTVAMLSQTSIGEYYLRPVDY"
BASE COUNT          748 a         1697 c         1750 g          691 t
ORIGIN      
        1 tgccgtcgag cggcatctgg tggaagacgg tctcgaatgc ggtgaggtgg gccacggtgg
       61 cgtcgaccac ctggcgcggg ctggcgccga aggtgccggt gttcatgaag acgccatcgg
      121 cgatgaggaa ctggcggcgg acgtcggccc accagcgttc gtcgtcgcgg gcggggggca
      181 acggcggtgg cggcgcggcg gccccggcgc ccgcggcacc cccggcgcac gacgcaagcc
      241 aggcagcccc cgggaacgcc accgacgcgg cgagcagttc acgacggttc atgcgcgctc
      301 cagaggaggg tgggaccacg gagtgcacga aggaacacgg agacccacgg agaactgcgt
      361 tggctgtggt ctcgcgaatg gggctccgcg cgctccgcca cgctgaatgg cagttctccg
      421 cgcctctccg gccacgccgt gcccgagcct gccctgagcg cagcgaaggg tggtcccagt
      481 cagcttcggt acgtgaaccc taccgataca tcgcccctgc gagcagggcg acggcgccga
      541 ggatcccgga gatggcgccg accacccgga gccgcacgcg aagcgcgtcg aacgctcccg
      601 gctgggtgac gggatcgagc cgtgcggcct tcatcgacgc cggcaccgac accacgatga
      661 cgagcgccgc ggccaccagg ccgacgccct gcatcaccat gatccagggc gagatggcgc
      721 cccccgcccc ggcgccgagc gcggacatca gctgcatcgt gaggacgagg cccgagagca
      781 ccaccagcac cgcaccgggc gcgatgacgc gagcgtggat caccgcgaag aggcgcgctg
      841 tcgaggccag ctgttcccgg gtgaatttct tgctcgcgat gccggcgaac atgaccgcaa
      901 agccgccgcc gagccacatc gtcatcccgg caaggtgcag gaagatccag aggtctctca
      961 aggtacggtc cccgtcagtg tgggttcatc gatgggccgc cgcccggcgt agccggcgtc
     1021 cagttcgtgc cagtgcgtca cccgcgcctc gcccagccgc cagcagagga agatgagccg
     1081 gtcctcccgc agggcgtaga aatccaccaa ccccgcctcg aagcccttga acacgcaccc
     1141 cacctggtcg agctcggcaa ggtatccgct gatccgctcg gcgcgttcgg tgacgtcccg
     1201 ctggcacgcc acgagctcgc ccgacccggc cgccccggga tggtggcccg ccgaggcgag
     1261 ttcgaacctc cccacggcct cccgccacag cggatactcg gcctgcaggt cgagcacgat
     1321 ccggcggacg agcggcagcg cccgctccgc ctcctccacc gagaagatcc tgggctcggt
     1381 ggtcacgcgt tgcccacgac cttgaggccc gcgatcagtt ccggatggct gatgaatccg
     1441 atcaccttct ggccggcgtc gaccacggtg atcgtggcgc ggaggtggga atcgccgggg
     1501 tcctggtcca tccggaggac gaagccgtcg agttcctgct cggggcccac cacgcacgca
     1561 ttggtgcgca tcagctggtc ggcccgggtg tggagcgggt cccggccttc ggccaggcag
     1621 cgggtgacga ggtcgcggtc ggagatgacg ccgagaaagc gccgggtgcg gtggtcgtcg
     1681 accaccggga tcaccgagat atcccggcga tggaagagga tcgcgacctc gctcgccatc
     1741 gccccggcca ccgccacgtg ggggtccttc accatcacgt cccgggcgcg catgggcggc
     1801 tcgctggttg gggttgggaa gctatcagct atcagctatc agctatcagc ggtagtgctc
     1861 ccttctcacc tctcacgact cacgactcac gactcacgac tcacgactca atgtgccccg
     1921 aatccggcct cggtcaaggc cgcacgcgtg gccgcgtatc cccattccag gtaggtcgac
     1981 atccgcgcca cgttgaacgt cgcgttccgc tcgacctggg gccggatgta gacgaggcgc
     2041 gggtgaccgg cggtggcgcg ccagagggcc agctggtcgc gagtgtgctg ggccatcgcg
     2101 atgccgaggg cctcgtcgtt gtagcgcacc atggcgggga gcggctgggc gggctgatgc
     2161 ggtgcttcgt cgaacccggg ccccacatcc accgcgacga cgaggtcggc gcccatccgg
     2221 gtggcggcgg cgaacggcac gacggcgcgg atcccgccgt cggcacagcg cttgccgtcg
     2281 aggatcaccg gcgggaagta gagcgggagc gcgcaggtgg ccatcagcac atcgaggagc
     2341 ggcgcgtcgc ggtcgccggg accgttgccg aagtacagca gctggcccga gccgagatcg
     2401 gtgaccgcga cggtgagcgg cgccttgagc tcagcaaagc tccggaccgg caccagcgcc
     2461 tcgaacgcgg cgcggagcgg ccccgacctc aggagcgccg ggcgaccgac gccgaagagg
     2521 aggaccagcg ggtcgggaac ggcaatgcgc cggcccgcga gcgcatcagc ccgggtcttg
     2581 acctcgtccg gtgtgaggcc ggcggcgagg cacgcggcga tgaccgcccc catggagcat
     2641 ccgacgtagc aggcgggcgc aaggtccgcc tcgcggagcg cccgctcggc gccgaggtgg
     2701 ccgagggcct tggcgccgcc gccgccgtag atcgccacca cccgggccgg gacgctcacc
     2761 ggtgcttctt catctcttcg gcgtggaccc gcgccttctc gtagcgcgcc ttctcctccg
     2821 gcgcctccgc cacgaggcgc ggcacccgct tggggtggcc gaactcgtcg atcgccacga
     2881 acaccatgtg ggcggtgtgg gtgtggcgcc gctcgccggt gctcaccttc tcggcgtata
     2941 cctcgacggt gatgtgcatc gagctgttgc cgacatagtc gaccgaggcg tcgcaggtga
     3001 cgagcgcgcc caccgggatc cgctcgcgga agtccacccg gtcgatgctg gccgtgaccg
     3061 ccggaccgcc ggcgtggcgg atcgcggcga ccgcggcggc ctggtccacg agcgccatca
     3121 ggtgcccgcc gaagaggtcg cccaggatgt tctgcatgtc cggcatcacc agcgtggtca
     3181 gctgggcgcg ggagaagctc atggtgcggg agtcggggat catgtgccgg tgagcggtga
     3241 ggggtgagcg gtgagcgggc gcgcccgcgg ataaatgcgc cctgccgcgc cgatcaggtt
     3301 atgcccggga caggggccgt ggataaagtg ggtcatgcgg gaccatacct cactcatcgc
     3361 atggcaggaa gcggaagccg tcgcgatcac ggccgtgcga ctcagtcgta cttattggcg
     3421 cccgcccgca cgggcgatct tcgaccagct cctgcgggca tccgtgtccg cgcaggtcaa
     3481 catctccgag ggttacgcct ggactgacag tccgacgttc cggcgtcacc tgcagatcgc
     3541 ctatggatcc gcggtcgaat gtggggacct gctgcggttg ctcgaacgca ccgggatcgt
     3601 gccgcccgcc gagatcgcgc cggtgtccga gcgctgcggc cgttgtcagc gcctcatcct
     3661 cggcctgtac caccgcgttg ccgggaagcg caatgcgggc tagggccccg ctcacctctt
     3721 acagctcacc gctcactcta atagtcgacc ggtcggaggt aatactcccc gatcgacgtc
     3781 tgggagagca tcgccacggt cgggagccgg cgcttccagt gggtcgagtc gagccggccg
     3841 cgcaccaggg ccacctcggc cgcggtgaag ccgagcggct cgatctccgc ggggcggagc
     3901 ccggagagga gccagtggag gatcaggtcg gccttggcgt agctgatccc gaagtcgccc
     3961 tcgtcggtct gcccctggat caggtcggcg ctggcgggct tggcgacgat ctcctcgggc
     4021 acgccgagat gccgcgccag cgcccagacc tgggtcttga agaggtcgcc gatcgggttg
     4081 acgggggggc tgtcgtcggc gtgccaggtg aagtagccga ggaggcgctc gctcttgttg
     4141 ccggtgccga gcgggagcgc cttgagcgcc gcggagaggt cgaacagcgt gatcatccgc
     4201 atccgcgcca tcacgttccc gcggcgggcg ctgtcgacct ccccgccgat ggcgccgacc
     4261 agcccgtcga cggcggcgga gatgtccacg gtgcgctccc ggagcccgag tcgccggatc
     4321 acgaggtccg cgtgggccag gctctcgggg ctcgaggtgc ggtacggcat ccggaccgcg
     4381 gtgacgtggt cgggtccgag cgcctcggcg gcgaggtacg ccaccagggc ggaatcgacg
     4441 ccgccggaga ggccgatcac cacgtgctcg aacccgcgcc ggcgctggac ctcgtcgcgg
     4501 atgaactcca ccagccaggt gcgcgcgagc tggggatcga tccggagcgg gtctgtggac
     4561 agacgctccg acgctccgac acgccgagat tccccgccat cctccggcct ggcgatgcga
     4621 ccctggcgct ccccgcgtgc gccaagttcc ggaacgactg agcgtccgag cgtctggtcc
     4681 cttccctcca gcaggtgcgg caggcggtgt tccaggtcgg aaaggagggg ctggtctgcc
     4741 cgggcgcggg tgatctcgcc catatcgagg gcggcggtca gcaccgccgg ctcgaagacc
     4801 ggaccctgga ggagcgtctc gccgcgggga ctgaggagca ccgacccgcc cgcgaacccc
     4861 ttcccccctt cgaatcccac gagctg
//
LOCUS       DASUAP010000314         4886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_868848, whole genome shotgun sequence.
ACCESSION   DASUAP010000314 DASUAP010000000
VERSION     DASUAP010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868848"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1274
                     /locus_tag="VFU45_08480"
     CDS             243..1274
                     /locus_tag="VFU45_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5359142.1"
                     /translation="MTPHSQPTTDITIIGGGPTGLFGAFYAGMRGASCRIIDALPELG
                     GQLMALYPEKHIYDVAGFTKVLAKDLVAAMVHQGLQFGATAHLGQTVTGLRREVRDGE
                     ELFILDTTAGSYPTRTIVLAAGIGAFAPRTLPLEGSERWEGKGLRYHIREPAEFAGQR
                     VLLVGGGDSAFDWALHLHGVARSVTLIHRRDQFRAHQATIDQVQKLAEQGKLDLRTFT
                     EVAAIHGTARIDGATLRHAKTGAEERIAVDAIVPLLGFHSDLGAINAWGLDTDKADIK
                     VNQVMETNVPGIYAAGDVVSYPGKLKLIATGVAEACTAVNNAVHRINPKAKVNPGHSS
                     DLKIFEEKA"
     gene            complement(1479..3008)
                     /gene="dacB"
                     /locus_tag="VFU45_08485"
     CDS             complement(1479..3008)
                     /gene="dacB"
                     /locus_tag="VFU45_08485"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEU5359143.1"
                     /translation="MNRHGDAGAQGRRNAGIFLVALALAVVLPLRLSAQSLQKRLDRR
                     LAATPWDRSLWGIAVIDDQGKLRYGVNQDRLFIPASNTKLLVTSVASALLPPDWTVKT
                     SVYAAGPVTDGVVEGDLVLYGRGDPTLGRSKRCYSVDTTLAGVCDTDPYAELRQLVGG
                     LRARGITAVAGDLVGDGSYFETETVNPWWEGYDLNWWYAAPVTGLAFNDNSVDIRWGP
                     DTLRGGPALLSISPLVGDLTLENRTRTRADSSPTTIDFFRTPGTLSLWAEGDVNRASH
                     GGLESFAMPDPNLFTARAFRQVLADSGISVLGTTRSTTDSLEYLAARTSPALAEAAGR
                     PLKDWIFPILNTSQNWYAEMLLKQLGKVFGKAGSWKEGLAVERRFLVDSMHVDSTQFA
                     TEDGSGLAASDLVSPLAFTQILRYTRSHRGWATFEPGLPLAGAPGSLKDRFHDTPLAG
                     RVRAKTGSIGRVNTLSGFIEFPDGKVWTVSVLANHHAQRGRDALAEIDSLVVDIGRSR
                     K"
     gene            complement(3001..3921)
                     /locus_tag="VFU45_08490"
     CDS             complement(3001..3921)
                     /locus_tag="VFU45_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5359144.1"
                     /translation="MIRLLLRRLLALAPTLLGVLVLAFLLLQVAPGDPVAAIVGERAD
                     PATVARLRAELRLDEPLPRRFLDYVVGIVHGDLGTSFITRRPIARDLIERFPATLRLA
                     LAAMLFAAVTGMAFGVVGAVRPGGWWDRAMTIGAYLGVSFPVYWVGLLLILVFALTLR
                     WLPPSGSDGLAYLILPALTLGMRSVAIIARMTRTALAEALTGDFVRTARAKGLPERRV
                     VLVHALRNALLPVITVLGLDFGSYLTGSILTETIFSWPGVGRYVLDAITKRDLPAVQG
                     SILFLCVTFVFVNLITDLCYAKADPRVAFE"
     gene            complement(3918..>4886)
                     /locus_tag="VFU45_08495"
     CDS             complement(3918..>4886)
                     /locus_tag="VFU45_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5359145.1"
                     /translation="SGPWRFVSWRHDDQIVVAKNPDYWGGPPKADSLRIRIIPEAFTQ
                     AAEYESGRLSVVEVPFGETARWEQDHAAELDRRPALRDLYVAINTRRGPLADVRVRQA
                     LNYATDVSALLDQIMGGRGARSAGALPPGIDGYDSTRAPYPHDVAKARALLAAAGHPD
                     GISLQLWRTNRDEYGRLAQAIQQQLAEAGIKVEIVERDASTARAASTHGDADLFLTDW
                     YADYPDAEDFNFPLFHSANAGAGGNRAFLTDSALDRMIVRARETPDSAERVRLDRQID
                     ARVFDLAPWIFLWAPVDLWARQPYVHGWRVPAIFTGQRWTEAVVAR"
BASE COUNT          781 a         1648 c         1767 g          690 t
ORIGIN      
        1 cgaggcggcg tgcgtaggga tcgcggaaga gcgcgtcggg gcgctccgac tccatcgccc
       61 ggtagtacgc cacccagcgg gccgtatcgg agatgtgctc gatcatcggg ggaatgtgcc
      121 cggcgccggg cggtcaagga agaggacgtc tatattcggg cggtccggcg gcgggagagc
      181 aacgcacgtc tcacgtctca cgtctcacga cccacgacta acgaccaacg acgatcgacg
      241 ccttgacccc acactcgcaa cccacgaccg acatcaccat catcggcggc ggccccaccg
      301 gcctcttcgg cgcgttctac gccgggatgc gcggagcctc ctgccgcatc atcgacgcgc
      361 tgccggaact cggcggccag ctgatggcgc tctatcccga gaagcatatc tacgacgtgg
      421 ccggcttcac caaggtgctg gccaaggatc tcgtcgccgc gatggtgcac caggggctcc
      481 agttcggcgc gaccgcccac ctgggccaga cggtcaccgg actccggcgg gaggtacgtg
      541 acggcgagga gctcttcatc ctcgacacga cggcgggcag ctatccgacg cggaccatcg
      601 tgctcgcggc ggggatcggc gccttcgcgc cccgcacgct cccgctcgag ggatcggagc
      661 ggtgggaagg gaaggggctc cgctaccata tccgggagcc ggcggagttc gcggggcagc
      721 gcgtgctgct ggtgggtggt ggcgattcgg cgttcgactg ggcgctccac ctccacggcg
      781 tggcccgcag cgtgacgctg atccaccgcc gggaccagtt ccgcgcccac caggccacga
      841 tcgaccaggt gcagaagctc gccgagcagg ggaagctcga cctccgcacg ttcaccgagg
      901 ttgccgccat ccacgggacg gcgcgcatcg acggcgccac cctgcggcac gccaagaccg
      961 gcgcggagga gcgcattgcc gtcgacgcga tcgtcccgct cctcgggttc cactccgacc
     1021 tgggcgccat caacgcctgg ggcctcgaca ccgacaaggc cgacatcaag gtgaaccagg
     1081 tgatggagac gaacgtgccg gggatctacg ccgccggtga cgtcgtgtcc taccccggaa
     1141 agctcaagct cattgcgacc ggcgtggccg aggcgtgcac cgccgtcaac aacgccgtcc
     1201 accggatcaa ccccaaggcc aaggtgaacc cgggccactc ctccgacctc aagatcttcg
     1261 aggagaaggc gtgagcggcg acggggcagt gaagagtgaa aagtgaagag cgaaaagggg
     1321 gcgccgatga tgatcgggcc cccttgtgca ttggcaggtt gtcagcatac tcggtccgtc
     1381 caacgtccaa cgtccaacgt ccaacgtcca acgtctaacg tctaacgtcc cccatttcac
     1441 ttttcactct tcacttttca ctcttcacac ttcacttctc acttcctgct ccgcccgatg
     1501 tccacgacga gcgaatcgat ctccgccagc gcatctcgcc cgcgctgggc gtggtggttg
     1561 gccaggacgg acacggtcca gaccttcccg tcggggaact cgatgaatcc cgacagcgtg
     1621 ttgacgcgcc cgatgctccc ggtcttggcg cgcacccgcc cggcgagcgg ggtgtcgtgg
     1681 aaccggtcct tgagcgaccc cggcgccccc gcgagcggga ggccgggctc gaaggtggcc
     1741 cacccgcggt ggctccgggt gtagcggagg atctgcgtga acgcgagcgg gctcacgagg
     1801 tccgacgccg cgagcccgga tccgtcctcg gtggcgaact gggtcgagtc gacgtgcatc
     1861 gagtcgacga ggaaccggcg ttccaccgcg agcccctcct tccagctccc cgccttgccg
     1921 aagaccttcc ccagctgctt gagcagcatc tcggcgtacc agttctggct ggtgttgagg
     1981 atcgggaaga tccagtcctt gagcggccgc ccggccgctt ccgccagcgc cggcgacgtc
     2041 cgcgcggcga ggtactcgag gctgtccgtg gtggagcgcg tggtgccgag gacgcttatc
     2101 cctgagtcgg cgaggacctg ccggaaggcg cgggccgtga agaggttggg gtcgggcatc
     2161 gcgaaggact cgagcccgcc gtggctcgcg cggttcacgt ccccttcggc ccagagcgag
     2221 agggtgccgg gcgtccggaa gaagtcgatc gtggtgggcg agctgtcggc ccgggtgcgg
     2281 gtgcggttct cgagcgtcag gtcgcccacg aggggcgaga tgctgaggag cgccgggccg
     2341 ccgcgcaggg tgtccggccc ccagcggatg tccacgctgt tgtcgttgaa cgcgagcccc
     2401 gtcaccggtg ccgcgtacca ccagttgagg tcgtacccct cccaccacgg gttcacggtc
     2461 tcggtctcga agtagctccc atcgcccacg aggtcgcccg ccacggccgt gatgccgcgc
     2521 gccctgagcc ctcccacgag ctggcgcagc tcggcgtacg ggtcggtgtc acagacgccg
     2581 gcgagcgtgg tatcgacgct gtagcagcgc ttggagcggc cgagcgtcgg gtcgccccgc
     2641 ccgtagagga cgaggtcccc ttcgacgacg ccgtcggtga cagggccggc ggcatagacg
     2701 ctggtcttga cggtccagtc gggcggcagg agcgccgacg cgaccgaggt gaccaggagc
     2761 ttcgtgttgc tcgccgggat gaagagccgg tcctggttga cgccgtagcg gagcttcccc
     2821 tggtcgtcga tgacggcgat cccccagagg ctccggtccc acggtgtcgc cgcgagccgg
     2881 cggtcgaggc gcttctggag cgactgggcg gagagacgga ggggcaggac gacggccaaa
     2941 gcgagggcga cgaggaagat gccggcgttc ctgcgcccct gcgcccctgc gtccccgtgc
     3001 ctattcaaac gccacccgtg ggtcggcttt cgcatagcag agatccgtaa tgaggttcac
     3061 gaacacgaac gtcacgcaca ggaagaggat gctcccctgg accgccggca gatcgcgctt
     3121 ggtgatcgcg tcgagcacgt accgcccaac cccgggccag gagaagatcg tctcggtgag
     3181 gatcgatccg gtcaggtagc tgccgaagtc gaggccgagg acggtgatga cggggaggag
     3241 ggcgttgcgc agtgcgtgga ccagcaccac gcgccgctcg gggagcccct tggcgcgcgc
     3301 ggtgcggacg aagtcgccgg tcagggcctc tgccagcgcg gtccgggtca tccgggcgat
     3361 gatggccacc gagcgcatcc cgagcgtcag cgccggcagg atgaggtacg ccaggccgtc
     3421 ggaccccgat ggcgggagcc accggagcgt cagggcgaac acgaggatga gcagcaggcc
     3481 cacccagtac accgggaacg acacgcccag gtaggcgccg atggtcatcg cccggtccca
     3541 ccaccccccg ggccgcaccg cgccgacgac gccgaacgcc atcccggtga cggccgcgaa
     3601 gagcatcgcc gcgagcgcca gccggagcgt ggccgggaag cgctcgatga ggtcccgcgc
     3661 gatgggccgc cgggtgatga acgaggtgcc gaggtcgccg tggacgatgc cgacgacgta
     3721 gtcgagaaag cggcggggaa ggggttcgtc gagccggagc tccgcgcgga gccgggccac
     3781 ggtggcgggg tcggcgcgtt ccccgacgat cgcggcgacg ggatcgcccg gggccacctg
     3841 caggaggagg aaggcgagca ccagcacgcc gaggagggtc ggcgccagcg ccagcagccg
     3901 gcggaggagg agtcggatca ccgcgcgacc accgcctcgg tccagcgctg tccggtgaag
     3961 atcgccggca cccgccagcc gtggacgtag ggctggcggg cccagaggtc caccggcgcc
     4021 cagaggaaga tccacggcgc caggtcgaag acccgcgcgt cgatctggcg gtcgagccgc
     4081 acccgctccg cggagtcggg ggtctctcgg gcgcggacga tcatccggtc gagggcgctg
     4141 tcggtcagga aggcgcggtt gccgcccgct cccgcgttgg cggagtggaa cagcggaaag
     4201 ttgaagtcct cggcgtcggg ataatcggcg taccagtcgg tgaggaagag atcggcatcg
     4261 ccgtgcgtgg aggcggcgcg tgccgtcgac gcatcgcgct cgacgatctc caccttgatg
     4321 cccgcttcgg cgagctgctg ctggatggcc tgggcgaggc gaccgtattc gtcgcggttg
     4381 gtccgccaga gctggagcga gatcccgtcg gggtgcccgg ccgcggccaa gagcgcccgg
     4441 gccttggcca catcgtgcgg gtagggggcc cgggtcgagt cgtacccgtc gatgccggga
     4501 gggagggcgc cggcggagcg ggcgccacgc ccgcccatga tctggtcgag gagggctgag
     4561 acgtcggtcg catagttgag ggcctggcgc acccgtacgt cggcaagcgg gccgcgacgg
     4621 gtgttgatcg ccacgtacag gtcccggagc gccgggcgcc ggtcgagctc ggcggcgtgg
     4681 tcctgctccc agcgcgccgt ctcgccgaag ggcacctcga ccaccgatag gcggcccgac
     4741 tcgtactcgg ccgcctgggt gaacgcctcg ggaatgatcc ggatgcgcag tgagtcggcc
     4801 ttcggcgggc cgccccagta gtccgggttc ttcgcgacca cgatctggtc gtcgtggcgc
     4861 cacgagacga agcgccacgg gccgct
//
LOCUS       DASUAP010000315        11284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_64913, whole genome shotgun sequence.
ACCESSION   DASUAP010000315 DASUAP010000000
VERSION     DASUAP010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11284
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64913"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFU45_08500"
     CDS             complement(<1..267)
                     /locus_tag="VFU45_08500"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5359146.1"
                     /translation="MFAIEPEACVGCLACLRVCPADAVAVDEAVVRIVDASCVRCGLC
                     LPACPHDAITARGELARAVAAAVRGDGVLILSPEAVTHFFPATPE"
     gene            complement(376..1884)
                     /locus_tag="VFU45_08505"
     CDS             complement(376..1884)
                     /locus_tag="VFU45_08505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5359147.1"
                     /translation="MPRILLTMDDLETAVRSNAALEAAGHQTAMVAAVDDVRAAVRRE
                     HPDLVVLTGGVREPRALQLAQLARESEISTLALLESTDTERNDRAERLDITEALVKPV
                     TADDVVATVARLLERRDLQRRTGIFGESAPIQEVLVRIEQLAPVSSTVLIQGESGTGK
                     ELVAKAIHDLSPRRGKPFIAVNCSALPENLLESELFGHEKGAFTGAAERRLGRFELAD
                     EGTIFLDEIGEIPESTQVKLLRVLESRSFFRVGGTQPIKVNVRVVAATNRSLRDEVSL
                     GAFRDDLYYRLNVLNIYLPPLRERREDIPLLVRRFIREFAKAHDRKFQGITAEALQKL
                     VDAPWPGNVRQLRNLIESMVVLAPGTEIRANDIPADILEGAGRLLPVRVGTLGAGGRE
                     VGSQELEFILRSLMDLRLQVEELRRRLDEGPQRVHVIEVGDGQPVADLVEHAAEAPPE
                     ILYRPGMTMAEVERAAIEAALREHRGNRRKAAEILGIGERTLYRKLKAYGLG"
     gene            complement(1884..2369)
                     /locus_tag="VFU45_08510"
     CDS             complement(1884..2369)
                     /locus_tag="VFU45_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEU5359148.1"
                     /translation="MTLRIAILTISDAGSRGERADTSGDAIAAWAAARGYVLAERALV
                     ADDPAAIAERLSAWADRDAAELILTTGGTGLAPRDTTPEATERILERRAPGIAELLRA
                     AAVPANPRAALSRGVAGVRGRALIVNLPGSTAGVRDGLAALEPVVDHAVDLVTGRRTA
                     H"
     gene            complement(2459..3565)
                     /gene="gcvT"
                     /locus_tag="VFU45_08515"
     CDS             complement(2459..3565)
                     /gene="gcvT"
                     /locus_tag="VFU45_08515"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU5359149.1"
                     /translation="MSDALRHTPLHAQHVKLGAKMVPFAGFEMPVQYAGGITAEHKAV
                     RENVGLFDVSHMGEFEVSGVDARAWVNKITCNDAGTLQPGEVQYSALLTEAGTFVDDC
                     TIYRFEDKVMLVVNAANIAKDWEHIVALKGGANIRLKDISHEVGLLALQGPNAEPVLQ
                     KFTDIKLNALKYYHFATGKVAGKQVFLSRTGYTGEDGFEIYCRASDTPAIWDALIVEG
                     KVPPIGLGARDSLRLEVAYPLYGNDIDDTTTAWEAGLGWIVKMEKGNTFVGLDGLKRQ
                     KLDGVKRKLVGFKLLEKGFPRHGYDVYYQGKKVDIVRSGTVSPSTGDAIGMTYLPADA
                     TEPGTRFEVDCRGTRVPAEVVKRPFYTKGSARKK"
     gene            complement(3672..4571)
                     /gene="argF"
                     /locus_tag="VFU45_08520"
     CDS             complement(3672..4571)
                     /gene="argF"
                     /locus_tag="VFU45_08520"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU5359150.1"
                     /translation="MPARHFLTIPDLSKAELVALLDLAQRMKTGAYRERPLAGKTLGI
                     IFAKSSTRTRVSFEVGAYQLGGHALFLSSRDIQLGRGEPVRDTARVLSRYLDGIMIRT
                     FDQAEVEELARFGSIPVINGLTDLHHPCQILADLLTIKQHLGGWEGKVVAWVGDGNNM
                     ANSWIDAAGLLGFELRLACPEGYEPNHEIFERNARLTKITITGEPEEAVSGAHVVNTD
                     VFASMGQEAEAEQRLAAFRGYQVNEALMARADRGAIFLHCLPAHRGEEVAETVLEGKQ
                     SRVWDQAENRLHAQKALMATLMG"
     gene            complement(4673..5605)
                     /locus_tag="VFU45_08525"
     CDS             complement(4673..5605)
                     /locus_tag="VFU45_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5359151.1"
                     /translation="MTRVLVAMAICCAPASVRLSAQAPRPVHDRVLQRELASAVRGFS
                     GTVGVYARQLRTGRWAGIDADTVFPTASMIKVPILIATFDAIRRGTLRYDTTLTFVDS
                     LRYDTTGDLVAKLRDSARVPLDQMILMMITTSDNTAALWLQGLAGTGTAINQWLADHG
                     FVHTRVNSRTAGRHDDWLTYGWGQTTPREMTQLFTMIREHRAVSPAASEQMYRALTRI
                     YWNGDALSALPPWVQAAAKQGMVDGTRGETVLVNAPSGDYVFSIITKDQADSSWTRDN
                     AGDVLIRRVSALLWKHFEPRHPYIPAPGADSLHP"
     gene            complement(5677..7089)
                     /gene="hslU"
                     /locus_tag="VFU45_08530"
     CDS             complement(5677..7089)
                     /gene="hslU"
                     /locus_tag="VFU45_08530"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEU5359152.1"
                     /translation="MTDPAAPDPTAPEPLPWLEEMPPRQIVGELDRYIVGQDAAKRAV
                     AIAIRNRWRRMQAPDPLREEITPYNIILIGPTGVGKTEVARRLARLAGAPFVKVEASK
                     FTEVGYVGRDAESMVRDLVDAAITMVRGEREDDIFPQAERRADDRLLELLLPPPPPPP
                     GPEGGPAAPPAAEQERRARALDKLREQLKDGKLDEREVEIELAAQSYPMPEWMQPPQG
                     PEGADFNVGEWLQEMLPKRKKRRTVRVVDARRILVDEELRKMVDMDDVVNEALDRVEN
                     HGIIFIDEIDKIAGERSQTGPDVSREGVQRDLLPIVEGSTVQTRYGYVRTDHILFIAA
                     GAFHVSKPSDLIPELQGRFPIRVELQPLTEADFVRIMTEPENALTKQYQALVAAEGAS
                     LEFTADGIAEIARLASMANDRMENIGARRLHTVLSTLMEQTLFDLPDGSTRRIVVDRE
                     AVTAKLAAIIGNDDLRRYIL"
     gene            complement(7177..7722)
                     /gene="hslV"
                     /locus_tag="VFU45_08535"
     CDS             complement(7177..7722)
                     /gene="hslV"
                     /locus_tag="VFU45_08535"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEU5359153.1"
                     /translation="MRHEMHGTTILAVRKGGRLALGGDGQVTVGDTVLKANANKVRSL
                     KGGKILAGFAGSVADAITLFEKFEEKLDRYPGNLPRAAVELAKDWRSDRVLRRLEALL
                     IVADLEHVFLLSGSGELIEADEGVLAVGSGGSYALAAARALYPDPSIDAADVVRRALE
                     IAADICVFTNRNLTILELGER"
     gene            complement(7747..8625)
                     /locus_tag="VFU45_08540"
                     /pseudo
     CDS             complement(7747..8625)
                     /locus_tag="VFU45_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(8739..10034)
                     /gene="trmFO"
                     /locus_tag="VFU45_08545"
     CDS             complement(8739..10034)
                     /gene="trmFO"
                     /locus_tag="VFU45_08545"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU5359154.1"
                     /translation="MRATVVGGGLAGAEAAWALAERGVAVTLCEMRPVVRTPAHQTDR
                     LAELVCSNSFKSTELTNAHGLLKAELRALGSLLLPCADLARVPAGAALAVDRDAFSAE
                     AHARLSAHPNITIERREVTALPSPAVVATGPLTSDALAEAITARLGGQALAFYDAIAP
                     IVSADSLDRERVYARSRYGKGEGDDYLNAPLDQPAYEAFIDALVAGDQFSGHDFDQVP
                     YFEGCLPVEEMARRGRETLRFGPMKPVGLADPRTGREPHAVVQLRREDRAGQMWNLVG
                     FQTRLRIPEQQRVFRTIPGLEHAEFLRYGSIHRNSYLNSPGVLGPALTARDDDRLFFA
                     GQFTGVEGYTESIGTGLLAGINLARRLRGLPVAVPPATTMLGALYRYLREADPAHFQP
                     MNANFGLLDPLEGKGRKDEKKERLVARAQADFAAWRAGL"
     gene            complement(10031..>11284)
                     /locus_tag="VFU45_08550"
     CDS             complement(10031..>11284)
                     /locus_tag="VFU45_08550"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEU5359155.1"
                     /translation="GKDARIQDAHEAIRPTDVRRRPEEVARFLEPDQARVYQLIWQRF
                     VASQMTPVVYDMTIVDFDLGPGGKYLVRATGSVMIFDGFHVLYTEGREAEEGKSMDDL
                     APIPPLAAGDRVDVREIIPHQHFTEPPPRFSEASLVKELERLGIGRPSTYSAIVSTLT
                     SREYVKVESRRFFPTQLGETVEQMMVEQFPDIFNVEFTSEMETELDRIEEGELRWDVV
                     LANFYGPFSKALEKVDIEKIVLTALKIDDPGPCPKCGGKLEFKAGRFGPYFACEHYKK
                     TCDYTKPLKRDKVPDKPTDEKCHLCGAPMVIKTGRFGEFLACTRYPECKGTRSIPLGI
                     KCPKCDVGDLAERRTKRGKSFWGCLRYPACDFSTWNKPLPETCPSCGWVGMTQKVTRA
                     DGDVRTCLKCGHKVVAAEPEEAALA"
BASE COUNT         1562 a         3887 c         4065 g         1770 t
ORIGIN      
        1 ctccggcgtg gcggggaaga aatgggtcac cgcctcgggg ctcaggatca ggaccccgtc
       61 cccccggacc gccgccgcga ccgcccgcgc cagttcgccc cgggcggtga tggcatcgtg
      121 ggggcacgcc ggcaggcaga gcccgcagcg cacgcacgac gcgtccacga tccggaccac
      181 cgcttcgtcg accgccaccg catccgccgg gcacacgcgc aggcaggcga ggcagccaac
      241 gcaggcctca ggctcgatag cgaacatcta tggggaccgg cccagggaag ggcccgaaga
      301 aggacctacc aatatcggga gtgacgcctg tcacgtcaag atgggaggca tcccaaactc
      361 tgaaagaaca ataacttagc ccagcccgta cgccttgagc ttgcggtaga gggtccgttc
      421 gccgatgccc aggatctcgg cggccttccg gcggttcccc cggtgctccc ggagcgccgc
      481 ctcgatggcc gcccgttcga cctccgccat ggtcatcccc ggacggtaga ggatctccgg
      541 cggggcctcg gcggcgtgct ccacgaggtc ggccacgggt tgcccgtcac ccacctcgat
      601 gacgtggacc cgctggggcc cctcgtcgag ccggcggcgg agctcctcca cctggagccg
      661 gaggtccatc aggctccgca ggatgaactc gagctcctgc gacccgacct cccgcccgcc
      721 ggcgcccagg gtgcccaccc ggaccggcag gagccggccc gcaccttcga ggatgtcggc
      781 cgggatgtcg ttggcgcgga tctcggtgcc cggtgccagc accaccatcg attcgatcag
      841 gttccggagc tggcgcacgt tccccggcca cggcgcatcg accagcttct gcagcgcctc
      901 ggcagtgatc ccctggaact tccggtcgtg ggccttcgcg aactcgcgga tgaaccggcg
      961 gacgaggagc gggatgtcct cccgccgttc gcgcaacggc ggcaggtaga tgttgaggac
     1021 attgagccgg tagtagaggt cgtcgcggaa ggcccccagc gacacctcgt ccctgagcga
     1081 gcggttggtg gccgccacca cccggacatt caccttgatg ggctgggtgc cgcccacccg
     1141 gaagaagctc cggctctcga gcacgcgcag gagcttgacc tgcgtgctct cggggatctc
     1201 gccgatctcg tcgaggaaga tggtgccttc gtcggccagc tcgaaccggc ccaggcggcg
     1261 ctcggcggcg ccggtaaagg cccccttctc gtgcccgaag agttcggatt cgaggaggtt
     1321 ctcggggagc gccgagcagt tgaccgcgat gaacggcttg ccgcggcggg gagagaggtc
     1381 gtggatggcc ttggccacga gctccttccc ggtgcccgac tcgccctgga tgagcaccgt
     1441 gctcgacacc ggcgccagct gctcgatccg gaccagcacc tcctggatcg gcgcgctctc
     1501 gccgaagatc ccggtgcgcc gctggaggtc tcggcgctcg aggagccgcg ccacggtggc
     1561 cacgacgtcg tcggccgtga ccggcttgac cagcgcctcg gtgatgtcga ggcgctcggc
     1621 gcggtcgttg cgctcggtgt cggtcgattc gaggagggca agggtgctga tctccgactc
     1681 acgcgccagc tgggccagct ggagggcacg cggctcgcgg acaccgccgg tgaggaccac
     1741 gaggtcgggg tgttcccggc ggaccgcggc gcgcacgtcg tccaccgcgg cgaccatcgc
     1801 cgtctggtgg ccggccgcct cgagtgcggc attggaacgc accgccgtct cgaggtcgtc
     1861 catcgtgagg aggatcctgg gcatcagtgc gccgtccgcc ggccggtgac gaggtccact
     1921 gcgtggtcca cgaccggctc gagcgccgca agcccgtcgc gcacgccggc ggtggagccg
     1981 ggaaggttga cgatgagcgc ccgaccccgc accccggcca cgccacggga gagcgccgcg
     2041 cgcgggttgg ccgggaccgc ggccgcccgc aggagctcgg cgatcccggg cgcccgtcgc
     2101 tcgaggatgc gctcggtggc ctcgggggtc gtgtcgcgcg gcgccaggcc ggtgccgccg
     2161 gtggtgagga tcagctcggc cgcgtcgcga tcggcccacg ccgaaagccg ctcggcgatt
     2221 gcggccggat cgtcggccac aagggcgcgc tccgccagca cgtagccccg tgctgccgcc
     2281 cacgcggcga tcgcgtcacc cgacgtatcg gcgcgctcgc cccggctgcc ggcgtcggag
     2341 atggtgagga tggcgatgcg gagggtcata ggatcggcgg atggtcgcac ggtcgcatca
     2401 atgccggcaa ggacgccatc cgacgatgcg gccatccgcg ggtcgtcgca ggcgacccct
     2461 acttcttcct tgccgagccc ttggtgtaga acggccgctt gaccacctcg gccgggacgc
     2521 gggtgccgcg acagtcgacc tcgaacctgg tgcccggctc cgtggcgtcg gcggggaggt
     2581 acgtcatccc gatcgcatcg ccggtggagg ggctcacggt gccgctccgg acgatgtcga
     2641 ccttcttccc ctggtagtac acgtcgtatc cgtggcgcgg gaagcccttc tccaggagct
     2701 tgaagccgac cagcttccgc ttgacgccgt cgagcttctg gcgcttcagg ccatcgaggc
     2761 cgacgaaggt gttccccttc tccatcttga cgatccagcc gagccccgcc tcccaggcgg
     2821 tggtggtgtc gtcgatgtcg ttgccgtaga gcggatacgc cacctcgagc cgcaggctgt
     2881 cgcgcgcgcc caggccgatg ggcgggacct tcccctcgac gatgagcgcg tcccagatgg
     2941 cgggcgtgtc ggaggcgcgg cagtagatct cgaagccgtc ctcgccggtg taaccggtgc
     3001 gggagaggaa cacctgcttt cccgccacct tgccggtggc aaagtgatag tacttgaggg
     3061 cgttgagctt gatgtcggtg aacttctgca ggaccggctc ggcgttcggc ccctggaggg
     3121 ccaggagccc cacttcgtgc gagatgtcct tgaggcggat gttggccccg cccttgagcg
     3181 ccacgatgtg ctcccagtcc ttggcgatgt tggcggcgtt gaccaccagc atcaccttgt
     3241 cctcgaagcg gtagatggtg cagtcgtcga cgaacgtccc cgcctcggtg agaagcgccg
     3301 agtactggac ctcgcccggc tggagggtgc cggcgtcatt gcaggtgatc ttgttgaccc
     3361 aggcgcgcgc gtcgacgccg gacacctcga actcgcccat gtgggacacg tcgaagagcc
     3421 cgacgttctc acgcacggcc ttgtgctcgg cggtgatgcc gccggcgtac tggaccggca
     3481 tctcgaagcc ggcgaacggc accatcttgg cgccgagctt gacgtgctgg gcgtggagcg
     3541 gggtatggcg gagcgcgtcg gacatgagcg tggttcctgc gttcaggtga gcggtaagcg
     3601 gtgagcggtc agcgggtccg tgagcgcggc catccggccc gctcaccttt caccgctcac
     3661 cgcttaccgg atcatcccat caacgtcgcc atcagggcct tctgggcgtg gaggcggttc
     3721 tccgcctggt cccacacccg ggactgcttc ccttcgagca ccgtctcggc cacttcctcg
     3781 ccccggtggg cggggaggca gtggaggaag atggcgccgc ggtcggcccg ggccatcagc
     3841 gcctcgttga cctggtaccc gcggaaggcg gcgagccgct gctcggcctc cgcctcctga
     3901 cccatcgagg caaagacgtc ggtgttgacc acatgtgcac cgctcaccgc ctcctcgggc
     3961 tcgcccgtga tcgtgatctt cgtcaggcgc gcgttgcgct cgaagatctc gtggttcggc
     4021 tcgtagccct caggacaggc gagccggagc tcgaagccca ggagccccgc ggcgtcgatc
     4081 cagctgttgg ccatgttgtt cccgtcgccc acccaggcca ccaccttccc ctcccacccg
     4141 ccgaggtgct gcttgatggt gagcaggtcg gccaggatct ggcaggggtg gtgcaggtcg
     4201 gtgagcccgt tgatgaccgg gatcgagccg aaccgcgcca gctcctccac ctccgcctgg
     4261 tcgaaggtgc ggatcatgat gccgtcgagg tagcgcgaga ggacccgcgc cgtgtcgcgc
     4321 accggctcgc cccggcccag ctggatgtcg cggctggaga ggaagagcgc gtggccgccc
     4381 agctggtagg cgccgacctc gaaggacacg cgggtgcggg tcgagctctt ggcgaagatt
     4441 atcccgagcg tcttccccgc cagcggtcgc tcgcggtacg cgcccgtctt catccgctgc
     4501 gccaggtcga gcagcgccac gagctcggcc ttcgagagat cagggatcgt caggaaatgt
     4561 ctggctggca tacgctcggc tgtcaggcgg aaaccgtcat tttggcaggc ggattctacc
     4621 gggtttcggg ggtccgagca aggtcgggcg ggcgcgctgg cgccccgcca ctctatggat
     4681 gcagggagtc tgcgcccggc gccgggatgt aggggtgccg gggctcgaag tgcttccaga
     4741 ggagcgcgga gacgcggcgg atgaggacgt cgccggcgtt gtcgcgggtc cagctcgagt
     4801 cggcctggtc cttggtgatg atcgagaaga cgtagtcgcc cgagggggcg ttgacgagga
     4861 ccgtctcgcc gcgggtgccg tcgaccatcc cctgcttcgc ggccgcctgg acccaggggg
     4921 gcagggccga gagcgcatcg ccgttccagt agatgcgggt gagggcgcgg tacatctgtt
     4981 cgctcgccgc ggggctcacc gctcggtgct cgcggatcat cgtgaagagc tgggtcatct
     5041 cccgcggcgt cgtctggccc cagccgtacg tgagccagtc gtcgtgacgg cccgcggtgc
     5101 gggagttgac gcgggtgtgg acgaagccgt ggtcggccag ccactggttg atggccgtcc
     5161 ccgtgccggc caggccctgg agccagagcg cggcggtgtt gtcgctcgtg gtgatcatca
     5221 tcaggatcat ctggtcgagc ggcacccggg cgctgtcccg gagcttggcg acgagatccc
     5281 cggtcgtgtc gtagcggagc gaatcgacga aggtgagcgt cgtgtcgtac cggagggtgc
     5341 cacgacggat cgcgtcgaag gtggcgatca ggatcggcac cttgatcatg ctggcggtgg
     5401 ggaagaccgt atcggcgtcg atcccggccc agcgaccggt ccggagctgg cgggcataga
     5461 cgcccaccgt gccgctgaac ccccgcacgg cggacgcgag ctcgcgctgg aggacgcggt
     5521 cgtgcaccgg ccgtggggcc tgggcggaga ggcgcacgga cgccggggcg cagcagatcg
     5581 ccatcgcaac caacacccga gtcatcatcc gtcctccgct cctgagccgc cgagcgtccg
     5641 tgcgtctgcg cgtctgtgcg tctgagcgtc tgttccctac aggatgtacc tccgcagatc
     5701 gtcattcccg atgatggcgg cgagcttggc cgtcaccgcc tcacggtcga cgacgatccg
     5761 gcgggtggag ccgtccggca ggtcgaagag cgtctgctcc atcagcgtcg agagcacggt
     5821 gtggagccgg cgggcgccga tgttctccat ccggtcgttg gccatcgacg cgagccgggc
     5881 gatctccgcg atgccgtcgg cggtgaactc gagcgaggcg ccctccgccg cgaccagcgc
     5941 ctggtactgc ttcgtcaggg cgttctcggg ctcggtcatg atccggacga agtccgcttc
     6001 ggtgagcggc tggagctcca cccggatggg gaaccgcccc tgcaattcgg ggatcaggtc
     6061 cgacggcttg gagacgtgga acgcccccgc cgcgatgaac aggatgtggt cggtgcggac
     6121 gtacccgtag cgcgtctgca ccgtggaccc ctcgacgatc gggagcaggt cgcgctgcac
     6181 cccctcgcgg gagacgtcgg ggccggtctg ggagcgctcg ccagcgatct tgtcgatctc
     6241 gtcgatgaag atgatgccgt ggttctcgac ccggtcgagc gcctcgttca ccacgtcgtc
     6301 catgtcgacc atcttgcgca gctcttcgtc caccaggatg cggcgggcgt cgaccacgcg
     6361 cacggtgcgg cgcttcttcc gtttggggag catttcctgg agccactcgc cgacgttgaa
     6421 gtcggcgccc tcgggcccct gggggggctg catccactcc ggcatcgggt agctctgcgc
     6481 cgcgagttcg atctccacct cgcgctcatc gagcttcccg tccttgagct gttcacggag
     6541 cttgtcgagc gcgcgcgcgc gccgttcctg ctccgccgcc ggcggggcgg cggggccacc
     6601 ctcgggcccc gggggcggcg gaggcggcgg caggaggagt tcgaggagcc ggtcgtcggc
     6661 gcggcgctcc gcctggggga agatgtcgtc ctcccgctcg ccgcggacca tcgtgatcgc
     6721 agcgtcgacg aggtcgcgca ccatcgactc ggcgtcccgc ccgacgtagc ccacttcggt
     6781 gaacttcgac gcctcgacct tgacgaacgg cgcccccgcc agccgcgcca gccggcgggc
     6841 cacctcggtc ttccccacgc cggtggggcc gatgaggatg atgttgtagg gagtgatctc
     6901 ctcgcggagc gggtcgggcg cctgcatccg gcgccagcgg ttccggatgg cgatcgccac
     6961 ggcgcgcttg gccgcgtcct gcccgacgat gtaccggtcg agctcgccca cgatctggcg
     7021 gggcggcatc tcctcgagcc aggggagcgg ctcgggcgcg gtcggatcgg gggcggcggg
     7081 gtcggtcatg tgtggcggtg aacggtgaac cgtgaacggt gaacggtcaa cggggcgcct
     7141 ccatccacat cccgttcact gttgaccgtt cactgttcac cgttctccga gctccaggat
     7201 cgtcaggttg cggttggtga agacgcagat atcggccgcg atctcgagcg cccgccggac
     7261 cacgtcggcg gcgtcgatcg agggatcggg atagagcgcc cgggcggcag cgagcgcgta
     7321 gctgccgccg gagcccaccg ccagcacccc ttcgtcggcc tcgatcagct cccccgagcc
     7381 ggagagcagg aagacgtgct ccaggtcggc cacgatgagg agcgcctcga ggcgccgcag
     7441 cacccggtcg ctccgccagt ccttggcgag ctccaccgcg gcgcgcggga ggttccccgg
     7501 gtaccggtcg agcttctcct cgaacttctc gaagagggtg atggcatcgg ccaccgaccc
     7561 cgcgaacccg gccaggatct tccccccctt gagcgaccgg accttgttgg cgttggcctt
     7621 gaggacggtg tcgcccacgg tgacctggcc gtcgccgccg agcgcgagcc gcccaccctt
     7681 gcggacggcg aggatggtgg tgccgtgcat ctcgtgtctc atgggaggaa gtctaacacg
     7741 ccggccctag gcccgcgggt gcgcctggcg gtacacctgc ttgagccgct ccacgctcgt
     7801 gtgggtgtag atctgcgtgg tgctgagcga ggcgtggccc aggagctcct gcacggcgcg
     7861 gaggtcggcg ccggcatcga ggagatgggt ggcaaaggtg tggcggagcg agtggacctt
     7921 gagccccgct ccgcccgccg cctcgagcag cgtgtgcatc gcccgctgga cgctcctcgg
     7981 ggtcaggcgg cggccgcgca ggttgaggaa gagcgcccgg cggtcggccc ccggccgtgc
     8041 cgcgagcgac tcacgcacgt cgagccagcg tcggagcgcc gcgccggcgc gcttccccac
     8101 cggcacgatc cgctccttgc gccccttgcc ccgcaccttc acctggtcgc ccaggaggtc
     8161 gaggtcgccc aggtcggcac cggccagttc cgagagccgg agcccgctcg aatagaacag
     8221 ttcgagcatg gccaggtcgc gcaccgcgcc gggatcatcc tgcccggcgc gctcctccgc
     8281 cgcggcgaac accgcgtcgg tctggggccg gtcgaggtgg gccgggagcc ggcgcgggag
     8341 cttcgggccc cgggccgccc gcgccacgtt gaccttgagc ccctcctcgg cctggaggaa
     8401 ccggtagaag ctccgcaccg ccgagagcgc gcgcgccgcc gagcgcttcg actggccccg
     8461 ccgctcgagc tcgcccagga atccgcggaa cccctgccgg tcgacggtct cccagctcca
     8521 cgctccgccg tagtgacggc tgcagaactc gtggaaggcg gcgaggtcgc gcccgtacgc
     8581 caggacggtg ttcggcgact gctgcttctc atcgcggacg tggcgcagga aggccgcgac
     8641 ctcgggcaag agcccgggcg caaagtcgga gggacgcaga gacgcagcct ggtccccggc
     8701 ctctttccgc gacccggcgc cccggcgccc cggcgcgctc acagacccgc ccgccaggcc
     8761 gcgaagtccg cctgcgcccg tgccacgagc cgttccttct tctcgtcctt ccggcccttc
     8821 ccctccagcg ggtcgagaag gccgaagttg gcgttcatcg gctggaagtg cgccgggtcg
     8881 gcctcgcgga ggtagcggta gagcgcgccg agcatcgtgg tggcgggggg caccgcgacc
     8941 gggaggccgc ggagccgccg cgccaggttg atgccggcca ggaggccggt gccgatcgac
     9001 tcggtgtacc cctcgacgcc ggtgaactgc ccggcgaaga agagccggtc gtcgtcccgc
     9061 gcggtgagcg ccgggcccag cacgccgggg gagttgaggt agctgttccg gtggatgctg
     9121 ccgtagcgca ggaactcggc gtgctcgagg ccgggaatgg tgcggaacac ccgctgctgc
     9181 tcggggatcc ggagccgggt ctggaagccg accaggttcc acatctggcc cgcccggtcc
     9241 tctcgccgga gctgcaccac ggcgtgcggc tcgcgccccg tgcgcggatc ggcgagcccg
     9301 acgggcttca tcggcccgaa gcggagggtc tcgcgccccc ggcgcgccat ctcctcgacc
     9361 ggcaggcacc cctcgaagta cggcacctgg tcgaagtcgt ggccgctgaa ctggtcaccc
     9421 gccacgagcg cgtcgatgaa cgcctcgtac gccggctggt cgagcggcgc gttgaggtag
     9481 tcgtcgccct cgcccttgcc gtagcgcgag cgggcgtaca cccgctcgcg gtcgagcgaa
     9541 tcggcgctca cgatgggcgc gatggcgtcg tagaaggcga gcgcctggcc gccgaggcgg
     9601 gcggtgatgg cctctgccag ggcgtcggag gtgagggggc ccgtggccac gaccgcggga
     9661 ctcgggagcg ccgtgacctc ccgccgctcg atcgtgatgt tgggatgggc ggagagccgc
     9721 gcgtgggcct cggcggagaa cgcgtcccgg tccacggcca gcgccgcgcc ggccggcacc
     9781 cgcgccaggt cggcgcaggg caggagcagg ctcccgagcg cgcggagctc ggccttgagg
     9841 aggccgtggg cgttggtgag ttcggtggac ttgaaggagt tgctgcagac cagctcggcc
     9901 agccggtcgg tctggtgggc gggcgtgcgg accaccggcc gcatctcgca gagcgtgacc
     9961 gccacgcccc gctcggcgag ggcccaggcg gcttccgcgc ccgccagccc gccgccgacg
    10021 acggtggccc tcacgccagc gccgcctcct ccggctcggc ggccaccacc ttgtggccgc
    10081 acttgaggca ggtgcggacg tcgccgtcgg cgcgggtcac cttctgcgtc atccccaccc
    10141 agccgcagct ggggcaggtc tcggggagcg gcttgttcca ggtcgagaag tcgcacgcag
    10201 ggtagcggag gcagccccag aaggacttgc cccgcttggt gcgccgctcc gccaggtcgc
    10261 cgacatcgca cttggggcac ttgatgccga gcgggatcga ccgcgtcccc ttgcactcgg
    10321 gatagcgggt gcaggccagg aactcgccga agcgcccggt cttgatgacc atcggcgcgc
    10381 cgcagaggtg gcacttctcg tcggtcggct tgtcgggcac cttgtcgcgc ttgagcggct
    10441 tggtgtagtc gcaggtcttc ttgtagtgct cgcaggcgaa gtacggcccg aagcggcccg
    10501 ccttgaactc gagcttgccg ccgcacttgg ggcacggtcc gggatcgtcg atcttgagcg
    10561 cggtgaggac gatcttctcg atgtccacct tttcgagcgc cttggagaac ggcccgtaga
    10621 agttggcgag caccacgtcc caccggagct ccccttcctc gatccggtcg agctcggtct
    10681 ccatctcgga ggtgaactcg acgttgaaga tgtcggggaa ctgctcgacc atcatctgct
    10741 cgacggtctc gccgagctgg gtcgggaaga agcggcggct ctccaccttc acgtattcgc
    10801 gggacgtgag ggtggagacg atggcgctgt aggtcgacgg ccggccgatc ccgaggcgct
    10861 ccagctcctt gacgaggctg gcctcggaga agcgcggcgg cggctcggtg aagtgctggt
    10921 gcgggatgat ctcgcggacg tcgacccggt cgccggccgc gagcggcggg atcggcgcca
    10981 ggtcgtccat cgacttccct tcctcggcct cgcgcccctc ggtgtagagc acatggaacc
    11041 cgtcgaagat catgaccgag ccggtggcgc ggaccaggta cttcccgccg ggcccgaggt
    11101 cgaagtcgac gatcgtcatg tcgtacacca ccggcgtcat ctgcgaggcg acgaagcgct
    11161 gccagatgag ctggtacacc cgcgcctggt cgggctcgag gaacctggcc acttcctcgg
    11221 ggcggcggcg gacgtcggtg ggccggatgg cctcgtgcgc atcctggatc cgggcgtcct
    11281 tgcc
//
LOCUS       DASUAP010000316         7829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_337668, whole genome shotgun sequence.
ACCESSION   DASUAP010000316 DASUAP010000000
VERSION     DASUAP010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337668"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..707
                     /locus_tag="VFU45_08555"
     CDS             114..707
                     /locus_tag="VFU45_08555"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU5359156.1"
                     /translation="MFNGLVTAIGRVTKAVRGTDGLTLRIGAPYRGVRRGESIAVDGA
                     CLTVERVGRGWFEVHVVTTSLDRTAIGGWARDRRVNLERAVRAGDRLGGHIVQGHVDG
                     VGRVLAVADHGDARLIDIAVPAEVGAVSIPLGSITVDGVSLTVNAIPRPATVRVSLIP
                     ETLRKTTLGERVAGDPVHLEGDLIGKYVVAVMKGRGS"
     gene            746..1966
                     /locus_tag="VFU45_08560"
     CDS             746..1966
                     /locus_tag="VFU45_08560"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU5359157.1"
                     /translation="MSTDSMSFGTIEQAIADLRNGKIVIVADDNDRENEGDLVCAAEL
                     VTPETINFMATHGRGLICLALTAERCDHLGLPQMTVKNTEELATAFTVSVDAERRFGV
                     TTGISAADRAATIHVAINPATAPEDLRRPGHVFPLRARAGGVLQRVGHTEASVDLARL
                     AGLHPAGVICEILNADGTMARRGELERFAAEHRLTFITIADLVAYRLGTERLVHREAE
                     ARLPTECGEFRIIGYRNDVDDHEHVALVHGEVAGAADVLVRMHSKCLTGDVFHSARCD
                     CGPQLHAAMEHIVRAGRGVIVYLDQEGRGIGLLNKLKAYALQDQGADTVQANQRLGFA
                     PDLRNYGIGAQILRDLGLSSIRIMTNNPRKLVGLEGYGLEITERVPLQTDPTSENSAY
                     LAIKRDKLGHLLPR"
     gene            1971..2441
                     /gene="ribH"
                     /locus_tag="VFU45_08565"
     CDS             1971..2441
                     /gene="ribH"
                     /locus_tag="VFU45_08565"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU5359158.1"
                     /translation="MTEIEGRLRATGRIVLVVSRYNEVVTARLLEGARACCAAAGLAP
                     GDVDVVWVPGAFELGVAATVAARSGRYSCVVALGAVIRGETPHFDFVAGEASRSLAAA
                     ALETGRPVGFGLLTTDDLDQALARAGGAAGNKGYEAAEAAIRAADVLRQLGRGS"
     gene            2438..2860
                     /gene="nusB"
                     /locus_tag="VFU45_08570"
     CDS             2438..2860
                     /gene="nusB"
                     /locus_tag="VFU45_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU5359159.1"
                     /translation="MRRPESKHRARALQLLYAWEVGGGGGAADVGSGMVRLAEHPAEM
                     ADQLIAAEAMAGAVMADLAALDREASAAAENWRWERIGVIERNILRLGVLELRDPAVP
                     PKVAIDEALHLAHLFGGAKSAAFINGVLDRVARTLGRL"
     gene            2857..3978
                     /locus_tag="VFU45_08575"
     CDS             2857..3978
                     /locus_tag="VFU45_08575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5359160.1"
                     /translation="MNILLVNWNDPENPHAGGAEVHLFEIFSRLVRRGHRVRLVCSGW
                     PGAAPRATIDGIEVLRTGGRHTFALRGRGAVRRALAAEPVDVLVEDINKVPLYTAGLT
                     RAAHCALVPHLFGATAFHEADPASAAIVWLSERPIPWAYRRSGWHAISESTRDDLVAR
                     GVPRERIEVIHPGVDAVRFCPDPAVRRTPAPSFLFLGRLKRYKGVETAIRALALARAR
                     RSDLRLDVVGSGDDGARLESLAAGSGLGDAVRFHGRVTEAEKLRFLRSAWANVFPSPK
                     EGWGITNVEAAACGTPSLASDAPGLRDSVRHGETGFLVPHGDAPALAERYLALADDPA
                     LVERLGRQARAFAETLTWESAAQRTEAHLSSLIAAARRG"
     gene            3994..4302
                     /gene="raiA"
                     /locus_tag="VFU45_08580"
     CDS             3994..4302
                     /gene="raiA"
                     /locus_tag="VFU45_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU5359161.1"
                     /translation="MHTTITARHCQITDALRERAQEVCDRLAKHAGRPVDGSVIFDVE
                     RLEQTVELKIHVSQGDVYVATGAGPDHRTALDRAEERLRRQLERHHIQPARTAGTAEA
                     "
     gene            4299..5264
                     /gene="hprK"
                     /locus_tag="VFU45_08585"
     CDS             4299..5264
                     /gene="hprK"
                     /locus_tag="VFU45_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEU5359162.1"
                     /translation="MTLRIKDLLSRKGDPLQLESLTGENGLERFVPDEEVGSPGLVLA
                     GYTDRFQPRRLHVLGETEVTYLQSLEPEQRKKVILAFFGFEPPCVFITKGQEAPPELL
                     EAARATGTPVLRTRLKTGEFYRRIKPVLEAAFAQRTSLHGSLADVYGVGLLFTGRSGI
                     GKSECVLDLVERGHRLVADDVVLVTRRGHDVLIGQGHELAGHHMEIRGVGLVDIPALF
                     GIRAVRMQKRIEVVVHLEDWEKAQAADRTGLQREETLILDVPVPKVIVPLNPGKNITV
                     IAEVVAMMHLLRFSGIDVAAKFNERLIRKMKEAQGVREYLQGDYE"
     gene            5257..5631
                     /locus_tag="VFU45_08590"
     CDS             5257..5631
                     /locus_tag="VFU45_08590"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359163.1"
                     /translation="MSEPLRGIVAAHGALADALVKAAESISGVSGALRPVSNVDCDRG
                     RLEARLAEAIGPGPAVIFVDLPSGSCHFAALHRYHEDPRVRVITGVNLAMLVDFVFHR
                     ELPLDEAAAKAMAAGTRAIKVP"
     gene            5631..6104
                     /locus_tag="VFU45_08595"
     CDS             5631..6104
                     /locus_tag="VFU45_08595"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5359164.1"
                     /translation="MPIALFRVDDRLVHGQVVIGWGRPLGADFVVVVDDALAASAWEQ
                     DLYRMGVPEGLDLAFCTVAEAVRRAPEWAARPGAGILLTGSIAAMRALREAGWAIPRV
                     NLGGIHAGPGRSERLPYLFLSHADLADLRALAASGVAVTAQDVPTAAPVALGSLQ"
     gene            6101..6769
                     /locus_tag="VFU45_08600"
     CDS             6101..6769
                     /locus_tag="VFU45_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU5359165.1"
                     /translation="MSTAALAGLLAVGTAAGLDLASGPQVLLARPLVAGTLAGLCLGD
                     PAVGLLVGSALELFALEVLPIGASRYPDHGPGAVGAVVAVSLAGAGELWRGVALGLVL
                     ALVGSWSLHRLRRANARSVAGASAALQALVPGIIGRLHWQAFRRDALRSAALTALGLV
                     AGWCVGRAPAVPGGWSPWLNATGMGAGAAAALNGALRNAGRGPRVAALGTGLLAGALW
                     LVLA"
     gene            6766..7497
                     /locus_tag="VFU45_08605"
     CDS             6766..7497
                     /locus_tag="VFU45_08605"
                     /inference="COORDINATES: protein motif:HMM:NF015570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEU5359166.1"
                     /translation="MRSDLRAFGRLLTVQASWNYERMAGIGLGYATEPLLTDLAQSDP
                     SRVGDATRRATGFFNSHPYLAGVAAGAEARAEYDGVDGGQVERLRAALSSPLGALGDQ
                     LFWAGVWPALLGVALIAVALGAGWAVPVAAVAVMLVVRLRMTRWSLRTGLRSGMQVGA
                     ALQHTRLRSLIPGFGLAAAFAVGAAVPLVAREAAGVRGWPAAVAAAVAVAGVAVGQRR
                     LVRLTGVGFGLAAFAVVILVRLAVG"
     gene            7494..7760
                     /locus_tag="VFU45_08610"
     CDS             7494..7760
                     /locus_tag="VFU45_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEU5359167.1"
                     /translation="MITREAPIVNPLGLHARPAAQFVKVASAFGSEIEVHKDGLSVNG
                     KSIMGVMMLAAECGSTITITANGPDEAAALDALVALVARGFGES"
BASE COUNT          982 a         2810 c         2927 g         1110 t
ORIGIN      
        1 gtggcggcgt tcgccgggct cgcggcccgc cccctgtacg gcgcgccgcg ctggtcggtg
       61 agcgagcggc gggccctcgg cgaggatacc ttactggtag tggataggga gccgtgttca
      121 acgggttggt gaccgccatc gggcgggtga cgaaggcggt gcgggggacc gatgggctca
      181 cgctccggat cggcgctccg tatcgggggg tccgccgggg cgagagcatc gccgtcgacg
      241 gggcatgcct caccgtcgag cgggtggggc ggggatggtt cgaggtgcac gtcgtcacga
      301 cgagcctcga ccgcacggcg atcggtggat gggcccggga ccgccgggtc aacctcgagc
      361 gggcggtccg ggccggtgat cgcctgggcg ggcacatcgt ccaggggcac gtcgatgggg
      421 tggggcgggt gctcgccgtg gccgatcatg gcgacgcgcg gctcattgac atcgcggtgc
      481 ctgcggaggt cggggcggtg tcgatccccc tcgggtcgat cacggtggat ggtgtgagcc
      541 tcacggtgaa cgcgatcccc cggccggcga ccgtccgggt gtcgctcatc cccgagacgc
      601 tccggaagac cacgctgggc gagcgggtgg cgggcgatcc cgtccacctc gagggtgacc
      661 tcatcgggaa gtacgtggtg gcggtgatga agggtcgtgg gtcgtgagtc gtgcggtccg
      721 aatccagaat cctgagtccg aggtcgtgag caccgacagc atgtccttcg gcaccatcga
      781 acaagccatc gccgatctgc ggaacggcaa gatcgtgatc gtggcggacg acaacgaccg
      841 cgagaacgag ggcgacctcg tctgcgcggc ggagctcgtg acccccgaga ccatcaactt
      901 catggccacc cacgggcgcg ggctcatctg cctcgcgctc acggcggagc gctgcgatca
      961 tctggggctc ccccagatga cggtcaagaa caccgaggag ctggccacgg cgttcacggt
     1021 gagcgtcgac gccgaacgcc gcttcggcgt caccaccggc atctcggccg ccgaccgcgc
     1081 ggccaccatc cacgtcgcca tcaacccggc caccgcgccg gaggacctcc ggcggccggg
     1141 acacgtcttc cccctgcgcg cgcgcgccgg cggcgtgctc cagcgggtgg ggcacaccga
     1201 ggcgagcgtg gacctggccc ggctggcggg gctccacccg gccggcgtca tctgcgagat
     1261 cctgaacgcc gacggcacga tggcccggcg gggcgaactg gaacgcttcg ccgccgagca
     1321 ccggctcacg ttcatcacca tcgccgacct ggtggcctac cggctcggca ccgagcgcct
     1381 ggtgcatcgc gaggcggagg cgcggctccc gaccgagtgc ggcgagttcc ggatcatcgg
     1441 ctaccgcaac gacgtcgacg accacgagca cgtggccctg gtccacggcg aggtggccgg
     1501 ggcggccgac gtgctggtgc ggatgcactc caagtgcctc accggcgacg tgttccattc
     1561 cgcgcgctgt gactgcggcc cccagctgca cgccgccatg gagcacatcg tgcgggccgg
     1621 gcgcggcgtc atcgtgtacc tggaccagga ggggcgcggc atcggcctcc tcaacaagct
     1681 caaggcctac gccctccagg accagggcgc cgatacggtg caggccaacc agcgcctcgg
     1741 cttcgcgccg gaccttcgca actacggcat cggcgcccag atcctgcgcg acctcgggct
     1801 ctcctccatc cgcatcatga ccaacaatcc ccgcaagctc gtcgggctcg agggctatgg
     1861 gctcgagatc accgagcgcg tgccgctcca gaccgacccg acctccgaga acagcgcgta
     1921 cctcgccatc aagcgcgaca agctcggcca cctcctgccg cggtgatccc gtgaccgaga
     1981 tcgaaggccg gctccgcgcg acgggccgta tcgtgctggt cgtcagccgc tataacgagg
     2041 tggtgaccgc ccggctcctc gagggcgcgc gcgcctgctg cgcggccgcc ggcctggccc
     2101 cgggagacgt cgacgtcgtc tgggtgcccg gcgccttcga actgggcgtc gccgcgaccg
     2161 tcgcggcgcg ttcggggcgc tacagctgcg tcgtggcgct cggcgctgtg atccgcggcg
     2221 agacgcccca cttcgacttc gtggccggcg aagcgagccg atcgctcgcg gcggcggcgc
     2281 tcgagaccgg gcgccccgtg ggcttcgggc tcctcaccac cgacgacctc gaccaggcgc
     2341 tcgcccgcgc cggcggggcc gcggggaaca agggatacga agcggccgag gcggccatcc
     2401 gtgccgccga cgtgctccgg cagctggggc gcgggtcgtg aggcggccgg agagcaagca
     2461 ccgcgcccgc gccctgcaac tgctctacgc ctgggaggtg ggcgggggtg gtggcgcggc
     2521 cgacgtcggg tcgggcatgg tccggctcgc cgagcacccc gcggagatgg ccgaccagct
     2581 catcgccgcc gaggcgatgg ccggcgccgt catggccgac ctggccgcgc tcgaccgcga
     2641 ggcgtcggcg gcggcggaga actggcgctg ggagcggatc ggcgtgatcg agcgcaacat
     2701 cctccggctc ggcgtcctgg agcttcgcga cccggcggtg ccgcccaagg tggcgatcga
     2761 cgaggcgctc cacctggccc atctcttcgg cggtgccaag tccgccgcgt tcatcaacgg
     2821 cgtcctcgac cgcgtggcgc gcaccctcgg ccgcctgtga acatcctgct cgtcaactgg
     2881 aacgaccccg agaacccgca cgccgggggg gccgaggtcc acctcttcga gatcttctcc
     2941 cggctggtcc gccggggcca tcgggtgcgc ctggtctgct ccggctggcc cggcgccgcg
     3001 ccgcgcgcca cgatcgacgg catcgaggtg ctccggaccg gcggccgcca caccttcgcg
     3061 ctgcggggcc ggggcgctgt acggcgggcg ctcgccgccg agccggtgga cgtgctcgtg
     3121 gaggacatca acaaggtgcc cctctacacc gccggcctca cccgcgccgc gcactgcgcg
     3181 ctggtgcccc acctcttcgg cgccaccgcg ttccatgagg ccgacccggc gtccgccgcc
     3241 atcgtgtggc tctccgagcg gccgatcccg tgggcctatc gccgctcggg gtggcacgcg
     3301 atcagcgaga gcacccgtga cgacctcgtg gcccgcgggg tcccgcggga gcggatcgag
     3361 gtgatccacc ccggcgtcga tgcggtgcgg ttctgccccg accccgcggt gcggcgcacc
     3421 ccggcgccca gcttcctctt cctcgggcgc ctcaagcgat acaagggcgt cgagaccgcg
     3481 atccgggcgc tcgcactggc gcgggcgcgc cgttccgacc tccggctgga cgtggtaggt
     3541 tcaggggacg acggcgcccg cctcgagtcg ctggcggccg ggtcgggcct tggcgacgct
     3601 gtccgcttcc acgggcgggt caccgaggcg gagaagctcc ggttcctgcg gagcgcgtgg
     3661 gccaacgtgt tcccgtcgcc caaggaaggg tgggggatca ccaacgtgga agcggccgcc
     3721 tgcggcaccc cgtccctggc gtccgacgca cccggactgc gcgattcggt ccggcacggg
     3781 gagaccgggt tcctggtccc gcacggcgac gcccccgcgc tcgcggagcg gtacctggcc
     3841 ctcgccgacg atcctgcgct ggtcgagcgc ctcggtcggc aggcgcgggc cttcgcggag
     3901 acgctcacct gggagagcgc cgcccagcgg accgaggccc acctcagttc cctcatcgcc
     3961 gcggcccgcc gcggctgacc tccgggaggg gccatgcaca ccacgatcac cgcccgtcac
     4021 tgccagatca ccgatgcctt gcgggaacgc gcgcaggagg tctgcgaccg cctcgcgaag
     4081 catgccggcc ggccggtcga cggaagcgtg atcttcgacg tggagcggct cgagcagacc
     4141 gtcgagctca agatccacgt ctcccagggc gacgtctacg tggccaccgg tgccggcccc
     4201 gaccaccgca ccgcgctcga ccgcgcggaa gagcgtctcc ggcgccagct cgagcgccat
     4261 cacatccagc cggcccggac cgcggggacc gccgaggcgt gaccctccgc atcaaggatc
     4321 tcctgtcccg caagggcgac ccgctccagc tcgagtccct caccggcgag aacgggctgg
     4381 agcgcttcgt gcccgacgaa gaggtcggga gccccggcct ggtgctggcc ggatacaccg
     4441 accgcttcca gccgcgccgg ctccacgtcc tcggcgagac cgaggtcacc tatctccagt
     4501 cgctcgagcc ggagcagcgg aagaaggtca tcctcgcctt cttcggattc gagccgccct
     4561 gcgtgttcat cacgaagggc caggaggcgc cgcccgagct gctcgaagcg gcccgcgcca
     4621 ccggcacgcc ggtgctccgc acccggctca agaccggcga gttctaccgc cgcatcaagc
     4681 ccgtcctcga ggcggcgttc gcgcagcgga cgagcctcca cgggtcgctg gccgatgtct
     4741 acggcgtggg gctcctcttc accggccgct cgggcatcgg caagagcgag tgcgtgctcg
     4801 acctggtcga gcgcggccac cggctcgtgg ccgacgacgt ggtgctggtg acccggcgcg
     4861 ggcacgacgt gctcatcggt cagggccacg agctggccgg gcatcacatg gagatccgcg
     4921 gcgtggggct ggtcgacatc ccggcgctct tcggcatccg ggcggtccgg atgcagaagc
     4981 ggatcgaagt ggtggtgcac ctggaggact gggagaaggc ccaggccgcc gaccggaccg
     5041 ggctccagcg cgaggagacg ctcatcctcg acgtgccggt gcccaaggtc atcgtgccgc
     5101 tcaaccccgg caagaacatc accgtgatcg ccgaggtcgt ggcgatgatg cacctgctgc
     5161 ggttctccgg catcgacgtg gcggccaagt tcaacgaacg cctgatccgc aagatgaagg
     5221 aagcgcaggg tgtccgggaa tatctgcagg gcgactatga gtgagccgct ccgggggatc
     5281 gtcgccgccc atggcgcgct ggccgacgcg ctggtgaagg ccgcggagtc gatcagcggc
     5341 gtgtcgggcg cgttgcggcc ggtgtccaac gtcgactgcg accgcggccg tctcgaggcc
     5401 cgcctggccg aggcgatcgg gcccggtccg gccgtgatct tcgtcgacct gccgagcggc
     5461 tcgtgtcact ttgccgcgct gcaccggtac cacgaggacc cgcgggtccg ggtcatcacc
     5521 ggcgtcaacc tggcgatgct cgtggatttc gtcttccatc gcgagcttcc cctggacgag
     5581 gcggcggcca aggcgatggc ggccgggacc cgggccatca aggtgccctg atgccgatcg
     5641 ccctcttccg ggtggacgac cggctggtcc acggccaggt ggtcatcggc tggggccgcc
     5701 ccctcggcgc cgacttcgtc gtcgtggtgg acgatgcgct ggccgcgagc gcctgggagc
     5761 aggacctcta ccggatgggc gtgcccgagg ggctcgacct cgcgttctgc accgtggccg
     5821 aagcggtgcg ccgcgccccc gagtgggcag cccgtcccgg cgccgggatc ctcctcacgg
     5881 ggagcatcgc ggcgatgcgc gccctccgcg aagcgggctg ggccatcccc cgcgtcaacc
     5941 tgggtggcat ccacgccggc cccggtcgga gcgagcggct gccctacctc ttcctcagtc
     6001 atgccgacct ggccgacctc cgcgccctcg cggcgagcgg cgtcgcggtc accgcccagg
     6061 acgtccccac cgcggcgccc gtggcgcttg ggagcctgca gtgagcacgg ccgcgctcgc
     6121 cggtctcctg gctgtcggca ccgccgccgg actcgacctc gcctcggggc cgcaggtgct
     6181 gctggcgcgc ccgctcgtcg ccgggacgct ggccggcctc tgtctgggcg atccggccgt
     6241 cggcctgctg gtcgggagcg cgctcgagct ctttgcgctg gaggtcctgc cgatcggcgc
     6301 gtcgcgctat ccggatcacg gtcccggcgc agtgggcgcg gtggtggcgg tgtcgctggc
     6361 cggcgcgggc gagctctggc gcggagtggc gctcgggttg gtgctggcgc tcgtgggcag
     6421 ctggagcctc catcggctcc ggcgggccaa cgcacgctcg gtggcgggcg cgtcggcggc
     6481 gctgcaggcg ctggtgccgg ggatcatcgg tcggctccac tggcaggcct tccggcgcga
     6541 cgcgctccgg tcggccgcat tgacggcgct ggggctcgtg gcagggtggt gcgtcgggcg
     6601 cgcaccggcc gtcccgggcg ggtggtcgcc ctggctcaat gccaccggga tgggcgctgg
     6661 tgccgcagcc gcgctcaacg gcgcgctccg caacgccgga cgtggtccgc gggtggcggc
     6721 gctcggcacc gggctcctgg cgggcgcgct gtggctggtg ctggcgtgag gtcggacctg
     6781 cgcgccttcg gccgcctcct cacggtgcag gcgagctgga actacgagcg gatggccggc
     6841 atcgggctcg ggtacgccac ggaacccctc ctcaccgacc tggcacagag cgacccgtcg
     6901 cgcgtcggcg atgcgacccg ccgggcgacg ggcttcttca actcccaccc gtacctcgcc
     6961 ggcgtggccg caggtgccga ggcgagggcg gagtatgatg gtgtcgacgg cggccaggtc
     7021 gagcgtctgc gcgccgcgct cagcagtccg ctcggggcgc tcggcgacca gctcttctgg
     7081 gccggcgtct ggcccgcgct cctcggcgtc gcgctcatcg ccgtggcgct cggcgccggc
     7141 tgggcggtgc cggtcgccgc ggtggccgtg atgctcgtgg tgcggctccg gatgacccgg
     7201 tggtcgctcc gcaccgggct ccggtcgggg atgcaggtcg gggcggcgtt gcagcacacc
     7261 cggctgcggt cgctgatccc gggcttcggc ctcgcagccg cgttcgcggt cggcgccgcg
     7321 gtgccgctcg tggcgcggga ggcggcgggc gtgcggggct ggccggcggc ggtggccgcg
     7381 gcggtcgcgg tggccggggt cgcggtgggg cagcggcggc tggtgcgcct gaccggcgtc
     7441 gggttcgggc tcgcggcgtt cgcggtcgtc atcctcgtgc ggctggcggt cgggtgatca
     7501 cgcgcgaggc gccgatcgtg aacccgctcg ggctgcacgc ccggccggcg gcgcagttcg
     7561 tcaaggtcgc gagcgccttc gggtccgaga tcgaggtcca caaggacggc ctgagcgtca
     7621 atgggaagag catcatgggg gtcatgatgc tggcggcgga gtgcggcagc accatcacca
     7681 tcacggccaa cggcccggac gaagcggccg cgctcgacgc gctggtcgcg ctggtggccc
     7741 gtgggttcgg tgaatcgtga gcgctgagcg gatgatcaag gggatcgggg tctcgcccgg
     7801 cgtggcgtat gcgcccgcgg tggtcgttc
//
LOCUS       DASUAP010000317         6603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_896339, whole genome shotgun sequence.
ACCESSION   DASUAP010000317 DASUAP010000000
VERSION     DASUAP010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896339"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFU45_08615"
     CDS             complement(<1..282)
                     /locus_tag="VFU45_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359168.1"
                     /translation="MEIDLSTPFGVAVALLPELLLSAWALLVVLLVAWRHRTPGDARL
                     AGWLSLPGLILAAAALGWLARTGVHPAGPSLMVSLDGYRYAAGLLIVACA"
     gene            complement(287..1867)
                     /locus_tag="VFU45_08620"
     CDS             complement(287..1867)
                     /locus_tag="VFU45_08620"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU5359169.1"
                     /translation="MTEFLALIGYGNWILHALVLLPLVGIVPLLLVPAPRAKQLALVL
                     TVVEFLLSLGLWWAFDPQAGGMQFASVTPWIPRYGIAYRVGIDGLSLFLVLLTTGLMP
                     LCVLSSWHYITQRERGYYALLMALLTGILGVFISLDLFVFYVFWELMLVPMYFLIGIW
                     GGQNRLYAALKFFVYTFVGSLLMLVAILALVIHTSNVTGLITFSYDDLLAHAATLGAA
                     KPWLFLAFALAFAIKVPVFPFHTWLPDAHVEAPTAGSVLLAAVLLKMGAYGFLRFAIP
                     FFPDVALSPTVTAVVVPLAVIGIIYGALVAMVQPDFKKLVAYSSVSHLGFVMLGIWAC
                     TLQSVQGAMVIMLSHGISTGALFFLIGMLYERRHTRLIAAFGGLARVMPAFAAVLTLV
                     ALSSIGLPGTNGFVGEFLVLLGSFGRHPVATAIAATCVIFAAAYLLWAVQRIIYEKLD
                     KPENEKLPDLSLREVAVLAPLLAGILWIGLYPAPVLRRLEPAARRYLELARPEAQRAP
                     VYVVPAAPTTDPSPAGAR"
     gene            complement(1874..3886)
                     /gene="nuoL"
                     /locus_tag="VFU45_08625"
     CDS             complement(1874..3886)
                     /gene="nuoL"
                     /locus_tag="VFU45_08625"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU5359170.1"
                     /translation="MLALFQAAVPPTGAAALHWAPYVILLPFLGFLANGALALWAPKA
                     KRAVSLIGPGVLIAAFGVAVAIVAAVHSTGLIVPVVTTAWTWLPVGSLQVAFATQVDQ
                     LSAVMLLVVTGVGSLIHLYSVGYMRSDDGYARYFAYLNLFVAFMLVLVLGASMPVVFI
                     GWEGVGLCSYLLIGFWFTEKANADAGKKAFLVNRIGDVGFLLAMFITWHALGTLDFGQ
                     IAALAPSRFPINGAVVTAITLFLILGCTGKSAQIPLYTWLPDAMAGPTPVSALIHAAT
                     MVTAGVYLVARTNALFALAPFSSAVLAGIGAVTALFAATIGLRQYDIKKVLAYSTVSQ
                     LGYMFLAVGTGAYVAGIFHLATHAFFKALLFLGSGSVIHAMLHAYHSTHRHADAQDMR
                     NMGGLARHMPWTAGLMGVATLAIAGVPPFAGFFSKDEILSAAFARGAEEPVFYVFWAF
                     GLLAALCTAVYMARLMAMTFFGENRTGAREREHLHEAPWMMTGPLLVLGLLSVVGGAL
                     NLPELVGGHQALAHWLAPVVAPGAQYRGVDQLHGTTEYLLVAVAVAIAVAGLLLGWRM
                     VRAAEVLPAEAAPPERGIASLLFHKYYVDELYDALIVAPLVWFSERILWKDVDQGLID
                     GAAVNGSWKLSRALGWLGSQLQTGQVGMYIVLFLAGVLYVLHAVVR"
     gene            complement(3886..4191)
                     /gene="nuoK"
                     /locus_tag="VFU45_08630"
     CDS             complement(3886..4191)
                     /gene="nuoK"
                     /locus_tag="VFU45_08630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU5359171.1"
                     /translation="MAPSLLGPSLAVSAVLFCIGVAGVLIRRNAIMLFLCIELMLNAV
                     NLAFVALSQLRGLDGQVIVFFVMTVAAAEAAVGLAIILAVFRHSESVDLKNINLLQG"
     gene            complement(4194..4751)
                     /locus_tag="VFU45_08635"
     CDS             complement(4194..4751)
                     /locus_tag="VFU45_08635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU5359172.1"
                     /translation="MIQAVFWFFAGIAVLSALLCIVQRNVVAALLWLVATMFALAGIF
                     VLLDAQFLGVIQVLVYAGAILVLFLFVIMLLNLGHAPRDTRAWPMWLLAVMVVSALGA
                     TLLKLGGYTPGRLAEEVVGGPIAADPAQVFSSGLAAAGQVAGQGVVGAVAAPLFRQWL
                     VPFELTSVLLLAAIVGAVVLAKRKL"
     gene            complement(4756..5262)
                     /locus_tag="VFU45_08640"
     CDS             complement(4756..5262)
                     /locus_tag="VFU45_08640"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEU5359173.1"
                     /translation="MAIGVKVLPRPEREVSYVRATLKGMALTFKHMFQPSVTMQYPEQ
                     RSTDDWRISTRWRGTHRMLTDEQGRSKCVACGLCPQICPANCIKLVPGEDEQGNRYPL
                     IYEIDEFRCVFCGYCQEVCPEEAIHVGVHFENAEYSRDRFVYDLERLTSQAHTVSTLW
                     DPTDPRGE"
     gene            complement(5262..6524)
                     /gene="nuoH"
                     /locus_tag="VFU45_08645"
     CDS             complement(5262..6524)
                     /gene="nuoH"
                     /locus_tag="VFU45_08645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU5359174.1"
                     /translation="MSDLLALLTPEMKGFLLVTLGKLLVVFTVVMVGVALLTFMERKV
                     SAWMQNRLGPNRVGKGWLQPAADGLKNILKEETLPASANPVIFTLAPAMAFIPAMTLS
                     AVIPFAAPIPAFRFHLGLLGDFVYAGGIPSVVADLPVGFLFVLAISSLGVYGIALAGW
                     SSNSKYSLLGGLRSSAQMISYEVAMGLALVPVLLLSGNVSFTKIVADQQVSLLNWYVF
                     PLFLSFFVYLVSGFAEKNRLPFDLPEAESELIAGYHTEYSAMKFSMFFIAEYANMVTV
                     SAMVTTLFFGGWDIPFTHWDERAGWVQVLATGAFFFAKLMFWLFFFMWIRWTLPRFRY
                     DQLMALGWKVLLPVSLAYVMVVAVTVYVGSDVLHLTPFGLTGLLTGLSVVAGGVLFYI
                     LDRGAVIGGSRTRSGQPARVPAGAAARS"
BASE COUNT         1257 a         2187 c         2374 g          785 t
ORIGIN      
        1 cgcgcaggcc acgatgagga gcccggcggc gtagcggtat ccgtcgagcg acaccatcag
       61 cgaggggccg gcggggtgca cgccggtccg ggcgagccag cccagcgccg cggccgcgag
      121 gatgagcccg gggagcgaga gccagcccgc caggcgcgca tcgcccgggg tgcggtgccg
      181 ccaggccacc aggagcacga cgaggagcgc ccaggcgctc agcagcagct ccggcaggag
      241 cgccacggcg acgccgaacg gggtgctgag atcgatttcc atggcgttac ctggccccgg
      301 ccggtgaagg gtcggtcgtc ggcgctgccg ggacgacgta caccggcgca cgctgtgcct
      361 cgggccgggc cagctccagg taacgtcgcg ccgccggctc caggcgccgg agcaccggcg
      421 ccgggtagag gccgatccag aggatcccgg ccaggagggg cgcgaggacc gcgacctccc
      481 gcagcgacag atcggggagc ttctcattct ccggcttgtc gagcttctcg tagatgatcc
      541 gctgcaccgc ccacaggagg tacgccgcgg cgaagatgac gcaggtggcc gcgatggcgg
      601 tggcgaccgg atgccggccg aagctcccga ggagcaccag gaattcgccg acgaagccgt
      661 tggtgccggg gagcccgatc gaggagagtg ccaccagcgt gagcaccgcg gcgaacgcgg
      721 gcatgacgcg ggccagcccg ccgaaggcgg cgatcagccg ggtgtgacgc cgctcgtaca
      781 gcatgccgat gaggaagaac agcgcgccgg tggagatccc gtggctcagc atgatgacca
      841 tggcgccctg gacgctctgg agggtgcagg cccagatccc cagcatcacg aacccgaggt
      901 ggctcacgga cgagtacgcg accagcttct tgaaatcggg ctgcaccatc gccaccagcg
      961 cgccgtagat gatgccgatg accgcgagcg gcaccaccac cgccgtcacc gtgggcgaga
     1021 gcgcgacgtc gggaaagaac gggatggcga accgcaggaa gccgtacgcg cccatcttga
     1081 ggagcacggc ggccaggagg acgctgccgg ccgtcggcgc ctcgacgtgg gcatcgggga
     1141 gccaggtatg gaacgggaag accggcacct tgatggcgaa cgcgagggcg aacgcgagga
     1201 acagccacgg tttggccgcg cccagcgtgg cggcgtgcgc cagcaggtca tcgtatgaga
     1261 aggtgatcaa cccggtcacg ttgctggtgt ggatcaccag cgccaggatg gccaccagca
     1321 tgagcagcga accgacgaac gtgtagacga agaacttgag cgccgcgtag agccggttct
     1381 ggccacccca aatgccgatg aggaagtaca tcggcacgag catcagctcc cagaacacgt
     1441 agaagacgaa gaggtcgagg ctgatgaaga cgccgaggat cccggtgagg agcgccatca
     1501 ggagcgcgta atagccccgc tcccgctggg tgatgtaatg ccagctcgac agcacgcaga
     1561 gcggcatgag gccggtcgtg agcagcacca ggaagagcga gaggccgtcg atcccgaccc
     1621 ggtacgcgat cccgtagcgc gggatccacg gcgtcacgct cgcgaactgc atcccgcccg
     1681 cctgggggtc gaaggcccac cagagcccca gcgagaggag gaattccacc accgtgagca
     1741 ccagggcgag ctgcttggcg cggggcgcgg gaacgaggag gagcggcacg atccccacga
     1801 gggggagcag caccagcgcg tggaggatcc agttgccgta gccgatcagc gcgaggaatt
     1861 cggtcatgga tccttaccgc accacggcgt ggaggacgta gaggacgccg gcgaggaaca
     1921 gcacgatgta catccccacc tggcccgtct ggagctggct gccgagccat ccaagggccc
     1981 gcgagagctt ccaggagccg ttgacggccg ccccgtcgat gaggccctgg tcgacgtcct
     2041 tccacaggat gcgctcggag aaccagacca ggggcgcgac gatcagggcg tcatacaact
     2101 cgtccacgta atacttgtgg aacaggagcg acgcgatgcc ccgctccggc ggcgccgcct
     2161 cggcgggcag gacctccgcc gcgcgcacca tccgccagcc cagcaggagc ccggcgacgg
     2221 cgatggccac cgccaccgcg acaagcaggt actcggtcgt gccgtgcagc tggtccaccc
     2281 cgcgatactg cgcccccggc gccaccaccg gcgcgagcca gtgggccagg gcctgatgcc
     2341 cgccgacgag ttccggcagg ttgagggcgc cgcccaccac gctcaacagg cccagcacca
     2401 gcagcggccc ggtcatcatc cagggcgcct cgtggaggtg ctcccgctcc cgggcgccgg
     2461 tgcggttctc gccgaagaag gtcatcgcca tgagccgggc catgtagacc gccgtgcaca
     2521 gcgcggccag gagcccgaac gcccagaaga cgtagaacac cggctcctcc gcgccccgcg
     2581 cgaaggccgc cgagaggatc tcgtccttgg agaagaaccc ggcgaagggc ggcaccccgg
     2641 cgatggcgag cgtggccacc cccatcagcc ccgccgtcca cggcatgtgc cgggcgaggc
     2701 cgcccatgtt ccgcatgtcc tgcgcgtcgg cgtgccggtg ggtggagtgg taggcgtgaa
     2761 gcatcgcgtg gatcacgctg cccgagccga ggaacaggag cgccttgaag aaggcgtggg
     2821 tggccaggtg gaagatgccg gcgacgtagg cgccggtccc gacggccagg aacatgtagc
     2881 cgagctggga cacggtcgaa tacgccagca ccttcttgat gtcgtactgc cggagcccga
     2941 tcgtggcggc gaagagcgcc gtcaccgcac cgatgccggc gagcacggcg ctggagaagg
     3001 gcgcgagcgc gaagagcgcg ttggtgcggg ccacgaggta gacgccggcg gtgaccatcg
     3061 tcgcggcgtg gatgagcgcc gacaccggcg tcgggccggc catggcgtcg ggcagccagg
     3121 tgtagagggg gatctgcgcc gacttgccgg tgcacccgag gatcagaaac agggtgatcg
     3181 ccgtgaccac cgcgccgttg atcgggaacc ggctgggtgc gagtgcggcg atctggccga
     3241 agtcgagcgt gccgagcgca tgccaggtga tgaacatcgc gagcaggaag ccgacgtcgc
     3301 cgatgcggtt gacgaggaag gccttcttgc cggcgtcggc gttggccttc tcggtgaacc
     3361 agaagccgat gagcaggtag gagcagaggc ccacaccctc ccacccgatg aacaccaccg
     3421 gcatgctcgc gccgaggacg agcaccagca tgaatgcgac gaacaggttg aggtacgcga
     3481 agtaccgggc gtacccgtcg tccgaccgca tgtacccgac gctgtacagg tggatgaggc
     3541 tccccacccc ggtcacgacc aggagcatga ccgcggagag ctggtcgacc tgcgtggcga
     3601 aggccacctg gagcgagccg accgggagcc aggtccaggc cgtggtgacg accggcacga
     3661 tgagccccgt cgaatgcacg gccgcgacga tggcgaccgc cacgccgaag gccgcgatca
     3721 gcaccccggg cccgatcagg gacaccgcgc gcttggcctt cggggcccag agcgccagcg
     3781 ccccgttggc caggaaaccc aggaacggga ggaggatgac gtagggcgcc cagtggaggg
     3841 cggcggcgcc ggtcggcggg acggcggcct ggaacagtgc gagcatcagc cctgcaggag
     3901 gttgatgttc ttgaggtcga ccgattccga gtgccggaag acggcgagga tgatcgcgag
     3961 cccgacggcc gcctcggccg ccgccacggt catcacgaag aagacgatca cctgcccgtc
     4021 gaggccgcgc agctggctca gcgccacgaa ggccaggttg accgcattga gcatcagctc
     4081 gatgcacagg aagagcatga tcgcgttgcg gcggatgagc acgccggcga cgccgatgca
     4141 gaagagcacc gccgagacgg cgagcgacgg gccgaggagc gacggggcca tggtcagagc
     4201 ttccgcttgg cgaggacgac agcgcccacg atggccgcga ggagcagcac ggaggtgagc
     4261 tcgaagggga ccagccactg ccggaagagc ggcgcggcca ccgcaccgac cacgccctgc
     4321 cccgccacct ggccggccgc cgccaggccg ctgctgaaca cctgcgccgg gtcggccgcg
     4381 atggggccgc cgacgacctc ctcggcgagg cgcccggggg tgtacccgcc gagcttgagc
     4441 agcgtggcgc cgagcgcgga gaccaccatc accgccagca gccacatcgg ccaggcacgc
     4501 gtgtcccgcg gcgcgtggcc gaggttgagc agcatgatca cgaacaggaa gagcaccaga
     4561 atggcgcccg catacaccag gacctggatg acgccgagga actgcgcgtc gagcagcacg
     4621 aagatcccgg cgagggcgaa catcgtcgcc accagccaca ggagggcggc cacgacgttc
     4681 cgctgcacga tgcacaggag tgcggagagc acggcgatgc cggcgaagaa ccagaagacg
     4741 gcctggatca tgggctcact cgccccgcgg gtcggtgggg tcccagagcg tcgacaccgt
     4801 gtgcgcctgc gacgtgaggc gctcgaggtc gtacacgaac cggtcgcgcg aatactcggc
     4861 gttctcgaaa tggacgccga cgtggatggc ctcctccggg cagacctcct ggcagtaccc
     4921 gcagaagacg cagcggaact cgtcgatctc gtagatcagc gggtagcggt tgccctgctc
     4981 gtcctcgccc gggaccagct tgatgcagtt ggccgggcag atctggggac agaggccgca
     5041 ggcgacgcac ttcgagcgtc cctgctcgtc ggtgagcatc cggtgggtgc cgcgccaccg
     5101 ggtgctgatg cgccagtcgt cggtgctgcg ctgctccgga tactgcatcg tgaccgaggg
     5161 ctggaacatg tgcttgaagg tgagcgccat ccccttgagc gtggcccgca cgtagctgac
     5221 ctcccgctcg gggcgcggca gcaccttgac gccgatggcc atcaggacct cgccgcggcg
     5281 cccgcgggga cgcgcgccgg ttgacccgac cgggtccggc tgccgccgat cacggcgccg
     5341 cggtcgagga tgtagaagag cacgccgccg gccacgacgc tcaggccggt gaggagcccg
     5401 gtgaggccga acggggtgag gtgcagcacg tcactgccga catagaccgt gaccgccacc
     5461 accatcacgt aggccagcga tacggggagc agcaccttcc accccagcgc catcagctgg
     5521 tcgtagcgaa agcgcggcag ggtccagcgg atccacatga agaagaagag ccagaacatc
     5581 agcttggcga agaagaacgc acccgtcgcc aggacctgga cccacccggc ccgctcgtcc
     5641 cagtgcgtga acgggatgtc ccacccgccg aagaagagcg tcgtcaccat cgccgagacg
     5701 gtcaccatgt tggcgtactc ggcgatgaag aacatcgaga acttcatcgc ggaatactcg
     5761 gtgtgatacc ccgcgatcag ctccgattcc gcctccggca ggtcgaacgg gagccggttc
     5821 ttctccgcga accccgacac caggtacacg aagaacgaca ggaagagcgg gaagacgtac
     5881 cagttgagga gcgacacctg ctggtcggcc acgatcttgg tgaacgagac gttccccgag
     5941 aggaggagga ccggcaccag ggccaggccc atcgccacct cgtaggagat catctgcgcg
     6001 ctcgagcgca gcccgcccag gagcgagtac ttgctgttgg agctccaccc cgccagcgcg
     6061 atgccataga cgccgaggga gctgatggcg agcacgaaga ggaacccgac cggcaggtcg
     6121 gccacgacgc tggggatgcc gccggcgtac acgaagtcgc cgaggagccc gagatggaac
     6181 cggaaggcgg ggatcggcgc cgcgaagggg atgacggccg agagcgtcat cgccgggatg
     6241 aatgccatcg cgggtgcgag ggtgaagatg accgggttgg cgctcgcggg gagcgtctct
     6301 tccttgagga tgttcttgag gccgtcggcc gccggctgga gccacccctt cccgacccgg
     6361 ttggggccga gccggttctg catccaggcg gacaccttcc gctccatgaa ggtgaggagc
     6421 gccacgccca ccatgaccac cgtgaacacc accaggagct tgccgagggt gacgaggagg
     6481 aagcccttca tctccggcgt cagcagcgcg aggaggtcgc tcatcgcggc gcccccgccg
     6541 gctccgcccg gcggccggtg aagcccaggt cggcgtagcg catccccgca aaggccggca
     6601 ccg
//
LOCUS       DASUAP010000318         4732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1332490, whole genome shotgun sequence.
ACCESSION   DASUAP010000318 DASUAP010000000
VERSION     DASUAP010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332490"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFU45_08650"
     CDS             complement(<1..374)
                     /locus_tag="VFU45_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5359175.1"
                     /translation="MEAVAFVEVLDRRGQVRARQPITELPAGIGRDYHNAVILDDRWA
                     SAVHARLDRDAAGRLVVEDAGSENGLYRAGGGGRVRQVAIEGTTVVRVGRTFLRIVDP
                     SQPLPPALRYGARLPGLDGWLET"
     gene            complement(362..1651)
                     /locus_tag="VFU45_08655"
     CDS             complement(362..1651)
                     /locus_tag="VFU45_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU5359176.1"
                     /translation="MRRIALGLLLLVAAAPAAMAQDADTLPAPTGVAAQLFRRYADRV
                     AKVEVLEVGSGAKALTGSAFFVSGDGLLITNYHVVARLVRDPDSYQVQLIDHTGATRT
                     ADVLVVDVVHDLAVLETDPRPGDPYFTFAADSLDQGQRLYALGHPLELGLAIVEGTYN
                     GLLRHSLYPRIHFTGALNPGMSGGPTIDAAGRVVGINVSTAGNEVSFLVPGTRAAELV
                     AEVEAPGYRAPDDLAAEVARQLRVYQRTYLSTLFTRTVPVTQLGGATLPTDPAPYFNC
                     WADADNDPDAPYQSVVHRCSTDDEVFLAEDHWSGVIEFQHRVLTADGMPAAQFYRIYQ
                     DAFAEGTDALDDGGDDVTDFRCTTRNVRQGGARWRTAFCVRAYTQLEGLYDAVFRAAL
                     LGRADTGVITELTLSGVTYDNAVAVSRRFFGLVKWKP"
     gene            complement(1653..2408)
                     /locus_tag="VFU45_08660"
     CDS             complement(1653..2408)
                     /locus_tag="VFU45_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HEU5359177.1"
                     /translation="MHRRSLFVLAALAAAQPVLAQRPALAPRALEVKYVRDAEEYATL
                     TRMVYRMAGRSVAQQAAALPRSAVWAVVLDLDETVLDNSPYQLERAAYGLPFDGPSWD
                     AWVTRAEAELVPGVADFISGVRREGGRVAWVSDRSAAGRDPTRAVLIRNGIWRDGDLL
                     CLKDGPADSTKVLRRTEIATGHGTCAWSAGPARVLAFIGDQVKDGPGRGDFPGPGEPD
                     SAAGADSAFGTRSFIIPNPTYGSWTDKVTRRGP"
     gene            complement(2411..2710)
                     /locus_tag="VFU45_08665"
     CDS             complement(2411..2710)
                     /locus_tag="VFU45_08665"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HEU5359178.1"
                     /translation="MIRYEVMLEVEPQLAAGLADYMRGKHIPEILATGCFEDIRFERA
                     GEGRFRTSYHAATPADLERYLSRHTSGFRASLLEYFPRGVTAVRETWTELETWTR"
     gene            complement(2707..3264)
                     /locus_tag="VFU45_08670"
     CDS             complement(2707..3264)
                     /locus_tag="VFU45_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359179.1"
                     /translation="MTLRWPVAALALLAACGRHAPPARPAAPAAPIATERLRNAVYRL
                     AFDPTRTIHLVDGAATDGSELDGTMVISTRMLDSIARGTLPDGRASAAVVLMTQAGGT
                     GTFYDLALVADSAGTVSDLATVGIGDRIVLKGLRLNGDSVTVSLLTQGPKDPLCCPTL
                     PVTRHFVLDGGRLLPTDRVVPADSR"
     gene            complement(3261..3674)
                     /gene="mscL"
                     /locus_tag="VFU45_08675"
     CDS             complement(3261..3674)
                     /gene="mscL"
                     /locus_tag="VFU45_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU5359180.1"
                     /translation="MLKEFKAFALRGNVVDMAVGIIIGAAFGAIVKSLVDDVLMPPLG
                     ALIGGVDFSSLTFTLREASAGAPAVVIRYGVFLNTVVSFVIVAFAVFLLIRVMNRMLK
                     HEEAPAAPTTKDCPYCLTKVPLKATRCAACTSELK"
     gene            complement(3707..4450)
                     /locus_tag="VFU45_08680"
     CDS             complement(3707..4450)
                     /locus_tag="VFU45_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEU5359181.1"
                     /translation="MSHRFVALTALALATAPTVAAQAPTPPPTLRRLEFQGDLGYVAT
                     GGNTEVTTYNIGERVAFRPGRWVFAQTFSVIYGKSKGVVNASSISAGVRGDYGFAPKA
                     AVYTLGKFAHDSFAGIKSRWEESVGLSSKVLDSALDQFSFELGVGLIQQTPSQAGGAT
                     RDFVSARTAGVYRHNFSKTAYAQQSVEVLPNLKQGDDYRINSETDVVAPIDSHIALKS
                     GLVIHFDNLPEPGFKSTDRVFTSGLQITF"
     gene            complement(4547..>4732)
                     /locus_tag="VFU45_08685"
     CDS             complement(4547..>4732)
                     /locus_tag="VFU45_08685"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5359182.1"
                     /translation="VTYRNSAGLEFTSTVEDILLHVALHGAYHRGQVARALREGGAVP
                     NATDYIAFIRGAPAARR"
BASE COUNT          698 a         1547 c         1827 g          660 t
ORIGIN      
        1 gtctcgagcc agccgtcgag ccccggaaga cgggcgccat accggagcgc gggcggcaac
       61 ggctggctgg gatcaacgat ccggaggaag gtgcgcccca cgcgcaccac cgtcgtgccc
      121 tcgatggcca cctggcgcac ccgtccgccg ccgccggccc gatagaggcc gttctcgctc
      181 ccggcatcct cgacgacgag gcgccccgcc gcgtcgcgat cgagccgggc gtggacggcg
      241 gaggcccagc ggtcgtcgag gatcacggcg ttgtggtagt cgcggccgat gccggcgggc
      301 agctcggtga tcggctgccg cgcccggacc tgcccgcgcc ggtcgaggac ctcgacgaag
      361 gctacggctt ccacttgacc agtccgaaga accggcgact gaccgcgacg gcgttgtcgt
      421 aggtcacgcc cgagagggtg agctcggtga tgacgccggt gtcggcgcgg ccgaggaggg
      481 cggcgcggaa cacggcgtcg tagagtccct cgagctgggt ataggcccgg acgcagaacg
      541 cggtgcgcca gcgggcgccg ccctggcgga cgttccgggt ggtgcaccgg aagtcggtca
      601 cgtcatcgcc gccgtcgtcc agggcgtcgg tgccttccgc gaacgcgtcc tggtagatcc
      661 ggtagaactg ggccgccggc atcccatcgg cggtcaggac acggtgctgg aactcgatga
      721 cgccggacca gtggtcctcg gccaggaaga cctcgtcgtc ggtcgagcag cggtggacca
      781 cgctctggta cggggcgtcg gggtcgttgt cggcgtcggc ccagcagttg aagtacgggg
      841 cggggtcggt cgggagcgtg gcgccgccga gctgggtgac gggcaccgtg cgggtgaaga
      901 gcgtgctgag gtaggtccgc tggtagaccc gcagctgccg ggccacctcc gcagcgaggt
      961 cgtcgggggc gcggtaaccg ggcgcctcca cctcggcgac gagctcggcc gcgcgggtgc
     1021 cggggacgag gaagctcacc tcgttgcccg cggtcgagac gttgatgccc accacccggc
     1081 cggcggcatc gatggtggga ccgccgctca tccccgggtt gagcgcgccg gtgaagtgga
     1141 tgcgggggta gagcgagtgt cggaggaggc cgttgtaggt gccctcgacg atcgcgaggc
     1201 ccagctcgag cggatggccg agcgcataga ggcgctggcc ctggtcgagc gagtcggcgg
     1261 cgaaggtgaa gtacgggtcg cccgggcgag ggtcggtctc gagcaccgcg aggtcgtgca
     1321 ccacgtccac caccagcacg tcggccgtgc gggtggcacc ggtgtggtcg atcagctgga
     1381 cctggtagct gtcggggtcg cgcaccaggc gcgcgacgac gtggtaattg gtgatgagga
     1441 gcccgtcccc gctcacgaag aacgcggagc cggtgagcgc cttggcgccc gagcccacct
     1501 cgagcacctc caccttggcc acccggtcgg cgtagcggcg gaagagctgc gcggcgacgc
     1561 cggtgggcgc cgggagggtg tcggcgtcct gcgccatcgc cgcgggtgcg gccgcgacga
     1621 ggaggaggag tcccagcgcg atgcggcgca cttcagggtc cgcgccgggt gaccttgtcg
     1681 gtccagctcc cgtaggtcgg gttggggatg atgaacgagc gggtgccgaa ggctgagtcg
     1741 gcgccggcgg cggagtcggg ctcaccgggg ccggggaagt cgccgcgccc ggggccgtcc
     1801 ttgacctggt cgccgatgaa ggcgagcacg cgggccgggc cggcggacca ggcgcaggtc
     1861 ccgtggccgg tggcgatctc ggtgcggcgg aggaccttgg tggagtcggc ggggccgtcc
     1921 ttgaggcaga ggagatcgcc gtcgcgccag atgccgttgc ggatgagcac cgcgcgggtg
     1981 gggtcgcgcc cggcggcgga gcggtcggag acccacgcca cccgcccgcc ctcccggcgc
     2041 acgccggaga tgaagtccgc gacgcccggc acgagctccg cctccgcgcg cgtcacccac
     2101 gcgtcccatg agggtccgtc gaagggaagg ccgtaggcgg cgcgctcaag ctggtaggga
     2161 gagttgtcga ggaccgtctc gtcgaggtcg aggacgacgg cccagacggc gctgcggggg
     2221 agcgccgccg cctgctgtgc caccgaccgg cccgccatcc ggtacaccat ccgcgtcagc
     2281 gtggcgtact cctcggcatc gcggacgtac ttgacctcga gggcgcgggg cgcgagggcg
     2341 gggcgctggg cgagcaccgg ctgggcggcg gcgagcgccg ccaggacgaa cagggaacga
     2401 cgatgcatga tcacctcgtc cacgtctcca gctcggtcca cgtctcccgc accgcggtga
     2461 cgccccgggg aaagtactcg agcaggctgg cgcggaaacc gctggtgtgg cgggagaggt
     2521 agcgttcgag gtcggccggg gtcgcggcat gatagctggt gcggaagcgg ccctcgccgg
     2581 cccgctcgaa ccggatgtcc tcgaagcagc cggtggcaag gatctcgggg atgtgcttgc
     2641 cacgcatgta gtccgcgagg ccggcggcca actggggctc gacctcgagc atcacctcgt
     2701 agcggatcat cggctgtccg ccggcaccac ccggtcggtg gggaggaggc ggccgccgtc
     2761 gagcacgaag tgccgggtga ccgggagcgt ggggcagcac agcggatcct tcggcccctg
     2821 ggtgagcagg gaaacggtca ccgaatcgcc attgagccgg aggcccttga ggacgatccg
     2881 gtcgccgatc ccgacggtgg cgaggtcgct caccgtgccg gcgctgtcgg ccacgagtgc
     2941 gaggtcgtag aaggtgccgg tgccgccggc ctgtgtcatg aggaccaccg ccgcggaggc
     3001 ccggccatcg ggcagggtgc cccgcgcgat cgagtcgagc atccgggtcg agatgaccat
     3061 ggtgccgtcg agctcggaac cgtcggtcgc ggcgccgtcg acgaggtgga tcgtccgcgt
     3121 ggggtcgaag gcgaggcggt acacggcgtt ccggagccgc tcagtggcga tcggcgccgc
     3181 gggcgccgcc ggacgcgccg gcggggcgtg gcgcccacat gcggccagga gggccagtgc
     3241 cgccaccggc cagcggagcg tcacttgagc tccgaggtgc aggctgcaca gcgggtggcc
     3301 ttgagaggca ccttggtcag gcagtagggg cagtccttgg tggtcggcgc ggcgggggcc
     3361 tcctcgtgct tgagcatccg gttcatgacc cggatgagga ggaacaccgc gaacgccacg
     3421 atcacgaagc tcacgacggt gttgaggaac accccgtagc ggatgacgac cgcgggtgcg
     3481 ccggcgctcg cctcccggag cgtgaacgtc aggctggaga agtccacccc gccgatcagg
     3541 gccccgaggg gcggcatcag cacgtcgtcg acgaggctct tgacgatggc gccgaatgcc
     3601 gcgccgatga tgatgccgac cgccatgtcc accacattgc cgcgcagcgc gaacgccttg
     3661 aattccttga gcatatggcg cctccagttg gggcccggcc ggtcgctcag aacgtgatct
     3721 gcagtcccga cgtgaacacc cggtcggtgc tcttgaagcc cggctcggga aggttgtcga
     3781 agtggatcac cagcccgctc ttgagcgcga tatggctgtc gatcggcgcc accacatcgg
     3841 tctcgctgtt gatccggtag tcgtcgccct gcttcaggtt cggcagcacc tccaccgact
     3901 gctgggcgta ggcggtcttg gagaagttgt gacggtagac gccggcggtg cgggccgaga
     3961 cgaagtcgcg ggtggcgccg ccggcctggg aaggcgtctg ctggatgagg cccacaccga
     4021 gctcgaagga gaactggtcg agggccgagt cgagcacctt gctcgagaga ccgaccgact
     4081 cctcccaccg gctcttgatg ccggcgaacg agtcatgcgc gaacttgccc agggtgtaca
     4141 cggcggcctt cggggcaaag ccgtagtcgc cccggacgcc ggccgagatc gagctcgcgt
     4201 tgacgacgcc cttgctcttg ccgtagatga cgctgaacgt ctgcgcgaac acccaccgtc
     4261 cgggccggaa cgccacccgc tcgccgatgt tataggtggt cacctcggtg ttcccgccgg
     4321 tggccacgta gccgaggtcg ccctggaact ccaggcgccg gagggtgggc ggcggcgtcg
     4381 gcgcctgtgc ggcgacggtg ggcgccgtgg cgagcgccag ggcggtgagt gcgacgaacc
     4441 tgtgcgacat aggtggtcct gggtggatgc gtatggagcc gttccccgac ggcggccacc
     4501 gacgggatcg ggatggaaaa taacgccctg acccgcccct cgtcagtcag cgccgcgcgg
     4561 cgggtgcccc tcggatgaac gcgatgtaat cggtcgcatt gggaacggcc ccgccctcgc
     4621 gcagcgcccg ggccacctga ccccggtgat acgccccgtg cagcgccacg tggagcagga
     4681 tgtcctcgac ggtcgacgtg aactcgagcc cggcgctgtt gcggtaggtc ac
//
LOCUS       DASUAP010000319         8030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_474106, whole genome shotgun sequence.
ACCESSION   DASUAP010000319 DASUAP010000000
VERSION     DASUAP010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474106"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFU45_08690"
     CDS             complement(<1..316)
                     /locus_tag="VFU45_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359183.1"
                     /translation="MIAFTRPWLLPLLLLVPAWLVWRRRRRSVTGHSDVSLFRSLGGA
                     NWPVRIPVALRATALACWAVAAAGPQVGGRRVEVKKEGIAIVIAVDISSSMLAEDFSP
                     SNR"
     gene            complement(313..1128)
                     /locus_tag="VFU45_08695"
     CDS             complement(313..1128)
                     /locus_tag="VFU45_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359184.1"
                     /translation="MIALLLLLQQAAPSPAPLVGDTVWVSRTVSLGPGAGARAPAWTP
                     GGDVELLAPGTTSIHDGVVDLRFPVVVWAPGVHVIDVPGPLILRTDGSIDSVAAAPVT
                     VTIRSILPDSVKRDSVEAQPPAPVLPPVETDPRPLFIWLGGGTLLALGILLIRRRPRA
                     AEVPLPVEAPAPSPPITRWAAAGEARAACEIGALRLRVAIHRGFEPADPRLDTGALLR
                     LIEGSRPQWPVAEIRRVLGALDQVRFGASGGGGDVGPLLEDAERLAGEIGGGG"
     gene            complement(1125..2018)
                     /locus_tag="VFU45_08700"
     CDS             complement(1125..2018)
                     /locus_tag="VFU45_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5359185.1"
                     /translation="MAMNPISPSVLKQVKRLELRSRGFVTALFAGEYRSVFRGQGMEF
                     AEVRAYEEGDDFRAIDWNVSARLGAPFVKTFTEERELTVLLVVDQSGSTRFGHPVTKA
                     ALAVEVAAVLALAAAEHNDRVGALLFSDTVERVIPPAKGRRHALRVIRDLVAFAPRGR
                     RTDLAAALSYASRLLSHRSIVVVLSDFAAAGWERPLRQLAARHEVVAVTVDDPRERDL
                     PDAGWIELEDQEHGHQVLVDAGDPGVRARVALLADRRRAERARALRAAGVDQVALATD
                     RDYAGPLHRAFAMRARRLARR"
     gene            complement(2114..3100)
                     /locus_tag="VFU45_08705"
     CDS             complement(2114..3100)
                     /locus_tag="VFU45_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5359186.1"
                     /translation="MTAPRPSGAQLAAEAAPVQAAVQEVARRIVGQEAMVERLLIGLL
                     TGGHVLLEGVPGLAKTLAVRTLSEIVHASFSRIQFTPDLLPADVIGTMVFDQKTAEFT
                     VKKGPLFAHVVLADEINRAPAKVQAALLEGMQEHQVTIGGTAYPLPEPFLVLATQNPI
                     ESEGTYPLPEAQLDRFLLKVRVGYPTREQEREVLLRMSGGAPIPVSRVLDPAGILAAR
                     KAVLGVYIDQKLVDYIVDLVRATRDPGLVGLHDLKPLIAFGASPRASLALAQTARAHA
                     FLRGRNYVVPEDVKAMAPDVLRHRLVLTFEAEAEEVDSDRVIARVLEAVPVP"
     gene            complement(3266..3997)
                     /locus_tag="VFU45_08710"
     CDS             complement(3266..3997)
                     /locus_tag="VFU45_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU5359187.1"
                     /translation="MSEMRLQRALAQAGIASRRAAEELIEQGKVRVDGKVATLGQKVD
                     PARQKITVGGKAVRAVERRWLAFHKPMGVVTTAKDEEGRRTVLDYIPNPAGLTYVGRL
                     DVMTTGLLLLTTDGEAVHRLTHPKYHLPRRYTALVHGRDPKELEGLVTRRWMVDGHPV
                     VAEQVRVRPGKEGRSIVDVTLREGRNRIVRKWCEAMGCKVDRLARLAYGPIRLGELPV
                     GRSRPLTGAETKALYAAIKLDEDHE"
     gene            complement(3994..4575)
                     /gene="scpB"
                     /locus_tag="VFU45_08715"
     CDS             complement(3994..4575)
                     /gene="scpB"
                     /locus_tag="VFU45_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU5359188.1"
                     /translation="MTPLAQLIEAALWSAARPLTVEELQTLDAEATLADVRTALDQLR
                     EHYDFDQHSVELVELGGGFQVLTRPHFAQAIEAAQFAVRTPKLTGAALETLAVISYRQ
                     PVGRAEIEEIRGVNAGGVLRALQERGLIEVVGRSEALGRPLLYGTTPYFLELLGLKDL
                     ADLPRTEELSVALQPPRPPEPEADSAASVPADR"
     gene            complement(4550..5305)
                     /locus_tag="VFU45_08720"
     CDS             complement(4550..5305)
                     /locus_tag="VFU45_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEU5359189.1"
                     /translation="MTGPVSASAAPTTALGADPGFVVELEAFSGPLDLLLHLLKEEQI
                     EIGDIPIARIAEQFLRVIHALGLNQAADYLEMAGRLLRLKAQMLLPRTGDDDWEDPRH
                     ELVRRLLEYQQIKEIVRWFERAHERRSQQYARGYLPPPPELPEPPLTLDILALLQAVE
                     RVIAGIPDPVLHRVVPRALDVEGATRRVLELLDASGEVGWLEALGPRPTIVEVLSTFL
                     ALLELAKHGRVNLFQPAPFAPMVIRRDPTRPVD"
     gene            5518..5772
                     /gene="rpsT"
                     /locus_tag="VFU45_08725"
     CDS             5518..5772
                     /gene="rpsT"
                     /locus_tag="VFU45_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU5359190.1"
                     /translation="MPRIKSAKKALRKSKKATLRNRAQRSALRGAVKRVRNAATPQDA
                     QEAYRAAASLLDRAGRKNLIHRNTAARQKSRLAKVVAKKK"
     gene            complement(5850..6776)
                     /locus_tag="VFU45_08730"
     CDS             complement(5850..6776)
                     /locus_tag="VFU45_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359191.1"
                     /translation="MAKAPTAGGDPTVTRLLAERDQYAQWMNRLSVASDTVPESVREK
                     VRADYEARLVAVVEQLKQHTDQVTHQLSAQRARLDELTAKETLAQEVLSEAELRHAVG
                     EYDEAQWQKLRGETHRTLVEVREESARISEEIGRLDEVARLISAAPAAAPAAPAPANP
                     AMGTVAIPAAKRPAAKGAAAPAPAPEPVAELEVEPADEEAPKGGKGGKAPRDSARTLF
                     FPSGKGTEKGIDELDFLKSVGGEQGEKKGGSIKPAAVKEQEPVAEAPPAKAEVIKSDA
                     TAGKTLKCAECGTMNRPTEWYCERCGAELAAL"
     gene            complement(6866..>8030)
                     /locus_tag="VFU45_08735"
     CDS             complement(6866..>8030)
                     /locus_tag="VFU45_08735"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU5359192.1"
                     /translation="EVIDAKGKVVAPGLIDVHVHLREPGFEDLETIATGAAAAAAGGF
                     TAVCAMPNTDPVTDNQAAVGFIVSQARRAEMARVYPIGCITVGQKGERLAEFGELVGA
                     GAVGVSDDGKPVMSSHIMRTALEYARTFGIPVADHCEDTPLAQGGAMHEGIVSTRLGL
                     KGIPSAAEEIHVARDCILAELTGGHIHLCHMSTRGSVDLIRRAKEKGLHVTAEATPHH
                     FALTHERCEGYDTNAKMNPPLREAEDRAAIRQALKDGTIDVIASDHAPHHYDAKEREF
                     DDAPNGILGLETALGLAIRELVTPGILSLPELIFRMSTRPAQVFHLPGGTLAVGAPAD
                     LVLLDPAAKWVVRPEAFRSKSRNTPFGGETLTGRADLTLVDGRVVWRRGADAS"
BASE COUNT         1109 a         2927 c         2864 g         1130 t
ORIGIN      
        1 gccggttcga cggcgagaag tcctccgcca gcatcgagct cgagatgtcg accgcgatca
       61 cgatcgcgat cccttctttc ttcacctcga cccgccgccc ccccacctgc ggccccgccg
      121 ccgcgacggc ccagcaggcg agcgccgtcg cgcggagcgc caccgggatc cgcaccggcc
      181 agttggcgcc gcccagcgag cggaagagcg acacgtcgct gtgcccggtc accgaccggc
      241 ggcggcggcg ccagacgagc cacgcaggca ccaggagcag cagtggcagc agccatgggc
      301 gcgtgaaggc gatcatcccc cgcctccgat ctcgccggcc aggcgctcgg cgtcctccag
      361 gagggggccc acatcgcccc cgccgccgga ggcgccgaac cgcacctggt cgagggcgcc
      421 gagcacgcgg cggatctccg cgaccggcca ctgcggccgg gagccctcga tgagccggag
      481 caacgcaccc gtgtccaggc ggggatcggc cggctcgaag ccgcggtgga tcgccacccg
      541 gaggcggagc gcaccgatct cgcaggccgc gcgcgcctca ccggcggcag cccagcgcgt
      601 tatgggcggc gagggggctg gcgcctcgac ggggagcggc acctcggctg cgcggggccg
      661 gcgccggatg agcaggatgc cgagcgccag caaggtcccg ccaccgagcc agatgaagag
      721 cgggcgcggg tcggtctcca ccggcggcag caccggtgcc gggggctggg cctccacgga
      781 gtcgcgcttc acggaatcgg gaaggatgct ccggatggtg actgtgaccg gcgcggccgc
      841 caccgagtcg atggagccgt cggtccggag gatcagcggg cccggcacgt cgatcacgtg
      901 gacacccggc gcccagacca ccaccgggaa tcggaggtcg acgacgccat cgtgaatgct
      961 cgtcgtgccc ggggccagga gctcgacgtc gcccccgggc gtccaggccg gggcgcgggc
     1021 gccggcgccc ggcccgaggc tcaccgtgcg ggacacccag accgtatcgc ccaccagcgg
     1081 cgccggcgag ggggccgcct gctggaggag gagcagcagc gcgatcatcg ccgcgccagg
     1141 cggcgcgccc gcatcgcgaa cgcgcgatgg agcgggccgg cgtagtcccg atccgtcgcc
     1201 agcgcgacct ggtcgacgcc ggcggcccgg agggcgcgcg cccgctcggc gcggcgccgg
     1261 tcggcgagga gcgccacccg cgcccgcaca ccggggtcgc ccgcgtcgac gagcacctgg
     1321 tggccatgct cctggtcctc gagctcgatc caccccgcgt cgggaaggtc ccgctcgcgc
     1381 gggtcatcga ccgtcaccgc caccacctcg tggcgcgccg cgagctggcg gagcgggcgt
     1441 tcccacccgg cggcggcgaa gtcggagagc accaccacga tgctgcgatg ggagaggagc
     1501 cggctggcgt aggagagcgc cgccgcgagg tcggtccggc gcccccgggg cgcaaaggcc
     1561 acgaggtcgc ggatgacgcg cagcgcgtgg cggcgcccct tggccggcgg gatgacccgc
     1621 tcgaccgtgt cgctgaagag gagcgcgccc acgcggtcgt tgtgctccgc cgccgcgagg
     1681 gccagcaccg ccgcgacctc gactgcgagc gccgccttgg tcaccggatg gccgaagcgg
     1741 gtggaacccg actggtcgac cacgaggagc acggtgagct cgcgctcctc ggtgaacgtc
     1801 ttgacgaacg gggcgccgag ccgggcggag acgttccagt cgatggcgcg gaaatcgtcc
     1861 ccttcctcgt acgcacggac ctcggcgaat tccatcccct ggccgcggaa caccgagcgg
     1921 tattcgccgg caaagagggc cgtgacgaac ccgcggctcc ggagctcgag ccgcttgacc
     1981 tgcttgagga cggaagggga gatcggattc atcgccatgg cggatggtcc ggtcagcggt
     2041 gagcggtgag gtgtgagcgg gccgccggtg gagatcgcgc caggcggccc gctcaccgct
     2101 caccgctgac cgctcacggc accggaaccg cctccagcac ccgcgcgatc acccggtcgg
     2161 aatcgacctc ctcggcctcc gcctcgaagg tgagcaccag ccggtggcgc agcacgtcgg
     2221 gtgccatcgc cttcacgtcc tcgggcacga cgtagttccg cccgcggagg aacgcgtggg
     2281 cgcgcgccgt ttgcgccagc gcgagcgacg cccggggcga cgcgccgaac gcgatgagcg
     2341 gcttcaagtc gtggagcccg acgagccccg gatcgcgggt ggcgcggacc aggtcgacga
     2401 tgtagtccac gagcttctgg tcgatgtaga cgccgaggac cgccttccgc gccgcgagga
     2461 tcccggcggg gtcgagcacc cgcgacaccg ggatcggcgc cccgccgctc atccggagca
     2521 gcacctcgcg ctcctgctcc cgggtcggat agcccacccg taccttgagg aggaagcggt
     2581 cgagctgcgc ctcggggagc ggataggtgc cctcgctctc gatcgggttc tgggtggcga
     2641 gcaccaggaa gggctccggg agcggatagg ccgtgccgcc gatggtgacc tggtgctcct
     2701 gcatcccctc gagcagcgcc gcctggacct tggcgggcgc gcggttgatc tcgtccgcca
     2761 ggaccacgtg cgcgaacagg gggcccttct tgaccgtgaa ctccgcggtc ttctggtcga
     2821 agaccatggt gccgatcacg tcggccggga ggaggtcggg ggtgaactgg atgcgggaga
     2881 agctggcgtg gacgatctcc gacagcgtcc ggaccgcgag cgtcttggcg agcccgggca
     2941 ccccctcgag caggacgtgg ccgccggtca ggagcccgat caggaggcgc tccaccatgg
     3001 cctcctggcc gacgatccgg cgcgcgacct cctgcaccgc cgcctgcacc ggggcggctt
     3061 cagcggcgag ttgagcgcca gatggacggg gagcggtcat tgagttgtcg ttggtcgtgg
     3121 gtcattggtc gtgggtcgcg ggccgtgggt cacgggtcgc gagtcgtggg tcgtgggtcg
     3181 tgggtcgtga agtacgcacg ccggtcatga tgcggtcgat cgacggtgca cgtcatcgca
     3241 cgacccgtga cgcgcgaccc acgactcact cgtgatcctc atccagcttg atcgccgcgt
     3301 acagcgcctt ggtctccgcg cccgtcagcg gacgcgaccg ccccaccggc agttcgccga
     3361 ggcggatggg gccgtacgcc agccgggcca gccggtcgac cttgcagccc atcgcctcgc
     3421 accacttgcg gacgatccgg ttgcgcccct cgcgcagggt cacgtccacg atgctccgcc
     3481 cctccttccc cggccgcacc cgcacctgct ccgccaccac cggatggccg tcgaccatcc
     3541 accgccgggt caccaggcct tcgagctcct tcgggtcccg gccgtggacc agcgcggtgt
     3601 accgccgggg caggtggtac ttcgggtggg tcagccggtg caccgcctcg ccgtcggtgg
     3661 tgaggaggag gagccccgtg gtcatcacgt ccagacgccc tacgtacgtg agccccgccg
     3721 gattcgggat gtagtcgagg acggtgcggc gcccctcctc atccttggcg gtcgtcacga
     3781 cacccatcgg cttgtggaac gcgagccagc gacgctcgac cgcccgcacc gccttgccgc
     3841 ccacggtgat cttctggcgc gccgggtcga ccttctggcc cagggtcgcg accttcccgt
     3901 cgacccgcac cttgccctgc tcgatgagct cctccgccgc ccgccgggag gcgatccccg
     3961 cctgcgccag cgcgcgctgc agccgcatct cgctcatcgg tccgccggca cggatgccgc
     4021 cgaatcggcc tcgggctccg ggggcctggg cggctggagc gccaccgaca gctcctcggt
     4081 gcgcggcagg tcggccaggt ccttgagccc cagcagctcg aggaagtagg gcgtcgtccc
     4141 ataaaggagc ggccggccca gcgcctcgct ccgccccacc acctcgatca atccccgttc
     4201 ctggagagcg cgcagcaccc cgccggcgtt gacgccgcgg atctcctcga tctcggcacg
     4261 ccccaccggc tgccggtagg agatgaccgc cagcgtctcg agcgcggcgc cggtgagctt
     4321 gggcgtccgc accgcgaact gtgcggcctc gatcgcctgc gcgaagtggg gccgggtcag
     4381 gacctggaag ccgccgccca gctccacgag ttccaccgag tgctggtcga agtcatagtg
     4441 ctcccgcagc tggtcgaggg ccgtccgcac gtcggcgagc gtcgcctcgg cgtcgagggt
     4501 ctggagctct tcgaccgtga gggggcgcgc ggcgctccag agcgcggctt caatcaactg
     4561 ggcgagtggg gtcacggcgg atcaccatcg gcgcgaaggg cgccggctgg aagaggttga
     4621 cgcgcccgtg cttggcaagc tccagcaggg caaggaaggt cgacagcacc tcgacgatcg
     4681 tgggccgcgg ccccagcgct tcgagccacc cgacctcgcc gctggcgtcg aggagctcga
     4741 gcacccgccg ggtggcaccc tccacgtcga gcgcccgggg caccacgcgg tgaagcaccg
     4801 ggtcggggat gccggcgatg acccgttcga ccgcctgcag gagcgccagg atgtcgagcg
     4861 tgagcggcgg ctcggggagc tccgggggcg ggggcaggta tccgcgcgcg tactgctgtg
     4921 accgccgctc gtgggcccgc tcgaaccagc ggacgatctc cttgatctgc tggtactcga
     4981 ggagacgccg cacgagctcg tgccgcgggt cctcccagtc gtcgtcgccg gtgcgcggga
     5041 ggagcatctg cgccttgagc cgcagcaggc gtcccgccat ctcgaggtag tcggcggcct
     5101 ggttgaggcc gagggcgtgg atgacccgga ggaactgctc cgcgatccgc gcgatcggga
     5161 tgtccccgat ctcgatctgc tcctccttca ggaggtgcag cagcaggtcg agcgggcccg
     5221 agaacgcctc gagctccacc acgaatccgg gatccgcgcc gagcgcggtg gtcggtgccg
     5281 ccgaggcgga gaccgggccg gtcatcgcgg acggacgcgc cccgaacggg ccggcggcgg
     5341 aggacggtgg gtcagcggag attccttgtg cgggggccac tgggcggaac aataacgcgc
     5401 cgaccgcagc cgacaagcgg tcgcttgacc gaagcccctg ccgcgcctag gtttagcgcc
     5461 tgttttcgac cggccgcgat cggtggccgc tcccagtcca ggacctcgag ggattcagtg
     5521 ccaaggatca agtccgccaa gaaggcgctc cgcaagtcga agaaggccac cctccgcaac
     5581 cgggcccagc gctcggcgct tcgcggcgcc gtcaagcgcg tccgcaatgc ggccacgccg
     5641 caggacgccc aggaggcgta ccgcgccgcg gcgagtctcc tcgaccgggc cgggcggaag
     5701 aacctgatcc accgcaacac cgccgcccgg cagaagagcc ggctggccaa ggtggtcgcc
     5761 aagaagaagt gaacggtcaa cggtgaacag tgaacggggg gttccgtgca gcacggagcc
     5821 ccccgttccc tgttcaccgt tcacccgccc tacaacgccg ccagctccgc cccgcagcgc
     5881 tcgcagtacc attcggtcgg ccggttcatc gtgccgcatt ctgcgcactt gagcgtcttg
     5941 ccggcggtcg cgtccgactt gatgacttcg gccttggccg gcggcgcctc ggcgaccggc
     6001 tcctgttcct tcaccgccgc cggcttgatc gatccgccct tcttctcccc ctgctcgccg
     6061 cccacggact tcaggaaatc gagctcgtcg atccccttct cggtgccctt gccgctcggg
     6121 aagaagagtg tccgggccga atcgcgcggc gccttccctc ccttgccgcc cttcggcgcc
     6181 tcctcgtcgg ccggctccac ctcgagctcg gccaccggct ccggtgcggg cgccggggcg
     6241 gcggcgccct tggccgccgg acgcttggcg gcggggatgg ccacggtgcc catcgccggg
     6301 ttggccggcg ctggtgcggc cggtgcggcg gccggtgccg cggagatgag ccgcgccacc
     6361 tcgtccaggc ggccgatctc ctcgctgatc cgggcgctct cctcacgcac ttccaccagt
     6421 gtgcgatggg tctcgccccg gagcttctgc cactgggcct cgtcgtactc gccgacggcg
     6481 tggcggagct ctgcctcgct cagcacttcc tgggccagcg tctccttggc ggtcagctca
     6541 tcgagccgcg cccgctgggc gctgagctga tgggtgacct gatcggtgtg ctgcttcagc
     6601 tgctcgacca cggccaccag gcgcgcctca tagtcggcgc gcaccttctc ccggacactc
     6661 tcggggacgg tgtcgctcgc gacactcagc cggttcatcc attgggcgta ctgatcccgc
     6721 tcggccagga gccgagtgac ggtgggatca ccgccggcgg tgggagcctt cgccattcgg
     6781 accggtcctc tcgggggata gggcggccgt cctgggccaa cgacctacac tatatggtaa
     6841 aagaataagg ggccccggcg ccgattcaac tggcatccgc ccctcgccgc cagaccaccc
     6901 ggccgtcgac cagggtcagg tcggcccggc cggtgagggt ctccccgccg aagggggtgt
     6961 tccggctctt cgaccggaac gcctccgggc ggaccaccca tttggcggcg ggatcgagca
     7021 ggaccaggtc ggccggtgcc cccaccgcga gcgtgccgcc ggggaggtgg aacacctgag
     7081 ccgggcgggt gctcatccgg aagatcagct ccggcagcga gaggatcccc ggcgtcacca
     7141 gttcgcggat ggccaggccg agcgccgtct ccagccccag gatgccgttg ggggcgtcgt
     7201 cgaactcccg ctccttggcg tcgtagtgat gcggcgcgtg gtcgctcgcg atcacgtcga
     7261 tggtgccgtc cttgagcgcc tggcggatcg cggcgcggtc ctccgcctcc cggagcggcg
     7321 ggttcatctt ggcgttggtg tcatagcctt cgcaccgctc gtgggtcagg gcaaagtggt
     7381 gcggtgtggc ctccgcggtg acgtggagcc ccttctcctt ggcccgccgg atcaggtcca
     7441 ccgaaccacg ggtgctcatg tggcacaggt ggatgtgccc gcccgtcagc tcggccagga
     7501 tgcagtcgcg ggcgacgtgg atctcctccg ccgccgaggg gatccccttg aggcccaggc
     7561 gggtggagac gatgccctca tgcatcgccc cgccctgcgc cagcggcgtg tcctcgcagt
     7621 ggtccgccac cgggatcccg aacgtgcggg catactcgag cgccgtgcgc atgatgtggc
     7681 tcgacatcac cggcttgccg tcgtcgctca cgcccacggc gccggcaccc acgagctcgc
     7741 cgaactccgc caggcgctcc cccttctgtc ccaccgtgat gcatccgatc ggatacaccc
     7801 gcgccatctc ggcgcgccgc gcctggctca cgatgaagcc gacggcggcc tggttgtcgg
     7861 tgacgggatc ggtattgggc atggcgcaga cggcggtgaa cccgcccgcc gccgccgccg
     7921 cggcaccggt ggcgatcgtc tccaggtcct cgaagccggg ctcgcgcagg tgcacgtgca
     7981 cgtcgatgag cccgggggcc acgaccttcc ccttggcgtc gatgacctcg
//
LOCUS       DASUAP010000320         7213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_120223, whole genome shotgun sequence.
ACCESSION   DASUAP010000320 DASUAP010000000
VERSION     DASUAP010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120223"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1149)
                     /locus_tag="VFU45_08740"
     CDS             complement(49..1149)
                     /locus_tag="VFU45_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5359193.1"
                     /translation="MYSTAQFRVFGASAAERRLVRARQLARDGRSAEAEDAYQAILAE
                     DPGGRRAWGEYFEWLRSERRHDDALFLAERAATHFPGEAFALALVGAAQVELGRYRDG
                     LAALERAAEADPDLGLVWHETGYAAWRLGEASRALLAVDRAFALDPHGATLLLRGKIL
                     RDAGRYLAAEVAFTGAIEAAEFPEQRREAERQVAIVRRCAAFPGSKPATLTAARRWFA
                     ETGGAVLTSPGGDCAPADADLLRAFADLAREEGWRFTAIHATDGWPGWQALARALSLA
                     LLPVTDHAEEIPLVVTRSPVMGGLKWEMAVARLAESPLGMSFALSCPIGTSGADVVGV
                     LEGTERCHVDLAAAQEAVRHPSSRLAARKVSA"
     gene            1283..2647
                     /locus_tag="VFU45_08745"
     CDS             1283..2647
                     /locus_tag="VFU45_08745"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5359194.1"
                     /translation="MPNDIATRDQARLLGELKEFLRIPSISTLPDHAADCRRAADWVM
                     DHLRRLGCPVVELLEGKGHPTIWAEGPQVPGAPTVLLYGHYDVQPAEPLEEWVTGAFD
                     PTVRDGNLYARGAVDDKGQVFTLLKAYESVLGADRRPPLNVHFIIEGEEECGGRVIFD
                     RLKAEPWRTKADAVVVADMGYYAPGVPAVYTALRGLAYAEIQVRTAESDLHSGEYGGV
                     APNAIETTVRILAALKDASGRIKVPGIYEAVRRPSRTELAGWKKLPFDRRTYLKKQVT
                     AKALTGLQKYSIHERLWALPTLEIHGIRGGFVGEGAKTVIPCQAVAKVSLRLVPGHSS
                     ARVYRQLALAVKRVTPKWADVKVVPLHSGEPVQVEVDAPAFAVLDRAFKEVVGRPTVA
                     VRAGGSIPVVSALAQGGAPVLLTGIGLPDDRLHAPNEKVTLSQVWDGIRMFGRFFEMM
                     AEKK"
     gene            complement(2773..3312)
                     /locus_tag="VFU45_08750"
     CDS             complement(2773..3312)
                     /locus_tag="VFU45_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359195.1"
                     /translation="MADGTKRSPAQAADEKLADIQKAAGSWAREVPTALQPAVRAVCI
                     RLAEMVADQRHYLAPLPTAQESEREQMLKLLVERLTSKAAVAGIEQELKELSEMELRA
                     TGSWSLVPTAQTEWINTAIGFLDQRGKVLPKPEQPDPYWADDLVAGVVASVRAVIGVD
                     ELHARTESQFGRGKQVAEE"
     gene            complement(3390..4106)
                     /gene="lipB"
                     /locus_tag="VFU45_08755"
     CDS             complement(3390..4106)
                     /gene="lipB"
                     /locus_tag="VFU45_08755"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU5359196.1"
                     /translation="MTRALRVIDLGRRPYGDALELQRELCRRRVAGAVDDDLLLLVEH
                     DPVVTLGRGTRPESLPVPPDALRLRGVEVFEVERGGDVTVHEPGQLVGYPILDLQGHR
                     PDLHWYLRELEGVLIDALATVGIAAGREPGRTGVWVEGRKVASIGIHVKQWVTFHGFA
                     LNVTNDLRGFDAIVPCGIPGVEMTSVAREVARIGGDPTALRDRMQHAVVEALGRRFDR
                     TPVPDTLERTLGPALVSAAH"
     gene            complement(4103..4558)
                     /locus_tag="VFU45_08760"
     CDS             complement(4103..4558)
                     /locus_tag="VFU45_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359197.1"
                     /translation="MAEEPLHNMAGEPLPEGVDLFALGVRALGFASAAGATAIAIVLW
                     GVQSLVPTAPPGASPVAGPAFYLLVHGTPASMALAGATCWFLLRPVESYFRRGGLAVV
                     SAFLSFVISLITLPASALFGRPGLLAASLLFAVAMVLFGRSLRGWGRTA"
     gene            complement(4668..6065)
                     /gene="lpdA"
                     /locus_tag="VFU45_08765"
     CDS             complement(4668..6065)
                     /gene="lpdA"
                     /locus_tag="VFU45_08765"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU5359198.1"
                     /translation="MANQFDVIIVGGGPAGYVCAIRAAQLGLSTAVVEKDKLGGVCVN
                     IGCIPTKALLHSAMVANIVSHDARDLGVEVGAVKTDYGVAMKRSRKVAEQNSKGVEFL
                     MKKHKVTVIKGAGVLQAGRKVKVGGDVYEARKGVVLATGSRTKGIPQIGLELDRKLVI
                     SSDEALFLEQAPKSIAIVGAGAVGTEFADIFNAFGSKVWLVEALPRMLPVEDAESSDT
                     IAKLYRKRGIEVRAGATVKKATVGKDKVTLEVESAGQKETIEAEKVLMAAGRAVNTES
                     MGFKEAGVQLTDRGFVKVNLATLETTAPGVYAIGDVAGPPMLAHKGSREGVFVAELVA
                     GLKPHAIKYDNIPGVTYCQPEVASIGLTEEQCKAQKLDHQVGRFPFSANGRARASNET
                     DGFVKIIRGKKYGEILGAHIVGSHASEMIHELTVARENEFTVEEVDLAVHAHPTLSEA
                     IAEAALDSMGRVMHI"
     gene            complement(6181..>7213)
                     /locus_tag="VFU45_08770"
     CDS             complement(6181..>7213)
                     /locus_tag="VFU45_08770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU5359199.1"
                     /translation="TVPVANVVAWIGTPGESVGDGAPPAPARASMPASTPAAAPVAVG
                     TPAAPVARAPGAPAPAASGRVKASPLARKIAADRGLDLGAIAGSGPAGRVIARDLDGA
                     RPASTTRAAAPVAGGGYEDVPLSQIRKTIAKRLAQSIGPVPTFYLTTDVDMTSMWEAR
                     EALKGMGGDVKVSFNDIIIKATAAALLQHPACNAWWQDDHIRVWHEVHVGMAVAIPDG
                     LITPVIRHAEGKSLREIATEAKDLATRARERKLTPEEYTGSTFSISNLGMLEIEHFTA
                     IINPPEAGILAVGKIRDEAAVVEGQVRVRKAMRLTMSCDHRVIDGATGAQFLKTLKQM
                     LENPLALVW"
BASE COUNT         1015 a         2612 c         2520 g         1066 t
ORIGIN      
        1 tgaagcttcg ggcggccgtg gccgactcgg ggacggccgg gcccgccctc aggcgctgac
       61 cttccgggcc gccaggcggg aggacgggtg gcgcaccgcc tcctgggcgg ccgccaggtc
      121 cacgtgacac cgctcggtgc cctccagcac cccgacgacg tcggcgccgg acgtgccgat
      181 cgggcacgac agcgcaaaac tcatccccag cggcgactcg gccaggcgcg ccacggccat
      241 ctcccacttg agcccgccca tcacgggcga ccgcgtcacc acgaggggga tctcctcggc
      301 atggtcggtc accggcagca gcgccaggct cagcgcccgc gccagcgcct gccatccggg
      361 ccacccgtcg gtggcgtgga tcgcggtgaa gcgccatccc tcctcgcgcg ccaggtcagc
      421 gaaggcccgg agcaggtccg catccgccgg tgcgcagtcg cctccgggcg aggtgaggac
      481 cgcgccgccc gtctccgcga accatcggcg ggccgcggtc agggtggccg gcttgctccc
      541 ggggaacgcc gcgcaccgcc gcacgatcgc cacctggcgt tcggcctccc ggcgctgctc
      601 ggggaactcg gccgcctcga tggcgccggt gaacgccacc tccgcggcca ggtagcggcc
      661 cgcatcgcgg aggatcttgc cgcgcagcag cagcgtggca ccgtgggggt cgagcgcgaa
      721 ggcacgatcc accgccagca gcgcgcgcga agcctcgccc aggcgccagg cggcgtagcc
      781 ggtctcgtgc cacacgagcc cgaggtcggg gtcggcctcc gcggcccgct cgagggcggc
      841 aagcccgtcg cggtagcggc caagctccac ctgtgccgcg ccgacgagcg ccagggcgaa
      901 cgcctcgccg gggaagtggg tcgcggcgcg ctccgccagg aagagtgcgt cgtcgtggcg
      961 ccgttccgag cgcagccact cgaagtattc gccccaggct ctcctgccac cgggatcctc
     1021 ggccaggatg gcctgatagg cgtcctccgc ctccgcggat cgtccgtcgc gcgccagctg
     1081 ccgggcgcgc accagccgac gctcggcggc gctcgcgccg aacacgcgga actgggcggt
     1141 ggagtacatg gaggccaatg tacccgcggg accgacctcc gctaggagta tcgcgggccc
     1201 gcgccacttg cttccgaccc accgggctgt aggttcggtg ggttcgctcc aactttgatc
     1261 cgcccctcgc cggcctctcc ccatgcccaa tgatatcgcc acccgcgacc aggcccgcct
     1321 cctcggcgag ctgaaggaat tcctgcgcat cccgagcatc agcacccttc ccgaccacgc
     1381 tgcggactgc cggcgcgcgg cggactgggt catggaccac ctgcgccgcc tcggatgccc
     1441 ggtggtggaa ctgctggagg ggaaggggca cccgacgatc tgggccgagg ggccccaggt
     1501 gccgggcgcg ccgaccgtcc tgctgtacgg ccactacgac gtccagccgg ccgagccgct
     1561 ggaggagtgg gtgaccgggg ccttcgaccc gaccgtgcgg gacggcaacc tgtacgcccg
     1621 tggcgccgtc gacgacaagg ggcaggtgtt cacgctcctc aaggcgtacg aatcggtgct
     1681 gggtgccgac cgtcgccctc ccctcaacgt gcatttcatc atcgaggggg aggaggagtg
     1741 cggcggccgg gtcatcttcg atcgcctcaa ggcggagccg tggcgcacca aggccgacgc
     1801 ggtggtggtg gccgacatgg ggtactacgc gccgggggtc ccggcggtgt acaccgcgct
     1861 ccgggggctc gcctatgccg agatccaggt gcggacggcg gagagcgacc tgcactccgg
     1921 tgagtacggc ggcgtggcgc cgaacgccat cgagaccacc gtccggatcc tcgccgcgct
     1981 caaggacgcc agcggccgga tcaaggtgcc ggggatctac gaggcggtgc ggcgcccgag
     2041 ccggaccgag ctggcggggt ggaagaagct ccccttcgac cggcgcacgt acctcaagaa
     2101 gcaggtgacc gcgaaggcgc tcaccgggct ccagaagtac agcatccacg agcgcctctg
     2161 ggcgctgccc acgctcgaga tccacggcat ccggggcggc ttcgtgggtg aaggcgccaa
     2221 gaccgtgatc ccgtgtcagg cggtggccaa ggtgagcctc cggctggtgc ccggccacag
     2281 ctccgccagg gtctatcgcc agcttgcgct cgcggtcaag cgggtgacgc cgaagtgggc
     2341 cgacgtgaag gtcgtgccgc tccactccgg cgaaccggtg caggtcgagg tcgacgcgcc
     2401 ggccttcgcg gtgctcgacc gcgcgttcaa ggaggtggtt ggccggccca ccgtcgcggt
     2461 gcgcgcaggc gggtcgatcc cggtcgtctc cgccctggcg cagggcgggg cgccggtcct
     2521 cctcaccggt atcgggctgc cggacgaccg gcttcacgcc cccaacgaga aggtcacgct
     2581 ctcacaggtc tgggacggga tccggatgtt cgggaggttc ttcgagatga tggcggagaa
     2641 gaagtaggcg gacggacgca tggtcggacg accgcaaaac gatgaaaccc cgcttccaac
     2701 gagcggggtt tcgccatttc acgcgtatcc gaccatccga ccatgcgtcc acccgaacat
     2761 ccgcccgtcc gcttactcct ccgccacctg cttgccccgg ccgaactggg actcggtgcg
     2821 ggcgtgcagc tcgtcgacgc cgatcacggc gcggaccgac gccacgacgc ccgccacgag
     2881 gtcgtccgcc cagtagggat ccggctgctc cggcttggga agcaccttcc cccgctggtc
     2941 gaggaacccg atcgccgtgt tgatccactc cgtttgcgcg gtcgggacca gcgaccagga
     3001 accggtggcc cggagttcca tctcgctcag ttccttgagc tcctgctcga tcccggcaac
     3061 ggccgccttg gaggtcagcc gctcgacgag gagcttgagc atctgctccc gctcggactc
     3121 ctgcgcggtc gggagcggtg ccaggtagtg ccgctggtcc gccaccatct ccgccagccg
     3181 gatgcagacc gcccgcaccg ccggctggag cgccgtcggc acctcgcggg cccagctgcc
     3241 cgcggccttc tggatgtccg ccagcttctc gtcggccgcc tgcgccggag atcgcttcgt
     3301 cccgtctgcc atggctctaa ctaaagaccg ggctctgggg gaaatcaact ggtccggctc
     3361 ccggctgcta acgcggacgg gcccatccgt caatgtgccg cggacaccag ggccgggccg
     3421 agggtgcgct cgagggtgtc ggggaccggg gtccggtcga accgccgtcc gagcgcctcg
     3481 accaccgcgt gctgcatccg gtcccggagc gccgtcgggt cgccaccgat gcgcgccacc
     3541 tcacgggcca ctgatgtcat ctcgacgccg gggatgccgc acggcacgat ggcgtcgaac
     3601 ccgcgcaagt cgttggtcac gttgagggcg aagccgtgga acgtcaccca ctgcttcacg
     3661 tggatgccga tgcttgcgac cttacgcccc tccacccaca cgccggtgcg ccccggttcc
     3721 cgtccggcgg cgatgccgac ggtggccagc gcatcgatca gcacgccttc gagttcgcgg
     3781 aggtaccagt gcaggtcggg acggtgcccc tggaggtcga ggatggggta cccgaccagc
     3841 tgcccgggct cgtggacggt cacgtcgccg ccgcgctcca cctcgaacac ctcgacaccg
     3901 cgcagccgga gtgcgtctgg cggcacgggc aggctctcgg ggcgggtgcc gcgccccagg
     3961 gtgaccacgg ggtcgtgctc gacgaggagg aggaggtcgt cgtcgacggc gccggccacg
     4021 cgccggcggc agagctcccg ctgcagctcg agcgcgtcgc cgtacgggcg ccggccgagg
     4081 tcgatgaccc ggagcgcgcg ggtcacgccg tccgccccca gccgcggagg ctccggccga
     4141 agagcaccat cgccacggcg aagagcaggg aggcggccag gagcccgggg cgtccgaaca
     4201 gcgcgctggc cgggagggtg atgagcgaga tcacgaacga gaggaaggcg ctcaccaccg
     4261 ccaggccgcc gcggcggaag tacgattcca ccgggcggag gaggaaccag caggtggcgc
     4321 cggccagcgc catcgacgcc ggagtgccgt gcaccaggag gtagaacgcc ggccccgcca
     4381 cggggctcgc gccgggcggt gcggtcggca cgagtgattg caccccccac agcacgatgg
     4441 cgatggccgt ggcccccgcc gccgaggcga agccgagcgc ccgcacgccg agggcgaaga
     4501 ggtcgacccc ctcggggagg ggctcgccgg ccatgttgtg gaggggttcc tctgccatcg
     4561 tcactccggg gaggtgagcg gtgagccgtg agcggtaagc agtgagcggg ctgcagtccg
     4621 tggcctcgac gggcagcccg ctcaccgctc actgctcacc cctcacctca gatgtgcatc
     4681 acccgtccca tcgaatcgag cgccgcctcg gcgatcgcct ccgacagggt ggggtgggcg
     4741 tgcaccgcca ggtcgacctc ttcgacggtg aactcgttct cgcgcgccac ggtgagctcg
     4801 tggatcatct cagaggcgtg gctcccgacg atatgggcgc cgaggatctc gccgtacttc
     4861 ttgccgcgga tgatcttgac gaacccgtcg gtctcattgg aggcacgggc gcggccgttg
     4921 gcgctgaagg ggaagcggcc cacctggtgg tcgagcttct gcgccttgca ctgctcctcg
     4981 gtgaggccga tgctcgccac ttcaggctga caataggtga cgccggggat gttgtcgtac
     5041 ttgatggcgt gcggcttcag ccccgccacc agctcggcca cgaagacccc ttcgcggctc
     5101 cccttgtgcg ccagcatcgg cgggccggcc acgtcgccga tggcgtagac gccgggggcg
     5161 gtggtctcga gggtggcgag gttgaccttg acgaagccgc ggtcggtgag ctgcaccccg
     5221 gcctccttga agcccatgct ctcggtgttg accgcgcgac ccgcggccat cagcaccttc
     5281 tcggcctcga tggtctcctt ctgtcccgcc gactcgacct cgagcgtcac cttgtccttg
     5341 cccacggtgg ccttcttgac cgtggcgccg gcccgcacct cgatgccccg cttcctgtag
     5401 agcttggcga tcgtgtccga cgactcggcg tcctccacgg gcagcatccg cggcagcgct
     5461 tcgacgagcc acaccttgct gccgaaggcg ttgaagatgt cggcgaactc ggtgccgacc
     5521 gcccccgcgc cgacgatcgc gatcgacttc ggcgcctgtt ccaggaagag cgcctcgtcg
     5581 gagctgatga ccagtttccg gtcgagttcg agtccgatct gggggatccc cttggtgcgg
     5641 gagccggtgg cgaggacgac gcccttcctg gcctcgtaga cgtcgccgcc gaccttgacc
     5701 ttccggccgg cctggaggac gccggccccc ttgatcaccg tgaccttgtg cttcttcatc
     5761 aggaactcga cgcccttcga gttctgttcc gccaccttcc gtgagcgctt catggccacg
     5821 ccgtagtccg tcttgacggc gccgacctcg acgcccaggt ccctggcgtc gtgggagacg
     5881 atgttggcca ccatcgcgct gtggaggagg gccttggtcg ggatgcaccc gatgttgacg
     5941 cacacgccgc cgagcttgtc cttctcgacc acggcggtgc tgaggccgag ctgggcggcg
     6001 cggatggcgc acacgtatcc ggccggcccg ccgcccacga tgatgacgtc gaattggttc
     6061 gccacggggt ccctctgagg gatcaaggtt ggaagtcggt gagcggtgag ccgtaagaag
     6121 tgagcgggct gctgatgaag acgtctcctg agcacccgct cacctcttac ctctcacctc
     6181 tcaccatacc agggccagcg ggttctccag catctgcttg agcgtcttca ggaactgggc
     6241 gccggtggcg ccgtcgatca cgcggtggtc gcaggacatc gtcagccgca tcgcctttcg
     6301 cacccggacc tggccctcca ccaccgcggc ctcgtcgcgg atcttgccga ccgcgaggat
     6361 gccggcctcg ggcgggttga tgatggccgt gaagtgctcg atctcgagca tgccgaggtt
     6421 ggagatcgag aaggtcgacc cggtgtactc ctcgggggtg agcttccgct cccgtgcccg
     6481 cgtggcgagg tccttcgcct ccgtcgcgat ctcccggagc gacttcccct cggcgtggcg
     6541 gatgaccggg gtgatcagcc cgtcggggat cgcgacggcc atcccgacgt gcacctcgtg
     6601 ccacacgcgg atgtgatcgt cctgccacca ggcgttgcac gcgggatgct ggagcagcgc
     6661 cgcggcggtc gccttgatga tgatgtcgtt gaacgagacc ttgacgtcgc cgcccatccc
     6721 cttgagcgcc tcccgggcct cccacatcga cgtcatgtcc acgtcggtgg tgaggtagaa
     6781 cgtggggacc gggccgatcg actgcgccag gcgcttcgcg atggtcttcc ggatctggga
     6841 gagcggcacg tcctcgtagc cgcccccggc caccggtgcc gccgcccgcg tcgtcgatgc
     6901 cggccgggcg ccgtccaggt cccgggcgat gacacgaccg gccggccccg atcccgcgat
     6961 ggcgcccagg tcgagccccc ggtcggccgc gatcttccgt gcgagcgggg aggccttcac
     7021 ccggcccgac gccgccggcg ccggggcccc cggggcccgc gccaccggag cggcgggcgt
     7081 ccccacggcc accggcgccg ccgcgggtgt cgatgccggc atgctggccc gggccggcgc
     7141 cggcggcgca ccatcgccca ccgactcccc gggcgtcccg atccacgcca ccacgttcgc
     7201 caccggcacc gtc
//
LOCUS       DASUAP010000321         3460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1100992, whole genome shotgun sequence.
ACCESSION   DASUAP010000321 DASUAP010000000
VERSION     DASUAP010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100992"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFU45_08775"
     CDS             <1..526
                     /locus_tag="VFU45_08775"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEU5359200.1"
                     /translation="IVHGLQVTQLDAERLKERFGTAYEPLVPETDVIELPSTPGQGPR
                     SAARRTLSHIMHMRMQEVLEYAWDEITRAGYQQRLPAGIILTGGAAQAPGVIELAREV
                     CAVPVRVGVPGQGLSGLADSVESPRMAVPAGLVLYGARQMALGNVFGMGGHRGSASVE
                     KILAPVKKWLQDFF"
     gene            549..1739
                     /gene="ftsZ"
                     /locus_tag="VFU45_08780"
     CDS             549..1739
                     /gene="ftsZ"
                     /locus_tag="VFU45_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU5359201.1"
                     /translation="MKFELEEPLAQSANMKVLGVGGGGGNAVNRMIEEGLRGVQFVSV
                     NTDAQALAASRSDLKVQIGKKLTRGLGAGARPEIGRQAIEESRDEVLQMLQGADMVFV
                     TCGMGGGTGTGAAPIIAQLARDIGALTVGIVTKPFLFEGRKRIRQAEMGIAELRKNVD
                     TMIVVPNEKLLAVVGKQIPFQQALKKADEVLLHATQGISSLITSTGLINVDFADVRTV
                     MQNGGAALMGTGIGRGEHRAMEAAQQAIASPLLDDISIQGATGVLLNITGGGDLTLED
                     VNRIADIIHDAAGDDAEIIFGHTPDDAMQGEVRVTVIATGFDRQVTGEPGLRTPAGGA
                     GVLNFPAKRPSAAVPPPPPAVKPRPSQVPAAEPGAEAKRAEPRRPGAGDDMDIPTFIR
                     RQMD"
     gene            1739..2989
                     /locus_tag="VFU45_08785"
     CDS             1739..2989
                     /locus_tag="VFU45_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5359202.1"
                     /translation="MRRALRFVPAAVVVAALVAALAGRWPWPRLLSAAPIVVESPWVT
                     TTDTLHDGETLSALFARNGISGLSFERVSQELALDLRKLRPGTGFTFRRTPAESLPSR
                     VLFRTDPTRDLALEHSDSGWFGTAIPIDWRPETVRVDGSIENSLYLALDDHVPDSVLD
                     AGERIKLAWDLADIYQWQVDFTRDLRAGDHFTVALERLVAPDGEVRYGRVLAGDLVVD
                     GKHLTAYHFDNADGTVGFFDDQGRSLRRAFLIAPLQFRRISSSFSRSRFHPILGIWRH
                     HEGTDYAADYGTPVRAAGNGMVLRAGRYGGYGNLIELRHANGITTRYGHLSRFAGGIR
                     PGVRVTQGETIGFVGATGLATGPHLHYEFRVNGVARDPRRMDLGTGDPVPAKDRTVFQ
                     AERARLAALLQVSAPTGTLAAASR"
     gene            3006..>3460
                     /locus_tag="VFU45_08790"
     CDS             3006..>3460
                     /locus_tag="VFU45_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU5359203.1"
                     /translation="MELLPAIEVRSGRLIRSAGPGSPAVSAVGTDPVARAEELVWQGA
                     RWLHLVDLDRTFADGDNDATLARIVTRVGSYVRIQLAGGLRGRESLESVRHLEVARFV
                     VEAGALPERRVEEAIEILGVTRVAIGLDVRDGLVTQRDWKAIAGLSARDV"
BASE COUNT          489 a         1275 c         1231 g          465 t
ORIGIN      
        1 catcgtgcac gggctccagg tcacccagct cgatgcggag cgcctgaagg agcggttcgg
       61 caccgcatac gagccgctgg tgccggagac cgacgtgatc gagctgccca gcacgccggg
      121 gcaggggccc cggagcgcgg cccggcggac gctgtcgcac atcatgcaca tgcggatgca
      181 ggaggtgctg gagtacgcct gggacgagat cacccgggcg ggctaccagc agcggctccc
      241 ggcgggcatc attctgaccg gcggcgcggc ccaggccccc ggcgtcatcg agctggcgcg
      301 cgaggtgtgc gcggtgccgg tgcgggtcgg ggtgccgggg caggggttgt cggggctcgc
      361 cgacagcgtg gaatcgcccc ggatggcggt gccggcgggg ctcgtgctct acggcgcgcg
      421 gcagatggcg ctggggaacg tgttcgggat gggcgggcac cgtgggagtg cgtcggtgga
      481 aaagatcctt gcccccgtca agaaatggct gcaggacttc ttctgagatc acccggaggg
      541 gagcgcccat gaaattcgag ctcgaggagc cgctcgcgca gagcgcgaac atgaaggtcc
      601 tcggcgtcgg cggcggcggc ggcaacgccg tcaaccgcat gatcgaagag ggcctgcgcg
      661 gcgtgcagtt cgtgtcggtc aacaccgacg cccaggcgct ggcggcgtcc cggtcggacc
      721 tcaaggtcca gatcggaaag aagctcaccc gcgggctcgg cgcgggggcg cgtcccgaga
      781 tcggccgtca ggccatcgag gagagccgcg acgaggtcct gcagatgttg cagggcgcgg
      841 acatggtgtt cgtcacctgc gggatggggg gcggcaccgg gaccggggcg gcgcccatca
      901 tcgcgcagct ggcgcgggac atcggcgccc tcacggtggg gatcgtcacc aagccgttcc
      961 tcttcgaggg gcgcaagcgg atccggcagg ccgagatggg catcgccgag ctgcggaaga
     1021 acgtcgacac catgatcgtc gtgcccaacg agaagctgct ggccgtggtc gggaagcaga
     1081 tcccgttcca gcaggcgctc aagaaggccg acgaggtgct gctccatgcc acgcagggca
     1141 tctcctcgct catcaccagc accgggctca tcaacgtcga cttcgcggac gtccggacgg
     1201 tgatgcagaa cggcggcgcc gccctcatgg gcaccggcat cggccgcggc gagcaccgcg
     1261 cgatggaggc ggcccagcag gccatcgcga gcccgctgct tgacgacatc tcgatccagg
     1321 gcgccaccgg cgtgctgctc aacatcaccg gcggcggcga cctcaccctc gaggacgtca
     1381 accgcatcgc cgacatcatc cacgacgccg ccggcgacga cgccgagatc atcttcgggc
     1441 atacgccgga tgacgcgatg cagggcgagg tgcgggtgac cgtgatcgcc accggcttcg
     1501 accgccaggt caccggcgag ccgggcctcc gcacgccggc cggcggcgcg ggcgtcctca
     1561 acttccccgc caagcgcccg tcggcggcgg tccccccgcc gcccccggcg gtcaagccgc
     1621 ggccgtccca ggtcccggcc gccgagccgg gcgcggaggc caagcgcgcc gagccccggc
     1681 gccccggcgc cggggacgac atggacatcc cgaccttcat ccggcgacag atggattgat
     1741 gcgccgggcc ctgcggttcg tgccggcggc ggtggtcgtg gccgcgctcg tggcggcgct
     1801 cgccggtcgc tggccctggc cgcggctcct gagcgcggcg ccgatcgtcg tggagtcccc
     1861 ctgggtcacg accaccgaca ccctgcacga cggtgagacc ctctcggccc tcttcgcgcg
     1921 caatggcatt tccggcctgt cgttcgagcg ggtgagccag gagctcgcgc tcgacctccg
     1981 gaagctccgg cccgggaccg ggttcacctt ccgccgtacc cccgccgagt ccctcccgtc
     2041 ccgcgtgctc ttccgcaccg accccacccg cgacctcgcg ctcgagcaca gcgacagcgg
     2101 atggttcggc acggcgatcc cgatcgactg gcgcccggag acggtgcggg tggacgggag
     2161 catcgagaac tcgctctacc tggcgctcga cgaccacgtt cccgactcgg tgctcgacgc
     2221 cggcgagcgg atcaagctcg catgggacct ggccgacatc taccagtggc aggtggactt
     2281 cacccgcgac ctccgggcgg gcgaccactt caccgtggcc ctcgagcggc tcgtggcccc
     2341 cgacggcgaa gtgcgctacg gccgcgtgct ggccggcgac ctcgtggtcg acgggaagca
     2401 cctcactgcg taccatttcg acaacgcgga cggcacggtg ggcttcttcg acgaccaggg
     2461 ccggtcgctc cgccgcgcgt tcctgatcgc cccgctccag ttccgccgga tctcctccag
     2521 cttcagccgc tcccgcttcc acccgatcct gggcatctgg cgccaccacg aaggcaccga
     2581 ctacgccgct gactacggga cgccggtccg cgccgccggc aacggcatgg tgctccgggc
     2641 cggccgctac ggcggctacg gcaacctgat cgagctgcgg cacgccaacg ggatcaccac
     2701 ccgttacggg cacctgtcac gcttcgcggg cggcatccgc cccggcgtgc gggtcacgca
     2761 gggcgagacg atcggtttcg tcggggccac cggcctcgcc accggccccc atctgcacta
     2821 cgagttccgg gtcaacggcg tcgcccgcga cccgcgccgg atggatctcg gcaccggcga
     2881 ccccgtcccc gccaaggacc gcacggtgtt ccaggccgaa cgcgcccggc tcgcggcgct
     2941 cctgcaggtg agcgctccca ccggcaccct cgccgccgcc tcgcgctgac gacggcgccc
     3001 ccgtcgtgga actcctcccc gccatcgaag tccgcagcgg ccggctgatc cggtcggcag
     3061 gccccggttc gcctgcagtg tcggccgtgg gcaccgaccc cgtcgcgcgg gcggaggaac
     3121 tggtgtggca gggtgcccgc tggctccacc tcgtcgacct cgaccggacc ttcgccgacg
     3181 gcgacaacga cgccacgctc gcccggatcg tgacccgcgt cggctcgtac gtccgcatcc
     3241 agctggccgg cgggctccgc ggccgcgaga gcctggagtc ggtgcgccac ctcgaggtcg
     3301 cgcgtttcgt ggtcgaggcc ggcgccctgc ccgagcggcg ggtcgaggag gcgatcgaga
     3361 tcctcggcgt cacccgcgtg gcgatcggcc tcgacgtgcg tgacggcctg gtgactcagc
     3421 gcgactggaa ggcgatcgcc ggcctctccg cccgggacgt
//
LOCUS       DASUAP010000322         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_583395, whole genome shotgun sequence.
ACCESSION   DASUAP010000322 DASUAP010000000
VERSION     DASUAP010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583395"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VFU45_08795"
     CDS             complement(<1..1059)
                     /locus_tag="VFU45_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359204.1"
                     /translation="MRRVAFVGPGEEGDAAWRWLAGLREVASHCYPGIAAIPLVNADV
                     VWLHGEVAPDAVLVPWLERGGRLLCTGPAALIPSALGLESVAPDERRVSTWHHAEDEF
                     WFEEFRSFVAFPHVRGLAGFGPHPLFEGLHQGTYTWAPAEGEPFDWVTYRQARPAEGR
                     VVAVERSFIHLNAGRVIAWEQGVGAGGILAIGAYTHFNAADPLLAPQLRALVANAVVG
                     EAIPGPRRTAMSSTWPAPGGRARIDDLLPPVELPDLTGAWRESRSPLAIVSDASADDP
                     WTLAGRRMLLVGSEQEGLREVWAHPHRVVRDVAVSVGGRPARGVSARIAPDQVGRVLA
                     DGGVERWTAALEHPAMLWD"
     gene            complement(1056..3251)
                     /locus_tag="VFU45_08800"
     CDS             complement(1056..3251)
                     /locus_tag="VFU45_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359205.1"
                     /translation="MRPSIPAPSRPDKISLGSGDGLIWAPRFPAWHDTPGFWDEAHFL
                     QYPLQPLFALSFVGPDGNADLLRARRLRWTPTALQLDYAWPGVAAVETRSAPGDRRLV
                     SEWSVTNRTRRAIELDVVLWAVVPGESVGPRGATATDRGLRLVREVTDWAEESREFAL
                     DYQLGGATSREAVRSELSGASPRFDLTPFYDTWPGRRRLSGSLRLEGIDRTGSVYIAL
                     HRRLRVAARGAASFSASVRVTPLGVRVPRLPATRRIAARSAGEGWQRWLAGGPSFRCG
                     DRHLSAFWDHRWWGLRLHTVPAGTGQFRASAVCEGPGFFHQPISYSAMCHARELRWRH
                     AATECQGVIDAFFDHQAPDGKLPGRVYLDHLSRTDFYHADWGGAIEDVLDTHPDPRWT
                     RALYPKLARYATWLTDTRDAEGSGMIDVVDQYESGQEYMSRYQAVDPGADRYGWEERI
                     RLKGIDVTVYAYRLYVLLARLAPEARMAGVWRALADRTRAAVRGTMWDPARQMFSDVD
                     PRTGRRTGVKAAVCFYPYLTDLVDGSHIEGMGRHLFDRREFWTPWPVPSSSVDDPLFD
                     PDARWKGKRHVCPWNGRVWPMTNSHVADALARVVRLHRPAWAPKLVELLGKFVRMMSF
                     DQEPLRPNSFEHYHPFNGRGSVYRGVDDYQHSWVNDLIVRHVAGVLPQGEEGVVLDPM
                     PFGAPVRLTGLRVAGHRIDVEVAAGRYRARVDGRPAAAGRIGAPSEVRW"
     gene            complement(3375..>3746)
                     /locus_tag="VFU45_08805"
     CDS             complement(3375..>3746)
                     /locus_tag="VFU45_08805"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5359206.1"
                     /translation="RGRNVLLAFFPLAFTSTCTAELCEFSTDYSQFQANDTVVLPISV
                     DSVPTLKAFKAQERMTVELLSDFKRDVSRRYGVLLEDRFFASRAYILIDRQGVVRWTH
                     NERELGHKRSTAELMTELGKL"
BASE COUNT          572 a         1380 c         1310 g          484 t
ORIGIN      
        1 gtcccagagc atcgccgggt gctcgagggc ggcggtccag cgctccacgc cgccatccgc
       61 caggacgcgc cccacctggt cgggcgcgat gcgcgccgag accccgcgag ccggccgacc
      121 gcccaccgag accgcaacgt cgcggacgac gcggtgcggg tgcgcccaga cctcgcggag
      181 cccctcctgc tcgctgccca cgaggagcat ccgccggccc gccaacgtcc acggatcgtc
      241 cgcgctcgcg tcgctcacga tcgcgagcgg cgagcgcgac tcccgccacg cgccggtcag
      301 gtcgggtaac tcgaccggtg gcaggaggtc gtcgatgcgc gcccggcccc ccggtgcggg
      361 ccaggtgctc gacatcgcgg tccggcgcgg gccggggatc gcctcgccga ccacggcgtt
      421 cgcgacgagc gcccgcagct gcggcgccag gagcggatcc gccgcgttga aatgggtgta
      481 ggcgccgatc gcaaggatgc cccccgcgcc gacgccctgc tcccacgcga tcacccgccc
      541 ggcgttcaga tggatgaagg accgctcgac cgcgaccacg cgtccttccg ccggccgcgc
      601 ctgccggtac gtcacccagt cgaagggctc gccctcggcc ggcgcccagg tgtaggtgcc
      661 ctggtgcagg ccctcgaaga gcggatgcgg gccgaacccc gccagcccgc gcacgtgggg
      721 gaacgcgacg aacgaccgga actcctcgaa ccagaactcg tcctcggcat gatgccaggt
      781 cgacacgcgt cgctcatccg gcgccaccga ttcgaggccg agggccgagg ggatgagtgc
      841 cgcagggccg gtgcagagga gccggccgcc gcgttccagc cacggcacca gcaccgcgtc
      901 gggcgcgacc tccccgtgca gccagacgac gtccgcgttc accagcggga tggcggcgat
      961 accagggtag caatgggacg cgacctcccg gagcccggcg agccagcgcc aagcggcatc
     1021 gccctcctcg ccggggccga cgaacgccac ccgcctcacc agcgcacctc cgacggcgct
     1081 ccgatgcgcc ccgcagcggc cggcctgcca tcaacgcgcg cccggtaccg acccgcggcc
     1141 acttcgacgt cgatccggtg tccggccacg cggagcccgg tgagccgcac cggcgctccg
     1201 aacggcatcg gatcgaggac cacgccctct tccccctgcg gaagcacgcc ggcgacgtgg
     1261 cgcacgatga ggtcgttgac ccacgagtgc tggtagtcgt ccaccccgcg gtacacgctg
     1321 ccgcggccgt tgaatgggtg gtagtgctcg aagctgttgg ggcggagcgg ttcctggtcg
     1381 aagctcatca tccggacgaa cttgcccagc agttcgacca gcttcggcgc ccacgccggg
     1441 cggtggaggc ggaccacccg ggcgagcgca tcggcgacgt ggctgttggt catcggccac
     1501 acccggccgt tccacgggca tacgtggcgc ttccccttcc agcgcgcgtc cgggtcgaag
     1561 agcgggtcgt ccacactcga actcggcacc ggccacggcg tccagaactc ccggcggtcg
     1621 aagaggtgcc gccccatgcc ctcgatgtgt gagccatcga cgaggtcggt gaggtacgga
     1681 tagaagcaca ccgccgcctt gacgccggtc cgacgtccgg tgcgcgggtc gacgtcgctg
     1741 aacatctgcc gggcgggatc ccacatcgtt ccccggaccg cggcgcgggt ccggtccgcg
     1801 agcgcccgcc agacgccggc catgcgcgcc tcgggcgcca gacgtgcgag caggacgtac
     1861 agccggtagg cgtacacggt gacgtcgatg cccttcaacc ggatgcgctc ttcccaacca
     1921 tatcggtcgg cgccgggatc caccgcctgg taccgcgaca tgtactcctg cccggactcg
     1981 tactggtcca ccacgtcgat catccccgac ccttcggcgt cgcgggtgtc ggtgagccag
     2041 gtcgcgtaac gcgcgagctt cgggtagagg gcccgcgtcc agcgcggatc gggatgggtg
     2101 tcgaggacgt cctcgatggc cccgccccag tcggcgtggt agaagtcggt gcgtgacagg
     2161 tggtcgaggt acacccggcc cggaagcttc ccgtccggcg cctgatggtc gaagaacgcg
     2221 tcgatgacgc cctggcactc cgtcgccgcg tgccgccagc gcagctcgcg cgcgtggcac
     2281 atcgcagagt agctgatcgg ctggtggaag aagcccggcc cctcgcagac cgccgacgcc
     2341 cggaactgcc ccgtcccggc cggcaccgtg tgcagccgga gcccccacca ccggtgatcc
     2401 cagaatgcgc tgaggtgccg gtcaccgcac cggaaggacg gaccgcccgc gagccagcgt
     2461 tgccacccct cccccgccga ccgggcggcg attcgccgcg tcgccgggag gcggggcaca
     2521 cgcacgccca gcggagtgac ccggaccgag gccgagaatg acgccgcccc gcgggccgcg
     2581 acccgcagtc gacggtgcag ggcgatgtac actgagcccg tccggtcgat cccctcgagg
     2641 cgaagggatc ccgacagtcg ccgccggccc ggccaggtgt catagaatgg cgtcaggtcg
     2701 aatcgaggcg aagcgccgct cagctcactc cgcaccgcct cgcggctggt ggcgccgccg
     2761 agctggtagt cgagcgcgaa ctcccgcgac tcttcggccc agtcggtcac ttctcgcacg
     2821 agacgcaatc cgcgatccgt ggcggtcgcc ccgcgggggc ctacggactc tcccgggacc
     2881 accgcccaga ggaccacgtc aagttcgatg gcccggcggg tgcggttcgt gaccgaccat
     2941 tccgacacca ggcgccggtc gcccggggcg gatcgggtct cgaccgccgc cacccccggc
     3001 cacgcgtagt caagctggag cgccgtgggc gtccagcgaa ggcgccgcgc gcggaggaga
     3061 tcggcgttgc catcggggcc cacgaagctc agggcaaaga ggggctgcag ggggtactgg
     3121 aggaagtggg cttcatccca gaagccgggc gtgtcgtgcc aggccgggaa gcggggcgcc
     3181 cagatcaggc catccccgga gccgagggag atcttgtccg ggcgggaggg cgctgggatg
     3241 gacggacgca tggtcggacg ggcggcggga cggatgggcg cgggcacgcc agtaacctaa
     3301 ccggcgcagt gcggccgacc accaccgttc gggtccgacc atccgcacga ccacgtgccc
     3361 atccgactgt gcgcctacag tttccccagc tccgtcatca gctcggcggt gctgcgcttg
     3421 tggccgagct cgcgctcgtt gtgggtccag cggacgacgc cctgcctgtc gatcaggata
     3481 taggcccggc tggcaaagaa ccggtcctcg aggaggacgc cgtaccgccg gctcacgtcg
     3541 cgcttgaagt cgctcagcaa ctcgaccgtc atccgctcct gggccttgaa cgccttcagg
     3601 gtcgggaccg agtcgacgct gatcgggagc acgactgtgt cgttcgcctg gaattgcgag
     3661 tagtcggtgc tgaactcgca gagctccgcc gtgcaggtgc tggtgaacgc cagcgggaag
     3721 aaggccagca gcacgttccg cccgcg
//
LOCUS       DASUAP010000323         4499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1183519, whole genome shotgun sequence.
ACCESSION   DASUAP010000323 DASUAP010000000
VERSION     DASUAP010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183519"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFU45_08810"
     CDS             <1..191
                     /locus_tag="VFU45_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359207.1"
                     /translation="TELKAKGGMLAGPELVQVRQRVADLEGENQALRTRLDAATERVR
                     GLIQRLAFLERDLEDGAA"
     gene            188..526
                     /locus_tag="VFU45_08815"
     CDS             188..526
                     /locus_tag="VFU45_08815"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU5359208.1"
                     /translation="MTAPKQAVKVWIGGDEYTVRSELPAEYTKEVAAYLDAALKRVRE
                     MLPTLDAQKAAVLAALAITDELFQARRGDREVAARLTALADDVARILPPAKRGERTSA
                     AGALTLNDRS"
     gene            572..2152
                     /gene="rny"
                     /locus_tag="VFU45_08820"
     CDS             572..2152
                     /gene="rny"
                     /locus_tag="VFU45_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU5359209.1"
                     /translation="MSNDLLFSMLGGLVAGLLVSLLLTFLYIRREKRSARGIEASARA
                     EAEKLVAEAARTATAAREELMLEGRSALLKLREELEQEVQRRREEQGRLERRVEERDR
                     ELEQRGRQVQQQERVFLDRETALKRREQELEQRAQELDQRRGEEQARLEKLAGLTAEE
                     AKRELVHRVEDEARSEAAALARDIKEQAKRGAERDARRIIAMSIQRVAAEATAESTVA
                     AVTLPSDEMKGRIIGREGRNIRAFETATGVDVIIDDTPDTVVISCFEPVRREVARRAL
                     EALIADGRIHPGRIEEVVAKAQKELEAQLVELGEQAAYETGVHGLHPELIKYVGRMRY
                     RTSYGQNILEHSKEVAFLAGMMAAELKLDIALAKRGALLHDVGKVLTHEHEGTHVELG
                     VELARKFGETAAVVGIIHAHHDDVPHESAESVLVQAADAISGSRPGARREAFETYVKR
                     LTKLEEIANGYAGVEKTNVIQAGREVRVIVTPDRVDDAQAAQLCESIARRIEGEVQYP
                     GQIKVVVVRESRAVDFAR"
     gene            2149..3030
                     /gene="folD"
                     /locus_tag="VFU45_08825"
     CDS             2149..3030
                     /gene="folD"
                     /locus_tag="VFU45_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEU5359210.1"
                     /translation="MTARIIDGTALARTLRAEMADDVRRLTALGRRPGLAAVLVGDDP
                     ASQSYVRSKGKACEEAGMLSRTVLLPATTPEAELLALIDRLNVDPEIHGILVQLPLPK
                     QIDTERVLLHVSPAKDVDGFHPINVGRLVLGDPKAFRPATPWGIIQMLLREKIETKGA
                     HAVVLGRSTIVGRPMANLLLQDAPGGNATVTVCHSKTRDLPSVTRTADILIAAIGRAE
                     FVTREMVKPGAVVIDVGINRVEDASKPKGYRLTGDVAFGPVSEVASAITPVPGGVGPM
                     TIAMVLKNTIQAAGSGA"
     gene            3047..4201
                     /gene="xseA"
                     /locus_tag="VFU45_08830"
     CDS             3047..4201
                     /gene="xseA"
                     /locus_tag="VFU45_08830"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU5359211.1"
                     /translation="MTDLFSRSDQPAEGALTVAELDRRCRRLLEGEIGPTWVKGEVSG
                     LKAYQSGHWYFTLKDQDAQIRCVMWRANAVKAGPPPADGAQVFLFGTPTVWEERGEFR
                     LTVTRLLALEGVGAQQLAFERTKKALEKDGLFDPARKRALPAFARTVAVVTSLDGAAL
                     HDIITVARKRWPAVRLIAIGTKVQGGEAEAEVVAALKRVDRLDGVDVCIVGRGGGARE
                     DLEVFNREAVCRALAAVRVPTVSAVGHETDISLTDLVADVRAATPSAAAELVVPDRAI
                     WWHRAQDLATRLAGALTQRGRLARERLARSADRLEAALDDLLLDRRRHADRLAAQLDA
                     LSPLKVLGRGYAVPTGAEGRVLKTVADFGPGAPFTLRVSDGSVPARVEPT"
     gene            4213..4458
                     /gene="xseB"
                     /locus_tag="VFU45_08835"
     CDS             4213..4458
                     /gene="xseB"
                     /locus_tag="VFU45_08835"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU5359212.1"
                     /translation="MPRKAADPAAETPSLQDELRRLEEIVRELEGQELDLDRAIALFE
                     EGVGRLRVAREQLAAVETRVQKVLEDATGELRLVDLD"
BASE COUNT          686 a         1600 c         1663 g          550 t
ORIGIN      
        1 tcaccgagct caaggccaag ggggggatgc tcgccggacc cgagctggtc caggtgcgcc
       61 agcgcgtggc cgatctcgaa ggcgagaacc aggcgctccg cacccggctc gacgccgcga
      121 ccgaacgggt gcgcgggctg atccagcgcc tcgcattcct ggagcgcgac ctcgaggacg
      181 gtgccgcgtg acggccccca agcaggcggt caaggtctgg atcggcggcg acgagtacac
      241 cgtccggtcc gagctcccgg ccgagtacac caaggaggtc gcggcgtacc tcgacgcggc
      301 cctgaagcgg gtgcgggaga tgctcccgac ccttgatgcc cagaaggcgg cggtgctggc
      361 ggcgctggcc atcaccgacg agctgttcca ggcccggcgc ggcgaccgcg aggtggcggc
      421 ccgcctcacg gcgctggcgg acgacgtggc gcggatcctc ccgcccgcca agcgcgggga
      481 gcggacctcc gccgcgggcg cattgaccct gaacgatcga tcctgaccgg ttgaccatcc
      541 cgcggccccc tgccgcgtgg gagatagatc gatgtccaac gacctgctgt tctcgatgct
      601 gggcggcctc gtcgcaggcc tgctcgtctc cctgctgctc actttcctct atatccggcg
      661 cgagaagcgg agcgcgcgcg ggatcgaggc gtccgcccgc gcggaggccg agaagctggt
      721 ggccgaggcg gcccggacgg ccaccgccgc tcgcgaggag ctgatgctcg aggggcggag
      781 cgcgctcctc aagctccggg aggagctgga gcaggaggtc cagcgccggc gggaggagca
      841 ggggcggctg gagcggcggg tggaggagcg cgaccgggag ctcgagcagc gcgggcgcca
      901 ggtgcagcag caggagcgcg tcttcctgga ccgggagacg gcgctcaagc ggcgggagca
      961 ggagctggag cagcgcgccc aggagctcga ccagcgccga ggcgaagagc aggcccggct
     1021 ggagaagctg gccggcctca ccgccgagga ggccaagcgg gagctggtac accgggtgga
     1081 ggacgaggcc cggagcgagg cggcggcgct cgcgcgcgac atcaaggagc aggccaagcg
     1141 gggtgcggag cgcgatgccc gccgcatcat cgcgatgagc atccagcggg tggccgccga
     1201 ggccacggcc gagagcaccg tggccgccgt gaccctcccg agcgacgaga tgaagggccg
     1261 gatcatcggg cgcgagggcc ggaacatccg cgccttcgag accgccaccg gcgtcgacgt
     1321 catcatcgac gacacgccgg acacggtggt catctcctgc ttcgaaccgg tgcggcgtga
     1381 agtggcgcgc cgggcgctcg aggcgctcat cgccgatggc cgcatccacc cgggccggat
     1441 cgaggaggtc gtcgccaagg cccagaagga gctcgaggcc cagctggtgg aactgggcga
     1501 gcaggccgcc tacgagaccg gcgtccacgg gctccatccg gagctcatca agtacgtggg
     1561 ccggatgcgc taccgcacct cctacggcca gaacatcctc gagcactcga aggaagtggc
     1621 gttcctcgcc gggatgatgg cggcggagct caagctcgac atcgccctcg ccaagcgtgg
     1681 cgcgctcctc cacgacgtgg gcaaggtgct gacccacgag cacgagggca cccacgtgga
     1741 gctcggcgtg gagctggcgc ggaagttcgg cgagacggcg gcggtggtcg gcatcatcca
     1801 cgcccaccac gacgatgtcc ctcatgagag cgccgagtcg gtgctggtcc aggccgcgga
     1861 cgccatcagc ggctcccgcc ccggcgcccg gcgcgaggcg ttcgagacct acgtcaagcg
     1921 gcttaccaag ctcgaggaga tcgccaacgg gtacgccggc gtcgagaaga ccaacgtcat
     1981 ccaggcgggg cgcgaggtgc gggtcatcgt gacgccggac cgggtggacg acgcgcaggc
     2041 ggcccagctc tgcgagtcga tcgcccggcg catcgaggga gaggtccagt acccgggcca
     2101 gatcaaggtc gtggtcgtcc gcgagagccg ggccgtggac tttgcccgat gaccgcacgg
     2161 atcatcgacg ggacggcgct cgccaggacc ctccgcgccg agatggccga cgacgtccgg
     2221 cggctcaccg ccctcggccg ccgacccggt ctcgccgccg tcctcgtggg cgacgatccc
     2281 gccagccagt cgtacgtccg cagcaagggg aaggcgtgcg aggaggcggg gatgctctcc
     2341 cgcacggtgc tgctcccggc caccacgccg gaggcggagc tcctggcgct catcgaccgg
     2401 ctcaacgtcg accccgagat ccacgggatc ctggtccagc tcccgctccc gaagcagatc
     2461 gacaccgaac gcgtgctgct gcacgtttcg ccggccaagg acgtggacgg cttccacccg
     2521 atcaacgtcg gacggctcgt cctcggcgac ccgaaggcgt tccgccccgc cactccgtgg
     2581 ggcatcatcc agatgctgct ccgcgagaag atcgagacca agggggccca tgcggtcgtc
     2641 ctcggccgct cgacgatcgt cggccgcccg atggccaacc tcctcctgca ggacgcgccg
     2701 ggggggaacg ccaccgtcac cgtctgccac tccaagaccc gcgacctgcc ctccgtcacc
     2761 cgtaccgccg acatcctcat cgccgccatc ggtcgggcgg agttcgtcac ccgcgagatg
     2821 gtcaagcccg gcgccgtcgt gatcgacgtc ggcatcaacc gggtggagga cgcgtcgaag
     2881 cccaaggggt atcgcctgac cggggacgtc gccttcggcc cggtgtcgga ggtggcctcc
     2941 gccatcacgc cggtccccgg cggcgtcggg ccgatgacga tcgcgatggt gctcaagaac
     3001 accatccagg ccgcggggtc gggcgcgtag gcacgcccga cgcgccatga ccgacctctt
     3061 ttcccgctcc gaccagcccg ccgagggcgc cctcaccgtc gccgagctcg accgccgctg
     3121 ccggcggctc ctcgaagggg agatcggccc gacctgggtc aagggcgagg tctccggcct
     3181 caaggcctac cagagcggcc actggtactt caccctcaag gaccaggacg cgcagatccg
     3241 gtgcgtgatg tggcgggcca acgcggtcaa ggcgggcccg ccgcccgccg acggcgcgca
     3301 ggtcttcctc ttcggcacgc ccacggtatg ggaggagcgc ggcgagttcc ggctcaccgt
     3361 cacccggctg ctcgcgctcg agggcgtcgg cgcccagcag ctcgccttcg agcgcaccaa
     3421 gaaggcgctc gagaaggacg ggctcttcga tcccgcgcgg aagcgggcgc tccccgcctt
     3481 cgcgcggacg gtggcggtgg tcacgagcct cgacggcgcc gcgctgcacg acatcatcac
     3541 cgtggcgcgc aaacgctggc cggcggtgcg cctgatcgcg atcggcacca aggtgcaggg
     3601 gggcgaagcg gaggccgagg tggtcgcggc gctcaagcgg gtcgaccggc tggacggcgt
     3661 cgatgtgtgc atcgtggggc gtggcggcgg ggcgcgggag gacctcgagg tcttcaatcg
     3721 cgaggcggtc tgccgcgcgc tcgcggcggt ccgggtgccg acggtctcgg cggtgggaca
     3781 cgagaccgac atctcgctca ccgacctcgt ggccgacgtg cgcgccgcca cgccgtccgc
     3841 cgccgcagag ctggtggtgc ccgatcgcgc catctggtgg caccgggcgc aggacctggc
     3901 cacccggctc gccggggcgc tcacccagcg cgggcgcctc gcccgggagc ggctggcgcg
     3961 gagtgcggac cggctcgagg cggccctcga cgacctgctc ctcgaccggc gccgccacgc
     4021 cgatcgcctc gccgcccagc tcgacgccct gagtccgctc aaggtcctgg gccgtggcta
     4081 cgcggtgccc accggcgccg agggcagggt gctcaagacc gtcgcggact tcgggcccgg
     4141 tgcgccgttc accctccggg tgagcgacgg gtcggtgccg gcccgcgtgg aaccgacata
     4201 aggagggccg cgatgccaag gaaagccgcc gatcccgccg ccgagacgcc gagcctgcag
     4261 gatgagctcc ggcgcctgga ggagatcgtc cgcgagctcg aggggcagga actcgacctc
     4321 gaccgcgcga tcgcgctctt cgaggagggg gtgggccggc tccgcgtggc gcgcgaacag
     4381 ctcgccgcgg tcgagacccg ggtgcagaag gtcctcgagg acgccaccgg ggagctccga
     4441 ctcgttgacc tcgactgagc cgcccgtggg cgcggaagcg ctcttggccg aggcgcgca
//
LOCUS       DASUAP010000324         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_446381, whole genome shotgun sequence.
ACCESSION   DASUAP010000324 DASUAP010000000
VERSION     DASUAP010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446381"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..961
                     /locus_tag="VFU45_08840"
     CDS             218..961
                     /locus_tag="VFU45_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359213.1"
                     /translation="MDRSTRLGRTIFAVGAAALGLQHLITGNFGPGLEAVPAWVVGRT
                     ELAYLTGLALCLGALGVLAAVRRRQGAVLVAIAFSALFVFLQWPLLIYHVTDPNVWTT
                     VFETLALSGAAWTLVTSTAAPGEKRVPPALGRLAYGLALPAFGILHFKYAHFVGTLIP
                     AWIPLRTAWAVFIGICFVAAGLAIVTRIRARLAATLLGAMFGSWVVLLHIPLVITSHG
                     AGGKWTSLFVATMMCGGAWMVGGQDAFGA"
     gene            complement(1158..>2631)
                     /locus_tag="VFU45_08845"
     CDS             complement(1158..>2631)
                     /locus_tag="VFU45_08845"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359214.1"
                     /translation="FQIESRAQIQVIRRTRPRNIADLAVEVAIVRPGPVVGGAVNPYV
                     KRREAQRVAEAAGRPYQPPVEHPLLRDALDETLGVILYQDQVLEVCQAMAGFTAGQAE
                     ALRRAMSRRRSKEMMEQFRVEFLSGARAKGVSDEVAEQVFGHVTAFSEFGFPKAHAAA
                     FALLAYQSAWLRHYHPVEYYTALFNNQPMGFYSLDALTRDARRGGVEILLPDLERSDV
                     WCVREGGSVRVGLGFVRGWNEETATAVVLERERNGPYRSVGDFVRRAPAELKRAAIEH
                     LVWVGGCDHFGLTRRDLIWQVGLWLSPTEPGTDGPTRRRQMELALDHPHAHIRFGELD
                     AGERLVAEYATLGFAPTTHPFALFRGALPPYVVRSDRLKELRHGSKVEVAGMVVARQR
                     PGTAKGFVFVLLEDEAGMMNAILTPDVYERDRTTVRGEPFLWIAGKLSREGPGVNILA
                     EGVRPLALKGRGPEDTPMKRAPTELLQALRQGGADSHDWG"
BASE COUNT          356 a         1021 c          843 g          411 t
ORIGIN      
        1 actaacgcgg tgaacagtga acgggggctc cggtcgggat cggggccccg tttcgttcgg
       61 gcgcgtggac gcacagtcgc atggacggat ggtgccggtc gcgccttcat ccgaccatcc
      121 ggctatgcgc ctatccgtcc ccgcccctgt gcacccccca cccccgtcca cgtccttccc
      181 cggaccaccg accacgaccg acctccgggg acccgccatg gaccgttcga ctcgtctggg
      241 acgcaccatc tttgccgtcg gcgccgcagc actcggcctc cagcacctca tcaccggcaa
      301 cttcggcccc gggctcgaag ccgtaccggc gtgggtcgtg ggacgcaccg aactggcgta
      361 tctcacgggc ctggccctct gcctcggcgc gctgggtgtg ctcgctgccg tgcgccggcg
      421 ccagggcgcg gtcctcgtcg cgatcgcgtt ctccgcgctc ttcgtgttcc tccagtggcc
      481 gctcctgatc taccacgtca ccgacccgaa cgtctggacc acggtgttcg aaacgctcgc
      541 gctctccggc gcagcgtgga ccctggtgac gtccaccgcc gctcccggcg agaaacgcgt
      601 cccgcccgcc cttggacggc tggcgtacgg gctcgcgctc cccgcattcg gcatcctcca
      661 cttcaagtac gcccacttcg tcggcacgct catcccggcg tggatccccc tgcgcacggc
      721 gtgggccgta ttcatcggca tctgcttcgt cgccgccggc ctcgccatcg tcacccgcat
      781 ccgcgcccgc ctcgcggcca cgctcctcgg cgcgatgttc ggctcctggg tggtgctgct
      841 ccacatcccg ctcgtcatca cgtcgcacgg cgccggcggg aaatggacca gcctcttcgt
      901 cgcgacgatg atgtgcggcg gggcgtggat ggtgggaggg caggacgcat ttggcgcatg
      961 aacagctgcg tgaaaggccg gatgaactgc cgcgtgggcg ggtgaggcgc aggaggtgca
     1021 tagcgacatc gcactacgca ccacgatcgg cttgagatcc ttcccttcgg caagctcagg
     1081 gcaggcgctg cgcccaggat gacgcgcgtg accgggtgtc gggtcgccgc aggcgacccc
     1141 cacgtctatc cggcgcgcta cccccagtcg tgcgaatccg ccccaccctg ccgcagcgcc
     1201 tgcaggagct cggtcggtgc ccgcttcatc ggcgtgtcct ccggccccct tcctttaaga
     1261 gcgagcggcc gcaccccttc ggccaggatg ttgacccccg gtccctccct ggaaagcttc
     1321 cccgcgatcc acaggaacgg ctccccccgc acggtggtcc ggtcgcgctc gtagacgtcg
     1381 ggcgtcagga tcgcgttcat catccccgcc tcgtcctcca gcagcacgaa gacgaacccc
     1441 ttggcggtcc ccggccgctg ccgcgccacg accatccccg ccacctccac cttcgacccg
     1501 tgcctgagtt ccttcaaccg gtcgctccgc accacataag gagggagcgc cccgcggaag
     1561 agcgcgaacg ggtgcgtggt cggcgcaaag ccgagcgtcg cgtactccgc caccaggcgc
     1621 tcgccggcgt cgagctcgcc gaagcggatg tgcgcgtgcg gatggtcgag cgccagctcc
     1681 atctgccgtc gccgggtcgg cccgtcggtc cccggctcgg tgggcgagag ccagagcccc
     1741 acctgccaga tcaggtcgcg ccgcgtcagc ccgaagtggt cgcacccgcc cacccacacc
     1801 aggtgctcga tggcggcgcg cttgagctcg gccggcgccc gccggacgaa gtcgcccacg
     1861 ctccggtagg gcccgttccg ctcccgctcg agcaccacgg cggtggcggt ctcctcgttc
     1921 catccgcgca cgaagccgag ccccacgcgc acgctgccgc cttcgcggac gcaccagacg
     1981 tcgctccgct cgaggtcggg gaggaggatc tccaccccgc cgcggcgcgc atcgcgggtc
     2041 agggcgtcga gggagtagaa ccccatcggc tggttgttga agagcgcggt gtagtactcc
     2101 accgggtggt agtgccgaag ccacgccgac tggtacgcca ggagtgcgaa cgccgccgcg
     2161 tgcgccttgg gaaagccgaa ctcggagaac gcggtgacgt gcccgaacac ctgctccgcc
     2221 acctcgtcgc tcactccctt ggcccgcgcg ccggagagga actcgacccg gaactgctcc
     2281 atcatctcct tgctccgtcg ccggctcatc gcccgccgga gcgcctcggc ctgcccggcg
     2341 gtgaacccgg ccatcgcctg gcagacctcg agcacctggt cctggtagag gatgacgccc
     2401 agggtctcgt cgagtgcgtc gcggagcagt ggatgctcca ccggtggctg gtaggggcgc
     2461 cccgccgcct cggccacgcg ctgcgcctcg cgccgcttca cgtacggatt gaccgccccg
     2521 cccaccaccg gccccggccg cacgatggcc acctccacag ccaggtcggc gatgttccgc
     2581 ggccgggtgc ggcggatgac ctggatctgc gcccggctct cgatctggaa g
//
LOCUS       DASUAP010000325         8650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_11735, whole genome shotgun sequence.
ACCESSION   DASUAP010000325 DASUAP010000000
VERSION     DASUAP010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11735"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..2619
                     /gene="gyrA"
                     /locus_tag="VFU45_08850"
     CDS             139..2619
                     /gene="gyrA"
                     /locus_tag="VFU45_08850"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5359215.1"
                     /translation="MNDIPAISLSDETQRRYLNYALSVITARALPDVRDGLKPVQRRI
                     VFDMFHDLRLLPDARPRKCAAVVGDVLGKYHPHGDSSVYDALVRMVQDFSLRYPLIDG
                     QGNFGSIDGDPAAAYRYTEARLTRIATTMLEDIDKNTVDFQANFDDRLEEPTVLPSKL
                     PNLLVNGSSGIAVGMATNIPPHNLKEVAKAVELLVDQPDATIADLRKVIKGPDFPTGG
                     FIYGREGIKDAYETGRGRVVMRARAQIEEKESSGKSQIVVTEIPYQVNRENLVRSIAE
                     LVNAKKIDGISGVNDESDKDGMRVVIQLKRDAIPHVVLNQLYKHTQMQATFGVIMLAL
                     DRGMPKVMNLMELVQRYIEHWMTVIVRRTRFELEAAQAREHILEGLKIAVDNIDEVIK
                     IIRGSETTEDADQKLRKRFKLSEKQSDAILNMRLAKLTGLEIEKLEAELAEVRALIAD
                     LQGILASTARRAEILKSELREVADKFGDERRTEILSDQGEFTVEDLIAEEDMVITISH
                     TGYIKRIPVSAYRKQRRGGRGLTGTDLKENDWVEHLFIASTHDYLMFFTNQGQVYWLK
                     VHEIPQASRAARGKPVVSCIAVRPEEQVAALVPVREFSDQQWLVFATRQGTVKKTRLS
                     EYGNVRANGIRAINIDEGDELIDVQVCEANNDIILATRAGMSIRFHQGDVREMGRATS
                     GVKGIQLDDDDRVIGMVVVRRDATLLVVSEKGFGKRSELSEYRVQKRSGKGIITFKVT
                     DKTGSIVALKEVIPQDELMMITHLGVIIRVPVDGIRVIGRNTQGVKVMNLDAGDNLQA
                     VARVVKEDETGQEDTAEEPVPAGDDTGE"
     gene            2600..3553
                     /locus_tag="VFU45_08855"
     CDS             2600..3553
                     /locus_tag="VFU45_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEU5359216.1"
                     /translation="MTTPASEVTPASIRAAARSLEGVAVRSRLLPLTRPSGGRFFLKC
                     EQDQPIGAFKIRGAWTALSRLAPESRVRGVVTHSSGNHAQAIAFAAHRLGMPAVIVMP
                     QDAPAIKIAGVRKYGGEIVFVQDRSERDPRCRAIAAERGMTMVPPYEHADVIAGQGTI
                     AIELLEQCPELRTVLVPVGGGGLLAGVTAWLRDAAPHVRIIGIEPAGAPKLGAALDAG
                     HPTAVASPRSIADGLLPAAIGDLPFSVIGGTVKEAVQIPDDAIRAAVRFLYRDCGLAV
                     EPSGAITTAALLSGAVVAEGPVVAIASGGNIDPDLLQSLVT"
     gene            3554..4984
                     /locus_tag="VFU45_08860"
     CDS             3554..4984
                     /locus_tag="VFU45_08860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5359217.1"
                     /translation="MGLAPKILVADDDQTLSRTLSWILRENGYDVVAIPGGEHLFEHL
                     SADQFDLLLLDIMMPRVNGLDLLQKVREDGRWRDLPVLMISSMPPEDATVKALGLGAQ
                     DFIPKPFRVRELLARVKAHLRVGRELNQARTEARSRSEMVDILQEVASSLTPEEIFQV
                     LVRRVAQGLRISRCSIILGEPGESQGTVVAAFENPTLRNLRVDLGRYPEIRHALDTGE
                     LVLAQDVATDGLYDQIREEWQQEGRAIPVRSAIAMRFDLKGHPAGVFFLRTTDEDVPL
                     NQQDVQFANQIISAAVTSLERAYDLQRAVTGQEQLRHLASTDPLTGVLNRRALGEKIE
                     QEMDRAARYASLLTCLMVDIDNFKQINDTYGHLVGDRILKQLATLLKRELRSPDTVAR
                     YGGEEFVVLLPETTATESRNFADRVLKRVANYDFGEPGRPVRVTISIGLASYPDERVT
                     DGESLLRLADHHLYRAKSEGRNRVRD"
     gene            5113..6099
                     /locus_tag="VFU45_08865"
     CDS             5113..6099
                     /locus_tag="VFU45_08865"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5359218.1"
                     /translation="MPRIRVSADDAPRPSAEDSQRLGSLTGTTLDDRYRIGRKVGEGG
                     MSWVYQAEDTQSGRTVAIKLLPPRLARDRSAVERLRREAEIADRFEHPNVCPILAFGY
                     TGDGALYLVMPFLEGETLSDYELRTRAVSVADGVRILSQVCRGLQHAHDLGVIHRDLK
                     PENVMLVTEDAGLGTRDSQRPRPASRAVVMDFGLAKQRRAGAEVVKLTQTGIVLGTPE
                     FMSPEQIRGRPLDGRSDIYAVGVLAFELFTGELPFTGKNAQETMMARLRGLPRRLRDL
                     RPDLPARLESVIARALALAPEERYQSMNEFADALDRLAAGGPISRLFRRRQQ"
     gene            6127..6936
                     /locus_tag="VFU45_08870"
     CDS             6127..6936
                     /locus_tag="VFU45_08870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5359219.1"
                     /translation="MKLLTRRAGIASHLAVAAIAAGVGALGYWALLQDHVRDRVTTHG
                     EWRWIPRGADSVRAYVAYPERADRAPAVIVIHEIFGLTDWEPTVADKLAGRGYVAIVP
                     DLLSRRFGMSPADPDSGRKLVAMLEPDSITADLDAAYAWLNEQPAVRPGDVATIGFCW
                     GGGQSFRYATNNPKLRAVVVCYGPAPDSAGLARIKAPILGVYGEQDARINQDLPRVEA
                     FMRNSGKRYTAEVYPGTGHGFLKPGRVGSDGPQVDRAWTRILNFYRENLGR"
     gene            6948..7235
                     /locus_tag="VFU45_08875"
     CDS             6948..7235
                     /locus_tag="VFU45_08875"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEU5359220.1"
                     /translation="MLLRFRPEVGDETLTAIRDGLLALGHLVPEVRRITFGPNLAPER
                     EAWPWVIVVDVDDLPALERYAIHPAHQRVLTELVRPNLAGRLAADVEVGGK"
     gene            7358..8095
                     /locus_tag="VFU45_08880"
     CDS             7358..8095
                     /locus_tag="VFU45_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359221.1"
                     /translation="MLTLLTACTLPPLRGKAQVGYDPYAIVAADGNGGTDLYAIIGAG
                     NDVVRVTFTPVGEEAPVLSPDGGMVAFLRTPRPGGSGVPQVWVLNLLRGMERELKFPA
                     GPAPAVRRLGWSPDSRRIYAETGAGLWQWPIPPGDSVASVVPVEGRAAADSGLRVLLG
                     DPPFASVVACDSAGPALCVLTRDSVRQVLARNASAAARWGSDSVAWIVEGKIAVRPLG
                     GGETRIIELKENALNPEALTYFPGTRR"
     gene            complement(8052..>8650)
                     /locus_tag="VFU45_08885"
     CDS             complement(8052..>8650)
                     /locus_tag="VFU45_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5359222.1"
                     /translation="RQRLLRAAIELFTAQGFRATTTPLIAARAQLAEGTIYRHFRGKD
                     VLFNETRRRSVEWATGVVRDLSAERQPPRETLTRIGRRLAEGAQRDAAVVRMALAAPN
                     DGPAPDEACRLAVQAFRGALELIVAGGKSDGVIRPGPAELWCGIWLAVVRYCTERVAA
                     GEWAPDHPQVTLTLDAAWDAIAARATAASPESTSAPPG"
BASE COUNT         1373 a         3020 c         2984 g         1273 t
ORIGIN      
        1 caagcacgtt cgcccgaacg cgtaagcgcc cgccgagaga ctcgcctccc gctcgacccc
       61 gcttgacttc cgtggtcgac cttctatttt gacgcgcaaa ccgtgtcctc cgatcagctg
      121 acactgttcg aaagccgcat gaacgacatc ccagccatct ccctctccga cgagacccaa
      181 cgccgttacc tcaactatgc gctctcggtg atcaccgcgc gcgcgctgcc cgacgtgcgc
      241 gacgggctga agccggtgca gcggcggatc gtcttcgaca tgttccacga tctgcgcctt
      301 ctgcccgacg cgcgcccgcg caagtgcgcg gcggtggtcg gcgacgtgct cggcaagtat
      361 cacccgcacg gcgacagctc ggtctacgac gcgctggtgc gcatggtgca ggacttctcg
      421 ctccgctatc cgctgatcga cgggcagggg aacttcggct ccattgacgg cgatcccgcc
      481 gcggcgtacc gctacaccga ggcccgcctc acccgcatcg ccaccacgat gctggaagac
      541 atcgacaaga acacggtgga cttccaggcc aacttcgacg accggctgga ggagccgacg
      601 gtcctgccgt ccaagctccc caacctgctg gtcaacggct ccagcggcat cgcggtgggc
      661 atggccacca acatcccgcc ccacaacctg aaggaagtgg cgaaggcggt ggagctgctg
      721 gtggaccagc ccgacgccac catcgcggac ctccgcaagg tgatcaaggg ccccgatttc
      781 ccgaccggcg ggttcatcta cggccgggag gggatcaagg acgcctacga gaccggccgc
      841 ggccgggtgg tgatgcgggc gcgggcccag atcgaggaga aggagtcgag cgggaagtcg
      901 cagatcgtgg tgaccgagat cccctaccag gtgaaccgcg agaacctggt gcgctccatc
      961 gccgagctgg tcaacgccaa gaagatcgac ggcatcagcg gggtgaacga cgagtccgac
     1021 aaggacggca tgcgggtggt gatccagctc aagcgggacg ccatccccca tgtggtgctg
     1081 aaccagctct acaagcacac ccagatgcag gcgaccttcg gcgtgatcat gctggcgctc
     1141 gatcgcggca tgccgaaggt catgaacctg atggagctgg tgcagcgcta catcgagcac
     1201 tggatgacgg tgatcgtgcg ccggacccgg ttcgagctgg aggcggcgca ggcccgggag
     1261 cacatcctcg agggcctcaa gatcgccgtc gacaacatcg acgaggtgat caagatcatc
     1321 cgcggctccg agacgacgga ggacgcggac cagaagctcc gcaagcgctt caagctctcc
     1381 gagaagcaga gcgacgccat cctcaacatg cgcctcgcca agctgaccgg gctggagatc
     1441 gagaagctgg aggccgagct ggcggaagtg cgcgcgctga tcgccgacct ccagggcatc
     1501 ctggcgagca ccgcccgccg ggcggagatc ctcaagagcg agctgcggga ggtggcggac
     1561 aagttcggcg acgagcgccg caccgagatc ctctccgacc agggcgagtt cacggtggag
     1621 gacctgatcg ccgaggagga catggtcatc accatctccc acaccggcta catcaagcgc
     1681 attcccgtct ccgcctaccg gaagcagcgg cggggcgggc gcggcctcac cggcaccgac
     1741 ctcaaggaga acgactgggt cgagcacctg ttcatcgcca gcacccacga ctacctgatg
     1801 ttcttcacca accaggggca ggtgtactgg ctcaaggtgc acgagatccc gcaggcctcc
     1861 cgcgcggcgc gcggcaagcc ggtggtgagc tgcatcgccg tgcggcccga ggaacaggtg
     1921 gcggcactgg tgccggtgcg cgagttcagc gaccagcagt ggctggtctt cgccacccgg
     1981 cagggcaccg tcaagaagac ccgcctctcc gagtacggca acgtgcgcgc caatggcatc
     2041 cgcgccatca acatcgacga gggcgacgag ctcattgacg tgcaggtctg cgaggcgaac
     2101 aacgacatca tcctcgcgac ccgcgccggc atgagcatcc gcttccacca gggcgacgtg
     2161 cgcgagatgg gccgggccac cagcggggtc aagggcatcc agctcgacga cgacgaccgg
     2221 gtgatcggca tggtggtggt gcggcgcgac gccacgctgc tggtggtgag cgagaagggc
     2281 ttcggcaagc gctccgaact ctcggaatac cgggtacaga agcggagcgg caaggggatc
     2341 atcaccttca aggtcaccga caagaccggc tcgatcgtgg cgctcaagga ggtgatcccg
     2401 caggacgagc tcatgatgat cacccacctg ggtgtcatca tccgggtgcc ggtggacggc
     2461 atccgcgtga tcgggcggaa cacgcagggc gtcaaggtca tgaacctcga cgccggcgac
     2521 aacctccagg ccgtcgcccg cgtggtgaag gaagacgaga ccggccagga ggacacggcc
     2581 gaagagcccg tgccggccgg tgacgacacc ggcgagtgag gtaactcccg cttccatccg
     2641 cgccgcggcc cgttcgctcg agggggtcgc ggtgcggagc cggctgctgc cgctcacccg
     2701 tccctcgggc ggccgcttct tcctcaagtg cgagcaggac cagccgatcg gcgccttcaa
     2761 gatccggggc gcctggaccg cgctctcgcg ccttgcgccc gagtcccgcg tccgcggtgt
     2821 cgtcacccat tccagcggca accacgccca ggccatcgcc ttcgccgccc accggctcgg
     2881 catgcccgcc gtgatcgtca tgccgcagga tgcaccggcc atcaagatcg ccggggtgcg
     2941 gaagtacggc ggcgagatcg tcttcgtgca ggaccggagc gagcgggatc cgcgctgccg
     3001 ggcgatcgcc gccgaacgcg ggatgacgat ggtgccgccc tatgagcatg ccgacgtgat
     3061 cgcggggcag ggcaccatcg cgatcgagct gctggagcag tgcccggagc tccggacggt
     3121 gctggtgccg gtgggcggcg gcggactgct ggccggggtc accgcctggc tgcgtgatgc
     3181 ggcgcctcac gtgcggatca tcggcatcga gccggccggc gcgcccaagc tgggtgcggc
     3241 gctcgatgcc ggccacccga cggcggtggc ctccccccgc agcattgcgg atggactcct
     3301 ccccgcggcc atcggagact tgcccttttc ggtcatcggc ggcaccgtga aggaggccgt
     3361 acagattccc gacgatgcca tccgcgcggc ggtccgcttc ctctaccggg actgcgggct
     3421 ggccgtcgag ccgtcgggcg ccatcacgac cgcggccctg ctgtccggtg cggtggtggc
     3481 ggaaggcccc gtggtggcca tcgcgagcgg cggcaacatt gaccccgacc ttctccagtc
     3541 actggtgacc tagatggggc tcgcgcccaa gatcctcgtc gccgacgacg accagaccct
     3601 ctcccgcacc ctctcgtgga tcctccggga gaacggctac gacgtggtgg ccattcccgg
     3661 cggcgagcac ctcttcgagc acctctccgc cgaccagttc gacctcctgc tgctcgacat
     3721 catgatgccg agggtcaacg gcctggacct gctgcagaag gtgcgcgagg acgggcggtg
     3781 gcgggacctg ccggtgctga tgatctcctc gatgccgccc gaggatgcca ccgtcaaggc
     3841 gctgggcctc ggcgcgcagg acttcattcc caagccgttc cgcgtgcgcg agctgctggc
     3901 gcgggtcaag gcgcatctcc gcgtcgggcg cgagctcaac caggcgcgca ccgaggcacg
     3961 ctcgcgctcg gagatggtgg acatcctcca ggaagtggcg tccagcctga cgccggaaga
     4021 gatcttccag gtgctggtgc gccgggtggc gcagggcctt cggatctccc gctgctccat
     4081 catcctcggc gagccgggcg aatcgcaggg cacggtggtg gcggcgttcg agaaccccac
     4141 gctccggaac ctccgggtgg acctgggccg ctatcccgag atccgccacg cgctcgacac
     4201 cggcgagctg gtgctggcgc aggatgtcgc cactgacgga ctgtacgacc agatccgcga
     4261 ggaatggcag caggagggac gggcgattcc ggtccggtcc gccatcgcca tgcgcttcga
     4321 cctcaagggc catcccgccg gcgtcttctt cctccgtacc accgacgagg acgtgccgct
     4381 caaccagcag gatgtgcagt tcgccaacca gatcatctcg gcggcggtga cgtcgctgga
     4441 gcgggcctac gatctccagc gcgccgtcac cgggcaggag cagctgcggc acctggcatc
     4501 caccgatccg ctcaccggcg tgctcaaccg ccgcgcgctg ggcgagaaga tcgagcagga
     4561 gatggaccgc gccgcgcggt atgcgtcgct cctcacctgt ctcatggtgg acatcgacaa
     4621 cttcaagcag atcaacgaca cctacggtca cctggtgggc gaccggatcc tgaagcagct
     4681 ggcgacgctg ctcaagcggg agctgcgctc ccccgacacc gtggcgcgct acggcggcga
     4741 ggagttcgtg gtgctgctgc cggaaaccac cgccaccgag agccggaact ttgccgaccg
     4801 cgtgctcaag cgggtggcca actacgactt cggcgagccg gggcggccgg tgcgggtcac
     4861 catcagcatc gggctcgcgt cctatcccga cgagcgcgtc accgacggcg agtcgctgct
     4921 gcgcctcgcc gaccatcacc tctaccgggc caagagtgaa ggccggaacc gcgtgcgcga
     4981 ttgaccaacc ggcgttgccc ccgttgtcag gcgcaattcg atgcgcccgc ccgctactgc
     5041 cggcgctgcg gtacccccct tgtcgaggtt gctccaccgg cgccgcgttc cgggtcgccc
     5101 cacctggccg atgtgccccg gattcgcgtc tccgccgacg atgctccccg cccgagcgcc
     5161 gaggactccc agcgactcgg ctcgcttacc ggcaccacgc tggatgaccg ttaccgcatc
     5221 ggccgcaagg tgggcgaggg tgggatgtcg tgggtctacc aggccgagga cacgcagtcc
     5281 gggcgcaccg tggcgatcaa gctgctgccg cctcgcctgg cccgggaccg ctcggcggtg
     5341 gagcggctcc ggcgcgaggc ggagatcgcc gaccggttcg agcatccgaa cgtctgcccc
     5401 atcctcgcct tcggctacac cggggacggc gcactctacc tcgtgatgcc gttcctcgag
     5461 ggcgagacgc tctcggatta cgagctccgg acccgcgcgg tgagcgtcgc ggacggtgtg
     5521 cggatcctgt cgcaggtctg ccgcggcctg cagcatgcgc acgacctggg ggtgattcac
     5581 cgggatctga agcccgagaa tgtgatgctg gtaacggagg acgcgggact cgggactcgg
     5641 gactcgcagc gcccgcgtcc cgcgtcccgt gccgtagtaa tggacttcgg cctcgccaag
     5701 cagcggcggg ccggcgccga ggtggtgaag ctcacccaga ccgggatcgt gctggggacg
     5761 ccggagttca tgagcccgga gcagatccgt gggcgccccc tcgacgggcg gagcgacatc
     5821 tacgccgtgg gcgtgctggc gttcgagctc ttcaccggag aacttccctt caccggcaag
     5881 aacgcgcagg agaccatgat ggcccggctc cgcgggctcc cgcgccggct ccgggacctg
     5941 cggcccgacc tgcccgcgcg gctcgagtcg gtcatcgcgc gggcgctcgc gctggcgccg
     6001 gaggagcggt accagtccat gaatgaattc gccgacgccc tcgaccggct cgccgccggc
     6061 gggccgatct cccggctctt ccgccggcgc cagcaataac ggccgtacgc tgcaaggagg
     6121 atcgagatga agctgctcac caggcgggcc ggcattgcct cgcatctcgc cgtggcggcg
     6181 attgccgccg gcgtcggtgc cctgggctac tgggccctgc tgcaggatca tgtccgggac
     6241 cgggtgacca cccacggcga gtggcgctgg atcccgcgcg gcgccgactc ggtgcgcgcg
     6301 tacgtcgcct accccgagcg cgccgaccgg gcccccgccg tcatcgtcat ccacgagatc
     6361 ttcgggctca ccgactggga gccgacggtg gccgacaagc tcgccggccg cggctatgtg
     6421 gccatcgtgc ccgacctgct ttcccggcgg ttcgggatgt cacccgccga ccccgattcg
     6481 ggccgcaagc tcgtggccat gctggagccg gacagcatca ccgccgacct cgacgccgcc
     6541 tatgcctggc tcaacgaaca gccggcggtg cgccccggtg acgtggccac catcggcttc
     6601 tgctggggcg gcggccagag cttccgctac gccaccaaca acccgaagct ccgcgcggtg
     6661 gtggtctgct atggccccgc accggacagc gccggcctgg cccgcatcaa ggcaccgatc
     6721 ctcggcgtct acggcgaaca ggatgcccgc atcaaccagg accttccgcg ggtggaagcg
     6781 ttcatgcgga attcgggcaa gcgctacacc gcggaggtct accccggcac cgggcacggg
     6841 ttcctgaagc ccgggcgggt gggatccgac gggccgcagg tggaccgcgc gtggacccgc
     6901 atcctcaact tctaccgcga gaacctcggc cgctgatcac tcatgtcgtg ctgctccggt
     6961 ttcgtcccga ggtcggggac gagacgctga ccgccatccg ggacgggctc ctcgcgctgg
     7021 gccatctcgt gcccgaggtg cgccgcatca ccttcggccc gaacctcgcg cccgagcgcg
     7081 aggcctggcc gtgggtgatc gtggttgacg tggacgacct gccggcgctg gagcgctatg
     7141 ccatccatcc cgcgcatcag cgtgtgctga ccgagctggt ccggccgaac ctcgcgggcc
     7201 ggctggcggc ggatgtggaa gtgggtggga agtgaagagc gagagacgag catttgtcac
     7261 tctgaggccg cgacgcggcc gaagaggcgg agagcagcct gaatttcggc cccttcgctt
     7321 cgctcagggt gacatctccc tcgtctctgg catcctgttg ctcacactgc tgacagcctg
     7381 cacgctgccg ccgctccggg gcaaggcgca ggtggggtac gatccctacg ccatcgtggc
     7441 ggcggacggc aacggcggga ccgatctgta cgccatcatc ggcgccggga atgacgtcgt
     7501 gcgggtcaca ttcacgccgg tgggggagga ggcgccggtg ctgtcgcccg acggggggat
     7561 ggtggcattc ctccggacgc cgcggcccgg cgggagcggc gtgccgcagg tgtgggtgct
     7621 gaacctgctc cgcggcatgg agcgggagct caagtttccg gccggtcccg cgccagcggt
     7681 ccgccggctg ggctggtcgc cggacagccg ccggatctac gccgagaccg gcgcggggct
     7741 ctggcagtgg ccgatcccgc caggcgattc ggtggcgagc gtagtgccgg tggaagggcg
     7801 cgccgcggcc gattcgggac tgcgggtgct gctgggcgat ccgcccttcg cgtcggtcgt
     7861 cgcctgcgac tcggcggggc cggccctctg cgtgctgacc cgggacagcg tgcggcaggt
     7921 gctggccagg aatgcgtcag cggccgcgcg ctggggcagc gattcggtcg cctggatcgt
     7981 ggagggaaag atcgccgtgc gcccgctcgg cggcggcgag acccggatca tcgaactcaa
     8041 ggagaacgcg ctcaacccgg aggcgctgac gtactttccg gggacgcggc ggtagcgcga
     8101 gccgcgatcg catcccacgc cgcatccagg gtgagcgtca cctgcgggtg atcgggcgcc
     8161 cattcccctg cggccacccg ttcggtgcaa tagcgcacca ccgccagcca gattccgcac
     8221 cagagctcgg ccggtcccgg ccggatcact ccgtcagact tgcctccggc cacaatcagc
     8281 tcgagggcgc cgcggaacgc ctgcactgcc agtcggcacg cctcatcggg tgccggtccg
     8341 tcattcggtg ccgcgagcgc catccgcacc acggcggcgt cccgctgcgc gccttccgcg
     8401 agccgccggc caatccgcgt gagtgtttcc cggggcggtt gccgctccgc cgagagatcc
     8461 cggaccacac ccgtggccca ttccaccgac cggcgccgcg tctcgttgaa gagcacgtcc
     8521 ttgccccgga agtgccggta gatggtgccc tccgcgagct gcgcgcgggc ggcgatcaag
     8581 ggtgtggtgg tggcgcggaa cccctgggcg gtgaagagct cgatggcggc ccggagcagc
     8641 cgctggcggg
//
LOCUS       DASUAP010000326        10660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_120468, whole genome shotgun sequence.
ACCESSION   DASUAP010000326 DASUAP010000000
VERSION     DASUAP010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120468"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..2614)
                     /gene="lon"
                     /locus_tag="VFU45_08890"
     CDS             complement(110..2614)
                     /gene="lon"
                     /locus_tag="VFU45_08890"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU5359223.1"
                     /translation="MSDRLTLPVLPLREVVLFPGVTAPIGAGRPATLKAIEAAMATPD
                     RLVFAVSQRQNTEAVTPDNLHTIGTIARIGQLQRGLSGMQLLLNGDRRGIAMHIVEKD
                     GFLQAVVREAEEMPPLNPDDPAFVALYREARAKAAELGQKSGLPEEVVQQVLAGVSEP
                     GRFADLVAGYIDIAPAQRQMLLETLSVEERLRRVLVHVQRQVSVLEAQEDIKSQVQEE
                     LGERQREMFLREQLKQIRRELGEDEEGDDVDELRTKLAALTLPDVVRKEVDRELNRLA
                     RIGRESMESQVIRTFLETIAELPWSERSAEHLDLVEASKILDEDHYALKDVKDRVLEF
                     LAVRQLRHAAEPAPGAPTAAGGAEPEAVEPPSPAPVEKVPGNDDDKRAKGPILLFVGP
                     PGVGKTSVAKSIARAMGRKYVRISLGGARDEADIRGHRRTYVGAMPGRIIQGMKQAGT
                     RNPVFLLDEVDKLGVSFQGDPASALLEVLDPAQNDSFTDHYLGVPFDLSEVLFICTAN
                     FIQNLPAPLLDRMEVVEFTGYTEREKLAIARQYLVPRQFEEHGLTSAQLSVTDAAVSE
                     VISAYTREAGVRQLERELGKLARKVARRIATKEAEVVTVDRADVAELLGRPRVHPERA
                     ARSDQVGVATGMYYTPVGGDIMFVEASTMRGKGELVLTGQLGDVMKESGRAAWTYARS
                     HAAGLHIKEEMFDRDVHIHVPAGAIPKDGPSAGVTMATALVSALSNRPARHDIAMTGE
                     VTLTGRVLPIGGVKEKVLGAVRSGITQIVLPKENVRDLEDLPKEVRDTIEVFPVQELG
                     EVLAITLRGASFREGRLLFGNENPQDVAPLSQGFPH"
     gene            2892..4817
                     /locus_tag="VFU45_08895"
     CDS             2892..4817
                     /locus_tag="VFU45_08895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5359224.1"
                     /translation="MTDELQQPLAQRLARALGATYTIEGEIGRGGMGVVYRARDERLQ
                     RRVAIKVLPPELAFQDDIRARFMREAQTAARLSHPNIVPIHDVGESDGLTYFIMGYVD
                     GESLAGRIKRRGQLPAEDARRIMKETADALSAAHALSIIHRDIKPDNILLDGTRARVM
                     VTDFGIAKALSGGGGATLTSAGVAIGTPSYMSPEQAAGEKDIDGRSDLYSLGICGYQM
                     LTGQPPFTAPTVAGVLMKQITEPAPLVSDARPDVPEDLVLAVARCLEKDPENRWPTAD
                     ALRRSLESRTTGTYRPTGTSFRAAQRQATRPSQTARRAPGTGTRGTSGLQRPDVPRSP
                     RPNLPVRGEARRREQRLDRWNWDESRGRDRELVRDTGEPEIVQRVRQKFARWAAPTAG
                     MVLINIATGFHSPWSLVVGAIWAFGSGLISDYSKLWHAGYSWKDVLQRPPAPDAIEAR
                     GTSAAGGKFPRMLPPPSPAEFGMHSARMRQAHGDRAAIYKLLDKMPPADRQMLPDVAQ
                     TVDGLYARAQELGRTLQAMDTGFGPNDAGRIDLRLAALQQEPAGEERDRQQALLEKQK
                     AALADIQSRRSQIEGHFESCVLAIQNVRYDLLRLRQAGLDAVVNDLTHATQAARALSR
                     DVDVAIAAAGELREALE"
     gene            complement(4912..7038)
                     /locus_tag="VFU45_08900"
     CDS             complement(4912..7038)
                     /locus_tag="VFU45_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5359225.1"
                     /translation="MATRTRPQPKTAKSLSKADLLDIYRVMLLSRRIDDKEIQLKRQN
                     KIFFQISGAGHEAVLAAAAKVFRPAYDWFYTYYRDRALCLGLGQTATDQLLSAVAAAD
                     DPNSGGRQMPSHWGAKDLNIVSSSSPTGTQFLQAVGCAEAWLRAEQNPALPDRDAMVR
                     KDEVVFCSTGDGTTSEGEFWEALSSASNLKLPVVFVVEDNGYAISVPVEVNTPGGSIS
                     RIVTGFPGLYVEECDGCDPVASFETLTRAVDYARQRKGPALVHAHVIRPYSHSLSDDE
                     VFYRPTAEREAEARKDPLVRFPKYLLDQGLATQAELDAIKAKVEEEVEVASQTALAAP
                     QPATDTVYRYVYSPDVDPTSERFDTEDDPKFSGEPTTMVDLLNGCMKDEMARDPRILV
                     FGEDVADCSRDENLGQVKGKGGVFKVTWGLQRAYGSNRVYNSPLAEANIIGRAIGLAT
                     RGFKPVVEIQFFDYIWPAYQQLRNELATMRWRSNNAFSAPVVVRVTYGGYLKGGAIYH
                     SQTGAAVFTGVPGLRVVCPSTALDANGLLRTAIRSEDPVIFLEHKHLYRQTYNKAPNP
                     GPNFTIPFGKAKVVRPGRHLTVVTYGAVVQRALVAAKAAEEQHGLEVEVIDLRTLSPV
                     DYETIYASVRKTNRVIVAYEDSMSWGYGAEIAARIADAAFPWLDAPVKRVAATDTFVA
                     YAPELEDVILPQSEHLLKAYQELAAF"
     gene            complement(7054..8742)
                     /locus_tag="VFU45_08905"
     CDS             complement(7054..8742)
                     /locus_tag="VFU45_08905"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5359226.1"
                     /translation="MHRRSAFMLLALTALLMHGSSAAAQATLVIRGRIWTGDPAHPWA
                     SALAVAGDTIAAIGDRASVARLIGPTTEIVDAPGLVTPGFQDGHTHFIDGGFQLASLD
                     LRTVATPAEFIRRIRDFVRHVPKGRWIIGGDWDHTLWPGQPLPRHEWIDSVTPDNPVF
                     LERLDGHEALANAAAMRAAGITRATAAPPGGEIGHDPRTGELTGIFKDQALRMVYAVV
                     PDPTPEERDSALVRALAYAASLGVTGTSSMWASFADLQSFRRVERAGRLTMRAALYLP
                     LENWHAVADTIAADGAGDDWVRIGGLKEFMDGSAGSRTAFFAQPYADSAGYRGLLRNS
                     PADMARWIGAADSVHLQVAVHAIGDSANAILLGIYDSLTTAHGPRDRRFRIEHAQHLR
                     PQDIARFGRLGVIASVQPYHLVDDGRWIQHRIGPVRIHSTYPFRTLLDTHAELAFGSD
                     WTVATLDPIQGIAAAVTRRTSDGKNPGGWVPEQKITLSEALRAYTWGNAYANFTEAHR
                     GTLAPGKWADITVLDKDLFTLPPAELGTARAVLTVVGGKVVYRRPGALLAHPRP"
     gene            complement(8762..9013)
                     /locus_tag="VFU45_08910"
     CDS             complement(8762..9013)
                     /locus_tag="VFU45_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359227.1"
                     /translation="MRPRDHILGNLDTIYRDSYAAAKAEGQTRRMEELDAAYQRDQLM
                     MEVFLDVRDLLAARPAAPSQGPGTALQQLDALRRITKLR"
     gene            9145..9477
                     /locus_tag="VFU45_08915"
     CDS             9145..9477
                     /locus_tag="VFU45_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359228.1"
                     /translation="MAKLMKLFLTLLLLSGLAAAGSYGVAYLAQGKLVGAQPLELGEQ
                     QTRFATEGIAGLPGAPKAWVFTYGPTKIPGMKLAEIYVSPLGAVIGTKPKDFSARLQA
                     YRAAQSPD"
     gene            9487..9810
                     /locus_tag="VFU45_08920"
     CDS             9487..9810
                     /locus_tag="VFU45_08920"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEU5359229.1"
                     /translation="MAFRDAQTRVDAWISQFEEGYFEPLTNLARLTEEVGELARELNH
                     RYGQKTKKRDEPEGDLPLELADILFVLICLANREGVDLDQAFDRMMQKIEGRDRDRWT
                     RKPPA"
     gene            complement(9818..>10660)
                     /gene="rsgA"
                     /locus_tag="VFU45_08925"
     CDS             complement(9818..>10660)
                     /gene="rsgA"
                     /locus_tag="VFU45_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU5359230.1"
                     /translation="GAEVRAVLRGKLKRQTPQVIVGDRIQLEDAPQGELYGIAAVEPR
                     RSIVERRVPLGRGARPVVANVDRVIVVTASIDPAPIPSLLDRLLVVAEVNDLPAVLVV
                     NKTDLDPGEALVRRYADAGYTVVPTSAKTGAGIAQLRALLVHGESVVTGPSGAGKSSL
                     LNAIGIGLVLRTGAISDRGKRGRQTTVGAVMVPFGADAFLVDTPGFSEVGLWGVEPRE
                     LAACFPEFRPLLGQCRFPDCRHVSEPGCRIAAAAAEGKVHPDRLASYRLLLEETEAEP
                     ADWE"
BASE COUNT         1584 a         3635 c         3857 g         1584 t
ORIGIN      
        1 agtggggacg tgggggacga gcgcgatccg agcgagccct tcttcccatt tccccttttc
       61 cctcttccct cttcccttct acgtcttacg tcttacgtct cactggcgtt cagtgcggga
      121 acccctgcga caggggcgcc acgtcctgcg ggttctcgtt gccgaagagg agccgtcctt
      181 cgcggaacga ggcgccgcgc agggtgatgg ccagcacctc gccgagctcc tgcaccggga
      241 agacctcgat cgtgtcgcgc acctccttgg ggaggtcttc gaggtcgcgc acgttctcct
      301 tcggcagcac gatctgggtg atccccgacc ggaccgcccc caggaccttt tccttgaccc
      361 cgccgatcgg gagcacgcga ccggtgaggg tgacctcgcc cgtcatcgcg atgtcgtgcc
      421 gggccgggcg gttggagagc gccgaaacga gcgccgtggc catcgtgacg ccggcggagg
      481 gcccgtcctt ggggatggcg cccgccggta cgtggatgtg cacgtcgcgg tcgaacatct
      541 cctccttgat atggaggccc gccgcatggg agcgggcgta ggtccacgcc gcgcgccccg
      601 actccttcat cacgtcgccg agctggccgg tgaggacgag ctcgcccttg ccgcgcatcg
      661 tggacgcctc gacgaacatg atgtcgccgc cgaccggcgt gtagtacatc ccggtggcca
      721 cgccgacctg gtcgctccgc gcggcccgtt cggggtgcac ccgcgggcgc ccgaggagct
      781 cggccacgtc ggcgcggtcg accgtcacga cctcggcctc cttggtggcg atgcgccgtg
      841 cgaccttccg ggccagcttg ccgagctcgc gctcgagctg ccggacgccg gcctcgcggg
      901 tgtaggcgct gatcacctcg ctcacggcgg cgtcggtcac cgagagctgc gccgacgtga
      961 ggccgtgctc ctcgaactgc cgcggcacca ggtactggcg cgcgatggcg agcttctccc
     1021 gctcggtgta gccggtgaac tcgaccactt ccatccggtc gagcagcggg gccggcaggt
     1081 tctggatgaa gttcgccgtg cagatgaaca gcacctcgct caggtcgaac ggcacgccca
     1141 ggtaatggtc ggtgaagctg tcgttctggg ccgggtcgag cacctcgagg agcgcgctcg
     1201 ccgggtcgcc ctggaaggag acgccgagct tgtcgacctc gtcgaggagg aagacggggt
     1261 tgcgggtgcc ggcctgcttc atcccctgga tgatccggcc gggcatcgca ccgacgtagg
     1321 tgcggcggtg gccgcggatg tcggcctcgt cgcgcgcgcc gccaagcgag atgcggacgt
     1381 acttccgccc catcgcccgg gcgatcgact tcgcgaccga cgtcttgccg acgccgggcg
     1441 ggcccacgaa gaggaggatc gggcccttgg cgcgcttgtc gtcgtcgttg ccgggcacct
     1501 tctcgaccgg ggccggtgac ggcggctcga ccgcttcggg ctcggctccg ccggcggccg
     1561 tcggcgcacc gggggccggc tccgcggcgt ggcgaagttg ccgcacggcc aggaactcga
     1621 gcacccggtc cttcacgtcc ttgagggcgt agtggtcctc gtcgaggatc ttggaggcct
     1681 cgaccaggtc gaggtgctcc gcgctccgct cggaccaggg cagttcggcg atggtctcga
     1741 ggaaggtgcg aatgacctgc gactccatcg actcacgccc gatgcgcgcg agccggttga
     1801 gctcccggtc cacctccttg cgcaccacat ccggcagcgt gagcgcggcc agcttggtgc
     1861 ggagctcgtc gacatcgtcg ccttcctcgt cctcgccaag ctcgcggcgg atctgcttga
     1921 gctgctcgcg gaggaacatc tcccgctggc gctcaccgag ctcctcctgc acctgggact
     1981 tgatgtcctc ctgcgcttcc agcacgctca cctgccgctg gacgtgcacc aggacgcggc
     2041 ggagccgctc ctcgaccgaa agggtctcga gcagcatctg acgctgggcc ggggcgatgt
     2101 cgatgtagcc ggccaccagg tcggcgaacc gacccggctc gctcacgccg gccaggacct
     2161 gctgcaccac ctcctcgggc aacccggact tctggcccaa ctcggcggcc ttggcgcgcg
     2221 cctcgcggta gagcgccacg aacgccgggt cgtcggggtt gaggggcggc atctcctccg
     2281 cctcacggac cacggcctgc aggaagccat ccttctcgac gatgtgcatc gcaatgccgc
     2341 ggcgatcgcc gttgaggagg agctgcatcc cggagaggcc gcgctgcaac tggccgatgc
     2401 gggcgatggt gccgatcgtg tggaggttgt cgggagtgac ggcctcggtg ttctggcgct
     2461 gcgacacggc aaagacgagg cggtcgggtg tggccatcgc agcctcgatg gccttgagcg
     2521 tcgcgggacg tcccgctccg atcggcgcgg tgacgcccgg gaagagcacc acctcgcgca
     2581 ggggcaggac gggaagggtc aggcggtcgg acatcgcatg tctccattgc tgggcccgcg
     2641 gaagcgcgga ccgcgatcgg gcaccgtaac gcaccccgcg tgccagccgg acgccccgat
     2701 ccagtgggcc agggatgacg aataggcgtg actcatcgat ccgccgatgc ggtagcggca
     2761 cgaccgttgc acgttcgaac gggcttcctg ccactttggc gcggccgatc gggtgccggt
     2821 ccggcggggg ccgcccggtt gccgcggcaa tgaagaggag ctaaatctat accgcttatg
     2881 atgccccccc gatgaccgac gaactccagc aaccgctggc ccagcgcctg gccagggcgc
     2941 tcggcgcgac ctacacgatc gagggcgaga tcggccgggg cggcatgggc gtggtgtatc
     3001 gcgcgcggga tgagcgcctg cagcgacggg tggccatcaa ggtgctgccg ccggagctcg
     3061 ccttccagga cgacatccgg gcgcgcttca tgcgcgaggc gcagaccgca gcacggctct
     3121 cccatccgaa catcgtcccg atccacgacg tcggggagag cgacgggctc acgtacttca
     3181 tcatgggcta cgtcgacggg gagtcgctgg cgggccgcat caagcgccgg ggccagctcc
     3241 cggcggaaga tgcgcggcgt atcatgaagg aaacggcgga cgcgctctcg gccgcgcacg
     3301 cgctttcgat catccatcgc gacatcaagc ccgacaatat cctcctcgac ggcacccggg
     3361 cgcgggtgat ggtgaccgac ttcgggatcg ccaaggcgct ctccggaggc ggcggcgcca
     3421 cgctcacgag cgcgggggtc gccatcggca cgccgagcta catgagtccg gagcaggcgg
     3481 cgggcgaaaa ggacatcgac gggcgctccg acctctacag cctgggcatc tgcggctacc
     3541 agatgctgac gggccagccg ccgttcacgg cgccgacggt cgccggcgtc ctgatgaagc
     3601 agatcaccga gccggcgccg ctcgtctcgg acgcgcgccc ggacgtgccc gaggacctgg
     3661 tgctggcggt ggcgcggtgt ctggaaaagg acccggagaa ccgctggccc accgccgacg
     3721 cgctccgccg gtcgctggag agccggacca cgggaacgta tcgtccgacc gggaccagct
     3781 tccgggccgc acaacggcag gcgacccgcc cgagccagac cgcgcgccgg gcgccgggca
     3841 ccggcacacg cggcacgtcc ggcctgcagc gccccgacgt cccgcgctcc ccgcgcccca
     3901 acctgccggt gcgcggcgaa gctcggcgcc gggaacagcg gctcgaccgc tggaactggg
     3961 atgaatcacg gggccgggac agggagctgg tgcgggacac cggcgagccc gagatcgtac
     4021 agcgggtccg ccagaagttc gctcggtggg cagcgcccac ggcaggcatg gtgctcatca
     4081 acatcgcgac cgggttccac tccccatggt cgctcgtggt aggcgcgatc tgggcattcg
     4141 ggagcggcct catcagcgac tacagcaagc tctggcatgc ggggtacagc tggaaggacg
     4201 tgctgcaacg cccgcccgca cccgacgcga tcgaggcgcg cggcacttcg gccgctgggg
     4261 gaaagttccc gcgcatgctg ccgccgccct ctccggccga gttcggcatg cactccgcgc
     4321 ggatgcggca ggcccatggc gaccgcgccg cgatctacaa actgctcgac aagatgcccc
     4381 cggccgaccg gcagatgctc cccgatgtgg cgcagaccgt ggatggcctg tacgcacggg
     4441 cgcaggaatt ggggcggacg ctccaggcga tggacacggg gttcgggccg aacgacgcgg
     4501 gccggatcga cctccggctg gccgcgctcc agcaggagcc ggccggtgag gagcgcgacc
     4561 ggcagcaggc gctcctggag aagcagaagg ccgcgctggc cgacatccag tcgcgccggt
     4621 cccagatcga ggggcacttc gagagctgcg tcctggccat ccagaacgtg cgatacgacc
     4681 tgctccggct gcggcaggca gggctcgacg cggtggtgaa cgacctgacc cacgcgaccc
     4741 aggcggcgcg ggcgctctcg cgcgacgtgg acgttgccat cgcggcggcg ggcgagctgc
     4801 gggaggcgct ggagtagtcg gtgaacggtg aacagtgaac ggtcaacggg gggccccgag
     4861 acacggagcc ccccgttcac ttttcaccgt tcactgttca ccgttcgccg actagaacgc
     4921 cgccagctcc tggtaggcct tgagcaggtg ctccgactgg ggcaggatca cgtcctccag
     4981 ctcgggcgcg taggccacga acgtgtcggt ggccgcgacc cgcttgaccg gggcatcgag
     5041 ccaggggaac gccgcatcgg cgatgcgcgc ggcgatctcg gcgccgtacc cccacgacat
     5101 cgagtcctcg taggccacga tgacgcggtt ggtcttccgc accgacgcgt aaatggtctc
     5161 gtagtcgacc ggagagaggg tccggaggtc gatgacctcg acctcgaggc cgtgctgttc
     5221 ttcggcggcc ttggccgcca cgagcgcgcg ctgcaccacc gcgccgtagg tgaccacggt
     5281 caggtgccgg ccggggcgga ccaccttggc cttcccgaac gggatcgtga agttcgggcc
     5341 cggattcggc gccttgttgt aggtctgccg gtagaggtgc ttgtgctcga ggaagatcac
     5401 cgggtcctcg gagcggatgg ccgtgcgcag gaggccgttc gcgtcgagcg ccgtgctggg
     5461 gcacacgacg cggaggcccg ggacgccggt gaacaccgcg gcgccggtct gtgagtgata
     5521 gatggcgccg cccttcaggt agccgccgta ggtgacgcgg accaccaccg gggccgagaa
     5581 ggcgttgttg gaccgccagc gcatcgtggc gagttcgttg cggagctgct ggtaggcggg
     5641 ccagatgtag tcgaagaact ggatctccac caccggcttg aagccgcggg tggcgagccc
     5701 gatggcccgg ccgatgatgt tggcctccgc cagcggcgag ttgtacaccc ggttgctgcc
     5761 gtacgcccgc tgcagccccc aggtgacctt gaagaccccg cccttcccct tgacctggcc
     5821 caggttctcg tcgcggctgc agtcggccac gtcctcgccg aagacgagga tgcggggatc
     5881 gcgcgccatc tcatccttca tgcatccgtt gagcaggtcc accatcgtgg tcggctcgcc
     5941 ggagaacttc gggtcgtcct cggtgtcgaa gcgctccgag gtcgggtcga cgtcgggcga
     6001 atagacataa cggtagaccg tgtccgtcgc gggctgcggc gccgccagcg cggtctggga
     6061 cgcgacctcg acctcctcct cgaccttcgc cttgatggcg tcgagctccg cctgcgtggc
     6121 gaggccctga tccaggaggt acttcgggaa ccggacgagc ggatccttcc gggcctccgc
     6181 ctcccgctcg gccgtcgggc ggtagaacac ctcgtcgtcc gagagcgagt ggctgtaggg
     6241 ccggatcacg tgggcgtgga ccagcgcggg gcccttccgc tggcgggcgt agtcgaccgc
     6301 gcgggtgagc gtctcgaatg acgccacggg gtcgcagccg tcacactcct cgacgtagag
     6361 gccggggaag ccggtgacga tgcgcgagat gctccccccc ggcgtgttca cctcgaccgg
     6421 caccgagatc gcgtagccgt tgtcctcgac cacgaacacc accggcagct tcaggttgct
     6481 ggcgctggag agcgcctccc agaactcgcc ctcgctggtg gtgccgtcgc cggtggagca
     6541 gaagaccacc tcgtccttcc ggaccatcgc gtcgcgatcg gggagcgccg ggttctgctc
     6601 ggcgcgcagc catgcctcgg cgcagccgac cgcctggagg aactgggtcc cggtggggct
     6661 cgacgagctg acgatgttga ggtccttcgc accccagtgg gagggcatct gtcgcccgcc
     6721 ggagttgggg tcgtcggccg cggcgacagc ggagaggagc tggtcggtgg ccgtctggcc
     6781 caggccgaga cagagggcgc ggtcgcggta gtaggtgtag aaccagtcgt aggcggggcg
     6841 gaagaccttg gccgcggcgg cgaggaccgc ctcgtggccg gcgccggaga tctggaagaa
     6901 gatcttgttc tgccgcttga gctggatctc cttgtcgtcg atgcgacggg agagcagcat
     6961 cacgcggtag atgtcgagga gatccgcctt gctgagggac ttggcggtct tcggctgggg
     7021 tcgggttcgg gtggccatgg cggaaaaata ggcctatgga cgcgggtggg ccagcagcgc
     7081 gccggggcgc cggtacacca ccttcccgcc gacgacggtc agcaccgccc gggcggtgcc
     7141 gagctccgcg ggcgggagcg tgaagaggtc tttgtccagc accgtgatat ccgcccactt
     7201 tcccggcgcc agcgtgccgc gatgcgcctc ggtgaagttg gcgtacgcgt tgccccaggt
     7261 gtacgcccgc agggcctcgc tgagcgtgat cttctgctcc ggcacccacc cgccgggatt
     7321 cttgccgtcg gaggtgcgcc gggtgacggc cgcggcgatc ccctggatcg ggtcgagcgt
     7381 ggcgacggtc cagtcggacc cgaacgccag ctcggcgtgg gtgtcgagga gggtgcggaa
     7441 cggataggtg gaatggatcc gcaccgggcc gatgcggtgc tggatccagc ggccgtcatc
     7501 gaccaggtgg tagggctgga ccgatgcgat cacgccgagc cggccgaaac gggcgatgtc
     7561 ctgcgggcgc aggtgctggg catgctcgat ccggaagcga cggtcacggg ggccgtgggc
     7621 ggtggtgagg ctgtcgtaga tcccgaggag gatggcattg gccgagtcgc cgatggcgtg
     7681 gacggcgacc tgcaggtgca ccgagtcggc ggcgccgatc cagcgggcca tgtcggcggg
     7741 ggagttgcgc agcaggccgc gatagccggc cgagtcggcg tacggctgcg cgaagaatgc
     7801 cgtgcgcgat ccggccgacc cgtccatgaa ctccttgagc ccgccgatgc ggacccagtc
     7861 gtcccccgcg ccatccgcgg cgatggtgtc ggccaccgcg tgccagttct cgagcggcag
     7921 gtagagcgcc gcccgcatcg tgagccgtcc cgcacgctcg acccgccgga agctctggag
     7981 gtcggcgaac gacgcccaca tcgacgaggt gccggtgacg ccgagcgagg cggcgtaggc
     8041 cagggcgcgg acgagggccg aatcccgctc ctcgggggtg ggatccggga cgaccgcata
     8101 gaccatccgg agcgcctgat ccttgaagat cccggtcagc tcgccggtgc ggggatcgtg
     8161 gccgatctcc cctcccggcg gggctgccgt ggcacgggtg atcccggcgg cccgcatcgc
     8221 ggcggcgttg gccagcgcct cgtggccgtc gagtcgctca aggaacaccg ggttgtcggg
     8281 ggtcaccgag tcgatccact cgtgccgcgg gagcggctgg cccggccaga gggtgtgatc
     8341 ccagtcgccg ccgatgatcc accgcccctt ggggacgtgg cggacgaagt cgcggatgcg
     8401 gcggatgaac tccgccggtg tggccacggt gcggaggtcg agcgaggcga gctggaaccc
     8461 gccgtcgatg aagtgggtgt ggccgtcctg gaatcccggc gtcacgagcc ccggcgcatc
     8521 gacgatctcg gtggtgggac cgatgaggcg cgcgaccgac gcgcggtcgc cgatggcggc
     8581 gatcgtgtcg cccgctacgg cgagcgcgga ggcccacgga tgggccgggt cgccggtcca
     8641 gatgcggccc cgaatgacga gggtggcctg ggcggcggcg ctcgaaccgt gcatcagcag
     8701 cgcggtgagc gccagcaaca tgaatgcgga ccgacggtgc attgacgctc cttcggggcg
     8761 gctagcggag cttggtgatc cggcggaggg cgtcgagctg ctgcagggca gtcccaggcc
     8821 cctgcgacgg cgcggcggga cgggcagcga gcaggtcacg cacgtccagg aagacctcca
     8881 tcatcagctg gtcccgctgg taggcggcat caagctcctc catccggcgg gtctgccctt
     8941 ccgccttggc cgcggcgtac gagtcacgat agatggtgtc gaggttgccg aggatgtggt
     9001 cgcgcgggcg catgggatcg gggcaccggt cggaagaggg aagggccgag gtgaagctaa
     9061 cgagcggcgg cccggtcgga agggtgttgt gcccgggagc cggaaaagcg ataatggtgc
     9121 catctcgacc cccgggacgc gccgatggcg aagctgatga agctgttcct caccctgctc
     9181 ctcctctccg ggctcgccgc cgcaggctcc tacggcgtgg cctacctggc ccagggcaag
     9241 ctggtgggcg cgcagccgct ggaattgggc gagcagcaga cccgctttgc cacggaaggg
     9301 atcgcgggcc tgccgggggc cccgaaggcc tgggtgttca cctatggccc gacgaagatc
     9361 ccggggatga agctggccga gatctacgtc tccccgctgg gcgccgtgat cggcaccaag
     9421 cccaaggact tctcggcccg gctgcaggcg taccgcgccg cgcagtcccc ggactgaccg
     9481 gccacgatgg cgttccgtga cgcgcagacc cgggtggacg cgtggatctc gcagttcgag
     9541 gagggatact tcgagcccct gaccaacctg gcccggctca ccgaagaggt cggggagctg
     9601 gcgcgggagc tcaaccaccg ctacggccag aagaccaaga agcgggacga gcccgagggt
     9661 gacctgcccc tcgagctcgc cgatatcctc ttcgtcctca tctgcctggc caaccgcgaa
     9721 ggggtcgacc tcgaccaggc attcgaccgg atgatgcaga agatcgaggg ccgcgaccgc
     9781 gaccgctgga cccgcaagcc ccccgcctga cccgcgttca ctcccagtcg gccggttccg
     9841 cctccgtctc ctccagcagc agtcgatagc tcgccagccg gtcgggatgc accttgccct
     9901 ccgcggcggc cgcggcgatc cggcaccccg gctcgctcac gtggcggcaa tccggaaagc
     9961 ggcactgacc gaggagcggc cggaactccg ggaagcacgc ggccagttcc ctcggctcca
    10021 ccccccacaa tccgacctcg ctgaagcccg gcgtatcgac caggaaggcg tcggcgccga
    10081 agggcaccat caccgccccg acggtggtct ggcgcccgcg cttccccctg tcgctgatgg
    10141 cgccggtgcg gaggacgagg ccgatcccga tggcattgag caggctcgat ttccccgcgc
    10201 cggaagggcc ggtgaccacc gactccccgt ggacgaggag cgcgcggagc tgggcgatgc
    10261 ccgccccggt cttcgcgctg gtcgggacca ccgtgtagcc agcatcggcg taccgccgca
    10321 ccagcgcctc gccgggatcg agatccgtct tgttgaccac cagcaccgcc gggaggtcgt
    10381 tgacctcggc caccaccagc aaccgatcga ggagcgaggg gatgggggcg gggtcgatgc
    10441 tggccgtgac cacgatgacg cgatccacgt tggcgaccac cggccgcgcg ccccggccga
    10501 gcggcacccg tcgctcgacg atgctgcgcc gcggctccac cgcggcgatc ccgtagagct
    10561 ccccctgcgg cgcgtcctcc aactggatcc ggtcgccgac gatcacctgg ggcgtttgcc
    10621 gcttgagctt gccgcggagc actgcccgga cctcggcgcc
//
LOCUS       DASUAP010000327         6899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1359891, whole genome shotgun sequence.
ACCESSION   DASUAP010000327 DASUAP010000000
VERSION     DASUAP010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359891"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFU45_08930"
     CDS             complement(<1..590)
                     /locus_tag="VFU45_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359231.1"
                     /translation="MEERRVVIRLAPLALALGIALPCGAQVPVAQDTAMSAAAMESMA
                     HPMAEPHMRMTAPRPASAADSARGTALVALIRRELARYRSVDTALADGFRPFHPEVPQ
                     PVYHYTNLMYGLEARFTFDPEHPTSLLYRKDADGGFTLVGVMYTAPRYLADSALDARV
                     PLSLTHWHEHVNWCVPPRGAGERWRERRDGAPVFGP"
     gene            complement(569..2905)
                     /locus_tag="VFU45_08935"
     CDS             complement(569..2905)
                     /locus_tag="VFU45_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU5359232.1"
                     /translation="MRQLALLPLMLAAAPALAAQAAAPVRDVPVVAPVATSVDTVIRQ
                     LKALAPVPGTSGEVHGLALRRGTGVFDLTSGKLYPLTPIGGRTIALLFVGSGRFLASP
                     PDPVERGMLATALGHGDSTLDAAFHSAVFVFADSTWEEVSAHAAFAADPVRPDTKPVG
                     NALDVLGGVDRSHYSASLLGALLDGERSGYFYGFVEPDQGPALGLEVDPDLVESESIL
                     RKASHTGVYHWMDVLATFPAAGTGASAPKETTRTDAVTDYTMDLDLPESGGSLAFNAR
                     ADLTVVPDGPMGPWIPLSLYYKLLGDSASTGGHPTAVWKVKDDPTMWVKLDHRLSAGD
                     TARVRVQYHGDLIDRFGDWFLVNPTSDWYPVPTDYRNYATFDLTFHTPQQYPILSVGD
                     RSDSTTEPGHMLRTRWVIHTPIRNAAFNIGVFSESHLTDAGVPPVTFLYSDHAIKYTL
                     ANGGSFVVTPDKKERENVGHDIQNALKFFTSVYGGPPVDRFYAGPIPYSEGLAFPGMI
                     DLSMSTFEGGNGSQKGFDQFFRAHEVSHQWWGIGVDYASYRDQWLSEGLATFSGLWFM
                     QVAFHDNKLYFDYLDRYRDDIAAVADAGPVAIGYRTESDAHPSGYQTLVYEKGAWMAH
                     MLRILMLDLRTMSEGQFTQAMQSFYSSYHGGRASTADFQHLFERHLGIPLDWFFKEYY
                     DGTALPAYQTAWKADAQPDGSYTVHLRVTQSGVPADFENYIPVAMDLGGGRVAHLRMH
                     VVGASSTLDVPGMPAQPKAVKFNDLDGVLAASWKSVGW"
     gene            complement(2905..3660)
                     /locus_tag="VFU45_08940"
     CDS             complement(2905..3660)
                     /locus_tag="VFU45_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEU5359233.1"
                     /translation="MSEPEHEWSRRGFVAAAGSAAAFAAVGRRLDPQAAVTSDTPGPG
                     RVILFQGDSITDCGRDRDVTAANSAAALGNGYPLLIAAARLAAHPGRGYRFFDRGVSG
                     NTVPDLDARWQADTLDLEPDVLSILIGVNDYWHTLGGDYHGTVDDYARGYAALLARTR
                     RALPKVRFVVLEPFVLRTGVVKDSWFPEFDQRREAARRVAREADATFLPLQKMFDELS
                     REAPPVYWSLDGVHPTAAGHAAIADLWTRKVKL"
     gene            complement(3896..4783)
                     /locus_tag="VFU45_08945"
     CDS             complement(3896..4783)
                     /locus_tag="VFU45_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359234.1"
                     /translation="MHHRIRLYALAGIAALGAAACSSGSSSPSAPAVSALLADSLGEA
                     VVGDINVSTAGATTTGASASFQASAGAELAGPDLSSPSCTVTRSPSSPANSDADPVPD
                     SVRITFTSCVFSSTLETDTLKGTIDFIDPTPTTTDRAIKRVFTDYGWVTVKTLSGAVR
                     SVLMSGTRMAVRDSSTITQTDSNMTTTFTFANGNTATHDRTWSFHFTADTANTIKPDS
                     ALPAGDWTVTGNSTWAHGLLTFDLALTTPTPLHFNPACTEAPRFDAGVTRVVVTRGGN
                     TSTLLIQHTACGQFTVTRS"
     gene            complement(4931..5422)
                     /locus_tag="VFU45_08950"
     CDS             complement(4931..5422)
                     /locus_tag="VFU45_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359235.1"
                     /translation="MTGHPPLRPAAQRSGLIGAALLLGGLGIYAFSRWWGTPSLGDLR
                     VDHVTFASSRSIIDRHFNRGLILVTSGGTYALWVRPTLRDSVAARLHPGDSLVTWSTA
                     AGGGYRTPWQVVRGADTIVAYTDRAVVDRERDRRGRGFGRWMMLAGLALLFVALALGR
                     RVD"
     gene            complement(5419..6033)
                     /locus_tag="VFU45_08955"
     CDS             complement(5419..6033)
                     /locus_tag="VFU45_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEU5359236.1"
                     /translation="MRHLVQAALFLFATIDPLGTVLLFSAVTAGIAPAERHAIARRAV
                     VSAGAILVGAMVLGQPILQGLGVRLISLQVAGGLILFLFGLQMIFGEVGAPGRNQPES
                     GHDVAVFPLAVPAIASPGAVIAVIVLTDNDVYSLASQATIGAILLAILLLTWAMLRLA
                     GPILSVLGVSGSRVLIRVLGMLLAALAVELTLSALGVAGWTGPA"
     gene            complement(6070..6534)
                     /locus_tag="VFU45_08960"
     CDS             complement(6070..6534)
                     /locus_tag="VFU45_08960"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU5359237.1"
                     /translation="MSPTVYAMIPAAGARAAWILVPIILLVAGAVATLGYTIYGSHNA
                     TFELSDEGLAFHGDIWGRRIPLSELRGGAARIVDLTAEPGLKPVSRTMGTALPSYQSG
                     WFRLADGEKALLYLTSRTRALYIPTTRKFSVLLSPQDPDRMLADLRRLAPGS"
     gene            complement(6531..>6899)
                     /locus_tag="VFU45_08965"
     CDS             complement(6531..>6899)
                     /locus_tag="VFU45_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU5359238.1"
                     /translation="VIQYAVDILAVKHIIVTGHHGCGGIIAALGPRAPEPLETWLTPI
                     RAVRDRHADELAGLDPAERPDRLCELNVAAQVRSVADTPTVRGAWAAGKPLAIHGWFY
                     DLKDGLLHDLDVTLEGPEAK"
BASE COUNT         1049 a         2259 c         2602 g          989 t
ORIGIN      
        1 tggggcccga agaccggggc gccgtcgcgc cgctcgcgcc agcgttcgcc ggcgccgcgg
       61 ggcgggacgc accagttgac gtgctcgtgc cagtgggtga gcgagagcgg gaccctggca
      121 tccagggccg agtcggcgag gtacctcggg gcggtgtaca tcacgccgac gagcgtgaag
      181 ccgccatcgg cgtccttgcg gtaaaggagc gaggtcgggt gctccgggtc gaaggtgaag
      241 cgggcctcga ggccgtacat caggttggtg taatggtaga ccggctgcgg cacctcgggg
      301 tggaagggcc ggaagccgtc ggcgagcgcc gtgtcgacgc tccggtaccg ggccagctcg
      361 cggcggatca gggccacgag cgccgtgcca cgggcggaat ccgcggccga ggcgggccgg
      421 ggcgccgtca tccgcatatg cggctctgcc atcgggtggg ccatgctctc catcgccgcg
      481 gcactcatcg ccgtgtcctg cgccaccggc acctgggcgc cgcacggaag cgcgatcccc
      541 agcgcgagcg ccagcggcgc cagccggatc accacccgac gctcttccat gaggcggcca
      601 gcacgccgtc gaggtcgttg aacttgacgg ccttcggctg ggccggcatc cccgggacgt
      661 cgagcgtgct cgaggccccg acgacgtgca tcctcaggtg cgccacccgg ccgccgccga
      721 ggtccattgc gacggggatg tagttctcga agtccgccgg cactccggac tgcgtcaccc
      781 gcaggtggac ggtgtagctg ccgtccggct gggcgtcggc tttccacgcg gtctggtagg
      841 cggggagcgc cgtgccgtcg tagtactcct tgaagaacca gtccagcggg atcccgaggt
      901 ggcgctcgaa gaggtgctgg aagtccgccg tcgaggcccg gccgccgtgg tacgacgagt
      961 agaagctctg catcgcctgg gtgaactgcc cctcgctcat cgtccggagg tcgagcatca
     1021 ggatgcggag catgtgggcc atccacgcgc ccttctcgta caccagcgtc tggtatccgc
     1081 tcggatgcgc atcgctctcg gtccggtacc cgatcgcgac ggggccggcg tcggccaccg
     1141 cggcgatgtc gtcccggtac cggtcgaggt agtcgaagta gagcttgttg tcgtggaacg
     1201 ccacctgcat gaaccagagc ccggagaagg tggccagccc ttcgctcagc cactggtccc
     1261 ggtagctggc atagtcgacg ccgatgcccc accactggtg gctcacctcg tgcgcccgga
     1321 agaactggtc gaaacctttc tggctcccgt tgcccccctc gaaggtgctc atcgacaggt
     1381 cgatcatccc ggggaacgcg aggccctcgg agtacgggat cggcccggcg tagaagcgat
     1441 ccacgggcgg cccgccataa acgctggtga agaacttgag cgcgttctgg atgtcgtggc
     1501 cgacgttctc gcgttccttc ttgtccgggg tgacgacgaa gctcccgccg ttcgcgagcg
     1561 tgtacttgat cgcgtggtcg gagtagagga acgtgaccgg gggcacgccg gcatcggtca
     1621 ggtgcgactc ggagaacacc ccgatgttga acgccgcgtt ccggatcggg gtgtggatga
     1681 cccagcgggt ccgcagcatg tggccgggtt ccgtggtcga gtcgctccgg tcgccgacgc
     1741 tcaggatcgg gtactgctgc ggcgtgtgga acgtcaggtc gaacgtggcg tagttccggt
     1801 agtccgtggg caccgggtac cagtcggacg tcgggttgac caggaaccag tcgccgaacc
     1861 ggtcgatgag gtcgccatgg tactggaccc gcacccgcgc ggtgtcgccg gccgagagcc
     1921 ggtgatcgag cttgacccac atcgtcgggt cgtccttgac cttccacacc gccgtcggat
     1981 gtccgccggt gctggcggag tcgccgagga gcttgtagta gagcgacagc gggatccagg
     2041 gacccatcgg cccatccggc acgacggtca ggtccgctcg ggcgttgaaa gcgagcgagc
     2101 ccccggattc cggcaggtcg agatccatcg tgtagtcggt gacggcgtcg gtgcgggtcg
     2161 tctccttcgg ggcgctcgcg cctgttcccg cggctgggaa ggtggcgagc acgtccatcc
     2221 agtggtacac gccggtgtgt gaggccttgc gcaggatgga ttccgactcg accaggtccg
     2281 ggtcgacctc cagcccgagg gctgggccct ggtcgggctc cacgaagccg tagaagtaac
     2341 cgctccgctc gccgtcgagc agtgcgccga gcaggcttgc ggagtagtgc gatcggtcga
     2401 cgccgccgag cacgtccagg gcgttgccga cgggcttggt gtcggggcgg accggatcgg
     2461 cggcgaaggc ggcgtgggcg gagacctcct cccacgtgga atcggcaaag acgaatacgg
     2521 ccgaatggaa cgcggcatcg agcgtgctat cgccatggcc cagcgcggtg gcgagcattc
     2581 cgcgctcgac cggatccggc ggggaggcca ggaaccggcc gctcccgacg aagagcagcg
     2641 cgatggtgcg gccaccgatc ggcgtgagcg ggtagagctt cccggacgtc aggtcgaaga
     2701 caccggtgcc acggcgcaag gcgaggccgt gtacctcccc ggacgtgccg ggaacggggg
     2761 ccagggcctt gagctggcgg atgaccgtgt ccaccgacgt ggccaccggt gccaccactg
     2821 ggacgtcgcg gaccggggcg gccgcctgcg ccgcgagggc gggcgcggcg gcgagcatca
     2881 acgggaggag cgccagctgt cgcatcagag cttcaccttt cgcgtccaga ggtcggcgat
     2941 ggcggcgtga ccggcggcgg tcgggtggac cccgtcgagc gaccagtaca ccggtggcgc
     3001 ctcccggctc agttcgtcga acatcttctg cagcgggagg aaggtggcgt cggcctcccg
     3061 ggccacccgc cgggcggcct cgcgccgctg gtcgaactcg gggaaccagc tgtccttcac
     3121 cacccccgtg cggagcacga agggctcgag caccacgaac cgcaccttgg ggagcgctcg
     3181 ccgggtgcgg gcaaggagtg cggcgtagcc gcgggcgtag tcgtccacgg tgccgtgata
     3241 gtcgcccccc agggtgtgcc agtagtcgtt gacgccgatg aggatgctca gcacgtccgg
     3301 ctcgaggtcg agcgtgtcgg cctgccagcg cgcatcgagg tcggggacgg tgttcccgct
     3361 cacgccgcgg tcgaagaagc gatagccgcg ccccgggtgc gccgcgagcc gggcggcggc
     3421 gatgagcagc gggtagccgt tgccgagcgc cgcggcgctg ttcgccgccg tgacgtcacg
     3481 gtcccgcccg cagtcggtga tcgagtcacc ctggaagagg atcacccgtc cagggcccgg
     3541 cgtgtcggag gtgaccgctg cctgcggatc caaccggcgc cccaccgccg cgaacgcggc
     3601 ggcgctgccg gcggccgcca cgaacccgcg gcgcgaccac tcgtgttccg gctcgctcat
     3661 gacgatgttc ggccgcgctc ggcaccttgc cgccgcgacc gcccttcgtg ctgtgatggt
     3721 gtgtgagaca gatgccggcc aaggcagcac aaatagtgct aaggagccgc cctgaacgcc
     3781 actagggaaa accctagtgg caaggaattg gacagtaggc cggtcacgat gccgtactaa
     3841 tacggaggcc cgggccgccg acgtggcgga ccgggcctcg cgatcggcgg gaccgtcagc
     3901 tgcgggtgac ggtgaactgc ccgcaggcgg tgtgctggat caggagcgtc gaggtgttgc
     3961 cgccccgggt caccaccacc ctggtgacgc cggcgtcgaa gcggggcgct tccgtgcacg
     4021 ccgggttgaa atgcagcggc gtcggcgtgg tgagtgccag gtcgaaggtc aggagcccgt
     4081 gggcccaggt ggagttgccg gtcacggtcc agtccccggc cgggagcgcc gagtcgggct
     4141 tgatcgtgtt cgcggtatcg gcggtgaagt ggaacgacca ggtccggtcg tgggtggcgg
     4201 tgttgccgtt cgcgaaggtg aacgtggtgg tcatgttgct gtcggtctgc gtgatggtgc
     4261 tcgagtcacg gacggccatc cgcgtgccgc tcatcagcac cgaccgcacg gcgccgctca
     4321 gcgtcttcac ggtgacccag ccgtagtcgg tgaacacccg cttgatggcc ctgtcggtgg
     4381 tggtgggcgt cgggtcgatg aagtcgatcg tgcccttgag cgtgtcggtc tccagcgtgg
     4441 aactgaatac gcagctggtg aacgtgatcc gcaccgagtc cggcaccggg tcggcgtcgg
     4501 agttggcggg cgaggacggg ctccgggtca cggtgcagga gggcgagctc aggtccggac
     4561 cggccagttc cgccccggcg ctcgcctgga aggaggcgct ggcgcccgtg gtggtggcgc
     4621 ccgcggtgct cacgttgatg tcgccgacga cggcctcgcc gaggctgtcg gcgaggagcg
     4681 cgctcacggc gggcgcactg gggctcgagc tcccggaact gcacgcagcg gcgccgagtg
     4741 cggcgatccc ggcgagggca tagagacgga tacggtggtg catcgcttcc tccggccagt
     4801 aaggagaaga cctgacagtg ccgggcggac gccgccgggt cggcggtgtt aagccggcct
     4861 caccgtggcg cgctatataa ggatggggac cgggtgtgac ctgcgtcaca ccggcctgtg
     4921 gccaccacga tcaatccacg cggcgaccca gcgccagtgc gacgaacagc agcgccagcc
     4981 cggcgagcat catccaccgg ccgaagccgc gcccgcgccg gtcgcgctcc cggtcgacga
     5041 cggcgcggtc ggtgtaggcg acgatggtgt cggcaccccg cacgacctgc cacggggtcc
     5101 gatacccgcc tccggcggcg gtactccagg tcacgaggga gtcgcccgga tgaagccgcg
     5161 ccgcgacgga gtcccggagc gtgggacgca cccagagcgc gtacgtgccg ccgctcgtga
     5221 ccaggatgag tccgcggttg aagtgccggt cgatgatcga ccggctggac gcgaaggtca
     5281 cgtggtcgac ccgcaggtcg ccgagggacg gcgtgcccca ccagcggctg aaggcgtaga
     5341 tgccgaggcc gccgagcagc agcgcggcgc cgatcaggcc cgagcgctgc gccgccgggc
     5401 ggagcggtgg atggccggtc acgccgggcc ggtccagccc gcgaccccga gggcggagag
     5461 ggtgagctcc accgccagcg cggcgaggag catgcccagg acccggatga ggacccggga
     5521 gccgctcacg cccagcacgg agaggatggg accggcgagc cggagcatgg cccaggtgag
     5581 cagcaggatg gccagcagga tggcgccgat cgttgcctgg ctggcgaggg aatacacgtc
     5641 gttgtcggtc agcacgatca ccgcgatgac ggcgcccggg cttgcgatcg ccggcacggc
     5701 aagggggaac accgccacgt cgtggccgga ctcgggctgg ttccgtcccg gcgcgccgac
     5761 ctcgccgaag atcatctgca ggccgaacag gaagaggatg aggccgcccg ccacctgcag
     5821 cgagatgaga cggacgccga gcccctggag gatcggctgg cccagcacca tcgcgccgac
     5881 gaggatcgcc ccggccgaga ccaccgcccg tcgtgcgatg gcgtggcgct cggccggggc
     5941 gatgccggcc gtgacggcgc tgaagagcag taccgtgcca agtggatcga tggtggcaaa
     6001 aaggaagagc gcggcctgga ccaggtgacg catcgggatc ctccgctcga cgtcccccgg
     6061 gagggccggc tagctgccgg gcgccaggcg tcggaggtcc gcgagcatcc ggtcggggtc
     6121 ctgcgggctc aacagcaccg agaacttccg ggtggtcgga atgtagagcg cgcgcgtgcg
     6181 ggaggtcagg tagagcaggg ccttttcgcc gtcggccagg cggaaccacc ccgactggta
     6241 ggacggcagc gccgtgccca tcgtgcgcga caccggcttg aggcccggct cggcggtgag
     6301 gtcgacgatc cgcgcggcgc cgccgcggag ctcggacagc gggatccggc ggccccagat
     6361 gtcgccatgg aaggcgagtc cctcgtcgga gagctcgaag gtggcgttgt gcgagccata
     6421 gatcgtgtag ccgagcgtgg cgacggcgcc cgcgacgagg aggatgatcg gcaccaggat
     6481 ccacgcggcc cgtgcgccgg ccgcagggat catcgcgtag acggtgggac tcacttcgcc
     6541 tccggccctt cgagggtcac gtcgaggtcg tggagcagcc cgtccttgag gtcatagaac
     6601 cagccatgga tggccagcgg cttcccggcg gcccaggcgc cccgcaccgt gggcgtgtcg
     6661 gccaccgacc ggacctgggc ggccacgttg agctcgcaga gccggtcggg ccgctcggcg
     6721 ggatcgagcc cagccagctc gtcggcgtgg cggtcgcgca cggcgcggat gggggtgagc
     6781 caggtctcca gcggctccgg cgcccgcggg cccagcgcag cgatgatccc gccgcagccg
     6841 tggtggccag tgacgatgat gtgcttcacg gccaggatgt ccaccgcata ctggatgac
//
LOCUS       DASUAP010000328         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_692109, whole genome shotgun sequence.
ACCESSION   DASUAP010000328 DASUAP010000000
VERSION     DASUAP010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692109"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFU45_08970"
     CDS             complement(<1..213)
                     /locus_tag="VFU45_08970"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEU5359239.1"
                     /translation="MSKRAVVLLSGGMDSATTLAIARSEGFACHALSFRYGQRHAIEL
                     ERAATVARMLGAVEHRVVTLDPAAFQG"
     gene            complement(241..1620)
                     /locus_tag="VFU45_08975"
     CDS             complement(241..1620)
                     /locus_tag="VFU45_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEU5359240.1"
                     /translation="MTRRYTRSSPMPVPVEELFDWHLRPGAFERLAPPWERADVMART
                     GPVADGAVVTLHVPIGPVSVRWVSRHRDVHRPSGFVDEQLSGPFASWVHAHRMEPSAA
                     GGSTLTDDIAYALPFGPVGAIAAGGVRRKLERVFAYRHRITRGDLAAHASFRDRPRLH
                     VAITGASGFIGRALEAFLTTGGHRVTRLVRRAPAAGEVRWAPDAAADLAPLGPVDAVI
                     HLAGEGIATARWSTRRKSAIATSRQRGTANLVASLLRLAAPPPVLVTASAIGIYGDRD
                     DDELTEASPVGTGFLADVGRAWEAASAPAEAAGIRVVRARLGIVLSPDGGALRKMLPP
                     FRLGIGGRLGSGRQWMSWVALDDAIGAIHHAIFTPSLRGPVNVTAPAPATNAEFTATL
                     ARVLARPAILPVPAAALTLVFGQLADEALLAGQRVLPAALLAAGYRFRDPVLEGALRH
                     VLGRTPLPD"
     gene            complement(1622..2722)
                     /locus_tag="VFU45_08980"
     CDS             complement(1622..2722)
                     /locus_tag="VFU45_08980"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5359241.1"
                     /translation="MRWSFRIGRLWGIELKVHVTFFLLLAWAAAEGFGAGGGRAAASS
                     VVVILAVFACVVLHELGHARMAAHFGVATSDITLLPIGGVARLERIPEEPRQEFLVAL
                     AGPAVTLALAALAWVVITLARLPAPAADAPLTAATLLQLLLEVNLYLLLFNLIPAFPM
                     DGGRVLRALLARRVGFVRATQVAAKVGRTIAVAFAVIGLFGIPGVYPVNPILVLIAAF
                     VWFGATAEATSVEYRAMGAALTAGQVMVRRFDTLPAWAPLERAADLLTSTDQRDFPVQ
                     DAGGRLVGLLTRDLLVLGLHRLGAAAEVRQVMATEVPVLTESAPFPAALEALLKSRLP
                     ALPVIDERGAVVGLFSKDNVADLLLVRRSAAP"
     gene            complement(2802..2887)
                     /locus_tag="VFU45_08985"
     tRNA            complement(2802..2887)
                     /locus_tag="VFU45_08985"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2853..2855),aa:Ile,seq:tat)
BASE COUNT          492 a         1020 c         1174 g          347 t
ORIGIN      
        1 accctggaaa gcggccgggt cgagcgtcac gacccggtgc tcaacggcgc cgagcatccg
       61 ggcgaccgtc gcggcgcgct ccagttcgat cgcatggcgt tggccgtacc ggaagctgag
      121 cgcgtggcac gcgaacccct ccgatcgggc gatcgcgagg gtcgtggccg aatccatccc
      181 accgctcagc agcaccacgg cacgcttcga catgcggtga atataggccc cgccgcggcg
      241 tcagtccggg agcggtgtcc gtcccaggac gtgacggagg gcgccctcca ggaccgggtc
      301 ccggaagcgg tagcccgcgg cgaggagcgc cgcggggagc acccgctggc ccgccaggag
      361 ggcttcgtcg gccagctggc cgaagacgag ggtgagcgcg gcggccggca cgggaaggat
      421 cgcggggcgc gcgagcaccc gtgcgagcgt agcagtgaac tcggcgttgg tggccggggc
      481 cggggccgtg acgttcaccg ggccgcgcag cgagggcgtg aagatggcgt ggtggatcgc
      541 gccgatggcg tcgtcgagcg cgacccagct catccactgg cgcccgctcc cgagccgccc
      601 gccgatcccc aggcggaacg gcgggagcat cttccggagc gcaccaccat cgggcgacag
      661 cacgataccg aggcgggccc gcacgacccg aatgccggcg gcctcggccg gggcgctcgc
      721 ggcctcccat gcccggccga catcggcgag gaatccggtc ccgaccgggg acgcttcggt
      781 cagttcatcg tcgtcacggt caccgtagat gccgatcgcc gatgcggtga cgagcaccgg
      841 gggcggtgcc gcgagccgga ggagggaggc cacgaggttg gccgtgccac gctggcggct
      901 cgtcgcgatc gccgacttgc gccgtgtgga ccaccgggcg gtggcaatcc cttcgcccgc
      961 gaggtggatg acagcgtcga ccgggccgag gggggcaagg tcggcggccg cgtcgggggc
     1021 ccagcgcacc tcgcccgcgg cgggtgcccg gcggacgagg cgagtgacgc ggtggccacc
     1081 ggtcgtgagg aaggcctcga gcgcgcgccc gatgaatccg gatgcgcccg tgatcgccac
     1141 gtggaggcgg ggccggtcgc ggaaggaggc gtgggccgcg aggtcgcccc gggtgatgcg
     1201 gtgccgatag gcgaagaccc gctcgagctt ccggcgcacg ccgccggcgg cgatcgcgcc
     1261 caccgggccg aaggggagcg cgtacgcgat gtcgtccgtg agcgtcgatc cgccggccgc
     1321 tgagggctcc atccggtggg cgtggaccca ggatgcaaag ggcccggaca gctgctcgtc
     1381 gacgaatccg gacggccggt ggacatcgcg atgccgcgag acccaacgga ccgataccgg
     1441 cccgatcggg acgtggagcg tcacgaccgc gccatcggcg acggggccgg tccgcgccat
     1501 cacgtcggcg cgctcccacg gcggagccag ccgctcgaag gcgccgggac ggaggtgcca
     1561 gtcgaagagc tcctcgaccg ggaccggcat cggcgacgag cgggtgtacc ggcgggtcac
     1621 gtcagggggc ggcggagcgg cgcacgagga ggaggtcggc cacgttgtcc ttgctgaaca
     1681 ggccgaccac cgcaccgcgc tcgtcgatga ccggcagcgc ggggagccgg cttttcagga
     1741 gcgcctcgag cgcggcaggg aagggggcgg actcggtgag gaccggcact tccgttgcca
     1801 tcacctgccg cacctccgcc gccgcaccga ggcgatggag gcccagcacc aggaggtcgc
     1861 gggtgaggag gcccacgagc cggccgccgg catcctgcac cgggaagtcg cgctggtcgg
     1921 tggaggtcag gaggtccgcc gcgcgctcga gcggcgccca ggccggcagg gtgtcgaagc
     1981 ggcggaccat cacctggccc gccgtgaggg cggcgcccat ggcgcggtac tcgaccgagg
     2041 tggcctcggc cgtggcgccg aaccagacga aggcggcgat gagcaccagg atcgggttga
     2101 ccggatagac gccggggatg ccgaagagcc cgatcacggc gaacgccacc gcgatggtgc
     2161 gccccacctt ggccgcgacc tgggtggcgc gcacgaaacc gacccggcgc gcgaggaggg
     2221 cacggagcac ccggccgccg tccatcggga acgcggggat caggttgaag agcaggaggt
     2281 agaggttgac ctcgaggagg agctggagca gcgttgcggc cgtgaggggc gcgtcggcgg
     2341 ccggggcggg aagtcgcgcc agcgtgatca cgacccaggc gagtgccgcc agggcgaggg
     2401 tgaccgcggg gccggcgagc gcgacgagga actcctgccg cggctcctcg gggatgcgct
     2461 ccaggcgcgc gacgccgccg atcgggagaa gggtgatgtc ggacgtggcc accccgaagt
     2521 gcgcggccat ccgcgcgtgg cccagttcgt ggagcaccac acaggcgaag accgcaagga
     2581 tcaccacgac cgatgaggcg gcggcgcggc cgccccccgc cccgaatcct tcggccgccg
     2641 cccacgcaag caggaggaag aaggtgacgt ggaccttgag ctcgatgccc cagagccgtc
     2701 cgatccggaa ggaccatcgc atgggcggag gatacccggc cgggggggcc gcgggccagt
     2761 cccgcccggc ggcgggtagt gggtcaggct gactgtaaca aaggagagta cgggattcga
     2821 acccgtggac ccccgaaggg gccggtgagg ttataagcct acctgctttg ccaacgacga
     2881 actctcccac tcatataagt agtcggcaac ctcaccaaaa cgccgttttc cgggggatgg
     2941 actcacggat tggcccactt gacaatgctt gagcgctcaa gtatactgtt acccatgaac
     3001 agtctgcccg ataccacccg cgccgccatt gtc
//
LOCUS       DASUAP010000329         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_106474, whole genome shotgun sequence.
ACCESSION   DASUAP010000329 DASUAP010000000
VERSION     DASUAP010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106474"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1775
                     /locus_tag="VFU45_08990"
     CDS             <1..1775
                     /locus_tag="VFU45_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU5359242.1"
                     /translation="EVTQIEEAEEESDLLATTEEASARPVVRLVDAILSEGILARASD
                     IHIEPSEGGVVVRYRIDGVLVQKMKIPRSAGVPLISRIKIISSLDIADRLRPQDGRAR
                     MAVNGQPVDLRVSTLPASLGEKVVIRILNAQATALDLDALGLYPEETTALKALLDNKD
                     GIILVTGPTGSGKTTTLYSAIRFVQDEGVNIVTVEDPVEYRLSNNIVQVQVNPKAGLT
                     FASALRSILRQDPDVVLVGEIRDIETAQIAVQASLTGHLVLSTLHTNDAPNAVTRLMD
                     MGLEGFKIASALRGVVAQRLMRRLCPTCREVSTDPVPEKLRKHIPTGAKLNRAVGCPE
                     CAMTGYRGRFSIVEVLAMTPELERRIGAGAPPDQLAEAAKLGGMKNLWDSGLRHVLDG
                     HSSIEELLRVVDVPTSLPPVSRPSAATGSPAAAVEPSAAAAASPAVTAPTADVALSFD
                     LIEREGAAAEPSRRAAGAKILLVDDEDQLRRLLKDLLEREGYEVVEARDGAEALEQVD
                     RHGPDVMLLDLNLPGMDGYSVLAHVRSRPATEALPIVVLTAKGDEDNEVRVLQAGADD
                     FLTKPFRARALYARLEKIMARRKA"
     gene            1789..2256
                     /locus_tag="VFU45_08995"
     CDS             1789..2256
                     /locus_tag="VFU45_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5359243.1"
                     /translation="MSDIRVTYVDVYVLRGTGDAVEALVLRRSSAGRCPGAWESVHGG
                     IEPGETPVTAALRELREETGLVPGRLYNLSRVEAFYRHQQDFLALIPVFVALVPGGAE
                     VRLSGEHDAFDWLPPPAAGARMAWPRERRALEDAVALLSGGDAGAVEDVLRVC"
     gene            2250..3686
                     /locus_tag="VFU45_09000"
     CDS             2250..3686
                     /locus_tag="VFU45_09000"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEU5359244.1"
                     /translation="MLTRALVPGLLASALLTGAGSSAAPCLTIPRAGMRVAGDVRICP
                     GAYRVPDPDEKGVLVVTASGTRIDLTGVTIRSGDSIPSRYVGIGVVSRGNDEVEVVGG
                     VIGGYRYGIRLEGGRGHRISGVDVSGSRAQPLQSTPIRFADSDWLDIFDPDTFETYGG
                     GVYLKWTDGATVTGVQAHGGQNGIGLFGSSNGYIADNDVSGNSGWGIHLWQSSHNVII
                     RNVAHTNVRCEAAAYSRGCDSAALLLRNACDSNLIADNDLRRSGDGFFLSGQPPTMAP
                     SDGNVVSGNDGSSSPHNAFESTFSDGNTFLENRADSSDYGYWLGYSSRTLVRGSTVVG
                     SRSAGIAIEHGRDNELRQNLIVGGNVGIRLFAPGGGPVPSRGTTVADNTIARVGRGLV
                     LEGTTGSSALGNVFDGVGTALVVDSAGWNTTVRGNVFLGARDAWIVAPDLAAGDNYWA
                     AADVAATVARVRGRISILPWHPASAAGY"
     gene            complement(3680..>4349)
                     /locus_tag="VFU45_09005"
     CDS             complement(3680..>4349)
                     /locus_tag="VFU45_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5359245.1"
                     /translation="SVATTGAEHGRVRLETSAQDGRVVLRVTDTGAGVAPEHVPLLFT
                     PFFTTKEPGQGTGLGLSISYGIVEAHGGRLEYAPAPGGGATFSITLPHLEAQALAGRP
                     PRRILLADDDPKNERLVAALFARDGHEVEAATSADDALARLAAGRYDLIVVDARMPAP
                     GSGTLVAVLLDAHPELGRRLVVAVPRSAPGMAERFRAAGHLVVQHPYDLRALRSAAEE
                     VFGQ"
BASE COUNT          610 a         1566 c         1578 g          595 t
ORIGIN      
        1 acgaggtcac ccagatcgag gaggcggagg aggagtccga cctcctggcc accaccgagg
       61 aggcgtcggc ccgccccgtg gtgcggctgg tggacgcgat cctctccgag ggcatcctgg
      121 cgcgggccag cgacatccac atcgagccct ccgagggggg cgtggtggtc cgctaccgca
      181 tcgacggcgt gctggtccag aagatgaaga tcccgcgctc cgccggcgtc ccgctcatct
      241 cccgcatcaa gatcatctcg tccctcgaca tcgccgaccg gctgcgcccg caggacggcc
      301 gcgcgcggat ggcggtgaac ggccagccgg tggaccttcg cgtgtccacg ctcccggcct
      361 cgctcggcga gaaggtggtc atccggatcc tcaacgccca ggcgaccgcg ctcgacctcg
      421 acgcgctggg gctctatccg gaggagacca ccgcgctcaa ggcgctgctg gacaacaagg
      481 acggcatcat cctggtgacg gggccgaccg gctccggcaa gaccaccacg ctctattccg
      541 cgatccggtt cgtgcaggac gaaggcgtca acatcgtcac ggtcgaggac ccggtcgagt
      601 accggctctc gaacaacatc gtgcaggtgc aggtcaaccc gaaggcgggg ctgacgttcg
      661 cgagtgccct ccggtcgatc ctgcggcagg acccggacgt cgtcctggtc ggcgagatcc
      721 gcgacatcga gaccgcgcag atcgcggtgc aggcctcgct gaccgggcac ctggtgctct
      781 cgaccctgca caccaacgat gcccccaacg ccgtcacccg cctgatggac atggggctcg
      841 aggggttcaa gatcgcgtcg gcgctccgcg gtgtcgtggc ccagcggctg atgcggcgcc
      901 tctgccccac ctgccgcgaa gtgtcgaccg acccggtgcc ggaaaagctc cgtaagcaca
      961 ttccgacggg cgccaagctc aatcgcgcgg tcggctgccc cgagtgcgcg atgaccggct
     1021 accgcgggcg gttcagcatc gtcgaggtgc tggcgatgac gcccgagctc gagcgtcgga
     1081 tcggcgccgg ggcgcccccc gaccagctgg ccgaggcggc caagctcggg gggatgaaga
     1141 atctgtggga cagcgggctc cggcacgtgc tcgacggcca ttcgagcatc gaggagctgc
     1201 tccgggtggt ggacgtgccc acctcgctgc cgccggtgtc gcggccctcc gcggcgacgg
     1261 gatctccggc cgccgcggtc gagccctcgg ccgcggcggc agcgtcgccg gcggtcaccg
     1321 cgcccacggc cgacgtcgcg ctgagcttcg acctcatcga gcgcgagggc gcggccgccg
     1381 aaccgtcccg gcgggccgcg ggggccaaga tcctcctggt cgacgacgag gaccagctgc
     1441 gccggctcct caaggatctc ctcgagcgcg agggctacga ggtggtcgag gcccgcgatg
     1501 gcgccgaggc cctcgagcag gtcgatcggc acgggcccga cgtgatgctg ctcgacctga
     1561 acctccccgg gatggacggc tacagcgtcc tggcccacgt ccgctcccgc ccggccaccg
     1621 aggcgctccc gatcgtggtg ctcaccgcca agggcgacga ggacaacgaa gtgcgcgtcc
     1681 tccaggccgg cgccgacgat ttcctcacca agcccttccg cgcccgggcg ctgtatgccc
     1741 gcctcgaaaa gatcatggcg cgtcgcaagg cctgacgcgc atcccctcgt gtcagacatc
     1801 cgggtgacct acgtcgacgt ctacgtgctt cgcggcaccg gcgacgccgt cgaggcgctc
     1861 gtgctgcgcc ggagcagcgc ggggcgctgc cccggcgcgt gggagagcgt gcacggaggc
     1921 atcgagccgg gggagacgcc cgtgacggcg gcgctccggg agctccgtga ggagaccggc
     1981 ctcgtccccg gacggctcta caacctgagc cgggtcgagg cgttctaccg ccaccagcag
     2041 gacttcctgg cgctgatccc ggtgttcgtg gcgctggtac ccggcggggc ggaggtgcgt
     2101 ctttccgggg agcacgatgc cttcgactgg ctgccgccgc cggctgcggg ggcgcggatg
     2161 gcgtggccgc gggagcggcg ggcgctggag gatgcggtgg ccctcctgtc cgggggcgat
     2221 gcgggggcgg tcgaggacgt gctccgcgta tgctgacgcg cgccctcgtc cccgggctcc
     2281 tggcgtccgc gcttctcacc ggcgcaggat cgtccgcggc cccgtgcctc acgatccccc
     2341 gggcggggat gcgggtcgcg ggggacgtgc gcatctgccc cggcgcctac cgcgtacccg
     2401 accccgacga gaagggcgtg ctggtggtca cggcgtcggg gacgcgcatc gacctgaccg
     2461 gcgtcaccat ccggagcggc gactcgatcc cctcgcgcta cgtcggcatc ggtgtcgtga
     2521 gccgcggcaa cgacgaggtc gaggtggtcg ggggcgtcat cggcgggtat cgctacggca
     2581 tccggctcga gggggggcgg ggccaccgga tcagcggcgt cgacgtctcc ggctcgcggg
     2641 cccagccgct gcagtcgacg cccatccgct ttgccgatag cgactggctc gacatcttcg
     2701 acccggacac gttcgagacc tacggcgggg gcgtctacct caagtggacc gacggcgcca
     2761 cggtcaccgg cgtccaggcg cacggcgggc agaacgggat cgggctgttc ggctcgagca
     2821 acgggtacat cgccgacaac gacgtctcgg gcaacagcgg ctggggcatc cacctctggc
     2881 agtcgagcca caacgtcatc atccggaacg tggcccacac caacgtgcga tgcgaggcgg
     2941 ccgcgtacag ccggggctgc gactccgccg cgctcctcct gcgcaacgcc tgcgattcga
     3001 acctcatcgc ggacaacgac ctgcgccgtt ccggcgacgg gttcttcctg agcggccagc
     3061 cgccgacgat ggcgccgtcg gatgggaacg tcgtctccgg gaacgatggc tcctcctcgc
     3121 cccacaacgc cttcgagtcg acgttcagcg acggcaacac gttcctcgag aaccgcgccg
     3181 acagctccga ttacggctac tggctcgggt actcgagccg gacgctggtg cgcgggtcga
     3241 cggtggtggg gagccggagt gccgggatcg cgatcgagca cgggcgcgac aacgagctgc
     3301 gccagaacct gatcgtgggc gggaacgtcg ggatccggct ctttgcgccg gggggcgggc
     3361 cggtgccgag ccgtggcacc accgtggccg acaacacgat cgcccgggtg gggcgggggc
     3421 tcgtcctgga ggggaccacc gggtccagcg cgcttggcaa tgtgttcgac ggggttggga
     3481 cggcgctggt ggtcgactcg gcggggtgga acaccaccgt gcgggggaac gtcttcctcg
     3541 gcgcccgcga cgcctggatc gtggcgcctg accttgccgc cggcgacaac tactgggccg
     3601 cggccgacgt cgccgcgacg gtcgcccggg tgcggggcag gatcagcatc ctcccgtggc
     3661 acccggcgag tgctgcgggc tactgaccga atacctcctc ggccgcgctc cggagcgcac
     3721 gcaggtcgta ggggtgctgc acgacgaggt gccccgccgc ccggaaccgc tcggccatcc
     3781 ccggggccga gcgcggcacc gccaccacca ggcgccgccc gagctccggg tgcgcgtcca
     3841 gcagcaccgc caccagggtc ccgctgcccg gcgccggcat ccgcgcatcc accacgatga
     3901 ggtcgtaccg tcccgccgcc aggcgggcca gggcatcgtc cgccgaggtg gcggcctcca
     3961 cctcgtggcc gtcacgggcg aagagcgcgg ccacgagccg ttcattcttc gggtcgtcgt
     4021 cggcaaggag gatccggcgg ggcggccgcc cggccagcgc ctgggcctcg aggtgcggca
     4081 gcgtgatcga gaacgtcgcc ccgccccccg gcgcgggggc gtactcgagg cgtccgccgt
     4141 gggcctccac gatcccgtag ctgatcgaca ggccgagccc ggtgccctgg cccggttcct
     4201 tggtggtgaa gaacggcgtg aagaggagcg gcacgtgctc cggcgccacg cccgcgccgg
     4261 tgtcagtgac ccggaggacc acccggccgt cctgggccga cgtttcgagc ctcacgcgcc
     4321 cgtgctccgc tcccgtggtg gccaccgac
//
LOCUS       DASUAP010000330         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1184014, whole genome shotgun sequence.
ACCESSION   DASUAP010000330 DASUAP010000000
VERSION     DASUAP010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184014"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFU45_09010"
     CDS             <1..693
                     /locus_tag="VFU45_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359246.1"
                     /translation="LCVLLAQMGSYVPARSARIGIVDRLFTRVGASDNLARGQSTFMV
                     EMSETSAILHSATARSLVLLDEIGRGTSTYDGVAIAWAVSEHLHDRTGCKTMFATHYH
                     ELMQLPETLAHARNYNVAVRESGGTVVFLHRLEPGGTDQSYGVHVAELAGLPAPVVAR
                     AREVLMTLEGEHRVAPGAPPPPPDPGQLGLFAGEPDPVLQELGALDVDSLTPLEALNR
                     LAELKKRASRSK"
     gene            903..1313
                     /locus_tag="VFU45_09015"
     CDS             903..1313
                     /locus_tag="VFU45_09015"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU5359247.1"
                     /translation="MTPARILLLALVLSACRRAAPTTVQLPAAPANPPKAVEPPVAVN
                     ARSPVEYPQALYIQGIEGKVVLRLHVDSAGVVDPDSTSVAESSGYPALDSAALSAVPR
                     MKFAPALRNGVPSALTFTQPVIFKHAPRAGGVTP"
     gene            1310..2695
                     /locus_tag="VFU45_09020"
     CDS             1310..2695
                     /locus_tag="VFU45_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5359248.1"
                     /translation="MKPQHLEPYDSVLDTIGWTPLIRLARVGRGVRTPVFGKAEYVNP
                     GASVKDRIGLAIIEDAERRGELKAGGTIVEGTSGNTGVGLAIAAALKGYRCVFTLPDK
                     MSPEKIRLLKAYGAEVVVTPTAVPPDHPENYVMKAKQIAHDTPGAILANQFYNPVNPE
                     AHYRTTGPEVWEQTKGKVTHFVAGAGTGGTVSGTARYLKEKNPKIRIIAGDPVGSLYT
                     GYAKTKKLGDGAPYKVEGVGGDKAPTTVVWDLIDEWRQVSDRDSMAMARRLAREEGIL
                     VGGSAGLNVHIALEVARQLEDPNACVVTILCDTGERYLSKVFNDEWMQENGLLDVPRR
                     TVADLLARRPAAQPTLVSVTPASQVRQALNLMSTWGVSQLPVVEGQQVVGGLTEATLS
                     GKALAQPALLLQPVKEAMDPPFPVVDVATPLDHLATILSTTAPAAVVKQGDKLVGIVS
                     RHDLLQQLIGR"
     gene            2813..>3166
                     /locus_tag="VFU45_09025"
     CDS             2813..>3166
                     /locus_tag="VFU45_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU5359249.1"
                     /translation="MTHDPLHITVLTGGASAERDVALASATQVVAALRKRGHRVAVVD
                     TARGHVPEAEEPTLISGAVGHTPPSTAELVERERGFLVSSLAQIPAVRSADVLFLALH
                     GGWGEDGTLQAVLDVV"
BASE COUNT          496 a         1154 c         1085 g          431 t
ORIGIN      
        1 ctctgcgtcc tgctggcgca gatgggctcg tacgtgccgg cccggtcggc gcggatcggc
       61 atcgtcgacc ggctcttcac ccgggtcggc gccagcgaca acctggcgcg cggccagtcc
      121 actttcatgg tggagatgag cgagacgagc gccatcctcc acagcgccac cgcgcggagc
      181 ctggtgctgc tcgacgagat cggccgcggc acctcgacct acgacggcgt cgcgatcgcg
      241 tgggcggtct ccgagcacct gcacgaccgc accgggtgca agacgatgtt cgccacccac
      301 taccacgagc tgatgcagct gccggagacc ctggcgcacg cgcggaatta caacgtcgcg
      361 gtgcgggaga gcggggggac cgtggtcttc ctccaccgcc tcgagcccgg cggcaccgac
      421 cagagctacg gcgtccacgt ggcggagctc gccggactcc cggcgccggt cgtcgcgcgc
      481 gcgcgggagg tgctgatgac gctcgaggga gagcaccggg tggcgcccgg ggccccgccg
      541 ccgccgcccg atccggggca gctcgggctc ttcgccggcg agcccgaccc cgtcctccag
      601 gagctgggcg cgctcgacgt cgacagcctg acgccgctcg aggcgctcaa ccgcctggcc
      661 gagctcaaga agcgggcatc gcgatccaag taacgtggga ccacggagcg cacggaggga
      721 cacggagacg cacggagaac agcaggaata gtggtttcgc ggaccgggct ccgtctgcaa
      781 tgccaccact acagcagtcc tccgtgcctc tccgtggtcc caccttcgct cgcggtatgg
      841 ggcccgtgct cttgcgatct gcccgactcg ccccctacgc cgtttggcgc tagttttgcc
      901 gcatgacccc cgcccgcatc ctcttgcttg cgctcgtcct ttccgcctgc cgtcgcgccg
      961 cgcccaccac ggtccagctc cccgccgccc ccgccaaccc accgaaggcc gtcgagccgc
     1021 cggtggcggt gaacgcgcgg agcccggtcg agtaccccca ggcgctctac atccagggga
     1081 tcgaggggaa ggtggtcctc cgcctccacg tcgattccgc cggcgtcgtg gatcccgact
     1141 ccacctcggt ggccgagtcg agcggctatc cggcgctcga ttcggcggcg ctctcggcgg
     1201 tgccacggat gaagttcgcc cccgcgctcc gcaacggcgt cccatcggcg ctcaccttta
     1261 cccagcccgt catcttcaag cacgcaccac gcgccggagg cgtgaccccg tgaagcccca
     1321 gcacctcgag ccgtacgact ccgtcctcga caccatcggc tggacgccgc tcatccgcct
     1381 ggcccgcgtg gggcgcgggg tgcggacgcc ggtcttcggc aaggcggagt acgtgaatcc
     1441 gggcgcgtcg gtcaaggatc ggatcggcct cgccatcatc gaggacgcgg agaggcgcgg
     1501 tgagctcaag gcggggggca ccatcgtcga ggggacgtcg ggcaacaccg gcgtcgggct
     1561 cgcgatcgcc gccgcgctca aggggtatcg ctgcgtcttc acgctccccg acaagatgtc
     1621 gcccgagaag atccggctcc tcaaggcgta cggcgccgag gtcgtcgtga caccgaccgc
     1681 ggtgccgccg gaccatcccg agaactacgt gatgaaggcg aagcagatcg cccacgacac
     1741 ccccggggcc atcctcgcca accagttcta caacccggtg aaccccgagg cgcactaccg
     1801 caccaccggc cccgaggtct gggagcagac caaggggaag gtgacgcact tcgtggccgg
     1861 cgccggcacc ggcggcaccg tgagcgggac ggcgcggtac ctcaaggaga agaacccgaa
     1921 gatccgcatc atcgccggcg atccggtggg atcactctac accggctacg cgaagacgaa
     1981 gaagctgggg gacggcgcgc cctacaaggt ggaaggggtg ggcggcgaca aggcgccgac
     2041 gacggtcgtg tgggacctga tcgacgagtg gcgccaggtg tccgaccgcg actcgatggc
     2101 gatggcccgc cgcctggcgc gggaagaagg gatcctggtc ggcggatcgg cggggctcaa
     2161 cgtgcatatc gcgctcgagg tggcccgcca gctcgaggac ccgaacgcct gcgtcgtcac
     2221 catcctctgc gacaccggcg agcgctatct ctccaaggtc ttcaacgacg agtggatgca
     2281 ggagaacggg ctcctcgacg tgccccgccg caccgtggcc gatctcctgg cccggcgccc
     2341 cgccgcgcag ccgacgctcg tgtcggtcac gcccgcgagc caggtgcgcc aggcgctcaa
     2401 cctgatgagc acctgggggg tgagccagct cccggtggtc gagggacagc aggtcgtcgg
     2461 cgggctcacc gaggccacgc tctccggcaa ggcgctggcc cagccggcgc tcctgctgca
     2521 gccggtcaag gaagcgatgg acccgccctt cccggtggtc gacgtggcga cgcccctcga
     2581 ccacctggcc acgatccttt ccaccaccgc gcccgccgcg gtcgtgaagc agggcgacaa
     2641 gctcgttggc atcgtgagcc ggcacgacct gctgcaacag ctgatcggga ggtaaagcat
     2701 cgtgagacgt aagacgtgag acgtgacgga cgggcagcac tggtgacctg cgaccttcga
     2761 cccatgaccc gtgaccaatg acccacgacc cacgactcac gacccacgac tcatgaccca
     2821 cgacccgctc cacatcaccg tcctcaccgg cggcgcctcg gccgaacgcg atgtggcgct
     2881 cgcctcggcc acgcaggtcg ttgccgcgct tcgcaagcgg gggcaccggg tggcggtggt
     2941 ggacacggcg cgcgggcacg ttcccgaggc cgaggaaccg acgctcatct ccggggcggt
     3001 ggggcacacg cccccgtcga ccgccgagct ggtcgagcgc gagcgcgggt tcctggtgtc
     3061 gagcctggcg cagatcccgg cggtccggtc ggcggacgtc ctcttcctgg cgctgcacgg
     3121 cgggtggggc gaggacggca cgctgcaggc cgtgctcgac gtggtc
//
LOCUS       DASUAP010000331         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1047461, whole genome shotgun sequence.
ACCESSION   DASUAP010000331 DASUAP010000000
VERSION     DASUAP010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047461"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1717)
                     /locus_tag="VFU45_09030"
     CDS             complement(20..1717)
                     /locus_tag="VFU45_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5359250.1"
                     /translation="MDQNGADAMIVRMDRRACGALLLGLLLGAAHPLQAQVRRMTRKE
                     AKAVAKPVQMGVPALPDSSGWGVHVLAIARDANGGVWVGTYGNGIYVLPPNGTTWGHL
                     HSDTTGTSISFDFVNAIAFGQRGEIWYGTVGNGWGLSTDNGHTWKNWTLRQLGPEWQY
                     VAPDGILVRGDTVYIATADGIQVTTDDGGHWQALEDAVGPLDKGPADSAFAVLPSEYV
                     RRISTDRRGVVVSTLRGVVRLDHGPGGWIATPASYVSFRPINSLLLNDRLIRGTQCGL
                     RFSSDTMPCFTRATVVADSGLDLRTTWFQRPISVVDDPFIDQTYRYGSTMGGTFQPHQ
                     GVEFNNPDGTPVHAIGSGVVVYSGPAEAGANTVAIRMDSTITGSVPTSPEDGPPGPPV
                     KLHLFSVYYHNTRLGVHVGERVKAGDVIAWVGNTGRATNDHVHLEVHASPTDSVPAIV
                     DSLNRYPPYTTNPELWIEPLDKTGVVAGQVWDTAGHAVPQARIYGLVKPEPIETPFSY
                     IETYGPKNHSHPLYHEHFAISDVPPGDYVMGVAIDGKRVFRKVTVVAGKLAWVVFRP"
     gene            complement(1690..2376)
                     /locus_tag="VFU45_09035"
     CDS             complement(1690..2376)
                     /locus_tag="VFU45_09035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5359251.1"
                     /translation="MSRPLAVVTGASRGIGAAAAERLRAAGWDVARLARSLADRDADG
                     FHDRRCDVTDPVAVGRVAAALAPLGVPDVLVNNAGHFLMRRFDEISPAEFAAEVALNL
                     TAAFTVAHTFLPAMMRAGRGTLVTIGSVADHVGFPGNAAYGASKFGLRGMHESLAAEC
                     RGTGVRCTLVSPGPTDTAIWDDVRPGPRGGLPSREEMMSPGDVADAVVFAATRPANVR
                     VEWIRMEPMR"
     gene            complement(2373..2948)
                     /gene="folE"
                     /locus_tag="VFU45_09040"
     CDS             complement(2373..2948)
                     /gene="folE"
                     /locus_tag="VFU45_09040"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU5359252.1"
                     /translation="MAAEPGFQSHVRQMLEALGEDPGRAGLRKTPERVEAAMRWLTRG
                     YEMSVAQVVGDAVFEEQHESMILVRDIELYSLCEHHLLPFFGRAHVAYIPDGRIVGLS
                     KLPRIVEVFSRRLQVQERLTDQIADAVMDVLRPQGVGVVIEALHLCMAMRGVEKQNSK
                     TVTSALRGVFRDDPRTREEFLRLVQGGGPIL"
BASE COUNT          451 a         1110 c         1095 g          458 t
ORIGIN      
        1 aaggcgcgcg acatcgccgt cagggccgga agacgaccca cgccagcttg ccggcgacca
       61 cggtcacctt ccggaagacc cgcttcccgt cgatcgcgac gcccatcacg tagtcgccgg
      121 gtggcacgtc ggagatggca aagtgctcgt ggtagagcgg atggctgtgg ttcttcgggc
      181 cgtaggtctc gatgtaactg aagggcgtct cgatcggctc gggcttgacc aggccgtaga
      241 tccgcgcctg gggcacggcg tggcccgccg tgtcccacac ctggcccgcg acgacgccgg
      301 tcttgtccag cggctcgatc cagagctcgg gattggtggt gtagggcgga taccgattca
      361 gcgaatccac gatggccggg accgaatcgg tcgggctcgc gtgcacctcg agatggacgt
      421 ggtcgttggt ggcgcgcccg gtgttgccga cccacgcgat cacatcgccg gccttgaccc
      481 gctcgccgac gtggacgcca aggcgggtgt tgtggtagta gaccgagaag aggtgcagct
      541 tgaccggcgg cccgggcggc ccgtcctcgg gcgaggtggg caccgacccc gtgatcgtgg
      601 agtccatccg gatggccacc gtgttggccc cggcctcggc cggccccgag tacaccacca
      661 ctccgctccc gatcgcgtgc accggcgtgc cgtcggggtt gttgaactcc acgccctggt
      721 ggggctggaa ggtgccgccc atcgtggagc cgtagcggta ggtctggtcg atgaacgggt
      781 catcgaccac cgagatcggg cgctggaacc aggtggtgcg gaggtcgagc cccgaatcgg
      841 ccacgacggt ggcgcgggtg aagcacggca tggtgtcgga gctgaaccgg agcccgcact
      901 gggtcccgcg gatcagccgg tcgttcagga gcaggctgtt gatcggccgg aacgagacgt
      961 agctcgccgg ggtcgcgatc catccgccgg gcccgtggtc gagccgcacc acgccgcgga
     1021 gggtggacac cacgaccccg cgacggtcgg tgctgatccg gcgcacgtat tcgctcggca
     1081 gcaccgcgaa ggccgaatcc gccgggccct tgtcgagcgg ccccaccgcg tcctccagcg
     1141 cctgccagtg gccgccatcg tcggtggtca cctggatgcc gtcggcggtg gcgatgtaca
     1201 ccgtgtcgcc ccggaccagg atgccgtcgg gcgccacgta ctgccactcc gggccgagct
     1261 gcctgagcgt ccagttcttc caggtgtggc cgttgtcggt cgagaggccc cagccgttcc
     1321 ccaccgtccc gtaccagatc tcgccgcgct ggccgaaggc gatggcgttg acgaagtcga
     1381 acgagatcga ggtccccgtg gtgtcgctgt ggaggtggcc ccacgtggtc ccgttcggcg
     1441 gcaggacgta gatgccgttc ccgtaggtgc ccacccagac cccgccgttg gcgtcccgcg
     1501 cgatggccag gacgtggaca ccccagccgc tcgagtcggg gagcgcgggc acgcccatct
     1561 ggaccggctt ggcgacggcc ttggcctctt tccgcgtcat tcggcgcacc tgcgcctgca
     1621 gcgggtgcgc ggctcccagg aggaggccca gcagcagtgc accgcacgcc cgtcgatcca
     1681 tcctgacgat catcgcatcg gctccattct gatccattcc acccgcacgt tcgccgggcg
     1741 ggtggccgcg aacacgaccg cgtccgccac gtcccccggc gacatcatct cctcccgcga
     1801 cggcagcccg ccgcggggcc ccggccgcac gtcatcccag atggccgtgt cggtgggccc
     1861 cggcgagacg agggtgcagc ggacgccggt gccccggcat tcggctgcca gcgactcgtg
     1921 catcccccgg aggccgaact tgctggcgcc gtacgccgcg ttccccggga acccgacgtg
     1981 gtcggcgacg ctcccgatcg tcaccagcgt gccccgcccc gcccgcatca ttgccggcag
     2041 gaaggtgtgc gccaccgtga acgccgcggt caggttgagc gccacttcgg ccgcgaactc
     2101 cgccggggag atctcgtcga accggcgcat caggaaatga ccggcgttgt tcaccagcac
     2161 gtcgggcacg ccgagcgggg cgagcgccgc cgccacccgg ccgaccgcga ccgggtccgt
     2221 cacgtcgcat cgccggtcgt ggaatccatc ggcgtcgcgg tcggcgagcg accgcgccag
     2281 gcgcgccacg tcccagcctg ccgcacggag gcgctcggcc gccgccgcgc cgatcccgcg
     2341 cgacgcgccg gtgacgacgg cgagaggccg gctcacagga tcggcccgcc gccctggacc
     2401 aggcggagga attcctcgcg cgtgcggggg tcgtcgcgga agacgccgcg gagcgcgctc
     2461 gtgaccgtct tgctgttctg cttctcgacg ccgcgcatcg ccatgcacag atggagcgcc
     2521 tcgatcacga cgccgacgcc ctgcggccgg agcacgtcca tcacggcatc ggcgatctgg
     2581 tcggtcaggc gctcctggac ctggagccgg cgggaaaaga cctcgacgat gcgcggcagc
     2641 ttggacaacc cgacgatgcg gccgtccggg atataggcca cgtgcgcccg gccgaagaac
     2701 gggagcaggt ggtgctcgca cagcgagtag agctcgatgt cccgcacgag gatcatgctc
     2761 tcgtgctgct cctcgaacac cgcgtccccc accacctggg ccaccgacat ctcgtagccg
     2821 cgggtgagcc agcgcatcgc cgcctccacc cgttcggggg tcttccggag ccccgcgcgg
     2881 ccgggatcct cccccagcgc ctcgagcatc tggcggacgt gggactggaa gccgggttca
     2941 gcggccatcg tattcgacat agttgcgggg ggtctcccag aggcggaccc ggagcgcgag
     3001 cgcggccggg atgcgcggcc gcagttcccg ccacgccacc agcgcgatgt tctctgccga
     3061 gggcaggagg tcgcggaacc aggccacatc gaggttcagg ttcttgtgat cgag
//
LOCUS       DASUAP010000332         4511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1047500, whole genome shotgun sequence.
ACCESSION   DASUAP010000332 DASUAP010000000
VERSION     DASUAP010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047500"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VFU45_09045"
     CDS             <1..1003
                     /locus_tag="VFU45_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5359253.1"
                     /translation="KGDHEQYFTGYFSERGFRDQLGQVLNCSGKDVALCQNATIGMNF
                     VANGLELAAGDEVVVTDQAHPGGRKGYDLRAKRDGIVVKEVKVPDPVASPDQLIDGFL
                     GLTTPKTKVWAIPHISSGRAIRYPVEKLCALARERGIFSAVDGAQALGHLAIDVQAMG
                     CDAYFGSPHKWLLAPKGTGFLYVRPERQKGLWATLASFAWDDQDDRMFALMQYGTGNL
                     SLLKGLGKAIEFHLELGPKRVQDRILGLADRLRTGLQAIPGVAFWSPLHPELTCASTV
                     YGLKGTTGDAIQDYLWDKAKIRVRSEGPAGVRHCCHIYNSPGDVDRAVALLRSMTRT"
     gene            1260..1730
                     /locus_tag="VFU45_09050"
     CDS             1260..1730
                     /locus_tag="VFU45_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEU5359254.1"
                     /translation="MIREMRHKLGKEIEELSHELTVTLPQAIATAVAMGDLRENSEYK
                     AALERQQFVQARLGQLHQRLNQLHQLASVETPANEVGLGSTVTVVDIKTKEKEVFVLV
                     LGEMADVDEGHITLASPLGRALKGRKVGDEVELVLPTMRRQLKVVGLEAVGKES"
     gene            1923..2291
                     /locus_tag="VFU45_09055"
     CDS             1923..2291
                     /locus_tag="VFU45_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HEU5359255.1"
                     /translation="MDFPKKQLLVVGDRVLITPEEGEERTRVGLYLPATAIDAQQVQS
                     GTVIGTGPGEPIDDLSALDAEPWKLGAKDQRYRPMQARIGDHAIFFRKAAVEITFEEK
                     RYLVVPLAAILVLVRQDLPI"
     gene            2416..2721
                     /locus_tag="VFU45_09060"
     CDS             2416..2721
                     /locus_tag="VFU45_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359256.1"
                     /translation="MPFELPPAARQYRAAMWLFALLAIAAVVPVVQGIRGVIPRTLGL
                     ASGGLSVAGFFLAATFGLRGRRLVIEAREREAARLALVMLAGVLKDRGDDELEALAR"
     gene            2814..3356
                     /locus_tag="VFU45_09065"
     CDS             2814..3356
                     /locus_tag="VFU45_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359257.1"
                     /translation="MCYLLTVASPLTLSEVRSMLPAGMHADLQEAALVHAWRTILPET
                     QAVAALIHGGCSCDLVLPRPADAKADDAELRKRYRVAGASRAVTIHALERHAKARTLR
                     KYAPGHWPVAFNRFVAEHARNAGETGYLLEYETGAEAQERSDAERPLRPSATAPLRLC
                     VPARNDHAWLVPDRVIVITP"
     gene            3421..>4511
                     /locus_tag="VFU45_09070"
     CDS             3421..>4511
                     /locus_tag="VFU45_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5359258.1"
                     /translation="MTSESAGALPPEPNEPEPAGEEPSPLRRLKHALFGRPRDLTDTG
                     LFHRLALIPFLAWVGLGADGLSSSSYGPEEAFRALGQHTYLAIGLALVTAFTVLLISA
                     AYSRLIEEFPTGGGGYVVATKLLGPSAGVISGSALLVDYVLTITTSLAAAGDALFSLL
                     GPQWVPLKLPLEVVLIVVLTGLNIRGVRESVIALLPIFLLFLVTHVLVIGGGIVAHLG
                     RVPEVVQQVQTGYSSGMSLLGAVGMTLLFVRAYSLGGGTYTGIEAVSNGLPIMRDPKV
                     RTGKRTMLYMATSLAFTAGGLIVCYLLWGIGPVEGKTMNAVLVERMVGGMPFGHLFLI
                     LTLFSEGALLVVAAQAGFIDGPRVLANMAV"
BASE COUNT          681 a         1671 c         1470 g          689 t
ORIGIN      
        1 caagggcgac cacgagcaat acttcaccgg ctacttcagc gaacgcggct tccgcgacca
       61 gctgggccag gtgctcaact gcagcgggaa ggacgtggcg ctctgccaga acgccacgat
      121 cgggatgaac ttcgtcgcca acgggctcga gctcgccgcc ggcgacgagg tcgtcgtcac
      181 cgaccaggcc caccccggcg gccgcaaggg atacgacctc cgcgccaagc gcgacggcat
      241 cgtcgtgaag gaagtgaagg tccccgaccc cgtcgcttcc cccgatcagc tgatcgacgg
      301 attcctcggt ctcaccacgc cgaagaccaa ggtctgggcc atcccccaca tctcctccgg
      361 ccgcgccatc cgctacccgg tggaaaagct ctgcgccctg gcgcgcgagc gcggcatctt
      421 cagcgccgtc gacggcgccc aggccctcgg ccacctcgcc atcgacgtgc aggccatggg
      481 ctgcgacgcc tacttcggct ccccccacaa gtggctgctg gcgccgaaag gcaccggatt
      541 cctctacgta cgtccggaac gccagaaggg actctgggcc accctcgctt cctttgcctg
      601 ggacgaccag gacgaccgga tgttcgccct gatgcagtac ggcaccggca acctgagcct
      661 cctcaaggga ctgggaaagg cgatcgagtt ccacctcgag ctcggcccga agcgggtgca
      721 ggaccggatc ctcggcctcg ctgaccggct ccggaccggg ctccaggcca tcccgggcgt
      781 cgccttctgg tcgccgctcc accccgagct cacctgcgcg tcgacggtct atggcctgaa
      841 ggggaccacc ggcgacgcca tccaggacta cctctgggac aaggccaaga tccgcgtccg
      901 cagcgaaggc ccggccggcg tccgccactg ctgccacatc tacaactcgc ccggggacgt
      961 ggaccgggcg gtggcgctgc tgcggtcgat gacacggaca taggtgcgag tggcgagtgg
     1021 cgagtgagtg gctagtggcg ggtggcgagt gagtggcagg cgagcgatgc gagtcgcccc
     1081 ccgctcgtag cccgcaaccc gcaacccgcc acccgccacc cacccgtcac tcgccactcg
     1141 ctacccgtac ctccccctat cgcccccgcg gggcggggtt ataatagagg cagcaaccag
     1201 cgatcccggg ccggcagatg cccgggatcg ttccctttta ctgtcaacgg actattccga
     1261 tgatccgtga gatgcgccac aagctcggca aggagatcga agagctctcc cacgagctca
     1321 ccgtgaccct gccgcaggcc atcgccaccg ccgtggccat gggcgacctc cgcgagaact
     1381 ccgagtacaa ggcggcgctg gagcgccagc agttcgtcca ggcgcgcctg ggccagctcc
     1441 accagcgcct gaaccagctg caccagctcg cgtcggtcga gacccccgcc aacgaagtgg
     1501 ggctcggctc cacggtcacc gtcgtggaca tcaagaccaa ggagaaggag gtcttcgtcc
     1561 tggtgctggg cgagatggcc gacgtcgacg aaggccacat caccctcgcc tccccgctcg
     1621 gccgcgccct caagggccgc aaggtgggcg acgaggtcga actcgtcctc cccaccatgc
     1681 gccggcaact caaggtggtg gggctggaag cggtggggaa agagagctga gagctgagag
     1741 ctgagagctt ggagctgaga gcttggagct tagagcgtgg agcttggagt tctcgaccga
     1801 tgactgttcg atctcgttgc ggcgcattcg gctgaagtcc ttggactctc agccctaagc
     1861 tctaagccct aaaccctaag ctctagactc tcagctctag actcccagct ccaggctccc
     1921 acatggactt ccccaagaag cagctcctcg tcgtcggcga ccgggtcctc atcacccccg
     1981 aagagggcga ggagcgcacc cgcgtggggc tctacctccc tgccacggcc atcgacgccc
     2041 agcaggtcca gagcggcacg gtcatcggca ccggtcccgg cgagccgatc gacgacctct
     2101 ccgccctcga cgccgagccc tggaagctcg gcgccaagga ccagcgctac cgcccgatgc
     2161 aggcccggat cggcgaccac gccatcttct tccgcaaggc cgccgtcgag atcaccttcg
     2221 aggagaagcg ctacctcgtc gtccccctcg ccgcgatcct ggtgctggtg cggcaggatc
     2281 tgccgatcta gccacgggct gcgtgaggcg ggggacgggt gggccacgcg cggcaggcgg
     2341 cgaacaggcc tgcccgacgc ccgctgcccg tcgccagcgc ggcacgcgtc tcccgtctct
     2401 cgacccctga cgaccatgcc attcgaactc ccccccgccg cccgccagta ccgcgccgcg
     2461 atgtggctct tcgcgctcct cgccattgcg gctgtggtgc cggtggtcca ggggatccgc
     2521 ggggtcatcc cgcgcacgct gggactcgca tcggggggcc tcagcgtggc cgggttcttc
     2581 ctcgcggcca ccttcggact ccggggccgg cgcctggtca tcgaggcgcg ggagcgggag
     2641 gcggcgcgac tcgcgctcgt gatgctcgcc ggggtgctca aggaccgtgg cgacgatgag
     2701 ctcgaggccc tggcccggta ggacggctcc gcgggcgagg ccgcccggtc agtgctcgac
     2761 cgccgccgcg aggaccggga acgcgccgcc cggcgcgccg ccccgcccgc ctgatgtgct
     2821 acctcctgac ggtggcgtcg ccgctcacgc tgagcgaggt gcgctcgatg ctccctgccg
     2881 ggatgcacgc cgacctgcag gaggcggccc tggtccacgc ttggcgtacc atcctcccgg
     2941 agacccaggc ggtggccgcg ctcatccacg gcggctgttc ctgcgacttg gtcctgcccc
     3001 gccccgccga tgccaaggcc gatgacgccg agctccgcaa gcgctaccgg gtggccggag
     3061 cctcccgggc cgtcaccatc cacgccctgg agcgccacgc caaggcccgc acgctccgga
     3121 aatacgctcc cgggcactgg ccggtggcct tcaaccggtt cgtcgccgag cacgcgcgga
     3181 acgcggggga aacggggtac ctgctggagt atgagacagg cgcagaggcg caggagcgca
     3241 gtgacgcaga acgacccctg cggccctccg ccactgcgcc gctgcgtctc tgcgtccctg
     3301 cgcgcaacga ccacgcctgg ctcgttccgg accgggtgat cgtcatcacg ccgtagcggt
     3361 cccggcgcgc cggccggcgc gtgctatatt cgttccccgg cagtctctgc cgcggagcca
     3421 atgacatccg aatcagccgg cgcattgccg ccggaaccga acgaacctga gccggccggc
     3481 gaggagcctt ccccgctccg ccggctcaag cacgcgctct tcggccgccc ccgcgacctc
     3541 accgacaccg gccttttcca ccggctggcg ctcatcccct tcctggcctg ggtggggctc
     3601 ggcgcggacg ggctgtcatc ctcctcctac ggccccgagg aggccttccg ggcgctgggc
     3661 cagcacacct acctggccat cggcctggcg ctggtcacgg cgttcaccgt actgctcatc
     3721 tccgccgcgt acagccggct gatcgaggag ttccccaccg gcggcggcgg gtacgtggtg
     3781 gccaccaagc tcctggggcc gagcgccggc gtcatctcgg ggagcgcgct gctcgtcgac
     3841 tacgtcctca ccatcaccac ctcgctcgcc gccgccggcg acgccctctt cagcctcctc
     3901 ggtccccagt gggtgccgct caagctcccg ctcgaggtgg tgctcatcgt ggtgctcacc
     3961 gggctcaaca tccgcggcgt gcgggagtcg gtcatcgcgc tgctcccgat cttcctgctc
     4021 ttcctcgtca cccacgtgct cgtgatcggc ggcgggatcg tggcgcacct gggccgcgtg
     4081 ccggaagtgg tccagcaggt gcagaccggc tactcgagcg ggatgtcgct cctgggtgcg
     4141 gtagggatga cgctcctgtt cgtccgggcc tactcgctgg gtggcggcac ctacaccggg
     4201 atcgaggccg tgtccaacgg cctccccatc atgcgcgatc ccaaggtccg gaccgggaag
     4261 cgcacgatgc tctacatggc cacctcgctc gccttcaccg ccggcggcct gatcgtctgc
     4321 tacctgctct ggggcatcgg gccggtcgag ggaaagacga tgaacgcggt gctggtggag
     4381 cggatggtgg gagggatgcc cttcggccac ctcttcctca tcctgacgct cttttccgag
     4441 ggcgcgctcc tggtggtggc ggcgcaggcg gggttcattg acgggccccg ggtgctggcc
     4501 aacatggccg t
//
LOCUS       DASUAP010000333         3797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_665848, whole genome shotgun sequence.
ACCESSION   DASUAP010000333 DASUAP010000000
VERSION     DASUAP010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665848"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2974)
                     /locus_tag="VFU45_09075"
     CDS             complement(<1..2974)
                     /locus_tag="VFU45_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359259.1"
                     /translation="MSCRVRGSVLALLLAAPLAGQAPAPKPWPVVPVPYVEGYAETIA
                     GQRFTYRTADPLITSSLLVRSVDSSRYIEWRSAPVPAAAQGPTVTFQWMASMDVEDPG
                     TPQRHFVLSLNGRPWFTIPQPASATPADWGVDGPDGARLSFHTLLVDWAGDVNGLMSM
                     RVPLADFPTGAAVTMRVTGESAGSQAWYMTFTQPVAPMAQVSAEQALVRTPSGVRQAL
                     RVGIWSLADSTPVTVSVDGSTRLRTMIVRGWNIRHVPIPQVTRPIDERVTLASDWDSV
                     SWDRLHLEPVVSRTIYLIPHAHLDIGYNVLQAEAGRRHEATYDEGMALARKTAAYPAD
                     ARFHWNVEGLWPVEDYLTTRPAKERAAFLAAARRGDLSLNAFYANLMTGLSRPEELGE
                     AVRYAATLRSRYHIPINSAITSDVPGFSWGVVPALARAGIRYLSSGPNYQSTLPGRGD
                     RIGNLLKAWGDSAFYWMSPSGTDSVLVMVAGRGYSWFHGRRRGWPGLADDGGLIDYMD
                     SLSADHYPWDIVQVRYTTGDNGGVDTLLPDAVRRWNRTYASPRVVIGTLPQMFSAFER
                     RYGSKLQRVRGDLTGYWEDGAGSTARETAESRMAAERLTEAATLFAMRAPAAYDAGAF
                     WQAWRRVLLYSEHTWGADRSISDPDDPFVLEQWAVKRGFADSAAARADALLYRAAGGP
                     APAGVINALDVFNPTSSTRSAIAGLPAGINATRDDVTDAAGRIVPVERLSNGTLAFLA
                     DSIPAFGARRFWFYPGWARAAGDAHAAGDSLWNSTIVARVDTVTGALASVRWRGHELV
                     DRAHGGWNEYENVAGQSSSAAQGVTGVHVAVAEAGPLLARIRVTSAAPGARSLTRTYS
                     LGAGSDFLELTTTIDKAPVRSKEGVHIGFPFLVPGGQVRLDIPWAIVRPDSDQLRGAD
                     RNVFTVDRWADVSNDSIGVTWATLDAPLVEVGGMWGEDWHHDDGTEGWLTHLPATQLL
                     YSYVMNNYWHTNFKADQPG"
     gene            complement(3149..>3797)
                     /locus_tag="VFU45_09080"
     CDS             complement(3149..>3797)
                     /locus_tag="VFU45_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU5359260.1"
                     /translation="KEVEFAGYFGRTAAQVRKTLDQNGLVSPSSHIAIEDLEKDPNAI
                     FDYARTVGHEWVVVAWLDEDRRNTVAALQRVADEFNTIGEKAKAAGLRFAYHNHNPEF
                     TPIEGQLPYDIFLSRTDPKLVQFEVDLYWIVNGGGDPLKYFAAYPGRFPMVHVKDMTK
                     DRKMVDVGQGSIDWKGIFAHAKQAGIEHFFVEHDDPPTPLADIKVSFDYLRRLRF"
BASE COUNT          584 a         1307 c         1312 g          594 t
ORIGIN      
        1 tccccggctg gtcggctttg aagttggtgt gccagtaatt gttcatcaca taggaataga
       61 ggagctgggt cgcgggcagg tgcgtgagcc agccctccgt tccgtcgtcg tggtgccagt
      121 cctcccccca catcccgccc acttcgacga gcggcgcatc gagcgtcgcc cacgtcaccc
      181 cgatgctgtc gttggagaca tcggcccagc ggtccacggt gaacacgttg cggtcggctc
      241 cccggagctg gtcgctgtca ggccggacga tggcccacgg gatgtcgagc cgcacctggc
      301 cgccgggcac caggaagggg aagccgatgt ggaccccttc cttcgagcgc accggcgcct
      361 tgtcgatggt ggtggtgagc tccaggaaat cggatccggc gccgagtgag taggtgcggg
      421 tgagcgatcg ggcgccaggg gcggcggaag tgaccctgat cctggccagc agcggccctg
      481 cttcggcgac ggcaacgtgc acgccggtca caccctgggc ggcgctggat gactgtcccg
      541 ccacgttttc gtattcgttc catccgccgt gcgcgcggtc caccagttcg tgcccgcgcc
      601 accgcacgct cgcgagcgcg ccggtgacag tgtccacccg cgccacgatt gtcgagttcc
      661 agagggaatc acccgcggcg tgcgcgtcgc cggcggcgcg ggcccaacct ggatagaacc
      721 agaaccgtcg ggcgccgaag gccgggatgg aatccgccag gaaggcgagc gtgccgttgg
      781 acagccgctc gacgggaacg atccgcccgg cggcgtcggt cacgtcgtca cgcgtcgcgt
      841 tgatccccgc ggggagcccg gcaatcgccg agcgcgtgct cgacgtcgga ttgaatacgt
      901 cgagtgcgtt gatcactccc gccggggcgg gaccgcccgc tgcgcggtag aggagcgcat
      961 cggcccgggc ggcggcggag tcggcaaagc cgcgcttcac cgcccactgc tcgagcacga
     1021 acggatcgtc ggggtcgctg atgctccggt cggcgcccca ggtgtgctcg ctgtagagca
     1081 acacccggcg ccacgcctgc cagaacgcgc cggcatcgta ggccgccggt gcgcgcatcg
     1141 caaagagtgt cgccgcttcg gtcagccgct ccgccgccat cctgctctcg gcggtctccc
     1201 gagcggtgga ccctgcgccg tcctcccagt acccggtcag gtcgccacgg acccgctgga
     1261 gcttcgaccc gtaccgccgt tcgaacgcgg agaacatctg cggcagggta ccgatgacga
     1321 cgcggggcga ggcgtaggtc cggttccagc ggcgaacggc gtcgggcagc agcgtgtcga
     1381 cgccgccgtt gtcgccggtg gtgtagcgca cctgcacgat gtcccacggg taatggtcgg
     1441 ccgagaggga atccatgtag tcgatcaggc caccgtcgtc cgcgagcccg ggccatccgc
     1501 gccggcggcc gtggaaccag gagtacccgc gccccgccac catcaccagc accgaatcgg
     1561 tccccgacgg cgacatccag tagaacgcgc tgtcccccca cgccttgagg aggttgccaa
     1621 tccggtcgcc gcggccggga agggtggact gatagttggg tccgctcgag aggtatcgga
     1681 tgccggcacg cgccagcgct ggcaccacgc cccagctgaa cccggggaca tccgatgtga
     1741 tggccgagtt gatcgggatg tggtagcggg agcggagggt ggccgcatag cggaccgcct
     1801 cccccagctc ctcggggcgg gagaggcccg tcatcaggtt ggcatagaat gcgttgagcg
     1861 agaggtcgcc gcgccgagcg gcggccagga acgcggcgcg ctccttcgcg gggcgggtgg
     1921 tcaggtagtc ctccaccggc cagagtcctt cgacgttcca gtggaagcgc gcatcggcgg
     1981 gatacgccgc ggtcttccgg gccagcgcca tcccctcgtc gtacgtggcc tcgtgtcggc
     2041 gtcccgcctc cgcctgcaag acgttgtacc cgatgtcgag gtgcgcgtgc ggaatgagat
     2101 agatggtcct cgacaccacc ggctccaggt gcaggcggtc ccagctgacc gagtcccagt
     2161 ccgaggcgag cgtcacccgt tcgtcgatcg gccgagtcac ctgcgggatc gggacgtggc
     2221 ggatattcca cccgcgcacg atcatggtcc ggagccgggt cgacccgtcg acgctcaccg
     2281 tcaccggcgt gctgtcggcc aggctccaga tgcccacccg gagcgcctgc cgcacccccg
     2341 acggcgtccg caccagcgcc tgctccgccg agacctgcgc catcggcgcc accggctggg
     2401 tgaacgtcat gtaccacgcc tggctcccgg cgctctcacc ggtgacccgc atcgtgactg
     2461 cggcgccggt ggggaagtcg gcaaggggca cccgcatcga catcaggccg ttgacgtcgc
     2521 ccgcccagtc gacgagaagg gtgtggaatg agaggcgcgc gccatcgggg ccgtcgacgc
     2581 cccagtccgc cggcgtcgcc gaggccggct gggggatggt gaaccagggg cgcccgttga
     2641 gcgaaagcac gaagtgacgt tgcggggtcc cggggtcctc cacgtccatc gacgccatcc
     2701 actggaacgt caccgtcggg ccctgtgcgg cggcggggac cggtgccgag cgccactcga
     2761 tgtagcggga gctgtcgacg gaacggacca ggagggagga ggtgatgagg ggatcggccg
     2821 tgcggtaggt gaagcgctgc cccgcgatcg tctccgcgta tccctcgacg tacggcaccg
     2881 gcaccaccgg ccacggcttc ggtgccggcg cctggccggc gaggggtgcg gccaggagca
     2941 gcgccaagac cgatccgcgg acgcggcagc tcacgtacca ggctcctctc cgcaaagtgg
     3001 gaccacggag tacacgaagg gctcggggat acacggaggc ctgccgttga tgtggtctcg
     3061 cgaacggaac ctcgtcgatc ccgccacacc acgatgcagt tctccgtgcg tctccgtgtg
     3121 cctccgtgca ctccgtggtc ccacgttatc agaacctcag ccgacggaga tagtcgaagc
     3181 tcaccttgat atccgccagc ggcgtcggcg ggtcgtcgtg ctccacgaag aagtgctcga
     3241 tccccgcctg cttcgcgtgt gcgaagatcc ccttccagtc gatgctcccc tggcccacgt
     3301 ccaccatctt ccggtccttg gtcatgtcct tgacgtgcac catcgggaag cggccgggat
     3361 atgcggcgaa atacttgagc ggatcgccgc cgccgttcac gatccagtag aggtccacct
     3421 cgaactggac cagcttgggg tcggtgcggg agaggaagat gtcatagggg agctggcctt
     3481 cgatcggcgt gaactcgggg ttgtggttgt ggtaggcgaa ccggagcccc gccgccttcg
     3541 ccttctcgcc gatcgtgttg aactcatccg ccacccgctg cagcgcggcc acggtgttgc
     3601 ggcggtcctc gtcgagccag gccacgacca cccactcgtg ccccaccgtg cgcgcgtagt
     3661 cgaagatcgc gttcggatcc ttctccaggt cctcgatcgc gatgtgagat gacggcgaca
     3721 cgagcccgtt ctggtcgagc gtcttccgca cctgcgccgc cgtccgcccg aagtaaccgg
     3781 cgaactcgac ctccttg
//
LOCUS       DASUAP010000334         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_270714, whole genome shotgun sequence.
ACCESSION   DASUAP010000334 DASUAP010000000
VERSION     DASUAP010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270714"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..690)
                     /locus_tag="VFU45_09085"
     CDS             complement(4..690)
                     /locus_tag="VFU45_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU5359261.1"
                     /translation="MHGESHRGDRIGWLRAAVLGANDGLISTSSLVVGVAAADPSRSA
                     VLLAAVAGLAAGALSMAAGEYVSVSSQADTEHADLARERRELAAAPEAERRELADIYV
                     KRGLDRELAIRVADQLMAHDALGAHARDELGIHEITRARPIQAAVASAASFAVGAAPP
                     AFLAAFWAIRGLSAAIVVLTLALLLVLGAVAARLGGASLTRGAVRVAFWGAVAMACTA
                     LVGRLFGAVV"
     gene            complement(680..985)
                     /locus_tag="VFU45_09090"
     CDS             complement(680..985)
                     /locus_tag="VFU45_09090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEU5359262.1"
                     /translation="MYGLIAKLTATPGQRDALAAILLEGSGAMPGCLSYVIARDTADH
                     DALWVTEVWESAASHQASLSLPSVKAAIAKGRPLIAGSSHRVETAPVGGQGLARHAR"
     gene            complement(1122..1418)
                     /locus_tag="VFU45_09095"
     CDS             complement(1122..1418)
                     /locus_tag="VFU45_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5359263.1"
                     /translation="MPTYETKGPFCQSCSMPLNRPEDFGTDQAGFRINDYCTHCYANG
                     AFTDPGISMQDMLDRSVDAMDQAGVMPAPQARALLLDVLPRLKRWRAPSAAMSR"
     gene            complement(1529..2356)
                     /locus_tag="VFU45_09100"
     CDS             complement(1529..2356)
                     /locus_tag="VFU45_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5359264.1"
                     /translation="MAAATGARAQVVRVWPGTAPGSEHWTQQEKTYTGTPLGTVVIDV
                     VTPTLTAYLPARSTATGTGVIIAPGGAFVALALDLEGTDAARWLQRRGIAAFVLKYRT
                     VEKKQDGIPEMDFDTAGRYGIADGIQALKVVRAHAAEWGVLPDRIGILGFSAGGMVAS
                     GALLQPDSAARPDFAAMIYGGPLGAMPAIPPHLPPTFLAWAQDDRLAGDAVARFYAAL
                     MAAGDRPEAHIYGSGGHGFGLRHQGTTSDHWIEQFYQWLEAGGLTRRAARHPRHRPS"
     gene            complement(2418..2852)
                     /locus_tag="VFU45_09105"
     CDS             complement(2418..2852)
                     /locus_tag="VFU45_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5359265.1"
                     /translation="MATLRDLLTRKGSEVISVQPTDTVLHVAELMNTRNVGGVAVLDG
                     GKLAGIFTERDVLRRVVARGLDPATTRVSAVMTADVITCPPDLDVEECAALMTARRVR
                     HLPVEEGGALKGIVTIGDLLAHQVREQQATLEHMHNYLHDLR"
     gene            complement(2941..3345)
                     /locus_tag="VFU45_09110"
     CDS             complement(2941..3345)
                     /locus_tag="VFU45_09110"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5359266.1"
                     /translation="MSTKAEPLQGEALMASLTVKDLEKSAAWYTGVVGFTEVRRTERD
                     GKLRSIAVQAGNVRINLNQDDGARGWERVKGEGFSFTVSVTQSVDAVAAHIKAHGGTL
                     DTEPADMPWGVRAFRIRDPDGYKWSVSRPLNA"
     gene            complement(3372..>3638)
                     /locus_tag="VFU45_09115"
     CDS             complement(3372..>3638)
                     /locus_tag="VFU45_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359267.1"
                     /translation="LAVKGVVKAKGGYAVTVENVGGMDAPFDLVATLTDGKVVRQHQT
                     AAVWSRDGRTAVVTVPAAGTAASVTIDGGIWVDADTTNNTWHRH"
BASE COUNT          477 a         1254 c         1380 g          527 t
ORIGIN      
        1 ggatcagacc accgccccga agagccggcc gacgagtgcg gtgcaggcca tcgccacggc
       61 gccccagaac gcgacccgga cggcgccgcg ggtgagcgac gcgcccccga ggcgggccgc
      121 gacggcgccc agaacgagga ggagcgccag tgtaaggacg acgatggccg ccgaaagccc
      181 acggatggcc cagaacgcgg cgaggaacgc ggggggcgcg gcccccacgg caaaggacgc
      241 ggcggacgcc acggccgcct ggatcgggcg ggcgcgggtg atctcgtgga tgccgagctc
      301 gtcgcgggcg tgggcgccga gggcgtcgtg ggccatcagc tggtcggcga cgcggatggc
      361 cagttcccgg tcgaggccac gctttacgta gatatcggcc agctcccggc gctcggcctc
      421 gggcgcggcg gcgagttcgc ggcgctcccg cgccaggtcg gcgtgctcgg tgtcggcctg
      481 ggagctcacc gacacgtact cgccggcggc catcgagagt gcgcccgccg cgagccctgc
      541 cactgcggcc agcagcacgg cggaacggct cgggtcggcc gcggcgacgc ccacgaccaa
      601 gctgctggtg gagatcaggc cgtcgttggc gccgagcacc gcggcgcgga gccagccgat
      661 ccggtcgccc cggtgcgatt caccgtgcat ggcgcgcgag cccctgcccg ccgacgggag
      721 ccgtctccac ccggtggctg gacccggcga tgagcgggcg ccccttcgcg atcgccgcct
      781 tgacgctcgg cagcgacagc gacgcctggt ggctcgccgc actctcccag acctcggtga
      841 cccagagggc gtcatgatcg gcggtgtcgc gggcgatcac gtagctcagg caccccggca
      901 tcgcgccgga gccttcgagg aggatggctg cgagggcgtc gcgctggccc ggcgtggcgg
      961 tgagcttggc gatgaggccg tacatggtgg tggccggtcg gtgaagtggt gagtggcggg
     1021 tgacgcgtgt ccccacgtcc gggtccccga tgtcccgtgc ggccgacccg ggcggcccgt
     1081 ccgtgggggc gaggccgccc gccgctgccg gggttgcgcg gtcaacgcga catggctgcc
     1141 gacggggccc gccaacgctt gagccgcggc agcacatcga gcagcagcgc gcgggcctgc
     1201 ggggccggca tcacgccggc ctggtccatc gcgtcgacgc tccggtcgag catgtcctgc
     1261 atcgagatgc cggggtcggt gaacgcgccg ttcgcgtagc agtgggtgca gtagtcgttg
     1321 atgcggaagc cggcctggtc ggtgccgaag tcctccggcc ggttcagggg catcgaacag
     1381 ctctggcaga acggtccctt ggtctcgtac gtgggcatat gatcctcgct ggaacggtgg
     1441 cgcggtcatc tgatgacacg tggaccacca gcgtacggcc cggggcagta agccggggtg
     1501 aacccggagt gaaatggtcg ttcgacggtc agctcgggcg atgacgcggg tgcctggccg
     1561 cccgccgggt caggccgccg gcctcgagcc actggtagaa ctgctcgatc cagtggtcgc
     1621 tcgtggtgcc ctggtggcgg aggccgaagc cgtggccgcc gctgccatag atgtgggcct
     1681 cgggccggtc gcccgctgcc atgagcgcgg cgtagaaccg ggcgacggca tcgccggcca
     1741 ggcggtcatc ctgcgcccac gccaggaacg tgggcggcag gtgcgggggg atggcgggca
     1801 tggcgccgag gggccccccg tagatcatcg cggcaaagtc ggggcgtgcg gcggaatcgg
     1861 gctggaggag cgcgccgctg gcgaccatac cgccggcgga gaagccgagg atcccgatgc
     1921 ggtcgggcaa cactccccac tcggcggcat gcgcgcggac caccttcagg gcctggatgc
     1981 cgtcggcgat cccgtagcga ccggcggtgt caaagtccat ttccgggatc ccgtcctgct
     2041 tcttctcgac cgtccggtac ttgagcacga aggcggcgat cccgcgacgc tggagccagc
     2101 gtgcggcgtc ggtgccctcg aggtccagcg cgagcgccac gaaggcgcca cccggcgcga
     2161 tgatgacgcc ggtgccggtc gccgtgctgc gggcggggag gtaggcggtg agcgtcgggg
     2221 tcacgacgtc gatcaccacg gtgcccaggg gcgtgccggt gtaggtcttc tcctgctggg
     2281 tccagtgttc agatccgggc gcggtgccgg gccagacgcg gacgacctgc gcgcgggcgc
     2341 cggtcgccgc ggccacgatc gccagcgcgg tcagccatgg tgctcgatgc agggtgcctc
     2401 cggcgggccg tcgcccgtca gcgcaggtcg tggaggtaat tgtgcatgtg ctcgagggtc
     2461 gcctgctgct cccggacctg gtgcgccagg aggtcgccga tggtcacgat ccccttgagc
     2521 gccccgccct cctcgaccgg gaggtgccgg acgcgccggg cggtcatcag cgcggcgcac
     2581 tcctcgacgt cgagatcggg gggacaggtg atgacgtcgg cggtcatcac ggcggagacc
     2641 ctcgtggtgg cgggatcgag cccccgcgcc acgacccggc gcaggacgtc gcgctcggtg
     2701 aagatgccgg cgagcttccc gccgtccagc accgccacgc cgccgacgtt gcgggtgttc
     2761 atcagctcgg cgacgtggag taccgtgtcg gtcggctgga ccgagatgac ctcgctccct
     2821 ttccgggtca gcaggtcgcg gagggtggcc atggtgcgct cctcgtggtg ggagctctag
     2881 cttacggccc ggtcccgccc ggcgccatag cggcaccggg cgggccgcgc cggtcgcccg
     2941 tcaggcgttg agcggcctcg atacggacca cttgtagccg tcggggtcgc ggatgcggaa
     3001 cgcccggacg ccccatggca tgtcggcggg ctcggtgtcg agggtgccgc cgtgcgcctt
     3061 gatgtgcgcg gccacggcgt ccaccgactg ggtgacggac acggtgaacg agaatccctc
     3121 gcccttcacg cgctcccagc cgcgggcccc gtcatcctgg ttcaggttga tccggacgtt
     3181 gccggcctgc acggcgatgc tccggagctt gccatcgcgc tcggtgcggc gcacctcggt
     3241 gaagccgacg acgccggtgt accacgcggc gctcttctcg aggtccttca cggtgaggga
     3301 ggccatcagc gcctccccct gaagtggttc tgccttggtg ctcatcggtc tccagatggt
     3361 ggaagtggtc gctagtgccg gtgccaggtg ttgttcgtcg tgtctgcgtc gacccagatc
     3421 ccgccgtcga tcgtcaccga cgctgccgtc ccggccgcgg gcaccgtcac gacggccgtc
     3481 cgcccgtcgc gcgaccacac cgccgcggtc tggtgctggc gcacgacctt cccgtcggtg
     3541 agggtcgcca cgaggtcgaa gggcgcgtcc atcccgccga cgttctcgac ggtcacggcg
     3601 tagccgccct tggccttgac cacacccttc accgccag
//
LOCUS       DASUAP010000335         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1101842, whole genome shotgun sequence.
ACCESSION   DASUAP010000335 DASUAP010000000
VERSION     DASUAP010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101842"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="VFU45_09120"
     CDS             <1..1535
                     /locus_tag="VFU45_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU5359268.1"
                     /translation="RRTGPRKIALEYGFPPATKWDPAIPGVEIGPRADRFVRSSADAG
                     PLPADDEDIAFAPVTHLSRWIEKRQLTAERLTRIYLARIQKFDPKLRCIITLNADGAL
                     AAAQRADREIAAGKYRGPLLGIPYGVKDLLDTAGIRTTWGAEPFRDRVPTADSAVVSR
                     LNAAGAVLLAKLSLGALALNDIWFGGQTMNPWVLEEGASGSSAGPGASTAAGLVGFSI
                     GSETGGSIIAPTMRCGINGLRPTYGRVPRTGAMTLCWSLDKLGPMTRSVEDTMLVLQA
                     ITGPDAGDVASVPCKFDFDAGASVKGLKVGYFPAWMKEAPATDVDRASLDQLRKLGMI
                     PTEVSLPDWPYDSLNLLLFAEGAAAFEELVLSGQVDQLRAQVPDAWPNLFRQARFLSA
                     VDFVQADRLRRKVAQEMARVMAQVDLLVVPSFRDEMLVISNNTGHPSLTMRTGFVKIS
                     EARSDWAPDPANPLPKFDPPRRVPHGITFIGRLFDEGTLGRVGVALERELGVMGERPA
                     GF"
     gene            complement(1637..>2025)
                     /gene="dps"
                     /locus_tag="VFU45_09125"
                     /gene_synonym="pexB"
     CDS             complement(1637..>2025)
                     /gene="dps"
                     /locus_tag="VFU45_09125"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEU5359269.1"
                     /translation="QSQCKQAHWNVKGPSFIALHKLFDEINEGVAGYVDLLAERVVQL
                     GGVAEGTVGVVAERSTLLDYPIGLSKGTEHVAALSDALARFGSSLRTGIDEMADLKDP
                     ASADILTEISRGVDQWLWFVEAHLGA"
BASE COUNT          293 a          716 c          710 g          306 t
ORIGIN      
        1 agcggcggac cgggccgcgc aagatcgcgc tcgaatacgg cttccccccg gccaccaagt
       61 gggaccccgc catccccggc gtcgagatcg gcccccgggc ggatcgcttc gtccggagca
      121 gcgccgatgc ggggccgctc ccggccgatg acgaggacat cgccttcgca cccgtcaccc
      181 acctctcccg ctggatcgag aagcgccagc tcaccgccga gcgcctgacg cggatctacc
      241 tggcgcggat ccagaagttc gatcccaagc tccgctgcat catcaccctc aacgccgatg
      301 gggcgctcgc cgccgcgcag cgtgccgacc gggagatcgc agccggcaaa tatcgcggcc
      361 ccctcctcgg catcccatac ggggtcaagg atctcctcga caccgcgggg atcaggacca
      421 cctggggagc ggagcccttc cgcgatcgcg tccccactgc cgactccgcg gtcgtgtccc
      481 ggctcaacgc cgccggtgcc gtgctcctgg ccaagctgag tctcggcgcc ctggcgctca
      541 acgacatctg gttcggtggg cagacgatga acccctgggt cctggaggag ggggcgtcgg
      601 ggtcaagcgc cggcccgggg gcgtcgaccg cggcggggct cgtcggcttc tcgatcggga
      661 gcgagaccgg cgggagcatc atcgcgccca cgatgcgctg cgggatcaac ggcctccgcc
      721 cgacctatgg gcgggtgccg aggactgggg cgatgacgtt gtgctggtcg ctcgacaagc
      781 tcgggccgat gacgcggagc gtggaagaca ccatgctcgt gctgcaggcc atcaccgggc
      841 ccgacgcggg tgacgtggcg agcgtcccct gcaagttcga cttcgatgcc ggcgcgtcgg
      901 tcaaggggct caaggtcggc tacttccccg cgtggatgaa ggaagcgccc gccaccgatg
      961 tcgaccgtgc gtcgctcgac cagctccgga agctcggcat gatcccgacc gaggtgtcgc
     1021 tccccgactg gccgtacgac tcgctcaacc tgctgctctt cgccgagggg gccgccgcgt
     1081 tcgaggagct cgtgctgagc gggcaggtcg accagctgcg ggcgcaggtg cctgatgcct
     1141 ggcccaatct cttccgccag gcgcgcttcc tctcggccgt ggacttcgtg caggccgacc
     1201 ggctccggcg gaaggtggcg caggagatgg cgcgggtgat ggcgcaggtc gacctgctgg
     1261 tggtgccgtc cttccgggac gagatgctcg tcatctccaa caacaccggg catccgtcgc
     1321 tcacgatgcg gaccgggttc gtcaagatct cggaggcccg gagcgactgg gcgcccgacc
     1381 cggccaatcc gctgcccaag ttcgacccgc cgcggcgggt gccgcacggg atcaccttca
     1441 tcgggcggct ctttgacgag gggacgctcg ggcgggtggg agtggcgctg gagcgggagc
     1501 tcggcgtgat gggagagcgg ccggcggggt tctagggggg gacggatctc cccctctcca
     1561 cgaacgtgta gagggggcag gggggtgagg ctttcgtgag aggggccggg cgtgaggctg
     1621 cgagaaggtc ctgcgctcac gcccccaggt gcgcctccac gaaccagagc cactggtcga
     1681 cgccgcggga gatctccgtc aggatgtcgg cgctggccgg gtccttcagg tccgccatct
     1741 cgtcgatccc cgtccgcagc gaactcccga agcgcgccag tgcgtcggag agcgccgcga
     1801 cgtgctccgt ccccttgctg agcccgatcg ggtagtcgag cagcgtggag cgctcggcca
     1861 ccacgcccac cgtgccctcc gcgacgccgc ccagttgcac cacccgctcc gccagcaggt
     1921 cgacgtagcc cgcgaccccc tcgttgatct cgtcgaagag cttgtgcagc gcgatgaacg
     1981 acggcccctt cacgttccag tgcgcctgct tgcactggct ctgga
//
LOCUS       DASUAP010000336         5141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1020157, whole genome shotgun sequence.
ACCESSION   DASUAP010000336 DASUAP010000000
VERSION     DASUAP010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020157"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFU45_09130"
     CDS             complement(<1..657)
                     /locus_tag="VFU45_09130"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU5359270.1"
                     /translation="MALPVAAGARRATVQLTRMARAGSTQDLVHQLAEEGAPHGTAIV
                     AEEQVQARGTRGRTWHAPRGGVWMSVLCRPAVPAAAEVLSLRVGLAVAAALEAQAQVS
                     LRLKWPNDLMLGERKLGGILCEARWRGDRLAWVTVGIGINVTNPIPPDVASTAARLAD
                     HDPRAAADRLAESVAEAVAGIDGSAAMLTTQELATFAERDWLRGRAIVAPEPGVAAGI
                     D"
     gene            complement(642..1451)
                     /locus_tag="VFU45_09135"
     CDS             complement(642..1451)
                     /locus_tag="VFU45_09135"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU5359271.1"
                     /translation="MTWMTALPLAVKAAILGVVEGITEFLPVSSTGHLIVAGDWLGWT
                     GDDAKTFEIFIQLGAILAVVWIYRARLLAEVRGIGRDAASNVFAASLLIAFLPAAVVG
                     LLLHHWITAHLFSPQVVAWALLIGGILILVIERFHRVPTVTDATMVPPRPALGTGLAQ
                     VLSLIPGTSRSAATILGGYSCGLTRTAATEFSFFLAIPVMFAATLYELWKGRHGLGGV
                     DVVPFVVGFLTAFVSAVIVVKAFLRSVSGHSFTVFAWYRIAFAAFLLWLYR"
     gene            complement(1448..2572)
                     /gene="bshA"
                     /locus_tag="VFU45_09140"
     CDS             complement(1448..2572)
                     /gene="bshA"
                     /locus_tag="VFU45_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEU5359272.1"
                     /translation="MRIGITCYPTYGGSGALATELGIELARRGHEVHFITEAAPFRLR
                     GFHERVTYHEVETRIGNYPLFTHFPYTLALASKQHEVALRERLDILHVHYAIPHATTA
                     WIAREMLGAVHPLKVVTTLHGTDITLVGQEAIFFEITKFSIERSDRVTAVSADLRDET
                     YRAFGCGSSDIRVIPNFVNLAEYRPGDGHARAELAPAGHRIVTHISNFREVKRVKDVV
                     RVFARIRKAMPATLLMVGDGPEREDAEAEARALEVAKDVRFFGRLDTVAEILQASDLF
                     ILPSQSESFGLAALEAMACGVPVVASRAGGIPEVVEDGVNGILEPPGSVEAMGRRAVD
                     LLRAPDRLAAMRAAAAARAAHFSADAVVPQYEALYREVLA"
     gene            complement(2681..3613)
                     /gene="miaA"
                     /locus_tag="VFU45_09145"
     CDS             complement(2681..3613)
                     /gene="miaA"
                     /locus_tag="VFU45_09145"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU5359273.1"
                     /translation="MAAAKLRIPVLVGPTAVGKTATALALSAHWPLEIVSADSRQVYR
                     RLDIGTAKPTRKERSRVPHHGLDLIDPGHRYSAGRFARDATGWIAEIAARGKLPVVVG
                     GTGFYIRALAEGLFHEPPLDPERRRALEAWLLGHDALELVRWAARLDPGFKGGGRQRA
                     TRTIEVALLTGRPLTFWQGAAKQHGLIEPWYVVLTAPRPVLHQRIAARAEEMVRRGLV
                     EEVASVLADGAPAHGPGLDGVGLREAVEYLHGERSRESVAGAITIATRQYAKRQETWF
                     RHQLPGPVLALDSVRPPEELAAEIAGAWAAAGPP"
     gene            complement(3600..>5141)
                     /gene="mutL"
                     /locus_tag="VFU45_09150"
     CDS             complement(3600..>5141)
                     /gene="mutL"
                     /locus_tag="VFU45_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU5359274.1"
                     /translation="GMGREDALLALDRHATSKIRSAGDLVGVGSFGFRGEALPAIASV
                     SRLALTTAEAGGTGTEVAVTGGRIEQVSDVARQPGTTVAVRALFFNTPARRKFLRSVS
                     SESRVSFDAVAILALAHPAVGFELVADERTRLVLPAGQPLVERLAAVWGADLAGTLVP
                     VSYAAGAVRVEGFIQRPGDARPTGRRTQLLVNGRPFRDNFLIRAAEAGYRSAIHPGDR
                     PSVFLHLTVPVAEVDVNVHPAKLEVRFLNRVAVERAVEEGVRHALGELIAAVPVGEWR
                     PIPAPLRPSGSGEVWAGTEELPAPAAPDVPEPLPLLPDTGPAAREDAPFGGTLVQFFD
                     TYIAYEAPDGIVIVDQHSAHERVLYEQVMADLTRGGVAAQRLLLPFTVDLTDEELDVV
                     ETHRDALAQVGFEVEAFGGRTVAVHGVPAPHRRFDALACFREVVADLARGRFGGWANR
                     LERFVATYACRAAVKAGQRLNEREMRELLVRLMATMLPPHDVHGRSTIVQLPREELER
                     RFGRR"
BASE COUNT          738 a         1865 c         1887 g          651 t
ORIGIN      
        1 gtcgatcccg gcggccacgc ccggctccgg cgccacgatg gcacggccac ggagccagtc
       61 ccgctcggca aaggtggcca gctcctgcgt ggtgagcatc gcggcgctcc cgtcgatgcc
      121 ggcgaccgcc tccgccaccg actcggccag ccggtccgcg gcggcccggg gatcatggtc
      181 ggccagccgc gccgcggtgc tcgcgacgtc gggcgggatc gggttggtca cgttgatgcc
      241 gatcccgacg gtgacccacg ccagccggtc gccgcgccag cgcgcctcgc agaggatgcc
      301 gcccagcttc cgctcgccca gcatcaggtc gttgggccac ttgagccgga ggctgacctg
      361 cgcctgcgcc tcgagtgccg cggccaccgc gagccccacc cggaggctca gcacctccgc
      421 ggcggcgggg accgccgggc ggcagaggac gctcatccag accccgccgc ggggtgcgtg
      481 ccaggtgcgg ccccgggtgc cgcgcgcctg cacctgctcc tcggccacga tcgccgtgcc
      541 gtgcggcgcg ccctcctcgg ccagctggtg caccaggtcc tgcgtcgagc cggcccgcgc
      601 catccgcgtg agctgcaccg tggcccgccg tgcgcccgcg gctaccggta gagccacagc
      661 aggaacgccg cgaacgcgat ccggtaccac gcgaagaccg tgaagctgtg gccggacacg
      721 gaccgaagga acgccttgac cacgatgacg gcggacacga aggccgtgag gaacccgacg
      781 acgaaaggca ccacgtccac tccgccgagt ccgtggcggc ccttccagag ctcgtagagc
      841 gtcgcggcga acatcaccgg gatagccagg aagaacgaga actcggtcgc ggcggtgcgg
      901 gtgagcccgc acgaatatcc gccgaggatg gtggcggcgg agcgcgacgt gccggggatg
      961 agcgagagca cctgcgccag gccggtgccg agcgcagggc gcgggggcac catcgtggcg
     1021 tcggtgaccg tgggcacgcg gtggaaccgc tcgatgacga ggatcaggat gccgccgatg
     1081 aggagcgccc acgccaccac ctgcggggaa aagaggtgcg cggtgatcca gtggtggagg
     1141 aggagcccca ccacggcggc ggggaggaac gcgatcagga gcgaggcggc aaagacattg
     1201 ctcgccgcgt cgcgcccgat cccccgcacc tccgccaaca ggcgggcacg gtagatccag
     1261 accacggcga ggatggcacc aagctggatg aagatctcga acgtcttggc gtcatcgccg
     1321 gtccagccaa gccagtcgcc ggcgacgatc aggtggccgg tggaggagac cggcaggaac
     1381 tcggtgatcc cctcgaccac gccgaggatc gccgccttga ccgcgagcgg gagcgccgtc
     1441 atccacgtca tgccaggacc tcgcggtaga gcgcttcata ctgggggacc acggcatccg
     1501 cggagaagtg cgcggcccgc gcggccgcgg cggcgcgcat ggcggccagg cggtcggggg
     1561 cgcggagcag gtccacggcg cggcggccca tcgcctcgac cgatccgggg ggttcgagga
     1621 tgccgttcac gccatcctcc accacctccg ggatcccgcc cgcccggctg gccaccaccg
     1681 gcacgccgca ggccatcgcc tcgagggccg cgagcccgaa ggactcgctc tggctcggca
     1741 ggatgaagag gtccgatgcc tggaggatct ccgccacggt gtcgagccgg ccgaagaagc
     1801 ggacgtcctt cgccacctcg agcgcgcgcg cctccgcctc ggcgtcctcc cgctcgggcc
     1861 cgtcgcccac catcaggagg gtggccggca tggccttccg gatccgggcg aagacccgca
     1921 ccacgtcctt cacccgcttc acctcccgga agttggagat gtgggtgacg atgcggtgcc
     1981 ccgccggtgc cagctcggcg cgcgcgtgcc cgtcgcccgg ccggtactcc gccaggttca
     2041 cgaagttggg gatgacccgg atgtcgctgc tgccgcatcc gaaggcgcgg tacgtctcgt
     2101 cgcggaggtc ggcggagacc gcggtgacgc gatcggaccg ctcgatcgag aacttggtga
     2161 tctcgaagaa gatcgcctcc tggcccacca gcgtgatgtc ggtgccgtgg agcgttgtca
     2221 cgaccttgag gggatggacg gcgccgagca tctcgcgcgc gatccacgcg gtggtggcgt
     2281 gggggatggc gtagtgcacg tggaggatgt cgagccgctc gcggagcgcc acctcgtgct
     2341 gtttcgatgc gagggccagc gtgtacggga agtgggtgaa gagggggtag ttgccgatcc
     2401 gggtctcgac ctcgtggtac gtgacccgct catggaaccc gcgcaggcgg aagggcgccg
     2461 cctcggtgat gaagtggacc tcgtgccccc gccgggcgag ctcgatgccg agctcggtgg
     2521 ccagggcgcc ggagccgccg taggtggggt agcaggtgat gccgatgcgc attgaggaag
     2581 tgtaatgggt cgtgggtcgt tcgtcgttgg tcgtgggtcg tgggtcgtgg gtcgcgggtc
     2641 gagggtcgcg ggtcgcggga aacggggcga tctcaggccg tcacggtggg ccggccgccg
     2701 cccacgcccc cgcgatctcc gccgccagct cctcgggcgg gcgcacgctg tcgagcgcca
     2761 gcaccggccc cgggagctgg tgccggaacc aggtctcctg ccgcttggcg tactggcggg
     2821 tggcgatggt gatggcgccg gccaccgatt cccgggagcg ctcgccgtgg aggtattcca
     2881 ccgcctcgcg gagccccacg ccgtcgagcc cggggccgtg cgcgggtgcg ccatcggcga
     2941 gcaccgaggc cacctcctcc accagcccgc ggcgcaccat ctcctccgcc cgcgcggcga
     3001 tccgctggtg caggacgggg cgcggcgccg tgagcaccac gtaccacggc tcgatgaggc
     3061 cgtgctgctt ggcggccccc tgccagaagg tgagcggccg gccggtgagg agcgccacct
     3121 cgatggtccg cgtggcgcgt tgccggccgc cacccttgaa gcccggatcg agccgcgccg
     3181 cccaccgcac cagctcgagc gcatcgtgcc ccaagagcca cgcttccagc gcccgccggc
     3241 gctccgggtc gagcggcggc tcgtggaaga gcccctcggc gagcgcccgg atgtagaacc
     3301 ccgtccctcc caccaccacc ggcaacttgc cgcgcgccgc gatctcggcg atccaccccg
     3361 tcgcgtcgcg cgcgaaccgg ccggcgctgt agcggtgccc cgggtcgatc aggtcgaggc
     3421 cgtggtgcgg cacccgcgag cgctccttcc gcgtgggctt ggcggtgccg atgtcgagcc
     3481 gccggtatac ctggcgggaa tcggcggaga cgatctccag cggccagtgc gcggagaggg
     3541 ccagcgccgt cgccgtcttg cccacggcgg tcgggccgac gaggaccggg atgcggagct
     3601 tagcggcggc caaaccggcg ctccagctcc tcgcggggga gttgcacgat ggtcgaccgg
     3661 ccatgcacgt cgtggggcgg cagcatcgtg gccatcaggc ggaccaggag ctcccgcatc
     3721 tcccgttcgt tgagccgctg tcccgccttg accgccgcgc ggcaggcgta ggtcgcgacg
     3781 aaccgctcca gccggttggc ccacccgccg aagcggcccc gcgccaggtc agccacgacc
     3841 tcgcggaagc acgccagcgc gtcgaagcgc cggtgcggcg cgggcacccc gtgcactgcg
     3901 acggtgcgcc cgccgaacgc ctccacctcg aacccgacct gcgccagcgc atcgcgatgg
     3961 gtctcgacga cgtcgagctc ctcgtcggtc aggtcgacgg tgaacggcag gaggagccgc
     4021 tgggcggcga ccccaccgcg cgtgaggtcg gccatcacct gctcgtagag cacccgctcg
     4081 tgggcggaat gctggtcgac gatgacgatc ccgtcgggcg cctcgtaggc gatgtacgtg
     4141 tcgaagaact ggaccagcgt cccgccgaag ggcgcatcct cccgcgcggc gggcccggtg
     4201 tcgggcagga gcggcagcgg ttccggcaca tccggcgccg ccggcgccgg gagctcctcc
     4261 gtgccggccc acacctcccc gctccccgac gggcggagcg gcgccgggat cggccgccat
     4321 tctcccaccg gcactgcggc gatcagttcg cccagggcgt gccgcacccc ttcctcgacc
     4381 gcgcgttcga ccgccacgcg attgaggaac cggacttcga gcttggcggg atggacgttg
     4441 acgtcgacct cggccaccgg cacggtgagg tgcaggaaga ccgacggccg gtcgccggga
     4501 tggatggccg agcggtaccc cgcctcggcc gcgcggatga ggaaattgtc gcggaacggc
     4561 cggccgttga cgagcagctg ggtgcgccgg ccggtgggcc gcgcatcgcc gggccgctgg
     4621 atgaatccct cgacccgcac cgcacccgcc gcgtacgaca ccggcacgag cgtgccggca
     4681 aggtcggcgc cccacaccgc tgcgagtcgc tcgaccagcg gctggccggc aggaaggacg
     4741 agccgggtgc gctcatcggc caccagctcg aacccgaccg ccgggtgtgc cagcgccagg
     4801 atcgcgaccg cgtcgaacga cacccgcgac tcgctcgaga ccgagcggag gaacttccgg
     4861 cgggcggggg tgttgaagaa cagcgcccgc accgcgaccg tggtccccgg ctgccgggcg
     4921 acatcgctga cctgctcgat ccgcccgccg gtgaccgcca cttcggtccc cgtgcctccc
     4981 gcctccgcgg tggtgagcgc caggcgcgac accgatgcga tcgcggggag cgcttcgccg
     5041 cggaaaccga aggacccgac gcccacgagg tcgccggcac tccggatctt gctcgtggcg
     5101 tggcggtcga gcgcgaggag cgcgtcctcc cgccccatcc c
//
LOCUS       DASUAP010000337         5688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_856633, whole genome shotgun sequence.
ACCESSION   DASUAP010000337 DASUAP010000000
VERSION     DASUAP010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856633"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="VFU45_09155"
     CDS             <1..1580
                     /locus_tag="VFU45_09155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5359275.1"
                     /translation="GLKMPDVGAFQQQLLADAWRLGRRAPVTALTDAVRPDQQMAVRY
                     PATYSLARLHPAAAANALLTLAADQSSAIRGLAIRAMTRAFADSAHLPRADVARAAVP
                     GLGDPDPGIRIDALRSLGSFTGDSTLSGDVIPLTDDGVGNVEVQALATLGALGGTRAA
                     ALLARVAADKRPWAVRRQALLSLARVDGAAFRTAVGPWTRSGDWRDRAVAAEGWASLK
                     GSAYPVDLLGDPDGRVVAAALQAWSGGAAGNDTALVNAARRHLADADAAVRSVAADII
                     SRTPVAADLAPLTAAWTRAQRDSFPEAGESALAAILALSKTPEGAAAARFAATAATPP
                     AALGYLYRAWAEANWPELSDHWGPSTPIATGRSLEDYRSIARQYVVGPSDQRSPQVTV
                     EVDGKGSMVLTLFGADAPLTVANFLRLVDAGYFNGDRWHRVVPNFVVQDGDPRGDGWG
                     GPGTVIRDEINRHRYRTGTVGMALSGPDTGGSQWFITLGPEPHLDGNYTAFGEVSSGN
                     VVLGRILQGDLIRSIHR"
     gene            1577..4384
                     /locus_tag="VFU45_09160"
     CDS             1577..4384
                     /locus_tag="VFU45_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359276.1"
                     /translation="MTRRRAVLLALLLAGGVRPVRAQLTEFGQNKVQYRTLDWHVMHG
                     PHVDLYFYPAEAALAPTALAWAEESYDTLARKFDHEVAARIPLLVYATHADFEQTNVL
                     PFSPPEGVLGVTDYLKRRVTLPFRGNYAEFRGTLRHEMTHVFQLDLEGDAYARTFHDA
                     SSFLPLWWSEGLAEYFSAGQDARDEMIMRDLTISGRLPSLHDLGYVEGAIVYPIGGRI
                     HQWLGRTYGDWRIAKFYHDLWRYDDFDDAIYGTYGRTLAQLNAEFQEAMRRAYYPAIA
                     ERRALDASAVRIAAGAVKPVLTADGDTAVYFSAPRGYVVAEARSLDGGPARTLVTAGR
                     SADIESFHPFESRIDAGRPGYLLFSTRHEDRDALVVWDIARQQVVGRYQFPGLVSIVS
                     PAWLPGDSAVVFSGLASSGVSDLYRFTFAGERLEHLTDDRYQDLDPSPSPDGTRIVFA
                     SDRTAGGLDGAVNLFVLDLATGSVRQLTAGPWVDEAPHWAANGRIYFASSRDSVLNTF
                     SVDSAGNGRRETSAWTGTYDPTWVPGRDAILVGGFHDLTFGVYLAPPDTMARRDTFSL
                     AAAAPAGRWTWPAPAGATAEVTAARPYRSRYTLDFALAEAEFIPGVATVQGATALFSD
                     LLGDHVVIANFATYQGPTLGSFFDNINASALYLDQSRRLNWGVGAFRAKGDVYESGYT
                     VDYTEKATGAFGFVRYPLSPFSRIDGTFVLAHSDRFDFTLPVSDLHRVGLIASQYLSY
                     VFDNSAWLPTGPIDGTRFAVTGGISSDLTNSRFDNWVLGADLRQYFRLSRQSAYAVRA
                     FGFESGGDRPRRINIGGTLGLRGYPNYGYIVGTHAVMLNQELRFPVFDFLGLGFPVQE
                     IRLPGVQGAIFADVGKAWYDQTTATSTIGSYGLSFRMALTPVAVLRLDWGDRWTTGNY
                     DGYGLGVVGRRFVSFFFGYNY"
     gene            4486..5082
                     /locus_tag="VFU45_09165"
     CDS             4486..5082
                     /locus_tag="VFU45_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359277.1"
                     /translation="MTPLPIDSVATWTRATLPAGRWLHRFKWQYQDDRSAAGGRGSAH
                     IAGPDSIRFDVAGPLGASPAAALVVGDSARWVRPEDAIHKLVPSYPLMWAVFGVARIP
                     EGATLALAGRAGDAEVWRAIAGADTIDWARSAGRLVAEVRRDGQVVGRADTRFGPDGR
                     LRSARLTMPAVPARLDLTFTLSDTPARFPADVWRPREP"
     gene            5144..5551
                     /gene="lptE"
                     /locus_tag="VFU45_09170"
     CDS             5144..5551
                     /gene="lptE"
                     /locus_tag="VFU45_09170"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEU5359278.1"
                     /translation="MRTVAVLPFDNLTSDPTLTQDVNIAVRNAMEQNLGLRQAGVDQA
                     DALVRGTITRYEPDVPVAYTGTAQNQVAVTKRLVQITVSIEILDQKQNKTLWQRSGLT
                     VEGDYDPGREQDGRKKALDQLVNNLVEGAQSQW"
BASE COUNT          778 a         2106 c         1897 g          907 t
ORIGIN      
        1 ctggcctcaa gatgcccgat gtcggggcgt tccagcagca actgcttgcc gatgcgtggc
       61 ggctgggccg ccgggcaccg gtcacggcgc tcacggacgc ggtccgaccc gaccagcaga
      121 tggcggtgcg ctatccggcg acctactcgc tcgcccgtct ccatccggcg gccgccgcca
      181 acgcgctcct caccctcgcc gccgatcaat cttccgccat ccgcggcctc gccatcaggg
      241 ccatgacccg ggcgttcgcc gacagcgctc atctgccgcg cgccgacgtg gcgcgtgccg
      301 cagtccctgg attgggagac ccggaccccg gcatccggat cgatgcattg cggtcgctgg
      361 gctcgttcac cggcgattcg accctgagcg gggacgtcat ccccctcacc gatgacgggg
      421 tcgggaacgt cgaggtgcag gcgctcgcga ccctcggcgc gctcggcggt acccgcgccg
      481 cggcgctcct ggcgcgtgtg gccgccgaca agcgcccctg ggccgtccgg cggcaggcgc
      541 tcctctcgct cgcgcgggtc gatggggcag cctttcgaac cgccgtcgga ccttggactc
      601 gctccggcga ctggcgggat cgcgccgtcg ctgccgaagg atgggcttcc ctcaaggggt
      661 ccgcgtaccc ggtcgacctg ctcggcgatc ccgacgggcg ggtggtggcc gccgcgttgc
      721 aggcatggtc gggcggcgcc gcggggaatg atacggcgct cgtgaatgcg gcccggcgcc
      781 acctggccga tgccgacgcc gccgtccgca gcgtagccgc cgacatcatt tcccgcacac
      841 ccgtcgcggc cgatcttgcc ccgctcactg cggcctggac ccgtgcccag cgggattcct
      901 ttcccgaggc gggggagtcg gccctggccg ccatcctggc gctctcgaag acacccgaag
      961 gggccgccgc cgcgcggttc gccgcgaccg ccgcgacgcc gcccgcggcc ctgggttacc
     1021 tctaccgcgc gtgggccgag gccaactggc ccgagctcag cgaccactgg gggccctcga
     1081 cgccgatcgc caccggccgc tcgctcgagg actaccggtc gatcgcgcgg cagtacgtcg
     1141 tcggcccctc cgatcagcgg tcgccccagg tgaccgtcga ggtcgacggc aagggttcga
     1201 tggtgctgac gctcttcggc gccgacgcgc cgctcaccgt ggccaacttc ctccgcctcg
     1261 tcgacgccgg ctacttcaat ggcgaccgct ggcaccgggt ggtccccaac ttcgtggtgc
     1321 aggatggcga cccgcgcggc gacggctggg gcggcccggg cacggtcatc cgtgacgaga
     1381 tcaaccgcca tcgctatcgc accgggacgg tggggatggc gctctccggg cccgacaccg
     1441 gcggcagcca gtggttcatc accctgggcc cggagccgca cctcgacggc aactacaccg
     1501 cgttcggtga agtgtcgtcg ggcaacgtgg tgctgggccg gatcctccag ggtgacctca
     1561 tccggtccat ccatcggtga ctcgccggcg ggcggtcctc ctcgccctcc tgctcgcggg
     1621 aggggtgcga cctgtccggg cgcagctgac cgagttcggc cagaacaagg tgcagtatcg
     1681 caccctcgac tggcacgtga tgcacggccc ccacgtcgac ctctacttct acccggccga
     1741 ggccgcgctg gcgcccaccg cgctcgcctg ggcggaggag agctacgaca cccttgcccg
     1801 gaagttcgac cacgaggtcg ccgcgcggat cccgctcctc gtctacgcca cccacgccga
     1861 cttcgagcag accaacgtcc ttccgttctc cccgccggaa ggggtgctgg gcgtcaccga
     1921 ctacctgaag cgccgggtca ccctgccgtt ccgcggcaac tacgccgagt tccgcggcac
     1981 gctgcgccac gagatgaccc acgtcttcca gctcgacctg gagggcgatg cgtacgcacg
     2041 caccttccac gacgcgagct cgttcctgcc gctctggtgg tcggagggcc tggccgaata
     2101 tttctccgcc gggcaggacg cgcgcgacga gatgatcatg cgggacctca ccatctccgg
     2161 ccggctcccc tcgctgcacg acctcggcta cgtcgagggc gccatcgtct atccgatcgg
     2221 cgggcggatc caccagtggc tcgggcgcac ctacggcgac tggcggatcg cgaagttcta
     2281 ccacgacctt tggcgctacg acgacttcga cgatgcgatc tacggcacct acggccggac
     2341 gctcgcccag ctgaacgcgg aattccagga ggcgatgcgg cgggcgtact accccgccat
     2401 cgccgagcgg cgggcgctcg acgcctccgc ggtgcgcatc gcggcggggg ccgtcaagcc
     2461 ggtgctcacc gccgacggcg acaccgccgt ctatttctcg gcgccccggg gatacgtcgt
     2521 ggccgaggcg cggtccctcg acggcgggcc ggcccggacg ctcgtcactg ccggccgttc
     2581 ggccgatatc gagtcgttcc accccttcga gtcgcgcatc gacgccggcc gccccggcta
     2641 cctgctcttc tccacccgcc atgaggaccg tgatgcgctc gtggtctggg acatcgcgcg
     2701 ccagcaggtc gtcggccgct accagttccc cggcctcgtg tcgatcgtct cccccgcctg
     2761 gctccccggc gacagcgcgg tggtcttcag cggcctcgcc tcgtcgggcg tgtcggacct
     2821 ctaccggttc acctttgccg gcgagcggct ggagcacctc accgacgacc ggtaccagga
     2881 cctcgatccg agtcccagcc cggatggcac ccggatcgtc ttcgcgtccg atcgcaccgc
     2941 gggcgggctc gacggcgcgg tcaacctctt cgtgctcgac ctggcgaccg gttccgtccg
     3001 gcagctcacc gccggcccct gggtggacga agcgccgcac tgggcggcga atggccggat
     3061 ctacttcgca tcctcgcgcg acagcgtgct caacacgttc tccgtcgaca gcgcgggcaa
     3121 cgggcggcgg gagacctccg cctggaccgg cacctatgac ccgacgtggg tgccggggcg
     3181 ggatgccatc ctggtgggcg gattccacga cctgaccttc ggcgtctacc ttgcgccgcc
     3241 ggataccatg gcccggcgcg ataccttctc gctcgcggcc gccgcccccg ccgggcgatg
     3301 gacctggccg gcgcccgccg gcgccacggc ggaggtcacg gccgcgcgac cctaccggag
     3361 ccgctatacc ctcgacttcg cgctggccga agcggaattc atccccggcg tggcaacggt
     3421 gcagggggcc acggccctct tcagcgacct cctgggcgat cacgtcgtca tcgccaactt
     3481 cgccacctac caggggccga ccctcggcag cttcttcgac aacatcaacg ccagtgcgct
     3541 ctatctcgac cagtctcggc gcctcaactg gggcgtcggc gccttccggg ccaagggaga
     3601 cgtgtacgag tcggggtaca ccgtcgacta caccgagaag gccaccggcg ccttcggatt
     3661 cgtgcgctat ccgttgagcc cgttcagtcg catcgacggg acattcgtgc tggcgcactc
     3721 cgatcggttc gacttcacgc tcccggtctc ggatctgcac cgtgtggggc tgatcgcatc
     3781 gcaatacctg agctacgtct tcgacaattc ggcgtggctc cccaccgggc ccatcgacgg
     3841 cacgcgcttc gccgtcaccg gcggtatcag cagcgacctc accaacagtc ggttcgacaa
     3901 ctgggtcctc ggcgccgacc tccgccagta cttccggctc tcccgccaga gcgcctacgc
     3961 cgtccgcgcg ttcgggttcg agagcggcgg cgatcggccc cgccggatca acatcggcgg
     4021 cacgctcggg ctccggggct acccgaacta cggatatatc gtcggcaccc acgccgtcat
     4081 gctcaaccag gaactccgct tcccggtctt cgacttcctc gggctcgggt tcccggtgca
     4141 ggagatccgc cttccagggg tgcagggcgc tatcttcgct gacgtcggca aggcgtggta
     4201 cgaccagacc accgccacat ccaccatcgg cagctacggc ctgagcttcc gcatggcgtt
     4261 gacaccggtg gcggtgctcc ggctcgactg gggcgatcgg tggaccacgg gcaactacga
     4321 cggatacggt ctcggagtgg tcggtcgccg gttcgtcagc ttcttcttcg gatataacta
     4381 ctgatgggac aacgagttct gcttgaccgc ccggcagcac ttttctatct tgggttggtc
     4441 tgggcggcat gtgcgccgcg tccggcggcg gtggtcccgg aggcgatgac cccgctgccg
     4501 atcgattcgg tagcgacgtg gacccgggcc acgctgcccg cgggtcgatg gctccatcgg
     4561 ttcaagtggc agtaccagga tgaccggagc gcagcggggg ggcggggcag cgcgcacatc
     4621 gcgggtcccg actcgatccg cttcgatgtc gccggccctc tgggagccag cccggccgct
     4681 gcgctggtcg tcggcgactc cgcgcgctgg gtgcggcccg aggacgcgat ccacaagctg
     4741 gtgccgagct atccgctgat gtgggccgtc ttcggggtgg cacggatccc cgagggggcc
     4801 acgctcgccc tggccggccg ggccggggac gccgaggtct ggcgggcgat cgccggggcg
     4861 gacacgatcg actgggcccg gtcggccggc cggctggtgg cggaggtccg gcgggatggc
     4921 caggtcgtcg ggcgggccga cacccgtttc ggccccgacg gccgcctgcg ctccgcccga
     4981 ttgacgatgc cggcggtccc ggcgcggctg gacctgacct ttacgctgag cgatacccct
     5041 gcgcgctttc ctgcggacgt ctggcgccct cgcgagcctt agcgccgcgc tgtggctctc
     5101 gggttgcctc tacggtttca gtggcggcgg cctgcccaac gacatccgca cggtcgccgt
     5161 actcccgttc gacaacctca cctccgatcc cacgctgacc caggacgtga acatcgccgt
     5221 ccgcaacgcg atggagcaga acctgggcct ccgccaggcg ggcgtcgacc aggccgatgc
     5281 gctcgtccgc ggcaccatca cccggtatga gcccgacgtc cccgtggcct ataccggcac
     5341 ggcccagaac caggtggccg tcaccaagcg cctggtgcag atcacggtgt cgatcgagat
     5401 cctcgaccag aaacagaaca agacgctctg gcaacggagc gggctgaccg tcgaaggcga
     5461 ttacgatccc gggcgcgagc aggatggccg caagaaggcg ctcgaccagc tggtgaacaa
     5521 cctcgtcgag ggagcgcagt cccaatggtg agcggtcggc gggggtcgag ttcctcgggg
     5581 tgcctctttt cgctggtgct cttcggggcg gcgctgtact tcggcatccc gatcgccggg
     5641 gtgtacatcc ggtactacca gctcaccgac gagatccatt cggccgcc
//
LOCUS       DASUAP010000338         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_679530, whole genome shotgun sequence.
ACCESSION   DASUAP010000338 DASUAP010000000
VERSION     DASUAP010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679530"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFU45_09175"
     CDS             complement(<1..199)
                     /locus_tag="VFU45_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359279.1"
                     /translation="MTTHERPFGRYFEDFEPGDIYRHWPGKTVTEYDDHLFCMITMNH
                     HPLHTNAWYAEQEAVHGRNVVV"
     gene            188..943
                     /locus_tag="VFU45_09180"
     CDS             188..943
                     /locus_tag="VFU45_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5359280.1"
                     /translation="MRRHRRERLLACRDPSLFATLRMRAPGRVRIVARVLDLELRNDV
                     YRRPLVTALDRLGLREGWVCADVGAGGGDVTVALARIVGRDGRVYAVDSDPARRDEVA
                     AAAAAAAQAQVVALTQAGEELTLPEPLDLAFCRFVLLHVHSPLAVLSRMKSAVRPGGW
                     VVAQEPITSAGRIGGRPLSMPSARDADVGAVLPGLAVDAGLDLVDAWAEAPAWAGPGP
                     VADYLEGMTEVHPGDEPVILPPLVTVVARRTGR"
     gene            954..2555
                     /locus_tag="VFU45_09185"
     CDS             954..2555
                     /locus_tag="VFU45_09185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5359281.1"
                     /translation="MPSPAPDLVSAAAAIEVARALVERGASHLASLDAASLDDHQAVA
                     YDVAHSAAAAETATCAVEYGRRGPVEAGLAAAFVADAVWDVATRTLGREEAWGIRPGS
                     LDPVMGFLRAHRDPDWLAGLAGESGPRHLDGDFQLVAETFRRFAEEKITPVAEHIHRH
                     NTDIPETIISGLGDLGAFGLSIPEEYGGFATGGEHDYMGMVIATEELSRGSLGAGGSL
                     ITRPEILARALLRGGTEDQKRRWLPALASGEIMAGVAVTEPDFGSDVAGLSVSATRHP
                     DTGDWVISGTKTWSTFAARADVLMVLARTDPDRSAGHRGLSLFVVPKERADGAGFVLR
                     QDGGGRLEGRPIDTIGYRGMHSYELSFDAWRVPADHLIGEDAGLNRGFYYQMEGFENG
                     RLQTAARAVGVMQAAYEAALAYARERVVFGRPVADYQLTRVKLTRMAAIIQACRQYMY
                     AVARMMAKGEGALEASMIKAYVCRAAEWVAREAMQIHGGMGYAEEYAVSRLFVDARVL
                     SIFEGADETLALKVVARRLIDRAKN"
BASE COUNT          356 a          894 c          966 g          396 t
ORIGIN      
        1 cgaccaccac gttccggccg tggactgcct cctgctcggc gtaccaggcg ttcgtgtgca
       61 gggggtggtg gttcatggtg atcatgcaga acaaatggtc gtcgtactcg gtgaccgtct
      121 tgcccggcca gtggcgatag atgtcgcccg gttcgaaatc ctcgaaatac ctgccgaacg
      181 gccgctcgtg cgtcgtcatc gccgtgagag gctactggcc tgccgcgatc cgtcgctctt
      241 cgccaccctg cggatgcgcg cgccgggccg agtgcgtatc gtcgcccggg tgctggacct
      301 ggagctgcgc aacgacgtct accggcggcc gctcgtgacg gcgcttgacc gcctcggtct
      361 tcgggaaggc tgggtctgcg cggacgtcgg cgcgggcgga ggtgacgtga ccgtcgccct
      421 cgcacggatc gtcgggcgcg acgggcgggt ctacgcggtc gactcggacc ccgcccgccg
      481 tgacgaggtg gcggccgcgg ccgccgcggc ggcgcaggcg caggtcgtgg cgctcaccca
      541 ggccggtgag gagctgaccc tccccgaacc gctcgatctg gccttctgcc gtttcgtgct
      601 cctgcacgtg cactcccccc tggccgtgct ttcgcgcatg aagtcggcgg tgaggcccgg
      661 gggttgggtg gtcgcgcagg agcccatcac ctcggccggc cggatcggcg gacgtccgct
      721 gtccatgccc tcggcccgag acgccgacgt gggggcggta ttgccaggtc tcgcggtgga
      781 cgcgggactc gatctggtgg acgcatgggc ggaggccccc gcgtgggcgg gccccgggcc
      841 cgtggcggac tacctggagg ggatgaccga agtacacccc ggcgacgagc cggtgatcct
      901 cccgccgttg gtcaccgtgg tggcgcgccg caccggtcgt tagcctcgac ccgatgccct
      961 ctcccgcacc cgacctcgtc tccgccgctg ccgccatcga ggtcgcgcgc gccctggtcg
     1021 agcgcggcgc gtcgcacctc gcctcgcttg atgcggcctc cctcgacgac caccaggcgg
     1081 tcgcctacga cgtggcccac agtgccgctg cggcggagac cgccacgtgc gcggtcgagt
     1141 acggccggcg cggcccggtt gaagccgggc tggctgccgc gttcgtggcc gacgccgtct
     1201 gggacgtggc gacccggacg ctcgggcgcg aggaggcgtg gggtatccgg cccggcagcc
     1261 tcgacccggt gatgggattc ctccgggccc acagggaccc cgactggctc gcgggcctgg
     1321 cgggtgagag cggcccgaga catctcgacg gcgacttcca gctcgtcgcc gagaccttca
     1381 ggcgcttcgc ggaagagaag atcacgcctg tcgcagagca catccaccgg cacaacacgg
     1441 acatcccgga gacgatcatc tctggactcg gcgacctcgg ggccttcggc ctgtcgatac
     1501 ccgaggagta cggcgggttc gcgaccggcg gcgagcacga ctacatgggc atggtgatcg
     1561 ccacagagga gctgtcgcgc gggtccctgg gtgccggcgg ctccctgatc acccggcccg
     1621 agatcctcgc cagggcgctc ctgcgcggcg ggacggagga ccagaagcgg cggtggcttc
     1681 cggcgctcgc cagcggggag atcatggcgg gcgtggccgt caccgaaccc gacttcggct
     1741 ccgacgtcgc cgggttgagc gtctcagcca cccgccatcc cgacaccggc gactgggtga
     1801 tatcggggac caagacctgg tcgacgttcg cggcgcgcgc ggacgttctc atggtgctgg
     1861 cgcggaccga ccccgaccgc tccgcgggcc accgcgggct ctcactgttc gtcgtcccca
     1921 aagagcgcgc ggacggagcc gggttcgtcc tgcgccagga cgggggcggc aggctcgaag
     1981 gacgcccgat cgacacgatc ggctaccggg ggatgcactc ctacgagctg tcgttcgacg
     2041 cgtggcgtgt gcccgccgac catctgatcg gggaggacgc ggggctcaac cgcggcttct
     2101 actaccagat ggaggggttc gagaacgggc gcctgcagac cgcggcgagg gccgtcggcg
     2161 tgatgcaggc cgcctacgag gccgctctgg cgtacgcgcg cgagcgggtg gttttcggcc
     2221 gacccgtggc cgactaccag ctcacccggg tgaagctcac gcgcatggcc gcgatcatcc
     2281 aggcgtgccg ccagtacatg tacgcggtgg ccaggatgat ggcgaagggc gagggcgcgc
     2341 tggaggcgtc gatgatcaag gcgtacgtgt gccgcgctgc cgaatgggtc gcgcgcgaag
     2401 cgatgcagat ccacgggggg atgggatacg cggaggagta cgcggttagc cggctgttcg
     2461 tggacgcgcg ggtgctctcc atcttcgagg gcgccgacga gacgctcgcg ctgaaggtgg
     2521 tcgcccgccg cctgatcgac cgggcgaaga actagccgct gctgcggtca ggaacaccgc
     2581 ggcatcgctg cgctggcttc aggaacgctt ga
//
LOCUS       DASUAP010000339         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1020278, whole genome shotgun sequence.
ACCESSION   DASUAP010000339 DASUAP010000000
VERSION     DASUAP010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020278"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /gene="gcvH"
                     /locus_tag="VFU45_09190"
     CDS             complement(<1..350)
                     /gene="gcvH"
                     /locus_tag="VFU45_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU5359282.1"
                     /translation="MSNVPADLRYTKDHEYVGKTADAAVVKIGITDYAQGELGDVVFV
                     NLPKPGQQFAAHAAFGTIEAVKAVSELFTPVAGEVVSVNAALDADPAVVNRDPYGAGW
                     MITLRVKDPAELTAL"
     gene            complement(405..1016)
                     /gene="coaE"
                     /locus_tag="VFU45_09195"
     CDS             complement(405..1016)
                     /gene="coaE"
                     /locus_tag="VFU45_09195"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU5359283.1"
                     /translation="MLNIALTGNIGAGKSEVVRRFREWGATVIDADQLAREAQAPGTE
                     VLRAIAQRFGKDVLLPTGELDRAALRGLVMGDDDALAALNGIVHPAVRRRREQLVAEA
                     AARGDTVVVNDIPLLFEAADPAAFDLVVLVDAPAEVRRQRLIRDRDLSAEEADRMIGA
                     QIPSERKRERADLVIENMGTIQDLDRAARAAWDEIRRRAAARA"
     gene            complement(1178..1783)
                     /locus_tag="VFU45_09200"
     CDS             complement(1178..1783)
                     /locus_tag="VFU45_09200"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU5359284.1"
                     /translation="MYSGARSGSIEVVSGVMFSGKSEELIRRVRRAIIARRAVQVFKS
                     HLDARYAGLYHVSSHDGREVEAIPIDSAGEIIRQVRPDTEIVAIDEAQFLDEGIVQVV
                     TTLANRGVRVILAGTDTDFRGEPFGAMGELMAVAEKVDKLRAICVVCGDEACRNQRLV
                     DGRPAKYDSPTIMVGGRESYEARCRHCHRVPRTDEDQTALL"
     gene            2005..2295
                     /locus_tag="VFU45_09205"
     CDS             2005..2295
                     /locus_tag="VFU45_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003215863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU5359285.1"
                     /translation="MNKQELVEAVAKANDTSKAEAAKWVDTFFGHDGVIAKELKKGGE
                     VNVTGFGNFKTRKRAQRTGRNPQTGKTITIKASTAPVFRAGKALKDMVNKKK"
     gene            complement(2413..>3004)
                     /locus_tag="VFU45_09210"
     CDS             complement(2413..>3004)
                     /locus_tag="VFU45_09210"
                     /inference="COORDINATES: protein motif:HMM:NF015817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HEU5359286.1"
                     /translation="PHAEPWLSGVVRGRRPLRLTDTLDRARDEAARSRAQVVARIDAG
                     VPAGALFALAPLFERYDGAEVAGALYELWGAAMAARPAAAPAPVVMPASETGRMWVGV
                     GSIDGVTPNDLVAALVKDLKFERSRIGKIEIREKFSLVELPASDIEKVAAALTGKSVR
                     RRRVVARADRGATPRGESAGSGRPRPAGPRKPRPRS"
BASE COUNT          433 a         1052 c         1096 g          423 t
ORIGIN      
        1 agggccgtga gttcggcggg gtccttgacc cggagcgtga tcatccaccc ggcgccgtac
       61 gggtcgcggt tgacgaccgc cggatcggcg tccagggcgg cgttgacgct caccacctcg
      121 ccggcgaccg gcgtgaagag ctcggagacg gccttgaccg cctcgatggt gccgaacgcg
      181 gcgtgcgcgg cgaactgctg gcccggcttg gggaggttga cgaagacgac gtcgccgagc
      241 tcaccctggg cgtagtcggt gatgccgatc ttgaccacgg cggcgtcggc cgtcttcccg
      301 acgtactcat ggtccttggt gtagcggagg tcggcgggca cgttggacac ggtcactccg
      361 gggcggtgaa ggcgcggtga agataggcgc gctggcgggc cgattcaagc gcgggcggcg
      421 gcacggcggc ggatctcgtc ccaggcggcg cgggcggcgc ggtcgaggtc ctggatggtg
      481 cccatgttct cgatcaccag atcggcccgc tcccgcttgc gctccgaggg gatctgggcg
      541 ccgatcatcc ggtcggcctc ttcggcggag aggtcgcggt cgcggatgag gcgctggcgc
      601 cgcacctccg ccggggcgtc gaccaggacc acgaggtcga acgcggcggg gtcggcggcc
      661 tcgaagagga gggggatgtc gttcaccacc acggtgtcgc cccgggccgc cgcctccgcc
      721 acgagctgct cccgccgccg ccgcaccgcc ggatggacga tcccgttgag cgcggcgagc
      781 gcgtcgtcgt cgcccatcac caggccgcgc agcgccgccc ggtcgagctc gccggtgggc
      841 agcagcacgt ccttgccgaa gcgctgcgcg atggcgcgga gcacctccgt gcccggcgcc
      901 tgcgcctcgc gggccagctg gtcagcgtcg atgaccgtgg cgccccactc gcggaagcga
      961 cggaccacct cggacttccc ggcgccgatg ttgccggtga gggcgatgtt gagcatgggg
     1021 ggaaagctat cagctatcag ctagcagcta tcaggcaact gccgcgccga cggcttgacc
     1081 gggcagggct ccaggcgcac cctcggtctg atagctgata gctgatagct gatggctgat
     1141 agctgatagc tgatagctga tagctgatag ctgatagcta gagaagcgcc gtctgatcct
     1201 cgtccgtccg agggacccgg tgacagtgcc ggcagcgggc ctcgtaactt tcccggccgc
     1261 cgaccatgat ggtgggggag tcgtacttgg cggggcgccc atccaccagc cgctggttgc
     1321 ggcacgcctc gtcgccgcag acgacgcaga tggcgcggag cttgtcgacc ttctcggcca
     1381 cggccatcag ctcgcccatc gcgccgaagg gctcgccgcg gaagtcggtg tcggtgccgg
     1441 ccaggatgac gcgcacgccg cggttggcca gggtggtgac cacctggacg atcccctcgt
     1501 cgaggaactg ggcctcgtcg atggcgacga tctcggtgtc ggggcgcacc tggcggatga
     1561 tctcgccggc ggagtcgatc gggatggcct cgacctcgcg gccgtcgtgg ctggagacgt
     1621 ggtacaggcc ggcgtagcgg gcgtcgaggt ggctcttgaa cacctgcacc gcccgccggg
     1681 cgatgatggc ccgccggacc cggcggatga gctcctcgct cttgccgctg aacatcacgc
     1741 cactgacgac ctcgatcgag ccggagcggg cgccgctgta catcgctgac ctcaggtggg
     1801 ggcgggcggg agcggagagc ttacagggtc ggggcggcgg ggtcaagcga aaaccctgcc
     1861 atcttgcggt tttccgggcg gtgcccccat attgtggcgc gttctcgggc tttcccgccg
     1921 atggacgtcc ggagtcccga tccttccggc gccggcgcgt accgggccga gcaggacaac
     1981 gtcccccctc acacggagtc attcatgaac aagcaggagc tggtggaggc cgtcgcgaag
     2041 gcgaacgaca cctccaaggc cgaggccgcg aagtgggtcg acacgttctt cgggcacgac
     2101 ggcgtcatcg cgaaggagct gaagaagggc ggcgaggtca acgtgaccgg cttcgggaac
     2161 ttcaagaccc gcaagcgggc gcagcgcacc ggccgcaacc cgcagaccgg caagaccatc
     2221 accatcaagg cgtcgacggc gccggtgttc cgcgcgggca aggccctcaa ggacatggtg
     2281 aacaagaaga agtaggcgcg tccggcggac gcccgagggc gcaccgcagc gcgttcgctc
     2341 caccgcaggc caaacgggcc accccgctgg gtggcccgtc ggctttcccg gacccgcgcc
     2401 ggcgtgccgc cgtcaggagc gcggccgcgg cttccggggc ccggcggggc ggggacgccc
     2461 ggacccggca ctctcgcccc gcggcgtggc gccgcggtcg gcccgggcca ccacgcgccg
     2521 gcgccggacg ctcttgccgg tgagcgcggc ggcgaccttc tcgatgtcgc tcgccggaag
     2581 ctccaccagc gagaacttct cccggatctc gatcttcccg atccgcgacc gctcgaactt
     2641 gaggtccttg accagcgccg ccaccaggtc gttcggcgtc acgccgtcga tcgagccgac
     2701 gccgacccac atccggcccg tctcgctggc gggcatgacc accggcgcgg gagcggccgc
     2761 cggccgggcc gccatcgcgg caccccagag ctcgtacagc gcgcccgcga cctcggcacc
     2821 gtcataccgt tcgaacaggg gcgccagtgc gaagagcgcg cctgccggca cgccggcgtc
     2881 gatgcgcgcc accacctgtg cccggctccg cgccgcctcg tcccgggccc ggtcgagcgt
     2941 gtccgtcagc cggagcggac gccgcccccg gacgaccccg ctgagccacg gctcggcgtg
     3001 gggc
//
LOCUS       DASUAP010000340         4675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1305851, whole genome shotgun sequence.
ACCESSION   DASUAP010000340 DASUAP010000000
VERSION     DASUAP010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305851"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2119
                     /locus_tag="VFU45_09215"
     CDS             <1..2119
                     /locus_tag="VFU45_09215"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEU5359287.1"
                     /translation="GVDLLVPMLDLTTDLAAQQGARNVVIGMAHRGRLNVLVHNVGRP
                     YEAIFAEFEGNKPQHDEPDAGTGDVKYHLGAEGVVRATNGKTVSITLSPNPSHLEFTG
                     PVVDGRARAQQTQRKGATAEHDPTAALPVVIHGDAAFAGQGVVAESLNLSKLPGYGTG
                     GTVHIITNNQIGFTTDIKDARSTRYASDLAKGFDIPIVHVNADDPEACLSAVRLLMAY
                     REKFRGDVLIDLIGYRRHGHNEGDEPTYTQPLMYAKIKDHATVRTAYAAALVAQGILT
                     QAEADAQAEAEYQKLVGLQQAMKAAAAKPRSSDPAVTPVSPNAQVDTALPADLLAGLN
                     EQLLAWPAGFTVNLKLRKQLERRRQVLGPDGGIEWAHAEALALGGLLLEGIPVRFTGQ
                     DTERGTFSQRHLVLHDAENGALDSPIKRLHGALAPLELHNSPLSELATMGFEYGYSAA
                     APEALVLWEGQFGDFVNGAQVIIDQFLSAGLSKWGLTTRLTLLLPHGYEGQGPEHSSS
                     RYERFLQLAAEGNIRVANCTTPAQYFHLLRLQAKHPRIRPLVIATPKSLLRLPAATSK
                     LSDLTNGTFQPVIPDPAAPSATRVVLSAGKVYYDLHAEAEKLGAGRPALVRLEQLYPF
                     PDAELKAALALHAKATDIVWVQEEPQNMGAWSFLRERLAAVLPKGATLRYVGRPERAS
                     PAEGYPAAHAKEQKRIVAEALKG"
     gene            complement(2214..2597)
                     /locus_tag="VFU45_09220"
     CDS             complement(2214..2597)
                     /locus_tag="VFU45_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEU5359288.1"
                     /translation="MDAHRDWLLSTYGPVCAYCGQTIKPETATLDHVRPRKGKKAYDR
                     PDNLVIACRDCNAAKADMPLVSWLLQRKSRAVLLLEYGHHLSSGLQQEAFRLVDRPML
                     DLSANDGTPAVVGLRFRPGRPGKRR"
     gene            complement(2797..3360)
                     /locus_tag="VFU45_09225"
     CDS             complement(2797..3360)
                     /locus_tag="VFU45_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5359289.1"
                     /translation="MTKLRRRRWPWVLLLVVGAAGATAWWGRPLALRAVTWKTARRFP
                     GVRWIDADSLARWLAAPRPQPLLLDARTADEVAVSRIAGAVRIDPYRPDLKVLGTPPR
                     DTAIVVYSSVGYRGARVAEWLAGQGYGRVADLAGGIFGWADAGRPVVRVDGPTALVHP
                     FDSRWGLLLTPAHRADAPSLPEESAAP"
     gene            complement(3375..3896)
                     /locus_tag="VFU45_09230"
     CDS             complement(3375..3896)
                     /locus_tag="VFU45_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359290.1"
                     /translation="MSDEPGYLKALRAAQQKKKGVAPVAGGGKHASASVEEREALRWA
                     TEVAKGEVTSTPSVRGAGCVFLFSPRTFTGKAPVVLHGVADRAALAQRVAAAAAAGET
                     VLGCYDVRERKPLTPRGEGGAIVFDAGPARPLPKAVSPEQMIRRATLAAEELAKTRKT
                     DDRDRGGRGGRGR"
     gene            4065..>4675
                     /locus_tag="VFU45_09235"
     CDS             4065..>4675
                     /locus_tag="VFU45_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359291.1"
                     /translation="MTAPSSSAPTPAGPSGPSDALAWATINTIRGLAMDAVEQAQSGH
                     PGTPMALAPVGYVLWSKFLRHDPGDPAWADRDRFVLSCGHASMLLYSLLHLTGYDLTL
                     EDIKRFRQWGSPTAGHPERGHAPGVEVTTGPLGQGVGNCVGMAMAERFLAARFNRPGF
                     DIVDHRTWAFVSDGDLMEGVASEAASLAGHLGLGKICCVYDDNR"
BASE COUNT          706 a         1679 c         1664 g          626 t
ORIGIN      
        1 aggcgtcgac ctgctggtcc cgatgctcga cctcaccacc gacctcgcgg cccagcaggg
       61 cgcgcgcaac gtggtgatcg ggatggcgca ccgcggccgg ctcaacgtgc tggtgcacaa
      121 cgtcggccgc ccgtacgagg cgatcttcgc cgagttcgag gggaacaagc cgcagcacga
      181 cgagcccgac gccggcaccg gcgacgtgaa gtaccacctc ggcgccgagg gcgtggtgcg
      241 ggccacgaac gggaagacgg tgagcatcac gctctcgccc aacccgagcc acctcgagtt
      301 caccggcccg gtggtggatg ggcgggcgcg ggcgcagcag acccagcgca agggagcgac
      361 cgccgagcac gacccgaccg cggcgctgcc ggtggtcatc cacggcgacg cggcgttcgc
      421 gggccagggc gtggtggcgg agtcgctcaa cctgagcaag ctccccggat atgggacggg
      481 cgggaccgtc cacatcatca cgaacaacca gatcggcttc accaccgaca tcaaggacgc
      541 gcgctccacc cgctacgcgt ccgacctcgc caaggggttc gacatcccga tcgtccacgt
      601 caacgcggac gaccccgagg cgtgcctgtc ggcggtgcgg ctcctgatgg cgtaccggga
      661 gaagttccgg ggcgacgtgc tcatcgacct catcggctac cgccggcacg gccacaacga
      721 gggcgacgag ccgacgtaca cgcagccgct gatgtacgcc aagatcaagg accacgcgac
      781 ggtgcggacc gcgtacgccg cggcgctcgt ggcgcagggg atcctgacgc aggcggaggc
      841 ggacgcgcag gcggaggccg agtaccagaa gctcgtcggc ctccagcagg cgatgaaggc
      901 cgccgcggcc aagccccgca gctccgaccc ggcggtcacc ccggtctcgc ccaatgcgca
      961 ggtggacacg gcccttccgg ccgacctcct ggccgggctc aacgagcagc tgctcgcgtg
     1021 gcccgccggc ttcacggtca acctgaagct caggaagcag ctcgagcgac ggcggcaggt
     1081 gctgggcccc gacggcggga tcgagtgggc gcacgcggaa gcgctggccc tcggcgggct
     1141 cctgctcgag gggatcccgg tgcggttcac gggccaggat accgaacgcg gcaccttcag
     1201 ccagcggcac ctggtgctcc acgatgccga gaacggcgcg ctcgactcac cgatcaagcg
     1261 gctgcacggg gcgctggcgc ccctcgagct ccacaacagc cccctctccg aactcgcgac
     1321 gatggggttc gagtacggct acagcgccgc cgcacccgag gcactggtgc tgtgggaagg
     1381 gcagttcggc gatttcgtga acggcgcgca ggtcatcatc gaccagttcc tctccgccgg
     1441 cctttccaag tgggggctta cgacgcggct cacgctcctc ctgccgcacg gctatgaggg
     1501 ccagggcccc gagcactcga gttcgcgcta cgagcgcttc ctgcagctcg ccgccgaggg
     1561 gaacatccgg gtcgccaact gcacgacgcc ggcgcagtac ttccacctgc tccgcctgca
     1621 ggcaaagcat ccgcggatcc gtcccctcgt gatcgcgacg ccgaagagcc tgctccggct
     1681 cccggccgcg acctcgaagc tctccgacct cacgaacggg accttccagc cggtcatccc
     1741 cgatccggcc gctccatcgg cgacgcgggt ggtcctgtcg gcggggaagg tgtactacga
     1801 cctgcacgcc gaggcggaga agcttggcgc cgggcgcccg gcgctggtgc ggctggaaca
     1861 gctctatccc ttcccggacg cggaactcaa ggcggcgctc gcgctgcacg ccaaggcgac
     1921 cgacatcgtc tgggtgcagg aggaaccgca gaacatggga gcgtggagct tcctgcggga
     1981 acggctggcc gccgtgctcc cgaagggcgc gacgctccgc tacgttggcc gccccgagcg
     2041 ggccagcccg gccgaggggt acccggcggc ccacgcgaag gagcagaagc ggatcgtggc
     2101 ggaagcgctg aaaggctgag tggcgggtgg tgggtgcgga cgatccggga agcggaacga
     2161 ccctccgagg cagctctcga agggtcgttc catgtccggg tacacgctac cggtcagcgc
     2221 cgctttcccg gccttcccgg gcggaaccgg agcccgacca ctgccggcgt gccgtcgttg
     2281 gcgctcaaat cgagcatcgg gcggtcgacc agccggaagg cttcctgctg gaggccggag
     2341 gagaggtggt ggccgtattc gagcaggagc accgcgcgcg acttgcgctg cagcagccac
     2401 gacaccagcg gcatgtcggc cttcgcggcg ttgcagtcgc ggcaggcgat gaccaggttg
     2461 tccgggcggt cgtaggcctt cttgcccttg cgcggccgga cgtggtcgag ggtggcggtc
     2521 tccggcttga tcgtctgccc gcagtaggcg cagacggggc cgtaggtgga tagcaaccag
     2581 tcccgatgcg cgtccatcga gcgcgtcgag gaggtacgga aagcggttgc gggaacgtgt
     2641 tccggcgctt ggggccggcg ggagtgggaa cgcctgcggg gcactgatct tccgaacgga
     2701 cgcgtctgac gcgccctttg ggtcgcaccg gtccgcccag tgcgggccgg catacagatt
     2761 aatgatacaa cattcagggt ccgggcgccg ggccccctat ggagcggccg actcttccgg
     2821 cagggacggg gcgtccgccc ggtgggcggg ggtgagcagc agtccccacc gcgaatcgaa
     2881 gggatggacg agcgccgtgg ggccatccac ccggacgacg ggccggcccg catcggccca
     2941 gccgaagatc cctcccgcca ggtcggccac ccggccgtag ccctggcccg ccagccactc
     3001 cgccacgcgg gcgccgcggt atccgaccga actgtacacc acgatggcgg tatcgcgggg
     3061 gggcgtcccg agcaccttca ggtcgggacg gtacggatcg atccgtaccg caccggcgat
     3121 ccggctcacg gccacctcat ccgcggtgcg ggcatcgagg aggagcggct gggggcgcgg
     3181 agcggcgagc caccgcgcca gcgagtcggc gtcgatccac cgcaccccgg gaaagcgccg
     3241 cgccgtcttc caggtgaccg cccgcagcgc gagcggccgg ccccaccacg cggtggcacc
     3301 ggccgcaccg acgaccaaca gcaagaccca cggccagcga cgacggcgga gcttggtcat
     3361 cgtggcccgg cgcgtcagcg cccgcgaccg ccgcggccgc cgcgatcgcg atcgtcggtc
     3421 ttgcgggtct tggcgagctc ttccgccgcc agcgtggcgc ggcggatcat ctgctccggg
     3481 ctcaccgcct tggggagcgg ccgcgccggg ccggcatcga acacgatggc cccgccctcg
     3541 ccacgcgggg tgagcggctt ccgctcccgc acgtcgtagc agccgagcac ggtctcaccc
     3601 gccgccgcgg cggcggccac gcgctgggcg agcgccgccc ggtcggccac gccgtggagt
     3661 acgaccggcg ccttgccggt gaacgtgcgg ggcgagaaca ggaacacgca ccccgcgccc
     3721 cgcaccgacg gggtcgaggt gacctcgccc ttggccacct cggtggccca ccgcagcgcc
     3781 tcccgctcct cgaccgaggc ggaggcgtgc ttgccgccac cggccacggg agcgacgccc
     3841 ttcttcttct gctgtgcggc gcggagcgcc ttcaggtatc cgggttcatc cgacacgatg
     3901 catcgtctcc attcgggccc cgcgggggcc gtgactcgtc cgttcacggg ccaagatagg
     3961 tgggcgggag gagccgggaa agtgcgcggc ggcggtgacg gggaaagtcc ggggtgttag
     4021 tttttgcctc gatttccgct acctcccgct cgccccggag ctccgtgacc gcgccttcct
     4081 cttccgcccc gacccctgcc gggccctccg gtccgtccga tgccctcgcg tgggccacca
     4141 tcaacaccat ccgcggcctg gcgatggacg cggtggagca ggcgcagtcg gggcatcccg
     4201 gcacgccgat ggcgctggcc ccggtcggct acgtgctctg gtcgaagttc ctcagacacg
     4261 atcccgggga cccggcgtgg gccgaccgcg atcgcttcgt gctctcgtgc gggcacgcct
     4321 cgatgctcct ctattccctg ctccacctga cgggatacga cctcacgctc gaggacatca
     4381 agcgcttccg gcagtggggc tccccgacgg cgggccatcc ggagcgcggc cacgcccccg
     4441 gcgtcgaggt caccaccggg ccgctgggcc agggggtggg caactgcgtc gggatggcga
     4501 tggccgagcg gttcctcgcc gcgcggttca accgcccggg gttcgacatc gtcgaccacc
     4561 ggacctgggc cttcgtgagc gacggcgacc tgatggaagg ggtggcctcc gaggcggcga
     4621 gcctcgccgg tcacctgggc ctcggcaaga tctgctgcgt ttatgatgac aaccg
//
LOCUS       DASUAP010000341         4801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_938997, whole genome shotgun sequence.
ACCESSION   DASUAP010000341 DASUAP010000000
VERSION     DASUAP010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938997"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFU45_09240"
     CDS             complement(<1..344)
                     /locus_tag="VFU45_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359292.1"
                     /translation="MAIRTFASRRNSLRLAGFEYSDRRWYFITVCTAGRQPLFGAITP
                     HRVELSAAGHIVEDEWRRSADLRLDVNLDVFIVMPDHFHGLIGLYDSFRAERRSSLPR
                     LIGAFKGAVTRR"
     gene            423..965
                     /locus_tag="VFU45_09245"
     CDS             423..965
                     /locus_tag="VFU45_09245"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5359293.1"
                     /translation="MPLLSSRRLYTGRVLNLDLDGVRFPDGSEGTLEMIRHPGASAVL
                     PCLDDPAAADPRAILIRQFRHAADGFIWEIPAGRLEPAELPVTCAHRELQEETGYRAA
                     TMEPLGTILTTPGFTDERIHLFRATGLTPGAAAREADEFVELHTVPWSRVIEMARTGE
                     IQDAKTLCAIWLADAWRRNA"
     gene            1062..2126
                     /gene="holA"
                     /locus_tag="VFU45_09250"
     CDS             1062..2126
                     /gene="holA"
                     /locus_tag="VFU45_09250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU5359294.1"
                     /translation="MASRAMKAMAAAVKRGAPEPAYYLYGPEDANKDETIRYMVDRLL
                     DPGMRDFNLDQVSAATLDPEAVEALCTTVPMMADRRLVIIRDVEQWKRRTRSRSAVIR
                     YLEHPSPTTVLLLVQGSGEENPDKELLKHATPLECAAATAEEAQAWLEKRAAERGLVF
                     APGAMEHLLRATGADLGAIRIEVEKLGSLAAGTTITTELVGDLVGVRSGETQHDWRDA
                     IMNDDPGRAVTLLPRVLDLPGVSGVRLVSLLGTTLIGVGITRSAFDRRQSGKSLRDAA
                     FSTIRAARLFGLGSWGEEADLWARWAPTWPAPRIRAALADVLAADRALKNTTLSDEHG
                     ILSDMIMRLAQGGGVLEARA"
     gene            2123..3190
                     /locus_tag="VFU45_09255"
     CDS             2123..3190
                     /locus_tag="VFU45_09255"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU5359295.1"
                     /translation="MRRPFIAAVAASALMAVAPAPAAAQSDPRLEAAIALAQEGRRDS
                     AQAAVAAILRTTTPADQLYPEVLYTSALLMTDADSMRRTLQKVVVEHSLSAWADNALL
                     QLAELDFAGGNLPAAMRDLERLRTDFPQSDVFPEAAFWAARTYFDARQENPGCEWVGM
                     GLARVSDSAFAVRDQLQTFARRCPATVLAQGSAKAPAAAPRDTIVLARADTTAKADRA
                     DTTGKADSAMAATVAPPVLDTAVTRRDTVPSAPRDPGVLVARLDTSKATPAPKPAPPP
                     PPARAHAPVYRVQIVAAPTAAAAEGPAQRARAAGLEVRTVNEGGLYKVRVGSYATRAE
                     ASAAARTLRADFPGAFLVTDQ"
     gene            3223..>4801
                     /gene="mutS"
                     /locus_tag="VFU45_09260"
     CDS             3223..>4801
                     /gene="mutS"
                     /locus_tag="VFU45_09260"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU5359296.1"
                     /translation="MQQYREIKARHRDAILFFRMGDFYEMFFDDAELGARVLDITLTS
                     RGDGVPLAGVPVKAAADYLRQLVSAGHRVAICEQVEDPKLAKGIVRREVIETVTPGAL
                     LQDGWVPGGRNNFLVSLSLGAECGLAAVDLSTGEFLLETVPPGGEAEALGRLAPREVV
                     LPTGASLVLDDAVMRTERESWEFDPALAREELARRFGLASLDGLGIGPGDAAAVGAGG
                     ALLRYLADLQPGGLPHLARPVLRRSEGFLWLDDMTRRNLELVEPLRAGARNATLLEAL
                     DATVTAMGARLLRQWILSPLRSPEAIRDRLDAVQVLVNDSRSRERLRDALDGVRDLER
                     LSGRAAAGRATPREMAALRDSLLRLPDLLDALRGLAAREDSAGLRGIEAEFDLLGDLA
                     TELAKALEERPPALLADGGVIRAGHDRELDELRDLRDGGRRYIATLQQRERERTGIAS
                     LKVGFNKVFGYYLEITNAHAARVPEDYERRQTLSGAERYVTPELKEYESKVLGAEERL
                     AVREAQLFGDLRIRAGAE"
BASE COUNT          722 a         1809 c         1588 g          682 t
ORIGIN      
        1 atacggcgcg tcacggcacc cttgaacgcg ccgatcagcc ggggcagcga cgagcggcgc
       61 tccgcccgaa acgaatcata cagcccgatc agcccgtgaa aatgatccgg catcacgatg
      121 aagacatcga gattcacgtc cagccggagg tccgcactgc ggcgccactc atcttcgacg
      181 atgtgccctg cagcgctcag ttcaacccga tgcggcgtga tcgcaccgaa gagcggctgc
      241 cgcccggcgg tgcacacggt aatgaaatac cagcgccgat ccgagtactc gaatccggcg
      301 agtcgcagtg agttgcgccg ggatgcaaag gtacggattg ccatgttcca gcatgaccac
      361 gctgacgcct ggtgatatca tcaaatgccc gcgtgcagtc tcactcttgc gcgattaacc
      421 ccatgcccct cctctcctcc cgccgcctat ataccggccg agtgctgaat ctcgacctcg
      481 acggcgtgcg gttccccgac ggctccgagg gcaccctcga gatgatccgc catcccggcg
      541 cctcggcggt gctgccctgc ctcgatgacc cggctgccgc cgacccgcgg gcgatcctga
      601 tccgccagtt ccgccatgcc gccgacggat tcatctggga gatccccgcc ggccgcctgg
      661 agcccgcgga actcccggtc acctgtgcgc accgcgagct ccaggaggaa accggctacc
      721 gcgccgccac gatggaaccc ctcggcacga tcctcacgac ccccgggttc accgacgaac
      781 ggatccacct tttccgcgcc accggcctca cgccgggcgc cgcggcccgc gaagccgatg
      841 agttcgtcga gctgcacacc gtgccctggt cccgcgtcat cgagatggcc cggaccggcg
      901 agatccagga cgccaagacc ctctgcgcca tctggctcgc tgatgcctgg cgacgcaacg
      961 cctgacccct cccataacca cctgcccgcc tcgcccgccc tgcctgtctt gccgtcctgc
     1021 cgatcctccc gccttcccgt ctttccggct agacttcccc gatggcttcc cgcgcgatga
     1081 aggcgatggc cgccgcggtc aagcgcggcg cgccggaacc tgcctactac ctctacggcc
     1141 cggaagacgc gaacaaggac gagaccatcc gctacatggt ggaccggctg ctcgatccag
     1201 gcatgcgcga cttcaacctg gaccaggtct ccgccgccac cctcgatcct gaagcggtgg
     1261 aggcgctctg caccaccgtg cccatgatgg ccgaccgccg cctcgtcatc atccgcgacg
     1321 tggaacagtg gaagcgccgc acccggtccc ggtccgccgt gatccggtat ctcgagcacc
     1381 cgtcccccac caccgtgctg ctgctggtgc agggctccgg cgaggagaac cccgacaagg
     1441 aactcctcaa gcacgccacg ccgctcgagt gcgcggcagc caccgcggaa gaggcgcagg
     1501 cctggctgga gaagcgcgcc gccgaacgcg gcctcgtgtt cgcgcccggc gccatggagc
     1561 acctgctccg cgccaccggc gccgacctcg gcgccatccg gatcgaggtg gagaagctcg
     1621 gctcgctggc agccggcacc accatcacca ccgaactggt cggcgacctc gtcggcgtgc
     1681 gcagcggaga gacgcagcac gactggcgcg acgccatcat gaacgacgac cccggccgcg
     1741 ccgtcaccct gctgccgcgg gtgctggacc tgccgggcgt cagcggcgtg cggctcgtct
     1801 ccctcctcgg cacgacgctg atcggtgtcg ggatcacgcg ctcggcgttc gaccggcggc
     1861 agtcgggaaa gtcgctccgg gatgccgcgt tcagcacgat ccgcgcggca cggctcttcg
     1921 gcctcggttc ctggggggaa gaagccgacc tctgggcgcg ctgggctccg acgtggccgg
     1981 caccgcgcat ccgggcggcg ctcgccgacg tgctcgccgc tgaccgcgcg ctcaagaaca
     2041 ccaccctctc cgatgaacac ggcatcctgt ccgacatgat catgcgcctg gcccagggcg
     2101 gtggcgtgct cgaggcgcgc gcatgagacg cccgttcatc gccgcggtcg cggccagcgc
     2161 gctgatggcg gtcgcacccg cacctgccgc cgcgcagagc gatccgcggc tcgaggctgc
     2221 aatcgccctt gcccaggaag ggcgccggga ttcggcgcag gcagccgtcg ccgcgatcct
     2281 ccggaccacg acgccggcag accagctcta tccggaagtg ctctacacct ccgccctgct
     2341 gatgaccgac gccgactcga tgcgccgcac cctccagaag gtcgtcgtcg agcactcgct
     2401 ctcggcgtgg gcggacaatg cactgctgca gctcgcggaa ctggatttcg ccggcggcaa
     2461 cctgccggcc gccatgcgcg acctcgaacg cctccgcacc gacttcccgc agtcggatgt
     2521 gttccccgag gcggcgttct gggccgcccg cacctatttc gacgcgcgcc aggagaatcc
     2581 cggctgcgaa tgggtcggca tgggcctcgc tcgcgtctcc gacagcgcgt tcgcggtccg
     2641 ggatcagctc cagacgttcg cccgccgctg cccggccacg gtcctcgcgc aaggcagtgc
     2701 caaggcgccg gcggcggcac cacgtgacac gattgtgctg gcaagggcgg acacgacggc
     2761 aaaggcggac agggcggaca cgacgggcaa ggcggacagc gcgatggccg cgacggtggc
     2821 accgccggtc ctcgacaccg ctgtcacgcg ccgtgacacc gtgccatcag cacctcgaga
     2881 tcccggtgtg cttgttgccc ggctggatac ttcgaaggcg actccggcgc cgaagccggc
     2941 gccaccgcca ccgcccgcaa gggcgcatgc gccggtgtac cgcgtgcaga tcgtcgccgc
     3001 accgacggca gccgcggccg aaggccccgc ccagcgcgcg cgcgcggccg gcctggaagt
     3061 ccgcacggtg aacgagggcg gtctctacaa ggtgcgggtc ggttcctatg ccacccgcgc
     3121 cgaggcctcg gcggccgccc gcaccctccg cgccgatttc cccggcgcat ttctcgtcac
     3181 ggaccagtga ctcgcgcgag cagtgccgaa ggcacgccgc tgatgcagca gtatcgcgag
     3241 atcaaggccc ggcatcgcga tgccatcctg tttttccgca tgggcgactt ctacgagatg
     3301 ttcttcgatg acgcggagct gggggcgcgc gtcctcgaca tcacgctcac ctcgcgcggc
     3361 gacggcgtgc cgctggccgg cgtgccggtc aaggcagcgg cggactatct ccgccagctg
     3421 gtgagcgccg gccatcgagt cgccatctgc gagcaggtgg aggatccgaa gctcgcgaag
     3481 ggcatcgtac gccgggaggt gatcgagacg gtcacgcccg gcgccctgct gcaggacggc
     3541 tgggtccccg gcggccgcaa caacttcctg gtcagcctca gtctcggcgc ggagtgcggg
     3601 cttgccgcgg tggatctctc caccggcgag ttcctgctcg agacggtccc gccgggcggc
     3661 gaggccgagg cgctcgggcg cctggcacca cgcgaagtgg tgctccccac cggcgcttca
     3721 ctcgtgctcg acgacgccgt catgcgcacc gagcgcgaga gctgggagtt cgatcccgcg
     3781 ctcgcccggg aggagctcgc ccgccgcttc gggctcgcct ccctcgacgg cctcggcatc
     3841 ggcccggggg atgcggcagc ggtcggcgcc ggcggcgccc tgctccgcta cctcgccgat
     3901 ctccagcccg gcggcctgcc gcacctcgcg cgcccggtcc tccggcgcag cgagggcttc
     3961 ctctggctcg acgacatgac tcgccgcaat ctcgagctgg tggagccgct ccgcgccggc
     4021 gcccggaacg cgaccctgct cgaggcgctc gacgccaccg tcaccgccat gggcgcccgg
     4081 ctgctgcgcc agtggattct ctcgccactt cggtcaccgg aagcgattcg tgaccggctc
     4141 gacgcggtgc aggtgctggt gaacgacagc cggagccgcg agcgcctgcg cgatgcgctc
     4201 gacggggtgc gggatctcga gcgcctctcc ggccgcgccg ccgccggccg cgccacgccg
     4261 agggagatgg cggccctccg ggattcgctc ctccgcctgc cggacctgct ggatgccctg
     4321 cgtggcctgg cggcacggga ggacagcgcc ggcctccggg gcatcgaggc ggaattcgat
     4381 ctcctgggcg atctcgcgac ggaactcgcg aaggcgctcg aggagcgccc gcccgcgctg
     4441 ctcgccgacg gcggcgtgat ccgggccggg catgatcgtg agctcgatga actccgggac
     4501 ctccgggacg gcggccgccg ctacatcgcc acgctgcagc agcgggagcg ggagcgcacc
     4561 ggcatcgcgt cactcaaggt cggcttcaac aaggtgttcg gctactacct cgagatcacc
     4621 aacgcccacg cggcgcgagt gccggaggac tatgagcggc gccagaccct ttccggcgcc
     4681 gagcgctatg tcacgcccga gctcaaggag tacgaatcga aagtcctcgg cgcggaggag
     4741 cgccttgcgg tccgggaggc gcagctcttc ggggacctgc gcatccgggc aggcgccgag
     4801 g
//
LOCUS       DASUAP010000342         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_979409, whole genome shotgun sequence.
ACCESSION   DASUAP010000342 DASUAP010000000
VERSION     DASUAP010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979409"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFU45_09265"
     CDS             <1..207
                     /locus_tag="VFU45_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEU5359297.1"
                     /translation="GGGPVGLATVAVAVGEDAGTLEEVYEPYLIQQGFLDRTPRGRVA
                     TPQAYRRFGLTPPPGSQASMFEKP"
     gene            210..1298
                     /gene="queA"
                     /locus_tag="VFU45_09270"
     CDS             210..1298
                     /gene="queA"
                     /locus_tag="VFU45_09270"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU5359298.1"
                     /translation="MRTSDFDYELPPDLIAQHPTAERGASRLLVVRRHGPAGPRDGGS
                     TDASTHRAAEPPADALSDRRFSDLIELIPPGDLVVVNTTRVRHARFLGTRPSGAPAEV
                     LLIHPGADGNWLAMGKPGTALRPGKRIRIGPDAEIETVRVDDAGMRHVRFVGLGADAA
                     IARYGRLPLPPYIDRAPDADDESRYQTVYAEREGSVAAPTAGLHFTPALLDQFRARGI
                     AVTGLDLEVGPGTFKPVEGEDITAHPMHPERFEIPAAAAALVADTRRGGGAVWAVGTT
                     VVRALESAAAPGGTVRPGQSETRLMISPGHRFQVVDHLVTNFHLPRSTLLMLVSAFAG
                     HELTMQAYRHAVARRYRFYSYGDAMVIL"
     gene            1298..2491
                     /locus_tag="VFU45_09275"
     CDS             1298..2491
                     /locus_tag="VFU45_09275"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5359299.1"
                     /translation="MFEFTLDGTAGPARAGTLVLPHGTVETPAFMPVGTNGAVRGLSP
                     DEVRDAGARIILGNTYHLHLRPGEDVVRALGGLARFGRWNGPTLTDSGGFQVFSLAEL
                     REITEDGVTFRSPIDGSLRTITPEKSMEIQWALGADIAMAFDHVVPGQASWELAHEGM
                     ERTLRWLDRCKARHAELAEGRSGAGSAGATSAILPAAASLRPSVQTLWPIVQGGTHED
                     LRLRSLEGILEREPWTGIAIGGLSVGEPKPVMHRVLELLGPRLPADRPRYLMGVGFPE
                     DLLEAIARGVDLFDCVAATRNGRHGSAWTADGKVNIRRADLRLSDAPLDAECDCVTCR
                     TYPRAYLRHLFVAEEMLGLRLLSLHNVRFLVRLGEQARQHILRGTFHDWSREWLRRYH
                     GKERA"
     gene            2488..2829
                     /gene="yajC"
                     /locus_tag="VFU45_09280"
     CDS             2488..2829
                     /gene="yajC"
                     /locus_tag="VFU45_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU5359300.1"
                     /translation="MTLPMLTLLAPAPGSPSGGMTILIGQIAVIIGIFYLFIIRPQGQ
                     ARKRHAQLLAGLKKGDDVLTAGGIVGKVKDIKEVADKKEIRVTIESGTSTLVVERSRI
                     IRVGDTSAPQA"
     gene            2829..3362
                     /gene="def"
                     /locus_tag="VFU45_09285"
     CDS             2829..3362
                     /gene="def"
                     /locus_tag="VFU45_09285"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5359301.1"
                     /translation="MPLLPLHLLGSPVLRERSAEVSAFDDELRGFIDSLFETMDANKG
                     VGLAANQVGVARRVAVVDADERRIVLVNPVITAAEGSSREEEGCLSIPEIFGEVTRPE
                     RIVLEALDRDGTPYRLEAGGLLARAIQHEIDHLDGILFLDHLSPLKRQVLVSRYRRDH
                     KGKPVIQETLPATARPE"
BASE COUNT          495 a         1338 c         1215 g          489 t
ORIGIN      
        1 ggcggcggcc cggtcggcct cgccaccgtc gcggtcgccg tgggcgagga cgccggtacg
       61 ctcgaagagg tgtacgagcc ctacctcatc cagcagggct tcctcgaccg cacgccgcgg
      121 ggccgggtcg ccacgcccca ggcgtaccgc cggttcgggc tgaccccgcc accgggctcc
      181 caggcgtcga tgttcgagaa gccgtagcgg tgcgcacttc cgatttcgac tacgagctcc
      241 cgcccgacct catcgcgcag caccccaccg ccgaacgggg ggcatcgcgg ctcctcgtgg
      301 tgcggaggca cggaccggca ggaccgcggg acggcgggtc gacggatgca tcgacccacc
      361 gagctgccga gccgccggcc gacgccctct ccgatcgccg attctccgac ctcatcgagc
      421 tcatcccccc gggcgacctc gtcgtcgtca acaccacccg ggtgcgacac gcgcgcttcc
      481 tcggcacccg cccctcgggt gcaccggcgg aggtgctgct catccacccc ggcgccgacg
      541 gcaactggct cgcgatgggc aagccgggca ccgcgctccg ccccggcaag cggatccgca
      601 tcgggcccga cgccgagatc gagacggtac gggtggacga cgccgggatg cggcacgtgc
      661 ggttcgtggg cctcggggcg gacgccgcca tcgcgcgcta cggccgactc ccgctgcccc
      721 cctacatcga ccgcgcgccc gatgccgacg acgaatcccg ctaccagacc gtgtacgccg
      781 agcgcgaagg gagcgtggcg gcgccgacgg cgggcctcca cttcacgccg gcgctgctcg
      841 accagttccg ggcccgcggc atcgccgtga cggggctcga cctcgaagtg ggccccggca
      901 ccttcaaacc cgtcgaaggc gaggacatca ccgcgcaccc gatgcacccg gagcggttcg
      961 agatcccggc cgccgcggcc gcgctggtgg cggatacccg ccggggcggc ggcgcggtgt
     1021 gggccgtcgg caccaccgtg gtccgcgcgc tcgagagtgc cgccgccccc ggcgggaccg
     1081 tccggcccgg gcagtcggag acccggctca tgatctcccc cgggcaccga ttccaggtgg
     1141 tggatcacct cgtcacgaac ttccacctcc cccgttccac gctgctgatg ctcgtgtcgg
     1201 cgttcgcggg gcacgagctc acgatgcagg cgtatcgcca cgccgtggcc cggcgctacc
     1261 gcttctactc ctatggtgac gcgatggtga tcctctgatg ttcgagttca ccctcgacgg
     1321 caccgccgga cccgcccgcg ccgggacgct cgtcctcccg cacggcacgg tggagacccc
     1381 ggcgttcatg cccgtgggca ccaacggcgc cgtgcgcggg ctctcgcccg acgaggtccg
     1441 cgacgccggc gcccggatca tcctgggcaa cacgtaccac ctccacctcc gccccggcga
     1501 ggacgtcgtc cgggcgctgg gcggcctggc ccgcttcggc cggtggaatg gcccgacgct
     1561 cacggactcg ggcgggttcc aggtcttctc gctcgccgaa ctgcgcgaga tcaccgagga
     1621 cggcgtgacc ttccggagcc ccatcgacgg ctccctccgc acgatcacgc ccgagaagtc
     1681 gatggagatc cagtgggcgc tcggggcgga catcgcgatg gcgttcgacc acgtcgtgcc
     1741 gggccaggcc agctgggagc tggcccatga agggatggag cgcacgctcc gctggctcga
     1801 ccggtgcaag gcacggcacg cggaactggc ggaaggacgc agcggcgcag ggtcggcggg
     1861 ggcgacgtcc gccatcttgc ccgccgctgc gtccctccgc ccctccgtcc agaccttgtg
     1921 gcccatcgtc caggggggca cccacgagga cctgcgcctc cgatcgctgg aggggatcct
     1981 cgagcgcgag ccgtggaccg gcatcgccat cggcgggctg tcggtgggcg agcccaagcc
     2041 ggtgatgcac cgcgtgctcg agctcctcgg cccgcggctc ccggccgatc gaccccgtta
     2101 tcttatgggg gtcggcttcc ccgaggacct gctcgaagcg atcgcgcggg gcgtggatct
     2161 cttcgattgc gtggccgcca cacggaacgg gcgccacggc agcgcgtgga ccgcggacgg
     2221 caaggtgaac atccgccggg ccgacctgcg cctgtcggac gcgccgctcg acgccgaatg
     2281 cgactgcgtg acctgtcgga cctatccgcg ggcctacctc cgccacctgt tcgtggccga
     2341 ggagatgctg ggcctccggc tgctgtcgct gcacaacgtc cggttcctcg tgcgcctcgg
     2401 tgaacaggcg cggcagcaca tcctgcgggg gaccttccac gactggagcc gcgagtggct
     2461 ccggcgctac cacggcaagg aacgcgcgtg accctcccga tgctgacact gctcgccccc
     2521 gctcccggca gcccctccgg cgggatgacc atcctgatcg gccagatcgc cgtcatcatc
     2581 ggcatcttct acctcttcat catccggccg cagggccagg cccggaagcg ccacgcgcag
     2641 ctgctggcgg ggctgaagaa gggtgacgat gtcctcaccg caggcgggat cgtcggcaag
     2701 gtgaaggaca tcaaggaagt ggccgacaag aaggagatcc gcgtgaccat cgagtcgggc
     2761 acctcgaccc tggtggtcga gcgctcccgc atcatccgcg tcggcgacac gagcgcgccc
     2821 caggcctgat gcccctcctg ccgctccacc tcctcggatc gccggtgctc cgggaacgga
     2881 gcgccgaggt ctcggcgttc gacgacgagc tgcgcggatt catcgactcg ctcttcgaga
     2941 cgatggacgc caacaagggc gtggggctcg ccgccaacca ggtcggcgtg gcgcggcggg
     3001 tggcggtggt ggacgcggac gagcggcgca tcgtgctcgt caacccggtc atcaccgccg
     3061 ccgaagggag ctcacgggag gaggaggggt gcctctcgat ccccgagatc ttcggtgagg
     3121 tcacgcggcc ggagcggatc gtcctcgagg cgctcgaccg ggacggcacg ccgtaccggc
     3181 tcgaggcggg cgggctgctg gcgcgggcca tccagcacga gatcgaccat ctcgacggca
     3241 tcctcttcct tgaccacctg agcccgctca agcgccaggt gctggtgtcg cgttaccgtc
     3301 gggaccacaa gggcaagccc gtcatccagg agacgctgcc cgcgacagcg cgcccggagt
     3361 gatgcggatc gtcttcttcg gcacgccgga gttcgccgtc ccgtccctcc gcgcgctcct
     3421 cgccgaggcc gacacgttca cggtcgcggg cgtcgtcacc cagcccgacc gtccccgcgg
     3481 ccggtcccgg tccacgctca tccccccgcc ggtcaaggcc gcggccctcg aggccgg
//
LOCUS       DASUAP010000343         5494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_215982, whole genome shotgun sequence.
ACCESSION   DASUAP010000343 DASUAP010000000
VERSION     DASUAP010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215982"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFU45_09290"
     CDS             <1..890
                     /locus_tag="VFU45_09290"
                     /inference="COORDINATES: protein motif:HMM:NF028211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5359302.1"
                     /translation="THAMVRRERIGHTAAITLTSAIDGRVMFVLPWGELSYIGTTDTD
                     TDEPPEAVRATEEDTRYLLRSANAMFPNAHLTPEDVIATWAGLRPLLADPGAESTAAV
                     SREHAIVRGPGGMLTIAGGKLTTHREMAAQLVDRIGDEFHALEARRRPPRAPTDREPL
                     PGGESARLDPFRTEAMDLGLDAASAEHLLQHYGSETPAVLNLVRGDRALGRRLVAGHP
                     AIGATVIHAARREYATTVEDVLVRRIHLYYETGDQGCAAASAVAELLGRELAWEPGAA
                     ARAAGAYVARVRSGGFSEE"
     gene            complement(877..1971)
                     /locus_tag="VFU45_09295"
     CDS             complement(877..1971)
                     /locus_tag="VFU45_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5359303.1"
                     /translation="MKWLAGVLLLACGACSSRSNACPVVALAGATPHSVAGEVASAVL
                     AAEPRGRRVCIEVFRHRSYDSVGDPAAEVAMAERVAAQDDVMAVVGHATSSGSLAAAP
                     VYRSAGITQLVPTATSRLLAQFRPSAMSLAPDDSLEGLALAAFGAQALRAHRAFVFYV
                     ANEYAEGLLGGLRVGLAGRNVAVTAAARILPESDLPTLVDAAIRRQQADIAYLIGDYR
                     TVGRLAQVLHARRRTLPLVAGDAALYPEGLAEQAGEALPSLRVVGFQRNGEDSARSAA
                     YLAAFRRIAGREPTPDEMLTHDALMVAVAAVRTAGPDRAAVESWLASLGGSRPAWRGV
                     TGPVQFAHPADVRFGVFRIQDGIAVPVTPR"
     gene            complement(1968..3914)
                     /locus_tag="VFU45_09300"
     CDS             complement(1968..3914)
                     /locus_tag="VFU45_09300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEU5359304.1"
                     /translation="MSLPALGKDAAGPHTIRLRTLLLILLGAIGVYVLGIFLYLTLHV
                     GREARTLQRDVEPALYVFRQVVARGRELDDAVVAAHLLTAHRGVDTVGQAAALALIQA
                     RATEPIRSLPFAGVPDSMRPSLAQADEEVSLLQNKLLEVAALVELRRFPEAERRLLQV
                     DSLNRGLSDAIAAAEDGGLSDLLRREQLLRSAIDSVVLALGWWIVVGILFLPLVLFVL
                     HQRVERPLAELEGALGRVAEGELGTRVPVRRHDEIGRLAGHFNVMTQVLRERATEQGR
                     FAAAGVLLADVAHEVNNPLMAIGALAETRLQDPALDDDQRKETEQILRQARRAGRLLS
                     GLLRFVRPAEIRVSAIAVNEVVRGALDLVAHQFGAGGVMVDLRLDPVSPQTLGDPNRL
                     EQVFVNLLSNALDAMRGLPGPRVLSVETWVADGRVHAAVADTGPGVSGAVAEQLFRPF
                     VSTKGKQGTGLGLYISRQIVRETGGDLVLASPERGVGARFVASFPMAEPGAANVDALR
                     HATPPPAGALRGQPLLGLRILVVEDEESVRRPVARFLAQRGATVDEAAHGVEGLARIG
                     NAEPDVIVADIRMPVMDGITFFRRLVAEHPDLSRRVVFFSGDFSQLSQLEGLTIPPDR
                     QLLKPVDLHLLEDRVRSIARGRRA"
     gene            complement(3911..4987)
                     /locus_tag="VFU45_09305"
     CDS             complement(3911..4987)
                     /locus_tag="VFU45_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5359305.1"
                     /translation="MAAILLAATACEVHRSRPMIALSYPAFAEPYAAVAAALARPGPS
                     GAERPRILYDTADVQEVSEAMVAWAQRVGRMREVVGVVGPSGSRVALATAPVYNDAGI
                     PQVVPMATDRLLAQSGPWTFTMAPDDSVEGAFLADYVDRVVRAQRVLLFYENDEFGQS
                     LRGSLLREFDARGIRVLAQVSVFPNSDFEPLLVAALTSGRPDAVVMATRETEAGAIAN
                     LGASRLPRVPMIAADGALVPQRLVVLAGAGLANLRVVAFWVPDTTDARQRAFIAETRR
                     VTGHDPNADLAMIQDGIDLMSAAIDDVGPDRTAIRDWLRSLGRERPAFPGLTGPISFG
                     TGRASTLTMVRVAPDGSIAPEPVR"
     gene            complement(5132..>5494)
                     /locus_tag="VFU45_09310"
     CDS             complement(5132..>5494)
                     /locus_tag="VFU45_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5359306.1"
                     /translation="GSSTGAVTVTATAAGLVGSPVTFGETIAHSALFTVQVGNDTFTS
                     DSLIISAGDQVKWVWGPTAVTHNVTSDPSPSFPSSTNLGANATYGPITFTTPGIYYYH
                     CTIHGTTRSGMYGVIVVQ"
BASE COUNT          812 a         2008 c         2014 g          660 t
ORIGIN      
        1 gcacccacgc gatggtgcgc cgcgagcgca tcggccatac ggcggcgatc accctcacga
       61 gcgccatcga cggccgggtg atgttcgtgc tcccgtgggg ggaactctcc tacatcggca
      121 ccaccgacac cgacaccgac gagccccccg aggcggtgcg cgcaaccgag gaggacaccc
      181 gatacctcct ccgctccgcg aacgcgatgt tcccgaatgc gcacctcacg ccggaggacg
      241 tcatcgcgac gtgggccggc ctccggccgc tcctggccga cccgggggcg gagagcacgg
      301 cggcggtctc gcgcgagcac gcgatcgtcc gcgggcccgg cgggatgctg acgatcgcgg
      361 ggggcaagct caccacccac cgggagatgg cggcgcaact ggtcgaccgc atcggcgacg
      421 aattccacgc gctcgaggcg cggcgccgcc caccgcgcgc gccgaccgac cgcgagccgc
      481 tgcccggggg tgagtccgcc cgccttgacc cgttccgcac cgaggcgatg gacctgggac
      541 ttgacgccgc ctcggccgag cacctgctgc agcactacgg cagtgagacc ccggcggtgc
      601 tcaacctcgt gcgcggcgac cgcgcgctcg ggcgccgact ggtggccggg cacccggcga
      661 tcggcgccac ggtgatccac gcggcccggc gcgagtatgc caccacggtc gaggacgtcc
      721 tcgtgcggcg gatccacctc tactatgaga cgggcgacca gggatgcgca gcggcgtcgg
      781 cagtggccga gttgctgggc cgcgagcttg catgggaacc gggcgccgcg gcccgcgcgg
      841 ccggcgccta cgtggcgcgg gtacggagcg gcgggttcag cgaggagtga ccgggacggc
      901 gatgccgtcc tggatgcgga acacgccgaa gcggacatcg gcaggatggg cgaactgcac
      961 cgggccggtg acgccgcgcc acgcaggccg tgagccgccg agggacgcga gccacgactc
     1021 gacggcggcg cgatcggggc ccgccgttcg cacggccgcc acggccacca tcagtgcgtc
     1081 gtgcgtgagc atttcgtcgg gtgtcggttc gcgcccggcg atgcggcgga acgccgcgag
     1141 gtatgcggcc gaccgcgcgg agtcctcacc gttccgctgg aagcccacca cccgcagtga
     1201 cgggagcgcc tccccggcct gctccgccag cccctcgggg tagagtgccg cgtcgccggc
     1261 cacgagcggg agcgtccgcc gccgggcatg gagcacctgt gccagccgcc ccacggtgcg
     1321 gtaatcgccg atcaggtatg cgatgtccgc ctgctggcgg cggatggcgg cgtcgaccag
     1381 ggtgggcaag tcgctttccg gcaggatgcg ggcggcggcg gtgaccgcca cattccggcc
     1441 ggccaggccc acccgcaggc cgcccagcaa cccctccgcg tattcattcg cgacatagaa
     1501 cacgaacgcc cggtgcgcgc gcagcgcctg tgcgccgaag gcggccagcg ccagtccttc
     1561 gagcgaatcg tccggggcca ggctcatggc cgacgggcgg aactgcgcca gaagccggct
     1621 cgtcgccgtc ggcaccagct gcgtgatgcc cgcgctgcgg tagaccggcg ccgccgcgag
     1681 gctcccgctg ctggtggcgt gccccaccac ggccatcacg tcatcctgcg cggccacccg
     1741 ctcggccatc gccacttcgg cggcgggatc tccgaccgaa tcgtacgacc ggtggcggaa
     1801 cacctcgatg cagacccggc gcccccgcgg ctccgcggcc agcacggccg aggcgacctc
     1861 gcccgcgacc gagtgcggcg tggcgcccgc cagcgccacg accgggcacg cattggagcg
     1921 ggaggagcag gccccgcatg cgagcagcag gacacccgcc agccacttca cgctcggcgc
     1981 ccccgggcga tcgaccggac ccggtcctcg agcaggtgca ggtcgacggg cttgaggagc
     2041 tggcggtccg gcgggatcgt gagcccctcg agctgcgaga gctggctgaa atcgcccgag
     2101 aagaacacca cccggcgtga caggtcggga tgctcggcca cgaggcggcg gaagaacgtg
     2161 atgccgtcca tcaccggcat ccggatgtcc gccacgatca cgtccggctc ggcgttgccg
     2221 atccgggcga gcccttcgac cccgtgcgcc gcctcgtcga ccgtggcgcc gcgctgggcc
     2281 aggaaccgtg ccaccggccg tcgcaccgac tcctcgtcct cgaccacgag gatccgcagc
     2341 cccagcagcg gctggccgcg gagcgcgccc gcggggggcg gggtggcgtg gcgcagggcg
     2401 tccacattcg cggcgccggg ctccgccatc gggaagctgg ccacgaaacg cgcgcccact
     2461 ccgcgctccg gcgatgcgag cacgagatcg cccccggtct cgcggacgat ctggcgggag
     2521 atgtagagcc cgagcccggt gccctgcttg cccttggtcg acacgaacgg gcggaagagc
     2581 tgctcggcga cggcgccgct cacgccggga cccgtgtcgg cgaccgcggc atggacgcga
     2641 ccatcggcca cccacgtctc cacgctcagc acccgcgggc ccgggaggcc gcgcatcgca
     2701 tcgagcgcgt tgctcagcag attgacgaac acctgctcca ggcggttcgg gtcgccgagc
     2761 gtctgggggg acaccgggtc gagccgcagg tcgaccatca cgccgccggc gccgaactgg
     2821 tgcgcgacga ggtccagggc gccgcggacg acctcgttga ccgcgatggc cgagacgcgg
     2881 atctcggccg ggcgcacgaa gcgcagcagg ccggagagca gccgccccgc ccggcgcgcc
     2941 tgccgcagga tctgctcggt ctccttgcgc tggtcgtcgt ccagcgccgg gtcctgcagg
     3001 cgggtctcgg ccagcgcgcc gatggccatg agggggttgt tgacctcgtg ggccacgtcg
     3061 gccaggagca cgccggcggc cgcgaagcga ccctgctccg tggcccgctc gcggaggacc
     3121 tgggtcatca cgttgaagtg gcccgccaga cggccgatct cgtcgtggcg ccggaccggg
     3181 actcgggtgc cgagctcgcc ctcggcgacc cgcccgagcg caccctccag ctcggccagc
     3241 gggcgctcca cccgctggtg cagcacgaac agcacgagcg ggaggaagag gatccccacc
     3301 acgatccacc agcccagggc cagcactacg ctgtcgatcg cggagcgcag gagctgctcg
     3361 cgccgaagca ggtccgagag cccgccgtcc tcggctgccg cgatggcgtc gctcagtccc
     3421 cggttcagcg aatcgacctg gagcagccgg cgctccgcct cggggaaccg gcgcagttcc
     3481 accagcgccg ccacctccag gagcttgttc tggaggagcg agacctcctc atccgcctgc
     3541 gccaggctcg ggcgcatgct gtccggcacc cctgcgaagg ggagcgagcg gatgggctcg
     3601 gtcgcgcgcg cctggatgag cgcaagcgcg gcggcctgcc cgacggtgtc cacgccgcga
     3661 tgcgcggtca ggagatgcgc ggcgacgacg gcgtcgtcga gctcccggcc ccgcgccacg
     3721 acctggcgga agacgtagag cgccggttcc acgtcgcgct ggagcgtgcg cgcctcgcgg
     3781 ccgacgtgga gcgtgaggta gaggaagatc ccgaggacgt agacgccgat ggcaccgagg
     3841 aggatgagca gcagcgtgcg gagccggatg gtgtgcgggc cggcggcatc cttgccgagc
     3901 gcggggaggc tcatcgcacc ggctcgggcg cgatgctgcc gtcgggtgcg acccgcacca
     3961 tcgtgagcgt ggacgcgcgg ccggtgccga atgagatggg ccccgtcagc cccggaaatg
     4021 ccgggcgctc ccggccgagg gaccggagcc agtcccggat cgcggtgcgg tccggcccca
     4081 cgtcgtcgat cgcggcggac atcaggtcga tcccgtcctg gatcatggcg aggtcggcgt
     4141 tcgggtcgtg gccggtgacg cgccgggtct ccgcgatgaa cgcgcgctgc cgcgcgtcgg
     4201 tggtatcggg gacccagaac gccaccacac ggaggttggc caggccggcg cccgcgagca
     4261 ccaccaggcg ctggggcacg agggcgccgt cggccgcgat catgggcacg cgagggagac
     4321 ggctcgcgcc gaggttcgcg atcgcgcccg cctcggtctc ccgcgtggcc atcacgacgg
     4381 cgtcgggccg gcccgaggtg agcgcggcga ccaggagcgg ctcgaagtcg ctgttgggga
     4441 agacactgac ctgtgcgagc accctgatcc cgcgcgcgtc gaactcgcgc agcagcgacc
     4501 cgcggaggct ctggccgaac tcatcgttct cgtagaacag caggacccgc tgggcgcgca
     4561 ccacccggtc cacgtagtcc gccaagaacg cgccttcgac cgagtcgtcg ggcgccatcg
     4621 tgaaggtcca cggtcccgac tgggccagca ggcggtccgt cgccatcggc accacctggg
     4681 ggatccccgc atcgttgtac accggcgccg tcgcgagcgc cacgcgactc cccgacgggc
     4741 cgaccacccc gacgacctcc cgcatccggc cgacgcgctg cgcccaagcc accatcgctt
     4801 ccgagacctc ctggacgtcg gcggtgtcgt agaggatgcg cggccgctcc gcccccgaag
     4861 gccccgggcg cgccagcgcc gccgcgacgg ccgcatacgg ctccgcgaac gccggatagc
     4921 tcagggcgat catcgggcgc gaccggtgga cctcgcaggc cgtcgcggcc agcaggatcg
     4981 ctgccatcga gcacgcgcga acggacatcc gccaccggga agggagggag ttcgggaatt
     5041 cgaccgacag gaagatgcta cgccccggcg ggggggccag caagggcgac ccgcccggca
     5101 ccggaccgat gccgggaggg caccccgggg ttcactgcac cacgatcacg ccgtacatcc
     5161 cgctccgggt ggtgccgtgg atggtgcagt ggtagtagta gatgccgggc gtggtgaacg
     5221 tgatcggccc ataggtcgcg ttcgcgccga ggttggtact gctgggaaag ctcggcgagg
     5281 ggtcggaggt cacgttatgg gtgaccgccg tggggcccca tacccacttg acctgatcgc
     5341 cggcgctgat gatcaggctg tcggaggtga aggtgtcgtt acccacctgc accgtgaaga
     5401 gcgccgaatg cgcgatcgtc tccccgaagg tcaccggcga gccgaccagg cccgcggccg
     5461 tcgcggtgac ggtcacggcg ccggtggaag atcc
//
LOCUS       DASUAP010000344         5659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_979482, whole genome shotgun sequence.
ACCESSION   DASUAP010000344 DASUAP010000000
VERSION     DASUAP010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979482"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFU45_09315"
     CDS             complement(<1..362)
                     /locus_tag="VFU45_09315"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5359307.1"
                     /translation="MPHFDLIVIGSGPAGEKGAAQAAYFGKRVLLIERSAELGGAGIN
                     TGTIPSKTLRETALYYSGLRQRGLYGLDYSLKEEMTIADFMFRKDHVVANERALVVRN
                     LDRHGVTVLHGTASFVDRR"
     gene            complement(590..2149)
                     /locus_tag="VFU45_09320"
     CDS             complement(590..2149)
                     /locus_tag="VFU45_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU5359308.1"
                     /translation="MTGSRPAPGKWTITGTVLTGTMIAVLDSSIVNVALPNMAGTLGA
                     TIEEITWVVTAYILANVIIMPIIGMLSAHLGRKNIYLASVVTFTLASMACGAARSMPF
                     IIFFRVLQGLGGGVLITVSQAILRETFPAEEQGLAMGIYGMGVVLAPAFGPTLGGWLT
                     DTYSWPWIFYINVPIGIINIILVQRYIFDPPYLSHDKGPIDFLGVGLLTVGLGSFQLM
                     LEKGQEKDWFDSSFIVWLAFIAAVGMAVFIWRELETDHPAVDLRLLTNRPFTAATSLG
                     GILGMGLYASLFILPLFLQNNLGYPAMKSGLALMPRSLAMAVGMPLAGRFYNKLGPKV
                     LIAAGLLVSAYSFWALGQLSPDTGFWDIFIPQMWQGVGFSLLFVALSTAALATIERRQ
                     MTAAAGLYNVVRQVFGSIGIAIAATQITAGVDRSYAVLSEHVTAYDPATRHWLAAVTA
                     GMMRRFGTDLAMAREKALALLHGSLMSQAAVIAYNRVFLLIAISFILALPLVLLITAG
                     DSTEAVEVMVD"
     gene            complement(2146..2613)
                     /locus_tag="VFU45_09325"
     CDS             complement(2146..2613)
                     /locus_tag="VFU45_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU5359309.1"
                     /translation="MKVMADAVFETSLLVTRLVRAEARQSRPAGLSLSEFRALACLGT
                     YPDCSLTDLAEYLGLQLPTASKLADLLVRRGQITRREDAADRRRNRLALTPPGRAKLD
                     LAMAAVREHLQSRLSGLSSADQQVVVRAMQILGPLVRPAAPPSDASHAAVPAP"
     gene            2694..4541
                     /locus_tag="VFU45_09330"
     CDS             2694..4541
                     /locus_tag="VFU45_09330"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5359310.1"
                     /translation="MRPSLAVPALLLLPTLLGAQNPLARPTDGVEVRYVTSQPVVQYV
                     IHVDSTDLSGFQVELRLSALPDTFRLAMARHPEYDDRFWRYLKGLRVETAGGPGVVTR
                     LDDALWQVVAPGGRAIVRYRIQLPAPQPLRAAWKPFLARDGGLIGGTQSLLYIVGATL
                     APAVVSLDLPDGWNAATGLVPTSDARTFFAPTVGILVDSPILIGKFSDRHFFVDGVPH
                     RIAYWSLPDGTPFDTVTFSRGIESVVRQAIAIFGRAPYREYTFLLQDNAYGALEHLNS
                     LTLGAPSAELAKDPTGLLEEIAHEYFHTWDLLRIRLAGAGDVTWRAPRPSSGLWVGEG
                     FAMYYADLLLRRAGLPIEDSARTDHLARLMEQYLAREDGYTRYTAEQVSQVANTVPGN
                     LGDYLLSTHLQGELLGAMLDLRIRAATHDEHSLDDVMRRMMERFSADSGFSTHDVEAV
                     ASEVCGCNLKAFFRAYVRGRKPIPFDQYLRLIGMHSEVSWAPARNDSGAERPDLRVTA
                     WMPPEAKHPSLMLFTPQGIWGKAGLHTGDQVLRIDGAPIADAAAFRAALGRLAIGQPA
                     QLAIERDGKTIGIRVVIRGYDAPGVTIVPNAHSTADQRALRTAWLSGGQ"
     gene            4542..>5659
                     /locus_tag="VFU45_09335"
     CDS             4542..>5659
                     /locus_tag="VFU45_09335"
                     /inference="COORDINATES: protein motif:HMM:NF020120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5359311.1"
                     /translation="MLFLLLVAQAPSPRSPARAVALEVEHLRTPLGIDATRPRFSWQM
                     SDPRPGARQRAYEILVASSPERLTPARADLWTSGVVRSSATLDVPYAGTALAPMRRYY
                     WTVCLRDREDRPAPCAPASWFETGRMAMPWQARWIAASRAMLDTLAHDPLEGDTAGTD
                     GRPPLLRKDVVLAARPVRARIYATALGSYRLSINGRPVSRAVFTPDWTDYRYRVTYQT
                     YDVTSLLHTGPNALGVLLGAGWYASRFGFSSKRYAYGPPPVRFLLELHLTYADGHTEV
                     VTSDSSWQTAPSPVLISEIYDGETYDARLEQPGWDRPGFDAESWRHAVELPPVKVTIQ
                     PQAMPPIEETDSVRPVRRWSPGAGVYIFDLGQNIAGWAR"
BASE COUNT          907 a         2014 c         1919 g          819 t
ORIGIN      
        1 cgccggtcga cgaacgacgc cgtcccgtgg agcaccgtga ccccgtggcg atcgaggttc
       61 cgcaccacca gcgcccgctc attggcgacc acgtgatcct tgcggaacat gaagtccgcg
      121 atggtcatct cctccttgag cgagtagtcg aggccgtaga gcccgcgctg gcggaggccg
      181 gagtagtaaa gggccgtctc ccggagcgtc ttcgacggga tggtcccggt attgatcccg
      241 gccccaccca gctcggccga ccgttcgata aggagcaccc gcttcccgaa atacgcggcc
      301 tgcgccgccc ccttctcgcc cgcggggccc gatccgatca cgatgaggtc gaagtggggc
      361 atctgcgaag ggaagaggag aaggaggagg agaaggagga agtgagaagt aagaagtgag
      421 aagtgagaag ttagaagtga gaagtgagaa gtgagaaggg gggacgtgag tcggaagtct
      481 ggaccttgga gtctagagac ctgcgacccg tgacccgcga cccgcgaccc gcgacccgcg
      541 acccgcgacc cgcgacccgc cctgatgata cggcccaccg gacgctccgc tagtccacca
      601 tcacctcgac cgcttcggtg gagtcgccgg cggtgatgag gagcaccaac ggaagtgcga
      661 ggatgaacga gatcgcgatg aggaggaaca ccctgttgta ggcgatcacc gcggcctgcg
      721 acatcaggct cccgtgcagg agcgccagcg ccttttcccg ggccatcgcg aggtcggtgc
      781 cgaagcgacg catcatcccc gcggtcaccg cggcgagcca gtgccgcgtg gcggggtcgt
      841 aggcggtcac gtgctccgac agcacggcgt acgaacgatc gacacccgcc gtgatctgag
      901 tggcggcgat ggcgatcccg atgctcccga acacctggcg caccacgttg tagagcccgg
      961 cggcggcggt catctggcgc cgttcgatgg tggcgagcgc cgccgtcgag agcgccacga
     1021 agaggaggct gaaccccacc ccctgccaca tctgggggat gaagatgtcc cagaacccgg
     1081 tatcggggct cagctgcccc agcgcccaga acgaatacgc gctcacgaga agccccgcgg
     1141 cgatgaggac cttgggcccg agcttgttgt agaaccggcc ggcgagcggc atccctaccg
     1201 ccatcgccag gctccgcggc atcagcgcca gtcccgattt catcgccggg tagccgaggt
     1261 tgttctgcag gaagagcggc aggatgaaga ggctggcgta gagccccatc ccgaggatcc
     1321 cgccgaggga cgtcgccgcg gtgaacggcc ggttggtgag taatcggagg tccaccgcgg
     1381 gatggtcggt ctcgagctcg cgccagatga agacggccat ccccacggcg gcgatgaagg
     1441 cgagccagac gatgaacgag gagtcgaacc agtccttttc ctggcccttc tcgagcatca
     1501 gctggaacga gccgagcccc acggtcagga ggccgacgcc gaggaagtcg atcgggccct
     1561 tgtcgtggct caggtacggc ggatcgaaga tgtagcgctg gaccaggatg atgttgatga
     1621 tgccgatcgg cacgttgatg tagaagatcc agggccacga ataggtgtcg gtgagccagc
     1681 cgccgagggt gggcccgaag gccggcgcca gcacgacacc cattccgtag atccccatcg
     1741 ccagcccctg ctcctcggcc gggaaggtct cccgcaggat cgcctgcgag acggtgatga
     1801 gcaccccgcc acccagcccc tgcagcaccc ggaagaagat gatgaagggc atgctcctgg
     1861 cggcgccgca cgccatcgac gccagggtga acgtgaccac gctcgccagg tagatgttct
     1921 tccgcccgag gtgggcggag agcatcccga tgatcggcat gatgatcacg ttggccagga
     1981 tgtaggccgt gaccacccag gtgatctctt cgatggtggc accgagggtg ccggccatgt
     2041 tggggagcgc cacgttgacg atgctcgagt cgagcaccgc gatcatcgtc ccggtgagga
     2101 cggtcccggt gatggtccac ttgccgggag cggggcggga gccggtcacg gcgcgggaac
     2161 ggctgcgtgc gacgcgtcgg aaggcggtgc cgccgggcgc accagcggcc cgaggatctg
     2221 catcgcccgg accaccacct gctggtcggc ggacgacagt ccgctcaggc gcgactgcag
     2281 gtgctcgcgc accgccgcca tcgcgaggtc gagcttggcg cgtccaggcg gtgtgagggc
     2341 cagccggttg cggcgccggt cggccgcgtc ctcccgccgg gtgatctggc cgcgacggac
     2401 cagcaggtcg gcgagcttcg acgcggtcgg cagctgcagc ccgagatact cggccaggtc
     2461 ggtgagcgag cagtcggggt acgtgccaag gcacgcgagc gcgcggaatt ccgagagcga
     2521 gagcccggcc ggccggctct gccgcgcctc ggcccgcacc agccgggtca cgaggagcga
     2581 cgtctcgaac acggcatccg ccattacttt cataagctaa gtattacgcg cccttgggcc
     2641 ggacgcaagc cgccgtcgcc ctccattacc ttccgaggcg ttcgcctgac cctatgcgcc
     2701 cttctctcgc cgtccccgcc ctcctgctcc tccccaccct gctcggcgcc cagaaccccc
     2761 tggcgcggcc gaccgacggg gtcgaggtgc gctacgtcac ctcccagccg gtggtgcagt
     2821 acgtcatcca cgtcgattcg accgacctgt cggggttcca ggtcgagctg cggctctcgg
     2881 ccctccccga caccttccgc ctggcgatgg cgcgccaccc ggagtacgac gaccggttct
     2941 ggcgctacct caaggggctg cgggtggaga cggcgggcgg ccccggcgtg gtcacccggc
     3001 tcgacgacgc gctctggcag gtcgtggcac ccggtggacg cgcgatcgtc cgctaccgca
     3061 tccagctgcc ggcgcctcag ccgctccgcg cggcgtggaa accgttcctc gcgcgggacg
     3121 gtggcctgat cggcggcacc cagagcctcc tgtacatcgt cggcgccacg ctcgcaccgg
     3181 cagtggtctc gctcgacctc ccggacgggt ggaacgccgc cacggggctc gttcccacgt
     3241 cggacgcccg gaccttcttt gcgccgacgg tggggatcct ggtcgactca ccgatcctga
     3301 tcgggaagtt cagcgaccgg cacttcttcg tcgacggcgt gccccaccgg atcgcctact
     3361 ggtcgctccc cgacgggacg ccattcgaca cggtcacctt ctcgcgcggg attgagagcg
     3421 tggtgcgcca ggcgatcgcg atcttcggcc gggcgccgta ccgcgaatac accttcctcc
     3481 tccaggacaa cgcctacggc gcactcgagc acctgaactc gctcacgctg ggcgcgccca
     3541 gcgccgagct tgcgaaggac ccgaccgggc tcctggagga gatcgcgcac gagtacttcc
     3601 acacctggga cctcctccgc atccggctgg caggagcggg cgacgtgacc tggcgcgccc
     3661 cgcgcccctc ctcggggctc tgggtgggcg aggggttcgc gatgtactac gccgacctcc
     3721 tgctccgccg ggccggcctc ccgatcgagg actccgcccg caccgatcac ctggcgcgcc
     3781 tcatggagca gtacctcgcc cgggaggacg gctacacgcg gtataccgcc gagcaggtga
     3841 gccaggtcgc gaacacggtc ccgggcaacc tcggcgacta cctcctcagt acccacctgc
     3901 aaggcgaatt gctcggcgcc atgctcgacc tccggatccg cgccgccacc cacgacgagc
     3961 attccctcga cgacgtgatg cgccggatga tggaacggtt ctccgccgac agcgggttct
     4021 ccacccacga cgtcgaggcg gtcgcgtccg aggtgtgcgg atgcaacctc aaggcgttct
     4081 tccgcgccta tgtgcgcggc cggaaaccga tcccgttcga ccagtacctg cgactgatcg
     4141 ggatgcactc ggaggtgagc tgggcgccgg cccggaacga cagcggagca gaacgtcctg
     4201 acctgcgggt cacggcgtgg atgccgcctg aagcgaaaca cccgagcctg atgctcttca
     4261 cgccgcaggg gatctggggt aaggcagggc tccacaccgg cgaccaggta ctgcggatcg
     4321 acggtgcgcc tatcgctgat gcggccgcct tccgcgccgc cctcggccgg ctcgcgatcg
     4381 gccagcccgc ccagcttgcc atcgagcggg acggcaagac catcggcatc cgggtggtga
     4441 tccgcgggta cgacgcgccg ggcgtcacca tcgttcccaa cgcccattcc accgccgacc
     4501 agcgcgcgct ccgaacggcg tggctctcag ggggccagta gatgctcttc ctgctcctcg
     4561 tcgcccaggc gccgtcacca cgttcgccgg cgcgggcggt ggcgctcgag gtcgagcatc
     4621 tccggacgcc gctcggcatc gacgcgacgc gcccccgctt ctcgtggcag atgtcggacc
     4681 cgcggcccgg cgcacggcag cgcgcgtacg agatcctcgt cgcgtcgtcg ccggagcgcc
     4741 tgacgccggc ccgcgccgac ctctggacga gcggcgtggt gcggagcagc gccaccctcg
     4801 acgtgccgta cgccggcacc gcgcttgcgc cgatgcgtcg ctactactgg accgtctgcc
     4861 tccgcgaccg cgaggaccgg cccgcgccct gcgcccccgc gtcctggttc gagaccggac
     4921 ggatggcgat gccgtggcag gcccggtgga tcgccgcgag ccgggcgatg ctcgacaccc
     4981 tcgcccacga cccgctcgag ggcgacaccg ccggcaccga cggccgcccg ccactcctgc
     5041 gcaaggacgt ggtcctcgcc gcccgccccg tccgggcccg catctacgcc accgccctcg
     5101 ggagctatcg gctcagcatc aacgggcggc cggtgagccg ggcggtgttc acccccgact
     5161 ggaccgacta ccgctatcgc gtcacctacc agacctacga cgtcacctcc ctgctccaca
     5221 ccggccccaa cgccctcggc gtcctcctgg gcgccgggtg gtacgccagc cgcttcgggt
     5281 tctcctccaa gcgctacgcc tatggcccgc cgccggtccg gttcctcctc gagctccacc
     5341 tgacgtacgc cgacgggcac actgaggtgg tgaccagcga cagcagctgg cagacggcgc
     5401 cgagcccggt gctgatctcg gagatctatg acggggaaac gtacgacgcg cgcctggagc
     5461 agccggggtg ggaccgcccc ggcttcgacg cggagtcgtg gcggcacgcc gtcgagctgc
     5521 cgccggtcaa ggtgaccatc cagccccagg cgatgccgcc gatcgaggag accgactccg
     5581 tccggccggt ccggcggtgg tcgcccgggg cgggcgtgta catcttcgac ctggggcaga
     5641 atatcgccgg gtgggcgcg
//
LOCUS       DASUAP010000345         3317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_856978, whole genome shotgun sequence.
ACCESSION   DASUAP010000345 DASUAP010000000
VERSION     DASUAP010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856978"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..336
                     /gene="rplW"
                     /locus_tag="VFU45_09340"
     CDS             58..336
                     /gene="rplW"
                     /locus_tag="VFU45_09340"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU5359312.1"
                     /translation="MHEIIVKPVVTEKSSASYQARKEYAFRVHPAATKPQIREALQKL
                     FGVTVTDVRTMQVRRREVNRGRTRGATAAWKKAIVTLKDGDSIAVFEG"
     gene            338..1177
                     /gene="rplB"
                     /locus_tag="VFU45_09345"
     CDS             338..1177
                     /gene="rplB"
                     /locus_tag="VFU45_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU5359313.1"
                     /translation="MSIKQFRPITAGTRFRMVSGFDEITRATPEKSLLEPVKKSGGRN
                     NLGHVTSRHIGGGHKRMFRKIDFKRNKFGVPAKVAEIEYDPNRSARIALLVYADGEKR
                     YILHPKGLSQGDTVVSGPGADVRLGNALPLQEIPLGTTVHNVELKIGKGGQMIRSAGS
                     SAQVVAKEGDYVTLRLRSSEMRRVNGRCLATIGEVGNSEHELISMGKAGKTRWLGQRS
                     KVRGVAKNPVDHPLGGGEGKTSGGRPPVSPWGKPEGRKTRRPKKASNQFVVRGRKRGK
                     ATQ"
     gene            1177..1467
                     /gene="rpsS"
                     /locus_tag="VFU45_09350"
     CDS             1177..1467
                     /gene="rpsS"
                     /locus_tag="VFU45_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU5359314.1"
                     /translation="MSRSIKKGPFVQESLLLKVQVLNSRNEKKVVKTWSRASTILPEF
                     VGHTFAVHNGNKFVPVYVTENMVGHKLGEFAPTRLFRGHGGKIVADKKAKPE"
     gene            1473..1829
                     /gene="rplV"
                     /locus_tag="VFU45_09355"
     CDS             1473..1829
                     /gene="rplV"
                     /locus_tag="VFU45_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU5359315.1"
                     /translation="MEAQAIQRMVRQSPRKMRLVIDEIRGKGVNEAYAILKFSKKLAA
                     RQIAKTLRSAVANAEQRALQSNEPFDVDELYVKKAVINMGQPLKRFTAAAQGRATPIR
                     KRTSHVELHVATRETK"
     gene            1829..2488
                     /gene="rpsC"
                     /locus_tag="VFU45_09360"
     CDS             1829..2488
                     /gene="rpsC"
                     /locus_tag="VFU45_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU5359316.1"
                     /translation="MGQKTHPIGFRLGVVKPWKSRWFATKDMPALLKEDELIRKYLKA
                     RLGHAAIAEVHVERRPGKVTVTIHTGRPGVVIGKRGAEVDKLRDELAQLTGKEAAVNV
                     EEIKRPELSAQLVGDNIAHQLVQRISFRRAMKRAVQSAMRMGAQGIRVRLSGRLGGAE
                     IARTEKYLEGRVPLHTLRADIDYATSTAKTTYGTIGIKVWIFKGEVVEELGGRTYSTG
                     N"
     gene            2492..2905
                     /gene="rplP"
                     /locus_tag="VFU45_09365"
     CDS             2492..2905
                     /gene="rplP"
                     /locus_tag="VFU45_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU5359317.1"
                     /translation="MLAPKRVKFRKQFKGRTKGTESRGTTVAFGEFALMALEPGWITN
                     RQIEAARVAMTREMKRGGKVWIRIFPDKPITKKPAETRMGKGKGNPEGWVAVVKPGRV
                     LFEIEGITEDLARKAMALAAAKLPVKTRFVKREEV"
     gene            2910..3119
                     /gene="rpmC"
                     /locus_tag="VFU45_09370"
     CDS             2910..3119
                     /gene="rpmC"
                     /locus_tag="VFU45_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU5359318.1"
                     /translation="MKTSELKELSADELRSKLEALRAERFNLRFRSATEAIDNPVQFR
                     TLRRDIARVLTALRAREGGAGGSEG"
     gene            3116..>3317
                     /gene="rpsQ"
                     /locus_tag="VFU45_09375"
     CDS             3116..>3317
                     /gene="rpsQ"
                     /locus_tag="VFU45_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU5359319.1"
                     /translation="MSQETTAAARARRKTRQGVVTSDKMARTVTVAIVRRFAHPIYGK
                     QVTRTTRTKARNDKGARLGDTVR"
BASE COUNT          642 a         1033 c         1243 g          399 t
ORIGIN      
        1 aagcccaggg ccgcgacgaa ggccccgaag aagaaggggg gcaggtagat gcccgcgatg
       61 cacgagatca tcgtcaagcc ggtggtgacc gagaagtctt cggccagcta ccaggcgcgg
      121 aaggaatacg cgttccgggt ccaccccgcg gccaccaagc cgcagatccg cgaagcgctg
      181 cagaagctgt tcggggtgac ggtcaccgat gtccgcacca tgcaggtgcg gcgccgggaa
      241 gtgaaccgcg ggcgcacccg cggcgcgacg gccgcctgga agaaggccat cgtgacgctc
      301 aaggacggcg attcgatcgc cgtattcgag ggctgacatg agcatcaagc aattccggcc
      361 gatcacggcg ggcacgcggt tccggatggt ctcggggttc gacgagatca cccgcgccac
      421 gcccgagaag tcgctgctcg agccggtgaa gaagtccggc gggcgcaaca acctcgggca
      481 cgtgacctcg cgccacatcg gcggcgggca caagcggatg ttccgcaaga tcgacttcaa
      541 gcggaacaag ttcggggtcc cggccaaggt ggccgagatc gagtatgacc cgaaccgctc
      601 ggcgcggatc gcgctcctgg tgtacgccga cggcgagaag cgctacatcc tgcacccgaa
      661 ggggctgagc cagggcgaca ccgtcgtctc cggtcccggc gccgacgtgc ggctgggcaa
      721 cgcgctcccg ctgcaggaga tcccgctggg caccacggtg cacaacgtgg agctcaagat
      781 cggcaagggc ggccagatga tccgctcggc cggctcgagc gcccaggtgg tggccaagga
      841 gggcgattac gtgacgctcc ggctccggtc ctcggagatg cggcgggtca acgggcgctg
      901 cctggcgacc atcggcgagg tggggaactc cgagcacgag ctgatctcca tgggcaaggc
      961 gggcaagacg cgctggctgg gccagcgctc gaaggtccgc ggcgtggcca agaacccggt
     1021 cgaccacccg ctgggcggcg gcgagggcaa gacgtcgggc gggcgcccgc cggtcagccc
     1081 gtggggcaag ccggaaggcc ggaagacccg gcgtccgaag aaggcgtcca accagttcgt
     1141 cgtgcgcggc cgcaagcgcg ggaaggcgac ccaataatgt cgcgtagcat caagaagggc
     1201 ccgttcgttc aggagtcgct gctcctgaag gtccaggtgc tcaacagcag gaacgagaag
     1261 aaggtcgtga agacctggag ccgggcgagc accatcctgc ccgaattcgt gggccacacg
     1321 ttcgcggtgc acaacggcaa caagttcgtg ccggtgtacg tgaccgagaa catggtcggc
     1381 cataagctgg gcgagttcgc gccgacgcgg ctgttccgcg ggcacggcgg gaagatcgtc
     1441 gccgacaaga aggccaagcc ggagtgagcg ccatggaagc ccaagcgatc cagcggatgg
     1501 tccgccagtc gccccggaag atgcggctcg tcatcgacga gatccgcggc aagggtgtga
     1561 acgaggcgta cgccatcctc aagttcagca agaagctcgc ggcccggcag atcgccaaga
     1621 cgctgcggtc ggcggtggcg aacgccgagc agcgggcgct ccagtcgaat gagcccttcg
     1681 acgtggacga gctctacgtg aagaaggcgg tcatcaacat gggccagccg ctcaagcgct
     1741 tcacggccgc cgcgcaggga cgggcgacgc cgatccggaa gcggaccagc cacgtggagc
     1801 tccacgtggc cacgcgggag acgaagtaat ggggcagaag acacatccga tcggcttccg
     1861 cctgggcgtg gtcaagccct ggaagtcgcg ctggttcgcc accaaggaca tgccggccct
     1921 cctgaaggag gacgagctga tccggaagta cctcaaggcg cggctggggc atgcggccat
     1981 cgccgaggtg cacgtggagc ggcggccggg gaaggtcacc gtgacgatcc acaccgggcg
     2041 gccgggcgtg gtgatcggca agcggggcgc cgaggtggac aagctgcgcg atgagctggc
     2101 gcagctgacg ggcaaggagg cggcggtcaa cgtcgaggag atcaagcggc cggagctctc
     2161 ggcccagctc gtcggcgaca acatcgcgca ccagctggtg cagcggatct cgttccgccg
     2221 ggccatgaag cgcgcggtcc agagcgcgat gcggatgggg gcgcagggga tccgcgtgcg
     2281 gctctcgggc cggctgggcg gggcggagat cgcgcggacg gagaagtacc tcgagggccg
     2341 ggtgccgctt catacgctgc gcgccgacat cgactacgcc acgagtacgg ccaagaccac
     2401 ctacggcacg atcgggatca aggtctggat cttcaagggc gaggtcgtcg aggaactcgg
     2461 cggccgcacc tacagcacgg ggaactgagc catgctggcg ccgaagcgag tcaagttccg
     2521 gaagcagttc aaggggcgca ccaagggcac cgagtcgcgc ggcacgaccg tggcgttcgg
     2581 cgagttcgcc ctgatggcgc tggagccggg gtggatcacc aaccggcaga tcgaggccgc
     2641 ccgcgtggcg atgacccgcg agatgaagcg cggcggcaag gtgtggatcc ggatcttccc
     2701 ggacaagccg atcaccaaga agccggccga gacgcgcatg ggcaagggca agggcaaccc
     2761 ggagggctgg gtggcggtgg tgaagccggg ccgggtgctg ttcgagatcg agggcatcac
     2821 ggaggacctg gcccgcaagg ccatggcgct cgcggcggcc aagctgccgg tgaagacgcg
     2881 cttcgtgaag cgcgaggagg tctgacgtgg tgaagaccag tgagctcaag gagctgtcgg
     2941 cggacgagct ccggagcaag ctcgaggcac tccgcgccga gcgattcaac ctgcgcttcc
     3001 ggtcggcgac cgaagcgatt gacaacccgg tgcagttccg cacgctgcgg cgcgacatcg
     3061 cccgggtgct gaccgcactc cgcgcccgcg agggcggggc gggagggagc gaaggatgag
     3121 ccaggagacg accgcggcgg cccgcgcgcg ccggaagacg cggcagggcg tcgtgaccag
     3181 cgacaagatg gcccggaccg tgacggtggc gatcgtgcgg cgcttcgcgc acccgatcta
     3241 cggcaagcag gtcacccgca ccaccaggac caaggcccgc aacgacaagg gggcccggct
     3301 gggtgacacg gtgcgga
//
LOCUS       DASUAP010000346         4832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_121406, whole genome shotgun sequence.
ACCESSION   DASUAP010000346 DASUAP010000000
VERSION     DASUAP010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121406"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFU45_09380"
     CDS             complement(<1..400)
                     /locus_tag="VFU45_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5359320.1"
                     /translation="MTVPLLEVTGLAKHFAEGGLLRRRPPVRAVDGVSFTIDAGETLA
                     LVGESGSGKSTVGRTLLRLEEPTAGAARFDGTDIFALDRRALREVRRKAQIVFQDPQS
                     SLDPRMTLGRSIAEGIEIHRLARGADVARRV"
     gene            complement(397..1371)
                     /locus_tag="VFU45_09385"
     CDS             complement(397..1371)
                     /locus_tag="VFU45_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5359321.1"
                     /translation="MTSLLSVYDLRLTFPGPGGPIHPVDGVSFSIARGETLALVGESG
                     CGKSLTALSLLRLVPTPGFIAAGSSIVFDGAEVLALAPGELRALRGRRVGMVFQDPLT
                     SLNPVMRIGDQIAEGIVAHGLASKAEARTRALALLREVGIADAEHRLDAYPHQLSGGM
                     RQRVVIAIALACEPELIIADEPTTALDVTVQAQILETLDRLRRDRGMALLLITHDFGI
                     VAGRADRVAVMYAGQVVEEGATPEVFAHPAHPYTQGLLASVPTMTGPVRRIEPIPGSV
                     PLPGSWPAGCRFAPRCARAFAACVAPAPVHPAGPGHSVRCWLGAEPER"
     gene            complement(1368..2168)
                     /locus_tag="VFU45_09390"
     CDS             complement(1368..2168)
                     /locus_tag="VFU45_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5359322.1"
                     /translation="MKRRAGIILLGAILLVAIAAPWALPAPDAQPDPLRLALLAPGLG
                     HWLGTDQLSRDVLARVVAGARVSLAIALLAVLVSVTLGAGAGLIAGYAGGATDVVLMR
                     LVDGALAIPRLFYLLLAAAAWDRMPLPAFILLLGLTGWFGTSRLVRAEVLRLRREGFV
                     QAAEAMGATRPRVILRHLLPNAAGPLLVAATLAVGDVILLEAGLSFLGLGVRPPTASW
                     GGMILEGKDVLARAPWVSLAPGIAIVITVLAVNLVGESIRTSLDPRTR"
     gene            complement(2165..3133)
                     /locus_tag="VFU45_09395"
     CDS             complement(2165..3133)
                     /locus_tag="VFU45_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5359323.1"
                     /translation="MSRWLAGRAAQAALTWAVALTILFFLMRLTPGDPLARLSADRPL
                     PPGAAEALRHLYGLDRPLLAQFVDFLRGALRGDLGVSIQFGLPVRTLIAQRLPATLLL
                     GGVVLLVDFTFGLWLGVHQATHQGGRIDRWLGNASLTAYAVPSFWTGLCLVWLGALKW
                     HAFPVAGMTDPLAGSGTGAWPHLLDTGRHLILPALTLVIATIAVTMRYQRAALLEALD
                     AEWIRTARAKGLPERAILRRHAWRAALGPVIALFGLWLPMLVTGAVFTEAVFAWPGLG
                     SLAADAVLARDYPLAMGCAALVAALVVLGNLVADVMQAALDPRVRR"
     gene            complement(3135..4763)
                     /locus_tag="VFU45_09400"
     CDS             complement(3135..4763)
                     /locus_tag="VFU45_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5359324.1"
                     /translation="MPTHSRRLLVLAAALVVAPALRAQSDHGSIVVVTGQYPGLPIPT
                     LVQGSSNQDVADLLFLRLARLGPTLATAGDSAFIPQLARRWTRRDSLTIVFELDPRAR
                     WQDGVPVTARDVVFSFARARDPGVSPALATLLRTIASVTALDERHVAIAFTRWYPSQF
                     YDATYQVQILPAHLLEGMAPAAVAASDFARAPVGDGPYRWVRGVPGEFVELGADPKFF
                     LGAPRLDRVIFRAAADGDARMNLLLSGEGDALENILPVANQDRVRADADLRLVAVPSF
                     TLGYFLYNERDPADTSRPHPILSDPGVRRALTLAIDRPAMARSVFGPAAEVPVGPASL
                     SSWIRVPEPAARQDTAAARRALEASGWTIRDRDGVAQREGRRLTLDVIFPNTSPARRQ
                     IALQAQAQWKALGVDAELEPLDFPTYLDRRNAGRFDIDFTAVNQDPNPTGLTQSWTCA
                     GGSNVAHYCDPKVDSLIAAASVSLDGLHLWQQALRAIDDDEAAAFVFAPAAMIAVHRR
                     YEHVVLHPESFWSGLGEWSVAPGRQLPRDRGAGR"
BASE COUNT          741 a         1650 c         1899 g          542 t
ORIGIN      
        1 acacccgccg cgccacgtcg gcgccccggg caagccggtg gatctcgatc ccctcggcga
       61 tggagcggcc gagcgtcatc cgcgggtcga gcgagctctg cggatcctgg aagacgatct
      121 gcgccttgcg gcgcacctcg cggagcgccc gccggtccag cgcgaagatg tcggtgccgt
      181 cgaagcgggc cgcgcccgcg gtcggctcct ccagacggag cagggtccgc cccaccgtgg
      241 acttcccgga gccggactcc cccaccagtg ccagcgtctc gccggcgtcg atggtaaagg
      301 acacgccgtc gaccgcccgg accgggggcc ggcgccgaag gagccccccc tccgcaaagt
      361 gcttggcgag accggtgacc tcgaggagcg gcaccgtcat cgttccggct ccgcgccgag
      421 ccagcagcgc accgagtgcc cggggccggc cgggtgcacc ggtgccggcg ccacgcacgc
      481 ggcgaacgcc ctggcgcacc ggggtgcgaa ccggcagccg gcgggccacg accccggcag
      541 cggaacgctc ccagggatcg gctcgatgcg gcggacgggt ccggtcatcg tcggcacgga
      601 cgcgagcagg ccctgcgtgt atggatgcgc cggatgggca aagacctccg gcgtggcgcc
      661 ttcctcgacg acctggccgg cgtacatcac tgccacccgg tcggcccggc ccgcgacgat
      721 cccgaagtcg tgggtgatga ggagcagggc catcccgcgg tcgcgacgca ggcggtcgag
      781 cgtctcgagg atctgggcct ggacggtcac gtcgagggcc gtcgtcggct cgtcggcgat
      841 gatcagctcc ggctcgcagg cgagcgcgat ggctatgacc acccgctggc gcatcccgcc
      901 gctcagctgg tgcggatagg cgtcgaggcg gtgctctgca tcggcgatgc cgacctcccg
      961 gagcagcgcg agcgcgcgcg tccgcgcctc ggccttcgag gcgaggccgt gggccacgat
     1021 cccctcggcg atctggtcgc cgatccgcat caccggattg aggctggtga gggggtcctg
     1081 gaagaccatc ccgacacgac gcccgcggag cgcgcggagt tcgccggggg caagggccag
     1141 cacctcggcg ccatcgaaga cgatggaact gccggccgcg atgaaccccg gcgtcggcac
     1201 caggcggagg agggagagcg ccgtgaggct cttgccgcag ccggactcgc cgacgagcgc
     1261 cagcgtctcg cccctggcga tcgagaacga cacgccgtcg accgggtgga tgggaccgcc
     1321 ggggccggga aaggtcaggc ggaggtcgta caccgacagc agcgaggtca tcgggtccgg
     1381 ggatcgaggg aggtgcggat cgactcgccg accaggttga cggccagcac ggtgatcacg
     1441 atcgcgatgc cgggggcgag gctcacccac ggcgcccggg ccaggacgtc cttgccttcg
     1501 aggatcatcc cgccccacga cgcggtcggc gggcggacgc cgaggccgag gaaggagagg
     1561 cccgcctcga gcaggatcac gtcgcccacg gcgagcgtcg cggcgacgag caggggaccg
     1621 gcggcgttgg ggagcaggtg ccgcaagata acccggggcc gggtggctcc catcgcctcc
     1681 gcggcctgga cgaagccctc ccgccgcaat cgcagcacct cggcccgcac cagccggctg
     1741 gtgccgaacc agccggtgag tccgagcagc aggatgaacg cggggagcgg catccggtcc
     1801 cacgccgccg cggcgaggag caggtagaac agccggggga tggcgagggc gccgtccacg
     1861 aggcgcatga ggacgacgtc ggtggcgccg cccgcgtagc cggcgatgag gcccgcaccg
     1921 gcgccgaggg tgaccgacac caggaccgcg aggagcgcga tggcgagcga cacccgggcg
     1981 cctgccacca cccgggccag cacatcgcgg ctcagctggt cggtgccgag ccagtggccg
     2041 agacccgggg cgaggagcgc caggcgcagc ggatccggct gggcatcggg cgccgggagc
     2101 gcccatgggg ccgcgatggc cacgagcagg atggccccga ggaggatgat gccggcgcgg
     2161 cgcttcaccg gcgcacccgg ggatcgagcg ccgcctgcat cacatcggcc acgaggttgc
     2221 cgaggacgac gagcgcggcg accagcgctg cgcatcccat cgcgagcggg tagtcgcgcg
     2281 ccaggacggc gtccgcggcg agcgagccca ggcccggcca cgcgaagacg gcctcggtga
     2341 agacggcacc ggtcaccagc atcgggagcc agagcccgaa gagcgcgatc accgggccga
     2401 gggccgcgcg ccaggcatga cggcggagga tggcccgctc ggggagtccc ttggcacggg
     2461 cggtccggat ccactcggcg tcgagcgcct cgaggagcgc agcccgctga tagcgcatgg
     2521 tgacggcgat cgtggcgatg accagcgtga gggcggggag gatgaggtgc cggccggtgt
     2581 cgagcaggtg gggccaggcc ccggtgccgc tcccggccag cggatcggtc ataccggcca
     2641 ccggaaaggc gtgccacttg agggcgccga gccagacgag gcagagcccg gtccagaatg
     2701 atggtaccgc gtacgccgtg agcgaagcgt tgccgagcca gcggtcgatc cggccaccct
     2761 ggtgcgtcgc ctggtgcacg ccgagccaca acccgaaggt gaagtccacc aggagcacca
     2821 cgccgcccag caggagcgtg gcgggaagcc gttgggcgat gagcgtccgc accggcaggc
     2881 cgaactggat cgacacaccg aggtcgcccc ggagcgcgcc gcggagaaag tcgacgaatt
     2941 gcgccagcag gggccggtcg agaccgtaga ggtgacggag ggcttccgcg gcgccggggg
     3001 ggaggggccg gtcggccgag agccgggcga ggggatcgcc cggggtgagt cgcatcagga
     3061 agaagaggat ggtgagcgcc acggcccacg tgagcgcggc ctgcgccgcg cgcccggcga
     3121 gccagcggct cacgtcaccg acccgcgccc cggtcacgtg gcagctggcg gcccggcgcc
     3181 accgaccatt cgccgagtcc gctccagaac gactccgggt ggagcaccac gtgttcgtag
     3241 cgccggtgca cggcgatcat cgccgcgggg gcgaacacga acgccgcggc ctcgtcgtcg
     3301 tcgatggcgc ggagtgcctg ctgccagagg tggaggccgt cgagggagac cgaggcggcg
     3361 gcgatgagcg agtccacctt cgggtcgcag tagtgcgcga cgttggagcc gccggcgcag
     3421 gtccacgact gggtgagacc ggtggggttc gggtcctggt tgaccgccgt gaagtcgatg
     3481 tcgaaccggc ccgcgttgcg gcggtcgagg taggtcggga agtcgagggg ctcgagttcg
     3541 gcgtccacgc ccagcgcctt ccactgggcc tgcgcctgga gggcgatctg gcgccgggcg
     3601 gggctcgtgt tcgggaagat cacgtcgagg gtgaggcgcc ggccctcgcg ctgggcgacg
     3661 ccgtcccggt cccgtatggt ccacccggac gcctccagcg cgcggcgggc cgcggcggtg
     3721 tcctgccgcg cggccggctc cggtacccgg atccacgacg acagcgaggc cggtccgacc
     3781 ggcacctccg ccgccggccc gaagacgctg cgcgccatcg cggggcggtc gatcgcgagc
     3841 gtcagggcgc ggcggacccc cgggtccgag aggatcgggt gcgggcgcga cgtgtccgcc
     3901 gggtcgcgct cgttataaag gaagtagccc agggtgaacg acggcaccgc caccaggcgg
     3961 aggtcggcat cggcgcgcac ccggtcctgg ttcgccaccg ggaggatgtt ctccagcgcg
     4021 tcgccctcgc cgctcaggag gaggttcatc cgcgcatcgc cgtcggcggc ggcgcggaag
     4081 atgacccggt cgagccgggg cgcgccgagg aagaatttcg ggtcggcccc gagttcgacg
     4141 aactcgcccg gcactcctcg gacccaccga tagggcccgt cgcccaccgg cgcgcgggcg
     4201 aagtcggatg cggccacggc cgcgggggcc atcccttcga gtaggtgcgc cgggaggatc
     4261 tggacctggt aggtcgcgtc gtagaactgg ctcgggtacc agcgggtgaa cgcgatggcg
     4321 acgtgtcgct catcgagcgc ggtgaccgat gcgatggtgc ggagcagcgt ggcgagggcg
     4381 gggctcacgc cggggtcgcg cgcgcgcgca aagctgaaga cgacgtcccg cgcggtcacc
     4441 ggcacgccgt cctgccagcg cgcccggggg tcgagctcga agacgatcgt cagcgagtcg
     4501 cggcgggtcc acctccgggc gagctgcggg atgaacgcgg agtcgccggc ggtggcgagt
     4561 gtggggccga gccgggcgag ccggaggaag agcaggtccg cgacatcctg gttgctgctc
     4621 ccctggacga gggtcgggat cggaagcccg ggatactgac ccgtcaccac gacgatgctg
     4681 ccgtgatcgg actgggcgcg gagcgccggt gcaacgacaa gcgcggcagc gaggaccagc
     4741 aggcggcggg agtgcgtcgg catgggctag aaggaatagc ggcgccgtgc cgacgcgcta
     4801 gggggtgctc agcgccgcca cgtccattcg tc
//
LOCUS       DASUAP010000347         3928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_679824, whole genome shotgun sequence.
ACCESSION   DASUAP010000347 DASUAP010000000
VERSION     DASUAP010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679824"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VFU45_09405"
     CDS             complement(<1..1022)
                     /locus_tag="VFU45_09405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU5359325.1"
                     /translation="MSRSLIPQRRIPWRHLVMFGWMPGPLKVLAYRLLLGYRIGRGVR
                     LSFGAAVIGDDVQLDDHVEIGVLAVVQGRRIRIGRHSSVGTMSYVSCNLIEIGEDARI
                     REQVFVGGPQLPESRFTLGSRTIILQSTNINPTKPVTIGDDTGIGGHCLIFTHGAWLN
                     VLDGYPATYEPVTIGSSVWLPWRVFLMPGAVIGDGSVIGANSLVSGTIPARSLAVGTP
                     AKVIRSAPQFPPAVDAQARAAKVAEILSEFDRYVRDCGTTIDPGSPRTYHRDGRSWRM
                     HWRHAGTPLGHIGAGETLLTEEPLLPDETAALRRAGAHWLDLAGRTRSEGGSPLTEEL
                     AAFIGR"
     gene            1137..2552
                     /locus_tag="VFU45_09410"
     CDS             1137..2552
                     /locus_tag="VFU45_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359326.1"
                     /translation="MIRLPYDGLAPAGERGLRLLVDASRVVPAAPSVAGAVVLEVVPG
                     GGPDPMAGPEAFAVVPGIVRAPLGVLERVAAWAGATEEEGSPARDRFDRVPSGVNPFV
                     AAGAERRPVLSELAAGFRRAVAAAAGDGPFRAVAPWPGGHRWAAVLSHDLDVVAGWPL
                     FTALRVLELGRKGDVGRSLRVLGAAAVAGRDPITAGVADLLHIEAELGVRSTWFILCG
                     DPTLRSWRRGDLTYRPESRRASGILARIRGAGHELGVHGSFDTIERPELLATQRARLA
                     ALDGTPPAGIRQHFLRMRPMETQRAMARAGFQYDSTFGFADRNGFRLGLADVVPLEGA
                     ADFDLLPFCWMDRALSKYQEIEDPAAWIDDGLALARMCRQVDGVWCGIWHPNLVPALG
                     FPGGLAAYRALVSGIQASGDPWIAPAEAVVEWRRARRSVAIAGVDASGKVLATGTRSA
                     LGPLRLEDQHGNAREPVEAAR"
     gene            2549..3718
                     /locus_tag="VFU45_09415"
     CDS             2549..3718
                     /locus_tag="VFU45_09415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5359327.1"
                     /translation="MRILFVSHTYLRWMGDRAGAQVWRLAVAAQEAGHTVLVVAPHAP
                     GAAIDEVLGGVEVRRFRYAPDTLERIGYQGAVQKSAGSPIALALMPWYLARFRRAVRQ
                     CVRGFHPDVIHAHWWIPAAIAAVGQGVPFVVTGHGSDVRLLGRSVALRALAHQVLRRA
                     AAVTAISGIMAEDICRWARIPVVQVTPSAVDETRFHPMPQAPVDPPRILFAGNWIRAK
                     GVDLILEAFAQIRRRGISARLRLVGDGPGQAEFRALADRLGVAPDVEWAGTRAHEEMP
                     GEFAAAAVVVMASRGPRGEGLPITLVEAMMSGCAVVATPAGGIPEIVIDGDTGLIARD
                     GDASHLADQIARLLLDPALRDRLASAGRARATGRFARGPAFAKVLGILAQAASPP"
     gene            3715..>3928
                     /locus_tag="VFU45_09420"
     CDS             3715..>3928
                     /locus_tag="VFU45_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359328.1"
                     /translation="MNLRRFLRPLGVAVALAVAALAIRALVGAWRQAASTPVQWAFSP
                     GWLAAALAVALLTYLVLVESWRRTLHG"
BASE COUNT          486 a         1383 c         1466 g          593 t
ORIGIN      
        1 tagcgcccga tgaacgcggc gagctcctcg gtgagcgggg agccgccttc gctccgggtc
       61 cggccggcca ggtcgagcca gtgcgcgccc gcgcggcgga gcgcggcggt ctcgtcgggg
      121 aggagcggct cctcggtcaa cagcgtctct cctgcgccga tgtggccgag cggcgtgccc
      181 gcatgccgcc agtgcatccg ccacgagcgg ccgtcgcggt ggtacgtccg gggcgagccc
      241 gggtcgatcg tggtgccgca gtcgcgcacg tagcggtcga actccgacag gatctcggcc
      301 actttcgccg cgcgggcctg ggcatccacg gcggggggga attgcggcgc cgagcggatg
      361 accttggccg gcgtcccgac ggcgaggctg cgggccggga tggtgccgct caccagcgaa
      421 ttggcgccga tcacgctccc gtcgccgatc acggcaccgg gcatcaggaa cacccgccac
      481 gggagccaga cgctggagcc gatggtcacc ggctcgtagg tggccgggta cccgtcgagc
      541 acattgagcc aggcgccgtg ggtgaaaatg aggcagtgcc cgccgatgcc ggtgtcatcg
      601 ccgatggtca ccggcttggt cgggttgatg ttggtcgatt gcaggatgat cgtgcggctg
      661 ccgagggtga atcgggattc cgggagctgc ggcccgccca cgaacacctg ctcccggatc
      721 cgcgcatcct cgccgatctc gatcaggttg cacgagacat agctcatcgt gccgacgctc
      781 gaatggcgcc cgatccggat ccgccgaccc tggaccacgg ccaggacgcc gatctccacg
      841 tggtcgtcga gctggacatc atcgccgatg acggcggcgc cgaacgacaa ccggaccccc
      901 cggccgatgc ggtagcccag gagcaggcga taggccagga ccttgagcgg gcccggcatc
      961 catccgaaca tcaccaggtg ccgccacgga atgcggcgct ggggtatcag ggagcgtgac
     1021 atggcgacgc gatcgagcgc gcaggtgtgg ggaggacggc cggagacgag cgggcgccca
     1081 agggtagtcc gcgcggcggc ctcgcgtcaa gcgccaccgt actcccggca gggcccatga
     1141 tccgccttcc ttacgatgga ctcgccccgg caggggagcg cggccttcgg ctcctcgtcg
     1201 atgcgtcgcg cgtcgtcccg gccgcgcctt cggtcgcggg tgcggttgta ctggaggtcg
     1261 tgcctggcgg gggacccgac ccgatggccg gaccggaggc gttcgccgtg gtgccgggca
     1321 tcgtgcgggc gccgctcggg gtgcttgagc gggtggcggc gtgggccggc gcgacggagg
     1381 aagagggctc gccggcacgg gaccggttcg accgggtccc gtcgggcgtc aacccgttcg
     1441 tcgccgccgg ggccgagcgg cgtcctgtgc tgtcggagct ggcggcggga ttccggcgtg
     1501 ccgtggccgc ggccgcgggc gacggcccat tccgcgcggt ggcgccatgg cccggcggcc
     1561 accgctgggc ggcggtcctg tcgcacgacc ttgacgtcgt ggccggatgg ccgctcttca
     1621 cggcgctccg ggtgctcgaa ctcggccgga aaggcgacgt cggccggtcg ctccgcgtgc
     1681 tcggggcggc cgcggtggcc ggacgcgatc ccatcaccgc cggcgtggcg gacctgctgc
     1741 acatcgaggc ggaactcggg gtccggagca cctggttcat cctctgtggc gacccgaccc
     1801 ttcgctcgtg gcgccgcggc gacctgacct accggccgga gtcccggcgc gccagcggca
     1861 tcctggcccg gatccgtggc gccggccacg agctcggcgt gcacggaagc tttgacacga
     1921 tcgagcgccc cgaactgctc gccacccagc gggcccgcct tgccgcgctg gacggcacgc
     1981 cgcccgccgg gatccgccag cacttcctcc ggatgcgtcc gatggagacc cagcgcgcga
     2041 tggcccgcgc gggcttccag tacgactcga cgttcggctt tgccgatcgc aacgggttcc
     2101 ggctcggcct tgccgacgtg gtcccgctgg aaggcgcggc cgatttcgat ctcctgccat
     2161 tctgctggat ggaccgggcg ctcagcaagt accaggagat cgaggacccg gcggcctgga
     2221 tcgatgacgg cctggccctg gcccgcatgt gccgtcaggt cgacggcgtg tggtgcggca
     2281 tctggcaccc caacctcgtg ccggcccttg gattccccgg cgggctggcg gcgtaccgcg
     2341 ccctggtgtc cggcatccag gcgtccggcg atccgtggat cgccccggcg gaggcggtcg
     2401 tggaatggcg ccgcgccagg cggtcggtgg ccatcgccgg cgtcgatgcc agcggcaagg
     2461 tcctcgccac cggaactcgc tctgccctcg ggccactccg gctggaagac cagcacggca
     2521 atgcccggga gccggtcgag gcagctcgat gaggatcctg ttcgtcagcc atacctacct
     2581 tcggtggatg ggcgaccgcg cgggcgccca ggtatggcgc cttgccgtcg cggcgcagga
     2641 ggctggccac acggtcctgg tggtggcgcc ccacgccccg ggtgcggcca tcgacgaggt
     2701 gctcggtggg gtggaggtcc ggcgcttccg ctatgccccc gatacgctcg aacggatcgg
     2761 ttatcagggc gccgtccaga agagcgcggg gtcgcccatc gcgctcgcgc tgatgccgtg
     2821 gtacctggcg cgcttccgac gggctgtccg ccagtgcgtg cgcggcttcc atcccgacgt
     2881 gatccacgcg cactggtgga tcccggccgc gatcgccgcg gtggggcagg gcgtcccgtt
     2941 cgtggtgacc gggcacggca gcgatgtgcg gttattgggg cgctctgtcg cattgcgtgc
     3001 gctggcgcac caggtgctgc gccgcgcagc ggccgtgacc gcgatctccg gaatcatggc
     3061 ggaggacatc tgccggtggg cccggatccc ggtggtgcag gtcaccccct ccgcggtcga
     3121 cgagacccgc tttcacccca tgccgcaagc gccagtggat ccgccgcgga tcctgttcgc
     3181 cgggaattgg atccgtgcca agggggtcga cctcatcctc gaggcctttg cccagatccg
     3241 gcgccgtggc atctccgctc gcctgcgcct ggtcggtgac ggccctggac aggcggaatt
     3301 ccgggcgctg gccgaccggc tcggagtcgc gcccgacgtg gaatgggcgg gcacccgggc
     3361 tcatgaggag atgcccggcg agttcgccgc cgctgcggtc gtcgtgatgg cgagccgagg
     3421 cccgcgcggc gaggggctgc cgatcacgct ggtggaggcc atgatgtcgg gctgcgccgt
     3481 cgtcgccacg cccgccggcg gcatccccga gatcgtgatc gatggagaca cgggattgat
     3541 cgcgcgggac ggcgatgcgt cgcacctggc cgatcagatc gcccgtctgc tgctggatcc
     3601 cgcgcttcgt gatcgcctcg cttcggcggg ccgggcgcga gcgaccggcc ggttcgcccg
     3661 gggcccggcc ttcgcgaaag tgctcgggat cctggcccag gccgcgagcc ccccgtgaac
     3721 ctccgacggt tcctccgccc gctcggcgtg gccgtcgcac tcgccgtcgc cgcccttgcc
     3781 atccgcgcac tggtgggtgc gtggcgacag gcggccagca cacccgtcca atgggcgttc
     3841 agccctggct ggctggccgc ggccctcgcg gtggcgctgc tcacttacct ggtgctggtc
     3901 gagagctggc gccgcacgct ccacggct
//
LOCUS       DASUAP010000348         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_734544, whole genome shotgun sequence.
ACCESSION   DASUAP010000348 DASUAP010000000
VERSION     DASUAP010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734544"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VFU45_09425"
     CDS             <1..910
                     /locus_tag="VFU45_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5359329.1"
                     /translation="FPADLPESETIRLRLTVHDQTAGILTLHDTVPIRLTPPKRRFLE
                     TLAYYAALAAERVPLMREAEHAAALREAARFKDIVLASVSHDLRTPLAAIKAIAGDPR
                     LQEGERSRHIEEQIDRLSRLVGDLLDLTRLQTGTLRIEAEANTAEDLIGALDRQLTAA
                     LGGRELSLSVDLAQPALVGKFDFVKSLRALSNLAENAIRLSPSDQPVELSVEREGEFL
                     AFRVADRGPGVAEADRARIFEAFFRPEGATPDAGRAGLGLSIAQRMAEMQGGSVSYAP
                     RPGGGSVFTLRLPALDLEEPAEEGEA"
     gene            928..1086
                     /locus_tag="VFU45_09430"
     CDS             928..1086
                     /locus_tag="VFU45_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359330.1"
                     /translation="MRRKNGNGERRRRGKGAGERKRRRSRREEKEQERRRREEKERGE
                     GVKRRSEE"
     gene            complement(1176..1961)
                     /locus_tag="VFU45_09435"
     CDS             complement(1176..1961)
                     /locus_tag="VFU45_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359331.1"
                     /translation="MTRAPGAGAVGLLLAAALHAQAPDPHAVQPERPTVATHAYTVAT
                     GWVEIEAGAEFDRYPDKSRGGSAPVVVKVGAGRRAQLDLFGSAAEPPGQGLGIGDVGV
                     ALKWRAVERSRWLGDLSLQPSLKLPTGEASAGRGTGTTDLGLVVISSRQVGSVELDLN
                     VGYVHRSGDGSAAPRDATLWTASFGGPLRGAVGWVAECYGYPATSGPAGAASVVAVLA
                     GPTLLVRKWLAFDAGVILPVTGPQPHAVYVGTVYNVGRAWGGE"
     gene            complement(1958..3304)
                     /locus_tag="VFU45_09440"
     CDS             complement(1958..3304)
                     /locus_tag="VFU45_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU5359332.1"
                     /translation="MNTSATPPVEELAGWRAPRRGPSLPEVHGSIRIPAGAGWLRKLL
                     AFAGPGYLVAVGYMDPGNWATDLAGGARFGYALLSVIFLSNLMAILLQSLALRLGVAT
                     GRDLAQACRDHYPAPVRWGLWLLAEAAIAACDLAEVIGAAIALNLLFHIPLLWGVCLT
                     ALDVLLILLLQQRGFRWLEAFVIALVVTIAVVFAIELLLARPAWHEVAAGLVPAAGIV
                     TDPAMLYIAVGILGATVMPHNLYLHSAIVQTRQVGPDEASRREAIHFGSIDSAIALGF
                     AFFINAAILVLAAATFHHSGHAGVAEIGTAYRLLSPLLGTALASTLFAVALLASGQNS
                     TITGTLAGQIVMEGFLDIRLRPWLRRLLTRGIAIIPAIVVTALQGERGTADLLILSQV
                     ILSLQLPFAVIPLVQFTGDRRKMGAFVSGPAMQVAAWMTAGAIVALNAWLLIGMLR"
     gene            complement(3301..>3864)
                     /locus_tag="VFU45_09445"
     CDS             complement(3301..>3864)
                     /locus_tag="VFU45_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU5359333.1"
                     /translation="APSVTGMCKRLDEQGYVRHEPYRGVTLTAHGRRAALRTLRHHRL
                     LEAYLVAFLGYDWDDVHAEAERLEHAVSPELIERMARALGNPSVDPHGDPIPAPDGTI
                     VEVIYTPLTELAAGTRAEVKRVDSNDADRLRYLASVGLKPGAEVRVTERHPFRGPVTV
                     ETQVGLQVVGRELGALVLCLPRPGGAG"
BASE COUNT          658 a         1355 c         1378 g          473 t
ORIGIN      
        1 cttccccgcc gacctccccg aatccgagac gatccggctt cgcctcacgg tgcacgacca
       61 gaccgcgggc atcctgacgc tgcacgatac cgtacccatc cggctcaccc cgccgaagcg
      121 tcgtttcctc gagacgctgg cctactacgc ggccctcgcg gccgagcggg tcccgctgat
      181 gcgggaggcg gagcacgcgg cggccctccg cgaggcggcc cggttcaagg acatcgtcct
      241 cgcatcggtg tcgcacgacc tccggacgcc gctcgcggcc atcaaggcga tcgccggcga
      301 cccccgcctg caggagggcg agcgctcccg gcatatcgag gagcagatcg accggctgag
      361 ccggctcgtc ggggacctcc tcgacctgac ccggctccag accggcaccc tccgcatcga
      421 ggccgaggcc aacaccgccg aggacctgat cggcgccctc gaccgccagc tcaccgccgc
      481 gctcggcgga cgcgagttgt cactgtcggt ggatctggca caaccggcgc tggtagggaa
      541 gttcgacttt gtgaagtcgc tgcgcgccct cagcaacctg gccgagaacg ccatccgcct
      601 gagcccttcg gatcagccgg tggaattgag cgtcgaacgc gagggggagt tccttgcctt
      661 ccgcgtggcg gaccgcggac ccggcgtcgc ggaagcggac cgcgcgcgga tcttcgaggc
      721 gttcttccgt cccgaaggcg cgaccccgga cgccggtcgc gccggcctgg gcttgagcat
      781 cgcacagcgg atggcggaga tgcagggcgg gagcgtgagc tatgcccccc gccccggcgg
      841 cggcagcgtc ttcacgctcc gactccccgc actcgacctc gaggaaccgg cggaggaagg
      901 ggaagcgtaa gaagtaagaa gtaagaagtg agaaggaaga atggaaatgg agagaggaga
      961 aggagaggaa aaggagcagg agagaggaaa aggagaagga gcaggagaga ggaaaaggag
     1021 caggagagga gaaggagaga ggagaaggag agaggagaag gagtgaagag aaggagtgaa
     1081 gagtgaaaag tgaacagtga agagtgaaca ggggcgccgg tgggtcacgg tagccccttc
     1141 ttacttctca cttcttactt ttcccttttc actctttact ccccacccca cgcccggccc
     1201 acgttgtaca ccgtccccac atacaccgcg tgcggctgcg ggccggtgac gggcaggatg
     1261 acgccggcgt cgaacgccag ccatttccgc accagcagcg tcggtcccgc cagcaccgcc
     1321 accaccgacg ctgcgccggc aggcccgctg gtcgcgggat acccgtaaca ctccgccacc
     1381 caaccgaccg cgccgcggag cggcccgccg aaggacgcgg tccagagcgt cgcgtcgcgc
     1441 ggcgcggcgg agccgtcccc cgaccggtgg acatagccga cgttgaggtc cagctcgacc
     1501 gatcccacct gccggctcga aatgacgacg agcccgaggt ccgtggtgcc cgtgccccgc
     1561 cccgccgatg cctcgccggt ggggagcttg aggcttggct gcagcgacag gtcgccgagc
     1621 cagcgtgagc gctcgacggc gcgccacttg agcgccacgc cgacatcacc gatcccaagc
     1681 ccctgccccg gcggctcggc ggcggacccg aagaggtcga gctgagcccg gcggcccgcc
     1741 cccaccttga cgaccaccgg cgccgagccg ccccgcgact tgtcggggta acggtcgaat
     1801 tccgcccctg cttcgatctc cacccagccg gtggcgacgg tgtacgcatg ggtggccacg
     1861 gtggggcgct ccggctgcac cgcgtggggg tcgggcgcct gcgcgtggag cgccgccgca
     1921 aggaggagtc ccaccgctcc ggcgcccggg gcccgcgtca tcggagcatg ccgatgagca
     1981 gccaggcatt gagcgcaacg atggcgccgg cggtcatcca cgcggcgacc tgcatcgccg
     2041 ggccgctcac gaacgcgccc atcttgcgcc ggtcgccggt gaactgcacc agcgggatga
     2101 ccgcgaacgg gagctggagg ctcaggatca cctggctcag gatgagcagg tcggcggtcc
     2161 cgcgttcgcc ttgcagggcc gtcacgacga tcgccgggat gattgcgatc ccccgcgtga
     2221 gcagccggcg gagccagggc cgaagccgga tgtcaaggaa tccctccatc acgatctggc
     2281 cggcgagggt cccggtgatg gtcgaattct ggcccgacgc caggagcgcc accgcgaaga
     2341 gcgtgctggc gagcgccgtc cccaggagcg gcgagagcag ccggtacgcc gtgccgatct
     2401 cggccacgcc ggcatgcccc gaatggtgga aggtggcggc ggcgaggacg aggatcgcgg
     2461 cgttgatgaa gaaggcgaag ccgagcgcga tggcggagtc gatcgagccg aagtggatgg
     2521 cctcccggcg agaggcctcg tcggggccga cctggcgggt ctggacgatc gcggaatgca
     2581 ggtagaggtt gtgcggcatc accgtggcgc cgaggatccc gacggcgatg tagagcatcg
     2641 cggggtcggt cacgatcccc gcggccggga cgagccccgc cgcgacctcg tgccaggccg
     2701 gccgcgccag caggagctca atggcgaaca ccaccgcgat cgtgaccacc agcgcgatga
     2761 cgaacgcctc cagccaccgg aaaccgcgct gctgcagcag gaggatcagc agcacgtcga
     2821 gcgcggtgag gcacacaccc cacaggagcg ggatgtggaa gagcaggttg agcgcgatgg
     2881 ccgcgccgat cacctcggcc aggtcgcacg ccgcgatggc cgcctcggcc aggagccaga
     2941 gcccccaccg caccggcgcc gggtaatggt cgcggcacgc ctgggccagg tcccgccccg
     3001 tcgccacgcc gagccggagg gccagcgact gcagcaggat cgccatcagg ttcgagagga
     3061 agatcaccga gaggagcgcg tagccgaagc gcgccccacc cgccaggtcg gtggcccagt
     3121 tgccgggatc catgtagccg acggccacga ggtacccggg gcccgcaaag gcgagcagct
     3181 tccggagcca ccccgcgccc gccgggatgc ggatggaccc atgtacctcc ggcaggctcg
     3241 gcccccggcg gggggcgcgc caaccggcca gctcctcgac cggcggcgtg gcggaggtgt
     3301 tcaccctgcc cccccgggac gcggcaggca gagcaccagc gcgccgagct cgcgcccgac
     3361 gacctgcaga ccgacctgcg tctccacggt caccggcccg cggaacgggt ggcgctcggt
     3421 cacccgcacc tcggcgcccg gcttgagccc caccgacgcc aggtaacgga gccgatcggc
     3481 gtcattcgag tcgacccgct tcacttccgc gcgcgtgccg gcggcgagct cggtgagcgg
     3541 ggtgtagatc acctcgacga tcgtgccgtc gggggcgggg atcgggtcgc cgtgcggatc
     3601 gacgctcggg ttccccaggg cccgcgccat ccgttcgatc agctcgggcg agacggcgtg
     3661 ctccaggcgc tccgcctcgg cgtgcacgtc gtcccagtcg tagccgagga acgcgaccag
     3721 gtacgcctcg agcaggcggt ggtgccggag ggtgcggagg gcggctcggc gtccgtgcgc
     3781 cgtgagcgtg accccacggt acggctcgtg gcggacgtac ccctgctcgt cgagccgctt
     3841 gcacatcccc gtcaccgacg gcgc
//
LOCUS       DASUAP010000349         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_298018, whole genome shotgun sequence.
ACCESSION   DASUAP010000349 DASUAP010000000
VERSION     DASUAP010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298018"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFU45_09450"
     CDS             complement(<1..598)
                     /locus_tag="VFU45_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5359334.1"
                     /translation="MIYVCVPCHNEGETVGLLLWKVRQVFSDFPREYQLLVGDDASTD
                     QTADVLGPYAKVLPLTVVRHRTRQGYARTVEALLRLATERSDRPKRDYAVILHADFSH
                     SPAILPEIVKRLDSGADVVVAEGHLTPAGEPSRGYRLLRRWGRLLVPRAARVPGVHDP
                     ISGCMAFRLIALTSAMRNWPDALLRAEGWAANAELIAKA"
     gene            complement(664..2556)
                     /locus_tag="VFU45_09455"
     CDS             complement(664..2556)
                     /locus_tag="VFU45_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5359335.1"
                     /translation="MSDALRTVAEFARELAGTAEARTGVARLAARLHAAFAPDACAVA
                     LTRGDYEAGAPLHAEPPPGERYRNLLTLALRRGSQHLSAAEAEGRLGLAAGDAPAHDL
                     LIVPITTASDRLGALLLGGTAARWTAADVALAEALAAQAGAALAAIERIPAAEAWERV
                     ADALSIALCIVDGRGRVKGANRSFARLMRSTPAELPGWPWLSLVPPAWAMSLRELFDE
                     SNDGRREAELKASGRTLLATAFPLPGGTSGEHVLVLDDQTERRRLQDQLVQSEKLSAI
                     GQLIAGVAHELNNPLTSVVGFADFLAEAPDVPAGLREPLSVIQQEATRASTLVRNLLR
                     FARRHEPERRRQPLRPILEGTLALLRAQFVASGIEIHVTGEPGIPDVNVDAPRVQQIF
                     LNLINNAAQAIGTTGRTGTITIRLRPWLDGAAVDVRDDGPGMTPEVAAQAFEPFFSTK
                     PEGQGTGLGLAISQGIAKEHGGRISLTTAPGAGATFTVEFPGDGRPAAEEPAEPAVAA
                     AGPRRILVIDDEPHILHYMRATLESWGHSVDTADDGARGLERARATEPDLIITDLRMP
                     KMSGREFFESLAVVRPDLAARVAFSTGDTIRGDTLAFLERQGRPVLHKPFSLAELRRL
                     IAVAAA"
     gene            complement(2553..>3049)
                     /locus_tag="VFU45_09460"
     CDS             complement(2553..>3049)
                     /locus_tag="VFU45_09460"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU5359336.1"
                     /translation="GELEPDRDAFEFAMLDAARARGLPVLAICRGMQVVNVALGGTLW
                     QDLPTERRGSVQHDGTWARADRVHAVEIARGSTLASAVGATSIAVNSFHHQGIRALAP
                     GLLPTATAPDGLVEGIEGADGQWLVGVQWHPEAFHAEPDSPDARLFRTFVQACALRPA
                     GTTA"
BASE COUNT          401 a         1048 c         1183 g          417 t
ORIGIN      
        1 ccgccttcgc gatcagctcg gcattggccg cccagccctc ggcgcggagc agggcgtccg
       61 gccagttgcg catggcactt gtgagcgcga tgagccggaa cgccatgcat ccggagatcg
      121 ggtcatgcac gccggggaca cgggctgcac gggggacgag caggcgtccc cagcgacgga
      181 gcagtcggta cccgcgcgac ggttcgcccg ccggggtcag gtgcccttcg gcgacgacga
      241 cgtcggcccc gctgtcgagg cgcttgacga tctccgggag gatcgcgggg ctgtgggaga
      301 agtcggcgtg gaggatgacc gcgtagtcgc gcttcgggcg gtcggagcgc tcggtggcca
      361 gccgcagcag cgcctcgacc gtcctggcgt agccctggcg ggtccggtga cgcaccacgg
      421 tgagcggaag gaccttggcg tacggcccca gcacatcggc ggtctggtcg gtcgaggcgt
      481 cgtcgcccac gaggagctgg tactcccggg ggaagtcgct gaagacctgc cggaccttcc
      541 agaggaggag gccgaccgtc tcaccttcgt tatggcacgg cacgcacacg tagatcatcg
      601 cgattccggc tggtgtcccg gggaacgata caaggtggac ggtcgggcgg ccaacccggc
      661 tcctcaggcc gccgcgacgg cgatgagccg gcggagctcc gcgaggctga acggcttgtg
      721 caggacgggg cgaccctggc gctcgagaaa ggcgagggta tcgccgcgga tcgtgtcacc
      781 ggtgctgaag gccacccggg ccgccaggtc gggccggacc acggccagcg actcgaagaa
      841 ctcccgcccg ctcatcttcg gcatccggag gtcggtgatg atgaggtcgg gctcggtggc
      901 ccgggcgcgt tcgagcccgc gcgccccgtc gtccgcggtg tcgacgctgt ggccccacga
      961 ctccagggtc gcgcgcatgt aatggaggat gtggggctcg tcgtcgatga cgaggatgcg
     1021 gcgcgggccc gcggcggcca ccgccggttc ggcgggttcc tccgcggcgg ggcgcccgtc
     1081 gccggggaac tcgacggtga acgtggcgcc ggcgcccggc gccgtggtga gcgagatcct
     1141 gccgccgtgt tccttcgcga tgccctggct gatggcgagg ccgagccccg tgccctggcc
     1201 ctcgggcttg gtgctgaaga acggctcgaa tgcctgtgcg gcgacctcgg gggtcattcc
     1261 ggggccgtcg tcgcggacgt cgaccgcggc cccgtcgagc cagggccgga gccggatggt
     1321 gatcgtgccg gtccgacccg tggtgccgat ggcctgcgcg gcgttgttga tgaggttgag
     1381 gaagatctgc tggacccggg gcgcatcgac gttgacgtcc gggatgcccg gctcgccggt
     1441 gacgtggatc tcgatgccgc tcgcgacgaa ctgggcgcgc aggagcgcca gcgtcccttc
     1501 gaggatcggg cgcaggggct ggcgccggcg ctccggctcg tggcgccggg caaagcggag
     1561 caggttccgg accagcgtcg atgcccgggt cgcctcctgc tggatgaccg acagcggctc
     1621 gcggaggccg gcaggcacgt cgggtgcctc ggccaggaag tcggcgaacc cgacgacgga
     1681 ggtgagcggg ttgttgagtt cgtgcgccac gccggcgatg agctggccga tggccgagag
     1741 cttttccgac tggaccagct gatcctgcag gcggcggcgt tcggtctggt catcgagcac
     1801 caggacgtgc tcgccgctgg tgccgccggg gagcgggaac gcggtggcga gcagggtccg
     1861 gcccgaggcc ttgagctcgg cctcgcgacg cccgtcattc gactcatcga acagctcccg
     1921 caggctcatc gcccaggccg gcggcaccag gctcagccag ggccacccgg ggagctctgc
     1981 cggggtgctc cgcatcaggc gcgcgaagga ccggttggcg cccttgaccc ggccccgccc
     2041 gtccacgatg cagagcgcga tcgacagggc gtcggccacc cgttcccacg cctcggcggc
     2101 cgggatccgc tcgatcgcgg cgagcgccgc cccggcctgg gcggcgagcg cctcggcgag
     2161 ggccacgtcg gcggccgtcc agcgcgcggc tgtgccaccg aggaggaggg cgccgagccg
     2221 gtcgctcgcc gtcgtgatcg ggacgatgag caggtcatgc gccggtgcgt cgcccgcggc
     2281 cagaccgagg cgtccctccg cctcggcggc ggagaggtgc tggctgccgc gccgcagtgc
     2341 cagggtcagg agattgcggt agcgctctcc cggcggcggt tcggcgtgga gcggcgcgcc
     2401 ggcctcgtag tcgccgcggg tgagtgccac cgcgcaggcg tccggggcga acgccgcgtg
     2461 gaggcgggcg gcgagccgcg ccacccccgt gcgcgcctcg gccgtgcccg cgagctcgcg
     2521 cgcgaattcg gcgaccgtcc ggagcgcgtc gctcacgcgg tcgtcccggc gggtcggagt
     2581 gcgcaggcct gcacgaatgt ccggaacagg cgggcgtccg gcgagtcggg ctcggcgtga
     2641 aaggcctcgg gatgccactg cacccccacc agccattgcc catccgctcc ctcgatcccc
     2701 tcgacgaggc catccggtgc agtggccgtc gggaggaggc caggcgccaa tgcccggatc
     2761 ccctggtgat ggaacgaatt gaccgcgatc gaggtcgcgc cgacggccga cgcgagcgtg
     2821 ctgccgcgag cgatctccac ggcgtggacc cgatcggcgc gggcccacgt cccgtcgtgc
     2881 tgcacgctgc cgcgccgctc ggtcggaagg tcctgccaga gggtaccgcc gagggcgacg
     2941 ttcaccacct gcatcccgcg gcagatcgcg agcaccggca gtccgcgggc gcgggcggcg
     3001 tcgagcatcg cgaattcgaa ggcatcccgg tcgggctcga gttcaccga
//
LOCUS       DASUAP010000350         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_952942, whole genome shotgun sequence.
ACCESSION   DASUAP010000350 DASUAP010000000
VERSION     DASUAP010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952942"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFU45_09465"
     CDS             complement(<1..610)
                     /locus_tag="VFU45_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5359337.1"
                     /translation="MHGRSDAPAVAGTALLATYAQMPVRPVRGEGSWLIEADGTRWLD
                     AYGGHAVASTGHCHPKVVRAIQEQAARLVFYSTAVPLPQRELLADALVARMPAPLSKV
                     FFCNSGAEANENALHLARKWTGRSKVVSVTGGWHGRTVATLAVTDGEHYQSFAKRAGM
                     PLSIKVPFDDVAAMHATVDADTAAVIVEPVQGMGGARDASVAF"
     gene            complement(613..1626)
                     /gene="argC"
                     /locus_tag="VFU45_09470"
     CDS             complement(613..1626)
                     /gene="argC"
                     /locus_tag="VFU45_09470"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU5359338.1"
                     /translation="MKVAVIGAAGDAGGELLRILLGHPQVTDIVATSRSQAGKPLAEV
                     HPQLATLTDRKFLAASAAEAAGGRDVVFLALEHGESSKVAGAVLEAAPGLVIDLAADF
                     RTADTALYERFYGPHPSPELLPRFTYALADVAGTALRGARALAAPGCFATAAQLALYP
                     LRQAGVSPSLFAITGSSGAGVSPRPTTHHPARANNVFAYSVLGHRHEAEILERWREWG
                     ASGAEPRLMTHSGPFVRGIYLTLHARSEQGGLSAELYSRAFAGRPFVRILAQAPELTH
                     CVGTNYALMHAASNAAGTEIQVMVAIDNLVKGAGGQAIQAMNLSLGLPETAGLEFGGI
                     FPC"
     gene            complement(1623..2705)
                     /locus_tag="VFU45_09475"
     CDS             complement(1623..2705)
                     /locus_tag="VFU45_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359339.1"
                     /translation="MVVTTRVRLDRIASSTKNARLGPDVIVGNEIVAAEGYVLAVRIL
                     EDKPTYNTLEDLAGRMVSLRAGDVLAGTLGARRALRGYAGEVPGHIATGDVINVLNLG
                     GILGRCTSVNPEIGQPFKAEVLGAILAFPELGDRVGRPAHIRDGAVPTADRLQLDVPV
                     VYVAGTCMNAGKTVAATELVRGLSRAGLRVAAAKLTGVSLMRDSLSMRDAGAVAALTF
                     NDAGAASTHAGMTVGVAKGIFNRLAGERPDVIVAELGDGILGEYGVQDILQDRELAAA
                     GAAYVMAAPDPVGCWGAVELMRERFGLPVAVLTGPATDNEVGRDYIRASLGIPAFNAR
                     RDADGLVNVVQEALARWPAPAAAVSA"
     gene            complement(2707..>3669)
                     /locus_tag="VFU45_09480"
     CDS             complement(2707..>3669)
                     /locus_tag="VFU45_09480"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEU5359340.1"
                     /translation="FLIQGNVLRGEVYPLSVAAERTQQAIAVVDVARSIGAAAVAHGS
                     TGAGNDQIRFDVAFRVLAPDLEIVTPIREQNLQRADAIADLKRRGLPVPGQAGAYSIN
                     RGLWGTTWGGGWTHDPWAGPPAELLDPPAGAPAPREIVLAWRAGLPVALDGAGAEGPE
                     LVDRINLLARAYGFGRDIHVGETTIGIKGRVGFEAGAALLLIRAHRELEKLVLTKWQA
                     FWKDQLARFYGDRLHEGQYFDPALRDIEALLTSSQARVEGETRVRLAPGRFQVVGARS
                     PYSMMDPAVATYGEDNRLWTGDEARGFARVSAIPSLIARRAGED"
BASE COUNT          500 a         1369 c         1323 g          477 t
ORIGIN      
        1 ggaacgcgac cgaggcgtcc cgcgccccgc ccatcccctg caccggctcg acgatcacgg
       61 ccgccgtgtc ggcgtcgacg gtcgcgtgca tcgcggccac gtcgtcgaac ggcaccttga
      121 tcgagagcgg catcccggcc cgcttcgcga aggactgata gtgctcgccg tcggtcaccg
      181 cgagcgtcgc gacggtgcgg ccgtgccagc cacccgtgac ggagacgacc ttcgaccgtc
      241 cggtccactt ccgcgccagg tggagggcgt tctcgttggc ctccgcgccc gaattgcaga
      301 agaacacctt gctgaggggc gccggcatcc gggccaccag cgcgtccgcc agtaactcgc
      361 gctgcgggag tggcacggcc gtcgaataga agacgaggcg cgcggcctgc tcctggatgg
      421 cgcgcacgac tttcgggtgg caatgcccgg tcgaggcgac ggcgtgcccg ccgtaggcgt
      481 cgagccagcg ggtgccatcg gcctcgatga gccagcttcc ctccccccgc accggccgca
      541 ccggcatctg ggcgtaggtg gccagcagtg cggtgccggc caccgccgga gcgtcggagc
      601 gtccgtgcac ggtcaacaag ggaagatccc cccgaactcg agccccgccg tctccgggag
      661 gccgagtgaa aggttcatgg cctggatcgc ctggccgccg gcgcccttga ccaggttgtc
      721 gatggccacc atcacctgga tctcggtgcc ggccgcgttc gacgccgcgt gcatcagggc
      781 gtagttggtg ccgacgcagt gcgtgagctc cggcgcctgc gccaggatcc gcacgaacgg
      841 ccgcccggcg aacgcccggg agtagagctc ggcggagagc ccgccctgct cgctccgtgc
      901 gtggagcgtg agatagatgc cgcgcacgaa ggggcccgaa tgcgtcatca gcctcggctc
      961 cgccccgctc gcgccccact cccgccaccg ctcgaggatc tccgcctcgt gccggtgccc
     1021 gagcacgctg tacgcgaaca cgttgttcgc ccgcgcgggg tgatgggtgg tgggccgcgg
     1081 cgatacgccg gcgccgctcg accccgtgat ggcaaagagc gacggcgaga cacccgcctg
     1141 ccggagcgga tagagcgcca gctgcgccgc ggtcgcgaag cagccgggtg cggcgagcgc
     1201 gcgcgcgccc cggagcgccg tcccggcgac gtcggcgagc gcatacgtga atcgcggcag
     1261 cagctccggc gacgggtgcg ggccgtagaa ccgctcatag agcgcggtgt cggcggtgcg
     1321 gaagtcggcg gcgaggtcga tcacgagccc gggggcggcc tcgagcaccg ccccggccac
     1381 cttcgacgac tcgccgtgct ccagcgccag gaagacgacg tcccgtccgc ccgccgcctc
     1441 cgccgccgag gccgcgagga acttccggtc ggtgagcgtc gcgagctgcg gatgcacctc
     1501 ggccagcggc ttcccggcct ggctccggct ggtggccacg atgtcggtca cctgcgggtg
     1561 cccgagcagg atgcggagga gttcgccgcc ggcgtcgccg gcggcgccga tgacggcgac
     1621 cttcacgccg acaccgcggc ggcgggcgcg ggccagcggg ccagcgcctc ctggaccacg
     1681 ttcacgagcc cgtcggcgtc acgccgcgcg ttgaatgcgg ggatgccgag ggaggcccgg
     1741 atgtagtcgc ggccgacctc gttgtcggtg gcggggccgg tgagcaccgc gaccgggagc
     1801 ccgaaccgct cccgcatcag ctccaccgcg ccccagcacc cgaccggatc gggtgccgcc
     1861 atcacgtacg ccgcgccggc ggccgccagc tcgcgatcct gcaggatgtc ctgcacccca
     1921 tactcgccca ggatgccgtc tcccagctcg gccacgatca cgtcgggccg ctcgccggcg
     1981 aggcgattga agatcccctt cgccacgccg acggtcatcc cggcgtgggt gctcgccgcc
     2041 ccggcgtcgt tgaaggtgag cgccgccacg gcccccgcat cgcgcatgga gagggagtcg
     2101 cgcatcagcg agacgccggt cagcttggcg gccgccaccc ggagccctgc gcgcgacagc
     2161 ccgcgcacga gctcggtggc cgcgacggtt ttccccgcgt tcatgcaggt gccggccacg
     2221 tacaccaccg gcacgtcgag ctgcagccgg tcggcggtgg ggacggcccc gtcgcggatg
     2281 tgcgcgggac ggcccacccg gtcgcccagc tcgggaaagg cgaggatggc gccgagcacc
     2341 tcggccttga acggctggcc gatctcgggg ttgaccgagg tgcagcggcc gaggatcccg
     2401 cccaggttga ggacgttgat cacgtcgccg gtggcgatgt gacctggcac ctcgccggcg
     2461 tagccccgga gcgcccggcg ggcgccgagg gtgccggcca ggacgtcgcc ggcgcggagc
     2521 gacaccatcc gccccgccag gtcctcgagc gtgttgtagg tcggcttgtc ctccaggatc
     2581 cgcaccgcga ggacgtaccc ctcggcagcc acgatctcat tgccgacgat cacgtcgggg
     2641 ccgagccggg cgttcttggt ggacgaggcg atgcggtcga gccggacgcg cgtcgtgacg
     2701 accatgtcag tcctcccccg cccggcgcgc gatgagcgag gggatcgccg agacccgcgc
     2761 gaatccccgc gcctcgtcac cggtccagag ccggttgtcc tcgccgtagg tggccacggc
     2821 ggggtccatc atcgaatagg ggctgcgcgc ccccaccacc tggaagcgcc ccggcgccag
     2881 gcgcacccgc gtctccccct cgacccgcgc ctgcgagctc gtgaggagcg cctcgatgtc
     2941 gcggagcgcc ggatcgaagt actgcccctc gtgcaggcgg tcgccgtaga agcgcgccag
     3001 ctggtccttc cagaacgcct gccacttggt gagcacgagc ttctcgagtt cccggtgggc
     3061 gcggatcagg aggagcgccg cgccggcctc gaagccgacc cgccccttga tgccgatcgt
     3121 cgtctcgccg acgtggatgt cgcgcccgaa tccgtaggcg cgggccagca ggttgatgcg
     3181 gtcgaccagc tccgggccct ccgcgccggc gccatcgagc gccaccggga ggccggcacg
     3241 ccaggccagg acgatctccc gcggcgcggg cgcccccgcc ggcgggtcga gcagctccgc
     3301 gggcggcccc gcccacggat cgtgggtcca gccgccgccc caggtggtgc cccagagccc
     3361 acggttgatg gagtaggcgc ccgcctgccc gggtaccggg agtccccgcc gcttgagatc
     3421 ggcgatggca tcggcgcgct ggaggttctg ctcgcggatc ggggtgacga tctcgagatc
     3481 cggcgccagg acccggaagg ccacgtcgaa acggatctgg tcgttgccgg caccggtgga
     3541 cccgtgggcc accgccgccg cgccgatcga gcgggccacg tccaccaccg cgatggcctg
     3601 ctgggtccgc tccgccgcca cgctcagcgg gtacacctcc ccccggagca cgttgccctg
     3661 gatgaggaa
//
LOCUS       DASUAP010000351         6856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_353214, whole genome shotgun sequence.
ACCESSION   DASUAP010000351 DASUAP010000000
VERSION     DASUAP010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353214"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="VFU45_09485"
     CDS             complement(<1..1820)
                     /locus_tag="VFU45_09485"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU5359341.1"
                     /translation="MQRIETEFAGRPLKIETGRLAKQAAGSALVQFGETVVLAAVTVS
                     PNQSSLPFFPLTVEYREKTYAAGKIPGGFLKREGRPSDKEILAARGIDRSIRPLFPEG
                     FKNEVQVFVTVLSADQQNDADILGVTAASIALSRSVIPWNGPIAGVRVGKVEGNWILN
                     PTFQQLEFSTIDLTVCGSADSINMVEGGSLEVSEAEMLEALKVAQKGLRDVLAITEKA
                     TKGKVEKLAWKKDEADPALAKRVKELAEKAIAQAINGKEKATRYGQVKKVKADVAAKL
                     AEEFPEAGRMVGHLLEEIEYAALRAQVLDKGERVDGRDTDTIRPIEITPAFLPRTHGS
                     AIFQRGETQALVSVTLGTQDDEQRIDSIDVAGETTKSFMLHYNFPPYCTGEVKPMRGT
                     SRREIGHGALAERALQPLLPAYDQFPYTLRVVSEILESNGSSSMATVCGGSLALMDAG
                     VPMRAPCAGVAMGLIKEGKKVAILTDILGAEDHLGDMDFKVAGTEAGITSIQMDIKIE
                     GLDLKVIAEALEKARKGRLHILGEMKKALPTHRADLSPHAPRIITIQIKTDKIGDVIG
                     PKGKTIRGIQDTTGAKISIEDSGLVTIAAVGGEAGQKAKE"
     gene            complement(1908..2168)
                     /gene="rpsO"
                     /locus_tag="VFU45_09490"
     CDS             complement(1908..2168)
                     /gene="rpsO"
                     /locus_tag="VFU45_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU5359342.1"
                     /translation="MTYDKQGVVTKHRRHDKDTGSAAVQVALLTERINSLGGHFEQHR
                     KDHHGRRGLLKMVGQRRRLLTYLERTDLATYRALVVQLGLRH"
     gene            2266..3210
                     /locus_tag="VFU45_09495"
     CDS             2266..3210
                     /locus_tag="VFU45_09495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5359343.1"
                     /translation="MPPADSPSPGTQLGRHTLVRLMGQGGFAAVWEARRDDGKSFAAK
                     ILKPRYSGDENFELRFRQESQFAANLLHPNIIHIEEVGRQGFWVYFTMDLYPASLATL
                     LAQGAPVEEPRLIAIAGDVAEALRFAHEQGVVHRDVKPDNILLGADGRAVVTDFGIAR
                     AVSGYVSATGVNMTIGTPHYLSPEQAQGRPLDGRSDIYSFGITLYRAATGDLPFKSRD
                     WYELARMHVEEPPEAPRARRPELSRRLERIILRCLAKHPDDRYATAADLAADLGAIHS
                     KDRSTETFQVPTPYLRAVSDTPKPANPDASSKKWWKPW"
     gene            complement(3430..4776)
                     /gene="rseP"
                     /locus_tag="VFU45_09500"
     CDS             complement(3430..4776)
                     /gene="rseP"
                     /locus_tag="VFU45_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU5359344.1"
                     /translation="MSTLLGLLVVLGILVFIHEAGHFAAAKWAGIWVHRFSLGLGTPI
                     KALSFTRGGTEYCISWLPLGGYVKMASREEEATTSALEGGAVTSPVPPGGYYEDKPVW
                     KRMIVTLAGVGMNTLFAWSAFVFLAAKNGKQVIPETRIGRVDTVALPAGAEALARLTP
                     GDRVTAVDGKAVHSWDDVTAGLISAQGSTVSLTVNDSLSVVLRIPPAALERRADAAQA
                     LAPWQPAVVELVQPSWPAARAGVAPGDTIVAVDGRPIVQWYDLVDVLKANPGRKLAVE
                     VGRAAGRTTLHVTPEPFDTAGKTIGRVGIGPHIDVVSTRYSLASAVGAGTAATAQSAL
                     TIVRTIQGLATRKISAKNLGGPIMIGQIAAQSAKLGLDAFLGVLGLISINLAMLNLLP
                     IPVLDGGQAVFLLYEGVVRRPMPVRWREALMLVGIALVVLLMLLANWNDVRRIFGW"
     gene            complement(4773..5912)
                     /gene="dxr"
                     /locus_tag="VFU45_09505"
     CDS             complement(4773..5912)
                     /gene="dxr"
                     /locus_tag="VFU45_09505"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU5359345.1"
                     /translation="MRGDLSRAPRGIALLGSTGSIGESTLNVLRRQRDHFRLVALTGG
                     RNRERLEAQVAEWRPAFAGLVHPGAVNGLPSGPECLIEAATRPDVDIVVNAVVGAAGL
                     DATLAALAAGKRVALANKETLVMAGQLVLEAARRGGGEIVPVDSEHSAVLQCVAGEAR
                     TLSRLILTASGGPLREWPAERLATASVEDALRHPTWRMGAKISVDSATLANKALEVIE
                     AHFLFGLGYDALEVVVHPQSVVHAFCEFEDGSVLAQLGFPSMELPILYALTHPTRLQD
                     SGVRRFDPVAAGPLTFEPVRTDAFRAFAAGIAAGRAGGTAPAVFNAANEVAVAAFLAG
                     EIPFGRIAESIERVLEAHAGQPATDLAAVRAADRWARDLTRKSLS"
     gene            complement(5912..6769)
                     /locus_tag="VFU45_09510"
     CDS             complement(5912..6769)
                     /locus_tag="VFU45_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HEU5359346.1"
                     /translation="MASNMTRRVAFAAVAIPLAGAVLWWGGWPLVVLVAAAGVLGTRE
                     LYDLARQMGVAPLAGLGLTAAALLPPFAFLATGPAPSASVSAVDAAVLGLLVLLAVAL
                     WRRAPGEHPLPAVAVTFFGVLYTGALPCYLIVIRHGRFGVQSWGGLALVMFPLVVTWT
                     CDTAAMFGGKFVGGPKLAPVISPGKTWAGAVAGVLGAVVVAPLYLRWALGPAGIVLSA
                     AQVVAFAAVLAVVGQLGDLAESLFKREAGVKDSSHLIPGHGGVLDRFDSLYFVLPAAA
                     FIYRALGVL"
BASE COUNT         1048 a         2419 c         2367 g         1022 t
ORIGIN      
        1 atctccttgg ccttctggcc cgcctcgccg cccaccgcgg cgatggtgac gagcccggag
       61 tcctcgatcg agatcttggc gccggtggtg tcctggatgc cccggatggt cttgcccttc
      121 ggcccgatca cgtcgccgat cttgtcggtc ttgatctgga tcgtgatgat ccgcggtgcg
      181 tgcgggctca ggtcggcgcg gtgcgtcggc agcgccttct tcatctcccc gaggatgtgg
      241 agccggccct tgcgggcctt ctccagcgcc tcggcgatga ccttgaggtc aagcccctcg
      301 atcttaatgt ccatctggat cgacgtgatg cccgcctcgg tgcccgccac cttgaagtcc
      361 atgtcgccca ggtggtcctc ggcgcccagg atgtcggtca ggatggcgac cttcttgcct
      421 tccttgatga gccccatcgc gacgccggcg cacggcgccc gcatcggcac gcccgcgtcc
      481 atcagcgcca gcgagccgcc gcacacggtg gccatcgagc tcgagccgtt cgactcgagg
      541 atctccgaca ccacgcgcag cgtgtagggg aactggtcgt acgccgggag cagcggctgg
      601 agcgcgcgct ccgccagcgc cccgtgcccg atctcgcggc ggctggtgcc gcgcatcggc
      661 ttgacctcgc cggtgcagta gggcgggaag ttgtagtgca gcatgaacga cttggtggtc
      721 tcccccgcca cgtcgatgct gtcgatgcgc tgctcgtcgt cctgggtgcc gagggtcacg
      781 ctgaccagcg cctgggtctc gccgcgctgg aagatggccg atccgtgcgt gcggggcagg
      841 aaggccgggg tgatctcgat gggacggatg gtgtcggtgt cccggccgtc gacccgctcg
      901 cccttgtcga gcacctgcgc gcggagcgcc gcgtactcga tctcctcgag caggtggccg
      961 accatccggc ccgcctccgg gaactcctcg gccagcttgg cggccacgtc ggccttgacc
     1021 ttcttcacct ggccgtagcg ggtggccttc tccttgccgt tgatcgcctg ggcgatcgcc
     1081 ttctcggcca gctccttgac gcgcttggcc agcgccgggt cggcctcgtc cttcttccac
     1141 gccagcttct cgaccttgcc cttggtggcc ttctcggtga tggccagcac gtcgcggagg
     1201 cccttctgcg ccaccttgag tgcctccagc atctccgcct cggagacctc gagcgagccc
     1261 ccctcgacca tgttgatcga gtcggcgctg ccgcagaccg tcaggtcgat ggtggagaac
     1321 tcgagctgct ggaaggtggg gttgaggatc cagttgccct cgaccttgcc cacgcgcacc
     1381 ccggcgatcg gcccgttcca cgggatgacc gaacggctga gcgcgatcga cgccgccgtg
     1441 acgccgagga tgtcggcgtc gttctgctgg tcggcggaga ggaccgtcac gaagacctgc
     1501 acctcgttct tgaacccctc ggggaagagc ggccggatcg accggtcgat gccccgcgcg
     1561 gccaggatct ccttgtcgga gggacggcct tcacgcttga ggaacccgcc ggggatcttg
     1621 cccgccgcgt aagtcttctc gcggtactcg acggtcaggg ggaagaacgg gagggacgac
     1681 tggttggggg agacggtcac cgccgccagg accacggtct cgccgaactg gaccagcgcg
     1741 gacccggcag cctgcttcgc cagacggccg gtctcgatct tgagggggcg cccggcgaat
     1801 tctgtctcga tacgttgcac gttcacgacc tttggggtac ggagcacatc aatggcagaa
     1861 gggcgtccca tgtgctcgta tgggacgccc ctgccgcgct acgcgagtta gtgacggaga
     1921 ccgagctgga ccaccaacgc ccgatacgtg gcgaggtcgg tccgctcgag gtaggtcagc
     1981 aggcgccggc gctgcccgac catcttgagc aggccccggc gaccgtggtg gtccttccgg
     2041 tgctgctcga agtggccccc gagggaattg atccgctcgg tgagaagcgc tacctgcacg
     2101 gccgccgaac cggtatcttt gtcgtggcgg cggtgcttgg tgacaacgcc ctgcttgtcg
     2161 tacgtcatgc tcggtctgtc ctgtccgtgg cgcggtcctg atcgattatt gattcgttcg
     2221 tagcatcgaa aagtaataac cccacaacag cttaggcaac ccccggtgcc gcctgcggac
     2281 tccccctccc ctggtaccca gctcggtcgc cacaccctgg tccgcctcat gggccagggc
     2341 ggattcgcgg cggtctggga agcccggcga gacgatggta agtcctttgc cgccaagatc
     2401 ttaaagccca ggtactcggg cgacgagaat ttcgagctcc gcttccgcca agaatcccag
     2461 ttcgccgcca acctgctcca ccccaacatc atccacatcg aagaggtggg gaggcagggg
     2521 ttctgggtct acttcacgat ggacctctac cccgcctccc tggccaccct cctggcccag
     2581 ggcgccccgg tcgaggagcc ccggctcatc gcgatcgccg gggatgtggc cgaggcgctc
     2641 cgcttcgccc acgaacaggg cgtggtccac cgcgacgtca agccggacaa catcctcctc
     2701 ggcgccgacg gccgggcggt cgtgaccgac ttcgggatcg cccgggcggt gagcggatac
     2761 gtctccgcca ccggcgtcaa catgacgatc ggcaccccgc actacctgag cccggaacag
     2821 gcgcaggggc ggcccctcga cgggcggtcg gacatctact ccttcgggat cacgctctac
     2881 cgcgccgcca ccggcgacct ccccttcaag tcgcgcgact ggtacgagct cgcccggatg
     2941 catgtggagg agccccccga agccccccgc gcccggcgac cggaactctc ccgccgcctg
     3001 gagcggatca tcctgcgctg cctggccaag caccccgacg accgctatgc caccgccgca
     3061 gaccttgccg ccgacctcgg cgccatccac tcgaaggacc gttcgaccga gaccttccag
     3121 gtgccgacgc cctacctccg cgccgtgagc gacaccccca aacccgccaa cccggacgcc
     3181 agctcgaaga aatggtggaa gccgtggtga gcgagatcgt gagaggtgag acgtgagagg
     3241 caagaggtga tgggtgagtg ggaagaggga aaagggaaaa gggaatcgag gggacgctcg
     3301 tgaacaacgt gacggggtga gcagaacaat gcgaggggga ttccgaggca accgggatcc
     3361 cccttttccc ttttcccttt tccctattcc cgcttcgcct gctccctgtc acgtctcacg
     3421 tcttacgtct caccaaccaa agatccgccg cacgtcgttc cagttggcga ggagcatcag
     3481 cagcaccacc agggcgatcc ccaccagcat cagcgcctcc cgccaccgga ccggcattgg
     3541 acggcggacg accccttcgt agaggaggaa cacggcctgg ccgccgtcga ggacggggat
     3601 cggcaggagg ttgagcatcg cgaggttgat cgagatcagg ccgagcacgc cgaggaaggc
     3661 gtcgagcccg agcttggcgc tctgcgccgc gatctgtccg atcatgatgg ggccacccag
     3721 gttcttggcg gagatcttcc gggtggcgag cccctggatg gtgcggacga tcgtgagcgc
     3781 ggactgggcg gtggcggcgg tgccggcccc gaccgcgctc gcgagcgagt agcgggtgga
     3841 caccacgtcg atgtgcggcc cgatccccac ccgcccgatc gtctttccgg cggtgtcgaa
     3901 cggttccggc gtcacgtgga gggtggtccg gccggcggcg cgccctacct cgaccgcgag
     3961 cttgcggccc gggttcgcct tgagcacgtc caccaggtcg taccattgca cgatcggccg
     4021 accgtccacc gccacgatcg tgtcgcccgg cgccacgccg gcgcgggccg cgggccacga
     4081 cggctgcacc agctcgacca cggcgggctg ccacggtgcg agtgcctgtg cggcgtccgc
     4141 ccggcgttcg agcgcggcgg gggggatccg gagcaccacg ctcaggctgt cgttcaccgt
     4201 gagcgacacc gtgctcccct gcgcggaaat gaggccggcg gtcacgtcgt cccacgaatg
     4261 gaccgccttc ccgtccaccg ccgtgacccg gtcccccggg gtgagccgcg ccaatgcctc
     4321 ggcaccggca gggagcgcca cggtgtccac ccgcccgatc cgcgtttcgg gaatgacctg
     4381 cttcccgttc ttggccgcga ggaacacgaa ggccgaccag gcgaagagcg tgttcatccc
     4441 gacgccggcc agcgtcacga tcatccgctt ccacaccggc ttgtcctcgt agtacccgcc
     4501 gggcggcacc gggctcgtga cggcgccccc ctcgagcgcg ctggtggtgg cctcctcttc
     4561 ccggctggcc atcttgacgt agccgccgag ggggagccac gagatgcagt actcggtgcc
     4621 accccgggtg aaactgagcg ccttgatcgg agtgccgagg cccagcgaga agcggtgcac
     4681 ccagatgccg gcccatttgg cggcggcgaa gtgccccgcc tcgtggatga acaccaggat
     4741 gccgaggacg acgagcaggc cgaggagggt gctcacgaca gggactttcg ggtaaggtcg
     4801 cgcgcccagc ggtcggcggc gcgcacggcg gcgaggtcgg tggccggctg gcccgcgtgg
     4861 gcctcgagca cccgctcgat cgactccgcg atgcggccga aggggatctc gccggccagg
     4921 aaggcggcga ccgccacctc atttgcggcg ttgaacactg ccggggccgt gcctccggcc
     4981 cgccccgcgg cgatcccggc ggcaaaggcc cggaaggcgt cggtgcgcac cggctcgaac
     5041 gtgaggggac cggccgccac cgggtcgaac cgccgcaccc cgctgtcctg gagccgcgtc
     5101 gggtgggtga gggcgtacag gatggggagc tccatcgacg ggaagccgag ctgcgccagc
     5161 acgctcccgt cctcgaactc gcagaaggcg tgcaccacgc tctggggatg gaccaccacc
     5221 tcgagcgcgt cgtagcccag gccgaacagg aaatgcgcct cgatgacctc gagtgccttg
     5281 ttggcgagcg tggcgctgtc gacgctgatc ttggccccca tccgccaggt cgggtgccgg
     5341 agcgcgtcct ccaccgatgc ggtggccagt cgctccgcgg gccactcccg gagcgggccg
     5401 cccgaggcgg tgaggatgag ccggctgagc gtgcgggcct cgccggcgac gcactgcagc
     5461 accgcgctgt gctcagagtc gaccgggacg atctcgccgc cacccctccg cgccgcctcg
     5521 agcaccagct gcccggccat gaccagcgtc tccttatttg ccagggcgac ccgcttcccc
     5581 gccgcgagcg ccgccagggt cgcgtcgagc cccgccgctc ccacgaccgc gttgacgacg
     5641 atgtcgacgt cgggccgggt ggcggcctcg atgaggcact cgggcccgct ggggaggccg
     5701 ttgacggcac cgggatggac caggccggcg aaggccgggc gccattccgc cacctgcgcc
     5761 tcgagccgct cccggttcct gccgccggtg agcgctacca ggcggaagtg gtcccgctgg
     5821 cggcgcagga cgttgagggt gctttcgccg atggagccgg tggagcccag gagggcgatc
     5881 cctcgcggtg ctcgggacag gtcgccgcgc atcagaggac gccgagggcg cggtagatga
     5941 acgccgccgc gggaaggacg aagtagagcg agtcgaagcg gtcgagcacg cccccgtgac
     6001 cggggatcag gtggctcgag tccttgacgc ccgcctcgcg cttgaagagc gactcggcca
     6061 ggtcgccgag ctgtcccacg accgcgagca cggccgcgaa ggcgaccacc tgggcggcgg
     6121 acaggacgat ccccgccggc ccgagcgccc atcgcaggta gagcggtgcc acgaccacgg
     6181 cgcccaggac cccggccact gcgccggccc aggtcttccc ggggctgatg accggcgcaa
     6241 gcttgggccc gccgacgaac ttgccgccga acatcgccgc ggtgtcgcag gtccaggtga
     6301 cgacgagggg gaacatcacc agcgcgagcc cgccccacga ctggacgccg aagcgtccgt
     6361 gccggatgac gatcaggtag caggggagcg cgccggtgta gagcacgccg aagaaggtca
     6421 cggccaccgc gggcagcggg tgctcgcccg gcgcacggcg ccagagcgcc acggccagga
     6481 gcacgaggag cccgagcacc gcggcgtcga cagcggacac ggaggccgaa ggtgcgggcc
     6541 cggtggcaag gaaggcgaac ggagggagga gggccgcggc ggtgagcccg aggcccgcga
     6601 ggggcgccac gcccatctgc cgggccaggt cgtagagctc acgggtgccg aggacgccgg
     6661 cggccgccac caggacgacg agcggccagc cgccccacca gagcacggca cccgccagcg
     6721 ggatggccac ggcggcgaac gccacccggc gcgtcatgtt gctcgccatc gtcagaccga
     6781 gacgcgaccg aagcgccgct cccgccgctg ataatcgagg atggcccgga agaggtccgt
     6841 ccgggtgaag tcgggc
//
LOCUS       DASUAP010000352         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_393644, whole genome shotgun sequence.
ACCESSION   DASUAP010000352 DASUAP010000000
VERSION     DASUAP010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393644"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..907
                     /locus_tag="VFU45_09515"
     CDS             128..907
                     /locus_tag="VFU45_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEU5359347.1"
                     /translation="MPTYSEYLKINELLELQRPLSDGPEHDEMLFIVIHQVYELWFKQ
                     VLHEIGHLESLLGRNDGHRAQHTLKRILTILKVMVGQLDVLETMTPLEFLSFRDRLDS
                     GSGFQSFQFRELEFVLGWKDPKALERYPAGGAARRRLEGRLAAPALWDAFLRYLAANG
                     VAVPKTDLARDVTRKVEPSADLRRVLVEMYRHRPELAALCERMVDLDEGLMEWRYRHV
                     KMVQRTIGTRRGTGGSDGAEYLKTTLDKPLFPDLWAIRTEL"
     gene            911..2104
                     /locus_tag="VFU45_09520"
     CDS             911..2104
                     /locus_tag="VFU45_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359348.1"
                     /translation="MDPASLYDTPNALAKHYTRFRVAERLLLTGHSHQAWPDVAFEAQ
                     AESWLDAARYVDDKWEHAFRKADRVRAGFARLLGDAGGGIALAGSTHELLVRLLSALP
                     IRARPRLVTTDGEFHTIRRQLDRLAEEGVEVVRVAESPVGSVAERLAAAVDDRTAMVL
                     VSAVFFDTGRLARGLGTVAERCRRHGAALLVDVYHALNVVPYVLRDEGLSEAYVVGGG
                     YKYCQLGEGNCFLRIPAGCALRPVVTGWYSEFTSLAEAGAGGRVAYGTGGDRFAGATY
                     DPASHYRAARVFEFFEDQGLTPATLRAVSQHQVGVLAQTFDALDLDPAVVTRDRTAPL
                     SEIGGFLALRAPAAGQLSAALHALGVLTDARGEVLRFGPAPYLSDRQLQDAMGVLGEA
                     MNGER"
     gene            2427..>3067
                     /locus_tag="VFU45_09525"
     CDS             2427..>3067
                     /locus_tag="VFU45_09525"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU5359349.1"
                     /translation="MALVLVVAGAIAATPLSPSLVSGLVWRNVGPFRGGRVSAVTGAI
                     GEPGVFYAGYPSAGVWKTVNAGITWEPISDNAITASPSVGAIEVAPSDTSVIYVGMGD
                     MITGGAINEGDGVYKSTDAGRTWSHLGLDATTQIPSILVDPKDPNLVMVAAQGNIHKR
                     SDDRGVYRSTDGGKTWTKTLFVDDTTGIQKITWAFDQPNVVFATTVKHFVEPGP"
BASE COUNT          463 a         1127 c         1004 g          473 t
ORIGIN      
        1 gatccgtggg gcaccccgct ccggatcgag gctgccaccc gctgacccgc ccgctccttc
       61 ccggccccgg ccgcggtcgc tacgttccgc caccctgagt cccccgccgg accatcccgg
      121 aaccgccatg ccgacctatt ccgaatacct caagatcaac gagctgctcg agctccagcg
      181 tcccctgtcc gacggacctg agcacgacga gatgctcttc atcgtgatcc accaggtgta
      241 cgagctctgg ttcaagcagg tcctgcacga gatcggccac ctcgaatcgc tcctcggccg
      301 gaacgacggc caccgcgccc agcacaccct caagcggatc ctcaccatcc tcaaggtgat
      361 ggtgggccag ctcgacgtcc tcgagacgat gacgccgctc gagttcctct ccttccgcga
      421 ccggctcgat tcggggagcg gcttccagtc cttccagttc cgtgagctcg agttcgtgct
      481 gggctggaaa gatccgaagg ccctggagcg ctaccccgcg gggggcgccg cccgtcggcg
      541 attggagggt cggctcgcag cgccggccct ctgggacgca ttcctccggt acctcgcggc
      601 caacggcgtg gccgtcccga agaccgacct cgcccgcgac gtgacccgga aggtcgaacc
      661 ttccgcggac ctccgccgcg tgctggtcga gatgtaccgc caccgccccg aactcgcggc
      721 gctctgcgaa cggatggtcg acctcgacga ggggctgatg gagtggcgct accgccacgt
      781 gaagatggtc cagcgcacca tcggcacccg gcgcggcacc ggcggcagcg acggcgcgga
      841 gtacctgaag acgacgctcg acaagcccct cttccccgac ctctgggcca tccgcaccga
      901 gctgtagcga atggatccgg cctccctcta cgacacgccc aacgcgctcg cgaagcacta
      961 cacccgcttc cgggtagcgg agcggctgct cctgaccggt cattcccacc aggcctggcc
     1021 cgatgtcgcc ttcgaggccc aggccgagtc gtggctcgat gccgccaggt acgtggacga
     1081 caagtgggag cacgcgttcc ggaaggccga ccgggtccgg gccggctttg cccgcttgct
     1141 gggagatgcc gggggcggga tcgcgctcgc gggaagcacc cacgaactcc tggtgcgcct
     1201 cctctccgcg ctccccatcc gcgcgcggcc ccggctcgtc accacggacg gcgaattcca
     1261 caccatccgc cgccagctcg accggctcgc cgaggagggg gtcgaggtcg tgcgggtggc
     1321 ggaatccccg gtgggatcgg tcgcggagcg gctggcagcg gccgtggacg accgcaccgc
     1381 gatggtgctc gtctcggcgg tgttcttcga caccgggcgg ctggcgcgcg ggctcggtac
     1441 cgtggccgag cggtgccgcc ggcatggcgc agcgctcctg gtcgacgtct atcacgcgct
     1501 caacgtggtc ccttatgtgc tcagagacga gggactgagc gaggcctatg tcgtcggcgg
     1561 aggctacaag tactgccagc ttggcgaggg gaactgcttc ctccggatcc cggcggggtg
     1621 cgccctgcgt ccggtcgtca ccggctggta cagcgaattc acctccctcg ccgaggccgg
     1681 cgccgggggc cgggtcgcgt acggtacggg cggcgaccgg ttcgccggcg cgacctacga
     1741 tcctgccagc cattaccgcg ccgcgagggt attcgagttc ttcgaggacc aggggctcac
     1801 gccggccacg ctccgcgcgg tgagccagca tcaggtcggc gtgctggcac agacgttcga
     1861 cgcgctcgac ctcgatcccg cggtcgtcac ccgcgaccgg accgccccgc tctctgagat
     1921 cggcgggttc ctggcgcttc gcgccccggc ggccggtcaa ctctcggcgg cgctccacgc
     1981 cctcggcgtg ctgaccgacg cccgggggga ggtgctccgg ttcggccccg cgccatacct
     2041 gtccgatcgt caactgcagg atgcgatggg ggtgttgggg gaggcgatga acggtgagcg
     2101 gtgagcggta agcggtaaga ggtaagaggt aagaggggac gaggggcgga ggggacgagg
     2161 cggcgacctc taaagacaag acagcggggc gcagggagcc tgaacgacgt tccacctgcc
     2221 tagcgtctcc cccggatggc cgcttcgaaa cgttccaggc ctcccgcgcg tacattctct
     2281 gacccgctca cacgctgacc cgctgtcagc tgatcgcccc cctgtcgcgc cggccgcatg
     2341 gcgcgcactt tacctgtctc ccattccccc atcagggctc tgatcccatg accgcgcgta
     2401 cctctggctc tttgcgactt cttggtgtgg cgctggtgct cgtcgtggcc ggcgcgatcg
     2461 ccgcgacgcc cctctcgccc agcctcgtct ccggcctcgt ctggcggaac gtcggcccct
     2521 tccgcggcgg ccgggtgtcg gccgtgaccg gcgccatcgg tgaaccgggc gtcttctacg
     2581 ccggctaccc gtccgccgga gtctggaaga cggtcaacgc cggtatcacc tgggagccga
     2641 tctccgacaa cgccatcacc gcctcgccct cggtcggcgc catcgaagtg gccccctccg
     2701 acaccagcgt catctacgtc ggcatgggcg acatgatcac cggcggcgcc atcaacgagg
     2761 gcgacggcgt ctacaagtcg accgacgccg ggcgcacctg gagccacctg ggcctcgacg
     2821 ccaccaccca gatcccctcg atcctcgtcg acccgaagga cccgaacctg gtgatggtcg
     2881 ccgcgcaggg gaacatccac aagcggagcg atgaccgcgg cgtctaccgc agcaccgacg
     2941 gcgggaagac ctggaccaag accctcttcg tcgacgacac cacgggcatc cagaagatca
     3001 cctgggcctt cgaccagccg aacgtggtct ttgccaccac ggtcaagcac ttcgtcgagc
     3061 cggggcc
//
LOCUS       DASUAP010000353         4131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_189909, whole genome shotgun sequence.
ACCESSION   DASUAP010000353 DASUAP010000000
VERSION     DASUAP010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189909"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFU45_09530"
     CDS             <1..759
                     /locus_tag="VFU45_09530"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359350.1"
                     /translation="CHASDPLTLAIALHKGRTKEVLAQRGVPTAPFLLVESAAAARGV
                     SLPFPLFAKPAFEGSGKGVSVKSLCHHRGELVAQVEHLLETYRQPVLIETYLPGPEFT
                     VAIIGNGAEARCLPIVGFRFDTLPAGAPPIYGYEAKWIWDTVQSPLEIFQCPADIPDV
                     QADAVRAAALGAYRALECRDWCRVDVRCDAAGQPMVVELNPLPGILPDPRDNSCFPKA
                     ARAAGMDYDALIQAVADIAWRRISGRSLLSEVAR"
     gene            756..1763
                     /locus_tag="VFU45_09535"
     CDS             756..1763
                     /locus_tag="VFU45_09535"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359351.1"
                     /translation="MKGAILYDPGGKHWDKKDVAQVAENAEEIQANLKSRGIQVSLIP
                     IHLGDFSWIAKARRVDVIFNLCEGLDGHSKYEDYVVGTLELMGVPYTGCRPWPVTVCH
                     RKHIANTLMQAAGVPIPKFWLAQGNKLPSEFPLPAIVKPAAEDASLGIDSGAVCTSKK
                     GLKKRLAEQLEQWDEVLVQEYVAGREFNVGFVGHQALPLSEICFDGMPEGSWPVLTYS
                     AKWDEGSAEDLGSVPVCPADIPADVAKRIVAAARQAWELLAGGEGYGRVDMRLDARGQ
                     PWVLEVNPNPDLSDNAGLSRMARANGWDYGELLQKIVDEALARVARKSAANDLAQAVN
                     Q"
     gene            1760..2263
                     /locus_tag="VFU45_09540"
     CDS             1760..2263
                     /locus_tag="VFU45_09540"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5359352.1"
                     /translation="MSRTLTAPVLRALRPGDRDAIERMTRDVGVFKAHEVPVALEVFD
                     ASLRAGQTDYETQGAELDGRLAGWICWGRTPCTEGTWDLYWLAVDPAVHGAGVGTALV
                     EEMERRLAGRARLIVVETGGRAEYDPTRAFYLARGYHVAATIPDFYAPGDAQVVFVKT
                     LTSPSRA"
     gene            2278..3456
                     /locus_tag="VFU45_09545"
     CDS             2278..3456
                     /locus_tag="VFU45_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEU5359353.1"
                     /translation="MPSTWQEVLKERSIHSLEQLAERFGAENFPELDRLKLAAENFEF
                     RISPAMIDLIKEPNDPIWRQYIPSVQELDVKDGIVDSLNEDADSPVPNITHRYPDRAL
                     FLVSPVCATYCRFCTRRRKVGDPEKIPMSQFESAFKYLEEHTEIRDVIMSGGDPLLLN
                     DRRLEMILTRLRSIKHIQIIRIGSRVPCHLPERITPELCATLRQFHPLWINTHFNHPD
                     ELTTAAIAGLGMLADAGIPLGCQTVLLKGVNDDPAVMMELMQKLMIARVRPYYIYMAD
                     QVAGAEHFRTTVETGLKIIKALRGWTSGLAVPHFVIDAPGGGGKIPLLPEYVESINED
                     EVVLRNFRGDRYTYRQPEQDDKETRIHDPEYLFQPAMERKKARKRSPKVKVAAKRKSA
                     "
     gene            complement(3794..>4131)
                     /locus_tag="VFU45_09550"
     CDS             complement(3794..>4131)
                     /locus_tag="VFU45_09550"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEU5359354.1"
                     /translation="TLRATGTGWTFIALVLSHGVSFVMDYLLGGEYRRVELRQLLTQP
                     YDRVFILELAVMVGAYVVAELHSAVPALIVLVGFKLSADLHAHRRERRKLRAPSKARM
                     FAAAWGDPA"
BASE COUNT          675 a         1387 c         1475 g          594 t
ORIGIN      
        1 tgccacgcct ccgacccgct gaccctggcc atcgccctcc acaaggggcg gaccaaggag
       61 gtcctggcgc agcggggggt gccgacggcg ccgttcctgc tggtcgagtc ggccgccgcg
      121 gcccggggcg tctcccttcc cttcccgctc ttcgccaagc cggcgttcga ggggtcgggc
      181 aagggggtca gcgtcaagtc gctctgccac caccgggggg agctggtggc ccaggtggag
      241 catctgctcg agacctaccg ccagccggtc ctgatcgaga cctacctccc cggccccgag
      301 ttcaccgtcg ccatcatcgg caacggcgcc gaggcgcgct gccttccgat cgtcgggttc
      361 cgcttcgaca ccctgccggc cggtgccccg ccgatctacg gctacgaggc gaagtggatc
      421 tgggacacgg tccagtcgcc gctcgagatc ttccagtgcc cggcggacat ccccgacgtg
      481 caagccgatg cagtccgggc ggcggcgctc ggcgcctacc gcgctctcga gtgccgggac
      541 tggtgccggg tggacgtgcg gtgtgacgcc gccggccagc cgatggtggt cgagctcaac
      601 cccctcccgg ggatcctccc cgacccccgc gacaactcct gcttccccaa ggcggcgcgg
      661 gcggcgggta tggactacga cgcgctgatc caggccgtgg cggacatcgc ctggcggcgg
      721 atcagcggcc ggtccctcct ctccgaggtg gcgcgatgaa gggtgcgatc ctgtacgacc
      781 ccggcggtaa gcactgggac aagaaggacg tggcccaggt ggcggagaac gccgaggaga
      841 tccaggccaa cctcaagtcg cgcgggatcc aggtgtcact catcccgatc cacctcggcg
      901 acttcagctg gatcgccaag gcgcgccggg tggacgtgat cttcaacctc tgcgaagggc
      961 tggacgggca ctcgaagtac gaggactacg tggtcggcac cctggagctg atgggggtgc
     1021 cctacaccgg gtgccgcccc tggccggtga ccgtctgcca ccgcaagcac atcgccaata
     1081 ccctgatgca ggccgccggg gtgccgatcc ccaagttctg gctggcccag gggaacaagc
     1141 tgccgagcga gttcccgctc ccggccatcg tgaagccggc ggcggaggac gcgagcctgg
     1201 ggatcgacag cggggcggtc tgcacttcca agaaggggct caagaagcgc ctggccgagc
     1261 agctcgagca gtgggacgag gtgctggtgc aggagtacgt ggcggggcgc gagttcaacg
     1321 tgggattcgt gggacaccag gcgctcccgc tctcggagat ctgcttcgac gggatgccgg
     1381 aggggagctg gccggtgctc acctactcgg ccaagtggga tgagggaagc gccgaggacc
     1441 tgggctcggt gccggtctgc ccggcggaca ttccggcgga tgtggccaag cggatcgtgg
     1501 ccgcggcgcg gcaggcgtgg gagctgctgg cggggggcga agggtacggc agggtggaca
     1561 tgcggctcga cgcgcggggg cagccgtggg tgctcgaggt gaaccccaac cccgacctgt
     1621 ccgacaacgc cgggctctcc cggatggcca gggccaacgg gtgggactac ggcgagctgc
     1681 tgcagaagat cgtggacgag gcgctggcgc gggtggcgcg gaagtcggcc gccaacgacc
     1741 tggcccaggc ggtcaatcag tgagccgcac gctcaccgca ccggtgctcc gtgcgctccg
     1801 ccccggggac cgggacgcga tcgagcggat gacgcgggac gtgggcgtct tcaaggcgca
     1861 cgaggtgccg gtggcgctcg aggtgttcga tgcctcgctc cgggccggcc agacggacta
     1921 tgagacccag ggggccgagc tcgacgggcg gctggccggg tggatctgct gggggcggac
     1981 cccctgcacc gaagggacct gggacctcta ctggctggcc gtcgaccccg cggtgcacgg
     2041 cgcgggggtg gggacggcgc tggtggagga gatggagcgg cggctcgcgg gccgcgcgcg
     2101 cctgatcgtg gtggagaccg gcgggcgggc ggagtacgac ccgacccgcg ccttctatct
     2161 ggcccgcggc taccacgtgg ccgccacgat ccccgatttc tacgccccgg gcgatgccca
     2221 ggtcgtgttc gtcaagacgc tcacttcacc gtcccgcgcg taagggagac gttcgttatg
     2281 ccgtccacgt ggcaggaagt cctcaaggag cgctcgatcc actcgctgga gcagctggcg
     2341 gagcggttcg gcgccgagaa cttccccgag ctcgaccggc tgaagctggc ggcggagaac
     2401 ttcgagttcc gcatctcgcc ggcgatgatc gacctgatca aggagccgaa cgacccgatc
     2461 tggcggcagt acatcccgtc agtgcaggag ctcgacgtga aggacgggat cgtcgactcg
     2521 ctcaacgagg acgccgattc cccggtgccg aacatcacgc accgctatcc cgaccgtgcg
     2581 ctcttcctgg tgtccccggt gtgcgccacc tactgccgct tctgcacccg gcggcgcaag
     2641 gtgggcgacc ccgagaagat cccgatgagc cagttcgagt cggccttcaa gtacctcgag
     2701 gagcacaccg agatccgcga cgtcatcatg agcggcggcg accccctgct cctcaacgac
     2761 cggcggctgg agatgatcct gacccggctc cggagcatca agcacatcca gatcatccgg
     2821 atcgggagcc gggtgccctg ccacctcccc gagcggatca cgccggagct ctgcgccacg
     2881 ctccggcagt tccacccgct ctggatcaac acccacttca accaccccga cgagctcacc
     2941 acggcggcga tcgcggggct cggcatgctg gcggacgccg gcatccccct cggctgccag
     3001 acggtgctcc tgaagggcgt caacgacgac cccgccgtga tgatggagct gatgcagaag
     3061 ctgatgatcg cccgggtccg gccctactac atctacatgg ccgaccaggt ggcgggggcg
     3121 gagcacttcc gcaccaccgt cgagaccggg ctcaagatca tcaaggcgct ccgcgggtgg
     3181 accagcgggc tcgcggtgcc gcacttcgtg atcgacgccc ccgggggcgg cgggaagatc
     3241 ccgctcctgc cggagtacgt cgagagcatc aacgaggacg aggtggtgct ccgcaacttc
     3301 cgcggcgacc gctacaccta ccgccagccg gagcaggacg acaaggagac ccggatccac
     3361 gacccggagt acctcttcca gccggcgatg gagcggaaga aggcgcggaa gcggtcaccc
     3421 aaggtcaagg tggcggcgaa gcggaagagt gcgtgaccac cgcgcgctga ggcgcagggg
     3481 cggcgcatgc gccgcccgtc cgatgccagg cggtgaatgg ggctccggtg atgaccgggg
     3541 ccccgttttc attggtgggc tcgagggtgg atggcatggc gatcgatcaa ggactgcttg
     3601 aaccacggag ggcatccttc gcttcgctca ggacaggttc cggagcacga agaggcacga
     3661 agaactgcac ttgatgtggt cagccggccg ggactccgct cgcgcttcgg ggtcattccg
     3721 aggggagcga ggaatctcgc cagccatgga tgcccgtgtc ccggccgcgc cgaaggtcat
     3781 tcgccgtgcg ccttcaggcg ggatctcccc acgccgctgc gaacatccgg gccttggatg
     3841 gcgcccggag cttccgtcgt tcacgccggt gtgcgtggag gtcggcgctg agcttgaacc
     3901 ccaccaggac gatcagcgcc gggaccgccg aatggagctc ggcgaccaca taggccccga
     3961 ccatgacggc gagctccagg atgaagaccc gatcgtaggg ctgggtcagc agctgccgca
     4021 gctcgacgcg tcggtattcc ccaccgagca ggtagtccat cacgaatgag acgccatggc
     4081 tcaggacgag ggcgatgaac gtccagcccg tgccggtggc acgcagcgta g
//
LOCUS       DASUAP010000354         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_434538, whole genome shotgun sequence.
ACCESSION   DASUAP010000354 DASUAP010000000
VERSION     DASUAP010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434538"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFU45_09555"
     CDS             <1..462
                     /locus_tag="VFU45_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5359355.1"
                     /translation="LIAGTRAGAERILATLDSVGTHPAVLAGDGVVGIEAGGKAEGMY
                     VSSAYLPDRPGDKNAAFVKAYQARYPGQLPDHRGAGAYDVIYLLKRAIESGATSRAAI
                     RDYLAGVGTKTPAFDGVTGSIAFDEHRDVPAKDVVIGQVRNKRLVTVAAGK"
     gene            562..1686
                     /locus_tag="VFU45_09560"
     CDS             562..1686
                     /locus_tag="VFU45_09560"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEU5359356.1"
                     /translation="MRPRQLLVKLHLWVGMTAAPFLLILGLTGALLVFEEPIQDAFNA
                     RLALVTPVGTAHSFADLEQRLVAAHPGYRVAAVTYPRDARHSYAIGLTPLNGTGDDLE
                     YFVDPYTANVLGTPAEERGPMPVIHQLHTRLLAGHKGNVVVGWAAVMLVFLSLSGLVL
                     WWPGKIFTVRWSGTAKRVTFDLHNALGAYAWVFLFLLAMTGLVIHWNELAAGPAGTGG
                     AGAGVVFIPRPAAGGPPSLPPLPPPAPACDAARRLPFDRVVAMGAAALPGTSVVLAQE
                     IDPTRGTAMVALRYPEDRTPAGRSRVQVDECTGAIVSTQDIRAQPARFVYARMTNRSI
                     HTGDIFGWPTRILAALMSLTLPLMAITGPLIWWTRRAPRR"
     gene            complement(1683..>3148)
                     /locus_tag="VFU45_09565"
     CDS             complement(1683..>3148)
                     /locus_tag="VFU45_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEU5359357.1"
                     /translation="PGVIRWSDATTVPAWARAVEALALSPTRGVAELIRGVLWAAAAS
                     SVAALAGCVRRYAALLDHAADARAAGRMLTREELRTFTGPMGGQLAMWELITPGCGST
                     DLDLDDRERLPALRALLEQELASVDAKLDRLRALLADGVPTIVFTGSVDTVGYLRTRL
                     AGVAWCTGTSAGIGHLHADREAILGAFAPGRAGPGVLIATDIAAEGLDLHRAARVVHF
                     DLPWTPMRLRQREGRVARLGAERSSVDVIWFRPPEWLDRKERRALRLRQKARAVVEAG
                     FHHRAPDLWRWRHELAAGAGSPAPFGHCATVRGARAELLIAFELQRGSERLGSILGIV
                     NEAGEWIDDPSAIVDAVGRARAGVTTQVPPDEWNRWLERALPHARAVVRRANDLTWSW
                     SELRPEARALVRRLRREAADAARRRDARELALLDQGLAFATRGHTAGETAQVSGMVES
                     PRSGLLRALSRLQEQTRERRPITLRVAGMILFVPQET"
BASE COUNT          451 a         1157 c         1083 g          457 t
ORIGIN      
        1 ctgatcgccg ggacgcgcgc gggggcggag cggatcctgg ccacgctcga cagcgtgggg
       61 acccatccgg cggtcctcgc cggcgacggc gtcgtcggga tcgaggcggg ggggaaggcg
      121 gaggggatgt acgtctcgag cgcgtacctg cccgaccggc cgggcgacaa gaacgccgcc
      181 ttcgtgaagg cgtatcaggc caggtacccg gggcagctcc ccgaccaccg gggggcgggg
      241 gcgtacgacg tcatctatct attgaagcgg gccatcgagt cgggggccac gagccgggcg
      301 gccatccgcg actacctggc gggcgtgggc accaagaccc cggcgttcga cggcgtcacc
      361 ggcagcatcg cgttcgacga gcatcgggat gtgccggcca aggacgtggt catcggccag
      421 gtgcggaaca agcggttggt gacggtggcg gcggggaagt aggttcgggc agctatcagc
      481 tatcagcgac aaggtatccg cgaaccgctc accgctcact gctaacagcc atcagcgccc
      541 ggccacgaac cgccgatccg aatgcgccca cgccagctcc tcgtcaagct ccacctctgg
      601 gtcggcatga ccgccgcccc gttcctcctc atcctcggcc tgaccggcgc gctcctcgtc
      661 ttcgaggagc cgatccagga tgcgttcaac gccaggttgg cgctggtcac gccggtcggc
      721 accgcgcact cctttgccga cctcgagcaa cgcctggtcg cggcgcatcc gggataccga
      781 gtggcggcgg tcacctatcc ccgtgatgcg cggcactcct atgccatcgg tctcacgccg
      841 ctcaacggca ccggggacga ccttgagtac ttcgtcgatc cctacaccgc caacgtcctc
      901 ggcacgccgg ccgaggagcg ggggccgatg cccgtcatcc accagctcca cacccggctc
      961 ctggcggggc acaaggggaa cgtggtcgtc ggctgggccg cggtgatgct cgtcttcctc
     1021 tcgctcagcg ggctcgtcct ctggtggccg ggcaagatct tcacggtccg atggagcggc
     1081 acggccaagc gggtcacctt cgacctgcac aatgcgctcg gcgcctatgc gtgggtcttc
     1141 ctgttcctcc ttgcgatgac cgggctcgtc atccactgga acgagctggc ggcggggccg
     1201 gcggggacag ggggcgccgg cgcgggcgtc gtgttcattc cgagacccgc ggccggtgga
     1261 ccgccgtcgc ttccgcccct cccgccgccc gcaccggcgt gcgatgccgc ccggcgcctt
     1321 cccttcgacc gggtggtggc gatgggagcg gcggcacttc ccgggacgtc ggtggtgctg
     1381 gcgcaggaga tcgatccgac gcgtggcacc gcgatggttg cgctccgtta ccccgaggac
     1441 cgcacgccgg cgggtcgctc gcgggtccag gtcgatgaat gcaccggagc catcgtctcg
     1501 acgcaggaca tccgcgccca gccggcgcgt ttcgtgtacg cgcggatgac caaccgctcc
     1561 atccacaccg gcgacatctt cggctggccc acccggatac tggcggcgct gatgagcctc
     1621 acgctcccgc tgatggcgat caccgggccg ctcatctggt ggacccggcg ggcgccgcgc
     1681 cgctaggtct cctgcggcac gaacaggatc atccctgcca ccctgagcgt gatcggccga
     1741 cgctcccgcg tctgctcctg cagccttgac agcgctcgca ggagaccgga gcggggcgac
     1801 tccaccatcc ctgatacctg cgccgtttct cccgcagtgt gaccgcgtgt ggcgaacgcc
     1861 aggccttggt ccagcagtgc gagctcccgt gcgtccctcc gacgcgcggc atctgcggcc
     1921 tcccgccgca aacgccggac gagtgcccga gcctccggcc gaagctcgga ccacgaccac
     1981 gtgagatcgt tcgcccgacg cacgactgcg cgggcgtggg gcagggcccg ctcgagccac
     2041 cggttccact catccggcgg gacctgggtc gtcacgccgg cacgcgcgcg accgacggcg
     2101 tcgacgatgg cgctcggatc gtcgatccat tcccccgcct cattcacgat cccgagtatc
     2161 gatcccaggc gctcgctgcc gcgctgcagc tcgaacgcga tgaggagctc ggcgcgcgct
     2221 ccccgcacgg tggcgcaatg cccgaaaggt gccggactcc cggcgcctgc ggccagctcg
     2281 tgacgccagc gccacaagtc gggcgcccgg tggtggaatc cggcttcgac caccgcgcgc
     2341 gccttttgcc gaagccggag ggcccgtcgt tcctttcggt ccagccattc cggtggccgg
     2401 aaccagatca cgtccacgct ggatcgctcg gcgccaagcc gggcgaccct cccctctcgc
     2461 tggcgcaggc gcattggcgt ccatgggagg tcgaaatgca ccacccgtgc cgcccggtgg
     2521 aggtccagcc cttcggccgc aatgtcggtc gcgatgagca cgccgggccc cgcacgtccg
     2581 ggcgcgaagg cgccgaggat cgcctcccgg tcggcgtgaa ggtggccgat ccccgccgat
     2641 gtcccggtgc accacgccac gccggcgagg cgggtcctca ggtaccccac ggtgtcgacg
     2701 gatccggtga acacgatcgt cgggacgccg tccgccagca gcgcccgaag gcggtcgagc
     2761 ttggcgtcga ccgaggcgag ctcctgttcg agcagcgcgc ggagcgccgg gagccgctcg
     2821 cggtcatcca ggtcgaggtc ggtcgagccg cagcccggcg tgatgagttc ccacatcgca
     2881 agctggccgc ccatcggacc ggtgaacgtc cgcagttcct cccgggtcag catgcgaccg
     2941 gcggctcgtg cgtcagcggc atggtcgagc agcgccgcat accgccggac acatccggcg
     3001 agcgccgcaa cactcgacgc cgccgcggcc cacagcacgc ccctgatgag ctcggcgaca
     3061 ccccgtgtgg gcgagagcgc cagcgcttcg acggcccggg cccaggccgg gacggtcgtg
     3121 gcatcggacc agcggatcac tccgggtg
//
LOCUS       DASUAP010000355         7550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1033836, whole genome shotgun sequence.
ACCESSION   DASUAP010000355 DASUAP010000000
VERSION     DASUAP010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033836"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFU45_09570"
     CDS             <1..230
                     /locus_tag="VFU45_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU5359358.1"
                     /translation="VIPVKHLAAARDARTPEDRALLGRLVAFATEVATEMGLDAGGYR
                     LVTNTGADGGQTVGHLHLHLLGGRKMTWPPG"
     gene            complement(370..843)
                     /locus_tag="VFU45_09575"
     CDS             complement(370..843)
                     /locus_tag="VFU45_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5359359.1"
                     /translation="MPSAPPLRLRNFDYSAPGAYLITITTAGRSPSLGRLTQGGVELS
                     ALGKLVAQELGRTEALRSGVIMDAVAIMPDHIHFIVLLTDQAPGGSNISRVVNGFKAA
                     VTSGYRRRGGDQTAHIWQRGFHDRVIRSDRDLAEARRYVLENPAAAWLKQVSHAR"
     gene            1196..1393
                     /gene="rpsU"
                     /locus_tag="VFU45_09580"
     CDS             1196..1393
                     /gene="rpsU"
                     /locus_tag="VFU45_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU5359360.1"
                     /translation="MSEVIIHDDESFERALKRFKKKCEKAGILSDLRKHRHYEKPSEK
                     RKRKQNAAIRKNRRGPRIPRV"
     gene            1386..1826
                     /locus_tag="VFU45_09585"
     CDS             1386..1826
                     /locus_tag="VFU45_09585"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU5359361.1"
                     /translation="MSDLFDRLQGALTGARKAQDKERTLLLSTIFAAVKNREIELKRS
                     LTDDDVVEVLRKGVKTRQDSVEQFEKAGRAELAAREAAQIKMLEEFLPASVDPAEIRA
                     AARAAVAAGAKDVGALMKVLMPQFKGRADGKLINQVAREELPAG"
     gene            1865..4333
                     /locus_tag="VFU45_09590"
     CDS             1865..4333
                     /locus_tag="VFU45_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEU5359362.1"
                     /translation="MPTGAGTPSVLPPEHSAAALAALEFPAALDAVAAFAAGPLGAQR
                     IRSRRPSADLVAVREELAAVEEVAALERAGRGLAIAAAPDAAPALSRLRREGSVLEGV
                     ELALVARLLEAGRRTSDEIGRVALEAPRVARLAVPLPTPELDRRLARSIDADGAVLDT
                     ASPALAEARREVQSARTKLLKRLEALLRGADGAPDEAAITLRNGRYVIPVRRDSRKRP
                     DGIVHDESASGTTLFIEPAETIPLGNALREAEVAEEREVRRVLAELSALLRPWAPSLA
                     GVQEMCIAVDDLAARGRYAARCDAVAPEIGGPGSAMVIRRGRHPILLARGIAVVPFDL
                     VLERGERTVLVSGPNTGGKTVFLKAVGLLAALAQSGIVPPLAAGSSLPLFRQFHADIG
                     DHQSIAADLSTFSAHVAEVRRILDGADDATLVLFDEIGSGTDPAEGAALAAATLTALT
                     TRGVTTIATTHLGALKDLATRIGGVVNASLQFDVAALAPTYRFQKGVPGRSYGLAIAR
                     RLGVDPAVLAAAESLVSTEERRLDQLLAAAEAKGAELDRRARELDDRALDAENLAARL
                     AAQESAQAAREAALTAREKEAEREKRSRSRDYLLEARQTVEDALTLARKAATEEEARE
                     ARRKVEAAALSEREALEQLETGAESEVTGGARAAGGGAEVGVGDLVRTSGGVSGEVIE
                     VRRDGKVVISAGAVRLTVDAAELSRVPGRTSRVAGRASHVSRPVTRDSRPATHDPRPA
                     THDHEIDLRGMTGDEAEMVTAAAVDAAVLGEAPFLRIIHGMGTGVVRERVQRVLKADK
                     RVKAFAFAPRNQGGTGVTVAEFTP"
     gene            4330..6171
                     /gene="dnaG"
                     /locus_tag="VFU45_09595"
     CDS             4330..6171
                     /gene="dnaG"
                     /locus_tag="VFU45_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU5359363.1"
                     /translation="MSRIPDEVIEQVRDTADLVAIIGESVELKRTGTDYRGPCPFHGG
                     TNRNFAVVPKRGMYYCFVCHESGDVFKYLMKRFGMDYPTAVRAVASKSGIVIPETTER
                     AGPDPREPLYSAVATAHDWFQRQLRELPEAEAARAYVAKRGLTPEQAAELGLGYAPAG
                     PAFQQAMTALGISAATLLEAGLTAERQDGSVGPRFRQRLLIPIHDLRGRVVAFGGRIL
                     GEGEPKYLNSPESPVYHKGEMLYNLHAAKQSIRKEEMAIVVEGYFDTLRLVLAGVENV
                     VAPLGTSLTDRQAALLRKFTPAVTVLYDSDGPGLRAAFRAADELLRHGVRVRVATLPD
                     DEDPDSLVETQGVEALRKVLHDAEDVIERKIGMLERKGWFADVEHRREALDRLLPTIR
                     ATSDPITRELYLTTVAERAKVSRDVLQAELAKRADNRMGGRSDDAGRHGPGAPSDRPS
                     PRPSDAASSRPSDRPTPRRRSGDEKSFLRVLLALPEWRERARQDAVKPEWFERKEYRE
                     VFAQLLAGAALPDALSDEAQLAWSELQASAHGLTENAGDVYEAAREALEARPLLRQFD
                     ALQAQIDVAGPDELEVLLRRKEEMRADLKRRFPNEWQRRPLRRAGRR"
     gene            6235..6948
                     /locus_tag="VFU45_09600"
     CDS             6235..6948
                     /locus_tag="VFU45_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359364.1"
                     /translation="MTDELVKLLDLQAKDLALLELDVRSLEIDEEEAALEREVAGAVG
                     KAAQAEREAKDAAARRDELEQRIESYKKLNDRRRQRLETVRNPKEAATLMAELDLAQS
                     VVKREEGEWVKAADAVALAEERSKLAAAQVAEMRGAQSEARDAVAAKRKALEGDRAAA
                     SAAREAAAKGVEKPLRSRYERLFNSRKRPVVVALVGVSCSACRTAVPTSRRSPTSGGL
                     LADPCESCGVILYRLAVEG"
     gene            6982..>7550
                     /locus_tag="VFU45_09605"
     CDS             6982..>7550
                     /locus_tag="VFU45_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU5359365.1"
                     /translation="MTSAALVTRFPVPPLRWVVAPRAEPALARELAAQLSVPLPLAEL
                     LVQRGFADRDSARKFLKPALDGLSDPYAMAGMRGAVDAVVRAIHAQQTILVHGDYDVD
                     GQCATALLTRVLRAAGARVVPFIPHRMGDGYDFGPAGVKAAADAGASLIITCDCGITA
                     VDAVRSARAAGREVIVTDHHLPGPALPDAT"
BASE COUNT         1116 a         2707 c         2752 g          975 t
ORIGIN      
        1 tggtgatccc ggtcaagcac ctggccgccg cccgcgatgc gcggacgccg gaagaccggg
       61 cgcttttggg gcggctcgtc gcattcgcga ccgaggtcgc caccgagatg ggcctcgatg
      121 ccggcgggta ccggctggtg accaacaccg gcgccgatgg cggccagacc gtcgggcacc
      181 tccatctgca cctgctcggc gggcggaaga tgacctggcc gccggggtag gggtgataca
      241 ctgtgggggt cgcctgcggc gacccgaagc tgcctgaagt gaccgactgt gggggtgggg
      301 gtcgcctgcg gcgacccgaa gctggctgaa gtgaccgact gtgggagtgg gggtcgcctg
      361 cggcgaccct catcttgcgt gtgacacctg tttgagccat gccgccgctg gattctccag
      421 cacatatcgg cgagcctccg caagatctcg gtccgaccgg atgacccggt cgtgaaatcc
      481 gcgctgccag atatgagcag tctgatcccc gcctcgtcgg cgatagcccg acgtcacggc
      541 agccttgaac ccattcacca cgcggctgat gttggagccc ccgggcgcct gatccgtcaa
      601 gagcacgatg aaatggatgt ggtcgggcat gatcgccacg gcgtccatga tgacgccgga
      661 gcgaagcgct tctgtgcgcc cgagctcctg ggccacgagc ttccccaggg cactgagctc
      721 cacgccgccc tgggtcagcc ggccgagcga cggggaccga cctgcggtgg tgatggtgat
      781 caggtaggcc ccgggtgcgg agtagtcgaa gttgcgaagc cgaagcggtg gggcggaggg
      841 catgatacaa tctgggattg gcgtccccga ctaccatcat ccggcgaatg cagggcgggg
      901 ccgccggttg cggagtctgg gtcgccgcag gcgaccccca cacccacaac taggaaccaa
      961 cgccggcttc gggtcgccgc gggcgacccc cacacccgca gctaggaacc ttagccggct
     1021 tcgggtcgcc gcaggcgacc cccacaccca cagctaggaa cctcagccgg cttcgggtcg
     1081 ccgcaggcga cccccacaca caagcccccg cccttgaacc ccgccggccg atgggctaag
     1141 ttcatagtct gcaaacattt gtcgccactg acgtggcccg agaggggtga tttgtttgtc
     1201 cgaagtcatc attcacgatg acgagagctt cgagcgggcg ctcaagcgct tcaagaagaa
     1261 gtgcgaaaag gccgggatcc tctccgacct gcgcaagcac cggcactacg agaagccgag
     1321 cgagaagcgc aagcggaagc agaacgcggc catccgcaag aaccgccgcg gcccgcggat
     1381 cccccgtgtc tgacctgttc gaccggctgc agggtgcgct caccggcgcc cgcaaggcgc
     1441 aggacaagga gcgcactctc ctgctgtcga cgatcttcgc ggcggtcaag aaccgcgaga
     1501 tcgagttgaa gcgcagcctc accgacgacg acgtggtcga ggtcctccgc aagggggtca
     1561 agacccgcca ggactcggtg gagcagttcg agaaggccgg ccgggcggag ctcgccgcgc
     1621 gcgaggccgc ccagatcaag atgctggaag agttccttcc cgcttccgtc gaccccgccg
     1681 agatccgtgc cgcggcccgg gcggcggtgg cggccggagc caaggacgtc ggagccctga
     1741 tgaaagtgct gatgccgcag ttcaagggac gcgccgacgg gaagctcatc aatcaggtgg
     1801 cgcgcgagga gttgccggcc gggtgacggc cgtcgctccc gcgggatccc ctcacggggt
     1861 gccggtgccc acgggcgcag gcaccccgtc cgttttgccg ccggagcact ccgccgcggc
     1921 cctcgccgcg ctcgagttcc ccgccgccct cgacgccgtc gccgcctttg cggccggccc
     1981 cctcggcgcc cagcgcatcc gctcccgccg tccgtccgcg gacctcgtcg cggtgcgcga
     2041 ggagctcgcc gcggtcgaag aggtcgccgc actcgagcgc gccggtcggg ggctcgccat
     2101 cgccgccgca cccgacgcgg cgccggccct ttcccgcctc cggcgcgaag ggagcgtcct
     2161 cgagggcgtc gagctggcgc tcgtggcccg gctcctcgag gcggggcgcc gcaccagcga
     2221 cgagatcggg cgggtggcgc tcgaggcccc gcgcgttgcc cggctggcgg tgccgctgcc
     2281 cacgcccgag ctcgaccgcc ggctggcccg ctcgatcgac gccgacggtg cggtcctcga
     2341 cacggcgagc ccggcgctcg ccgaggcgcg gcgggaggtc cagtcggcgc gaacgaagct
     2401 cctcaagcgc ctcgaggcac tcctccgcgg cgccgacggc gcgcccgacg aggcggccat
     2461 caccctccgg aacggtcgct acgtcatccc ggtgcggcgc gactcccgca agcgccccga
     2521 cggcatcgtc cacgatgagt cggcgagcgg caccacgctc ttcatcgagc cggccgagac
     2581 gatcccgctg ggcaacgcgc tccgcgaagc cgaagtggcc gaggagcgcg aggtgcgccg
     2641 ggtgctggcg gaactctccg cgctgctccg gccctgggcc ccgtcgctcg cgggggtgca
     2701 ggagatgtgc atcgccgtcg acgacctcgc ggcgcgcggg cggtacgcgg cacggtgcga
     2761 cgcggtggcg ccggagatcg gcgggcccgg gtcggcgatg gtgatccggc ggggccggca
     2821 cccgatcctc cttgcacggg ggatcgcggt cgtccccttc gacctggtgc tggagcgcgg
     2881 cgagcgcacg gtgctcgtga gcggccccaa caccggcggc aagaccgtct tcctcaaggc
     2941 ggtggggctc ctggccgcgc tggcgcagag cggcatcgtg ccgccgctcg cggcggggtc
     3001 atcgctcccc ctcttccgcc agttccacgc cgacatcggc gaccatcagt cgatcgcggc
     3061 cgacctctcg accttcagcg cccacgtggc cgaggtgcgc cggatcctcg acggcgccga
     3121 cgacgcgacg ctggtgctct tcgacgagat cgggagcggc accgatcccg cggagggggc
     3181 ggcgctcgcc gccgccacgc tcacggcgct gaccacgcgc ggcgtcacca ccatcgcgac
     3241 gacccacctc ggcgcgctca aggacctggc cacccggatc ggcggcgtcg tcaacgcttc
     3301 gctccagttc gacgtcgcgg cgctcgcgcc cacctaccgg ttccagaagg gcgtccccgg
     3361 gcgctcgtac ggcctcgcca tcgcgcggcg cctgggcgtc gacccggcgg tgctggcggc
     3421 ggccgaatcg ctcgtatcca ccgaggagcg gcggctcgac cagctgctcg ccgcggcgga
     3481 ggcgaagggc gcggagctcg accgccgggc gcgggagctc gatgaccggg cgctcgacgc
     3541 ggagaacctc gccgctcggc tcgcggcgca ggagtcggcg caggcggcgc gggaggccgc
     3601 actcacggcg cgcgagaagg aggcggagcg ggaaaagcgc tcgcgctcgc gcgattacct
     3661 gctcgaggcg cggcagacgg tggaggacgc gctgacgctg gcgcggaagg cggcgaccga
     3721 ggaggaggcg cgggaggcac ggcggaaggt ggaagccgcc gcgttgagcg agcgggaggc
     3781 gctggagcaa ctcgagacgg gtgccgagag cgaggtgacg ggtggggcgc gggcggcggg
     3841 tggcggggcc gaagtcggcg tcggagacct ggtgcggaca tcgggtggcg tcagcggcga
     3901 ggtgatcgag gtccggcgcg acggcaaggt cgtcatttcg gcgggggcgg tccggctgac
     3961 cgtcgatgcc gccgagctgt ctcgcgtgcc aggtcgcacg tcgcgggtcg cgggtcgcgc
     4021 gtcccacgtc tcacgacctg tgacccgcga ctcgcgaccc gcgactcacg acccgcgacc
     4081 cgcgacccac gaccacgaaa tcgacctccg cggcatgacc ggcgacgagg ccgagatggt
     4141 caccgccgcc gctgtcgacg ctgcggtgct cggcgaggcg cccttcctcc gcatcatcca
     4201 cgggatgggc accggggtcg tgcgggagcg ggtgcagcgg gtgctcaagg ccgacaagcg
     4261 ggtcaaggcg ttcgcctttg cgccccggaa ccagggcggc accggggtca cggtggcgga
     4321 gttcacgcca tgagccggat ccccgacgag gtgatcgagc aggtgcgcga taccgccgac
     4381 ctcgtggcca tcatcggcga gtcggtggag ttgaaacgca ccggcaccga ctaccgcggg
     4441 ccctgcccct tccacggcgg caccaaccgc aacttcgccg tcgtgccgaa gcgggggatg
     4501 tactactgct tcgtctgcca cgagagcggc gacgtcttca agtacctgat gaagcgcttc
     4561 gggatggact acccgaccgc ggtccgcgcc gtggcgtcga agagcgggat cgtgatcccg
     4621 gagaccaccg agcgcgccgg ccccgacccg cgggaaccgc tctactcggc ggtggccacg
     4681 gcgcacgact ggttccagcg ccagttgcgc gaactgcccg aggccgaggc ggcccgcgcg
     4741 tatgtggcca agcgggggct cacgcccgag caggcggcgg agctcgggct cggctacgcc
     4801 cctgccggcc ccgccttcca gcaggcgatg acggccctcg gcatatcggc ggccaccctc
     4861 ctcgaggcgg ggctcaccgc cgagcggcag gacggctcgg tgggcccccg cttccgccag
     4921 cggctcctca tccccatcca cgacctccgc ggccgggtgg tggccttcgg cggccggatc
     4981 ctgggcgagg gcgaacccaa gtacctcaac tcccccgagt cgccggtgta ccacaagggg
     5041 gagatgctct acaacctcca cgccgccaag cagtccatcc gcaaggagga gatggccatc
     5101 gtcgtcgaag ggtacttcga cactctccgc ctggtcctgg ccggcgtcga gaacgtcgtg
     5161 gcgccgctcg gcacctcgct caccgaccgg caggcggcgc tgctgcggaa gttcaccccg
     5221 gcggtgacgg tgctctacga cagcgacgga ccggggctcc gcgccgcctt ccgcgccgcc
     5281 gatgagctcc tgcgccacgg cgtccgggtg cgggtggcga cgctccccga cgacgaggat
     5341 cccgactcgc tggtcgagac gcagggggtc gaggcgctcc gcaaggtcct gcacgacgcc
     5401 gaggacgtga tcgagcgcaa gatcgggatg ctggaacgga aggggtggtt cgcggatgtg
     5461 gagcaccgcc gcgaggcgct cgaccggctc ctccccacga tccgcgccac cagcgacccg
     5521 atcacccgcg agctctacct gaccacggtg gcggaacggg ccaaggtgag tcgcgacgtg
     5581 ctgcaggcgg agctggcgaa gcgggcggac aatcggatgg gcggacggtc ggatgacgcg
     5641 gggcgacacg ggccgggcgc cccatccgac cgaccgtccc cgcgcccctc tgacgctgca
     5701 tccagccgac cttccgaccg cccgaccccc cgccgccgct ccggcgacga gaagagcttc
     5761 ctgcgcgtgc tgttggcgct gccggagtgg cgggagcgcg cgcggcagga cgccgtcaag
     5821 cccgagtggt tcgagcggaa ggaataccgc gaggtcttcg cgcagttgct ggcgggggcg
     5881 gcgctcccgg acgcgctctc cgacgaggcg cagctggcgt ggtcggagct ccaggcgtcg
     5941 gcgcacggcc tgacggagaa tgcgggtgac gtgtacgagg ccgcgcggga ggcgctcgag
     6001 gcgcggccgc tgctccgcca gttcgacgcg ctgcaggcgc agatcgacgt ggccggaccc
     6061 gacgaactcg aggtgctcct ccgccgcaag gaggagatgc gcgccgacct gaagcgccgg
     6121 ttccccaacg aatggcagcg ccgtccgctc cgccgcgccg gtcggcgctg acgacggctt
     6181 tcgacccacg actcacgact cacgactcac gaccctcacc caaagcgacc ccaaatgacc
     6241 gacgaactcg tcaagctgct cgacctgcag gccaaggacc ttgccctcct cgagctcgac
     6301 gtccgctccc ttgagatcga cgaggaggag gcggccctcg aacgtgaagt ggccggcgcc
     6361 gtcggcaagg ccgcgcaggc cgagcgcgag gccaaggatg ccgccgcccg gcgcgatgaa
     6421 ctcgagcagc ggatcgagag ctacaagaag ctgaacgacc gccgccgcca gcggctggag
     6481 acggtccgga acccgaagga agcggcgacg ctgatggcgg aactcgacct ggcgcagtcg
     6541 gtggtgaagc gcgaggaagg ggagtgggtc aaggccgccg acgccgtcgc cctggccgag
     6601 gagcggtcca agctcgccgc ggcgcaggtg gccgagatgc gcggagcgca gtccgaggcg
     6661 cgcgacgcgg tggccgccaa gcggaaggcg ctggagggtg accgcgcggc agcgagtgcc
     6721 gcgcgggagg cggcggccaa gggcgtcgag aagccgctcc ggtcgcgcta cgaacggctc
     6781 ttcaactccc gcaagcggcc cgtggtggtg gcgctggtgg gggtgagctg cagcgcctgt
     6841 cgcaccgccg tgccgaccag ccgccgctca cccacctcgg gggggctcct ggccgacccc
     6901 tgcgagtcgt gcggcgtgat cctgtaccgg ttggcggtcg aagggtgatg gcccgccggc
     6961 gcgaccgcgt atcgtgatcc gatgacctcc gccgcgctcg tcacccgctt tccggtgccg
     7021 ccgctccgct gggtggtggc gccgcgggcc gagcccgcgc tcgcgcgcga gctcgccgcc
     7081 cagctgtcgg tgccgctgcc gctggccgag ctcctggtcc agcgcggctt tgccgaccgc
     7141 gactcggcgc ggaaatttct caagccggca cttgacggac tctcggatcc ctatgcgatg
     7201 gcggggatgc ggggggcggt ggatgcggtc gttcgcgcga tccacgcgca gcagaccatc
     7261 ctcgtccacg gcgactacga cgtcgacggc cagtgcgcca ccgcgctcct cacccgcgtg
     7321 ctccgcgccg ccggcgcccg ggtggtgccg ttcatccccc accgcatggg cgacggctac
     7381 gatttcggtc ccgcgggcgt caaggccgcc gcggacgccg gcgcgtcact catcatcacc
     7441 tgcgactgcg gcatcaccgc cgtcgacgcg gtgcgctcgg cgcgggccgc ggggcgcgag
     7501 gtcatcgtca ccgaccacca cctccccggt cccgcgctcc ccgatgccac
//
LOCUS       DASUAP010000356         3754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_271511, whole genome shotgun sequence.
ACCESSION   DASUAP010000356 DASUAP010000000
VERSION     DASUAP010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271511"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFU45_09610"
     CDS             <1..286
                     /locus_tag="VFU45_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359366.1"
                     /translation="IKHGDAIRPVRVRTGLTDLEYSEVITGLQEGDSVLVLPSASLVQ
                     SQQQFKERINRFTGGGSVPGMKATPTTTPKPAAGAAAGGGGGGGNRGGGR"
     gene            293..1504
                     /locus_tag="VFU45_09615"
     CDS             293..1504
                     /locus_tag="VFU45_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5359367.1"
                     /translation="MLIGETVTVAFQSIRANKLRAALTMLGIIIGVGAVITMVALGSG
                     AQKSVEDRINALGANLLTIFAGQSFMGGFASGNRVSLTTDDAAALDSASTTLAAVVPE
                     ISGNLQIKYADQNFNLSVTGTTPNYIQAHNYTMPYGQMFTRGDDDGRQRYAVLGASLP
                     EEWGQNPVSLLHQMIHIRGIPFEIIGILSPKGAQGFSNPDEQILIPLQTAKYRIFGSD
                     RLRSISVEIKDGVPPELAMVDIERVMRRKHKIAPGADDDFQIRNQSDILATQQQATQV
                     FTALLASIAAVSLIVGGIGIMNIMLVSVTERTKEIGIRKALGASRGTILFQFLIEALV
                     LCLTGGILGILLGVTGAVVLAKIFSWNTLISPVAIAIAFVFSGAVGLFFGLWPAKRAA
                     SLDPVEALRYE"
     gene            1716..>3754
                     /locus_tag="VFU45_09620"
     CDS             1716..>3754
                     /locus_tag="VFU45_09620"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5359368.1"
                     /translation="MLAVALLFQVSAALPAPVNGAGAPSVPIPRIDATATIDGRLDEA
                     PWQQAARLSGFWEYQPADGRPAGEETEVRVWYSSTAIYFGIIAHDPRPDAIRATVADR
                     DNIQGDDNVRIYLDTFNDHRRAYFFGVNPLGAQEDGVRAEGGFSAGSITGGTTDLNPD
                     FLWESKGHLTPDGYVVEIRIPFKSLRFRGADPKAWGIQVIRTVQRTGYQDAWTDLRRA
                     NASFLLQDGTLTGIHDIHRGVVVEAQPFVTGAVSGQQDSTGTFTRGAWRTEFGANLKL
                     GFSSFSVDATANPDFSQVESDAGQVTVNQRFALYYPEKRPFFLEGIELFSTPNQLVYT
                     RQIQNPVGGLKVAGKVGRFTIAHLTALDEPGGSSDNALFDVGRVRADFGENSTAGVTY
                     TDRTAGSLYSRVLAGDVRYVFGKLYYFESQLGGSWVHDSIGQRSSPIWHEEFDRTGRQ
                     WGFNYQLNGIGRGFEARAGFVPRTDIVTAHGFNRFTWYGAPGALMESFTVFFGPQATW
                     DYAHFSAAHPIEGSESANMMVRLRGGWNLSLVPTHAYVLPDPTLYTGYAVGHGANPTT
                     PYALPGMLDGWTGQLTAGTPVYQGFNASASLFRGAAVLFAEGSLGTETTVTGSLSLRP
                     SQQVRATGSLIVDRLTRDRTGEEFARTVIPRLEVDFQPRRSLFFRFVGEYRSQRLA"
BASE COUNT          617 a         1384 c         1193 g          560 t
ORIGIN      
        1 catcaagcac ggcgacgcca tccgcccggt gcgggtgcgg accgggctca ccgacctcga
       61 gtacagcgag gtgatcaccg ggctccagga aggggactcg gtcctcgtcc tgccgagcgc
      121 gagcctggtg cagagccagc agcagttcaa ggagcgcatc aaccggttca cgggcggcgg
      181 cagcgtcccc ggcatgaagg cgacgcctac caccacgccc aagccggcgg cgggggctgc
      241 ggccggtggt ggcggcggcg gcgggaaccg cggcggcgga cggtaacgac ccatgctcat
      301 cggcgaaacg gtcaccgtcg cgttccagtc gatccgggcc aacaagctcc gggccgccct
      361 cacgatgctg gggatcatca tcggcgtcgg cgccgtcatc acgatggtgg ccctcggctc
      421 cggcgcccag aagtcggtcg aggaccggat caacgcgctc ggcgccaacc tgctcacgat
      481 cttcgccggc cagtcgttca tgggaggctt cgcctcgggc aaccgggtga gcctcaccac
      541 cgacgacgcg gcggccctcg acagcgcctc caccacgctc gcggccgtgg tccccgagat
      601 ctccgggaac ctgcagatca agtacgccga ccagaacttc aacctgtcgg tcaccggcac
      661 gaccccgaac tacatccagg cccacaacta cacgatgccg tacgggcaga tgttcacacg
      721 gggcgatgac gacgggcgcc agcggtatgc cgtgctcggt gcctcgctgc cggaggagtg
      781 gggacagaac cccgtctcgc tcctgcacca gatgatccac atccgcggga tccccttcga
      841 gatcatcggg atcctgagcc ccaagggcgc gcagggattc agcaaccccg acgagcagat
      901 cctgatcccc ctccagactg ccaagtaccg gatcttcggc agcgaccggc tccgctccat
      961 ctcggtggag atcaaggacg gggtcccgcc cgaactcgcg atggtggaca tcgagcgggt
     1021 catgcgccgc aagcacaaga tcgcccccgg cgccgacgac gatttccaga tccggaacca
     1081 gtcggacatc ctggccaccc agcagcaggc cacccaggtc ttcaccgccc tgctggccag
     1141 catcgcggcg gtgagcctca tcgtgggcgg catcggcatc atgaacatca tgctggtgtc
     1201 ggtcacggaa cggaccaagg agatcggcat ccgcaaggcg ctcggcgcct cgcgggggac
     1261 gatcctcttc cagttcctca tcgaggcgct ggtgctctgc ctcaccggcg ggatcctcgg
     1321 catcctcctc ggcgtcaccg gggcggtggt cctggctaag atcttcagct ggaacacgct
     1381 catctcgccg gtcgcgatcg ccatcgcgtt cgtgttcagc ggcgcggtcg ggctcttctt
     1441 cgggctctgg cccgccaagc gcgccgcgag cctcgacccg gtggaggcgc tccggtacga
     1501 gtagcgggag aagagtcgag gagaagaaga ggtgaggaga agatgaggtg gggaggaggc
     1561 gaggcgagga attgaagtgg cgaacggggt cccgatcgac atcggagccc cgttcgcctc
     1621 atctccccgg ctcctcctcg cctcttcccc tcctctccgc atcttctcct cgtctcgcgc
     1681 ccgattacct tacctgacct tcctctccgg ccaacatgct cgctgtcgca ctgctcttcc
     1741 aggtgtccgc cgccctcccc gccccggtca acggcgcggg cgcgcccagc gtccccatcc
     1801 cccgcatcga cgccaccgcc accatcgacg gacggctcga cgaggcgccg tggcagcagg
     1861 ccgcccggct gtcggggttc tgggaatacc agcccgccga tggccggccc gcaggcgagg
     1921 agaccgaggt gcgggtctgg tactcctcca ccgccatcta cttcgggatc atcgcccacg
     1981 atccgaggcc cgacgccatc cgcgccaccg tggccgaccg cgacaacatc cagggcgacg
     2041 acaacgtccg gatctacctc gacaccttca acgaccatcg acgcgcctac ttcttcggcg
     2101 tcaacccgct cggcgcgcag gaggacggtg tccgcgccga aggcgggttc tccgccggga
     2161 gcatcacggg cggcaccacc gacctgaacc cggacttcct ctgggaatcg aagggccacc
     2221 tgacccccga cgggtacgtg gtcgagatcc ggatcccgtt caagagcctg cgctttcgcg
     2281 gcgccgaccc caaggcgtgg ggcatccagg tcatccgcac cgtccagcgc accgggtacc
     2341 aggacgcctg gaccgacctc cggcgcgcca acgcgagctt cctcctgcag gacggcaccc
     2401 tcaccggcat ccacgacatc caccgcggcg tggtggtcga ggcccagccg ttcgtgaccg
     2461 gcgcggtctc cggccagcag gacagcaccg gcacgttcac gcggggcgcg tggcgcaccg
     2521 agttcggcgc caacctcaag ctcggcttca gcagcttctc ggtcgacgcg acggccaacc
     2581 ccgacttcag ccaggtggag agcgacgccg gccaggtcac cgtcaaccag cggttcgcgc
     2641 tctactaccc ggaaaagcgc cccttcttcc tggaggggat cgaactcttc tccacgccca
     2701 accagctggt gtacacccgc cagatccaga atccggtggg cgggctcaag gtcgcaggca
     2761 aggtcgggcg cttcaccatc gcccacctga cggcgctgga cgagccgggc gggagctccg
     2821 acaatgcact cttcgacgtg gggcgggtcc gggccgattt cggggagaac tccaccgccg
     2881 gcgtcaccta caccgaccgg accgccggct cgctctacag ccgggtgctg gcgggcgatg
     2941 tgcgctacgt cttcggcaag ctctactact tcgagtcgca gctgggcggt tcctgggtgc
     3001 acgactcgat cgggcagcgg tcgagcccga tctggcacga ggaattcgac cgcaccggtc
     3061 gtcagtgggg attcaactac cagctgaacg gcatcggacg cggcttcgag gcgcgggcgg
     3121 ggttcgtgcc gcggaccgac atcgtgaccg cgcacggctt caaccgcttc acctggtacg
     3181 gcgcacccgg cgcgttgatg gagagcttca ccgtcttctt cggcccgcag gccacctggg
     3241 actacgccca cttctccgcc gcgcacccca tcgaggggag cgagagcgcc aacatgatgg
     3301 tccggctccg gggcggctgg aacctgagcc tcgtccctac ccacgcgtac gtgctccccg
     3361 acccgaccct ctacaccggg tacgccgtgg gccacggcgc caacccgacc accccgtacg
     3421 cccttccggg gatgctcgac ggatggaccg gccagctcac cgccggcacg ccggtgtacc
     3481 agggattcaa cgccagcgcc tcgctcttcc gcggcgccgc ggtgctcttt gccgaaggaa
     3541 gcctgggcac cgagacgacc gtcaccggat cgctgtcgct gcggccgagc cagcaggtcc
     3601 gggccaccgg cagcctgatc gtcgaccggc ttacccgcga ccgcaccggg gaggagttcg
     3661 cgcgaacggt catcccgcgc ctcgaggtcg atttccagcc ccggcgttcg ctcttcttcc
     3721 gcttcgtcgg cgagtaccgc tcccagcgcc tcgc
//
LOCUS       DASUAP010000357         6411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_720847, whole genome shotgun sequence.
ACCESSION   DASUAP010000357 DASUAP010000000
VERSION     DASUAP010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720847"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFU45_09625"
     CDS             complement(<1..511)
                     /locus_tag="VFU45_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5359369.1"
                     /translation="MTEWFEEWFGEEYLHLYPHRDDVDAERLVALLRRTLPWRDGWRV
                     LDFGCGAGRHARALAEAGARPVGLDLSLSLLRRARSVSSAPLVRADLRHPPVRPRSVD
                     LAVNLFTSFGYFDTDVEHASALAAMAGTVRPGGWFVLDFLEADTVRRSLVAHEAHGPD
                     ALITAVDRAL"
     gene            complement(512..979)
                     /gene="rlmH"
                     /locus_tag="VFU45_09630"
     CDS             complement(512..979)
                     /gene="rlmH"
                     /locus_tag="VFU45_09630"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU5359370.1"
                     /translation="MRLALVAVGALRGPGFRAACDEYLARLRRYAEVTEREVKEAGRV
                     RDVASRQAAEADRLAEALPVGSVVVALAREGTPWTSRALADRLDRWRLEARPVAFVVG
                     GSTGLAPAFIQRASARWSLGPLTLPHELARIVVVEQLYRAFTILRGEPYHKGR"
     gene            complement(979..2250)
                     /locus_tag="VFU45_09635"
     CDS             complement(979..2250)
                     /locus_tag="VFU45_09635"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5359371.1"
                     /translation="MRPEKDTFLHEENPFEAMMSRFDFAAQKLSLDKGLYRVLRNPEK
                     QIIVSVPVLRDNGEVEVYTGYRVLYNTSRGPAKGGIRFDLNVTLDEVKALAAWMTWKC
                     AVVNVPFGGAKGGVICDPSTMSNTELERLTRRYTSAIIDTLGPDSDVPAPDVNTNERV
                     MAWIMDTYSMHKRHTVTAVVTGKPIEMGGSLGRREATGRGCMLVTREALKRLKMPLKG
                     ARVAVQGFGNVGSITAQLLHEQGATIVAVSDKQGAIHNAKGLDITDVLAWVRDHRFLQ
                     GYPKGESLSNAELLRLDCDVLVPAALENVITSENAKDIRAKVICEGANGPTTAGADKI
                     LEEKGIFVIPDILANAGGVTVSYFEWVQDRGGYFWDLDTVNQRLERIMVRSFDEVVGM
                     ADRQKVNNRIAAYMLSIERVAAVHRLRGLYA"
     gene            complement(2315..3367)
                     /gene="lpxK"
                     /locus_tag="VFU45_09640"
     CDS             complement(2315..3367)
                     /gene="lpxK"
                     /locus_tag="VFU45_09640"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEU5359372.1"
                     /translation="MTARRGDAHRIIRWLWTSRRIDARLVRLALLPFSLAYRAVMIVR
                     AWCYHRGWFERHELPLPSVAVGNLTVGGSGKTPVAIWIARHYAGRGLVPGILLRGYGS
                     GDETLVHARAVPSGAVVADPDRVAGAALAVAKGAQVLVLDDAFQRLDVQRDFNVLVVS
                     AEATRAVRWTLPAGPWREGWEALRRADAVVVTRKRATAAAAAELAAALERQVAGPVAV
                     AHLGLTALEGLVSGRRLPAGVLNGQRVVAASAIGDPDAFVGQVKLTGAAVQVATWKDH
                     HAYRDEDVAWLAGAARKADHVVITEKDAVKLRDRWPAGAPEPLIAILDLAWERGGEEF
                     VSGLDAVVTPAEMLSL"
     gene            complement(3364..3993)
                     /locus_tag="VFU45_09645"
     CDS             complement(3364..3993)
                     /locus_tag="VFU45_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5359373.1"
                     /translation="MRIRIPPAVGAVLGPALVRGLGLTWRLRPEGADRWGAALESGRP
                     IVMMCWHESLLPLVWHHRGQGITLVVSEARDGGYLADLGSALGYRLVRGSSTRGGARA
                     LLGAVRELDEGHIVAFTPDGPQGPRRELKPGVVAAAQRSGALIVALHAIPNRAWRFRS
                     WDRFGLPKPFTRIAVRYAEPFAVAAGPAGLEEGMRQAAAALGELTRDDP"
     gene            complement(3990..5108)
                     /gene="lpxB"
                     /locus_tag="VFU45_09650"
     CDS             complement(3990..5108)
                     /gene="lpxB"
                     /locus_tag="VFU45_09650"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEU5359374.1"
                     /translation="MDHRPARIFLSAGEPSGDLHGARLVEALRARLPGAVIEAFGGPR
                     MAAAGAKVLYPMERYTAFGLAEVIGKIPAHLRLLRQVEAQLAGRMYDLVIPIDYPGFN
                     LRLATAARRHTVRVLYYIVPQLWAWHRSRINVLRRTVDLLAVILPFEERFYRDLGVDA
                     AYVGNPLLDRTWPSRASARDGLGLPPDSRVLGIFPGSRGQEVRALWPAFRDTARRMLD
                     EGRCTHAVVAATPAGQYPDPGPLLLHHGDPVPVFAAADAALAKSGTTTLECALADVPM
                     VMAYRVHPLTWIIGRRLTDVPWFSLVNLIAEREVVPEILQGRVTVDELASGVGPLLDP
                     ASPARAAQLAGLADVRRRLGGPGAAARVADAAARLLDR"
     gene            complement(5111..6091)
                     /locus_tag="VFU45_09655"
     CDS             complement(5111..6091)
                     /locus_tag="VFU45_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5359375.1"
                     /translation="MSGPVPVGVIGVGALGRHHARHLAQHPGARLVGVYDASPARAAE
                     IAAQVGTEAWSDVDALLGKVRGVSIAAPTSFHHELGMRALDRGVGVLMEKPITVTVAE
                     ADDLIARAIRAGVPLHVGQIERFNRAVRAIAPVVRRPRFIECERLAPFSLRGADVSVV
                     LDLMVHDLDLALHLTGGAEAVDVRADGGALLSPTIDLARARIEFAGGAVASITASRLA
                     RGRVRRTRIYQADGYLSLDLATGAAEFTRLRAGWTPGQSAQSVDDVVERIPLTAPEAD
                     ALGLEVGHFVDALGGRASGGVSGQEGRAALALARRVEDAVARAPIVPSAH"
     gene            complement(6088..>6411)
                     /locus_tag="VFU45_09660"
     CDS             complement(6088..>6411)
                     /locus_tag="VFU45_09660"
                     /inference="COORDINATES: protein motif:HMM:NF025097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HEU5359376.1"
                     /translation="LVAVHQFVTIGSFCFVGGCTRVNQDIPPYVKAVGNPMELYGLNT
                     IGLQRAGFPAETITALKRGYRLFFNSDLNLSQAVERARADLPQTAEVQRFIAFIESSE
                     RGVPA"
BASE COUNT          971 a         2356 c         2246 g          838 t
ORIGIN      
        1 agagggcgcg atccactgcc gtgatgagcg catccgggcc gtgtgcttcg tgggcgacca
       61 gcgagcggcg gacggtgtcc gcctccagga aatccagcac gaaccacccg ccgggtcgca
      121 ccgtcccggc catggcggcc agggcactgg cgtgctcgac gtcggtatca aaataaccga
      181 agctcgtgaa gagattgacc gcgaggtcga ccgatcgcgg acggacgggt gggtgccgca
      241 ggtcggcgcg aacgaggggg gcggaggaca ccgagcgcgc ccggcggagg aggctcagcg
      301 agaggtcgag gccgaccggg cgggcgccgg cctctgccag cgcccgggcg tggcgcccgg
      361 cgccgcaccc gaagtcgagg acccgccacc catcccgcca cggcagggtg cgacggagaa
      421 gggccaccag gcgctcggcg tcgacatcgt cccgatgggg atacaggtgc aggtactcct
      481 cgccgaacca ctcctcgaac cactcggtca tctaccgacc cttgtgatac ggctctcccc
      541 gcaggatggt gaacgcccgg tacagctgct ccaccaccac gatccgggcg agctcgtggg
      601 gcaacgtgag cggtcccagg ctccagcgcg ccgatgcgcg ctggatgaac gcaggtgcca
      661 ggcccgtgga cccgccgacc acgaacgcca ccggacgggc ctcgagccgc catcggtcca
      721 gccgatcggc gagcgcccgg ctggtccagg gcgttccctc ccgggcgagc gccaccacca
      781 cgctacccac cggcaaggcc tcggccagtc ggtcggcctc cgcggcctgg cgggacgcga
      841 cgtccctgac ccggcccgcc tccttcacct cccgctccgt gacctcggca taccggcgga
      901 gccgggcgag gtattcatcg cacgccgcgc ggaagccggg gccccgcagg gccccgacgg
      961 ccacgagcgc gaggcgcatc aggcgtacag gccgcgcagg cgatgcaccg ccgcaacccg
     1021 ttcgatcgac agcatgtacg cggcgatgcg gttgttcacc ttctggcgat cggccatgcc
     1081 caccacctca tcgaatgagc gcaccatgat gcgctcgagg cgctggttga cggtgtccag
     1141 gtcccagaag tagccgccgc gatcctgcac ccactcgaag tagctcacgg tgacgccgcc
     1201 ggcattggcc aggatgtccg ggatgacgaa gatccccttc tcctcgagga tcttgtcggc
     1261 gccggccgtg gtggggccat tggccccctc gcagatcacc ttggcgcgga tgtccttggc
     1321 gttctcgctg gtgatgacgt tctcgagcgc cgccgggacg agcacgtcgc aatcgagccg
     1381 gaggagctcc gcgttgctca gcgactcgcc cttcggatag ccctggagga agcggtggtc
     1441 gcggacccag gcgagcacgt cggtgatgtc gaggcccttg gcattgtgga tggccccctg
     1501 cttgtcgctc acggccacga tggtcgcgcc ctgctcgtgg agcaactgcg ccgtgatgga
     1561 ccccacgttc ccgaagccct ggacggccac ccgcgcgccc ttgagcggca tcttgagccg
     1621 cttgagcgct tcccgggtca ccagcatgca gccgcggccg gtggcctccc gccgcccgag
     1681 cgagccgccc atctcgatcg gcttgccggt caccaccgcg gtcacggtgt ggcgcttgtg
     1741 catggaatag gtgtccatga tccacgccat aactcgttcg ttggtgttga cgtccggggc
     1801 cggcacgtcc gaatcggggc cgagggtgtc gatgatggcc gaagtgtagc gccgggtcag
     1861 gcgctcgagc tcggtgttcg acatcgtgct cgggtcgcag atcacgccgc ccttggcgcc
     1921 gccgaacggc acgttgacga ccgcgcactt ccaggtcatc caggcggcga gcgcctttac
     1981 ttcgtccagc gtgacattca ggtcgaaccg gatgccgccc ttggccgggc cgcgcgaggt
     2041 gttgtagagc acccgatacc cggtgtagac ctcgacctcg ccgttgtcgc ggagcaccgg
     2101 taccgacacg atgatctgct tctcggggtt gcggagaacc cggtagagcc ccttgtcgag
     2161 gctcagcttc tgggcggcga agtcgaagcg ggacatcatc gcctcgaagg ggttctcctc
     2221 gtgaaggaag gtgtccttct cggggcgcat cacgttctgg gtcggacggg gacttgcggt
     2281 tgtcatacgg gttggcgcag ttctcctggc gcggtcaaag ggacaacatc tcggcgggcg
     2341 tcacgacggc gtcgaggccc gatacgaact cctccccgcc ccgctcccac gcgaggtcga
     2401 ggatggcgat caacggctcg ggggccccgg cgggccaccg gtcacgcagc ttcacggcat
     2461 ccttctcggt gatcacgacg tggtccgcct tgcgcgcggc gccggccagc cacgcgacgt
     2521 cctcgtcgcg gtatgcatga tggtccttcc aggtggcgac ctgcacggcg gcaccggtga
     2581 gcttgacctg gcccacgaac gcgtccggat caccgatggc ggacgccgcc acgacccgct
     2641 ggccgttgag gaccccggcc ggcagccggc ggccgctcac aagcccctcg agcgcggtga
     2701 gcccgaggtg ggcgaccgcc accggccccg ccacctgccg ttcgagcgcg gctgcgagct
     2761 ctgccgccgc cgcggccgtg gcacgcttgc gggtgaccac caccgcatcg gcccggcgga
     2821 gcgcctccca cccttcgcgc caggggccgg cgggcagcgt ccaccggacc gcacgggtcg
     2881 cctcggcgct caccacgagc acgttgaagt cgcgctgcac gtcgagccgc tggaacgcat
     2941 cgtccagcac gaggacctgg gcgcccttgg ccaccgcgag cgcggcaccg gccacgcggt
     3001 cgggatcggc cacgaccgct ccggacggga ccgcgcgggc gtggaccagc gtctcgtcgc
     3061 cgctcccata gccgcgaagg aggatccccg gcaccagtcc gcggccggcg tagtgccggg
     3121 cgatccagat ggcgaccggc gtcttgcccg acccgccgac cgtcagattg ccgaccgcga
     3181 ccgacggcag cggaagctcg tggcgctcga accagccccg atggtagcac cacgcccgca
     3241 cgatcatcac ggcccggtac gccaaggaga acggcagcag cgccaaccgg accagacgcg
     3301 cgtcgatccg acggctcgtc cagagccagc ggatgatccg gtgtgcgtcg ccccgccgcg
     3361 cggtcatggg tcgtccctgg taagttcgcc caacgcggcc gccgcctgcc gcatcccctc
     3421 ctccagcccc gccggccccg ccgccaccgc gaacggctcg gcataccgca ctgcgatccg
     3481 ggtgaacggc ttcggcagcc caaagcggtc ccacgaacgg aaccgccatg cccggttggg
     3541 gatcgcgtgg agcgccacga tgagcgcccc gctgcgctgc gcggcggcca ccacgccggg
     3601 cttgagttcc cgccgcggcc cctgcggccc atcgggcgtg aacgccacga tgtggccctc
     3661 atcgagctcc cggaccgcgc caaggagggc cctggcaccc ccgcgagtgc tcgaaccacg
     3721 gaccaggcgg tagccgagtg ccgaccccag atcggccagg tatccgccgt cgcgcgcctc
     3781 actcaccacc agggtgatcc cctgtccccg atggtgccag accagcggca ggagcgactc
     3841 gtgccagcac atcatcacga tcggccgccc tgactcgagc gccgcccccc accggtccgc
     3901 cccctccgga cgcaaccgcc aggtgaggcc aagtccgcgg accagcgccg gcccgagcac
     3961 cgcaccgacc gccggcggga tccggatcct catcggtcga gcagccgggc cgcggcatcg
     4021 gccacccggg ccgccgcgcc cgggccgcca agccggcggc ggacgtcggc gagtcctgcg
     4081 agctgtgccg cccgggccgg cgacgccggg tcgaggagcg gccccactcc ggacgcgagt
     4141 tcgtcgacgg tgacccggcc ctggaggatc tccggcacca cctcgcgctc ggcgatcagg
     4201 ttcaccaggc tgaaccacgg cacgtcggtc aggcgccgcc cgatgatcca cgtgagcggg
     4261 tgcacgcggt acgccatgac catcggcacg tcggccagcg cgcattcgag cgtcgtcgtc
     4321 cccgacttgg cgagcgctgc gtcggccgcc gcaaagacag gcaccgggtc gccgtggtgc
     4381 aggagcagcg gacccggatc gggatactgg cccgccggtg tcgcggccac gacggcgtgg
     4441 gtgcagcgcc cctcgtccag catccgccgc gccgtgtcgc ggaaggcggg ccagagcgcc
     4501 cggacctcct gccccctgct ccccggaaag atacccaaga cccgggaatc gggggggagc
     4561 ccgagtccat cccgggccga cgcgcgggag ggccaggtcc ggtcgagcag cggattgccc
     4621 acgtacgctg catcgacgcc gaggtcccgg tagaaccgct cctcgaacgg gaggatgacg
     4681 gccagcaggt cgaccgtgcg ccgcagcacg ttgatgcggg agcggtgcca ggcccagagc
     4741 tgggggacga tgtaatagag gacccggacc gtgtgccgac gcgcggcggt ggccaggcgc
     4801 aggttgaagc cgggataatc gatggggatg acgagatcgt acatccggcc ggcgagttgc
     4861 gcctccacct ggcggaggag ccggaggtgg gcggggatct tgccgatcac ctcggccagg
     4921 ccgaacgcgg tataccgctc catcgggtag agcaccttgg ccccggcggc ggccatccgc
     4981 ggcccgccga acgcctcgat cacggcaccg ggcagcctgg cgcgcagggc ctccaccagg
     5041 cgggcgccat ggaggtcgcc cgaaggttcg cccgccgaca ggaagatccg ggccgggcgg
     5101 tggtccatcg ctagtgcgcc gagggaacga tcggggcgcg ggccacggcg tcctcgaccc
     5161 ggcgggccag tgccagcgcg gctcggcctt cctggcccga gacgcctccc gatgcccgcc
     5221 cccccagcgc atcgacgaag tgcccgacct ccagtcccag ggcatcggcc tcgggcgccg
     5281 tgagcgggat gcgctccacc acgtcatcca ccgactgcgc cgattggccg ggcgtccacc
     5341 ctgcgcgaag gcgggtgaac tcggccgccc cggtggcgag gtcgaggctc aggtacccgt
     5401 cggcctggta gatgcgcgtc cggcgcaccc gaccccgcgc gaggcgcgag gcggtgatgg
     5461 acgcgaccgc cccgccggcg aactcgatcc gcgcccgggc caggtcgatg gtgggcgaca
     5521 gcaacgcgcc gccgtcggcg cgcacatcca ccgcctcggc gccgcccgtc aggtgcagcg
     5581 ccaggtccag gtcgtgcacc atgaggtcga gcacgaccga gacatcggca ccgcgcaggc
     5641 tgaacggggc caggcgctcg cactcgatga accgggggcg gcggaccacc ggcgcaatgg
     5701 cgcggaccgc acgattgaag cgctcgatct gtcccacgtg gagcggcacg ccggcccgga
     5761 tcgcgcgggc aatgaggtcg tcggcctccg cgacggtcac cgtgatcggc ttttccatca
     5821 gcacgccgac gccgcggtcg agggcgcgca tgcccagctc atgatggaag gaggtcgggg
     5881 cggcgatgga cacgcctcgg accttgccga ggagcgcatc gacgtcgctc caggcctccg
     5941 tccccacctg cgccgcgatc tcggccgccc gcgccggcga cgcgtcgtag acgcccacca
     6001 ggcgggcgcc ggggtgctgg gccaggtgcc gggcgtgatg gcggcccagg gcgcccaccc
     6061 cgatcacccc gacggggacc ggtccgctca cgccgggacg ccccgctccg aggactcgat
     6121 gaaggcgatg aaccgctgca cttcggcggt ctgcggcagg tcggcgcggg cgcgctcgac
     6181 cgcctgcgac aggttgaggt ccgaattgaa gaagagccgg tagccgcgct tcagcgccgt
     6241 gatggtctcg gccgggaacc cggcacgctg gagtccgatc gtgttgagac catagagctc
     6301 catcgggttg ccgaccgcct tcacgtaggg cgggatgtcc tggttgacgc gcgtgcagcc
     6361 gcccacgaag cagaagctcc cgatggtgac gaactggtgc accgccacca g
//
LOCUS       DASUAP010000358         2937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1061935, whole genome shotgun sequence.
ACCESSION   DASUAP010000358 DASUAP010000000
VERSION     DASUAP010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061935"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VFU45_09665"
     CDS             <1..1235
                     /locus_tag="VFU45_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU5359377.1"
                     /translation="AAAGLSAAFGAPIAGVLFVLEKLVGSFRTSVLAPIVVASVSAAA
                     VTRVGLGRDQVIRIPAEYHVGGARELVLYAAVGLLGGAIGALYARAAWKVPDWLRPLP
                     QWLRILVASVLVAVVSTQFSPALWGHGHQGLDLALVADSTALVLFALCLAKIAATALT
                     LGGGGVGGVFTPALVVGGTFGAAAGVALAHLIPGSNIQPVAFALVGMAAVVAAATHAP
                     LTAVFMVLEMSGDYGLILPILLGGVLAFVVARSLYKESIYTEWLARRGEHISHGTDES
                     VLAALTVADAYRADALVLPATATLGEVLADVRQSGQLEFPVVDAANTVVGMLTWEDLK
                     RALADGTRDALVSELAEPPRDGVRLDDDLRTALRVLRERDAQLLPVLDPAAPHHLRGV
                     IGRSEIFSAYERAVEASE"
     gene            complement(1547..2314)
                     /locus_tag="VFU45_09670"
     CDS             complement(1547..2314)
                     /locus_tag="VFU45_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU5359378.1"
                     /translation="MAVIAAWLESRTALGALRRSLPRGSGGLLVCRSRAAIDRALRSR
                     LVDAVVFAPRTMTLAEVAELAAEFPGVPAFAYASFRPDDGELLASYARRGVPLLVEGV
                     DDAVAGELVARGSAQVDRRRALADAPRLLRLRSPLQREVWTLLLGMADRPLRTAELAR
                     RLRVTREHLSRQFAVEAAPNLKRVIDLNRVAAAAHLLGNPGHTTARVAAILHFASASH
                     LDRTSRRITGVGAARLGQLGPRGALEAFARGKTRSRV"
     gene            complement(2317..2841)
                     /locus_tag="VFU45_09675"
     CDS             complement(2317..2841)
                     /locus_tag="VFU45_09675"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5359379.1"
                     /translation="MTTHKRRPDREVSAGGIVFRRFADGFRFLLINDIYRNWGFPKGH
                     LEEGETPAEAALRETREETGLDDLVLQGPIRIIDWHFRFKGRPVHKFCHFFLLESPAA
                     EPRPQVAEGITDARWHPLDEALKTLSYENARGVLRRAGEMARTLVATGPGRPTPRPAP
                     APLPAAVPVPPTGD"
BASE COUNT          355 a         1074 c         1020 g          488 t
ORIGIN      
        1 gcgccgccgc cgggctctcc gccgcgttcg gcgcgccgat cgcgggcgtg ctcttcgtgc
       61 tcgaaaagct ggtcggaagc ttccggacct cggtgctggc gccgatcgtg gtagcgagcg
      121 tctccgccgc cgccgtcacc cgcgtcggcc tcggccggga ccaggtcatc cgcatccccg
      181 ccgagtacca cgtgggcggc gcccgcgagc tggtgctgta cgcggcggtc ggcctgctgg
      241 gcggtgccat cggcgccctg tatgcccgcg ccgcgtggaa ggtgcccgac tggctccgcc
      301 cgctgccgca gtggctccgg atactggtgg cctcggtgct cgtggcggtc gtgtccaccc
      361 agttctcgcc ggcgctctgg ggccacgggc accaggggct cgacctggcg ctcgtggccg
      421 acagcaccgc gctcgtgctc ttcgccctct gcctcgccaa gatcgcggcc accgcgctca
      481 ccctgggcgg cggcggcgtg ggcggcgtct tcacgcctgc gctcgtcgtg ggtggcacct
      541 tcggcgccgc cgccggcgtg gcgctggccc acctgatccc cggctcgaac atccagccgg
      601 tggcgttcgc gctcgtcggg atggcggcgg tcgtcgcggc cgccacccac gcgccgctca
      661 cggccgtgtt catggtgctc gagatgagcg gtgactacgg cctcatcctg cccatccttc
      721 tcggcggcgt gctggcattc gtcgtggccc ggtcgctcta caaggagtcg atctacaccg
      781 aatggctggc ccgccgcggc gagcacattt cccacggcac tgacgagtcg gtgctggcgg
      841 ccctcaccgt cgccgacgcc taccgcgccg atgcgctcgt gcttcccgcc accgccacgc
      901 tgggggaggt gctcgccgac gtgcgccagt cgggacagct ggagttcccg gtggtcgacg
      961 ccgccaacac cgtcgtcggg atgctcacct gggaggacct caagcgcgcc ctggccgacg
     1021 ggacccgcga cgcgctggtg tcggagctcg ccgaaccgcc gcgcgacggc gtccgcctgg
     1081 acgacgacct gcgcaccgcc ctccgcgtcc tgcgcgagcg cgatgcccag ctcctgccgg
     1141 tcctcgaccc ggccgccccc caccacctgc gcggcgtcat cggccggagc gagatcttct
     1201 ccgcatacga gcgcgcggtg gaggcgagcg agtaagaagt gagaagtgag aagtgagaag
     1261 gacaaagtga gaaggggcct cggtgtttcg accgggcccc atttcacttc ctaccccctg
     1321 ccgccctgct cttccctgtt ccctgttccc ttcttcactc tgtcctgtcc cttctcactt
     1381 cttgttcctt ccttctccct gcctagttcc atcttccctc ttccctctcc cttttcaccc
     1441 ttcgctcttc acatatcact ctttcctttt cactcctcgg tcctcccttc ttacttctct
     1501 cttcttactt ctcccttctt acttctcact tctcacttct tacttcctac accctgctcc
     1561 gcgttttccc gcgggcgaac gcctcgaggg cgccgcgggg gccgagttgc cccaggcgcg
     1621 cggccccgac gccggtgatg cggcggctgg tacggtcgag gtggctggcc gacgcgaagt
     1681 gcaggatcgc cgcgacgcgg gcggtggtgt ggccgggatt gccgaggagg tgcgccgccg
     1741 ccgccacgcg gttgaggtcg atgacgcgct tgaggttggg cgcggcctcc acggcgaact
     1801 gccgcgacaa gtgctcccgt gtcacgcgga ggcggcgggc gagctccgcg gtccggagcg
     1861 ggcgatccgc catcccgagg agcagggtcc agacctcgcg ctgcagcggc gaccggagcc
     1921 gcagcagtcg cggcgcgtcg gccagggcgc gccggcggtc gacctgggcc gatccgcggg
     1981 ccacgagctc ccccgccacc gcgtcgtcca ccccttccac cagtaacggc accccccgcc
     2041 gggcgtagct cgcgagaagc tccccgtcat cggggcggaa cgaagcgtag gcgaacgccg
     2101 gcacgccggg gaactccgcc gcgagctcgg ccacctcggc gagcgtcatc gtgcggggcg
     2161 cgaacaccac ggcatcgacc agccggctcc ggagggcccg gtcgatcgcg gcccgggacc
     2221 ggcagaccag gaggcccccc gaaccgcggg ggagggaacg gcggagcgcg ccgagcgcgg
     2281 tgcggctctc gagccaggcg gcgatgaccg ccacggtcag tcgccggtcg gcgggacggg
     2341 aacggcggcg gggagcgggg cgggcgcggg ccgcggtgtg gggcgcccgg gtccggtggc
     2401 cacgagggtg cgggccattt cacccgcgcg ccggagcacc ccgcgggcgt tctcgtagct
     2461 cagcgtcttg agcgcctcgt cgagcggatg ccaacgcgca tcggtgatcc cctccgccac
     2521 ctgggggcgc ggctccgccg ccggtgattc gagcaggaag aagtggcaga acttgtggac
     2581 cggccgcccc ttgaaccgga agtgccagtc gatgatccgg atcgggccct ggaggaccag
     2641 gtcgtcgagc ccggtctcct cgcgcgtctc gcggagtgcg gcctcggcgg gggtctcccc
     2701 ctcctcgagg tggcccttgg ggaagcccca gttccggtag atgtcgttga tgagcaggaa
     2761 ccggaacccg tcggcgaacc gccggaacac gatgccgccg gccgagactt cgcgatcggg
     2821 gcggcgcttg tgggtagtca ttggtcgttg gtcattggtc gttggtcatt ggccaaggtg
     2881 gccaggcgtg agtgccgttt gcggagagcg acgtgctgtt gaccagtgac cggtgac
//
LOCUS       DASUAP010000359         5546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_447803, whole genome shotgun sequence.
ACCESSION   DASUAP010000359 DASUAP010000000
VERSION     DASUAP010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447803"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFU45_09680"
     CDS             complement(<1..652)
                     /locus_tag="VFU45_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5359380.1"
                     /translation="MTKPTAAADWLLIAVPGIIWGASFLFIAEGLEAVGPNGVTFVRI
                     LVGFLTLACFPAAWKEVPRSAWMAIAALGVLWLAFPLSMFPYAEERVSSALAGMLNGG
                     TSLTATIVAAVLARQLPARGVVQGLLIGIVGVVLVALPAVGQGSSSTLGVGMILVALV
                     SYGFALNLARPLQMRHGAIPVIWRAQMVAVVLTAPMGLHDLRHAHWTLAPLLSLLAL"
     gene            1073..1510
                     /gene="tnpA"
                     /locus_tag="VFU45_09685"
     CDS             1073..1510
                     /gene="tnpA"
                     /locus_tag="VFU45_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEU5359381.1"
                     /translation="MRHRILLHIVWTTRLRAPLIDADGARFLCTTFRALAREHRAAIL
                     EIGMVSTHVHLLIRVHPLTNISTFVARMKGVSSRVAKREAICSITWGDGYNVESVSLG
                     DEQKLRHYLRAQPYRHPEEAVPDWTGDVIGRETEAAHGLRSGA"
     gene            1583..1765
                     /locus_tag="VFU45_09690"
     CDS             1583..1765
                     /locus_tag="VFU45_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359382.1"
                     /translation="MPHPRHHAPIAWADGYNIESVGPGDEERLRHYLRAQPYRHPAEA
                     IDGWHGDPFALPKTRV"
     gene            1921..2421
                     /locus_tag="VFU45_09695"
     CDS             1921..2421
                     /locus_tag="VFU45_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5359383.1"
                     /translation="MPTGATVTIRPAVPADLPAIGRLGALLVQTHHDFDPARFIAASP
                     ATPQGYANFLGTQLTEARVVILVAEQEGNVAGYVYAGLKGMDYMALRGPAGAIYDVVV
                     DPAVRRRGVGRLLLDAAVERLKGMGAPQVVLSTAAKNEEAQRLFAKAGFRPTMVEMTK
                     GLGGGQ"
     gene            2919..3272
                     /locus_tag="VFU45_09700"
     CDS             2919..3272
                     /locus_tag="VFU45_09700"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5359384.1"
                     /translation="MQYIFLLYAQESGYGTLTEAEKAAWWTKINAYNDSLVKAGHYKM
                     TSGLQSPSTAKTLTKVRGALETTDGPFAETKEQLLGFYIIEAKGMDEACALAARAPMA
                     EFGKVEVRALNTRGE"
     gene            3275..3613
                     /locus_tag="VFU45_09705"
     CDS             3275..3613
                     /locus_tag="VFU45_09705"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5359385.1"
                     /translation="MRYLILIASNAKTWANMPTEELARVQAEYRDYGEAIKRSGHFVA
                     GSQLPRDKATTIRQRNGKLEMVDGPFAETKEQLGGFYLIEVKDHDEAMAVAGRCPGAK
                     YGCLDVRPTI"
     gene            3703..4062
                     /locus_tag="VFU45_09710"
     CDS             3703..4062
                     /locus_tag="VFU45_09710"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5359386.1"
                     /translation="MQYLLLIYFNEAEWEALPPAEKTRRREEFDAYSIKTSGSGHLVG
                     GAPLHPVRTATTLRSSKGRLEMVDGPFAETKEQLAGYYLMEAKDLDEALALARECPGS
                     KYATVEVRPCAPMGPRA"
     gene            4059..5309
                     /locus_tag="VFU45_09715"
     CDS             4059..5309
                     /locus_tag="VFU45_09715"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5359387.1"
                     /translation="MTEGARVREQLDQVFRADSRRVLATLIRLLGDFDRAEEALQEAF
                     VAAVEKWPATGIPANPYAWLVSAGRFKGIDRMRRDSRFEDESAISYPLSAAPSLPKDL
                     GEEAGIVDDRLRLIFTCCHPALAPEARVALTLRVVCGLSTAEIARAFLISEASLAQRL
                     HRAKEKIRDAGIPYEVPEATALPERLQTVLAVIYLVFNEGYAATAGEALIRSELCQEA
                     IRLGRLLVELLPDAPEAGALLGLMLLHHSRRDARTTPTGDLVLLDEQDRSLWDRGQIS
                     EGVRRVEEALRSGLAGPYAVEGAIAALHAQAPSSEQTDWRQIAALYQYLLRYRPGPVV
                     ELNHAVAVAMVDGPEEGLRLVDSIKARGGLDGYRWLPVARGELLRRSGRNPEAAAEFR
                     TALEMTSVDGERRLLLRRLEEVGG"
BASE COUNT          919 a         1911 c         1913 g          803 t
ORIGIN      
        1 ccagggccag gagcgagagg agcggcgcca gggtccagtg ggcgtgacga aggtcgtgga
       61 ggcccatcgg cgccgtgagc accaccgcca ccatctgggc gcgccagatg acgggaatgg
      121 cgccgtgccg catctggagc ggccgggcca ggttgagcgc gaagccgtac gacacgaggg
      181 caacgaggat catcccgacg cccagcgtgc tgctcgatcc ctgcccgacc gccggcagcg
      241 ccaccaggac cacccccacg atcccaatca ggagcccctg caccaccccg cgcgccggaa
      301 gctggcgcgc caggacggcg gccacgatcg tggcggtgag cgacgtcccg ccgttcagca
      361 tccccgcgag cgccgaactc acgcgttcct cggcgtaggg gaacatcgag agggggaacg
      421 ccagccagag gacgccgagc gccgcgatgg ccatccacgc cgaccgcggc acctccttcc
      481 acgcggcggg aaagcacgcc agcgtcagga atcccaccag gatccggacg aaggtgacgc
      541 cgttgggacc gacggcctcg agcccctcgg cgatgaacag gaacgaggcg ccccagatga
      601 tcccggggac ggcgatcagg agccagtcag cagcggcagt gggtttcgtc atggcgggaa
      661 ttgtacggcg gagcgcgtcg tcgggcgacc cagttcttgc tgtgatcggt gcgcgggggg
      721 attgcgcggc gtccgcgcgc ccgtttctcg gcgcctgaag gcgcctctcg gcaatgcgcc
      781 ctgaagggct ttccccgcgt tgggttgatt atcacagatc gggacgcgtc gagtatcgtc
      841 gtgtcggcaa cactggccct actttcacag agttttggcg acccgcgtgc aggcgcccgt
      901 ttctcggcgc ctgaaggcgc ctctcggcaa tgcgccctga agggcttctg ctccatctcc
      961 ctgtcagcgc ccgggcgcca gtccgatagc ggtctacagg gtctgcgcgc gaggggccct
     1021 cgatgcgcgg aagcggttac cgcgctatgg gcctcccgtc gtaccgtcgg gcatgcgcca
     1081 ccggatcctg ctgcacatcg tctggaccac ccgccttcgc gcaccgctga tcgacgcaga
     1141 cggcgcgcga tttctctgca cgacatttcg cgcgctcgcc cgcgagcatc gcgcggcgat
     1201 ccttgagatc gggatggtgt cgacccacgt ccatctgctg atcagggtcc acccgctgac
     1261 caacatttcg acgttcgtcg cgcgaatgaa gggtgtgtcg tcccgcgtgg cgaagcgtga
     1321 agcgatctgc tcgatcacct ggggcgacgg ctacaatgtc gagagtgtca gtctgggaga
     1381 cgagcagaag ttgcgccatt acctgcgagc gcagccgtac cggcatcccg aggaggccgt
     1441 tcccgattgg actggtgatg tgatcggccg cgagaccgaa gccgcacatg gactgaggag
     1501 cggcgcctga tgggtccacg gaacccaagc ccttcaaggc gcattgccga gaggcgcctt
     1561 caggcgccgg gaaatcgcgc gggtgcccca cccgcgccat cacgcgccga tcgcctgggc
     1621 cgacggctac aacatcgaga gcgtcggtcc cggcgacgaa gaacgactgc gccactacct
     1681 gcgggcacaa ccgtatcgcc atccggccga ggcgatcgac ggctggcacg gcgacccgtt
     1741 cgcgctgccg aagacccggg tgtaacgaat cggtgatctc gcctcggcgc gctcgccccg
     1801 gcgggtgatc ccgccggggc gcgggcgaca ggcagcggag gacaccccac ggcggcccgc
     1861 cgacgggccg ccttattttg taagcgccac cacgccatac cttcgggcaa ggagccttcc
     1921 gtgccgaccg gcgccaccgt caccatccgc ccggcagtcc ccgccgacct ccccgccatc
     1981 ggccggctcg gcgccctcct ggtccagacc caccacgatt tcgacccggc ccgcttcatt
     2041 gccgcgtccc ccgccacgcc acaggggtac gccaacttcc tcggcaccca gctcacggaa
     2101 gcgcgggtcg tcatcctggt ggccgagcag gagggaaacg tggcggggta cgtgtatgcg
     2161 gggctcaagg ggatggacta tatggcgctc cgcgggcccg cgggcgccat ctacgacgtg
     2221 gtggtcgatc cggcggtgcg gcgccggggc gtgggaagac tgctcctgga tgcggcggtc
     2281 gagcgactca aggggatggg cgctccgcag gtcgtcctct cgaccgcggc gaagaacgag
     2341 gaggcccagc ggctctttgc caaggcggga ttccgcccga cgatggtcga gatgaccaag
     2401 gggcttggcg gcgggcaatg acgtgggggt cgcctgcggc gacccgacgc tcggccaccc
     2461 catcgacccc gatgccggca tcactgccgc cccccggcgc ctgaaggcgc ctctcggcag
     2521 tgcgccgtaa acggctttgg aagtcggcag cctgccaagc cgggagccaa tgccctttag
     2581 ggcgcgtcgc cgagaggcgg cttcagccgc cggtgcctgc ggggcccgcg gacaaccgcg
     2641 tgccggcatc gcgaacgccc cccggcgcct gatccttcgc ttcgctcagg acaggcagcg
     2701 cctctctgga tgacgccgtg aacggctggg attcggaacg aaggccggcg ccccagcggc
     2761 accccccagc ccgtctcggc gggttgccga tccttcgcac cgctcaggac ggggaggcgc
     2821 acttacgcac ccacccctgc cctgtcattc cgcccagcgc ccgttcgact accgggtaca
     2881 gccgagcagt tccacattcc agcgcccgga ggcaccgaat gcaatacatc ttcctgctct
     2941 acgcccagga atcgggctac ggcaccctga ccgaggccga aaaggccgcc tggtggacca
     3001 agatcaacgc ctacaacgat tcactcgtga aggcgggtca ctacaagatg accagcgggc
     3061 tgcagtcgcc gagcaccgcg aagaccctca ccaaggtgcg gggcgccctc gagacgaccg
     3121 atggcccctt tgccgagacc aaggagcagc tcctcggttt ctacatcatc gaggcgaagg
     3181 gaatggatga agcctgtgcc ctcgcggcca gggcgccaat ggcggaattc ggcaaggtcg
     3241 aagtccgcgc actcaacacc cgtggggaat gaccatgcgc tacctcattc tcattgccag
     3301 caatgccaag acgtgggcga acatgcccac ggaagaactt gccagggtcc aggccgagta
     3361 ccgcgactat ggtgaagcga tcaagcggag cgggcacttt gtggcgggaa gccagctccc
     3421 tcgggacaag gcaacgacca tccgtcagcg caacgggaag ctcgaaatgg tcgacggccc
     3481 cttcgccgag acgaaggagc aactcggcgg cttctacctg atagaggtga aggaccacga
     3541 cgaggccatg gccgtcgcgg gacggtgccc gggagcgaaa tacggctgcc tcgacgtccg
     3601 gccgacgatc tagcatccgg ctcgagtgtc gttcctccct gccccggttc gaccaatggg
     3661 tgaaggctcg aggtcgcatt cacccacccc atggagacgt cgatgcaata cctgctgctg
     3721 atctacttca acgaagcgga atgggaggcc ctcccccccg ccgaaaagac ccggcgccgg
     3781 gaagagttcg acgcgtacag catcaagacc tccgggtcag ggcacctggt gggcggggcc
     3841 ccgctccacc cggtgcgcac cgctaccacg ctccggagca gcaaggggcg gctcgaaatg
     3901 gtcgacggcc cctttgccga gaccaaggag cagctggccg gctactacct gatggaggcg
     3961 aaggacctcg atgaggcgct cgccctcgcg agggagtgcc ccggcagcaa gtacgccacc
     4021 gtcgaagtcc gtccctgcgc cccgatgggt cccagggcgt gaccgaaggc gcgagggtcc
     4081 gggagcagct cgaccaggtc tttcgggccg actcccggcg cgtcctcgcc accctgatcc
     4141 gcctcctcgg cgatttcgat cgtgccgagg aggcgcttca ggaggcattc gtggcggctg
     4201 tcgagaagtg gccggccacg gggatcccgg cgaacccgta cgcgtggttg gtgagcgcgg
     4261 gccggttcaa ggggatcgat cggatgcggc gggacagcag gttcgaggat gaaagcgcta
     4321 tcagctatcc gctatccgct gctccgtcgc tgcccaagga tcttggcgaa gaggcgggca
     4381 tcgtcgatga ccgtctgcgg ctcatcttca cctgctgcca ccctgccctg gcgccggaag
     4441 cgcgggtggc gctcacactc cgggtggtct gcgggctctc gaccgcggag atcgcgcggg
     4501 cattcctcat ttcggaggca agcctggccc agcggctcca ccgcgccaag gagaagatcc
     4561 gcgacgcggg gatcccgtac gaggtccccg aggccacggc gctccccgaa cggctgcaga
     4621 cggtgctggc ggtcatctac ctcgtcttca acgaggggta cgccgccacc gccggcgagg
     4681 cgctgatccg ctcagagctc tgccaggagg cgatccgcct ggggcgcctc ctggtcgagc
     4741 tcctgcccga cgcacccgag gccggcgccc tcctcggcct gatgctcctg caccactccc
     4801 gccgcgacgc ccgcaccact cccaccggcg acctggtgct cctcgacgag caggaccgct
     4861 cgctctggga ccgcggccag atctcagagg gggtccgccg ggtcgaggag gcgctgcgtt
     4921 cggggctggc cgggccctac gccgtcgagg gcgccatcgc ggcactgcac gcccaagcgc
     4981 cgtcgtcgga gcagaccgac tggcgccaga tcgcggcgct gtatcagtat ctgcttcgct
     5041 accggccggg acccgtggtg gagctcaacc acgcggtggc ggtggcgatg gtggacggcc
     5101 ccgaagaagg gcttcggcta gtggattcga tcaaggcgcg cggcgggctc gacggctatc
     5161 gctggctgcc ggtggcgcgg ggggagcttc tgcggcgctc gggccggaac ccggaggcgg
     5221 cggcggagtt caggacggcg ctggagatga ccagtgtcga tggggagcgg cgattgctgc
     5281 tgcggcggct ggaggaggtc ggggggtagc ggagtacgtg ggggtcgcct gcggcgaccc
     5341 ggccgtggat tgccgccgtg gcctgtaggg ggtggcctgc ggccacccca acaacccgac
     5401 gcgagcatcg tgcccgtctc ctcggcgatg cgccgtaaac ggcttggggg gcggaggccg
     5461 atcgtgtgtg ggggccaaag cccttcaggg cgcattgccg agaggcggct tcagccgccg
     5521 ggcctgcggc gacccgacgc ccggcc
//
LOCUS       DASUAP010000360         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_625311, whole genome shotgun sequence.
ACCESSION   DASUAP010000360 DASUAP010000000
VERSION     DASUAP010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625311"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFU45_09720"
     CDS             <1..413
                     /locus_tag="VFU45_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359388.1"
                     /translation="DLTVVLVHGAEGRAAERDRALALRLQDEVAQRGRRAVVVSDAVY
                     DEHPALHPSAVIAIGGPGVNRLSAELLPSLPAVWAQDDKIFVQAARDATPPQAVVWGM
                     DADATAEAVEAFVFHGELDRLLGGLWRSPRKSLT"
     gene            466..2199
                     /locus_tag="VFU45_09725"
     CDS             466..2199
                     /locus_tag="VFU45_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU5359389.1"
                     /translation="MRFRALFALALLSLATCTNDLPVAPGGAGGGDLAIRPIFPASFA
                     AFAQALSIDAAKVAATRPGDTVVVFQQSVTFNPNDSVLALSVKVPFTADTATFDVTLT
                     FLSKAGVPLFQSVFPSLTLSRSAPVSPVDFSVAYIGPGDSTVAVSVSPNVDTVNADTT
                     LQFSISAIDVSQAPVTQLYVHWTSSNPAAPIDATGKLKGPGVAGQTVVHALVPSLYGT
                     IQDSAVITFVGAPPPAAGSFHGSVVDGATQLGIAGATVQASLAGAGTTYTATSAADGS
                     FSLGPLPAGAYDISLSASSYTGTKYLSATMPAGTGLLVPLPPVPLATLTGKPGALSGT
                     VLDAQTGAALPGATVLLEAYANAQGTTPVASTTADTTGAFSFTAQPEGTYTLEVSDSG
                     YVTATRTALVYGSTTTSGQNVVMSKGGLGFRVVLTWDSLPPDLDLHGIVPDSLGALSE
                     IYYANPGDTTTSHLDYDVTSGYGPETITIYKEFPGTYTFGVINYSAGTGLTPDTTVTT
                     LARSGAIVRVYDNNALLATYPVPNQAGTRWKVFSWNGSALTTINQMSYDTAGTGTFTV
                     ARPGAHLLKKR"
BASE COUNT          322 a          809 c          752 g          322 t
ORIGIN      
        1 ccgacctgac cgtggtgctg gtgcacggcg ccgagggccg cgcggccgag cgggaccggg
       61 cgctggcgtt gcggctgcag gacgaggtgg cccagcgggg gcgccgtgcg gtggtggtgt
      121 ccgacgcggt gtacgacgag catccggcgc tccacccgag cgcggtcatc gccattggcg
      181 ggccgggcgt caaccggttg agcgctgagc tcctgccgtc gctcccggcg gtgtgggcgc
      241 aggacgacaa gatcttcgtc caggccgcgc gcgatgcgac cccgccgcaa gcggtggtgt
      301 gggggatgga cgcggacgcg accgcggagg cggtggaggc gttcgtgttc catggggagc
      361 tcgaccggct gctgggcggc ctctggcgga gtccccgcaa gtcgctaacc tgaggatcgg
      421 acccgccggt cggcgggcgc gttcccgact ccgaggccga tgccgatgcg cttccgtgcc
      481 ctgttcgccc tggcgctgct gtcgctcgcc acctgcacca acgacctccc cgtggccccc
      541 ggcggcgccg gcgggggcga cctggccatc cggcccatct tccccgccag ctttgccgcc
      601 ttcgcgcagg ccctctcgat cgacgccgcc aaggtcgccg ccacccgccc gggcgatacc
      661 gtcgtcgtgt tccagcagtc ggtcacgttc aatccgaacg acagcgtgct cgccctgtcg
      721 gtcaaggtac cgttcacggc cgacacggcg accttcgacg tcaccctgac cttcctgtcg
      781 aaggcggggg tgccgctctt ccagagcgtc ttcccgtcgc tcacgctctc gcgctcggcg
      841 cccgtctcgc cggtcgactt ctcggtggca tacatcggcc cgggcgacag caccgtcgcc
      901 gtgtcggtct cacccaacgt cgacacggtg aacgccgaca ccacgctcca gttcagcatc
      961 tcggccattg atgtctccca ggcgccggtg acgcagctgt acgtccactg gaccagttcc
     1021 aacccggcgg cgccgatcga cgcgacgggc aagctcaagg ggccgggggt ggcggggcag
     1081 acggtggtgc acgcgctggt gccgagcctg tacggcacga tccaggattc ggcggtcatc
     1141 accttcgtcg gcgcgccgcc gccggcggcg ggatcgttcc acggctccgt ggtggacggc
     1201 gccacccagc tcgggatcgc gggcgccacg gtgcaggcgt cgctggcggg cgccgggacc
     1261 acgtacaccg ccacatcggc cgcggacggg tcgttctccc tcggtccgct ccccgccggg
     1321 gcatacgaca tctcgctcag cgccagcagc tataccggca ccaagtacct gagtgccacg
     1381 atgcccgcgg ggaccggcct gctcgtgccg ctcccgccgg tcccgctcgc cacgctcacc
     1441 gggaagcccg gcgcactgtc gggcacggtg ctcgatgcac agaccggtgc ggcgctcccg
     1501 ggggccacgg tgctcctcga ggcgtacgcc aacgcacagg ggaccacacc cgtcgcgtcg
     1561 acgacggccg acaccacggg cgccttctcg ttcacggcac agccggaagg gacgtatacc
     1621 ctcgaggtga gcgacagcgg gtacgtcacc gccacccgga cggccctggt ctacggaagc
     1681 accaccacga gcggccagaa cgtcgtgatg tccaagggcg ggctcggctt ccgggtggtg
     1741 ctcacctggg actcgctccc ccccgacctc gacctccacg gcatcgtccc cgattcgctc
     1801 ggcgcgctga gcgagatcta ctacgccaac ccgggcgata cgacgaccag ccatctcgac
     1861 tacgacgtga cctcggggta cggccccgag acgatcacga tctacaagga attccccggc
     1921 acgtacacct tcggggtcat caattactcg gccggcaccg ggctcacgcc ggacacgacc
     1981 gtgaccacgc tggcgcgaag cggcgccatc gtccgggtgt atgacaacaa cgccctcctc
     2041 gcgacgtatc ccgtgccgaa ccaggcaggg acgaggtgga aggtgttctc gtggaacggc
     2101 tcggcgctga cgacgatcaa ccagatgagc tacgataccg ccgggacggg gacctttacc
     2161 gtggcccggc ccggcgcgca cctgctgaag aagcggtagg ggagg
//
LOCUS       DASUAP010000361         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1157111, whole genome shotgun sequence.
ACCESSION   DASUAP010000361 DASUAP010000000
VERSION     DASUAP010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157111"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /gene="pth"
                     /locus_tag="VFU45_09730"
     CDS             complement(<1..497)
                     /gene="pth"
                     /locus_tag="VFU45_09730"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU5359390.1"
                     /translation="MGLGNPGPEYDGTRHNAGFWLADHLAARWQFGPFRRDGQGRVSA
                     GRRQGEDVVLIKPQTYMNRSGAALASLRSLAGFDPARDLLVLVDDVALPAGKFRLRGA
                     GSAGGHNGLKSIEGTLRRQDYPRLRIGVGPVPPDAGDLADFVLAAPSRDDHALITELL
                     EPMSQA"
     gene            complement(513..1211)
                     /locus_tag="VFU45_09735"
     CDS             complement(513..1211)
                     /locus_tag="VFU45_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEU5359391.1"
                     /translation="MSKQLTLSAQTRQGQGKGAARSLRRAGQVPAVIYGHGRAAEPLA
                     LDDAMLQKVLLTAHGSTLLDVAIDGRAPVKALLREVQRDALRPTTVLHVDLFEVRADE
                     KITVQVAVHLTGTADGVRNGGGVLDHTLRELTIKVLPADIPDRIDVDVTPLLIGHAVH
                     VRDVKVPNAEILNDAGLTICVVVPPRTEEVVAPVAEAATAAEPELIRKAKAEGEEGEG
                     TEAAAPAAGAKPKG"
     gene            complement(1228..2181)
                     /locus_tag="VFU45_09740"
     CDS             complement(1228..2181)
                     /locus_tag="VFU45_09740"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU5359392.1"
                     /translation="MADISLSRSQMLLLSGSANRALAEEVAAQLGQPLCRVTVRRFAD
                     GELFVKIDENVRGRDVYIIQPTNPPAENIIELLLLMDAAKRASAARVTAVIPYFGYAR
                     QDRKDQPRVAISAKLMANMVSLAGADRVLAIDFHQHQLQGFFDLPVDHLYAAPVFVNH
                     YRQKTLRDLVVVAPDVGSAKMARGFAKRLNGSIAIIDKRRPSANVAEVVNVVGEVAGK
                     DCIIPDDMIDTGGTMAEAVHALKRLGAEDIYICATHALLSGPAVERLMASPVKEVAVT
                     NTIALPPERQFDRLKVLSIAGLVAKAIGYTHSDQSVSSLFD"
     gene            complement(2371..2441)
                     /locus_tag="VFU45_09745"
     tRNA            complement(2371..2441)
                     /locus_tag="VFU45_09745"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2407..2409),aa:Gln,seq:ttg)
     gene            complement(2495..3355)
                     /gene="ispE"
                     /locus_tag="VFU45_09750"
     CDS             complement(2495..3355)
                     /gene="ispE"
                     /locus_tag="VFU45_09750"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEU5359393.1"
                     /translation="MTDRISLPAPAKLNLFLRVLAREADGFHSIETLFCRLDLADELT
                     AERQSGTEVSIEVAGADTGPAADNLAVRAARLVLEATGRRFGVHLTLTKRVPVRAGLG
                     GGSSDAATALTAVNRLAGDAIPRHELLQLAARLGSDVPFFASGATLALGWGHGERMLV
                     LPGLPRASGLLVIPERGVSTAEAYQWVDGARDGAGRRGAVALSAESLATWGDVARMAG
                     NDFESPVFGRRPEVRAAFEVLATTHPLVCRMSGSGSALFAIYRTAGEREDAKLRLGRK
                     HGAIIGFETA"
     gene            complement(3352..4386)
                     /locus_tag="VFU45_09755"
     CDS             complement(3352..4386)
                     /locus_tag="VFU45_09755"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU5359394.1"
                     /translation="MTVPGTPPTRFGRRGLALALGIVISGVALWWALRGVAWGDIRAR
                     LGAVHPWTLLAAVAVATTTFPIRAWRWRYLLRTDDGRTLPWPALWHPVAIGFMANNIL
                     PARLGEIVRCYTVSRLAPVKVTASLSSVAVERVFDALTLAALFAIALLGPAVPAGIGI
                     AGKVKFVGLAGVVALLVLTAAAFRPEWAERTFRRLLPKGRVADRLAALLRGLLDGLTA
                     LHDPRRLAAVVAGSALLWVVNAWGFALAFAAFGLPGDLGTALIVQTFVVFAVAAPSTP
                     GYVGVLEGAVVLALSLYGVPKDAALAAAATYHATTFIPIIVLGAWSLARTGLSLGELR
                     SASTAPAAAP"
     gene            complement(4394..>4607)
                     /locus_tag="VFU45_09760"
     CDS             complement(4394..>4607)
                     /locus_tag="VFU45_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359395.1"
                     /translation="VITAPTVAAAAAPDGVVDIKSLCYSGRGALDRALEVRRALQDVL
                     AGRGGALRPLLDELLDLIELAARPDA"
BASE COUNT          675 a         1613 c         1701 g          618 t
ORIGIN      
        1 gcctggctca tgggctcgag gagttcggtg atcagggcgt ggtcatcccg cgatggagcg
       61 gcgagcacga agtcggcgag gtcgccggcg tcgggcggga cggggccgac cccgatccgg
      121 agccgtgggt agtcctggcg ccggagcgtc ccctcgatgc tcttgagccc gttatggccc
      181 ccggccgagc cggcaccgcg gaggcggaac ttcccggcgg ggagggccac gtcgtcgacc
      241 aggaccagca ggtcgcgcgc ggggtcgaac ccggcaagcg aacggagcga tgccagtgcg
      301 gcaccgctcc ggttcatgta cgtctgcggc ttgatgagga ccacgtcctc gccctggcgg
      361 cgcccggcgc tcacccggcc ctggccgtcg cggcggaagg ggccgaactg ccagcgggcg
      421 gccaggtgat cggccagcca gaagcccgcg ttgtgccgcg tgccgtcgta ctccgggccc
      481 gggttgccga gccccacgat ggcgcgcagg gatcagccct tgggcttggc tccggcggcc
      541 ggggccgcgg cctcggtgcc ttcgccttcc tcgccctcgg ccttggcctt gcggatgagc
      601 tccggctcgg cggcagtggc cgcctcggcc accggcgcca cgacctcctc ggtgcgcggc
      661 gggacgacca cgcagatcgt gaggcccgcg tcgttgagga tctcggcgtt gggcaccttc
      721 acgtcgcgga cgtggacggc gtggccgatc aggagcggcg tcacatccac gtcgatgcgg
      781 tccgggatgt cggccggcag caccttgatg gtgagctcgc gcagcgtgtg gtcgagcacg
      841 ccgccgccgt tgcgcacgcc gtcggcggtg ccggtgaggt ggaccgccac ctggaccgtg
      901 atcttctcgt cggcccggac ctcgaagagg tcgacgtgga gcacggtggt ggggcggagc
      961 gcgtcgcgct gcacctcgcg gaggagcgcc ttgaccggcg cccgcccgtc gatggccacg
     1021 tcgaggagcg tgctgccgtg cgccgtgagg agcaccttct gcagcatcgc gtcatcgagc
     1081 gcgagcggct cggcggcccg gccgtggccg tagatcaccg ccggcacctg cccggcgcgg
     1141 cggagggagc gggcggcacc cttgccctgg ccctggcgcg tctgcgccga caacgtcagt
     1201 tgcttcgaca tgcgtctgcc cttcgtgtca gtcgaacagc gaactcacgg attggtcgct
     1261 gtgcgtgtaa ccgatcgcct tggccaccag gccggcgatc gagaggacct tcagccggtc
     1321 gaactgccgt tcgggcggca gcgcgatcgt gttggtgacg gccacttcct tgaccggcga
     1381 ggccatcagc cgctccaccg ccggccccga gagcagggcg tgggtggcgc agatgtagat
     1441 gtcctcggcc ccgaggcgct tcagcgcgtg caccgcctcg gccatcgtcc cgccggtgtc
     1501 gatcatgtcg tccgggatga tgcagtcctt cccggccacc tcgcccacca cattcaccac
     1561 ttcggccacg ttggccgacg ggcgccgctt gtcgatgatc gcgatcgacc cgttgagccg
     1621 cttggcgaac ccccgcgcca tcttggccga gcccacgtcc ggcgccacca cgacgaggtc
     1681 gcggagcgtc ttctgccggt agtggttcac gaacaccggt gccgcgtaca ggtgatccac
     1741 cggcaggtcg aagaacccct gcagctggtg ctggtggaag tcgatcgcca gcacccggtc
     1801 ggcccccgcc agcgagacca tgttggccat cagcttggcg ctgatcgcga cccggggctg
     1861 gtccttccgg tcctggcggg catacccgaa gtaggggatc accgccgtca cccgcgccgc
     1921 gctcgcccgc ttggccgcat ccatcaggag cagcagctcg atgatgttct cggccggcgg
     1981 attggtgggc tggatgatgt agacatcccg cccccgcacg ttctcgtcga tcttgacgaa
     2041 cagctcgccg tcggcaaagc gccgcaccgt gacccggcag agcggctgcc ccagctgggc
     2101 tgccacttct tcagcgaggg cccggttggc cgaaccgctg agcagcagca tctggctgcg
     2161 cgagagcgaa atatccgcca taaagagccc ggaaagtaag cggggagtac agcttaggtc
     2221 aactgaagag ggtcagtggc tctgtgggtc agtggcagag cgggtgggtg ggtggctcag
     2281 ggcgcattgg tcgggcgtca tgccccaccg tgccgccgaa tcgtcacagc ccacggaccc
     2341 actgtcccac cgccgcactg tcccacccag ttggccccgg tggactcgaa ccaccattcg
     2401 cagatccaaa gtctgctgtc ctgccattgg acgaggggcc agtcaggtaa acgtaagcca
     2461 tctatcgggt tacgggaagg cgttggccgc gtccctacgc cgtctcgaat ccgatgatcg
     2521 ctccgtgctt ccggccgagg cggagcttcg cgtcctcccg ctcgccggcg gtgcggtaga
     2581 tggcaaagag cgctgaaccc gagccgctca tccggcacac caacggatgg gtggtcgcca
     2641 gcacctcgaa ggctgcgcgg acctcggggc ggcgcccgaa caccggcgac tcgaagtcgt
     2701 tccctgccat ccgcgcgacg tcgccccagg tggccaggct ctccgcgctg agcgctacgg
     2761 cgccccgccg gccggcgcca tcgcgcgcgc cgtccaccca ctgatacgcc tcggcggtgc
     2821 tcactccgcg ctcggggatg acgagcagac cgctcgcgcg cggaagcccc gggagcacga
     2881 gcatccgctc gccgtgtccc cacccgagcg cgagcgtagc acccgaggcg aagaacggca
     2941 cgtcgctccc caggcgtgcg gcgagctgca ggagttcgtg ccgcgggatg gcgtctcccg
     3001 ccagccggtt caccgccgtg agcgcggtgg ccgcatcgct cgatccgccg cccaggccgg
     3061 cccgcaccgg cacccgcttg gtgagcgtca ggtggacccc gaagcggcgg cccgtcgctt
     3121 cgagcaccag ccgggcggcc cgcaccgcga ggttatcggc cgccgggccg gtgtcggcgc
     3181 cggcgacctc gatcgagacc tccgtgccgc tctggcgctc ggcggtgagc tcatcggcca
     3241 ggtcgagccg gcagaagagc gtctcgatcg agtggaagcc atcggcctcg cgcgcgagga
     3301 cgcggaggaa gaggttgagc ttggccgggg ccggcaggga gatgcggtcg gtcacggggc
     3361 ggccgccgga gcggtggagg cggagcggag ctcgccgagc gacagcccgg tgcgggccag
     3421 tgaccaggcg cccaggacga tgatcgggat gaacgtggtc gcgtggtagg tcgcggccgc
     3481 ggcgagggcc gcgtccttgg gcacgccgta gagcgagagc gcgagcacca cggcgccctc
     3541 gagcacgccg acgtagcccg gcgtggacgg cgccgccacg gcgaatacca cgaaggtctg
     3601 gacgatgagc gcggtgccga ggtcgcccgg gagcccgaac gccgcgaagg cgagcgcgaa
     3661 gccccacgcg ttcaccaccc agagcagggc cgatccggcc acgaccgccg ccaggcgccg
     3721 tggatcgtgc agtgcggtga gtccgtcgag caacccgcga agcaatgccg ccagccggtc
     3781 ggccacccgg cccttcggca ggaggcgccg gaaggtccgc tcggcccatt cggggcggaa
     3841 tgcggccgcg gtcagcacca ggagcgccac gacgccggcg agccccacga acttgacctt
     3901 gccggcgatc ccgatcccgg cggggacggc gggaccgagg agggcgatgg cgaagagtgc
     3961 cgcgagggtg agcgcgtcga acacccgctc caccgcgacc gatgagagcg aggcggtcac
     4021 cttgaccggc gccagccggc tcacggtgta gcagcggacg atctcgccca ggcgcgcggg
     4081 gaggatgttg ttggccatga agccgatggc caccggatgc cagagcgccg gccaggggag
     4141 ggtgcgcccg tcgtcggtgc ggagcaggta gcgccagcgc caggcgcgga tggggaaggt
     4201 ggtggtggcg acggcgacgg cggcgagcag ggtccacggg tgcaccgcgc ccaggcgggc
     4261 gcggatgtcg ccccaggcaa cgccgcggag cgcccaccag agcgcgacac cggagatgac
     4321 gatgccgagc gcgagggcca acccgcgacg accgaagcgg gtgggcggag tgccggggac
     4381 ggtcacccgc gcctcaggcg tccggccgcg cggccagctc gatcaggtcg aggagttcgt
     4441 cgaggagggg acggagcgcg ccgccgcgcc ccgccagcac gtcctgcagt gcgcggcgca
     4501 cctcgagcgc gcggtcgagt gccccgcggc cggagtagca gagcgacttg atgtccacga
     4561 cgccgtcggg cgcggcggcc gcagccaccg tcggcgcggt gatcacc
//
LOCUS       DASUAP010000362         5160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1238812, whole genome shotgun sequence.
ACCESSION   DASUAP010000362 DASUAP010000000
VERSION     DASUAP010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238812"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFU45_09765"
     CDS             <1..229
                     /locus_tag="VFU45_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359396.1"
                     /translation="LADLPYLKADYLLRAGRAYIVAGKADLASAALDTVIVKYKDTPV
                     YTEAVIRLAELTKGAQPKGSDAVARLPQTTY"
     gene            complement(512..1342)
                     /gene="dapF"
                     /locus_tag="VFU45_09770"
     CDS             complement(512..1342)
                     /gene="dapF"
                     /locus_tag="VFU45_09770"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU5359397.1"
                     /translation="MSGTVFYKMTGSGNDFVMLDGRHTALAEWPAERIRAVCDRRHGV
                     GADGFVVLAPEGPAAVRMHFFNCDGSRAPMCGNASLCSTRLSFHLGLAPADMVLVTDS
                     GRLPSRTLVGPGERAEIHLGDFAAPKRVEDVALVSGEESVWFAHSGGPPHVVVVVADV
                     EGVDVAARGRELRYHRAFAPGGANVNFVSPPTPRTGDRWPMRTYERGVEAETLACGTG
                     AVGCAAVLAGLGRLTPPVEFLSRGGYPLSVRLQQTPGGPVSAVWLAGEGRLVFRGILA
                     "
     gene            complement(1339..2058)
                     /locus_tag="VFU45_09775"
     CDS             complement(1339..2058)
                     /locus_tag="VFU45_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEU5359398.1"
                     /translation="MAERALVVIPTYNEAANLPQIVPAVLARDPRLEVLVVDDNSPDG
                     TGQLADRLAAADPRVHVLHRAAKEGLGRAYLAGFRWALARDFALIFEMDADFSHDPKY
                     LPDFLAAIGEADLVLGSRYSGGVNVVNWPMSRLLLSWSANKYVRWVAGLPLTDSTGGF
                     KCFRREVLAAIDLDDVRSNGYAFQIEMSFRAWRKGFRLKEIPIVFVDRVEGQSKMSKR
                     IVREAVRMVPWLRFAALVGRL"
     gene            complement(2051..4444)
                     /locus_tag="VFU45_09780"
     CDS             complement(2051..4444)
                     /locus_tag="VFU45_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359399.1"
                     /translation="MTDPVEQHPLDRLPRWLPAVTYALFTVLAFRAYLFAPPGSMLYG
                     SDTIAAGVMLRSFFVNAVKALGHIPQWNPYLFGGLPYVEAGGGDTLYPTSILHFVLTM
                     PQALAWKLILHVFVAGFAMYYCARTFGVSRWVAYVAGGVHMLSAQILSQVYNGQDTKL
                     YCAAIFPLALGFLVRAIDRRSFAHFVWFGLVAGLLLVGHPNLAFYAWLTFGAWGLGMI
                     WVRRREGVRPLLQRLGGGILGLGVALGIAAVILFPMYHYLRNYSPRAGAGRGFDYAAN
                     YSMHWPETVGLLVGNFAGSDTGESPTYWGPNPLKGNLEFGGALVVVLGVAAVFGLKGD
                     RRRWALGATAFVAWLYAMGADTPFFHLVYDAIPPIRNFRAPSLSMFLFFIPAVLLAAL
                     LLDRIARGGPDAEYPRKVAARALLIGAGLSLVIAVFVQSAGTAGVASIFGPFDANRAA
                     ILSNNLNTMILGAWISALACGLLWFAVRGEGRGTVPAAGLLVAFLALTALDLLRVDAP
                     FIQAVPYDNFFPTDPSMDWVKGQLGPGERVLPSDLVIRQQGRLATYGIPETFGYHGNE
                     LRWYDAVTLRNFRDQALATDPRQYNGYLLQLMTSGLGRLLSIRLAILPATNIPITGWT
                     KLTGDQNVSIYRSGAALPGGAAVGRVVVEPDSTREISALWDPSFDPASAVLVPAPIQG
                     FPSAGGKGTFQITAESSDTVAATVTSDGPTVAVLSRTWHPYWTVTVDGAPGAVVRADY
                     AFIGVPLVAAGTHRLVFRYRSPVLERSEAATASTYIFVLLVSIGAGVTALRRRSARG"
     gene            complement(4441..>5160)
                     /locus_tag="VFU45_09785"
     CDS             complement(4441..>5160)
                     /locus_tag="VFU45_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU5359400.1"
                     /translation="AASFLGATRVWILSNFGKYFGSFGIVAGMALLAQGEGMSPTIAV
                     TAAVIMQALSLATGVALSGLLAAGALRDLGPLYSWGAVVIGALAIGGSAVLHSQRALD
                     RIRAFLPASTPPIRAASVRSLLIGLAGNALAWFGYGVVMVLLARGLFASPPPRLADAT
                     SAYTVSYLTGLLALFVPAGLGLRETIFTALLAPAAGLKVAAALAIASRILLTLVELAI
                     ALPFVVLRRRRAPSSTSDPQR"
BASE COUNT          909 a         1771 c         1811 g          669 t
ORIGIN      
        1 gctcgccgac ctcccgtacc tgaaggccga ttatctgctt cgtgccggcc gggcgtatat
       61 cgttgccggg aaggccgacc tggcatcggc cgcgctcgac acggtcatcg tgaagtacaa
      121 ggacacaccg gtgtacaccg aggcggtcat ccgcctcgcc gagctgacca agggcgccca
      181 accgaagggc tccgacgcgg tggcaaggct gccgcagacg acgtactagg ccgcctgaga
      241 acgagttgga gccccggcac cgaccggggc ggaaaccccg cgagcgttcg gccgacgctg
      301 gcggggtttt gcgttgcttt gggcctggtg tagtgtcgta taatagacat tatgttaagt
      361 gttccagtgg gaatgtgtgg agaacgcagt cggtgctggc atcctacctc tccccatcaa
      421 cttacggtcc taaatgtggc cggccaatgg cgtcactggg tgaccgccgc catacaacca
      481 cgcatcaatg cacccaggac cagccgactc cctacgccag gatgccccgg aagaccaagc
      541 gcccttcccc ggccagccag accgccgaaa cggggccccc cggagtctgc tgaaggcgta
      601 ccgagagcgg atagccaccc cggctgagga attccaccgg cggggtcagg cgacccagcc
      661 cggccaggac ggcggcgcac ccgaccgcgc cggtaccgca ggccagggtc tcggcttcga
      721 ccccgcgttc atacgtccgc atcggccatc gatcgccggt ccggggggtc ggtgggctca
      781 caaagttgac gttggctccc ccgggggcga acgcacggtg gtagcgcaac tcgcggccgc
      841 gcgccgcgac atcgaccccc tcgacatcgg ccaccaccac cacgacgtgc gggggtcctc
      901 cggaatgggc gaaccagacc gattcttccc cggagacgag cgccacgtcc tcgacccgct
      961 tcggcgccgc gaaatcgccc agatggatct cggcccgctc ccccgggccg acgagcgtgc
     1021 gggacgggag acgcccggaa tcggtgacca gcaccatgtc ggccggggcc agcccgagat
     1081 ggaacgagag ccgggtgctg cagagggacg cgttgccgca catcggcgcc cgcgacccgt
     1141 cacagttgaa gaagtgcatc cggaccgccg cgggaccttc cggggccagc acgacgaacc
     1201 catccgcccc caccccgtgt cgtcggtcac agacggctcg gatccgctcg gcgggccact
     1261 ccgccagggc cgtgtgtcgc ccgtccagca tcacgaagtc attcccggaa cccgtcatct
     1321 tgtagaagac cgtcccgctc atagccgccc caccagtgct gcgaaccgca gccacggcac
     1381 catccgcacc gcctcgcgga cgatccgctt gctcatcttg ctctggccct cgacccggtc
     1441 gacgaagacg atcgggatct ccttgaggcg gaagcccttc cgccacgcgc ggaagctcat
     1501 ctcgatctgg aacgcatagc cgttcgagcg cacgtcgtcg aggtcgatgg ccgccaacac
     1561 ctcccgccgg aagcacttga agccgcccgt cgagtccgtg agcggaagcc cggccaccca
     1621 ccggacgtac ttgttggccg accaggagag gaggagccga ctcatcggcc agttcaccac
     1681 gtttacgccg ccggaatacc gggacccgag caccaggtcc gcttcgccga tcgcggcgag
     1741 gaaatcgggg aggtacttcg ggtcgtggga gaagtcggca tccatctcga agatgagcgc
     1801 gaaatcccgg gcgagcgccc agcggaaccc ggcgagatag gcccgcccca gcccctcctt
     1861 ggccgcccga tgcaggacgt gcacccgggg atcggcggcg gcgaggcggt cggcgagctg
     1921 cccggtgccg tcgggcgagt tgtcgtcgac cacgagcacc tcgagccgcg ggtcgcgggc
     1981 caggaccgcc gggacgatct gcgggaggtt ggccgcttcg ttgtacgtcg ggatcaccac
     2041 gagggcgcgc tcagccacgg gcgctccgcc gccggagggc cgtcacaccg gccccgatgg
     2101 acaccagcag gacgaagatg tacgtggacg ccgtggccgc ttcggagcgc tcgagtaccg
     2161 gggagcgata gcggaagacc aggcggtgcg tgcccgccgc cacgagcggc accccgatga
     2221 acgcgtagtc ggcccggacc acggcaccgg gggccccgtc cacggtgacg gtccagtacg
     2281 ggtgccaggt ccggctgagc accgccaccg tcgggccgtc ggacgtgacc gtggcggcga
     2341 ccgtatcgct cgattcggcg gtgatctgga aggtgccctt tcctccggcg gagggaaatc
     2401 cttggatcgg agctgggacg aggaccgcgc ttgccgggtc gaagctcggg tcccagagcg
     2461 ccgagatctc gcgggtgctg tccggctcga ccaccacacg gccgacggcc gcaccgccgg
     2521 gcagggcggc cccgctccgg tagatgctca cgttctggtc gccggtgagc ttggtccagc
     2581 ccgtgatcgg gatgttggtc gccggaagga tggccaggcg gatgctgagc aggcgtccga
     2641 gcccgctggt catcagctgc agcaggtacc cgttgtactg gcggggatcg gtcgccaacg
     2701 cctggtcgcg gaagttccgc agggtgacgg cgtcatacca ccgcaactcg ttgccgtggt
     2761 acccgaaggt ctcggggatg ccgtaggtcg cgagccggcc ctgctggcgg atcaccaggt
     2821 ccgatgggag gacccgctct cccgggccga gctgaccctt gacccagtcc atcgacgggt
     2881 cggtcgggaa gaaattgtcg taggggaccg cctggatgaa cggcgcgtcg acccgcagga
     2941 gatcgagtgc ggtgagggcc aggaacgcca ccagcagccc cgcggcgggg acggtccccc
     3001 tgccctctcc gcgcacggcg aaccagagga ggccgcacgc gagcgcggag atccaggcgc
     3061 ccaggatcat ggtattgagg ttgttggaga ggatcgcggc gcggttggca tcgaacggtc
     3121 cgaatatcga tgcgacgccc gccgtccctg cgctctggac gaacaccgcg atgaccagcg
     3181 acagcccggc accgatcagc agggcccggg cggcgacctt ccgcgggtac tccgcatccg
     3241 gaccgccgcg ggcgatccga tcgaggagga gcgcggcgag cagcaccgcc ggaatgaaga
     3301 agaggaacat cgacaggctg ggcgcccgga agttccggat cggggggatg gcgtcgtaga
     3361 ccaggtgaaa gaacggtgtg tcggccccca tcgcgtacag ccaggcgacg aacgcggtgg
     3421 cgccgagggc ccagcggcgc cggtcgccct tgaggccgaa gaccgccgcc acgccgagca
     3481 cgacgaccaa cgccccgccg aactccaggt tccccttcag cggattcggt ccccagtatg
     3541 tcggtgattc cccggtgtcg cttcccgcga aattcccgac caggagtccc acggtctccg
     3601 gccagtgcat cgagtagttg gccgcgtagt cgaagccccg gcctgcgccg gcgcgggggg
     3661 agtagttccg gaggtagtga tacatcggga agaggatcac cgcggcgatg ccgagcgcca
     3721 cgccgagccc gaggatcccg ccgcccaggc gctgcagcag cgggcggaca ccctcgcggc
     3781 gtcgcaccca gatcatgccc aggccccatg cgccgaaggt gagccaggcg tagaaggcga
     3841 gattcgggtg gcccacgagg agcaggcccg ccaccaggcc gaaccacacg aaatgggcga
     3901 acgaccgtcg atcgatggcc cggaccagga acccgagcgc cagcgggaag atcgccgcgc
     3961 agtaaagctt cgtgtcctgg ccgttgtaca cctggctcag gatctgggcg gagagcatgt
     4021 gcaccccgcc cgcgacgtac gccacccagc gggagacccc gaacgtccgc gcgcagtagt
     4081 acatcgcgaa cccggccacg aacacgtgga ggatgagctt ccacgccaag gcctgcggca
     4141 tcgtgaggac gaagtggagg atggaggtcg ggtagagcgt gtccccaccg ccggcttcca
     4201 cgtacggcag ccccccgaac agataggggt tccactgcgg gatgtggccc agcgccttca
     4261 cggcattgac gaagaacgag cgcagcatga ccccggcggc gatcgtgtcg ctgccgtaca
     4321 gcatgctgcc cggcggtgcg aacaggtagg cccggaatgc cagcaccgtg aacagcgcgt
     4381 acgtcacggc cgggagccag cgtggcaggc ggtccagcgg gtgctgctcg acgggatcgg
     4441 tcatcgttgt gggtcggagg tggatgatgg cgctcggcgg cgccgcagga ccacgaaggg
     4501 caaggcgatg gccaactcca ccagcgtcag caggatccgg ctggcgatcg cgagcgctgc
     4561 ggccaccttg agcccggcgg cgggcgccag caaggcggta aatatagtct cccgcaggcc
     4621 caggcccgcc gggacgaaga gcgccaggag gccggtcagg taggacacgg tgtaggcgct
     4681 ggtcgcgtcg gccaggcggg ggggcggcga tgcgaagagc ccgcgcgcca gcaggaccat
     4741 cacgaccccg tagccgaacc acgccagggc gttccccgcc aggccgatca ggagcgaccg
     4801 gacgctcgcg gcgcggatcg gcggcgtgct ggcaggcagg aatgcgcgga tccggtcgag
     4861 ggcgcgctgc gagtggagca ccgccgaccc gccgatggcg agcgccccga tgacgacggc
     4921 gccccagctg tacagggggc cgaggtcgcg gagcgcaccg gcggcaagga gcccgctgag
     4981 cgccacgccg gtcgcgagcg agagcgcctg catgatgacc gcggcggtca cggcgatcgt
     5041 cggcgacatc ccttcgccct gggccaggag cgccatcccc gccacgatcc cgaacgaccc
     5101 gaagtacttg ccgaagttgg acaggatcca cacgcgggtg gcgccgagga acgacgccgc
//
LOCUS       DASUAP010000363         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_489970, whole genome shotgun sequence.
ACCESSION   DASUAP010000363 DASUAP010000000
VERSION     DASUAP010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489970"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /locus_tag="VFU45_09790"
     CDS             complement(<1..1639)
                     /locus_tag="VFU45_09790"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5359401.1"
                     /translation="MRRTAFVAALAILAAPALSAQGLYMPRAVKRTYVNGTRSFDGNP
                     GPKYWQDHGRYSITISMMPPARQVSGSEQITYTNESPDTLKVLVIKLFPNSHRPGAPR
                     DGGTSEDGLTSGVRVTSFAVDGQPQLWDASPRYFTSQPVRLPKALAPGASVQLAFQWA
                     YDLPKQPSRQGVIDPTTFYVAYFYPRVAVYDDYNGWDTMDHAGHEFYSDFNDYDVTVK
                     VPANYVVWGTGTLTNAADVLQPSALQRFQASFTSDTTIHVATGAQIHAGQVTTRGAVN
                     SWHFTATDVPDMAFGVSDHYDWDAASVQVDDAAHRRASVQAAYNDTAADFHQMVKFAA
                     HSLDFLSHQWPGVPYPYEKMTAFQGGAGMEYPMMANDESYPDPAFAQFVVAHEIAHTY
                     MPFYMGINETRYAFMDEGWATTFEYLINTLDMGADTATTLFQRFRVDNWAHNPSPLQD
                     LPVITPADALTGQAYGINAYGKPSLAYLAMKDMLGDALFRQCLHAYMARWHGKHPTPW
                     DFFYTFNNVSGQNLDWFWNSWFFSNAYIDLGITGVRRG"
     gene            complement(1719..1931)
                     /locus_tag="VFU45_09795"
     CDS             complement(1719..1931)
                     /locus_tag="VFU45_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359402.1"
                     /translation="MPEHNTPLQRPIGWTGALRWLIAVVATMAVGAAFYEGRYALGAA
                     GTGFLIVAVVLAVRAARSRRDAGGNF"
     gene            complement(2023..2472)
                     /locus_tag="VFU45_09800"
     CDS             complement(2023..2472)
                     /locus_tag="VFU45_09800"
                     /inference="COORDINATES: protein motif:HMM:NF020413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEU5359403.1"
                     /translation="MAGGDVPYDLDRFVEAQAESYERALAEIRGGRKRTHWMWYVFPQ
                     HAGLGMSEMSRRYAISGLDEARAYLAHPVLGPRLVACAEAALAIEDRSAHEVFGSPDD
                     LKLRSCATLFAAAGPPGSVFERLLDRYWKGERDPATVRLLAAHPQSP"
     gene            complement(2459..3070)
                     /locus_tag="VFU45_09805"
     CDS             complement(2459..3070)
                     /locus_tag="VFU45_09805"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU5359404.1"
                     /translation="MIHPAPVTLEGFGVRLEPLAPAHEPALRGAAADGRLWELWYTAV
                     PAPELTGKYIADALSGLQAGHMLPWAVRELATDTIVGSTRYHDIVAAIDRVEIGYTWY
                     ATRWQRSHVNTACKLLLLGHAFDALGCQVVGLRTDNFNFRSQRAIAALGAHLDGVLRH
                     HQARRDGTVRDSVMFSILAAEWPDVTRHLETRLERHGGPDGGR"
     gene            complement(3067..3681)
                     /locus_tag="VFU45_09810"
     CDS             complement(3067..3681)
                     /locus_tag="VFU45_09810"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEU5359405.1"
                     /translation="MTDARPTARLEALSDGVFAIAMTLLILDVRLPSTAGITGTAELW
                     GALGHLAPSVFAFVLSFGIILITWVSHHATLALVDRSSARFIYANGFLLLTVVAVPFT
                     TGLLGDFLGTDHAGPAVVLYNAVLAVQAIGWILVDGAALKDRLATGEWAIAMLRNGRR
                     NGYVAALFYALLAVAALRAPVASALVTTVSWIFWLILGIRMQRA"
     gene            complement(3692..4147)
                     /locus_tag="VFU45_09815"
     CDS             complement(3692..4147)
                     /locus_tag="VFU45_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359406.1"
                     /translation="MKTSTKVVTVTLLLAIAAFLTEPHGPFGGFWAPTPDAPAAAGIQ
                     VPLFMILGLVEALAFGLGVSFLFFGHAVLKAQEAASASLTRGAHLAISWLLLNWWAHD
                     SLHLHNGMQLNGLLMIEYGFHVTLIIAGVTLARYFLAVTGGPAPRAAGA"
BASE COUNT          635 a         1338 c         1615 g          622 t
ORIGIN      
        1 cgccgcgccg cacacccgtg atgccgaggt cgatgtacgc gttgctgaag aaccagctgt
       61 tccagaacca gtccaggttc tggcccgaca cgttgttgaa cgtgtagaag aagtcccacg
      121 gcgtggggtg cttgccgtgc caccgcgcca tgtaggcgtg gaggcactga cggaagagcg
      181 catcgcccag catgtccttc atggcgaggt aggcgaggga cggcttgccg taggcgttga
      241 tgccgtaggc ctggccggtg agcgcgtcgg ccggggtgat gacgggcagg tcctggagcg
      301 gcgagggatt gtgggcccaa ttatcgacgc ggaaccgctg gaagagcgtg gtggcggtgt
      361 cggcgcccat gtcgagggtg ttgatgaggt actcgaacgt ggtggcccac ccctcgtcca
      421 tgaaggcgta gcgggtctcg ttgatcccca tgtagaacgg catgtaggtg tgggcgatct
      481 cgtgggccac cacgaactgg gcgaacgccg ggtcggggta cgactcgtcg ttggccatca
      541 tcgggtattc catccccgcg cccccctgga aggcggtcat cttctcgtag gggtagggca
      601 cgcccggcca ctggtgggac aggaagtcga gcgagtgcgc ggcgaacttg accatctggt
      661 ggaagtcggc cgcggtgtcg ttgtaggccg cctgcacgct ggcgcgccgg tgggcggcgt
      721 cgtccacctg gacgctcgcg gcgtcccagt cgtagtggtc gctgacgccg aaggccatgt
      781 cggggacgtc ggtggcggtg aagtgccagc tgttgacggc gccccgggtc gtcacctgcc
      841 cggcgtggat ctgcgcaccg gtggcgacgt ggatcgtggt atcggaggtg aaggaggcct
      901 ggaagcgctg cagcgccgac ggctgcagca cgtcggccgc gttggtgagc gtgccggtac
      961 cccagaccac gtagttggcg gggaccttga ccgtcacgtc atagtcgttg aagtcgctgt
     1021 agaactcgtg gccggcgtgg tccatcgtgt cccagccgtt gtagtcgtcg tacaccgcca
     1081 cgcgggggta gaagtaggcc acgtagaacg tggtggggtc gatcacccct tggcggctgg
     1141 gctgcttcgg caggtcgtac gcccactgga acgcgagctg caccgaggcg cccggcgcca
     1201 gcgccttcgg caggcgcacc ggctgggaag tgaagtagcg ggggctggcg tcccacagct
     1261 ggggctggcc gtccacggcg aacgaggtga cgcgcacccc cgaggtgagc ccgtcctccg
     1321 acgtgccgcc atcccgcggg gcgccgggac ggtgggagtt ggggaagagc ttgatgacga
     1381 gcaccttgag cgtgtcaggg ctctcgttgg tgtaggtgat ctgctcgctt ccgctcacct
     1441 gccgggcggg aggcatcatc gagatggtga tgctgtaccg gccgtggtcc tgccagtact
     1501 tcgggccggg attgccgtcg aaggaccggg tgccgttgac gtaggtccgc ttgacggcgc
     1561 ggggcatgta gagcccctgc gcgctgagcg cgggggcggc aaggatcgcg agggcggcga
     1621 cgaacgcggt gcggcgcatg gtggtgcgtc ccttcctggg tggtctcacg gagcggtgcg
     1681 gatgttggcc gtctggccgg acatacgggt ccgggccgtc agaagttgcc accggcgtcc
     1741 cggcgcgacc gggcggcccg gacggcaagc acgacggcga cgatcaggaa cccggtgccg
     1801 gcggcgccga gggcataccg gccttcgtag aacgcggcgc cgacggccat cgtggcgacc
     1861 accgcaatga gccagcggag cgcaccggtc cagccgatgg ggcgctgcag gggtgtgttg
     1921 tgctcgggca tcggtccctc cgatcgtagg tcaggccggt cggccgacgg ccgacggcgg
     1981 cgccaggcgg cgctcctccg aggtggtcaa aggagggcca ggtcagggtg actggggatg
     2041 agcggccagc agtcgtaccg tcgcggggtc gcgttcgccc ttccagtatc tgtcgagcag
     2101 gcgctcgaag accgaccccg gcgggcccgc tgcggcgaag agcgtcgcgc atgaccggag
     2161 cttgaggtcg tcggggctgc cgaacacctc atgcgccgac cggtcctcga tggccagggc
     2221 cgcctcggcg cacgccacga gccggggtcc caggaccggg tgggccaggt acgcccgcgc
     2281 ttcgtcgagg ccggagatgg catagcgccg cgacatctcg ctcatcccga gcccggcatg
     2341 ctggggaaag acgtaccaca tccagtgcgt ccgcttccgg ccgccccgga tctccgccag
     2401 ggcccgctca tagctctccg cctgcgcctc cacgaaccgg tcgaggtcgt acgggacgtc
     2461 accgcccgcc atcggggccg ccgtggcgct ccaggcgggt ctcgaggtgc cgcgtcacgt
     2521 cgggccactc ggccgcgagg atgctgaaca tcacgctgtc gcgcacggtg ccgtcgcggc
     2581 gggcctgatg gtgccgcagg acgccgtcca ggtgggcgcc cagcgccgcg atggcccgct
     2641 gggagcggaa gttgaagttg tcggtccgga gccccaccac ctggcagccg agggcgtcaa
     2701 aggcgtgacc gagcagcagc agcttgcacg ccgtgttgac gtggcttcgt tgccagcgcg
     2761 tggcgtacca ggtgtagccg atctcgaccc ggtcgatggc ggcgacgatg tcgtgatagc
     2821 gcgtgctgcc gacgatcgtg tcggtcgcga gctcgcggac ggcccagggg agcatgtgcc
     2881 ccgcctgcag tccgctgagc gcgtcggcga tgtacttgcc ggtgagctcc ggcgcgggga
     2941 cggccgtgta ccagagctcc cagagccggc cgtcggcggc ggcgccgcgg agcgccggtt
     3001 cgtgcgccgg cgccagcggt tcgagccgga ccccgaaccc ctccagggtc acgggcgccg
     3061 ggtggatcac gcccgctgca tccggatgcc gaggatcagc cagaagatcc aggacacggt
     3121 ggtcaccagg gcgctggcca ccggtgcgcg gagggccgcg accgcgagga gggcatagaa
     3181 cagggcggcg acgtagccgt tgcggcggcc gttccggagc atcgcgatgg cccactcgcc
     3241 ggtggcgagg cggtccttca gcgccgcgcc atcgacgagg atccatccga tggcctgcac
     3301 cgccaggacg gcgttgtaga gcaccaccgc cgggccggcg tggtcggtcc cgaggaaatc
     3361 gccgaggagc ccggtggtga acggcacggc gaccaccgtc aggagcagga agccgttggc
     3421 gtagatgaac cgtgccgagg accggtccac cagcgccagg gtggcgtggt ggctcaccca
     3481 ggtgatgagg atgatgccga agctcagcac gaacgcgaac accgacggcg ccaggtgccc
     3541 gagcgcgccc cagagctccg ccgtgccggt gatgccggcg gtggagggca gccggacgtc
     3601 gaggatgagg agcgtcatcg cgatggcgaa cacgccgtca gagagcgcct cgagccgtgc
     3661 cgtggggcgg gcgtcggtca tcgggcggcg ctcaggcgcc tgcggcccgc ggcgcggggc
     3721 cgccggtgac ggccaggaag taccgcgcca gcgtgacacc ggcgatgatc agcgtcacat
     3781 ggaatccgta ctcgatcatc aggagcccgt tgagctgcat cccgttgtgc aggtgcaggc
     3841 tgtcgtgcgc ccaccagttg agcaggagcc agctgatggc caggtgcgcg ccgcgcgtca
     3901 gcgacgccga cgcggcctcc tgggccttga gcaccgcgtg gccgaagaag aggaacgaca
     3961 cgccgagccc gaaggccagc gcctccacga ggccaaggat catgaagagc ggcacctgga
     4021 tcccggccgc agccggcgcg tcgggggtcg gcgcccagaa gccgccgaac gggccgtggg
     4081 gttcggtgag gaaggcggcg atggcgagca acagcgtgac ggtcacgacc ttggtggacg
     4141 tcttcatcgg tgcggcctcg cgatcatgag tgatgctcag cgccgctcgc ccgggccgtg
     4201 gccgggtggt
//
LOCUS       DASUAP010000364         5386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_516155, whole genome shotgun sequence.
ACCESSION   DASUAP010000364 DASUAP010000000
VERSION     DASUAP010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516155"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3762
                     /locus_tag="VFU45_09820"
     CDS             <1..3762
                     /locus_tag="VFU45_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359407.1"
                     /translation="EQPYGLEGIGFSAGFDSADAVMLTWQNLYRDPVTGGAVQVRTSD
                     IDSLIRLSSGSPLETVLYVAIHPDTSGGFPIYDSVLKQVRLRWTLPARTGHPRWRTMV
                     TSLSQTGTDLSSTQYLEFWVYQDPYDGPRRADSVGARLVFDLGSVNEDALAIVPESLT
                     VSPDGVDSTFRGRRYSGVGRLDTEREPTGVFNAAVDDQGILQDRPDSLVVNGQMVTDA
                     PICRRQLSSTVPLYGWGDLDVRCTNGNGTLDTEDLNGDNVLNSNGPNDNVNRYIIDLA
                     NSPDFVRNGGPGWALFRIPLRQPDAVIGTPDMHLVQHLRITIAVPPTASPEKLARFAF
                     ARMQFVGAPWIRRAATPIQGLSGSVGLATGATDVGVVSTDNQELGYTPPPGAVDQSNQ
                     IGVISGGVQVNERALRIVATDLGPGQRAEAVGRFPAGPQNLLKYQTLRLWVRGRGAGW
                     ETHQLQAVFKVGSDDKDFYAYRTSAVTTSWLPEVVIDLGKWRQLRAQIESRWLTGQPP
                     SGSAACGGDSTAYVACDGPYLVQVGNPGINPPNLAAVQEMSAAVYDSSSATFPLAELW
                     VDDIRASDPVAQVGTAAALTAHLSASDMADVDLSLLRQDGNFQQIGQDPTYQTTTTAT
                     AGSTVRFDRFLPASLGLSVPVTIGVAHSTTDPDLLQGTDLRGADLAGLRRPVSASTSW
                     GFIVRRIATSRRWWLHGLVDPLTLSAQAANGNATSLLSTSTTSSYNFGAAYNLPITAP
                     GGVLNLTGLVRLLPGFLRHTELADGLQRPRFKVSPTNVRWSSGLTRNEANYVSYLVPV
                     VRADDSLLSASQNLAQIWRNTAGATLQPFTALTLNADVASSRDLRTYPDSTPLGRLVR
                     SQRSEFLGLDAGVERDRTLTTSLAFTPAVSRWLRPRILRTSSFVLSRYLTGRDPVRAN
                     EDTTGRFILPQTLNNTQAWEVGAAVDLAQSASAVFGDSSGMAKAFRRVRPLDVSRRTV
                     LGSTFDLAAFQPTLGYEFAFGNTADFLTQQGSPALAATDGHTDALATAADLPGGFTLS
                     AAYGLTTQSRYARVLGSYQRTDTRQTDWPTGSLRWTRPMRSGPLTLVSAGINVRQTEG
                     TTFTPAFTGSGSTGRTKSASLAPDASFAFRNGLSLTLNYSRVDQLNATSGNQTQVAQR
                     TFNATLNDVFRLPESLSRLRRQVRASLTGLRSISTTCLSQAGGPCTSVSDQRRTEING
                     AFYTDLVAIAEAGLSFGYTVNESREVNQLNTSLFVSLSMNIQLSSGGYR"
     gene            3759..4664
                     /locus_tag="VFU45_09825"
     CDS             3759..4664
                     /locus_tag="VFU45_09825"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5359408.1"
                     /translation="MIRRAAVLAAFAAACASSAPPSREFDGPQAMRYVERQLAFGPRI
                     PGTTGHDAMAAWLDSLLRARADTVVVQAWTHTPAHGPPVRMTNFLARFNPSEKHRVLF
                     LAHWDTRPISDGPNSKDRNAPVPGADDGASGVAVLLGMADALRKNAPGAGVDLLFVDG
                     EDFGDFDAPGRPDVLVGARYYADHLPTGPLPAWAVLLDMVGDKDLAINPEGYSISAAP
                     DVVERFWGMARTLGHADVFPATPIGPIIDDHTELQRVGIRAIDVIDLNYDAWHTKDDT
                     ADKLSVASLQAVGDVSMGMVRTERP"
     gene            5080..>5386
                     /locus_tag="VFU45_09830"
     CDS             5080..>5386
                     /locus_tag="VFU45_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU5359409.1"
                     /translation="MSPRRAALSLLGLAAAGHLALHWRGGPARAPGEALAAPRGTRSP
                     TAQRDSVLRATRPLGRDERIDLDRASAHELERLPGIGPGLAARIVGDREAHGPFGGLE
                     "
BASE COUNT          821 a         2017 c         1794 g          754 t
ORIGIN      
        1 gagcagccct acggcctgga ggggatcggt ttctcggcgg ggttcgactc cgccgacgcg
       61 gtgatgctca cctggcagaa cctctaccgc gacccggtca ccggcggcgc cgtgcaggtg
      121 cggaccagcg acatcgacag cctgatccgc ctgagcagcg gttcgccgct ggagaccgtg
      181 ctctacgtgg ccatccaccc ggacacctcc ggcggcttcc ccatctacga ctcggtgctg
      241 aagcaggtgc gcctgcgctg gaccctcccc gcgcggaccg ggcacccccg ttggcgcacg
      301 atggtcacgt cgctcagcca gaccggcacc gacctgtcga gcacgcagta cctcgagttc
      361 tgggtctacc aggacccgta cgacggcccc cggcgggccg actcggtcgg cgcgcggctg
      421 gtgttcgacc tggggagcgt caatgaggac gcgctggcca tcgtgccgga gtcgctcacg
      481 gtgtcgccgg acggcgtcga ctccaccttc cgcggacggc gctactccgg ggtcggacgg
      541 ctcgacaccg agcgggagcc gaccggggtg ttcaacgccg ccgtcgacga ccaggggatc
      601 ctgcaggacc gccccgattc actggtggtc aacgggcaga tggtcaccga cgcgcccatc
      661 tgcaggcgcc agctctcctc gaccgtgccg ctctacggct ggggcgacct cgacgtccgc
      721 tgcaccaacg ggaacgggac gctcgacacc gaggacctga acggcgacaa cgtcctcaac
      781 tccaacgggc ccaacgacaa cgtcaaccgg tacatcatcg acctggcgaa ctcgcccgac
      841 ttcgtgcgca acggcgggcc cggctgggcg ctcttccgga tcccgctgcg ccagcccgat
      901 gccgtcatcg gcacgcccga catgcacctg gtgcagcacc tccggatcac gatcgccgtg
      961 ccgccgacgg cctcacccga gaagctggcg cggttcgcat tcgcgcggat gcagttcgtg
     1021 ggcgcgccgt ggatccggcg ggccgccacg ccgatccagg ggctgagcgg ctcggtgggg
     1081 ctcgccaccg gtgccacgga cgtcggcgtc gtctcgaccg acaaccagga gctcggctac
     1141 acgccgcccc ccggcgccgt cgaccagtcg aaccagatcg gcgtcatcag cggcggcgtg
     1201 caggtcaacg agcgcgcgct ccgcatcgtg gccaccgatc tcggcccggg ccagcgtgcg
     1261 gaggcggtgg ggcgcttccc ggcggggccg cagaacttgc tcaagtacca gacgctccgg
     1321 ctctgggtgc ggggccgggg cgccggatgg gagacccacc agctgcaggc ggtgttcaag
     1381 gtcgggagcg atgacaagga cttctacgcc taccgcacca gcgccgtcac cacgtcgtgg
     1441 ctcccggagg tggtcatcga cctcggcaag tggcgccagc tccgggccca gatcgagagc
     1501 cggtggctca ccggccagcc gccgagcggt tccgccgcct gcggcggcga ctcgaccgcc
     1561 tacgtcgcgt gcgacggacc gtacctggtg caggtcggga acccggggat caacccgccg
     1621 aacctcgcgg cggtccagga gatgtcggcg gcggtgtacg actccagctc ggccacgttc
     1681 cctttggcgg agctctgggt ggacgacatc cgggccagcg acccggtggc gcaggtcggc
     1741 acggcggccg ccctcacggc tcacctgtcg gcctcggaca tggccgacgt cgacctctcc
     1801 ctgctccgcc aggatgggaa cttccagcag atcggccagg acccgaccta ccagaccacg
     1861 accacggcca ccgccgggag cacggtccgc ttcgatcggt tcctcccggc ctcgctcggg
     1921 ctgtcggtgc cggtcacgat cggcgtcgcc cattcgacga ccgatccgga cctcctgcag
     1981 ggcaccgact tgcgcggcgc cgacctcgcg gggctgcggc ggccggtgag cgcgtcgaca
     2041 tcgtggggct tcatcgtccg gcgcatcgcg acctcccgcc ggtggtggct ccacggcctg
     2101 gtggatccgc tcacgctgag cgcccaggcg gccaacggga atgccacgag cctgctctcc
     2161 acgtcgacga ccagcagcta caacttcgga gcggcgtaca acctgcctat cacggccccg
     2221 gggggcgtgc tcaacctgac gggcctggtg cggctcctgc ccggcttcct gcggcacacc
     2281 gagctcgccg acggtctcca gcgcccgcgg ttcaaggtga gccccaccaa cgtccgctgg
     2341 tcgagcggcc tgacccgcaa cgaggccaat tacgtctcat acctggtgcc ggtcgtccgc
     2401 gccgacgact cgctgctgtc ggcctcgcag aacctggcgc agatctggcg gaacacggcc
     2461 ggggccacgc tgcaaccgtt cacggcgctg accctcaacg ccgacgtcgc gagctcccgc
     2521 gacctccgga cgtaccccga ctccaccccc ctcggccggc tggtccggtc acagcgcagc
     2581 gagttcctgg gcctcgacgc cggcgtggag cgcgatcgga cgctgaccac gtcgctggcg
     2641 ttcacgccgg ccgtgtcgcg gtggctccgg ccccggatcc tccgcaccag cagcttcgtc
     2701 ctcagccggt atctcaccgg gcgcgacccg gtgcgcgcca acgaggacac cacggggcgc
     2761 ttcatcctgc cgcagaccct caacaacacg caggcgtggg aggtcggcgc cgcggtcgac
     2821 ctggcgcagt cggcctccgc cgtcttcggc gacagcagcg gaatggccaa ggccttccgc
     2881 cgggtgcgcc cgctcgacgt ctcgcgccgc accgtgctgg ggtcgacctt cgacctggcg
     2941 gccttccagc ccacgctcgg ctacgagttc gccttcggca acaccgccga cttcctcacc
     3001 cagcagggct cgccggcgct tgccgccacc gacgggcaca ccgacgcgct agccacggcc
     3061 gccgacctcc ccggcgggtt cacgctgtcg gccgcgtacg gcctgaccac gcagtcccgc
     3121 tatgcgcggg tgctcgggag ctaccagcgc accgacaccc gccagaccga ctggcccacc
     3181 ggttcgctcc gctggacccg cccgatgcgc agcggcccgc tgacgctggt gagcgccggg
     3241 atcaacgtgc gccagaccga agggaccacg ttcacgccgg cgttcaccgg cagcgggagc
     3301 acagggcgca ccaagtcggc gagcctggcg ccggacgcct cgttcgcctt ccggaacggg
     3361 ctgtccctga ccctcaacta ctcccgcgtc gaccagctga acgccacgag cgggaaccag
     3421 acccaggtcg cccagcgcac cttcaatgcg acgttgaacg acgtgttccg cctcccggag
     3481 agcctgagcc ggctccgccg ccaggtccgc gcctcgctca cggggttacg ttccatctcc
     3541 accacctgcc tcagccaggc cggcggaccg tgcaccagcg tctcggacca gcgccgcacg
     3601 gagatcaacg gggcgttcta caccgacctc gtcgccatcg ccgaggccgg gctctcgttc
     3661 ggctacaccg tgaacgagtc gcgcgaggtc aaccagctca ataccagcct cttcgtgagc
     3721 ctcagcatga acatccagct cagttcgggg ggataccgat gatccgtcgc gccgcggtcc
     3781 tggccgcgtt cgccgccgcc tgcgcctcgt cggcgccccc gtcccgggag ttcgacggac
     3841 cccaggcgat gcgctacgtc gagcgccagc tcgccttcgg gccccgcatc ccgggtacca
     3901 ccggccacga cgcgatggcc gcctggctcg actccctcct ccgcgcccgg gccgacacgg
     3961 tggtggtgca ggcgtggacc cacacgcccg cgcacggccc gccggtccgg atgaccaact
     4021 tcctggcgcg cttcaatccg tccgagaagc accgggtgct cttcctggcc cactgggata
     4081 cccggccgat ctccgacggg cccaactcca aggaccggaa tgcgccggtc cccggcgccg
     4141 acgatggcgc gtccggcgtg gcggtgcttc tcggcatggc cgacgcgctc cggaagaacg
     4201 cccccggcgc cggcgtcgac ctgctcttcg tcgacggcga ggacttcggc gacttcgacg
     4261 ccccgggccg ccccgacgtc ctggtcggcg cgcggtacta cgccgaccac ctgccgaccg
     4321 gcccgctccc ggcgtgggcc gtcctcctcg acatggtggg cgacaaggac ctggccatca
     4381 acccggaggg ctactcgatc tcggccgccc cggacgtggt cgagcggttc tgggggatgg
     4441 cccgcaccct gggccacgcc gatgtcttcc cggccacccc catcgggccg atcatcgacg
     4501 accacacgga gctccagcgc gtcggcatcc gcgccatcga cgtcatcgac ctcaactacg
     4561 atgcgtggca caccaaggac gacaccgccg ataagctgag cgtcgcgagc ctccaggccg
     4621 tgggcgatgt ctcgatgggg atggtacgga ccgagaggcc gtgagcgaag tgagaagtga
     4681 gaagtgaaga gtgagaagtg aaaagcgaga agtgaaaagt gaagagtgaa aagtgaagtg
     4741 tgagacggaa agaggaagag ggaagacgac aaggaaaagg agaaggagaa ggaagaggag
     4801 aagggaagag gaagagcgac cgaagaaccg agatgtcgtg agatgtccgg gacggcgggg
     4861 tgtctcgtcg agatccggtc ggcagagaaa tgatcggtgc cgcaccaaaa tgatgacggg
     4921 gggatctcag cgggtgcgag attccctctt gtcacgtatc cggggctgat cgtccgcggg
     4981 tcgcgggtcg cgggtcgcag gtcacgagtc gcgcgtctca cctctccctt ttcccttttc
     5041 acttctcact tcttacttcc tccttctccc ctccccgcca tgtccccccg ccgcgccgcg
     5101 ctgtccctcc tgggcctggc cgccgccggc cacctggcgc tgcactggcg gggcggcccc
     5161 gcccgggcgc cgggtgaggc gctggccgct ccgcgcggca cccgctcacc caccgcccag
     5221 cgtgacagcg tgctgcgcgc cacccgcccg ctcggccggg acgagcggat cgacctcgac
     5281 cgggcgagcg cccacgagct cgagcgcctg ccggggatcg gtcccgggct cgcggcgcgg
     5341 atcgtgggcg accgggaggc gcacggaccg ttcggcgggc tcgagg
//
LOCUS       DASUAP010000365         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_122117, whole genome shotgun sequence.
ACCESSION   DASUAP010000365 DASUAP010000000
VERSION     DASUAP010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122117"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFU45_09835"
     CDS             complement(<1..496)
                     /locus_tag="VFU45_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5359410.1"
                     /translation="MRPRWPVLTLVAVASFLCGGWLLQRGAAADAGGYRQARLFDDIV
                     GRVEQAYVDPVPDSVLYQKAADGLLEELHDPYTVLLRGQDYRDLQEETSGNYAGLGIR
                     IDVRNGWITVVAPLPDSPAERAGIETGDQLVSVDGRSAESWQSDAAVRRLRGPPGSKV
                     KIGIR"
     gene            complement(542..1156)
                     /gene="tmk"
                     /locus_tag="VFU45_09840"
     CDS             complement(542..1156)
                     /gene="tmk"
                     /locus_tag="VFU45_09840"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU5359411.1"
                     /translation="MSGFFLVVEGPEGAGKSTLVRALEARLRAAGHRVQMVREPGGTP
                     LAEAARALALDPASHTGPAAELFLMLAARADLVEKVIRPKLADGEVVVTDRFDLSTTA
                     YQVAGRGLPGAPVSAANSAATGGLVPDLTLVLDIPAGVGEARQARAGKALDRLERESD
                     GFHQRVHAAYRAASGPGVVHLDASRSAAEVADAAWAEIVGRLER"
     gene            complement(1153..1506)
                     /locus_tag="VFU45_09845"
     CDS             complement(1153..1506)
                     /locus_tag="VFU45_09845"
                     /inference="COORDINATES: protein motif:HMM:NF017902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEU5359412.1"
                     /translation="MIWDKAEELGRLIGQANETKALRRAEADLRGDDDTRTRLEKIQT
                     LARDVDAQVAEGKMPDPATVEAYETAVRDLETSGIGQSYVVARSNFEKLMVKVNERIS
                     EGIEKGASSNIITLG"
     gene            complement(1507..>2349)
                     /gene="prmC"
                     /locus_tag="VFU45_09850"
     CDS             complement(1507..>2349)
                     /gene="prmC"
                     /locus_tag="VFU45_09850"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU5359413.1"
                     /translation="VAVTLEALLPPARARLAQAGVEDAADEALRLWSGLWRTTPAEAY
                     LGRREAISGEVAARFERAVERRAAGEPLAYIVGWTGFRRLVVETDRRALIPRPETEGV
                     VDLLLARRRTGVVADIGTGTGCLALALADEGEFTEVHAVDASPEALALAARNAEAVGA
                     GIRFHRGDLVAPLAGVACDALVSNPPYLTAAEYAALDPSVRAWEPAEALASGADGLVH
                     TRRLLDEGRGVVRSGGWIVIELDCQRAAASAAIARHFGWAEVEVHPDLFGRERYLTAR
                     RSEA"
BASE COUNT          305 a          950 c          778 g          316 t
ORIGIN      
        1 gccggatgcc gatcttgacc ttgctccccg gcgggccccg gagccgccgc acggccgcgt
       61 ccgactgcca cgactccgcc gagcggccgt cgacgctcac cagctggtcg ccggtctcga
      121 tccccgcacg ctccgccggc gagtcgggga gcggcgccac caccgtgatc cagccgttgc
      181 ggacgtcgat ccggataccg aggccggcgt agttgccgga ggtctcttcc tgcagatccc
      241 ggtagtcctg gccccggagg agcacggtat acgggtcgtg cagttcctcc aggaggccgt
      301 cggcggcctt ctggtagagc acgctgtccg ggacggggtc gacgtacgcc tgttccaccc
      361 gccccacgat gtcgtcgaag agccgtgcct ggcggtaccc acccgcgtcg gcggcggcgc
      421 cgcgctgcag gagccagccg ccgcagagga aggacgccac cgcgacgagg gtcaggacgg
      481 gccagcgtgg tctcatacga cctccttcga acagcgacga cgcggcgtcg ctggattcaa
      541 cctagcgttc gagccgaccg acgatctcag cccacgcggc atcggccacc tcggccgccg
      601 accgcgatgc gtcgaggtgg accaccccgg ggccggaggc cgcacggtag gctgcatgga
      661 cccgctggtg gaagccgtcg gactcccgct ccagccggtc gagagccttc cccgcgcgcg
      721 cctggcgggc ttcgccgacc ccggcgggga tgtcgagcac cagggtcagg tccggcacca
      781 gcccgcccgt cgccgcggag ttcgccgcgg acaccggagc gcccggcaga ccgcgcccgg
      841 ccacctggta ggccgtcgtg gagaggtcga agcggtcggt gaccacgacc tccccgtcgg
      901 caagcttcgg gcggatcacc ttctccacca ggtccgcacg cgccgcgagc atgaggaaga
      961 gctcggccgc cgggccggtg tgcgaggccg ggtcgagcgc cagcgcgcgc gccgcttcgg
     1021 ccagcggggt cccgcccggc tcccggacca tctgcacccg gtggcccgcg gcacgcagcc
     1081 gcgcctcgag cgcgcgcacc agcgtgctct tcccggcgcc ctcgggcccc tcgaccacca
     1141 ggaagaagcc gctcatccca gcgtgatgat attgctggag gcgcccttct cgatcccttc
     1201 gctgatgcgc tcgttcacct tgaccatcag cttctcgaag ttggaccgcg ccaccacgta
     1261 cgactggccg atcccgctgg tctcgaggtc gcgcaccgcg gtctcgtacg cctcgacggt
     1321 cgccggatcg ggcatcttcc cctccgccac ctgcgcgtcc acgtcccgcg cgagcgtctg
     1381 gatcttctcc agccgcgtgc gcgtatcgtc gtcgccccgg aggtcggcct cggcccgccg
     1441 gagcgccttg gtctcgttgg cctgcccgat gagccggccg agctcttccg ccttgtccca
     1501 gatcacttac gcctcgctcc gccgcgcggt gaggtaccgc tcgcggccga ataggtccgg
     1561 atgcacctcc acctccgccc agccgaagtg ccgggcgatg gccgcgctcg ctgccgcgcg
     1621 ctgacagtcc agctcgatca cgatccaccc gccactgcgc accacccccc gcccctcgtc
     1681 caggagccgg cgcgtgtgca cgagcccgtc cgccccgctc gccagcgcct cggccggctc
     1741 ccacgcccgg accgacggat cgagggcggc gtattctgcc gccgtcagat acggcgggtt
     1801 cgacaccagg gcgtcgcagg ccaccccggc gagcggcgcc acgaggtcgc cccggtggaa
     1861 ccggatgccc gcgccgacgg cctccgcgtt ccgggccgcg agcgccagcg cttcgggtga
     1921 cgcgtcgacg gcatgcactt ccgtgaactc cccttcatcg gcgagcgcca gcgccaggca
     1981 tcccgtgccg gtcccgatgt ccgccacgac cccggtccgc cgccgggcga gcagcaggtc
     2041 caccacccct tccgtctccg gccgcgggat cagggcccgc cggtcggtct cgaccaccag
     2101 ccggcggaac cccgtccatc caacgatgta ggcgagcggc tcgccggcgg cccgccgctc
     2161 cactgcccgc tcaaagcgcg ccgccacctc gccggagatc gcctccctcc ggccaagata
     2221 ggcctccgcc ggcgtggtgc gccacagccc gctccagagc cggagggcct cgtcggccgc
     2281 atcctccacg ccagcctgcg ccagccgcgc tcgcgccggg gggagcagcg cctcgagggt
     2341 cacggccac
//
LOCUS       DASUAP010000366         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_693629, whole genome shotgun sequence.
ACCESSION   DASUAP010000366 DASUAP010000000
VERSION     DASUAP010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693629"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VFU45_09855"
     CDS             complement(<1..1119)
                     /locus_tag="VFU45_09855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5359414.1"
                     /translation="MPTEIGKYSIPAAQVGSVLGDAEIAALTDLIRSGATLSQGPLRE
                     KFEQRLRAHIGTRHALTVTSGTVALAIAVHLLDLRPGDEVIVTPQSFKATAEPLLAHE
                     KVAVRFCDVDPDTLNIDSAAFEALITPRTKALLLVHYGGRPAEMAEIMAIARRHGVLV
                     VEDCAHALGSEYRGRRPGSLGDIGCFSFHSTKNISSLGEGGMITFDRDDWAERVDRIR
                     SNAVDGDYVSEPLHVGHGRRPWMMWVDDSYENICSTVRAPGSNATMSEAGAAVGLVQL
                     DRLEALVSRRQEIAARYAEVLGAVPDDVRLPEVPPGVNHPYHLFTFFVRPESEVGRDA
                     VVAALEDAGIQVQLRYFPLHLRPEWRGRGHRYGDCPVAE"
     gene            1565..1834
                     /locus_tag="VFU45_09860"
     CDS             1565..1834
                     /locus_tag="VFU45_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5359415.1"
                     /translation="MAVLSELTDILAGLGVEPELLTPDTRLRSDLGLTSTETTELGLE
                     MTRRFGARIDLWDAADYSLAELADAISAERGAAGAERGAAGGERG"
     gene            1831..>2087
                     /locus_tag="VFU45_09865"
     CDS             1831..>2087
                     /locus_tag="VFU45_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359416.1"
                     /translation="MSAAGPSAPASPAHGAEPAPGPAAGLPTPAALPRVPAERAREYR
                     RRGWWLPERVDWLALRAAAERPEATAVVAPGGRLTYRQLADA"
BASE COUNT          303 a          709 c          755 g          320 t
ORIGIN      
        1 ttcggccacc gggcagtcgc cgtagcggtg gccgcggccg cgccactcgg gccgcaggtg
       61 gagcgggaag taccgcagct gcacctggat gccggcgtcc tctagtgccg cgaccaccgc
      121 gtcgcggccc acctcgctct cgggccggac gaagaacgtg aacaggtggt aggggtggtt
      181 cacccccggc ggcacctcgg gcagccggac atcgtcgggc accgcgccga gcacctcggc
      241 gtaccgtgcg gcgatctcct ggcggcggct caccagcgcc tccagccggt cgagctgcac
      301 gagacccacg gccgcgcctg cctcgctcat ggtggcgttg ctgccgggag cgcggaccgt
      361 gctgcagatg ttctcgtagg agtcgtcgac ccacatcatc cacggccggc gcccgtgacc
      421 gacgtgcagc ggctcgctga cgtaatcccc gtccaccgcg ttggaccgga tgcggtccac
      481 ccgctcggcc cagtcgtcgc ggtcgaacgt gatcattccg ccctcgccca gcgagctgat
      541 gttcttggtg gagtggaagc tgaaacagcc gatgtcgccg agggagccgg gacggcggcc
      601 gcggtactcc gagccgaggg cgtgcgcgca gtcctcgacc accaggacgc cgtgccggcg
      661 ggcgatcgcc atgatctccg ccatctcggc gggccggccg ccgtagtgga ccagcagcag
      721 cgccttggtg cgcggggtga tcagcgcctc gaacgcggca ctgtcgatat tgagcgtgtc
      781 ggggtcgacg tcgcagaacc ggaccgccac cttctcgtgg gcgagcagcg gttccgcggt
      841 ggccttgaac gactgcgggg tgacgatcac ctcgtccccg ggccgcaggt cgagcaggtg
      901 caccgcgatg gcgagggcga ccgtcccgct ggtgacggtg agcgcgtgcc gggtgccgat
      961 gtgggcgcgc aagcgctgtt cgaacttttc tctgagcggt ccctgggaga gcgtggcacc
     1021 ggaacggatc aagtcggtga gggccgcgat ttcggcgtcg ccgaggacac tccccacctg
     1081 ggcagcaggt attgaatact taccgatctc cgttggcatg tgggcggtcc tagctcgctg
     1141 aaggagtgga tatgaccgcc ggggagctta gattcacggc ggtgagaacg caaagggtgg
     1201 gcttccggcc aacggggcgt gttctggtca agggaccggc aactcgaaac ggagcggggt
     1261 acttacacat accgtcctga ccagggattt cattgattca tgcggctctg gagggagata
     1321 ttccgctcgt tggggagagg ttgacgaagt cgccgacaag gtgatgatgg ggtctggcga
     1381 gattccggat cgggcccgcg gcggccagtt cgggccgccg tgcccgtgga cgtcattgac
     1441 agttccgaga cggcggggta cttgctgggc cgtaattccg gtgatccgtt ttccaccccg
     1501 gtgtcggggc atacctgacc ccatttcttg atcgccttcg ccctcccgga aggagagcgt
     1561 agccatggct gtactcagtg aattgacgga tattctcgcg gggctcgggg tcgagcccga
     1621 gctgctcacc cccgacaccc ggctccgctc cgacttggga ctcacgtcca cggagaccac
     1681 cgagctcggc ctggagatga cacgccgctt cggcgcccgg atcgacctgt gggacgccgc
     1741 cgactacagc ctggccgagc tcgccgacgc catcagcgcg gagcgcggcg cggcaggtgc
     1801 ggagcgcggc gcggccggcg gggagcgcgg atgagcgccg ccgggccctc ggcccctgcc
     1861 tcccccgccc acggagccga gcccgccccc ggtcccgccg cggggctccc aacgcccgcc
     1921 gccctacccc gggtaccggc ggaacgcgcc cgggaatacc ggaggcgcgg ctggtggctg
     1981 cccgagcggg tggactggct ggcgctgcgc gcggccgcgg agcggccgga ggccaccgcc
     2041 gtcgtggccc cgggcgggcg gctgacctac cgccaactcg ccgacgc
//
LOCUS       DASUAP010000367        10054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_490124, whole genome shotgun sequence.
ACCESSION   DASUAP010000367 DASUAP010000000
VERSION     DASUAP010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10054
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490124"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="VFU45_09870"
     CDS             <1..1484
                     /locus_tag="VFU45_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5359417.1"
                     /translation="AERFLLPVLDILQSIPVLGFLPGLVLGLVSLFPDRNAGLELAAI
                     LMIFTAQAWNLALSFYNSLKSVPEPLREAAQVAGWGPWTTFRRLEVPSAAPALVWNGM
                     LSMAAGWFFLMVNEAFQLGGRDFRLPGIGAYMSAAIDAGDRRAMVLALAAMVVMIVAV
                     DRLFWRPLVVWAQKFRLDDSSGEAPPTNWLLELVRRSRLRVQARSVRHTAVATARVAT
                     LPIRHSLRQADRVLRRFPGPRPGRDGVVRVSATVLGVLLVAGAGFGVLQLVRLLVRLD
                     AAAWLLVARSAAYTFLRVLAAVALSTAWALPVGVLIGRSPRISRVFQPVIQIVASFPA
                     PMLFPLVILGLLRFGAGMGIGAVTLMVLSAQWYILFNVISAVAALPPQLADAAEVFRM
                     TGWPRWRRLYLPAAFPALVTGWVTAAGGAWNGSIVAEYVEAGGTTHIANGLGSLISLA
                     TVHADFPLLAGGIAAMSVIVVGWNRLVWRPLATLAQTQFGVTQ"
     gene            1481..2812
                     /locus_tag="VFU45_09875"
     CDS             1481..2812
                     /locus_tag="VFU45_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU5359418.1"
                     /translation="MTDATRGSEVLLEARGVIQRFRLPKQAEFEALRDISLTIREREV
                     VALVGPSGCGKSTLLRLLAGLTRPTAGQVLYRGQQVEGVLASAAMVFQSFALLPWLTV
                     AENVAMGLAARGRQGTDHEAAVKHAIEAVGLDGFEQAYPRELSGGMKQRVGFARALAV
                     EPEILFMDEPFGALDALTAENLRSQVVDLWQNPDTGVSTLVVVTHSVDEAVYLAGRIV
                     VFGTNPGHIREVLDNPLPYPRDPRSPQFSAMVDRLHAILTATLLPEPERAAGGVAARL
                     VPFPRVHVSEVMGLLDHLADRAEGGDSVFDIAEQIGVDYDRMTSVVRASEQLGWVVTP
                     GEDVRLTTAGQQLVAGDSAERKALTRVRVLEHPLFARVVGMIKENDGAIEVDEVLADL
                     TICFPFSRANGLLRTVIEWGRFAEVLDHESRNRRLVLPEEERAALEASADE"
     gene            complement(2976..3431)
                     /locus_tag="VFU45_09880"
     CDS             complement(2976..3431)
                     /locus_tag="VFU45_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5359419.1"
                     /translation="MPVLDGEKVVAALRGAFAREAGRTALLQLAADRIRAAGSPYTSV
                     YLYMLQDGELVLEAHSGRETEHTRIAVGHGVCGTAVATGLDQNVGDVRARENYIACNL
                     FTRSELVVLIRLGNDILGQIDIDSDALDPFTPEEEAEVKKVADALGVLL"
     gene            complement(3431..4702)
                     /locus_tag="VFU45_09885"
     CDS             complement(3431..4702)
                     /locus_tag="VFU45_09885"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5359420.1"
                     /translation="MRILTTAALLLAFGASTPLVAQQGGEAQDLLNDVKFLASDALQG
                     RKIGTPGADTAAAYLARRFQAAGLQPAPGGWMQRFTVDPTAPAAQHSGIGGAHGENVI
                     GVLPGRDPVLEDQVIVVGAHYDHLGLGGFGSLAPDSTGKVHNGADDNASGSAALVAIA
                     RRLAAHRPARTVVFIAFSGEEEGLLGSEWYVKHPLYPLDHTVAMINLDMVGRLRDSKL
                     IVYGSATAQEWPALLDSLNATAHFELKEHGDGYGPSDQSSFYAAHRPVLHLFTDLHGD
                     YHRPTDDWQKINADGLARVADFAAAIATAVGDRRTGLTFVDVPQPAVAADSTPAVTPG
                     YGAYLGTIPDMSEAPGGVALTGVRAGSPAEKAGIRAGDVIVRMDGKDVKDLMGMTEVL
                     RAHKAGDTIEIVVRRNGVDVVTHATLGVRGD"
     gene            4741..5340
                     /locus_tag="VFU45_09890"
     CDS             4741..5340
                     /locus_tag="VFU45_09890"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5359421.1"
                     /translation="MTDRIHPMNTRHLALIPTCIALNVAMGKVVQELGLPIYLDAVGT
                     ILVTALLGPAAGVLTGALSQALTALLSGQMMWLAFLPIQVLIAIVAGVAAQRGGFRTV
                     GWAVGWGALCGLLGGVCSAVISYLLFKGVTAPGVTALGALFRSLGFSLERAVTAASIS
                     TDLVDKGIAFGLVAAVLLALPARMASRFPSAVRATGRGA"
     gene            5337..6095
                     /locus_tag="VFU45_09895"
     CDS             5337..6095
                     /locus_tag="VFU45_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU5359422.1"
                     /translation="MTAGSPASPIGRAHPFTPLTATLAVVFLAFLLPAPAGPVVLALL
                     AVACAPLVGDAAALYPALIIVVPLWLLLFLVLGVLGDAPFTALGPVTLSVAGTREALA
                     QGARLTAIVVPSLLALRGFRAARFVDAAAERGWPFSAAYLVAATLQTIPRLRHRVILI
                     RDAQRSRGLKVRGSIVQRVRALVPLTLPLILGALADADDRAVALESRGVAAVVVPGAR
                     RTPLDPPADSILDRTIRWGAVLAVVLAAAWRVVR"
     gene            6077..6937
                     /locus_tag="VFU45_09900"
     CDS             6077..6937
                     /locus_tag="VFU45_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5359423.1"
                     /translation="MAGRPVTVRFDGVSFTYPGSAVPALRDLSLTLAGGQVTWLFGRL
                     GAGSSTALLVAGGFAPILTGGERRGAVEVAGLDPGDPATRAALAGRVAYVSAQPHLQL
                     SGLAETVFEEVAFAPANLGWSRARIADLVPQALARIGAAHLAGRVPSELSGGELQRVV
                     IAAMLVLAPEVWLLDEPASALDPGARAAAYALFREEARRGAAVVIATEDADGALEAAD
                     RIVVLENGAVAQDGVPAAVLASDACWLLGAGSTTVGELWRAAMERAPAMARGQVPLTL
                     AAAAARVAGR"
     gene            6934..7737
                     /locus_tag="VFU45_09905"
     CDS             6934..7737
                     /locus_tag="VFU45_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5359424.1"
                     /translation="MNIASARDLRFRYGDRETIAGVSFDVAPGEGVALLGPNGAGKTT
                     LLRLLMAFLQPRSGSLDVAGRSTRGLFPEDLAGLVGFLFQRPEDQLFRRTVRDDARFG
                     PETLGWDPAHADAAVDAALRELGLDDAAHVHPYDLPLPRRRLVALAGVLALEPKLLLL
                     DEPTATLDRTARRMVVGALRARRERGIAVIAVTHDIGFAAELCDRALALDAGRIVADG
                     PIADVLGRPGAAPLPVGAELVRRVGATARSLRWDDLVSALASRCRDLQE"
     gene            7771..9333
                     /locus_tag="VFU45_09910"
     CDS             7771..9333
                     /locus_tag="VFU45_09910"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5359425.1"
                     /translation="MSAPLSVVSDLWNVVALLRDRPNAKEEHKTAFRTLVSTLGVSPL
                     SLTATPEGLAYQGVLVPEETVGAAALTRQMLQHGVGEIELPEGTGAAPLLSLLRALAA
                     PNGSYRHIQEFITAVGEPGASRFRMAAFVAPPPAAPVVSARASRAAAAPPAPRVTTGS
                     PPPRGSAELALGPGVMSSEEGGMLHFMSSQHEAIARLDELSLAIEAAPSGVNVGHLLS
                     ELVGAADAAAQKSDWEGILRAAVRVIGAESKVTSKDEGRLYSISLKRLLPRRTLDEIA
                     RFLPKPALKHDAMTVMQRMGESATDVLLDFLAAAPTIEERRHYFDALVTSAQGSDMVV
                     RTLEHGEWFVIRNVAELCGELKLESAVPVLARHLTHPDERVKRAAIVALAKIGTPATV
                     EPLRNALKDAAPAVRMQAVMVIDGKQNRALGLRLVSILEEETVPEVLREVCYSVGRSG
                     SNEGVQALIRMASPGGKLFNKKPTQQRVWAIEGLRMAGGSSAQGALEGLFQDSDKEVR
                     EAAQKALSGMGR"
     gene            complement(9514..>10054)
                     /locus_tag="VFU45_09915"
     CDS             complement(9514..>10054)
                     /locus_tag="VFU45_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5359426.1"
                     /translation="GEGRKGKKGKGTAGSGKRGALEANVTAVATTQEEIGYTGGGART
                     SATTLDATVAIAVDVTHATDYPGIEKRKHGDFRLGGGPVISRGSAVNPVVFSLLVEAA
                     EREKIPYTVEAAPRATHTDADNIFTAHRGVATGLVSVPLRYMHSPNEMVALEDLDRAA
                     RLLAAFARRVGPDTSFVPV"
BASE COUNT         1225 a         3577 c         3702 g         1550 t
ORIGIN      
        1 gggcggagcg cttcctcctc ccggtgctcg acatcctgca gagcatcccc gtcctcggct
       61 tcctgccggg gctggtgctc gggctcgtgt cgctctttcc ggaccggaac gcggggctcg
      121 agctcgcggc gatcctgatg atcttcaccg cgcaggcgtg gaacctggcg ctcagcttct
      181 acaactcgct caagagcgtg cccgagccgc tgcgcgaggc ggcccaggtg gcgggatggg
      241 ggccgtggac caccttccgc cggctcgagg tcccgtccgc cgcgcccgcc ctggtgtgga
      301 atgggatgct gtccatggcg gcgggctggt tcttcctgat ggtcaacgag gccttccagc
      361 tcggcgggcg cgacttccgc ctccccggga tcggggccta catgagcgcg gcgatcgacg
      421 ccggcgaccg ccgcgccatg gtgctcgcgc tcgcagcgat ggtcgtgatg atcgtcgcgg
      481 tggaccggct cttctggcgg ccgctcgtgg tgtgggccca gaagttccgg ctcgacgact
      541 cgagcggcga ggcgcccccc accaactggc tgctggagct ggtccgccgg tcgcggctcc
      601 gggtgcaggc gcgctcggtg cggcataccg cggtcgcgac ggcgcgcgtc gcgacgctgc
      661 cgatccggca ttcgctccgc caggccgacc gggtgctgcg ccgcttcccg ggaccgcgcc
      721 cggggcgcga cggcgtggtc cgcgtgagcg ccaccgtgct cggagtgctc ctcgtggccg
      781 gcgccgggtt cggcgtgctg cagctcgtcc ggctcctcgt ccgcctggat gcggcggcgt
      841 ggctcctggt ggcccggtcg gcggcctaca ccttcctgcg ggtgctcgca gcggtggcgc
      901 tctcgaccgc atgggccctc ccggtgggcg tgctcatcgg ccggtcgccc cggatctcga
      961 gggtgttcca gccggtgatc cagatcgtgg cgtcgttccc tgcgccgatg ctctttccac
     1021 tcgtcatcct cgggctcctc cgcttcgggg ccgggatggg gatcggcgcc gtgacgctga
     1081 tggtgctctc cgcgcagtgg tacatcctgt tcaacgtgat ctcggccgtc gccgcgctcc
     1141 cgccgcagct cgcggacgcg gccgaggtgt tccggatgac cgggtggccg cgctggcgcc
     1201 ggctctacct gccggccgcg ttccccgcgc tcgtgaccgg gtgggtcacc gcggcgggcg
     1261 gggcatggaa cgggtcgatc gtggcggagt acgtcgaggc ggggggcacg acgcacatcg
     1321 ccaacgggct cgggagcctc atctccctgg ccaccgtgca cgccgacttc cccctgctgg
     1381 cggggggcat cgcggcgatg agcgtcatcg tggtgggatg gaaccggctg gtgtggcgcc
     1441 cgctcgccac gctggcgcag acccagttcg gggtgaccca atgaccgacg cgacccgggg
     1501 aagcgaggtc ctgctcgagg cgcggggcgt catccagcgc ttccggctcc ccaagcaggc
     1561 cgagttcgag gcgctccgcg acatttcact cacgatcagg gaacgggagg tcgtggcgct
     1621 cgtcggcccc tcggggtgcg gcaagagcac gctcctccgg ctcctcgccg gcctcacccg
     1681 gccgaccgcg gggcaggtgc tctaccgcgg acagcaggtg gagggggtgc tcgcctcggc
     1741 ggcgatggtc ttccagtcgt tcgcgctcct gccctggctc accgtggcgg agaacgtcgc
     1801 gatgggcctc gccgcccggg gccgccaggg caccgaccac gaggccgccg tcaagcacgc
     1861 gatcgaggcg gtcgggctcg atgggttcga gcaggcgtac ccgcgcgagc tctcgggggg
     1921 tatgaagcag cgggtcggtt tcgcccgcgc gctcgcggtg gagcccgaga tcctcttcat
     1981 ggacgagccc ttcggcgcgc tcgacgcgct gaccgcggag aacctccgct cgcaggtggt
     2041 ggacctctgg cagaaccccg acaccggcgt cagcaccctc gtggtggtga cgcacagcgt
     2101 ggacgaggcg gtgtacctgg ccgggcggat cgtcgtgttc ggcaccaacc cgggccacat
     2161 ccgtgaagtg ctcgacaacc cgctccccta cccgcgggac ccgcggagcc cgcagttctc
     2221 cgcgatggtg gaccgcctcc acgccatcct cacggcgacc ctcctccccg agccggagcg
     2281 cgcggcgggc ggggtggcgg cgcggctggt gccgttcccg cgggtgcacg tctcggaggt
     2341 gatgggcctc ctcgaccatc tggcggaccg cgccgaggga ggggactcgg tgttcgacat
     2401 cgccgagcag atcggcgtcg actacgaccg gatgacgtcg gtggtgcggg cgtcggagca
     2461 gctgggctgg gtggtgaccc cgggcgagga cgtgcggctc accaccgcgg gccagcagct
     2521 cgtcgccggc gacagcgccg agcggaaggc gctcacccgg gtgcgggtgc tcgagcatcc
     2581 gctcttcgcg cgcgtggtgg ggatgatcaa ggagaacgac ggcgcgatcg aggtggacga
     2641 ggtcctggcc gacctgacca tctgcttccc gttcagccgc gccaacgggc tcctgcggac
     2701 ggtcatcgag tgggggcgct tcgccgaggt gctggaccac gagagccgca accggcgcct
     2761 ggtgctgccg gaggaggagc gggcggcgct ggaggcgagc gcggacgagt agggggtgcg
     2821 atccgctggt cgctatccgt catccgctat ccgttatccg ctatccgtta tccgctatcc
     2881 gatgctccgc gaacgaacgg gcctccggcc aagtgaccgg aggctcgatc tggttcggcg
     2941 catccagtat cgggcgacgg atagcggata gcgccctaca acaacactcc cagcgcatcg
     3001 gccaccttct tcacctccgc ctcctcctcc ggcgtgaacg ggtcgagggc gtcgctgtcg
     3061 atgtcgatct ggcccaggat gtcgttcccc aggcggatga gcacgacgag ctcggagcgg
     3121 gtgaagaggt tgcaggcgat gtagttctcg cgggcgcgca cgtcgcccac gttctggtcg
     3181 agcccggtgg cgacggcggt gccgcacacc ccgtggccga cggcgatgcg ggtgtgctcg
     3241 gtctcccggc cactatgggc ctcgagcacg agctccccgt cctgcagcat ataaaggtag
     3301 acggaggtgt acgggctccc tgcggcgcgg atccggtcgg cggcgagttg gaggagtgcc
     3361 gtccgccccg cctcccgcgc aaaggcgccg cgcagcgcgg cgacgacctt ctcgccgtcg
     3421 aggaccggca tcagtcgccc cggacgccga gggtggcgtg ggtcacgacg tcgacgccgt
     3481 tgcgccggac gacgatctcg atcgtatccc ccgccttgtg ggcgcggagc acctcggtca
     3541 tccccatcag gtccttgacg tccttcccgt ccatccggac gatcacgtcc cccgcccgga
     3601 tcccggcctt ttccgcgggg ctgccggcgc ggacgccggt aagcgccacg ccgcccggtg
     3661 cctcgctcat gtcggggatg gtgccgaggt aggcgccgta gcccggggtg acggccgggg
     3721 tgctgtcggc cgcgacggcc ggctggggca cgtcgacgaa ggtgaggccg gtccgtcggt
     3781 cgcccacggc ggtggcgatg gcggccgcga agtcagccac gcgggccagg ccgtcggcgt
     3841 tgatcttctg ccagtcgtcg gtggggcggt ggtagtcgcc gtggaggtcg gtgaacaggt
     3901 gcagcaccgg ccggtgcgcg gcatagaacg acgactggtc gctggggccg tagccgtcac
     3961 catgctcctt cagttcgaag tgcgcggtgg cgttgagcga gtcgaggagc gcgggccact
     4021 cctgcgcggt ggcggagccg tacacgatga gcttcgagtc gcggagccgg cccaccatgt
     4081 cgaggttgat catcgcgacg gtgtggtcga gcggatagag cgggtgcttg acgtaccact
     4141 cgctgccgag gaggccctct tcctcgccgc tgaaggcgat gaagacgacg gtgcgcgccg
     4201 gtcggtgggc ggcgagccgc cgggcgatgg ccaccagcgc cgcgctcccc gaggcgttgt
     4261 cgtcggcgcc gttgtgcacc ttgccggtgc tgtcgggagc gagcgagccg aagccgccga
     4321 gcccgaggtg gtcgtagtgc gcgccgacca cgatgacctg atcctccagc accgggtccc
     4381 ggccggggag gacgccgatc acgttctcgc cgtgggcgcc cccgatgccg ctgtgctggg
     4441 ccgccggcgc ggtggggtcc accgtgaagc gctgcatcca cccgccgggc gcgggctgga
     4501 gccccgcggc ctggaaccgt cgggccaggt acgccgccgc ggtgtcggcg ccgggggtcc
     4561 cgatcttccg accctgcagg gcatcggagg caaggaactt gacgtcattg agcaggtcct
     4621 gggcctcgcc gccctgctgg gcgacgagcg gcgtggaggc gccgaaggcg agcagcagcg
     4681 ctgcggtcgt gaggatgcgc atggtcgacg gaagatagcg gcccacttcc actgccgccc
     4741 gtgacggata gaatccaccc gatgaacacc cgccacctcg ccctgatccc gacctgcatc
     4801 gccctcaacg tcgcgatggg caaggtggtc caggaactcg gtctcccgat ctacctcgac
     4861 gccgtcggca ccatcctggt cacggcgctc ctggggcccg cggcgggggt gctgaccggg
     4921 gcgctcagcc aggcgctcac cgccctcctc tcgggccaga tgatgtggct cgcgttcctc
     4981 ccgatccagg tgctgatcgc gatcgtggcg ggggtggcgg cgcagcgcgg cggattccgc
     5041 acggtcggct gggcggtcgg gtggggcgcc ctctgcggac tccttggcgg cgtctgctcg
     5101 gcggtcatct catacctgct gttcaagggg gtcaccgctc ccggcgtcac cgcgctcggc
     5161 gccctcttcc gctccctcgg gttctcgctc gagcgcgcgg tgaccgctgc cagcatctcg
     5221 accgacctcg tggacaaggg gatcgccttc gggctcgtgg ccgcggtgct gctcgcgctc
     5281 ccggcgcgga tggccagccg cttcccgagc gccgtccggg ccaccgggcg cggggcgtga
     5341 ccgcaggatc gccggcgtcg ccgatcggcc gggcccaccc gttcacgccg ctcacggcca
     5401 cgctcgccgt ggtgttcctc gcgttcctcc tgccggcgcc cgccgggccg gtcgtcctcg
     5461 cgctccttgc ggtggcctgc gccccgctcg tgggcgacgc cgccgcgctt tatccggcgc
     5521 tcatcatcgt cgtcccgctc tggctcctgc tcttcctggt gctgggggtg ctgggcgatg
     5581 caccgttcac ggcgctcgga ccggtgacgc tctccgtggc cgggacgcgc gaggcgctgg
     5641 cccagggagc gcggctcacc gcgatcgtcg tcccctcgct cctggcgctg cgcggattcc
     5701 gcgccgcacg cttcgtcgac gccgccgccg aacgggggtg gcccttctcc gccgcgtacc
     5761 tggtggcggc caccctccag accatcccgc ggctccggca ccgggtgatc ctgatccggg
     5821 atgcacaacg ctcgcgcggg ctcaaggtgc gcggctcgat cgtgcagcgg gtccgggcgc
     5881 tggtgccgct caccctgccg ctcatccttg gcgccctggc cgacgccgac gaccgggcgg
     5941 tggcgctcga gagccggggc gtcgccgcgg tggtggtgcc gggcgcacga cgcacccccc
     6001 tcgacccacc ggccgacagc atactcgacc ggacgatccg ctggggtgcg gtgcttgcgg
     6061 tcgtgctcgc ggccgcatgg cgggtcgtcc ggtgaccgtc cgcttcgacg gcgtcagctt
     6121 cacctacccg ggttcggcgg tgcccgcgct ccgcgacctg tcgctcacgc tcgcgggggg
     6181 ccaggtcacc tggctcttcg ggcggctcgg cgccggctcg agtacggcgc tcctcgtggc
     6241 gggcggattt gcgccgatcc tcaccggcgg ggagcggcgg ggcgcggtgg aggtggccgg
     6301 cctcgacccc ggcgatccgg ccacccgcgc ggccctggcc gggcgcgtgg cgtacgtatc
     6361 ggcgcagccc cacctgcagc tgtcggggct ggcggagacg gtgttcgagg aggtcgcgtt
     6421 cgcgccggcc aacctcgggt ggtcccgggc gcgcatcgcc gacttggtgc cccaggcgct
     6481 cgcgcggatc ggcgccgcgc acctggccgg gcgcgttccc agcgagctct ccggcgggga
     6541 actccagcgc gtggtcatcg cggcgatgct cgtgctggcg ccggaagtgt ggctgctcga
     6601 cgagccggcc tcggcgctcg acccgggcgc gcgcgcggcg gcatacgccc tcttccgcga
     6661 ggaggcgcgc cggggggccg cggtggtcat cgccaccgag gacgccgatg gtgcgctcga
     6721 ggccgccgac cggatcgtgg tgctcgaaaa cggcgccgtg gcccaggacg gcgtgcccgc
     6781 cgccgtcctt gccagcgacg cctgctggct cctcggcgcc ggcagcacca ccgtcggcga
     6841 actctggcgc gccgcgatgg agcgcgcgcc ggcgatggcg cgcggccagg tgccgctgac
     6901 cctcgccgcg gccgccgcgc gggtggcggg acgatgaaca tcgcctcggc acgcgacctc
     6961 cggttccgtt atggcgaccg ggagacgatc gcgggggtgt cgttcgacgt ggcgccgggg
     7021 gagggcgtcg cgctcctcgg acccaacggg gcggggaaga cgacgctcct ccggctgctg
     7081 atggcattcc tgcagccgcg gtcggggagc ctcgacgtgg ccggccggtc gacccggggg
     7141 ctcttccccg aggacctcgc cggcctggtc gggttcctct tccagcgccc cgaggaccag
     7201 ctcttccgcc gcaccgtgcg cgacgacgcc cgcttcgggc ccgagacgct cggctgggac
     7261 ccggcgcacg cggatgcagc ggtggacgcc gcgctccggg aactcgggct cgacgatgcg
     7321 gcccacgtcc atccgtacga cctgccgctg ccgcggcgcc gcctcgtggc gctcgccggc
     7381 gtgctggcgc tggagccgaa gctgctgctg ctcgacgagc cgaccgccac cctcgaccgc
     7441 acggcgcgca ggatggtcgt cggcgcactc agggcccggc gggagcgggg gatcgcggtc
     7501 atcgccgtca cccacgacat cggctttgcc gccgagctct gcgaccgcgc cctggcgctc
     7561 gatgcgggac ggatcgtggc cgacgggccg atcgccgacg tgctcgggcg gccgggggcg
     7621 gcgccgctgc cggtcggtgc cgagctcgtc cgccgggtcg gcgccactgc ccgctcgctc
     7681 cgatgggacg acctggtctc ggcccttgca agtcgttgcc gcgacctgca agagtgaata
     7741 gctttgggcg cggcatcggc gccgccccat atgagtgctc ccctctcagt cgtctccgat
     7801 ctatggaacg tcgtcgccct cctccgcgat cgtccgaacg cgaaggaaga gcataagacc
     7861 gcgttccgca ccctcgtgtc gaccctgggc gtgagcccgc tctccctcac cgccacgccc
     7921 gaggggctgg cgtatcaggg cgtgctcgtg ccggaggaga cggtcggcgc ggccgcgctc
     7981 acccggcaga tgctgcagca cggcgtgggc gagatcgagc ttcccgaggg gaccggtgcc
     8041 gcgccgctcc tgtccttgct tcgcgccctg gccgcaccca acggctccta ccggcacatc
     8101 caggagttca tcaccgccgt cggtgagccg ggggcgagcc ggttccggat ggcggccttc
     8161 gtggccccgc cgccggccgc gccggtcgtc tcggcgcggg cgtcgcgcgc ggcagcggcg
     8221 ccccccgcgc cgcgggtcac caccggttcg ccgccgcccc gcggatcggc cgagctggcg
     8281 ctcgggcccg gggtgatgtc gtcggaggag ggcgggatgc tgcacttcat gtcgtcgcag
     8341 cacgaggcca tcgcccggct ggatgagctc tccctcgcca tcgaggcggc gccgtccggc
     8401 gtcaacgtcg gccacttgct gagcgagctg gtcggcgccg ccgatgcggc ggcgcagaaa
     8461 tccgactggg aagggatcct gcgcgccgcc gtgcgggtga tcggcgccga gtcgaaggtg
     8521 acgtcgaagg acgagggccg gctgtacagc atctcgctca agcggctcct cccgcgccgg
     8581 acgctggacg agatcgcgcg cttcctgccg aagccggcgc tcaagcacga cgcgatgacg
     8641 gtgatgcagc ggatggggga gagcgccacc gacgtcctgc tcgacttcct ggccgccgcg
     8701 cccaccatcg aggagcggcg ccactacttc gacgcactgg tgacctcggc ccaggggtcc
     8761 gacatggtgg tccggacgct ggagcacggc gaatggttcg tgatccggaa cgtggccgag
     8821 ctctgcgggg agttgaagct cgagtccgca gtgccggtgc tggcgcggca cctgacccac
     8881 cccgacgaac gggtcaagcg cgccgccatc gtggcgctcg ccaagatcgg caccccggcc
     8941 accgtggagc cgctccggaa cgccctcaag gacgccgccc ccgcggtgcg gatgcaggcc
     9001 gtcatggtca tcgacgggaa gcagaaccgg gcgctcggcc tccggctggt gtcgatcctg
     9061 gaagaggaga ccgtgccgga ggtgctccgc gaagtgtgct attcggtggg gcggagtgga
     9121 tcgaacgaag gagtgcaggc gctcatccgg atggcgagcc ccggcgggaa gctcttcaac
     9181 aagaagccca ctcagcagcg tgtctgggcc atcgaagggc tccggatggc cggcggttcg
     9241 tcggcgcagg gggcgctcga gggactcttc caggacagcg acaaggaggt ccgcgaggcg
     9301 gcgcagaagg cgctctccgg gatgggcagg tagctatcag ctaacagcta tcagctaaca
     9361 gctaacagct aacagctaac agctatcagc tatcagtaac tgctgtgccg atggccaaga
     9421 agcctccgac gtcattgtcg gaggcttccg gtcattcagg actcccgtgc cagtcggctg
     9481 tccgctgata gccgatagct gatagctgat agctcacaca ggcacaaagc tcgtgtccgg
     9541 ccccacccgc cgggcaaagg ccgcgaggag tcgggccgcg cgatccaggt cctccagcgc
     9601 caccatctcg ttgggggagt gcatgtaccg gagcggcacg ctcaccaggc ccgtggcgac
     9661 gccgcggtgg gcggtgaaga tgttgtcggc gtcggtgtgg gtggcgcggg gtgccgcctc
     9721 cacggtgtag gggatcttct cccgctcggc tgcctcgacc agcaggctga acaccaccgg
     9781 attgaccgcc gagccgcggg agatgaccgg cccgccgccg aggcggaagt cgccgtgctt
     9841 gcgcttctcg atgccggggt agtcggtggc gtgggtcacg tcgaccgcga tggccacggt
     9901 ggcgtcgagc gtcgtggcgg aagtgcgggc accgccgccg gtgtagccga tctcctcctg
     9961 cgtcgtcgcg accgcggtca cgttggcctc aagcgcgccc cgcttcccgc tccccgctgt
    10021 ccccttgccc tttttcccct ttctcccttc tccc
//
LOCUS       DASUAP010000368         5438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_54027, whole genome shotgun sequence.
ACCESSION   DASUAP010000368 DASUAP010000000
VERSION     DASUAP010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54027"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VFU45_09920"
     CDS             complement(<1..1136)
                     /locus_tag="VFU45_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5359427.1"
                     /translation="MPSPHPSDTRKLAVLMTVAFVDMVGLVMIVPLLPFYATDFGASA
                     TTVGILISAFSVSQLVSAPLWGRLSDNRGRRPAIITGLLVSSGAYVIFGLAGSIAMLL
                     LSRLIQGTGGGTIGVVQAYVADASAPEDRAKALGWLSAVTSLGAVVGPALGSFLSDLW
                     GRHAPGFVSAGLCVLVALFARGYLEEPSSERRTGLQPAIPRSSRAALLGVLRHDDATS
                     RLIWLYTIAIGAFYGTVQIIPLLMADRFHVTERNIGYFVMYLGGMGVVARAGILGWMV
                     DRLREARLSQLGLVLLGAGLMTAAAARSYPVLFLSMTLMPLGTAFLFPCITGLLSRVV
                     ASHERGLYLGVQQTFGGVSRVAFPVGAGFLIDRWGTGTPFWISGA"
     gene            complement(1282..1683)
                     /locus_tag="VFU45_09925"
     CDS             complement(1282..1683)
                     /locus_tag="VFU45_09925"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU5359428.1"
                     /translation="MTIPPFETTFEVTPADIDERGHVNNVVYVRWVNDVATMHWRTGA
                     TPEQQADLAWVLLRHEIDYKDAALPGDRVRARTWVGHVEGLKFERFVELTRAADGKVL
                     AHSRTTWIPVDARTGRPRRVPAEVRERFSVG"
     gene            complement(1691..2182)
                     /locus_tag="VFU45_09930"
     CDS             complement(1691..2182)
                     /locus_tag="VFU45_09930"
                     /inference="COORDINATES: protein motif:HMM:NF039724.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6314 family protein"
                     /protein_id="HEU5359429.1"
                     /translation="MPTQVSTPHSLDRLWTRLMAVRRVVIDVSDAGTQGRSDAAMTGR
                     AEGSVEWLNGRPHEVTWREHGRWTEGPLAGTRFSSAYAWHRRPDGALSISHARFGVDR
                     PVALVDLVESGPGAWRSAAPHLCAEDQYAATLELEARVIVVTWTVTGPTTDYVLRARY
                     LPS"
     gene            2269..4170
                     /locus_tag="VFU45_09935"
     CDS             2269..4170
                     /locus_tag="VFU45_09935"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5359430.1"
                     /translation="MKIRHPFIGTALAASLGAAALAAQSTPPALQAVAGMVPATDVRT
                     AAGRPGPRYWQQRADYRIDVTLDPATDLVTGAETITYHNNSPDALPYLWMQVEQNICA
                     PNSVTNRLHQPPLVFQDATFDFSCQGFDGGDSLESVRVNGVAATHTLFGTTMRLDLAH
                     PLAPGAIATIAITWRFKVPPYGAGRMGHDGPLYELGQWYPRMCVYDDVKGWNHEPYIG
                     AGEFYLEYGSFKVNVTLPASYVVSATGRLLNPLETLTATERARLARARVSDTAIAIIS
                     ANEAGTRMTRPQMSGMMTWRYAADSVRDFAIAAAPNWRWDASGYDEILIHTYYRPNAS
                     LWPEANKMARHAIKYYSEQWLRYPYPHASAIEGPIEGMEYPMMTFDPSGPDRLDLAWT
                     VAHELGHQWTPMLIGSNERLYPWMDEGFNTFIDLGNAASYFAGTAYGDSIEVHPLHLY
                     PLHAIEGSEQPLITNPVESHDLFWTGYQKPALMMQTLRYQVLGKARFDDAFRDYLKTW
                     EFRHPTPADFFRLMRDESGRELGWFWNDWIYTVDRPDQAVTAISNADSGTSVTLENKG
                     TMQLPVYLKLSYADGSTETDTLPVEMWNLGPRYVYRVARGKRVVKAEIDPGSTLPDTD
                     RGNNVLTAP"
     gene            complement(4384..4863)
                     /locus_tag="VFU45_09940"
     CDS             complement(4384..4863)
                     /locus_tag="VFU45_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359431.1"
                     /translation="MTLRRWGVRVILGVGLLAACRPAGPSTADLARIESVWQAAADSA
                     TRSACADSLAHGRSRCTTVHGADTLFIWGDSAHRAAGFIRVHPVGRAGQDSAATALRE
                     QLAATLGPGVQCTVQVHGWSVGPASVMVVRRPDGGVVLVLGTHQQAAIVCPGLGDRE"
     gene            complement(4860..>5438)
                     /locus_tag="VFU45_09945"
     CDS             complement(4860..>5438)
                     /locus_tag="VFU45_09945"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HEU5359432.1"
                     /translation="WNWAGLEGQELEVWCHSNLDAIELLVNGASAGTRDVPRQGHVSW
                     KVPYAAGAIEARGMKDGKVVLTDRRETTGPAVSLRLVAWRTPLLADGEDTTVLRAEVV
                     DSAGRVVPTADASVTFDVSGTGRLIGVGNGDPSSHESDRGPTRRAFNGLCCAIVQASR
                     RSGEILVHATAPGLGAAATIVSTAPATPRPAA"
BASE COUNT          891 a         1907 c         1887 g          753 t
ORIGIN      
        1 gcacccgaga tccagaacgg ggtgccggtc ccccaccggt cgatgaggaa acccgcgccg
       61 acggggaacg ccacccggga gacgccgccg aaggtctgct gcacgccgag gtacaggccg
      121 cgttcgtggc tcgccaccac ccgtgaaagg agcccggtga tgcacgggaa gaggaatgcg
      181 gtgccgaggg gcatcagcgt catagaaagg aagagcaccg ggtagctgcg ggccgccgcc
      241 gcggtcatca ggccggcgcc gaggagcacg aggccgagct gcgaaaggcg ggcctcccgg
      301 agccggtcga ccatccatcc gaggatcccc gcccgtgcca cgacgcccat gccgccgagg
      361 tacatcacga agtatccgat gttgcgttcg gtcacatgga agcggtcggc catcaggagg
      421 gggatgatct gcaccgtgcc gtagaacgcg ccgatcgcga tcgtgtagag ccagatgagt
      481 cggctggtgg cgtcgtcgtg gcggaggacg ccgaggagtg cggcgcgact gctccgcggg
      541 atggcgggct ggaggccggt gcggcgttcg gaggacggtt cctcgaggta tccgcgggcg
      601 aagagcgcca cgaggacgca gaggccggcc gagacgaagc cgggggcgtg gcggccccag
      661 aggtcggaga ggaaggagcc gagcgccggt ccgaccaccg cgccaaggct ggtgacggcc
      721 gagagccagc cgagcgcctt ggctcggtcc tcgggcgcgc tggcatcggc cacgtaggcc
      781 tggaccacgc cgatggtgcc gccgccggtc ccctggatga ggcgggacag gaggagcatc
      841 gcgatcgagc cggcgaggcc gaagatgacg taggcgcccg aggagacgag gagtccggtg
      901 atgatcgcgg ggcgccggcc gcggttgtcg ctcaggcggc cccagagcgg ggccgagacc
      961 agctgggaca ccgagaacgc cgagatgagg atgcccaccg tcgtggcgct ggcgccgaag
     1021 tcggtggcgt agaaggggag gagtgggacg atcatcacca gccccaccat gtcgacgaac
     1081 gccaccgtca tcaggacggc gagcttgcgg gtgtcggatg ggtggggtga gggcatttgt
     1141 gtaagctatc agctatcagc tatcagctat cagaagaagg gtggggatct ttcacgagcc
     1201 ggccttgact acctgatgcc tctcgccacc gtcgcgaagc tgatagctga tagctgatag
     1261 ctgatagctg atagctgata gctaccctac cgaaaaccgc tcccgcacct ccgccggcac
     1321 gcgccggggc cggccggtgc gggcgtccac cgggatccac gtcgtccggg agtgcgcgag
     1381 caccttgccg tcggccgcgc gcgtcagctc cacgaaacgc tcgaacttga gtccttcgac
     1441 atggcccacc cacgtccggg cccggacccg gtcgccgggg agggccgcgt ccttgtagtc
     1501 gatctcgtgg cgcaggagca cccacgccag gtcggcctgc tgctccgggg tggcgccggt
     1561 gcgccagtgc atcgtggcga cgtcgttgac ccaccggacg tagaccacgt tgttgacgtg
     1621 accccgttca tcgatgtccg ccggggtcac ctcgaacgtg gtctcgaacg gggggatggt
     1681 cacgacaacg tcaggagggg aggtagcgcg cgcgcaggac gtagtcggtg gtgggacccg
     1741 tcaccgtcca ggtgacgacg atgacccggg cctcgagctc cagcgtggcc gcgtactggt
     1801 cctccgcgca gaggtgcggc gccgcggagc gccaggcgcc ggggccggac tcgaccaggt
     1861 cgaccagcgc caccggccgg tcgaccccga accgcgcgtg cgaaatggac aatgccccat
     1921 ccggccggcg atgccaggcg taggccgagg aaaagcgggt gccggcgagc ggcccctcgg
     1981 tccagcgacc gtgctcccgc caggtgactt cgtgcggccg gccgttgagc cattccacgc
     2041 tcccttcggc ccgtcccgtc attgctgcgt cgctgcgccc ctgcgtccca gcgtccgaca
     2101 cgtcgatcac gacccggcgc accgccatca ggcgggtcca gaggcgatcg agcgaatgcg
     2161 gggtggagac ctgggtcggc acggcacaga aagtactgtc cgccgagcga agccgcgagg
     2221 ggcggcgtat tatggagacg tccccagccg acatctgtgg aggccgtgat gaagatccgc
     2281 cacccgttca tcggtaccgc gctcgcggcg tcgctcggtg cggccgcgct cgccgcccaa
     2341 tccaccccgc ccgccctcca ggccgtggcc gggatggtcc ccgccaccga tgtccgcacc
     2401 gcggccggcc gcccgggccc ccgctactgg cagcagcgcg ccgattaccg gatcgacgtg
     2461 acgctcgatc ccgccaccga cctggtgacg ggcgccgaga cgatcaccta ccacaacaac
     2521 tcccccgacg cgctccccta cctctggatg caggtggagc agaacatctg cgcccccaac
     2581 agcgtcacca accggctgca ccagccgccc ctggtcttcc aggacgcgac cttcgacttc
     2641 tcctgccagg gcttcgacgg gggcgactcc ctcgagtcgg tgcgggtgaa cggcgtcgct
     2701 gcgacccaca ccctcttcgg caccacgatg cggctcgacc tggcgcaccc gctggcgccc
     2761 ggggccatcg ccacgatcgc catcacgtgg cgcttcaagg tgccgcccta cggcgccggc
     2821 cggatgggac acgacggccc gctctacgag ctgggccagt ggtatccgcg gatgtgcgtc
     2881 tatgacgatg tgaagggatg gaaccacgag ccgtacatcg gggcggggga gttctacctc
     2941 gaatacggga gcttcaaagt caacgtgacc ctcccggcga gctacgtcgt ctccgccacc
     3001 gggcgcctcc tcaacccgct cgagaccctg accgccacgg agcgcgcccg cctggcccgc
     3061 gcccgcgtga gcgacacggc catcgcgatc atctccgcca atgaggccgg cacgcggatg
     3121 acccggcccc agatgtccgg catgatgacc tggcgctacg ccgccgactc ggtgcgcgac
     3181 ttcgcgatcg ccgcggcgcc gaactggcgc tgggacgcga gcggctacga cgagatcctg
     3241 atccacacct actatcgccc gaacgcctcg ctctggcccg aagcgaacaa gatggcccgg
     3301 cacgccatca agtactacag cgaacagtgg ctccggtatc cctatcccca cgcctcggcc
     3361 atcgaagggc cgatcgaggg gatggaatac ccgatgatga ccttcgaccc gtccggcccc
     3421 gaccggctcg acctcgcgtg gacggtggcc cacgagctgg gccaccagtg gaccccgatg
     3481 ctcatcggca gcaacgagcg gctctacccg tggatggacg aggggttcaa caccttcatc
     3541 gacctcggca acgcggcgag ctacttcgca ggcacggcat acggcgactc gatcgaggtc
     3601 cacccgctcc acctctaccc gctccacgcc atcgagggct cggagcagcc gctcatcacc
     3661 aacccggtgg agagccacga cctcttctgg accggctacc agaagccggc cctgatgatg
     3721 cagaccctcc gctaccaggt cctcggcaag gcgcgcttcg acgacgcctt ccgcgattac
     3781 ctgaagacct gggagttcag gcacccgact ccagcggact tcttccgcct gatgcgcgac
     3841 gagagcggtc gcgaactcgg ctggttctgg aacgactgga tctacaccgt ggaccgcccg
     3901 gaccaggccg tcacggccat ctcgaacgcc gactcgggga catcggtgac gctcgagaac
     3961 aagggcacga tgcagctccc ggtgtacctc aagctcagct atgccgacgg ttcgaccgag
     4021 acggacacgc tcccggtcga gatgtggaac ctggggccgc gatacgtgta ccgggtggcg
     4081 cggggaaagc gggtggtgaa ggcggagatc gacccgggga gcacgctccc cgacaccgac
     4141 cggggcaaca acgtcctgac cgcgccgtga gccggggatc cggtccggag gcccgatcgc
     4201 cttagctcga cgaggccccg tgtggcgtgg gaccacggag ggcgcggagg ttcacggagg
     4261 accaatgcgt gtgtggcggt gcgcatggag ttctgtgcgc cagaccacgg cagatgcagt
     4321 tctccgtgtc tctccgtgga ctccgtgccc tccgtgatcc cacactgtcc ggacgtggat
     4381 ctgctattcc cggtcgccga ggccggggca gacgatggcc gcctgctgat gggtgccgag
     4441 cacgagcacc acgccgccat cggggcgcct gaccaccatt accgacgccg ggccgaccga
     4501 ccagccgtgg acctgcacgg tgcattgaac gccggggccg agcgtggccg cgagctgctc
     4561 gcgaagcgcg gtggcggcgc tgtcctgccc ggcgcgcccg accgggtgga cgcggatgaa
     4621 tccggcggcg cggtgggcgg agtcgcccca gatgaagagg gtgtcggcgc cgtggacggt
     4681 ggtgcaccgg ctgcgtccgt gggccaggct gtcggcgcag gccgatcgcg tggcggaatc
     4741 cgcggcggcc tgccagaccg attcgatcct ggccaggtct gcggtcgatg gcccggcagg
     4801 ccggcacgcg gccagaaggc cgacgccgag gatgacccgg accccccatc gtcggagcgt
     4861 catgccgcag gccggggcgt ggccggtgcc gtcgagacga tcgtcgccgc ggcgcccagg
     4921 cccggcgcgg tggcatggac gaggatctca cccgagcgcc tgctcgcctg gacgatggcg
     4981 cagcagaggc cgttgaacgc ccgccgggtc ggcccccggt cggactcgtg gctcgagggg
     5041 tcgccgttcc cgacgccgat gagccgtccc gttcccgaca catcgaaggt cacgctcgca
     5101 tccgccgtcg gcaccacccg tcccgccgag tccaccacct cggcgcggag caccgtggtg
     5161 tcctcgccgt cggcaaggag cggcgtccgc cacgccacca ggcggaggct cacggccggc
     5221 cccgtcgtct cccgccggtc ggtgaggacc accttgccgt ccttcattcc ccgcgcttcg
     5281 atggctccgg ccgcgtaggg cactttccag ctgacgtgcc cctggcgcgg cacatcccgg
     5341 gtgccggcgc tcgcaccgtt cacgaggagc tcgatcgcgt cgaggttcga atggcaccag
     5401 acctcgagct cctgtccttc caggccggcc cagttcca
//
LOCUS       DASUAP010000369         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1048798, whole genome shotgun sequence.
ACCESSION   DASUAP010000369 DASUAP010000000
VERSION     DASUAP010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048798"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..827)
                     /locus_tag="VFU45_09950"
     CDS             complement(6..827)
                     /locus_tag="VFU45_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359433.1"
                     /translation="MTAALLLAAPLHAQTMREFQASHRRGGARSLAVRVDFNAGSLRL
                     AAARPGDLYRMRLAFDAERFAPVAQYSAADGRLTLGTSPVGATGFRVVGRRQLAQQAS
                     VALAPDVPLTLDVNIGAGLGDIDLGGLQISSASIHTAASRTTVAVSTPNPGRCSTLDL
                     DAGASELSTDRLGNARCANIRFQGGVGKVTLDLTGAWTDALRVQAHMALGELHLLLPT
                     DAGVEIMLDRTLTSFQPVGFTRQGTTYTSTNFARAQRTIRIDVSTAVGGIVTEWK"
     gene            complement(842..2416)
                     /locus_tag="VFU45_09955"
     CDS             complement(842..2416)
                     /locus_tag="VFU45_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359434.1"
                     /translation="MRRPSLLLLAALLLTPSGPLLARGGHLRASGPFAAPITFLRQLL
                     HVGPGPTVRDHEVAVSSDSRTLTLRMANGPDLSITLTQGRLLINGATAGHYAAGGDVE
                     GAWQDLLSDAARLPTALAVGRLRNWHPDDLVGDDAAAAEAIEARLAGLVASPGASTEP
                     QAIPAAAPGGLTVDLSDLSDLGRLEPQLYRASQLSPADLRITVPGGQARLGHFSIGSG
                     QTVDGHLLVVRGDADIFGTLRGNLATVDGDIVMHPGAVVTGSVLAVGGQVRETGGDIH
                     GSVLSLDDAFPAPHASAPAVVRPWQRMLTGSAGVLGVFVTLAVVGFGLVAFARPQLEV
                     VGDTVAHSFGRALVVGLLGQVLLVPTFGMMVVGLALTLVGILLVPFAVAVYILLAVVA
                     ILGGLLGAAHAMGETWTRRRLAGGARALGMNSYSYLLVGLGFLMALWACDVVFGWVPV
                     AGTIIASLAVLVSWLVATAGFGAALLSRAGLRDAFTGRIIPPEALTDEYLWATPQFGV
                     PAVQRPASTRTPPAKP"
     gene            complement(2403..3005)
                     /locus_tag="VFU45_09960"
     CDS             complement(2403..3005)
                     /locus_tag="VFU45_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359435.1"
                     /translation="MTELTPPHLTPEDLDLWLAGELPPGRLVHLDDCPDCFALAHAER
                     RVVALIAALPVIAPHAGLADRVMAAVRVPPAPTRAPWRVLAHPSLLGPRWRALVAASV
                     AVVAGASASVAWSLAHPEAFPAASAWVAGEASQWFWVTVRTLASNVTEQPWYGAARSL
                     LGSPARAALVSAFGILLYASGIVAMRRLLTPAARGPHAQA"
BASE COUNT          455 a         1069 c         1213 g          357 t
ORIGIN      
        1 tcctactatt tccactccgt cacgatccct cccaccgcgg tcgatacgtc gatccggatg
       61 gtccgctgcg ccctggcgaa gttcgtggat gtgtaggtgg tcccctgccg ggtgaagccg
      121 accggctgga agctggtgag cgtccggtcg agcatgatct cgacgccggc atccgtgggg
      181 aggaggaggt ggagttcgcc gagcgccatg tgggcctgga cgcggagcgc gtcggtccac
      241 gcgccggtca ggtcgagcgt caccttgccg acgccgccct ggaaccggat gttggcgcag
      301 cgggcgttgc ccaggcggtc ggtgctgagc tccgaggccc cggcgtcgag gtccagggtg
      361 ctgcagcgtc cggggttcgg cgtgctcacc gcgacggtcg ttcggctcgc cgcggtgtgg
      421 atcgaggcgg aggagatctg gaggccgccc aggtcgatgt cgccgagccc ggcgccgatg
      481 ttgacgtcga gcgtgagcgg cacgtcggga gcaagcgcca ccgacgcctg ttgggccagc
      541 tggcgccggc cgaccacgcg gaatccggtc gcccccacgg ggctggtgcc gagcgtcagg
      601 cggccgtccg cggcggagta ctgtgccacc ggggcgaacc gctccgcgtc gaacgccagg
      661 cgcatccggt agaggtcgcc cgggcgggcc gcggcgaggc gcagcgaccc cgcgttgaag
      721 tcgacccgga ccgcgagcga gcgggcacct ccccgccgat ggctggcctg gaattcccgc
      781 atcgtctggg cgtggagcgg cgccgccagc agcagggccg cggtcacgga ggtccagggt
      841 ctcacggctt ggccggcggg gtgcgggtgg aggcggggcg ctgcaccgcc gggacgccga
      901 actgcggggt ggcccagagg tattcgtcgg tgagcgcctc gggcgggatg atgcgcccgg
      961 tgaacgcgtc gcgcaggccc gcgcgggaga ggagcgccgc gccgaacccg gcggtggcca
     1021 ccagccacga cacgaggacc gcgaggctcg cgatgatcgt gcctgccacc ggcacccagc
     1081 cgaacaccac gtcgcaggcc cagagcgcca tcaggaaccc gaggcccacg agcaggtagc
     1141 tgtagctgtt catcccgagg gcccgtgccc cgcccgccag gcggcggcgg gtccacgtct
     1201 cgcccatcgc gtgggcggcg ccgaggaggc cgccgaggat cgccaccacg gcgaggagga
     1261 tgtagaccgc cacggcaaag ggcacgagca ggatcccgac cagcgtgagc gcgagcccca
     1321 ccaccatcat gccgaaggtc ggcaccagga gcacctggcc caggagcccg acgacgagcg
     1381 cgcgtccgaa ggaatgcgcg acggtgtcgc cgacgacctc gagctgcggc cgggcaaagg
     1441 cgacaagccc gaagccgacg acggcgagcg tcacgaacac acccagcacc ccggccgacc
     1501 cggtcagcat ccgctgccag gggcggacca cggcgggtgc ggatgcgtgg ggtgcgggga
     1561 aggcgtcgtc gagcgagagc acgctgccgt ggatgtcacc gccggtctcg cgcacctgtc
     1621 cgccgaccgc cagcacgctg ccggtgacga ccgcgccggg atgcatcacg atgtcgccgt
     1681 cgacggtggc gaggttgccg cgcagcgtgc cgaagatgtc cgcgtcgccc cggaccacga
     1741 ggagatgccc gtccaccgtc tggcccgagc cgatgctgaa gtgccccagg cgggcctgcc
     1801 cgccgggcac cgtgatgcga aggtccgcgg gagacagctg gctggcgcgg tagagctggg
     1861 gctccaggcg gccgaggtcg gagaggtcgc tcaggtccac cgtgagtccc cccggcgctg
     1921 ccgccggaat ggcctggggc tccgtcgacg ccccggggct cgccacgagg ccggccaagc
     1981 gtgcctcgat cgcctcggcg gcggcggcgt catcgccgac caggtcgtcg gggtgccagt
     2041 tgcgcagccg gccgacggca agggcggtgg gaaggcgggc ggcgtcgctc aggaggtcct
     2101 gccaggcgcc ttcgacatcg ccgccggcgg cgtagtggcc ggccgtggcg ccgttgatga
     2161 ggagccggcc ctgggtgagc gtgatggaga ggtcggggcc gttggccatc cggagcgtca
     2221 gcgtgcggct gtcggaggag acggccacct cgtggtcgcg gaccgtgggc ccgggcccca
     2281 cgtggagcag ctgccgcagg aaggtgatgg gcgccgcgaa cggcccggac gcgcggaggt
     2341 gcccgccccg ggccagcaac gggccggacg gggtgaggag cagggcggcg aggaggagga
     2401 ggctaggcct gcgcatgggg cccccgggcc gcgggcgtga gcaagcgccg catcgcgacg
     2461 atcccgctcg catagagcag gatgccgaag gcggagacga gcgccgcacg agccggggag
     2521 cccaggagcg accgggccgc cccgtaccag ggctgctcgg tgacgttgga ggccagggtc
     2581 cgcaccgtga cccagaacca ctggctggcc tcacccgcca cccacgcgct ggcggcgggg
     2641 aacgcctcgg gatgagcgag gctccacgcc acgctcgccg atgcgcccgc cacgaccgcc
     2701 accgacgccg ccacgagggc ccgccagcgg ggcccgagca gcgacggatg cgccagcacc
     2761 cgccacggtg cgcgggtcgg cgccggcggc acccgcaccg ccgccatgac gcgatcggcg
     2821 aggccggcgt gcggcgcgat cacgggcaat gccgcgatca gggccaccac gcgccgctcg
     2881 gcgtgcgcca gggcgaagca gtcggggcag tcgtccaggt gcaccagacg ccccggcggg
     2941 agctcgccgg caagccaaag atcgagatct tcaggcgtga ggtgcggggg tgtgagttcg
     3001 gtcataactg ggctctccta cgagcggtct cgacggctgg tttcagcgct tcaggtccga
     3061 caggtactcg cgtaactcgt tgcgggcccg gtgg
//
LOCUS       DASUAP010000370         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_380753, whole genome shotgun sequence.
ACCESSION   DASUAP010000370 DASUAP010000000
VERSION     DASUAP010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380753"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2031
                     /locus_tag="VFU45_09965"
     CDS             <1..>2031
                     /locus_tag="VFU45_09965"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU5359436.1"
                     /translation="PYVIMMPPPNVTAQLHMGHGLNNTIQDVLVRVARMQGRETLWLP
                     GTDHAGIATQNVVERLLAKEGKTRFDVGREAFVRRVWEYVDQTGATILDQLKAIGSSC
                     DWGRTYFTLDEGLSRAVREVFVLLYEKGLVYRGHYIINWCPRCLTALSNEEAEKDDVD
                     GKIWHLRYPLVPKAGGPGASLTSTLAMPAARLTTGDPHASAETIVVATTRPETMLGDT
                     AVAVHPDDERYRHLIGRMIRLPLTDREIPIVADAAVDPAFGSGAVKVTPAHDPADFEI
                     GRRHHLPTIDVMTPDARMADTVPERFRGLDRFDARKRVVAEFEQLGLLVKVDDHRHAV
                     GHCYRCDTVVEPRLSDQWFVRMAPLAQPALQAYRDGKLTFIPERRGEEYAQWMENIRD
                     WCISRQLWWGHRIPVWYCDGCGQMTVSRSEVEDCPSCRGPVRQDEDVLDTWFSSWLVP
                     FSALGWPVETADIKRFYPGTTLVTAPEILFFWVARMVMAGLEFRGELPFSTVYLHGTV
                     RDTQHRKMSKSLGNGIDPLDVVATYGADALRYTVVSGMAVGTDLILDPKDLELSFAAG
                     RNFANKLWNAGRFLLGNLDGTPRPIAGAYRNAVRKDELTLADRWIIARCEEAVGAATE
                     AIEKYRLNDAASAVYHFLWSDFADWYVELIKPRLYGDRPGGDVARAVAYRVF"
BASE COUNT          319 a          717 c          696 g          299 t
ORIGIN      
        1 cccgtacgtc atcatgatgc cgccgcccaa cgtgaccgcc cagctccaca tgggccacgg
       61 gctcaacaac accatccagg acgtgctggt gcgagtggcg cggatgcagg gacgggagac
      121 cctctggctc ccgggcaccg accacgcggg gatcgcaacg cagaacgtcg tcgagcgcct
      181 cctggccaag gaggggaaga cccgcttcga cgtcgggcgc gaggcattcg tccgccgggt
      241 gtgggagtac gtggaccaga ccggcgccac catcctcgac cagctcaagg ccatcgggtc
      301 gagctgcgac tggggccgca cctacttcac cctcgacgaa gggctctcgc gcgccgtgcg
      361 ggaggtgttc gtcctcttgt atgagaaggg gctcgtctac cgcgggcact acatcatcaa
      421 ctggtgtccc cgctgcctga ccgcgctgtc caacgaagaa gccgagaagg acgacgtcga
      481 cgggaagatc tggcacctgc gctatccgct ggtcccgaag gcgggaggcc cgggcgcgag
      541 cctgacctcc acgctcgcga tgccggcggc acggctcacc acgggcgacc cgcacgcgtc
      601 ggctgaaacg atcgtcgtgg ccaccacccg ccccgaaacg atgctgggcg acacggcggt
      661 ggcggtccac cccgacgacg agcgttaccg ccacctcatc ggccggatga tccggctccc
      721 gctcaccgac cgggagatcc cgatcgtcgc cgatgcggcg gtggatccgg cgttcgggag
      781 cggggcggtc aaggtgacgc cggcccacga ccccgccgac ttcgagatcg gccggcgcca
      841 ccacctcccc accatcgacg tgatgacccc cgacgcccgg atggccgaca cggtgcccga
      901 gcgcttccgc gggctggacc ggttcgacgc ccggaagcgg gtggttgcgg agttcgagca
      961 gctgggcctg ctggtcaagg tggacgacca ccgccatgcg gtgggccact gctaccgctg
     1021 cgacacggtg gtggagccga ggctcagcga ccagtggttc gtgcggatgg cgccgctggc
     1081 ccagccggcg ctccaggcgt atcgcgacgg gaagctcacg ttcatccccg agcgccgcgg
     1141 cgaggagtac gcccagtgga tggagaacat ccgcgactgg tgcatctccc ggcagctctg
     1201 gtggggccac cggatcccgg tgtggtactg cgatggctgc ggccagatga ccgtcagccg
     1261 gagcgaggtg gaggactgcc cttcctgccg cggccccgtc cgtcaggacg aggacgtgct
     1321 cgacacctgg ttctccagct ggctggtccc cttctccgcc ctcggctggc cggtcgagac
     1381 cgcggacatc aagcgcttct accccggcac cacgctcgtc accgcgcccg agatcctctt
     1441 cttctgggtg gcgcggatgg tgatggcggg gctcgagttc cgcggcgagc tcccgttcag
     1501 taccgtgtat ctccacggca ccgtgcgcga cacccagcac cgcaagatgt cgaagtcgct
     1561 ggggaacggg atcgatccgc tggacgtggt cgcgacctac ggcgccgacg cgctccgcta
     1621 cacggtggtc tcggggatgg cggtcggcac cgacctgatc ctcgacccga aggacctgga
     1681 gctgtcgttc gcggcgggac ggaacttcgc caacaagctc tggaacgccg ggcgcttcct
     1741 gctcggcaac ctcgacggca cgccccgccc cattgccggg gcgtaccgga acgcggtccg
     1801 gaaggacgag ctcaccctcg ccgatcggtg gatcatcgcg cgctgcgagg aggcggtcgg
     1861 cgccgccacc gaggccatcg agaagtaccg gctcaatgat gccgcgtcgg ccgtgtatca
     1921 cttcctgtgg agcgacttcg ccgactggta cgtggagctc atcaagccgc gcctctacgg
     1981 cgaccgcccc ggcggcgacg tggcgcgcgc ggtggcctac cgggtcttcg a
//
LOCUS       DASUAP010000371         4480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_925991, whole genome shotgun sequence.
ACCESSION   DASUAP010000371 DASUAP010000000
VERSION     DASUAP010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925991"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFU45_09970"
     CDS             complement(<1..247)
                     /locus_tag="VFU45_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359437.1"
                     /translation="MRRYMTVGLLALAALAPARRAAAQDSVIVINPDAPVDQAAERPG
                     FPPGLVQRLLAFYNDSATTRLEGEVVLPRGARMTGAVA"
     gene            complement(426..1778)
                     /locus_tag="VFU45_09975"
     CDS             complement(426..1778)
                     /locus_tag="VFU45_09975"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5359438.1"
                     /translation="MPTILLIDDEANIRRMLGALLRSEGFDVAEAGNGNAALLAVDAV
                     DPDAVFLDLMMPPGPDGLATLAALRERGVDAPVIMMSGKAQLSDAVRATQLGAFQFLE
                     KPLSPESALVTLRSALELRRTQVENRTLRAALVLDSELVGSSAALGRVRSLVAQVAPT
                     DARVLITGESGTGKELVALAIHRASRRSRQAFIPVNCAAIPRDLVESELFGHEKGAFT
                     GASDRRLGRFSLADRGTIFLDEVGDLHPEAQAKLLRVLETGELQRLGAERGERVDVRV
                     LAATNKKLDQAVTTGAFREDLYFRLNVFPIELPPLRDRAEDIPALVSHFTHRLRPRQP
                     PAFSAEALEALRRYSWPGNIRELANVVERLLILGGGGPEIGLEAVRQVIPATGAPMAA
                     PAAAAAGAGLPLAESLDAYERELIGAAIARAGGNVAEAARQLRTDRANLYRRMRRLGL
                     "
     gene            complement(1815..3032)
                     /locus_tag="VFU45_09980"
     CDS             complement(1815..3032)
                     /locus_tag="VFU45_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5359439.1"
                     /translation="MTLTFRQRIFAVLLVLGAVPTTLVLVGWALTLRRGDSGQVNRAA
                     VAEIGRTGRTLLETIDSTKLTHTERVALAAHARSMNTALSRVQQYEAYSRLMSAALTV
                     VILVLGGLILYGGARLAGHLSRQLSRPIDELVGWTGHILRREPLPPDQARKGAPEFTA
                     LRTALRELSDRIGDARERELEAERLRSFREVARRVAHEMKNPLTPMRFAVTQLQRSAG
                     EGQREALEVLQAESARLEQLAREFAEFGRLPEGPPADVDLNELLAELVRTSVPPGADA
                     RIKADPATPRVTGHYDPLRRAFSNILRNAVEATGGRGPVRVSVAPADGGATVRISDRG
                     PGVRAADRQRIFQPYVTTKSEGTGLGLAMVRQTVEAHGGRVWVEDTPGGGATFVVELP
                     RERPAAASGGRAE"
     gene            3203..>4480
                     /locus_tag="VFU45_09985"
     CDS             3203..>4480
                     /locus_tag="VFU45_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5359440.1"
                     /translation="MAQCPRPSTHDSGPTTHDPAFSPSVSSDSTYIYPALCQGPTLDN
                     WHLAETVEERARSHPARECLVAGLRRWTYGQVRDQSGALAAALADLGLVPGERIALLL
                     PNRPEWVIGFLAAARLGLTVVPLDPRLSFHELKYLLRHSEVVAALTVAGYGDQDFLDL
                     FDELLPELPDLHYVITVGVADLWADERVYRYEDLVARGAGRLSPVAPVDPERMPLAVV
                     YTSGTTAKPKGVVLSHRALLEATRGTGEALELRGDDRVLAAVPFSHVFGTHIALQCLQ
                     YGVTMLLLETFEPGAALALMMAERATVCHGVPTMFALLMRDPSFDADRLSGLRTGILA
                     GASVAPELVGQIRRWCDVQIAYGLTETGPTVSVTRFTDPPEKRANTVGRPVRGVEVRI
                     IDVSPGRSGEEAVGELAVKGPDLMLGYHRMPGET"
BASE COUNT          603 a         1617 c         1598 g          662 t
ORIGIN      
        1 ccgccacggc gccggtcatc cgcgccccgc ggggcagcac cacctcgccc tcgaggcggg
       61 tggtggcgct gtcgttgtag aaggcgagca ggcgctggac cagccccggc ggaaagccgg
      121 ggcgttcggc cgcctggtcc accggcgcgt ccggattgat cacgatgacg ctgtcctgcg
      181 ccgcggcccg cctggccggt gccaacgccg ccagggccag cagcccgacc gtcatgtatc
      241 gtcgcattca ctcccctcgg catggcgtga cctcagcccc acccactccc attcaccatt
      301 taccgttcac cgttcaccat tcaccgctca ccccttaccg ctcaccgttc accgctcacc
      361 ccttaccgct caccgttcac cgttcaccgc tcacccctta ccgctcaccg ctcaccgctc
      421 accgcttaaa gtcccagccg ccgcatccgc cgatacaggt tcgcccggtc ggtgcggagc
      481 tggcgggcgg cctcggccac gttgccgccg gcccgggcga tggccgcacc gatcagctcc
      541 cgctcgtagg cgtccagcga ctcggcgagc ggaagcccgg cgcctgccgc ggcggcggcc
      601 ggagcggcca tcggagcccc ggtggccggg atgacctgtc gcaccgcctc gagcccgatc
      661 tccgggccgc cgccacccag gatgagcagt cgctccacga cgttggcgag ctcccggatg
      721 ttgccgggcc agctgtaccg ccgcagcgct tccagggcct ccgcactgaa cgccggcggc
      781 tgccgcggcc ggagccgatg cgtgaagtgg ctcaccagcg ccgggatgtc ctcggcacgg
      841 tcccgcaacg gcggcagctc gatggggaag acgttgagcc ggaagtagag gtcctcccgg
      901 aaggcgcccg tggtcaccgc ctggtcgagc ttcttgttgg tggccgccag cacccggacg
      961 tccacccgct cgccccgctc ggcgccgagt cgctgcaatt cgccggtctc gaggacgcgc
     1021 aggagcttcg cctgcgcctc ggggtggagg tcgcccacct cgtcgaggaa gatcgtgccg
     1081 cggtcggcca gcgagaaccg cccgagccgg cgatcgctgg cgccggtgaa ggcgcccttt
     1141 tcgtggccga agagctccga ctccaccagg tcgcggggga tcgccgcgca gttgaccggg
     1201 atgaacgcct ggcgcgaccg gcggctggcc cggtggatgg cgagcgccac caattccttg
     1261 ccggtgccgg actccccggt gatgaggacg cgcgcgtcgg tgggcgccac ctgcgccacc
     1321 agcgaccgca cccgcccgag cgccgcgctc gacccgacga gctcgctgtc gagcacgagc
     1381 gccgcgcgca acgtccggtt ctccacctgg gtgcgccgga gttcgagcgc cgagcggagc
     1441 gtcaccagcg ccgattcggg ggagagcggc ttctccagga actggaaggc ccccagctgg
     1501 gtggcgcgca ctgcatcgga gagctgggcc ttgccgctca tcatgatgac cggcgcgtcc
     1561 actccccgct cccggagcgc cgcgagcgtg gccagcccgt ccgggccggg cggcatcatc
     1621 aggtcgagga acaccgcgtc ggggtccacg gcgtccaccg cgagcagcgc ggcgttcccg
     1681 ttgcccgcct cggccacgtc gaacccctcg gagcggagca gggccccgag catccggcgg
     1741 atgttcgcct cgtcgtcgat cagcaggatc gtcggcatct ggactcggtc gtggaggtgg
     1801 tcacgggtcc cgcgtcattc ggcgcgcccg ccgctcgctg ccgccggccg ctcccgcggc
     1861 agttcgacga cgaacgtcgc tccgccgccg ggcgtgtcct ccacccagac gcggccgccg
     1921 tgcgcctcga cggtctggcg caccatcgcg aggccaagcc cggtcccctc ggacttggtg
     1981 gtgacgtacg gctggaagat ccgctggcgg tccgcggcgc ggacgccggg gccgcggtcg
     2041 gagatccgca ccgtggcgcc gccgtcggcg ggcgccaccg acacccgcac cggcccgcgt
     2101 ccaccggtcg cctcgacggc gttccgcagg atgttgctga acgcgcgccg cagggggtcg
     2161 taatgtccgg tgacacgcgg ggtcgccgga tcggccttga tccgggcgtc cgcgcctggc
     2221 ggcacgctgg tgcgcaccaa ctcggccagc agctcgttca ggtccacatc ggcggggggc
     2281 ccctcgggca ggcggccgaa ctccgcgaat tcccgggcca gctgctccag ccgggccgac
     2341 tcggcctgca gcacctcgag cgcttcccgc tggccctcgc cggcggaacg ctggagctgg
     2401 gtcacggcaa agcgcatcgg cgtgagcgga ttcttcatct cgtgcgccac ccgccgggcc
     2461 acttcccgga agctccgcag ccgttcggct tccagctcgc gctcgcgggc gtcgccgatc
     2521 cggtcggaca attcgcgcag tgccgtgcgg agtgcggtga actccggggc gcccttccgc
     2581 gcctggtcgg gcggcagcgg ctcacgtcgg aggatgtggc cggtccaccc gaccagctcg
     2641 tcgatcggcc ggctgagctg ccgggagaga tgccccgcca gccgcgcgcc gccgtagagg
     2701 atgaggccgc cgagcaccag gatgaccacg gtgagggcgg cgctcatcag ccggctgtat
     2761 gcctcgtact gctggacccg gctcagggcc gtgttcatcg accgcgcgtg ggctgccagc
     2821 gcgacccgtt cggtgtgcgt cagcttggtc gagtcgatgg tctccaggag cgtgcgcccg
     2881 gtgcgcccga tctccgccac cgccgcccgg ttgacctgcc cggaatcgcc gcgacggagc
     2941 gtgagcgccc acccgaccag caccagcgtc gtcgggacag cgccgagcac cagcagtacc
     3001 gcgaagatcc gctgacggaa cgtcagggtc attctcgtgc tccaagtgac cgtggcccaa
     3061 ggactttgca atgtaccggc ccccggcggg taatgcgagt cggaatgaca cggggtgaaa
     3121 gagggtcgcg ggtcacgggt cgcgggtcgt gagtcctgaa ggatcgtacg ggtcgtcatt
     3181 ggcggcgcgt cgaatcgcgt acatggcaca gtgcccaagg ccgtcgaccc acgactcggg
     3241 acccacgacc cacgacccgg ctttcagccc ttccgtatcc tcagattcca catacattta
     3301 ccccgccttg tgccaagggc cgacgttgga caactggcat cttgccgaga cggtggagga
     3361 gcgggcccga agtcacccgg cacgtgagtg tctggtggcc gggcttcgac gctggaccta
     3421 cggacaggtc cgcgaccagt cgggggcgtt ggctgcggcg ttggcggatc tcgggctggt
     3481 gccgggtgag cgcatcgcgc tgttgctccc gaaccgtccg gagtgggtca tcgggttcct
     3541 ggcggcggcg cggctcggcc tgacggtggt gccgctcgac ccgcggctca gcttccatga
     3601 gctcaagtac ctgctgcggc acagcgaggt ggtggcggcg ctcaccgtgg cgggatacgg
     3661 cgaccaggac ttcctcgacc tgttcgacga gctgctcccg gaactgcccg acctgcatta
     3721 cgtcatcacg gtcggtgtcg ccgatctctg ggccgatgag cgggtgtacc gctacgagga
     3781 cctggtggcg cggggggcgg gccggctgtc gccggtggcg ccggtggacc ccgagaggat
     3841 gcccctcgcg gtggtgtaca cgtcggggac gaccgcgaag ccgaagggcg tggtgctctc
     3901 ccaccgggcg ttgctcgaag cgacccgtgg cacgggagag gcgctcgagc tgcgcgggga
     3961 cgaccgggtg ctggcggcgg tgccgttctc ccacgtcttc gggacacaca tcgcgctgca
     4021 gtgcctgcag tacggcgtga cgatgctcct gctggagacg ttcgagccgg gcgccgcgct
     4081 ggcgctgatg atggcggagc gggccacggt gtgccacggc gtgccgacga tgttcgccct
     4141 cctgatgcgc gacccgtcgt tcgacgccga ccggctgtcg ggcctgcgca ccgggatcct
     4201 ggcgggggcg agcgtggccc cggagctggt cgggcagatc cggcggtggt gcgacgtgca
     4261 gatcgcctac gggctcaccg agaccgggcc gacggtcagc gttacccggt tcaccgaccc
     4321 gccggagaag cgcgccaata cggtgggacg cccggtccgc ggggtcgagg tccgcatcat
     4381 cgacgtgagc cccgggcggt ccggggaaga ggcggtcggg gagctggcgg tgaagggccc
     4441 cgacctgatg ctgggttatc accgtatgcc gggggagacg
//
LOCUS       DASUAP010000372         4437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_912647, whole genome shotgun sequence.
ACCESSION   DASUAP010000372 DASUAP010000000
VERSION     DASUAP010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912647"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFU45_09990"
     CDS             <1..798
                     /locus_tag="VFU45_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359441.1"
                     /translation="LAGAAEALRALLMLQLGAEPEGITDALRQALAGYADRLSSGDLL
                     RMLKIVADQEGNVRRSGNPRLVVETTLLRWAMMDRTVDLERVLAQGGGAPAAPPAARA
                     APARSVPSPAPAASPPPAAAGAGPAPVPDTAVTSAPFSLEGVRSVWDEVVEAAGREKK
                     MLGIALAATRPVAADRPVVTLELTEENPMHAEALERLRGSVQALLGARLGSAVTIQVR
                     PPAGGGAPARPKRMTEAGNRAERLSKLRGKDPALDAAASELDLEVME"
     gene            869..1171
                     /locus_tag="VFU45_09995"
     CDS             869..1171
                     /locus_tag="VFU45_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU5359442.1"
                     /translation="MADFQQLMQLGQQVQGRWQDIQRDLASRTVEATAGGGMVRVVVD
                     GRGQLRGVWIDPTVFADRDADLLSELVLSAVAQAQRRANELAQADLQRLPAAPPGP"
     gene            1176..1763
                     /gene="recR"
                     /locus_tag="VFU45_10000"
     CDS             1176..1763
                     /gene="recR"
                     /locus_tag="VFU45_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU5359443.1"
                     /translation="MAALDELVTELARLPGIGRKTAQRLAFHLLQQPKGQIERLGAAL
                     ATVAERIHPCAVCGNLTEDEPCEYCADPRRDAALLCVVEDPAGVAAVERAAGFRGRYH
                     VLGGRLSPLDGIGPEALRIDALLERVAGGVREVILATNPSMEGEATATYLQQVLAGRG
                     VRVTRLARGLPMGGDLDYVDGVTLAHALSARQEVR"
     gene            1760..2032
                     /locus_tag="VFU45_10005"
     CDS             1760..2032
                     /locus_tag="VFU45_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359444.1"
                     /translation="MRRGRAALFAGGAAAGLALGWLLARRHIGRHRADLFSLRPWRRL
                     SALAGLAGTPRIETVRLLRDYLAWEPRPLLRRRARAILRQMEATLG"
     gene            2051..2542
                     /locus_tag="VFU45_10010"
     CDS             2051..2542
                     /locus_tag="VFU45_10010"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEU5359445.1"
                     /translation="MSGASSWSFREEDFKRIQVVLADFLRESTARTGLIVDRTGQLVA
                     TVGEAPTFDAVAFASLTAADFSANDQLAKMIGEKEFGSLFHQGERESMYLADVAHRVI
                     LVVLFDNRTTLGMVKLRVKTTVGELTQVFTDMFGRDGSASKAQMETGFVGEAEDEIDK
                     LFG"
     gene            2564..3238
                     /locus_tag="VFU45_10015"
     CDS             2564..3238
                     /locus_tag="VFU45_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEU5359446.1"
                     /translation="MSMINYASREINCKLVYYGPGLGGKTTNLEHVYGKVSPNARGKM
                     ISLATETERTLFFDFLPVDLGTIRGFKTRFHLYTVPGQVYYNASRKLILKGVDGVVFV
                     ADSQIERMEANIESMQNLYDNMSQQGYDLSKVPFIIQYNKRDLPNAAPLAELQAALNP
                     GWPVEDPTRQKLTPDPNHEGEYLVFQQGGEWIERAPYYEAVAVTGDGVFDTLKAVSKL
                     VLKTLG"
     gene            3251..3901
                     /gene="pgsA"
                     /locus_tag="VFU45_10020"
     CDS             3251..3901
                     /gene="pgsA"
                     /locus_tag="VFU45_10020"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU5359447.1"
                     /translation="MWTLPNIITLVRIALTPVIALLPFIQGYWPKFIAFLVFLIAAVS
                     DVWDGWLARRRHEVTDLGKLLDPVADKLLLLATLIPIYFISQTRHDLYDIPLWGSVPL
                     WVCALLIGREFAMTAFRQWARHRGVVIPAEGVGKFKAVFQNIYIGATMIWFAYRDAAK
                     PLGWVNSPFWRFWNSFHGWFVAVTLGIALVLTITSFVTYLVRHRALFTTGEFRARR"
     gene            3927..>4437
                     /locus_tag="VFU45_10025"
     CDS             3927..>4437
                     /locus_tag="VFU45_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU5359448.1"
                     /translation="MNVELVTIGTELLLGFTVDTNSAFAGQVLADHGVRVVRRTSVPD
                     DPAAIRTAVGEALARTGTVITTGGLGPTRDDITKTVIAGMFGAPLEFQEPLWQALVER
                     YRVRGRVPVASNRSQAEVPRGATVLPNQWGSAPGLWLEGAPGLVILLPGVPVEMRNLL
                     VREVLPRLAA"
BASE COUNT          643 a         1577 c         1549 g          668 t
ORIGIN      
        1 ctggccggcg ccgccgaggc gctccgcgcc ctgctgatgc tgcagctcgg ggcggagccc
       61 gaggggatca ccgacgcgct ccgccaggcg ctcgcggggt acgccgaccg gctctccagc
      121 ggcgacctcc tccggatgct caagatcgtc gccgaccagg aggggaacgt ccggcggagc
      181 ggcaatcccc ggctcgtggt cgagaccacg ctgctccgct gggcgatgat ggaccggacc
      241 gtggacctgg aacgggtgct ggcgcagggc gggggagcac cggccgcacc ccctgccgcc
      301 cgcgcggctc cggcgcgcag cgtcccgtcg ccggcaccgg cagcgagccc gcccccggca
      361 gcggcgggcg cgggccccgc accggttccc gacacggcgg tcaccagcgc tccattctcg
      421 ctcgagggcg tccgctcggt ctgggacgag gtggtggagg cggccggtcg ggaaaagaag
      481 atgctgggga tcgccctcgc ggcgacccga cccgtcgctg ccgaccggcc ggtggtgacg
      541 ctcgagctca ccgaggagaa tccgatgcac gccgaggcgc tggagcgact gcgcgggagc
      601 gtgcaggcgc tcctcggcgc ccggctcggc tccgcggtca cgatccaggt gcggccgccg
      661 gccgggggcg gtgcgcccgc ccggcccaag cggatgacgg aggccggcaa ccgtgccgag
      721 cgcctcagca agctgcgggg caaggatccg gcgctcgatg cggccgcctc ggagttagat
      781 ttggaggtca tggagtgatc gccgttcctt gcatcctgca agtgggcggc ctaatttccg
      841 gcgtcttcga gcgggatcca tcagcagcat ggcagacttt cagcagttga tgcagctcgg
      901 ccagcaggtc cagggccggt ggcaggacat ccagcgggat ctcgcgtccc gtaccgtcga
      961 ggcgactgcg ggcggcggga tggtccgggt cgtcgtggac gggcggggac agctccgcgg
     1021 ggtgtggatc gacccgaccg tgttcgccga ccgcgacgcc gacctcctct ccgagctcgt
     1081 gctctcggct gtggcccagg cgcagcgtcg cgccaatgaa ctggcgcagg ccgacctgca
     1141 gcgcctgccc gccgctcccc ccgggccgtg acctggtggc cgccctcgat gagctggtga
     1201 ccgagctggc ccggctgccc gggatcggtc ggaagaccgc gcagcggctg gcgttccacc
     1261 tgttgcagca acccaaggga cagatcgaac ggctgggcgc ggcgctggcc actgtggccg
     1321 agcggatcca tccctgcgcc gtgtgcggca acctgaccga ggacgagccc tgcgagtact
     1381 gcgccgaccc tcggcgcgac gcggcgctcc tctgcgtggt cgaggacccg gccggagtgg
     1441 cggcggtgga gcgggcagcg ggattccgcg gccggtacca cgtcctcggc gggcggctct
     1501 cgccgctgga tgggatcggg cccgaggcgc tccgcatcga tgcgctgctc gaacgggtgg
     1561 ccggcggggt gcgggaggtg atcctggcga cgaacccctc gatggaaggg gaggccaccg
     1621 ccacctacct ccagcaggtg ctggccggtc gtggggtacg ggtgacccgc ctggcgcgcg
     1681 ggctcccgat gggcggggac ctcgattacg tggacggcgt cacgctcgcg catgcgcttt
     1741 ctgcgcggca ggaggtccgg tgaggcgcgg ccgtgcggcg ctcttcgccg gtggggcggc
     1801 ggcaggactc gccctggggt ggctcctggc ccggcgccat atcgggcgcc accgcgccga
     1861 cctcttcagc ctgcggccgt ggcggcgact ttcggccctg gccggcctgg ccgggacgcc
     1921 ccggatcgag accgtgcggc tgttgcgcga ttacctggcg tgggaacccc ggccgctcct
     1981 gcggcgccgg gcccgtgcca tccttcggca gatggaagcc accctcggct gacttgcgag
     2041 aggaatcctg atgtccggtg cgtcgagctg gtccttccgc gaggaggact tcaagcggat
     2101 ccaggtcgtg ctggccgact tcctccggga gtcgaccgcc cgcaccgggc tgatcgtgga
     2161 ccgcaccggt cagctcgtcg ccacggtggg cgaggcgccc accttcgacg cggtggcgtt
     2221 cgcgtcgctc accgccgccg acttcagcgc caacgaccag ctcgccaaga tgatcggcga
     2281 gaaggaattc gggtcgctgt tccaccaggg cgagcgggag tcgatgtacc tcgccgacgt
     2341 ggcccaccgg gtcatcctgg tggtgctgtt cgacaaccgc acgacgctgg ggatggtcaa
     2401 gttgcgggtg aagaccaccg tgggcgaact cacccaggtg ttcaccgaca tgttcggccg
     2461 tgacggcagc gccagcaagg cgcaaatgga aaccggtttc gtcggcgagg cggaagacga
     2521 gatcgacaag ctcttcggct gagcttcagg agactaccga cacatgtcga tgatcaacta
     2581 cgcttcacgc gagatcaact gcaagctcgt ctattacggg ccgggtctcg gcgggaagac
     2641 caccaacctc gagcacgtct acggcaaggt gtcgccgaac gcgcgcggga agatgatctc
     2701 cctcgcgacg gagaccgagc ggaccctctt cttcgacttc ctgcccgtcg acctcgggac
     2761 catccgcggc ttcaagaccc gcttccacct ctacaccgtc ccgggccagg tgtactacaa
     2821 cgcctcgcgc aagctgatcc tcaagggggt cgacggcgtg gtgttcgtgg ccgacagcca
     2881 gatcgagcgg atggaggcga acatcgagtc gatgcagaac ctgtacgaca acatgagcca
     2941 gcagggctac gacctgagca aggtcccgtt catcatccag tacaacaagc gcgacctccc
     3001 caacgccgcc cccctggccg agctccaggc ggcgctcaac ccgggctggc cggtcgaaga
     3061 tcccaccagg cagaagctca cccccgaccc caaccatgag ggcgagtacc tcgtcttcca
     3121 gcagggcggg gagtggatcg agcgggcgcc ctactacgag gcggtggcgg tcaccggcga
     3181 cggcgtcttc gacaccctca aggcggtcag caagctcgtc ctcaagaccc tcggctgacg
     3241 ggccccggcg atgtggacgc tgccgaacat catcaccctc gtccgcatcg ccctgacccc
     3301 ggtcatcgcg ctcctcccgt tcatccaggg ctactggccc aagttcatcg ccttcctcgt
     3361 gttcctcatc gctgccgtga gcgacgtgtg ggacggctgg ctcgcccgcc ggcgccacga
     3421 ggtgaccgat ctcggcaagt tgctcgaccc cgtggccgac aagctcctgc tcctggccac
     3481 gctcatcccg atctacttca tctcccagac ccgtcacgac ctctacgaca tcccgctgtg
     3541 gggcagcgtc cccctctggg tgtgcgccct gctcatcggg cgcgagttcg cgatgaccgc
     3601 cttccgccag tgggcccggc atcgcggcgt cgtgatcccc gccgagggcg tcggcaagtt
     3661 caaggcggtc ttccagaaca tctacatcgg cgccacgatg atctggttcg cctatcggga
     3721 tgcggccaag ccccttggct gggtcaacag ccccttctgg cgcttctgga actcgttcca
     3781 cggctggttc gtcgccgtga cgctcggcat cgcgctcgtc ctcaccatca cgtcgttcgt
     3841 cacctacctc gtccggcacc gggcgctgtt caccaccggc gaattccggg cgcgtcggta
     3901 ggacggcgcc ggggccgcac atccagatga acgtcgagct cgtcaccatc ggcaccgagc
     3961 tgctgctcgg attcacggtc gacacgaaca gcgccttcgc cggccaggtc ctcgccgacc
     4021 acggcgtgcg cgtggtgcgc cgcacgtcgg tgcccgacga ccccgccgcg atccgtactg
     4081 ccgtcggcga ggcgctcgcg cgcaccggca ccgtgatcac caccggcggc ctcggcccca
     4141 cccgcgacga catcaccaag accgtcatcg ccgggatgtt cggcgcgccg ctggagttcc
     4201 aggagccgct ctggcaggca ctggtggaac gctaccgcgt ccgtggccgg gtgccggtgg
     4261 cgagcaaccg gagccaggcc gaggtgcccc gcggcgccac ggtgctgccg aaccagtggg
     4321 ggagcgcgcc ggggctctgg ctggagggag cgccggggct cgtcatcctc ctgcccggcg
     4381 tgccggtgga gatgcggaac ctcctggtcc gcgaggtcct gccgaggctg gcggcgc
//
LOCUS       DASUAP010000373         7639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_326839, whole genome shotgun sequence.
ACCESSION   DASUAP010000373 DASUAP010000000
VERSION     DASUAP010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326839"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1765
                     /locus_tag="VFU45_10030"
     CDS             71..1765
                     /locus_tag="VFU45_10030"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5359449.1"
                     /translation="MRRFLVLAALGLGSSLPLAAQQQPPVFTHADTLRGSIGPGRAWW
                     DVVFYDLHVTVDPADSSIDGWNGITYRVTGPAREMQLDLQPPLELDSVKQGRRRLSVR
                     EDGSAWFVTLEAKQPLGAVRTVTAFYHGKPRVAKRPPWDGGFSWQLDSLGNHWVATTS
                     EGIGASIWWPNKDTQADEPDSQRIAITVPDPMIDVSNGRLRSTVHHPDHTTTYEWFVA
                     DPINNYDVAVNAGSYAHWQDTIQGEAGPLTLDFWPLAYHLDTAKVQFQQARTMLACFE
                     HWFGPYPWYKDGYKLVETSHLGMEHQSGIAYGNHYKNGYLGRDLSHTGVGLGWDFIIV
                     HESAHEWWGNNLTDQDVADMWIHEGFANYAENLYVECQSGKAKGAEYVIGTRKNIRND
                     EPIVGIFGVNHEGSGDMYYKGGNMLHTIRQVINDDEKWRGILRGLNHDFWHQVVTGQQ
                     VRQYISDKAGIDLSRVFAQYLETTKVPVFEYRISSDTLSYHWADVVPGFDMPLRVTLA
                     DSTFSWIHPTEAWQTAPVHPGPAGFKVDENFYVIPRDLNAPTPAVKDSTREVRSKK"
     gene            2038..4341
                     /locus_tag="VFU45_10035"
     CDS             2038..4341
                     /locus_tag="VFU45_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5359450.1"
                     /translation="MPPRRTGRAGARKKGAEPDYRLIFRQAPIPAYIAEVGSTRILDV
                     NQAALERYGYTRAEFLRLSARDIRPPEDAARYDAFVAEQVPLRDAFIPAGLWRHCTRA
                     GEVFDVEVSWARVQWKGRRAYIAFVRDLSAQRSAEAALRRWGTVFRAVSEAVIISDLD
                     GALVDANPSAEALLGRPRSEMVGRPIGDWHAPGSVTNEEIRAVAARTGYWSGRLRFLR
                     PDGSVRVTDSTIVPLFDNDGRLNGYAGVNRDVTDQERVAESARSNHERLEAVITSSPL
                     AMYVLDCDLVVQAWNPAAERIFGWSAVEAIGRVLPTLGPGDIAEARALVARVEAGGTL
                     SRVELTRHRKDGSEVSISLSSAPLRDASGKLVGLSSIAEDVTEGRRMEAQLRQAQRME
                     SVGRLSGGIAHDFNNLLTAILGSAELLRATLREGSPELEEVNEIEKASRRAADLTRQL
                     LAFSRQQMLAPAVVDPNALVIDLKRMLGRVVGEDIHFEARLRPDIGKVRADPGQLGQV
                     IMNLVVNARDAMPEGGMLTIATTLAELDERYVERHPGGATGEFIVISVTDTGTGIPAG
                     VLPHIFEPFYTTKAPGVGTGLGLSTAYGIVKQSGGYIWAYSEVGKGTTFKVYLPRWTG
                     GGEVDKKPRHTPVPTNLRGTETILAVEDEPGIRRLVARTLSEHGYKVIAASDALEAVT
                     KLAQLEGELDLLATDVVMPGMGGRELAEHVRSTHPGTKVLFLSGYTEDAIVRHGIHTP
                     TQAFLEKPFTPAELLRKVRGVLDQQGK"
     gene            4428..5651
                     /locus_tag="VFU45_10040"
     CDS             4428..5651
                     /locus_tag="VFU45_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU5359451.1"
                     /translation="MPSPVSAYAITAPGIEAITARELTALGLPVTATEPGGVAFSATP
                     VQLYAANLHSRTASRIIVRVVEFSARTFFELERHAKKVPWERFAAPGSAVSFRVTSRK
                     SKLYHQGAIEERLARWAGERGFACRSEESRVKSEKAEVEIGFSDPAAGTPEDRESPFH
                     PSLFTLHSQLFLIRLHRDKVTVSADSSGELLHRRGYRLATAKAPVRETLAAAMLLASG
                     WDGSAALIDPLCGAGTIPIEGALIARNIAPGARRSFAFERWPEFDAAAWAKLREAALN
                     GERATVNGTIEGADRDAGAIAASVANAERAGVANDITFVQRALDAWADVPSHRGGWLV
                     TNPPYGVRVGEDAQLAALYATLGAVARQQVPGGQIGVLSADPRLDARIGLPLTERFAA
                     ENGGIGVRFVTGVVS"
     gene            5806..6294
                     /locus_tag="VFU45_10045"
     CDS             5806..6294
                     /locus_tag="VFU45_10045"
                     /inference="COORDINATES: protein motif:HMM:NF016130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEU5359452.1"
                     /translation="MLNRREWLTRVGLGAVGLTVLPRLLEGSTPVRAGGVVAPIGIKV
                     YKSASCGCCAAWVTYMNANGFKCDVSDLDDSALDAKKAQLGVTDAVRSCHTAVVGKYL
                     IEGHVPADVVKRLLREHPAALGLAVPGMVPGSPGMDGGTAQHYDVVLFQRGGASRTYV
                     TR"
     gene            6294..6947
                     /locus_tag="VFU45_10050"
     CDS             6294..6947
                     /locus_tag="VFU45_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5359453.1"
                     /translation="MTAGLSLVAPAPHGGRPVLQGGAPVGSARGALILVHGRGADAES
                     MLELAQTVSPDDLAWFAPEAARNTWYPFSFLEPMERNEPALGSALAAVHGLVEALGVA
                     GFPPERIALLGFSQGACLSLEYAARRARRFGAVIGLSGGLIGPPGTLRSYPGSFAGTP
                     VFLGCSDVDPHIPVERVHESEEVFRRMGAEVDARIYPGMGHTVNADEVKAVRAILAR"
     gene            7142..>7639
                     /locus_tag="VFU45_10055"
     CDS             7142..>7639
                     /locus_tag="VFU45_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5359454.1"
                     /translation="MPLPSRTLGRSGLTVSAQGLGCMGMSEFYAGRDDVESVATIHRA
                     LDLGVTFLDTADVYGPYLNEELVGRAIKARRNEVTLATKFGFVRSTDPGVRAVNGRPE
                     YVMQACDASLRRLQTDHIDLYYQHRVDPDVPIEETVGAMSRLVQQGKVRFLGLSEAGL
                     DTIRRA"
BASE COUNT         1230 a         2601 c         2588 g         1220 t
ORIGIN      
        1 gcgccgcccc tgctgaatgc gccgacgcgt gataccttcc ccattccttt cattcatcga
       61 gccgccactc atgcgtcgtt tcctggtcct cgccgccctc ggcctcgggt cctcgctccc
      121 cctcgccgcc cagcagcagc ccccggtctt tacccacgcc gacacgctgc gcgggtcgat
      181 cgggccgggg cgcgcgtggt gggacgtcgt cttctacgac ctccacgtca cggtggaccc
      241 ggccgacagc agcatcgacg gctggaacgg gatcacctac cgggtcaccg gtcccgcgcg
      301 cgagatgcag ctcgacctcc agccgccgct cgagctcgac agcgtgaagc agggccgccg
      361 gcgcctgagc gtgcgggagg acgggagcgc ctggttcgtg accctcgagg cgaagcagcc
      421 cctcggcgcc gtccgcaccg tgaccgcgtt ctaccacggc aagccgcggg tggccaagcg
      481 tccgccgtgg gacggcggct tcagctggca gctcgacagc ctcggcaatc actgggtggc
      541 cacgacgtcg gaagggatcg gtgccagcat ctggtggccc aacaaggaca cccaggccga
      601 cgaacccgac agccagcgca tcgccatcac ggtgcccgat ccgatgatcg acgtctccaa
      661 cggccggctc cggagcaccg tgcaccatcc cgaccacacc accacgtacg aatggttcgt
      721 ggccgacccg atcaacaact atgacgtggc ggtgaacgcg gggagctacg cccactggca
      781 ggacaccatc cagggggaag cgggcccgct gaccctcgac ttctggccgc tggcctacca
      841 cctcgacacc gcgaaagtcc agttccagca ggccaggacg atgctcgcct gcttcgagca
      901 ctggttcggg ccgtacccgt ggtacaagga cggctacaag ctggtggaga cctcgcacct
      961 ggggatggag caccagagcg gcatcgccta cggcaaccac tacaagaacg gctacctggg
     1021 gcgcgacctc tcccacaccg gcgtggggct cggctgggac ttcatcatcg tgcacgagag
     1081 cgcgcacgag tggtggggca acaacctgac cgaccaggac gtggccgaca tgtggatcca
     1141 cgagggcttc gccaactatg cggagaacct ctatgtggag tgccagtcgg ggaaggcgaa
     1201 gggcgcggag tacgtgatcg gcacccggaa gaacatccgg aacgacgagc ccatcgtcgg
     1261 gatcttcggc gtcaaccacg aaggttcggg cgacatgtac tacaagggcg ggaacatgct
     1321 gcacaccatc cgccaggtca tcaacgacga cgagaagtgg cgcggcatcc tgcggggcct
     1381 caaccacgac ttctggcacc aggtggtgac agggcagcag gtccggcagt acatcagcga
     1441 caaggcgggg atcgacctga gccgggtgtt cgcccagtac ctggagacca cgaaggtgcc
     1501 ggtcttcgaa tacaggatca gcagcgacac actgtcgtat cactgggccg acgtcgtgcc
     1561 cggtttcgac atgccgctcc gggtgacgct cgcggattcg acattctcgt ggatccaccc
     1621 gaccgaggcg tggcagacag cgccggtgca cccgggcccg gcgggattca aggtcgacga
     1681 gaacttctac gtgatcccga gggacttgaa cgcccccact ccggcggtga aggactcgac
     1741 gagggaagtg agaagtaaga agtgagaagt gagaagggga agtaagaagg ggaagtaaga
     1801 agtcagaagt gagaggtgag acgcgagagt gaaaagggaa gaggggcgca ccgctctccc
     1861 ttccctctcc tccctcccct ctcgcctctc ccttccccct tcccccttct tacttcttac
     1921 ttctcccttc ttacttctta cttctcactt cttacttccc cctctcccgc tggcgtcagg
     1981 gtggcccggc ggtaccttcc ggaagtggtc ggaatgtcgc cattcacggg ggacgggatg
     2041 ccgcctcgtc gcactggaag ggccggcgct cgaaagaagg gggcggagcc tgactatcgc
     2101 ctgatcttcc ggcaggcgcc catccctgcg tacatcgccg aggtcgggtc gacccgcatc
     2161 ctggacgtga accaggctgc gctcgagcgg tatgggtata cccgcgccga attcctccgc
     2221 ctgagcgcgc gggacatccg cccgccggag gacgcggcgc gctacgacgc gttcgtcgcc
     2281 gagcaggtgc cccttcgcga tgcgttcatt cccgccggcc tctggcgtca ctgcacccgc
     2341 gccggcgagg tgttcgacgt cgaggtgtcg tgggcgcggg tccagtggaa gggtcggcgg
     2401 gcgtacatcg ccttcgtgcg cgacctctcc gcgcagcgct cggcggaggc ggcgctccgg
     2461 cgctggggca cggtgttccg tgcggtgagc gaggcagtga tcatcagcga cctggacggg
     2521 gcgctggtgg acgcgaaccc gtcggccgag gcgctcctgg gccggccccg ctcggagatg
     2581 gtggggcgcc cgatcggcga ttggcacgcg cccggctcgg tcaccaacga ggagatccgc
     2641 gcagtcgcgg cacgtacggg atattggagt ggacggctcc ggttcctgcg cccggacggg
     2701 tcggtgcggg tcaccgattc gaccatcgtt ccgctgttcg acaatgacgg gcggctgaac
     2761 ggttatgccg gggtcaaccg cgacgtcacc gaccaggagc gggtggccga gtcggcgcgg
     2821 tcgaaccacg agcggctgga ggcggtcatc acctcgtcgc cgctcgcgat gtacgtgctc
     2881 gactgcgacc tcgtggtgca agcgtggaac ccggcggccg agcggatctt cggttggtcg
     2941 gcggtggagg cgatagggcg ggtgctcccg accctcggcc ccggggacat cgccgaggcc
     3001 cgggcgctgg tggcgcgcgt ggaggccggc gggacgctct cgcgtgtgga gctcacgcgc
     3061 catcggaagg acgggtcgga ggtgtcgatc tcgctgtcgt cggcgccgct ccgggatgca
     3121 tcggggaagc tcgtcggcct gtcgtcgatc gccgaggacg tcaccgaggg gaggcggatg
     3181 gaggcacaac tcaggcaggc ccagcggatg gagtcggtgg gccggctgtc gggagggatc
     3241 gcgcacgact tcaacaacct cctgaccgcc atcctcggat ccgccgagct cctccgcgcg
     3301 acccttcggg agggctcgcc cgagctcgag gaggtgaacg agatcgagaa ggcctcccgc
     3361 cgcgcggccg acctgacccg ccagttgctc gcgttcagcc gccagcagat gctggcgccc
     3421 gccgtcgtgg atcccaacgc cctcgtcatc gatctcaagc ggatgctggg ccgcgtcgtc
     3481 ggcgaggaca tccacttcga ggcgcgcctc cggcccgaca tcgggaaggt ccgcgcggat
     3541 cccggccagc tgggccaggt gatcatgaac ctggtggtga acgcccggga cgcgatgccc
     3601 gagggcggga tgctcaccat cgcgacgacg ctcgccgagc tcgacgagcg ttacgtcgaa
     3661 cgccaccccg gcggcgccac tggtgagttc atcgtcatca gcgtcaccga cacgggcacg
     3721 gggatccccg ccggggtcct gccgcacatc ttcgagccgt tctacaccac caaggcgccg
     3781 ggcgtgggga cggggctcgg gctctcgacc gcctacggca tcgtgaagca gagcggcggc
     3841 tacatctggg cgtacagcga agtggggaag gggaccacct tcaaggtgta cctcccgcgc
     3901 tggaccggcg gcggcgaggt cgacaagaag ccgcgccaca ccccggtgcc caccaacttg
     3961 cgcggcaccg agacgatcct tgcggtggag gacgagccgg ggatccggcg cctcgtggcc
     4021 cgcacgctct ccgagcacgg ctacaaggtc atcgcggctt cggacgcgct ggaggcggtg
     4081 accaagctcg cccagctgga gggggaactc gacctcctgg ccaccgacgt ggtaatgccc
     4141 gggatgggcg ggcgcgagct ggccgaacac gtccgctcca cccatccggg caccaaggtc
     4201 ctcttcctct cgggctacac cgaagacgcg atcgtccgcc acggcatcca caccccgacc
     4261 caggcgttcc tcgaaaagcc gttcaccccc gccgaactcc tccgaaaggt gcggggggtg
     4321 ctggaccagc aggggaagtg aaaagtgaag agtgaagagg gtgcgtgaag tactcctttt
     4381 cactcttcac tcttcactct tcactccctt ccccgtaccc ttcccgcgtg ccctcccccg
     4441 tctccgccta cgccatcacc gcccccggca tcgaggcgat caccgcccgg gaactgacgg
     4501 cgctcggtct cccggtgacc gccacggagc ccggcggcgt cgcgttctcc gccacaccgg
     4561 tccagctcta cgccgccaac ctccactccc gcacggcgag ccggatcatt gtgcgcgtgg
     4621 tggagttctc ggcccgcacc ttcttcgagc tggagcgcca cgcgaagaag gtgccgtggg
     4681 agcgctttgc cgcgccgggg agtgccgtgt cattccgggt cacgagccgg aagtcgaagc
     4741 tctaccatca gggagccatc gaggaacggc tggcacgatg ggcgggggag cgggggttcg
     4801 cctgccggag tgaagagtca agagtcaaga gtgaaaaggc cgaagtggag attggatttt
     4861 cggatcccgc ggctggaacg ccggaggacc gagagtcccc ttttcaccct tcactcttca
     4921 ctcttcactc ccagctcttc ctcatccgcc tccaccgcga caaggtgacc gtgagcgcgg
     4981 acagttcggg cgagctcctc catcggcggg gataccggct ggccaccgcc aaggcaccgg
     5041 tgcgggagac gctcgcggcg gcgatgctcc tcgcgagcgg gtgggacggt tccgctgcgc
     5101 tcatcgaccc gctctgcggc gccgggacga tcccgatcga gggggcgctc atcgcccgga
     5161 acatcgcgcc gggtgcacgg cggagcttcg ccttcgagcg gtggccggaa ttcgatgcgg
     5221 cagcgtgggc gaagttgcgt gaagcggcgt tgaacggtga acgggcaacg gtcaacggga
     5281 cgatcgaggg cgccgaccgg gatgccggcg ccatcgccgc gtcggtggcc aacgcggagc
     5341 gcgccggtgt ggcgaacgac atcaccttcg tccagcgggc cctcgacgcc tgggccgacg
     5401 taccgtcgca ccgaggcggc tggctggtga cgaatcctcc ctatggcgtg cgcgtaggag
     5461 aagacgcaca attggccgcc ctctacgcca cgctcggcgc cgtggcccgg cagcaggtgc
     5521 ccggagggca gatcggcgtc ctttcggcgg acccccgcct cgacgccagg atcgggctcc
     5581 cgctcaccga gcgcttcgca gccgagaacg gcgggatcgg cgtgcggttc gtgacggggg
     5641 tggtatcttg atgggagcta tcagctatcg gctatcagcc gctccgtgcg cgatcgaagc
     5701 ttgttgaaac cattcaagta tcccggcgtc cgttcgctga tagctgatag ctgatagctg
     5761 atagctgata gctgatagct gatagctcac accgaggaac gcaccatgct caatcgcaga
     5821 gaatggctca cccgggtcgg gcttggggcc gtgggactca cggtgcttcc acgcctgctc
     5881 gagggatcga cgccggttcg ggccggcggg gtcgtggcgc cgatcggcat caaggtctac
     5941 aagtcggcga gttgcggttg ctgcgccgcc tgggtgacct acatgaacgc caacggcttc
     6001 aagtgcgacg tgtccgacct cgacgactcc gcgctcgatg ccaagaaggc ccagctcggc
     6061 gtgaccgacg cggtccggtc ctgccacacc gcagtggtcg ggaagtacct gatcgagggg
     6121 cacgtccccg ccgacgtggt caagcgtctc ctcagggagc acccggcggc cctgggcctt
     6181 gccgtgccgg ggatggtgcc cggctccccg gggatggatg gcgggacggc gcagcactac
     6241 gacgtcgtgc tcttccagcg gggtggcgcg tcgcggacgt acgtcacccg ctgatgaccg
     6301 ccggtctgtc gctcgtggcg cccgccccgc acggcgggcg cccggtgctt caggggggtg
     6361 cgccggtagg ctcggcgcgc ggcgcgctga tcctggtcca cgggcgcggc gccgacgcgg
     6421 agtcgatgct ggaactggcg cagaccgtgt cgcccgatga cctcgcctgg ttcgcgcccg
     6481 aggcggcccg caacacctgg tatccgttca gcttcctcga gccgatggag cggaacgagc
     6541 cggcgctggg ctccgccctc gccgcggtgc acggccttgt ggaagcgctc ggcgtcgctg
     6601 gctttccgcc cgagcggatc gccctgctcg gattttcgca gggagcgtgc ctctcgctcg
     6661 agtatgccgc gcgccgcgcc cggcgcttcg gcgcggtgat cggactctcc ggcgggctca
     6721 tcgggccccc tggcacgctc cgcagctatc cggggtcctt tgccggcacc ccggtcttcc
     6781 tcggctgcag cgacgtcgat ccccacatcc cggtcgaacg cgtgcacgaa tcggaggaag
     6841 tcttccggcg gatgggtgcc gaggtcgacg cccggatcta cccggggatg ggacacaccg
     6901 tgaacgcgga tgaggtgaag gcggtgcgag cgatccttgc gaggtgagcg cggcagttag
     6961 cgcttagagc ctagagccta gagcccagag cctagggctt agcgcttagg gcatagggct
     7021 tatggcttag ggctcatgcg gcgtacatct tcccgctcac gtcttacgtc tcacgtctca
     7081 cgtctcccct ctcacgtctc acctcttacc tcttacctct cacctctcac ctcttacctc
     7141 catgcccctc ccttctcgca cactcggtcg ctccggcctc accgtctccg cccagggtct
     7201 cggctgcatg gggatgtcgg agttctatgc cggtcgggac gatgtcgagt cggtcgccac
     7261 gatccaccgc gcgctcgatc tcggcgtcac cttcctcgac accgccgatg tctacggccc
     7321 ctacctcaac gaggagctgg tggggcgcgc gatcaaggcg cggcgcaacg aggtgacgct
     7381 cgccaccaag ttcgggttcg tgcgcagcac cgatcccggc gtgcgcgccg tgaacggccg
     7441 ccccgagtac gtgatgcagg cgtgcgatgc gtccctcaga cggctgcaga ccgaccacat
     7501 cgacctttac tatcagcacc gcgtggaccc cgacgttccg atcgaggaga cggtcggggc
     7561 gatgtcccgc ctggtccagc aggggaaggt gcgcttcctc ggcctctcgg aggcgggact
     7621 cgacacgatc cgccgggcg
//
LOCUS       DASUAP010000374         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_817193, whole genome shotgun sequence.
ACCESSION   DASUAP010000374 DASUAP010000000
VERSION     DASUAP010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817193"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFU45_10060"
     CDS             <1..309
                     /locus_tag="VFU45_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEU5359455.1"
                     /translation="VVRTIPQAEGVAAILAARGRTMDRVLFFDVSDDEILARLERRRG
                     LEHRADDDPAAVATRLRAYREQTAPVLAWYRARGPVDVIPAVGTVEEVAGRVRTLLGC
                     "
     gene            314..1060
                     /gene="map"
                     /locus_tag="VFU45_10065"
     CDS             314..1060
                     /gene="map"
                     /locus_tag="VFU45_10065"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU5359456.1"
                     /translation="MITLKSPREVETMARAGRIVHDTLELVARSVRPGISTAELDDIA
                     EAFIRSHPGATPSFKGLYGFPRTLCTSINEEIVHGIPSKKRVLVEGNILSVDVGVKLD
                     GLHADAATTVPVGEISPDAAKLLQVTQECLRAGIAQAVAGNHVGDIGHAVQTVAESHG
                     YGVVRELVGHGIGRAFHEEPQVPNHGRPKRGPRLAAGMTIAIEPMITMGDFATRTLDD
                     KWTVVTEDGSLSAHFEHTVAITDQGPRILT"
     gene            complement(1065..>3143)
                     /locus_tag="VFU45_10070"
     CDS             complement(1065..>3143)
                     /locus_tag="VFU45_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEU5359457.1"
                     /translation="LESFRDSVATVSDTAGLRRFERSLGRPPATSPIDALRDAIVALR
                     LGTRGDLTRALDSCRAVARAEPDWGYPWFCIGLAAGARGRSTAAEPLNLGLIPGRGDF
                     ERSADAFLAAARAEPGFVPAAAALGELVLGREGAERRTEALPIIRAAAPTALRTPPSL
                     LLLRGRLERLAGDPDSALAAFRAYDAGGGDHALALLEEARTELTRPVPPSDAESLYYT
                     GAALDDSVAVAGYRHDLRYLVADSALRGFDAARGEDRASWLRRFWEARDLVDLQPAGA
                     RLAAHYRRLVYAEQHYTRRNGRRIGWCDPGRGYESGLPDLDDRGVVYVRYGPPTLRVT
                     TPVWGMMPAESWRYDLPDADTVVVHFLDLHNVNDYRLAPTALSLAAVPGCGDPDAAPL
                     AVMQSRAVLLPDYDRWLSGSDLERARLGRRDIQRGEVTIPRITSTDIDPLRFARELRG
                     VGRVSVLGAADSGALAHFAFGVFVPESLAERVGRQPAVRLVAMQRGGRVDAWRGTPGD
                     SRLVAGDSGWWLVGHIALPAHTGVTYLRLAVLAGDSVGAVVGWDSVAVPNAHPGPGLS
                     PLTVGTRSRGIPWAADAGDTAFFSPFRSVRRGDPLEIYAELTGARAGASYEVALVLVP
                     TGRGLTRRLLGRAPPRISVQWRAVADGTTVPIRRTVGVGALVPGAYVIRLEVRMDGRN
                     FRAEDRVTIE"
BASE COUNT          464 a         1142 c         1160 g          377 t
ORIGIN      
        1 gtcgtgcgga ccatccccca ggcggagggc gtcgcggcca tcctcgcggc gcggggccgg
       61 acgatggacc gggtgctctt cttcgacgtg tctgacgacg agatcctcgc gcgcctggag
      121 cgccggcgtg ggctcgagca ccgggccgac gacgacccgg ccgcggtcgc gacgcgcctc
      181 cgggcctacc gggagcagac ggccccggtc ctcgcctggt accgggcccg cggcccggtc
      241 gacgtcatcc ccgcggtcgg tacggtcgaa gaagtggccg gccgggtgcg gaccctcctt
      301 gggtgctgac gccatgatca ccctcaaatc cccccgcgag gtggagacga tggcccgggc
      361 cgggcgcatc gtccatgaca ccctcgagct ggtggcgcgg agcgtccgcc ccggcatctc
      421 gacggcggag ctcgacgaca tcgccgaggc attcatccgg agccatccgg gtgccacgcc
      481 gtcgttcaag ggcctctacg ggttcccgcg caccctctgc acctcgatca acgaggagat
      541 cgtccacggc atcccgtcga agaagcgggt gctggtcgag gggaacatcc tgagcgtcga
      601 cgtgggggtg aagctcgacg gcctccacgc cgacgccgcg accacggtgc cggtggggga
      661 gatctccccg gacgcggcaa agctcctcca ggtgacgcag gagtgcctgc gggcggggat
      721 cgcccaggcg gtggcgggga accacgtggg cgacatcggc cacgcggtgc agacggtcgc
      781 cgaatcgcac ggctatggcg tggtgcgcga gctggtggga cacggcatcg gccgggcgtt
      841 ccacgaggag ccccaggtgc cgaaccacgg gcgcccgaag cgcgggcccc gcctcgccgc
      901 ggggatgacg atcgccatcg agccgatgat cacgatgggc gactttgcca cccgcacgct
      961 ggacgacaag tggaccgtcg tgaccgagga cggcagcctc tcggcgcatt tcgagcacac
     1021 ggtggccatc accgaccagg gaccccggat ccttacctga gcggtcactc gatggtgacc
     1081 cggtcctccg ccctgaagtt ccgcccgtcc atccgcactt ccaaccggat cacataagcg
     1141 cccggcacca gcgcgcccac acccacggtg cgccggatcg ggaccgtcgt gccatcagcg
     1201 accgcacgcc actgtaccga gatccggggc ggggcgcggc cgaggagccg gcgcgtgagc
     1261 ccccggcccg tcgggaccag caccagcgcc acctcgtagg aggcgccggc gcgcgcaccg
     1321 gtcagttcgg cgtagatctc gagcgggtca ccccggcgca cggaccggaa cggcgagaag
     1381 aacgccgtgt cgccggcgtc ggcagcccag gggatcccgc gcgaccgtgt cccgacagtg
     1441 agggggctga gaccgggccc ggggtgcgcg ttcggcaccg ccacgctgtc ccagccgacg
     1501 accgcgccca cgctgtcacc ggcaaggacg gcgaggcgga ggtacgtcac gccggtgtgc
     1561 gccgggagcg cgatgtgtcc gacgagccac caccccgagt cgcccgccac caggcggctg
     1621 tcacccggcg tcccccgcca ggcatccacg cggccgcccc gctgcatcgc cacgaggcgc
     1681 acggcgggct gcctgcccac ccgctccgcg aggctctccg ggacgaagac gccgaacgcg
     1741 aagtgcgcga gcgcgcccga atcggcggct ccgagcacgc tgacccggcc gaccccgcgc
     1801 aactcccggg cgaaccggag ggggtcgatg tcggtcgagg tgatgcgtgg gatggtcact
     1861 tcgccccgct ggatgtcccg gcgccccagc cgggcccgct cgaggtcgga gcccgagagc
     1921 cagcggtcat agtccggcag cagcacggca cgggactgca tcaccgccag cggtgcagcg
     1981 tcggggtccc cgcatcccgg gacggccgcc agcgacagcg ccgtgggtgc gagccggtag
     2041 tcgttcacgt tgtggaggtc gaggaaatgg acgaccacgg tgtcggcatc cggaaggtcg
     2101 tagcgccacg attccgccgg catcatcccc cagaccggcg tcgtcacccg gagtgtcggc
     2161 gggccgtacc gcacgtagac gaccccgcga tcatcgaggt cgggaaggcc ggactcgtac
     2221 ccgcggcccg ggtcgcacca gccgatgcgg cggccgttgc ggcgggtgta atgctgctcg
     2281 gcgtagacca ggcgccggta gtgcgcggcg aggcgcgcac cggccggctg caggtccacc
     2341 aggtcgcgag cttcccagaa gcggcggagc cacgatgccc ggtcctcccc tcgggcggca
     2401 tcgaacccgc gcaatgccga gtccgccacc aggtagcgga gatcgtggcg gtatcccgcc
     2461 accgccacgc tgtcatcaag tgcggccccg gtgtaataca gggattcggc gtctgacggg
     2521 ggaacgggcc gggtcagctc ggtgcgcgcc tcctccagca gcgcgagggc gtggtcacca
     2581 ccgccggcgt cgtacgcgcg gaaggcggcg agggcggagt cggggtcgcc tgccagccgc
     2641 tccaggcggc cgcggaggag caacagcgat ggcggggtac gcagcgcagt cggcgctgcc
     2701 gcccggatga tggggagtgc ctccgtcctc cgctccgctc cctcccgccc gagcaccagc
     2761 tctccgagcg ccgcggccgc cggtacgaag ccgggctccg ctcgcgcggc ggccaggaag
     2821 gcgtcggcgc tccgttcgaa gtcgccccgg ccggggatga ggccgaggtt gagcggctcg
     2881 gcggcggtgc tcctcccgcg agccccggcc gccagcccga tgcagaacca agggtagccc
     2941 cagtccggtt cggcccgcgc gacggcccgg cagctgtcca gggcgcgggt gaggtcgccc
     3001 cgcgtcccga gtcgcagcgc gacgatcgcg tcgcggaggg cgtcgatcgg tgaggtggcg
     3061 gggggccggc cgagcgatcg ctcgaagcgg cggagtccgg cggtgtcgga cacggtcgcg
     3121 accgaatcgc ggaatgactc aag
//
LOCUS       DASUAP010000375         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_776746, whole genome shotgun sequence.
ACCESSION   DASUAP010000375 DASUAP010000000
VERSION     DASUAP010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776746"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..217)
                     /locus_tag="VFU45_10075"
     tRNA            complement(144..217)
                     /locus_tag="VFU45_10075"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(181..183),aa:Pro,seq:tgg)
     gene            complement(264..878)
                     /locus_tag="VFU45_10080"
     CDS             complement(264..878)
                     /locus_tag="VFU45_10080"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEU5359458.1"
                     /translation="MRLLVATRSAGKQAEARRLLEAAGHEVVFPDDAGIPESASEEAL
                     EGDDTFVGNARRKAEYFQRRTGLPTVADDSGLEVLSLGGAPGVRSKRWAEAAGTPGEV
                     DAANNAELLRRLRGAPAGKRSARYRCVLVLLRSANAVPEVFEGECAGRILEAPEGSGG
                     FGYDPLFWSPELGKAFGDATPEEKDRVSHRGRAFAKLAAFLRTL"
     gene            complement(908..1618)
                     /gene="rph"
                     /locus_tag="VFU45_10085"
     CDS             complement(908..1618)
                     /gene="rph"
                     /locus_tag="VFU45_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU5359459.1"
                     /translation="MPRPDGRPADQLRPLSLERGANPYAEGSCLIRMGGTLVHCTASV
                     EAGVPPFKKGSGEGWVTAEYAMLPRATTERTDRERRGAGGRTMEIQRLIGRSLRAAMR
                     TMMFGEYTIKVDCDVLQADGGTRTASITGGCVALADACRWVSERNGGIDPFGELVAAI
                     SVGVVESEPRLDLMYLEDRDAGVDSNVVMVHPDRFVEVQGTGEHATFDRAELDRLLDL
                     AAGGIRQLFEAQRKALGR"
     gene            complement(1605..2078)
                     /gene="rfaE2"
                     /locus_tag="VFU45_10090"
     CDS             complement(1605..2078)
                     /gene="rfaE2"
                     /locus_tag="VFU45_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEU5359460.1"
                     /translation="MTAAKIRTRAEAAAWRRGQRGPVVFTNGVFDLVHPGHVELLERA
                     RAEGAALVVGVNSDASVRRLAKGAGRPIVPEAARARVLAAFAAVDCVVLFDEDTPFEL
                     IQALEPDVLVKGADYTRDRIVGADLVEGRGGRVIRVPVVAGFSTSSIVERLRASS"
     gene            complement(2075..>3112)
                     /locus_tag="VFU45_10095"
     CDS             complement(2075..>3112)
                     /locus_tag="VFU45_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5359461.1"
                     /translation="SFVHLLAEVGVVMLLFEIGLETDLKEMFRVGTAAASVAVAGVLV
                     PFGLGYLYWAHGPHELVASTVPLAVAAVFVGATLTATSVGITARVLADLRRLDTEEAK
                     VILGAAVIDDVLGLVILSVVSTIAGGTAVGVGAGFRVLLVAIGFLVVAVVLGLKLAPR
                     LFDRIEAMRVRHVLVVFAIAFALLMAALADAAGSAMIIGAFAAGLILSGTNQFHVIED
                     RVRPVAAVLTPVFFVSVGAQANLDLLDVTHPGALAMLGVAVVLTALAFLGKLVSGWAA
                     PWGRFDRMTVGVGMVPRGEVGLIFADIGRRTGILGEPVFNAVLLMVLATTIAAPIGLK
                     KRFARLGARTA"
BASE COUNT          501 a         1107 c         1126 g          378 t
ORIGIN      
        1 gcatcgagca ccagagcgag ggcgagggcg ccggcgagcg gcgccttcaa gagcacggac
       61 cagcgggtca gcatggggtt ccaggctcgg ggtgcgggcg aaccttcggt ggcgagtgcc
      121 acgcgccact gcagtacgtc ccatcggggc ggccggattc gaaccgacga cttcctgctc
      181 ccaaagcagg cgctctaccg ggctgagcta cgccccgcga ctgcggtgaa cggtaaacgg
      241 tgaacggtga acgggaaggg tccctacagg gtgcgcagga atgccgccaa cttggcgaac
      301 gcccgcccgc ggtggctgac ccggtccttc tcctccggcg tggcgtcgcc gaacgccttg
      361 ccgagctccg ggctccagaa gagcggatcg taaccgaacc cgcccgatcc ctccggggcc
      421 tcgaggatcc ggccagcgca ctccccttcg aagacctcgg gcacggcgtt ggccgagcgg
      481 aggagcacca gcacgcagcg gtagcgggcg gaacgcttcc cggccggggc gccgcggagc
      541 cggcggagga gctcggcatt gttggcggcg tcgacttcgc caggcgttcc cgccgcctcg
      601 gcccaccgct tcgaccggac gccgggggcg ccgcccaggg agagcacctc gagcccggaa
      661 tcgtcggcga cggtcgggag cccggtccgt cgctgaaaat attccgcctt ccgccgtgca
      721 tttccgacga aggtgtcgtc gccctcgagc gcttcctcgg aggcggattc ggggatgccg
      781 gcatcgtccg ggaagacgac ctcgtggccg gcggcctcga ggaggcgccg ggcctcggcc
      841 tgcttgccgg cgctgcgggt ggcgacgagg aggcgcacgg aggggtgggc ggacgggcgg
      901 atggccgtca gcggccgagg gccttgcgct gggcctcgaa gagctgacgg ataccgccgg
      961 cagcgaggtc gagcagccgg tcgagttcgg cacgatcgaa ggtggcgtgc tcgccggtcc
     1021 cctggacctc gacgaagcga tcgggatgga ccatgacgac gttcgaatcg acgccggcgt
     1081 cccgatcctc gaggtacatc aggtcgaggc gcggctcgga ttccaccacc ccgacggaga
     1141 tggccgccac cagttcaccg aacgggtcga tcccaccgtt ccgctccgag acccagcggc
     1201 aggcgtccgc cagtgcgaca cagccgccgg tgatgctggc ggtgcgggtg ccgccgtcgg
     1261 cctggaggac gtcgcaatcg accttgatgg tgtattcccc gaacatcatt gtccgcatgg
     1321 cggcgcggag ggagcggccg atgaggcgct ggatctccat cgtccggccg ccggccccgc
     1381 gccgctcgcg atcggtgcgc tccgtcgtgg cgcgcgggag catcgcgtac tcggcggtga
     1441 cccacccttc cccgctcccc ttcttgaacg gtggcacccc ggcctcgacg ctcgcggtgc
     1501 agtgcaccag cgtgcctccc atccggatga ggcaggagcc ttcggcgtag gggttcgcgc
     1561 cgcgctcgag ggacaggggg cggagctggt cggccggacg gccgtcagga cgaggcacgc
     1621 agacgctcga cgatggagga agtggagaac ccggccacga ccgggacgcg gatgacccgg
     1681 ccgccgcgac cttcgaccag gtcggcgccg acgatgcggt cgcgggtgta atcggcccct
     1741 ttgaccagca cgtcgggctc gagggcctgg atcagctcga acggggtgtc ctcgtcgaac
     1801 aggacgacgc aatcgacggc ggcgaaggcg gcgaggaccc gggcgcgcgc cgcctccggc
     1861 acgatgggac gcccggcccc cttggcgagg cggcgcacgg acgcgtccga attgacgccc
     1921 accaccagcg ccgcgccctc ggcgcgcgcg cgctcgagga gttccacatg gcccggatgg
     1981 accaggtcga acacgccgtt ggtgaacacc accggcccgc gctggccccg gcgccaggcc
     2041 gcggcctcgg cgcgggtgcg gatcttggcc gccgtcacgc cgtccgcgcc ccgaggcgcg
     2101 cgaaacgctt cttgagcccg atcggcgccg cgatggtggt ggcgagcacc attaggagca
     2161 cggcgttgaa gaccggctcg cccaggatcc cggtgcggcg gccgatgtcc gcgaagatga
     2221 gcccgacctc gccacgcggc accatcccga cccccacggt catccgatcg aaccggcccc
     2281 agggcgccgc ccatcccgac accagcttcc cgaggaacgc cagcgccgtg agcacgacgg
     2341 ccacgcccag catcgcgagg gcgccggggt gggtgacgtc gaggagatcc aggttggcct
     2401 gggcgccgac gctcacgaag aagaccggcg tcagcaccgc cgcgaccggg cgcacgcggt
     2461 cctcgatgac gtggaactgg ttcgtccccg acaggatgag cccggcggcg aaggcgccga
     2521 tgatcatcgc cgagcccgcc gcatcagcga gcgcggccat caggagcgcg aaggcgatgg
     2581 cgaagaccac cagcacgtgg cggacccgca tcgcctcgat ccggtcgaag agccgcggtg
     2641 cgagcttgag tcccagcacc acggccacga cgaggaaccc gatggccacc aggagcacgc
     2701 ggaacccggc gcccaccccg accgcggtgc ctcccgcgat ggtcgacacg accgagagga
     2761 tcacgagccc gaggacgtcg tcgatgacgg ccgcacccag gatgaccttg gcctcctcgg
     2821 tgtcgaggcg ccgcaggtcg gcaaggacgc gcgccgtgat gccgaccgac gtggcggtca
     2881 gcgtggcgcc gacgaagacc gccgccaccg cgagcggcac ggtcgatgcc accagctcgt
     2941 ggggcccgtg ggcccagtag aggtagccga gcccgaaggg gaccaacacc cccgccacgg
     3001 ccaccgatgc cgcggcggtg ccgacccgga acatctcctt caggtcggtc tcgaggccga
     3061 tctcgaagag aagcatcact acgcccacct cggccagcag gtggacgaac ga
//
LOCUS       DASUAP010000376         6067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_762074, whole genome shotgun sequence.
ACCESSION   DASUAP010000376 DASUAP010000000
VERSION     DASUAP010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762074"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..793)
                     /gene="thyX"
                     /locus_tag="VFU45_10100"
     CDS             complement(41..793)
                     /gene="thyX"
                     /locus_tag="VFU45_10100"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU5359462.1"
                     /translation="MDFLRAPRVTLLARPQFVEPAHLAVKWNGPGTDGEKLAEFAGRL
                     CYMSQHNPAGRTTADYLTNILKQGHGSVFEHSVYVLLLEGISRSCTHELVRHRAGFGY
                     SQLSQRYVDESHAAFVVPPAILGNEALEAAWRAQMTAAQTAYVAAVEGLMAQYAWVED
                     KVHRRKMAREAARSVLPNGTETKIVVSGNVRAWRTMLELRLGEGAEQEIRRLAVAVLR
                     TLQGEAPRLFQDFEIYQAPDAAEAGRVGFHKV"
     gene            complement(1148..2791)
                     /locus_tag="VFU45_10105"
     CDS             complement(1148..2791)
                     /locus_tag="VFU45_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5359463.1"
                     /translation="MPHHPSPPPIRGIFRTDLRARAAYAEGAGIFRIVPAAVARPEDV
                     EDLARLARWAHETRTPLVARGAGSAMGGGNVGSGVIVDLRRIGATSLLVDRGTLTART
                     GAAVTCGALEAAAAKHHLRLPPDPSSGRWATLGGMLSTNASGARSLRYGSVRRWALAA
                     ELVTAEGELVELRRGGMARDVAAIGRFRGNAWPAITAAATRIAERFPKTSKNSAGYAL
                     DHAVRSGDFLDLLIGAEGTLGFVTAITWQLDRIPAAHASLRVSVRELDQLADLVPALR
                     ALGPSTLELLDRSFLDVVRGSEAARNAGIEGNEAAVLLVELEGENAEEVDGLVLRMAD
                     AARPRASSVAMARTPAEEQQLAALRHAASPILAGMSDRRSVQVIEDACVPVERLGDYV
                     GAVRRISAAHDVQAVIFGHAGDGNVHVNLLPDVRTAGWERSVQAIFDEVTAVALSLGG
                     TPTGEHGDGRLRAPLLERVYGPEIVALFRKVKDDFDPRGILNPGIKLAAPGADGSLTD
                     LKMGSGVAPIPDDIAATLRDLERSGGYARVRLVVAGGGV"
     gene            2863..3672
                     /locus_tag="VFU45_10110"
     CDS             2863..3672
                     /locus_tag="VFU45_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HEU5359464.1"
                     /translation="MAQLPLVDPPRAKLIHIDVDRRLGHEWDEWDGKPLPNAGNYSSP
                     PALFVWWSAAALATSTGVAAALLFLLAPRLALLDAAVPRVLWLALAIVAAAAATWWVL
                     MALSVVARRPLLPERLAERGVFLWVMRATSRVAGWFGRRDWVENAAVKVYNVLASNRG
                     RKVGKGEMLLLIPRCLSKETLDGVLQVAGKYEVPVFVATRGQLARRVIRERRPRAVVA
                     VACERDMVSGLHDVAGKVPVLGLTMTLPAGPCKDAGLDLAQLEEWVKNYVV"
     gene            complement(3769..4239)
                     /locus_tag="VFU45_10115"
     CDS             complement(3769..4239)
                     /locus_tag="VFU45_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359465.1"
                     /translation="MKRIRVGAVLLAAAWLVACNPNTTRPAIMPSPGSLTLIVPLERG
                     EAIARLAQRLRDDSIPVARVDTLDAWLESPWMDSLGHVTTARPIGPNVIRLRGWTDPA
                     KVGSSVITVELSWRPYADPSVPQRELDRDVASTLPIAKRVGDVIAQLKKDYGGD"
     gene            complement(4257..5168)
                     /locus_tag="VFU45_10120"
     CDS             complement(4257..5168)
                     /locus_tag="VFU45_10120"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5359466.1"
                     /translation="MTHDPRHAALPFSHVAVIGAGQMGAGIAQVCATAGCHVTVRDVD
                     AEQAAKGKRAIEASTAKLVEKGKLTAGDRDAALARISFTDALGAAAQADLVIEAIVED
                     LEAKRALWRELDELAPPSSVFATNTSSLKVADQAEVTDRPDRFLGLHFFNPVPLMGLV
                     EVVSTAATDPGIVASATDFVRTLGKEPVLARDTTGFIVNVLLIPYLLDAIRLLERGGG
                     TIGDIDTAMKLGAGYPMGPFTLLDTVGLDTMLRTTRALFAEFGEPRLQAPAMLERLVA
                     AGRLGRKSGKGFYDWSVVPAVPVESEK"
     gene            complement(5306..>6067)
                     /locus_tag="VFU45_10125"
     CDS             complement(5306..>6067)
                     /locus_tag="VFU45_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359467.1"
                     /translation="LQTGEHELGDDLSREDILKLGREVGIPTRYLEQAMIEERVRTTE
                     GALGGAWDKLVGPATVKAMRVVRGTPEQAQRALIAWLDEHELLAVAREQPGRVLWEPV
                     GGFHAAMRRAGAVMGGVARPFMLARCRQVSAAITALEDGYCHVSLEADIGKARGAMIG
                     GATALVAVGAAAAAILSLLHAFLPLALVPVPFGLVGGWAVLRGYPPVVARTQLGLDRA
                     LDALEQGAAAATPSLPAGRPGLLDAVLGEVRKAIK"
BASE COUNT          820 a         2157 c         2151 g          939 t
ORIGIN      
        1 cgcccctacg ccctacgccc taagcgctaa gcgctacccc ctacacctta tggaacccca
       61 cccgtcccgc ctccgccgcg tcgggcgcct ggtagatctc gaagtcctgg aagagtcggg
      121 gcgcctcgcc ctggagggtg cggaggaccg ccaccgccag ccggcggatc tcctgctccg
      181 cgccctcgcc gaggcggagt tccagcatcg tacgccaggc gcggacgttc ccgctcacca
      241 cgatcttggt ctcggtgccg ttggggagga cgctccgcgc cgcctcgcgg gccatcttcc
      301 gccggtggac cttgtcctcc acccaggcgt actgcgccat cagcccctcc accgccgcca
      361 cgtacgccgt ctgcgccgcg gtcatctgcg cccgccacgc cgcctcgagc gcctcgttcc
      421 ccaggatggc gggggggacg acgaatgcgg cgtggctctc gtccacgtaa cgctgggaga
      481 gctggctgta gccgaacccc gcccggtggc gcaccagctc gtgggtgcag gagcgcgaga
      541 tcccctcgag gaggagaaca tagaccgaat gctcgaacac cgacccgtgg ccctgcttga
      601 gaatgttggt caggtagtcc gcggtggtcc gccccgccgg gttgtgctgg ctcatgtagc
      661 agagccgtcc ggcgaactcc gccagcttct cgccatcggt gcccggtccg ttccatttga
      721 ccgcgaggtg cgccggctcg acgaactgcg gacgggccag cagggtgacg cggggcgcgc
      781 ggaggaagtc catggagtcg tgggtcgtga gtcgtgggtc gtgggtcggg cgaggcggaa
      841 atgtaggcgg taaggcggta agacggtaag gcggtaaggc ggcaaagggg tagcggggga
      901 agccggtgag gcggtaggac cggaaggcgg taagggcgac cgggagctgt cggaccatcg
      961 gtgtcatcta gagggagccg tcagcccatc ggtgtcatcc tgcctgtcct gagcgcagcg
     1021 aaggaaggga gcgcagcgac cgaaggatct ctcggccagc cttgccctca ttccctctcc
     1081 ttttccctct tccctcttcc ccttttcact cttccctttt cacttctcac ttctcacttc
     1141 ctactcctca caccccccct cccgccacca ccaaccgaac ccgcgcatac ccgccgctcc
     1201 gctcgaggtc ccggagcgtc gccgcgatgt cgtcggggat gggcgcgacg ccgctcccca
     1261 tcttgaggtc cgtcaaggat ccatcggcac ccggagcggc gagcttgatc ccggggttga
     1321 ggatccccct ggggtcgaag tcatccttca ccttccggaa gagcgccacg atctccggcc
     1381 cgtagacccg ctccagcagc ggcgcgcgga gtcgcccgtc gccgtgctcg ccggtcgggg
     1441 tgccgccgag ggagagggcg accgccgtca cctcgtcgaa gatcgcctgg accgaccgct
     1501 cccaccccgc ggtccgcaca tcgggcagga ggttgacgtg gacgttcccg tcccccgcgt
     1561 gcccgaagat gaccgcctgg acgtcgtgcg ccgcgctgat ccgtctcacc gcccccacgt
     1621 agtcgcccag gcgttccacc ggcacgcagg catcctcgat cacctggact gagcgccggt
     1681 cgctcatccc ggccaggatc ggactggctg cgtggcggag cgctgcaagt tgttgctctt
     1741 ccgccggtgt gcgtgccatc gcgacggagc tcgcccgtgg ccgtgcggcg tccgccatcc
     1801 ggaggacgag cccgtccacc tcttcggcgt tctccccctc cagctcgacc aggagcactg
     1861 ccgcctcgtt gccctcgatc ccggcgttcc gtgccgcttc gcttccccgg accacatcga
     1921 ggaagctccg gtcgaggagt tcgagggtcg aaggtcccag ggcacggagc gcggggacga
     1981 ggtccgccaa ctggtcgagt tcgcggaccg agacccggag gctcgcgtgc gccgcaggga
     2041 tccggtcgag ctgccaggtg atcgccgtga cgaagccgag cgtcccctcc gcgccgatca
     2101 ggaggtcgag gaaatcgccg ctccggaccg cgtggtcgag tgcgtacccc gccgagttct
     2161 tggatgtctt cgggaatcgt tcggcgatgc gcgtggcggc ggcggtgatg gccggccacg
     2221 cgttcccgcg gaaccgtccg atcgcggcga catcccgcgc catcccaccc cgccgcagtt
     2281 cgacgagctc cccttcggcg gtcaccagct ccgccgccag cgcccaccgc cgcacgctgc
     2341 cgtagcggag gctccgcgca ccggaggcgt tggtggagag catccccccc agcgtggccc
     2401 agcggccgct cgacgggtcg ggggggagcc gcaggtggtg ctttgcggcc gcggcctcga
     2461 gtgccccgca ggtcaccgcg gcgccggtcc gggcggtgag cgtgccccga tccaccagga
     2521 gcgaggtggc gccgatccgt cggaggtcga cgatcacccc tgacccgaca ttgccgcccc
     2581 ccatcgcgct gcccgcgccc cgtgccacga gcggggtgcg ggtctcgtgg gcccatcgcg
     2641 ccaggcgggc gaggtcttcc acgtcctcgg ggcgcgccac ggcggccggc acgatccgga
     2701 agatgccggc gccttccgcg taggcggcgc gcgcgcggag gtcggtgcgg aagatcccgc
     2761 ggatcggagg gggggagggg tggtgcggca cgcgcgaaat ctgtcgcctt ggccctcccg
     2821 ccgcgagagg cgcgcggggg gcaggggggc tattttcctt ccatggccca gctcccgctc
     2881 gttgacccgc cccgcgccaa gctcatccac atcgacgtcg accgtcggct cggccacgag
     2941 tgggacgagt gggacgggaa gcccctcccc aacgccggca actactcgag cccgcccgcg
     3001 ctcttcgtgt ggtggtcggc cgcggcgctc gcgacctcga ccggcgtggc ggcggcgctc
     3061 ctcttcctgc tggccccgcg ccttgcgctc ctcgacgccg ccgtcccgcg ggtgctctgg
     3121 ctggcgctcg ccatcgtggc cgcggcggcg gcgacctggt gggtgctgat ggcgctctcg
     3181 gtcgtggcgc gacggccgct cctgcccgag cggctggccg aacggggggt gttcctctgg
     3241 gtgatgcggg cgacgtcacg ggtggcgggg tggttcgggc gccgcgactg ggtcgagaat
     3301 gcggcggtca aggtctacaa cgtcctggca tcgaatcggg ggcggaaggt ggggaagggg
     3361 gagatgctcc tcctcatccc gcgctgcctg tcgaaggaga ccctcgacgg cgtgctgcag
     3421 gtggcgggga agtacgaggt gccggtgttc gtggccaccc gggggcagct cgcccggcgg
     3481 gtcatccgcg agcggcggcc gcgcgccgtg gtggccgtgg cctgcgagcg ggatatggtg
     3541 agcgggctcc acgacgtggc ggggaaggtg ccggtgctgg ggctcacgat gacgctcccg
     3601 gcgggaccgt gcaaggatgc ggggctcgac ctggcgcagc tcgaggagtg ggtcaagaac
     3661 tacgtggtgt gagaagtaag aagtgagaag tgaatagtga gaaggggctc cgcgtggtgc
     3721 ggggccccgt ttgcgtatcg atcgaatatg tcgtgggggc ggcgtaatct agtctccccc
     3781 gtaatccttc ttgagctgcg cgatcacgtc gcccacccgc ttcgcgatcg ggagggtgga
     3841 tgccacgtcc cggtcgagct cgcgctgggg caccgacggg tcggcgtagg ggcgccagct
     3901 cagttccacg gtgatgaccg acgatccgac cttggccggg tcggtccacc cgcggagccg
     3961 gatgacgttg gggccgatcg ggcgcgcagt ggtgacgtgt cccagcgagt ccatccacgg
     4021 cgattcgagc caggcatcga gcgtgtccac ccgggccacc gggatgctgt cgtcccggag
     4081 gcgctgggcc agcctggcga tcgcctcgcc ccgctccagc gggacgatga gcgtgagcga
     4141 tcccggcgac ggcatgatcg ccggccgggt ggtgttcggg ttgcatgcga ccagccacgc
     4201 ggcggccagc agcaccgctc ccactcggat cctcttcacc tttcacttct cacttttcac
     4261 ttctcacttt cgaccggcac cgccggcacc accgaccagt cgtagaaccc cttgccgctc
     4321 ttccgcccca gccgtcccgc ggcgaccagt cgctcgagca tcgccggggc ctgcaggcgc
     4381 ggttccccga attcggcgaa cagcgcgcgg gtggtgcgga gcatggtgtc gagccccacg
     4441 gtgtcgagca acgtgaacgg gcccatcggg tagccggcgc cgagcttcat ggcggtgtcg
     4501 atgtcgccga tggtgccccc gccgcgctcg aggagccgga tcgcgtcgag caggtagggg
     4561 atgaggagca cgttgacgat gaagccggtg gtgtcccgcg ccaggaccgg ctccttgccg
     4621 agtgtgcgga cgaaatcggt ggcgctggcg acgatgccgg ggtcggtggc cgcggtgctg
     4681 accacttcca ccagccccat cagcggcacg ggattgaaga agtggaggcc gaggaaccgg
     4741 tcggggcggt cggtgacctc cgcctggtcg gcgaccttga ggctcgacgt gttggtcgca
     4801 aagaccgaac tcggcggggc gagctcgtcg agctcacgcc agagggcgcg cttggcctcg
     4861 aggtcctcga cgatggcctc gatcacgagg tcggcctgtg cggcggcgcc gagcgcgtcg
     4921 gtaaaggaga tgcgcgcgag cgctgcgtca cggtcgccgg ccgtcagctt ccccttctcg
     4981 accagcttgg cggtcgacgc ctcgatggcc cgcttcccct tggccgcctg ctcggcatcg
     5041 acgtcccgca cggtgacgtg gcaccccgcc gtcgcgcaca cctgcgcgat cccggcgccc
     5101 atctggccgg caccgatcac ggcgacgtgg gagaatggga gggcggcgtg tcgtgggtcg
     5161 tgagtcacgg gtctcgggtc tcgggtcgtg agtcgttgga ttcgggtcgt gggtcaaagg
     5221 tcgcaggtcg ccgggcttac ttctacctct cgcgtctcac gactcatgac tcgtgactca
     5281 cgactcatga ctcacgtctc acgactcact tgatcgcctt ccgcacctcg ccgagcaccg
     5341 catcgaggag ccccgggcgg ccggccggca gcgacggcgt ggcggcagcg gcgccctgtt
     5401 ccagcgcatc gagcgcgcgg tcgaggccca gctgggtccg tgccacgacc ggcgggtatc
     5461 cgcgcagcac ggcccacccg cccaccaggc cgaacggcac cggcacgagc gccagcggca
     5521 ggaaggcgtg gagcagcgag aggatggccg ccgcggcggc gcccacggcc acgagcgccg
     5581 tcgcgccgcc gatcatcgcc ccccgcgcct tgccgatgtc ggcctcgagg gaaacgtgac
     5641 agtagccgtc ctcgagcgcc gtgatcgcgg ccgacacctg gcggcaccgg gccagcatga
     5701 aggggcgtgc caccccgccc atcaccgcgc cggcccggcg catcgcggcg tggaaccccc
     5761 ccaccggctc ccagagcacc cggcccggct gctcccgcgc caccgccagg agctcgtgct
     5821 cgtcgagcca cgcgatgagc gcgcgctgcg cctgttccgg cgtcccgcgc accacccgca
     5881 tcgccttgac cgtggcgggg ccgaccagct tgtcccaggc gccgccgagg gcgccttcgg
     5941 tggtccggac ccgctcctcg atcatcgcct gctcgaggta gcgggtgggg atccccacct
     6001 cgcggccgag cttgaggatg tcctcccgcg agaggtcgtc gccgagctcg tgctcgccgg
     6061 tctgcag
//
LOCUS       DASUAP010000377         5906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1307501, whole genome shotgun sequence.
ACCESSION   DASUAP010000377 DASUAP010000000
VERSION     DASUAP010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307501"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VFU45_10130"
     CDS             complement(<1..1326)
                     /locus_tag="VFU45_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5359468.1"
                     /translation="MLKNLTIGRRLGLALGVLLILMLAVAGMGIWGVRQASDLTSSIL
                     DNDAVAQVAALQAQIQVLELRRSEKDVLLNMADTARSHSYAAKWADSHNALEAQVKIL
                     TPLAEAGVERREVDSMTLDLAAYDSGFAFVRQQIKEGKLRTPAAANLVMERFKTPIHE
                     LEAQAANVAAAQTGTTQAASAQLHATEKRLILLTLITVMVVIGLGAVLSFAITRSITV
                     PMLAAVGAANRVAEGDVSVDLQVDRRDETGQLLAAMRRMIDSIRGTADAASRIADGDL
                     TVQIQPKSDRDLLGNSLQQMVAKLAQVIGEVRAGAEALSGASAQVSATSQGLSQGTSE
                     QAASVEETTSSLEQMSASITKNADNSSQMESMAVKGAADAGDSGKAVTDTVVAMKDIA
                     DKISIIEEIAYQTNLLALNAAIEAARAGEHGRGFAVVATEVRKLAERSQS"
     gene            complement(1320..2972)
                     /locus_tag="VFU45_10135"
     CDS             complement(1320..2972)
                     /locus_tag="VFU45_10135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEU5359469.1"
                     /translation="MTQLSDQDRELLLQAFFAQTAEALDGLESSVVALEQAPHDDELL
                     NGIFRAAHSLKGDALIVGFRHVAEVAHALEDLLSGLRDHERALDAGVASAVLGAVDAL
                     RSMLAGAARDGIEGTGLKAAAIELLRGVMVRGPHEDAAAATEGAGSLAPSPAELRTLR
                     VDVAKLDKMLDLAGEMAIARGRTAGLLERVAGPLHDELVELHREAERLQLELQEQVMR
                     ARMVAIGPFLRRYARTVREMARGAGKEATLEIDGGEAEVDTRVLELLRDPLTHMIRNA
                     VDHGLESPEVRAAAGKPAAGRITLAAAHEAGRIVLRVRDDGAGFALDRIRRRAAETGL
                     ATDPELLPQGELLRLVFEPGFSTAESVTDLSGRGVGMDVVRRNIESLRGTIDIDTVEG
                     SGSEITIRLPLTLAIIDGFGVGVGEERYVLPLTSVLECLTLPAARREDGAGAGVLDLR
                     GAPLPYVRLRDLFALGGQAPAREHVVVVEHEGGRTGLVVDTLHGEGQVVIKSLGRGLD
                     APAGLAGSTILGSGRVALILDVPTLVRRAQARVTHTHEGSPC"
     gene            complement(2972..3526)
                     /locus_tag="VFU45_10140"
     CDS             complement(2972..3526)
                     /locus_tag="VFU45_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEU5359470.1"
                     /translation="MTRSPVQFGLGDLESLLGSLVGDAIPAEAGDRPPAGRAAFVHTG
                     DVFASATSTVASTILGSCVAVCLWDARAGVGGINHFLLPWEVENGSSSPRYGNVAIRT
                     LIARLEALGSRRTQLLAKVFGGASVLETAAPAADALGARNVAFAEQALAEAGIPIVSR
                     DVGGTRGRKLYFRTDDGSAWVRKL"
     gene            complement(3523..4110)
                     /locus_tag="VFU45_10145"
     CDS             complement(3523..4110)
                     /locus_tag="VFU45_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5359471.1"
                     /translation="MNAELTPDPVTGTDARIAKLGADLRQAISRALVAEHRVGRLAQL
                     LAAVAELHSGEDAAAVVTTVKEIVANLVGCEEMGVFAVQRPGPVLTYVDGIGIDANEF
                     ATIPAATGPIAAALASSVVLVPGHPESATVHGRPVTVCVPLVTGGRVFGLVVLFHLVL
                     QKPRLNADDLELLRFLSDHASMALGRVPGFPGAAS"
     gene            complement(4113..4487)
                     /locus_tag="VFU45_10150"
     CDS             complement(4113..4487)
                     /locus_tag="VFU45_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5359472.1"
                     /translation="MTTPKTILVADDSATALMMHKMILGTHGFRLLTANDGLEAVNSA
                     TANLPDLILMDVVMPRMTGFEAVRALKAKDETRDIPIIMVTTRAEPKVIETCYSAGCH
                     DYVNKPVDGPVLLAKVRSILGN"
     gene            complement(4484..5269)
                     /locus_tag="VFU45_10155"
     CDS             complement(4484..5269)
                     /locus_tag="VFU45_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5359473.1"
                     /translation="MLLVDQLLAPGGLVSTFQPIVRIVGSECRVEEVEGLVRGAAGTN
                     AARSAVLFEYARRKRIEPQIDRACTELVCRSAAPIAGRGIGLSINVHAATLARDAEFP
                     EFLLAQAADAGFPGDHLTVELVEHSSHWNREGALAAIDSLRGAGVRIALDDVGRGHSN
                     IDLLLDVRPDVVKLDRYFVVGSSQCDMRRAVLASFRDMAAGLGAVVVAEGVETAEDLA
                     TVVEAGVVVVQGWYFGHPVASVADAASPYGVRQLPAALSRGPR"
     gene            complement(5269..5679)
                     /locus_tag="VFU45_10160"
     CDS             complement(5269..5679)
                     /locus_tag="VFU45_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5359474.1"
                     /translation="MIASIQVSTEWGAPQILIVDDEEQILLAMQDYFTAIGCQVTGAR
                     EVEEAEALLATTAFDVVLADVRLGGVHGNEGLRLLSFVRERSLSTRVVLMTAYGAADV
                     DVEARRRGADLFLHKPIPLSELARRALRLVGVRT"
BASE COUNT          822 a         2122 c         2131 g          831 t
ORIGIN      
        1 cgactggctc cgttcggcga gcttccgcac ctcggtggcc acgaccgcga agccgcggcc
       61 gtgctcgccg gcgcgcgcgg cctcgatggc ggcgttgagc gcgagcaggt tggtctggta
      121 cgcgatctcc tcgatgatcg agatcttgtc ggcgatgtcc ttcatggcca ccacggtgtc
      181 ggtgacggcc ttgccgctgt cgccggcgtc ggcggcgccc ttgacggcca tcgactccat
      241 ctgggagctg ttgtcggcgt tcttggtgat cgacgcgctc atctgctcga ggctcgacgt
      301 ggtctcttcg accgacgcgg cctgttcgct ggtgccctgc gagagcccct gcgaggtggc
      361 cgagacctgg gccgaggcgc cactcagcgc ctccgcgccg gcgcggacct cgccgatgac
      421 ctgggccagc ttggccacca tctgctgcag cgagttgccg aggaggtcgc ggtcggactt
      481 gggctggatc tggacggtca ggtcgccgtc ggcgatccgg gaggcggcgt ccgcggtccc
      541 gcggatcgag tcgatcatcc gccgcatcgc ggcgaggagc tggccggtct catcccggcg
      601 gtccacctgc aggtccactg acacgtcgcc ctcggccacc cggttggcgg caccgaccgc
      661 cgcgagcatc ggcacggtga tgctgcgggt gatggcgaag ctgaggaccg cgccgaggcc
      721 gatgaccacc atcaccgtga tgagggtgag gaggatcagg cgcttctcgg tggcgtggag
      781 ctgggcgctc gccgcctggg tggtgccagt ctgggcggcg gcgacgttgg ccgcctgggc
      841 ctcgagttcg tggatcgggg tcttgaagcg ttccatcacg aggttggccg ccgccggcgt
      901 gcggagcttc ccctccttga tctgctgccg cacgaacgcg aagcccgagt cgtacgcggc
      961 caggtcgagg gtcatcgagt cgacctcccg gcgctccacg cccgcctcgg cgaggggggt
     1021 gaggatcttg acctgcgctt cgagcgcgtt gtgggagtcg gcccacttgg ccgcgtagga
     1081 atgcgagcgg gcggtgtccg ccatgttgag gagcacgtcc ttctccgagc ggcgcagttc
     1141 gaggacctgg atctgcgcct ggagcgcggc cacctgggcc acggcgtcat tgtccaggat
     1201 ggacgacgtg aggtcgctcg cctgccgcac gccccagatg cccatgccgg ccacggcgag
     1261 catcaggatg agcaggacgc cgagcgccag tccgagccgc cggccgatgg tcaagttctt
     1321 cagcatggtg atccctcgtg tgtgtgtgtg acccgggcct gcgcccgccg caccagcgtc
     1381 gggacgtcga ggatgagggc cacgcggccg ctccccagga tcgtcgaacc cgcgagcccg
     1441 gccggggcgt cgagcccccg tccgagcgac ttgatgacca cctggccctc gccgtggagc
     1501 gtatccacca cgagcccggt gcgtccgcct tcgtgttcca ccaccaccac gtgctcccgc
     1561 gccggggcct ggccgccgag cgcaaagagg tcgcggagcc gcacgtacgg gagcggcgcg
     1621 ccgcggaggt cgaggacgcc ggcccccgcc ccgtcctcgc ggcgggcggc gggcagcgtc
     1681 aggcactcga gcaccgacgt gagcggcagg acgtagcgct cctcccccac gcccaccccg
     1741 aaaccgtcga tgatcgcgag cgtgagcggg agccggatcg tgatctcgct ccccgacccc
     1801 tcgaccgtgt cgatatcgat ggtgccgcgg agcgactcga tgttccgacg caccacgtcc
     1861 atcccgacgc cgcgccccga caggtcggtg acgctctcgg cggtcgagaa ccccggctcg
     1921 aacacgagcc gcaggagctc accctgtggc agcagctccg ggtccgtggc cagcccggtc
     1981 tccgccgccc ggcggcggat ccggtcgagc gcgaaccccg cgccgtcgtc gcggacccgg
     2041 agcacgatcc gcccggcctc gtgcgccgcc gcaagggtga tccgcccggc ggcgggcttc
     2101 cccgcggccg cccgcacctc cggcgactcg agcccgtggt cgacggcgtt gcggatcatg
     2161 tgggtgagcg ggtcgcgcag gagctcgagc acccgcgtgt cgacctcggc ctcgcccccg
     2221 tcgatctcga gcgtggcctc cttgccggcg ccgcgggcca tctcgcgcac ggtgcgggcg
     2281 tagcgccgca ggaaggggcc gatggccacc atccgcgcgc gcatcacctg ctcctgcagc
     2341 tcgagctgga gccgctccgc ctcgcggtgg agttcgacca gctcgtcgtg gagcgggccg
     2401 gccacccgct ccaggagccc ggcggtccgg ccccgggcga tcgccatctc cccggccagg
     2461 tcgagcatct tgtcgagctt ggccacgtcg acccgcaggg tgcggagctc cgccgggctc
     2521 ggcgccagcg aacccgcccc ttcggtggcc gcggccgcat cctcgtgcgg accccgcacc
     2581 atgacgccgc gcagcagctc gatcgccgcc gccttgaggc cggtcccttc gatgccgtcc
     2641 cgggcggcgc ccgcgagcat gctccggagc gcgtccaccg ccccgagcac cgcggaggcc
     2701 acgccggcat cgagggcgcg ctcgtggtcg cggaggccgg agaggaggtc ttcgagcgcg
     2761 tgcgccacct ccgccacgtg gcggaagccg acgatgagcg catcgccctt gagcgagtgc
     2821 gcggcgcgga agatcccgtt gaggagctcg tcatcgtgcg gcgcctgctc cagcgccacc
     2881 accgagctct cgagcccgtc gagcgcttcc gccgtctggg caaagaacgc ctgcagcagg
     2941 agctcgcggt cctggtcgga gagctgggtc atcagagctt cctcacccag gcgctgccgt
     3001 cgtcggtgcg gaagtagagc ttccgccccc gggtgccgcc cacgtcccgc gacacgatcg
     3061 ggatgcctgc ctcggccagg gcctgctcgg cgaatgccac gttccgggca ccgagggcgt
     3121 ccgccgccgg cgccgccgtc tcgaggacgc tggcaccgcc gaagaccttg gccagcaact
     3181 gcgttcggcg cgacccgagg gcctcgagcc gcgcgatgag cgtgcggatg gccacgttgc
     3241 cgtaccgggg ggaggagctc ccgttctcca cctcccacgg gagcaggaag tggttgatcc
     3301 cgcccacgcc ggcccgggca tcccacaggc acaccgccac gcaggagccg aggatcgtgc
     3361 tcgccaccgt ggacgtggcg ctggcgaaca cgtcgccggt gtggacgaag gccgcgcgcc
     3421 ccgcgggcgg ccgatcgccg gcctccgccg ggatcgcgtc gcccacgagc gagccgagga
     3481 gcgactcgag gtcgcccagg ccgaactgga ccggggagcg cgtcatgacg cggcgcccgg
     3541 gaaaccgggg acccgcccga gcgccatcga ggcgtggtcg gagaggaagc gcagcagctc
     3601 gaggtcgtcg gcgttgaggc gcggcttctg cagcaccaga tggaacagca cgacgagccc
     3661 gaacacccgg ccgccggtca ccaggggcac gcacaccgtc accggccggc cgtgcaccgt
     3721 ggccgattcc ggatggcctg ggaccagcac gaccgacgac gccaacgcgg cggcgatcgg
     3781 tccggtcgcg gcggggatgg tcgcgaattc gttcgcgtcg atcccgatgc cgtcgacata
     3841 ggtcagcacc gggcccggcc gctggaccgc gaagacgccc atctcttcgc acccgaccag
     3901 gttggcgacg atctccttga ccgtcgtcac gaccgccgcg gcatcttcgc cgctgtgcag
     3961 ttcggccacg gcggccagga gctgcgcgag ccggccgacg cggtgctcgg ccaccagcgc
     4021 gcgcgagatg gcctggcgga ggtcggcgcc cagcttggcg atccgcgcgt ccgtgccggt
     4081 caccgggtcg ggagtgagtt cggcgttcac ggtcagttcc ccaggatgct gcggaccttg
     4141 gccagcagca ccggtccgtc gaccggcttg ttgacgtagt cgtggcagcc ggccgagtag
     4201 caggtctcga tgaccttggg ctcggcgcgg gtggtgacca tgatgatcgg gatgtcgcgg
     4261 gtctcgtcct tcgccttgag cgcccgcacc gcctcgaacc cggtcatccg cggcatcacc
     4321 acgtccatca ggatgaggtc gggcaggttc gccgtggcgg agttgaccgc ctcgagcccg
     4381 tcgttcgcgg tcaggagccg gaacccgtgg gtgccgagga tcatcttgtg catcatcagg
     4441 gcggtggcgg aatcgtccgc caccaggatc gtcttgggcg tcgtcatcgg ggacctctcg
     4501 agagcgcggc cggcagctgc cggacgccgt agggggaagc ggcatcggcg accgacgcca
     4561 ccggatgtcc gaaataccag ccctgcacca ccaccacgcc cgcttcgacg acggtcgcga
     4621 ggtcctcggc ggtctcgacg ccctcggcca ccaccacggc acccaggccc gcggccatgt
     4681 cgcggaagga cgcgagcacc gcccggcgca tgtcgcactg ggaggacccg accacgaagt
     4741 accggtcgag cttgaccaca tcgggccgca cgtcgagcag gaggtcgatg ttcgaatggc
     4801 cgcgccccac gtcgtcgagc gcgatgcgga ccccggcgcc gcgcaggctg tcgatcgcag
     4861 cgagcgcgcc ctcgcggttc cagtgggacg aatgttccac cagctcgacg gtcaggtggt
     4921 cgccggggaa gcccgcgtcc gccgcctgcg cgaggaggaa ctcgggaaac tccgcatccc
     4981 gggccagcgt ggcggcgtgc acgttgatgg agagtccgat gccccgcccg gcgatcggcg
     5041 ccgccgaacg gcacaccagt tccgtacacg cccggtcgat ctgcggctcg atccgcttcc
     5101 ggcgggcgta ctcgaagagc accgcggaac gggcggcgtt ggtgccggcc gcgccgcgca
     5161 ccagcccttc gacctcctcg acccggcact ccgagccgac gatgcggacg atgggctgga
     5221 acgtggacac gagcccgccg ggcgcgagca gctggtcgac gaggagcatc aggtgcgcac
     5281 cccgacgagt cggagggccc gccgcgccag ctcggagaga gggatcggct tgtggaggaa
     5341 gaggtcggcg ccacggcgcc gcgcttcgac gtcgacgtcg gcggcgccgt aagcggtcat
     5401 cagcaccacc cgggtgctga ggctgcgctc ccgcacgaag gagaggagcc ggagcccctc
     5461 attgccgtgc accccgccca ggcgcacgtc ggcgagcacc acgtcgaacg cggtcgtggc
     5521 caggagcgcc tcggcctcct cgacctcgcg tgcgccggtg acctggcagc cgatggcggt
     5581 gaagtagtcc tgcatggcaa gcaggatctg ctcctcgtcg tcgacgatga ggatctgggg
     5641 cgcgccccac tcggtggaga cctggatcga tgcgatcatc ggcgggcgca aaggcaatcg
     5701 gcgtaccagc acacgccgga gcgcctgcgt ccgcggtcat ctcacgtgtt cacagggagt
     5761 tgcgcggcgg cgggaggcgg atcgagcgcg gcgcgggagg gtccgtatgc cggagtcgtc
     5821 cttccggcgt ctagacgccg gacggcccga gccccagcgt cttgatccgc tggtagaggg
     5881 tgctgcgggg gacgtcgagg agcttc
//
LOCUS       DASUAP010000378         8464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_408788, whole genome shotgun sequence.
ACCESSION   DASUAP010000378 DASUAP010000000
VERSION     DASUAP010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408788"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..940
                     /locus_tag="VFU45_10165"
     CDS             251..940
                     /locus_tag="VFU45_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEU5359475.1"
                     /translation="MTETPRRRLAHQVIEQLQRWISLGDLSPGDRFPTEGELTARLGV
                     SRTTLREAVGVLAHSGVLEVRQGDGTYVGSAPPAGDPLGQRLRRAAALEVYEVRRLLE
                     LETARLAAERRTDEDVVALRHHLAERSAARASGNAAAFVTADVALHLAIAMATQNAVL
                     ADLYRSFTGALHGAITDVVHDPDLHEDTAALHEALVNAIADGHAADAVAATDRLLEVD
                     ARELRGTLGRS"
     gene            943..3618
                     /locus_tag="VFU45_10170"
     CDS             943..3618
                     /locus_tag="VFU45_10170"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5359476.1"
                     /translation="MCGARASRRLVSLAALPCVLATLASAQSVHPDAPRIARAAQRRG
                     PIVLDGALDDDAWRDAPSTGDFRQQDPDPGVPATQRTEIRFLYDADALYIGARMYDTE
                     GAAGVTTRLVRRDDDPNSDYLTLVLDTFHDHKGRTVFEVNPSGVKDDSRGLGTANPDR
                     SWNAVWNVATRIDSLGWTVEIRIPFSQLRFPTDPVQTWGLQVWRYEQRTNETSQWSYW
                     TKREAGGPPFFGHLTGLHVPHPGRHLELSPYGVAARDDQAATAQGPADTYRGGADVTY
                     LPTPDLSLNATFDPDFGQVEADPAVLNLSAFETFYPEKRPFFVEGAGLFQFGVATCFT
                     CGDAPGLDLFYSRRVGRPPQLRPAGAVLAVPAATRIIGAVKLLGQQAGGLTYGVIDAV
                     TAPERAAVADTTGKVALTPVEPLTNYLVARAKWAPPSGDLTLGGMATAVTRSPSTPAL
                     TALLARNALTGGTDLDWWWRRHTYHLETSLAASYVSGDTSALRRIQESSTHYFQRPDR
                     GARGGIFGDRFDPSATGLAGYAAYARIAKDAGEWMWEGALDLRSPGFEINDLGYLPTA
                     DAISAFANLERNFTLPTRSYRSISLVAGVEEQVNYDRDLVERQFHVAGNGLLLNNWVI
                     SAGYAASPPLLDDRRTRGGPVVMVSGIRSDSLSLTTDVRAPVYLNVTGSSGAAADGGG
                     AYAARAGLTFKPRANIAVTVTPSVSRATDQVQYVATVPDATAAAFFGHRYVFARAVQR
                     TAAVEVRLAATFTPTLSLDLYAQPFLASGDYSGFKEYDAPRSLARSEYGRDRGTIAFS
                     TAGGPHYTIDPDGAGPAPSFVIADPDFTRYSWRSNAVVRWEYRPGCVLYLIWTQARSG
                     SAPTGDIQLPRDANALFRSPADNILLIKASYWFAR"
     gene            3660..4043
                     /locus_tag="VFU45_10175"
     CDS             3660..4043
                     /locus_tag="VFU45_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5359477.1"
                     /translation="MRHWKMLSILSLVAVTSAAAGPRSPTVVAVAMVRDATGATYFAP
                     SNITAQPGDTLRFIVTAGMHDVDFAADSNPAGVRLPAATALIEHPGEAVNVVVALPPG
                     TYYFQCDPHVMRGMTGHLTVEAPGR"
     gene            4275..4913
                     /locus_tag="VFU45_10180"
     CDS             4275..4913
                     /locus_tag="VFU45_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359478.1"
                     /translation="MHLPSVSLRRSWPLLPLLLVGCVTARLTDVWKDPGLPAAPLTHL
                     MVVSLAPSEVLRRTWEDAMVAGLQGHGVEATPSYDVWPDGPPEIDSLAAEVQREAFDG
                     VVLTHPADVQTTRTYVHGQTTLVPTKVYDKWKQHYVTYYRRVETPGHVEVHTSLLVDT
                     DIWMAADQFRMVWTGTTRAMNPTSTDATNQAIIHAILPRLARQGVIPPARKQ"
     gene            complement(4921..6855)
                     /locus_tag="VFU45_10185"
     CDS             complement(4921..6855)
                     /locus_tag="VFU45_10185"
                     /inference="COORDINATES: protein motif:HMM:NF044459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU5359479.1"
                     /translation="MDHSADEALRAWFLGPRAENADLLERLMVEALRDHVFWRRNHHP
                     EDGLRIREADRRREGYEASVATLTQELLGLLADLKQDVPFFSGRYQAHMVGEQTIAAQ
                     VAYIATMLYNPNNVAGEAAPVTTRLELEVAAQLAAMMGYDPKAAWGHLSSGGTVANFE
                     ALWIARSLHYLPVALAGAAADLGLSLEVTLPDGRRAALADLPLWSLLNIDGGAALDLW
                     DALRAQVPAEAARTAVDAYSLASIGYQDYSLRLARHYGDQLPAAVVLVAATAHYSWEK
                     IARALGIGAHQLRLVPVDRTFRMDPDALWKRVRRCADSRTAILACVSVCGTTEEGAVD
                     RLDQVLEVRERARRELGLAFHVHADACFGGYASAITWGADGRRRSAEEIRTSTGIDWP
                     SEEWVRSITALGRADSISVDPHKLGFVPYPAGAFLLKDRRARELVAVDPPYLTATGRD
                     PDELFLGRCILEGSKPGAAAAAVWLSHRVLPLDERGYGHLISRTVVGAHRLHSALTAP
                     AAAAPFRVVTLPRPDLSIVCFALTHPDIGSLVELNALNEGIYERLSIQPGRPAPDYML
                     TRTRLQAPMYEGAVDAVLGELGVCTVDDWRAAGAEGLVVLRTTVMDPFLAEAGGVDHV
                     AGLIAAVRRAAAEAVREIRR"
     gene            complement(7075..8340)
                     /locus_tag="VFU45_10190"
     CDS             complement(7075..8340)
                     /locus_tag="VFU45_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEU5359480.1"
                     /translation="MLARLRSVTLSQWILISMVVGILIGWADHALWTGVDLGHWLNPV
                     AQIFLRMIKSIVVPLVFSALVVGIAGHGDDLKQVGRLAVKSIFYFEAVTTVALVIGLL
                     AVNVIRPGDGVNLAAASAQEGQQLAETTLSLSGVLEHTVPTSFFDAAAKNEVLQVVFF
                     AVLFGIGLAQLTGKRKETMLAFCDALTEVMFKFTALVMKFAPFGIGAAIAVTVGNSGI
                     GVLKNLLVLVATLYGALLAFVLLALLPIALAARVPIRGFWQWVKAPWLLAFSTASSEA
                     ALPLAMENLEKFGVPARIVAFVLPTGYTFNLDGSTLYLAVASVFVAQAAGIPLTVGQQ
                     VLMMLTLMLTSKGVAAVPRASLVILAGALATFHLPLEGVAVILGVDALMDMARTSVNL
                     VGNCLASCVMARWERDVTAGSQMDLRVGA"
BASE COUNT         1328 a         2992 c         2894 g         1250 t
ORIGIN      
        1 ggacggcgac gaaggccacc ctgttctccg ggtggcttcg tcgctggccc aaggtccgta
       61 cggagtgatg ctgagccagt caactggaac gccgcctggt ccggccggtc ccgggtgcca
      121 cgccgccgat caagggtcgc cagtcgatag ttgcagccaa cggataccgg tattaccgga
      181 actcgttgat ccgtgggcac ttgacttcag gttcttataa acatactatg atagtatgtc
      241 acgccaaaac ttgaccgaga ctccgcggcg ccgcctcgcc caccaggtca tcgagcagct
      301 ccagcgctgg atctccctcg gcgacctctc gcccggcgac cgcttcccca ccgagggcga
      361 actcaccgcc agactcggcg tcagccgcac cacgctccgg gaggccgtgg gggtcctggc
      421 ccacagtgga gtcctcgaag tgcgccaagg cgacggtacc tacgtcggct cggccccgcc
      481 ggccggcgac ccgctcggtc agcgcctccg gcgcgccgcg gcgctcgagg tatacgaggt
      541 tcggcgtctg ctcgagctgg aaacggcccg cctggcggcg gagcgtcgca ccgatgagga
      601 cgtcgtggcg ctccgccacc acctggccga gcggtccgcg gcccgcgcat ccggcaatgc
      661 cgccgcattc gtgaccgcgg acgtcgccct ccacctggcc attgcgatgg cgacgcagaa
      721 cgcggtcctc gccgacctct accggtcgtt cacgggagcc ctccacggcg cgatcactga
      781 cgtggtccac gatcccgatc tgcacgagga tactgccgcc ctccacgagg cgttggtgaa
      841 cgccatcgcc gacggccatg cggcggatgc agtagcggcg accgatcggc tcctcgaagt
      901 cgacgcccgg gaattgcgcg gcacgctcgg ccgttcgtag tgatgtgcgg cgcacgcgcg
      961 agccggcgcc tcgtcagcct ggccgccctc ccatgcgtgc tggcgacgtt ggcgtccgcc
     1021 caatcggtcc atcctgatgc tccccggatc gcgcgggcgg cgcagcggcg cggccccatc
     1081 gtgctcgatg gcgccctgga tgacgacgcc tggcgggatg ctccttccac cggcgacttc
     1141 cgccagcagg accctgatcc aggcgtcccg gccacgcagc ggaccgagat ccgattcctg
     1201 tacgacgccg atgcccttta catcggcgcc cggatgtacg acaccgaagg cgcagccggt
     1261 gtcaccaccc ggctcgtgcg ccgggacgat gatccgaact ccgactacct gaccctggtg
     1321 ctcgatacct tccacgacca taaggggcgc acggtcttcg aagtcaatcc gtccggcgtc
     1381 aaggacgact cgcgcggcct cggcacggca aacccggacc gctcgtggaa cgccgtctgg
     1441 aacgtggcga ctcgcatcga ctccctcggg tggaccgtcg agatccggat cccgttctcc
     1501 cagctgcgct ttcccaccga ccccgttcag acgtggggcc ttcaggtctg gcggtacgag
     1561 cagcgaacga acgagacgtc acagtggtcc tactggacca aacgcgaggc cggtggcccg
     1621 ccattcttcg ggcacctgac cggacttcac gtcccgcatc ccggccggca cctggaattg
     1681 agcccgtacg gcgtcgcggc ccgagatgat caagccgcca cggcgcaggg cccagccgac
     1741 acatataggg gcggcgctga tgtgacgtac ctccccacgc cggacctttc gctgaacgcg
     1801 acgttcgatc cggacttcgg ccaggtcgag gctgacccgg cggtgctgaa tctcagcgca
     1861 ttcgagacat tctatcccga gaagcgtccc ttcttcgtgg aaggcgccgg gctcttccag
     1921 tttggcgtgg ccacctgctt cacctgtggg gatgcgcccg gactcgatct gttctactcc
     1981 cggcgggtgg gacgcccacc gcaactgcgg ccggccggtg ccgtcctggc cgtccctgcc
     2041 gccacccgca tcattggcgc ggtcaaactg ctgggccagc aggccggggg gctcacctac
     2101 ggggtcatcg acgcggtgac ggcgccggag cgggccgccg ttgcagacac gacggggaag
     2161 gtggcgctga cgcccgttga gcccctcacc aactacctgg tcgcgagggc caagtgggcg
     2221 ccgccttccg gcgacctcac cctcgggggg atggcgaccg ccgtcacgcg ttccccgtcg
     2281 acgccggcgc tgaccgcgct gttggcgcgg aacgcactca ccggagggac cgacctggac
     2341 tggtggtggc gccggcacac ctaccatctt gagaccagcc tcgcggcatc gtacgtctcc
     2401 ggcgacacgt ccgcgctccg tcggatccag gaatccagca cccactactt tcagcgcccg
     2461 gatcgcggcg cacgcggggg gatcttcggc gaccgcttcg acccgtccgc cacgggcttg
     2521 gccggctacg ctgcctatgc gcggatcgcg aaggatgcgg gtgaatggat gtgggaaggc
     2581 gcgctcgatc tgaggagccc cgggttcgag atcaacgacc tggggtacct gccgacggcg
     2641 gatgccatct cggcgttcgc gaacctcgaa cgcaatttca cactgccgac ccggtcctat
     2701 cgctcgatct cgctcgtggc gggagtggag gagcaggtca actacgaccg tgacctggtg
     2761 gaacgtcagt tccacgtcgc cgggaatggg cttctcctca acaattgggt catttccgcg
     2821 ggctacgccg cgagtccgcc gctgctcgac gaccgccgga cgcgaggcgg gccggtggtg
     2881 atggtcagcg ggatcaggtc ggattcactc agcctgacca ccgacgtccg cgccccggtc
     2941 tacctgaacg tgaccggctc cagcggcgcc gccgccgacg gcgggggcgc ctacgccgcg
     3001 cgtgcgggcc tgacgttcaa gcccagggcc aacatcgcgg tgacggtgac gccgagcgtc
     3061 tcccgcgcga ccgaccaggt ccagtacgtc gccaccgttc cggacgccac agcggcagcg
     3121 ttcttcggac atcggtatgt gttcgcacgc gccgtgcagc gcacagcggc cgtcgaggtg
     3181 cgcctggcgg cgacattcac gccgaccttg tcgctcgatc tgtatgccca gccgttcctc
     3241 gcgagcggcg attactccgg gttcaaggaa tacgatgcgc cgcggtcgct cgcccggagt
     3301 gagtacgggc gtgatcgggg caccatcgcg ttttccacag ccggcgggcc gcattacacc
     3361 atcgaccccg atggcgcggg ccccgcaccg agcttcgtga tcgccgaccc cgacttcacg
     3421 cggtattcgt ggcggagcaa cgccgtggtg cggtgggagt atcggccggg atgcgtgctc
     3481 tacctgatct ggacccaggc acggtcgggg agcgcgccca ccggggacat ccagctgccc
     3541 cgtgacgcga atgcgctctt tcgcagcccc gcggacaaca tcctcctcat caaggcctca
     3601 tactggttcg cccggtgaag gcgccggggg ccttgtccac acctgcaagg agcagaccca
     3661 tgcgccactg gaagatgctg tcgattctct cgctcgtcgc ggtcacgtcc gccgcggccg
     3721 gaccgcggtc gccgacggtc gtggccgtcg cgatggtgcg ggacgcgacg ggggcgacct
     3781 acttcgcgcc gagcaacatc acggcccagc cgggagacac cttgcggttc atcgtgaccg
     3841 caggaatgca cgacgtcgat ttcgccgccg actcgaaccc cgcgggcgtg cggcttccgg
     3901 ccgcgacggc gctgatcgag catccggggg aggcagtgaa cgtcgtggtg gcgctgcctc
     3961 ctgggacata ctacttccag tgcgatcccc acgtcatgcg cggaatgacc gggcacctga
     4021 ccgtcgaagc gccggggcga tagccggtct ggtcggggcg gatccggcgg acgccccacc
     4081 ccgtgcacag ggtcgtccct tcccggccgc ggggctcgtt ggccgggttt cggggaggag
     4141 gatcagcgcc ctcgatcacg tcagccgccc gacgtcacgg tgggccgggc gcctcttcgc
     4201 agtccacggt tgacagacgc ccaggggctc aggacattcg ctgagcgcct gggcgtctct
     4261 gataaggagg gtcggtgcat ctcccgtccg tgtcactccg tcggtcgtgg ccgctgctgc
     4321 ccctgttgct cgtcgggtgc gtcaccgcgc ggctcaccga cgtgtggaag gatcccggcc
     4381 tgcccgcggc gcctctcacc catctgatgg tcgtgagcct ggcccccagc gaggtgctgc
     4441 gccggacgtg ggaagatgcg atggtggcgg ggctccaggg acacggtgtc gaggccacgc
     4501 cgtcgtacga cgtgtggccg gacggcccgc ccgagatcga ttcgctggcg gcggaggtgc
     4561 agcgcgaggc gttcgacggc gtcgtcctga cccacccggc cgacgtgcag accacccgga
     4621 cctacgtgca cggccagacg acgctcgtcc cgaccaaggt gtacgacaag tggaagcagc
     4681 actacgtgac gtactaccgc cgggtcgaga cgccgggcca cgtggaggtg catacgtcac
     4741 tgctcgtcga taccgacatc tggatggccg cggaccagtt ccggatggtg tggaccggca
     4801 ccacgcgggc catgaacccc acgtccaccg acgcgaccaa ccaggcgatc atccacgcca
     4861 tcctgccgcg ccttgccagg cagggagtga tcccgccggc gcggaagcag taggccggcg
     4921 ctagcgcctg atctcccgca ccgcctcggc cgccgcccgc cggaccgctg cgatcagccc
     4981 ggcgacgtga tcgacgcccc ccgcttcggc caggaacggg tccatcacgg tcgtgcgcag
     5041 caccaccagc ccctcggccc cggccgcccg ccagtcgtcc accgtgcaca caccgagctc
     5101 gcccagcacc gcgtcgaccg cgccctcgta catcggcgcc tgcagccggg tgcgcgtgag
     5161 catgtagtcg ggcgccgggc gcccgggctg gatgctcagg cgctcgtaga tcccctcgtt
     5221 cagcgcattg agttccacca gcgagccgat gtcgggatgg gtcagcgcaa agcagacgat
     5281 gctcaggtcg gggcgcggga gcgtgacgac ccggaagggc gccgctgctg ccggcgcggt
     5341 gagtgcggag tggagccggt gggcccccac gaccgtccgg gaaatgaggt gcccgtatcc
     5401 ccgctcatcg agcggcagga cccggtggct gagccacacg gccgcggccg cggccccagg
     5461 cttcgacccc tcgaggatgc agcgcccgag gaagagttcg tcgggatccc ggccggtcgc
     5521 ggtcaggtaa ggcgggtcga ccgccacgag ctcccgggcg cggcggtcct tgagcaggaa
     5581 cgcgcccgcg gggtagggaa cgaagcccag cttgtgcgga tccacgctga tcgagtcggc
     5641 ccggccgagc gcggtgatgg accgtaccca ctcctccgac ggccagtcga tcccggtcga
     5701 tgtccggatc tcctccgcgc tccgccgccg gccgtcggcc ccccaggtga tggccgaggc
     5761 gtaaccgccg aagcacgcat cggcgtggac gtggaacgcc aggcccagtt cgcggcgggc
     5821 ccgctcgcgg acctcgagca cctggtcgag gcggtcgacg gcgccctctt cggtcgtgcc
     5881 gcagacgctg acgcaggcga ggatcgccgt gcgcgagtcc gcgcagcggc ggacccgctt
     5941 ccacagggcg tcgggatcca tccggaaggt gcggtcgacc gggaccagcc tgagctggtg
     6001 ggccccgatg ccgagggcgc gcgcgatctt ttcccaggag tagtgcgccg tcgccgccac
     6061 caggacgacg gcggcgggga gctggtcgcc gtagtggcgg gccagccgca ggctgtagtc
     6121 ctggtaaccg atgctggcga gggagtaggc gtcgaccgcc gtgcgggcgg cctccgccgg
     6181 cacctgcgcc cggagcgcgt cccagaggtc gagcgccgcg ccgccgtcga tgttgaggag
     6241 cgaccagagc gggagatcgg cgagcgcggc acggcgtcca tccgggagcg tgacctcgag
     6301 cgagagcccc aggtccgccg ccgcgccggc gagcgccacg gggagataat ggagactccg
     6361 cgcgatccac agcgcctcga agttggccac cgtcccgccg gaggagaggt gcccccaggc
     6421 ggccttcggg tcgtagccca tcatcgccgc gagctgggcc gccacctcga gctcgaggcg
     6481 ggtggtgacc ggtgccgcct cccccgccac gttgttcggg ttgtagagca tcgtggcgat
     6541 gtacgccacc tgggcagcga tcgtctgctc gcccaccatg tgcgcctggt accggccgct
     6601 gaagaacggc acgtcctgct tgaggtcggc aaggaggccg aggagctcct gcgtgagcgt
     6661 cgcgacggag gcctcgtacc cttcccgccg ccggtccgcc tcccggatgc ggaggccgtc
     6721 ctcggggtga tggttgcggc gccagaacac gtggtcgcgg agcgcctcca ccatcaggcg
     6781 ctcgaggagg tcggcgttct ccgcccgggg ccccaggaac caggcccgca gggcctcatc
     6841 agctgagtga tccatgaacc ggagtttaag gtcggactgt gaggaaggcg tgaagcgggg
     6901 tgcaacgggg cttcatgcgt gcgtgcgggg gcttagcgct tagggcttag ggcatagggc
     6961 gtgacatacc cccgaggatc tgcaaggtgg gcgtgtcgat cggtccactc ccgggacgcc
     7021 aagccctaag ccctcagccc tcagctccaa gctctaagct ccaagctcta agctctaagc
     7081 tccaactctt aggtccatct ggcttcccgc cgtcacgtcc cgttcccaac gcgccatcac
     7141 gcacgacgcc aggcagttgc cgacgaggtt gaccgacgtc cgcgccatgt ccatcagcgc
     7201 atcgacgccc aggatcaccg ccacaccctc gagcgggagg tggaaggtcg cgagcgcgcc
     7261 cgcgaggatg acgagcgacg cccgcggcac ggcggccacc cctttgctcg tgagcatcag
     7321 cgtgagcatc atgagcacct gctggcccac cgtcagcggg atcccggcgg cctgcgccac
     7381 gaacaccgac gccaccgcga ggtagagggt ggagccgtcg aggttgaagg tgtagccggt
     7441 gggcaggacg aacgcgacga tgcgcgccgg cacaccgaac ttctcgaggt tctccatggc
     7501 cagcgggagt gccgcctcgc tcgaggcggt cgagaaggcg aggagccacg gcgccttgac
     7561 ccactgccag aagccgcgga tcggcacccg ggccgcgagc gcgatgggca agagcgccag
     7621 cagcacgaag gccaggagcg ccccgtacag cgtggcgacc aggaccagca ggttcttgag
     7681 cacgccgatc ccgctgttcc ccaccgtcac cgcgatggcg gcgccgatcc cgaacggcgc
     7741 gaacttcatc accagcgcgg tgaacttgaa catcacctcg gtgagcgcgt cacagaaggc
     7801 gagcatcgtc tccttccgct tgccggtcag ctgcgccagc ccgatcccga acagcaccgc
     7861 gaagaagacc acctgcagca cctcgttctt cgccgcggcg tcgaagaagc tggtggggac
     7921 ggtatgctca agcacgccgc tcagcgagag cgtggtctcc gccagctgct gcccttcctg
     7981 ggccgaggcg gcggccagat tcacgccgtc gccggggcgg atgacgttga ccgccaggag
     8041 cccgatcacg agcgccacgg tggtcaccgc ctcgaagtag aagatggact tgaccgcgag
     8101 gcggccgacc tgcttcaagt cgtccccgtg cccggcgatc ccgaccacga gcgccgagaa
     8161 cacgagcggc accacgatgg acttgatcat ccggaggaag atctgtgcca ccgggttgag
     8221 ccagtggccg aggtccacgc cggtccagag cgcatggtcg gcccagccga tcaggatgcc
     8281 gaccaccatc gatatgagga tccactgcga gagcgtgacg gaccgcaggc gtgcgagcat
     8341 gagggggacc gttggagggg ggccggcggc cgccggcccg gcgatcagcg ggcggagagc
     8401 gcgctcgcct tggcgtcgat gccggcgagc agggacgcgt acgaggcggc gaacttggcc
     8461 accc
//
LOCUS       DASUAP010000379         6103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_217763, whole genome shotgun sequence.
ACCESSION   DASUAP010000379 DASUAP010000000
VERSION     DASUAP010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217763"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /gene="dusB"
                     /locus_tag="VFU45_10195"
     CDS             complement(<1..796)
                     /gene="dusB"
                     /locus_tag="VFU45_10195"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU5359481.1"
                     /translation="MKLPYPARSAVPLLLAPMAGVSEAPFRQICRRMGADVVLSEFLS
                     SEAIRRRIRNTLEGAEFEECERPIGIQIYGADPSAMAEATALVTEAYRPDFVDINFGC
                     PVKKVVQRNGGSGCLRDLDLVDRIIRACVAATHLPVTVKTRSGWSEECRDPVGIALRM
                     QDAGARAFTLHARTRTQMFGGAANWDEIARVKEALSIPVIGNGDVHTAEDALRMHRHT
                     GCDAIMVGRGAFGNPWLFRDAQALLAGEPRPPAPDVGERFGVALEHA"
     gene            878..1330
                     /locus_tag="VFU45_10200"
     CDS             878..1330
                     /locus_tag="VFU45_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5359482.1"
                     /translation="MHLREFFSDDAINLSIQGSGKDEVLAELVALLKVDDRSADALLK
                     LLRRRESLGSTGVGRGIAIPHCRTPVVARLHLAFGRHAAGIDYRAIDDRPVHYFFLIV
                     APPIEVSNQYLPVLGRIAQFAKEPDVPDRLAKVTGPEEFFALLAAKGV"
     gene            complement(1488..3062)
                     /gene="guaA"
                     /locus_tag="VFU45_10205"
     CDS             complement(1488..3062)
                     /gene="guaA"
                     /locus_tag="VFU45_10205"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU5359483.1"
                     /translation="MTHDPRPATQMDRILIIDYGSQFTQLIARRVREAHVYCEIHPPT
                     RSVAWIREWAPKGIIWSGGPNSVYDPGAPSADPGQFDLGVPILGLCYGFHLMAHLAGG
                     KVLPSNEKEYGRAMVKPSGGRMWQGFTAGEPTQVWMSHGDHIDAPPPGFVVTATSDNG
                     LIAAIEHEAKPFFALQFHPEVAHTLRGSELLENFLFSACHARPEWTAAHFIESEVARI
                     KALVPDGTRVICGLSGGVDSSVAAALVHRAVGDRLTCIFVDHGLLRLNERHQVESTFR
                     EHLGIDLRVVDASERFLGKLAGVSDPERKRKIIGHEFIEVFNDEARHVGSDVGFLVQG
                     TLYPDVIESVSPTGGPSVTIKTHHNVGGLPEKLPFKLIEPLRELFKDEVRQVGRDLGL
                     PEVMVGRHPFPGPGLAIRILGEVTREQCDILRKADAIYIEEIRAAGLYDDIWQAFAVL
                     LPIRSVGVVGDFRSYDQVVGLRAVTSRDGMTADWFPFPPDVMARISTRISNEVRGVNR
                     VVYDVSSKPPATIEWE"
     gene            complement(3173..4525)
                     /gene="lysA"
                     /locus_tag="VFU45_10210"
     CDS             complement(3173..4525)
                     /gene="lysA"
                     /locus_tag="VFU45_10210"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU5359484.1"
                     /translation="MGQGVLNQARARRTAPEARARLEEEAGLARVGGELHVGGVPLSA
                     IADAAGTPTFVYHADVIRQRYRALDAALAGLPHRICFAVKANSSLAVLALLEQLGAGA
                     DIVSGGELQRALRAGFPAGRIVFSGVGKTDEELDLAVRSGVGHIHLESVAELEALGRI
                     TERQGRRATVGIRVNPDVTADTHPFIATGQGGIKFGLPADQVAPAIDRIRRSPYLRLT
                     CLAMHIGSQLREPAPYAEGLSKLVALVEKARRAGVTTLDAIDLGGGLGIRYGAEPGMD
                     VVRFAETLRPFLGPLGLTVYLEPGRYLVGPAGVLLTEVLYRKHSGGRDFVVVDAAMND
                     LLRPSLYQAYHGIVEVEAKGRPVAPVDVVGPVCETGDFLAVGRPLPGVEPGERLAVLG
                     AGAYGFVMSSNYNTRARAAEVLVSDGRWAVVRPRESVDELFKTEAVDPFTPVTVGKPR
                     "
     gene            complement(4507..5955)
                     /gene="proS"
                     /locus_tag="VFU45_10215"
     CDS             complement(4507..5955)
                     /gene="proS"
                     /locus_tag="VFU45_10215"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU5359485.1"
                     /translation="MSQEKAVTPRAEDFSAWYNDVIAKAELADYSPVRGCMVIRPNGY
                     AIWEQMQHALDGLFKETGHQNAYFPLFIPQSFLAREAEHVEGFAPETAVVTHGGGKEL
                     EEPLVVRPTSETIIYAMYAKWIQSYRDLPLLINQWANVVRWEMRTRLFLRTTEFLWQE
                     GHTAHATEAEAEAETLMILRLYRHFMEEWMAMPVLTGRKTDSERFAGALRTYACEGLM
                     QDNRALQAGTSHNLGQNFAKAFDVTFQTADGRLDHVWNTSWGVSTRLIGGLIMTHSDD
                     AGLICPPRLAQWQVVIVPIYRSDDERAAVMAAADSLLKELRADGIRTHLDARDGMKPG
                     AKYYEWEARGVPLRLELGPRDVAAGSAMLARRTGGKKEPVSLVGIGKRLASEMSAMQE
                     ALLTAARARREAASVRGATREEFIARMEGEGGFVYAGFCGDGACEAEIKEQTKATIRV
                     LPDEEFRSPRAPTVCMWCGRPSVAEAVWAKAY"
BASE COUNT          910 a         2218 c         2039 g          936 t
ORIGIN      
        1 gtgcgtgctc gagcgccacg ccgaagcgct cgccgacatc gggcgccggg gggcggggct
       61 cccccgccag gagcgcctgc gcgtcgcgga agagccacgg gttcccgaac gcgccgcgcc
      121 cgaccatgat ggcgtcgcac ccggtgtgcc ggtgcatccg gagcgcgtcc tcggcggtgt
      181 ggacgtcgcc gttgccgatc accgggatcg agagcgcctc cttgacccgg gcgatctcgt
      241 cccagttggc ggcgccgccg aacatctggg tgcgggtacg ggcgtggagg gtgaaggccc
      301 gggcccccgc gtcctgcatc cggagcgcga tccccaccgg gtcccggcac tcctcgctcc
      361 agccgctcct ggtcttgacg gtcaccggca ggtgggtcgc ggccacgcag gcgcggatga
      421 tccggtcgac caggtcgagg tcgcgcaggc agccggagcc gccgttgcgc tggacgacct
      481 tcttgaccgg acagccgaag ttgatgtcca cgaagtcggg gcgatacgct tcggtgacga
      541 gcgccgtggc ctcggccatt gcggaggggt cggcgccgta gatctggatc ccgatcggcc
      601 gttcgcactc ctcgaactcg gcgccttcga gggtgttccg gatgcggcgg cggatcgcct
      661 ccgacgacag gaattccgac agcaccacgt ccgcccccat ccgccgacag atctggcgaa
      721 agggcgcctc tgagaccccc gccatcgggg ccaggaggag cggcacggcg ctccgggcag
      781 gatagggcaa cttcatatgc ggaaaatact tgcccgcctg cggcccggca acgcggcgcg
      841 ttgacgccgg ccctcgcccg cggttagctt gcgcgccatg cacctgcgcg agttcttctc
      901 cgatgacgcc atcaacctct ccatccaggg ctccggcaag gatgaggtcc tggccgaact
      961 ggtggcgctc ctcaaggtcg acgaccgctc cgccgacgcg ctgctcaagc tgcttcggcg
     1021 ccgcgagtcc ctcggctcca ctggcgtcgg ccgcggtatc gcgatcccgc actgccgcac
     1081 ccccgtcgtc gcccggctcc acctcgcgtt cggacgccac gccgcgggga tcgactaccg
     1141 cgccatcgac gaccggccgg tgcactactt cttcctgatc gtggcgccgc cgatcgaggt
     1201 gtcgaaccag taccttcccg tgctggggcg gatcgcccag ttcgccaagg aacccgacgt
     1261 gccggaccgc ctggccaagg tcacgggccc tgaggaattc ttcgcgctcc tggcggccaa
     1321 gggcgtgtga gtccggtgag cggtgaacgg taagcggtga gccggtacgg accggtcacc
     1381 ccgcatgaca acggccctcc gatgatcgac gggatcggag ggccgcttct tttgatggtc
     1441 ccacctgacg tctgctcacc ccttaccgct gaccgggcac cgcgcaatca ctcccactcg
     1501 atcgtggccg ggggcttcga ggacacgtcg tacaccaccc ggttgacgcc ccgcacctcg
     1561 ttggagatcc gcgtgctgat ccgggccatc acgtccggcg ggaaggggaa ccagtcggcg
     1621 gtcatcccgt cgcggctggt cacggcccgg agccccacga cctggtcgta gctgcggaaa
     1681 tcgcccacca ccccgacgga gcggatgggg agcagcaccg cgaacgcctg ccagatgtcg
     1741 tcgtacagcc ccgccgcccg gatctcctcg atgtagatgg cgtcagcctt ccgaaggatg
     1801 tcacactgtt cccgggtgac ctcgcccagg atccggatgg ccaggccggg ccccgggaac
     1861 ggatggcggc ccaccatcac ttcgggaagg ccgaggtcgc gccccacctg ccgcacctcg
     1921 tccttgaaga gctcacggag cggttcgatc agcttgaacg ggagcttctc cggcaggcca
     1981 cccacgttgt ggtgggtctt gatggtcacg gaggggccgc cggtggggct caccgactcg
     2041 atcacgtcgg gatacagggt cccctgcacc aggaacccga cgtcggaccc gacatggcgg
     2101 gcctcgtcgt tgaagacctc gatgaactcg tggccgatga tcttccgctt ccgctcgggg
     2161 tcgctcacgc cggcgagctt ccccaggaaa cgctccgagg cgtccaccac ccggaggtcg
     2221 atgccgaggt gttcgcggaa cgtggactcc acctggtgcc gctcgttgag ccggagcagg
     2281 ccgtggtcga cgaagatgca ggtgaggcgg tcgcccacgg cccggtgcac cagcgccgcc
     2341 gcgaccgagc tgtcgacgcc accggagagc ccgcagatga cgcgggtgcc atccgggacc
     2401 agcgccttga tcctcgccac ttcggactcg atgaagtggg cggcggtcca ttccggccgg
     2461 gcgtgacagg ccgagaagag gaagttctcg agcagctcgc tcccgcggag cgtgtgcgcc
     2521 acctcggggt ggaactgcag cgcaaagaac ggctttgctt cgtgttcgat ggcggcgatg
     2581 agcccgttat cgctggtggc ggtgacgacg aagcccggcg gcggggcgtc gatgtggtcg
     2641 ccgtggctca tccagacctg ggtcggttcc cccgcggtga acccctgcca catccttccg
     2701 cccgagggct tcaccatcgc ccgcccatac tccttctcat tcgaggggag caccttcccg
     2761 ccggcgaggt gggccatcag gtggaatccg tagcagagcc cgaggatcgg cacacccagg
     2821 tcgaactgcc ccggatcggc cgaaggggcc cccgggtcat agaccgagtt cggcccgccg
     2881 gaccagatga tccccttcgg cgcccactcc ctgatccacg ccaccgagcg ggtgggcggg
     2941 tggatctcac aatagacgtg cgcctcgcgc acccgccgcg cgatgagctg ggtgaactgg
     3001 ctgccgtagt cgatgatgag gatgcggtcc atctgcgtcg cgggtcgtgg gtcgtgggtc
     3061 atgggactct gatcgcaggg cgcacggaca ggtgaaggga ctggcgaacg atcggaagaa
     3121 cccctcgaac agcgggccat ccacccgcga cccgcgactc acgacccatg actcacctcg
     3181 gctttcccac cgtcaccggc gtgaacgggt ccaccgcttc ggtcttgaac agctcgtcga
     3241 ccgactcgcg aggccgcacc actgcccagc gtccgtcgga caccagcact tcggcggcgc
     3301 gggcgcgggt gttgtagttg ctgctcatca cgaagccgta cgcgcccgcg cccaggacgg
     3361 ccaggcgctc ccccggctcg acgcccggga gcggccggcc gactgccagg aagtcaccgg
     3421 tctcgcagac cggccccacc acgtcgaccg gggccaccgg gcgtcccttc gcctcgacct
     3481 ccacgatccc gtggtacgcc tggtagaggc tcggacggag caggtcgttc atggcggcgt
     3541 cgacgaccac gaagtcccgg ccccccgagt gcttccggta gagcacctcg gtgaggagga
     3601 cgccggcggg gcccaccagg tagcgccccg gctcgaggta gacggtgagg ccgagcggtc
     3661 cgaggaaggg gcgcagcgtc tccgcgaatc gtaccacgtc catgccgggc tccgccccgt
     3721 agcggatgcc gagcccgccg ccgaggtcga tcgcgtcgag ggtggtgacc cccgcgcggc
     3781 gggccttttc taccagcgcc accagcttgc tcaagccctc cgcatagggc gcaggctccc
     3841 gcagctggga gccgatgtgc atcgccaggc aggtgagccg gaggtagggc gaacggcgga
     3901 tccggtcgat ggctggcgcc acctggtccg ccggcagccc gaacttgatc ccgccctgcc
     3961 ccgtcgcgat gaacgggtgg gtatccgcgg tgacatcggg attgacccgg atgccgacgg
     4021 tggcgcgccg cccctggcgt tccgtgatcc gccccagtgc ctcgagttcg gcgaccgact
     4081 ccaggtggat gtggcccacg ccggagcgca cggccagatc cagttcctca tccgtcttcc
     4141 cgaccccgct gaagacgatc cgcccggcag ggaagccggc ccgcagggct cgctgcagct
     4201 caccgccgga gacgatgtcc gcaccggcgc ccagctgttc gagcagggcc agcacggcca
     4261 ggctggagtt tgccttgacg gcgaagcaga tccggtgcgg caggccggcc agcgccgcat
     4321 cgagcgcgcg gtagcgctgg cggatgacat cggcgtggta gacgaaggtc ggcgtcccgg
     4381 cagcgtcggc gatggcggag agcgggaccc cgccgacatg aagctcgccg ccgacgcggg
     4441 ccaggcccgc ttcctcctcc agacgtgccc gcgcttccgg tgcggtgcgc cgggcccggg
     4501 cctggttcag tacgccttgg cccacaccgc ctccgccacg ctggggcgtc cacaccacat
     4561 gcagaccgtc ggcgccctgg gcgagcgaaa ttcctcgtcc ggcagcaccc ggatggtggc
     4621 cttggtctgc tccttgatct ccgcctcgca ggcgccatca ccgcagaacc ccgcgtacac
     4681 aaagccccct tcaccctcca tccgcgcaat gaactcctcg cgcgtggcgc cccgcacgct
     4741 ggccgcctcg cgccgcgcgc gggcggccgt gagcagcgcc tcctgcatcg cggacatctc
     4801 gctggcgagc cgcttgccga tcccgaccag cgataccggc tccttcttgc cgccggtgcg
     4861 ccgcgcgagc atggccgagc cggccgcgac atcgcgcggg ccgagttcca gccgcagcgg
     4921 cacgccccgg gcttcccact cgtagtattt ggcgcccggc ttcatgccgt cgcgggcatc
     4981 gagatgggtc cggatgccat cggcacggag ctccttcagc aggctgtccg ctgccgccat
     5041 caccgccgcc cgctcatcgt cggaacggta gatcgggacg atcaccacct gccactgcgc
     5101 caggcgcggc gggcagatga ggccggcgtc gtcgctgtgg gtcatgatca gcccgccgat
     5161 gagccgggtg gacacgcccc acgacgtgtt ccacacatga tccagccggc catcggccgt
     5221 ctggaaggtc acgtcgaacg ccttggcgaa gttctggccc agattgtggc tggtcccggc
     5281 ctgcagcgcc cgattgtcct gcatcagtcc ctcgcaggcg taggtgcgca gcgcgccggc
     5341 gaaccgctcg ctgtcggtct tccggccggt cagcaccggc atcgccatcc actcctccat
     5401 gaagtggcga tagagccgca ggatcatgag cgtctccgcc tcggcctcgg cctcggtggc
     5461 atgcgcggtg tggccctcct gccagagaaa ttcggtggtc cggagaaaga ggcgcgtccg
     5521 catctcccag cgcaccacgt tggcccactg gttgatcagg agcggcaggt cgcgatagct
     5581 ctggatccac ttcgcgtaca tcgcgtagat gatcgtctcc gacgtgggcc gcaccaccag
     5641 cggctcctcc agctccttgc cgccgccgtg cgtgaccacc gccgtctccg gcgcgaatcc
     5701 ctcgacgtgc tcggcctcgc gcgcgaggaa gctctgcggg atgaaaagcg ggaaataggc
     5761 gttctggtgg ccggtctcct tgaagagccc gtccagggca tgctgcatct gctcccagat
     5821 ggcgtagccg ttggggcgga tcaccatgca gccgcgcacc gggctgtagt ccgccagctc
     5881 cgccttcgcg atcacgtcat tgtaccacgc gctgaaatcc tcggcgcgag gcgtcaccgc
     5941 cttctcctgc gacatcccgt ctccttgacg atccggtgcc ggcgctcagc tgcgcccgca
     6001 cgcctcggcc aagcgcgcca ccacggcatc gcgcggcacc tgctcctggc caccacccct
     6061 caggtccttg agccccacca tgccgagcgc ccggtcatcg ggc
//
LOCUS       DASUAP010000380         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_872153, whole genome shotgun sequence.
ACCESSION   DASUAP010000380 DASUAP010000000
VERSION     DASUAP010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872153"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFU45_10220"
     CDS             complement(<1..717)
                     /locus_tag="VFU45_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359486.1"
                     /translation="MYRVSSSGLIYAVIVGAWAAYLVPMWLRRQDELNEARPTERFST
                     AIRLLSGRAAMERRVAKARGESVEPDADIAAEDDIDLAGAVDVRNLAVPVTEVRHPLT
                     RPAHAPAATPATGSAKGNGTPKTPVPSPRAKVLARRRRTTTLLFLAFTIGAIAAAVGG
                     VALLWAPAVPAVLLTLYIGQMRRQERRRYEVRLDQRRAAEAARRLRARSEAAAPASES
                     APRERERPATEPAATPPAASS"
     gene            complement(832..1422)
                     /locus_tag="VFU45_10225"
     CDS             complement(832..1422)
                     /locus_tag="VFU45_10225"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU5359487.1"
                     /translation="MDGDITLRPIRMRDQRAWREVNRRNREWLRPWEATVPPAPPGHV
                     AQRPTYRQMVRHLRREAGAGRMLPFVIEYQGRLAGQLTVAGITWGSMCSAHIGYWVDE
                     AVAGRGVMPTSVALAVDHCFRRVGLHRVEVCIRPENGPSRRVVEKLGFREEGLRPRYL
                     HIDGGWRDHLVYALTAEEVPEGLVHRWHQARAQRTT"
     gene            complement(1446..2048)
                     /locus_tag="VFU45_10230"
     CDS             complement(1446..2048)
                     /locus_tag="VFU45_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEU5359488.1"
                     /translation="MRALAVTASNRAAAGVYEDRGGPLIVAALRELGFEVDGPRVVPD
                     GDPVGQVLREAVEAAYDLVVTTGGTGISPTDRTPEVTRAVLDYEVPGIAEAIRAAGRH
                     KVATAVLSRGVAGVSGRTLVVNLPGSTGGVKDGLAVLAPLVVHAIDQIRGGDHPNEEA
                     GDPLVDHPHNHPEERADDRTTDHPTKDRRDHPNSVTGRPS"
     gene            complement(2066..>2459)
                     /gene="moaC"
                     /locus_tag="VFU45_10235"
     CDS             complement(2066..>2459)
                     /gene="moaC"
                     /locus_tag="VFU45_10235"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU5359489.1"
                     /translation="TARATGRVLVAPRVVELLRGAGVPKGDALATARIAGIMGAKRTP
                     ELIPLCHPLALSGVTVDLAVADDAVEIAATVKTTDRTGVEMEALTAVSVAALAVVDMV
                     KAVDKGAVITDVRVEAKTGGKSGDWRRS"
BASE COUNT          351 a          872 c          858 g          378 t
ORIGIN      
        1 ggaactcgca gcagggggtg ttgcggcagg ctcggtcgcc gggcgctcac gctccctggg
       61 tgcgctctcc gaagcgggcg ccgccgcctc ggagcgggcg cgtagccggc gcgcggcctc
      121 tgccgcgcgc cgctgatcga gccggacctc gtaacgacgc cgctcttgcc gtcgcatctg
      181 gccgatgtaa agggtgagca gaaccgcggg gacggcaggc gcccacagca gcgcgacccc
      241 gccgacggcc gcggcaatgg cgccgatggt gaaggcgagg aagagcagcg tagtggtacg
      301 gcggcggcgc gcgaggacct tggcgcgcgg gctgggcacg ggcgttttcg gggtcccgtt
      361 ccctttcgcg ctccccgtcg cgggagtcgc cgccggagca tgagcggggc gtgtgagggg
      421 gtgccgcacc tcggtcacgg ggacggcaag gttgcggacg tccacggccc cggccaggtc
      481 gatgtcgtcc tcggctgcga tgtcggcatc gggctcgacg ctctcacctc gcgctttcgc
      541 gacacgccgc tccatcgccg cccgcccgga caggagccgg atggcggtgc tgaagcgttc
      601 cgtcggacgc gcttcgttga gctcgtcctg cctacggagc cacatcggca ccaagtaggc
      661 agcccaggcc ccgacgatga ctgcgtagat gaggccgcta ctgctcaccc ggtacacggt
      721 aggggtgatc gagacacgcc atacggaatt tggcccggtg tgtcgcacga tccggctgat
      781 atgccgagaa ttttgtgcga ccacgagccg catttcgaac aattgtttta ttcaggtggt
      841 gcgctgggca cgagcctggt gccagcggtg caccaggccc tccggcacct cctcggccgt
      901 cagcgcgtag accaggtggt cgcgccagcc gccgtcgata tgcagataac gcggccgcag
      961 tccttcttcg cggaagccga gtttttccac cacccggcgg ctggggccgt tctcggggcg
     1021 aatgcacacc tcgacgcggt gcaggcccac gcggcggaag cagtggtcga cggcgagtgc
     1081 gacggaagtc ggcatcaccc cgcggccggc caccgcctcg tcgacccagt acccgatgtg
     1141 ggcggagcac atcgagcccc aggtgattcc ggcaacggtg agttgacccg ccagccgccc
     1201 ctggtactcg atcacgaacg gcagcatgcg gccggcgccg gcctcccggc gcagatgccg
     1261 gaccatttgc cggtaagtgg ggcgctgcgc cacatgcccg ggcggtgcgg gaggcacggt
     1321 cgcctcccag gggcgcaacc actcgcggtt gcgacggttg acctcgcgcc aggcgcgctg
     1381 atcgcgcatc cggatcgggc ggagggtgat gtcaccgtcc accagctcag ccggccagga
     1441 cccgttcagc tcggtctccc cgtgacggaa ttcggatggt ccctccggtc cttcgtcgga
     1501 tgatcggtcg tgcgatcatc cgctcgctct tccgggtgat tgtgcgggtg gtcaaccagc
     1561 ggatcgcccg cttcttcgtt cgggtgatct cccccgcgga tctgatcgat ggcgtgcaca
     1621 accagaggcg cgagtacggc caggccgtcc ttcacccctc ctgtggagcc gggaaggttc
     1681 accacaagag tgcgtccact cacccccgcg acgccacggg acagcacagc ggtcgccacc
     1741 ttgtggcgcc ctgccgcgcg gatcgcctcc gcgatcccgg gcacctcgta atcgagcacc
     1801 gcgcgggtga cctccggggt ccggtcggtc ggcgagatgc cggtgccgcc ggtggtgacc
     1861 acgaggtcgt acgccgcctc gaccgcctcc cgcagcacct gcccgacggg gtcgccgtcg
     1921 ggcacgaccc ggggtccgtc aacctcgaac ccgagctccc gcagggcggc gacgatcagt
     1981 ggtccgcccc ggtcctcgta gactccggcg gcagcgcgat tggaggcggt gaccgccagc
     2041 gcccgcatcg tgcgcggccg gccgttcatg aacggcgcca gtcgccggac ttgccgccgg
     2101 tcttcgcctc gacccggaca tcggtgatga ccgcaccctt gtcgaccgcc ttcaccatgt
     2161 ccaccacggc gagcgcggcg acgctcaccg cggtcagcgc ctccatctcc actccggtgc
     2221 ggtcggtggt cttcacggtg gctgcgatct cgacggcgtc atcggccacc gccaagtcga
     2281 cggtgacgcc cgacagagcc agcggatggc acagcgggat cagttccggg gtgcgcttgg
     2341 cgcccatgat tcccgcgatg cgcgcggtgg ccaaggcatc gcctttgggg acgcccgcgc
     2401 cacggagcaa ctccacgacg cgtggtgcca cgaggacgcg tcccgtggca cgcgccgtg
//
LOCUS       DASUAP010000381         3497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_694866, whole genome shotgun sequence.
ACCESSION   DASUAP010000381 DASUAP010000000
VERSION     DASUAP010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694866"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1592
                     /locus_tag="VFU45_10240"
     CDS             195..1592
                     /locus_tag="VFU45_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEU5359490.1"
                     /translation="MATPLAAFSIRLRAKIPNQPGVLGQLATAIGAAGGSLVGIEIVA
                     TDGATVTRDVVVFCGSEGHAADVEKAARGVGGIEIVEVEDRTFAVHQAGKLSIESKFP
                     LHDRDDLAMAYTPGVARVCMAIAHEPALVHRYTMKASTVAVVTDGTAVLGLGDIGPEA
                     ALPVMEGKAVLFKEFGGVDAVPVCLKVSSPDELVETVQRMEPMFGGINLEDIAAPHCF
                     EIEQRLIDTLSIPVFHDDQHGTAVVVLAALRNACRITGKDLSGLRVVLSGAGAAGVAI
                     TKILMTAGAKDIVAADRKGAIHKGREALDPSKQWLADNANPEGRSGSLQDILRGADVF
                     VGVSGPGLLNRGDVEAMADAPIVFALANPVPEILPEEAEGVVAVMATGRSDYPNQINN
                     VLCFPGIFRGALDAGATRITENMKLAAADAIASAVPEDKLRSDYIVPSVFVPGVGEAV
                     AAAVADAARADGVTR"
     gene            complement(1582..2067)
                     /locus_tag="VFU45_10245"
     CDS             complement(1582..2067)
                     /locus_tag="VFU45_10245"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5359491.1"
                     /translation="MQWQLREVASDFFETADYRCTTTTTLRASPDAVFYALAHDPSGW
                     GEWNPGFSSKGRYITPPPQGRGSVREVIMAGLRYTDTILQWEEPRHWAFCVSQAGAPF
                     ARALGEDYRITPDGDGSVVQWTIAMEPRLALRAMQPLMDAFLPRYFKRAMRNLDKKLS
                     G"
     gene            2345..2914
                     /locus_tag="VFU45_10250"
     CDS             2345..2914
                     /locus_tag="VFU45_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359492.1"
                     /translation="MPAGSSRWVAAVGFPSIGLVAAAQMGVPLERLALVPAPGERWAE
                     VVAALLDGVDLLLLSPPAGLRASDARRLAARARERGVVLVPVRHQGSGRWPESPEVRM
                     EADPVEWTGLGAGFGYLRHRRVEIAVEGRRVAGGRRRSAPFWMPGPEGVRPAEAGRPG
                     EAGLPAEVARPAEIAQAGAGPVLERAAAG"
     gene            2920..>3497
                     /locus_tag="VFU45_10255"
     CDS             2920..>3497
                     /locus_tag="VFU45_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359493.1"
                     /translation="MRALVAWFPDWPLVAAGCPPDIPALVLHANRVVAATPAARAEGV
                     REGLRRREAQARCPGVRVVEEDAGRDARAWEPAVAAVESLAPAVEVVASGELVLATRG
                     PSRYFGGDAALAELVCRAIDKAAGQEGCRGGVADGRFAAALAARAAGNPSRVLVVRRG
                     DSPRWLAPQPVTSLGAGYEELADLLVRLGIRTL"
BASE COUNT          477 a         1163 c         1320 g          537 t
ORIGIN      
        1 gagcgccatc ggaatgagaa aacgccgccg gcggcggcgc ttctggtgtc aaacgttcgg
       61 gcgaggcctg gtccgaccca tcccccggag cggccagcgg ccgacctcgc ccgaacgccc
      121 cctctcgacc ttcaatcgta gagtgccaag tggcacaccg gcaaggggtc cgactgcata
      181 gctatcctcc gaagatggcc acgccgttag cagcgttctc catccgtctc cgcgcgaaga
      241 tccccaacca gcctggcgta ctcgggcagc tcgccaccgc gatcggagca gccggcggga
      301 gcctcgtcgg catcgagata gtggcgaccg acggtgccac ggtcacccgc gacgtcgtcg
      361 tgttctgcgg atcggaaggc cacgcggccg acgtcgagaa ggccgcacgc ggggtggggg
      421 gcatcgagat cgtcgaggtc gaggaccgca ccttcgcggt gcaccaggcc gggaagctct
      481 cgatcgagag caagttcccc ctgcacgacc gggacgatct tgcgatggcg tacacgccag
      541 gcgtcgcccg ggtgtgcatg gcgatcgccc acgagcctgc tctcgtgcac cgctacacga
      601 tgaaggcgag caccgtggcc gtcgtcacgg acgggacggc cgttctcggc ctgggcgaca
      661 tcggccccga ggccgcgctg cccgtgatgg aaggcaaggc ggttctgttc aaggaattcg
      721 gcggcgtcga cgccgtcccg gtgtgcctga aggtctcgtc ccccgacgag ttggtggaga
      781 cggtccagcg gatggagccg atgttcggcg gcatcaacct cgaggacatt gcagcgccgc
      841 actgcttcga gatcgagcag cgcctcatcg acacactgtc gatacctgtc ttccacgacg
      901 accagcacgg caccgcggtt gtcgtgctcg cggctctacg caacgcctgc cggataaccg
      961 gcaaggacct ctccggcctg agagtcgtgc tgtccggcgc cggcgccgcc ggcgtggcca
     1021 tcaccaagat cctgatgacc gccggcgcca aggacatcgt cgccgccgac cgcaagggtg
     1081 cgatccacaa ggggcgcgag gcgctcgacc cgtcgaagca gtggctcgcg gacaacgcca
     1141 acccggaggg gcgttcagga tcgctgcagg acatcctgcg cggcgcggac gtcttcgtcg
     1201 gggtcagcgg gccgggtctg ctcaaccgcg gcgacgtcga ggccatggcc gacgccccga
     1261 tcgtgttcgc gctcgcgaac ccggtcccgg agatcctccc cgaagaggcg gagggagtgg
     1321 tggccgtcat ggcgaccggc cgcagcgact accccaacca gatcaacaac gtgctctgct
     1381 tcccgggaat cttccgcggc gccctcgatg cgggcgcgac gcgcatcacc gagaacatga
     1441 agctggccgc cgccgacgcg atcgcctcgg cggtcccaga ggacaagctc cgctccgact
     1501 acatcgtgcc gagcgtgttc gttcccggtg tgggcgaagc ggtcgcggct gcggtggccg
     1561 acgctgcccg cgccgacggc gtcacccgct gagcttcttg tcgaggttcc tcatggcccg
     1621 cttgaagtag cgcggcagga aggcgtccat cagaggctgc atggcgcgca aagcgagccg
     1681 tggctccatc gcgatggtcc actgcacgac ggacccgtcg ccgtcagggg tgatgcggta
     1741 gtcctcgccg agcgcccggg cgaacggcgc cccggcctgg gagacacaga acgcccagtg
     1801 cctgggctcc tcccactgga ggatcgtgtc ggtgtagcgc agtccggcca tgatcacctc
     1861 gcgcaccgaa cccctcccct gagggggtgg cgtgatgtag cggcccttgc tggagaatcc
     1921 agggttccac tcgccccacc cggacgggtc gtgagccagc gcgtagaaca cggcgtcggg
     1981 tgatgcccgc agggtggtgg tggtcgtgca gcggtagtcg gccgtctcga agaagtcgga
     2041 cgccacctcc cgcagctgcc attgcaccgc cggcaatgta gccgagccgt gggtggatcg
     2101 aacaaatgtt cgatacagtg ggggcatggt gaaggcagcg gtgcctatgg atgtccctat
     2161 ggatgtccct atggatgtca tagcggcgaa gggccgtccc gtcgccatgg cccgaacccg
     2221 gctgctggag gtggacggtg ctctgggggg tcttttcccg gcgggaggtt tgcagcgcgg
     2281 gagtgtcgtg gaggtggccg gcggggcggc gaggtcgttg tcgctggccc ttgcggcggg
     2341 ggtgatgcct gcagggtcct cccgctgggt tgctgcggtc ggtttcccgt cgatcggcct
     2401 ggtcgccgcc gctcagatgg gtgtgccgct cgagcgtctc gcgctggtgc cggcgcccgg
     2461 ggagcgatgg gcggaggtgg tggcggccct gctggacggg gtggacctgc tcctgctgtc
     2521 cccaccggcc ggcctgcgcg cctcggatgc ccgccggctg gcggcgcgcg cccgcgagcg
     2581 cggggtggtg ctggtcccgg tgcgccatca gggatccggc cgctggccgg agtccccgga
     2641 ggtgcgcatg gaggcggacc cggtggagtg gaccgggctc ggcgccggct tcggctactt
     2701 gcgccaccgg cgggtcgaga tcgctgtgga gggccgtcgg gtcgccgggg gtcgccggcg
     2761 ttcggcgccg ttctggatgc ccggccctga aggtgtgcga ccggcggaag ctgggcggcc
     2821 gggggaagca gggctgccgg cggaggtcgc gcggccggcg gagatcgccc aggcgggtgc
     2881 ggggccggtc ctggagcggg ccgccgcggg gtgacagctg tgcgcgcgct ggtcgcctgg
     2941 tttcccgact ggccgctggt cgccgccggc tgcccgcctg acatcccggc gttggtgctc
     3001 cacgccaacc gggtcgtcgc ggcgaccccg gcggcgcgcg ccgaaggggt gcgtgaaggg
     3061 ctgcgaaggc gcgaggcgca ggcgcgctgc cccggcgtcc gcgtcgtcga ggaggacgca
     3121 ggtcgtgacg cgcgcgcgtg ggagccggcg gtcgcggcgg tcgagtcgtt ggcgccagcg
     3181 gtcgaggtgg tcgcttcggg tgagctggtg ctcgcgacgc gcgggccttc ccggtacttc
     3241 ggcggggatg cggcactggc agagttggta tgccgcgcca tcgacaaggc ggcgggccag
     3301 gaaggctgcc gtgggggagt cgccgacggg cgcttcgctg ccgcgctggc cgcgcgcgct
     3361 gcgggcaatc cgtcgcgcgt gctggtcgtg cggcgtgggg acagcccgcg ctggctcgca
     3421 ccgcaacctg tcacttctct cggtgccggc tacgaggagc ttgccgatct gctggtgcgt
     3481 ctcgggatcc gcacact
//
LOCUS       DASUAP010000382         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_436021, whole genome shotgun sequence.
ACCESSION   DASUAP010000382 DASUAP010000000
VERSION     DASUAP010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436021"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2821
                     /locus_tag="VFU45_10260"
     CDS             <1..2821
                     /locus_tag="VFU45_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359494.1"
                     /translation="RSTDGGKTWTKTLFVDDTTGIQKITWAFDQPNVVFATTVKHFVE
                     PGPPATGAPRAPRGAEAKDAPSTTRIFKSTDEGITWQEIKGGGLPHLDGRTSMAVAMH
                     TSAQRVFLVGNFGLWRSDDGGTTWRQMDPDDKRVGNGQGGYNCGVYVSPSNPDVVYTI
                     NTSSYVSRDGGNTFTGFKGAPGGDDPQQMWIDPTNGQRIFLGMDQGATVSLDGGQTWS
                     TWYNQSTDQVYHISVDNSFPYWVYAPQQDAGAIRTRSRGNFGEITPLDWNPVGTWEWG
                     TLVADPLDPNIAYGSGSGIQKITYPSEQIIDVGPNTDPGAHLRTSFSMPIIWAPWDQH
                     ELLAGFQYVMATTDGGAHWRKLSPDLGYPKGVKPPPDSEADRPPRPNAPRGGAIESMS
                     ASTVGRGIIWVGTSNGLIKVTRDEGKTWADASIPGLPDSGFATITSVEASHHDAGTAY
                     AAVDLHRLGDYAPYVYRTRDFGKTWTKIINGLPTGQVSGSFVRVVRADTKKAGLLFAG
                     TESGMFVSFNDGDDWQSLQLNLPNTSFRDAVIKGNDLVVGTYGRGIWILDDISPLRQM
                     TPAIAGEAVHLFTPGDAVRLHRNVNQDTPFPPEVPHALNAPDGVIVYYYLGAKPTGDI
                     SLDVLDAAGNVVRHMSSAPITPVPEASHPPEPSFWIATPQPMPTALGTNRVNWDLRYD
                     APPAFSHSYGINANPGLTPPSPEGPLAAPGTYTFRLTVDGKAYTTTAKVANDPRSPAT
                     AMALAAQHALQLKIYRGANEAWDGFQQAATLKSALAALRATKPAAAVDSAAAAFDARI
                     DSVAGDTGGRGGRRFFGRGAKVPPTFVGVNGTMARQLDAQDLADMAPNPPMLAAYASA
                     CGDLKTVVTNWHGVVTHDLSAFNTLLQESHVATSAAPTTALAVPDCGPGVRAKGRTAA
                     ARVNAPDQNDDPDNPDAALGGDGGE"
BASE COUNT          508 a         1164 c          985 g          382 t
ORIGIN      
        1 ccgcagcacc gacggcggga agacctggac caagaccctc ttcgtcgacg acaccacggg
       61 catccagaag atcacctggg ccttcgacca gccgaacgtg gtctttgcca ccacggtcaa
      121 gcacttcgtc gagccggggc cgccggccac gggggcgccg cgggccccgc gcggcgccga
      181 ggccaaggat gcaccgagca ccacgcggat cttcaagtcg accgacgagg gcatcacctg
      241 gcaggagatc aaggggggcg gccttcccca cctcgacggc cgcacctcga tggcggtcgc
      301 gatgcacacc agcgcccagc gggtgttcct ggtgggcaac ttcggcctct ggcgctccga
      361 tgacggcggc accacctggc ggcagatgga tcccgatgac aagcgggtgg gcaacgggca
      421 gggcggctac aactgcggcg tgtacgtgag cccgtcgaac cccgacgtgg tctacaccat
      481 caacacgtcg agctacgtct cccgcgacgg cggcaacacc ttcaccggct tcaagggcgc
      541 ccccggcggc gacgacccgc agcagatgtg gatcgacccg accaacggcc agcgcatctt
      601 cctcggcatg gaccagggcg ccacggtgag cctcgacggc gggcagacct ggagcacctg
      661 gtacaaccag tcgaccgacc aggtgtacca catctcggtg gacaactcat tcccctactg
      721 ggtctacgcg ccgcagcagg atgccggcgc catccgcacc cggagccgcg gcaacttcgg
      781 cgagatcacc ccgctcgact ggaaccccgt cgggacctgg gagtggggca ccctcgtggc
      841 cgacccgctc gaccccaaca tcgcctacgg aagcggctcg gggatccaga agatcaccta
      901 cccgagcgag cagatcatcg acgtgggccc gaacacggac ccgggcgcac acctgcgcac
      961 ctccttctcg atgccgatca tctgggcccc ctgggaccag cacgagctcc tggccgggtt
     1021 ccagtacgtg atggccacca ccgacggcgg cgcccactgg cggaagctct cgcccgacct
     1081 cggctacccg aagggcgtca agccgccgcc ggacagcgag gccgaccgcc cgccgcgtcc
     1141 caacgccccg cggggcggcg ccatcgagtc gatgtcggcg tcgaccgtcg ggcgcgggat
     1201 catctgggtc ggcaccagca acggcctcat caaggtgacg cgggatgagg ggaagacctg
     1261 ggccgatgcc tcgatcccgg gcctccccga ctccggcttt gccaccatca cgtcggtcga
     1321 ggcctcccac cacgacgcgg gcaccgccta cgccgccgtc gacctccacc ggctcggcga
     1381 ctacgcgccg tacgtgtacc ggacgcggga cttcgggaag acctggacca agatcatcaa
     1441 cggcctcccc accggccagg tgagcggcag cttcgtccgc gtggtccggg ccgacaccaa
     1501 gaaggcaggg ctcctcttcg ccggcaccga gagcgggatg ttcgtctcgt tcaacgacgg
     1561 cgacgactgg cagtcgctcc agctcaacct ccccaacacc tcgttccgcg acgcggtcat
     1621 caaggggaac gacctcgtgg tcggcaccta cggccggggg atctggatcc tggacgacat
     1681 ctcgccgctc cggcagatga cccccgcaat agcgggcgag gcggtgcacc tcttcacgcc
     1741 cggcgacgcc gtccggctcc accggaacgt gaaccaggac accccgttcc cgcccgaagt
     1801 gccgcacgcg ctgaacgccc cggacggggt catcgtctac tactacctgg gcgccaagcc
     1861 gaccggcgac atctccctcg acgtgctcga tgcggcaggg aacgtggtgc gccacatgtc
     1921 gagcgcgccg atcacgccgg tgcccgaggc gtcacatccg ccggagccga gcttctggat
     1981 cgccacgccg cagccgatgc ccacggcgct cggcaccaac cgggtcaact gggacctgcg
     2041 ctacgacgcg cccccggcgt tcagccacag ctacggcatc aacgccaacc cggggctcac
     2101 gcccccctcg cccgagggcc cgctcgcggc gcccgggacc tacaccttcc gcctcacggt
     2161 cgacggcaag gcatatacca cgaccgccaa ggtcgccaac gatccccgct ccccggccac
     2221 cgcgatggcg ctggcggcgc agcacgcgct ccagctcaag atctaccgcg gcgccaacga
     2281 ggcgtgggac ggcttccagc aggccgcgac cctcaagtcc gcgctggcgg cgctccgcgc
     2341 caccaagccg gcggcggccg tcgattccgc cgccgcggcc ttcgacgcgc ggatcgactc
     2401 ggtggcgggt gacaccggcg gacgtggcgg acgccggttc ttcggccgcg gcgccaaggt
     2461 cccgccgacg ttcgtcgggg tcaacggcac gatggcccgc cagctcgacg cgcaggacct
     2521 ggccgacatg gcccccaacc cgccgatgct cgcggcgtac gcctccgcct gcggcgacct
     2581 gaagaccgtt gtcaccaact ggcacggcgt ggtgacccac gacctctcgg cgttcaacac
     2641 gctgctgcag gaatcccacg tggccacgag cgcggcgccg accacggcgc tggcggtgcc
     2701 cgactgcggg ccgggggtgc gggccaaggg acgcaccgcc gccgcgcggg tcaacgcgcc
     2761 tgaccagaac gacgaccccg acaaccccga cgccgcgctc ggcggcgacg gcggggaatg
     2821 aaccagcggt agtaccgccc cgccccgtgc ggggcgggtt tttcccttga cgcattccca
     2881 tatcggcata tattgggtcc atggcccggc tggcaacgac ctcggacgtg ttcaatgcgg
     2941 tggcggagcc ccgccgccgc cagatcctca ccctgctgtc gggtggcgag cggtcggtca
     3001 acgacctcgc gcggcagctc cgggtccggc agccgcagg
//
LOCUS       DASUAP010000383         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_762615, whole genome shotgun sequence.
ACCESSION   DASUAP010000383 DASUAP010000000
VERSION     DASUAP010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762615"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VFU45_10265"
     CDS             complement(<1..1310)
                     /locus_tag="VFU45_10265"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5359495.1"
                     /translation="MSRATILIADDHDTIRAAFRSFLEGHGYAVVEASSAAQAEQVFR
                     QAGPDAAILDHHLPDGDALGLLPVLRAGDPSVGIIIVTGHGTIDLAVQAIKAGADHFL
                     TKPADLPAALVLLERVLERRRERRRHAAEDAARSDDGAPDPFLGNGVVIRRLAETARR
                     LVAVDNPVLILGETGSGKGVLARWLHRNGPRAEESFVDLNCAGLSRELLESELFGHAK
                     GAFTGAATAKQGLLEIGHHGSVFLDEVGDLDPLVQPKLLKVVEDRTFRRLGEVQDRTV
                     DVRIIAATSLDLARLVREQRFRSDLYFRINTLTLTVPPLRERSDDLPLLARQLLRRIA
                     GRRGRREVELADDAMARLKAYPWPGNVRELQNVLERAVLLSDHTLLRAADLHFDAAPE
                     MARSVDGAFPTLAELERRHIAEALERTGGNVPEAAKLLDVPRSTL"
     gene            complement(1322..>3555)
                     /locus_tag="VFU45_10270"
     CDS             complement(1322..>3555)
                     /locus_tag="VFU45_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5359496.1"
                     /translation="VLAQVDTVAADAAAMDRAADSVLAELRAGHRHGAETLIAPLGRW
                     YLSSLDHLRKARSVASQAQEGLVADQERSTRNANVIVRALALLVLLACAVAFDAGLDA
                     DRRMRSDAEERELALQRLAAAETDLRLAQEKLTHATEERFRRLVTYAPEGIYTLDPSG
                     AITTANPALERLLALPAGSILGRNFREFVHPEDLPTSIEANRQRLLGNVPGNGAPVHR
                     RVLRSDGAYFEAEVNTGPLFDPDGAVVGILGIMRDVSERRNVERALAQSEERYRTLVD
                     YAPDPIYTLSLDGYVTSVNRACQVLTGHPASAMIGTHFSALLHPDDLARARDVNQETM
                     HGATGTKGAIRIRKADGAYVEVEVTSGPLGRSGTAGVLGILRDVTEVRRAAAEEAKLT
                     ALVRAAAQDWMLTFDTVNIGLVVLDASGTIHRLNEAARRLAGFPFSELIGRPLGWVGA
                     DDPWGPAAELAAATPEGVVGRSRVIDRGPERPAWSLTAVRYAAPDGERVILTIRDVSD
                     SFQLQRSVQRAETMAALGVLVAGVAHEVRNPLFAISATLDAFEARFGADEAYRKWAAP
                     LRPEVERLSQLMSDLLEYGRPPELQRVETDVGAVLTSASERTVALAASRRIRLSVQVA
                     AGVPRTLADPGRLLQVFQNLFDNAVRHAPDGSVVEGVLDLRSEPEGPRLRCEVLDRGP
                     GFDPADLQRVFEPFFSRRRGGTGLGLSIVQRLVEQHRGTITASNRDGGGGCVTVTIPV
                     TRD"
BASE COUNT          490 a         1301 c         1276 g          488 t
ORIGIN      
        1 agggtgctgc gggggacgtc gaggagcttc gccgcttccg gcacgttgcc gccggtgcgc
       61 tccagcgctt cggcgatgtg gcgccgctcg agctctgcca gcgtcgggaa cgcgccgtcg
      121 accgagcgcg ccatctccgg cgcggcgtcg aagtggaggt cggccgcgcg gagcagggtg
      181 tggtcgctca ggagcaccgc gcgttccagc acgttctgga gctcgcgcac attgccgggc
      241 cagggatatg ccttgagccg cgccattgcg tcatcggcca gctccacctc ccgccgcccc
      301 cggcgtcccg cgatgcgtcg gaggagctgc cgcgccagga ggggaaggtc atccgagcgc
      361 tcgcggagcg ggggcaccgt gagcgtcagg gtgttgatgc ggaagtacag gtcgctccgg
      421 aagcgctgct cgcggaccag gcgggccagg tcgagcgagg tggccgcgat gatccgcacg
      481 tcgacggtcc ggtcctggac ctcgcccagc cggcggaagg tccggtcctc gaccaccttc
      541 aggagcttgg gctggaccag cgggtcgagg tcgcccactt cgtcgagaaa caccgagccg
      601 tggtgcccga tctcgaggag cccctgcttg gcggtagcgg cgcctgtgaa ggcgcccttc
      661 gcgtggccga agagctccga ctccagcagt tcgcgcgaga gtcctgcaca gttgaggtcc
      721 acgaacgatt cctcggcgcg cggaccgttc cggtggagcc agcgcgcgag cacacccttg
      781 cccgaaccgg tctccccgag gatgagcacc ggattatcca ccgccaccag gcggcgggcc
      841 gtttcggcca ggcgccgaat caccacgccg ttgccgagga acgggtcggg ggcgccgtcg
      901 tccgatcggg ccgcgtcctc ggccgcgtgg cggcggcgct cccggcgccg ctccagcacc
      961 cgttccagca gcaccagcgc ggccggcagg tctgcgggct tggtcaggaa gtggtcggca
     1021 cccgccttga tggcctgcac ggcgaggtcg atggtgccgt gaccggtcac gatgatgatc
     1081 ccgaccgagg ggtcgccggc gcggaggacg ggcaggagtc ccagagcgtc gccatcggga
     1141 aggtggtggt cgaggatcgc cgcgtcgggc ccggcctggc ggaagacctg ctcggcctgg
     1201 gccgcgctcg acgcctcgac caccgcgtag ccgtgccctt cgaggaacga acggaacgcc
     1261 gcgcggatgg tgtcgtggtc gtcggcgatg aggatcgtgg cgcgggacac ggatgctccg
     1321 atcagtcgcg ggtgacgggg atcgtcaccg tgacgcatcc cccgcccccg tcgcggttgg
     1381 acgcagtgat ggtgccccgg tgctgctcga ccagccgctg gacgatcgag agcccgaggc
     1441 cggtgccgcc ccgccggcgg ctaaagaacg gctcgaatac ccgctgcagg tcggccgggt
     1501 cgaaccccgg cccgcggtcc agtacctcac aacggagccg cgggccctcc ggttccgacc
     1561 gcaggtcgag cacgccctcc accaccgagc cgtccggtgc gtggcggacg gcgttgtcga
     1621 agaggttctg gaacacctgc agcagccgcc ccgggtcggc cagggtcctc ggcacgccgg
     1681 cagccacctg gaccgagagc cggatgcggc gggacgccgc gagggccacg gtgcgctcgc
     1741 tcgcgctcgt gagcactgcg ccgacgtcgg tctccacccg ctgcagctcc ggcggccggc
     1801 cgtattcgag gaggtcgctc atcagctggc tcaggcgctc cacctcgggg cggagcggcg
     1861 cggcccactt ccggtacgcc tcgtccgcgc cgaaccgcgc ctcgaaggcg tccagcgtgg
     1921 cggagatggc aaagagcgga ttccgcacct cgtgcgccac cccggccacc aggacgccga
     1981 gagcggccat cgtctccgcc cgctggaccg aacgctgcag ctggaacgag tccgacacgt
     2041 cgcggatcgt caggatgacc cgctcgccgt cgggtgcggc gtaccgcacc gcggtgagcg
     2101 accacgccgg ccgctccggc ccgcggtcga tcacccgcga ccgccccacc accccttccg
     2161 gcgtcgcggc ggcgagttcc gccgccgggc cccacgggtc gtcggcgccc acccatccga
     2221 gcggccgccc gatcagctcg ctgaagggga accccgccaa ccggcgcgcc gcctcgttca
     2281 gccggtggat ggtgccggac gcgtcgagca ccacgagccc gatgttgacg gtgtcgaagg
     2341 tcagcatcca gtcctgcgcc gccgcccgga ccagggcggt gagcttggcc tcttccgcgg
     2401 cggcgcgccg cacctcggtc acgtcccgca ggatgccgag gaccccggcc gtacccgacc
     2461 gaccgagcgg gccggacgtc acctcgacct cgacgtacgc gccgtcggcc ttgcggatgc
     2521 ggatcgcgcc cttcgtcccc gtcgcgccgt gcatcgtctc ctggttcacg tcccgggccc
     2581 tggcaaggtc gtccgggtgg agcaacgccg agaagtgcgt cccgatcatc gcggaggcag
     2641 ggtgcccggt cagcacctgg caggcccggt tcaccgacgt cacatacccg tccagcgaga
     2701 gggtgtagat cgggtccggg gcgtagtcca ccagcgtgcg gtaccgttcc tcgctctgcg
     2761 cgagggcgcg ttccacgttc cggcgctcgc tcacgtcgcg catgatgccg aggatgccga
     2821 ccaccgcccc gtccgggtcg aaaagcgggc ccgtgttcac ttcggcctcg aaatacgcgc
     2881 cgtcggaccg cagcacccgg cggtgcaccg gggccccgtt gcccggaacg ttgccgagca
     2941 gccgctggcg gttggcctcg atggaggtcg gcaggtcctc cgggtgcacg aactcccgga
     3001 aattccggcc caggatcgac cccgccggga gggccaggag ccgctccagg gccgggttgg
     3061 cggtcgtgat cgcacctgac gggtcgagtg tgtagatccc ctcgggcgcg taggtcacga
     3121 gccggcggaa gcgctcttcc gtggcgtggg tgagcttctc ctgcgccagg cgcaggtcgg
     3181 tctcggccgc cgcaaggcgc tgcagcgcaa gctcacgctc ctccgcgtcc gaacgcatcc
     3241 tgcggtccgc atcgagcccc gcatcgaacg ccaccgcgca cgccagcagc acgagcagcg
     3301 ccagcgcgcg cacgatcacg ttcgcgttgc gggtggagcg ttcctggtcg gcgacgagcc
     3361 cttcctgcgc ctgcgacgcg accgaccggg ccttgcgcag gtgatccagg gacgacaggt
     3421 accatcgccc caggggagcg atgagcgtct cggcgccgtg gcgatggccg gcccgcagtt
     3481 cggccagcac cgaatccgcg gcgcggtcca tcgccgccgc gtcggccgcc acggtgtcca
     3541 cctgcgcgag caccc
//
LOCUS       DASUAP010000384         6427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_204108, whole genome shotgun sequence.
ACCESSION   DASUAP010000384 DASUAP010000000
VERSION     DASUAP010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204108"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFU45_10275"
     CDS             complement(<1..668)
                     /locus_tag="VFU45_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5359497.1"
                     /translation="MRTIQTLERDLARAAVLAALLLGAAAAPAAAQDPRPARSPSDTR
                     RTALVDAAARVSPSVVSISVQSHQRTTSQSPFDLFFIPQVTERAVTTYGTGFVIRSGG
                     YIVTNQHVVANAESITVTLPDGTDLPATLVGADPLSDIAVVKVDRRGLPVPPMGRTSD
                     LMIGEWVVALGNPFNFLLGNAEPTVTAGVVSATGRNILPQGDETGLYLDMIQTDAAIN
                     HGNSG"
     gene            complement(640..1425)
                     /gene="truA"
                     /locus_tag="VFU45_10280"
     CDS             complement(640..1425)
                     /gene="truA"
                     /locus_tag="VFU45_10280"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU5359498.1"
                     /translation="MTRTFLARLHYAGGGFLGWQRQAEGRTVQGEFERVLERLVGHRV
                     AAHAAGRTDAGVHAIGMGVSFTLPERWDSEAVARALNGLLPSDCWVESVERARSGFHA
                     RRSATGRRYRYDIGTDLAARSPFRRPYEWALCRPLDHAALDAAAAQVRGTHDFSAFAV
                     RGEPKPHYQCTLRQAEWERRPDGAGARFHVAADRFLHHMVRMLVGTMVDIALGRRPAA
                     DMGALLAAGDNRETSPPAPPQGLYFVAADYPAECFADDPDPRT"
     gene            1561..2601
                     /gene="trpD"
                     /locus_tag="VFU45_10285"
     CDS             1561..2601
                     /gene="trpD"
                     /locus_tag="VFU45_10285"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU5359499.1"
                     /translation="MNRSPDRSDPLSRAIVELAHGRHLSADAAGAAFDELMQGHATPA
                     QVAALLVGLRVLGETGDEVAGAAAALRRAMVPLAGIDRPAELVDTCGTGGGTVPTFNI
                     STAAALVAAGAGVRVAKHGNRSFTSKSGSADVFEALGVAIDVTPDVAARTLARIGIAF
                     LYAPTYHPALRHAGPVRRELGIPTVMNLVGPLGNPAGARRQVVGVADRARGPVLARAL
                     ARLGALHALVVHGEAGMDEVSPVGPTAIWEVRDGEVREWTLDLAEVGVAAVDPAALAG
                     GTPAENARRVEGLLRGGADGPARAAVLLNAAAAIYVSAGKRTFREALAEAGRALDDGR
                     AAERLDRLRAGA"
     gene            complement(2604..3212)
                     /gene="lexA"
                     /locus_tag="VFU45_10290"
     CDS             complement(2604..3212)
                     /gene="lexA"
                     /locus_tag="VFU45_10290"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU5359500.1"
                     /translation="MPLTKRQSEILEFVRGYIAEQGYAPSFEEIAGRFEFQSLATVHE
                     HLSNLERKGYIRRAHNESRAIEVLPPRGQAIATEIPLLGTVAAGLPIESLAHDETVAV
                     PDDLLPRRGPNYALRVRGQSMIDEHICDGDVVVVHGREAAENGEMVIALVNGAEATVK
                     KYYREPGGWVRLQPANATMSPMRFQERDVVIQGVVVGVLRKY"
     gene            3251..4075
                     /locus_tag="VFU45_10295"
     CDS             3251..4075
                     /locus_tag="VFU45_10295"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU5359501.1"
                     /translation="MNLPEHGPKGKSGDVLAGILAETRTRIPALRLRQAELERAAAAR
                     PDPPSFRAALRAGDGLALIAEVKRRSPSEGVISSRLDPVAHARSYAAAGARAISVLTE
                     PTRFGGSIDDLEAVVARVPTPVLRKDFIVDEIQLVEARAAGAAAVLLIVRALSARELP
                     ALLRAANGLGLGTLVEAHSALEVDAALEAGAMIIGVNSRDLADLRIDRDAAWALLARV
                     PADRVAVAESGIASAADAAQAAAAGADAVLVGSALSRSASPAPLVASIVGVPRRGR"
     gene            4065..4712
                     /locus_tag="VFU45_10300"
     CDS             4065..4712
                     /locus_tag="VFU45_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU5359502.1"
                     /translation="MAVEAKICGIRRAEDAAVAVAGGASYLGVILVPGSRRTVGVQEA
                     REIVVAAAGRPVFGVFATDAADAILNMRDATGIRGAQLHGSCSMHTAKRLRGEGMLVW
                     RVARVADRVDAAAVADGSAAVDAVLVEPRVEGALGGTGTALDRELGRLARSALHGRKM
                     VLAGGLRPGTVAAAVAAIGPDVVDVSSGVETVPGTKDPALIRSFLEALVGDHPSP"
     gene            4690..5892
                     /gene="trpB"
                     /locus_tag="VFU45_10305"
     CDS             4690..5892
                     /gene="trpB"
                     /locus_tag="VFU45_10305"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5359503.1"
                     /translation="MAITLPPERAVAGRFGPYGGRYVPETLMPALEQLAALYDSVRSD
                     PAFWAEYESILREFVGRPTPLTDAARFGAAAGCRVVLKREDLNHTGAHKINNTIGQAL
                     LARRMGKRRVIAETGAGQHGVATATVAARFGLECVVYMGAEDVARQALNVYRMRLLGA
                     TVVPVESGTRTLKDATNEALRDWVASVPTTHYIIGSVVGPDPYPRMVRDFQSVIGREA
                     RAQMLERHGKLPRTVVACVGGGSNAAGMFSGFLDDRSVELVGVEAAGDGVGTGRHSAT
                     LSAGRPGVLHGSLSYLLQDADGQVVPAHSVSAGLDYPGVGPEHSWHKDSGRVTYDAVT
                     DLEALAGFRRLAELEGIIPALETAHAVAWIERSRGRWAADDVVLLCLSGRGDKDVAHV
                     ASLAGVPA"
     gene            5895..>6427
                     /locus_tag="VFU45_10310"
     CDS             5895..>6427
                     /locus_tag="VFU45_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359504.1"
                     /translation="MTQGGDAPEVLLPPEQVAELLRTVTKALRATQIYLPNNPIYQRA
                     VEGVRAAFQPIWEVLDDLSFSITESDFIWDEQSVYNQASKSDSLAWTLFKDGLRLLTF
                     RPGVEADEIIRFLEVINKVRSLPSEAEDDLLTLLWEQDFQLIHYTFSEVLTDAAPPAV
                     SGPPVAGFVVGGTDAPVT"
BASE COUNT          838 a         2235 c         2439 g          915 t
ORIGIN      
        1 cccgaattgc cgtggttgat cgcggcgtcg gtctggatca tgtcgaggta gaggccggtc
       61 tcgtcgccct ggggcaggat gttccgcccc gtggcgctca ccacgccggc ggtgacggtc
      121 ggctccgcgt tcccgagcag gaagttgaac gggttgccaa gggccaccac ccactcgccg
      181 atcatgaggt cggacgtgcg gcccatcggc gggaccggga ggccgcggcg gtcgaccttg
      241 accacggcga tgtcggacag gggatcggcg ccgacgagcg tggccggcag gtcggtcccg
      301 tccgggaggg tgacggtgat cgactcggcg ttggccacca catgctggtt ggtcacgatg
      361 tacccgccgg accggatcac gaaccccgtg ccgtaggtgg tcaccgcccg ctcggtgacc
      421 tgcgggatga agaagaggtc gaacgggctc tgcgaggtgg tgcgctggtg ggactgcacg
      481 ctgatcgaga ccaccgacgg gctcacgcgg gccgcggcgt ccaccagcgc ggtgcggcgg
      541 gtgtcggagg gggaacgggc gggccgtgga tcctgcgccg ccgcaggagc ggccgccgct
      601 ccgagtagca gcgccgcgag cacggccgcg cgcgccaggt cacgttcgag ggtctggatc
      661 gtccgcaaag cactccgccg ggtagtccgc cgccacgaag tagagcccct gcgggggggc
      721 cgggggactc gtctcccggt tgtcccccgc cgccagcagg gcgcccatgt ctgccgccgg
      781 tcgccgtcca agggcgatgt ccaccatcgt ccccacgagc atccgcacca tatggtgcag
      841 gaaccggtcg gccgccacgt ggaaccgcgc cccggcaccg tccggccggc gctcccactc
      901 cgcctggcgc agcgtgcact ggtagtgcgg tttcggctca ccccgcaccg cgaatgcgga
      961 gaagtcgtgc gtcccgcgga cctgggccgc ggcggcgtcc agggcggcgt ggtcgagggg
     1021 gcggcagagc gcccactcat acggccgccg gaagggcgat cgcgccgcga ggtcggtgcc
     1081 gatgtcgtat cggtagcggc gacccgtcgc gctccggcgc gcgtggaagc ccgaccgcgc
     1141 ccgctcgacc gactcgaccc agcaatccga cggcagcagg ccgttgagcg cccgcgccac
     1201 cgcctcgctg tcccaccgct cggggagggt gaagctcacc cccatcccga tcgcgtgcac
     1261 tcccgcgtcg gtccgtcctg cggcatgcgc ggccacccgg tggccgacca gccgctcgag
     1321 gacccgttcg aactcaccct ggaccgtgcg cccctcggcc tgacgctgcc atccgaggaa
     1381 gccgcccccg gcgtagtgca gccgggctag aaaggtgcgg gtcatcgcgg cggaatctag
     1441 cccgcacggg ctcctcctac aagcgccgca actcgttgtc agcgttgaca aaagccatca
     1501 tcgaccggta cactccttga gcctcgtgct cccctcccgc cgccctctcc gcgccacctg
     1561 atgaatcgtt cacccgaccg atccgatccc ctgtcgcgcg ccatcgtcga gctggcccac
     1621 ggccgtcacc tctcggccga cgccgccggg gcggcgttcg acgaactgat gcaggggcac
     1681 gccacccccg cacaggtcgc ggcgctgctg gtggggctgc gcgtgctggg ggagaccggc
     1741 gacgaggtcg ccggcgccgc cgcggcgctt cgccgggcga tggtgccgct ggcagggatc
     1801 gaccgcccgg ctgaactggt cgacacctgc ggcaccggcg gcggcacggt gccgaccttc
     1861 aacatctcga cggcggcggc cctcgtcgcc gccggtgctg gggtccgggt ggccaagcac
     1921 gggaaccgga gcttcacgtc gaagagcggc tcggcggacg tcttcgaggc gctcggcgtg
     1981 gccatcgacg tcacgcccga cgtggcagcc cggacgctcg cgcgcatcgg catcgccttc
     2041 ctctacgctc ccacctacca cccggcactc cggcacgccg gcccggtgcg ccgggaactc
     2101 ggcatcccca cggtgatgaa tctcgtggga cccctcggca accccgcagg tgcccgccgg
     2161 caggtggtgg gcgtcgccga ccgcgcccgc ggcccggtgc tcgcgcgggc gctcgcccgc
     2221 ctcggcgcgc tccacgccct cgtggtccac ggcgaagcgg ggatggacga ggtaagtccg
     2281 gtgggtccca ccgccatctg ggaggtccgg gacggggagg tgcgggagtg gacgctcgac
     2341 ctggccgagg tgggcgtggc ggcggtcgac ccggccgcgc tggcgggtgg aactccggcg
     2401 gagaacgcac ggcgggtcga agggttgttg cggggtgggg cggacggtcc ggcgcgggcg
     2461 gcggtgctgc tcaatgctgc cgcggcgatc tacgtgtcag cggggaagcg cacgttccgc
     2521 gaggcactgg cggaagcggg ccgggccctc gacgacggcc gggccgccga gcggctcgac
     2581 cggctgcggg cgggcgcgtg aggtcagtac ttgcggagca cgccgaccac gacgccctgg
     2641 atcacgacat cgcgctcctg gaagcgcatc gggctcatcg tggcgttggc cggctgcagg
     2701 cggacccagc cacccggctc gcggtagtac ttcttgacgg tggcctcggc gccattgacc
     2761 agtgcgatga ccatttcgcc gttctcggcg gcctcgcggc cgtggaccac gacgacatcg
     2821 ccatcgcaga tgtgctcgtc gatcatcgac tggccccgca cgcgcagggc gtagttcggc
     2881 ccgcggcgcg gcaggaggtc gtccggcacg gcgacggtct cgtcgtgcgc cagcgactcg
     2941 atcggaaggc ccgccgcgac cgtgccgagg agcgggatct cggtcgcgat ggcctggccg
     3001 cggggcggaa gcacctcgat ggcccggctc tcgttgtggg cccggcggat gtagcccttc
     3061 cgctccaggt tgctgagatg ctcgtggacg gtggcgagcg actggaactc gaaccggccg
     3121 gcgatctcct cgaagctggg cgcatacccc tgctcggcga tgtagccgcg gacgaattcg
     3181 aggatctcgc tctggcgctt ggtgagcggc atcgctcccc cgggtcgatg ggtgaccgaa
     3241 cggactccga atgaacttac ccgaacatgg gccgaagggc aagagtggcg atgtcctggc
     3301 cgggatcctg gcggagaccc ggacgcggat cccggcgctc cggcttcggc aggccgagct
     3361 ggagcgcgcg gcggccgcgc gccccgatcc gccgtcattc cgcgccgcac tgcgggcggg
     3421 cgacgggctc gcgctgatcg cggaggtcaa gcggcgctct ccgtcggagg gggtcatctc
     3481 gtcgcggctc gacccggtgg cgcacgcccg atcctatgcc gcggccggag cgcgggccat
     3541 ttccgtgctc accgagccga cccggttcgg cggctcgatc gacgacctcg aggccgtcgt
     3601 cgcgcgggtc ccgacgccgg tgctccgcaa ggacttcatc gtcgatgaga tccagctggt
     3661 ggaagcgcgc gcggcggggg ccgcggcggt gctgctcatc gtgcgggcgc tctccgcccg
     3721 ggagctgccg gccctccttc gcgccgccaa cggcctgggg cttggcacgc tggtcgaggc
     3781 gcactccgcc ctcgaggtcg acgccgcgct cgaggccggc gcgatgatca tcggcgtcaa
     3841 cagccgggat cttgccgacc tccggatcga tcgggacgct gcgtgggcgt tgctggcgcg
     3901 ggtgccggcg gaccgggtgg cggtggcgga gagcgggatc gcatcggccg cggacgccgc
     3961 ccaggccgcc gccgcagggg cggacgccgt gctggtcggg agcgccctat cccgcagcgc
     4021 ctccccggcg ccactggtgg cttcgatcgt gggggtgccg cgccgtggcc gttgaggcga
     4081 agatctgcgg gatccgccgg gccgaggatg ccgcggtagc cgtggcgggc ggagcatcgt
     4141 accttggtgt tatcctggtg ccgggctccc ggcgcacagt gggcgtgcag gaggcgcgcg
     4201 agatcgtcgt cgccgcggcc ggtcgcccgg tcttcggggt cttcgcgacc gacgctgccg
     4261 acgctatcct caacatgcga gatgcgaccg ggatccgcgg agcccagctg cacgggtcgt
     4321 gctcgatgca taccgccaag cgcctgcgcg gggaggggat gctggtgtgg cgcgtggcgc
     4381 gggtggccga ccgggtcgac gcggcggcgg tggcggatgg atcggcggcg gtcgacgcgg
     4441 tgctggtgga gccgcgggtg gagggcgcgc tgggcgggac cggcacggcg ctcgaccgcg
     4501 agctcggccg cctggcccgg tcggcgcttc acggccggaa gatggtgctc gccggcgggc
     4561 tccggcccgg gaccgtggcc gcggcggtcg ccgcgatcgg gcccgatgtg gtggacgtca
     4621 gctccggggt cgagaccgtc cccggcacga aggatcctgc actgatcagg tcgttcctgg
     4681 aggcgctggt tggcgatcac ccttccccct gaacgggcgg tcgcaggccg gttcgggccg
     4741 tacggtgggc ggtacgtgcc tgagaccctc atgcctgcgc tcgaacagct cgccgcactg
     4801 tatgattccg tgcggagcga ccctgcgttc tgggcggagt atgagtcgat actacgcgag
     4861 ttcgtcgggc ggccgacgcc gctcaccgat gcggcgcgct tcggcgcggc ggccgggtgc
     4921 cgcgtcgtgc tcaagcgcga ggacttgaac cacaccggcg cccacaagat caacaacacc
     4981 atcggccagg cgctcctggc gcggcggatg gggaagcggc gcgtcatcgc cgagacgggc
     5041 gcgggtcagc acggtgtggc caccgccacc gtggccgccc gtttcgggct cgagtgcgtg
     5101 gtgtacatgg gcgcggagga cgtggcgcgc caggcgctca acgtgtaccg gatgcggttg
     5161 ctgggcgcca cggtggtgcc ggtcgagtcg ggcacccgga ccctcaagga cgccaccaac
     5221 gaggcgctgc gggactgggt cgcgagcgtc ccgaccaccc actacatcat cggttcggtg
     5281 gtggggccgg acccgtaccc gcggatggtg cgcgacttcc agtcggtcat cgggcgcgag
     5341 gcgcgcgccc agatgctcga gcgccacgga aagctcccgc ggaccgtcgt cgcatgcgtc
     5401 ggcggggggt cgaatgccgc cggcatgttc agcggttttc tcgacgaccg gtcggtcgag
     5461 ctggtggggg tcgaggccgc cggcgacggc gtgggcaccg ggcgccacag cgccacactt
     5521 tcggcggggc ggccgggggt gctgcacggg tcgctgagct acctcctcca ggacgccgat
     5581 ggccaggtgg tgccggcgca ctcggtgtcg gcggggctcg actatccggg ggtggggccg
     5641 gagcacagct ggcacaagga cagcgggcgg gtgacgtacg acgccgtcac cgacctcgag
     5701 gcgctcgccg gcttccgccg gctggcggaa ctcgagggga tcatcccggc gctcgagacc
     5761 gcgcacgcgg tggcgtggat cgagcgctcc cgtgggcggt gggcggccga cgacgtggtg
     5821 ctgctctgcc tgagcggccg cggcgacaag gacgtggccc acgtggcgtc gctcgccggg
     5881 gtgccggcgt gacgatgacg cagggcggcg acgcgcccga ggtcctgctg ccgccggagc
     5941 aggtggccga actgctgcgc acggtgacca aggcgctccg ggccacccag atctacctcc
     6001 ccaacaaccc gatctaccag cgggcggtcg aaggggtccg tgctgcgttc cagccgatct
     6061 gggaagtcct cgacgacctg tcgttctcca tcaccgagtc cgacttcatc tgggacgagc
     6121 agtcggtcta caaccaggcc tcgaagtcgg acagcctggc gtggacgctg ttcaaggacg
     6181 ggctccggct cctgaccttc cggcccggcg tcgaggccga cgagatcatc cggttcctgg
     6241 aggtcatcaa caaggtccgc tcgctgccgt cggaggcgga ggacgacctc ctcacgctgc
     6301 tctgggagca ggatttccag ctcatccact acacgttcag cgaggtgctg accgacgcgg
     6361 cgccgccggc ggtgagcgga ccgccggtgg cggggttcgt ggtgggcggg accgacgcgc
     6421 ccgtcac
//
LOCUS       DASUAP010000385         6745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_422359, whole genome shotgun sequence.
ACCESSION   DASUAP010000385 DASUAP010000000
VERSION     DASUAP010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422359"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..1545)
                     /locus_tag="VFU45_10315"
     CDS             complement(274..1545)
                     /locus_tag="VFU45_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5359505.1"
                     /translation="MADATALRDRLVQRSVGAPYSLFLGLGALLAVVGLGLFALSASG
                     PDADRAWHLFHVNWLFFTSLSAGGVVVSAVYKTAHAKWHGVIIRFAEACGAFLPVSFL
                     GFLVIFTAGYPHIFGHLQEQLPTLSAGKHLWLSRPWMFWRLFIALGALYAIGLRFVWH
                     NLLGDIHAAKGVVSGRRRAQYEKWTATYDAAKVEERLTRLAPLYVWVYAFAFTILAFD
                     TVMGLQPHWFSNLFGGFFFMGAFLGSQAALALLMMYGGRQLGISDLISDKQRHDLGKL
                     IFGFSVFWTYLMWSQFLVIWYGNMPEETGFVFARLWGHWEMVGRLVFLGLFLIPFIGL
                     LGVSPKKYPVTLGFFSAIIVLTLWLERYLLVLPSVTAQDGPVFGLPELGPTLAFLGLF
                     LLAYALFARTFPMVSPRLAMITLDKERGGHH"
     gene            complement(1545..2147)
                     /locus_tag="VFU45_10320"
     CDS             complement(1545..2147)
                     /locus_tag="VFU45_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5359506.1"
                     /translation="MPRKLLATLATGGLLLTGGCNWYYNTLPGPDDLMHAIPWFDHMI
                     VQASVHPYQFADVPRYTPAGTVPVTGGERDWGTGNPLMLQFAFDTLVADRLPNPSDKT
                     ATLARGDTVYHTFCAVCHGPAGDPAAATVGPRMGALSLLTPRARAYKDGYLYSMIRYG
                     RGVMPQYGDKIRPDDRWAVVNYVRTLQAAAADTGHAGGKN"
     gene            complement(2137..2646)
                     /locus_tag="VFU45_10325"
     CDS             complement(2137..2646)
                     /locus_tag="VFU45_10325"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEU5359507.1"
                     /translation="MRKVVPGVLGTFAHIDAAAEAIRTLRAQGHRDLTVYSACAQHEI
                     EAALEQKVSPVRVFTLVGGITGCAAGLAMTFWMSLDWPLLVGGKPIATVPPYVVIMFE
                     LTVLFGSLITVAAVAGLSLLRPTTGMAYDESFSDDKVGVFVACRPDQQPAVERILSGA
                     GSVEVRRAA"
     gene            complement(2646..3989)
                     /gene="nrfD"
                     /locus_tag="VFU45_10330"
     CDS             complement(2646..3989)
                     /gene="nrfD"
                     /locus_tag="VFU45_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5359508.1"
                     /translation="MATTAYEPPTSQTHAEVTRDVLATLQAPGRGYYYLLGGALFALA
                     IGLVTFVVLLRDGLGHAGYNPPVMWSVYITTFVFWVGIGHAGTLISAILFLFRSPWRT
                     AVYRSTEAMTVFAVMTAGLFPIIHIGRQWVFYWLIPYPNQRYLWPNFKSPLIWDVFAI
                     STYLTVSTTFLVFGLVPDVAAVRDKVRGWRKKVYGMMAFGWKGTDQQWRHYSRAYLYL
                     AAMATPLVLSVHSTVSWDFAMGLIPGWHGTIFAPYFVAGAIYSGVGMVFTLVIPLRKV
                     LGLEHMITEYHFDNLAKLTLLVGSILLYSYSMEYFISWYSGNTFEQTTFFRRAFGPQW
                     WSGWTMITCNALLSQLLWFRKIRTNLTALFVLSIFINIGMWFERWVIIASSLSNDFNP
                     WAWGILNPTWADWGILIGSFGWFSMYFLLFFKTFPIVAIQEIKETIPMPRRKGAH"
     gene            complement(3992..>6745)
                     /locus_tag="VFU45_10335"
     CDS             complement(3992..>6745)
                     /locus_tag="VFU45_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5359509.1"
                     /translation="NPAHPVNEGTICSRGQSALQGLYNPDRLAGPMQKSGSGWKSISW
                     DDAIALLATKLGAAGGKVAVLSGAGRGTFDDLLGDWTRALGGEVVHFQPFDHEPLRLA
                     NQQVFGLDQLPFHDFARAKHIVSFGADFLETWLAPTEQARGFAASHGFAGGTAARHVF
                     IGPRMSLTGMNADEWQAVAPGSEAVLALSMANVILSERSGAPADANQLRGMLAAYTPE
                     KAGQATGLQPAVVQRLAREFASAKPSLAVAGGVNGQHREALELCAAVNLLNYVAGNVG
                     ETVVFGADLQHGDGYGAVEALFARMDQGQVAVALVHEANPLYALPQAGGFAARFAKVP
                     FKVSTSLWLDETAAACDLILPNLHALERWDDLRPRAGVTGLLQPAMEPVFPGRHTGDV
                     LLQVARKLGGALARFSAPDFKSHLQDAWAQFARKRGQADAVGFWRDALQRGGLYDPRP
                     VTTVRLAAGARTVSAKGAALDGDGDFVLLPAAGSMLFDGRGANRPWLLEMSDPVTKIT
                     WNSWVEVHPDTAQALDVREGEILRLTSPHGTIDAAVYVYPGIIPGALSVPLGWGHTEY
                     GRYATGRGANALDLIDARDGNGYLPYVSTRVRARKTGEYRQVAKTEGSNRQLGRGIAD
                     AMPLAAARKGLTLKEAYAAAGAPEVEGNPENEVKAIAGFRESVLQKQQHGGYADPHPK
                     WGMVVDLDKCTGCSACVTACYAENNIPTVGEDDILRGREMTWMRIERYYEGGSDGERL
                     ETRFVPMLCQQCDNAPCEPVCPVYASYHTVDGLNGQVYNRCVGTRYCSNNCPFKVRYF
                     NWYAYAKRAFPEPLNLQLNPDVTVRARGVMEKCTFCVHRIRGAQHDARMQGRELKDGD
                     VVTACQQACPSGAIVFGNVKDPSSKVVQAAAASSRGYHIFDELNVRSSINYLAKVNRP
                     AEA"
BASE COUNT         1113 a         2231 c         2450 g          951 t
ORIGIN      
        1 agcctccgcc agggcgaccg catgctcttc atgaccgcgc gggtgtccca ggactccctg
       61 cgggcgatgg tcgggaagct gatgatgcga tagagcgctt agggcttagg gcttggggaa
      121 tagggcgtag ggctgagtcg aaggagcgga acgaggggcc cggcagcggg ccctttctgc
      181 gttcggggga acgactgagg ggcactccat tcggaatgcc cctcacgcta cccactaagc
      241 cctaagcgct aagccccaag cgttacgcac ccatcaatgg tgcccgcccc gctccttgtc
      301 cagcgtgatc atcgccagcc ggggcgagac catcgggaac gtgcgcgcga agagggcgta
      361 ggccagcagg aagaggccca ggaacgcaag cgtgggcccg agctccggga ggccgaacac
      421 cgggccgtcc tgtgcggtga ccgacggcag caccaggagg tagcgttcga gccagagcgt
      481 gagcacgatg atggcggaga agaacccgag ggtgaccgga tacttctttg gcgacacgcc
      541 caggagcccg atgaagggga tgaggaagag cccgaggaac acgagccggc ccaccatctc
      601 ccagtggccc cagaggcggg cgaagacgaa ccccgtctcc tccggcatgt tgccgtacca
      661 gatgacgagg aactgggacc acatcaggta ggtccagaac accgagaagc cgaagatgag
      721 cttcccgagg tcgtggcgct gcttgtcgct gatgaggtcg ctgatgccga gctgccggcc
      781 gccgtacatc atcaggagcg cgagcgcggc ctgcgatccc aggaaggcgc ccatgaagaa
      841 gaagccgccg aagaggttcg agaaccagtg cggctggagc cccatcaccg tgtcgaacgc
      901 gaggatggtg aacgcgaagg cgtacaccca cacgtagagc ggcgcgaggc gcgtcaggcg
      961 ctcctcgacc ttggccgcgt cgtacgtggc ggtccacttc tcgtactgtg cccggcgccg
     1021 gccgctcacg acgcccttcg cggcgtggat gtcgccgagg aggttgtgcc agacgaaccg
     1081 caggccgatg gcgtagagcg ctccgagggc gatgaacagc cgccagaaca tccacggccg
     1141 cgagagccac aggtgcttgc cggccgagag cgtcggcagc tgctcctgga ggtggccgaa
     1201 gatgtgcggg tagccggccg tgaagatgac gaggaacccg aggaacgaca ccgggaggaa
     1261 cgcgccgcac gcctcggcga accggatgat gaccccgtgc cacttggcgt gcgcggtctt
     1321 gtacacggcc gacacgacca cgccgccggc gctcaggctg gtgaagaaga gccagttgac
     1381 gtggaacagg tgccaggcgc ggtcggcgtc gggcccggag gcgctcaggg caaagagccc
     1441 gagaccgacg accgcaagca gggcgccgag gcccaggaag agcgagtacg gcgcgccgac
     1501 ggagcgctgg accaggcggt cacgcagggc ggtggcgtcg gccatcagtt cttccctccg
     1561 gcgtggccgg tgtcggcggc cgccgcctgg agcgtgcgga cgtagttgac gacggcccag
     1621 cggtcatccg ggcggatctt gtcgccgtac tgcggcatca cgccgcgacc gtagcggatc
     1681 atgctgtaca ggtagccgtc cttgtacgcc cgcgcccggg gcgtgaggag cgagagtgcg
     1741 cccatccgcg gcccgacggt ggcggcagcc gggtcgccgg ccgggccgtg gcagacggcg
     1801 cagaaggtgt gatagaccgt gtctccgcgg gcgagcgtcg ccgtcttgtc ggaggggttc
     1861 ggcagccggt cggccaccag ggtgtcgaag gcgaactgca gcatcagcgg gttccctgtg
     1921 ccccagtcgc gctcgccgcc ggtgaccggc accgtgccgg ccggggtgta ccgcggcacg
     1981 tcggcgaact ggtacgggtg cacgctcgcc tggacgatca tatggtcgaa ccacgggatc
     2041 gcgtgcatca ggtcgtcggg gccggggagg gtgttgtagt accagttgca cccgccggtg
     2101 aggagcaggc ccccggtcgc gagcgtggcc aggagcttac gcggcacggc gcacctccac
     2161 ggacccggcg cccgacagga tccgctcgac cgcgggctgc tggtcggggc ggcaggccac
     2221 gaacacgccc accttgtcgt cggagaacga ttcgtcgtag gccatgcctg tcgtgggtcg
     2281 caggagggag agccccgcca ccgcggccac ggtgatgagc gacccgaaga gcaccgtcaa
     2341 ctcgaacatg atgacgacgt acggcggcac ggtggcgatc ggcttgccgc ccaccaggag
     2401 cggccagtcg agcgacatcc agaacgtcat cgcgaggccc gccgcgcagc cggtgatgcc
     2461 gcccacgagc gtgaacacgc ggaccgggct caccttctgc tccagcgccg cctcgatctc
     2521 gtgctgcgcg caggccgagt acaccgtgag gtcccggtgc ccttgcgccc gcagggtgcg
     2581 gatcgcctcg gccgcggcgt cgatgtgggc aaaggtgccc agcacgccgg gaacgacctt
     2641 gcgcatcagt gggccccctt tcgccgcggc atcgggatgg tctccttgat ctcctggatc
     2701 gccacgatcg ggaaggtctt gaagaagagg aggaagtaca tgctgaacca gccgaaggag
     2761 ccgatcagga tgccccagtc ggcccaggtc gggttcagga tgccccacgc ccacgggttg
     2821 aagtcgttcg agagcgacga ggcgatgatg acccagcgct cgaaccacat cccgatattg
     2881 atgaagatcg agagcacgaa gagcgccgtc aggttggtgc ggatcttccg gaaccagagc
     2941 agctgcgaca ggagcgcgtt gcaggtgatc atcgtccacc cgctccacca ctgcggcccg
     3001 aacgcccggc ggaagaacgt cgtctgctcg aacgtgttcc cgctgtacca ggagatgaaa
     3061 tactccatgg aataggagta gagcaggatc gacccgacca gcagcgtgag cttggccagg
     3121 ttgtcgaagt gatactcggt gatcatgtgc tcgagcccga gcaccttgcg cagcgggatc
     3181 accagcgtga acaccatccc gacgccggaa tagatggcgc cggccacgaa gtagggggcg
     3241 aagatggtcc cgtgccagcc ggggatgagc cccatggcga agtcccacga cacggtggag
     3301 tgcaccgaga gcaccagcgg ggtggccatc gcggccaggt agaggtaggc ccgcgagtag
     3361 tggcgccact gctggtcggt gcccttccag ccgaaggcca tcatcccgta caccttcttc
     3421 cgccagcccc gcaccttgtc gcgcacggcg gccacgtccg gcaccagccc gaagacgagg
     3481 aacgtggtcg acaccgtgag gtaggtcgag atggcgaaga cgtcccagat cagcggcgac
     3541 ttgaagttgg gccagaggta gcgctggttc gggtacggga tgagccagta gaacacccac
     3601 tggcgcccga tgtggatgat cgggaagagc ccggcggtca tcaccgcgaa gacggtcatc
     3661 gcctcggtgg accggtagac cgccgtccgc cagggcgagc ggaacaggaa caggatggcc
     3721 gagatgagcg tgccggcgtg gccgatgccg acccagaaga cgaacgtggt gatgtacacc
     3781 gaccacatca ccggcgggtt gtaccccgcg tgccccaggc cgtcgcggag caggaccacg
     3841 aaggtcacca gcccgatggc cagggcgaac agcgccccgc ccagcaggta gtagtagccg
     3901 cgccccggcg cctgcagcgt ggccaggacg tcccgggtga cctcggcgtg ggtctgggag
     3961 gtcggcggct cgtacgcggt ggtcgccatg ctcaggcctc cgccggccgg ttgaccttgg
     4021 ccaggtagtt gatcgacgat cgcacgttga gctcatcgaa gatgtggtag ccgcgcgagc
     4081 tcgccgcggc ggcctgcacc accttgctcg acgggtcctt gacgttgccg aacacgatgg
     4141 cgccggacgg gcaggcctgc tggcacgccg tcacgacgtc gccgtccttg agttcgcggc
     4201 cctgcatccg cgcatcgtgc tgggccccgc ggatccggtg cacgcagaag gtgcacttct
     4261 ccatgacgcc acgggcccgc accgtcacgt cggggttgag ctgcaggttg agcggctcgg
     4321 ggaacgcccg cttggcgtag gcgtaccagt tgaagtaccg gaccttgaag gggcagttgt
     4381 tggagcagta ccgcgtcccg acgcagcggt tgtacacctg gccgttgagc ccgtccaccg
     4441 tgtggtagga cgcgtagacc gggcagaccg gctcgcaggg cgcgttgtcg cactgctggc
     4501 agagcatggg cacgaaccgg gtctccagcc gctcgccgtc gctgccgccc tcgtagtagc
     4561 gctcgatccg catccacgtc atctcccggc cgcggaggat gtcatcctcg cccacggtgg
     4621 ggatgttgtt ctcggcgtag caggccgtga cgcaggcgga acagccggtg cacttgtcga
     4681 ggtcgaccac catcccccac ttgggatggg ggtcggcgta gccgccgtgc tgctgcttct
     4741 ggagcacgct ctcgcggaag ccggcgatgg ccttgacctc gttctccggg ttcccctcga
     4801 cctcgggggc gccggccgcg gcgtacgcct ccttgagcgt cagccccttc cgcgccgccg
     4861 cgagcggcat cgcgtcggcg atcccgcggc cgagctggcg gttggagccc tccgtcttgg
     4921 ccacctggcg gtactcgccc gtcttccgcg cgcgcacgcg ggtcgagacg tacgggaggt
     4981 agccgttgcc atcccgggcg tcgataaggt cgagcgcgtt ggccccgcga ccggtggcgt
     5041 accggccgta ctcggtgtgg ccccacccca gcggcaccga gagcgcgccg gggatgatgc
     5101 cgggatagac gtagaccgcc gcgtcgatgg tgccgtgggg ggacgtgagc cggaggatct
     5161 ccccttcgcg cacgtcgagc gcctgggcgg tgtcggggtg gacctcgacc cagctgttcc
     5221 acgtgatctt ggtgaccgga tcgctcatct cgaggagcca gggccggttg gccccgcgtc
     5281 cgtcgaagag cattgagccg gcggcgggca gcagcacgaa gtcgccgtcg ccgtcgaggg
     5341 ccgcaccctt ggcgctcacc gtgcgggcgc cggccgccag gcgcaccgtc gtgaccggcc
     5401 gggggtcgta gaggccgccc cgctgcagcg catcgcgcca gaacccgacg gcgtcggcct
     5461 ggccgcgctt ccgggcgaac tgggcccagg cgtcctgcag gtgggacttg aaatcggggg
     5521 cgctgaaccg cgcgagcgcg ccgcccagct tccgggccac ctggaggagc acgtcgccgg
     5581 tgtggcggcc ggggaagacc ggctccatcg ccggctggag gagaccggtg acgccggcgc
     5641 ggggccggag gtcgtcccag cgctcgagcg cgtgcaggtt gggaaggatc aggtcgcagg
     5701 cggcagcggt ctcgtcgagc cagagcgacg tcgacacctt gaacgggacc ttggcgaagc
     5761 gcgccgcgaa gccgcccgcc tggggcaggg cgtagagcgg gttcgcctcg tgcaccagcg
     5821 ccacggccac ctggccctgg tccatccggg cgaagagcgc ctcgacagcg ccgtagccgt
     5881 cgccgtgctg gaggtcggcg ccgaagacca ccgtctcccc cacgttgccg gcgacgtaat
     5941 tgaggaggtt gacggcggcg cagagctcga gtgcctcgcg gtgctggccg ttgacgccgc
     6001 ccgccaccgc gagcgacggc ttggccgagg cgaactcgcg cgccaggcgc tggaccaccg
     6061 cgggctgcag gccggtcgcc tggcccgcct tctcgggcgt gtaggcggcg agcatgccgc
     6121 ggagctggtt ggcatcggcc ggggcgccgc tgcgctccga gaggatcacg ttggccatgc
     6181 tgagcgccag caccgcctcg gagcccgggg ccaccgcctg ccactcgtcg gcgttcatcc
     6241 ccgtgagcga catccgcgga ccgatgaaca cgtggcgcgc ggcggtgccg ccggcgaagc
     6301 cgtgcgaggc cgcgaagccg cgcgcctgct cggtgggggc cagccaggtc tcgaggaagt
     6361 cagcgccgaa ggacacgatg tgcttcgccc tggcgaagtc gtggaacggc agctggtcga
     6421 ggccgaagac ctgctggttg gccagccgga gcggctcgtg gtcgaagggc tggaagtgca
     6481 ccacctcgcc gcccagggcc cgggtccagt cgcccagcag gtcgtcgaag gtgccgcgtc
     6541 ccgcgccgga gagcaccgcc accttgccgc ccgccgcccc cagcttcgtg gcgaggagcg
     6601 caatggcgtc gtcccaggag atgctcttcc acccgctgcc gctcttctgc atcggcccgg
     6661 ccaggcggtc cgggttatag agcccctgca gcgccgactg gccgcgggag cagatcgtcc
     6721 cctcgttgac cgggtgggcc gggtt
//
LOCUS       DASUAP010000386         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_422394, whole genome shotgun sequence.
ACCESSION   DASUAP010000386 DASUAP010000000
VERSION     DASUAP010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422394"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..639
                     /locus_tag="VFU45_10340"
     CDS             67..639
                     /locus_tag="VFU45_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5359510.1"
                     /translation="MTARHFVEAFRRRARVNELTAEDGERLDEIADRFVATLRGGGTL
                     YFAGNGGSAADAQHVATEYVVRYKADRRPLRAIALTTDTSLLTAAGNDLGFDHAFARQ
                     VEALCRPGDLLVLHSTSGNSPNLLLAAEAAKRIGVATVAFLGRDGGKLRHAVDDAYVV
                     RSDVTSEIQETHLAIEHLIVEVVERELIGT"
     gene            1065..1817
                     /locus_tag="VFU45_10345"
     CDS             1065..1817
                     /locus_tag="VFU45_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5359511.1"
                     /translation="MISLTGKQVLITGGSRGIGAAAAELFARAGADVAITFRSRRAEA
                     AKVTLAVEALGRKAWAFAGDHGDPAAVRAIAADLAKVMPKLDVAVINHGIWPAEEVAV
                     AKMDDARWRDTMRADLDGLFYFTRAALGMMRPGGTVILVSSTAGQRGEAFHADYAATK
                     GAVIALTKSLAVECAPDITVNCVAPGWVDTDMSAAALRGPERARIDATIPLRRPASAM
                     DVAGPILFLASPLARHITGEVLNVNGGSVLCG"
     gene            1977..>3075
                     /locus_tag="VFU45_10350"
     CDS             1977..>3075
                     /locus_tag="VFU45_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU5359512.1"
                     /translation="MPHPPLPIPDTVLDIARRLEGAGYETWCVGGAVRDHLLGDANAD
                     FDLATRATPDEVRKLFRRTVDVGIEFGTVGVLDAERKLHEVTTFRRDVSTDGRRAVVA
                     FGATLEEDLARRDFTINAIAYHPLHHEWRDPFDGFGDLDRKLVRAVGRAEERFREDYL
                     RILRGVRFAARFGFQVEPATWAAALAAAPGLDGLSAERVRDEWFKSLRTTRSLPRLVE
                     LWSAVMSPSRWMPELLPAAPRDPGADFPRDPVLLTVFLADRPAQLLERLKASRGEIDR
                     AAAISAGPAAPAGLDDVAIRRWLSGVTPPVAADLAALHALRTGHAPAWEPAMQRILDR
                     GDPIDRSALAVTGADLLDAGIPKGPALGRVLK"
BASE COUNT          445 a         1090 c         1105 g          435 t
ORIGIN      
        1 ccgggcggag cacgagccgg agacgctcgc gatcttcgcc gagcgcctgg cgttcgaggg
       61 ggcgcaatga ccgcgcggca cttcgtggag gcgttccggc gccgggcgcg ggtcaacgag
      121 ctcacggccg aggacggcga gcggctggac gagatcgccg accggttcgt tgcgaccctc
      181 cggggcgggg ggacgctcta ctttgccggc aacggcggga gcgcggcgga cgcccagcac
      241 gtggccacgg agtacgtggt gcgctacaag gccgaccggc ggcccctccg cgcgatcgcg
      301 ctcaccaccg acacgtcgct cctcaccgcg gcggggaacg acctgggatt cgaccatgcg
      361 ttcgcgcgcc aggtcgaggc gctctgccgc cccggcgacc tgctggtgct ccactcgacc
      421 agcggcaaca gcccgaacct cctcctcgcg gccgaagcgg ccaaacggat cggcgtggcg
      481 accgtggcgt tcctcgggcg ggacggcggg aagctccgcc acgcggtgga cgacgcctat
      541 gtggtgcggt cggacgtgac cagcgagatc caggagaccc acctcgccat cgagcacctg
      601 atcgtggaag tcgtggagcg ggagctgatc gggacgtgag agggaaaagt gaaaagggaa
      661 aagggaaaag ggaaaaggga aaatgggaaa agtgaaaagg gggctatgat gtggtgcaag
      721 cccccttgcg cgtcggtgag ggtcgcccgc ggaaaatgac ggccgtgggg gtcgcctgcg
      781 gcgacccgaa acgtcgccat cgttgcgtca tcccgagcgg agcgagggat ctcaaaccga
      841 tcgtcgctct tgtccgccga tgtcgccccg ctcccggcac cgccagcatc ggttcgagat
      901 ccttcggtcg cttcgctccc tcaggatgac tccggtgagc cggccactgg cggggatcgt
      961 tcgccgccca gacagcgccc tcttcccctt ctccttctcc ttctccttct cccttctcct
     1021 tttccctttt cccttttccc tcttcccttt cccctcccca caccatgatc tccctcaccg
     1081 gcaagcaggt cctcatcacc ggcggctccc gcggcatcgg cgcggcggcg gcggagctct
     1141 ttgcgcgcgc cggggccgac gtggcgatca ccttccgttc ccggcgggcc gaggcggcca
     1201 aggtgacgct cgcggtcgag gcgctgggcc ggaaggcgtg ggcctttgcc ggcgaccacg
     1261 gcgacccggc cgcggtgcgc gcgatcgcgg ccgacctggc caaggtgatg ccgaaactcg
     1321 acgtggcggt gatcaaccac gggatctggc cggcggaaga agtggcagtc gccaagatgg
     1381 atgacgcccg ctggcgcgac acgatgcgcg ccgacctcga cggccttttc tactttaccc
     1441 gtgcggcgct cggcatgatg cgcccgggcg ggaccgtcat cctagtctcg agcaccgcgg
     1501 ggcagcgagg cgaggcgttc cacgccgatt acgccgccac caagggggcg gtcatcgcgc
     1561 tcaccaagtc gctggcggtg gagtgcgcgc cggacatcac ggtcaattgc gtggcgccgg
     1621 gatgggtgga caccgacatg agcgccgccg cgctccgcgg ccccgagcgc gcgcggatcg
     1681 acgcgacgat cccgctccgc cgcccggcga gcgcgatgga cgtggcgggg ccgatcctgt
     1741 tcctcgcgag cccgctggcg cggcacatca ccggcgaagt gctcaacgtg aatggcggga
     1801 gcgtgctctg cgggtaggcg cgggaacggc cgggtgaaca gccgcgtgga ctgccgggcg
     1861 ggtgcatagg ccagcattca gcaggggcgg cgcaagcgcc gcccgtcagg atggagaggg
     1921 ggtagggggt gaggtgtcgg ggtggcagca ggccaccccc tactattatt cggccgatgc
     1981 cgcacccgcc cctccccatc cccgataccg tcctcgacat cgcccggcgc ctcgaaggcg
     2041 cggggtacga gacctggtgc gtcggcgggg cggtgcggga ccacctcctg ggcgacgcca
     2101 acgccgactt cgacctggcc acgcgcgcca ctcccgatga ggtgcggaag ctcttccggc
     2161 gcacggtgga cgtgggcatc gagttcggca cggtgggggt cctcgacgcg gagcggaagc
     2221 tccacgaagt gacgaccttc cggcgcgacg tgtcgaccga cgggcggcgg gcggtggtgg
     2281 ctttcggcgc cacgctcgag gaggacctgg cccggcgcga cttcacgatc aacgccatcg
     2341 cgtaccaccc gctccaccac gaatggcgcg acccattcga cggcttcggc gacctcgacc
     2401 ggaagctcgt gcgcgcggtg ggaagggcgg aggagcgctt ccgcgaggac tacctccgga
     2461 tcctccgcgg ggtgcgcttc gcggcgcgct tcggcttcca ggtggagccc gccacctggg
     2521 ccgcggcgct ggcggcggcc cccggactcg acggcctctc cgcggagcgc gtgcgggacg
     2581 aatggttcaa gagcctccgc accacccgct ccctgccgcg gctggtggag ttgtggagcg
     2641 cggtgatgtc cccctcacgc tggatgccgg aactgctccc cgcggcaccc cgcgaccccg
     2701 gcgccgactt tccccgcgat ccggtcctcc tcaccgtatt cctggccgat cggccggcac
     2761 agcttctgga gcgcctcaag gcctcgcgcg gcgagatcga ccgcgccgcg gcgatcagcg
     2821 cgggtccggc ggccccggcg ggcttggacg acgtggccat ccgccgctgg ctctcaggcg
     2881 tcacgccacc ggtagcagcg gacctggcgg cgctccacgc gctccggacc ggccacgcac
     2941 cggcgtggga gccggcgatg cagcggatcc tcgaccgggg cgacccgatc gaccgcagcg
     3001 ccctcgccgt gaccggcgcg gacctcctcg acgccgggat cccgaagggg ccggcgctcg
     3061 gccgggtgct caagg
//
LOCUS       DASUAP010000387         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_313365, whole genome shotgun sequence.
ACCESSION   DASUAP010000387 DASUAP010000000
VERSION     DASUAP010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313365"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2383)
                     /gene="dnaE"
                     /locus_tag="VFU45_10355"
     CDS             complement(<1..>2383)
                     /gene="dnaE"
                     /locus_tag="VFU45_10355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5359513.1"
                     /translation="YSLLDGANRIGDLVAHTQRLGMDSLAVTDHGNLHAAWTFYETAR
                     AARLRPILGFEAYLAFGPRQAREKPAGAPAAYSHLVLLARNREGYRNLVRLSSIGFTE
                     GFYRRPRIDREVLERYRDGLVCLAACLSGEVALHLRQQKYDEARATAQWLAGVFGPEN
                     FWLEIQKHGLPEEDLVTQGMLSLGRELGLGVVATNDAHYLRREDAEAHDVLLAIGTGA
                     DLDDPKRFRFTGTESYVKSEAEMRALFPQHPETIANTQLVADRCEFDFEKRYYVPGFP
                     RPPEFDSDEALLRHLTEEGAGRRYGAPLPDAVRARMDYELGVIETAGYASYFLIVQDF
                     IHAARERGIPVGPGRGSAAGSLVAYALGITNVCPLQFDLLFERFLNPERVSMPDIDVD
                     FCFERRGEVIEYVRQRYGRDSVGQIVTFGTMKARAAVKDVARVLRIPPGEADRLTKLI
                     PSGPAYSLSIPEAVQKVDELRDLVKANPAYTRLMDLSARIEGVSRHLSVHAAGVVIAP
                     GPLSEYVPVCTAPTRGAGAAADGEDAVITQFEMGPLEKVGMLKMDILGLKTLTVIHDA
                     VEMIRERRGVAVDIDAVPFDDPEVYRLLRAGRTAGVFQFESPLATETLRAMKCDRFDD
                     LVASNALLRPGPLDAGMHTVFIRRKLGQEKVTYPHPLLQEILEPTYGVITYQEQVMRI
                     ANVLAGFSLAEADVLRKAVGKKDAELIRKEIGRFVERAVAQGHPRKLAEELGAQIETF
                     GRYGFNKSHSVAYSVLSYQTAWLKAHYPAEFMAALLSSEIGNTDKVVQYINEAR"
BASE COUNT          370 a          815 c          818 g          380 t
ORIGIN      
        1 cgcgcctcgt tgatgtactg caccaccttg tcggtgttcc cgatttccga ggagagcagc
       61 gcggccatga actcggccgg gtagtgggcc ttgagccacg ccgtctggta gctcagcacc
      121 gaataggcca ccgagtgcga cttgttgaac ccgtaacggc cgaaggtctc gatctgcgcg
      181 cccagttcct cggccagctt ccgcggatgg ccctgggcca ccgcccgctc cacgaagcgg
      241 ccgatctcct tccggatgag ttccgcgtcc ttcttgccca ccgccttccg cagcacgtcg
      301 gcttccgcca gcgagaagcc ggccagcaca ttggcgatgc gcatcacctg ttcctggtag
      361 gtgatgacgc cgtaggtcgg ctcgaggatc tcctgcagca gcgggtgcgg gtaggtgacc
      421 ttctcctgtc ccagcttccg ccggatgaac acggtgtgca tgccggcgtc gagcggtccg
      481 gggcggagga gcgcgttgga ggcgaccagg tcgtcgaacc ggtcgcactt catggcgcgg
      541 agcgtctcgg tggcgagcgg cgactcgaac tggaagacgc cggcggtgcg gccggcacgg
      601 agcaggcgat agacctcggg atcgtcaaac ggcaccgcat cgatgtccac cgcgacgcca
      661 cgccgctccc ggatcatttc caccgcatcg tgaatcacgg tgagcgtctt gaggccgagg
      721 atgtccatct tgagcatccc gaccttctcc agcggcccca tctcgaactg cgtgatgacg
      781 gcgtcctcgc catccgccgc ggcaccggcg ccacgggtgg gcgcggtgca gaccggcaca
      841 tactccgaca gcgggccggg ggcgatcacc acgcccgccg catggaccga caggtgccgg
      901 ctcacgccct cgatccgggc actcaggtcc atcagccggg tgtaggccgg attggccttc
      961 accaggtcgc gcagttcgtc caccttctgc accgcttcgg gaatggacag gctgtaggcg
     1021 ggtcccgacg gaatgagctt ggtgaggcgg tccgcctccc ccggcggaat ccggaggacg
     1081 cgtgccacgt ccttgacggc ggcgcgcgcc ttcatggtcc cgaaggtcac gatctggccg
     1141 acgctgtccc gcccgtaccg ctggcgcacg tactcgatga cctcgccccg gcgctcgaag
     1201 cagaaatcca cgtcgatgtc cggcatcgag acgcgctcgg ggttgaggaa ccgctcgaac
     1261 agcaggtcga actgcagcgg gcagacgttg gtgatgccga gggcgtaggc gaccagcgac
     1321 ccggcggcgg agccgcggcc gggcccgacc ggaatccccc gctcccgcgc cgcgtggatg
     1381 aagtcctgca cgatgaggaa gtagctggca tagccggcgg tctcgatcac gccgagttcg
     1441 taatccatgc gggcgcggac cgcatcgggc aggggcgcgc cataccgtcg cccggctcct
     1501 tcctccgtca ggtggcggag cagggcctcg tcgctgtcga actcgggagg ccgcggaaag
     1561 ccggggacgt agtaccgctt ctcgaagtcg aactcgcacc ggtccgccac cagctgcgtg
     1621 ttggcgatcg tctccggatg ctgggggaac agcgcccgca tctccgcttc gctcttgacg
     1681 tacgactcgg tcccggtgaa gcggaaccgc ttcgggtcgt cgaggtccgc accggttccg
     1741 atggcgagca gcacgtcatg agcctcggcg tcctcgcgcc gcaggtagtg cgcgtcgttg
     1801 gtggccacca caccgaggcc gagctcccgg cccagggaca gcatgccctg ggtgacgaga
     1861 tcctcctccg gcaggccgtg cttctggatc tcgagccaga agttctccgg gccgaacaca
     1921 ccggcgagcc actgcgcggt agcgcgggct tcgtcgtact tctgctggcg gagatgcaat
     1981 gccacctcgc ccgagaggca cgcggcgagg cagaccagcc cgtcgcggta ccgctccagc
     2041 acctcccggt caatgcgggg ccgccggtag aacccttcgg tgaacccgat cgaggagagg
     2101 cggaccaggt tgcgatatcc ctcccggttt cgcgccagca gcacgaggtg gctgtaggcc
     2161 gccggtgcgc cggccggctt ctcgcgggcc tgccgcgggc cgaaggccag ataggcctcg
     2221 aagccgagga tcgggcgcag gcgagcggcc cgggcggttt cgtagaaagt ccacgccgcg
     2281 tgcaggttgc cgtggtcggt gaccgcgagc gaatccatgc ccaggcgctg ggtgtgcgcg
     2341 accaggtcgc cgatgcggtt ggcgccgtcc agcagcgagt act
//
LOCUS       DASUAP010000388        10187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2498
            k141_1253413, whole genome shotgun sequence.
ACCESSION   DASUAP010000388 DASUAP010000000
VERSION     DASUAP010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960955
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:43:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,081
            CDSs (total)                      :: 2,059
            Genes (coding)                    :: 2,055
            CDSs (with protein)               :: 2,055
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10187
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253413"
                     /isolate="SMAG_U2498"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFU45_10360"
     CDS             <1..667
                     /locus_tag="VFU45_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU5359514.1"
                     /translation="TGLRGPAAGSERIFADRVEAGVALAGALARYADPRMLVLGLVRG
                     GAPVAAEVARRLGAEFDVIVVRKLGAPGAPELAIGAITADGATVFNDDVIGELALDRA
                     QVAAVSATRRREAEELERAFHRRRPGAAVEGRTVILVDDGLATGATMRAAVASVRRRR
                     PGRVVVAVPVGSVEACAALRAEADEVVCLQEPVHFQAVGAWYGDFRQVTDGEVAALLP
                     EEG"
     gene            complement(671..2290)
                     /gene="groL"
                     /locus_tag="VFU45_10365"
     CDS             complement(671..2290)
                     /gene="groL"
                     /locus_tag="VFU45_10365"
                     /inference="COORDINATES: protein motif:HMM:NF009487.0"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5359515.1"
                     /translation="MTHKRLLFRSAAREKVLAGAAALADAVRVTLGPRSKCVLIERKW
                     GQPLVCDDGVTIAKEVDLEDPDETLGAQVLREAAARTGEAVGDGTTTSTLLAHAIFAE
                     GLRNVAAGASALDLRRGLERGSRAAVAALREISRPVTTRLEKAQVAALSAHNDAITGD
                     LVADAVERVGPEGVISVEEAKGTETAVEVVEGMQFDRGYLSPYFVTEAAKMEAVLQEP
                     FILLHDRRISNMQDLLPLLEQVAKMNRSLLVVAEEVEGEALATLVVNRIRGLLACVAV
                     KAPGFGDRRRAMLDDIGILTGGKVIAEETGAKLEHVTVADLGGAKRVVVDRDSTTIIG
                     GAGAATAIAARCEELRGQIRDTTSDYDREKLEERLAKLSGGVAVIRVGAPSETELKSR
                     REAFEDAIAATKAAVAEGIVPGGGLALLRCIPAVVRESEAVDGDERTGVLILRHALEA
                     PARQIAENSGIDGGVAVERMRTGDGTIGLDAARGTYVDLVAAGIVDPTKVVRVALENA
                     VSVAGTLLRTEATLTEMPEPAPELAHAGAPE"
     gene            complement(2287..2883)
                     /locus_tag="VFU45_10370"
     CDS             complement(2287..2883)
                     /locus_tag="VFU45_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEU5359516.1"
                     /translation="MATDLPGARAVPVRLAVRHAGLSPAAEAGIRRRAAHLARYFDRI
                     LACHVTVDVPQRRRRSDTLHRRVRLEVRVPGHALVVDRQPAAELATALDAAFHAMRRQ
                     LQDYVRRMREVGPPQAASPTGTVVEIYPLAGYGFLQAEDGEPVYFDRASVLHSAFERL
                     AVGSEVHYAEELGERGRQATTVALGSSRRQTGAGVAAE"
     gene            complement(2873..3082)
                     /locus_tag="VFU45_10375"
     CDS             complement(2873..3082)
                     /locus_tag="VFU45_10375"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEU5359517.1"
                     /translation="MSSIVKVVEVIGQSDKSFADAAQNAVKEVGKTVTDMKSVWIDNF
                     SGEIEGDRIKFFRVNAKVSFLVHGH"
     gene            complement(3234..4178)
                     /locus_tag="VFU45_10380"
     CDS             complement(3234..4178)
                     /locus_tag="VFU45_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU5359518.1"
                     /translation="MTLPGPPRRILLADHFRDALAEELRAMRPDLELRIRDRREVTRD
                     DLRWADCYLGFRPPTEVELEHVTWVHGTGAGLDAFLFRRHFPADVLLTRTSESFGAKM
                     GEYCLARMLAETQDLFAFEADQRARRWNTRDLRALTGTRALIVGTGDVGGGIASAIAA
                     LGVTVDGVSRSGALRAPFARTHRMEELPALAGGADWLVLAAPLTEATWHIAGAALFAA
                     CRGAYLINVGRGGLVDEPALLAAVEAGALRGAALDVFEAEPLPASSPLWAHPRIVISP
                     HVSGLSTVAHAARGFLDALTALERGERPAMAVDVSRGY"
     gene            complement(4175..5413)
                     /locus_tag="VFU45_10385"
     CDS             complement(4175..5413)
                     /locus_tag="VFU45_10385"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEU5359519.1"
                     /translation="MTSSWRKVVWLAAVAVSGIAPRAVAAQDTVSVPAGTHIPIRFLG
                     GVGPKHDRPGTPVTVQTMAAILHDACVVVRPFALVRGAVVPSGHRGRLALRFDSIAVT
                     RGRWVPLDAGLDSLEYAPQPIDSGAVTAGGKRGGKVGRALASIGIGAIDDELLFPAAI
                     LGGYALIRRRHPARIVAGELGGLRLRRPFAIEATADCVAPEAFPHLLTPLQLPTFVPR
                     TTDRRGRVTGDPINLVVLGPALLVDSAFRAAGWTEASTGNLLRISGEVAAMIADRRGS
                     EGAPVSAHYFSGRRQDTAYELEGSNARQRHHLRLWLVDTAQQAWVAAASRDAGLFVRP
                     LHGTATHRLDPDIDAERDLVVRELIAGGCAGLSGYTRLPGAVTSGRNAEGQRFLTDGR
                     TAVVHVHACRPPTPLPDDAL"
     gene            complement(5558..6496)
                     /locus_tag="VFU45_10390"
     CDS             complement(5558..6496)
                     /locus_tag="VFU45_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEU5359520.1"
                     /translation="MTATFIRPEISNAATAPGDPRIGHLLGGHVNGAAKPRVVIIGFP
                     SDEGVHRNGGRVGAAGAPMEIRRWLYRLTPDAQNHESYCELVERTLDLGDLKCSDDVA
                     ADQLALAEVLAPYLKDGVFPIVLGGGHETSFGHFLGYAGVGSKVEVINWDAHPDVRKL
                     LDEKGHSGSPFREALLHPSGACTRYTVAGLQPQSVAASHLNFLDDNRGRWIWNAELNA
                     ASVDALYAEADAPVMASFDIDAVDGSAAPGVSAPSVGGMTVDLWLHAAYQAGRSPKVT
                     SVDLVECNPVYDVDGRTARLAAVTIWQVLRGLAERE"
     gene            complement(6493..7740)
                     /gene="hutI"
                     /locus_tag="VFU45_10395"
     CDS             complement(6493..7740)
                     /gene="hutI"
                     /locus_tag="VFU45_10395"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEU5359521.1"
                     /translation="MPVLTNISTLYTCAGARQGDVGPIADGALAWDGDTIRWAGAARD
                     LPAEFRRWPREDAGGGMVIPGLIDCHTHLAFGGWRADDFERRIRGESYLQIAEAGGGI
                     ASTMRRTRALPEDGLVERAAGFLAGMVALGVTTVEAKSGYGLDLETELRILRAYRRLA
                     ERGPCRIVATFLGAHTVPPEYADRRAAYVSLVIDEMIPRVAGERLAEFCDVFVERSAF
                     SVDEARAVFRAATAAGLRPKLHADQLTSCGGAELAAEVGAASADHLEQVSDPGIAAMA
                     RSGVVAVSLPLATLYLGQAPQPSRKLIDAGVPVAVATDFNPGSAPSNHLPLALLLACT
                     LQRMAPAEALKGATLFAARAIGRDASVGSLVPGKRADFAVIDAPDVVQWLYHFRPNAC
                     RRTVIGGVTRWDRTSIAPRSTAP"
     gene            complement(7840..9513)
                     /gene="hutU"
                     /locus_tag="VFU45_10400"
     CDS             complement(7840..9513)
                     /gene="hutU"
                     /locus_tag="VFU45_10400"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU5359522.1"
                     /translation="MTATAAAPEIVRAPTGTTLSCKGWVQEAALRMLMNNLDPAVAEH
                     PDELIVYGGGGKAARNWACYHRIVETLRRLEDGETLLVQSGKPVGVFRTHPDAPRVLI
                     ANAHLVPRWATADEFRRLDALGLTMYGQMTAGSWIYIGTQGILQGTYETFAECARRDF
                     GGTLAGRLVVTGGLGGMGGAQPLAATMNGAAFLGVDVDASRIERRVATGYCDRMTRSL
                     DEALKWTDEARKARAALSVGLVGNIADVLPDLVRRKVTVDVVTDQTSAHDLRVGYIPA
                     GYDLAAAAEFRVKDPAGYEAKVLDSMQRHVEAWLALKRAGATAFDYGNNLRGQVADRR
                     GMKQAFEVPGFVPEFIRPLFCRGAGPFRWAALSGEPADIYATDAAVLETFPNKEPLAR
                     WIRQAREKVHFQGLPARICWLEYGERATMGERFNWMVKKGVVKAPIVIGRDHLDTGSV
                     ASPNRETEAMKDGSDAIADWPLLNAMLNTACGASWVSLHHGGGVGIGYSIHAGMVVVA
                     DGTDAAGARLQRVLTADPGTGVMRHADAGYEDAIAIANERGVDLPMVNG"
     gene            complement(9621..>10187)
                     /locus_tag="VFU45_10405"
     CDS             complement(9621..>10187)
                     /locus_tag="VFU45_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5359523.1"
                     /translation="GVERGDVVISIDGKPVNRVAELQQDVAFKHPGDEVAVEVARRGG
                     VRKVINVRLTALDSTSGTKLAQNGSGNGDNAPAARNAITPLGITVQTLTSDLADQYQL
                     KDQIQGAVVTDVDPNGPSAQYLADEASGGPDIITAVEGHPVKGDADLRKALQAAGPGT
                     IVTLDVYNARTDSRRLTRIKLAGGSTGS"
BASE COUNT         1353 a         3738 c         3666 g         1430 t
ORIGIN      
        1 gaccggcctg cggggcccgg cggcgggcag cgagcggatc ttcgccgacc gcgtcgaggc
       61 cggcgtcgcg ctggccggtg cgctggcccg gtacgccgac cctcggatgc tggtcctggg
      121 gctggtccgg ggcggcgcgc cggtcgcggc cgaggtggcg cggcgcctcg gcgcggagtt
      181 cgatgtgatc gtcgtgcgga agctcggcgc gcccggcgcg ccggagctcg ccatcggcgc
      241 catcacggcc gacggggcca ccgtcttcaa cgacgacgtg atcggtgagc tggccctcga
      301 ccgtgcacaa gtggccgcgg tgagcgccac gcgccggcgg gaggcggaag agctcgaacg
      361 ggcgttccat cggcggcgcc cgggcgcggc cgtcgagggg cggaccgtca tcctggtcga
      421 cgatggcctc gccaccggcg ccacgatgcg ggccgcggtc gcctcggtgc ggcggcggcg
      481 gcccgggcgc gtggtggtgg cggtgccggt gggttcggtc gaggcgtgcg ccgcgttgcg
      541 cgcggaggcg gacgaggtcg tctgcctgca ggagccggtg catttccagg cagtgggcgc
      601 ctggtatggc gacttccgcc aggtcaccga tggggaggta gcggcgttgc tgcccgaaga
      661 gggttgagcg tcattccggg gcgccggcgt gggccagctc gggggccggc tccggcatct
      721 cggtgagcgt ggcctcggtc cgcagcaggg tccccgccac cgacaccgca ttttccaggg
      781 ccacccgcac caccttcgtc gggtcgacga tcccggcggc caccaggtcg acatacgttc
      841 cgcgggccgc gtcgaggccg atggtgccgt cccccgtccg catccgctcg accgccaccc
      901 cgccgtcgat cccggagttc tcggcgatct ggcgggccgg cgcctcgagg gcgtgccgga
      961 ggatgaggac cccggtgcgc tcgtcgccgt cgaccgcctc ggactcgcgc accacggccg
     1021 gaatgcaccg caggagcgcg agcccgccgc cggggacgat cccttccgcg acggccgcct
     1081 tcgtcgccgc gatcgcgtcc tcgaacgcct cgcgccggct cttgagctcg gtctccgagg
     1141 gcgccccgac gcggatcacg gccacgccgc ccgacagctt ggccagccgt tcctccagct
     1201 tctcgcggtc gtagtcggag gtggtgtcgc ggatctggcc ccgcagctct tcgcaccggg
     1261 ccgcgatggc ggtcgccgca ccggccccgc cgatgatcgt ggtcgagtcg cggtcgacca
     1321 ccacccgctt ggcgccgccg aggtccgcga ccgtcacgtg ctcgagcttg gcgccggtct
     1381 cctcggcgat caccttgccg ccggtcagga tgccgatgtc gtcgagcatc gcgcggcgcc
     1441 ggtccccgaa accgggtgcc ttgaccgcca cgcacgcgag caggccccgg atccggttca
     1501 ccaccagcgt cgccagcgct tcgccctcga cctcctctgc caccaccagc agggaccggt
     1561 tcatcttcgc cacctgctcc aggagcggca ggaggtcctg catgttcgag atccggcggt
     1621 cgtggaggag gatgaacggc tcctggagca ccgcctccat cttggcggcc tcggtcacga
     1681 agtacggcga gaggtagccg cggtcgaact gcatcccctc gaccacttcg acggcggtct
     1741 cggtcccctt ggcctcttcc accgagatga cgccttcggg ccccacccgc tcgaccgcat
     1801 cggccacgag gtcgccggtg atggcgtcgt tgtgcgccga cagtgccgcc acctgcgcct
     1861 tttcgagccg ggtcgtgacc ggccgggaga tctcgcgaag cgccgccacg gccgcccggc
     1921 tcccgcgttc cagcccgcgg cgcaggtcga gggcgctcgc gccggcggcc acgttccgga
     1981 gcccctcggc gaagatcgcg tgcgccagca gcgtcgacgt ggtggtgccg tcgccgacgg
     2041 cctcgccggt ccgggccgcc gcctcccgga gcacctgggc gccgagagtc tcgtcggggt
     2101 cctccaggtc cacttccttg gcgatcgtca cgccgtcgtc gcagacgagc ggctgtcccc
     2161 atttgcgctc gatgagcacg cacttcgagc gcgggccgag cgtcacgcgg acggcatcgg
     2221 ccagggcggc ggcgccggca aggaccttct cccgcgcggc cgaacggaag agaaggcgct
     2281 tgtgcgtcat tctgccgcca cgccggcgcc cgtctgccgg cgggaggagc cgagtgcgac
     2341 ggtcgtcgcc tgccgacccc gctcgccgag ctcctcggca taatgcacct cggatccgac
     2401 cgcgaggcgc tcgaacgccg aatgcaggac gctggcccgg tcgaaataga ccggctcgcc
     2461 gtcctccgcc tggaggaagc cgtacccggc gagcgggtag atctccacca ccgtgcccgt
     2521 gggcgaggca gcctgcggcg gacccacctc ccgcatccgc cgcacgtagt cctgcagctg
     2581 gcgccgcatg gcgtggaatg ccgcgtcgag cgcggtggca agttccgccg ccggctgccg
     2641 atccaccacc agcgcgtggc ccggcacgcg gacctcgagc cgcacgcgcc gatggagggt
     2701 gtcgctccgg cgccggcgct gaggcacgtc gacggtcacg tggcaggcga ggatgcggtc
     2761 gaagtagcgg gccaggtgcg cggcgcgacg gcggatgccc gcctcggccg cgggcgagag
     2821 cccggcgtgg cgcaccgcca gccggaccgg gacggcccgc gccccgggga gatcagtggc
     2881 catggacgag gaacgagacc ttggcgttga cccggaagaa cttgatccgg tcgccctcga
     2941 tctcgccgct gaagttgtcg atccagaccg acttcatgtc cgtgacggtc ttgccgacct
     3001 ccttgaccgc gttctgggcg gcatccgcga aactcttgtc ggattggccg atgacctcga
     3061 cgaccttgac gatgctcgac atttcccgct cctgagattg gacgcaccgg gcgggctgcg
     3121 cggcccccgg cgaacgtcaa cttggcacag gcagcgtgaa gcgggattga gggcggtgcg
     3181 aatcttcggc gaccgttgcg gcgtcgccac ggcgggcggg gcggaggcgg gcgtcagtat
     3241 ccgcgggaga cgtccacggc catggccggc cgctcaccgc gctcgagggc ggtgagcgcg
     3301 tcgaggaacc cgcgggccgc gtgcgccacg gtcgagagtc ccgacacgtg cggggagatg
     3361 acgatgcgcg gatgggccca cagcggcgaa ctggcgggga gcggctccgc ctcgaagacg
     3421 tcgagggccg cgccccggag ggcgcccgct tccaccgcgg cgagcagcgc cggttcatcc
     3481 accaggccgc cgcgcccgac gttgatcagg taggcgccgc ggcaggcggc gaagagcgcg
     3541 gcgccggcga tgtgccaggt ggcctcggtg agtggtgcgg cgaggaccag ccagtcagcg
     3601 ccgccggcca gcgcggggag ctcctccatc cggtgggtgc gggcgaaggg cgcgcgcaga
     3661 gcgccgctcc gcgacacgcc gtcgaccgtg acgccgagtg cagcgatcgc gctggcaatc
     3721 ccgccgccga cgtcgccggt gcccacgatc agcgcccgcg tgccggtgag ggcacgcagg
     3781 tcgcgcgtgt tccaccgccg ggcgcgctgg tcggcctcga aggcgaacag gtcctgggtc
     3841 tcggccagca tccgcgccag gcagtactcg cccatcttgg cgccgaagga ctcgctggtg
     3901 cgggtcagga gcacgtccgc cgggaaatgg cgccggaaga ggaacgcatc gagcccggcg
     3961 ccggtgccgt gcacccacgt cacgtgctcg agctccacct ccgtcggcgg ccggaatccg
     4021 aggtagcagt cggcccatcg caggtcgtcg cgggtgacct cccgccggtc gcggatccgg
     4081 agctcgaggt cggggcgcat cgcccgcagt tcctcggcca gcgcatcgcg gaagtgatcc
     4141 gcgaggagga tccgcctggg tggtccgggg agcgtcacag ggcgtcgtcc gggagcgggg
     4201 tgggcgggcg acaggcatgg acgtgcacca ccgccgtccg tccgtcggtc aggaagcgct
     4261 ggccttcggc attccgcccg ctcgtgacgg cgccggggag gcgcgtgtag ccggagaggc
     4321 ccgcgcatcc gccggcaatg agctcgcgca cgacgaggtc ccgctcggca tcgatatccg
     4381 ggtcgaggcg gtgggtcgcg gtgccgtgga gcggccgcac gaagaggccc gcgtcgcggc
     4441 tggccgccgc cacccacgcc tgctgcgccg tgtcgacgag ccagagccga aggtggtggc
     4501 gctggcgggc gttggatccc tccagctcgt acgccgtgtc ctggcgccgg ccggagaagt
     4561 aatgcgcgct gaccggcgcc ccttcggagc cgcggcggtc agcgatcatc gcagccacct
     4621 cccctgagat ccgcaggagg ttccccgtcg acgcctcggt ccagcccgcc gcacggaagg
     4681 cgctgtccac caggagcgcc gggccgagga cgacgaggtt gatcgggtcg ccggtgaccc
     4741 ggccgcggcg gtcggtcgtc cggggcacga aggtggggag ctggaggggc gtgaggaggt
     4801 gggggaacgc ctcgggcgcg acgcaatccg ccgtggcttc gatcgcaaag gggcgccgca
     4861 gccggaggcc gcccagctcg cccgccacaa tccgcgccgg gtgccgccgc cggatcagcg
     4921 cgtatccgcc gaggatggcg gcggggaaga ggagctcgtc atcgatggcc ccgatcccga
     4981 tcgatgccag cgcccgcccc accttgccgc cccgcttgcc ccccgcggtg acggcgccgc
     5041 tgtcgatcgg ctgcggcgcg tactccagtg aatcgagccc ggcgtcgagc ggcacccagc
     5101 ggccccgcgt gacggcgatg gagtcgaacc ggagcgccag ccggccgcga tgccccgatg
     5161 gcacgaccgc gccgcggacc agggcgaacg gccgcacgac gacgcacgcg tcgtgcagga
     5221 tcgccgccat ggtctggacc gtgaccggag tgcctggacg gtcatgcttc gggcccacgc
     5281 cgcccaggaa ccggatcggg atatgcgtcc ccgccggaac cgacacggtg tcctgcgccg
     5341 ccactgcccg aggcgcgatc ccggacacgg cgaccgccgc gagccacacc accttccgcc
     5401 agctgctcgt catctcatcg cctcagggcc ccgaaaggcc tattcctctg cgcatcggag
     5461 cacgcgacgc gcccgcgtgc cttcccggca tcgatgccga tctccggccg ccctcccacc
     5521 ttcccccgcc ttcccgcgct ttcctccgct tatcctctca ctccctctcc gccagccccc
     5581 ggagcacctg ccatatcgtc acggccgcga gacgtgcggt ccggccatcg acgtcataca
     5641 ccggattgca ctcgaccagg tcgaccgacg tgaccttcgg gctccgcccc gcctggtacg
     5701 cggcgtgaag ccagagatcc acggtcatcc ctcccaccga cggcgcgctc accccgggcg
     5761 ccgccgatcc gtcgaccgcg tcgatgtcga acgaggccat caccggcgcg tcggcctcgg
     5821 cgtagagcgc atcgacgctg gcggcgttca actcggcgtt ccagatccac cgcccccggt
     5881 tgtcgtcgag gaagttgagg tggctcgccg ccaccgactg aggttgcagc cccgccacgg
     5941 tgtagcgggt gcatgcgccc gagggatgga gcagcgcctc acggaacggc gagccggagt
     6001 ggcccttctc gtcgaggagc ttccggacgt ccgggtgcgc gtcccagttg atcacctcga
     6061 ccttgctgcc gacgccggca tagccgagga aatgcccgaa gctcgtctcg tgcccgccgc
     6121 cgaggacgat ggggaagacg ccgtccttga ggtacggcgc cagcacctcg gccagcgcca
     6181 gctggtcggc ggccacgtcg tcggagcact tcaggtcgcc gaggtccagg gtccgttcga
     6241 ccagttcgca atagctctcg tggttctgcg cgtcgggtgt caggcggtag agccaccggc
     6301 ggatctccat cggcgccccc gccgccccga cccggccgcc gttacgatgc acgccctcgt
     6361 ccgacgggaa tccgatgatg accacccgcg gcttggcggc accgttgacg tggccgccca
     6421 ggagatggcc gatgcgcggg tcgccggggg cggtggcggc gttggagatc tcgggacgga
     6481 tgaaggtggc ggtcacggtg cggtgctccg cggtgcgatg gaggtgcggt cccagcgggt
     6541 cacgccgccg atcacggtcc gccggcaggc gttgggacgg aaatggtaga gccattggac
     6601 gacgtccggt gcgtcgatga ccgcgaagtc ggcccgcttg cccggcacga gcgacccgac
     6661 cgacgcgtcc cggccgatgg cgcgcgcggc gaagagcgtg gcacccttga gcgcctcggc
     6721 cggcgccatc cgctgcagcg tgcaggcgag caggagtgcc agcggcaggt ggttgctcgg
     6781 cgccgagccg gggttgaagt cggtcgccac cgccaccggg acgccggcgt cgatcagttt
     6841 gcgggagggc tgcggtgcct ggccgaggta cagcgtcgcg agcggaaggc tcaccgccac
     6901 caccccggac cgcgccatcg cggcgatgcc cgggtcggac acctgctcca ggtggtccgc
     6961 gctggccgcg ccgacctccg ccgcgagctc ggcgccgccg caggaggtca gctggtcggc
     7021 gtggagcttg ggccggagcc cggccgcggt ggcggcccgg aagacggcgc gcgcttcgtc
     7081 gaccgaaaag gcggaccgct cgacgaacac gtcgcagaac tccgccagcc gctcccccgc
     7141 gacccgcggg atcatctcat cgatcaccag cgagacgtat gcggcgcggc ggtcggcgta
     7201 ttccggcggc accgtgtgcg cgcccaggaa ggtcgcgacg atccggcagg ggccgcgctc
     7261 ggccagccgg cggtaggccc ggaggatgcg gagctcggtc tcgaggtcga ggccgtagcc
     7321 gctctttgcc tccacggtgg tcacgccgag cgccaccatc ccggccagga aaccggcggc
     7381 ccgctcgacc aggccgtcct cggggagcgc ccgcgtgcgc cgcatcgtcg acgcgatccc
     7441 gccccccgcc tcggcgatct gcaggtatga ctcgccgcgg atccggcgtt cgaagtcgtc
     7501 ggcccgccag ccgccgaagg ccagatgcgt atggcagtcg atgaggcccg ggatcaccat
     7561 cccgccgccg gcgtcctccc gcggccacct ccggaactcc gctggaagat cacgcgcggc
     7621 gcccgcccac cggatcgtat ccccgtccca cgccagcgcc ccgtcggcga tcgggccgac
     7681 gtcgccctgc cgcgctccgg cgcaggtgta gagggtcgag atgttcgtga ggactggcat
     7741 cgtctcaagg taaggagtga gcggtgagaa gtgagcgggc tgtccttcaa gatctcacca
     7801 ggacgcccgc tcacctctta ccgctgaccg ctcaccgtat catccattca ccatggggag
     7861 gtccaccccc cgctcgttcg cgatcgcgat ggcgtcctcg taccccgcgt cggcgtggcg
     7921 catgacgccc gtgcccgggt ccgccgtgag gacgcgctgg agccgggccc cggcggcgtc
     7981 ggtgccgtcg gccaccacga ccatccccgc gtggatcgaa tacccgatgc cgacgccgcc
     8041 cccgtggtgc agcgacaccc agctcgctcc gcaggcggtg ttgagcatcg cgttgaggag
     8101 cggccagtcg gcgatcgcgt ccgagccgtc cttcatcgcc tcggtctccc ggttgggtga
     8161 agccaccgag ccggtgtcga gatggtcccg cccgatcacg atgggggcct tcaccacacc
     8221 cttcttcacc atccagttga agcgctcccc catcgtggcc cgctcgccgt actcgagcca
     8281 gcagatccgt gccgggaggc cctggaagtg gaccttctcc cgcgcctgcc ggatccagcg
     8341 ggcgagcggc tccttgttgg ggaaggtctc gagcacggcg gcatcggtgg cgtagatgtc
     8401 ggccggctcg ccggagagcg ccgcccagcg gaacgggccc gcgccgcggc agaagagcgg
     8461 acggatgaac tccggcacga atcccggcac ctcgaaggcc tgcttcatcc cgcggcggtc
     8521 cgccacctgg ccccggaggt tgttgccgta atcgaacgcc gtggcacccg cgcgcttgag
     8581 cgcgagccac gcctcgacgt gccgctgcat cgaatccagc accttggcct cgtagcccgc
     8641 cggatccttg acccggaact cggcggcggc cgcaaggtcg tagcccgccg ggatgtaccc
     8701 cacccgcagg tcgtgcgcac tcgtctggtc ggtcaccacg tccaccgtga ccttgcgccg
     8761 gaccaggtcc ggcagcacgt cggcgatgtt ccccaccagc ccgacactga gggccgcgcg
     8821 ggccttgcgc gcctcgtccg tccacttgag tgcttcgtcg agcgaacgcg tcatccggtc
     8881 gcagtagccg gtggccaccc ggcgctcgat ccggctggca tccacgtcga cgccgaggaa
     8941 cgcggcgccg ttcatcgtgg ccgccagggg ctgcgcgccg cccatcccgc cgagcccgcc
     9001 ggtgaccacc aggcggccgg ccagggtgcc gccgaagtcc cgccgggcgc actccgcgaa
     9061 ggtctcgtag gtgccctgca ggatgccctg ggtgccgatg tagatccacg accccgccgt
     9121 catctggccg tacatcgtga gccccagcgc gtcgaggcgg cggaactcgt cggccgtggc
     9181 ccagcgcggg accaggtgcg cgttggcgat cagcacccgc ggcgcgtcgg ggtgggtgcg
     9241 gaagacgccc accggcttgc cgctctggac cagcagcgtc tcgccgtcct ccaggcgccg
     9301 gagcgtctcg acgatccggt ggtagcaggc ccagttccgc gccgccttgc cgccgccccc
     9361 gtagacgatc agctcgtcgg ggtgctcggc caccgccgga tccaggttgt tcatcagcat
     9421 ccggagcgcc gcctcctgca cccacccctt gcagctgagg gtggtgccgg tgggcgcacg
     9481 aacgatctcg ggggcggcgg ctgtggcggt catgcggggc aatgcctgct ggagacgccc
     9541 gaaagatagc ccaccgccgc cccgagacgc gaagggcccg gcgacatcgc cgggcccctc
     9601 ggtcaggtcc tgcaggacgc tcaggagccg gtgctgccgc ccgcgagctt gatgcgggtg
     9661 agccggcggc tgtcggtccg cgcgttgtac acgtcgaggg tcacgatggt gccgggcccc
     9721 gcggcctgca gcgccttccg gaggtcggca tcacccttga ccgggtggcc ctcgaccgcg
     9781 gtgatgatgt cgggcccgcc cgaggcctcg tcggccaggt actgggcgga cggcccgttg
     9841 gggtcgacgt cggtcaccac ggcgccctgg atctggtcct tgagctggta ctggtcggca
     9901 aggtccgagg tgagcgtctg caccgtgatc ccgagcggcg tgatggcgtt gcgggcggcc
     9961 ggggcgttgt cgccgttccc ggatccgttc tgcgccagct tggtgccgct ggtgctgtcc
    10021 aacgctgtca ggcgcacgtt gatgaccttc cggacgccac cgcggcgtgc cacttcgacc
    10081 gccacttcat cgcccgggtg cttgaacgcc acgtcctgct gcagctcggc cacgcggttg
    10141 accggcttgc cgtcgatgct gatgaccacg tcgccccgct cgacgcc
//
